PATENT ABSTRACT
The invention relates to a set of isolated marker genes comprising at least one gene identified as having differential expression as between patients who are responders and non responders to an erbB receptor tyrosine kinase inhibitor; said gene set comprising one or more genes selected from at least the group consisting of the 51 genes listed herein including gene-specific oligonucleotides derived from said genes; and uses of such sets in diagnostic applications.

PATENT DESCRIPTION
RELATED APPLICATIONS  
       [0001]     This application is a continuation of International Application No. PCT/GB2004/002316, which was filed on Jun. 1, 2004, which designated the United States and was published in English, and which claims the benefit of United Kingdom applications GB0312451.8, filed 30 May 2003, GB0322636.2, filed Sep. 26, 2003, and GB0327132.7, filed Nov. 21, 2003. The above mentioned references are incorporated herein by reference.  
       FIELD OF THE INVENTION  
       [0002]     The present invention relates to a method of personalized cancer therapy which employs a set of marker genes to predict whether a patient will respond to a chemotherapeutic agent and a kit for use in said method.  
         [0003]     In particular, the method predicts patient response to erbB tyrosine kinase inhibitors. More particularly the method relates to those patients with cancers mediated alone or in part by erbB tyrosine kinase, especially patients with advanced Non-small Cell Lung Cancer (NSCLC), for example adenocarcinoma, using the levels of a set of marker genes having differential expression between responders and non responders to the erbB tyrosine kinase inhibitor.  
       BACKGROUND TO THE INVENTION  
       [0004]     Lung cancer is the leading cause of cancer death and is therefore a major health problem worldwide. In the treatment of this disease, chemotherapy is the mainstay, because the majority has locally advanced stage 3 (44%) or metastatic stage 4 (32%) disease at diagnosis [1]. Nevertheless, the findings of large meta-analysis revealed that platinum-based chemotherapy contributed to prolong the median survival time of patients with advanced non-small cell lung cancer (NSCLC) by only about 6 weeks compared with best supportive care [2].  
         [0005]     In the last decade, many new cytotoxic agents have been developed including paclitaxel, docetaxel, gemcitabine, and vinorelbine, and have offered multiple choices for patients with advanced lung cancer. However, each regimen served only modest survival benefit compared with the cisplatin-based therapies [3], [4]. More recently, new therapeutic strategies including a number of molecular-targeted agents have been developed in an effort to overcome the limitations of conventional cytotoxic agents [5] [6].  
         [0006]     In recent years it has been discovered that certain growth factor tyrosine kinase enzymes are important in the transmission of biochemical signals which initiate cell replication. They are large proteins which span the cell membrane and possess an extracellular binding domain for growth factors such as epidermal growth factor (EGF) and an intracellular portion which functions as a kinase to phosphorylate tyrosine amino acids in proteins and hence to influence cell proliferation.  
         [0007]     Various classes of receptor tyrosine kinases are known (Wilks,  Advances in Cancer Research,  1993, 60, 43-73) based on families of growth factors which bind to different receptor tyrosine kinases. The classification includes Class I receptor tyrosine kinases comprising the EGF family of receptor tyrosine kinases. This includes receptors for the ligands EGF, TGFα (also referred to as TGFA), amphiregulin (also referred to as AREG), betacellulin, heparin binding EGF, epiregulin and the neuregulins (including NRG-1, NRG-2, NRG-3 and NRG-4). More specifically, these receptors include those with a functional kinase domain called erbB I (EGFR), erbB2 (Neu, Her2) and erbB4 (Her 4), and erbB3 (her3), which does not), Class II receptor tyrosine kinases comprising the insulin family of receptor tyrosine kinases such as the insulin and IGFI receptors and insulin-related receptor (IRR) and Class III receptor tyrosine kinases comprising the platelet-derived growth factor (PDGF) family of receptor tyrosine kinases such as the PDGFα, PDGFβ and colony-stimulating factor 1 (CSF1) receptors.  
         [0008]     It is known that the erbB family of receptor tyrosine kinases, which include EGFR, erbB2, erbB3 and erbB4, are frequently involved in driving the proliferation and survival of tumour cells (reviewed in Olayioye et al.,  EMBO J.,  2000, 19, 3159). One mechanism by which this can occur is over expression of the receptor at the protein level, generally as a result of gene amplification. This has been observed in many common human cancers (reviewed in Klapper et at.,  Adv. Cancer Res.,  2000, 77, 25) such as, non-small cell lung cancers (NSCLCs) including adenocarcinomas (Cerny et al.,  Brit. J. Cancer,  1986, 54, 265; Reubi et al,  Int. J. Cancer  1990, 45, 269; Rusch et al,  Cancer Research,  1993, 53, 2379; Brabender et al,  Clin. Cancer Res.,  2001, 7, 1850) as well as other cancers of the lung (Hendler et al.,  Cancer Cells,  1989, 7, 347.  
         [0009]     As a consequence of the mis-regulation of one or more of these receptors, it is widely believed that many tumours become clinically more aggressive and so correlate with a poorer prognosis for the patient (Brabender et al,  Clin. Cancer Res.,  2001, 7, 1850; Ross et al,  Cancer Investigation,  2001, 19, 554, Yu et al.,  Bioessays,  2000, 22.7, 673). In addition to these clinical findings, a wealth of pre-clinical information suggests that the erbB family of receptor tyrosine kinases are involved in cellular transformation. In addition to this, a number of pre-clinical studies have demonstrated that anti-proliferative effects can be induced by knocking out one or more erbB activities by small molecule inhibitors, dominant negatives or inhibitory antibodies (reviewed in Mendelsohn et al.,  Oncogene,  2000, 19, 6550).  
         [0010]     Thus it has been recognised that inhibitors of these receptor tyrosine kinases should be of value as a selective inhibitor of the proliferation of mammalian cancer cells (Yaish et al.  Science,  1988, 242, 933, Kolibaba et al, Biochimica et Biophysica Acta, 1997, 133, F217-F248; Al-Obeidi et al, 2000,  Oncogene,  19, 5690-5701; Mendelsohn et al, 2000,  Oncogene,  19, 6550-6565). In addition to this pre-clinical data, findings using inhibitory antibodies against EGFR and erbB2 (c-225 and trastuzumab respectively) have proven to be beneficial in the clinic for the treatment of selected solid tumours (reviewed in Mendelsohn et al, 2000,  Oncogene,  19, 6550-6565).  
         [0011]     A number of small molecule inhibitors of erbB family of receptor tyrosine kinases are known, particularly inhibitors of EGF and erbB2 receptor tyrosine kinases. For example European Patent Application No. 0566226 and International Patent Applications WO 96/33980 and WO 97/30034 disclose that certain quinazoline derivatives which possess an anilino substituent at the 4-position possess EGFR tyrosine kinase inhibitory activity and are inhibitors of the proliferation of cancer tissue including prostate cancer. It has been disclosed by J R Woodburn et al. in  Proc. Amer. Assoc. Cancer Research,  1997, 38, 633 and  Pharmacol. Ther.  1999, 82, 241-250 that the compound N-(3-chloro-4-fluorophenyl)-7-methoxy-6-(3-morpholinopropoxy)quinazolin-4-amine is a potent EGFR tyrosine kinase inhibitor. This compound is also known as Iressa (registered trade mark), gefitinib (United States Adopted Name), by way of the code number ZD1839 and Chemical Abstracts Registry Number 184475-35-2. The compound is identified hereinafter as gefitinib. Gefitinib has recently been approved in Japan for the treatment of inoperable or recurrent non-small cell lung cancer (NSCLC) and in the USA as a monotherapy for the treatment of patients with locally advanced metastatic NSCLC after failure of both platinum and docetaxel chemotherapies.  
         [0012]     It is further known from International Patent Application WO 96/30347 that certain structurally-related quinazoline derivatives possessing an anilino substituent at the 4-position also possess EGFR tyrosine kinase inhibitory activity. It has been disclosed in WO 99/55683 that the compound  N -(3-ethynylphenyl)-6,7-bis(2-methoxyethoxy)quinazolin-4-amine, or a pharmaceutically-acceptable salt thereof (linked to the code numbers CP 358774 and OSI-774, identified hereinafter by the code number OSI-774) is an EGFR TKI.  
         [0013]     It is further known from International Patent Application WO 97/38983 that certain other structurally-related quinazoline derivatives possessing an anilino substituent at the 4-position also possess EGFR tyrosine kinase inhibitory activity. It has been disclosed in  J. Med. Chem.,  1999, 42,1803-1815 and WO 00/31048 that the compound 6-acrylamido-N-(3-chloro-4-fluorophenyl)-7-(3 morpholinopropoxy)quinazolin-4-amine (linked to the code numbers PD 183805 and CI 1033, identified hereinafter by the code number CI 1033) is an EGFR TKI.  
         [0014]     It is further known from International Patent Application WO 97/02266 that certain other structurally-related heterocyclic derivatives also possess EGFR tyrosine kinase inhibitory activity. For example, the compound 4-[(1R)-1-phenylethylamino]-6-(4-hydroxyphenyl)-7H-pyrrolo[2,3-d]pyrimidine (linked to the code numbers PKI-166, CGP 75166 and CGP 59326, identified hereinafter by the code number PKI-166) is an EGFR TKI.  
         [0015]     It is further known from European Patent Application No. 0787722 and International Patent Applications WO 98/50038, WO 99/09016 and WO 99/24037 that certain other structurally-related quinazoline derivatives possessing an anilino substituent at the 4-position also possess EGFR tyrosine kinase inhibitory activity. For example, the compound N-[4-(3-bromoanilino)quinazolin-6-yl]but-2-ynamide (linked to the code numbers CL-387785 and EKB-785, identified hereinafter by the code number CL-387785) is an EGFR TKI.  
         [0016]     It is further known from Nature Medicine, 2000, 6, 1024-1028 and U.S. Pat. No. 6,002,008 that certain other structurally-related quinoline derivatives possessing an anilino substituent at the 4-position also possess EGFR tyrosine kinase inhibitory activity. For example, the compound 4-(3-chloro-4-fluoroanilino)-3-cyano-6-(4-dimethylaminobut-2(E)-enamido)-7-ethoxyquinoline (identified hereinafter by the code number EKB-569) is an EGFR TKI.  
         [0017]     It is also known from WO 99/35146 and WO 01/04111 that certain other quinazoline derivatives are inhibitors of one or more of the erbB receptor tyrosine kinase inhibitors. For example the compound N-{3-chloro-4-[(3-fluorobenzyl)oxy]phenyl}-6-[5-({[2-(methylsulfonyl)ethyl]amino}methyl)-2-furyl]quinazolin-4-amine (also identified as lapatinib or GW2016 identified hereinafter by the code GW2016) is thought to be an inhibitor of both EGF and erbB2 receptor tyrosine kinases. Novartis AE788 is another suitable inhibitor compound.  
         [0018]     Inhibition of erbB receptor tyrosine kinase may also be achieved by inhibition of the extracellular ligand binding to a receptor using suitable antibodies against an erbB receptor. For example using the anti-erbB2 antibody trastuzumab [Herceptin™] and the anti-erbb1 antibody cetuximab [C225]). The use of such inhibitory antibodies have proven to be beneficial in the clinic for the treatment of selected solid tumours (reviewed in Mendelsohn et al, 2000,  Oncogene,  19, 6550-6565).  
         [0019]     As mentioned above, gefitinib is an oral active inhibitor of epidermal growth factor receptor-tyrosine kinase (EGFR-TK), which blocks signalling pathways responsible for driving proliferation, invasion, and survival of cancer cells [7]. Potent anti-tumour effects as well as rapid improvements in NSCLC-related symptoms and quality of life have been observed in clinical studies that enrolled patients with advanced NSCLC who did not respond to platinum-based chemotherapy. In the randomized double-blind phase II monotherapy trial (the IDEAL 1 trial), use of gefitinib as 2nd or 3rd line of chemotherapy to advanced NSCLC achieved tumour response rate of 18.4% (95% CI: 11.0-25.9%), and in the IDEAL 2 trial, use as 3rd or 4th line of chemotherapy achieved that of 11.8% (95% CI: 6.2-19.7%) [8],[27],[28].  
         [0020]     Moreover in these trials, the treatment of this drug achieved high disease control rate (54.4% in IDEAL 1, 42.2% in IDEAL 2) and overall symptom improvement rate (40.3% in IDEAL 1, 43.1% in IDEAL 2).  
         [0021]     Those results were promising when compared with responses to conventional cytotoxic agents, but the fact remained that about half of the patients enrolled in these studies received non-effective treatment with no improvement in symptoms. Moreover, the medication exposed non-responders to adverse effects, including life threatening ones such as interstitial pneumonia [11].  
         [0022]     Patients responses to the various chemotherapy treatments differ, therefore there is a need to find methods of predicting which treatment regimes best suit a particular patient.  
         [0023]     There is an increasing body of evidence that suggests that patients responses to numerous drugs may be related to a patients genetic profile and that determination of the genetic factors that influence, for example, response to a particular drug could be used to provide a patient with a personalised treatment regime. Such personalised treatment regimes offer the potential to maximise therapeutic benefit to the patient, whilst minimising, for example side effects that may be associated with alternative and less effective treatment regimes. There is therefore a need for methods that can predict a patients response to a drug.  
       SUMMARY OF THE INVENTION  
       [0024]     It has been found that the sensitivity of certain cancers to chemotherapeutic agents can be predicted by gene expression and hence that the suitability of cancer patients for treatment with such chemotherapeutic agents can be determined by measuring the relative levels of particular genes in patient tissue.  
         [0025]     Accordingly, the present invention provides an isolated set of marker genes comprising at least one gene identified as having differential expression as between patients who are responders and non responders to an erbB receptor tyrosine kinase inhibitor, said gene set comprising one or more genes selected from at least the group consisting of the 51 genes listed in Table 4 herein including gene-specific oligonucleotides derived from said genes. In Table 4, accession numbers are given for the genes on the GenBank database.  
         [0026]     Sequences of these genes are described in Table 4a and Table 4b. As will be appreciated by those skilled in the art, sequences available at the given accession numbers represent only examples of sequences of the genes referred to in the table; alternative sequences, including sequences which comprise sequencing error corrections, allelic or other variations, splice mutants and the like are also included in the definition of the gene represented by the name used. In a most preferred embodiment, the sequences referred to are the sequences set forth at the accession numbers and specific sequences given and set out in detail in Table 4a.  
         [0027]     In a further aspect the present invention provides a set of isolated marker genes comprising at least one gene identified as having differential expression as between patients who are responders and non responders to an erbB receptor tyrosine kinase inhibitor; said gene set selected from the group consisting of the 51 genes listed in Table 4 herein including gene-specific oligonucleotides derived from said genes.  
         [0028]     The present invention permits the improved prognosis and hence quality of life of cancer patients by matching the treatments to individual patients and so making more effective use of the types of drug available.  
         [0029]     A preferred set is at least one or more of the first 40 genes listed in Table 4 herein.  
         [0030]     A further preferred set is at least one or more of the first 20 genes listed in Table 4 herein.  
         [0031]     A further preferred set is at least one or more of the first 12 genes listed in Table 4 herein.  
         [0032]     A preferred set is at least one or more of the first 5 genes listed in Table 4 herein.  
         [0033]     An especially preferred set is the first 12 genes listed in Table 4a herein, namely FLJ22622 (e.g. GenBank NM — 024829), AREG (e.g. GenBank BC009799), C0R01C (e.g. GenBank NM — 014325), AVEN (e.g. GenBank BC010488), DUSP3 (e.g. GenBank NM — 004090, DJ473B4 (e.g. GenBank AI026836), PHLDA2 (e.g. GenBank BU500509), RBM7 (e.g. GenBank NM — 0106090), EST (GenBank BX0952512), OSMR (e.g. GenBank AI436027), GCLC (e.g. GenBank AI971137), COL4A3BP (e.g. GenBank BQ024877).  
         [0034]     Preferably the inhibitor is selected from gefitinib, OSI-774, PKI-166, EKB-569, GW2016, CI-1033 and an anti-erbB antibody such as trastuzumab and cetuximab.  
         [0035]     Most preferably the inhibitor is gefitinib.  
         [0036]     The present invention is particularly suitable for use in predicting the response to the aforementioned chemotherapeutic agents in those patients or patient population with a cancer mediated alone, or in part, by an erbB tyrosine kinase. Such cancers include, for example, non-solid tumours such as leukaemia, multiple myeloma or lymphoma, and also solid tumours, for example bile duct, bone, bladder, brain/CNS, breast, colorectal, cervical, endometrial, gastric, head and neck, hepatic, lung, muscle, neuronal, oesophageal, ovarian, pancreatic, pleural/peritoneal membranes, prostate, renal, skin, testicular, thyroid, uterine and vulval tumours.  
         [0037]     The present invention is particularly suitable for identifying those patients with NSCLC, more particularly advanced NSCLC including advanced adenocarcinoma that will respond to treatment with chemotherapeutic agents such as an erbB receptor tyrosine kinase inhibitor as hereinbefore defined.  
         [0038]     The present invention offers considerable advantages in the treatment of cancers such as NSCLC, especially advanced NSCLC by identifying “individual cancer profiles” of NSCLC and so determining which tumours would respond to gefitinib. This includes 1 st  line treatment and any other treatment regimen, such as, for example chemotherapy failed patients.  
         [0039]     The present invention is particularly useful in the treatment of patients with advanced NSCLC who have failed previous chemotherapy, such as platinum-based chemotherapy.  
         [0040]     The present invention is also particularly useful in the treatment of patients with locally advanced (stage lIIB) or metastasized (stage IV) NSCLC who have received previous chemotherapy, such as platinum-based chemotherapy.  
         [0041]     The present invention also provides a method of predicting the responsiveness of a patient or patient population with cancer-, for example lung cancer, to treatment with chemotherapeutic agents, especially erbB receptor tyrosine kinase inhibitors, comprising comparing the differential expression of a set of marker genes said marker genes selected from the gene sets as defined above.  
         [0042]     Preferably the assessment of expression is performed by gene expression profiling using oligonucleotide-based arrays or cDNA-based arrays of any type; RT-PCR (reverse transcription—Polymerase Chain Reaction), real-time PCR, in-situ hybridisation, Northern blotting, Serial analysis of gene expression (SAGE) for example as described by Velculescu et al Science 270 (5235): 484-487, or differential display. Details of these and other methods can be found for example in Sambrook et al, 1989, Molecular Cloning: A Laboratory Manual). Preferably the assessment uses a microarray assay.  
         [0043]     Alternatively, or in addition, the assessment uses an immunohistochemical assay.  
         [0044]     In a further aspect, the present invention provides a kit for use in a method of predicting the responsiveness of a patient or patient population with cancer, to treatment with chemotherapeutic agents, especially erbB receptor tyrosine kinase inhibitors, comprising a marker gene set as defined above on a suitable support medium. Preferably the marker gene is attached to a support material or membrane such as nitrocellulose, or nylon or a plastic film or slide.  
         [0045]     Preferably the kit comprises a microarray. 
     
    
     BRIEF DESCRIPTION OF THE FIGURES  
       [0046]      FIG. 1 : Images illustrating laser-microbeam microdissection of four representative lung adenocarcinomas. The upper row shows the samples before dissection; the lower row, dissected cancer cells (H.E. stain X100). TBB indicates transbronchial biopsy; LN, lymph-node.  
         [0047]      FIG. 2 : Establishing a scoring system to predict the efficacy of gefitinib treatment.  
         [0048]     A. Different prediction scores appear when the number of discriminating genes is changed. The number of the discriminating gene sets (from 5 to 51) corresponds to the number of selected genes from the top of the rank-ordered list in table 4. A larger value of classification score (CS) indicates better separation of the two groups.  
         [0049]     B. Hierarchical clustering of 17 “learning” cases using 51 candidate genes for gefitinib-sensitivity (left), and 12 prediction genes that were finally selected for the GRS (right). The dendrograms represent similarities in expression patterns among individual cases; longer branches indicate greater differences. The two groups were most clearly separated by the 12-gene set.  
         [0050]     C. Schematic distinction of responder, non-responder and “test cases” verified on the basis of the GRS. Red diamonds denote prediction scores for learning PR cases and blue diamonds represent learning PD cases. A pink triangle indicates a test PR case that had not been used for establishing GRS, and blue triangles indicate test PD cases. Yellow triangles indicate test SD cases that kept the SD status throughout the 4-month observation period, and green triangles indicate test cases once judged as SD at a certain-time point of the study but showed progression of the disease within three or four months after the start of treatment.  
         [0051]      FIG. 3 : Validation of GRS with semi-quantitative RT-PCR and immunohistochemical analyses.  
         [0052]     A. Representative image of semi-quantitative RT-PCR analysis of RNAs from the PR and PD groups. OSMR and GCLC genes were over-expressed in non-responders (PD). The integrity of each cDNA template was controlled through amplification of ACTB.  
         [0053]     B. Immunohistochemical staining of representative samples from fiberscopic transbronchial biopsy (TBB) and lymph-node (LN) biopsy from the same PD-patient (No. LC21), using anti-AREG antibody (X 200).  
         [0054]     C. Immunohistochemical staining of representative samples from PD patients, using antibodies for other 4 prediction markers (TGFA, ADAM9, CD9, and OSMR) (X200).  
         [0055]      FIG. 4 : Serologic concentration of TGFA determined by ELISA in 5 PR, 10 SD, and 20 PD adenocarcinoma cases. The averaged serum levels of TGFA were shown as black bars: 19·0±2·8 pg/ml (mean±SE) in PD patients, 13·9±1·9 pg/ml in SD patients, and 12·8±1·4 pg/ml in PR patients.  
         [0056]      FIG. 5 : Anti-apoptotic effect of secreted AREG on gefitinib-sensitive PC-9 cells.  
         [0057]     A. Expression of AREG transcript examined by semi-quantitative RT-PCR in lung-adenocarcinoma cell lines PC-9, NCI-H358, and -H522.  
         [0058]     B. PC-9 cells cultured in medium supplemented with 10% FCS, in serum-free medium, or in serum-free conditioned medium (CM) obtained from cultures of NCI-H358 or -H522 cells. Each medium was replaced once with the same medium at the 48-hour time point; 72 hours after adding gefitinib at concentrations of 0·5 or 1·0 μM, cell viability was measured by MTT assays. The experiments were done in triplicate. The Y-axis indicates the relative MTT value (MTT in the presence of 0·or 1·0 μM gefitinib/MTT in the absence of gefitinib) of the cells incubated in different media.  
         [0059]     C. Effect of AREG, secreted in an autocrine manner, on the resistance of NSCLC cells to gefitinib. At the start of culture, PC-9 cells were inoculated into medium containing 1·0 μM gefitinib and recombinant AREG protein (final concentrations of 1-100 ng/ml); 72 hours later, cell viability was measured by triplicate MTT assays (blue bars). The Y-axis indicates the relative MTT values (MTT at individual concentrations of AREG/MTT without AREG) of the cells.  
         [0060]     Effect of AREG on the viability of NSCLC cells in the absence of 1·0 μM gefitinib was also studied. Individual PC-9 cells were added to medium containing recombinant AREG protein but no gefitinib; 72 hours later, viability was measured by triplicate MTT assays (red bars).  
         [0061]      FIG. 6 : Immunohistochemical analysis of amphiregulin expression in sections derived from PD and PR patients. 
     
    
     DETAILED DESCRIPTION  
       [0062]     The invention will be described in more detail and illustrated by the following examples which are meant to serve to assist one of ordinary skill in the art in carrying out the invention and are not intended in any way to limit the scope of the invention. Certain elements of the invention are also described in more detail below.  
         [0063]     “Set of Isolated Marker Genes” 
         [0064]     These are, according to the context of the embodiments described herein, a group of genes which can be used in classification or categorisation of patent response according to the invention.  
         [0065]     “Differential Expression” 
         [0066]     Genes that are either expressed at a higher or lower level as between groups of responders or nonresponders.  
         [0067]     “Responders/Non Responders” 
         [0068]     Objective tumour responses according to Union International Contre le Cancer/World Health Organization (U ICC/WHO) Criteria are categorised as follows: complete response (CR): no residual tumour in all evaluable lesions; partial response (PR): residual tumour with evidence of chemotherapy-induced 50% or greater decrease under baseline in the sum of all measurable lesions and no new lesions; stable disease (SD) residual tumour not qualified for CR; and progressive disease (PD): residual tumour with evidence of 25% or greater increase under baseline in the sum of all measurable lesions or appearance of new lesions. As defined herein, non responders are PD.  
         [0069]     The present invention is particularly effective for determining those patients which are CR or PR  
         [0070]     “ErbB Receptor Inhibitors Including, Without Limitation, ErbB Receptor Tyrosine Kinase Inhibitors” 
         [0071]     This family includes EGF, erbB2 (HER), erbB3 (note that erbB3 does not have a functional kinase domain) and erbB4 as described in the background to the invention above.  
         [0072]     “Gene-Specific Oligonucleotides” 
         [0073]     These are intended to be unique to the respective genes so that, for example, fragments of the gene that uniquely identify the gene. Advantageously, a gene-specific oligonucleotide is between 5 and 50 nucleotides in length, preferably about 15 to 30 nucleotides, and most preferably about 23 nucleotides.  
         [0074]     “Arrays or Microarrays” 
         [0075]     Array technology and the various techniques and applications associated with it are described generally in numerous textbooks and documents. Gene array technology is particularly suited to the practice of the present invention. Methods for preparing microarrays are well known in the art. These include Lemieux et al., (1998),  Molecular Breeding  4, 277-289, Schena and Davis.  Parallel Analysis with Biological Chips.  in  PCR Methods Manual  (eds. M. Innis, D. Gelfand, J. Sninsky), Schena and Davis, (1999),  Genes, Genomes and Chips. In DNA Microarrays: A Practical Approach  (ed. M. Schena), Oxford University Press, Oxford, UK, 1999),  The Chipping Forecast  ( Nature Genetics  special issue; January 1999 Supplement), Mark Schena (Ed.),  Microarray Biochip Technology,  (Eaton Publishing Company), Cortes, 2000,  The Scientist  14[17]:25, Gwynne and Page,  Microarray analysis: the next revolution in molecular biology, Science,  Aug. 6, 1999; and Eakins and Chu, 1999,  Trends in Biotechnology,  17, 217-218.  
         [0076]     The technology is described in PCT/US01/10063 and US 2002 090979 and references therein.  
         [0077]     Commercial suppliers include Affymetrix (California) and Clontech Laboratories (California).  
         [0078]     Major applications for array technology include the identification of sequence (nucleotide sequence/nucleotide sequence mutation) and the determination of expression level (abundance) of nucleotide sequences. Gene expression profiling may make use of array technology, optionally in combination with proteomics techniques (Celis et al, 2000,  FEBS Lett,  480(1):2-16; Lockhart and Winzeler, 2000, Nature 405(6788):827-836; Khan et al., 1999, 20(2):223-9). Other applications of array technology are also known in the art; for example, nucleotide sequence discovery, cancer research (Marx, 2000, Science 289: 1670-1672; Scherf, et al, 2000, Nat Genet;24(3):236-44; Ross et al, 2000, Nat Genet. March 2000; 24(3):227-35), SNP analysis (Wang et al, 1998, Science, 280(5366):1077-82), drug discovery, pharmacogenomics, disease diagnosis (for example, utilising microfluidics devices: Chemical &amp; Engineering News, Feb. 22, 1999, 77(8):27-36), toxicology (Rockett and Dix (2000),  Xenobiotica,  30(2):155-77; Afshari et al., 1999, Cancer Res1;59(19):4759-60) and toxicogenomics (a hybrid of functional genomics and molecular toxicology). The goal of toxicogenomics is to find correlations between toxic responses to toxicants and changes in the nucleotide sequencetic profiles of the objects exposed to such toxicants (Nuwaysir, et al (1999),  Molecular Carcinonucleotide sequencesis,  24:153-159).  
         [0079]     In general, any library may be arranged in an orderly manner into an array, by spatially separating the members of the library. Examples of suitable libraries for arraying include nucleic acid libraries (including DNA, nucleotide sequence, oligonucleotide, etc libraries), peptide, polypeptide and protein libraries, as well as libraries comprising any molecules, such as ligand libraries, among others. Accordingly, where reference is made to a “library” such reference includes reference to a library in the form of an array.  
         [0080]     The members of a library are generally fixed or immobilised onto a solid phase, preferably a solid substrate, to limit diffusion and admixing of the samples. In particular, the libraries may be immobilised to a substantially planar solid phase, including membranes and non-porous substrates such as plastic and glass. Furthermore, the samples are preferably arranged in such a way that indexing (i.e. reference or access to a particular sample) is facilitated. Typically the samples are applied as spots in a grid formation. Common assay systems may be adapted for this purpose. For example, an array may be immobilised on the surface of a microplate, either with multiple samples in a well, or with a single sample in each well. Furthermore, the solid substrate may be a membrane, such as a nitrocellulose or nylon membrane (for example, membranes used in blotting experiments). Alternative substrates include glass, or silica based substrates. Thus, the samples are immobilised by any suitable method known in the art, for example, by charge interactions, or by chemical coupling to the walls or bottom of the wells, or the surface of the membrane. Other means of arranging and fixing may be used, for example, pipetting, drop-touch, piezoelectric means, ink-jet and bubblejet technology, electrostatic application, etc. In the case of silicon-based chips, photolithography may be utilised to arrange and fix the samples on the chip. The samples may be arranged by being “spotted” onto the solid substrate; this may be done by hand or by making use of robotics to deposit the sample. In general, arrays may be described as macroarrays or microarrays, the difference being the size of the sample spots. Macroarrays typically contain sample spot sizes of about 300 microns or larger and may be easily imaged by existing gel and blot scanners. The sample spot sizes in microarrays are typically less than 200 microns in diameter and these arrays usually contain thousands of spots. Thus, microarrays may require specialised robotics and imaging equipment, which may need to be custom made. Instrumentation is described generally in a review by Cortese, 2000,  The Scientist  14[11]:26.  
         [0081]     Techniques for producing immobilised libraries of DNA molecules have been described in the art. Generally, most prior art methods describe how to prepare single-stranded nucleic acid molecule libraries, using for example masking techniques to build up various permutations of sequences at the various discrete positions on the solid substrate. U.S. Pat. No. 5,837,832 describes an improved method for producing DNA arrays immobilised to silicon substrates based on very large scale integration technology. In particular, U.S. Pat. No. 5,837,832 describes a strategy called “tiling” to prepare specific sets of probes at spatially-defined locations on a substrate which may be used to produced the immobilised DNA libraries of the present invention. U.S. Pat. No. 5,837,832 also provides references for earlier techniques that may also be used.  
         [0082]     To aid detection, targets and probes may be labelled with any readily detectable reporter such as a fluorescent, bioluminescent, phosphorescent, radioactive reporter. Labelling of probes and targets is disclosed in Shalon et al., 1996,  Genome Res  6(7):639-45.  
         [0083]     The materials for use in the methods of the present invention are ideally suited for preparation of kits. A set of instructions will typically be included.  
         [0000]     General Recombinant DNA Methodology Techniques  
         [0084]     The present invention employs, unless otherwise indicated, conventional techniques of chemistry, molecular biology, microbiology, recombinant DNA and immunology, which are within the capabilities of a person of ordinary skill in the art. Such techniques are explained in the literature. See, for example, J. Sambrook, E. F. Fritsch, and T. Maniatis, 1989,  Molecular Cloning: A Laboratory Manual,  Second Edition, Books 1-3, Cold Spring Harbor Laboratory Press; Ausubel, F. M. et al. (1995 and periodic supplements;  Current Protocols in Molecular Biology,  ch. 9, 13, and 16, John Wiley &amp; Sons, New York, N.Y.); B. Roe, J. Crabtree, and A. Kahn, 1996,  DNA Isolation and Sequencing: Essential Techniques,  John Wiley &amp; Sons; M. J. Gait (Editor), 1984,  Oligonucleotide Synthesis: A Practical Approach,  Irl Press; and, D. M. J. Lilley and J. E. Dahlberg, 1992,  Methods of Enzymology: DNA Structure Part A: Synthesis and Physical Analysis of DNA  Methods in Enzymology, Academic Press. Each of these general texts is herein incorporated by reference.  
         [0085]     In a specific embodiment of the invention, a cDNA microarray system representing 27, 648 genes was used to select a set of genes predicating the responsiveness to gefitinib for advanced NSCLC. Statistical analysis of the expression profiles identified dozens of genes differentially expressed between responders and non-responders to gefitinib. A drug response scoring (DRS) system based on the expression of these genes successfully predicted the response to gefitinib therapy.  
         [0000]     Materials and Methods  
         [0000]     Patients and Tissue Samples  
         [0086]     A phase II clinical study was carried out comprising a multi-center trial to explore the dominant biological factors responsible for clinical anti-tumor effect, adverse drug reactions (ADR) and pharmacokinetics of ZD1839 dosed 250 mg daily in patients with advanced non-small-cell lung cancer who have failed previous chemotherapy. The primary endpoint was to clarify a gene-expression profile that could determine in advance a potential anti-tumor effect of gefitinib. At the start of the study, the sample size was estimated using studies conducted thus far as a rationale. 12,13  Since the response rate for gefitinib has been less than 20% in patients with lung cancer, 8-10  about 50 patients were estimated to be required to obtain learning cases estimated above. Patients whose locally advanced (stage IIIB) or metastasized (stage IV) NSCLCs were resistant to one or more regimens of conventional chemotherapy were enrolled in this trial. Inclusion criteria were (1) age greater than 20 years, (2) Performance Status (PS) 0-2, (3) adequate liver and kidney function tests. All patients were treated with 250 mg of gefitinib orally once a day at the Tokushima University or Kinki University hospitals in Japan. The treatment was continued until the patient was dropped from the study due to (1) progression of disease, (2) intolerable toxicity, or (3) withdrawal of consent.  
         [0087]     Objective tumor responses were assessed every 4 weeks after the beginning of treatment, according to criteria outlined by the  Union International Contre le Cancer/World Health Organization  (UICC/WHO). Response categories were as follows: complete response (CR), no residual tumor in any evaluable lesion; partial response (PR), residual tumor with evidence of 50% or greater decrease under baseline in the sum of all measurable lesions, and no new lesions; progressive disease (PD), residual tumor with evidence of 25% or greater increase under baseline in the sum of all measurable lesions, or appearance of new lesions; and stable disease (SD), residual tumor not qualified for CR, PR, or PD. All evaluable lesions were measured bi-dimensionally (sum of products of longest diameter and its longest perpendicular of measurable lesions) using the same techniques as baseline, e.g. plain X-ray, CT, or MRI.  
         [0088]     At the end of 4-month treatment (or withdrawal), the best overall response was evaluated for each patient based on definitions as follows: CR, patients who qualified for CR at two sequential examination points with an interval of at least 28 days between them; PR, patients judged as PR or better at two sequential examination points with an interval of at least 28 days between them; SD, patients who were SD or better at two sequential examination points at least 28 days apart but who did not qualify as CR or PR. The first judgment of an SD case must be done at or after the first tumor assessment point (28 days after randomization); PD, the patients determined as PD at or before the first tumor assessment point (28 days after randomization); Unknown, the patient does not qualify for a best response of increased disease, and all objective statuses after baseline (before randomization) and before progression are unknown.  
         [0089]     Prior to the gefitinib treatment, tumor specimens were taken by trans-bronchial (TBB), skin, or lymph-node biopsy with written informed consent from each patient. Ethics approval was obtained from the ethics committee of the individual institutes. Biopsy samples were frozen immediately, embedded in TissueTek OCT medium (Sakura, Tokyo, Japan), and stored at −80° C. All samples were examined microscopically, and samples from 28 patients (17 learning and 11 test cases) that contained enough cancer cells for analysis of expression profiles were initially selected for further analysis. For validation of the prediction system, a blinded set of samples from 5 newly enrolled cases (4 PD and 1 SD) were also added to the 11 test cases. Clinical and histological information about these patients is summarized in Table 1-3.  
         [0000]     Microdissection  
         [0090]     In view of significant differences in the proportions of cancer cells and various types of parenchymal cells that are present from one tumor to another, microdissection is a necessary means of obtaining precise gene-expression profiles on cDNA microarrays. Therefore we stained 8 μm-thick frozen sections with hematoxylin and eosin and collected cancer cells selectively, using the μCUT laser-microbeam microdissection system (Molecular Machines &amp; Industries AG, Glattbrugg, Switzerland). 14  In this system tissue sections are mounted on a thin supporting polyethylene membrane that will be cut together with the target tissue; a pulsed-ultraviolet (UV) narrow-beam-focus laser cuts out cancer cells along a pre-selected track that can be observed on a video screen. The material to be extracted is never directly exposed to the laser but only circumscribed by it; unlike other LMM systems, this one allows recovery of dissected cells to proceed without radiation. Moreover, the membrane protects the tissue on the slide against cross-contamination. Using this system we were able to isolate small areas of tissue rapidly, and to isolate single cells from histological sections ( FIG. 1 ).  
         [0000]     RNA Extraction and T7-Based RNA Amplification  
         [0091]     Total RNA was extracted from individual microdissected populations of cancer cells using RNeasy mini kits and RNase-free DNase kits (QIAGEN, Hilden, Germany) according to the manufacturer&#39;s protocols. Total RNAs were subjected to T7-based RNA amplification, as described previously. 15  Two rounds of amplification yielded 40-200 μg of aRNA (amplified RNA) (&gt;100,000-fold) from each sample. As a control probe, normal human lung poly(A) + RNA (BD Biosciences Clontech, Palo Alto, Calif. and BIOCHAIN, Hayward, Calif., USA) was amplified in the same way. Aliquots (2·5 μg) of mRNA from individual samples and from the control were reversely transcribed in the presence of Cy5-dCTP and Cy3-dCTP respectively.  
         [0000]     cDNA Microarray  
         [0092]     Our “genome-wide” cDNA microarray system contains 27,648 cDNAs selected from the UniGene database of the National Center for Biotechnology Information. 15  Fabrication of the microarray, hybridization, washing, and detection of signal intensities were described previously. 15  To normalize the amount of mRNA between tumors and controls, the Cy5/Cy3 ratio for each gene&#39;s expression was adjusted so that the averaged Cy5/Cy3 ratio of 52 housekeeping genes was equal to one. We assigned a cutoff value to each microarray slide using analysis of variance, and the Cy5/Cy3 ratio of the gene was calculated as follows: (1) if Cy5 (cancer sample) was lower than the cut off level, then the Cy5/Cy3 ratio of the gene was substituted by 2-5 percentile among the Cy5/Cy3 ratios of other genes whose Cy5 and Cy3 were higher than the cut off level; (2) if Cy3 (control sample) was lower than the cut off level, then the Cy5/Cy3 ratio of the gene was substituted by 97·5 percentile among the Cy5/Cy3 ratios of other genes whose Cy5 and Cy3 were higher than the cut off level; (3) if both Cy5 and Cy3 were lower than the cut off level, then the Cy5/Cy3 ratio of the gene was left blank.  
         [0000]     Extraction of Genes for Predicting Responsiveness to Gefitinib  
         [0093]     To discover genes that might be associated with sensitivity to gefitinib, individual measurements of about 27,648 genes were compared between the two groups of patients, one classified as responders to gefitinib (PR) and the other as non-responders (PD). To reduce the dimensionality of the number of potent genes that could discriminate between the two classes, we extracted only genes that fulfilled two criteria: 1) signal intensities were higher than the cut-off level in at least 60% of either group, and 2) 1 MED PR −MED PD |≦1, where MED indicates the median calculated from log-transformed relative expression ratios in each group. Then random-permutation tests were applied to estimate the ability of individual genes to distinguish between the two classes (PR and PD); mean (μ) and standard deviations (σ) were calculated from the log-transformed relative expression ratios of each gene in both groups. A discrimination score (DS) for each gene was defined as follows: 
 
 DS =(μ PR −μ PD )/(           PR +σ PD ). 
 
         [0094]     The samples were randomly permutated 10,000 times for each pair of groups. Since the DS dataset of each gene showed a normal distribution, we calculated a p-value for the user-defined grouping.  
         [0095]     Calculation of Drug-Response Scores  
         [0096]     We calculated the drug response scores for gefitinib (gefitinib response scores, or GRS) reflecting the expression levels of candidate prediction-genes according to procedures described previously. 16-18  Each gene (gi) votes for either responder (PR) or non-responder (PD) depending on whether the expression level (xi) in the sample is closer to the mean expression level of one group or the other in reference samples. The magnitude of the vote (vi) reflects the deviation of the expression level in the sample from the average of the two classes: 
 
 Vi=|xi −(μ PR +μ PD )/2|. 
 
         [0097]     We summed the votes to obtain total votes for responders (V PR ) and non-responders (V PD ), and calculated GRS values as follows: GRS=((V PR −V PD )/(V PR +V PD ))×100, where the GRS value reflects the margin of victory in the direction of either responder or non-responder. GRS values range from −100 to 100; the higher an absolute value of GRS, the stronger the prediction.  
         [0000]     Cross-Validation of Scores and Evaluation of the Prediction System  
         [0098]     The prediction scores of all samples were obtained by a leave-one-out approach, in which one sample at a time was removed from the sample set; permutational p-values and mean values of the two classes were calculated for each gene using the remaining samples. The drug-response of the withheld sample was predicted by calculating the prediction score.  
         [0099]     These procedures were repeated for each sample. 16-17    
         [0100]     To evaluate the reliability of the prediction system, we calculated a “classification score” (CS) using the GRS values of responders and non-responders in each gene set, as follows: 
 
 CS =(μ GRSpr −μ GRSpd )/(           GRSpr +           GRSpd ). 17  
 
         [0101]     A larger value of CS indicates better separation of the two groups by the prediction system.  
         [0000]     Hierarchical Clustering  
         [0102]     We used web-available software (“Cluster” and “TreeView”) written by M. Eisen (http://genome-www5.stanford.edu/MicroArray/SMD/restech.html) to create a graphic representation of the microarray data and to create a dendrogram of hierarchical clustering. Before the clustering algorithm was applied, the fluorescence ratio for each spot was first log-transformed and then the data for each sample were median-centered to remove experimental biases.  
         [0000]     Semi-Quantitative RT-PCR Analysis  
         [0103]     Aliquots (5·0 μg) of the same aRNA hybridized to the microarray slides from individual samples and from the normal control lung were reversely transcribed using oligo(dT) 12-18  primer and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, Calif., USA). Semi-quantitative RT-PCR experiments were carried out with the following sets of synthesized primers specific to the 12 top-ranked genes used for establishing a GRS or with beta-actin (ACTB)-specific primers as an internal control: FLJ22662, 5′-GCCATAAGTGGTCCCACAGT-3′ and 5′-GTCTTCTAGTCCGTCATCTCCCT-3′; Amphiregulin (AREG), 5′-CCATAGCTGCCTTTATGTCTGC-3′ and 5′-CTTTTTACCTTCGTGCACCTTT-3′, coronin, actin binding protein, IC (COROIC), 5′-TAATCTGCTGAGGACCTTTTGTC-3′ and 5′-TAATTCACTGTCCTCTTCTGGGA-3′; apoptosis, caspase activation inhibitor (AVEN), 5′-GCTCACAGCAGTAAATGCCTA-3′ and 5′-TGCTATGCTGTAAACACTGGCTA-3′; dual specificity phosphatase 3 (DUSP3), 5′-GGATCCTTTATTGGTGGTAGAGC-3′ and 5′-CCAGAGTGACCCTGAAGATAAAT-3′; DJ473B4, 5′-ACCTGATTCTCTAGGTGCAGTTT-3′ and 5′-GTCGTTTCAACCAGGTAGTTTTG-3′; pleckstrin homology-like domain, family A, member 2 (PHLDA2), 5′-GGGCGCCTTAAGTTATTGGA-3′ and 5′-GGATGGTAGAAAAGCAAACTGG-3′; RNA binding motifprotein 7 (RBM7), 5′-TGTAATGGAGATTGTACAGGTTG-3′ and 5′-AGGAACAGTACAAATGCTGTGGT-3′; BX092512 (EST), 5′-GCACTCCTTGAAGGTACACTAAC-3′ and 5′-ATTTGTATTCACTCAGCCATGC-3′; oncostatin M receptor (OSMR), 5′-ACCCAACTTCAAAACTAGGACTC-3′ and 5′-ACAGCTTGATGTCCTTTCTATGC-3′, glutamate-cysteine ligase, catalytic subunit (GCLC), 5′-TCATGAAAGGCACTGAGTTTTG-3′ and 5′-GTTAGCTGAAGCAGCTTTATTGC-3′; collagen, type IV, alpha 3 binding protein (COL4A3BP), 5′-ATATGCACAATCCTGGAAGTGA-3′ and 5′-TGCCTTACTAGCATTACCACCAT-3′; ACTB, 5′-GAGGTGATAGCATTGCTTTCG-3′ and 5′-CAAGTCAGTGTACAGGTAAGC-3′. PCR reactions were optimized for the number of cycles to ensure product intensity within the logarithmic phase of amplification. We did phosphor imager quantification analysis (Molecular Imager FX: Bio-Rad Laboratories, Hercules, Calif., USA), and RT-PCR band intensities were quantitatively compared with normalized Cy5/Cy3 ratio of gene expression from the microarray data.  
         [0104]     RT-PCR was performed to screen the mutation at entire region of codon 709-870 (from p-loop to activation loop) of EGFR which was recently reported as a hot spot of mutation, 18  using three primer sets: fragment-1,5′-TCTTACACCCAGTGGAGAAGC-3′ and 5′-GTCTTTGTGTTCCCGGACAT-3′; fragment-2,5′-ACTATGTCCGGGAACACAAA-3′ and 5′-TTCCGTCATATGGCTTGG-3′; fragment-3,5′-CGTCGCTATCAAGGAATTAAGAG-3′ and 5′-GTAGCTCCAGACATCACTCTGGT-3′. RT-PCR products from 19 NSCLC patients treated with gefitinib were analyzed by direct sequencing.  
         [0000]     Immunohistochemical Analysis  
         [0105]     To confirm the differential expression of AREG and transforming growth factor-alpha (TGFA) proteins, both of which encode the ligand for EGFR and other ERBB members, and other 3 candidate markers (a disintegrin and metalloproteinase domain 9 (ADAM9), D9 antigen (p24), and OSMR), which are also known to relate to the EGFR signalling, for predicting responders vs non-responders to gefitinib, we stained clinical tissue sections obtained by fiberscopic transbronchial biopsy (TBB) and lymph-node biopsy using ENVISION+ Kit/HRP (DakoCytomation, Glostrup Denmark). Briefly, after endogenous peroxidase and protein blocking reactions, anti-human AREG polyclonal antibody (Neo Markers, Fremont, Calif., USA), anti-human TGFA monoclonal antibody (Calbiochem, Darmstadt, Germany), anti-human ADAM9 monoclonal antibody (R&amp;D Systems Inc. Minneapolis, Minn., USA), anti-human CD9 monoclonal antibody (Novocastra Laboratories Ltd, Newcastle upon Tyne, UK), or anti-human OSMR monoclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, Calif., USA), was added, and then HRP-labeled anti-rabbit or anti-mouse IgG as the secondary antibody. Substrate-chromogen was then added and the specimens were counterstained with hematoxylin.  
         [0106]     Frozen tissue samples from 11 patients were selected for analysis of immunohistochemistry. Positivity of immunostaining was assessed semi-quantitatively by scoring intensity as absent or positive by three independent investigators without prior knowledge of the clinical follow-up data. Cases were accepted only as positive if reviewers independently defined them thus.  
         [0000]     ELISA  
         [0107]     Serum was obtained from an independent set of 35 lung-ADC patients who were treated with gefitinib based on the same protocol as this clinical study at Hiroshima University hospital in Japan (5 for PR, 10 for SD, and 20 for PD). The sera of all the patients were obtained with informed consent at the time of diagnosis and every 4 weeks after the beginning of treatment, and stored at −80° C. The serum TGFA levels were measured by an ELISA using a commercially available enzyme test kits (TGF-alpha ELISA kit: Oncogene Rsearch Products, San Diego, Calif., USA).  
         [0000]     In Vitro Gefitinib Treatment and AREG-Autocrine Assay  
         [0108]     Human NSCLC (adenocarcinoma) cell lines PC-9, NCI-H358, and NCI-H522 were purchased from the American Type Culture Collection (ATCC; Rockville, Md., USA). To detect expression of AREG in these NSCLC cells, total RNA from each line was reverse-transcribed for single-stranded cDNAs using oligo(dT) 12-18  primer and Superscript II (Invitrogen). Semi-quantitative reverse transcriptase-PCR (RT-PCR) was carried out as described previously. 14  gefitinib (4-(3-chloro-4-fluoroanilino)-7-methoxy-6-(3-morpholinopropoxy)quinazoline: ZD 1839, Iressa), an inhibitor of epidermal growth factor receptor tyrosine kinase, was provided by AstraZeneca Pharmaceuticals (Macclesfield, UK). The drug was dissolved in DMSO at a concentration of 10 mM and kept at −20° C.  
         [0109]     We performed flow-cytometry to determine the sensitivity of lung adenocarcinoma cell lines to gefitinib treatment. Cells were plated at densities of 5×10 cells/100-mm dish and treated with 1·0 μM of gefitinib in appropriate serum-free medium. The cells were trypsinized 72 hours after the treatment, collected in PBS, and fixed in 70% cold ethanol for 30 min. After treatment with 100 μg/ml RNase (Sigma-Aldrich Co., St. Louis, Mo., USA), the cells were stained with 50 μg/ml propidium iodide (Sigma-Aldrich Co.) in PBS. Flow cytometry was performed on a Becton Dickinson FACScan and analyzed by ModFit software (Verity Software House, Inc., Topsham, Me., USA). The percentages of nuclei in G0/G1, S, and G2/M phases of the cell cycle and sub-G1 population were determined from at least 20,000 ungated cells.  
         [0110]     To investigate whether AREG functions as an autocrine anti-apoptotic factor in lung adenocarcinoma cells treated with gefitinib, we carried out the following assay. First, gefitinib-sensitive PC-9 cells, which do not express AREG, were cultured in serum-free medium for at least 8 hours prior to gefitinib treatment. These cells were then incubated with 0·5 or 1·0 μM of gefitinib for 72 hours in media that were either serum-free or supplemented with 10% FCS, or in serum-free conditioned medium collected from 72-hour cultures of AREG-expressing cells (NCI-H358 or NCI-H522). Each medium was replaced once with the same medium containing gefitinib at the 48-hour time point. To detect the response of each cell line to gefitinib, viability was evaluated by MTT assays using Cell Counting Kits (WAKO, Osaka, Japan).  
         [0111]     To confirm the autocrine effect of AREG on the gefitinib-resistance of NSCLC cells, we cultured PC-9 cells for 72 hours in serum-free medium containing 1·0 μM of gefitinib and recombinant AREG protein (Genzyme-Techne, Minneapolis, Minn., USA) in final concentrations of 1-100 ng/ml. Cell viability was evaluated by MTT assays. A possible effect of AREG itself on the viability of NSCLC cells was evaluated also, by culturing the PC-9 cells in serum- and gefitinib-free medium containing only recombinant AREG protein. MTT assays were performed as above.  
         [0000]     Results  
         [0000]     Response to Gefitinib Treatment  
         [0112]     Of the 53 patients enrolled in this trial, 46 had tumors diagnosed as adenocarcinomas (86·8%); five were squamous-cell carcinomas (9·4%); two were large cell carcinomas (3·8%). Fifteen patients achieved a PR and nobody revealed a CR; 17 patients were classified as SD, and 19 as PD. No clinical-response data were available for two of the patients. The tumor-response rate (CR+PR/CR+PR+SD+PD) for this treatment was 29·4%, and the disease control rate (CR+PR+SD/CR+PR+SD+PD) was 62·8% (table 1).  
         [0113]     Tumor samples were collected from 43 patients. Samples from 32 of those 43 contained sufficient numbers of cancer cells for analysis of expression profiles on our cDNA microarray. The numbers of samples that were judged to be suitable for further microarray analysis, were 8 for PR, 7 for SD, and 13 for PD (table 2). 17 of the 28 samples were analyzed as learning cases (7 for PR and 10 for PD), and 11 were as test cases (1 for PR, 3 for PD, and 7 for SD) for establishing a predictive scoring system for the efficacy of gefitinib treatment. For further validation of the prediction system, another blinded set of samples from 5 newly enrolled test-cases (4 for PD and 1 for SD) were obtained and added finally to the initial 11 test cases above.  
         [0000]     Identification of Genes Associated with Sensitivity to Gefitinib  
         [0114]     We attempted to extract genes that were differentially expressed between tumors from seven patients in the PR group (defined as responders) and those from 10 patients in the PD group (defined as non-responders) by comparing expression levels of 27,648 genes. (tables 2, 3).  
         [0115]     We carried out a random-permutation test to distinguish between the two subclasses defined by tumor response, and identified 51 genes whose permutational p-values were less than 0·001 (table 4). Expression levels of 40 genes were higher, and those of the other 11 were lower, in the non-responders.  
         [0116]     Establishment of a predictive scoring system for the efficacy of gefitinib treatment Based on the expression profiles of the 51 genes selected above, we tried to establish a predictive scoring system for the efficacy of gefitinib treatment. Prediction scores, termed gefitinib response score (GRS), were calculated according to procedures described previously (see Methods). To determine the number of candidates that provided the best separation of the two groups, we ranked the 51 genes on the basis of the significance of their permutational p-values and calculated prediction scores by the leave-one-out test, in decrements of 1 starting from the bottom of the rank-ordered list (51, 50, 49, 48 etc.). We calculated a classification score (CS), a standard we had previously defined for evaluation of the ability to discriminate two classes, for each set of genes. 17    
         [0117]     As shown in  FIG. 2A , we obtained different prediction scores when the number of discriminating genes was changed. We obtained the best CS, meaning the best separation of responders from non-responders, when we calculated the scores using only the 12 top-ranked genes in our candidate list.  
         [0118]     Hierarchical clustering analyses using all 51 genes, or only the top 12, classified all 17 cases into one of two groups according to the response to gefitinib ( FIG. 2 , B). The two groups were most clearly separated when we used the top 12 genes for cluster analysis. Finally, we established a numerical drug-response-scoring algorithm that might be clinically applicable for predicting sensitivity of an individual NSCLC to gefitinib, on the basis of expression levels of the 12 selected genes.  
         [0119]     To validate this prediction system we investigated 8 additional (“test”) NSCLC cases (1 for PR and 7 for PD) that were completely independent of the 17 “learning” cases used for establishing the system. We examined gene-expression profiles in each of those samples and then calculated GRS on the basis of the expression levels of the 12 discriminating genes. As shown in  FIG. 2C , scores obtained by the GRS system were concordant with the clinical responses to gefitinib in all eight “test” cases.  
         [0000]     GRS Values for Patients with SD in Tumor Response  
         [0120]     GRS values for the eight test-SD patients were calculated according to the predictive scoring system established above. Although the values were widely distributed from −83·0 (predicted as non-responder) to 61·6 (responder), the scores of patients who retained SD status throughout the observation period were likely to be higher than those of patients who had been judged as SD at a certain time-point of the study but showed progression of the disease within three or four months after the start of treatment ( FIG. 2 , C). Although the GRS system was established on the basis of gene-expression profiles that distinguished between patients with PR and patients with PD (without SD) in tumor response, these results suggest that the GRS serves in classifying SD patients into groups according to their response to gefitinib.  
         [0000]     Validation of GRS with Semi-Quantitative RT-PCR Analysis  
         [0121]     To confirm differential expression of the top 12 predictive genes between PR and PD cases, expression values derived from microarray data were correlated with values from semi-quantitative RT-PCR of RNAs from the same patients (5 PR and 7 PD) ( FIG. 3 , A, table 5, A). Spearman rank correlations were positive for all of the 12 genes and significantly positive for seven of 12 genes.  
         [0000]     Immunohistochemical Validation of GRS  
         [0122]     To validate differential expression of the predictive protein markers between PR and PD cases, we carried out immunohistochemical staining with five different antibodies for AREG, TGFA, ADAM9, CD9, and OSMR, all of which were known to be involved in the ligand-EGFRs signalling and whose permutational p-values were less than 0·01. We first stained paired tumor tissue sections obtained by TBB and lymph-node biopsy from the same patients using these 5 antibodies. No intra-patient differences on protein expression of these five markers were observed in three different patients ( FIG. 3 , B). We also validated the microarray data with the five markers in 11 NSCLC samples (5 for PR and 6 for PD). The results were consistent with the microarray data ( FIG. 3 , C, table 5, B).  
         [0000]     Serum Levels of TGFA  
         [0123]     To further evaluate the availability of the prediction system in routine clinical situations, we detected TGFA protein using ELISA in serum samples from 5 PR, 10 SD, and 20 PD patients that were independently collected for serological test and were not enrolled in microarray analysis. The serum levels of TGFA were 19·0±2·8 pg/ml (mean±SE) in PD patients, 13·9±1·9 pg/ml in SD patients, and 12·8±1·4 pg/ml in PR patients ( FIG. 4 ). Twelve of 20 serum samples from PD patients were positive for TGFA and all samples from PR patients were negative, when 16·0 pg/ml was used as a cutoff.  
         [0000]     In Vitro Gefitinib Treatment and AREG-Autocrine Assay  
         [0124]     AREG, a ligand for EGFR and other ERBB members was significantly over-expressed in non-responders but not (or hardly) detectable in responders. To investigate whether AREG protein leads to resistance of NSCLCs to gefitinib therapy when it is secreted in an autocrine manner, we performed the following biological analyses. We initially identified expression of AREG mRNA in lung-adenocarcinoma cell lines NCI-H358 and -H522, but not in PC-9, by means of RT-PCR experiments ( FIG. 5 , A). Next, we performed flow-cytometric analysis 72 hours after treatment of PC-9 cells with 1·0 μM of gefitinib, and found that gefitinib increased the percentages of nuclei in sub-G1 (24%) compared with cells with no treatment (6%) (data not shown). This result suggested that gefitinib might induce apoptosis in PC-9 cells.  
         [0125]     We then analyzed the viability of PC-9 cells, which are gefitinib-sensitive and do not express AREG, after culture in serum-free medium or in serum-free, conditioned medium obtained from NCI-H358 or -H522 cells grown in the presence or absence of 0·5 or 1·0 μM of gefitinib. As shown in  FIG. 5B , the viability of PC-9 cells incubated in the serum-free, conditioned medium containing gefitinib was greater than that of PC-9 cells grown in serum-free medium with the same concentrations of gefitinib. As the supplier of gefinitib has reported previously, the anti-tumor effect of gefitinib decreases in the presence of 10% FCS, suggesting that this assay should be suitable for quantitative measurement of gefitinib dosage and activity.  
         [0126]     To investigate whether AREG, secreted in an autocrine manner, inhibits apoptosis of NSCLC cells treated with gefitinib, we cultured PC-9 cells in serum-free medium containing recombinant AREG protein at final concentrations of 1-100 ng/ml, in the presence or absence of 1·0 μM gefitinib. The viability of PC-9 cells incubated with both AREG and 1·0 μM gefitinib was increased in comparison to cells incubated with 1·0 μM gefitinib only, in an AREG-dose-dependent manner ( FIG. 5 , C). On the other hand, recombinant AREG alone had no effect on the viability of PC-9 cells ( FIG. 5 , C). This observation appeared to indicate that AREG inhibits the apoptosis induced by gefitinib, but does not in itself affect cell viability. Immunostaining for AREG is shown in  FIG. 6 .  
         [0000]     Discussion  
         [0127]     A large body of evidence supports the view that molecules in the EGFR autocrine pathway are involved in a number of processes important to cancer formation and progression, including cell proliferation, angiogenesis, and metastatic spread. 5  Therapeutic blockade of specific signalling, therefore, could be a promising strategy for cancer treatment. Gefitinib, a synthetic anilinoquinazoline, inhibits the tyrosine kinase activity of EGFR by competing with adenosine triphosphate for a binding site on the intracellular domain of the receptor. 7  In phase II trials (IDEAL 1 and IDEAL 2), use of gefitinib as a 2nd-, 3rd-, or 4th-line monotherapy for advanced NSCLC achieved tumor-response rates of nearly 20%, 8-10  which were superior to those achieved with conventional cytotoxic agents. Multivariate analysis of patients in the IDEAL 1 study suggested that the response rate in females might be higher than in males, and higher in patients with adenocarcinomas than in patients with squamous-cell carcinomas (odds ratios 2·7 and 3·5 respectively). 9  Recent study suggested that individuals in whom gefitinib is efficacious are more likely to have adenocarcinomas of the bronchioloalveolar subtype and to be never smokers (odds ratios 13·5 and 4·2 respectively). 19  The higher tumor-response rate (29·4%) documented in the clinical trial reported here might reflect a higher proportion of patients with adenocarcinoma (46 adenocarcinomas, five squamous-cell carcinomas and two large-cell carcinomas) than has been the case in other studies. The clinicopathological determinants of gefitinib sensitivity including bronchioloalveolar carcinoma (BAC) features are predictve to a certan extent, 9,10,19,20  however, previous reports and our observations obviously suggest that no factors can perfectly predict the response of NSCLC to gefitinib treatment. Therefore novel methods to discriminate responders from non-responders in advance could allow a more focused use of gefitinib in clinical settings.  
         [0128]     By statistical analysis of gene-expression profiles of advanced NSCLCs obtained on cDNA microarrays, we identified dozens of genes associated with sensitivity to gefitinib. We introduced a prediction-scoring system based on expression of the 12 genes that had shown the most significant differences in expression levels between responder (PR) and non-responder (PD) groups. This set of genes was selected from expression profiles of lung adenocarcinomas; however, the GRS system successfully classified all eight of our “test” PR and PD cases in accord with their clinical responses to gefitinib, and one of them was a squamous-cell carcinoma. Moreover, this system was likely to separate intermediate tumor responses (SD) into two groups, one representing patients who succeeded in maintaining the tumor-static effect for a long period and the other representing patients who failed to do so.  
         [0129]     In practical terms, we need to predict the chemosensitivity of individual tumors using the minimally invasive techniques available at every hospital, because patients with advanced NSCLCs are rarely candidates for surgical resection of their tumors. Therefore we have tried to establish a prediction system that requires only the amount of cancerous tissue that can be obtained by, for example, flexible bronchofiberscopy. By verifying individual steps of the method, we were able to precisely profile gene expression in biopsy specimens as small as 1 mm. Relevant microarray results were confirmed by semi-quantitative RT-PCR for 12 genes that showed the most significant differences to establish a GRS system. Furthermore, we validated the effectiveness of antibodies for 5 different biomarkers (AREG, TGFA, ADAM9, CD9, and OSMR), all of which were reported to be involved in the ligand-EGFR signalling, for discriminating potential responders from non-responders, in both TBB and lymph-node biopsy samples. Moreover, we were able to detect serum TGFA proteins in lung-ADC patients by ELISA. Further evaluation of these markers for clinical use are necessary, however, the limited number of genes required for prediction should eventually enable laboratories to diagnose in advance the efficacy of gefitinib treatment for an NSCLC patient, using routine procedures such as serological examinations of blood, PCR experiments, or immunohistochemical analysis of biopsy specimens.  
         [0130]     To our knowledge, this is the first report about gene-expression profiles of unresectable “advanced” lung cancers, although profiles of surgically resected specimens of “early” lung cancers have been reported. 21,22  However, about 70% of tumors in patients diagnosed with NCSLC are already locally advanced or metastatic, which generally renders them resistant to conventional therapeutic modalities. Therefore the genes listed here should be useful for disclosing molecular mechanisms of lung-cancer progression and may be potential targets for drug development.  
         [0131]     Gefitinib was developed as a “selective” inhibitor of EGFR-TK; however, no clear association between the level of EGFR activation and response to gefitinib has been found in vitro or in vivo. 7,23  In clinical trials, gefitinib has been more effective against adenocarcinomas than against squamous-cell carcinomas, 9,10  although over-expression of EGFR is less frequent in adenocarcinomas. 24  Therefore, it is important to identify which individual tumors are good targets for this treatment. In our analysis using clinical samples, the difference in EGFR protein expression between responders and non-responders were not statistically significant. On the other hand, amphiregulin (AREG) and transforming growth factor alpha (TGFA), both of which encode the ligand for EGFR and other ERBB members, were significantly over-expressed in non-responders but not (or hardly) detectable in responders p=0·0000000000093 and 0·0095 respectively; table 4).  
         [0132]     The significance of the ligands and the EGFR autocrine loop in growth and survival of lung-cancer cells is indisputable, 24-26  but the role of AREG in formation and progression of cancers is poorly understood. However, several lines of evidence suggest that over-expression of AREG is associated with shortened survival of patients with NSCLC. 24  Moreover, anti-apoptotic activity of AREG in human lung-adenocarcinoma cells was reported recently. 25  To investigate whether the anti-apoptotic activity of AREG leads to resistance of NSCLC cells to gefitinib therapy, we performed a biological assay using a gefitinib-sensitive but AREG-non-expressing NSCLC cell line, PC-9. We found that the anti-tumor activity of gefitinib on PC-9 cells was dramatically decreased by autocrine secretion of AREG. This evidence strongly suggests that although growth-factor signalling by the EGFR is markedly complicated at every step because of the multiplicity of ligands, dimerization partners, effectors, and downstream pathways, 26  AREG might be a principal activator of the ligands-receptor autocrine growth pathway that leads to cancer progression and resistance to gefitinib.  
         [0133]     Several elements associated with the EGFR-TK pathway are present on our list of differentially-expressed genes. For example, genes encoding dual specificity phosphatase 3 (DUSP3), ADAM9, CD9, and OSMR were expressed predominantly in non-responders (p=0·00000000094, 0·01, 0·000022, and 0.0000011, respectively). DUSP3 gene modulates EGFR signalling by dephosphorylating mitogen activated protein kinase (MAPK), a key mediator of signal transduction, 27  and ADAM9 is involved in activation of EGFR signalling by shedding the ectodomain of proHB-EGF (pro Heparin-binding epidermal growth factor-like growth factor). 28  CD9 physically interacts with transmembrane TGFA. CD9 expression strongly decreases the growth factor- and PMA-induced proteolytic conversions of transmembrane to soluble TGFA and strongly enhances the TGFA-induced EGFR activation. 29  OSMR is reported to be constitutively associated with ERBB2 in breast cancer cells. 30  Although other target molecules for gefitinib have been suggested, our results suggest that EGFR signalling is at least one of the important processes involved in response to this drug.  
         [0134]     Since gefitinib can induce apoptosis of some cancer cells in vivo, other molecules with anti-apoptotic activity, as well as AREG, may contribute to a tumor&#39;s resistance to the drug. AVEN (apoptosis, caspase-activation inhibitor), which was specifically expressed in our non-responders (p=0·00000000042), is known to enhance the anti-apoptotic activity of Bcl-xL and to suppress Apaf-1-mediated caspase activation. 31  On the other hand, mechanisms regulating drug transport should also affect drug resistance. GCLC (glutamate-cysteine ligase, catalytic subunit), which plays an important role in cellular detoxification of anticancer drugs such as cisplatin, etoposide and doxorubicin, 32  was over-expressed in our group of non-responders (p=0·00000012). As these genes correlated negatively with responses to chemotherapy in our panel of tumors (i.e. the higher the expression of these genes, the greater the resistance to gefitinib), they might be involved in the mechanism(s) leading to that resistance. It should be noted also that the functions of nearly half of our candidate prediction-genes are unknown. Therefore further investigations will be needed to reveal more clearly the biological events underlying responses of NSCLCs to gefitinib.  
         [0135]     In summary, we identified 51 genes whose expression differed significantly between responders and non-responders to gefitinib among human lung carcinomas, and established a numerical scoring system, based on expression patterns of 12 of those genes, to predict the response of individual tumors to this drug. Although further validation using a larger set of clinical cases will be necessary, the data presented here may yield valuable insights into the molecular events underlying signal-suppressing strategies and provide important information about gefitinib treatment for individual NSCLC patients by testing a set of genes with high predictive values.  
       REFERENCES  
       [0136]     1. Fossella, F. V., et al.,  Randomized phase III trial of docetaxel versus vinorelbine or ifosfamide in patients with advanced non - small - cell lung cancer previously treated with platinum - containing chemotherapy regimens. The TAX  320  Non - Small Cell Lung Cancer Study Group.  J Clin Oncol, 2000. 18(12): p. 2354-62.  
         [0137]     2.  Non - small Cell Lung Cancer Collaborative Group. Chemotherapy in non - small cell lung cancer: a meta - analysis using updated data on individual patients from  52  randomised clinical trials.  Bmj, 1995. 311(7010): p. 899-909.  
         [0138]     3. Schiller, J. H., et al.,  Comparison of four chemotherapy regimens for advanced non - small - cell lung cancer.  N Engl J Med, 2002. 346(2): p. 92-8.  
         [0139]     4. Kelly, K., et al.,  Randomized phase III trial of paclitaxel plus carboplatin versus vinorelbine plus cisplatin in the treatment of patients with advanced non - small - cell lung cancer: a Southwest Oncology Group trial.  J Clin Oncol, 2001. 19(13): p. 3210-8.  
         [0140]     5. Baselga, J.,  Why the epidermal growth factor receptor? The rationale for cancer therapy.  Oncologist, 2002. 7 Suppl 4: p. 2-8.  
         [0141]     6. Traxler, P.,  Tyrosine kinases as targets in cancer therapy—successes and failures.  Expert Opin Ther Targets, 2003. 7(2): p. 215-34.  
         [0142]     7. Wakeling, A. E., et al.,  ZD 1839 ( Iressa ):  an orally active inhibitor of epidermal growth factor signaling with potential for cancer therapy.  Cancer Res, 2002. 62(20): p. 5749-54.  
         [0143]     8. Herbst, R. S.,  Dose - comparative monotherapy trials of ZD 1839  in previously treated non - small cell lung cancer patients.  Semin Oncol, 2003. 30(1 Suppl 1): p. 30-8.  
         [0144]     9. Fukuoka, M., et al.,  Final results from a phase □ trial of ZD 1839 (‘ Iressa ’)  for patients with advanced non - small cell lung cancer  ( IDEAL  1). Pro Am Soc Clin Oncol 2002. 21;298a(A1188).  
         [0145]     10. Kris, M G., et al.,  A phase II trial of ZD 1839 (‘ Iressa ’)  in advanced non - small cell lung cancer  ( NSCLC )  patients who had failed platinum - and docetaxel - based regimens  ( IDEAL  2). Pro Am Soc Clin Oncol 2002. 21;292a(A1166).  
         [0146]     11. Inoue, A., et al.,  Severe acute interstitial pneumonia and gefitinib.  Lancet, 2003. 361(9352): p. 137-9.  
         [0147]     12. Bohm, M., et al.,  Microbeam MOMeNT: non - contact laser microdissection of membrane - mounted native tissue.  Am J Pathol, 1997. 151(1): p. 63-7.  
         [0148]     13. Okabe, H., et al.,  Genome - wide analysis of gene expression in human hepatocellular carcinomas using cDNA microarray: identification of genes involved in viral carcinogenesis and tumor progression.  Cancer Res, 2001. 61(5): p. 2129-37.  
         [0149]     14. Kitahara, O., et al.,  Alterations of gene expression during colorectal carcinogenesis revealed by cDNA microarrays after laser - capture microdissection of tumor tissues and normal epithelia.  Cancer Res, 2001. 61(9): p. 3544-9.  
         [0150]     15. Golub, T. R., et al.,  Molecular classification of cancer: class discovery and class prediction by gene expression monitoring.  Science, 1999. 286(5439): p. 531-7.  
         [0151]     16. MacDonald, T. J., et al.,  Expression profiling of medulloblastoma: PDGFRA and the RAS/MAPK pathway as therapeutic targets for metastatic disease.  Nat Genet, 2001. 29(2): p. 143-52.  
         [0152]     17. Kaneta. Y.,et al.,  Prediction of sensitivity to STI 571  among chronic myeloid leukemia patients by genome - wide cDNA microarray analysis.  Jpn J Cancer Res 2002. 93, p. 849-856.  
         [0153]     18. Pavelic, K., et al.,  Evidence for a role of EGF receptor in the progression of human lung carcinoma.  Anticancer Res, 1993. 13(4): p. 1133-7.  
         [0154]     19. Kikuchi, T., et al.,  Expression profiles of non - small cell lung cancers on cDNA microarrays: Identification of genes for prediction of lymph - node metastasis and sensitivity to anti - cancer drugs.  Oncogene, 2003. 22(14): p. 2192-205.  
         [0155]     20. Heighway, J., et al.,  Expression profiling of primary non - small cell lung cancer for target identification.  Oncogene, 2002. 21(50): p. 7749-63.  
         [0156]     21. Beer, D. G., et al.,  Gene - expression profiles predict survival of patients with lung adenocarcinoma.  Nat Med, 2002. 8(8): p. 816-24.  
         [0157]     22. Miura, K., et al.,  Laser capture microdissection and microarray expression analysis of lung adenocarcinoma reveals tobacco smoking - and prognosis - related molecular profiles.  Cancer Res, 2002. 62(11): p. 3244-50.  
         [0158]     23. Moasser, M. M., et al.,  The tyrosine kinase inhibitor ZD 1839 (“ Iressa ”)  inhibits HER 2- driven signaling and suppresses the growth of HER 2- overexpressing tumor cells.  Cancer Res, 2001. 61(19): p. 7184-8.  
         [0159]     24. Rusch, V., et al.,  Overexpression of the epidermal growth factor receptor and its ligand transforming growth factor alpha is frequent in resectable non - small cell lung cancer but does not predict tumor progression.  Clin Cancer Res, 1997. 3(4): p. 515-22.  
         [0160]     25. Fontanini, G., et al.,  Evaluation of epidermal growth factor - related growth factors and receptors and of neoangiogenesis in completely resected stage I - IIIA non - small - cell lung cancer: amphiregulin and microvessel count are independent prognostic indicators of survival.  Clin Cancer Res, 1998. 4(1): p. 241-9.  
         [0161]     26. Brundage, M. D., D. Davies, and W. J. Mackillop,  Prognostic factors in non - small cell lung cancer: a decade of progress.  Chest, 2002. 122(3): p. 1037-57.  
         [0162]     27. Hurbin, A., et al.,  Inhibition of apoptosis by amphiregulin via an insulin - like growth factor -1  receptor - dependent pathway in non - small cell lung cancer cell lines.  J Biol Chem, 2002. 277(51): p. 49127-33.  
         [0163]     28. Yarden, Y. and M. X. Sliwkowski,  Untangling the ErbB signalling network.  Nat Rev Mol Cell Biol, 2001. 2(2): p. 127-37.  
         [0164]     29. Prenzel, N., et al.,  EGF receptor transactivation by G - protein - coupled receptors requires metalloproteinase cleavage of proHB-EGF.  Nature 1999. 402(6764):884-8  
         [0165]     30. Nelson, Chau, et al.,  Aven, a novel inhibitor of caspase activation, binds Bcl - xL and Apaf -1. Molec Cell 2000.6: p. 31-41.  
         [0166]     31. Tipnis, S R., et al.,  Overexpression of the regulatory subunit of r - glutamylcysteine synthetase in Hela cells increases r - glutamylcysteine synthetase activity and confers drug resistance.  Biochem J 1999. 337, p. 559-566.  
                                           TABLE 1                           Summary of baseline patient characteristics and response            Characteristics   Percentage (%)   Number of Patient                    Sex               male   58.5   (31)       female   41.5   (22)       Age       median   59       range   35-80       Histology       adenocarcinoma   86.8   (46)       squamous cell carcinoma   9.4    (5)       large cell carcinoma   3.8    (2)       Stage       IIIA   1.9    (1)       IIIB   7.5    (4)       IV   90.6   (48)       Performance Status       0   26.4   (14)       1   60.4   (32)       2   13.2    (7)       Number of Prior Regimen       1   24.5   (13)       2   35.9   (19)       3   28.3   (15)       4   0    (0)       5   7.5    (4)       6   3.8    (2)       Response to Gefitinib Therapy       CR   0    (0)       PR   28.3   (15)       SD   32.1   (17)       PD   35.8   (19)       unknown   3.8    (2)       Tumor Response Rate (             29.4   (15)       (CR + PR/CR + PR + SD + PD)       Disease Control Rate (%)   62.8   (32)       (CR + PR + SD/CR + PR +       SD + PD)                  
 
         [0167]    
       
         
               
             
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                   
               
               
                 Number of cases suitable for analysis and their best overall responses 
               
             
          
           
               
                   
                 Best Overall Response 
               
             
          
           
               
                 Number of Cases 
                 PR 
                 SD 
                 PD 
                 Unknown 
                 Total 
               
               
                   
               
             
          
           
               
                 All cases enrolled 
                 15 
                 17 
                 19 
                 2 
                 53 
               
               
                 Cases that consented to the 
                 15 
                 14 
                 13 
                 1 
                 43 
               
               
                 study 
               
               
                 Cases suitable for analysis 
                 8 
                 10 
                 13 
                 1 
                 32 
               
               
                 Learning cases (1) 
                 7 
                 0 
                 10 
                 0 
                 17 
               
               
                 Test cases (1,2) 
                 1 
                 7 
                 3 
                 0 
                 11 
               
               
                   
               
               
                   (1) Learning cases were used for developing the GRS, whereas test cases were used for validation of the    
               
               
                   (2) Another blinded set of samples from 5 newly enrolled cases (4 PD and 1 SD) were also added to these 11 test cases later.    
               
             
          
         
       
     
         [0168]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 3 
               
               
                   
               
               
                   
               
               
                 Clinicopathological features of patients 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Number of 
                 EGFR Stained 
                   
               
             
          
           
               
                 Case 
                   
                   
                 Histology 
                   
                   
                   
                 Stage 
                 Previous 
                 Tumour Cell 
                 EGFR 
               
               
                 No. (*) 
                 Sex 
                 Age 
                 Type (1) 
                 T 
                 N 
                 M 
                 Classification (2) 
                 Chemotherapy 
                 (%) 
                 mutation (3) 
               
               
                   
               
               
                 LC01 
                 female 
                 36 
                 ADC 
                 1 
                 0 
                 1 
                 IV 
                 1 
                   
                 None detected 
               
               
                 LC02 
                 male 
                 64 
                 ADC 
                 2 
                 3 
                 1 
                 IV 
                 3 
                 80 
                   
               
               
                 LC03 
                 female 
                 54 
                 ADC 
                 2 
                 0 
                 1 
                 IV 
                 3 
                 80 
                   
               
               
                 LC04 
                 female 
                 75 
                 ADC 
                 2 
                 1 
                 1 
                 IV 
                 1 
                 20 
                 None detected 
               
               
                 LC05 
                 female 
                 73 
                 ADC 
                 0 
                 2 
                 1 
                 IV 
                 5 
                 30 
                 46 A750del 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 (2481 2495           
               
               
                 LC06 
                 female 
                 75 
                 ADC 
                 4 
                 1 
                 1 
                 IV 
                 3 
                   
                 None detected 
               
               
                 LC07 
                 female 
                 70 
                 ADC 
                 2 
                 1 
                 1 
                 IV 
                 3 
                 80 
                 47_A750del 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 (2485_2496d 
               
               
                 LC08 
                 female 
                 47 
                 ADC 
                 4 
                 3 
                 1 
                 IV 
                 2 
                 95 
                 L858R 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 (2819T &gt; G) 
               
             
          
           
               
                 mean (range) 
                 62 
                   
                   
                   
                   
                   
                 2.6 (1-5) 
                 64 (20-95) 
                   
               
               
                   
                 (36-75) 
                   
                   
                   
                   
                   
                   
                   
                   
               
             
          
           
               
                 LC09 
                 female 
                 63 
                 ADC 
                 4 
                 0 
                 1 
                 IV 
                 3 
                 90 
                   
               
               
                 LC10 
                 male 
                 56 
                 ADC 
                 2 
                 0 
                 1 
                 IV 
                 6 
                 70 
                   
               
               
                 LC11 
                 male 
                 67 
                 ADC 
                 4 
                 0 
                 1 
                 IV 
                 2 
                 0 
                   
               
               
                 LC12 
                 male 
                 53 
                 ADC 
                 4 
                 3 
                 1 
                 IV 
                 2 
                   
                 None detected 
               
               
                 LC13 
                 female 
                 56 
                 ADC 
                 4 
                 2 
                 0 
                 IIIB 
                 2 
                 40 
                   
               
               
                 LC14 
                 female 
                 62 
                 ADC 
                 4 
                 2 
                 1 
                 IV 
                 3 
                 60 
                   
               
               
                 LC15 
                 male 
                 61 
                 ADC 
                 0 
                 0 
                 1 
                 IV 
                 5 
                 60 
                   
               
             
          
           
               
                 mean (range) 
                 60 
                   
                   
                   
                   
                   
                 3.3 (2-6) 
                 53 (0-90) 
                   
               
               
                   
                 (53-67) 
                   
                   
                   
                   
                   
                   
                   
                   
               
             
          
           
               
                 LC16 
                 male 
                 42 
                 ADC 
                 4 
                 3 
                 1 
                 IV 
                 5 
                 90 
                 None detected 
               
               
                 LC17 
                 female 
                 54 
                 ADC 
                 2 
                 3 
                 1 
                 IV 
                 2 
                 50 
                 None detected 
               
               
                 LC18 
                 female 
                 61 
                 ADC 
                 1 
                 3 
                 0 
                 IIIB 
                 2 
                   
                 None detected 
               
               
                 LC19 
                 male 
                 59 
                 ADC 
                 0 
                 2 
                 1 
                 IV 
                 2 
                 30 
                 W817C 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 (2697G &gt; T) 
               
               
                 LC20 
                 male 
                 65 
                 ADC 
                 0 
                 3 
                 1 
                 IV 
                 3 
                   
                 None detected 
               
               
                 LC21 
                 male 
                 55 
                 ADC 
                 4 
                 3 
                 1 
                 IV 
                 3 
                 80 
                 None detected 
               
               
                 LC22 
                 male 
                 80 
                 ADC 
                 4 
                 3 
                 1 
                 IV 
                 2 
                 80 
                 Q787Q 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 (2607G &gt; A) 
               
               
                 LC23 
                 male 
                 35 
                 ADC 
                 4 
                 0 
                 1 
                 IV 
                 5 
                   
                 None detected 
               
               
                 LC24 
                 male 
                 57 
                 ADC 
                 4 
                 3 
                 1 
                 IV 
                 1 
                  0 
                 None detected 
               
               
                 LC25 
                 female 
                 65 
                 ADC 
                 2 
                 0 
                 1 
                 IV 
                 1 
                   
                 None detected 
               
               
                 LC26 
                 male 
                 64 
                 SCC 
                 3 
                 3 
                 1 
                 IV 
                 2 
                   
                 None detected 
               
               
                 LC27 
                 female 
                 65 
                 ADC 
                 4 
                 2 
                 1 
                 IV 
                 1 
                   
                 L858R 
               
               
                   
                   
                   
                   
                   
                   
                   
                   
                   
                   
                 (2819T &gt; G) 
               
               
                 LC28 
                 male 
                 74 
                 ADC 
                 2 
                 1 
                 1 
                 IV 
                 1 
                 10 
                   
               
             
          
           
               
                 mean (range) 
                 60 
                   
                   
                   
                   
                   
                 2.3 (1-5) 
                 49 (0-90) 
                   
               
               
                   
                 (35-80) 
               
               
                   
               
             
          
           
               
                   
                 Plasma Gefitinib 
                 Response to Gefitinib(4) 
                   
                 Use for 
                   
               
             
          
           
               
                 Case 
                   
                   
                 Concentration 
                 1st 
                 2nd 
                 3rd 
                 4th 
                 Best Overall 
                 Prediction 
                   
               
               
                 No. (*) 
                 Sex 
                 Age 
                 (ng/ml) 
                 month 
                 month 
                 month 
                 month 
                 Response (5) 
                 (6) 
                 GRS (7) 
               
               
                   
               
             
          
           
               
                 LC01 
                 female 
                 36 
                 258.9 
                 PR 
                 PR 
                 PR 
                 PR 
                 PR 
                 learning 
                 100 
               
               
                 LC02 
                 male 
                 64 
                 140.3 
                 PR 
                 PR 
                 PR 
                 PR 
                 PR 
                 learning 
                 100 
               
               
                 LC03 
                 female 
                 54 
                 167.0 
                 PR 
                 PR 
                 PR 
                 PR 
                 PR 
                 learning 
                 100 
               
               
                 LC04 
                 female 
                 75 
                 169.7 
                 PR 
                 PR 
                 PR 
                 PR 
                 PR 
                 learning 
                 100 
               
               
                 LC05 
                 female 
                 73 
                 300.6 
                 PR 
                 PR 
                 PR 
                 PR 
                 PR 
                 learning 
                 100 
               
               
                 LC06 
                 female 
                 75 
                 874.0 
                 SD 
                 PR 
                 PR 
                 PR 
                 PR 
                 learning 
                 100 
               
               
                 LC07 
                 female 
                 70 
                 460.8 
                 SD 
                 PR 
                 PR 
                 PR 
                 PR 
                 learning 
                 100 
               
               
                 LC08 
                 female 
                 47 
                 306.5 
                 PR 
                 PR 
                 PR 
                 PR 
                 PR 
                 test 
                 54.8 
               
             
          
           
               
                 mean (range) 
                 62 
                 334.7 
                   
               
               
                   
                 (36-75) 
                 (140.3-874.0) 
               
             
          
           
               
                 LC09 
                 female 
                 63 
                 743.4 
                 SD 
                 SD 
                 SD 
                 SD 
                 SD 
                 test 
                 61.6 
               
               
                 LC10 
                 male 
                 56 
                 511.8 
                 SD 
                 SD 
                 SD 
                 SD 
                 SD 
                 test 
                 −9.8 
               
               
                 LC11 
                 male 
                 67 
                 631.3 
                 SD 
                 SD 
                 SD 
                 SD 
                 SD 
                 test 
                 −5.3 
               
               
                 LC12 
                 male 
                 53 
                 306.1 
                 SD 
                 SD 
                 SD 
                 PD 
                 SD 
                 test 
                 −23.8 
               
               
                 LC13 
                 female 
                 56 
                 364.8 
                 SD 
                 SD 
                 PD 
                   
                 SD 
                 test 
                 −58.5 
               
               
                 LC14 
                 female 
                 62 
                 322.4 
                 SD 
                 SD 
                 PD 
                   
                 SD 
                 test 
                 −83 
               
               
                 LC15 
                 male 
                 61 
                 278.9 
                 SD 
                 SD 
                 PD 
                   
                 SD 
                 test 
                 −40.5 
               
             
          
           
               
                 mean (range) 
                 60 
                 451.2 
                   
               
               
                   
                 (53-67) 
                 (278.9-631.3) 
               
             
          
           
               
                 LC16 
                 male 
                 42 
                 212.6 
                 SD 
                 PD 
                   
                   
                 PD 
                 learning 
                 −63.9 
               
               
                 LC17 
                 female 
                 54 
                 320.6 
                 SD 
                 PD 
                   
                   
                 PD 
                 learning 
                 −86 
               
               
                 LC18 
                 female 
                 61 
                 229.3 
                 SD 
                 PD 
                   
                   
                 PD 
                 learning 
                 −67.8 
               
               
                 LC19 
                 male 
                 59 
                 150.7 
                 SD 
                 PD 
                   
                   
                 PD 
                 learning 
                 −57.1 
               
               
                 LC20 
                 male 
                 65 
                 167.8 
                 SD 
                 PD 
                   
                   
                 PD 
                 learning 
                 −59.1 
               
               
                 LC21 
                 male 
                 55 
                   
                 PD 
                   
                   
                   
                 PD 
                 learning 
                 −73.1 
               
               
                 LC22 
                 male 
                 80 
                   
                 PD 
                   
                   
                   
                 PD 
                 learning 
                 −55.5 
               
               
                 LC23 
                 male 
                 35 
                   
                 PD 
                   
                   
                   
                 PD 
                 learning 
                 −100 
               
               
                 LC24 
                 male 
                 57 
                   
                 PD 
                   
                   
                   
                 PD 
                 learning 
                 −46.7 
               
               
                 LC25 
                 female 
                 65 
                 356.3 
                 PD 
                   
                   
                   
                 PD 
                 learning 
                 −86.1 
               
               
                 LC26 
                 male 
                 64 
                 405.6 
                 SD 
                 PD 
                   
                   
                 PD 
                 test 
                 −67.7 
               
               
                 LC27 
                 female 
                 65 
                   
                 PD 
                   
                   
                   
                 PD 
                 test 
                 −69.4 
               
               
                 LC28 
                 male 
                 74 
                   
                 PD 
                   
                   
                   
                 PD 
                 test 
                 −64.8 
               
             
          
           
               
                 mean (range) 
                 60 
                 263.2 
                   
               
               
                   
                 (35-80) 
                 (150.7-405.6) 
               
               
                   
               
               
                   (1) ADC, adenocarcinoma; SCC, squamous-cell carcinoma.    
               
               
                   (2) TNM clinical classification and stage grouping were assessed based on the UICC/WHO classification.    
               
               
                   (3) Mutation at codon position 709-870 (from p-loop to activation loop) of EGFR (GenBank Accession No. NM005228).    
               
               
                   (4) Objective Tumor Response to Gefitinib was assessed every 4 weeks after the start of treatment using UICC/WHO Criteria. PR, partial response; SD, stable disease; PD, progressive disease    
               
               
                   (5) Overall Best Response was evaluated based on the definitions as mentioned in materials and methods.    
               
               
                   (6) learning, samples used for developing the GRS; test, samples used for validation of the GRS.    
               
               
                   (7) GRS: gefitinib response score determined by prediction system    
               
               
                   (*) For further validation of the GRS, another blinded set of samples from 5 newly enrolled cases (4 PD and 1 SD) were also added to these 28 cases later.    
               
             
          
         
       
     
         [0169]    
       
         
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                   
               
               
                 List of 51 candidate genes for discriminating responder (PR) from non-responder (PD) to gefitinib (*) 
               
             
          
           
               
                   
                   
                   
                   
                   
                   
                 Median-fold 
               
               
                 Rank 
                   
                   
                   
                 Predominantly 
                 Permutational 
                 Difference 
               
               
                 Or           
                 GenBank 
                 Symbol 
                 Gene Name 
                 Expressed Clas 
                 p-value 
                 (log2) 
               
               
                   
               
             
          
           
               
                 1 
                 NM_0248           
                 FLJ2266           
                 hypothetical protein FLJ22662 
                 PD 
                 8.1E−12 
                 2.0 
               
               
                 2 
                 BC009799 
                 AREG 
                 amphiregulin (schwannoma-derived growth factor) 
                 PD 
                 9.3E−12 
                 8.0 
               
               
                 3 
                 NM_0143           
                 CORO1C 
                 coronin, actin binding protein, 1C 
                 PD 
                 2.3E−10 
                 4.6 
               
               
                 4 
                 BC010488 
                 AVEN 
                 apoptosis, caspase activation inhibitor 
                 PD 
                 4.2E−10 
                 4.3 
               
               
                 5 
                 NM_0040           
                 DUSP3 
                 dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-rel           
                 PD 
                 9.4E−10 
                 4.4 
               
               
                 6 
                 AI026836 
                 DJ473B4 
                 hypothetical protein dJ473B4 
                 PD 
                 1.7E−09 
                 8.0 
               
               
                 7 
                 BU500509 
                 PHLDA2 
                 pleckstrin homology-like domain, family A, member 2 
                 PD 
                 1.8E−09 
                 8.0 
               
               
                 8 
                 NM_0160           
                 RBM7 
                 RNA binding motif protein 7 
                 PD 
                 1.8E−08 
                 2.9 
               
               
                 9 
                 BX092512 
                   
                 EST 
                 PD 
                 7.7E−08 
                 3.0 
               
               
                 10 
                 AI436027 
                 OSMR 
                 oncostatin M receptor 
                 PD 
                 1.1E−07 
                 3.7 
               
               
                 11 
                 AI971137 
                 GCLC 
                 glutamate-cysteine ligase, catalytic subunit 
                 PD 
                 1.2E−07 
                 3.9 
               
               
                 12 
                 BQ02487           
                 COL4A3           
                 coltagen, type IV, alpha 3 (Goodpasture antigen) binding protein 
                 PD 
                 2.0E−07 
                 3.6 
               
               
                 13 
                 U52522 
                 ARFIP2 
                 ADP-ribosylation factor interacting protein 2 (arfaptin 2) 
                 PD 
                 2.6E−07 
                 2.8 
               
               
                 14 
                 BM99605           
                 C10orf9 
                 chromosome 10 open reading frame 9 
                 PD 
                 4.2E−07 
                 2.6 
               
               
                 15 
                 AK025452 
                 NIP30 
                 NEFA-interacting nuclear protein NIP30 
                 PD 
                 5.1E−07 
                 3.7 
               
               
                 16 
                 N52048 
                 KIAA077           
                 KIAA0776 protein 
                 PD 
                 5.4E−07 
                 7.2 
               
               
                 17 
                 AA507009 
                 SLC35F2 
                 solute carrier family 35, member F2 
                 PD 
                 6.0E−07 
                 5.8 
               
               
                 18 
                 AA226243 
                 GAMLG 
                 calcium modulating ligand 
                 PD 
                 6.8E−07 
                 5.0 
               
               
                 19 
                 AF005888 
                 NOC4 
                 neighbor of COX4 
                 PD 
                 1.1E−06 
                 4.0 
               
               
                 20 
                 AF012281 
                 PDZK1 
                 PDZ domain containing 1 
                 PD 
                 1.3E−06 
                 4.5 
               
               
                 21 
                 AI188190 
                 DIS3 
                 mitotic control protein dis3 homolog 
                 PD 
                 1.7E−06 
                 3.8 
               
               
                 22 
                 BC001535 
                 CGI-48 
                 CGI-48 protein 
                 PD 
                 2.0E−06 
                 3.5 
               
               
                 23 
                 NM_0070           
                 CPSF6 
                 cleavage and polyadenylation specific factor 6, 68 kDa 
                 PD 
                 2.2E−06 
                 3.4 
               
               
                 24 
                 NM_0022           
                 KIF3C 
                 kinesin family member 3C 
                 PD 
                 2.2E−06 
                 3.5 
               
               
                 25 
                 BQ135232 
                 CD9 
                 CD9 antigen (p24) 
                 PD 
                 2.2E−06 
                 1.7 
               
               
                 26 
                 BC051322 
                 LRRC8 
                 leucine rich repeat containing 8 
                 PD 
                 2.5E−06 
                 3.4 
               
               
                 27 
                 BC03850           
                 SNF1LK 
                 SNF1-like kinase 
                 PD 
                 2.6E−06 
                 2.8 
               
               
                 28 
                 U78556 
                 CRA 
                 cisplatin resistance associated 
                 PD 
                 2.7E−06 
                 3.7 
               
               
                 29 
                 BC035625 
                 EGR2 
                 early growth response 2 (Krox-20 homolog, Drosophila) 
                 PD 
                 3.4E−06 
                 3.0 
               
               
                 30 
                 X52426 
                 KRT13 
                 keratin 13 
                 PD 
                 1.9E−05 
                 3.4 
               
               
                 31 
                 NM_0055           
                 BCAT1 
                 branched chain aminotransferase 1, cytosolic 
                 PD 
                 2.3E−05 
                 1.7 
               
               
                 32 
                 NM_0066           
                 SDCCAG 
                 serologically defined colon cancer antigen 3 
                 PR 
                 2.6E−05 
                 3.7 
               
               
                 33 
                 AA46409           
                 PIGK 
                 phosphatidylinositol glycan, class K 
                 PD 
                 3.2E−05 
                 1.1 
               
               
                 34 
                 AA96118           
                 MRPS9 
                 mitochondrial ribosomal protein S9 
                 PD 
                 9.8E−05 
                 2.3 
               
               
                 35 
                 NM_0181           
                 ASPM 
                 asp (abnormal spindle)-like, microcephaly associated ( Drosophila ) 
                 PR 
                 2.3E−04 
                 2.8 
               
               
                 36 
                 NM_0227           
                 ACBD3 
                 acyl-Coenzyme A binding domain containing 3 
                 PD 
                 2.4E−04 
                 3.8 
               
               
                 37 
                 AA160544 
                 ZNF325 
                 zinc finger protein 325 
                 PR 
                 2.7E−04 
                 4.5 
               
               
                 38 
                 AK05765           
                 LOC2855 
                 hypothetical protein LOC285513 
                 PD 
                 2.7E−04 
                 3.8 
               
               
                 39 
                 NM_0033           
                 TSSC1 
                 tumor suppressing subtransferable candidate 1 
                 PD 
                 2.9E−04 
                 4.7 
               
               
                 40 
                 BC007451 
                 XAB1 
                 XPA binding protein 1 
                 PD 
                 3.0E−04 
                 1.3 
               
               
                 41 
                 BC03546           
                 HNLF 
                 putative NFkB activating protein HNLF 
                 PR 
                 3.5E−04 
                 1.1 
               
               
                 42 
                 CK00409           
                 EIF4EBP 
                 eukaryotic translation initiation factor 4E binding protein 2 
                 PR 
                 3.6E−04 
                 1.4 
               
               
                 43 
                 NM_1446           
                 MGC232           
                 hypothetical protein MGC23280 
                 PR 
                 4.2E−04 
                 2.3 
               
               
                 44 
                 NM_0046           
                 SSA2 
                 Sjogren syndrome antigen A2 (60 kDa, ribonucleoprotein autoantige 
                 PR 
                 4.2E−04 
                 1.2 
               
               
                 45 
                 NM_0027           
                 PRKACA 
                 protein kinase, cAMP-dependent, catalytic, alpha 
                 PR 
                 5.0E−04 
                 1.2 
               
               
                 46 
                 NM_0051           
                 FEZ2 
                 fasciculation and elongation          rotein zeta 2 (zygin II) 
                 PD 
                 6.1E−04 
                 3.3 
               
               
                 47 
                 NM_0058           
                 SRRM1 
                 serine/arginine repetitive matrix 1 
                 PR 
                 7.0E−04 
                 1.4 
               
               
                 48 
                 NM_0062           
                 PDGFRL 
                 platelet-derived growth factor receptor-like 
                 PD 
                 7.0E−04 
                 2.4 
               
               
                 49 
                 AI096936 
                 SNX13 
                 sorting nexin 13 
                 PR 
                 8.4E−04 
                 1.6 
               
               
                 50 
                 NM_0147           
                 KIAA025           
                 KIAA0258 gene product 
                 PD 
                 8.9E−04 
                 2.5 
               
               
                 51 
                 BF973104 
                 TOM7 
                 homolog of Tom7 ( S. cerevisiae ) 
                 PR 
                 1.0E−03 
                 1.5 
               
               
                   
               
               
                   (*) The 12 and 51 gene sets were listed as the rank-order of permutational p-values that were less than 0.001.    
               
             
          
         
       
     
         [0170]    
       
         
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
             
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 4 
               
               
                   
               
               
                   
               
             
             
               
                 A List of 132 Candidate Genes for Discriminating Responder 
                   
               
               
                 (PR) from Non-responder (PD) to Gefitinib 
               
             
          
           
               
                 Rank 
                 GenBank 
                 Gene 
                   
                   
                   
               
               
                 Order 
                 ID 
                 Symbol 
                 Gene Name 
                 Nucleotides 
               
               
                   
               
             
          
           
               
                 1 
                 NM_024829 
                 FLJ22662 
                 hypothetical protein 
                 ATACGGCATCCATGAAATATAT 
                   
               
               
                   
               
               
                   
                   
                   
                 FLJ22662 
                 CATGCGATACAACAATTATAAG 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGGATCCTTACAGTAGAGGTG 
               
               
                   
               
               
                   
                   
                   
                   
                 ACCCCTGTAATACCATCTGCTG 
               
               
                   
               
               
                   
                   
                   
                   
                 CCGTGAGGACCTGAACTCACCT 
               
               
                   
               
               
                   
                   
                   
                   
                 AACCCAAGTCCTGGAGGTTGTT 
               
               
                   
               
               
                   
                   
                   
                   
                 ATGACACAAAGGTGGCAGATAT 
               
               
                   
               
               
                   
                   
                   
                   
                 CTACCTAGCATCTCAGTACACA 
               
               
                   
               
               
                   
                   
                   
                   
                 TCCTATGCCATAAGTGGTCCCA 
               
               
                   
               
               
                   
                   
                   
                   
                 CAGTACAAGGTGGCCTCCCTGT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTTCGCTGGGACCGTTTCAAC 
               
               
                   
               
               
                   
                   
                   
                   
                 AAAACTCTACATCAGGGCATGC 
               
               
                   
               
               
                   
                   
                   
                   
                 CAGAGGTCTACAACTTTGATTT 
               
               
                   
               
               
                   
                   
                   
                   
                 TATTACCATGAAACCAATTTTG 
               
               
                   
               
               
                   
                   
                   
                   
                 AAACTTGATATAAAATGAAGGA 
               
               
                   
               
               
                   
                   
                   
                   
                 GGGAGATGACGGACTAGAAGAC 
               
               
                   
               
               
                 2 
                 BC009799 
                 AREG 
                 amphiregulin 
                 CTCCACTCGCTCTTCCAACACC 
               
               
                   
               
               
                   
                   
                   
                 (schwannoma-derived 
                 CGCTCGTTTTGCGGCAGCTCGT 
               
               
                   
               
               
                   
                   
                   
                 growth factor) 
                 GTCCCAGAGACCGAGTTGCCCC 
               
               
                   
               
               
                   
                   
                   
                   
                 AGAGACCGAGACGCCGCCGCTG 
               
               
                   
               
               
                   
                   
                   
                   
                 CGAAGGACCAATGAGAGCCCCG 
               
               
                   
               
               
                   
                   
                   
                   
                 CTGCTACCGCCGGCGCCGGTGG 
               
               
                   
               
               
                   
                   
                   
                   
                 TGCTGTCGCTCTTGATACTCGG 
               
               
                   
               
               
                   
                   
                   
                   
                 CTCAGGCCATTATGCTGCTGGA 
               
               
                   
               
               
                   
                   
                   
                   
                 TTGGACCTCAATGACACCTACT 
               
               
                   
               
               
                   
                   
                   
                   
                 CTGGGAAGCGTGAACCATTTTC 
               
               
                   
               
               
                   
                   
                   
                   
                 TGGGGACCACAGTGCTGATGGA 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTGAGGTTACCTCAAGAAGTG 
               
               
                   
               
               
                   
                   
                   
                   
                 AGATGTCTTCAGGGAGTGAGAT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTCCCCTGTGAGTGAAATGCCT 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTAGTAGTGAACCGTCCTCGG 
               
               
                   
               
               
                   
                   
                   
                   
                 GAGCCGACTATGACTACTCAGA 
               
               
                   
               
               
                   
                   
                   
                   
                 AGAGTATGATAACGAACCACAA 
               
               
                   
               
               
                   
                   
                   
                   
                 ATACCTGGCTATATTGTCGATG 
               
               
                   
               
               
                   
                   
                   
                   
                 ATTCAGTCAGAGTTGAACAGGT 
               
               
                   
               
               
                   
                   
                   
                   
                 AGTTAAGCCCCCCCAAAACAAG 
               
               
                   
               
               
                   
                   
                   
                   
                 ACGGAAAGTGAAAATACTTCAG 
               
               
                   
               
               
                   
                   
                   
                   
                 ATAAACCCAAAAGAAAGAAAAA 
               
               
                   
               
               
                   
                   
                   
                   
                 GGGAGGCAAAAATGGAAAAAAT 
               
               
                   
               
               
                   
                   
                   
                   
                 AGAAGAAACAGAAAGAAGAAAA 
               
               
                   
               
               
                   
                   
                   
                   
                 ATCCATGTAATGCAGAATTTCA 
               
               
                   
               
               
                   
                   
                   
                   
                 AAATTTCTGCATTCACGGAGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 TGCAAATATATAGAGCACCTGG 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGCAGTAACATGCAAATGTCA 
               
               
                   
               
               
                   
                   
                   
                   
                 GCAAGAATATTTCGGTGAACGG 
               
               
                   
               
               
                   
                   
                   
                   
                 TGTGGGGAAAAGTCCATGAAAA 
               
               
                   
               
               
                   
                   
                   
                   
                 CTCACAGCATGATTGACAGTAG 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTATCAAAAATTGCATTAGCA 
               
               
                   
               
               
                   
                   
                   
                   
                 GCCATAGCTGCCTTTATGTCTG 
               
               
                   
               
               
                   
                   
                   
                   
                 CTGTGATCCTCACAGCTGTTGC 
               
               
                   
               
               
                 3 
                 Nm_014325 
                 C0R01C 
                 coronin, actin binding 
                 GATAGGCCACATTCCAGTAAGA 
               
               
                   
               
               
                   
                   
                   
                 protein, 1C 
                 ACTCAATTTGTCTCCCAAATTT 
               
               
                   
               
               
                   
                   
                   
                   
                 GCAGAAACAAAACGTGATTTAA 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGCTGAGCTTTTTATCAGAAA 
               
               
                   
               
               
                   
                   
                   
                   
                 GCTTTTTTGATGTTTTAAGTGT 
               
               
                   
               
               
                   
                   
                   
                   
                 TATGTGACTTGTTGAACTTTTT 
               
               
                   
               
               
                   
                   
                   
                   
                 AAAAAGTGCTACTTTTAAAATC 
               
               
                   
               
               
                   
                   
                   
                   
                 CCAGATACTCTGAATTTTAGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 AACAAACTAATTCTGATTGTGT 
               
               
                   
               
               
                   
                   
                   
                   
                 CGTGCCCAAGTACCCTTTTTTT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTAATGAGTAGGGACCAATGC 
               
               
                   
               
               
                   
                   
                   
                   
                 CACATTGCTTTTTATATTTCTT 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTTTTTTAATGTTGCCAAAAC 
               
               
                   
               
               
                   
                   
                   
                   
                 CAAAAGTAGCTTTGTTTTCCTT 
               
               
                   
               
               
                   
                   
                   
                   
                 TGTATTTTGCTACTTTGCAGTA 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTGTGTGTGTGGTTTTTTTTC 
               
               
                   
               
               
                   
                   
                   
                   
                 CTTAATTTGAAAGGGACAGCAC 
               
               
                   
               
               
                   
                   
                   
                   
                 TGTGTATGTTTA 
               
               
                   
               
               
                 4 
                 BC010488 
                 AVEN 
                 apoptosis, caspase acti- 
                 AGGAGACCATTTGGAAGAAGAA 
               
               
                   
               
               
                   
                   
                   
                 vation inhibitor 
                 CTAGATCTGTTGCTTAATTTAG 
               
               
                   
               
               
                   
                   
                   
                   
                 ATGCACCTATAAAAGAGGGAGA 
               
               
                   
               
               
                   
                   
                   
                   
                 TAACATCTTACCAGATCAGACG 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTCAGGACCTGAAATCCAAGG 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGATGGGGAGGTGGTCCAAGA 
               
               
                   
               
               
                   
                   
                   
                   
                 GGAAGAAGTTTGTGCAAAACCA 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTGTGACTGAAGAAAAAAACA 
               
               
                   
               
               
                   
                   
                   
                   
                 TGGAACCTGAGCAACCAAGTAC 
               
               
                   
               
               
                   
                   
                   
                   
                 CTCCAAAAATGTTACCGAGGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 GAGCTGGAAGACTGGTTGGACA 
               
               
                   
               
               
                   
                   
                   
                   
                 GCATGATTTCCTAAAAAGGGGA 
               
               
                   
               
               
                   
                   
                   
                   
                 AAAAAAGTGCCTGAAGCAAATC 
               
               
                   
               
               
                   
                   
                   
                   
                 TTGGTTGCCTTCTAACGGCAGG 
               
               
                   
               
               
                   
                   
                   
                   
                 TGGGCATAAGGCTGTCCTTCAG 
               
               
                   
               
               
                   
                   
                   
                   
                 GACCAGCCAGTTTACAAGCATG 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTCAAGCTAGTGTGTTCCATT 
               
               
                   
               
               
                   
                   
                   
                   
                 ATGCTCACAGCAGTAAATGCCT 
               
               
                   
               
               
                   
                   
                   
                   
                 ACCTCTGTGTTTGACATCTGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 AGAATACATTGAAGCAGCTTGT 
               
               
                   
               
               
                   
                   
                   
                   
                 TGCATTTGTTTTTCTGGCTTAG 
               
               
                   
               
               
                   
                   
                   
                   
                 TAATCTAATAGATTTCCTTAAG 
               
               
                   
               
               
                   
                   
                   
                   
                 GGCAGGAGATAGACTCTGGCCC 
               
               
                   
               
               
                   
                   
                   
                   
                 TTGTTTCTAGCCTCCTTCCTTG 
               
               
                   
               
               
                   
                   
                   
                   
                 CAGTGTTTACAACATAGCCAGT 
               
               
                   
               
               
                   
                   
                   
                   
                 GTTTACAGCATAGCA 
               
               
                   
               
               
                   
               
             
          
           
               
                 13: U52522   ARFIP2 
                   
               
             
          
           
               
                 1 
                 tggagcccga ggtccccgcg cggcccgggc ctggcgccct gaggggaaga gcggcccggc 
                   
               
               
                   
               
               
                 61 
                 ccgagccatg acggacggga tcctagggaa ggcagccaca atggagatcc ctatccacgg 
               
               
                   
               
               
                 121 
                 gaacggcgaa gccaggcagc ttcctgaaga tgatgggctg gagcaggacc tccagcaggt 
               
               
                   
               
               
                 181 
                 gatggtgtca ggacccaacc tcaatgaaac cagcattgtg tctggtggct atgggggctc 
               
               
                   
               
               
                 241 
                 tggtgatgga ctcatcccca cagggtctgg ccgccatcca tctcacagca ccactccttc 
               
               
                   
               
               
                 301 
                 tggccctgga gatgaggtgg ctcggggcat tgctggagaa aagtttgaca tcgtcaagaa 
               
               
                   
               
               
                 361 
                 atggggcatc aacacctata agtgcacaaa gcaactgtta tcagaacgat ttggtcgagg 
               
               
                   
               
               
                 421 
                 ctcacggact gtggacctgg agctagagct gcagattgag ttgctgcgtg agacgaagcg 
               
               
                   
               
               
                 481 
                 caagtatgag agtgtcctgc agctgggccg ggcactgaca gcccacctct acagcctgct 
               
               
                   
               
               
                 541 
                 gcagacccag catgcactgg gtgatgcctt tgctgacctc agccagaagt ccccagagct 
               
               
                   
               
               
                 601 
                 tcaggaggaa tttggctaca atgcagagac acagaaacta ctatgcaaga atggggaaac 
               
               
                   
               
               
                 661 
                 gctgctagga gccgtgaact tctttgtctc tagcatcaac acattggtca ccaagaccat 
               
               
                   
               
               
                 721 
                 ggaagacacg ctcatgactg tgaaacagta tgaggctgcc aggctggaat atgatgccta 
               
               
                   
               
               
                 781 
                 ccgaacagac ttagaggagc tgagtctagg cccccgggat gcagggacac gtggtcgact 
               
               
                   
               
               
                 841 
                 tgagagtgcc caggccactt tccaggccca tcgggacaag tatgagaagc tgcggggaga 
               
               
                   
               
               
                 901 
                 tgtggccatc aagctcaagt tcctggaaga aaacaagatc aaggtgatgc acaagcagct 
               
               
                   
               
               
                 961 
                 gctgctcttc cacaatgctg tgtccgccta ctttgctggg aaccagaaac agctggagca 
               
               
                   
               
               
                 1021 
                 gaccctgcag cagttcaaca tcaagctgcg gcctccagga gctgagaaac cctcctggct 
               
               
                   
               
               
                 1081 
                 agaggagcag tgagctgctc ccagcccaac ttggctatca agaaagacat tgggaagggc 
               
               
                   
               
               
                 1141 
                 agccccaggg tgtgggagat tggacatggt acatcctttg tcacttgccc tctggcttgg 
               
               
                   
               
               
                 1201 
                 gctccttttt ctggctgggg cctgacacca gttttgccca cattgctatg gtgggaagag 
               
               
                   
               
               
                 1261 
                 tgcctggagg cccagaagtt gctgccctgt ctatcttcct ggccacaggg cttcattccc 
               
               
                   
               
               
                 1321 
                 agatcttttc cttccacttc acagccaacg gctatgacaa aaccactccc tggccaatgg 
               
               
                   
               
               
                 1381 
                 catcactctt caggctgggg tgtgctccct gaccaatgac agagcctgaa aatgccctgt 
               
               
                   
               
               
                 1441 
                 cagccaatgg cagctcttct cggactcccc tgggccaatg atgttgcgtc taataccctt 
               
               
                   
               
               
                 1501 
                 tgtctctcct ctatgcgtgc ccattgcaga gaaggggact gggaccaaag gggtggggat 
               
               
                   
               
               
                 1561 
                 aatggggagc cccattgctg gccttgcatc tgaataggcc taccctcacc cacccaccca 
               
               
                   
               
               
                 1621 
                 gtttaattgt gcttagagcc caagaagatt ggga 
               
               
                   
               
             
          
           
               
                 14: BM996053   C10orf9 
                   
               
             
          
           
               
                 1 
                 tttttttttt ttttttttaa agtagtttaa taaactccac aaaataatag cagatgcatt 
                   
               
               
                   
               
               
                 61 
                 gaaatattta cataattcga ttttcaaatc tctcattcaa ataaaaggga taaaaataaa 
               
               
                   
               
               
                 121 
                 atttctgcct ttacggcagc agaacctctt tcctgaaatg gattggtaaa ataagatact 
               
               
                   
               
               
                 181 
                 tcactggnag aggaactaat ttatgtttaa gaggtattca tattcagcta agaaaataca 
               
               
                   
               
               
                 241 
                 accctttttc agctatatag attagggaat ataaaatgat attttctaca ttttttgacc 
               
               
                   
               
               
                 301 
                 tgtattcaaa gttctaaatt caactttgac ttgaagagag aaggtgattt tggtacccat 
               
               
                   
               
               
                 361 
                 acagagtaga tcatcacaat tacaatggaa agataattaa cgttttatat gctgtttatt 
               
               
                   
               
               
                 421 
                 tgcttttgaa agtttgggtc agaaaggctg tgataataat tctggcccaa acaggtatgc 
               
               
                   
               
               
                 481 
                 ttatacctga cacaaatttc actaaaacct aacacttttg gcttggagtt cttgggattt 
               
               
                   
               
               
                 541 
                 cgactttctg agtcccttcc atttccaaag catgtttcat tgagagcagg caatgtttgg 
               
               
                   
               
               
                 601 
                 ggatcaggtg tatgattcaa gactaattaa gatgccaaag ttttccaagc tc 
               
               
                   
               
             
          
           
               
                 15: AK025452   NIP30 
                   
               
             
          
           
               
                 1 
                 attgtggcgg tgaggaacag gaagccctga agggtcaaaa gaaatacaaa agcaaaggct 
                   
               
               
                   
               
               
                 61 
                 attttctttt tttttttctt tctttcattc cttccttcct ctgtttcttt ctttcttcct 
               
               
                   
               
               
                 121 
                 ttcatttttt tttctttttt aagagcgagc ggctctgcgg tggcggtttg gggtgggcgc 
               
               
                   
               
               
                 181 
                 cgccgaggtg aggtcgtctc gcctcccgcg cgccggtaga ttggttgttt cattatggat 
               
               
                   
               
               
                 241 
                 ggaggggatg atggtaacct tattatcaaa aagaggtttg tgtctgaggc agaactagat 
               
               
                   
               
               
                 301 
                 gaacggcgca aaaggaggca agaagaatgg gagaaagttc gaaaacctga agatccagaa 
               
               
                   
               
               
                 361 
                 gaatgtccag aggaggttta tgaccctcga tctctatatg aaaggctaca ggaacagaag 
               
               
                   
               
               
                 421 
                 gacaggaagc agcaggagta cgaggaacag ttcaaattca aaaacatggt aagaggctta 
               
               
                   
               
               
                 481 
                 gatgaagatg agaccaactt ccttgatgag gtttctcgac agcaggaact aatagaaaag 
               
               
                   
               
               
                 541 
                 caacgaagag aagaagaact gaaagaactg aaggaataca gaaataacct caagaaggtt 
               
               
                   
               
               
                 601 
                 ggaatttctc aagagaacaa gaaggaagtg gaaaagaaac tgactgtgaa gcctatagaa 
               
               
                   
               
               
                 661 
                 accaagaaca agttctccca ggcgaagctg ttggcaggag ctgtgaagca taagagctca 
               
               
                   
               
               
                 721 
                 gagagtggca acagtgtgaa aagactgaaa ccggaccctg agccagatga caagaatcaa 
               
               
                   
               
               
                 781 
                 gagccctcat cctgcaagtc tctcggaaac acctccctga gtggcccctc catccactgc 
               
               
                   
               
               
                 841 
                 ccctctgctg cagtatgtat cggcatcctc ccaggcctgg gtgcctactc tgggagcagc 
               
               
                   
               
               
                 901 
                 gactccgagt ccagctcaga cagcgaaggc accatcaatg ccaccggaaa gattgtctcc 
               
               
                   
               
               
                 961 
                 tccatcttcc gaaccaacac cttcctcgag gccccctagt ttctccgtcc ctacacaggg 
               
               
                   
               
               
                 1021 
                 agctcctccc caagggtaga tcggaccgtt catgctgcct ataggcatta tgtccctcaa 
               
               
                   
               
               
                 1081 
                 aaaaaaactc ctttgcctgc atcctgtgta caacatgaca tttttaacca atccaatcta 
               
               
                   
               
               
                 1141 
                 aaaatgtgcc agaatccacc tgtggcccga atcgtgtttg gttcctcttt ctactccact 
               
               
                   
               
               
                 1201 
                 gcagatgacc aaacctgtcc cgctgccact ttcctcactg atattgggag gagggcaagg 
               
               
                   
               
               
                 1261 
                 cccagccgaa gttccactaa aaatgcccca ggagaatagg caccggctgg cttgccaaag 
               
               
                   
               
               
                 1321 
                 ggtttgggtt ttattgcttt ctgttttttc ttttcccgac agcacaaaga agtaagggca 
               
               
                   
               
               
                 1381 
                 gttattggac aggtgttatt taaacattct attgtaaatg aatgtgttgt ttggttctac 
               
               
                   
               
               
                 1441 
                 tgcattgtgg agcatgcggg ggaagagaac tgacccaggt aatgaaatgg agcccttccc 
               
               
                   
               
               
                 1501 
                 tggaactaac cagtccttga tgttgtgtga ctaagtaaag atgataaacc ccatctgctg 
               
               
                   
               
               
                 1561 
                 ggggtgtcac ttcacactcg gcatgcattg tgaaagcttt ccataccctt ggccattccc 
               
               
                   
               
               
                 1621 
                 tctctcctct ctctccaacc ccatttatgc aggaggggac tgctaacaag aacgcttcca 
               
               
                   
               
               
                 1681 
                 tctcaaacct tttctctgcc tgggaaatta ttttatgttt gtttttgaaa taaaggattt 
               
               
                   
               
               
                 1741 
                 agtttaagat tctaaatttt agagaaacaa acgtaggcct tgtttactaa tagccagaca 
               
               
                   
               
               
                 1801 
                 tcagaactgc aggtaggtat gttaatgaga tgacttattt ctggcagctc ctggaatcct 
               
               
                   
               
               
                 1861 
                 aatattgtaa atgagtggga cacacttgca tattgtgacc attctattga ggcccttctc 
               
               
                   
               
               
                 1921 
                 tgtttaatgc atattatact tgtgctttta actgtggaat ctatttctaa cctaaaaaaa 
               
               
                   
               
               
                 1981 
                 aaaaaaaaaa 
               
               
                   
               
             
          
           
               
                 16: N52048   KIAA0776 
                   
               
             
          
           
               
                 1 
                 aaatttctgt attttagttt tgaagtgctt tctatttaaa aataaaaaac atgatttatt 
                   
               
               
                   
               
               
                 61 
                 ttcatttctg acacagaagt gtttctttta aaaaaaaaag accacatttt aaatttctgc 
               
               
                   
               
               
                 121 
                 ttaaatgtat ataaagtata catttaagta tactggcact cgcaacaaat gaatccttcc 
               
               
                   
               
               
                 181 
                 ccagggataa atggattgga aaatttgttt ttcattcaac atttggaaag agaacaaacc 
               
               
                   
               
               
                 241 
                 tgaaatatgt aatttttaaa attatgtgaa aataatgtga aaaatttcat atagtatttg 
               
               
                   
               
               
                 301 
                 tgtgaaaatc aggtggaaaa aaacttccat gaagaaccca atttaccaaa attcnccatc 
               
               
                   
               
               
                 361 
                 nttttaagat ttacnttttn aataccatac tactggtttt aacnggaatt ggggtgtggt 
               
               
                   
               
               
                 421 
                 atgagggggg ttttgcnggg gagangggga 
               
               
                   
               
             
          
           
               
                 17: AA507009:   SLC35F2 
                   
               
             
          
           
               
                 1 
                 tatttccatg aattcaaagc cttttaatga tgtgaacact tactccccat ttctttttta 
                   
               
               
                   
               
               
                 61 
                 cattgttaca aaaaatttac atacagtttt ctgaaagtgg cattttgttg gttgttatta 
               
               
                   
               
               
                 121 
                 tactgatgac acatattaac actttgtatt gaagaagtat cataaaaatc acagggcatt 
               
               
                   
               
               
                 181 
                 acagattttt gataagaagt agtaatagca ttgtctttta acagctggag gctcccaggc 
               
               
                   
               
               
                 241 
                 atactctttg gtgagaaatg attaatttta tattttcatt ttgatgagaa tcttttcttg 
               
               
                   
               
               
                 301 
                 tttttaccag ttataaaaac aaagcttttt ctttgttgtg atactgtgca ctaagactta 
               
               
                   
               
               
                 361 
                 gtttcttgag ctgatgctaa ataaaatgag atcaatagga atattccggg aggtcgtgag 
               
               
                   
               
               
                 421 
                 aagtttttag aaaggatggc atctacatat atatggagct ctgaaaactg ttggagagta 
               
               
                   
               
               
                 481 
                 tgacctggga ctgaaactgt ggagcaca 
               
               
                   
               
             
          
           
               
                 18: AA226243:   GAMLG 
                   
               
             
          
           
               
                 1 
                 tcgacatcta atcctcattc ttatgaactt ggtattatgt gaatccattg ggaaatgaag 
                   
               
               
                   
               
               
                 61 
                 actcagagag gttaaaccat ttgcccaagg tcacacagct actaagcggt gctaggatta 
               
               
                   
               
               
                 121 
                 aaccctggca gtttcagtcc ttaaccaaag ggttaagcgc tttacatata tatacctgta 
               
               
                   
               
               
                 181 
                 tattatcaat tttcaaataa tttgaaatag taaaatgcag ttcctctggt cctgaatatg 
               
               
                   
               
               
                 241 
                 aggtaatctt cctatggttc agaagacatt tcagcatttc ctaatatatc ataatgagtc 
               
               
                   
               
               
                 301 
                 cattttggtt gtaccatgat gtagtcattt tgttttatag tatattaaag aatgcagaaa 
               
               
                   
               
               
                 361 
                 agcatagctc atagttccag tgtcttgctc tgaggttttt ccattcagta gacattttaa 
               
               
                   
               
               
                 421 
                 gtatatgtac caggcacata aatatccaga taattaaagt ttatatcatt aagcn 
               
               
                   
               
             
          
           
               
                 19: AF005888   NOC4 
                   
               
             
          
           
               
                 1 
                 gcgccatcaa tcgccgccgc ctcgtcccgc ttctcggctg aggcgccgcg cggccaggca 
                   
               
               
                   
               
               
                 61 
                 gcgggtccag gcctcagccg cgcgcccagg ggcctccggg gccctcccgg gtcagcatgc 
               
               
                   
               
               
                 121 
                 ccggggtgaa actgaccacc caggcctact gcaagatggt gctgcacggc gccaagtacc 
               
               
                   
               
               
                 181 
                 cgcactgcgc cgtcaacggg ctcctggtgg ccgagaagca gaagccgcgt aaggagcacc 
               
               
                   
               
               
                 241 
                 tccccctggg cggccccggc gcccaccaca ccctcttcgt ggactgcatc cccctcttcc 
               
               
                   
               
               
                 301 
                 acggcaccct ggccctcgcc cccatgctgg aggtggctct caccctgatt gattcatggt 
               
               
                   
               
               
                 361 
                 gcaaagatca tagctacgtg attgctggtt attatcaagc taatgagcga gtaaaggatg 
               
               
                   
               
               
                 421 
                 ccagtccaaa ccaggttgca gagaaggtgg cctccagaat cgccgagggc ttcagcgaca 
               
               
                   
               
               
                 481 
                 ctgcgctcat catggtagac aacaccaagt ttacgatgga ctgcgtagcg cctacgatcc 
               
               
                   
               
               
                 541 
                 acgtgtacga gcaccatgag aacagatggc ggtgcagaga cccacaccat gactactgtg 
               
               
                   
               
               
                 601 
                 aagactggcc agaggcacag aggatctcag cctcgctcct ggacagccgg tcctacgaga 
               
               
                   
               
               
                 661 
                 cgctcgtgga tttcgataac cacctggatg acattcggaa tgactggaca aacccagaga 
               
               
                   
               
               
                 721 
                 tcaataaagc tgtcctacac ttgtgctagg caggcaccgc tgtgactggg ctccgggcct 
               
               
                   
               
               
                 781 
                 ttcccactac gttgaagaag aaaacctatt tttaaatgta aataaaatat ctggtagcct 
               
               
                   
               
               
                 841 
                 gtgtggaaag ctgaccgttt taagaagtgg catgtgcctt gaaagggggc agaatgttca 
               
               
                   
               
               
                 901 
                 gtcggtcgtg tttttaacac agagtctcta gaagaggtgc agacatcccg tctgactgtc 
               
               
                   
               
               
                 961 
                 cctgtggact ctctcagttg tatgttgcta taatcctcca aatcaaagct ctttctgctt 
               
               
                   
               
               
                 1021 
                 gtgcaagatt gttcctatta aacagtttta actaaccttt a 
               
               
                   
               
             
          
           
               
                 20: AF012281   PDZK1 
                   
               
             
          
           
               
                 1 
                 gaattccggg cagctcctct tccatctcca gaaatgacct ccaccttcaa cccccgagaa 
                   
               
               
                   
               
               
                 61 
                 tgtaaactgt ccaagcaaga agggcaaaac tatggcttct tcctgcgaat tgagaaggac 
               
               
                   
               
               
                 121 
                 accgagggcc acctggtccg ggtggttgag aagtgtagcc cagcagagaa ggctggcctt 
               
               
                   
               
               
                 181 
                 caagatggag acagagttct taggatcaat ggtgtctttg tggacaaaga agaacatatg 
               
               
                   
               
               
                 241 
                 caggttgtgg atctggtcag aaagagtggg aattcagtga ctttactagt tctggatggg 
               
               
                   
               
               
                 301 
                 gattcctatg agaaagcagt gaaaacacgg gtggacttga aagagttggg tcaaagtcag 
               
               
                   
               
               
                 361 
                 aaggagcaag gtttgagtga taatatactt tcccctgtga tgaatggagg tgtgcaaact 
               
               
                   
               
               
                 421 
                 tggacccagc cccggctctg ctatctcgtg aaggaaggag gcagctatgg cttctctctg 
               
               
                   
               
               
                 481 
                 aaaactgtcc aaggtaaaaa gggggtgtac atgactgata ttacacctca aggtgtggct 
               
               
                   
               
               
                 541 
                 atgagagctg gagttctggc tgatgatcac ttgattgaag tgaatggaga gaatgtagag 
               
               
                   
               
               
                 601 
                 gatgccagcc atgagaaagt ggttgaaaag gtgaagaagt caggaagccg tgtcatgttc 
               
               
                   
               
               
                 661 
                 ctgctggtgg acaaagaaac tgacaagcgt catgttgagc agaagataca attcaaaaga 
               
               
                   
               
               
                 721 
                 gaaacagcca gtttgaaact gttaccccac cagccccgaa ttgtggagat gaagaaagga 
               
               
                   
               
               
                 781 
                 agcaatggct atggtttcta tctgagggca ggctcagaac agaaaggtca aatcatcaag 
               
               
                   
               
               
                 841 
                 gacatagatt ctggaagtcc agcagaggag gctggcttga agaacaatga tctggtagtt 
               
               
                   
               
               
                 901 
                 gctgtcaacg gcgagtctgt ggaaaccctg gatcatgaca gtgtggtaga aatgattaga 
               
               
                   
               
               
                 961 
                 aagggtggag atcagacttc actgttggtg gtagacaaag agacggacaa catgtacaga 
               
               
                   
               
               
                 1021 
                 ctggctcatt tttctccatt tctctactat caaagtcaag aactgcccaa tggctctgtc 
               
               
                   
               
               
                 1081 
                 aaggaggctc cagctcctac tcccacttct ctggaagtct caagtccacc agatactaca 
               
               
                   
               
               
                 1141 
                 gaggaagtag atcataagcc taaactctgc aggctggcta aaggtgaaaa tggctatggc 
               
               
                   
               
               
                 1201 
                 tttcacttaa atgcgattcg gggtctgcca ggctcattca tcaaagaggt acagaagggc 
               
               
                   
               
               
                 1261 
                 ggtcctgctg acttggctgg gctagaggat gaggatgtca tcattgaagt gaatggggtg 
               
               
                   
               
               
                 1321 
                 aatgtgctag atgaacccta tgagaaggtg gtggatagaa tccagagcag tgggaagaat 
               
               
                   
               
               
                 1381 
                 gtcacacttc tagtctgtgg aaagaaggcc tatgattatt tccaagctaa gaaaatccct 
               
               
                   
               
               
                 1441 
                 attgtttcct ccctggctga tccacttgac acccctccag attctaaaga aggaatagtg 
               
               
                   
               
               
                 1501 
                 gtggagtcaa accatgactc gcacatggca aaagaacggg cccacagtac agcctcacat 
               
               
                   
               
               
                 1561 
                 tcttcttcca attctgaaga tacagagatg tgatgaaaac aagtaatagc tttggctgtt 
               
               
                   
               
               
                 1621 
                 tatttgatag ctgtttctgg gtatttaata ggaatccttt ctcaaggaat gagttgtgac 
               
               
                   
               
               
                 1681 
                 ctgtttactg tctctttaga agaaaaactc cactggaaac cattcaccat gtgtgactgt 
               
               
                   
               
               
                 1741 
                 cttctgttat catttgtctt acaggcggct attgcagacg gctaatttat gcttaactta 
               
               
                   
               
               
                 1801 
                 ggaagagata aggcaagagc tagatttttt tcatgtgatc ttttccaagc ttcaacttaa 
               
               
                   
               
               
                 1861 
                 cttaactaca tttctctgta tgatgatgtc tcttacttct acaggttcct tgagcaccaa 
               
               
                   
               
               
                 1921 
                 agatgattca taactctgta taggtgacag ctgcttataa aagcatctta gcagataagc 
               
               
                   
               
               
                 1981 
                 ctattaaaat tgtgcttttg taacaatgtt gtggttgcta gaataaatac catgaacccg 
               
               
                   
               
             
          
           
               
                 21: AI188190   DIS3 
                   
               
             
          
           
               
                 1 
                 tttaaaagcc actaattatc tgttttttat tttgtaagta acaagatata gacatttgaa 
                   
               
               
                   
               
               
                 61 
                 tgccaatgtc ttattctgga gagacactgg agctgaagtt caacaatgat cacacttatt 
               
               
                   
               
               
                 121 
                 acctggcaat aaaaacacaa ccatctttcc agtcaggtca aaatatccta ctttttgcct 
               
               
                   
               
               
                 181 
                 ttctaccaaa tcccaaacat tcacagtttt tcaaggacca ctaataaaat acaggaagct 
               
               
                   
               
               
                 241 
                 tttaaagaca gtaagagaac acctagtgta agttaggtga attaaagatg gcaaaggaga 
               
               
                   
               
               
                 301 
                 ttacatcctc aacactgaca gcttccaaga cttagaaaag agattgttcc ttgcttctaa 
               
               
                   
               
               
                 361 
                 aattgttcta ttttcctctg taggaaaatg aaagtttttt cttacaaata ttaaataatc 
               
               
                   
               
               
                 421 
                 aaagtactta cgcaaaatta atctgctcct caatgagatg agcactccat ttaaatgatc 
               
               
                   
               
               
                 481 
                 tttacagatc cctgaagttg ctgtcctgtc actgtattta agtgatggat attcaattga 
               
               
                   
               
               
                 541 
                 attattctgc ataaataatt ctggtcaacc cagacgtata gtagtatgat gggtcagata 
               
               
                   
               
               
                 601 
                 cagtcaactg ttcaataaaa atgcagatgt ctg 
               
               
                   
               
             
          
           
               
                 22: BC001535   CGI-48 
                   
               
             
          
           
               
                 1 
                 ctgcgtttct cctcaaacct aacgatgccg ccggagcgga ggagacgaat gaaactggac 
                   
               
               
                   
               
               
                 61 
                 cggagaaccg gagcgaagcc gaagcggaag cccggaatga ggccggactg gaaagccgga 
               
               
                   
               
               
                 121 
                 gcggggccag gcgggcctcc ccaaaagcct gccccttcat cccagcggaa accgccggcc 
               
               
                   
               
               
                 181 
                 cggccgagcg cggcggccgc tgcgattgca gtcgcggcgg cggaggaaga gagacggctc 
               
               
                   
               
               
                 241 
                 cggcagcgga accgcctgag gctggaggag gacaaaccgg ccgtggagcg gtgcttggag 
               
               
                   
               
               
                 301 
                 gagctggtct tcggcgacgt cgagaacgac gaggatgcgt tgctgcggcg tctgcgaggc 
               
               
                   
               
               
                 361 
                 ccgagggttc aagaacatga agactcgggt gactcagaag tggagaatga agcaaaaggt 
               
               
                   
               
               
                 421 
                 aattttccac ctcaaaagaa gccagtttgg gtggatgaag aagatgaaga tgaggaaatg 
               
               
                   
               
               
                 481 
                 gttgacatga tgaacaatcg gtttcggaag gatatgatga aaaatgctag tgaaagtaaa 
               
               
                   
               
               
                 541 
                 ctttcgaaag acaaccttaa aaagagactt aaagaagaat tccaacatgc catgggagga 
               
               
                   
               
               
                 601 
                 gtacctgcct gggcagagac tactaagcgg aaaacatctt cagatgatga aagtgaagag 
               
               
                   
               
               
                 661 
                 gatgaagatg atttgttgca aaggactggg aatttcatat ccacatcaac ttctcttcca 
               
               
                   
               
               
                 721 
                 agaggaatct tgaagatgaa gaactgccag catgcgaatg ctgaacgtcc tactgttgct 
               
               
                   
               
               
                 781 
                 cggatctcat ctgtgcagtt ccatcccggt gcacagattg tgatggttgc tggattagat 
               
               
                   
               
               
                 841 
                 aatgctgtat cactatttca ggttgatggg aaaacaaatc ctaaaattca gagcatctat 
               
               
                   
               
               
                 901 
                 ttggaaaggt ttccaatctt taaggcttgt tttagtgcta atggggaaga agttttagcc 
               
               
                   
               
               
                 961 
                 acgagtaccc acagcaaggt tctttatgtc tatgacatgc tggctggaaa gttaattcct 
               
               
                   
               
               
                 1021 
                 gtgcatcaag tgagaggttt gaaagagaag atagtgagga gctttgaagt ctccccagat 
               
               
                   
               
               
                 1081 
                 gggtccttct tgctcataaa tggcattgct ggatatttgc atttgctagc aatgaagacc 
               
               
                   
               
               
                 1141 
                 aaagaactga ttggaagcat gaaaattaat ggaagggttg cagcatccac attctcttca 
               
               
                   
               
               
                 1201 
                 gatagtaaga aagtatacgc ctcttcgggg gatggagaag tttatgtttg ggatgtgaac 
               
               
                   
               
               
                 1261 
                 tcaaggaagt gccttaacag atttgttgat gaaggcagtt tatatggatt aagcattgcc 
               
               
                   
               
               
                 1321 
                 acatctagga atggacagta tgttgcttgt ggttctaatt gtggagtggt aaatatatac 
               
               
                   
               
               
                 1381 
                 aatcaagatt cttgtctcca agaaacaaac ccaaagccaa taaaagctat aatgaacttg 
               
               
                   
               
               
                 1441 
                 gttacaggtg ttacttctct gaccttcaat cctactacag aaatcttggc aattgcttca 
               
               
                   
               
               
                 1501 
                 gaaaaaaatg aaagaagcag tcagattggt tcatcttcct tcctgtacag tattttcaaa 
               
               
                   
               
               
                 1561 
                 cttcccagtc attaaaaata agaatatttc tcatgttcat accatggatt tttctccgag 
               
               
                   
               
               
                 1621 
                 aagtggatac tttgccttgg ggaatgaaaa gggcaaggcc ctgatgtata ggttgcacca 
               
               
                   
               
               
                 1681 
                 ttactcagac ttctaaagag actatttgaa gtccagttga gtcacaagag aagcctgtct 
               
               
                   
               
               
                 1741 
                 tgatatatca tctcagaaac tttcctgaat atgtgataat atatggaaaa tgatttatag 
               
               
                   
               
               
                 1801 
                 atccagctgt gcttaagagc cagtaatgtc ttaataaaca tgtggcagct tttgtttgaa 
               
               
                   
               
               
                 1861 
                 aaaaaaaaaa aaaaaaaa 
               
               
                   
               
             
          
           
               
                 23: NM_007007   CPSF6 
                   
               
             
          
           
               
                 1 
                 aattccgggc ggcggcggcc gaggctgaag gaagatggcg gacggcgtgg accacataaa 
                   
               
               
                   
               
               
                 61 
                 catttacgcg gatgtcggcg aagagttcaa ccaggaagct gaatatggtg ggcatgatca 
               
               
                   
               
               
                 121 
                 gatagatttg tatgacgatg tcatatctcc atctgcaaat aatggagatg ccccagaaga 
               
               
                   
               
               
                 181 
                 ccgagattac atggatactc tcccaccaac tgttggtgat gatgtgggta aaggagcagc 
               
               
                   
               
               
                 241 
                 accaaatgtt gtctatacat atactggaaa gagaattgca ttatatattg gaaatctaac 
               
               
                   
               
               
                 301 
                 atggtggaca acagatgaag acttaactga agcagttcat tctttgggag taaatgatat 
               
               
                   
               
               
                 361 
                 tttggagata aaattttttg aaaatcgagc aaatggccag tcaaaggggt ttgcccttgt 
               
               
                   
               
               
                 421 
                 tggtgttgga tctgaagcat cttcaaaaaa gttaatggat ctgttaccta aaagagaact 
               
               
                   
               
               
                 481 
                 tcatggtcag aatcctgttg taactccatg caataaacag ttcctgagtc aatttgaaat 
               
               
                   
               
               
                 541 
                 gcagtccagg aaaactacac aatcaggaca aatgtctggg gaaggtaaag ctggtcctcc 
               
               
                   
               
               
                 601 
                 aggaggcagt tcccgtgcag catttccaca aggtggtaga ggacggggcc gttttccagg 
               
               
                   
               
               
                 661 
                 ggctgttcct ggtggggaca gatttcctgg gccagcagga ccaggagggc cacccccacc 
               
               
                   
               
               
                 721 
                 ttttccagct ggacagactc caccacgtcc acccttaggt cctccaggcc cacctggtcc 
               
               
                   
               
               
                 781 
                 accaggtcct ccacctcctg gtcaggttct gcctcctcct ctagctgggc ctcctaatcg 
               
               
                   
               
               
                 841 
                 aggagatcgc cctccaccac cagttctttt tcctggacaa ccttttgggc agcctccatt 
               
               
                   
               
               
                 901 
                 gggtccactt cctcctggcc ctccacctcc agttccaggc tacggccccc ctcctggccc 
               
               
                   
               
               
                 961 
                 accacctcca caacagggac cacctccacc tccaggcccc tttccacctc gtccacccgg 
               
               
                   
               
               
                 1021 
                 tccacttggg ccacccctta cactagctcc tcctccgcat cttcctggac cacctccagg 
               
               
                   
               
               
                 1081 
                 tgccccaccg ccagctccgc atgtgaaccc agctttcttt cctccaccaa ctaacagtgg 
               
               
                   
               
               
                 1141 
                 catgcctaca tcagatagcc gaggtccacc accaacagat ccatatgggc gacctccacc 
               
               
                   
               
               
                 1201 
                 atatgatagg ggtgactatg gcccccctgg aagggaaatg gatactgcaa gaacgccatt 
               
               
                   
               
               
                 1261 
                 gagtgaagct gaatttgaag aaatcatgaa tagaaatagg gcaatctcaa gcagtgctat 
               
               
                   
               
               
                 1321 
                 ttcgagagct gtgtctgatg ccagtgctgg tgattatggg agtgctattg agacactggt 
               
               
                   
               
               
                 1381 
                 aactgcaatt tctttaatta aacaatccaa agtatctgct gatgatcgtt gcaaagttct 
               
               
                   
               
               
                 1441 
                 tattagttct ttgcaagatt gccttcatgg aattgagtcc aagtcttatg gttctggatc 
               
               
                   
               
               
                 1501 
                 aagacgtgaa cgatcaagag agagggacca tagtagatca cgagaaaaga gtcgacgtca 
               
               
                   
               
               
                 1561 
                 taaatcccgt agtagagacc gtcatgacga ttattacaga gagagaagca gagaacgaga 
               
               
                   
               
               
                 1621 
                 gaggcaccgg gatcgtgacc gagaccgtga ccgagagcgt gaccgagagc gcgaatatcg 
               
               
                   
               
               
                 1681 
                 tcatcgttag aagctgaagg aagaggatca ccttccaaga caaaacagtc ttcatgggcc 
               
               
                   
               
               
                 1741 
                 aaaaatgacg cttgtccagc agtttgcttc ttgtgattga actgaacctg taaggattca 
               
               
                   
               
               
                 1801 
                 tggataaaat gaacaggaat agatctgaat aaagcaaatc tgcataaatg gtaaccagta 
               
               
                   
               
               
                 1861 
                 gctctacttt tattttttat gttgcttaac tgttttattt gaaggaaacc tgtgtgattt 
               
               
                   
               
               
                 1921 
                 aaaaagttat agcttttgca actttattac tggttatata catttggcca ttatgatgtg 
               
               
                   
               
               
                 1981 
                 caagcaattg gaaaaaaagt caagtaaatg cttgtttttg tagtagtttg ttcttgttaa 
               
               
                   
               
               
                 2041 
                 aaatgtttat atgataatgt ctgtaaacag catcactttg attacaatag atgtagtgtt 
               
               
                   
               
               
                 2101 
                 gtaataaact gtttaatggg gctgatgtgt aaagctgttc aagttatttg atgtttacac 
               
               
                   
               
               
                 2161 
                 ctcagggaaa gtcttgtgtt cagcaatatc taaagataat gttactatga caacattttt 
               
               
                   
               
               
                 2221 
                 actgtccttt aaagcattgc aatagcgttt ttggatatgc ctcaatctaa tcttgcgttc 
               
               
                   
               
               
                 2281 
                 agtgaattaa acatagtaat taagtgtctt ttgcccttga ttttgatatt agaataggtg 
               
               
                   
               
               
                 2341 
                 attacatgga tatttaatat ttctatattc tgcttttcta gctgttttta cctagttagc 
               
               
                   
               
               
                 2401 
                 ttgtgacttt gctgaatggt atgtaaactt gtaaaaatag agatttgaca gacatagcaa 
               
               
                   
               
               
                 2461 
                 tctagtcaat gtgtaagggg tcaaaaaaaa cagaggtttt aacacataag taaaaacccg 
               
               
                   
               
               
                 2521 
                 tacatatttg atgtgtaatg caggttaatt acaacacaga tgtaccgaaa cacttaattg 
               
               
                   
               
               
                 2581 
                 tgaaccgcta acattgaaga aattttgaca attccgattt gatgctgcaa ttacttgctg 
               
               
                   
               
               
                 2641 
                 tttttattga tcttatggtt tatttcttaa gccatagtca gtgtaaatac agccctgcag 
               
               
                   
               
               
                 2701 
                 caggtaaatg tgagtaaaga gagccttata ttttccaatt ggtataaaat ttttgaagga 
               
               
                   
               
               
                 2761 
                 tgtgatgttc attaacattc ggttgtattc cccagtattt gtaatgggaa attacagata 
               
               
                   
               
               
                 2821 
                 aaccgtgtct gcacagttta aggaatacta tgtatattca tgcaccgtat tgattcatgc 
               
               
                   
               
               
                 2881 
                 tatagttact taatcaaaga tttttttcaa acctgcctta catataggcc cactttaaaa 
               
               
                   
               
               
                 2941 
                 gcacctgact agcatgtgtt cttgattgca aaattggcag aggcagggtg tcaacttgat 
               
               
                   
               
               
                 3001 
                 taggtgtttt tatgggaatg taatttgaaa tcactacttc agaaatttga cttaaaattc 
               
               
                   
               
               
                 3061 
                 ttgagcacgt taatatgttt ttaagatctg attatctttg agagatcttc tgttaataca 
               
               
                   
               
               
                 3121 
                 cattggttgt taaagagtac ccaaattcta ggacaatgct taaagtgtta aaatacccta 
               
               
                   
               
               
                 3181 
                 gatactgtgt tatgtgcaac tgtagaaacc ctccagaaat ttccactgct gttcttcact 
               
               
                   
               
               
                 3241 
                 ttcatcttgt ctgctatcaa accacttctg acaaaattag ctgttttgaa ttacccatat 
               
               
                   
               
               
                 3301 
                 cactgccagt tttattttaa aatattttgt gtttgaagta tctgtgcatg ggatcgttga 
               
               
                   
               
               
                 3361 
                 tgtttatcag aactgttcac tttcagaaat gattttttaa agcattttgt tgaaatgcgg 
               
               
                   
               
               
                 3421 
                 ttgctt 
               
               
                   
               
             
          
           
               
                 24: NM_002254   KIF3C 
                   
               
             
          
           
               
                 1 
                 tcactctcgc tgccgctgct ccgccccatc cccttctgtt tttctctctc attctccagt 
                   
               
               
                   
               
               
                 61 
                 ggcggcggcg gggaaggcgg aggcagaggc agcagcagcc gcgctggctg caatgaatga 
               
               
                   
               
               
                 121 
                 tcccccagct tggggggagg actccaggtg agcctctgcc ctcgggaggc ccgggacccc 
               
               
                   
               
               
                 181 
                 cggccgccca cgaccggcag cccacgctat ggatccctag aggaaggagg agaagacagc 
               
               
                   
               
               
                 241 
                 tcgccgccca cccccatccc attttcctct tcctttatct cattgttgcc gaagctgttt 
               
               
                   
               
               
                 301 
                 acggcagcgc tccctctgct ccagcatggg gcgggctccg ggcacggctg ctcggcaggc 
               
               
                   
               
               
                 361 
                 gctgctcccg cggcgactgg gggattctgc ctaattcacc tcccagccgg tgcagagagg 
               
               
                   
               
               
                 421 
                 accggagagc ggtggaggcc cggactgcag cagcgttggg gccacctccc agcgtcccca 
               
               
                   
               
               
                 481 
                 ccctaggagg ctgcatgcgg attgaagagc tgcgcctggg ggctgggccg gccccgctga 
               
               
                   
               
               
                 541 
                 tcccgaccta gcgagcagga tagcaggacc gcccaggctg cggaggggct cgggggcagg 
               
               
                   
               
               
                 601 
                 aaggtcagag cagcaagatg gccagtaaga ccaaggccag cgaggccctc aaggtggtgg 
               
               
                   
               
               
                 661 
                 cccggtgccg ccccctcagc aggaaggagg aggctgctgg tcacgagcag atcctgacca 
               
               
                   
               
               
                 721 
                 tggacgtgaa actgggccag gtgaccctgc ggaacccccg cgccgccccg ggggagctgc 
               
               
                   
               
               
                 781 
                 ccaagacctt cacctttgac gccgtgtatg atgccagctc caagcaggcc gacctgtatg 
               
               
                   
               
               
                 841 
                 acgaaaccgt gaggcccctg atagactccg tgctccaggg tttcaatggc acggtgtttg 
               
               
                   
               
               
                 901 
                 cctatggcca gacgggcact ggcaagacct ataccatgca ggggacctgg gtggagcccg 
               
               
                   
               
               
                 961 
                 agctgcgcgg ggtcatcccg aatgcctttg agcacatctt cacccacatc tcccgctccc 
               
               
                   
               
               
                 1021 
                 agaaccaaca gtacctggtc cgggcctcct atttggagat ctaccaggaa gagattcgag 
               
               
                   
               
               
                 1081 
                 acctgctctc caaggagccg ggcaagaggc tagagctgaa agagaacccc gagactggcg 
               
               
                   
               
               
                 1141 
                 tctacatcaa ggacctctcc tccttcgtca ccaagaatgt caaggagatt gagcatgtga 
               
               
                   
               
               
                 1201 
                 tgaacctggg gaaccagacc cgggctgtgg gcagcaccca catgaatgag gtcagctccc 
               
               
                   
               
               
                 1261 
                 gctcccatgc catcttcatc atcactgtgg agtgcagcga acgtggctct gatggccagg 
               
               
                   
               
               
                 1321 
                 accacatccg agtgggcaag ctcaacctcg tggacctggc tggcagcgag aggcagaaca 
               
               
                   
               
               
                 1381 
                 aggcaggccc caacacagcg ggaggggcag ccacaccatc ctcgggtggc ggtggtggcg 
               
               
                   
               
               
                 1441 
                 gtggaggcag tggtggtggt gctggtggag agaggcctaa ggaagcctcc aaaatcaacc 
               
               
                   
               
               
                 1501 
                 tctcattatc tgccctgggc aacgtgattg ctgccctggc gggcaacagg agcacccaca 
               
               
                   
               
               
                 1561 
                 ttccctaccg ggactccaag ctgacccggc tgctccagga ctccctgggg gggaatgcca 
               
               
                   
               
               
                 1621 
                 agaccatcat ggtagccaca ctggggccag cttctcacag ctacgatgag agcctctcca 
               
               
                   
               
               
                 1681 
                 ccttgcgctt tgccaaccga gccaagaaca tcaagaacaa gccccgggtg aacgaggacc 
               
               
                   
               
               
                 1741 
                 ccaaggacac actgctgcgg gaattccaag aggagattgc ccgcctgaag gcccagctgg 
               
               
                   
               
               
                 1801 
                 agaagagggg gatgctgggg aagcggcccc ggaggaagag cagccgcagg aagaaggccg 
               
               
                   
               
               
                 1861 
                 tgtccgcccc gcctgggtac cctgagggcc cagtgattga ggcctgggtg gcagaagagg 
               
               
                   
               
               
                 1921 
                 aggatgacaa caacaacaac caccgcccgc cccagcccat cctggagtca gccttggaga 
               
               
                   
               
               
                 1981 
                 agaacatgga gaattacctg caggaacaga aggagcggct ggaggaggag aaggcagcca 
               
               
                   
               
               
                 2041 
                 tccaggatga ccgcagcctg gtgagcgagg agaagcagaa gctgctggag gagaaggaga 
               
               
                   
               
               
                 2101 
                 agatgctgga ggacctgcgg cgggaacagc aggccacaga gctgcttgcg gccaagtaca 
               
               
                   
               
               
                 2161 
                 aggccatgga gagcaagctc ctcatcgggg gcaggaacat catggatcac accaacgaac 
               
               
                   
               
               
                 2221 
                 agcagaagat gttggaactg aagaggcagg agattgccga gcagaaacgt cgtgagcggg 
               
               
                   
               
               
                 2281 
                 agatgcagca ggagatgatg ctccgggacg aggagactat ggagctccgg ggcacctaca 
               
               
                   
               
               
                 2341 
                 catccctgca gcaggaggtg gaggtcaaaa ccaagaaact caagaagctc tacgccaagc 
               
               
                   
               
               
                 2401 
                 tgcaggcggt gaaggcggag atccaggacc agcatgatga gtatatccgc gtgcggcagg 
               
               
                   
               
               
                 2461 
                 acctggagga ggcgcagaac gagcagaccc gcgaactcaa gctcaagtac ctaatcatcg 
               
               
                   
               
               
                 2521 
                 agaacttcat cccgccggag gagaagaaca agatcatgaa ccggcttttc ctggactgtg 
               
               
                   
               
               
                 2581 
                 aggaggagca gtggaagttc cagccactgg tgccagccgg cgtcagtagc agccagatga 
               
               
                   
               
               
                 2641 
                 agaagcggcc aacatctgca gtgggctaca agaggcctat cagccagtat gctcgggttg 
               
               
                   
               
               
                 2701 
                 ccatggcaat ggggtcccac cccaggtaca gggctgaaaa cataatgttt ctggagttgg 
               
               
                   
               
               
                 2761 
                 atgtgtcccc tccagctgtc tttgagatgg aattctctca cgaccaagaa caagaccctc 
               
               
                   
               
               
                 2821 
                 gtgcgctaca catggagagg ctcatgcgat tggacagctt tctggaaaga ccttccacgt 
               
               
                   
               
               
                 2881 
                 ctaaagtccg aaagtccaga tcctggtgcc agagtcctca gcggcctcca ccttccacca 
               
               
                   
               
               
                 2941 
                 cacatgcctc cctggcctct gcttctctgc gccctgcaac agtggcggac catgagtgac 
               
               
                   
               
               
                 3001 
                 aaccatcacg tcaggctgcc catccaatag actcctggga tggggcagcc aaccctggct 
               
               
                   
               
               
                 3061 
                 catctcatct gccgcttggt gcgtgtgcgt gtgcgtgcat gtgcgtgtgc gtgtgtgcag 
               
               
                   
               
               
                 3121 
                 gggtgagaat ctggcagatg gtgcctctgc ctgctcttct tcgcctcctt tatttaattc 
               
               
                   
               
               
                 3181 
                 atgttattta ttcgcggagc tctgttcgtg ttggggagat gccctcgcct gagccgtctg 
               
               
                   
               
               
                 3241 
                 ggcctaccgt ggtcactgcg tagcctcttt ttcttctgac ttgagagctc ccccagtcag 
               
               
                   
               
               
                 3301 
                 atctcaggct tgtccccctg tcagctgcct ccagaaggga aggtagccag tgcctgagaa 
               
               
                   
               
               
                 3361 
                 gacagtccct tttctaccca ccgcactcca taacctccat cttctcccac actgatggcg 
               
               
                   
               
               
                 3421 
                 agcagcccct gagcactttc tgggactggg agactgcttg gtgttccctg aggacaagag 
               
               
                   
               
               
                 3481 
                 acatcctgac agtgttgggc atctgctccc cgtggacaca gccccactct ccactttctg 
               
               
                   
               
               
                 3541 
                 agcctcagac aacctcattc agcctcttgg gctccttttc aaggacatta ataacctcac 
               
               
                   
               
               
                 3601 
                 caacatagct catgcccttc agctttgaca agaactcacg gcttcccaaa ctctgctttc 
               
               
                   
               
               
                 3661 
                 tgcccacctt ggatgggaac tgtggaccaa gcaattacca tcgccttgga acctgcagga 
               
               
                   
               
               
                 3721 
                 aatggaacag caattgagac aacttgaaca gtcatcaacg gaagtccctc cactggattc 
               
               
                   
               
               
                 3781 
                 ctttgtttct gtcccctccg aggagtcatt ttggtcgaca ggctctcaag gcaactcccc 
               
               
                   
               
               
                 3841 
                 attttcaaga ggctgctcct gcctgcttcg atcatttctc cctgcagctg cctagacccc 
               
               
                   
               
               
                 3901 
                 gttcacagtg ggaggagtca atgtcattct acccctcgct aaacgaagat attaacatct 
               
               
                   
               
               
                 3961 
                 attgcttttt cccttcatct gtcacaggaa acagaagccc aggcacaatc ttttccagct 
               
               
                   
               
               
                 4021 
                 ttgcctgtta cccctgtttc tgaattgcat ctttaaggta ttattttgtt gacaatagat 
               
               
                   
               
               
                 4081 
                 cctttattca ctagttacgc aaattggttc ctagggggat actccttacc ttcctttgtg 
               
               
                   
               
               
                 4141 
                 atggcccaaa atgtctctag gtatctcaag tgataagtaa atttctacaa aaaaaaatgg 
               
               
                   
               
               
                 4201 
                 ttaatgttca ttgactggct ttttaagtgt atattttgga ggacgggtga agaggtcata 
               
               
                   
               
               
                 4261 
                 acgaaagcaa gcgagtgaat taggatttca aagtgcccta atagtgtgag tctccagttc 
               
               
                   
               
               
                 4321 
                 ctagaatatg aagagtgctg tcgttggggt gaaaccatga gactgacaga tctgcctgaa 
               
               
                   
               
               
                 4381 
                 atggggggtg tgggaggtgg tggcgggggt tattctcttt ccttcaggaa atgaaccctt 
               
               
                   
               
               
                 4441 
                 cttacatcat tcaagttctg ctctgaggat caagcttggg tctgatttaa ctcagcgaca 
               
               
                   
               
               
                 4501 
                 ctgtcatttc tgcttcatta ctggactaga gggttgagcc acccacttgc catttgctcc 
               
               
                   
               
               
                 4561 
                 tgtccttcca ggaaatcaca attttcatca gagcccaaga gattatttga gactcaggat 
               
               
                   
               
               
                 4621 
                 tcagatcaga ggttcgactg tggctgggac aggagttgtg tgtagaaatt caccaggtgg 
               
               
                   
               
               
                 4681 
                 cctgagcgca gggggacctc cagggctgcg ttgagcagcc tctcccactg acctctttct 
               
               
                   
               
               
                 4741 
                 cgtttgtgga caaagcagca cgtatcacct cattcatcac ttggacacat cgcctttgca 
               
               
                   
               
               
                 4801 
                 ttgtcttgtc acacctccct cacagtctta tagcacaata tacccaaatc agccccccca 
               
               
                   
               
               
                 4861 
                 gtccgagggc tgggcccaag gtatggtcgg aggaggagct cctgcctgcg gttttgtgta 
               
               
                   
               
               
                 4921 
                 tgtgtgtatg tgtgtgcgtg tttgtgtgcg tgtttacctc cacaggggac actctacact 
               
               
                   
               
               
                 4981 
                 cagtgtaaga tctgctggga acagggccac caggagtggc tggatctcag tctctctgtc 
               
               
                   
               
               
                 5041 
                 tctctttctc tccttttcct tttggtgtat caaatatttg attgacaaag taagggcctt 
               
               
                   
               
               
                 5101 
                 gattaggacc aaattctcgt gtgttgctat ggtctttatt taggacaaca attaacaatg 
               
               
                   
               
               
                 5161 
                 cagtggccca ttcttgtcac tctacacata tgactatacg ggacatatgt aatatataaa 
               
               
                   
               
               
                 5221 
                 tatatatata aaacattccc ctctgtcccc ttggcttcgg atggaggcct ttctgttgag 
               
               
                   
               
               
                 5281 
                 ctgaaatgca cctgcagctg ggtgctgcca gcagcttgca ggccccagcc ctgttccaat 
               
               
                   
               
               
                 5341 
                 caatgcagtt gacaataaag gaatgagtat cgtcacggaa aaaaaaaaaa aaaaaaaaaa 
               
               
                   
               
               
                 5401 
                 a 
               
               
                   
               
             
          
           
               
                 25: BQ135232   CD9 
                   
               
             
          
           
               
                 1 
                 taaacaatgg tatcaacgca aagtaagcgg gcagccgcct gcatctgtat ccagcgccag 
                   
               
               
                   
               
               
                 61 
                 gtcccgccag tcccagtgcg cgcgcccccc agtcccgcac ccgttcggcc aggctaagtt 
               
               
                   
               
               
                 121 
                 agccctcacc atgcggtcaa aggaggcagc aagtgcatca aatacctgct gttcggattt 
               
               
                   
               
               
                 181 
                 aacttcatct tctggcttgc cgggattgct gtccttgcca ttggactatg ggtccgattc 
               
               
                   
               
               
                 241 
                 gactctcaga ccaagagcat cttcgagcaa gaaatttcct aataataata attccagctt 
               
               
                   
               
               
                 301 
                 ctacacagga gtctatattc tgatcggagc cggcgccctc atgatgctgg tgggcttcct 
               
               
                   
               
               
                 361 
                 gggctgctgc ggggctgtgc aggagtccca gtgcatgctg ggactgttct tcggcttcct 
               
               
                   
               
               
                 421 
                 cttggtgata ttcgccattg aaatagctgc ggccatctgg ggatattccc acaaggatga 
               
               
                   
               
               
                 481 
                 ggtgattaag gaagtccagg agttttacaa ggacacctac aacaagctga aaaccaagga 
               
               
                   
               
               
                 541 
                 tgagccccag cgggaaacgc tgaaagccat ccactatgcg ttgaactgct gtggtttggc 
               
               
                   
               
               
                 601 
                 tgggggcgtg gaacagttta tctcagacat ctgccccaag aaggacgtac tcgaaacctt 
               
               
                   
               
               
                 661 
                 caccgtgaag tcctgtcctg atgccatcaa agaggtcttc gaccaataaa ttccacatca 
               
               
                   
               
               
                 721 
                 tcggcgcagt gggcatcggc attgccgtgg tcatgatatt tggcatgatc ttcagtatga 
               
               
                   
               
               
                 781 
                 tcttgtgctg tgctatccgc aggaaccgcg agatggtcta gagtcagctt acatccctga 
               
               
                   
               
               
                 841 
                 gcaggaaagt ttacccatga agattggtgg gattttttgt ttgtttgttt tgttttgttt 
               
               
                   
               
               
                 901 
                 gttgtttgtt gtttgttttt ttgccactaa ttttagtatt cattctgcat tgctagataa 
               
               
                   
               
               
                 961 
                 aagctgaagt tactttatgt ttgtctttta atgcttcatt caatattgac atttgtagtt 
               
               
                   
               
               
                 1021 
                 gagcgggggg tttggtttgc tttggtttat attttttcag ttgtttgttt ttgcttgtta 
               
               
                   
               
               
                 1081 
                 tattaagcag aaatcctgca atgaaaggta ctatatttgc tagactctag acaagatatt 
               
               
                   
               
               
                 1141 
                 gtacataaaa gaattttttt gtctttaaat agatacaaat gtctatcaac tttaatcaag 
               
               
                   
               
               
                 1201 
                 ttgtaactta tattgaagac aatttgatac ataataaaaa attatgacaa tggccaaaaa 
               
               
                   
               
               
                 1261 
                 aaaaaaaaaa aaaaaaaaaa 
               
               
                   
               
             
          
           
               
                 26: BC051322   LRRC8 
                   
               
             
          
           
               
                 1 
                 gcggccgggg cctggggctg cctgccgggc ggccgggcgc ggcgagccca gggaggcagc 
                   
               
               
                   
               
               
                 61 
                 gtccatggag caaaaggaat gccaggatcc tgcacaggca gacgcgggcc agcctcagca 
               
               
                   
               
               
                 121 
                 ccgacagccg acgcgcagat agcagagcca tccttggggt tgaaccatga ttccggtgac 
               
               
                   
               
               
                 181 
                 agagctccgc tactttgcgg acacgcagcc agcataccgg atcctgaagc cgtggtggga 
               
               
                   
               
               
                 241 
                 tgtgttcaca gactacatct ctatcgtcat gctgatgatt gccgtcttcg gggggacgct 
               
               
                   
               
               
                 301 
                 gcaggtcacc caagacaaga tgatctgcct gccttgtaag tgggtcacca aggactcctg 
               
               
                   
               
               
                 361 
                 caatgattcg ttccggggct gggcagcccc tggcccggag cccacctacc ccaactccac 
               
               
                   
               
               
                 421 
                 cattctgccg acccctgaca cgggccccac aggcatcaag tatgacctgg accggcacca 
               
               
                   
               
               
                 481 
                 gtacaactac gtggacgctg tgtgctatga gaaccgactg cactggtttg ccaagtactt 
               
               
                   
               
               
                 541 
                 cccctacctg gtgcttctgc acacgctcat cttcctggcc tgcagcaact tctggttcaa 
               
               
                   
               
               
                 601 
                 attcccgcgc accagctcga agctggagca ctttgtgtct atcctgctga agtgcttcga 
               
               
                   
               
               
                 661 
                 ctcgccctgg accacgaggg ccctgtcgga gacagtggtg gaggagagcg accccaagcc 
               
               
                   
               
               
                 721 
                 ggccttcagc aagatgaatg ggtccatgga caaaaagtca tcgaccgtca gtgaggacgt 
               
               
                   
               
               
                 781 
                 ggaggccacc gtgcccatgc tgcagcggac caagtcacgg atcgagcagg gtatcgtgga 
               
               
                   
               
               
                 841 
                 ccgctcagag acgggcgtgc tggacaagaa ggagggggag caagccaagg cgctgtttga 
               
               
                   
               
               
                 901 
                 gaaggtgaag aagttccgga cccatgtgga ggagggggac attgtgtacc gcctctacat 
               
               
                   
               
               
                 961 
                 gcggcagacc atcatcaagg tgatcaagtt catcctcatc atctgctaca ccgtctacta 
               
               
                   
               
               
                 1021 
                 cgtgcacaac atcaagttcg acgtggactg caccgtggac attgagagcc tgacgggcta 
               
               
                   
               
               
                 1081 
                 ccgcacctac cgctgtgccc accccctggc cacactcttc aagatcctgg cgtccttcta 
               
               
                   
               
               
                 1141 
                 catcagccta gtcatcttct acggcctcat ctgcatgtat acactgtggt ggatgctacg 
               
               
                   
               
               
                 1201 
                 gcgctccctc aagaagtact cgtttgagtc gatccgtgag gagagcagct acagcgacat 
               
               
                   
               
               
                 1261 
                 ccccgacgtc aagaacgact tcgccttcat gctgcacctc attgaccaat acgacccgct 
               
               
                   
               
               
                 1321 
                 ctactccaag cgcttcgccg tcttcctgtc ggaggtgagt gagaacaagc tgcggcagct 
               
               
                   
               
               
                 1381 
                 gaacctcaac aacgagtgga cgctggacaa gctccggcag cggctcacca agaacgcgca 
               
               
                   
               
               
                 1441 
                 ggacaagctg gagctgcacc tgttcatgct cagtggcatc cctgacactg tgtttgacct 
               
               
                   
               
               
                 1501 
                 ggtggagctg gaggtcctca agctggagct gatccccgac gtgaccatcc cgcccagcat 
               
               
                   
               
               
                 1561 
                 tgcccagctc acgggcctca aggagctgtg gctctaccac acagcggcca agattgaagc 
               
               
                   
               
               
                 1621 
                 gcccgcgctg gccttcctgc gcgagaacct gcgggcgctg cacatcaagt tcaccgacat 
               
               
                   
               
               
                 1681 
                 caaggagatc ccgctgtgga tctatagcct gaagacactg gaggagctgc acctgacggg 
               
               
                   
               
               
                 1741 
                 caacctgagc gcggagaaca accgctacat cgtcatcgac gggctgcggg agctcaaacg 
               
               
                   
               
               
                 1801 
                 cctcaaggtg ctgcggctca agagcaacct aagcaagctg ccacaggtgg tcacagatgt 
               
               
                   
               
               
                 1861 
                 gggcgtgcac ctgcagaagc tgtccatcaa caatgagggc accaagctca tcgtcctcaa 
               
               
                   
               
               
                 1921 
                 cagcctcaag aagatggcga acctgactga gctggagctg atccgctgtg acctggagcg 
               
               
                   
               
               
                 1981 
                 catcccccac tccatcttca gcctccacaa cctgcaggag attgacctca aggacaacaa 
               
               
                   
               
               
                 2041 
                 cctcaagacc atcgaggaga tcatcagctt ccagcacctg caccgcctca cctgccttaa 
               
               
                   
               
               
                 2101 
                 gctgtggtac aaccacatcg cctacatccc catccagatc ggcaacctca ccaacctgga 
               
               
                   
               
               
                 2161 
                 gcgcctctac ctgaaccgca acaagatcga gaagatcccc acccagctct tctactgccg 
               
               
                   
               
               
                 2221 
                 caagctgcgc tacctggacc tcagccacaa caacctgacc ttcctccctg ccgacatcgg 
               
               
                   
               
               
                 2281 
                 cctcctgcag aacctccaga acctagccat cacggccaac cggatcgaga cgctccctcc 
               
               
                   
               
               
                 2341 
                 ggagctcttc cagtgccgga agctgcgggc cctgcacctg ggcaacaacg tgctgcagtc 
               
               
                   
               
               
                 2401 
                 actgccctcc agggtgggcg agctgaccaa cctgacgcag atcgagctgc ggggcaaccg 
               
               
                   
               
               
                 2461 
                 gctggagtgc ctgcctgtgg agctgggcga gtgcccactg ctcaagcgca gcggcttggt 
               
               
                   
               
               
                 2521 
                 ggtggaggag gacctgttca acacactgcc acccgaggtg aaggagcggc tgtggagggc 
               
               
                   
               
               
                 2581 
                 tgacaaggag caggcctgag cgaggccggc ccagcacagc aagcagcagg accgctgccc 
               
               
                   
               
               
                 2641 
                 agtcctcagg cccggagggg caggcctagc ttctcccaga actcccggac agccaggaca 
               
               
                   
               
               
                 2701 
                 gcctcgtggc tgggcaggag cctggggccg cttgtgagtc aggccagagc gagaggacag 
               
               
                   
               
               
                 2761 
                 tatctgtggg gctggcccct tttctccctc tgagactcac gtcccccagg gcaagtgctt 
               
               
                   
               
               
                 2821 
                 gtggaggaga gcaagtctca agagcgcagt atttggataa tcagggtctc ctccctggag 
               
               
                   
               
               
                 2881 
                 gccagctctg ccccaggggc tgagctgcca ccagaggtcc tgggaccctc actttagttc 
               
               
                   
               
               
                 2941 
                 ttggtattta tttttctcca tctcccacct ccttcatcca gataacttat acattcccaa 
               
               
                   
               
               
                 3001 
                 gaaagttcag cccagatgga aggtgttcag ggaaaggtgg gctgcctttt ccccttgtcc 
               
               
                   
               
               
                 3061 
                 ttatttagcg atgccgccgg gcatttaaca cccacctgga cttcagcaga gtggtccggg 
               
               
                   
               
               
                 3121 
                 gcgaaccagc catgggacgg tcacccagca gtgccgggct gggctctgcg gtgcggtcca 
               
               
                   
               
               
                 3181 
                 cgggagagca ggcctccagc tggaaaggcc aggcctggag cttgcctctt cagtatttgt 
               
               
                   
               
               
                 3241 
                 ggcagtttta gttttttgtt tttttttttt taatcaaaaa acaatttttt taaaaaaaaa 
               
               
                   
               
               
                 3301 
                 gctttgaaaa tggatggttt gggtattaaa aaaaaaaaaa aaaaa 
               
               
                   
               
             
          
           
               
                 27: BC038504   SNF1LK 
                   
               
             
          
           
               
                 1 
                 atgcggcgcg gccccggagg cagcagcagc ggcggcggca gccggagcag taggcacccg 
                   
               
               
                   
               
               
                 61 
                 agcagcgcca gcggccgagc gggcggcttc ctggcctggg cgctccggtg gcggcggagg 
               
               
                   
               
               
                 121 
                 tgcgcgcgga gccatggtta tcatgtcgga gttcagcgcg gaccccgcgg gccagggtca 
               
               
                   
               
               
                 181 
                 gggccagcag aagcccctcc gggtgggttt ttacgacatc gagcggaccc tgggcaaagg 
               
               
                   
               
               
                 241 
                 caacttcgcg gtggtgaagc tggcgcggca tcgagtcacc aaaacgcagg ttgcaataaa 
               
               
                   
               
               
                 301 
                 aataattgat aaaacacgat tagattcaag caatttggag aaaatctatc gtgaggttca 
               
               
                   
               
               
                 361 
                 gctgatgaag cttctgaacc atccacacat cataaagctt taccaggtta tggaaacaaa 
               
               
                   
               
               
                 421 
                 ggacatgctt tacatcgtca ctgaatttgc taaaaatgga gaaatgtttg attatttgac 
               
               
                   
               
               
                 481 
                 ttccaacggg cacctgagtg agaacgaggc gcggaagaag ttctggcaaa tcctgtcggc 
               
               
                   
               
               
                 541 
                 cgtggagtac tgtcacgacc atcacatcgt ccaccgggac ctcaagaccg agaacctcct 
               
               
                   
               
               
                 601 
                 gctggatggc aacatggaca tcaagctggc agattttgga tttgggaatt tctacaagtc 
               
               
                   
               
               
                 661 
                 aggagagcct ctgtccacgt ggtgtgggag ccccccgtat gccgccccgg aagtctttga 
               
               
                   
               
               
                 721 
                 ggggaaggag tatgaaggcc cccagctgga catctggagc ctgggcgtgg tgctgtacgt 
               
               
                   
               
               
                 781 
                 cctggtctgc ggttctctcc ccttcgatgg gcctaacctg ccgacgctga gacagcgggt 
               
               
                   
               
               
                 841 
                 gctggagggc cgcttccgca tccccttctt catgtctcaa gactgtgaga gcctgatccg 
               
               
                   
               
               
                 901 
                 ccgcatgctg gtggtggacc ccgccaggcg catcaccatc gcccagatcc ggcagcaccg 
               
               
                   
               
               
                 961 
                 gtggatgcgg gctgagccct gcttgccggg acccgcctgc cccgccttct ccgcacacag 
               
               
                   
               
               
                 1021 
                 ctacacctcc aacctgggcg actacgatga gcaggcgctg ggtatcatgc agaccctggg 
               
               
                   
               
               
                 1081 
                 cgtggaccgg cagaggacgg tggagtcact gcaaaacagc agctataacc actttgctgc 
               
               
                   
               
               
                 1141 
                 catttattac ctcctccttg agcggctcaa ggagtatcgg aatgcccagt gcgcccgccc 
               
               
                   
               
               
                 1201 
                 cgggcctgcc aggcagccgc ggcctcggag ctcggacctc agtggtttgg aggtgcctca 
               
               
                   
               
               
                 1261 
                 ggaaggtctt tccaccgacc ctttccgacc tgccttgctg tgcccgcagc cgcagacctt 
               
               
                   
               
               
                 1321 
                 ggtgcagtcc gtcctccagg ccgagatgga ctgtgagctc cagagctcgc tgcagtggcc 
               
               
                   
               
               
                 1381 
                 cttgttcttc ccggtggatg ccagctgcag cggagtgttc cggccccggc ccgtgtcccc 
               
               
                   
               
               
                 1441 
                 aagcagcctg ctggacacag ccatcagtga ggaggccagg caggggccgg gcctagagga 
               
               
                   
               
               
                 1501 
                 ggagcaggac acgcaggagt ccctgcccag cagcacgggc cggaggcaca ccctggccga 
               
               
                   
               
               
                 1561 
                 ggtctccacc cgcctctccc cactcaccgc gccatgtata gtcgtctccc cctccaccac 
               
               
                   
               
               
                 1621 
                 ggcaagtcct gcagagggaa ccagctctga cagttgtctg accttctctg cgagcaaaag 
               
               
                   
               
               
                 1681 
                 ccccgcgggg ctcagtggca ccccggccac tcaggggctg ctgggcgcct gctccccggt 
               
               
                   
               
               
                 1741 
                 caggctggcc tcgcccttcc tggggtcgca gtccgccacc ccagtgctgc aggctcaggg 
               
               
                   
               
               
                 1801 
                 gggcttggga ggagctgttc tgctccctgt cagcttccag gagggacggc gggcgtcgga 
               
               
                   
               
               
                 1861 
                 cacctcactg actcaagggc tgaaggcctt tcggcagcag ctgaggaaga ccacgcggac 
               
               
                   
               
               
                 1921 
                 caaagggttt ctgggactga acaaaatcaa ggggctggct cgccaggtgt gccaggtccc 
               
               
                   
               
               
                 1981 
                 tgccagccgg gccagcaggg gcggcctgag ccccttccac gcccctgcac agagcccagg 
               
               
                   
               
               
                 2041 
                 cctgcacggc ggcgcagccg gcagccggga gggctggagc ctgctggagg aggtgctaga 
               
               
                   
               
               
                 2101 
                 gcagcagagg ctgctccagt tacagcacca cccggccgct gcacccggct gctcccaggc 
               
               
                   
               
               
                 2161 
                 cccccagccg gcccctgccc cgtttgtgat cgccccctgt gatggccctg gggctgcccc 
               
               
                   
               
               
                 2221 
                 gctccccagc accctcctca cgtcggggct cccgctgctg ccgcccccac tcctgcagac 
               
               
                   
               
               
                 2281 
                 cggcgcgtcc ccggtggcct cagcggcgca gctcctggac acacacctgc acattggcac 
               
               
                   
               
               
                 2341 
                 cggccccacc gccctccccg ctgtgccccc accacgcctg gccaggctgg ccccaggttg 
               
               
                   
               
               
                 2401 
                 tgagcccctg gggctgctgc agggggactg tgagatggag gacctgatgc cctgctccct 
               
               
                   
               
               
                 2461 
                 aggcacgttt gtcctggtgc agtgagggca gccctgcatc ctggcacgga cactgactct 
               
               
                   
               
               
                 2521 
                 tacagcaata acttcagagg aggtgaagac atctggcctc aaagccaaga actttctaga 
               
               
                   
               
               
                 2581 
                 agcgaaataa gcaatacgtt aggtgttttg gctttttagt ttatttttgt tttatttttt 
               
               
                   
               
               
                 2641 
                 tcttgcactg agtgacctca actttgagta gggactggaa actttaggaa gaaagataat 
               
               
                   
               
               
                 2701 
                 tgaggggcgt gtctgggggc gggggcagga ggggagcggg gtggagggaa cacgtgcagt 
               
               
                   
               
               
                 2761 
                 gccgtggtgt ggggatctcg gcccctctct ctgggttcgt cgtggttgag atgattacct 
               
               
                   
               
               
                 2821 
                 cggacgtcta cggaaacgag cgggcgcatt gttgtccgct tgtgtgtgtg tgtgtgtgtg 
               
               
                   
               
               
                 2881 
                 tgtgtgtgcg cgtgcattga ttactatcca tttctttagt caacgctctc cacttcctga 
               
               
                   
               
               
                 2941 
                 tttctgcttt aaggaaaact gtgaactttc tgcttcatgt atcagtttta aagcagccca 
               
               
                   
               
               
                 3001 
                 ggcaaagatc atctacagat tctaggaatt ctctcccctg aaatcaaaac ctggaagact 
               
               
                   
               
               
                 3061 
                 tttttttctt attttagttg agaagtttca taaactgctc aaggattagt tttccaggac 
               
               
                   
               
               
                 3121 
                 tctgcggagg aacggcagga agaacctcag agagggcaga ggtgacttca aagtgctggg 
               
               
                   
               
               
                 3181 
                 gactccgtcc tgagggtcac ttggccctga gcccctgcgt gcccttgcgg aagcccagaa 
               
               
                   
               
               
                 3241 
                 gcttcttcct gctgcacctc ccgtttccgc tgctgctgac gtttatgcat ttcatgatgg 
               
               
                   
               
               
                 3301 
                 ggtccaacaa gaacacctga cttgggtgaa gttgtgcaat attggaggct gactgtaggg 
               
               
                   
               
               
                 3361 
                 ctgggcagct gggagacagg ctcatggctc atggctcatg gctcagggcg gtgcctgcca 
               
               
                   
               
               
                 3421 
                 tgggccggga cccccctccc caccccccac ctaggctttt tgggttttgt tcaaggaagg 
               
               
                   
               
               
                 3481 
                 taaagtgaga ggtttaggtc agtgttttta agtttttgtt ttttttttaa agcaaatcct 
               
               
                   
               
               
                 3541 
                 gtatatgtat ctacatggga gacaggtaga cactacttat ttgttacatt ttgtactaca 
               
               
                   
               
               
                 3601 
                 cgtttgtgtt ccaggtttca gcttccctcg ctcctgttgt taagaagcgt ccctgtcagc 
               
               
                   
               
               
                 3661 
                 acaggtgtgc attgaggaag gggccccagg gccttcgctc cctcagcact ggggtggagg 
               
               
                   
               
               
                 3721 
                 cggcaggaag gggcggccct tacctggcag gtctgggcgc acctttagca ggtggactcc 
               
               
                   
               
               
                 3781 
                 gtggggctcc accagccaga agcctttgga aggcaacgaa ggcaatgctg ctccctgagt 
               
               
                   
               
               
                 3841 
                 ccagtccccg cccccaaacc cagcccaggt gccttcagct acttcggctt cttaaaccct 
               
               
                   
               
               
                 3901 
                 gcagtgttaa acagaggcat tgagaaaggg gaaaggcggg tatttttaaa agccaaagat 
               
               
                   
               
               
                 3961 
                 tgacccagtt acttgagggt agggaggcgg gcccagtgca ggaggctgca tccctggcct 
               
               
                   
               
               
                 4021 
                 gctggtgccc accgggggct gtgcctgtgc cgggccgcag ggaagctggc tgcccccatt 
               
               
                   
               
               
                 4081 
                 cctgctgctg ctgctgctgc tgctctgtgg ctgtttcaaa gactgggcga aaggctgtcc 
               
               
                   
               
               
                 4141 
                 ggagggcaga ccaggtgcct tgccgcagag aaaacaccaa agtctcctgt tcgctcataa 
               
               
                   
               
               
                 4201 
                 agaagttttt gggatgggag agaatccaga ccatcttggg gcagccaggc ccttgccttc 
               
               
                   
               
               
                 4261 
                 atttttacag aggtagcaca attgattcca acacaaaact tccccttttt aaaatgattt 
               
               
                   
               
               
                 4321 
                 ctgttctaat gccatagatc aaaggcctca gaaaccattg tgtgtttcct ctttgaagca 
               
               
                   
               
               
                 4381 
                 atgacaagca ctttactttc acggtggttt ttgttttttc ttattgctgt ggaacctctt 
               
               
                   
               
               
                 4441 
                 ttggaggacg ttaaaggcgt gttttacttg tttttttaag agtgtgtgat gtgtgttttg 
               
               
                   
               
               
                 4501 
                 tagatttctt gacagtgctg taatacagac ggcaatgcaa tagcctattt aaagacacta 
               
               
                   
               
               
                 4561 
                 cgtgatctga ttgagatgta catagttttt ttttttacca taactgaatt attttatctc 
               
               
                   
               
               
                 4621 
                 ttatgttaac atgagaaatg tatgccaaat gattagttga tgtatgtttt ttaatttaat 
               
               
                   
               
               
                 4681 
                 atttaaataa aatatttggg agtataaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
               
               
                   
               
               
                 4741 
                 aaaaaaaaaa aaaaaaaaaa aa 
               
               
                   
               
             
          
           
               
                 28: U78556   CRA 
                   
               
             
          
           
               
                 1 
                 cacacctttc caaggacccc caaactctgc tccgtgcacg tcaaatgctc ctttcccttg 
                   
               
               
                   
               
               
                 61 
                 tgtccaaccc cctacccctc tccctaacac ccctcttctc aacaagactc agcctctccc 
               
               
                   
               
               
                 121 
                 cgaggtgggt gagcatcctt gaggtttccc acccttaact gctgtgtccc cggatggagc 
               
               
                   
               
               
                 181 
                 cagagaaatg tggtgggggg gccggggcag agtttcaaca ttgcccccca gaaggaggag 
               
               
                   
               
               
                 241 
                 ccagagatgg ggtctgtcca ggaaaacagg atgccggagc ccaggagtcg tcagcctagc 
               
               
                   
               
               
                 301 
                 agttgcctgg cctccagatg cctcccaggg gagcagatcc tagcatgggc cccaggggtg 
               
               
                   
               
               
                 361 
                 aggaagggcc tggaaccaga attgtctgga accctgatct gtaccaactt tagggtcacc 
               
               
                   
               
               
                 421 
                 ttccagccct gtggatggca gtggaatcag gacactccct tgaacagtga atacgatttt 
               
               
                   
               
               
                 481 
                 gccctggtca acattggacg attagaggct gtgagcggct tgtcccgagt ccagctcctc 
               
               
                   
               
               
                 541 
                 cgtccagggt ccctgcataa atttatccct gaggagattc tgattcatgg ccgagacttc 
               
               
                   
               
               
                 601 
                 cggctgctca gagttggttt tgaggctgga ggcctagagc ctcaggcttt tcaggtgacc 
               
               
                   
               
               
                 661 
                 atggccattg tccaagccag agctcagagc aatcaagccc aacagtattc ggggataacc 
               
               
                   
               
               
                 721 
                 ctgagcaagg ctggccaggg ttctggctcc agaaaaccac caattcctct catggagaca 
               
               
                   
               
               
                 781 
                 gcggaagact gggagactga gcggaagaag caggcagcca gaggctggag ggtcagcacg 
               
               
                   
               
               
                 841 
                 gtcaacgaga ggttcgacgt agccaccagc ctcccccgtt acttctgggt ccctaaccga 
               
               
                   
               
               
                 901 
                 attctggaca gtgaggtcag gagagcattt ggccactttc atcagggccg tggaccggtc 
               
               
                   
               
               
                 961 
                 agtgtgatgg ttagggtaat ggctgtggat tagagggtca tgtgggccag ggacatcgtg 
               
               
                   
               
               
                 1021 
                 gagggaggaa cctctgtgag gtcagtgtgg gggcaagggt agcgtggagc taggcatttc 
               
               
                   
               
               
                 1081 
                 tcccacaatg accctcttct gccccatgtg aagcgcttgt cctggcatca ccctgggggc 
               
               
                   
               
               
                 1141 
                 agtgatcttc tccgctgtgg aggcttctat acagccagtg accctaacaa ggaggatatc 
               
               
                   
               
               
                 1201 
                 agagcagtgg agttgatgca ccaggctggg cattcagatg ttgtcctggt agacactatg 
               
               
                   
               
               
                 1261 
                 gatgagctgc ccagccttgc agatgtccaa cttgcccacc tgaggctgag ggccctctgc 
               
               
                   
               
               
                 1321 
                 ctgcctgatt catctgtagc tgaggataaa tgctttcagc cctggaagga acacgatggc 
               
               
                   
               
               
                 1381 
                 tggactatgt cagggcttgt cttcgaaagg ccagtgacat ttcagtatta gtgacatcca 
               
               
                   
               
               
                 1441 
                 gggttcgttc tgtaatactt caaggctccg gtgtttctcc tcttccttga ttgtgtctgg 
               
               
                   
               
               
                 1501 
                 cagctcctcc agcagtttcc agctgatttt gaattctctg agtttttcct tcttgctctt 
               
               
                   
               
               
                 1561 
                 catgacagtg tcagggttcc tgacaccctt accttcctga gaaatacccc ctgggagcgc 
               
               
                   
               
               
                 1621 
                 ggaaagcaga gcggacaggt cagtgacttc tatttttgac tcgtgttttt ttttccattg 
               
               
                   
               
               
                 1681 
                 agatgtactc tctgaagttt ggtcttgatt tgttttatga gaagtgaggt ctgtgagtgg 
               
               
                   
               
               
                 1741 
                 ggagggggag atttattctc attttcagga cgagactttt gccctacatc tttcctagaa 
               
               
                   
               
               
                 1801 
                 taagaggtga gaatctcatg atttgtctct agatgtggga ggattgtgtg taaccatcct 
               
               
                   
               
               
                 1861 
                 ttttcttgct tcctctgtcc agttaaactc ctatacacaa gtctacaccc caggatactc 
               
               
                   
               
               
                 1921 
                 cagcctccag ctgggaactc ttttaacctg cagctgtctg tctgggactg ggatttacgt 
               
               
                   
               
               
                 1981 
                 tatagcaatg cacagatact acaattccag aatcctggct atgacccaga acactgtcca 
               
               
                   
               
               
                 2041 
                 gattcctggc tccctagacc acagccaagc ttcatggttc ctggaccccc cagttttgtg 
               
               
                   
               
               
                 2101 
                 tggctcttct ctagaggagc attgaccccc ctgaatcagc tctgtccttg gcgggacagt 
               
               
                   
               
               
                 2161 
                 ccttccctgc tggcagtctc ttctcgttgg ctccctcgac ctgctatctc ctctgaaagc 
               
               
                   
               
               
                 2221 
                 tggctgacca ggaatggggt ctcccctcac attggggagc ttgcccttta cctccagggc 
               
               
                   
               
               
                 2281 
                 tgctgctgcc tgggtatctg ggaccccaga tcaggctctg gagacgctgc tacctgaggg 
               
               
                   
               
               
                 2341 
                 gaaggcctga ggtccaggta agaagggaaa atagactggg agtgggacaa gggacttgac 
               
               
                   
               
               
                 2401 
                 tctgctgaac cagatgaaca ggagctggaa aggcaaggag ctgaagcctc tgggagtctg 
               
               
                   
               
               
                 2461 
                 ggaagtgaag ttctactcct cttggcatca aacaaggttt gggagtgtag gaggtgcggg 
               
               
                   
               
               
                 2521 
                 aaagtgcttg tggcttagat taagtggaat ttagggcata gctgaaaggg gaaacagaat 
               
               
                   
               
               
                 2581 
                 taaagacacc agaagtagca gagaagcagg gggccagagc tacaacagta ttcttctctg 
               
               
                   
               
               
                 2641 
                 ttcctctttg cctcctcccc agatgggcct ctcatctccc acaatctctg gcctccagga 
               
               
                   
               
               
                 2701 
                 tgagctatcc catcttcagg agttattacg gaaaggacac caagaatatc tcctgaggat 
               
               
                   
               
               
                 2761 
                 cactccaaga aaagagatcc acataccatt ctcaatccca ctgaaattgc tggcattctc 
               
               
                   
               
               
                 2821 
                 aaaggcaggg cagaggggga tctggggtag agggagggtt ctgtctaatc tttttttttt 
               
               
                   
               
               
                 2881 
                 cttttgtatc tgcacttgca gcctcagctt tcatacttca gcccttaagt tcactaagaa 
               
               
                   
               
               
                 2941 
                 ggtctgagtt tctgctgcag atagtggtgt taactgctcc aactcttgtc ttgcttagtt 
               
               
                   
               
               
                 3001 
                 tctacaaata tttttgcttc ttgtcatttg aaggattaag aaacaaaaac aatccagaaa 
               
               
                   
               
               
                 3061 
                 ttgatcggtt tttttaggcc aatcccatcc cttctggata accagatgtt aaatcatgag 
               
               
                   
               
               
                 3121 
                 atcagagatg ctgttcatca gtcccaacaa gatggcctag aaatcgcatt ctcacctcgc 
               
               
                   
               
               
                 3181 
                 cttgctgctg ctttaattcc aagttctatt tcttccctta tagttttcta tgggaatgag 
               
               
                   
               
               
                 3241 
                 gcggatacag gaaacaccct atctcctctg tatttttgta gtggaatttc tatttaaggg 
               
               
                   
               
               
                 3301 
                 gctcattaaa gcatagtatt tatacac 
               
               
                   
               
             
          
           
               
                 29: BC035625   EGR2 
                   
               
             
          
           
               
                 1 
                 gagcaattga ttaatagctc ggcgagggga ctcactgact gttataataa cactacacca 
                   
               
               
                   
               
               
                 61 
                 gcaactcctg gcttcccagc agccggaaca cagacaggag agagtcagtg gcaaatagac 
               
               
                   
               
               
                 121 
                 atttttctta tttcttaaaa aacagcaact tgtttgctac ttttatttct gttgattttt 
               
               
                   
               
               
                 181 
                 ttttcttggt gtgtgtggtg gttgttttta agtgtggagg gcaaaaggag ataccatccc 
               
               
                   
               
               
                 241 
                 aggctcagtc caacccctct ccaaaacggc ttttctgaca ctccaggtag cgagggagtt 
               
               
                   
               
               
                 301 
                 gggtctccag gttgtgcgag gagcaaatga tgaccgccaa ggccgtagac aaaatcccag 
               
               
                   
               
               
                 361 
                 taactctcag tggttttgtg caccagctgt ctgacaacat ctacccggtg gaggacctcg 
               
               
                   
               
               
                 421 
                 ccgccacgtc ggtgaccatc tttcccaatg ccgaactggg aggccccttt gaccagatga 
               
               
                   
               
               
                 481 
                 acggagtggc cggagatggc atgatcaaca ttgacatgac tggagagaag aggtcgttgg 
               
               
                   
               
               
                 541 
                 atctcccata tcccagcagc tttgctcccg tctctgcacc tagaaaccag accttcactt 
               
               
                   
               
               
                 601 
                 acatgggcaa gttctccatt gaccctcagt accctggtgc cagctgctac ccagaaggca 
               
               
                   
               
               
                 661 
                 taatcaatat tgtgagtgca ggcatcttgc aaggggtcac ttccccagct tcaaccacag 
               
               
                   
               
               
                 721 
                 cctcatccag cgtcacctct gcctccccca acccactggc cacaggaccc ctgggtgtgt 
               
               
                   
               
               
                 781 
                 gcaccatgtc ccagacccag cctgacctgg accacctgta ctctccgcca ccgcctcctc 
               
               
                   
               
               
                 841 
                 ctccttattc tggctgtgca ggagacctct accaggaccc ttctgcgttc ctgtcagcag 
               
               
                   
               
               
                 901 
                 ccaccacctc cacctcttcc tctctggcct acccaccacc tccttcctat ccatccccca 
               
               
                   
               
               
                 961 
                 agccagccac ggacccaggt ctcttcccaa tgatcccaga ctatcctgga ttctttccat 
               
               
                   
               
               
                 1021 
                 ctcagtgcca gagagaccta catggtacag ctggcccaga ccgtaagccc tttccctgcc 
               
               
                   
               
               
                 1081 
                 cactggacac cctgcgggtg ccccctccac tcactccact ctctacaatc cgtaagccct 
               
               
                   
               
               
                 1141 
                 ttccctgccc actggacacc ctgcgggtgc cccctccact cactccactc tctacaatcc 
               
               
                   
               
               
                 1201 
                 gtaactttac cctggggggc cccagtgctg gggtgaccgg accaggggcc agtggaggca 
               
               
                   
               
               
                 1261 
                 gcgagggacc ccggctgcct ggtagcagct cagcagcagc agcagccgcc gccgccgccg 
               
               
                   
               
               
                 1321 
                 cctataaccc acaccacctg ccactgcggc ccattctgag gcctcgcaag taccccaaca 
               
               
                   
               
               
                 1381 
                 gacccagcaa gacgccggtg cacgagaggc cctacccgtg cccagcagaa ggctgcgacc 
               
               
                   
               
               
                 1441 
                 ggcggttctc ccgctctgac gagctgacac ggcacatccg aatccacact gggcataagc 
               
               
                   
               
               
                 1501 
                 ccttccagtg tcggatctgc atgcgcaact tcagccgcag tgaccacctc accacccata 
               
               
                   
               
               
                 1561 
                 tccgcaccca caccggtgag aagcccttcg cctgtgacta ctgtggccga aagtttgccc 
               
               
                   
               
               
                 1621 
                 ggagtgatga gaggaagcgc cacaccaaga tccacctgag acagaaagag cggaaaagca 
               
               
                   
               
               
                 1681 
                 gtgccccctc tgcatcggtg ccagccccct ctacagcctc ctgctctggg ggcgtgcagc 
               
               
                   
               
               
                 1741 
                 ctgggggtac cctgtgcagc agtaacagca gcagtcttgg cggagggccg ctcgcccctt 
               
               
                   
               
               
                 1801 
                 gctcctctcg gacccggaca ccttgagatg agactcaggc tgatacacca gctcccaaag 
               
               
                   
               
               
                 1861 
                 gtcccggagg ccctttgtcc actggagctg cacaacaaac actaccaccc tttcctgtcc 
               
               
                   
               
               
                 1921 
                 ctctctccct ttgttgggca aagggctttg gtggagctag cactgccccc tttccaccta 
               
               
                   
               
               
                 1981 
                 gaagcaggtt cttcctaaaa cttagcccat tctagtctct cttaggtgag ttgactatca 
               
               
                   
               
               
                 2041 
                 acccaaggca aaggggaggc tcagaaggag gtggtgtggg gacccctggc caagagggct 
               
               
                   
               
               
                 2101 
                 gaggtctgac cctgctttaa agggttgttt gactaggttt tgctacccca cttcccctta 
               
               
                   
               
               
                 2161 
                 ttttgaccca tcacaggttt ttgaccctgg atgtcagagt tgatctaaga cgttttctac 
               
               
                   
               
               
                 2221 
                 aataggttgg gagatgctga tcccttcaag tggggacagc aaaaagacaa gcaaaactga 
               
               
                   
               
               
                 2281 
                 tgtgcacttt atggcttggg actgatttgg gggacattgt acagtgagtg aagtatagcc 
               
               
                   
               
               
                 2341 
                 tttatgccac actctgtggc cctaaaatgg tgaatcagag catatctagt tgtctcaacc 
               
               
                   
               
               
                 2401 
                 cttgaagcaa tatgtattat aaactcagag aacagaagtg caatgtgatg ggaggaacat 
               
               
                   
               
               
                 2461 
                 agcaatatct gctccttttc gagttgtttg agaaatgtag gctatttttt cagtgtatat 
               
               
                   
               
               
                 2521 
                 ccactcagat tttgtgtatt tttgatgtac actgttctct aaattctgaa tctttgggaa 
               
               
                   
               
               
                 2581 
                 aaaatgtaaa gcatttatga tctcagaggt taacttattt aagggggatg tacatatatt 
               
               
                   
               
               
                 2641 
                 ctctgaaact aggatgcatg caattgtgtt ggaagtgtcc ttggtgcctt gtgtgatgta 
               
               
                   
               
               
                 2701 
                 gacaatgtta caaggtctgc atgtaaatgg gttgccttat tatggagaaa aaaatcactc 
               
               
                   
               
               
                 2761 
                 cctgagttta gtatggctgt atatttctgc ctattaatat ttggaatttt ttttagaaag 
               
               
                   
               
               
                 2821 
                 tatatttttg tatgctttgt tttgtgactt aaaagtgtta cctttgtagt caaatttcag 
               
               
                   
               
               
                 2881 
                 ataagaatgt acataatgtt accggagctg atttgtttgg tcattagctc ttaatagttg 
               
               
                   
               
               
                 2941 
                 tgaaaaaata aatctattct aacgcaaaac cactaactga agttcagata atggatggtt 
               
               
                   
               
               
                 3001 
                 tgtgactata gtgtaaataa atacttttca acaataaaaa aaaaaaaaaa aaaaaaaaaa 
               
               
                   
               
               
                 3061 
                 a 
               
               
                   
               
             
          
           
               
                 30: X52426   KRT13 
                   
               
             
          
           
               
                 1 
                 ggccaagcaa gcttctatct gcacctgctc tcaatcctgc tctcaccatg agcctccgcc 
                   
               
               
                   
               
               
                 61 
                 tgcagagctc ctctgccagc tatggaggtg gtttcggggg tggctcttgc cagctgggag 
               
               
                   
               
               
                 121 
                 gaggccgtgg tgtctctacc tgttcaactc ggtttgtgtc tgggggatca gctgggggct 
               
               
                   
               
               
                 181 
                 atggaggcgg cgtgagctgt ggttttggtg gaggggctgg tagtggcttt ggaggtggct 
               
               
                   
               
               
                 241 
                 atggaggtgg ccttggaggt ggctatggag gtggccttgg aggtggcttt ggtgggggtt 
               
               
                   
               
               
                 301 
                 ttgctggtgg ctttgttgac tttggtgctt gtgatggcgg cctcctcact ggcaatgaga 
               
               
                   
               
               
                 361 
                 agatcaccat gcagaacctc aacgaccgcc tggcttccta cctggagaag gtgcgcgccc 
               
               
                   
               
               
                 421 
                 tggaggaggc caacgctgac ctggaggtga agatccgtga ctggcacctg aagcagagcc 
               
               
                   
               
               
                 481 
                 cagctagccc tgagcgggac tacagcccct actacaagac cattgaagag ctccgggaca 
               
               
                   
               
               
                 541 
                 agatcctgac cgccaccatt gaaaacaacc gggtcatcct ggagattgac aatgccaggc 
               
               
                   
               
               
                 601 
                 tggctgtgga cgacttcagg ctcaagtatg agaatgagct ggccctgcgc cagagcgtgg 
               
               
                   
               
               
                 661 
                 aggccgacat caacggcctg cgccgggtgc tggatgagct cactctgtct aagactgacc 
               
               
                   
               
               
                 721 
                 tggagatgca gatcgagagc ctgaatgaag agctagccta catgaagaag aaccatgaag 
               
               
                   
               
               
                 781 
                 aggagatgaa ggaatttagc aaccaggtgg tcggccaggt caacgtggag atggatgcca 
               
               
                   
               
               
                 841 
                 ccccaggcat tgacctgacc cgcgtgctgg cagagatgag ggagcagtac gaggccatgg 
               
               
                   
               
               
                 901 
                 cagagaggaa ccgccgggat gctgaggaat ggttccacgc caagagtgca gagctgaaca 
               
               
                   
               
               
                 961 
                 aggaggtgtc taccaacact gccatgattc agaccagcaa gacagagatc acggagctca 
               
               
                   
               
               
                 1021 
                 ggcgcacgct ccaaggcctg gagattgagc tgcagtccca gctgagcatg aaagcggggc 
               
               
                   
               
               
                 1081 
                 tggagaacac ggtggcagag acggagtgcc gctatgccct gcagctgcag cagatccagg 
               
               
                   
               
               
                 1141 
                 gactcatcag cagcatcgag gcccagctga gcgagctccg cagtgagatg gagtgccaga 
               
               
                   
               
               
                 1201 
                 accaagagta caagatgctg ctggacatca agacacgtct ggagcaggag atcgccacct 
               
               
                   
               
               
                 1261 
                 accgcagcct gctcgagggc caggacgcca agaagcgtca gcccccgtag cacctctgtt 
               
               
                   
               
               
                 1321 
                 accacgactt ctagtgcctc tgttaccacc acctctaatg cctctggtcg ccgcacttct 
               
               
                   
               
               
                 1381 
                 gatgtccgta ggccttaaat ctgcctggcg tcccctccct ctgtcttcag cacccagagg 
               
               
                   
               
               
                 1441 
                 aggagagagc cggcagttcc ctgcaggaga gaggaggggc tgctggaccc aaggctcagt 
               
               
                   
               
               
                 1501 
                 ccctctgctc tcaggacccc ctgtcctgac tctctcctga tggtgggccc tctgtgctct 
               
               
                   
               
               
                 1561 
                 tctcttccgg tcggatctct ctcctctctg acctggatac gctttggttt ctcaacttct 
               
               
                   
               
               
                 1621 
                 ctaccccaaa gaaaagatta ttcaataaag tttcctgcct ttctgcaaac ataaaaa 
               
               
                   
               
             
          
           
               
                 31: NM_005504   BCAT1 
                   
               
             
          
           
               
                 1 
                 tttgcttgca acactggcac ctctgccctg caccccggga gtgagcagtg agtgaggctc 
                   
               
               
                   
               
               
                 61 
                 gggtctgggc gctggctccg aatcttcggg ctgggagaga ctccaccatc tgggggcggc 
               
               
                   
               
               
                 121 
                 ctgggggagc agccttagtg tcttcctgct gatgcaatcc gctaggtcgc gagtctccgc 
               
               
                   
               
               
                 181 
                 cgcgagaggg ccggtctgca atccagcccg ccacgtgtac tcgccgccgc ctcgggcact 
               
               
                   
               
               
                 241 
                 gccccaggtc ttgctgcagc cgggaccgcg ctctgcagcc gcagacccgg tccacacggc 
               
               
                   
               
               
                 301 
                 caggggctac gacccttggg atctgccctc cgctcagctc gagcttccct cgtggccgac 
               
               
                   
               
               
                 361 
                 ggaacaatga aggattgcag taacggatgc tccgcagagt gtaccggaga aggaggatca 
               
               
                   
               
               
                 421 
                 aaagaggtgg tggggacttt taaggctaaa gacctaatag tcacaccagc taccatttta 
               
               
                   
               
               
                 481 
                 aaggaaaaac cagaccccaa taatctggtt tttggaactg tgttcacgga tcatatgctg 
               
               
                   
               
               
                 541 
                 acggtggagt ggtcctcaga gtttggatgg gagaaacctc atatcaagcc tcttcagaac 
               
               
                   
               
               
                 601 
                 ctgtcattgc accctggctc atcagctttg cactatgcag tggaattatt tgaaggattg 
               
               
                   
               
               
                 661 
                 aaggcatttc gaggagtaga taataaaatt cgactgtttc agccaaacct caacatggat 
               
               
                   
               
               
                 721 
                 agaatgtatc gctctgctgt gagggcaact ctgccggtat ttgacaaaga agagctctta 
               
               
                   
               
               
                 781 
                 gagtgtattc aacagcttgt gaaattggat caagaatggg tcccatattc aacatctgct 
               
               
                   
               
               
                 841 
                 agtctgtata ttcgtcctac attcattgga actgagcctt ctcttggagt caagaagcct 
               
               
                   
               
               
                 901 
                 accaaagccc tgctctttgt actcttgagc ccagtgggac cttatttttc aagtggaacc 
               
               
                   
               
               
                 961 
                 tttaatccag tgtccctgtg ggccaatccc aagtatgtaa gagcctggaa aggtggaact 
               
               
                   
               
               
                 1021 
                 ggggactgca agatgggagg gaattacggc tcatctcttt ttgcccaatg tgaagcagta 
               
               
                   
               
               
                 1081 
                 gataatgggt gtcagcaggt cctgtggctc tatggagagg accatcagat cactgaagtg 
               
               
                   
               
               
                 1141 
                 ggaactatga atctttttct ttactggata aatgaagatg gagaagaaga actggcaact 
               
               
                   
               
               
                 1201 
                 cctccactag atggcatcat tcttccagga gtgacaaggc ggtgcattct ggacctggca 
               
               
                   
               
               
                 1261 
                 catcagtggg gtgaatttaa ggtgtcagag agatacctca ccatggatga cttgacaaca 
               
               
                   
               
               
                 1321 
                 gccctggagg ggaacagagt gagagagatg tttggctctg gtacagcctg tgttgtttgc 
               
               
                   
               
               
                 1381 
                 ccagtttctg atatactgta caaaggcgag acaatacaca ttccaactat ggagaatggt 
               
               
                   
               
               
                 1441 
                 cctaagctgg caagccgcat cttgagcaaa ttaactgata tccagtatgg aagagaagag 
               
               
                   
               
               
                 1501 
                 agcgactgga caattgtgct atcctgaatg gaaaatagag gatacaatgg aaaatagagg 
               
               
                   
               
               
                 1561 
                 ataccaactg tatgctactg ggacagactg ttgcatttga attgtgatag atttctttgg 
               
               
                   
               
               
                 1621 
                 ctacctgtgc ataatgtagt ttgtagtatc aatgtgttac aagagtgatt gtttcttcat 
               
               
                   
               
               
                 1681 
                 gccagagaaa atgaattgca atcatcaaat ggtgtttcat aacttggtag tagtaactta 
               
               
                   
               
               
                 1741 
                 ccttacctta cctagaaaaa cattaatgta agccatataa catgggattt tcctcaatga 
               
               
                   
               
               
                 1801 
                 ttttagtgcc tccttttgta cttcactcag atactaaata gtagtttatt ctttaatata 
               
               
                   
               
               
                 1861 
                 agttacattc tgctcctcaa acaaatgcaa ttttttgtgt gtgtttgaaa gctaatttga 
               
               
                   
               
               
                 1921 
                 gaaaatttca taggttacat ttcctgcagc ctatctttat ccacagaaag tgttttcttt 
               
               
                   
               
               
                 1981 
                 tttttaaatc aagactttta aaactggatt tcctcccatc actgtttttt gaaggtcctc 
               
               
                   
               
               
                 2041 
                 caagtccgtg ttaaggtaaa tatctgtttt cttcctgatg tcacagcctg agcatactct 
               
               
                   
               
               
                 2101 
                 gtgcattagg aagacctgag tgcatttccc accattgtcc tttccacatt atgttgtagc 
               
               
                   
               
               
                 2161 
                 tggctggctg tcaggcgact acaagactga gggtcttgtg ccttatagat ctttgtatcc 
               
               
                   
               
               
                 2221 
                 cccatggctg acatatagta ggtactcagt aaatggtttt ataatgaatc agtgaacatt 
               
               
                   
               
               
                 2281 
                 ttgcttctat agaagtgtac cttctttgtt tctatattat gaaacctctt tattagaatt 
               
               
                   
               
               
                 2341 
                 tgtgattgat tctgacagtg tatagattta ccttatattg tctttatttt ccatgagcta 
               
               
                   
               
               
                 2401 
                 ctaagtcatt agagatactc tgaagcatag ttagtttagg aaatcacttc atattgattg 
               
               
                   
               
               
                 2461 
                 tattagaatt atcttggaat tgaagatata tccctagagc aggggacccc aacccccagg 
               
               
                   
               
               
                 2521 
                 ccatgggcca cacagcagga agaggtgagt ggtgggccat tgaggagctt catctgtatt 
               
               
                   
               
               
                 2581 
                 tatggctact tcccatcact cgaattacca cctgaactcc acctcttgtc agctcagtgg 
               
               
                   
               
               
                 2641 
                 cagcattaga ttctcatagg agcacaaatc ctattgtgaa ctctgcatgc aagggatcta 
               
               
                   
               
               
                 2701 
                 ggctatgcgc tccttatgag aatctaatgc ttgatgacct gaggtgtaac agtttcatcc 
               
               
                   
               
               
                 2761 
                 tgaaaccacc cttcaccctg cagtctgtgg aaaaattgtc ttccacaaaa ctggtccctg 
               
               
                   
               
               
                 2821 
                 gtgccaaaaa tgttggggac cactgctcta gagagaggtc atgatatcat accaaccaaa 
               
               
                   
               
               
                 2881 
                 tggaaatgac aaatgtttta tgtcaagtgt taattgcaga aataaatctt tttttttttt 
               
               
                   
               
               
                 2941 
                 ttttggtaga aaacaaagag gcatactctg atttttatac tctgtttttg caggtgctct 
               
               
                   
               
               
                 3001 
                 tttctttgaa tggagatttg atgagcaagt ggttaggatg cagggagagc tactatgggt 
               
               
                   
               
               
                 3061 
                 gatattttcc ttgtttagga gctgtgagtt aaaattgtat cctttgtggt ttatctaagg 
               
               
                   
               
               
                 3121 
                 aaagtcaaat cttgacagaa aacatttttc cttggaaggt caactctcag acattgtatt 
               
               
                   
               
               
                 3181 
                 ttggtttccc tcagtcctca taacttcctt cttgctgaac atattttatt ctcttttcag 
               
               
                   
               
               
                 3241 
                 agaaggaaaa taaaaaggat tctaaaagtt tgatgcattg gaaaaatttc cttgaggcat 
               
               
                   
               
               
                 3301 
                 ttagcaacac atagaaaatg ggctttgatt cttttccaaa acttttagcc atagggtctt 
               
               
                   
               
               
                 3361 
                 ttatagacag ggatagtaaa atgaaaattg agaaatataa gatgaaaagg aatgataaaa 
               
               
                   
               
               
                 3421 
                 atatctttta gggggctttt aattggtgat ctgaaatctt gggagaagct gttcttttca 
               
               
                   
               
               
                 3481 
                 ggcctgaggt gctcttgact gtcgcctgcg cactgtgtac cccgagcaac attctaaggg 
               
               
                   
               
               
                 3541 
                 tgtgctttcg ccttggctaa ctcctttgac ctcattcttc atatagtagt ctaggaaaaa 
               
               
                   
               
               
                 3601 
                 gttgcaggta atttaaactg tctagtggta catagtaact aaatttctat tcctatgaga 
               
               
                   
               
               
                 3661 
                 aatgagaatt atttatttgc catcaacaca ttttatactt tgcatctcca aatttattgt 
               
               
                   
               
               
                 3721 
                 ggcgagactt gtccattgtg aaagttagag aacattatgt ttgtatcatt tctttcataa 
               
               
                   
               
               
                 3781 
                 aacctcaaga gcatttttaa gcccttttca tcagacccag tgaaaactaa ggatagatgt 
               
               
                   
               
               
                 3841 
                 ttaaaaactg gaggtctcct gataaggaga acacaatcca ccattgtcat ttaagtaata 
               
               
                   
               
               
                 3901 
                 agacaggaaa ttgaccttga cgctttcttg ttaaatagat ttaacaggaa catctgcaca 
               
               
                   
               
               
                 3961 
                 tcttttttcc ttgtgcacta tttgtttaat tgcagtggat taatacagca agagtgccac 
               
               
                   
               
               
                 4021 
                 attataacta ggcaattatc cattcttcaa gacttagtta ttgtcacact aattgatcgt 
               
               
                   
               
               
                 4081 
                 ttaaggcata agatggtcta gcattaggaa catgtgaagc taatctgctc aaaaagatca 
               
               
                   
               
               
                 4141 
                 acaaattaat attgttgctg atatttgcat aattggctgc aattatttaa tgtttaattg 
               
               
                   
               
               
                 4201 
                 ggttgatcaa atgagattca gcaattcaca agtgcattaa tataaacaga actggtggca 
               
               
                   
               
               
                 4261 
                 cttaaaatga taatgattaa cttatattgc atgttctctt cctttcactt ttttcagttt 
               
               
                   
               
               
                 4321 
                 ctacatttca gaccgagctt gtcagctttt ttgaaaacac atcagtagaa accaagattt 
               
               
                   
               
               
                 4381 
                 taaaatgaag tgtcaagaca aaggcaaaac ctgagcagtt cctaaaaaga tttgctgtta 
               
               
                   
               
               
                 4441 
                 gaaattttct ttgtggcagt catttattaa ggattcaact cgtgatacac caaaagaaga 
               
               
                   
               
               
                 4501 
                 gttgacttca gagatgtgtt ccatgctctc tagcacagga atgaataaat ttataacacc 
               
               
                   
               
               
                 4561 
                 tgctttagcc tttgttttca aaagcacaaa ggaaaagtga aagggaaaga gaaacaagtg 
               
               
                   
               
               
                 4621 
                 actgagaagt cttgttaagg aatcaggttt tttctacctg gtaaacattc tctattcttt 
               
               
                   
               
               
                 4681 
                 tctcaaaaga ttgctgtaag aaaaaatgta agacaaaaaa aaaaaaaaaa aacaaacaga 
               
               
                   
               
               
                 4741 
                 ggcagaggca ggcagtagca agaaagcaga gcgtaacatc agctagatgg taacatgcaa 
               
               
                   
               
               
                 4801 
                 tgtcagctct cttgaagaca tgggaaacct aagttacacc ttgggttaaa attcttcacc 
               
               
                   
               
               
                 4861 
                 atattagttt tgttgcttca taaaatttac ctaagcaagt ggtcttgctt gcctcaaatc 
               
               
                   
               
               
                 4921 
                 caagcagtct tgaacacttg gaggcaatta atgagtatat cttagtcaaa agaattgttg 
               
               
                   
               
               
                 4981 
                 gagcttttta ttaaagctac agtttcagtt ctgcttttgg ggaattgtgc tatgaaagca 
               
               
                   
               
               
                 5041 
                 gctgccaaaa taagctcatt tattttcttc aatcccactc agtgctcagt cactatattc 
               
               
                   
               
               
                 5101 
                 tgtttccttt ttttttttca agttgcatat ttggtttccc cttatgattg ggaaagatga 
               
               
                   
               
               
                 5161 
                 attttcagca gaaaacattg tttgttcact ttcaaagagt gatagtttct aaaacattta 
               
               
                   
               
               
                 5221 
                 gagcaataaa tattcatcag aggtaccaag taagccggca gaagagttaa gggttagaga 
               
               
                   
               
               
                 5281 
                 aatcccttat ttcatgtctt gactctaaaa ttatcaaagt acttttcctt gtaatgtgga 
               
               
                   
               
               
                 5341 
                 tttcttctta tgcggatatg caaaaacttc agttatacgt agtaatgcta gcaggtaatt 
               
               
                   
               
               
                 5401 
                 ttagtagaca ttttataaca actgtcactt tgtttcgcca catgtagagt ttgttcagct 
               
               
                   
               
               
                 5461 
                 attttccaga tatctcccca caaaaggagg caaagggtac cagcttttca atgagcatta 
               
               
                   
               
               
                 5521 
                 cctattactt ggcaaagatg atgaagactc tattaatagt tcatttgata aatgttgaca 
               
               
                   
               
               
                 5581 
                 taaccaacaa tagagattag gaagttagtt ttaagaaatc aatggcatat agacattacc 
               
               
                   
               
               
                 5641 
                 ctcatggagt ttgtattcta ctacttgaac tgattgtagc tataaaagca tagttagata 
               
               
                   
               
               
                 5701 
                 gctgaatagt tagatcataa gcaaagaagg ccagaacaca tctcttatca agaaatcaat 
               
               
                   
               
               
                 5761 
                 gaatagttta tctcattttt aaagcaactt tatccttctt taattccttc ctttcttcta 
               
               
                   
               
               
                 5821 
                 gtgcaaaact acttaataag gttggtgttt aggttagtgt tcacaccatt cctcatctgg 
               
               
                   
               
               
                 5881 
                 tgtgaattac cttctctttc tttactattt actaccaacc tagtacatgt gttgactgaa 
               
               
                   
               
               
                 5941 
                 ttcttttcaa acaatgttga gttatcatgg tgcacctaat aaattaacac cacagattac 
               
               
                   
               
               
                 6001 
                 agcatccttg ctgattttct cagcaaagcc agattagatg gaaataaaca aagaaaatga 
               
               
                   
               
               
                 6061 
                 tcctagagtg aatttttcta gaaaatatct attatgaacc atgctgttta aagtattagc 
               
               
                   
               
               
                 6121 
                 ttgaaggtga tggatccagc tattcagaaa ataactttca tataaccatg attttgcaca 
               
               
                   
               
               
                 6181 
                 gtatgaggtc ttaaatgtgt ggaaagagat aaatttttta tcattaccac aaaccccttt 
               
               
                   
               
               
                 6241 
                 taaagattca aaggtggaag aaagtgattt attttttctc ttcagcatac atatataaaa 
               
               
                   
               
               
                 6301 
                 gacttgtcag atgtttaatt tggggaggtt gataatgaaa catatcaaca gagtatagta 
               
               
                   
               
               
                 6361 
                 gttatagtag tgtttgtggg taaataattt cctggggtca gacatatata aacatatttg 
               
               
                   
               
               
                 6421 
                 cttcaaaatg ataaaggcat gaaatcagtc ttaaaaattg aaatgggggt gatgggggag 
               
               
                   
               
               
                 6481 
                 aaaaagaaga acaaatttga agtgcccttt caaatctgct ggatacaagt attgaagttt 
               
               
                   
               
               
                 6541 
                 taagtcatct tattctgtct gaaagtgtat ttttcattct acaatagacc caatcaacaa 
               
               
                   
               
               
                 6601 
                 gacgtataac ttgagttgca tgatgttcag tttatgtaat ctactgttgg gatggtaaga 
               
               
                   
               
               
                 6661 
                 attgatgtag gctgtggtgt aagaatgaat taaaatatag tttcactggc ttttctctac 
               
               
                   
               
               
                 6721 
                 atatccacta tcacaatggc taggtttcct gttgctcact attggattct ggagaaaaat 
               
               
                   
               
               
                 6781 
                 ttaatgaaag atgatatcag aggaagaata agtggaggta gagaagaaag gaatgataga 
               
               
                   
               
               
                 6841 
                 ggaggggaaa aaaacaaaac atatttttgt gttatccaaa ggagcttttt ccttattctg 
               
               
                   
               
               
                 6901 
                 tcaagcattg agatcttctt cagctttcaa tgtagttgct aaatacaaat aatgctacta 
               
               
                   
               
               
                 6961 
                 ggtagtgact aaatatagca aacacttcat cagatattag aattaggtca cactattgag 
               
               
                   
               
               
                 7021 
                 gttataatct gaaggttgtg ttacatagaa accactttag attattatca acttggacta 
               
               
                   
               
               
                 7081 
                 ggctttattt tataatagca tagtaagtaa tatctattgt gtcatttctt caaccatttt 
               
               
                   
               
               
                 7141 
                 attctaagat ccatgaagct tcttgaggcc aaataaaata ataagtttag acaagaagta 
               
               
                   
               
               
                 7201 
                 gattgtgact tttttccctt agagatacta tttactatct cctatcctga taggtggaag 
               
               
                   
               
               
                 7261 
                 gtttactgaa ttggaaattg gttgactatt agtttttaac taaaatgtgc aataacacat 
               
               
                   
               
               
                 7321 
                 tgcagtttcc tcaaactagt ttcctatgat cattaaactc attctcaggg ttaagaaagg 
               
               
                   
               
               
                 7381 
                 aatgtaaatt tctgcctcaa tttgtacttc atcaataagt ttttgaagag tgcagatttt 
               
               
                   
               
               
                 7441 
                 tagtcaggtc ttaaaaataa actcacaaat ctggatgcat ttctaaattc tgcaaatgtt 
               
               
                   
               
               
                 7501 
                 tcctggggtg acttaacaag gaataatccc acaatatacc tagctaccta atacatggag 
               
               
                   
               
               
                 7561 
                 ctggggctca acccactgtt tttaaggatt tgcgcttact tgtggctgag gaaaaataag 
               
               
                   
               
               
                 7621 
                 tagttcgagg aagtagtttt taaatgtgag cttatagata gaaacagaat atcaacttaa 
               
               
                   
               
               
                 7681 
                 ttatgaaatt gttagaacct gttctcttgt atctgaatct gattgcaatt actattgtac 
               
               
                   
               
               
                 7741 
                 tgatagactc cagccattgc aagtctcaga tatcttagct gtgtagtgat tcttgaaatt 
               
               
                   
               
               
                 7801 
                 ctttttaaga aaaattgagt agaaagaaat aaaccctttg taaatgaggc ttggcttttg 
               
               
                   
               
               
                 7861 
                 tgaaagatca tccgcaggct atgttaaaag gattttagct cactaaaagt gtaataatgg 
               
               
                   
               
               
                 7921 
                 aaatgtggaa aatatcgtag gtaaaggaaa ctacctcatg ctctgaaggt tttgtagaag 
               
               
                   
               
               
                 7981 
                 cacaattaaa catctaaaat ggctttgtta caccagagcc atctggtgtg aagaactcta 
               
               
                   
               
               
                 8041 
                 tatttgtatg ttgagagggc atggaataat tgtattttgc tggcaataga cacattcttt 
               
               
                   
               
               
                 8101 
                 attatttgca gattcctcat caaatctgta attatgcaca gtttctgtta tcaataaaac 
               
               
                   
               
               
                 8161 
                 aaaagaatcc tgtttgtgtg gtttcatgaa a 
               
               
                   
               
             
          
           
               
                 32: NM_006643   SDCCAG3 
                   
               
             
          
           
               
                 1 
                 cacgggcgga gccggggcca tggagccgcc gctgccgggc taggcaggtc gtgccccgcc 
                   
               
               
                   
               
               
                 61 
                 gggccggcgg cgatgtcggg ctaccagcgc cacccgggcg ccaccccgct gtcccgagcc 
               
               
                   
               
               
                 121 
                 cggagcctcg ccattcccga cgctccagcg ttctatgagc gccggtcttg tctcccccag 
               
               
                   
               
               
                 181 
                 ctaaattgtg agcgccccca tggcagggac ctggactccc ccttcttcgg cattcggccg 
               
               
                   
               
               
                 241 
                 gcctttatgt gctatgtgcc cagcccggtg ctggcttccg tgggagacac agatgacaga 
               
               
                   
               
               
                 301 
                 tttgaagatc tggaagaggc aaatccattc tcttttagag agtttctgaa gaccaagaac 
               
               
                   
               
               
                 361 
                 ctcggcctct cgaaagagga tccggccagc agaatttatg caaaggaagc ctcgaggcat 
               
               
                   
               
               
                 421 
                 tccctgggac ttgaccacaa ctccccaccc tcccaaaccg gcgggtatgg cctggagtat 
               
               
                   
               
               
                 481 
                 cagcagccat ttttcgagga tccgacaggg gctggtgacc tcctggatgg ggaggaggat 
               
               
                   
               
               
                 541 
                 gaggacaccg gatggagtgg ggcctacctg ccgtccgcca tcgagcagac tcaccccgag 
               
               
                   
               
               
                 601 
                 agggtccctg ccggcacgtc gccctgcagc acataccttt cctttttctc caccccgtcg 
               
               
                   
               
               
                 661 
                 gagctggcag ggcctgagtc tctgccctcg tgggcgttga gtgacactga ttctcgcgtg 
               
               
                   
               
               
                 721 
                 tctccggcct ctccggcagg gagtcctagc gcagactttg cggttcatgg agagtctctg 
               
               
                   
               
               
                 781 
                 ggagacaggc acctgcggac gctgcagata agttacgacg cactgaaaga tgaaaattct 
               
               
                   
               
               
                 841 
                 aagctgagaa gaaagctgaa tgaggttcag agcttctctg aagctcaaac agaaatggtg 
               
               
                   
               
               
                 901 
                 aggacgcttg agcggaagtt agaagcaaaa atgatcaagg aggaaagcga ctaccacgac 
               
               
                   
               
               
                 961 
                 ctggagtcgg tggttcagca ggtggagcag aacctggagc tgatgaccaa acgggctgta 
               
               
                   
               
               
                 1021 
                 aaggcagaaa accacgtcgt gaaactaaaa caggaaatca gtttgctcca ggcgcaggtc 
               
               
                   
               
               
                 1081 
                 tccaacttcc agcgagagaa tgaagccctg cggtgcggcc agggcgccag cctgaccgtg 
               
               
                   
               
               
                 1141 
                 gtgaagcaga acgccgacgt ggccctgcag aacctccggg tggtcatgaa cagtgcacag 
               
               
                   
               
               
                 1201 
                 gcttccatca agcaactggt ttccggagct gagacactga atcttgttgc cgaaatcctt 
               
               
                   
               
               
                 1261 
                 aaatctatag acagaatttc tgaaattaaa gacgaggagg aagactcttg aggacccctg 
               
               
                   
               
               
                 1321 
                 ggtgttctca gcatgaagct ccgtgtatac cctgaggtca ccaccgctcg atctaaatgt 
               
               
                   
               
               
                 1381 
                 gcagttgtgt ccttaaatat gcagtcttca cccagagtaa agtgttgatc gcaagagtcc 
               
               
                   
               
               
                 1441 
                 agtgtcgtgc cctcagccag ttcttggcca ccacaatggg agcagccctg gccgagttgt 
               
               
                   
               
               
                 1501 
                 ctctgtggtt tctatgcagc ccttcttggc gaaattcctg cgatcttata gattctaatg 
               
               
                   
               
               
                 1561 
                 agctcttgga agacattgtc ataaaagcca gtgattttaa gaaaaagagt ggttctggaa 
               
               
                   
               
               
                 1621 
                 tcagtgtttt ccagtcccat cccagaacat cagttgtaag ataagtacaa ttggttgtcc 
               
               
                   
               
               
                 1681 
                 ttgatttcat aagtagaaca aacactaaat gtgcctctga gatggccacc ccgggcaggg 
               
               
                   
               
               
                 1741 
                 acctgtgcct tccaccgatg ctcagggctc cctctggctc ccgggtcact cttgtggccc 
               
               
                   
               
               
                 1801 
                 cagtgggtgg tccctgcagt catggcctga gtgcgcaggg gccaccgcgt ggctgccgct 
               
               
                   
               
               
                 1861 
                 gtcctcctcc gggacccacg gggaccaagg tcacacgttc cgtgctgtga agctgtccag 
               
               
                   
               
               
                 1921 
                 atgtgcctct ttggctgggg gttctggtgg acgtttcaag tggcattttg tacaatgcag 
               
               
                   
               
               
                 1981 
                 gttagaattc aggaatttca agtatgtgcc cgggtctgtc aggtcccagt tgcctttctg 
               
               
                   
               
               
                 2041 
                 acggcccccc tcagagggac ggcgatgagc actaaatgct tttttgacta ttttcctata 
               
               
                   
               
               
                 2101 
                 gatttttttt aaaacttttt tttcctcctg ttccaattga tagctttctt atttaataaa 
               
               
                   
               
               
                 2161 
                 ttctgtagtt caccaaaaaa aaaaaaaaaa a 
               
               
                   
               
             
          
           
               
                 33: AA464095   PIGK 
                   
               
             
          
           
               
                 1 
                 atatattccc agctagttga aaatgatgat tcccacaaga agcataactc agcttgtttc 
                   
               
               
                   
               
               
                 61 
                 tgcttactga gtattttcta ctatggtata tattgataac atttcttcca ttatgtatgt 
               
               
                   
               
               
                 121 
                 tgtataccag agttacagtt actgtgggaa tcataatttg aaattttgac tcctgtgttt 
               
               
                   
               
               
                 181 
                 ctggaatctt tacaacaaat gttgcattaa catataactt ttttcagttg actttaccaa 
               
               
                   
               
               
                 241 
                 aattaagccc atctttagta gatactgttt taacatgtga aagaaatacg ttataaacat 
               
               
                   
               
               
                 301 
                 accacaagat atggctataa aacaatgaga tcagtatcca tttttgcttt aaagaattgg 
               
               
                   
               
               
                 361 
                 ccttattgct tcagtgtcac atctcatact caagggcatt tactacaaay aaagagttct 
               
               
                   
               
               
                 421 
                 ccaatattgc tgttctgttg ctgcctgccc tatttacaca tgt 
               
               
                   
               
             
          
           
               
                 34: AA961188   MRPS9 
                   
               
             
          
           
               
                 1 
                 tttacactta tagtagactt tatttagtga atccaaatga catgtgataa ttgtttggaa 
                   
               
               
                   
               
               
                 61 
                 aggcctattg attttatatc tgatcattca atccagagac attaaattca gttgattaat 
               
               
                   
               
               
                 121 
                 ggagttcccc aactgtaaga cttctttacg agattatttt caagctttga aaagatcttc 
               
               
                   
               
               
                 181 
                 tgagataaag gggatcagca aacagtaaga gtgtgttgct atacccaagc aaaagaaata 
               
               
                   
               
               
                 241 
                 aatcttaatc tctcagcaaa tcattcaaaa tgtcagaaat gttagtgttt ctatatcttg 
               
               
                   
               
               
                 301 
                 gtaaaatgga ttgattgaga agtatgaaaa gtataacagt ggcatgcaga atattgtttt 
               
               
                   
               
               
                 361 
                 tatgaatatt cagaatttca gttgtttaca taa 
               
               
                   
               
             
          
           
               
                 35: NM_0181836   ASPM 
                   
               
             
          
           
               
                 1 
                 atggcgaacc ggcgagtggg gcgaggctgc tgggaagtga gcccgaccga gcggaggccg 
                   
               
               
                   
               
               
                 61 
                 cccgcggggc tgcggggccc cgcggccgag gaggaggcgt cttccccgcc ggtcctgtct 
               
               
                   
               
               
                 121 
                 ctcagccact tctgcaggtc tcctttcctt tgcttcgggg acgttctcct gggagcctca 
               
               
                   
               
               
                 181 
                 cggacgctgt ctctggccct agacaaccct aacgaggagg tggcagaagt gaagatctcc 
               
               
                   
               
               
                 241 
                 cacttcccgg ccgcggacct gggcttcagt gtgtcgcagc gctgtttcgt gttgcagcct 
               
               
                   
               
               
                 301 
                 aaagagaaaa ttgttatttc tgttaactgg acaccactca aagaaggccg agtaagagag 
               
               
                   
               
               
                 361 
                 attatgacat ttcttgtaaa tgatgttctg aaacaccaag ctatattact aggaaatgca 
               
               
                   
               
               
                 421 
                 gaagagcaga aaaagaaaaa gaggagtctt tgggatacca ttaaaaagaa gaaaatttca 
               
               
                   
               
               
                 481 
                 gcctctacaa gtcacaacag aagggtttca aatattcaga atgttaataa aacatttagt 
               
               
                   
               
               
                 541 
                 gtttcccaaa aagttgacag agttaggagc ccactacaag cttgtgaaaa cttggctatg 
               
               
                   
               
               
                 601 
                 aatgaaggcg gtcccccaac agaaaacaat tctttaatac ttgaagaaaa taaaataccc 
               
               
                   
               
               
                 661 
                 atatcaccta ttagccctgc tttcaatgaa tgccatggtg caacttgctt gccactctct 
               
               
                   
               
               
                 721 
                 gtacgtcgat ctactaccta ctcatctctt catgcatcag aaaataggga actattaaat 
               
               
                   
               
               
                 781 
                 gtacacagtg ccaacgtttc aaaagtttct tttaatgaga aagctgtaac tgaaacttcc 
               
               
                   
               
               
                 841 
                 tttaattctg taaatgttaa tggccaaaga ggagagaata gtaaacttag tcttaccccc 
               
               
                   
               
               
                 901 
                 aactgttctt caactttgaa cattacacaa agccaaatac attttctaag tccagattct 
               
               
                   
               
               
                 961 
                 tttgtaaata atagtcatgg agctaataat gaactagaat tagtaacatg tctttcatca 
               
               
                   
               
               
                 1021 
                 gatatgttta tgaaagataa ttcacagcct gtgcatttgg aatcaacaat tgcacatgaa 
               
               
                   
               
               
                 1081 
                 atttatcaga aaattttaag tccagattct ttcataaaag ataattatgg actaaatcag 
               
               
                   
               
               
                 1141 
                 gatctagaat cagagtcagt taatcctatt ttatccccta atcaattttt aaaagataac 
               
               
                   
               
               
                 1201 
                 atggcatata tgtgtacatc tcagcaaaca tgtaaagtac cattatcaaa tgaaaattct 
               
               
                   
               
               
                 1261 
                 caagtcccac agtctcctga agattggaga aaaagtgaag tttcgccacg tattcctgaa 
               
               
                   
               
               
                 1321 
                 tgtcagggtt caaaatctcc caaagctatt tttgaagaac tagtagaaat gaagtcaaat 
               
               
                   
               
               
                 1381 
                 tactacagtt ttataaaaca aaataatcct aaattttctg cagttcagga tatttctagt 
               
               
                   
               
               
                 1441 
                 catagccaca ataaacaacc taagagacgt ccaatacttt ctgccactgt tactaaaagg 
               
               
                   
               
               
                 1501 
                 aaggccacct gtaccagaga aaaccaaact gagattaata aaccaaaagc aaaaagatgt 
               
               
                   
               
               
                 1561 
                 ctcaacagtg cagtgggtga acatgaaaaa gtaataaata atcaaaagga aaaagaagat 
               
               
                   
               
               
                 1621 
                 tttcattctt atcttccaat tatagatcca atattaagta aatctaagag ttataaaaac 
               
               
                   
               
               
                 1681 
                 gaggtaacac cctcttcgac aacagcttca gttgctcgga aaagaaagag cgatggaagc 
               
               
                   
               
               
                 1741 
                 atggaagatg caaatgtgag agttgcaatt acagaacata cagaagtgcg agaaatcaaa 
               
               
                   
               
               
                 1801 
                 agaatccatt tttctccctc agagcctaaa acatcagctg ttaagaaaac aaaaaatgtg 
               
               
                   
               
               
                 1861 
                 acaacaccca tctcaaaacg tattagcaac agagagaaat taaacctgaa gaagaaaact 
               
               
                   
               
               
                 1921 
                 gatttatcaa tattcagaac tccaatttct aaaacaaaca aaaggacaaa acccattatc 
               
               
                   
               
               
                 1981 
                 gctgtggcac agtccagttt gaccttcata aaaccattaa aaacagatat tcccagacac 
               
               
                   
               
               
                 2041 
                 ccgatgccat ttgctgcaaa aaacatgttt tatgatgaac gctggaagga aaagcaggaa 
               
               
                   
               
               
                 2101 
                 cagggcttca cttggtggtt aaattttata ttaacccctg atgacttcac tgtaaaaaca 
               
               
                   
               
               
                 2161 
                 aatatttctg aagtaaatgc tgctactctt cttttgggaa tagagaatca acataaaata 
               
               
                   
               
               
                 2221 
                 agtgttccta gagcacctac aaaagaggaa atgtctctca gagcttatac tgctcggtgt 
               
               
                   
               
               
                 2281 
                 aggttaaaca gactacgtcg tgcagcatgc cgtttgttta cttctgaaaa aatggttaaa 
               
               
                   
               
               
                 2341 
                 gctattaaaa agcttgaaat tgaaattgaa gctaggcggt taattgttcg aaaagataga 
               
               
                   
               
               
                 2401 
                 cacctatgga aagatgtggg agaacgtcag aaagtcctga attggctgtt gtcctacaat 
               
               
                   
               
               
                 2461 
                 cctttgtggc ttcgaattgg tctagagaca acttatggag aactcatatc tttggaagat 
               
               
                   
               
               
                 2521 
                 aacagtgatg tcacagggtt ggctatgttt attctgaatc gcctactttg gaatcctgat 
               
               
                   
               
               
                 2581 
                 atagcagctg agtatagaca ccccactgtt cctcacctgt atagagatgg tcatgaagaa 
               
               
                   
               
               
                 2641 
                 gctttgtcca agtttacatt gaaaaagtta ttgttgttgg tctgttttct tgattatgct 
               
               
                   
               
               
                 2701 
                 aaaatttcca gactcattga tcatgatcct tgtctcttct gtaaagatgc cgaattcaag 
               
               
                   
               
               
                 2761 
                 gctagtaaag aaatcctttt ggctttttca cgagatttcc taagtggtga aggtgacctt 
               
               
                   
               
               
                 2821 
                 tcccgtcacc ttggcttatt gggattacct gttaaccatg ttcagacacc atttgatgaa 
               
               
                   
               
               
                 2881 
                 tttgattttg ccgttacaaa tcttgccgta gacttgcaat gtggagtgcg ccttgtgcga 
               
               
                   
               
               
                 2941 
                 accatggaac ttctcacaca gaactgggac ctctcaaaga aactcaggat tccggcaata 
               
               
                   
               
               
                 3001 
                 agtcgtcttc aaaagatgca caatgttgac attgttcttc aagttcttaa atcacgagga 
               
               
                   
               
               
                 3061 
                 attgaattaa gtgatgagca tggaaataca attctatcta aggatattgt ggataggcac 
               
               
                   
               
               
                 3121 
                 agagaaaaaa ctctcaggtt gctttggaaa atagcgtttg cttttcaggt ggatatttcc 
               
               
                   
               
               
                 3181 
                 cttaacttag atcaattaaa ggaagaaatt gcctttctaa aacacacaaa gagtataaag 
               
               
                   
               
               
                 3241 
                 aaaacaatat ctctactatc atgccattct gatgatctta ttaataagaa aaaaggcaaa 
               
               
                   
               
               
                 3301 
                 agggatagtg gttcctttga acaatatagt gaaaacataa agttattgat ggattgggta 
               
               
                   
               
               
                 3361 
                 aatgctgttt gtgccttcta taataaaaag gtggagaatt ttacagtgtc tttctcagac 
               
               
                   
               
               
                 3421 
                 ggccgtgtgt tatgttacct gatccaccat taccatcctt gctatgtgcc atttgacgct 
               
               
                   
               
               
                 3481 
                 atatgtcagc gtactactca aactgtggaa tgtacgcaaa ctggttcagt ggtattaaat 
               
               
                   
               
               
                 3541 
                 tcatcatctg aatctgatga cagttctctg gatatgtcac ttaaagcatt tgatcatgaa 
               
               
                   
               
               
                 3601 
                 aatacttcag agctatacaa agagctccta gaaaatgaaa agaaaaattt tcacttggtt 
               
               
                   
               
               
                 3661 
                 aggtctgcag ttagagacct tggtggaata cctgctatga ttaatcattc agatatgtca 
               
               
                   
               
               
                 3721 
                 aatacaattc cagatgaaaa ggtggttatt acctatttgt catttctttg tgcaaggctt 
               
               
                   
               
               
                 3781 
                 ttggatcttc gtaaagaaat aagagctgct cgactcatac aaacaacatg gagaaaatat 
               
               
                   
               
               
                 3841 
                 aaactaaaaa cagatctcaa acgccatcag gagagagaga aagctgcaag aattattcaa 
               
               
                   
               
               
                 3901 
                 ttggctgtaa tcaattttct agcaaaacaa agattgagaa aaagagttaa tgcagcactc 
               
               
                   
               
               
                 3961 
                 gtcattcaga aatattggcg aagagtctta gcacagagaa aattattaat gttaaaaaag 
               
               
                   
               
               
                 4021 
                 gaaaagctgg aaaaagttca aaataaagca gcatcactta ttcagggata ttggagaaga 
               
               
                   
               
               
                 4081 
                 tattccacta gacaaagatt tctgaaattg aaatattatt caatcatcct gcaatctagg 
               
               
                   
               
               
                 4141 
                 ataagaatga taattgctgt tacatcttat aaacgatatc tttgggctac agttacaatt 
               
               
                   
               
               
                 4201 
                 cagaggcatt ggcgtgctta tttaagaaga aaacaagatc aacaaagata tgaaatgcta 
               
               
                   
               
               
                 4261 
                 aaatcatcaa ctcttataat ccaatctatg ttcagaaaat ggaagcaacg taaaatgcaa 
               
               
                   
               
               
                 4321 
                 tcacaagtaa aagctacagt aatattgcaa agagctttta gagaatggca tttaagaaaa 
               
               
                   
               
               
                 4381 
                 caagctaaag aagaaaattc tgctattatc atacaatcat ggtatagaat gcataaagaa 
               
               
                   
               
               
                 4441 
                 ttacggaagt atatttatat tagatcttgt gttgttatca ttcagaaaag atttcggtgc 
               
               
                   
               
               
                 4501 
                 tttcaagccc aaaagttata taaaagaaga aaagagtcca tactaaccat ccagaagtac 
               
               
                   
               
               
                 4561 
                 tacaaagcat atctgaaagg aaagattgag cgcaccaact atttgcagaa acgagctgca 
               
               
                   
               
               
                 4621 
                 gccattcaat tacaagctgc ttttaggaga ctgaaagctc ataatttatg tagacaaatt 
               
               
                   
               
               
                 4681 
                 agagctgctt gtgttattca gtcatactgg agaatgagac aagacagagt tcgattttta 
               
               
                   
               
               
                 4741 
                 aaccttaaga agactattat caaatttcag gcacatgtaa gaaaacatca acaacgacag 
               
               
                   
               
               
                 4801 
                 aaatataaga agatgaagaa agcagctgtt ataattcaga ctcatttccg agcttatatt 
               
               
                   
               
               
                 4861 
                 tttgccatga aagttctagc atcttaccag aaaacacgct ctgctgtcat tgtgctgcag 
               
               
                   
               
               
                 4921 
                 tctgcatata gagggatgca agccaggaaa atgtatattc acatcctcac atctgttata 
               
               
                   
               
               
                 4981 
                 aagattcaat catattatcg tgcttatgtt tctaaaaagg aatttttgag cctaaaaaat 
               
               
                   
               
               
                 5041 
                 gctacaataa aattgcagtc aactgttaag atgaaacaaa cacgtaaaca atatttgcat 
               
               
                   
               
               
                 5101 
                 ttaagagcag ctgcactatt tatccagcaa tgttaccgtt ccaaaaaaat agctgcacaa 
               
               
                   
               
               
                 5161 
                 aagagagaag agtatatgca gatgcgggaa tcttgtatca aactgcaagc atttgttaga 
               
               
                   
               
               
                 5221 
                 ggataccttg tccgaaagca gatgaggtta caaagaaaag ctgttatttc actacagtct 
               
               
                   
               
               
                 5281 
                 tatttcagaa tgagaaaggc tcggcagtat tatctgaaaa tgtataaagc aattattgtc 
               
               
                   
               
               
                 5341 
                 attcagaatt actatcatgc atacaaagca caggtcaatc agaggaagaa cttcttgcaa 
               
               
                   
               
               
                 5401 
                 gtcaaaaaag cagctacttg cttgcaagca gcttacagag gttataaagt acgccagcta 
               
               
                   
               
               
                 5461 
                 atcaaacaac aatctatagc tgctcttaaa attcagtctg cttttagagg ctataataaa 
               
               
                   
               
               
                 5521 
                 agggtaaaat atcaatctgt gcttcaatct ataataaaga ttcagagatg gtacagggcg 
               
               
                   
               
               
                 5581 
                 tacaagactc ttcatgatac aagaacacat tttttgaaga caaaggcagc tgtgatttcc 
               
               
                   
               
               
                 5641 
                 ctccagtctg cttatcgtgg ctggaaggtt cggaaacaga ttagaaggga acatcaagct 
               
               
                   
               
               
                 5701 
                 gccttgaaga ttcagtctgc ttttagaatg gccaaggccc agaaacagtt tagattgttt 
               
               
                   
               
               
                 5761 
                 aaaacagcag cattagtcat ccagcaaaat ttcagagcat ggactgcagg aaggaagcaa 
               
               
                   
               
               
                 5821 
                 tgtatggagt atattgaact ccgtcatgcg gtactggtgc ttcaatctat gtggaaggga 
               
               
                   
               
               
                 5881 
                 aaaacactga gaagacagct tcaaaggcaa cataaatgtg ctatcatcat acagtcatac 
               
               
                   
               
               
                 5941 
                 tatagaatgc atgtgcaaca aaagaagtgg aaaatcatga aaaaagctgc tcttctgatt 
               
               
                   
               
               
                 6001 
                 caaaagtatt atagggctta cagtattgga agagaacaga atcatttata tttgaaaaca 
               
               
                   
               
               
                 6061 
                 aaagcagctg tagtaacttt acagtcagct tatcgtggta tgaaagtgag aaaaagaata 
               
               
                   
               
               
                 6121 
                 aaggattgca acaaagcagc agtcactata cagtctaaat acagagctta caaaaccaaa 
               
               
                   
               
               
                 6181 
                 aagaaatatg caacctatag agcttcagct attataattc agagatggta tcgaggtatt 
               
               
                   
               
               
                 6241 
                 aaaattacaa accatcagca taaggagtat cttaatttga agaagacagc aattaaaatc 
               
               
                   
               
               
                 6301 
                 caatctgttt atagaggtat tagagttaga agacatattc aacacatgca cagggcagcc 
               
               
                   
               
               
                 6361 
                 acttttatta aagccatgtt taaaatgcat cagtcaagaa taagttacca tacaatgaga 
               
               
                   
               
               
                 6421 
                 aaagcagcta ttgttattca agtaagatgt agagcatatt atcaaggtaa aatgcagcgt 
               
               
                   
               
               
                 6481 
                 gaaaagtacc tgacaatttt gaaagctgtt aaagtccttc aggcaagttt tagaggagta 
               
               
                   
               
               
                 6541 
                 agagttagac ggactcttag aaagatgcag actgcagcaa cactcattca gtcaaactac 
               
               
                   
               
               
                 6601 
                 agaagataca gacagcaaac atactttaat aagttaaaga aaataacaaa aacagtacag 
               
               
                   
               
               
                 6661 
                 caaagatact gggcaatgaa agaaagaaac atacaatttc aaaggtataa caaactgagg 
               
               
                   
               
               
                 6721 
                 cattctgtaa tatacattca ggctattttt aggggaaaga aagctagaag acatttaaaa 
               
               
                   
               
               
                 6781 
                 atgatgcata tagccgcaac tctcattcag aggagattta gaactctaat gatgagaaga 
               
               
                   
               
               
                 6841 
                 agattcctct ctctcaagaa aactgctatt ttgattcaga gaaaatatcg ggcacatctt 
               
               
                   
               
               
                 6901 
                 tgtacaaagc atcacttaca gttccttcag gtacaaaatg cagttattaa aatccagtca 
               
               
                   
               
               
                 6961 
                 tcatacagaa gatggatgat aaggaaaagg atgcgagaga tgcacagggc tgctactttc 
               
               
                   
               
               
                 7021 
                 atccagtcta ctttcagaat gcacagatta catatgagat atcgagcttt gaaacaggcc 
               
               
                   
               
               
                 7081 
                 tccgttgtga tccaacagca ataccaagca aatagagctg caaaactgca gaggcagcat 
               
               
                   
               
               
                 7141 
                 tatctcagac aaagacactc tgctgtgatc cttcaggctg cattcagggg tatgaaaact 
               
               
                   
               
               
                 7201 
                 agaagacatt tgaagagtat gcattcctct gcaaccctta ttcagagtag gtttagatca 
               
               
                   
               
               
                 7261 
                 ttactggtga ggagaagatt catttccctc aaaaaagcta ctatttttgt tcagaggaaa 
               
               
                   
               
               
                 7321 
                 tatcgagcca ccatttgtgc caaacataaa ttgtaccaat tcttgcactt aagaaaggca 
               
               
                   
               
               
                 7381 
                 gccattacaa tacagtcatc ttacagaaga ctgatggtaa agaagaagtt acaagaaatg 
               
               
                   
               
               
                 7441 
                 caaagggctg cagttctcat tcaggctact ttcaggatgc acagaacata tattacattt 
               
               
                   
               
               
                 7501 
                 cagacttgga aacatgcttc aattctaatt cagcaacatt atcgaacata tagagctgca 
               
               
                   
               
               
                 7561 
                 aaattgcaaa gagaaaatta tatcagacaa tggcattctg ctgtggttat tcaggctgca 
               
               
                   
               
               
                 7621 
                 tataaaggaa tgaaagcaag acaactttta agggaaaaac acaaagcttc tatcgtaata 
               
               
                   
               
               
                 7681 
                 caaagcacct acagaatgta taggcagtat tgtttctacc aaaagcttca gtgggctaca 
               
               
                   
               
               
                 7741 
                 aaaatcatac aagaaaaata tagagcaaat aaaaagaaac agaaagtatt tcaacacaat 
               
               
                   
               
               
                 7801 
                 gaacttaaga aagagacttg tgttcaggca ggttttcagg acatgaacat aaaaaaacag 
               
               
                   
               
               
                 7861 
                 attcaggaac agcaccaggc tgccattatt attcagaagc attgtaaagc ctttaaaata 
               
               
                   
               
               
                 7921 
                 aggaagcatt atctccacct tagagcaaca gtagtttcta ttcaaagaag atacagaaaa 
               
               
                   
               
               
                 7981 
                 ctaactgcag tgcgtaccca agcagttatt tgtatacagt cttattacag aggctttaaa 
               
               
                   
               
               
                 8041 
                 gtacgaaagg atattcaaaa tatgcaccgg gctgccacac taattcagtc attctatcga 
               
               
                   
               
               
                 8101 
                 atgcacaggg ccaaagttga ttatgaaaca aagaaaactg caattgtggt tatacagaat 
               
               
                   
               
               
                 8161 
                 tattataggt tgtatgttag agtaaaaaca gaaagaaaaa actttttagc agttcagaaa 
               
               
                   
               
               
                 8221 
                 tctgtacgaa ctattcaggc tgcttttaga ggcatgaaag ttagacaaaa attgaaaaat 
               
               
                   
               
               
                 8281 
                 gtatcagagg aaaagatggc agccattgtt aaccaatctg cactctgctg ttacagaagt 
               
               
                   
               
               
                 8341 
                 aaaactcagt atgaagctgt tcaaagtgaa ggtgttatga ttcaagagtg gtataaagct 
               
               
                   
               
               
                 8401 
                 tctggccttg cttgttcaca ggaagcagag tatcattctc aaagtagggc tgcagtaaca 
               
               
                   
               
               
                 8461 
                 attcaaaaag ctttttgtag aatggtcaca agaaaactgg aaacacagaa atgtgctgcc 
               
               
                   
               
               
                 8521 
                 ctacggattc agttcttcct tcagatggct gtgtatcgga gaagatttgt tcagcagaaa 
               
               
                   
               
               
                 8581 
                 agagctgcta tcactttaca gcattatttt aggacgtggc aaaccagaaa acagttttta 
               
               
                   
               
               
                 8641 
                 ctatatagaa aagcagcagt ggttttacaa aatcactaca gagcatttct gtctgcaaaa 
               
               
                   
               
               
                 8701 
                 catcaaagac aagtctattt acagatcaga agcagtgtta tcattattca agctagaagt 
               
               
                   
               
               
                 8761 
                 aaaggattta tacagaaacg gaagtttcag gaaattaaaa atagcaccat aaaaattcag 
               
               
                   
               
               
                 8821 
                 gctatgtgga ggagatatag agccaagaaa tatttatgta aagtgaaagc tgcctgcaag 
               
               
                   
               
               
                 8881 
                 attcaagcct ggtatagatg ttggagagca cacaaagaat atctagctat attaaaagct 
               
               
                   
               
               
                 8941 
                 gttaaaatta ttcaaggttg cttctatacc aaactagaga gaacacggtt tttgaatgtg 
               
               
                   
               
               
                 9001 
                 agagcatcag caattatcat tcagagaaaa tggagagcta tacttcctgc aaagatagct 
               
               
                   
               
               
                 9061 
                 catgaacact tcttaatgat aaaaagacat cgagctgctt gtttgatcca agcacattat 
               
               
                   
               
               
                 9121 
                 agaggatata aaggaaggca ggtctttctt cggcagaaat ctgctgcttt gatcatacaa 
               
               
                   
               
               
                 9181 
                 aaatatatac gagccaggga ggctggaaag catgaaagga taaaatatat tgaatttaaa 
               
               
                   
               
               
                 9241 
                 aaatctacag ttatcctaca agcactggtg cgtggttggc tagtacgaaa aagattttta 
               
               
                   
               
               
                 9301 
                 gaacagagag ccaaaattcg acttcttcac ttcactgcag ctgcatatta tcacctgaat 
               
               
                   
               
               
                 9361 
                 gctgttagaa ttcaaagagc ctataaactt tacctggctg tgaagaatgc taacaagcag 
               
               
                   
               
               
                 9421 
                 gttaattcag tcatctgtat tcagagatgg tttcgagcaa gattacaaga aaagagattt 
               
               
                   
               
               
                 9481 
                 attcagaaat atcatagcat caaaaagatt gagcatgaag gtcaagaatg tctgagccag 
               
               
                   
               
               
                 9541 
                 cgaaataggg ctgcatcagt aatacagaaa gcagtgcgcc attttctcct ccgtaaaaag 
               
               
                   
               
               
                 9601 
                 caggaaaaat tcactagtgg aatcattaaa attcaggcat tatggagagg ctattcttgg 
               
               
                   
               
               
                 9661 
                 aggaagaaaa atgattgtac aaaaattaaa gctatacgac taagtcttca agttgttaat 
               
               
                   
               
               
                 9721 
                 agggagattc gagaagaaaa caaactctac aaaagaactg cacttgcact tcattacctt 
               
               
                   
               
               
                 9781 
                 ttgacatata agcacctttc tgccattctt gaggccttaa aacacctaga ggtagttact 
               
               
                   
               
               
                 9841 
                 agattgtctc cactttgttg tgagaacatg gcccagagtg gagcaatttc taaaatattt 
               
               
                   
               
               
                 9901 
                 gttttgatcc gaagttgtaa tcgcagtatt ccttgtatgg aagtcatcag atatgctgtg 
               
               
                   
               
               
                 9961 
                 caagtcttgc ttaatgtatc taagtatgag aaaactactt cagcagttta tgatgtagaa 
               
               
                   
               
               
                 10021 
                 aattgtatag atatactatt ggagcttttg cagatatacc gagaaaagcc tggtaataaa 
               
               
                   
               
               
                 10081 
                 gttgcagaca aaggcggaag catttttaca aaaacttgtt gtttgttggc tattttactg 
               
               
                   
               
               
                 10141 
                 aagacaacaa atagagcctc tgatgtacga agtaggtcca aagttgttga ccgtatttac 
               
               
                   
               
               
                 10201 
                 agtctctaca aacttacagc tcataaacat aaaatgaata ctgaaagaat actttacaag 
               
               
                   
               
               
                 10261 
                 caaaagaaga attcttctat aagcattcct tttatcccag aaacacctgt aaggaccaga 
               
               
                   
               
               
                 10321 
                 atagtttcaa gacttaagcc agattgggtt ttgagaagag ataacatgga agaaatcaca 
               
               
                   
               
               
                 10381 
                 aatcccctgc aagctattca aatggtgatg gatacgcttg gcattcctta ttag 
               
               
                   
               
             
          
           
               
                 36: NM_002735   ACBD3 
                   
               
             
          
           
               
                 1 
                 atacgtggct gccgtctgtc cccgctgagg aggtgcagca gccggagatg gcggcggtgc 
                   
               
               
                   
               
               
                 61 
                 tgaacgcaga gcgactcgag gtgtccgtcg acggcctcac gctcagcccg gacccggagg 
               
               
                   
               
               
                 121 
                 agcggcctgg ggcggagggc gccccgctgc tgccgccacc gctgccaccg ccctcgccac 
               
               
                   
               
               
                 181 
                 ctggatccgg tcgcggcccg ggcgcctcag gggagcagcc cgagcccggg gaggcggcgg 
               
               
                   
               
               
                 241 
                 ctgggggcgc ggcggaggag gcgcggcggc tggagcagcg ctggggtttc ggcctggagg 
               
               
                   
               
               
                 301 
                 agttgtacgg cctggcactg cgcttcttca aagaaaaaga tggcaaagca tttcatccaa 
               
               
                   
               
               
                 361 
                 cttatgaaga aaaattgaag cttgtggcac tgcataagca agttcttatg ggcccatata 
               
               
                   
               
               
                 421 
                 atccagacac ttgtcctgag gttggattct ttgatgtgtt ggggaatgac aggaggagag 
               
               
                   
               
               
                 481 
                 aatgggcagc cctgggaaac atgtctaaag aggatgccat ggtggagttt gtcaagctct 
               
               
                   
               
               
                 541 
                 taaataggtg ttgccatctc ttttcaacat atgttgcgtc ccacaaaata gagaaggaag 
               
               
                   
               
               
                 601 
                 agcaagaaaa aaaaaggaag gaggaagagg agcgaaggcg gcgtgaagag gaagaaagag 
               
               
                   
               
               
                 661 
                 aacgtctgca aaaggaggaa gagaaacgta ggagagaaga agaggaaagg cttcgacggg 
               
               
                   
               
               
                 721 
                 aggaagagga aaggagacgg atagaagaag aaaggcttcg gttggagcag caaaagcagc 
               
               
                   
               
               
                 781 
                 agataatggc agctttaaac tcccagactg ccgtgcagtt ccagcagtat gcagcccaac 
               
               
                   
               
               
                 841 
                 agtatccagg gaactacgaa cagcagcaaa ttctcatccg ccagttgcag gagcaacact 
               
               
                   
               
               
                 901 
                 atcagcagta catgcagcag ttgtatcaag tccagcttgc acagcaacag gcagcattac 
               
               
                   
               
               
                 961 
                 agaaacaaca ggaagtagta gtggctgggt cttccttgcc tacatcatca aaagtgaatg 
               
               
                   
               
               
                 1021 
                 caactgtacc aagtaatatg atgtcagtta atggacaggc caaaacacac actgacagct 
               
               
                   
               
               
                 1081 
                 ccgaaaaaga actggaacca gaagctgcag aagaagccct ggagaatgga ccaaaagaat 
               
               
                   
               
               
                 1141 
                 ctcttccagt aatagcagct ccatccatgt ggacacgacc tcagatcaaa gacttcaaag 
               
               
                   
               
               
                 1201 
                 agaagattca gcaggatgca gattccgtga ttacagtggg ccgaggagaa gtggtcactg 
               
               
                   
               
               
                 1261 
                 ttcgagtacc cacccatgaa gaaggatcat atctcttttg ggaatttgcc acagacaatt 
               
               
                   
               
               
                 1321 
                 atgacattgg gtttggggtg tattttgaat ggacagactc tccaaacact gctgtcagcg 
               
               
                   
               
               
                 1381 
                 tgcatgtcag tgagtccagc gatgacgacg aggaggaaga agaaaacatc ggttgtgaag 
               
               
                   
               
               
                 1441 
                 agaaagccaa aaagaatgcc aacaagcctt tgctggatga gattgtgcct gtgtaccgac 
               
               
                   
               
               
                 1501 
                 gggactgtca tgaggaggtg tatgctggca gccatcaata tccagggaga ggagtctatc 
               
               
                   
               
               
                 1561 
                 tcctcaagtt tgacaactcc tactctttgt ggcggtcaaa atcagtctac tacagagtct 
               
               
                   
               
               
                 1621 
                 attatactag ataaaaatgt tgttacaaag tctggagtct agggttgggc agaagatgac 
               
               
                   
               
               
                 1681 
                 atttaatttg gaaatttctt tttacttttg tggagcatta gagtcacagt ttaccttatt 
               
               
                   
               
               
                 1741 
                 gatattggtc tgatggtttg tgaactcttg ctgggaatca aaatttcctt gagactcttt 
               
               
                   
               
               
                 1801 
                 agcattcata ctttggggtt aaaggagatt cctcagactc atccagccct tgggtgctga 
               
               
                   
               
               
                 1861 
                 ccagcagagt cactagtgga tgctgaagtt acatgagcta catgttaaat atttaaagtc 
               
               
                   
               
               
                 1921 
                 tccaaaataa aacaccccaa cgttgacctt acccggctga tggttagccc cttgctgcct 
               
               
                   
               
               
                 1981 
                 gctccatgtg tcttatgaga gcccgtagtt acagtgtcct ctaatttgaa atccataagt 
               
               
                   
               
               
                 2041 
                 taacaagtct atatcaggtg cagctggctt tgattaaagg ccatttttaa aacttaaaaa 
               
               
                   
               
               
                 2101 
                 ctcaacacct cacagattat aatagaaaaa gaaatggcct cagtttgatc tcgttcagaa 
               
               
                   
               
               
                 2161 
                 tgacccagat tgtttctgct ttgggtgcag ctgtttagtt cagagttata ttacagagaa 
               
               
                   
               
               
                 2221 
                 ttattttctg agataatctt aaactagaat gttcaaaact aattgataat tgaagtatca 
               
               
                   
               
               
                 2281 
                 agatacgtag aacacctcag agatttttct tcaggaactt ccacaaactt tgaatccttg 
               
               
                   
               
               
                 2341 
                 tatctttatt tggtattcat actactagta gcaaaataca ggttttttgt tttgttttgt 
               
               
                   
               
               
                 2401 
                 tttgttttgg cttcatagag tatctcaaat tgaaactttt ctgcacaaag aataaaatta 
               
               
                   
               
               
                 2461 
                 aggattttat aaactcaaat tggcacctac tgaattaaaa tacataaaat catttaaata 
               
               
                   
               
               
                 2521 
                 taattcagca tatgggaagt aacattgcac taatatggaa atcactgcca gagacagtct 
               
               
                   
               
               
                 2581 
                 attttctttt aatttgttac tacttagtca caaaccccac attattccag tttggaatta 
               
               
                   
               
               
                 2641 
                 cttattaagg agaattggaa atacatatgc ccatgcttaa attttatagc tttaatttgt 
               
               
                   
               
               
                 2701 
                 gttatttctt tattgacggg aagaggtaca tctttttttc cttactgaaa acaaatatgg 
               
               
                   
               
               
                 2761 
                 attaattgcc tcaaatttgt ataagtgatt ggctagtgat tcttgttttc agaagggaga 
               
               
                   
               
               
                 2821 
                 gtggtataga tagaaaatga caaagatggc aatatacact taatgttgtt attgtatgtt 
               
               
                   
               
               
                 2881 
                 gttactgaag tacttagatt tttaaaattt caaatcctaa atcacttctt gtaggagggt 
               
               
                   
               
               
                 2941 
                 tttcattaac tgcagtatat acagttcact acatatgggt tgtttgagtt ttttgtgtgc 
               
               
                   
               
               
                 3001 
                 tgtatttctt tctgtttttt aatacctggt tttgtacata tctaactctg ttctcttttg 
               
               
                   
               
               
                 3061 
                 gttgttcaga aactggattt tttttttctt aagcagtgct taatttgtgt tttttaattt 
               
               
                   
               
               
                 3121 
                 tgattcagaa gtagtcccag ctcataggtg ttcatactgt tacatccaga acatttgtca 
               
               
                   
               
               
                 3181 
                 ggctctctgt cagctttcat gtacatatgg tatagaaacc atggagttag gcacttcctg 
               
               
                   
               
               
                 3241 
                 gatttttttt ttatgagaaa aatactgtat ttaaaatgta aaataaactt ttaaaaagca 
               
               
                   
               
               
                 3301 
                 ggcactaata tatatttctt ccagcctttg attacaaatt tgtccttgca catgttaaga 
               
               
                   
               
               
                 3361 
                 tgaattatct cctaaaaata tcattgttct tgggagcagt gtatgttact ttacatagca 
               
               
                   
               
               
                 3421 
                 gcggttcctg tcatgtgttc atgtcagaat atttttggtt ttaaactttc ttattgcctt 
               
               
                   
               
               
                 3481 
                 tggctgttga ttagtacagt acaagtgcga tttcaaaaag atcttgaaag taatatattt 
               
               
                   
               
               
                 3541 
                 aatcaattaa aatgtttatc tgtaaaaaaa aaaaaaaaaa a 
               
               
                   
               
             
          
           
               
                 37: AA160544   ZNF325 
                   
               
             
          
           
               
                 1 
                 tttttttaca gttttcaaat attttactga aaatgcatat tgtacaatta atgtataatg 
                   
               
               
                   
               
               
                 61 
                 acacaccagt gtgagaaacc tccataggta tcatttccac aaatatgcta tgaatataga 
               
               
                   
               
               
                 121 
                 gttcctacac aaaactatac aacttaccag atgtaattcc tgttacgtac catactcaca 
               
               
                   
               
               
                 181 
                 atcgtcttga agaatatgga gaaaaagtgc tgagtgacaa aaacaggagc catgtgtgat 
               
               
                   
               
               
                 241 
                 tttaataaat ggaaaacacg gcatttcagc tcagtggtaa agcagtaaac caatcagatg 
               
               
                   
               
               
                 301 
                 cttagctatc aagtaatcat gtgagaggaa acagaattag atcctacctc atactatatg 
               
               
                   
               
               
                 361 
                 ttgtcagcta acactgtagc agtggtatat gaatcactaa attacctcca acaaaatgta 
               
               
                   
               
               
                 421 
                 ttcctgtatt gaaaaaagga ggtatggcca acattgtgtc acgttccaag gtgaattttg 
               
               
                   
               
               
                 481 
                 cggtcacgat atgacgttca ggaagctact tttattgttc agttgatttc tatgctcaac 
               
               
                   
               
               
                 541 
                 tattaggtca attccgaaat aatcncatat cacagctaaa ataatgncta ccaagtcnct 
               
               
                   
               
               
                 601 
                 ctgactgct 
               
               
                   
               
             
          
           
               
                 38: AK057653   LOC285513 
                   
               
             
          
           
               
                 1 
                 ctgttagcaa tgcttcctga tgttgtgcgt ggcccttttt ggttgattct ctccaaattc 
                   
               
               
                   
               
               
                 61 
                 gggtcagctg ctgccacctg gcaaataaca gaggatatgc tgaatctcct gtccatcctt 
               
               
                   
               
               
                 121 
                 gtaacgatat ccttcttaat gaaattcttc aactggctga gcaattacaa atgtcatctg 
               
               
                   
               
               
                 181 
                 tccagacaca tgggcttaag gatgtctaca aaattttaga catttttgca aatgggaaaa 
               
               
                   
               
               
                 241 
                 aaaatagtct tgtaaatact gaaacagatt tccatgaact ttatcctact cttggaaaga 
               
               
                   
               
               
                 301 
                 aaacaattct ccttggctgc agaaatcaaa taagctgggt ttgcaatgac caaggacata 
               
               
                   
               
               
                 361 
                 aatgaagatg gattgaagtg gaaaaattct gtctcccaag tgatcagtga catctgccag 
               
               
                   
               
               
                 421 
                 aggtcattac agctactttt aactgtgaac agtcaccagc taaactactc acttgccaca 
               
               
                   
               
               
                 481 
                 acaaaataac ctctctcaaa gtaaatccag tgcatctgta tatatgtgta gatagcagca 
               
               
                   
               
               
                 541 
                 acaaacaatc ctgaaacatt atttttggct gttaggtaag taaacgtgat gataattata 
               
               
                   
               
               
                 601 
                 aacaacattc aaataacctt ggaccttggt gaaatgactt gtggtggcca gaatggtgca 
               
               
                   
               
               
                 661 
                 acaagatgtt atttgcaagt ttttttaaga cacaaatatc tcagatacta ataatgagaa 
               
               
                   
               
               
                 721 
                 taaagactgt tgaatatgaa attaaagcca agcaataatg tgccaaaaag aggcagttat 
               
               
                   
               
               
                 781 
                 accagcaaat gcatctatta tgggcacacc attatataat gatggtttgc tttatgaaga 
               
               
                   
               
               
                 841 
                 ctgactgtaa cccacaggat aaaataagca aaggcatagt ttctgctttc ttcctggaaa 
               
               
                   
               
               
                 901 
                 aacttgttta gaagcttcat aaagaggtac agcactaatg agcattagtc aggatacagt 
               
               
                   
               
               
                 961 
                 tggcatctat gtttttatgt gagcccagag ggaagaggag ccactcaaag tcttgctggt 
               
               
                   
               
               
                 1021 
                 ttaaaactca agacagctgc aaccagaagt tttgttgaaa tggagacttt aaacttatgg 
               
               
                   
               
               
                 1081 
                 taattactct ttctggacac tagcatgtag aaagcaattc agttaactct gcccagagga 
               
               
                   
               
               
                 1141 
                 ttaccagctt tagctgtgaa aaaatgggct cccggatgta aaatcactaa aacatgagat 
               
               
                   
               
               
                 1201 
                 cttgtatcca aagaggcttc aaatgatgcc ttacagaaaa cgatgctcca gatgggcact 
               
               
                   
               
               
                 1261 
                 tctaaatgct aactcttcat caagtatctt tctggattca agctcaaaat taattggctg 
               
               
                   
               
               
                 1321 
                 caaaatagta ggaataaaaa tcacatattt tacactttag aaaaggatat tgatgatcaa 
               
               
                   
               
               
                 1381 
                 cctgcatggt gataattatg atgagatacc ccagtgattt aatgatgtta gaaagaatta 
               
               
                   
               
               
                 1441 
                 aatgggagag aattgctaac agctttcttg atctcttaac tatggagatg tcattcattt 
               
               
                   
               
               
                 1501 
                 atttctgggg tgaaaattat agcttgcttt ttgacattgc tgctagtatt gttctttgtt 
               
               
                   
               
               
                 1561 
                 gctttaaaaa ttgtctctct ttagaaaaac tcttgagcag ttaaacagtt ctttttctga 
               
               
                   
               
               
                 1621 
                 ttcatatcat tgcttttaat aacatgtaaa ggctgtgtgt agagcaaact atataaaatg 
               
               
                   
               
               
                 1681 
                 agtagaaagg gcttgctcat gttaattggc atccttgatg attttagttg agattcctta 
               
               
                   
               
               
                 1741 
                 acatttattt tagatcacat ctttacgtaa cttatttttc ctaatgtttt ccatcgtgtc 
               
               
                   
               
               
                 1801 
                 ttaaaatgat gctggtatat caggagattg cagtattata gtcatactcc ccaatcccta 
               
               
                   
               
               
                 1861 
                 gaggagagga aagactaatt cttgttttaa gggcccctgg agataccttt tattaaggtt 
               
               
                   
               
               
                 1921 
                 gaaaaaggtc aacacagcct gaaaataaga aaaatatata ctagcaatta ctaattttct 
               
               
                   
               
               
                 1981 
                 aaatgtgtgt atctctgctg tactaatgtg tgaacaatat gtcgtgcata atactgtagc 
               
               
                   
               
               
                 2041 
                 tggtcgtggt atgtcaatac attctgtgag tgtgtacagt ctgagtgatc agttttctat 
               
               
                   
               
               
                 2101 
                 ttttatgtgt aaaaaaaata acttgtcgta tcccatttaa aggccaattt ctgtattcag 
               
               
                   
               
               
                 2161 
                 gcaggcatat gtacatacat gaataaagcc aacaaaagtg tgcacatgta ttcagt 
               
               
                   
               
             
          
           
               
                 39: NM_003310   TSSC1 
                   
               
             
          
           
               
                 1 
                 aattcggcac gagaagactt ccagtttgga gtcgtttgct gcggggaggg aatgaatggg 
                   
               
               
                   
               
               
                 61 
                 cgctgggaac acgcccgcga ggtggggacg cgccggccgt agcgaggtcc ttagcgtgtg 
               
               
                   
               
               
                 121 
                 agtggccggg gtcgggtcgc ttccccgcag catggaggac gatgcaccag tgatctacgg 
               
               
                   
               
               
                 181 
                 gctggagttc caggcacgtg ccttaacacc tcaaactgca gaaacagatg ccattcggtt 
               
               
                   
               
               
                 241 
                 tttggttggg acgcagtctc ttaaatatga taatcagatc catatcatag attttgacga 
               
               
                   
               
               
                 301 
                 tgaaaacaac attataaata aaaatgtcct cctccatcaa gcgggtgaaa tctggcatat 
               
               
                   
               
               
                 361 
                 tagcgctagc cctgcagaca gaggtgtgct gacgacctgc tacaacagaa cttcagacag 
               
               
                   
               
               
                 421 
                 caaagtcctg acatgtgcag ccgtgtggag gatgccgaag gaattggaat caggcagcca 
               
               
                   
               
               
                 481 
                 cgagtcccct gatgattcat ccagcactgc acagaccctg gagctgctct gtcaccttga 
               
               
                   
               
               
                 541 
                 caacacagcc catggcaaca tggcctgtgt cgtgtgggag ccaatgggag atgggaagaa 
               
               
                   
               
               
                 601 
                 aatcatttcc ttggctgata accatatcct gctgtgggat ttacaggaaa gctcgagcca 
               
               
                   
               
               
                 661 
                 ggctgtgctg gccagctcag cgtccctgga agggaaggga caactgaagt tcacctcagg 
               
               
                   
               
               
                 721 
                 acggtggagc ccacatcata actgcaccca ggtggccaca gcgaacgaca ccaccctccg 
               
               
                   
               
               
                 781 
                 tggctgggac acccggagca tgagccagat ctactgcata gagaatgccc acggacagct 
               
               
                   
               
               
                 841 
                 ggtgcgggac cttgacttta atcccaataa gcagtactac ttggccagct gcggagacga 
               
               
                   
               
               
                 901 
                 ctgtaaggtg aagttctggg acacccgaaa tgtcaccgaa cccgtgaaga ccctggagga 
               
               
                   
               
               
                 961 
                 gcactcccac tgggtgtgga acgtccgcta caaccactct catgaccagc tggtcctcac 
               
               
                   
               
               
                 1021 
                 gggcagcagt gacagcagag tcatcctttc caacatggtg tccatctcgt cggagccctt 
               
               
                   
               
               
                 1081 
                 cggccacttg gtagacgacg atgacatcag tgaccaggag gaccaccgtt ctgaagagaa 
               
               
                   
               
               
                 1141 
                 gagcaaggag cccctgcagg acaacgtgat cgccacctac gaggagcacg aggacagcgt 
               
               
                   
               
               
                 1201 
                 ctatgccgtg gactggtcct cggctgaccc gtggctgttt gcctccctga gctatgacgg 
               
               
                   
               
               
                 1261 
                 gaggctcgtg atcaacaggg tgcccagggc cctgaagtac cacatcctgc tatgactccc 
               
               
                   
               
               
                 1321 
                 gggcctgggt tatccaggtc ccattgagtg gttttcctct tggcagattc tcaaacagtc 
               
               
                   
               
               
                 1381 
                 gcagctcttt ggaggtgact cgtgttccag gtggatccct ctctgggaga gccgctgttc 
               
               
                   
               
               
                 1441 
                 ccttcctgta gcagcagcat ttatgaatgg ggtgaatggg gctattgtcg acggcacagc 
               
               
                   
               
               
                 1501 
                 taatgcccga acccagcccc tgtcggcaga gacagagccc cacattatta tgtgaataac 
               
               
                   
               
               
                 1561 
                 aatgttttct gttttaaggg tgtcaggagt ttcgcttttt aaaaaaatgt ctgttcctgc 
               
               
                   
               
               
                 1621 
                 agtagtaact cttctttctc ttgagagtaa aaaatgaaat aaaataaatc cacgctgaca 
               
               
                   
               
               
                 1681 
                 aaaaaaaaaa aaaaaaaaaa aaaaa 
               
               
                   
               
             
          
           
               
                 40: BC007451   XAB1 
                   
               
             
          
           
               
                 1 
                 gaggaagatg gcggcgtccg cagctgccgc tgagctccag gcttctgggg gtccgcggca 
                   
               
               
                   
               
               
                 61 
                 cccagtgtgt ctgttggtgt tgggaatggc gggatccggg aaaaccactt ttgtacagag 
               
               
                   
               
               
                 121 
                 gctcacagga cacctgcatg cccaaggcac tccaccgtat gtgatcaacc tggatccagc 
               
               
                   
               
               
                 181 
                 agtacatgaa gttccctttc ctgccaatat tgatattcgt gatactgtaa agtataaaga 
               
               
                   
               
               
                 241 
                 agtaatgaaa caatatggac ttggacccaa tggcggcata gtgacctcac tcaatctctt 
               
               
                   
               
               
                 301 
                 tgctaccaga tttgatcagg tgatgaaatt tattgagaag gcccagaaca tgtccaaata 
               
               
                   
               
               
                 361 
                 tgtgttgatt gacacacctg gacagattga ggtattcacc tggtcagctt ctgggacaat 
               
               
                   
               
               
                 421 
                 tatcactgaa gcccttgcat cctcatttcc aacagttgtc atctatgtaa tggacacatc 
               
               
                   
               
               
                 481 
                 gagaagtacc aacccagtga ccttcatgtc caacatgctc tatgcctgca gcatcttata 
               
               
                   
               
               
                 541 
                 caaaaccaag ctgcctttca ttgtggtcat gaataaaact gacatcattg accacagctt 
               
               
                   
               
               
                 601 
                 tgcagtggaa tggatgcagg attttgaggc tttccaagat gccttgaatc aagagactac 
               
               
                   
               
               
                 661 
                 atacgtcagt aacctgactc gttcaatgag cctggtgtta gatgagtttt acagctcact 
               
               
                   
               
               
                 721 
                 cagggtggtg ggtgtctctg ctgttctggg tactggatta gatgaactct ttgtgcaagt 
               
               
                   
               
               
                 781 
                 taccagtgct gccgaagaat atgaaaggga gtatcgtcct gaatatgaac gtctgaaaaa 
               
               
                   
               
               
                 841 
                 atcactggcc aacgcagaga gccaacagca gagagaacaa ctggaacgcc ttcgaaaaga 
               
               
                   
               
               
                 901 
                 tatgggttct gtagccttgg atgcagggac tgccaaagac agcttatctc ctgtgctgca 
               
               
                   
               
               
                 961 
                 cccttctgat ttgatcctga ctcgaggaac cttggatgaa gaggatgagg aagcagacag 
               
               
                   
               
               
                 1021 
                 cgatactgat gacattgacc acagagttac agaggaaagc catgaagagc cagcattcca 
               
               
                   
               
               
                 1081 
                 gaattttatg caagaatcga tggcacaata ctggaagaga aacaataaat aggagacttt 
               
               
                   
               
               
                 1141 
                 agcacacttc acttgtttct agaagtccag aattttggac ctccacgtga aagaactgtt 
               
               
                   
               
               
                 1201 
                 cttacctctg aactgggggc tcccataagg gataattttc ctcagagtag caaagtttct 
               
               
                   
               
               
                 1261 
                 cttattagag aaatcttgtg actcagatga agtcagggat agaagaccct tggacctggc 
               
               
                   
               
               
                 1321 
                 aggttaatgc tgattattcc ttggcctttc ccttgtattt atgcaaggaa ggatatactg 
               
               
                   
               
               
                 1381 
                 agctgatact gttccaagcc tacaacttca agttttatca tttgaactca agtacttttg 
               
               
                   
               
               
                 1441 
                 ctgctgagga atggaatcaa aagaacgtag tctcctggtg accacctcag atctctatta 
               
               
                   
               
               
                 1501 
                 ttaggctaga tgtatagcct ctactccccc agcttcttgc tcttgaccct gcactgtaag 
               
               
                   
               
               
                 1561 
                 ttgcccttct attagcagcc aaggaaaagg gaaacatgag cttatccaga acggtggcag 
               
               
                   
               
               
                 1621 
                 agtctccttg gcaatcaacc aacgttgcta tgaaatatgc ctcacactgt atagctcatt 
               
               
                   
               
               
                 1681 
                 ataggacgtc aggtttgttg aaaaaagtgg gcaagacatg attaatgaat cagaatcctg 
               
               
                   
               
               
                 1741 
                 tttcattggt gacttggata aagacttttt aattttaaaa aaaaaaaaaa aaaaaaaaaa 
               
               
                   
               
             
          
           
               
                 41: BC035467   HNLF 
                   
               
             
          
           
               
                 1 
                 ggctgaggcg cgatggcagg tgtcggggct gggcctctgc gggcgatggg gcggcaggcc 
                   
               
               
                   
               
               
                 61 
                 ctgctgcttc tcgcgctgtg cgccacaggc gcccaggggc tctacttcca catcggcgag 
               
               
                   
               
               
                 121 
                 accgagaagc gctgtttcat cgaggaaatc cccgacgaga ccatggtcat cggcaactat 
               
               
                   
               
               
                 181 
                 cgtacccaga tgtgggataa gcagaaggag gtcttcctgc cctcgacccc tggcctgggc 
               
               
                   
               
               
                 241 
                 atgcacgtgg aagtgaagga ccccgacggc aaggtggtgc tgtcccggca gtacggctcg 
               
               
                   
               
               
                 301 
                 gagggccgct tcacgttcac ctcccacacg cccggtgacc atcaaatctg tctgcactcc 
               
               
                   
               
               
                 361 
                 aattctacca ggatggctct cttcgctggt ggcaaactgc gtgtgcatct cgacatccag 
               
               
                   
               
               
                 421 
                 gttggggagc atgccaacaa ctaccctgag attgctgcaa aagataagct gacggagcta 
               
               
                   
               
               
                 481 
                 cagctccgcg cccgccagtt gcttgatcag gtggaacaga ttcagaagga gcaggattac 
               
               
                   
               
               
                 541 
                 caaaggtatc gtgaagagcg cttccgactg acgagcgaga gcaccaacca gagggtccta 
               
               
                   
               
               
                 601 
                 tggtggtcca ttgctcagac tgtcatcctc atcctcactg gcatctggca gatgcgtcac 
               
               
                   
               
               
                 661 
                 ctcaagagct tctttgaggc caagaagctg gtgtagtgcc ctctttgtat gacccttcct 
               
               
                   
               
               
                 721 
                 ttttacctca tttatttggt actttcccca cacagtcctt tatccacctg gatttttagg 
               
               
                   
               
               
                 781 
                 gaaaaaaatg aaaaagaata agtcacattg gttccatggc cacaaaccat tcagatcagc 
               
               
                   
               
               
                 841 
                 cacttgctga ccctggttct taaggacaca tgacattagt ccaatctttc aaaatcttgt 
               
               
                   
               
               
                 901 
                 cttagggctt gtgaggaatc agaactaacc caggactcag tcctgcttct tttgcctcga 
               
               
                   
               
               
                 961 
                 gtgattttcc tctgtttttc actaaataag caaatgaaaa ctctctccat taccttctgc 
               
               
                   
               
               
                 1021 
                 tttctctttg tccacttacg cagtaggtga ctggcatgtg ccacagagca ggccctgcct 
               
               
                   
               
               
                 1081 
                 cactgtctgc tggtcagttc tgggttcact taatggcttt gtgaatgtaa ataaggggca 
               
               
                   
               
               
                 1141 
                 ggtcttggcc ctagaggatt gagatgtttt tctatatctt agaactattt ttggataaat 
               
               
                   
               
               
                 1201 
                 tatatatttt ccttcctagt agaagtgtta ctgcctgtaa ctagctcaaa ataccaatgc 
               
               
                   
               
               
                 1261 
                 agtttctgca ttctgggttt tgtttttcct tttttttttt tttttttttt ttttgagttt 
               
               
                   
               
               
                 1321 
                 tgctctcgtc gcccaggctg gagtgcaatg gcgtgatctc agctcactgg caacatctgc 
               
               
                   
               
               
                 1381 
                 ctcccgggtt caaatgattc tcctgcctca gtctcctgag tagctgggat tacaggtgcc 
               
               
                   
               
               
                 1441 
                 cgccaccacg ctcagctaat ttttgtattt ttagtagaga tggggtttta ccatgttggc 
               
               
                   
               
               
                 1501 
                 caggctggtc ttagactcct gacctcagtt gatccacctg cctcagcctc tgcattcagt 
               
               
                   
               
               
                 1561 
                 ttattcacat atttttggta actcccatgg cagctcctag gatttcagcg gtctgtgggc 
               
               
                   
               
               
                 1621 
                 cagaaagcag gcaccagggc tgacctcaag gccgtatcag agggccaagc agagttcttt 
               
               
                   
               
               
                 1681 
                 tggatacctg cttttcatcc cacagggcct tagagtcaga ggtaaggtag caacagagct 
               
               
                   
               
               
                 1741 
                 agaatggggc aatgcactct taccctcctt ctcaactttt atttaagctg tgctaaatgt 
               
               
                   
               
               
                 1801 
                 tttcttcaag ggaaccagat ttagttcttt acagaatttt ccagtgaaat aaaacatgtt 
               
               
                   
               
               
                 1861 
                 gtaatagctg tgtttgagat gaaataagag gttgtgggta gaggggaggc acctaaagga 
               
               
                   
               
               
                 1921 
                 aaagaggaaa ggtgcctggg ctacctatgc agataacctg gagtggactt cactgtggac 
               
               
                   
               
               
                 1981 
                 tcgtggtact aaggcttggc ctggacaggc agtctagggg gtatgggaat acacggtgtg 
               
               
                   
               
               
                 2041 
                 gttgttcaac tatttgcaaa ggtcaaccaa atagaccaca tgttcgcaaa gtatcatctg 
               
               
                   
               
               
                 2101 
                 aggaaattaa gtaccttctt agccctctca gtcataaatt tgaacaaatt ttaatacact 
               
               
                   
               
               
                 2161 
                 tccctcatgc ccttctatat aaaacttaat accattagtt ccccattctt gacattttat 
               
               
                   
               
               
                 2221 
                 ttcagttttt attatatatt tatttgaaat atttattaaa ttatctgacc tacagaacta 
               
               
                   
               
               
                 2281 
                 aaaaaaaaaa aaaaaaa 
               
               
                   
               
             
          
           
               
                 42: CK004097   EIF4EBP 
                   
               
             
          
           
               
                 1 
                 gggacatttc caagggtatt taaactctca ctctgccacc tttctaaggg tgggaggctg 
                   
               
               
                   
               
               
                 61 
                 gcagagatgc tgcaatgctt gataatcatt tggccacact gaaatttcca aagggagctc 
               
               
                   
               
               
                 121 
                 ttgccggtgc ttaaaaccaa aactcctgga cacttagaaa attccatgaa tctagcacaa 
               
               
                   
               
               
                 181 
                 aatatccatt cttgcccaag tgtatcccct ttctctccag cttaatcttt tttttttttt 
               
               
                   
               
               
                 241 
                 ttttttaaag cccaggccaa gggtactttt aactggaaac tggggaggag ggaagaacac 
               
               
                   
               
               
                 301 
                 tagcagggag ctaagaggca ggttgctggg taagccatcc tgctcctacc tggtgcctgt 
               
               
                   
               
               
                 361 
                 atctacattg ctgagtgctg tgcgccagtg cctttccttc atctgcagat ggagcccatc 
               
               
                   
               
               
                 421 
                 tctttccacc tgggtgagga gaccctctgc tactccaggg gtaaacctta aagaaggtgt 
               
               
                   
               
               
                 481 
                 cttgaagagc ccaaaggaca ctcacgtgct aaggtgtcca ttttatgcat ctttaaaata 
               
               
                   
               
               
                 541 
                 ttttatttaa aaaaaaaaat agccctgccc tgtcttagtg ccactaacgg cccagattca 
               
               
                   
               
               
                 601 
                 ttcattctga atggaaaaac ngagactgcc agcactttcc tttggtcctt ccn 
               
               
                   
               
             
          
           
               
                 43: NM_144683   MGC32380 
                   
               
             
          
           
               
                 1 
                 catggaggcg ctgctgctgg gcgcggggtt gctgctgggc gcttacgtgc ttgtctacta 
                   
               
               
                   
               
               
                 61 
                 caacctggtg aaggccccgc cgtgcggcgg catgggcaac ctgcggggcc gcacggccgt 
               
               
                   
               
               
                 121 
                 ggtcacgggt gagtgcggag gcgggtgagt gcgagctggc ggggcgcgcg gagaggaggc 
               
               
                   
               
               
                 181 
                 cgggccggcg gtagcagcgg cccgccgggc tcagctcagc tcggctcccg cccgcggtcc 
               
               
                   
               
               
                 241 
                 gcaggcgcca acagcggcat cggaaagatg acggcgctgg agctggcgcg ccggggagcg 
               
               
                   
               
               
                 301 
                 cgcgtggtgc tggcctgccg cagccaggag cgcggggagg cggctgcctt cgacctccgc 
               
               
                   
               
               
                 361 
                 caggagagtg ggaacaatga ggtcatcttc atggccttgg acttggccag tctggcctcg 
               
               
                   
               
               
                 421 
                 gtgcgggcct ttgccactgc ctttctgagc tctgagccac ggttggacat cctcatccac 
               
               
                   
               
               
                 481 
                 aatgccggta tcagttcctg tggccggacc cgtgaggcgt ttaacctgct gcttcgggtg 
               
               
                   
               
               
                 541 
                 aaccatatcg gtccctttct gctgacacat ctgctgctgc cttgcctgaa ggcatgtgcc 
               
               
                   
               
               
                 601 
                 cctagccgcg tggtggtggt agcctcagct gcccactgtc ggggacgtct tgacttcaaa 
               
               
                   
               
               
                 661 
                 cgcctggacc gcccagtggt gggctggcgg caggagctgc gggcatatgc tgacactaag 
               
               
                   
               
               
                 721 
                 ctggctaatg tactgtttgc ccgggagctc gccaaccagc ttgaggccac tggcgtcacc 
               
               
                   
               
               
                 781 
                 tgctatgcag cccacccagg gcctgtgaac tcggagctgt tcctgcgcca tgttcctgga 
               
               
                   
               
               
                 841 
                 tggctgcgcc cacttttgcg cccattggct tggctggtgc tccgggcacc aagagggggt 
               
               
                   
               
               
                 901 
                 gcccagacac ccctgtattg tgctctacaa gagggcatcg agcccctcag tgggagatat 
               
               
                   
               
               
                 961 
                 tttgccaact gccatgtgga agaggtgcct ccagctgccc gagacgaccg ggcagcccat 
               
               
                   
               
               
                 1021 
                 cggctatggg aggccagcaa gaggctggca gggcttgggc ctggggagga tgctgaaccc 
               
               
                   
               
               
                 1081 
                 gatgaagacc cccagtctga ggactcagag gccccatctt ctctaagcac cccccaccct 
               
               
                   
               
               
                 1141 
                 gaggagccca cagtttctca accttacccc agccctcaga gctcaccaga tttgtctaag 
               
               
                   
               
               
                 1201 
                 atgacgcacc gaattcaggc taaagttgag cctgagatcc agctctccta accctcaggc 
               
               
                   
               
               
                 1261 
                 caggatgctt gccatggcac ttcatggtcc ttgaaaacct cggatgtgtg cgaggccatg 
               
               
                   
               
               
                 1321 
                 ccctggacac tgacgggttt gtgatcttga cctccgtggt tactttctgg ggccccaagc 
               
               
                   
               
               
                 1381 
                 tgtgccctgg acatctcttt tcctggttga aggaataatg ggtgattatt tcttcctgag 
               
               
                   
               
               
                 1441 
                 agtgacagta accccagatg gagagatagg ggtatgctag acactgtgct tctcggaaat 
               
               
                   
               
               
                 1501 
                 ttggatgtag tattttcagg ccccaccctt attgattctg atcagctctg gagcagaggc 
               
               
                   
               
               
                 1561 
                 agggagtttg caatgtgatg cactgccaac attgagaatt agtgaactga tccctttgca 
               
               
                   
               
               
                 1621 
                 accgtctagc taggtagtta aattaccccc atgttaatga agcggaatta ggctcccgag 
               
               
                   
               
               
                 1681 
                 ctaagggact cgcctagggt ctcacagtga gtaggaggag ggcctgggat ctgaacccaa 
               
               
                   
               
               
                 1741 
                 gggtctgagg ccagggccga ctgccgtaag atgggtgctg agaagtgagt cagggcaggg 
               
               
                   
               
               
                 1801 
                 cagctggtat cgaggtgccc catgggagta aggggacgcc ttccgggcgg atgcagggct 
               
               
                   
               
               
                 1861 
                 ggggtcatct gtatctgaag cccctcggaa taaagcgcgt tgaccgccaa aaaaaaaaaa 
               
               
                   
               
               
                 1921 
                 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 
               
               
                   
               
             
          
           
               
                 44: NM_004600   SSA2 
                   
               
             
          
           
               
                 1 
                 tcctgcttgt cggcatcgct ccccacaggc cgacgtcgag agggcctgct ttactcctcc 
                   
               
               
                   
               
               
                 61 
                 tctttctcct ccttctcccg cggcttctgc gcggagaggc gtcgcccggg atctgggttt 
               
               
                   
               
               
                 121 
                 tggaagaagg atctttgtgg gaagacaggg tgaatttatc acagaggaat aacgagggag 
               
               
                   
               
               
                 181 
                 aggagaaagg tttcctaaag acaaaaaaaa aaatggagga atctgtaaac caaatgcagc 
               
               
                   
               
               
                 241 
                 cactgaatga gaagcagata gccaattctc aggatggata tgtatggcaa gtcactgaca 
               
               
                   
               
               
                 301 
                 tgaatcgact acaccggttc ttatgtttcg gttctgaagg tgggacttat tatatcaaag 
               
               
                   
               
               
                 361 
                 aacagaagtt gggccttgaa aatgctgaag ctttaattag attgattgaa gatggcagag 
               
               
                   
               
               
                 421 
                 gatgtgaagt gatacaagaa ataaagtcat ttagtcaaga aggcagaacc acaaagcaag 
               
               
                   
               
               
                 481 
                 agcctatgct ctttgcactt gccatttgtt cccagtgctc cgacataagc acaaaacaag 
               
               
                   
               
               
                 541 
                 cagcatttaa agctgtttct gaagtttgtc gcattcctac ccatctcttt acttttatcc 
               
               
                   
               
               
                 601 
                 agtttaagaa agatctgaag gaaagcatga aatgtggcat gtggggtcgt gccctccgga 
               
               
                   
               
               
                 661 
                 aggctatagc ggactggtac aatgagaaag gtggcatggc ccttgctctg gcagttacaa 
               
               
                   
               
               
                 721 
                 aatataaaca gagaaatggc tggtctcaca aagatctatt aagattgtca catcttaaac 
               
               
                   
               
               
                 781 
                 cttccagtga aggacttgca attgtgacca aatatattac aaagggctgg aaagaagttc 
               
               
                   
               
               
                 841 
                 atgaattgta taaagaaaaa gcactctctg tggagactga aaaattatta aagtatctgg 
               
               
                   
               
               
                 901 
                 aggctgtaga gaaagtgaag cgcacaagag atgagctaga agtcattcat ctaatagaag 
               
               
                   
               
               
                 961 
                 aacatagatt agttagagaa catcttttaa caaatcactt aaagtctaaa gaggtatgga 
               
               
                   
               
               
                 1021 
                 aggctttgtt acaagaaatg ccgcttactg cattactaag gaatctagga aagatgactg 
               
               
                   
               
               
                 1081 
                 ctaattcagt acttgaacca ggaaattcag aagtatcttt agtatgtgaa aaactgtgta 
               
               
                   
               
               
                 1141 
                 atgaaaaact attaaaaaag gctcgtatac atccatttca tattttgatc gcattagaaa 
               
               
                   
               
               
                 1201 
                 cttacaagac aggtcatggt ctcagaggga aactgaagtg gcgccctgat gaagaaattt 
               
               
                   
               
               
                 1261 
                 tgaaagcatt ggatgctgct ttttataaaa catttaagac agttgaacca actggaaaac 
               
               
                   
               
               
                 1321 
                 gtttcttact agctgttgat gtcagtgctt ctatgaacca aagagttttg ggtagtatac 
               
               
                   
               
               
                 1381 
                 tcaacgctag tacagttgct gcagcaatgt gcatggttgt cacacgaaca gaaaaagatt 
               
               
                   
               
               
                 1441 
                 cttatgtagt tgctttttcc gatgaaatgg taccatgtcc agtgactaca gatatgacct 
               
               
                   
               
               
                 1501 
                 tacaacaggt tttaatggct atgagtcaga tcccagcagg tggaactgat tgctctcttc 
               
               
                   
               
               
                 1561 
                 caatgatctg ggctcagaag acaaacacac ctgctgatgt cttcattgta ttcactgata 
               
               
                   
               
               
                 1621 
                 atgagacctt tgctggaggt gtccatcctg ctattgctct gagggagtat cgaaagaaaa 
               
               
                   
               
               
                 1681 
                 tggatattcc agctaaattg attgtttgtg gaatgacatc aaatggtttc accattgcag 
               
               
                   
               
               
                 1741 
                 acccagatga tagaggcatg ttggatatgt gcggctttga tactggagct ctggatgtaa 
               
               
                   
               
               
                 1801 
                 ttcgaaattt cacattagat atgatttaac cataagcagc agcacgatcc agagatccat 
               
               
                   
               
               
                 1861 
                 tgccatcagt gatctcacta aaaatataca gctacttccc agctaatctc cacccaatga 
               
               
                   
               
               
                 1921 
                 atgatgatgg tatagtatgt gcataatgga aagttacctt actgaaaaaa aaaaaagaag 
               
               
                   
               
               
                 1981 
                 gaaaaataag atgggcccaa aggtctatct actaaactag ctcttgggga aatagcttca 
               
               
                   
               
               
                 2041 
                 ggatactgta gtttcctcta tctaatagag aactttttgt taacagacac tgtaaaatag 
               
               
                   
               
               
                 2101 
                 ttttgctttg ttgaataata catgtgtacc taaaagaggt aagagcaaaa agtgtaattc 
               
               
                   
               
               
                 2161 
                 cacatcatgt tacttgagaa gtgcttaacg ttttcttaaa tgttttcatt gggaaaggac 
               
               
                   
               
               
                 2221 
                 agctttgata atgtccaaat actctgaaat gcactagacc atataactgt gatgaaatat 
               
               
                   
               
               
                 2281 
                 gaaactcatc tgtaaacttt tataccaagg gggtaaaaaa aaaaactaag gcatttgatt 
               
               
                   
               
               
                 2341 
                 aaattatgaa tgagttttac aaattccttt cagagtttta ctaagatcac acaaataaca 
               
               
                   
               
               
                 2401 
                 gctttcttat tcagtgaaaa agatatttta tttctgatgt tttatttgca ctcgtggaat 
               
               
                   
               
               
                 2461 
                 atgttaccat taatcagaaa catcatggca acccctaaga atagactaag tttgtgttgg 
               
               
                   
               
               
                 2521 
                 ctgagggatt ctatttggtt tgcttttttt tttttgcttt gttatatttt attgctacaa 
               
               
                   
               
               
                 2581 
                 ggggtgtgac ttgataatga tttcctctga attataataa catagccaga tgtagtctca 
               
               
                   
               
               
                 2641 
                 cactgttttt catactctta agtgtaaata atataaaatg tttcaagcgc ttaactcccc 
               
               
                   
               
               
                 2701 
                 ctcattcaca aagtataaca attaaaatct caactataac cagtttagct ttttccttac 
               
               
                   
               
               
                 2761 
                 ttttaaaata aaatttttta cttttaacta tttttttagt taatattttt aaaagtatac 
               
               
                   
               
               
                 2821 
                 atgtcaatgg cctctttgtc cattattcat tttgtggcaa aatattcttc tttgatagtg 
               
               
                   
               
               
                 2881 
                 taaacaaata ataaagcaat ctaggtcctt taggtttgaa aggcaatttt tgagtagcat 
               
               
                   
               
               
                 2941 
                 attaccagct agccagtcac taggaatttt tttcagtatt atttgtatgt attaaacttt 
               
               
                   
               
               
                 3001 
                 tcattacact aaagtgcatt attttattga gcaagtatcc ttcattgtga ggtttgacat 
               
               
                   
               
               
                 3061 
                 taaagcaatc tgttgaaatg ccaaaaaaaa aaaaaaaa 
               
               
                   
               
             
          
           
               
                 45: NM_002730   PRKACA 
                   
               
             
          
           
               
                 1 
                 gatcttgggc tgaggttccc gggcgggcgg gcgcggagag acgcgggaag caggggctgg 
                   
               
               
                   
               
               
                 61 
                 gcgggggtcg cggcgccgca gctagcgcag ccagcccgag ggccgccgcc gccgccgccc 
               
               
                   
               
               
                 121 
                 agcgcgctcc ggggccgccg gccgcagcca gcacccgccg cgccgcagct ccgggaccgg 
               
               
                   
               
               
                 181 
                 ccccggccgc cgccgccgcg atgggcaacg ccgccgccgc caagaagggc agcgagcagg 
               
               
                   
               
               
                 241 
                 agagcgtgaa agaattctta gccaaagcca aagaagattt tcttaaaaaa tgggaaagtc 
               
               
                   
               
               
                 301 
                 ccgctcagaa cacagcccac ttggatcagt ttgaacgaat caagaccctc ggcacgggct 
               
               
                   
               
               
                 361 
                 ccttcgggcg ggtgatgctg gtgaaacaca aggagaccgg gaaccactat gccatgaaga 
               
               
                   
               
               
                 421 
                 tcctcgacaa acagaaggtg gtgaaactga aacagatcga acacaccctg aatgaaaagc 
               
               
                   
               
               
                 481 
                 gcatcctgca agctgtcaac tttccgttcc tcgtcaaact cgagttctcc ttcaaggaca 
               
               
                   
               
               
                 541 
                 actcaaactt atacatggtc atggagtacg tgcccggcgg ggagatgttc tcacacctac 
               
               
                   
               
               
                 601 
                 ggcggatcgg aaggttcagt gagccccatg cccgtttcta cgcggcccag atcgtcctga 
               
               
                   
               
               
                 661 
                 cctttgagta tctgcactcg ctggatctca tctacaggga cctgaagccg gagaatctgc 
               
               
                   
               
               
                 721 
                 tcattgacca gcagggctac attcaggtga cagacttcgg tttcgccaag cgcgtgaagg 
               
               
                   
               
               
                 781 
                 gccgcacttg gaccttgtgc ggcacccctg agtacctggc ccctgagatt atcctgagca 
               
               
                   
               
               
                 841 
                 aaggctacaa caaggccgtg gactggtggg ccctgggggt tcttatctat gaaatggccg 
               
               
                   
               
               
                 901 
                 ctggctaccc gcccttcttc gcagaccagc ccatccagat ctatgagaag atcgtctctg 
               
               
                   
               
               
                 961 
                 ggaaggtgcg cttcccttcc cacttcagct ctgacttgaa ggacctgctg cggaacctcc 
               
               
                   
               
               
                 1021 
                 tgcaggtaga tctcaccaag cgctttggga acctcaagaa tggggtcaac gatatcaaga 
               
               
                   
               
               
                 1081 
                 accacaagtg gtttgccaca actgactgga ttgccatcta ccagaggaag gtggaagctc 
               
               
                   
               
               
                 1141 
                 ccttcatacc aaagtttaaa ggccctgggg atacgagtaa ctttgacgac tatgaggaag 
               
               
                   
               
               
                 1201 
                 aagaaatccg ggtctccatc aatgagaagt gtggcaagga gttttctgag ttttaggggc 
               
               
                   
               
               
                 1261 
                 atgcctgtgc ccccatgggt tttctttttt cttttttctt ttttttggtc gggggggtgg 
               
               
                   
               
               
                 1321 
                 gagggttgga ttgaacagcc agagggcccc agagttcctt gcatctaatt tcacccccac 
               
               
                   
               
               
                 1381 
                 cccaccctcc agggttaggg ggagcaggaa gcccagataa tcagagggac agaaacacca 
               
               
                   
               
               
                 1441 
                 gctgctcccc ctcatcccct tcaccctcct gccccctctc ccacttttcc cttcctcttt 
               
               
                   
               
               
                 1501 
                 ccccacagcc ccccagcccc tcagccctcc cagcccactt ctgcctgttt taaacgagtt 
               
               
                   
               
               
                 1561 
                 tctcaactcc agtcagacca ggtcttgctg gtgtatccag ggacagggta tggaaagagg 
               
               
                   
               
               
                 1621 
                 ggctcacgct taactccagc ccccacccac acccccatcc cacccaacca caggccccac 
               
               
                   
               
               
                 1681 
                 ttgctaaggg caaatgaacg aagcgccaac cttcctttcg gagtaatcct gcctgggaag 
               
               
                   
               
               
                 1741 
                 gagagatttt tagtgacatg ttcagtgggt tgcttgctag aattttttta aaaaaacaac 
               
               
                   
               
               
                 1801 
                 aatttaaaat cttatttaag ttccaccagt gcctccctcc ctccttcctc tactcccacc 
               
               
                   
               
               
                 1861 
                 cctcccatgt ccccccattc ctcaaatcca ttttaaagag aagcagactg actttggaaa 
               
               
                   
               
               
                 1921 
                 gggaggcgct ggggtttgaa cctccccgct gctaatctcc cctgggcccc tccccgggga 
               
               
                   
               
               
                 1981 
                 atcctctctg ccaatcctgc gagggtctag gcccctttag gaagcctccg ctctcttttt 
               
               
                   
               
               
                 2041 
                 ccccaacaga cctgtcttca cccttgggct ttgaaagcca gacaaagcag ctgcccctct 
               
               
                   
               
               
                 2101 
                 ccctgccaaa gaggagtcat cccccaaaaa gacagagggg gagccccaag cccaagtctt 
               
               
                   
               
               
                 2161 
                 tcctcccagc agcgtttccc cccaactcct taattttatt ctccgctaga ttttaacgtc 
               
               
                   
               
               
                 2221 
                 cagccttccc tcagctgagt ggggagggca tccctgcaaa agggaacaga agaggccaag 
               
               
                   
               
               
                 2281 
                 tccccccaag ccacggcccg gggttcaagg ctagagctgc tggggagggg ctgcctgttt 
               
               
                   
               
               
                 2341 
                 tactcaccca ccagcttccg cctcccccat cctgggcgcc cctcctccag cttagctgtc 
               
               
                   
               
               
                 2401 
                 agctgtccat cacctctccc ccactttctc atttgtgctt ttttctctcg taatagaaaa 
               
               
                   
               
               
                 2461 
                 gtggggagcc gctggggagc caccccattc atccccgtat ttccccctct cataacttct 
               
               
                   
               
               
                 2521 
                 ccccatccca ggaggagttc tcaggcctgg ggtggggccc cgggtgggtg cgggggcgat 
               
               
                   
               
               
                 2581 
                 tcaacctgtg tgctgcgaag gacgagactt cctcttgaac agtgtgctgt tgtaaacata 
               
               
                   
               
               
                 2641 
                 tttgaaaact attaccaata aagttttgtt taaaaaaaaa aaaaaaaaa 
               
               
                   
               
             
          
           
               
                 46: NM_005102   FEZ2 
                   
               
             
          
           
               
                 1 
                 ccggagcctc ctggaccagg agaactgtaa cgcgagcccc gagccatggg cgaaaggcgg 
                   
               
               
                   
               
               
                 61 
                 ggccgagacg ggttgggggc gccgacggtt tcccggccct ggctgcagct tggaggagaa 
               
               
                   
               
               
                 121 
                 gctgagcctg tgcttccgcc cctcggatcc gggcgccgag cccgaggacg gccgtgcggc 
               
               
                   
               
               
                 181 
                 catcacggag ctcaactcct gcagggggac gagatttgga atgccctgac agataattat 
               
               
                   
               
               
                 241 
                 gggaatgtga tgcctgtaga ctggaagtca tcgcatacta ggaccttgca cttgcttact 
               
               
                   
               
               
                 301 
                 ctgaacctct cagaaaaagg ggtaagtgac agtttgctct ttgatacatc agatgatgaa 
               
               
                   
               
               
                 361 
                 gagctgagag aacagctgga tatgcactca atcatcgtct cctgtgttaa tgatgaaccc 
               
               
                   
               
               
                 421 
                 ctcttcacgg cagaccaggt tattgaagaa attgaagaaa tgatgcagga atcaccggac 
               
               
                   
               
               
                 481 
                 ccagaagatg atgaaacccc tacacagtca gatcggcttt caatgctttc ccaggaaatt 
               
               
                   
               
               
                 541 
                 caaactctca agaggtctag taccggcagt tatgaagaga gagtgaaaag gctctcagtg 
               
               
                   
               
               
                 601 
                 tctgagttaa atgaaatcct ggaagaaatt gagactgcca ttaaggagta ctctgaggag 
               
               
                   
               
               
                 661 
                 ctggtgcagc agttggcttt acgagatgaa ctggagtttg aaaaggaagt gaaaaacagc 
               
               
                   
               
               
                 721 
                 tttatttctg ttcttattga agtgcaaaac aaacagaaag agcacaaaga aacagcaaaa 
               
               
                   
               
               
                 781 
                 aagaaaaaga aactaaaaaa tggcagctct cagaatggga agaatgagag aagtcatatg 
               
               
                   
               
               
                 841 
                 cccggcacat atttgactac agtcattcct tatgagaaaa aaaacggacc accgtctgtt 
               
               
                   
               
               
                 901 
                 gaagatcttc aaatattaac aaaaattctt cgtgccatga aggaggacag tgaaaaagtt 
               
               
                   
               
               
                 961 
                 ccgagcttgt taactgatta tattctgaaa gttctgtgtc ctacatagag cagcaacttt 
               
               
                   
               
               
                 1021 
                 atctgcggtg ggctccaagc tagatttccg acagcattat tctgagagct ggctaccatt 
               
               
                   
               
               
                 1081 
                 acccttcttg ctattggaaa ctcagcacat ttgaacttgg gtttgattca gtattaacag 
               
               
                   
               
               
                 1141 
                 atcttgacta cactaattct ttatattata gaaccaacgg aaatatgggc actattttga 
               
               
                   
               
               
                 1201 
                 attctagaga tggtttttgt taaatctact aataaactgt tctcttagta gattaagaga 
               
               
                   
               
               
                 1261 
                 gagtaatatt aattgtgcat gtgcagttgt atttctcatt aactgacagt atgcccattt 
               
               
                   
               
               
                 1321 
                 gtttttatgg ctttcttatc taaactgcac tgatgaacta gattaaagcc ttgggagatt 
               
               
                   
               
               
                 1381 
                 tatactataa attcagtgat ggcaagaacc aacactgttt ttttgtgaga attgtcagtg 
               
               
                   
               
               
                 1441 
                 taactattac ctaccagtat tgttcagaga gattgaaaca gaataaacgg gctgttcttg 
               
               
                   
               
               
                 1501 
                 aagaagcaaa accagaatat gcattacttt ggtttaatac ttagtgctaa cattgaaact 
               
               
                   
               
               
                 1561 
                 gttggtggtg atggattttg tagcttgctg cttgtttcac cactggtcaa attttaacca 
               
               
                   
               
               
                 1621 
                 ttaaattgcc attcactttt agaatcttgt atttaagtaa gttttgattt tcaaatgttc 
               
               
                   
               
               
                 1681 
                 tgcttcatgt gtctgtgaag aattgtactt ttttaaaagt gtgtgtcctc tgaggtgctt 
               
               
                   
               
               
                 1741 
                 gagaaagtgt acactgcaga actgcccatt ctcattactg tgtcctattt tattcatgcc 
               
               
                   
               
               
                 1801 
                 tgtgtgtttt tcttaagtat gaattctaga tacagctact tatggattca tcaatatcat 
               
               
                   
               
               
                 1861 
                 gagcactttt gctggttcca gtcaaatcaa tggcatttaa taaatttttt aagaagtaaa 
               
               
                   
               
               
                 1921 
                 aaaaaaaaaa aaaaaa 
               
               
                   
               
             
          
           
               
                 47: NM_005839   SRRM1 
                   
               
             
          
           
               
                 1 
                 ggagtttagg gcctgacaga agcccgcccc cgctggcgct cgtgcgcacg cgtggcgggc 
                   
               
               
                   
               
               
                 61 
                 tctcggcgca ctgagcaggc gcggcctcgt gtcggccgga gggggcgggc gcaacgacgc 
               
               
                   
               
               
                 121 
                 gcgctgcgtc ccggcgctcg gctttccctc cgccggtccc gccctccgtc gcggcggcgc 
               
               
                   
               
               
                 181 
                 ggtgtaccct gggataggga gcgatctccg agcgaggcgg caagatggac gcgggatttt 
               
               
                   
               
               
                 241 
                 tccgcggaac aagtgcagaa caggataatc ggttcagcaa caaacagaag aaactactga 
               
               
                   
               
               
                 301 
                 agcagctgaa atttgcagaa tgcctagaaa aaaaggtgga catgagcaaa gtaaatttgg 
               
               
                   
               
               
                 361 
                 aggttataaa gccttggata acaaaaagag taacggaaat ccttgggttt gaagatgatg 
               
               
                   
               
               
                 421 
                 ttgtgattga gtttatattc aaccagctgg aagtgaagaa tccagactcc aaaatgatgc 
               
               
                   
               
               
                 481 
                 aaatcaacct gactggattt ttgaatggaa aaaatgctcg agaatttatg ggagaactgt 
               
               
                   
               
               
                 541 
                 ggcccctgct gctaagtgca caagaaaaca tcgcgggaat cccttctgct ttcctagaac 
               
               
                   
               
               
                 601 
                 tgaagaaaga agaaataaaa caaagacaga ttgaacaaga aaaactggca tctatgaaaa 
               
               
                   
               
               
                 661 
                 agcaagatga agacaaagat aaaagagata aggaagaaaa agaaagcagc agagaaaaaa 
               
               
                   
               
               
                 721 
                 gggagcggtc tcgtagccca agaagacgca aatccagatc tccttcccct agaagacgat 
               
               
                   
               
               
                 781 
                 cttcccctgt caggagagag agaaagcgca gtcattctcg atctccccgt cacagaacca 
               
               
                   
               
               
                 841 
                 agagccggag tccttcccct gctccagaaa agaaggaaaa aactccagag ctcccagaac 
               
               
                   
               
               
                 901 
                 cttcagtgaa agtaaaagaa ccttcagtac aagaggctac ttctactagt gacattctga 
               
               
                   
               
               
                 961 
                 aagttcccaa acctgaacct ataccagagc ctaaagaacc ttctccggaa aaaaattcca 
               
               
                   
               
               
                 1021 
                 aaaaagaaaa ggagaaggag aagacccgac cacgatctcg gtcacgctcc aaatcaagat 
               
               
                   
               
               
                 1081 
                 cccggacgcg gtcccgctct ccttctcaca ctcgacctag acggcgccat agatcccgat 
               
               
                   
               
               
                 1141 
                 caagatcgta ttcacctaga aggcggccaa gcccaagaag gcggccatct cctcgaagaa 
               
               
                   
               
               
                 1201 
                 gaactccgcc aagaagaatg cctcctccac caaggcatag aaggagtaga tctccagtaa 
               
               
                   
               
               
                 1261 
                 gacgaagaag acgttcgtca gcatccttgt ctgggagtag ctcatcatcc tcttcatctc 
               
               
                   
               
               
                 1321 
                 gttcacggtc accaccaaag aagcctccca agaggacatc cagcccccct cggaaaactc 
               
               
                   
               
               
                 1381 
                 gtaggttatc tccttcagca agtcctccaa ggcgaaggca caggccatca cctcctgcaa 
               
               
                   
               
               
                 1441 
                 ctccaccacc caaaactcgg cattccccta caccccagca gtcaaaccgt acaagaaaaa 
               
               
                   
               
               
                 1501 
                 gtcgtgtttc tgtgtctcca gggagaactt caggtaaagt gacaaaacat aaaggtactg 
               
               
                   
               
               
                 1561 
                 agaaaagaga atccccttca ccagcaccga agcctagaaa agtagagtta tctgaatcgg 
               
               
                   
               
               
                 1621 
                 aagaagataa aggtggcaaa atggctgcag cagattctgt gcagcagaga cgccaataca 
               
               
                   
               
               
                 1681 
                 gacgacaaaa ccagcagtct tcatctgact ctggctcctc ctcctcctca gaagatgaac 
               
               
                   
               
               
                 1741 
                 gacccaagag atcccatgtg aagaatggtg aggttggcag gcggcggaga cattcccctt 
               
               
                   
               
               
                 1801 
                 cccggagtgc ttctccatca ccacgaaagc gccaaaaaga gacttcccct cgtggtagac 
               
               
                   
               
               
                 1861 
                 ggaggagaag tccatcccca ccacccacca gaaggcgacg gtctccttct cccgcccctc 
               
               
                   
               
               
                 1921 
                 ctcctcgacg gcgcaggact cccacaccac caccacgacg aaggactcct tctcctcccc 
               
               
                   
               
               
                 1981 
                 cacgtcggcg ctcaccttct cctagaagat actctcctcc aatacagagg agatactctc 
               
               
                   
               
               
                 2041 
                 cttctccacc tccaaagaga agaacggctt cacctcctcc ccctcctaaa cgaagagcat 
               
               
                   
               
               
                 2101 
                 caccatctcc accaccaaag cggcgggtct cccattctcc acctcccaaa caaagaagct 
               
               
                   
               
               
                 2161 
                 ccccagtcac caagagacgt tcaccttcat tatcatccaa gcataggaaa gggtcttccc 
               
               
                   
               
               
                 2221 
                 caagccgctc tacccgggag gcccgatcac cacaaccaaa caaacggcat tcgccctcac 
               
               
                   
               
               
                 2281 
                 cacggcctcg agctcctcag acctcctcaa gtcctccacc cgttcgaaga ggagcgtcgt 
               
               
                   
               
               
                 2341 
                 catcacccca aagaaggcag tccccgtctc caagtactag gcccattagg agagtctcca 
               
               
                   
               
               
                 2401 
                 ggactccgga acctaaaaag ataaaaaagg ctgcttcccc aagcccacag tctgtaagaa 
               
               
                   
               
               
                 2461 
                 gggtctcatc ctcccgatct gtctccgggt ctcctgagcc agcagctaaa aagcccccag 
               
               
                   
               
               
                 2521 
                 cacctccatc ccccgtccag tctcagtcac cgtctacaaa ctggtcacca gctgtaccgg 
               
               
                   
               
               
                 2581 
                 tcaaaaaggc caaaagccca acaccgagcc catcaccgcc aagaaattca gatcaggaag 
               
               
                   
               
               
                 2641 
                 gaggtggaaa gaaaaagaag aaaaagaagg acaagaaaca caaaaaggat aagaagcaca 
               
               
                   
               
               
                 2701 
                 agaagcacaa aaaacacaag aaggaaaagg ctgtggctgc agctgctgca gctgctgtga 
               
               
                   
               
               
                 2761 
                 cccctgcagc cattgcagct gccacaacca cattagcaca ggaagagcca gtggcagcgc 
               
               
                   
               
               
                 2821 
                 cagagccgaa gaaggagact gaaagtgaag ctgaagataa ccttgatgat ttagaaaagc 
               
               
                   
               
               
                 2881 
                 acctgcgtga aaaggccctg agatcaatga ggaaggccca agtgtcccca cagtcttagg 
               
               
                   
               
               
                 2941 
                 gggaaatgtt tgttatgatg taaattttat ttggtttgta cgcagttcaa tttcaaaatt 
               
               
                   
               
               
                 3001 
                 gctaaaatgt gtttgagctt tagactataa catttgttgt aataattgct aggttgaagt 
               
               
                   
               
               
                 3061 
                 tcaacatgta aaaaaagggg gcatggattt acattgcaaa aggtgtccac agtgtattag 
               
               
                   
               
               
                 3121 
                 tgacattctt tcattgacag ctgacataat tcattgagtg aaatatttta agccaaaaaa 
               
               
                   
               
               
                 3181 
                 aaattccctt tttaaaaaag ggggtttaaa tactgttggc atttttatgg ttcctttaaa 
               
               
                   
               
               
                 3241 
                 tgccctagct attcccagag gggttttttt gtttgttttt ttggttttga ttttcttttt 
               
               
                   
               
               
                 3301 
                 gtttttcttt cttcttctta tttttttcat ttgagtctta gctcccattt aagttatgct 
               
               
                   
               
               
                 3361 
                 tctgaccttg tatggtctgt aagcttgccc agaaataaga ccactgtttt gaactaccac 
               
               
                   
               
               
                 3421 
                 aaaagtataa atgaatattt taatgccaca atctttcctg ttgcctgtgg agtctctgct 
               
               
                   
               
               
                 3481 
                 gaaatgaatc aggattcgag ctctaggatg agacagaaaa tgaaagcatg ttgtttgcca 
               
               
                   
               
               
                 3541 
                 ggacactgtg ggtttatatt gatgtgtaac aagttgattt ggaacactgg actctcattc 
               
               
                   
               
               
                 3601 
                 tgttattctg gttttgtttt ttttgttttg ttttttttct tttgtaaagg caatgagcta 
               
               
                   
               
               
                 3661 
                 gtcccagaaa ggatccttca gttacataca atttgtttaa tgaaatgtca tggctctgtt 
               
               
                   
               
               
                 3721 
                 catatttttg tcttgttctt ccaattggta tatacaactt tcagagcctc ttgtatttgg 
               
               
                   
               
               
                 3781 
                 aaggctggaa gggcccagac tttggaatag tgtcttggtt tcactgtttt tgttttgatt 
               
               
                   
               
               
                 3841 
                 ttttttttgt tttgattttt tttaaactaa agctatataa agcttgtgga ttaaacagaa 
               
               
                   
               
               
                 3901 
                 taaatttcta aatttaaaaa tttaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
               
               
                   
               
               
                 3961 
                 aaaaaaaaaa aaggaaaaaa aaaaaaaaaa 
               
               
                   
               
             
          
           
               
                 48: NM_006207   PDGFRL 
                   
               
             
          
           
               
                 1 
                 cctgcgtccc cgccccgcgc agccgccgcg ctcctgcgct ccgaggtccg aggttcccga 
                   
               
               
                   
               
               
                 61 
                 gatgaaggtc tggctgctgc ttggtcttct gctggtgcac gaagcgctgg aggatgttac 
               
               
                   
               
               
                 121 
                 tggccaacac cttcccaaga acaagcgtcc aaaagaacca ggagagaata gaatcaaacc 
               
               
                   
               
               
                 181 
                 taccaacaag aaggtgaagc ccaaaattcc taaaatgaag gacagggact cagccaattc 
               
               
                   
               
               
                 241 
                 agcaccaaag acgcagtcta tcatgatgca agtgctggat aaaggtcgct tccagaaacc 
               
               
                   
               
               
                 301 
                 cgccgctacc ctgagtctgc tggcggggca aactgtagag cttcgatgta aagggagtag 
               
               
                   
               
               
                 361 
                 aattgggtgg agctaccctg cgtatctgga cacctttaag gattctcgcc tcagcgtcaa 
               
               
                   
               
               
                 421 
                 gcagaatgag cgctacggcc agttgactct ggtcaactcc acctcggcag acacaggtga 
               
               
                   
               
               
                 481 
                 attcagctgc tgggtgcagc tctgcagcgg ctacatctgc aggaaggacg aggccaaaac 
               
               
                   
               
               
                 541 
                 gggctccacc tacatctttt ttacagagaa aggagaactc tttgtacctt ctcccagcta 
               
               
                   
               
               
                 601 
                 cttcgatgtt gtctacttga acccggacag acaggctgtg gttccttgtc gggtgaccgt 
               
               
                   
               
               
                 661 
                 gctgtcggcc aaagtcacgc tccacaggga attcccagcc aaggagatcc cagccaatgg 
               
               
                   
               
               
                 721 
                 aacggacatt gtttatgaca tgaagcgggg ctttgtgtat ctgcaacctc attccgagca 
               
               
                   
               
               
                 781 
                 ccagggtgtg gtttactgca gggcggaggc cgggggcaga tctcagatct ccgtcaagta 
               
               
                   
               
               
                 841 
                 ccagctgctc tacgtggcgg ttcccagtgg ccctccctca acaaccatct tggcttcttc 
               
               
                   
               
               
                 901 
                 aaacaaagtg aaaagtgggg acgacatcag tgtgctctgc actgtcctgg gggagcccga 
               
               
                   
               
               
                 961 
                 tgtggaggtg gagttcacct ggatcttccc agggcagaag gatgaaaggc ctgtgacgat 
               
               
                   
               
               
                 1021 
                 ccaagacact tggaggttga tccacagagg actgggacac accacgagaa tctcccagag 
               
               
                   
               
               
                 1081 
                 tgtcattaca gtggaagact tcgagacgat tgatgcagga tattacattt gcactgctca 
               
               
                   
               
               
                 1141 
                 gaatcttcaa ggacagacca cagtagctac cactgttgag ttttcctgac ttggaaaagg 
               
               
                   
               
               
                 1201 
                 aaatgtaatg aacttatgga aagcccattt gtgtacacag tcagctttgg ggttcctttt 
               
               
                   
               
               
                 1261 
                 attagtgctt tgccagaggc tgatgtcaag caccacaccc caaccccagc gtctcgtgag 
               
               
                   
               
               
                 1321 
                 tccgacccag acatccaaac taaaaggaag tcatccagtc tattcacaga agtgttaact 
               
               
                   
               
               
                 1381 
                 tttctaacag aaagcatgat tttgattgct tacctacata cgtgttccta gtttttatac 
               
               
                   
               
               
                 1441 
                 atgtgtaaac aattttatat aatcaatcat ttctattaaa tgagcacgtt tttgtaaaaa 
               
               
                   
               
               
                 1501 
                 at 
               
               
                   
               
             
          
           
               
                 49: AI096936   SNX13 
                   
               
             
          
           
               
                 1 
                 aaaaaaatta aggctaacca agtgcatcca ttgttcaatg gcacaattga tttcagcaac 
                   
               
               
                   
               
               
                 61 
                 tatttggaat atcctaatta taggaaatgc ccatctaagt gatatattta aataatacaa 
               
               
                   
               
               
                 121 
                 tcaatttttt aaggtgaata aactatgatg gtttctaaat agtgtacatg ttacctgaaa 
               
               
                   
               
               
                 181 
                 aatcagaaaa cacaaagaat gattaatttc gaaagttctt gcctaaaggc accactgact 
               
               
                   
               
               
                 241 
                 taaaaaacat tcaaaatcaa ataccacaag acataaagcc tcttcatgta tatattcata 
               
               
                   
               
               
                 301 
                 tatgcaataa atgcattaaa tgtaacttta ttaaacatag tacactgtac ttgacttatg 
               
               
                   
               
               
                 361 
                 gttaaatatt ttacacacag cttga 
               
               
                   
               
             
          
           
               
                 50: NM_014785   KIAA0258 
                   
               
             
          
           
               
                 1 
                 gccaggtccc tgaggggcgg gcagatgagg cctaggggtg ccgatcccta gtgtcgacta 
                   
               
               
                   
               
               
                 61 
                 tgcgagatct gattccggag ctgccatgat tgaagtggta gcagagctca gccggggtcc 
               
               
                   
               
               
                 121 
                 tgtatttttg gctggggagg cgctggagtg tgtagtgacc gtcaccaacc cccttccgcc 
               
               
                   
               
               
                 181 
                 cacggccact tctgcatcca gtgaggccct ggcctgggcc agtgcccaaa tccactgcca 
               
               
                   
               
               
                 241 
                 gttccatgcc agtgagagtc gagtagcact gcctcctcct gactctagtc agccagatgt 
               
               
                   
               
               
                 301 
                 ccagcccgac agccagactg tctttctgcc acaccgaggt gagaggggcc agtgtatcct 
               
               
                   
               
               
                 361 
                 ttctactcca ccgaaaattc tattctgtga cctgaggctt gatcctggag agtccaaatc 
               
               
                   
               
               
                 421 
                 atactcctac agtgaagtgc tgcccataga gggaccaccc tcctttcggg gtcagtcagt 
               
               
                   
               
               
                 481 
                 caagtacgtc tacaaactga ccattggctg ccagcgtgtc aactccccta tcactttact 
               
               
                   
               
               
                 541 
                 cagagtccct ctgagggttc ttgtgctgac tggccttcag gatgtccggt ttccccagga 
               
               
                   
               
               
                 601 
                 tgaggctgta gccccatcca gtccattctt ggaggaggat gaaggtggga agaaagattc 
               
               
                   
               
               
                 661 
                 atggctagct gagctggctg gggaacgcct aatggctgcc acatcctgcc gcagcctcca 
               
               
                   
               
               
                 721 
                 tctatacaat atcagtgatg gccgagggaa agttgggacg tttggcatct tcaaatctgt 
               
               
                   
               
               
                 781 
                 gtacagactt ggcgaggacg tggtggggac cttaaactta ggggaaggaa ccgtagcttg 
               
               
                   
               
               
                 841 
                 tttgcagttt tcagtcagct tacagaccga ggagcgtgta cagcctgagt accagcggcg 
               
               
                   
               
               
                 901 
                 acgtggggca gggggtgtcc cctctgtgtc acatgtgact cacgcccggc accaggaatc 
               
               
                   
               
               
                 961 
                 ctgcctacat acaactagaa ccagcttctc cctcccaatc cctctcagct ccaccccagg 
               
               
                   
               
               
                 1021 
                 cttctgtaca gccattgtgt ccttgaagtg gagattgcat tttgaatttg taacgtcccg 
               
               
                   
               
               
                 1081 
                 agaaccagga ttggtactcc taccccctgt ggaacagccc gaacctacca cctggacagg 
               
               
                   
               
               
                 1141 
                 acctgagcaa gtacctgtag acaccttcag ctgggacctg cccatcaagg tgctgcctac 
               
               
                   
               
               
                 1201 
                 tagccccacc ctggcctcat atgctgcccc aggccccagc accagcacca taaccatctg 
               
               
                   
               
               
                 1261 
                 aaactggccc accctggtgc tagttccttc cggatactga gaactcagca cctggactct 
               
               
                   
               
               
                 1321 
                 aatgggaccc actttttcca cctggggtcc aatgtcgtgg acagtgagag tcgggctttc 
               
               
                   
               
               
                 1381 
                 agctatagca ttaatttatt tgttcagaat acattggcag ctgctagtgg tttccctgga 
               
               
                   
               
               
                 1441 
                 agtggcagca gcagtgagca gtcagcagat ggatgatcag ttgagtttag ctggagtggg 
               
               
                   
               
               
                 1501 
                 gagcaggagc cccaggaaca ggggtgttgg ctgagcccca ttctgggtca ggccctcccc 
               
               
                   
               
               
                 1561 
                 ctttgcaggg cagccgaggg tcagattttt gcaccaagga gaactggcag gttcctgcct 
               
               
                   
               
               
                 1621 
                 cctgacgtac ctcacaccca gccgggaagt cgatgggatg ctgggacctg gggaaccaag 
               
               
                   
               
               
                 1681 
                 gataggggaa ggagtcagca cagtgaaagg ctgcctttat ccctgcccac atgttccctc 
               
               
                   
               
               
                 1741 
                 tctcacagtt ttccccccac agagcccctt tcagtggccc cttggtcctc ctaactaagc 
               
               
                   
               
               
                 1801 
                 tgtcacctac catatgtggg cctttttgtt ttataacagg agtattttct ctccaggtcc 
               
               
                   
               
               
                 1861 
                 accccaacct cccctgattt atagcctgaa gccttatctt tcacactagt gttggtccct 
               
               
                   
               
               
                 1921 
                 tcaggtttgg cccatcttgt attgctcttc tgttcattct tacatcacag caatctagtc 
               
               
                   
               
               
                 1981 
                 actccctggt catccctcag tcactcatat cagagtcatt ctctctggcc atctttggtc 
               
               
                   
               
               
                 2041 
                 actcacgtgt cacagcagcc cacgccaaca ggatgcagac aggtgcaatg gaaacagtcc 
               
               
                   
               
               
                 2101 
                 ttgcggagcc aagactcacc cagggtaaaa tatttcccct catagtgaca gggggctagg 
               
               
                   
               
               
                 2161 
                 gaagaacggg aaatgttagt aggtgtagga gtgctgatga gaggcagagg ctcttctggt 
               
               
                   
               
               
                 2221 
                 ctggggtgga gacagtaagt acgcactatc cccgtattta gtttgtcttt cctgtttcac 
               
               
                   
               
               
                 2281 
                 agctggagga agcctgggta ttttgacacg ggatcatctg taaggcccca tcctccctgt 
               
               
                   
               
               
                 2341 
                 gccctctctg ctgctcctcc attcctaacg cttcacccca ctttaccttg agcttggaag 
               
               
                   
               
               
                 2401 
                 tagcacttgc tgtagactcc tgggtgctgg aggagtagag acatcaccaa gcagatgatc 
               
               
                   
               
               
                 2461 
                 ccccagcctc ctaggatccc cttggcctgt ccagcccaga gcatccttag ggccattgct 
               
               
                   
               
               
                 2521 
                 gctgcacagc cctctcagac ccttcttggc ctctgctcag ctactctggt cttgactcct 
               
               
                   
               
               
                 2581 
                 tgactttgct ttgcgttgct ccttgagtct tagtttctgt ctttctcccc tgggctcctg 
               
               
                   
               
               
                 2641 
                 tctcacacta tctccctgcc ctctgctctc acaggctggg gatgtttata aagtgaggac 
               
               
                   
               
               
                 2701 
                 cctggccccc tgctgagtag agctggaaaa gttgtaactc tgtttcctga ggtgagggca 
               
               
                   
               
               
                 2761 
                 tgaaaacaag aggtctagct ttaacaagct gtgagagctg attcatgccc cggcacagct 
               
               
                   
               
               
                 2821 
                 agagggaggg aggtggccat ggagggggca ctggactggg cacttcccca gcaaggaggc 
               
               
                   
               
               
                 2881 
                 aggaggggcg agggccccca ggtggtcccc agatctcttc cctgacctgg agagaaggaa 
               
               
                   
               
               
                 2941 
                 gcattccacc ttcccccttt ctcccccact gccaccacca ggggtgtgta tgctgggatc 
               
               
                   
               
               
                 3001 
                 cctgcctgga ccggagggag gcatttcctg gggatggtta atcctgtgcc ccagccaaac 
               
               
                   
               
               
                 3061 
                 ccaggagctg caatagggtg cgacggccag aagctccagg agagtgagca ggcacctgga 
               
               
                   
               
               
                 3121 
                 gtggagactg tgtttccctc agatcctagg gcagggtttc cctaatgtat ccaagaaata 
               
               
                   
               
               
                 3181 
                 gggctgcccc tcagagatgg tggggagggt ctcttttcct caggcattcc agaggtgaac 
               
               
                   
               
               
                 3241 
                 tgtccattgc ttatcacctt caaacataca gcagatgtgg gatcacccca catctgggga 
               
               
                   
               
               
                 3301 
                 tggttctttc ccctttcaaa gaggagcatc tctaagtgcc ctgatgggat gaatcactcc 
               
               
                   
               
               
                 3361 
                 aggttcacag aggtgtcctc tctttcctcc catatataat ggagtgaggt ttttaggaat 
               
               
                   
               
               
                 3421 
                 ttatcatttg gcatcctctg agtttcccac aggttctgga ggagcccagg atggattatt 
               
               
                   
               
               
                 3481 
                 gagagcatgg gctgtagaga cagtcttctt ggattcagat cctgactcca cttagctatg 
               
               
                   
               
               
                 3541 
                 taacctggtc agattacttc acctctctga gcctgtttcc tcatctataa attggggata 
               
               
                   
               
               
                 3601 
                 gtaatgccaa ctcattgggc tgttatgagg attactgaga taatgcgtgc agtgctctta 
               
               
                   
               
               
                 3661 
                 tcaccatctc tggtgcgtaa gcgtcaggaa atagcagttg ctgtgattgg ggctaaagct 
               
               
                   
               
               
                 3721 
                 ctgaggcaaa atgggcgaca ttattttctt tgaatgacat taagcagttt gtgcatagct 
               
               
                   
               
               
                 3781 
                 gagggcttct attggggatg gctgtctcct ggcatagacc tctgcacctt tcacactcat 
               
               
                   
               
               
                 3841 
                 actccttgtc agcagtcccc aacctttttg gtaccaggga ccggttttgt ggaaaacaat 
               
               
                   
               
               
                 3901 
                 ttttccacca gtggatggag ggggatagca gcggggagat gattttggga tgaaactgtt 
               
               
                   
               
               
                 3961 
                 tcatctcaga tcatcaggca ttagattctc ataaggagtg tgcaatctag atcccttgca 
               
               
                   
               
               
                 4021 
                 tgcggagttc acagtggggt ttgcactcct gtgagaatct aatgcctctg ctgatctgcc 
               
               
                   
               
               
                 4081 
                 aggaggagga gctcaggcgg taatgctcac tcgcctgccg cccacctcct gctttgtgct 
               
               
                   
               
               
                 4141 
                 cccgcttcct aacaggccac agactggtac tggcctgtgg cctgggggat ggagacccct 
               
               
                   
               
               
                 4201 
                 aatccatgtc acctttccca cctctttcaa aaacaggtac ctccaggaac attttggttt 
               
               
                   
               
               
                 4261 
                 tggcccttgt attgacttct gaatgtctag tttgagaaac tgttcccaaa taagccttct 
               
               
                   
               
               
                 4321 
                 tcccccagat ctgcaccctc gcctctaccc taggacaaga tgtccttttc tcatcatcct 
               
               
                   
               
               
                 4381 
                 gccaggctaa ctttaagtct cctgcttttt ctcacttgga tttggatcca tttcttccta 
               
               
                   
               
               
                 4441 
                 tttccgctca tgtgaactct ccagttctcc tttctcacca ctctcctgct agccatctct 
               
               
                   
               
               
                 4501 
                 ttggcactaa aggccctggt caaattggat ttctttcatt tttccacact tcaaagaccc 
               
               
                   
               
               
                 4561 
                 atgttctagg tattctccat agggatagtc tctttggcat ttatttggtt tttctacgtt 
               
               
                   
               
               
                 4621 
                 ttcagtccca tttactccaa gactcactcc ctgccaccta gtgcatcaga tacagctact 
               
               
                   
               
               
                 4681 
                 tctggctgac ttttcaaggg ggaccaccct acctgtcatc tcttcactgt tcagaaatga 
               
               
                   
               
               
                 4741 
                 ctgtgtcagt gcacctcaaa ctcccttgct gtccttttcc aaggagacag ctaaggtgga 
               
               
                   
               
               
                 4801 
                 tggagatgca gaatggacct cacgttcgcc ctagtcagga ctgataccct ttccgtttca 
               
               
                   
               
               
                 4861 
                 gaggattgcc aagaaaaaac tcacagttga ggcagggtgc tctgaggtcg gctgcggtgt 
               
               
                   
               
               
                 4921 
                 gggaggcacg gcctgggcct gctctctggg ctggagcagg tggattcgaa ggcctgtcta 
               
               
                   
               
               
                 4981 
                 gcacgagggc ccaaaggtct tgtcagtggc cagtagctct gccgcctttc ccagagaggg 
               
               
                   
               
               
                 5041 
                 ggtccagggg acatcctgga aggctgggcc ctgggccacc ttctgctctt gcaagctaga 
               
               
                   
               
               
                 5101 
                 gccagcccaa tagggggcgg atgtgagtgg ggagctgggg cgcatgaagg tgggggtgat 
               
               
                   
               
               
                 5161 
                 gccgaagggg aagggatcgc cagtggggat tggtgcgtgt gcggaaacgg ggacagaagt 
               
               
                   
               
               
                 5221 
                 gaaggttcat cgcctataac gaagatgagg taggcatata ggggcttctg gaaagctaga 
               
               
                   
               
               
                 5281 
                 ggctgggctg agccaggagt cctctcccag aagttggggg gcggtgcaga ggtgtgggtc 
               
               
                   
               
               
                 5341 
                 gagcccgcat gcgtgcctgc tggggagggg gtgagtggtg aggaccaggc ccgctgggtc 
               
               
                   
               
               
                 5401 
                 ctgggggcgc ggtggctggc gcgcaggtcc cggagggggc ggctggcgcg cactacacgc 
               
               
                   
               
               
                 5461 
                 ttgggaacaa ggaaaacatc cgccggaggc ccggccgggc ggcgctccag cctcggggca 
               
               
                   
               
               
                 5521 
                 ggtgcgcgga gaggaagtga gagcattccg gcccccccac cccaaccccg gccgctggcc 
               
               
                   
               
               
                 5581 
                 ctctggtgag tcacagccga cccccgccgc cggagggaga ggggagctgc gggccagagc 
               
               
                   
               
               
                 5641 
                 cccggagggt ctggaggagc caggagggtt tctgggagca gagggtcact tagtgggctt 
               
               
                   
               
               
                 5701 
                 ctgtcgtggt gtcgctacgg gcgcgaaacg gacactgaac acagtctgac tgtatggagg 
               
               
                   
               
               
                 5761 
                 caggtgggga gggatcccct gggagaactt ggcgggccga gagcagaccc cagggcaagg 
               
               
                   
               
               
                 5821 
                 aggggccccc gagggggaaa ccgggagtcg ggcaggtggc gtaacccaga aagggaagga 
               
               
                   
               
               
                 5881 
                 gagccggatt gattggggtg agagaggaag gaagcacgcc aagttaggcc tgggagaact 
               
               
                   
               
               
                 5941 
                 gagggacctg aggagggagg agggagacca acacagggtg ggaaggcgga aatggccaaa 
               
               
                   
               
               
                 6001 
                 ccccaggcat caggtctgtc cagaggctga cgtagacagt gaagggtgaa gggtaggttt 
               
               
                   
               
               
                 6061 
                 taggagtagg gggagttatg attatttggt tacattttgg gattatttgg tctcacaggt 
               
               
                   
               
               
                 6121 
                 agaagggagc ctgctggtct ctgtgtaacg gatggcttaa aagcaaggtt gtctgcgtct 
               
               
                   
               
               
                 6181 
                 tggattactg tctgccattc agcctttgcc aaaaaatttg gcactgatct gcacattttt 
               
               
                   
               
               
                 6241 
                 atagtcattt aaaattgtat gactctgtca aatgatttaa gtaattttgg tggattttta 
               
               
                   
               
               
                 6301 
                 aaaataaaaa aat 
               
               
                   
               
             
          
           
               
                 51: BF973104   TOM7 
                   
               
             
          
           
               
                 1 
                 ggtaaggggt cctccctgcg ccacacggcc gtcgccatgg tgaagctgag caaagaggcc 
                   
               
               
                   
               
               
                 61 
                 aagcagagac tacagcagct cttcaagggg agccagtttg ccattcgctg gggctttatc 
               
               
                   
               
               
                 121 
                 cctcttgtga tttacctggg atttaagagg ggtgcagatc ccggaatgcc tgaaccaact 
               
               
                   
               
               
                 181 
                 gttttgagcc tactttgggg ataaaggatt atttggtctt ctggatttgg aggcaatcag 
               
               
                   
               
               
                 241 
                 cggacagcat ggaagatgtg tgctctggct cggataagag atgggacatc attcagtcac 
               
               
                   
               
               
                 301 
                 tagttggatg gcacaaggct cttcacagac gcatctgtag cagagtggat cttgtactaa 
               
               
                   
               
               
                 361 
                 cttatgatag aatgtatcag aataaatgtt tttaacagtg taaacaccac aaacaaaaaa 
               
               
                   
               
               
                 421 
                 cacaacacac acatcataca cacaaaaaac acaaaaaaaa caaacaaatc acacaaaagc 
               
               
                   
               
               
                 481 
                 tacggtagac ctactattat gcggtgggcc gaaacaagac gggtattata gacaagggaa 
               
               
                   
               
               
                 541 
                 acgagtcgtc aaatcgtcgt agcctgacac acatcatatt gttagaccca gcgtgtgcaa 
               
               
                   
               
               
                 601 
                 tatctcgccg gggtagctcc ctcatatgag ggacacgtta tatatgtctc agatagggcg 
               
               
                   
               
               
                 661 
                 ccggggtata acctgcagtt ttatagatat gctggcaaca gaaaaaagcg atgtaaaaaa 
               
               
                   
               
               
                 721 
                 aaaaatgaag acaacataaa cacacacaca aagatactat cacatatata ctataaccaa 
               
               
                   
               
               
                 781 
                 aaaatctcaa agcgtaaatc aaaaatacac taaaacaatt cacagccata ttcactacac 
               
               
                   
               
               
                 841 
                 cctatccacc ccacacaaaa aaaataagac acaaaacatc acacatatac acactaccta 
               
               
                   
               
               
                 901 
                 tcattttata ctttaatcta atataattaa gtaacaaatc aacacaaata tacacacgat 
               
               
                   
               
               
                 961 
                 cgatagatac actgataaaa ttcaacaaac aaaataccaa ataaaatata ctaaacacac 
               
               
                   
               
               
                 1021 
                 cactagacga gcatcttata ttgcactttt acgtagacct ctgatcaata acaacagacc 
               
               
                   
               
               
                 1081 
                 tactccacaa atatactact aacacacaaa caatgcaaac agcacagaat aac 
               
               
                   
               
               
                   
               
             
          
           
               
                 Rank 
                 GenBank 
                 Gene 
                   
                   
                   
               
               
                 Order 
                 ID 
                 Symbol 
                 Gene Name 
                 Nucleotides 
               
               
                   
               
             
          
           
               
                 5 
                 NM_004090 
                 DUSP3 
                 dual specificity phos- 
                 GGATCCTTTATTGGTGGTAGAG 
                   
               
               
                   
               
               
                   
                   
                   
                 phatase 3 (vaccinia 
                 CAAAAAAACCCAAACACGATAA 
               
               
                   
               
               
                   
                   
                   
                 virus phosphatase VH1- 
                 ACCTTTCAAAAGACTTTCTAAG 
               
               
                   
               
               
                   
                   
                   
                 related) 
                 GATGATATTGGAATGCACCAGC 
               
               
                   
               
               
                   
                   
                   
                   
                 CCTCACATGTGTATGCACATTT 
               
               
                   
               
               
                   
                   
                   
                   
                 GCCAGAATATAAGAGTTTTGTT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTAAATACAGTCTTGTTAGGAT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTACGTTATTGTTATTATGGA 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGTGATTGTGATGCTATTTAT 
               
               
                   
               
               
                   
                   
                   
                   
                 CTTCAGGGTCACTCTGG 
               
               
                   
               
               
                 6 
                 AI026836 
                 DJ473B4 
                 hypothetical protein 
                 GCAGTCGTTTCAACCAGGTAGT 
               
               
                   
               
               
                   
                   
                   
                 dJ473B4 
                 TTTGGGTTGTTTTTAAAGCCCT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTGAGGTCTTACACATTATTA 
               
               
                   
               
               
                   
                   
                   
                   
                 ACTTTAAAATAATCAGGCAGCT 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGAATAATTACTAGAAAAATC 
               
               
                   
               
               
                   
                   
                   
                   
                 ATCTACCACTTCAAACATGGTC 
               
               
                   
               
               
                   
                   
                   
                   
                 AACTACTTCAAAACTGCACCTA 
               
               
                   
               
               
                   
                   
                   
                   
                 GAGAATCAGGTACCTGAAGTAG 
               
               
                   
               
               
                   
                   
                   
                   
                 AACAAGAAGCCTGGAGGTGGAC 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTGAGAGGAGGGAATACCC 
               
               
                   
               
               
                 7 
                 BU500509 
                 PHLDA2 
                 pleckstrin homology-like 
                 TACGTGTACTTCACCATCGTCA 
               
               
                   
               
               
                   
                   
                   
                 domain, family A, member 
                 CCACCGACCACAAGGAGATCGA 
               
               
                   
               
               
                   
                   
                   
                 2 
                 CTTCCGCTGCGCGGGCGAGAGC 
               
               
                   
               
               
                   
                   
                   
                 TGCTGGAACGCGGCCATCGCGC 
               
               
                   
               
               
                   
                   
                   
                   
                 TGGCGCTCATCGATTTCCAGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 CCGCCGCGCCCTGCAGGACTTT 
               
               
                   
               
               
                   
                   
                   
                   
                 CGCAGCCGCCAGGAACGCACCG 
               
               
                   
               
               
                   
                   
                   
                   
                 CACCCGCCGCACCCGCCGAGGA 
               
               
                   
               
               
                   
                   
                   
                   
                 CGCCGTGGCTGCCGCGGCCGCC 
               
               
                   
               
               
                   
                   
                   
                   
                 GCACCCTCCGAGCCCTCGGAGC 
               
               
                   
               
               
                   
                   
                   
                   
                 CCTCCAGGCCATCCCCGCAGCC 
               
               
                   
               
               
                   
                   
                   
                   
                 CAAACCCCGCACGCCATGAGCC 
               
               
                   
               
               
                   
                   
                   
                   
                 CGCCGCGGGCCATACGCTGGAC 
               
               
                   
               
               
                   
                   
                   
                   
                 GAGTCGGACCGAGGCTAGGACG 
               
               
                   
               
               
                   
                   
                   
                   
                 TGGCCGGCGCTCTCCAGCCCTG 
               
               
                   
               
               
                   
                   
                   
                   
                 CAGCAGAAGAACTTCCCGTGCG 
               
               
                   
               
               
                   
                   
                   
                   
                 CGCGGATCCTCGCTCCGTTGCA 
               
               
                   
               
               
                   
                   
                   
                   
                 CGGGCGCCTTAAGTTATTGGAC 
               
               
                   
               
               
                   
                   
                   
                   
                 TATCTAATATCTATGTATTTAT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTCGCTGGTTCTTTGTAGTCAC 
               
               
                   
               
               
                   
                   
                   
                   
                 ATATTTTATAGTCTTAATATCT 
               
               
                   
               
               
                   
                   
                   
                   
                 TGTTTTTGCATCACTGTGCCCA 
               
               
                   
               
               
                   
                   
                   
                   
                 TTGCAAATAAATCACTTGGCCA 
               
               
                   
               
               
                   
                   
                   
                   
                 GTTTGCTTTTCTACCATCC 
               
               
                   
               
               
                 8 
                 NM_016090 
                 RBM7 
                 RNA binding motif 
                 CTGTGACATGCTCTTGAGCTTT 
               
               
                   
               
               
                   
                   
                   
                 protein 7 
                 ACCCTAGTTGAACATACATGTG 
               
               
                   
               
               
                   
                   
                   
                   
                 TAGATTTACACATACTGTTTCA 
               
               
                   
               
               
                   
                   
                   
                   
                 TTNNNNAATTTAGAAATTGTTC 
               
               
                   
               
               
                   
                   
                   
                   
                 ATTAAATCCCATTTGAGGTATA 
               
               
                   
               
               
                   
                   
                   
                   
                 AGTCACTCAGGAAGTTAAAATA 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTCTACACGTATATTTTTACA 
               
               
                   
               
               
                   
                   
                   
                   
                 TTAAAAATACAGTGTTAGCATA 
               
               
                   
               
               
                   
                   
                   
                   
                 ANNNNCCCTTTNNNNNGAAGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 CAAAAATGTCAGTGCATAGTTA 
               
               
                   
               
               
                   
                   
                   
                   
                 GATAAAATGGTAAAATGTTTTA 
               
               
                   
               
               
                   
                   
                   
                   
                 CTGAAAGCATACTTTTTTGGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 AATAGATTCATGAAGCCTTTAA 
               
               
                   
               
               
                   
                   
                   
                   
                 GTGCTGCTTCTGTCAGTCAAAC 
               
               
                   
               
               
                   
                   
                   
                   
                 GTTAAAAACTTTAACATTTTCA 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGTGCCCAGAGTGTGTACAAA 
               
               
                   
               
               
                   
                   
                   
                   
                 GACACATGTAATGGAGATTGTA 
               
               
                   
               
               
                   
                   
                   
                   
                 CAGGTTGTTTTTTTGTTTGAAC 
               
               
                   
               
               
                   
                   
                   
                   
                 CTTTGAAAGAGTTTAATCTTAA 
               
               
                   
               
               
                   
                   
                   
                   
                 CGTTTTCTAATTTTAAAATTTT 
               
               
                   
               
               
                   
                   
                   
                   
                 AAAATCTTGTTTAACAAAAGCT 
               
               
                   
               
               
                   
                   
                   
                   
                 TGTATTAAGATACTGTTTTCAT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTCATTACAGAATTGTTTATAA 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGTTCATTTGTTGAAAANNNA 
               
               
                   
               
               
                   
                   
                   
                   
                 GGATCCTTTTTAATACCACAGC 
               
               
                   
               
               
                   
                   
                   
                   
                 ATTTGTACTGTTCCT 
               
               
                   
               
               
                 9 
                 BX092512 
                 EST 
                   
                 ATATGTGCACACACACACTCAC 
               
               
                   
               
               
                   
                   
                   
                   
                 ACCCACACCCATAAAGATTTTG 
               
               
                   
               
               
                   
                   
                   
                   
                 CACTCCTTGAAGGTACACTAAC 
               
               
                   
               
               
                   
                   
                   
                   
                 TCACCATTTTTATCATACTTAT 
               
               
                   
               
               
                   
                   
                   
                   
                 CCCAGTGTGCCACAGTTACTGG 
               
               
                   
               
               
                   
                   
                   
                   
                 CTTATATGCCTGTCTCTGCTAT 
               
               
                   
               
               
                   
                   
                   
                   
                 CTTATTTTATCTGTCTCCACAA 
               
               
                   
               
               
                   
                   
                   
                   
                 CACAGCAAACTACCTGGCCTTC 
               
               
                   
               
               
                   
                   
                   
                   
                 AATAAAGGGCTTATGAATTATT 
               
               
                   
               
               
                   
                   
                   
                   
                 CATGAATCCATTTTGCCAGGTG 
               
               
                   
               
               
                   
                   
                   
                   
                 CCTAGCCCTGTGTCTGGCTTGA 
               
               
                   
               
               
                   
                   
                   
                   
                 AGCAGGTGTTCCCAAGGTGTGG 
               
               
                   
               
               
                   
                   
                   
                   
                 CATGGCTGAGTGAATACAAAT 
               
               
                   
               
               
                 10 
                 AI436027 
                 OSMR 
                 oncostatin M receptor 
                 CACCAATGAGCTTACTACCCAA 
               
               
                   
               
               
                   
                   
                   
                   
                 CTTCAAAACTAGGACTCTAACA 
               
               
                   
               
               
                   
                   
                   
                   
                 ATAACTTCTGTCATATCTCATC 
               
               
                   
               
               
                   
                   
                   
                   
                 CTGTAACGCCCCCACCTTCGCT 
               
               
                   
               
               
                   
                   
                   
                   
                 CCTTCCGCCAAGATAATTATCA 
               
               
                   
               
               
                   
                   
                   
                   
                 CTTTAAATTGTGTGCGTGTGTA 
               
               
                   
               
               
                   
                   
                   
                   
                 TTCTCATTTCTTATGTGATGGT 
               
               
                   
               
               
                   
                   
                   
                   
                 AAAAATGCCTTTATTTTGTTTG 
               
               
                   
               
               
                   
                   
                   
                   
                 GTTTTAATGCATAGAAAGGACA 
               
               
                   
               
               
                   
                   
                   
                   
                 TCAAGCTGT 
               
               
                   
               
               
                 11 
                 AI971137 
                 GCLC 
                 glutamate-cysteine li- 
                 CTCTAAAAGCCATTCACTCCAG 
               
               
                   
               
               
                   
                   
                   
                 gase, catalytic subunit 
                 ATTTTACCTGGGGAATATTCTA 
               
               
                   
               
               
                   
                   
                   
                   
                 CATACTGCTTACTTTCTCTATA 
               
               
                   
               
               
                   
                   
                   
                   
                 AAACTCATCAATAAATCATGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 AGGCACTGAGTTTTGTAAATCA 
               
               
                   
               
               
                   
                   
                   
                   
                 GGACCCTAAATGTTTAATTGTA 
               
               
                   
               
               
                   
                   
                   
                   
                 AATAAGTTTCAGATAATTATTA 
               
               
                   
               
               
                   
                   
                   
                   
                 TAGCTTTGCGTTGAAGTTNNNN 
               
               
                   
               
               
                   
                   
                   
                   
                 NNNNNTTTCTCTCAACTAGTTA 
               
               
                   
               
               
                   
                   
                   
                   
                 AGTCAACTGCTTCTGAAATAAC 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTGTATTGTAGATTATGCAGA 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTTTACAGGCATAAATATTTA 
               
               
                   
               
               
                   
                   
                   
                   
                 AACTGTAATATGCTAACTTGAA 
               
               
                   
               
               
                   
                   
                   
                   
                 GAGATTGCAATAAAGCTGCTTC 
               
               
                   
               
               
                   
                   
                   
                   
                 AGCTAAC 
               
               
                   
               
               
                 12 
                 BQ024877 
                 COL4A3BP 
                 collagen, type IV, alpha 
                 CTCACTGAAGTTGAAATGACTG 
               
               
                   
               
               
                   
                   
                   
                 3 (Goodpasture antigen) 
                 CCCACTTCAAAATCTTCATTGT 
               
               
                   
               
               
                   
                   
                   
                 binding protein 
                 GTTTACACACCAGTGTATTTAT 
               
               
                   
               
               
                   
                   
                   
                 ACAAATCAGAGGCATTTTGTAG 
               
               
                   
               
               
                   
                   
                   
                   
                 ATGCTTTGCTGACTTGTTCAGC 
               
               
                   
               
               
                   
                   
                   
                   
                 TCTGTAAAAACACAGAAATCAG 
               
               
                   
               
               
                   
                   
                   
                   
                 ACCCATTTTGTAAAGCGGAAAA 
               
               
                   
               
               
                   
                   
                   
                   
                 TCATGTTACATGGAACATGTCC 
               
               
                   
               
               
                   
                   
                   
                   
                 TGTATATATCACATACATGGTA 
               
               
                   
               
               
                   
                   
                   
                   
                 ATGGAGTCTTAATGATAAGTGC 
               
               
                   
               
               
                   
                   
                   
                   
                 AAGATAATAATTTAATGATGGG 
               
               
                   
               
               
                   
                   
                   
                   
                 ATTAGTCTGATCGCTTAATATG 
               
               
                   
               
               
                   
                   
                   
                   
                 CACAATCCTGGAAGTGAATTAC 
               
               
                   
               
               
                   
                   
                   
                   
                 TTGCATCAGATATAGTGATATT 
               
               
                   
               
               
                   
                   
                   
                   
                 TATTATTCTGTACAGAGAGAAA 
               
               
                   
               
               
                   
                   
                   
                   
                 AATACATATAAAACATATGCTT 
               
               
                   
               
               
                   
                   
                   
                   
                 ACATTACATGCACGCGGATTTC 
               
               
                   
               
               
                   
                   
                   
                   
                 ATGCTCCATAATCTTTTCTATT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTTTAATTTACCTTTCTGTAAA 
               
               
                   
               
               
                   
                   
                   
                   
                 TGATGTGCATGGAATATGCCTT 
               
               
                   
               
               
                   
                   
                   
                   
                 ATAGAAAAATGCTGTTCATAAT 
               
               
                   
               
               
                   
                   
                   
                   
                 TTGACTACGTGGAAAAGTGCCT 
               
               
                   
               
               
                   
                   
                   
                   
                 ATATGGTGGTAATGCTAGTAAG 
               
               
                   
               
               
                   
                   
                   
                   
                 GCA 
               
               
                   
               
             
          
         
       
     
         [0171]    
       
         
               
             
               
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 5A 
               
             
             
               
                   
               
               
                   
               
               
                 Correlation of cDNA microarray data with semi-quantatative RT-           
               
             
          
           
               
                   
                 Spearman 
                   
               
               
                   
                 rank correlation 
               
             
          
           
               
                 Rank Order 
                 Gene Symbol 
                 ρ 
                 p-value 
               
               
                   
               
             
          
           
               
                 1 
                 FLJ22662 
                 0.69 
                 0.02 
               
               
                 2 
                 AREG 
                 0.53 
                 0.08 
               
               
                 3 
                 CORO1C 
                 0.35 
                 0.24 
               
               
                 4 
                 AVEN 
                 0.63 
                 0.04 
               
               
                 5 
                 DUSP3 
                 0.63 
                 0.04 
               
               
                 6 
                 DJ473B4 
                 0.45 
                 0.14 
               
               
                 7 
                 PHLDA2 
                 0.84 
                 0.01 
               
               
                 8 
                 RBM7 
                 0.83 
                 0.01 
               
               
                 9 
                 EST(BX092512) 
                 0.63 
                 0.04 
               
               
                 10 
                 OSMR 
                 0.67 
                 0.03 
               
               
                 11 
                 GCLC 
                 0.46 
                 0.13 
               
               
                 12 
                 COL4A3BP 
                 0.27 
                 0.24 
               
               
                   
               
               
                   Correlations positive for all 12 genes and significantly positive for 7 of 12 ge             
               
             
          
         
       
     
         [0172]    
       
         
               
             
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 5B 
               
             
             
               
                   
               
               
                   
               
               
                 Result of immunohistochemical staining 
               
             
          
           
               
                   
                 PR 
                 PD 
               
               
                   
                   
               
             
          
           
               
                   
                 AREG 
                 1/5 
                 5/6 
               
               
                   
                 TGFA 
                 2/5 
                 6/6 
               
               
                   
                 ADAM9 
                 1/5 
                 4/6 
               
               
                   
                 CD9 
                 2/5 
                 5/6 
               
               
                   
                 OSMR 
                 2/5 
                 6/6 
               
               
                   
                   
               
             
          
         
       
     
         [0173]