PATENT ABSTRACT
Polynucleotide sequences encoding novel cadherin-like polypeptides, designated protocadherins, and variants thereof are provided by the invention as well as methods and materials for the recombinant production of the same. Antibody substances specific for protocadherins are also disclosed as useful for modulating the natural binding and/or regulatory activities of the protocadherins.

PATENT DESCRIPTION
[0001]    This application is a continuation-in-part of International Patent Application No. PCT/US93/12588 filed Dec. 23, 1993 which is in turn a continuation-in-part of U.S. patent application Ser. No. 07/998,003 which was filed on Dec. 29, 1992. 
     
    
     
       FIELD OF THE INVENTION  
         [0002]    The present invention relates, in general, to materials and methods relevant to cell-cell adhesion. More particularly, the invention relates to novel adhesion proteins, designated protocadherins, and to polynucleotide sequences encoding the protocadherins. The invention also relates to methods for inhibiting binding of the protocadherins to their natural ligands/antiligands.  
         BACKGROUND  
         [0003]    In vivo, intercellular adhesion plays an important role in a wide range of events including morphogenesis and organ formation, leukocyte extravasion, tumor metastasis and invasion, and the formation of cell junctions. Additionally, cell-cell adhesion is crucial for the maintenance of tissue integrity.  
           [0004]    Intercellular adhesion is mediated by specific cell surface adhesion molecules. Cell adhesion molecules have been classified into at least four families including the immunoglobulin superfamily, the integrin superfamily, the selectin family and the cadherin superfamily. All cell types that form solid tissues express some members of the cadherin superfamily suggesting that cadherins are involved in selective adhesion of most cell types.  
           [0005]    Cadherins have been generally described as glycosylated integral membrane proteins that have an N-terminal extracellular domain (the N-terminal 113 amino acids of the domain appear to be directly involved in binding) consisting of five subdomains characterized by sequences unique to cadherins, a hydrophobic membrane-spanning domain and a C-terminal cytoplasmic domain that interacts with the cytoskeleton through catenins and other cytoskeleton-associated proteins. Some cadherins lack a cytoplasmic domain, however, and appear to function in cell-cell adhesion by a different mechanism than cadherins having a cytoplasmic domain. The cytoplasmic domain is required for the adhesive function of the extracellular domain in cadherins that do have an cytoplasmic domain. Binding between members of the cadherin family expressed on different cells is homophilic (i.e., a member of the cadherin family binds to cadherins of its own or a closely related subclass) and Ca 2+ -dependent. For recent reviews on cadherins, see Takeichi,  Annu. Rev. Biochem.,  59:237-252 (1990) and Takeichi,  Science,  251:1451-1455 (1991).  
           [0006]    The first cadherins to be described (E-cadherin in mouse epithelial cells, L-CAM in avian liver, uvomorulin in the mouse blastocyst, and CAM 120/80 in human epithelial cells) were identified by their involvement in Ca 2+ -dependent cell adhesion and their unique immunological characteristics and tissue localization. With the later immunological identification of N-cadherin, which was found to have a different tissue distribution than E-cadherin, it became apparent that a new family of Ca 2+ -dependent cell-cell adhesion molecules had been discovered.  
           [0007]    The molecular cloning of the genes encoding E-cadherin [see Nagafuchi et al.,  Nature,  329:341-343 (1987)], N-cadherin [Hatta et al.,  J. Cell. Biol.,  106:873-881 (1988)], and P-cadherin [Nose et al.,  EMBO J.,  6:3655-3661 (1987)] provided structural evidence that the cadherins comprised a family of cell adhesion molecules. Cloning of L-CAM [Gallin et al.,  Proc. Natl. Acad., Sci. USA,  84:2808-2812 (1987)] and uvomorulin [Ringwald et al.,  EMBO J.,  6:3647-3653 (1986)] revealed that they were identical to E-cadherin. Comparisons of the amino acid sequences of E-, N-, and P-cadherins showed a level of amino acid similarity of about 45%-58% among the three subclasses. Liaw et al.,  EMBO J.,  9. 2701-2708 (1990) describes the use of PCR with degenerate oligonucleotides based on conserved regions of the E-, N- and P-cadherins to amplify N- and P-cadherin from a bovine microvascular endothelial cell cDNA.  
           [0008]    The isolation by PCR of eight additional cadherins was reported in Suzuki et al.,  Cell Regulation,  2:261-270 (1991). Subsequently, several other cadherins were described including R-cadherin [Inuzuka et al.,  Neuron,  7:69-79 (1991)], M-cadherin [Donalies,  Proc. Natl. Acad. Sci. USA  88:8024-8028 (1991)], B-cadherin [Napolitano,  J. Cell. Biol.,  113:893-905 (1991)] and T-cadherin [Ranscht,  Neuron,  7:391-402 (1991)].  
           [0009]    Additionally, proteins distantly related to cadherins such as desmoglein [Goodwin et al.,  Biochem. Biophys. Res. Commun.,  173:1224-1230 (1990) and Koch et al.,  Eur. J. Cell Biol.,  53:1-12 (1990)] and the desmocollins [Holton et al.,  J. Cell Science,  97:239-246 (1990)] have been described. The extracellular domains of these molecules are structurally related to the extracellular domains of typical cadherins, but each has a unique cytoplasmic domain. Mahoney et al.,  Cell,  67:853-868 (1991) describes a tumor suppressor gene of Drosophila, called fat, that also encodes a cadherin-related protein. The fat tumor suppressor comprises 34 cadherin-like subdomains followed by four EGF-like repeats, a transmembrane domain, and a novel cytoplasmic domain. The identification of these cadherin-related proteins is evidence that a large superfamily characterized by a cadherin extracellular domain motif exists.  
           [0010]    Studies of the tissue expression of the various cadherin-related proteins reveal that each subclass of molecule has a unique tissue distribution pattern. For example, E-cadherin is found in epithelial cells while N-cadherin is found in neural and muscle cells. Expression of cadherin-related proteins also appears to be spatially and temporally regulated during development because individual proteins appear to be expressed by specific cells and tissues at specific developmental stages [for review see Takeichi (1991), supra]. Both the ectopic expression of cadherin-related proteins and the inhibition of native expression of cadherin-related proteins hinders the formation of normal tissue structure [Detrick et al.,  Neuron,  4:493-506 (1990); Fujimori et al.,  Development,  110. 97-104 (1990); Kintner,  Cell,  69:225-236 (1992)].  
           [0011]    The unique temporal and tissue expression pattern of the different cadherins and cadherin-related proteins is particularly significant when the role each subclass of proteins may play in vivo in normal events (e.g., the maintenance of the intestinal epithelial barrier) and in abnormal events (e.g., tumor metastasis or inflammation) is considered. Different subclasses or combinations of subclasses of cadherin-related proteins are likely to be responsible for different cell-cell adhesion events in which therapeutic detection and/or intervention may be desirable. For example, auto-antibodies from patients with pemphigus vulgaris, an autoimmune skin disease characterized by blister formation caused by loss of cell adhesion, react with a cadherin-related protein offering direct support for adhesion function of cadherins in vivo [Amagai et al.,  Cell,  67:869-877 (1991)]. Studies have also suggested that cadherins and cadherin-related proteins may have regulatory functions in addition to adhesive activity. Matsunaga et al.,  Nature,  334:62-64 (1988) reports that N-cadherin has neurite outgrowth promoting activity. The Drosophila fat tumor supressor gene appears to regulate cell growth and supress tumor invasion as does mammalian E-cadherin [see Mahoney et al., supra; Frixen et al.,  J. Cell Biol.,  113:173-185 (1991); Chen et al.,  J. Cell, Biol.,  114:319-327 (1991); and Vleminckx et al.,  Cell,  66:107-119 (1991)]. Thus, therapeutic intervention in the regulatory activities of cadherin-related proteins expressed in specific tissues may be desirable.  
           [0012]    There thus continues to exist a need in the art for the identification and characterization of additional cadherin-related proteins which participate in cell-cell adhesion and/or regulatory events. Moreover, to the extent that cadherin-related proteins might form the basis for the development of therapeutic and diagnostic agents, it is essential that the genes encoding the proteins be cloned. Information about the DNA sequences and amino acid sequences encoding the cadherin-related proteins would provide for the large scale production of the proteins by recombinant techniques and for the identification of the tissues/cells naturally producing the proteins. Such sequence information would also permit the preparation of antibody substances or other novel binding molecules specifically reactive with the cadherin-related proteins that may be useful in modulating the natural ligand/antiligand binding reactions in which the proteins are involved.  
         SUMMARY OF THE INVENTION  
         [0013]    The present invention provides cadherin-related materials and methods that are relevant to cell-cell adhesion. In one of its aspects, the present invention provides purified and isolated polynucleotides (e.g., DNA and RNA, both sense and antisense strands) encoding the novel cell adhesion molecules designated herein as protocadherins, including protocadherin-42, protocadherin-43, protocadherin pc3, protocadherin pc4 and protocadherin pcs. Preferred polynucleotide sequences of the invention include genomic and cDNA sequences as well as wholly or partially synthesized DNA sequences, and biological replicas thereof (i.e., copies of the sequences made in vitro). Biologically active vectors comprising the polynucleotide sequences are also contemplated.  
           [0014]    Specifically illustrating protocadherin polynucleotide sequences of the present invention are the inserts in the plasmids pRC/RSV-pc42 and pRC/RSV-pc43 which were deposited with the American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville, Md. 20852 on Dec. 16, 1992 and were assigned ATCC Accession Nos. 69162 and 69163, respectively.  
           [0015]    The scientific value of the information contributed through the disclosures of the DNA and amino acid sequences of the present invention is manifest. For example, knowledge of the sequence of a partial or complete DNA encoding a protocadherin makes possible the isolation by standard DNA/DNA hybridization or PCR techniques of full length cDNA or genomic DNA sequences that encode the protein (or variants thereof) and, in the case of genomic DNA sequences, that specify protocadherin-specific regulatory sequences such as promoters, enhancers and the like. Alternatively, DNA sequences of the present invention may be chemically synthesized by conventional techniques. Hybridization and PCR techniques also allow the isolation of DNAs encoding heterologous species proteins homologous to the protocadherins specifically illustrated herein.  
           [0016]    According to another aspect of the invention, host cells, especially eucaryotic and procaryotic cells, are stably transformed or transfected with the polynucleotide sequences of the invention in a manner allowing the expression of protocadherin polypeptides in the cells. Host cells expressing protocadherin polypeptide products, when grown in a suitable culture medium, are particularly useful for the large scale production of protocadherin polypeptides, fragments and variants thereby enabling the isolation of the desired polypeptide products from the cells or from the medium in which the cells are grown.  
           [0017]    The novel protocadherin protein products of the invention may be obtained as isolates from natural tissue sources, but are preferably produced by recombinant procedures involving the host cells of the invention. The products may be obtained in fully or partially glycosylated, partially or wholly de-glycosylated, or non-glycosylated forms depending on the host cell selected or recombinant production and/or post-isolation processing.  
           [0018]    Protocadherin variants according to the invention may comprise polypeptide analogs wherein one or more of the specified amino acids is deleted or replaced or wherein one or more non-naturally encoded amino acids are added: (1) without loss, and preferably with enhancement, of one or more of the biological activities or immunological characteristics specific for a protocadherin; or (2) with specific disablement of a particular ligand/antiligand binding function. Also contemplated by the present invention are antibody substances (e.g., monoclonal and polyclonal antibodies, chimeric and humanized antibodies, antibody domains including Fab, Fab′, F(ab′) 2 , Fv or single variable domains, and single chain antibodies) which are specific for the protocadherins of the invention. Antibody substances can be developed using isolated natural, recombinant or synthetic protocadherin polypeptide products or host cells expressing such products on their surfaces. The antibody substances may be utilized for purifying protocadherin polypeptides of the invention, for determining tissue expression of polypeptides and as antagonists of the ligand/antiligand binding activities of the protocadherins. Specifically illustrating monoclonal antibodies of the present invention are the protocadherin-43 specific monoclonal antibodies produced by the hybridoma cell line designated 38I2C which was deposited with the ATCC on Dec. 2, 1992 and was assigned ATCC Accession No. HB 11207.  
           [0019]    Numerous other aspects and advantages of the present invention will be apparent upon consideration of the following detailed description, reference being made to the drawing wherein FIG. 1A-C is an alignment of protocadherin amino acid sequences of the invention with the amino acid sequences of N-cadherin and of the Drosophila fat tumor suppressor.  
         DETAILED DESCRIPTION  
         [0020]    The present invention is illustrated by the following examples wherein Examples 1, 2 and 3 describe the isolation by PCR of protocadherin polynucleotide sequences. Example 3 also describes the chromosome localization of several protocadherin genes of the invention. Example 4 describes the isolation by DNA/DNA hybridization of additional protocadherin polynucleotide sequences of the present invention. Example 5 presents the construction of expression plasmids including polynucleotides encoding protocadherin-42 or protocadherin-43 and the transfection of L cells with the plasmids. The generation of antibodies to protocadherin-42 and protocadherin43 is described in Example 6. Example 7 presents the results of immunoassays of transfected L cells for the expression of protocadherin-42 or protocadherin43. Example 8 describes the cell aggregation properties of L cells transfected with protocadherin-42, protocadherin-43 or a chimeric protocadherin-43/E-cadherin molecule. The calcium-binding properties of pc43 are described in Example 9. The results of assays of various tissues and cell lines for the expression of protocadherin-42 and protocadherin-43 by Northern blot, Western blot and in situ hybridization are respectively presented in Examples 10, 11 and 12. Example 13 describes immunoprecipitation experiments identifying a 120 kDa protein that coprecipitates with protocadherin-43. 
       
    
    
     EXAMPLE 1  
       [0021]    The polymerase chain reaction (PCR) was used to isolate novel rat cDNA fragments encoding cadherin-related polypeptides.  
       Design of PCR Primers  
       [0022]    Two regions of conserved amino acid sequence, one from the middle of the third cadherin extracellular subdomain (EC-3) and the other from the C-terminus of the fourth extracellular subdomain (EC-4), were identified by comparison of the published amino acid sequences for L-CAM (Gallin et al., supra), E-cadherin (Nagafuchi et al., supra), mouse P-cadherin (Nose et al., supra), uvomorulin (Ringwald et al., supra), chicken N-cadherin (Hatta et al., supra), mouse N-adherin [Miyatani et al.,  Science,  245:631-635 (1989)] and human P-cadherin [Shimoyama et al.,  J. Cell. Biol.,  109:1787-1794 (1989)], and the corresponding degenerate oligonucleotides respectively set out below in IUPAC-IUB Biochemical nomenclature were designed for use as PCR primers.  
                                   Primer 1 (SEQ ID NO:1)                       5′ AARSSNNTNGAYTRYGA 3′                       Primer 2 (SEQ ID NO:2)                       3′ TTRCTRTTRCGNGGNNN 5′          
 
         [0023]    The degenerate oligonucleotides were synthesized using an Applied Biosystems model 380B DNA synthesizer (Foster City, Calif.  
       Cloning of cDNA Sequences by PCR  
       [0024]    PCR was carried out in a manner similar to that described in Suzuki et al.,  Cell Regulation,  2:261-270 (1991) on a rat brain cDNA preparation. Total RNA was prepared from rat brain by the guanidium isothiocyanate/cesium chloride method described in Maniatis et al., pp. 196 in  Molecular Cloning: A Laboratory Manual,  Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory (1982). Brain poly(A)+ RNAs were then isolated using a FastTrack® kit (Invitrogen, San Diego, Calif.) and cDNA was prepared using a cDNA synthesis kit (Boehringer Mannheim Biochemicals, Indianapolis, Ind.). The PCR reaction was initiated by adding 2.5 units of Taq DNA polymerase (Boehringer Mannheim Biochemicals) to 100 ng template cDNA and 10 μg of each primer, after which 35 reaction cycles of denaturation at 94° C. for 1.5 minutes, annealing at 45° C. for 2 minutes, and polymerization at 72° C. for 3 minutes were carried out. Two major bands of about 450 base pairs (bp) and 130 bp in size were found when the products of the PCR reaction were subjected to agarose gel electrophoresis. The 450 bp band corresponded to the expected length between the two primer sites corresponding to the middle of the third cadherin extracellular subdomain (EC-3) and the carboxyl terminus of the fourth cadherin extracellular subdomain (EC4), but the 130 bp band could not be predicted from any of the previously identified cadherin sequences. The 450 bp and 130 bp bands were extracted by a freezing and thawing method. The resulting fragments were phosphorylated at the 5′ end with T4 polynucleotide kinase and subcloned by a blunt-end ligation into the Sma I site of M13mp18 (Boehringer Mannheim Biochemicals) in a blunt end ligation for sequence analysis. Sequencing of the fragments was carried out by the dideoxynucleotide chain termination method using a Sequenase kit (United States Biochemicals, Cleveland, Ohio). DNA and amino acid sequence were analyzed using the Beckman Microgenie program (Fullerton, Calif.).  
       Analysis of cDNA Sequences  
       [0025]    Nineteen novel partial cDNA clones were isolated. The DNA and deduced amino acid sequences of the clones (including sequences corresponding to the PCR primers) are set out as follows: RAT-123 (SEQ ID NOs: 3 and 4, respectively), RAT-212 (SEQ ID NOs: 5 and 6), RAT-214 (SEQ ID NOs: 7 and 8), RAT-216 (SEQ ID NOs: 9 and 10), RAT-218 (SEQ ID NOs: 11 and 12), RAT-224 (SEQ ID NOs: 13 and 14), RAT-312 (SEQ ID NOs: 15 and 16), RAT-313 (SEQ ID NOs: 17 and 18), RAT-314 (SEQ ID NOs: 19 and 20), RAT-315 (SEQ ID NOs: 21 and 22), RAT-316 (SEQ ID NOs: 23 and 24), RAT-317 (SEQ ID NOs: 25 and 26), RAT-321 (SEQ ID NOs: 27 and 28), RAT-323 (SEQ ID NOs: 29 and 30), RAT-336 (SEQ ID NOs: 31 and 32), RAT-352 (SEQ ID NOs: 33 and 34), RAT411 (SEQ ID NOs: 35 and 36), RAT-413 (SEQ ID NOs: 37 and 38), and RAT-551 (SEQ ID NOs: 39 and 40).  
         [0026]    The deduced amino acid sequences of the cDNA clones are homologous to, but distinct from the known cadherins. The cadherins described thus far have highly conserved, short amino acid sequences in the third extracellular subdomain (EC-3) including the consensus sequence D-Y-E or D-F-E located at the middle region of the subdomain and the consensus sequence D-X-N-E-X-P-X-F (SEQ ID NO:41) or D-X-D-E-X-P-X-F (SEQ ID NO:42) at its end (Hatta et al., supra), while the corresponding sequences of other subdomains, except for the fifth extracellular subdomain (EC-5), are D-R-E and D-X-N-D-N-X-P-X-F (SEQ ID NO:43), respectively. In contrast, the deduced amino acid sequences of the new clones that correspond to cadherin extracellular subdomains include the sequence D-Y-E or D-F-E at one end, but have the sequence D-X-N-D-N-X-P-X-F instead of D-X-N-E-X-P-X-F or D-X-D-E-X-P-X-F, at the other end. The polypeptides encoded by the partial clones are homologous to previously identified cadherins but did not show significant homology to any other sequences in Genbank. Therefore, the partial cDNAs appear to comprise a new subclass of cadherin-related molecules.  
       EXAMPLE2  
       [0027]    Various cDNA fragments structurally similar to the rat cDNAs described in Example 1 were isolated from human, mouse, and Xenopus brain cDNA preparations and from Drosophila and  C. elegans  whole body cDNA preparations by PCR using Primers 1 and 2 as described in Example 1. The DNA and deduced amino acid sequences of the resulting PCR fragments (including sequences corresponding to the PCR primers) are set out as follows: MOUSE-321 (SEQ ID NOs: 44 and 45), MOUSE-322 (SEQ ID NOs: 46 and 47), MOUSE-324 (SEQ ID NOs: 48 and 49), MOUSE-326 (SEQ ID NOs: 50 and 51), HUMAN-11 (SEQ ID NOs: 52 and 53), HUMAN-13 (SEQ ID NOs: 54 and 55), HUMAN-21 (SEQ ID NOs: 56 and 57), HUMAN-24 (SEQ ID NOs: 58 and 59), HUMAN-32 (SEQ ID NOs: 60 and 61), HUMAN-42 (SEQ ID NOs: 62 and 63), HUMAN-43 (SEQ ID NOs: 64 and 65), HUMAN-212 (SEQ ID NOs: 66 and 67), HUMAN-213 (SEQ ID NOs: 68 and 69), HUMAN-215 (SEQ ID NOs: 70 and 71), HUMAN-223 (SEQ ID NOs: 72 and 73), HUMAN-410 (SEQ ID NOs: 74 and 75), HUMAN-443 (SEQ ID NOs: 76 and 77), XENOPUS-21 (SEQ ID NOs: 78 and 79), XENOPUS-23 (SEQ ID NOs: 80 and 81), XENOPUS-25 (SEQ ID NOs: 82 and 83), XENOPUS-31 (SEQ ID NOs: 84 and 85), DROSOPHILA-12 (SEQ ID NOs: 86 and 87), DROSOPHILA-13 (SEQ ID NOs: 88 and 89), DROSOPHILA-14 (SEQ ID NOs: 90 and 91) and C.ELEGANS41 (SEQ ID NOs: 92 and 93). Comparison of the deduced amino acid sequences indicates significant similarity between sets of these clones. In particular, there are three sets of clones that appear to be cross-species homologues: RAT-218, MOUSE-322 and HUMAN-43; RAT-314, MOUSE-321 and HUMAN-11; and MOUSE-326 and HUMAN-42.  
       EXAMPLE 3  
       [0028]    To ascertain the complete structure of the new proteins defined by the PCR products, two full length human cDNAs corresponding to the partial cDNAs HUMAN-42 and HUMAN-43 were isolated.  
       Isolation of Full-length Human cDNAs  
       [0029]    A human fetal brain cDNA library (Stratagene, La Jolla, Calif.) in the λZapII vector was screened by the plaque hybridization method [described in Ausubel et al., Eds.,  Current Protocols in Molecular Biology,  Sections 6.1.1 to 6.1.4 and 6.2.1 to 6.2.3, John Wiley &amp; Sons, New York (1987)] with  32 P-labelled HUMAN-42 and HUMAN-43 DNA fragments. The positive clones were plaque-purified and, using a helper virus, the inserts were cut out by an in vivo excision method in the form of a Bluescript SK(+) plasmid. The insert sequences were then subcloned into the M13 vector (Boehringer Mannheim, Biochemicals) for sequencing. Several overlapping cDNA clones were isolated with each probe including two cDNAs which contained the putative entire coding sequences of two novel proteins designated protocadherin-42 (pc42) and protocadherin-43 (pc43). The DNA and deduced amino acid sequences of pc42 are set out in SEQ ID NOs: 94 and 95, respectively, while the DNA and deduced amino acid sequences of pc43 are set out in SEQ ID NOs: 96 and 97, respectively.  
         [0030]    A description of the cloning of protocadherin sequences of the invention was published in Sano et al.,  The EMBO Journal,  12(6): 2249-2256 (1993) after filing of the priority application hereto. The deduced amino acid sequence of pc43 was previously presented at the Dec. 9, 1991 meeting of the American Society for Cell Biology. An abstract of the presentation is published as Suzuki et al.,  J. Cell. Biol.,  115: 72a (Abstract 416) (Dec. 9, 1991).  
       Analysis of Full-length Human Clones  
       [0031]    Comparison of the full length cDNA sequences of pc42 and pc43 to the sequences of the various DNA fragments originally obtained by PCR reveals that MOUSE-326 and HUMAN-42 correspond to a portion of the fourth extracellular subdomain (EC-4) of pc42, and RAT-314, MOUSE-321, and HUMAN-11 correspond to a portion of the third extracellular subdomain (EC-3) of pc43 and RAT-218, MOUSE-322 and HUMAN-43 correspond to a portion of the fifth extracellular domain (EC-5) of pc43.  
         [0032]    The overall structures of pc42 and pc43 are similar to that of typical cadherins but the new molecules also have distinct features. Both protocadherin cDNA sequences contain putative translation initiation sites and translated amino acid sequences start with typical signal sequences, but the clones lack the prosequences that are present in all known cadherin precursors. The cDNAs encode proteins having a large N-terminal extracellular domain and a relatively short C-terminal cytoplasmic domain connected by a transmembrane sequence. The extracellular domains of pc42 and pc43 are different in length and pc42 contains seven subdomains that closely resemble the typical cadherin extracellular subdomain while pc43 has six such subdomains. The sizes of the protocadherin cytoplasmic domains are similar to those of typical cadherins, but the sequences do not show any significant homology with those of known cadherins or cadherin-related proteins.  
         [0033]    Amino acid identity determinations between extracellular subdomains of human pc42 and pc43, and of mouse N-cadherin (SEQ ID NO: 98) (presented as an example of a “typical” cadherin) and the eighteenth extracellular subdomain of Drosophila fat tumor suppressor (EC-18, SEQ ID NO: 99) (the eighteenth extracellular subdomain of fat is a prototypical fat subdomain) are presented in Table 1 below, wherein, for example, “N-EC-1×pc42” indicates that the first extracellular subdomain of N-cadherin was compared to the extracellular subdomain of pc42 indicated on the horizonal axis.  
                                                                                 TABLE 1                                   EC-1   EC-2   EC-3   EC-4   EC-5   EC-6   EC-7                                    N-EC-1 × pc42   20   27   26   26   31   29   17       N-EC-1 × pc43   31   23   23   26   31   24           N-EC-2 × pc42   28   30   32   30   37   31   19       N-EC-2 × pc43   30   28   30   36   29   30           N-EC-3 × pc42   21   26   30   29   31   30   22       N-EC-3 × pc43   25   18   26   28   28   25           N-EC-4 × pc42   28   28   26   25   29   27   17       N-EC-4 × pc43   21   25   28   28   29   24           N-EC-5 × pc42   24   21   25   24   24   19   12       N-EC-5 × pc43   15   21   20   20   25   16           fat EC-18 × pc42   22   35   32   34   42   35   19       fat EC-18 × pc43   32   30   36   36   33   29                  
 
         [0034]    The amino acid identity values between the extracellular subdomains of pc42 and pc43, and N-cadherin EC-1 through EC-5 and Drosophila fat EC-18 are mostly less than 40%. These identity values are comparable to the values between the subdomains of other cadherin subclasses. However, higher identity values indicate that pc42 and pc43 are more closely related to fat than to N-cadherin.  
         [0035]    Amino acid identity determinations between extracellular subdomains of human pc42 and pc43 are presented in Table 2 below.  
                                                         TABLE 2                                       pc42            pc43   EC-1   EC-2   EC-3   EC-4   EC-5   EC-6   EC-7               EC-1   33   27   29   26   25   26   25       EC-2   26   38   29   33   34   28   21       EC-3   26   32   41   30   32   31   22       EC-4   25   34   30   41   39   31   18       EC-5   23   32   29   27   36   34   16       EC-6   25   25   26   25   28   23   26                  
 
         [0036]    The identity values between respective EC-1, EC-2, EC-3, EC-4, EC-5 subdomains and the last subdomains of pc42 and pc43 are generally higher values than values obtained for comparisons of the protocadherins to N-cadherin. These results suggest that pc42 and pc43 are more closely related to one another than they are to classic cadherins.  
         [0037]    [0037]FIG. 1A-C presents an alignment of the deduced amino acid sequences of the extracellular subdomains of pc42 (EC-1 through EC-7), pc43 (EC-1 through EC-6), mouse N-cadherin (EC-1 through EC-5) and Drosophila fat EC-18. A sequence on a line in FIG. 1A continues on the same line in FIG. 1B and 1C. Gaps were introduced to maximize homology. The amino acid residues described by capital letters in the “motif” line are present in more than half of the subdomains of N-cadherin, pc42, pc43 and Drosophila fat. The amino acid residues described by small letters in the motif line are less well conserved in human pc42, pc43, and Drosophila fat. FIG. 1A-C shows that many amino acids characteristic of other cadherin extracellular domain repeats are conserved in the pc42 and pc43 sequences, including the cadherin sequence motifs DXD, DRE and DXNDNXPXF (SEQ ID NO: 43), two glycine residues, and one glutamic acid residue. Additionally, pc42 and pc43 share unique features in comparison to N-cadherin. More amino acids at specific sites are conserved between pc42 and pc43, such as the DXDXGXN (SEQ ID NO: 100) protocadherin sequence motif near the amino terminus of the pc42 and pc43 subdomains and the AXDXGXP (SEQ ID NO: 101) sequence motif near the carboxyl terminus of the subdomains. Additionally, both protocadherins share regions that do not show significant homology with the typical cadherin motif (of N-cadherin) near the carboxyl terminus of EC-1, in the middle of EC-2 and EC-4, and at the carboxyl terminus of the last repeat. A cysteine residue is located at a similar position in the middle of EC4 of pc42 and pc43. In general, the extracellular subdomains of pc42 and pc43 are more similar to EC-18 of fat than the extracellular subdomains of N-cadherin.  
       Possible Alternative Splicing  
       [0038]    Sequence analysis of various overlapping protocadherin cDNA clones revealed that some clones contained unique sequences at the 3′ end, although the 5′ end sequences were identical to other clones. The sequences forming the boundaries of the 3′ end regions are consistent with the consensus sequence of mRNA splicing, suggesting that these clones may correspond to alternatively spliced mRNAs. The DNA and deduced amino acid sequences of one possible product of alternative splicing of pc42 mRNA are set out in SEQ ID NOs: 102 and 103. The DNA and deduced amino acid sequences of two possible products of alternative splicing of pc43 mRNA are respectively presented in SEQ ID NO: 104 and 105, and SEQ ID NOs: 106 and 107.  
       Chromosome Localization  
       [0039]    The chromosomal location of the protocadherin 413 gene (SEQ ID NO: 37) and of the pc42 and pc43 genes was determined by conventional methods.  
         [0040]    Briefly, C3H/HeJ-gld and  Mus spretus  (Spain) mice and [(C3H/HeJ-gld×Mus spretus) F 1 ×C3H/HeJ-gld] interspecies backcross mice were bred and maintained as previously described in Seldin, et al.,  J. Exp. Med.,  167:688-693 (1988).  Mus spretus  was chosen as the second parent in the cross because of the relative ease of detection of informative restriction fragment length variants (RFLVs) in comparison with crosses using conventional inbred laboratory strains. Gene linkage was determined by segregation analysis.  
         [0041]    Genomic DNA isolated from mouse organs by standard techniques was digested with restriction endonucleases and 10 μg samples were electrophoresed in 0.9% agarose gels. DNA was transferred to Nytran membranes (Schleicher &amp; Schull, Inc., Keene, N.H.), hybridized with the appropriate probe at 65° C and washed under stringent conditions, all as previously described in Maniatis et at., supra). To localize the pc42 gene, a mouse sequence probe corresponding to nucleotides 1419 to 1906 of SEQ ID NO: 94 was used and for pc43 a rat sequence probe corresponding to nucleotides 1060 to 1811 of SEQ ID NO: 96 was used. To localize the procadherin 413 gene, a probe including the sequence set out in SEQ ID NO: 37 was used. Other clones used as probes in the current study and RFLVs used to detect anonymous DNA loci were all previously described (Chromosome 7, DNA segment, Washington 12 (D7Was12); the parathyroid hormone (Pth); calcitonin (Calc); hemoglobin, β chain (Hbb); metallothionein-I (Mt-1); adenine phosphoribosyltransferase (Aprt); growth hormone receptor (Ghr); prostaglandin E receptor EP2 subtype (Ptgerep2); dihydrofolate reductase-2 (Dhfr2); fibroblast growth factor a (Fgfa); and glucocorticoid receptor-1 (Grl-1)].  
         [0042]    Comparison of the haplotype distribution of protocadherin genes with those determined for loci throughout the mouse genome allowed each to be mapped to specific regions of mouse chromosomes. The probability for linkage was &gt;99% and indicated assignment of both the pc42 gene and the pc43 gene was chromosome 18. The assignment of the protocadherin 413 gene was chromosome 7. The region of chromosome 18 to which the pc42 and pc43 genes were mapped corresponds to the ataxia (ax) loci [Burt,  Anat. Rec.,  196: 61-69 (1980) and Lyon, J. Hered., 46:77-80 (1955)] and twirler (TW) loci [Lyon, J.  Embryol. Erp. Morphol.,  6:105-116 (1958)], while the region of chromosome 7 to which the protocadherin 413 gene was mapped corresponds to the shaker (sh-1) locus [Kikuchi et al.,  Acta Oto - Laryngol.,  60:287-303 (1965) and Lord et al.,  Am. Nat.,  63:453-442 (1929)]. These loci have been implicated as involved in hereditary neural disease in the mouse. This result is consistent with in situ hybridization results (see Example 12) showing that pc42 and pc43 are strongly expressed in the brain and particularly in the cerebellum.  
       EXAMPLE 4  
       [0043]    Two additional novel human protocadherin cDNAs and one additional novel rat protocadherin cDNA were isolated using rat protocadherin fragments described in Example 1 as probes.  
         [0044]    Initially, the rat clone RAT-214 (SEQ ID NO: 7) was used as a probe to screen a rat brain cDNA library (Stratagene, La Jolla, Calif.). The final washing step was performed twice at 50° C. in 0.1×SSC with 0.1% SDS for 15 minutes. Various clones were identified which contained partial cDNA inserts encoding related protocadherin amino acid sequences. The nucleotide sequence of one novel rat clone designated #6-2 is set out in SEQ ID NO: 108. The first fifteen nucleotides of SEQ ID NO: 108 are the sequence of a linker and are not part of the rat #6-2 clone.  
         [0045]    A human fetal brain cDNA library obtained from Stratagene was screened with the 0.7 kbp PstI fragment of clone #6-2. The fragment appears to encode the EC-2 and EC-3 of the rat protocadherin. After screening about 2×10 6  phages, eleven positive clones were isolated. Sequencing of the clones identified a novel full length human protocadherin cDNA designated human pc3. The nucleotide and deduced amino acid sequence of human pc3 are set out in SEQ ID NOs: 109 and 110.  
         [0046]    The 0.7 kbp PstI fragment of rat clone #6-2 was also used to rescreen the Stratagene rat brain cDNA library for full length rat cDNA clones. A clone containing an insert encoding a full length novel protocadherin cDNA was isolated. The DNA and deduced amino acid sequence of the insert are set out in SEQ ID NO: 111 and 112. The full length rat cDNA was named pcs because it does not appear to be the homolog of the human pc3 clone based upon a comparison of the sequences.  
         [0047]    Concurrently, the 0.8 kbp Eco RI-Pst I fragment of partial rat cDNA designated #43 (SEQ ID NO: 113), which was obtained by screening the Stratagene rat brain cDNA library with a probe corresponding to the human pc43 cytoplasmic domain, was used to probe the Stratagene human cDNA library for full length human protocadherin cDNAs. The fragment appears to encode EC-3 through the beginning of EC-6 of clone #43. One partial clone identified encodes a novel human protocadherin named human pc4. The nucleotide sequence and deduced amino acid sequences of the human pc4 clone are set out in SEQ ID NOs: 114 and 115. The amino acid sequence encoded by the pc4 clone appears to begin in the middle of EC-2 of pc4 and continues through the cytoplasmic tail of the protocadherin.  
       EXAMPLE 5  
       [0048]    The full length human cDNAs encoding pc42 and pc43 were expressed in L cells (ATCC CCL 1) using the pRC/RSV expression vector (Invitrogen, San Diego, Calif.). The cDNAs were isolated from the Bluescript SK(+) clones described in Example 2 by digestion with SspI followed by blunt-ending with DNA polymerase and digestion with XbaI (for pc42), or by double digestion with SpeI and EcoRV (for pc43). The pRC/RSV expression vector was digested with HindII, followed by blunt-ending and re-digestion with XbaI for insertion of pc42 sequences, or by digested with XbaI followed by blunt-ending and re-digestion with SpeI for insertion of pc43 sequences. The isolated protocadherin DNAs were ligated into the linearized pRC/RSV vector. The resulting pc42 expression plasmid designated pRC/RSV-pc42 (ATCC 69162) and pc43 expression plasmid designated pRC/RSV-pc43 (ATCC 69163) were purified by CsCl gradient centrifugation and transfected into L cells by a Ca-phosphate method.  
         [0049]    The pc42 and pc43 transfectants were morphologically similar to the parental cells. Northern blot analysis of L cells transfected with pc42 or pc43 DNA sequences showed that the transfected cells expressed mRNAs of a size expected to encode the particular protocadherin.  
       EXAMPLE 6  
       [0050]    Rabbit polyclonal antibodies specific for pc42 and pc43 were generated as well as a mouse monoclonal antibody specific for pc43.  
       Preparation of Polyclonal Antibodies Specific for pc42 and pc43  
       [0051]    DNA sequences encoding portions of the extracellular domain of pc42 and pc43 were each fused to a maltose binding protein-encoding sequence and expressed in bacteria. Specifically, DNAs corresponding to EC4 through EC-7 of pc42 and EC-3 through EC-5 of pc43 were prepared by PCR and subcloned in the correct reading frame into the multicloning site of the pMAL expression vector (New England Biolabs, Beverly, Mass.) which contains sequences encoding maltose binding protein immediately upstream of the multicloning site. The resulting plasmids were then introduced into  E. coli  NM522 cells (Invitrogen, San Diego, Calif.) by a single step transformation method. Expression of the fusion proteins was induced by the addition of IPTG and the fusion proteins were purified from cell extracts by amylose resin affinity chromatography (New England Biolabs) as described by the manufacturer. The fusion proteins were used for the immunization of rabbits without further purification.  
         [0052]    Polyclonal antibodies were prepared in rabbits by immunization at four subcutaneous sites with 500 μg of purified fusion protein in Freund&#39;s complete adjuvant. Subsequent immunizations with 100 μg of the fusion protein were in Freund&#39;s incomplete adjuvant. Immune sera was passed through sepharose coupled to maltose binding protein (New England Biolabs) and polyclonal antibodies were purified from immune sera using Sepharose affinity columns prepared by reaction of the purified fusion protein with CNBr Sepharose (Pharmacia). Reactivity of the polyclonal sera with purified pc42 fusion protein and pc42 transfected cell extracts (described in Example 5) was confirmed.  
       Preparation of Monoclonal Antibodies Specific for pc43  
       [0053]    The pc43 fusion protein (containing the EC-3 through EC-5 subdomains of pc43) was used to generate monoclonal antibodies in mice according to the method of Kennett,  Methods in Enzymol.,  58:345-359 (1978). Briefly, mice were immunized with the pc43 fusion protein (100 μg) at two subcutaneous sites. The spleen from the highest titer mouse was fused to the NS1 myeloma cell line. The resulting hybridoma supernatants were screened in a ELISA assay for reactivity with the pc43 fusion protein and with maltose binding protein. The fusion wells with the highest reactivity to the pc43 extracellular domains were subcloned. The hybridoma cell line designated 38I2C (ATCC HB 11207) produced a IgG 1  subtype monoclonal antibody specific for pc43. Reactivity of the monoclonal antibody produced by hybridoma cell line 38I2C to pc43 was confirmed by immunoblotting the pc43 L cell transfectants described in Example 5. The 38I2C monoclonal antibody is specific for human pc43.  
       EXAMPLE 7  
       [0054]    L cells transfected with DNA sequences encoding pc42 and pc43 as prepared in Example 5 were assayed for expression of the protocadherins by immunoblot and by immunofluorescence microscopy.  
       Immunoblot Analysis  
       [0055]    Cell extracts of pc42 and pc43 transfectants were subjected to SDS-PAGE and then blotted electrophoretically onto a PVDF membrane (Millipore, Bedford, Mass.). The membranes were incubated with 5% skim milk in Tris-buffered saline (TBS) for two hours and then respectively with either pc42 polyclonal sera or pc43 monoclonal antibody for one hour. The membranes were washed three times (for 5 minutes each wash) with TBS containing 0.05% Tween 20 and respectively incubated with alkaline phosphatase-conjugated anti-rabbit IgG antibody or anti-mouse IgG antibody (Promega, Madison, Wis.) in the same buffer for one hour. After washing the membranes with TBS containing 0.05% Tween 20, reactive bands were visualized by using Western Blue solution (Promega).  
         [0056]    Anti-pc42 polyclonal antibodies stained a band of about 170 kDa molecular weight in pc42 transfected cells, but not parental L cells. The pc43-specific monoclonal antibody (38I2C) and polyclonal antibodies stained two adjacent bands of about 150 kDa molecular weight in pc43 transfected cells. The pc43 antibodies did not stain bands in parental L-cells. The molecular weights indicated by the staining of bands by the pc42 and pc43 antibodies are significantly larger than the molecular weights predicted from the deduced amino acid sequences. This discrepancy in molecular weight is common among various cadherin-related proteins and may be attributable to the glycosylation and/or cadherin specific structural properties. The pc42 antibody also stained smaller bands, which may be proteolytic degradation products.  
         [0057]    When transfected cells were trypsinized and cell extracts were prepared, run on SDS/PAGE and immunoblotted with the appropriate antibody, the pc42 and pc43 polypeptides expressed by the transfected cells were found to be highly sensitive to proteolysis and were easily digested by 0.01% trypsin treatment. In contrast to the classic cadherins, however, these proteins were not protected from the digestion in the presence of 1-5mM Ca 2+ .  
       Immunofluorescence Microscopy  
       [0058]    Transfected cells were grown on a cover slip precoated with fibronectin and were fixed with 4% paraformaldehyde for 5 minutes at room temperature or with cold methanol on ice for 10 minutes followed by 4% paraformaldehyde fixation. After washing with TBS, the cells were incubated with TBS containing 1% BSA for 30 minutes and then with anti-pc42 polyclonal antibody or anti-pc43 monoclonal antibody in TBS containing 1% BSA for 1 hour at room temperature. Cover slips were then washed with TBS containing 0.01% BSA and respectively incubated with FITC-conjugated anti-rabbit antibody or anti-mouse antibody (Cappel, Durham, N.C.) for 60 minutes at room temperature. The cells were washed again with TBS containing 0.01% BSA and subjected to fluorescence microscopy. Both pc42-specific and pc43-specific polyclonal antibodies stained the cell periphery of transfected cells expressing the protocadherin proteins, mainly at the cell-cell contact sites. The antibodies did not stain the parent L cells, nor did rabbit preimmune sera stain the pc42 and pc43 transfectants.  
       EXAMPLE 8  
       [0059]    The cell aggregation properties of the transfected L cells expressing protocadherin proteins were examined. Transfected L cells were cultured in Dulbecco&#39;s Modified Eagles Medium (DMEM) (Gibco, Grand Island, N.Y.) supplemented with 10% fetal bovine serum at 37° C. in 5% C 2 . Cells grown near confluence were treated with 0.01% trypsin in the presence of 1 mM EGTA for 25 minutes on a rotary shaker at 37° C. and collected by centrifugation. The cells were washed three times with Ca 2+  free HEPES-buffered saline (HBS) after adding soybean trypsin inhibitor, and were resuspended in HBS containing 1% BSA. The cell aggregation assay [Urushihara et al.,  Dev. Biol.,  70: 206-216 (1979)] was performed by incubating the resuspended cells in a 1:1 mixture of DMEM and HBS containing 1% BSA, 2 mM CaCl 2  and 20 ug/ml of deoxyribonucelease on a rotary shaker at 37° C. for 30 minutes to 6 hours.  
         [0060]    The pc42 and pc43 transfectants did not show any significant cell aggregation activity during periods of incubation less than 1 hour. This is in contrast to the cell aggregation that occurs with classic cadherins in similar experiments (Nagafuchi et al., supra, and Hatta et al., supra). However, prolonged incubation of transfected cells (more than 1-2 hours) resulted in gradual re-aggregation of the cells into small aggregates. Similar results were obtained when single cell suspensions of transfected cells were prepared by trypsin treatment in the presence of Ca 2+ . No re-aggregation was observed under the same conditions when untransfected L cells or L cells transfected with pRC/RSV vector alone were tested. When pc43 transfectants labelled with DiO (Molecular Probes, Eugene, Oreg.) were incubated with unlabelled pc42 transfectants in the cell aggregation assay, aggregation of labelled and unlabelled cells was almost mutually exclusive indicating that protocadherin binding is homophilic.  
         [0061]    In view of the fact that the protocadherin cytoplasmic domains exhibit no apparent homology to cadherin domains, experiments were performed to determine if the difference in cytoplasmic domains could account for the difference in cell aggregation activity observed in cadherin and protocadherin transfectants. The cytoplasmic domain of pc43 was replaced with the cytoplasmic domain of E-cadherin and aggregation of cells transfected with the chimeric construct was analyzed.  
         [0062]    The Bluescript SK(+) clone described in Example 2 which contained the entire coding sequence for pc43 was digested with EcoRV and then partially digested with XbaI to remove the sequence corresponding to the cytoplasmic domain, and the plasmid DNA was purified by agarose gel electrophoresis. The cDNA corresponding to the cytoplasmic domain of mouse E-cadherin was synthesized by PCR using mouse cDNA made from mouse lung mRNA as a template and specific primers corresponding to a region near the N-terminus of the cytoplasmic domain sequence or the region containing the stop codon of mouse E-cadherin (Nagafuchi et al., supra). A XbaI sequence was included to the 5′ end of the upstream primer. The E-cadherin cytoplasmic domain cDNA was then subcloned into the linearized pc43 Bluescript clone. The DNA containing the entire resulting chimeric sequence was cut out with SpeI and EcoRV and was subcloned into the SpeI-blunted XbaI site of the expression vector pRc/RSV vector. Finally, L cells were transfected with the resultant construct by a calcium phosphate method. After screening with G418 for about 10 days, the transfectants were stained with FITC-labeled 38I2C anti-pc43 antibody and subjected to FACS analysis. A portion of highly labeled cells were isolated and cloned. Transfectants showed a morphology similar to that of parental L cells and the expressed protein was localized at the cell periphery using pc43 antibody for immunofluorescence microscopy.  
         [0063]    Cell aggregation activity of the chimeric transfectants was analyzed as follows. The chimeric pc43 transfectants were labeled with DiO for 20 minutes at room temperature. The resultant cells were trypsinized in the presence of 1 mM EGTA and single cell suspension was made. Then, the cells were mixed with unlabeled other type of transfectants and incubated on a rotary shaker for two hours. The results were examined with a fluorescence and a phase contrast microscope apparatus. Antibody inhibition of cell aggregation was examined by incubation of the transfectants in the presence of polyclonal anti-pc43 antibody (100 ng/ml) in the standard assay medium.  
         [0064]    In the cell aggregation assay, the chimeric pc43 transfectants showed clear Ca 2+ -dependent cell aggregation within forty minutes of incubation. Cell aggregation was inhibited by the addition of pc43-specific polyclonal antibody.  
       EXAMPLE 9  
       [0065]    The procedures of Maruyama et al.,  J. Biochem.,  95: 511-519 (1984) were used to determine the calcium binding properties of pc43 by Western blot analysis in the presence or absence of calcium-45. The pc43 fusion protein described in Example 6 containing pc43 subdomains EC-3 through EC-5 was compared to the calcium binding protein calmodulin. Samples of purified pc43 fusion protein were run on SDS/PAGE and electrophoretically transferred to PVDF membrane. Binding of the  45 Ca 2+  to the pc43 fusion protein was detected by autoradiography and was determined to be nearly as efficient as binding of 45 Ca 2+  to calmodulin. In contrast, there was no binding of calcium to purified maltose binding protein lacking the pc43 extracellular domain. The pc43 subdomains EC-3 through EC-5 contain sequences highly homologous to the putative Ca 2±  binding motifs found in E-cadherin. [See, Ringwald et al.,  EMBO J.,  6: 3647-3653 (1987).] 
       EXAMPLE 10  
       [0066]    The expression of mRNA encoding pc42 and pc43 was assayed in various tissues and cell lines by Northern blot.  
         [0067]    Total RNAs were prepared by the guanidium isothiocyanate method and poly(A) + RNAs were isolated using a FastTrack kit (Invitrogen). RNA preparations were electrophoresed in a 0.8% agarose gel under denaturing conditions and transferred onto a nitrocellulose filter using a capillary method. Northern blot analyses were performed according to the method of Thomas,  Proc. Natl. Acad. Sci. USA,  77:5201-5205 (1980). The final wash was in 0.2× standard saline citrate containing 0.1% sodium dodecyl sulfate at 65° C. for 10 minutes.  
       Protocadherin mRNA Expression in Adult Rat Tissues  
       [0068]    Total mRNA preparations of rat tissues including brain, heart, liver, lung, skin, kidney and muscle were separated electrophoretically under denaturing conditions (10 μg mRNA/lane) and transferred onto nitrocellulose filters. The filters were hybridized with  32 P-labelled cDNA fragments MOUSE-326 (which corresponds to EC4 of human pc42) and RAT-218 (which corresponds to EC-5 of human pc43). The mRNAs of both protocadherins were highly expressed in brain. The pc42 probe detected a major band of 7 kb and a minor band of 4 kb in size, possibly representing the products of alternative splicing. The pc43 probe hybridized to a major band of 5 kb in size and with minor bands of smaller sizes.  
       Developmental Expression of Protocadherin mRNA in Rat Brain  
       [0069]    To examine the developmental regulation of mRNA expression of the protocadherins, brain mRNA from rats at embryonic days 17 and 20, neonatal days 5 and 11 and from adult rats was prepared and subjected to Northern blot analysis as described above for other rat tissues. β-actin was used as an internal standard. mRNA levels for pc42 and pc43 proteins increased during embryonic development of the brain as compared with β-actin expression.  
       Protocadherin mRNA Expression in Human Cell Lines  
       [0070]    Several neuronal and glial cell lines (including human SK-N-SH neuroblastoma, human U251 glioma, and mouse Neuro-2a neuroblastoma cell lines) were assayed by Northern blot using  32 P-labelled for expression of pc42 and pc43 mRNA. Human cell lines were probed with HUMAN-42 (which corresponds to EC-4 of human pc42) and HUMAN-43 (which corresponds to EC-5 of human pc43) cDNA fragments while the mouse cell line was probed with MOUSE-326 (which corresponds to EC-4 of human pc42) and RAT-322 (which corresponds to EC-5 of human pc43) cDNA fragments. SK-N-SH human neuroblastoma cells and U251 human glioma cells were found to express pc43 mRNA and Neuro-2a mouse neuroblastoma cells were found to express pc42 mRNA.  
       EXAMPLE 11  
       [0071]    Expression of pc43 protein in various tissues, extracts and cells was assayed by Western blot and immunofluorescence microscopy.  
       Expression in Rat Cardiac Muscle Extracts  
       [0072]    A rat heart non-ionic detergent extract was prepared by freezing a heart in liquid nitrogen after removal, powdering in a mortar and pestle, grinding briefly in a polytron in 0.5% Nonidet P40 in [10 mM PIPES (pH 6.8), 50 mM NaCl, 250 mM NH 4 SO 4 , 300 mM sucrose, 3 mM MgCl 2 ] and microfuging for 15 minutes. Samples were separated by SDS/PAGE and electrophoretically transferred to nitrocellulose (Towbin et al.,  PNAS  76:4350-4354, 1979). Two pc43 protein bands with molecular weights of 150 KDa and 140 KDa were detected with rabbit polyclonal antibodies to pc43 by the immunoblot method described in Example 7.  
       Expression in Tissue Sections and Cells  
       [0073]    To determine the localization of the protocadherins in various tissues, human and rat adult tissues were removed, incubated in 30% sucrose in PBS for 30 minutes at 4° C., embedded in OCT compound (Tissue-Tek, Elkhart, Ind.) in cryomolds and quickly frozen. Six micron sections were cut and placed on glass slides. The slides were washed with PBS and fixed in 3% p-formaldehyde for 5 minutes. To permeablize the tissue sections, the slides were immersed in −20° C. acetone for 10 minutes and air dried. The sections were blocked with 2% goat serum and 1% BSA in PBS for 30 minutes and then incubated with the rabbit anti-pc43 polyclonal antisera for 1 hour at room temperature. The sections were rinsed 3 times in PBS containing 0. 1% BSA and incubated with a biotinylated anti-rabbit (Vector Laboratories, Burlingame, Calif.) in 1% BSA in PBS for 30 minutes. After rinsing 3 times, strepavidin-conjugated with FITC (Vector Laboratories) was added for 30 minutes and again washed 3 times. For co-localization studies, an appropriate primary antibody was used with a TRITC-conjugated secondary antibody.  
         [0074]    A. Muscle  
         [0075]    Immunolocalization of pc43 in rat cardiac muscle shows that pc43 is localized in a repeating pattern which is consistent with pc43 being associated with the sarcomeres. Sarcomeres are repetitive contractile units between the  fascia adherens  in skeletal and cardiac muscle. Co-localization with cytoskeletal proteins shows that pc43 is present at the ends of the sarcomeres in the Z lines which are associated with desmin and the actin-binding protein vinculin, and alpha-actinin. The thin microfilaments of F-actin are associated with the thick myosin filaments between the Z lines. In contrast, N-adherin is localized at the ends of cardiac myocytes at the  fascia adherens  junctions at sites of mycocyte:myocyte contact. The localization of pc43 in cardiac muscle suggests that pc43 may play a role in muscle contraction in the anchoring of the contractile apparatus to the plasma membrane.  
         [0076]    Similar localization for pc43 was observed in rat skeletal muscle. Ultrastructural studies have shown that dystrophin, the gene product lacking in Duchenne muscular dystrophy, is a component of the sarcolemma [Porter et al.,  J. Cell. Biol.,  117:997-1005 (1992)]. The sarcolemma is connected to the contractile apparatus at the M and Z lines where pc43 is localized.  
         [0077]    B. Brain  
         [0078]    Reactivity of anti-pc43 polyclonal antibody and monoclonal antibody 38I2C on frozen sections of rat and human cerebellum, respectively, shows that the major sites of pc43 expression are located in Purkinje cells and the granule cell layer which contains numerous small neurons.  
         [0079]    C. Placenta  
         [0080]    Strong reactivity of monoclonal antibody 38I2C with human syncytiotrophoblasts was also observed in development of the placenta at an early state (5-7 weeks of gestation). Expression appeared to gradually decrease as the stage progressed indicating that pc43 may be involved in the implantation of fertilized eggs into the placenta.  
         [0081]    D. Neuroblastoma and Astrocytoma Cells  
         [0082]    Immunocytochemical localization of pc43 in Sk-N-SH neuroblastoma cells and UW28 astrocytoma cells using anti-pc43 antibodies reveals a punctate cell surface distribution of pc43 and in some cells there is a localization at the tips of extensions of neuronal foot processes. At sites of cell-cell contact of UW28 astrocytoma cells, pc43 is organized in a series of parallel lines. The lines start at the contact site and extend approximately 5 micron. F-actin microfilaments were identified with rhodamine-phalloidin (Molecular Probes, Eugene, Oreg., as described by the manufacturer) showing that the microfilaments in the cell appear to end in the pc43 linear structures which extend from the edge of the cell at sites of cell contact.  
         [0083]    Immunoblotting studies with pc43 specific antibodies show that a protein with a molecular weight of 140 kDa is recognized in human Sk-N-SH neuroblastoma cells and in UW28 astrocytoma cells.  
         [0084]    E. Osteoblasts  
         [0085]    Immunocytochemical localization of pc43 using monoclonal antibody 38I2C in tow human ostogenic sarcoma cell lines [SaOS (ATCC HmB 85) and MG-63 (ATCC CRL 1427)] and in cultures of normal human trabecular osteoblasts [culture system described in Civitelli et al.,  J. Clin. Invest.,  91:1888-1896 (1993)] showed that pc43 is expressed in osteoblasts in a pattern similar to that seen in UW28 astrocytoma cells. At sites of cell-cell contact, pc43 is organized in a series of parallel lines that appear to correspond to the actin stress fibers. In addition, in some cells, pc43 appears to localize at the tips of contacting cell processes. Northern blot analysis provides additional evidence that pc43 is expressed in normal human trabecular osteoblasts. A pc43 specific DNA probe hybridized to a major band of 5 kb in samples of poly-A mRNA isolated from normal human trabecular osteoblasts.  
       EXAMPLE 12  
       [0086]    In situ hybridization experiments using protocadherin specific RNA probes were performed on cryosections of rat tissue.  
         [0087]    Sense and antisense  35 S-riboprobes were made using the standard procedure described by Promega (Madison, Wis.). An approximately 400 bp EcoRI-Xbal fragment of the MOUSE-326 cDNA clone was used as a pc42 specific probe. This fragment encodes the middle of EC-3 to the end of EC-4 of pc42. An approximately 700 bp Smal fragment of the RAT-218 cDNA clone was used as a pc43 specific probe. The fragment encodes the end of EC-3 to the end of EC-5 of pc43.  
         [0088]    Rat adult tissues were harvested and immediately embedded with OCT Compound (Tissue-Tek) in cryomolds and quickly frozen in a bath of 95% ethanol/dry ice. The frozen blocks were stored at −80° C. until cut. Six micron tissue sections were cut using a cryostat (Reichert-Jung, Model #2800 Frigocut N, Leica, Inc., Gitroy, Calif.). Cut tissue sections were stored at −80° C.  
         [0089]    The in situ protocol used was a variation of that described by Angerer et al.,  Methods in Enymology,  152:649-660, (1987). All solutions were treated with diethylpyrocarbonate (DEPC, Sigma, St. Louis, Mo.) to remove RNase contamination. The tissue sections were first fixed in 4% paraformaldehyde at 4° C. for 20 minutes. To remove excess paraformaldehyde and stop the tissue fixation, the slides were washed in PBS (phosphate buffered saline), denatured in a graded series of alcohols (70, 95, 100%) and then dried. To prevent the tissue from detaching from the glass slide during the in situ procedure, the tissue sections were treated in a poly-L-lysine solution (Sigma) at room temperature for 10 minutes. To denature all RNA in the tissue, the sections were placed in a solution of 70% formamide/2×SSC (0.15 M NaCl/0.3 M Na citrate, pH 7.0) at 70° C. for 2 minutes after which they were rinsed in chilled 2×SSC, dehydrated in a graded series of alcohols and then dried. Once dried, the sections were prehybridized in hybridization buffer [50% formamide/50 mM DTT (dithiothrietol)/0.3M NaCl/20 mM Tris, pH 8.0/5 mM EDTA/1×Denhardt&#39;s (0.02% Ficoll Type 400/0.02% polyvinylpyrrolidone/0.02% BSA)/10% Dextran Sulfate] at the final hybridization temperature for approximately 4 hours. After prehybridization, approximately 1×10 6  cpm of the appropriate riboprobe, was added to each section. The sections were generally hybridized at 45° C. overnight (12-16 hours). To insure that the hybridization seen was specific, in some experiments the hybridization stringency was increased by raising the hybridization temperature to 50° C. As both the 45° C. and 50° C. experiments gave comparable results, the standard hybridization temperature used was 45° C.  
         [0090]    To remove excess, nonhybridized probe, the sections were put through a series of washes. The sections were first rinsed in 4×SSC to remove the bulk of the hybridization solution and probe. Next a 15 minute wash in 4×SSC/50 mM DTr was carried out at room temperature. Washes at increased stringencies were also utilized. A 40 minute wash in 50% formamide/2×SSC/50 mM DTr was performed at 60° C. Four final room temperature washes were carried out for 10 minutes each: two in 2×SSC and two in 0.1×SSC. The washed slides were dehydrated in a graded series of alcohols and dried.  
         [0091]    To visualize the hybridized probe, the slides were dipped in Kodak NTB2 nuclear emulsion (International Biotechnology, New Haven, Conn.) which had been diluted 1:1 in dH 2 O. Once dry, the slides were stored at 4° C. in light-tight boxes for the appropriate exposure time. The in situ slides were independently viewed by two persons and scored positive or negative for hybridization signal.  
         [0092]    All in situ hybridization studies were performed on rat tissue. Because results from Northern blot experiments (see Example 9) indicated that both pc42 and pc43 are expressed in adult brain, in situ hybridization studies were carried out to localize the expression of these molecules to specific brain cell types. Hybridization seen in the normal adult rat brain was specific (no background hybridization was seen with the sense probes) and was localized to specific regions in the brain. The overall pattern of expression seen for pc42 and pc43 was very similar, with the major difference being in the level of expression. pc43 appears to be expressed at a lower level than pc42. Both molecules are expressed in the germinal and pyramidal cells of the hippocampus, Purkinjecells of the cerebellum and neurons in grey matter. In addition, pc42 is expressed in glial cells in the white matter but, in contrast to the expression of pc43 in glioma cell lines (as described in Example 9), expression of pc43 in normal glial cells was not observed. In the spinal chord, both protocadherins are expressed in the motor neurons in the gray matter and pc42 is expressed in the glial cells in the white matter.  
         [0093]    When expression of both protocadherin molecules was analyzed in brains and spinal chords from rats having EAE (experimental allergic encephalomyelitis) [Vandenbark et al.,  Cell. Immunol.,  12:85-93 (1974)], the same structures as described above were found to be positive. In addition, expression of pc42 was observed in the leukocytic infiltrates in the EAE tissues. Expression of pc42 in leukocytes was confirmed by in situ hybridization analysis of two leukocytic cell lines, RBL-1 and y3.  
         [0094]    Expression of both protocadherin-42 and -43 was observed in the developing brain of rat embryos at all embryological days tested (E15-E19). In addition protocadherin-43 was observed in the developing rat heart at all embryological days tested (E13-E19). This finding is consistent with the immunohistochemistry results showing protocadherin-43 expression in adult heart.  
         [0095]    To determine possible roles of protocadherins in the development of the nervous system, expression profiles of protocadherin members in developing rat brain and adult rat brain were also examined by in situ hybridization. A series of coronal, sagittal and horizontal sections of rat brains at postnatal days 0, 6, 14, 30 (P0 through P30) and at 3 months (young adult) were hybridized with labelled cRNA probes corresponding to various protocadherins of the invention including pc42, pc43, RAT-212, RAT411, and RAT-418. In developing brain, RAT411 was expressed at high levels in neurons of the olfactory bulb, i.e., mitral cells and periglomerular cells. The expression of RAT-411 mRNA was transient; expression appeared at P0, peaked at P6, diminished by P14, and was undetectable at P30 and in adult brain. In the adult, pc43 mRNA was found to be expressed predominantly in Purkinje cells in the cerebellum. The expression of pc43 mRNA in Purkinje cells was observed from the beginning of Purkinje cell differentiation at around P6. Other protocadherin members were expressed at very low levels in various areas of developing and adult brains. These results indicate that protocadherin members are differentially expressed during the development of the central nervous system, and suggest that RAT-411 and pc43 have specific roles during the development of olfactory bulb neurons and Purkinje cells, respectively.  
       EXAMPLE 13  
       [0096]    Conventional immunoprecipitations using pc43-specific polyclonal antibodies and monoclonal antibody 38I2C were performed to identify proteins that interacted with pc43 in L cell transfectants.  
         [0097]    The pc43 and chimeric pc43 transfectants were metabolically labeled by incubating the cells in Dulbecco&#39;s modified Eagle&#39;s medium containing [ 35 S] methionine (50 uCi/ml) overnight. After washing, the transfectants were lysed with PBS containing Triton ×100 and incubated with anti-pc43 antibody. The immunocomplexes were then collected using protein A-Sepharose beads. The resulting beads were washed five times with a washing buffer (50 mM Tris-HCl, pH 8.0, containing 0.5M NaCl, 0.1% ovalbumin, 0.5% NP-40, 0.5% Triton ×100 and 1 mM EDTA) at room temperature. Protein was separated by SDS-PAGE and subjected to autoradiography.  
         [0098]    The chimeric pc43 co-precipitated with 105 kDa and a 95 kDa bands that are likely to correspond to α- and β-catenins, respectively, because anti-α-catenin and anti-β-catenin antibodies stained comparable bands. Pc43, on the other hand, co-precipitated with a 120 kDa band.  
         [0099]    While the present invention has been described in terms of specific methods and compositions, it is understood that variations and modifications will occur to those skilled in the art. Therefore, only such limitations as appear in the claims should be placed on the invention.   
     
       
       
         1 
         
           
             
115 
 
           
           
             
               17 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               DNA  
             
              1 

AARSSNNTNG AYTRYGA                                                    17 

 
           
           
             
               17 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               DNA  
             
              2 

TTRCTRTTRC GNGGNNN                                                    17 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              3 

AAGGGAGTGG ACTTTGAGGA GCAGCCTGAG CTTAGTCTCA TCCTCACGGC TTTGGATGGA     60 

GGGACTCCAT CCAGGTCTGG GACTGCATTG GTTCAAGTGG AAGTCATAGA TGCCAATGAC    120 

AACGCACCGT A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              4 

Lys Gly Val Asp Phe Glu Glu Gln Pro Glu Leu Ser Leu Ile Leu Thr 
1               5                   10                  15 

Ala Leu Asp Gly Gly Thr Pro Ser Arg Ser Gly Thr Ala Leu Val Gln 
            20                  25                  30 

Val Glu Val Ile Asp Ala Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              5 

AAACGCATGG ATTTCGAGGA GTCTTCCTCC TACCAGATCT ATGTGCAAGC TACTGACCGG     60 

GGACCAGTAC CCATGGCGGG TCATTGCAAG GTGTTGGTGG ACATTATAGA TGTGAACGAC    120 

AACGCACCTA A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              6 

Lys Ala Met Asp Phe Glu Glu Ser Ser Ser Tyr Gln Ile Tyr Val Gln 
1               5                   10                  15 

Ala Thr Asp Arg Gly Pro Val Pro Met Ala Gly His Cys Lys Val Leu 
            20                  25                  30 

Val Asp Ile Ile Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              7 

AAGCGACTGG ACTTTGAGAC CCTGCAGACC TTCGAGTTCA GCGTGGGTGC CACAGACCAT     60 

GGCTCCCCCT CGCTCCGCAG TCAGGCTCTG GTGCGCGTGG TGGTGCTGGA CCACAATGAC    120 

AATGCCCCCA A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              8 

Lys Arg Leu Asp Phe Glu Thr Leu Gln Thr Phe Glu Phe Ser Val Gly 
1               5                   10                  15 

Ala Thr Asp His Gly Ser Pro Ser Leu Arg Ser Gln Ala Leu Val Arg 
            20                  25                  30 

Val Val Val Leu Asp His Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              9 

AAGGGCCTGG ATTACGAGGC ACTGCAGTCC TTCGAGTTCT ACGTGGGCGC TACAGATGGA     60 

GGCTCACCCG CGCTCAGCAG CCAGACTCTG GTGCGGATGG TGGTGCTGGA TGACAACGAC    120 

AACGCCCCTA A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              10 

Lys Gly Leu Asp Tyr Glu Ala Leu Gln Ser Phe Glu Phe Tyr Val Gly 
1               5                   10                  15 

Ala Thr Asp Gly Gly Ser Pro Ala Leu Ser Ser Gln Thr Leu Val Arg 
            20                  25                  30 

Met Val Val Leu Asp Asp Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              11 

AAGGCGTTTG ATTTTGAGGA TCAGAGAGAG TTCCAGCTAA CCGCTCATAT AAACGACGGA     60 

GGTACCCCGG TTTTGGCCAC CAACATCAGC GTGAACATAT TTGTTACTGA CCGCAATGAC    120 

AACGCCCCGC A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              12 

Lys Ala Phe Asp Phe Glu Asp Gln Arg Glu Phe Gln Leu Thr Ala His 
1               5                   10                  15 

Ile Asn Asp Gly Gly Thr Pro Val Leu Ala Thr Asn Ile Ser Val Asn 
            20                  25                  30 

Ile Phe Val Thr Asp Arg Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              13 

AAGGCGGTGG ATTACGAAAT CACCAAGTCC TATGAGATAG ATGTTCAAGC CCAAGATCTG     60 

GGTCCCAATT CTATTCCTGC TCATTGCAAA ATTATAATTA AGGTCGTGGA TGTCAACGAC    120 

AACGCTCCCA A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              14 

Lys Ala Val Asp Tyr Glu Ile Thr Lys Ser Tyr Glu Ile Asp Val Gln 
1               5                   10                  15 

Ala Gln Asp Leu Gly Pro Asn Ser Ile Pro Ala His Cys Lys Ile Ile 
            20                  25                  30 

Ile Lys Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               135 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              15 

TATGACCATG ATTACGAGAC AACCAAAGAA TATACACTGC GGATCCGGGC CCAGGATGGT     60 

GGCCGGACTC CACTTTCCAA CGTCTCCGGT CTAGTAACCG TGCAGGTCCT AGACATCAAC    120 

GACAATGCCC CCCCA                                                     135 

 
           
           
             
               44 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              16 

Tyr Asp His Asp Tyr Glu Thr Thr Lys Glu Tyr Thr Leu Arg Ile Arg 
1               5                   10                  15 

Ala Gln Asp Gly Gly Arg Thr Pro Leu Ser Asn Val Ser Gly Leu Val 
            20                  25                  30 

Thr Val Gln Val Leu Asp Ile Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               129 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              17 

GGGGGGTCGA TTACGAGGAG AACGGCATGT TAGAGATCGA CGTGCAGGCC AGAGACCTAG     60 

GACCTAACCC AATTCCAGCC CATTGCAAGG TCACAGTCAA GCTCATCGAC CGCAATGATA    120 

ACGCCCCCA                                                            129 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              18 

Arg Gly Val Asp Tyr Glu Glu Asn Gly Met Leu Glu Ile Asp Val Gln 
1               5                   10                  15 

Ala Arg Asp Leu Gly Pro Asn Pro Ile Pro Ala His Cys Lys Val Thr 
            20                  25                  30 

Val Lys Leu Ile Asp Arg Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              19 

AAGGGGTTGG ACTACGAAGA CACCAAACTC CATGAGATTT ACATCCAGGC CAAAGACAAA     60 

GGTGCCAATC CGGAAGGAGC GCATTGCAAA GTACTGGTAG AGGTTGTGGA CGTTAACGAC    120 

AATGCCCCTC A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              20 

Lys Gly Leu Asp Tyr Glu Asp Thr Lys Leu His Glu Ile Tyr Ile Gln 
1               5                   10                  15 

Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys Lys Val Leu 
            20                  25                  30 

Val Glu Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              21 

AAGGGTTTGG ACTTTGAGCA AGTAGATGTC TACAAAATCC GCGTTGACGC GACGGACAAA     60 

GGACACCCTC CGATGGCAGG CCATTGCACT GTTTTAGTGA GGGTATTGGA TGAAAACGAC    120 

AATGCGCCTC T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              22 

Lys Gly Leu Asp Phe Glu Gln Val Asp Val Tyr Lys Ile Arg Val Asp 
1               5                   10                  15 

Ala Thr Asp Lys Gly His Pro Pro Met Ala Gly His Cys Thr Val Leu 
            20                  25                  30 

Val Arg Val Leu Asp Glu Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               134 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              23 

AAGGGTATAG ACTTCGAGCA GATCAAGGAC TTCAGCTTTC AAGTGGAAGC CCGGGACGCC     60 

GGCAGTCCCC AGGCGCTGTC CGGCAACTGC ACTGTCAACA TCTTGATAGT GGATCAGAAC    120 

GACAACGCCC CTAA                                                      134 

 
           
           
             
               44 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              24 

Lys Gly Ile Asp Phe Glu Gln Ile Lys Asp Phe Ser Phe Gln Val Glu 
1               5                   10                  15 

Ala Arg Asp Ala Gly Ser Pro Gln Ala Leu Ala Gly Asn Thr Thr Val 
            20                  25                  30 

Asn Ile Leu Ile Val Asp Gln Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               134 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              25 

AAGCCGTTCG ACTATGAGCA AACCGCCAAC ACGCTGGCAC AGATTGACGC CGTGCTGGAA     60 

AAACAGGGCA GCAATAAATC GAGCATTCTG GATGCCACCA TTTTCCTGGC CGATAAAAAC    120 

GACAATGCGC CAGA                                                      134 

 
           
           
             
               44 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              26 

Lys Pro Phe Asp Tyr Glu Gln Thr Ala Asn Thr Leu Ala Gln Ile Asp 
1               5                   10                  15 

Ala Val Leu Glu Lys Gln Gly Ser Asn Lys Ser Ser Ile Leu Asp Ala 
            20                  25                  30 

Thr Ile Phe Leu Ala Asp Lys Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              27 

AAGCGGCTGG ATTTCGAACA GTTCCAGCAG CACAAGCTGC TCGTAAGGGC TGTTGATGGA     60 

GGAATGCCGC CACTGAGCAG CGATGTGGTC GTCACTGTGG ATGTCACCGA CCTCAACGAT    120 

AACGCGCCCT A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              28 

Lys Arg Leu Asp Phe Glu Gln Phe Gln Gln His Lys Leu Leu Val Arg 
1               5                   10                  15 

Ala Val Asp Gly Gly Met Pro Pro Leu Ser Ser Asp Val Val Val Thr 
            20                  25                  30 

Val Asp Val Thr Asp Leu Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              29 

AAGGGGATAG ACTTTGAGAG TGAGAATTAC TATGAATTTG ATGTGCGGGC TCGCGATGGG     60 

GGTTCTCCAG CCATGGAGCA ACATTGCAGC CTTCGAGTGG ATCTGCTGGA CGTAAATGAC    120 

AACGCCCCAC T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              30 

Lys Gly Ile Asp Phe Glu Ser Glu Asn Tyr Tyr Glu Phe Asp Val Arg 
1               5                   10                  15 

Ala Arg Asp Gly Gly Ser Pro Ala Met Glu Gln His Cys Ser Leu Arg 
            20                  25                  30 

Val Asp Leu Leu Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              31 

AAGGCATTGG ACTTTGAGGC CCGGCGACTG TATTCGCTGA CAGTTCAGGC CACGGACCGA     60 

GGCGTGCCCT CGCTCACCGG GCGTGCCGAA GCGCTTATCC AGCTGCTAGA TGTCAACGAC    120 

AACGCACCCA T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              32 

Lys Ala Leu Asp Phe Glu Ala Arg Arg Leu Tyr Ser Leu Thr Val Gln 
1               5                   10                  15 

Ala Thr Asp Arg Gly Val Pro Ser Leu Thr Gly Arg Ala Glu Ala Leu 
            20                  25                  30 

Ile Gln Leu Leu Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               125 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              33 

AAGCCAATTG ATTACGAGGC AACTCCATAC TATAACATGG AAATTGTAGC CACAGACAGC     60 

GGAGGTCTTT CGGGAAAATG CACTGTGTCT ATACAGGTGG TGGATGTGAA CGACAACGCC    120 

CCCAA                                                                125 

 
           
           
             
               41 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              34 

Lys Pro Ile Asp Tyr Glu Ala Thr Pro Tyr Tyr Asn Met Glu Ile Val 
1               5                   10                  15 

Ala Thr Asp Ser Gly Gly Leu Ser Gly Lys Cys Thr Val Ser Ile Gln 
            20                  25                  30 

Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               446 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              35 

AAGCGGGTAG ACTTCGAAAT GTGCAAAAGA TTTTACCTTG TGGTGGAAGC TAAAGACGGA     60 

GGCACCCCAG CCCTCAGCAC GGCAGCCACT GTCAGCATCG ACCTCACAGA TGTGAATGAT    120 

AACCCTCCTC GGTTCAGCCA AGATGTCTAC AGTGCTGTCA TCAGTGAGGA TGCCTTAGAG    180 

GGGGACTCTG TCATTCTGCT GATAGCAGAA GATGTGGATA GCAAGCCTAA TGGACAGATT    240 

CGGTTTTCCA TCGTGGGTGG AGATAGGGAC AATGAATTTG CTGTCGATCC AATCTTGGGA    300 

CTTGTGAAAG TTAAGAAGAA ACTGGACCGG GAGCGGGTGT CAGGATACTC CCTGCTCATC    360 

CAGGCAGTAG ATAGTGGCAT TCCTGCAATG TCCTCAACGA CAACTGTCAA CATTGATATT    420 

TCTGATGTGA ACGACAACGC CCCCCT                                         446 

 
           
           
             
               148 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              36 

Lys Arg Val Asp Phe Glu Met Cys Lys Arg Phe Tyr Leu Val Val Glu 
1               5                   10                  15 

Ala Lys Asp Gly Gly Thr Pro Ala Leu Ser Thr Ala Ala Thr Val Ser 
            20                  25                  30 

Ile Asp Leu Thr Asp Val Asn Asp Asn Pro Pro Arg Phe Ser Gln Asp 
        35                  40                  45 

Val Tyr Asp Ala Val Ile Ser Glu Asp Ala Leu Glu Gly Asp Ser Val 
    50                  55                  60 

Ile Leu Leu Ile Ala Glu Asp Val Asp Ser Lys Pro Asn Gly Gln Ile 
65                  70                  75                  80 

Arg Phe Ser Ile Val Gly Gly Asp Arg Asp Asn Glu Phe Ala Val Asp 
                85                  90                  95 

Pro Ile Leu Gly Leu Val Lys Val Lys Lys Lys Leu Asp Arg Glu Arg 
            100                 105                 110 

Val Ser Gly Tyr Ser Leu Leu Ile Gln Ala Val Asp Ser Gly Ile Pro 
        115                 120                 125 

Ala Met Ser Ser Thr Thr Thr Val Asn Ile Asp Ile Ser Asp Val Asn 
    130                 135                 140 

Asp Asn Ala Pro 
145 

 
           
           
             
               440 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              37 

AAGGGGGTTG ATTATGAGAC AAACCCACGG CTACGACTGG TGCTACAGGC AGAGAGTGGA     60 

GGAGCCTTTG CTTTCTCGGT GCTGACCCTG ACCCTTCAAG ATGCCAATGA CAATGCTCCC    120 

CGTTTCCTGC AGCCTCACTA CGTGGCTTTC CTGCCAGAGT CCCGACCCTT GGAAGGGCCC    180 

CTGCTGCAGG TGGAAGCAGA CGACCTGGAT CAAGGCTCTG GAGGACAGAT CTCCTACAGT    240 

CTGGCTGCAT CCCAGCCAGC ACGGGGCTTG TTCCATGTAG ACCCAGCCAC AGGCACTATC    300 

ACTACCACAG CCATCCTGGA CCGGGAAATC TGGGCTGAAA CACGGCTGGT ACTGATGGCC    360 

ACAGACAGAG GAAGCCCAGC ATTGGTGGGC TCAGCTACCC TGACAGTGAT GGTCATCGAT    420 

ACCAACGACA ATGCTCCCCT                                                440 

 
           
           
             
               146 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              38 

Lys Gly Val Asp Tyr Glu Thr Asn Pro Arg Leu Arg Leu Val Leu Gln 
1               5                   10                  15 

Ala Glu Ser Gly Gly Ala Phe Ala Phe Ser Val Leu Thr Leu Thr Leu 
            20                  25                  30 

Gln Asp Ala Asn Asp Asn Ala Pro Arg Phe Leu Gln Pro His Tyr Val 
        35                  40                  45 

Ala Phe Leu Pro Glu Ser Arg Pro Leu Glu Gly Pro Leu Leu Gln Val 
    50                  55                  60 

Glu Ala Asn Asp Leu Asp Gln Gly Ser Gly Gly Gln Ile Ser Tyr Ser 
65                  70                  75                  80 

Leu Ala Ala Ser Gln Pro Ala Arg Gly Leu Phe His Val Asp Pro Ala 
                85                  90                  95 

Thr Gly Thr Ile Thr Thr Thr Ala Ile Leu Asp Arg Glu Ile Trp Ala 
            100                 105                 110 

Glu Thr Arg Leu Val Leu Met Ala Thr Asp Arg Gly Ser Pro Ala Leu 
        115                 120                 125 

Val Gly Ser Ala Thr Leu Thr Val Met Val Ile Asp Thr Asn Asp Asn 
    130                 135                 140 

Ala Pro 
145 

 
           
           
             
               124 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              39 

AAGGTCTCGA TTATGAGGCA ACTCCATATT ATAACGTGGA AATTGTAGCC ACAGATGGTG     60 

GGGGCCTTTC AGGAAAATGC ACTGTGGCTA TAGAAGTGGT GGATGTGAAC GACGGCGCTC    120 

CAAT                                                                 124 

 
           
           
             
               41 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              40 

Lys Gly Leu Asp Tyr Glu Ala Thr Pro Tyr Tyr Asn Val Glu Ile Val 
1               5                   10                  15 

Ala Thr Asp Gly Gly Ala Phe Asp Glu Asn Cys Thr Val Ala Ile Glu 
            20                  25                  30 

Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               8 amino acids  
               amino acid  
               single  
               linear  
             
             
               DNA  
             
              41 

Asp Xaa Asn Glu Xaa Pro Xaa Phe 
1               5 

 
           
           
             
               8 amino acids  
               amino acid  
               single  
               linear  
             
             
               DNA  
             
              42 

Asp Xaa Asp Glu Xaa Pro Xaa Phe 
1               5 

 
           
           
             
               9 amino acids  
               amino acid  
               single  
               linear  
             
             
               DNA  
             
              43 

Asp Xaa Asn Asp Asn Xaa Pro Xaa Phe 
1               5 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              44 

AAGCGGATGG ATTTTGAAGA CACCAAACTC CATGAGATTT ACATCCAGGC CAAAGACAAA     60 

GGTGCCAATC CCGAAGGAGC GCATTGCAAA GTACTTGTAG AGGTTGTAGA CGTAAACGAC    120 

AACGCCCCAG T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              45 

Leu Arg Met Asp Phe Glu Asp Thr Lys Leu His Glu Ile Tyr Ile Gln 
1               5                   10                  15 

Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys Lys Val Leu 
            20                  25                  30 

Val Glu Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              46 

AAGGCTTTGG ATTACGAGGA TCAGAGAGAG TTCCAACTAA CAGCTCATAT AAACGACGGA     60 

GGTACCCCAG TCTTAGCCAC CAACATCAGC GTGAACGTAT TTGTTACTGA CCGCAATGAT    120 

AACGCCCCCT A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              47 

Lys Ala Leu Asp Tyr Glu Asp Gln Arg Glu Phe Gln Leu Thr Ala Hi 
1               5                   10                  15 

Ile Asn Asp Gly Gly Thr Pro Val Leu Ala Thr Asn Ile Ser Val As 
            20                  25                  30 

Val Phe Val Thr Asp Arg Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              48 

AAGCGCTTGG ACTACGAGGA GAGTAACAAT TATGAAATTC ACGTGGATGC TACAGATAAA     60 

GGATACCCAC CTATGGTTGC TCACTGCACC GTACTCGTGG GAATCTTGGA TGAAAATGAC    120 

AACGCACCCA T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              49 

Lys Arg Leu Asp Tyr Glu Glu Ser Asn Asn Tyr Glu Ile His Val As 
1               5                   10                  15 

Ala Thr Asp Lys Gly Tyr Pro Pro Met Val Ala His Cys Thr Val Le 
            20                  25                  30 

Val Gly Ile Leu Asp Glu Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              50 

AAACCGGTGG ACTACGAGAA AGTCAAAGAC TATACCATCG AGATCGTGGC TGTGGATTCC     60 

GGCAACCCTC CACTCTCTAG CACCAACTCC CTCAAGGTGC AGGTGGTAGA CGTCAACGAT    120 

AACGCCCCTC T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              51 

Lys Pro Val Asp Tyr Glu Lys Val Lys Asp Tyr Thr Ile Glu Ile Val 
1               5                   10                  15 

Ala Val Asp Ser Gly Asn Pro Pro Leu Ser Ser Thr Asn Ser Leu Lys 
            20                  25                  30 

Val Gln Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              52 

AAGCCTTTTG ATTTCGAGGA CACCAAACTC CATGAGATTT ACATCCAGGC CAAAGACAAG     60 

GGCGCCAATC CCGAAGGAGC ACATTGCAAA GTGTTGGTGG AGGTTGTGGA TGTGAACGAC    120 

AATGCCCCTC A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              53 

Lys Pro Phe Asp Phe Glu Asp Thr Lys Leu His Glu Ile Tyr Ile Gln 
1               5                   10                  15 

Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys Lys Val Leu 
            20                  25                  30 

Val Glu Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               122 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              54 

AAAGGTGTCG ATTACGAGGT GAGTCCACGG CTGCGACTGG TGCTGCAGGC AGAGAGTCGA     60 

GGAGCCTTTG CCTTCACTGT GCTGACCCTG ACCCTGCAAG ATGCCAACGA CAACGCCCCG    120 

AG                                                                   122 

 
           
           
             
               40 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              55 

Lys Gly Val Asp Tyr Glu Val Ser Pro Arg Leu Arg Leu Val Leu Gln 
1               5                   10                  15 

Ala Glu Ser Arg Gly Ala Phe Ala Phe Thr Val Leu Thr Leu Thr Leu 
            20                  25                  30 

Gln Asp Ala Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              56 

AAAGGGATTG ATTACGAGCA GTTGAGAGAC CTACAGCTGT GGGTGACAGC CAGCGACAGC     60 

GGGGACCCGC CTCTTAGCAG CAACGTGTCA CTGAGCCTGT TTGTGCTGGA CCAGAACGAC    120 

AACGCCCCCC T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              57 

Lys Gly Ile Asp Tyr Glu Gln Leu Arg Asp Leu Gln Leu Trp Val Th 
1               5                   10                  15 

Ala Ser Asp Ser Gly Asp Pro Pro Leu Ser Ser Asn Val Ser Leu Se 
            20                  25                  30 

Leu Phe Val Leu Asp Gln Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               125 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              58 

AAGGCGGTCG ATTTTGAGCG CACATCCTCT TATCAACTCA TCATTCAGGC CACCAATATG     60 

GCAGGAATGG CTTCCAATGC TACAGTCAAT ATTCAGATTG TTGATGAAAA CGACAACGCC    120 

CCCCA                                                                125 

 
           
           
             
               41 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              59 

Lys Ala Val Asp Phe Glu Arg Thr Ser Ser Tyr Gln Leu Ile Ile Gln 
1               5                   10                  15 

Ala Thr Asn Met Ala Gly Met Ala Ser Asn Ala Thr Val Asn Ile Gln 
            20                  25                  30 

Ile Val Asp Glu Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              60 

AAACGGCTAG ACTTTGAAAA GATACAAAAA TATGTTGTAT GGATAGAGGC CAGAGATGGT     60 

GGTTTCCCTC CTTTCTCCTC TTACGAGAAA CTTGATATAA CAGTATTAGA TGTCAACGAT    120 

AACGCGCCTA A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              61 

Lys Arg Leu Asp Phe Glu Lys Ile Gln Lys Tyr Val Val Trp Ile Glu 
1               5                   10                  15 

Ala Arg Asp Gly Gly Phe Pro Pro Phe Ser Ser Tyr Glu Lys Leu Asp 
            20                  25                  30 

Ile Thr Val Leu Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              62 

AAGGGGATCG ATTATGAGAA GGTCAAAGAC TACACCATTG AGATTGTGGC TGTGGACTCT     60 

GGCAACCCCC CACTCTCCAG CACTAACTCC CTCAAGGTGC AGGTGGTGGA CGTCAATGAC    120 

AACGCACCGT G                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              63 

Lys Gly Ile Asp Tyr Glu Lys Val Lys Asp Tyr Thr Ile Glu Ile Val 
1               5                   10                  15 

Ala Val Asp Ser Gly Asn Pro Pro Leu Ser Ser Thr Asn Ser Leu Lys 
            20                  25                  30 

Val Gln Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              64 

AAGGGACTCG ACTACGAGGA TCGGCGGGAA TTTGAATTAA CAGCTCATAT CAGCGATGGG     60 

GGCACCCCGG TCCTAGCCAC CAACATCAGC GTGAACATAT TTGTCACTGA TCGCAACGAT    120 

AATGCCCCCG T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              65 

Lys Gly Leu Asp Tyr Glu Asp Arg Arg Glu Phe Glu Leu Thr Ala His 
1               5                   10                  15 

Ile Ser Asp Gly Gly Thr Pro Val Leu Ala Thr Asn Ile Ser Val Asn 
            20                  25                  30 

Ile Phe Val Thr Asp Arg Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               470 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              66 

AAGGGTTTGG ACTACGAGAC CACACAGGCC TACCAGCTCA CGGTCAACGC CACAGATCAA     60 

GACAACACCA GGCCTCTGTC CACCCTGGCC AACTTGGCCA TCATCATCAC AGATGTCCAG    120 

GACATGGACC CCATCTTCAT CAACCTGCCT TACAGCACCA ACATCTACGA GCATTCTCCT    180 

CCGGGCACGA CGGTGCGCAT CATCACCGCC ATAGACCAGG ATCAAGGACG TCCCCGGGGC    240 

ATTGGCTACA CCATCGTTTC AGGGAATACC AACAGCATCT TTGCCCTGGA CTACATCAGC    300 

GGAGTGCTGA CCTTGAATGG CCTGCTGGAC CGGGAGAACC CCCTGTACAG CCATGGCTTC    360 

ATCCTGACTG TGAAGGGCAC GGAGCTGAAC GATGACCGCA CCCCATCTGA CGCTACAGTC    420 

ACCACGACCT TCAATATCCT GGTTATTGAC ATCAACGACA ACGCCCCACT               470 

 
           
           
             
               156 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              67 

Lys Gly Leu Asp Tyr Glu Thr Thr Gln Ala Tyr Gln Leu Thr Val Asn 
1               5                   10                  15 

Ala Thr Asp Gln Asp Asn Thr Arg Pro Leu Ser Thr Leu Ala Asn Leu 
            20                  25                  30 

Ala Ile Ile Ile Thr Asp Val Gln Asp Met Asp Pro Ile Phe Ile Asn 
        35                  40                  45 

Leu Pro Tyr Ser Thr Asn Ile Tyr Glu His Ser Pro Pro Gly Thr Thr 
    50                  55                  60 

Val Arg Ile Ile Thr Ala Ile Asp Gln Asp Gln Gly Arg Pro Arg Gly 
65                  70                  75                  80 

Ile Gly Tyr Thr Ile Val Ser Gly Asn Thr Asn Ser Ile Phe Ala Leu 
                85                  90                  95 

Asp Tyr Ile Ser Gly Val Leu Thr Leu Asn Gly Leu Leu Asp Arg Glu 
            100                 105                 110 

Asn Pro Leu Tyr Ser Gly Gly Phe Ile Leu Thr Val Lys Gly Thr Glu 
        115                 120                 125 

Leu Asn Asp Asp Arg Thr Pro Ser Asp Ala Thr Val Thr Thr Thr Phe 
    130                 135                 140 

Asn Ile Leu Val Ile Asp Ile Asn Asp Asn Ala Pro 
145                 150                 155 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              68 

AAGGGGGTCG ATTACGAGGT ACTACAGGCC TTTGAGTTCC ACGTGAGCGC CACAGACCGA     60 

GGCTCACCGG GGCTCAGCAG CCAGGCTCTG GTGCGCGTGG TGGTGCTGGA CGACAATGAC    120 

AACGCTCCCG T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              69 

Lys Gly Val Asp Tyr Glu Val Leu Gln Ala Phe Glu Phe His Val Ser 
1               5                   10                  15 

Ala Thr Asp Arg Gly Ser Pro Gly Leu Ser Ser Gln Ala Leu Val Arg 
            20                  25                  30 

Val Val Val Leu Asp Asp Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              70 

AAGGGGCTGG ATTATGAGCA GTTCCAGACC CTACAACTGG GAGTGACCGC TAGTGACAGT     60 

GGAAACCCAC CATTAAGAAG CAATATTTCA CTGACCCTTT TCGTGCTGGA CCAGAATGAT    120 

AACGCCCCAA A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              71 

Lys Gly Leu Asp Tyr Glu Gln Phe Gln Thr Leu Gln Leu Gly Val Thr 
1               5                   10                  15 

Ala Ser Asp Ser Gly Asn Pro Pro Leu Arg Ser Asn Ile Ser Leu Thr 
            20                  25                  30 

Leu Phe Val Leu Asp Gln Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              72 

AAGCGGGTTG ATTACGAGGA TGTCCAGAAA TACTCGCTGA GCATTAAGGC CCAGGATGGG     60 

CGGCCCCCGC TCATCAATTC TTCAGGGGTG GTGTCTGTGC AGGTGCTGGA TGTCAACGAC    120 

AATGCCCCGG A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               peptide  
             
              73 

Lys Arg Val Asp Tyr Glu Asp Val Gln Lys Tyr Ser Leu Ser Ile Lys 
1               5                   10                  15 

Ala Gln Asp Gly Arg Pro Pro Leu Ile Asn Ser Ser Gly Val Val Ser 
            20                  25                  30 

Val Gln Val Leu Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               125 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              74 

AAACCGGTAG ACTTTGAGCT ACAGCAGTTC TATGAAGTAG CTGTGGTGGC TTGGAACTCT     60 

GAGGGATTTC ATGTCAAAAG GGTCATTAAA GTGCAACTTT TAGATGACAA CGACAATGCC    120 

CCGAT                                                                125 

 
           
           
             
               41 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              75 

Lys Pro Val Asp Phe Glu Leu Gln Gln Phe Tyr Glu Val Ala Val Val 
1               5                   10                  15 

Ala Trp Asn Ser Glu Gly Phe His Val Lys Arg Val Ile Lys Val Gln 
            20                  25                  30 

Leu Leu Asp Asp Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               125 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              76 

AAGGGATTAG ATTTTGAAAC TTTGCCCATT TACACATTGA TAATACAAGG AACTAACATG     60 

GCTGGTTTGT CCACTAATAC AACGGTTCTA GTTCACTTGC AGGATGAGAA TGATAACGCC    120 

CCAAA                                                                125 

 
           
           
             
               41 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              77 

Lys Gly Leu Asp Phe Glu Thr Leu Pro Ile Tyr Thr Leu Ile Ile Gln 
1               5                   10                  15 

Gly Thr Asn Met Ala Gly Leu Ser Thr Asn Thr Thr Val Leu Val His 
            20                  25                  30 

Leu Gln Asp Glu Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               134 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              78 

AAGCGGGCGG ATTTCGAGGC GATCCGGGAG TACAGTCTGA GGATCAAAGC GCAGGACGGG     60 

GGGCGGCCTC CCCTCAGCAA CACCACGGGC ATGGTCACAG TGCAGGTCGT GGACGTCAAT    120 

GACAACGCAC CCCT                                                      134 

 
           
           
             
               44 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              79 

Lys Arg Ala Asp Phe Glu Ala Ile Arg Glu Tyr Ser Leu Arg Ile Lys 
1               5                   10                  15 

Ala Gln Asp Gly Gly Arg Pro Pro Leu Ser Asn Thr Thr Gly Met Val 
            20                  25                  30 

Thr Val Gln Val Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              80 

AAGCGGTTGG ATTACGAAAA GGCATCGGAA TATGAAATCT ATGTTCAAGC CGCTGACAAA     60 

GGCGCTGTCC CTATGGCTGG CCATTGCAAA GTGTTGCTGG AGATCGTGGA TGTCAACGAC    120 

AACGCCCCCT T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              81 

Lys Arg Leu Asp Tyr Glu Lys Ala Ser Glu Tyr Glu Ile Tyr Val Gln 
1               5                   10                  15 

Ala Ala Asp Lys Gly Ala Val Pro Met Ala Gly His Cys Lys Val Leu 
            20                  25                  30 

Leu Glu Ile Val Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              82 

AAGGGGATCG ATTATGAGGA TCAGGTCTCT TACACATTAG CAGTAACAGC ACATGACTAT     60 

GGCATCCCTC AAAAATCAGA CACTACCTAT TTGGAAATCT TAGTAATTGA TGTTAACGAC    120 

AACGCGCCCC A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              83 

Lys Gly Ile Asp Tyr Glu Asp Gln Val Ser Tyr Thr Leu Ala Val Thr 
1               5                   10                  15 

Ala His Asp Tyr Gly Ile Pro Gln Lys Ser Asp Thr Thr Tyr Leu Glu 
            20                  25                  30 

Ile Leu Val Ile Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              84 

AAAGGGTTAG ATTTCGAGGG CACTAAAGAT TCAGCGTTTA AAATAGTGGC AGCTGACACA     60 

GGGAAGCCCA GCCTCAACCA GACAGCCCTG GTGAGAGTAG AGCTGGAGGA TGAGAACGAC    120 

AACGCCCCAA T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              85 

Lys Gly Leu Asp Phe Glu Gly Thr Lys Asp Ser Ala Phe Lys Ile Val 
1               5                   10                  15 

Ala Ala Asp Thr Gly Lys Pro Ser Leu Asn Gln Thr Ala Leu Val Arg 
            20                  25                  30 

Val Glu Leu Glu Asp Glu Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               130 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              86 

AAGGGTGTGG ATTTTGAAAG TGTGCGTAGC TACAGGCTGG TTATTCGTGC TCAAGATGGA     60 

GGCAGCCCCT CCAGAAGTAA CACCACCCAG CTCTTGGTCA ACGTCATCGA TCGAATGACA    120 

ATGCGCCGCT                                                           130 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              87 

Lys Gly Val Asp Phe Glu Ser Val Arg Ser Tyr Arg Leu Val Ile Arg 
1               5                   10                  15 

Ala Gln Asp Gly Gly Ser Pro Ser Arg Ser Asn Thr Thr Gln Leu Leu 
            20                  25                  30 

Val Asn Val Ile Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              88 

AAGGGTGTGG ACTTCGAGCT GACACATCTG TATGAGATTT GGATTGAGGC TGCCGATGGA     60 

GACACGCCAA GTCTGCGTAG TGTAACTCTT ATAACGCTCA ACGTAACGGA TGCCAATGAC    120 

AATGCTCCCA A                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              89 

Lys Gly Val Asp Phe Glu Leu Thr His Leu Tyr Glu Ile Trp Ile Glu 
1               5                   10                  15 

Ala Ala Asp Gly Asp Thr Pro Ser Leu Arg Ser Val Thr Leu Ile Thr 
            20                  25                  30 

Leu Asn Val Thr Asp Ala Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               441 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              90 

CAAGGCGTTT GATTTTGAAG AGACAAGTAG ATATGTGTTG AGTGTGGAAG CTAAGGATGG     60 

AGGAGTACAC ACAGCTCACT GTAATGTTCA AATAGAAATT GTTGACGAGA ATGACAATGC    120 

CCCAGAGGTG ACATTCATGT CCTTCTCTAA CCAGATTCCA GAGGATTCAG ACCTTGGAAC    180 

TGTAATAGCC CTCATAAAAG TGCGAGACAA GGATTCTGGG CAAAATGGCA TGGTGACATG    240 

CTATACTCAG GAAGAAGTTC CTTTCAAATT AGAATCCACC TCGAAGAATT ATTACAAGCT    300 

GGTGATTGCT GGAGCCCTAA ACCGGGAGCA GACAGCAGAC TACAACGTCA CAATCATAGC    360 

CACCGACAAG GGCAAACCAG CCCTTTCCTC CAGGACAAGC ATCACCCTGC ACATCTCCGA    420 

CATCAACGAT AATGCCCCCG T                                              441 

 
           
           
             
               146 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              91 

Lys Ala Phe Asp Phe Glu Glu Thr Ser Arg Tyr Val Leu Ser Val Glu 
1               5                   10                  15 

Ala Lys Asp Gly Gly Val His Thr Ala His Cys Asn Val Gln Ile Glu 
            20                  25                  30 

Ile Val Asp Glu Asn Asp Asn Ala Pro Glu Val Thr Phe Met Ser Phe 
        35                  40                  45 

Ser Asn Gln Ile Pro Glu Asp Ser Asp Leu Gly Thr Val Ile Ala Leu 
    50                  55                  60 

Ile Lys Val Arg Asp Lys Asp Ser Gly Gln Asn Gly Met Val Thr Cys 
65                  70                  75                  80 

Tyr Thr Gln Glu Glu Val Pro Phe Lys Leu Glu Ser Thr Ser Lys Asn 
                85                  90                  95 

Tyr Tyr Lys Leu Val Ile Ala Gly Ala Leu Asn Arg Glu Gln Thr Ala 
            100                 105                 110 

Asp Tyr Asn Val Thr Ile Ile Ala Thr Asp Lys Gly Lys Pro Ala Leu 
        115                 120                 125 

Ser Ser Arg Thr Ser Ile Thr Leu His Ile Ser Asp Ile Asn Asp Asn 
    130                 135                 140 

Ala Pro 
145 

 
           
           
             
               131 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              92 

AAGCGAGTGG ATTACGAGGC CACTCGGAAT TATAAGCTGA GAGTTAAGGC TACTGATCTT     60 

GGGATTCCAC CGAGATCTTC TAACATGACA CTGTTCATTC ATGTCCTTGA TGTTAACGAC    120 

AACGCTCCCT T                                                         131 

 
           
           
             
               43 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              93 

Lys Arg Val Asp Tyr Glu Ala Thr Arg Asn Tyr Lys Leu Arg Val Lys 
1               5                   10                  15 

Ala Thr Asp Leu Gly Ile Pro Pro Arg Ser Ser Asn Met Thr Leu Phe 
            20                  25                  30 

Ile His Val Leu Asp Val Asn Asp Asn Ala Pro 
        35                  40 

 
           
           
             
               4104 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
             
               CDS  
                495..3572 

 
             
              94 

CCTCTATTCG ACATTCTCTT TGGATTGTTT TGCTATAACT TGAAATTTGG GATGTCACAA     60 

ACGAAACTGT CATCTGTTTC CGCCAAACTG TGGTTCTGCT AATCTCCCAG GCTGGCAGCA    120 

TTGGAGACTT GCTGACTTCT TTCATCCCCC ACTCTTTTCA CCTGAAATTC CTTTCCTTGA    180 

TTTTGCTCTA AGTCCTATGC TTCAGTCAGG GGCCAACCAA ATCTCACTGC CTCCTTTTTA    240 

TCATGAAGCC TTTGATCACT GATAGTTCTT TTTATATCTT GAAAAATCAC CCTTCCCAGT    300 

ACAGTTAATA TTTAGTATCT CTACTCATCT TGGCACTTAC TCACAGCTCC ATAATTCAGT    360 

CGTTTTCGTA CCTCTTCATG GTGATGGGGA GCCCTTTGGA GGTGGTGACT GTGCTTTATA    420 

CTCCTCATGA TGCTTCACAT GTGGCAGGCG TGGAGTGCCC GGAGGCGGCC CTCCTGATTC    480 

TGGGGCCTCC CAGG ATG GAG CCC CTG AGG CAC AGC CCA GGC CCT GGG GGG      530 
                Met Glu Pro Leu Arg His Ser Pro Gly Pro Gly Gly 
                  1               5                  10 

CAA CGG CTA CTG CTG CCC TCC ATG CTG CTA GCA CTG CTG CTC CTG CTG      578 
Gln Arg Leu Leu Leu Pro Ser Met Leu Leu Ala Leu Leu Leu Leu Leu 
         15                  20                  25 

GCT CCA TCC CCA GGC CAC GCC ACT CGG GTA GTG TAC AAG GTG CCG GAG      626 
Ala Pro Ser Pro Gly His Ala Thr Arg Val Val Tyr Lys Val Pro Glu 
     30                  35                  40 

GAA CAG CCA CCC AAC ACC CTC ATT GGG AGC CTC GCA GCC GAC TAT GGT      674 
Glu Gln Pro Pro Asn Thr Leu Ile Gly Ser Leu Ala Ala Asp Tyr Gly 
 45                  50                  55                  60 

TTT CCA GAT GTG GGG CAC CTG TAC AAG CTA GAG GTG GGT GCC CCG TAC      722 
Phe Pro Asp Val Gly His Leu Tyr Lys Leu Glu Val Gly Ala Pro Tyr 
                 65                  70                  75 

CTT CGC GTG GAT GGC AAG ACA GGT GAC ATT TTC ACC ACC GAG ACC TCC      770 
Leu Arg Val Asp Gly Lys Thr Gly Asp Ile Phe Thr Thr Glu Thr Ser 
             80                  85                  90 

ATC GAC CGT GAG GGG CTC CGT GAA TGC CAG AAC CAG CTC CCT GGT GAT      818 
Ile Asp Arg Glu Gly Leu Arg Glu Cys Gln Asn Gln Leu Pro Gly Asp 
         95                 100                 105 

CCC TGC ATC CTG GAG TTT GAG GTA TCT ATC ACA GAC CTC GTG CAG AAT      866 
Pro Cys Ile Leu Glu Phe Glu Val Ser Ile Thr Asp Leu Val Gln Asn 
    110                 115                 120 

GCG AGC CCC CGG CTG CTA GAG GGC CAG ATA GAA GTA CAA GAC ATC AAT      914 
Ala Ser Pro Arg Leu Leu Glu Gly Gln Ile Glu Val Gln Asp Ile Asn 
125                 130                 135                 140 

GAC AAC ACA CCC AAC TTC GCC TCA CCA GTC ATC ACT CTG GCC ATC CCT      962 
Asp Asn Thr Pro Asn Phe Ala Ser Pro Val Ile Thr Leu Ala Ile Pro 
                145                 150                 155 

GAG AAC ACC AAC ATC GGC TCA CTC TTC CCC ATC CCG CTG GCT TCA GAC     1010 
Glu Asn Thr Asn Ile Gly Ser Leu Phe Pro Ile Pro Leu Ala Ser Asp 
            160                 165                 170 

CGT GAT GCT GGT CCC AAC GGT GTG GCA TCC TAT GAG CTG CAG GTG GCA     1058 
Arg Asp Ala Gly Pro Asn Gly Val Ala Ser Tyr Glu Leu Gln Val Ala 
        175                 180                 185 

GAG GAC CAG GAG GAG AAG CAA CCA CAG CTC ATT GTG ATG GGC AAC CTG     1106 
Glu Asp Gln Glu Glu Lys Gln Pro Gln Leu Ile Val Met Gly Asn Leu 
    190                 195                 200 

GAC CGT GAG CGC TGG GAC TCC TAT GAC CTC ACC ATC AAG GTG CAG GAT     1154 
Asp Arg Glu Arg Trp Asp Ser Tyr Asp Leu Thr Ile Lys Val Gln Asp 
205                 210                 215                 220 

GGC GGC AGC CCC CCA CGC GCC ACG AGT GCC CTG CTG CGT GTC ACC GTG     1202 
Gly Gly Ser Pro Pro Arg Ala Thr Ser Ala Leu Leu Arg Val Thr Val 
                225                 230                 235 

CTT GAC ACC AAT GAC AAC GCC CCC AAG TTT GAG CGG CCC TCC TAT GAG     1250 
Leu Asp Thr Asn Asp Asn Ala Pro Lys Phe Glu Arg Pro Ser Tyr Glu 
            240                 245                 250 

GCC GAA CTA TCT GAG AAT AGC CCC ATA GGC CAC TCG GTC ATC CAG GTG     1298 
Ala Glu Leu Ser Glu Asn Ser Pro Ile Gly His Ser Val Ile Gln Val 
        255                 260                 265 

AAG GCC AAT GAC TCA GAC CAA GGT GCC AAT GCA GAA ATC GAA TAC ACA     1346 
Lys Ala Asn Asp Ser Asp Gln Gly Ala Asn Ala Glu Ile Glu Tyr Thr 
    270                 275                 280 

TTC CAC CAG GCG CCC GAA GTT GTG AGG CGT CTT CTT CGA CTG GAC AGG     1394 
Phe His Gln Ala Pro Glu Val Val Arg Arg Leu Leu Arg Leu Asp Arg 
285                 290                 295                 300 

AAC ACT GGA CTT ATC ACT GTT CAG GGC CCG GTG GAC CGT GAG GAC CTA     1442 
Asn Thr Gly Leu Ile Thr Val Gln Gly Pro Val Asp Arg Glu Asp Leu 
                305                 310                 315 

AGC ACC CTG CGC TTC TCA GTG CTT GCT AAG GAC CGA GGC ACC AAC CCC     1490 
Ser Thr Leu Arg Phe Ser Val Leu Ala Lys Asp Arg Gly Thr Asn Pro 
            320                 325                 330 

AAG AGT GCC CGT GCC CAG GTG GTT GTG ACC GTG AAG GAC ATG AAT GAC     1538 
Lys Ser Ala Arg Ala Gln Val Val Val Thr Val Lys Asp Met Asn Asp 
        335                 340                 345 

AAT GCC CCC ACC ATT GAG ATC CGG GGC ATA GGG CTA GTG ACT CAT CAA     1586 
Asn Ala Pro Thr Ile Glu Ile Arg Gly Ile Gly Leu Val Thr His Gln 
    350                 355                 360 

GAT GGG ATG GCT AAC ATC TCA GAG GAT GTG GCA GAG GAG ACA GCT GTG     1634 
Asp Gly Met Ala Asn Ile Ser Glu Asp Val Ala Glu Glu Thr Ala Val 
365                 370                 375                 380 

GCC CTG GTG CAG GTG TCT GAC CGA GAT GAG GGA GAG AAT GCA GCT GTC     1682 
Ala Leu Val Gln Val Ser Asp Arg Asp Glu Gly Glu Asn Ala Ala Val 
                385                 390                 395 

ACC TGT GTG GTG GCA GGT GAT GTG CCC TTC CAG CTG CGC CAG GCC AGT     1730 
Thr Cys Val Val Ala Gly Asp Val Pro Phe Gln Leu Arg Gln Ala Ser 
            400                 405                 410 

GAG ACA GGC AGT GAC AGC AAG AAG AAG TAT TTC CTG CAG ACT ACC ACC     1778 
Glu Thr Gly Ser Asp Ser Lys Lys Lys Tyr Phe Leu Gln Thr Thr Thr 
        415                 420                 425 

CCG CTA GAC TAC GAG AAG GTC AAA GAC TAC ACC ATT GAG ATT GTG GCT     1826 
Pro Leu Asp Tyr Glu Lys Val Lys Asp Tyr Thr Ile Glu Ile Val Ala 
    430                 435                 440 

GTG GAC TCT GGC AAC CCC CCA CTC TCC AGC ACT AAC TCC CTC AAG GTG     1874 
Val Asp Ser Gly Asn Pro Pro Leu Ser Ser Thr Asn Ser Leu Lys Val 
445                 450                 455                 460 

CAG GTG GTG GAC GTC AAT GAC AAC GCA CCT GTC TTC ACT CAG AGT GTC     1922 
Gln Val Val Asp Val Asn Asp Asn Ala Pro Val Phe Thr Gln Ser Val 
                465                 470                 475 

ACT GAG GTC GCC TTC CCG GAA AAC AAC AAG CCT GGT GAA GTG ATT GCT     1970 
Thr Glu Val Ala Phe Pro Glu Asn Asn Lys Pro Gly Glu Val Ile Ala 
            480                 485                 490 

GAG ATC ACT GCC AGT GAT GCT GAC TCT GGC TCT AAT GCT GAG CTG GTT     2018 
Glu Ile Thr Ala Ser Asp Ala Asp Ser Gly Ser Asn Ala Glu Leu Val 
        495                 500                 505 

TAC TCT CTG GAG CCT GAG CCG GCT GCT AAG GGC CTC TTC ACC ATC TCA     2066 
Tyr Ser Leu Glu Pro Glu Pro Ala Ala Lys Gly Leu Phe Thr Ile Ser 
    510                 515                 520 

CCC GAG ACT GGA GAG ATC CAG GTG AAG ACA TCT CTG GAT CGG GAA CAG     2114 
Pro Glu Thr Gly Glu Ile Gln Val Lys Thr Ser Leu Asp Arg Glu Gln 
525                 530                 535                 540 

CGG GAG AGC TAT GAG TTG AAG GTG GTG GCA GCT GAC CGG GGC AGT CCT     2162 
Arg Glu Ser Tyr Glu Leu Lys Val Val Ala Ala Asp Arg Gly Ser Pro 
                545                 550                 555 

AGC CTC CAG GGC ACA GCC ACT GTC CTT GTC AAT GTG CTG GAC TGC AAT     2210 
Ser Leu Gln Gly Thr Ala Thr Val Leu Val Asn Val Leu Asp Cys Asn 
            560                 565                 570 

GAC AAT GAC CCC AAA TTT ATG CTG AGT GGC TAC AAC TTC TCA GTG ATG     2258 
Asp Asn Asp Pro Lys Phe Met Leu Ser Gly Tyr Asn Phe Ser Val Met 
        575                 580                 585 

GAG AAC ATG CCA GCA CTG AGT CCA GTG GGC ATG GTG ACT GTC ATT GAT     2306 
Glu Asn Met Pro Ala Leu Ser Pro Val Gly Met Val Thr Val Ile Asp 
    590                 595                 600 

GGA GAC AAG GGG GAG AAT GCC CAG GTG CAG CTC TCA GTG GAG CAG GAC     2354 
Gly Asp Lys Gly Glu Asn Ala Gln Val Gln Leu Ser Val Glu Gln Asp 
605                 610                 615                 620 

AAC GGT GAC TTT GTT ATC CAG AAT GGC ACA GGC ACC ATC CTA TCC AGC     2402 
Asn Gly Asp Phe Val Ile Gln Asn Gly Thr Gly Thr Ile Leu Ser Ser 
                625                 630                 635 

CTG AGC TTT GAT CGA GAG CAA CAA AGC ACC TAC ACC TTC CAG CTG AAG     2450 
Leu Ser Phe Asp Arg Glu Gln Gln Ser Thr Tyr Thr Phe Gln Leu Lys 
            640                 645                 650 

GCA GTG GAT GGT GGC GTC CCA CCT CGC TCA GCT TAC GTT GGT GTC ACC     2498 
Ala Val Asp Gly Gly Val Pro Pro Arg Ser Ala Tyr Val Gly Val Thr 
        655                 660                 665 

ATC AAT GTG CTG GAC GAG AAT GAC AAC GCA CCC TAT ATC ACT GCC CCT     2546 
Ile Asn Val Leu Asp Glu Asn Asp Asn Ala Pro Tyr Ile Thr Ala Pro 
    670                 675                 680 

TCT AAC ACC TCT CAC AAG CTG CTG ACC CCC CAG ACA CGT CTT GGT GAG     2594 
Ser Asn Thr Ser His Lys Leu Leu Thr Pro Gln Thr Arg Leu Gly Glu 
685                 690                 695                 700 

ACG GTC AGC CAG GTG GCA GCC GAG GAC TTT GAC TCT GGT GTC AAT GCC     2642 
Thr Val Ser Gln Val Ala Ala Glu Asp Phe Asp Ser Gly Val Asn Ala 
                705                 710                 715 

GAG CTG ATC TAC AGC ATT GCA GGT GGC AAC CCT TAT GGA CTC TTC CAG     2690 
Glu Leu Ile Tyr Ser Ile Ala Gly Gly Asn Pro Tyr Gly Leu Phe Gln 
            720                 725                 730 

ATT GGG TCA CAT TCA GGT GCC ATC ACC CTG GAG AAG GAG ATT GAG CGG     2738 
Ile Gly Ser His Ser Gly Ala Ile Thr Leu Glu Lys Glu Ile Glu Arg 
        735                 740                 745 

CGC CAC CAT GGG CTA CAC CGC CTG GTG GTG AAG GTC AGT GAC CGC GGC     2786 
Arg His His Gly Leu His Arg Leu Val Val Lys Val Ser Asp Arg Gly 
    750                 755                 760 

AAG CCC CCA CGC TAT GGC ACA GCC TTG GTC CAT CTT TAT GTC AAT GAG     2834 
Lys Pro Pro Arg Tyr Gly Thr Ala Leu Val His Leu Tyr Val Asn Glu 
765                 770                 775                 780 

ACT CTG GCC AAC CGC ACG CTG CTG GAG ACC CTC CTG GGC CAC AGC CTG     2882 
Thr Leu Ala Asn Arg Thr Leu Leu Glu Thr Leu Leu Gly His Ser Leu 
                785                 790                 795 

GAC ACG CCG CTG GAT ATT GAC ATT GCT GGG GAT CCA GAA TAT GAG CGC     2930 
Asp Thr Pro Leu Asp Ile Asp Ile Ala Gly Asp Pro Glu Tyr Glu Arg 
            800                 805                 810 

TCC AAG CAG CGT GGC AAC ATT CTC TTT GGT GTG GTG GCT GGT GTG GTG     2978 
Ser Lys Gln Arg Gly Asn Ile Leu Phe Gly Val Val Ala Gly Val Val 
        815                 820                 825 

GCC GTG GCC TTG CTC ATC GCC CTG GCG GTT CTT GTG CGC TAC TGC AGA     3026 
Ala Val Ala Leu Leu Ile Ala Leu Ala Val Leu Val Arg Tyr Cys Arg 
    830                 835                 840 

CAG CGG GAG GCC AAA AGT GGT TAC CAG GCT GGT AAG AAG GAG ACC AAG     3074 
Gln Arg Glu Ala Lys Ser Gly Tyr Gln Ala Gly Lys Lys Glu Thr Lys 
845                 850                 855                 860 

GAC CTG TAT GCC CCC AAG CCC AGT GGC AAG GCC TCC AAG GGA AAC AAA     3122 
Asp Leu Tyr Ala Pro Lys Pro Ser Gly Lys Ala Ser Lys Gly Asn Lys 
                865                 870                 875 

AGC AAA GGC AAG AAG AGC AAG TCC CCA AAG CCC GTG AAG CCA GTG GAG     3170 
Ser Lys Gly Lys Lys Ser Lys Ser Pro Lys Pro Val Lys Pro Val Glu 
            880                 885                 890 

GAC GAG GAT GAG GCC GGG CTG CAG AAG TCC CTC AAG TTC AAC CTG ATG     3218 
Asp Glu Asp Glu Ala Gly Leu Gln Lys Ser Leu Lys Phe Asn Leu Met 
        895                 900                 905 

AGC GAT GCC CCT GGG GAC AGT CCC CGC ATC CAC CTG CCC CTC AAC TAC     3266 
Ser Asp Ala Pro Gly Asp Ser Pro Arg Ile His Leu Pro Leu Asn Tyr 
    910                 915                 920 

CCA CCA GGC AGC CCT GAC CTG GGC CGC CAC TAT CGC TCT AAC TCC CCA     3314 
Pro Pro Gly Ser Pro Asp Leu Gly Arg His Tyr Arg Ser Asn Ser Pro 
925                 930                 935                 940 

CTG CCT TCC ATC CAG CTG CAG CCC CAG TCA CCC TCA GCC TCC AAG AAG     3362 
Leu Pro Ser Ile Gln Leu Gln Pro Gln Ser Pro Ser Ala Ser Lys Lys 
                945                 950                 955 

CAC CAG GTG GTA CAG GAC CTG CCA CCT GCA AAC ACA TTC GTG GGC ACC     3410 
His Gln Val Val Gln Asp Leu Pro Pro Ala Asn Thr Phe Val Gly Thr 
            960                 965                 970 

GGG GAC ACC ACG TCC ACG GGC TCT GAG CAG TAC TCC GAC TAC AGC TAC     3458 
Gly Asp Thr Thr Ser Thr Gly Ser Glu Gln Tyr Ser Asp Tyr Ser Tyr 
        975                 980                 985 

CGC ACC AAC CCC CCC AAA TAC CCC AGC AAG CAG GTA GGC CAG CCC TTT     3506 
Arg Thr Asn Pro Pro Lys Tyr Pro Ser Lys Gln Val Gly Gln Pro Phe 
    990                 995                 1000 

CAG CTC AGC ACA CCC CAG CCC CTA CCC CAC CCC TAC CAC GGA GCC ATC     3554 
Gln Leu Ser Thr Pro Gln Pro Leu Pro His Pro Tyr His Gly Ala Ile 
1005                1010                1015                1020 

TGG ACC GAG GTG TGG GAG TGATGGAGCA GGTTTACTGT GCCTGCCCGT            3602 
Trp Thr Glu Val Trp Glu 
                1025 

GTTGGGGGCC AGCCTGAGCC AGCAGTGGGA GGTGGGGCCT TAGTGCCTCA CCGGGCACAC   3662 

GGATTAGGCT GAGTGAAGAT TAAGGGAGGG TGTGCTCTGT GGTCTCCTCC CTGCCCTCTC   3722 

CCCACTGGGG AGAGACCTGT GATTTGCCAA GTCCCTGGAC CCTGGACCAG CTACTGGGCC   3782 

TTATGGGTTG GGGGTGGTAG GCAGGTGAGC GTAAGTGGGG AGGGAAATGG GTAAGAAGTC   3842 

TACTCCAAAC CTAGGTCTCT ATGTCAGACC AGACCTAGGT GCTTCTCTAG GAGGGAAACA   3902 

GGGAGACCTG GGGTCCTGTG GATAACTGAG TGGGGAGTCT GCCAGGGGAG GGCACCTTCC   3962 

CATTGTGCCT TCTGTGTGTA TTGTGCATTA ACCTCTTCCT CACCACTAGG CTTCTGGGGC   4022 

TGGGTCCCAC ATGCCCTTGA CCCTGACAAT AAAGTTCTCT ATTTTTGGAA AAAAAAAAAA   4082 

AAAAAAAAAA AAAAAAAAAA AA                                            4104 

 
           
           
             
               1026 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              95 

Met Glu Pro Leu Arg His Ser Pro Gly Pro Gly Gly Gln Arg Leu Leu 
  1               5                  10                  15 

Leu Pro Ser Met Leu Leu Ala Leu Leu Leu Leu Leu Ala Pro Ser Pro 
             20                  25                  30 

Gly His Ala Thr Arg Val Val Tyr Lys Val Pro Glu Glu Gln Pro Pro 
         35                  40                  45 

Asn Thr Leu Ile Gly Ser Leu Ala Ala Asp Tyr Gly Phe Pro Asp Val 
     50                  55                  60 

Gly His Leu Tyr Lys Leu Glu Val Gly Ala Pro Tyr Leu Arg Val Asp 
 65                  70                  75                  80 

Gly Lys Thr Gly Asp Ile Phe Thr Thr Glu Thr Ser Ile Asp Arg Glu 
                 85                  90                  95 

Gly Leu Arg Glu Cys Gln Asn Gln Leu Pro Gly Asp Pro Cys Ile Leu 
            100                 105                 110 

Glu Phe Glu Val Ser Ile Thr Asp Leu Val Gln Asn Ala Ser Pro Arg 
        115                 120                 125 

Leu Leu Glu Gly Gln Ile Glu Val Gln Asp Ile Asn Asp Asn Thr Pro 
    130                 135                 140 

Asn Phe Ala Ser Pro Val Ile Thr Leu Ala Ile Pro Glu Asn Thr Asn 
145                 150                 155                 160 

Ile Gly Ser Leu Phe Pro Ile Pro Leu Ala Ser Asp Arg Asp Ala Gly 
                165                 170                 175 

Pro Asn Gly Val Ala Ser Tyr Glu Leu Gln Val Ala Glu Asp Gln Glu 
            180                 185                 190 

Glu Lys Gln Pro Gln Leu Ile Val Met Gly Asn Leu Asp Arg Glu Arg 
        195                 200                 205 

Trp Asp Ser Tyr Asp Leu Thr Ile Lys Val Gln Asp Gly Gly Ser Pro 
    210                 215                 220 

Pro Arg Ala Thr Ser Ala Leu Leu Arg Val Thr Val Leu Asp Thr Asn 
225                 230                 235                 240 

Asp Asn Ala Pro Lys Phe Glu Arg Pro Ser Tyr Glu Ala Glu Leu Ser 
                245                 250                 255 

Glu Asn Ser Pro Ile Gly His Ser Val Ile Gln Val Lys Ala Asn Asp 
            260                 265                 270 

Ser Asp Gln Gly Ala Asn Ala Glu Ile Glu Tyr Thr Phe His Gln Ala 
        275                 280                 285 

Pro Glu Val Val Arg Arg Leu Leu Arg Leu Asp Arg Asn Thr Gly Leu 
    290                 295                 300 

Ile Thr Val Gln Gly Pro Val Asp Arg Glu Asp Leu Ser Thr Leu Arg 
305                 310                 315                 320 

Phe Ser Val Leu Ala Lys Asp Arg Gly Thr Asn Pro Lys Ser Ala Arg 
                325                 330                 335 

Ala Gln Val Val Val Thr Val Lys Asp Met Asn Asp Asn Ala Pro Thr 
            340                 345                 350 

Ile Glu Ile Arg Gly Ile Gly Leu Val Thr His Gln Asp Gly Met Ala 
        355                 360                 365 

Asn Ile Ser Glu Asp Val Ala Glu Glu Thr Ala Val Ala Leu Val Gln 
    370                 375                 380 

Val Ser Asp Arg Asp Glu Gly Glu Asn Ala Ala Val Thr Cys Val Val 
385                 390                 395                 400 

Ala Gly Asp Val Pro Phe Gln Leu Arg Gln Ala Ser Glu Thr Gly Ser 
                405                 410                 415 

Asp Ser Lys Lys Lys Tyr Phe Leu Gln Thr Thr Thr Pro Leu Asp Tyr 
            420                 425                 430 

Glu Lys Val Lys Asp Tyr Thr Ile Glu Ile Val Ala Val Asp Ser Gly 
        435                 440                 445 

Asn Pro Pro Leu Ser Ser Thr Asn Ser Leu Lys Val Gln Val Val Asp 
    450                 455                 460 

Val Asn Asp Asn Ala Pro Val Phe Thr Gln Ser Val Thr Glu Val Ala 
465                 470                 475                 480 

Phe Pro Glu Asn Asn Lys Pro Gly Glu Val Ile Ala Glu Ile Thr Ala 
                485                 490                 495 

Ser Asp Ala Asp Ser Gly Ser Asn Ala Glu Leu Val Tyr Ser Leu Glu 
            500                 505                 510 

Pro Glu Pro Ala Ala Lys Gly Leu Phe Thr Ile Ser Pro Glu Thr Gly 
        515                 520                 525 

Glu Ile Gln Val Lys Thr Ser Leu Asp Arg Glu Gln Arg Glu Ser Tyr 
    530                 535                 540 

Glu Leu Lys Val Val Ala Ala Asp Arg Gly Ser Pro Ser Leu Gln Gly 
545                 550                 555                 560 

Thr Ala Thr Val Leu Val Asn Val Leu Asp Cys Asn Asp Asn Asp Pro 
                565                 570                 575 

Lys Phe Met Leu Ser Gly Tyr Asn Phe Ser Val Met Glu Asn Met Pro 
            580                 585                 590 

Ala Leu Ser Pro Val Gly Met Val Thr Val Ile Asp Gly Asp Lys Gly 
        595                 600                 605 

Glu Asn Ala Gln Val Gln Leu Ser Val Glu Gln Asp Asn Gly Asp Phe 
    610                 615                 620 

Val Ile Gln Asn Gly Thr Gly Thr Ile Leu Ser Ser Leu Ser Phe Asp 
625                 630                 635                 640 

Arg Glu Gln Gln Ser Thr Tyr Thr Phe Gln Leu Lys Ala Val Asp Gly 
                645                 650                 655 

Gly Val Pro Pro Arg Ser Ala Tyr Val Gly Val Thr Ile Asn Val Leu 
            660                 665                 670 

Asp Glu Asn Asp Asn Ala Pro Tyr Ile Thr Ala Pro Ser Asn Thr Ser 
        675                 680                 685 

His Lys Leu Leu Thr Pro Gln Thr Arg Leu Gly Glu Thr Val Ser Gln 
    690                 695                 700 

Val Ala Ala Glu Asp Phe Asp Ser Gly Val Asn Ala Glu Leu Ile Tyr 
705                 710                 715                 720 

Ser Ile Ala Gly Gly Asn Pro Tyr Gly Leu Phe Gln Ile Gly Ser His 
                725                 730                 735 

Ser Gly Ala Ile Thr Leu Glu Lys Glu Ile Glu Arg Arg His His Gly 
            740                 745                 750 

Leu His Arg Leu Val Val Lys Val Ser Asp Arg Gly Lys Pro Pro Arg 
        755                 760                 765 

Tyr Gly Thr Ala Leu Val His Leu Tyr Val Asn Glu Thr Leu Ala Asn 
    770                 775                 780 

Arg Thr Leu Leu Glu Thr Leu Leu Gly His Ser Leu Asp Thr Pro Leu 
785                 790                 795                 800 

Asp Ile Asp Ile Ala Gly Asp Pro Glu Tyr Glu Arg Ser Lys Gln Arg 
                805                 810                 815 

Gly Asn Ile Leu Phe Gly Val Val Ala Gly Val Val Ala Val Ala Leu 
            820                 825                 830 

Leu Ile Ala Leu Ala Val Leu Val Arg Tyr Cys Arg Gln Arg Glu Ala 
        835                 840                 845 

Lys Ser Gly Tyr Gln Ala Gly Lys Lys Glu Thr Lys Asp Leu Tyr Ala 
    850                 855                 860 

Pro Lys Pro Ser Gly Lys Ala Ser Lys Gly Asn Lys Ser Lys Gly Lys 
865                 870                 875                 880 

Lys Ser Lys Ser Pro Lys Pro Val Lys Pro Val Glu Asp Glu Asp Glu 
                885                 890                 895 

Ala Gly Leu Gln Lys Ser Leu Lys Phe Asn Leu Met Ser Asp Ala Pro 
            900                 905                 910 

Gly Asp Ser Pro Arg Ile His Leu Pro Leu Asn Tyr Pro Pro Gly Ser 
        915                 920                 925 

Pro Asp Leu Gly Arg His Tyr Arg Ser Asn Ser Pro Leu Pro Ser Ile 
    930                 935                 940 

Gln Leu Gln Pro Gln Ser Pro Ser Ala Ser Lys Lys His Gln Val Val 
945                 950                 955                 960 

Gln Asp Leu Pro Pro Ala Asn Thr Phe Val Gly Thr Gly Asp Thr Thr 
                965                 970                 975 

Ser Thr Gly Ser Glu Gln Tyr Ser Asp Tyr Ser Tyr Arg Thr Asn Pro 
            980                 985                 990 

Pro Lys Tyr Pro Ser Lys Gln Val Gly Gln Pro Phe Gln Leu Ser Thr 
        995                 1000                1005 

Pro Gln Pro Leu Pro His Pro Tyr His Gly Ala Ile Trp Thr Glu Val 
    1010                1015                1020 

Trp Glu 
1025 

 
           
           
             
               4705 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
             
               CDS  
                115..2827 

 
             
              96 

CGAAAGCCAT GTCGGACTCG TCGCCCAGCG CCCAAGCGCT AACCCGCTGA AAGTTTCTCA     60 

GCGAAATCTC AGGGACGATC TGGACCCCGC TGAGAGGAAC TGCTTTTGAG TGAG ATG      117 
                                                            Met 
                                                              1 

GTC CCA GAG GCC TGG AGG AGC GGA CTG GTA AGC ACC GGG AGG GTA GTG      165 
Val Pro Glu Ala Trp Arg Ser Gly Leu Val Ser Thr Gly Arg Val Val 
              5                  10                  15 

GGA GTT TTG CTT CTG CTT GGT GCC TTG AAC AAG GCT TCC ACG GTC ATT      213 
Gly Val Leu Leu Leu Leu Gly Ala Leu Asn Lys Ala Ser Thr Val Ile 
         20                  25                  30 

CAC TAT GAG ATC CCG GAG GAA AGA GAG AAG GGT TTC GCT GTG GGC AAC      261 
His Tyr Glu Ile Pro Glu Glu Arg Glu Lys Gly Phe Ala Val Gly Asn 
     35                  40                  45 

GTG GTC GCG AAC CTT GGT TTG GAT CTC GGT AGC CTC TCA GCC CGC AGG      309 
Val Val Ala Asn Leu Gly Leu Asp Leu Gly Ser Leu Ser Ala Arg Arg 
 50                  55                  60                  65 

TTC CCG GTG GTG TCT GGA GCT AGC CGA AGA TTC TTT GAG GTG AAC CGG      357 
Phe Pro Val Val Ser Gly Ala Ser Arg Arg Phe Phe Glu Val Asn Arg 
                 70                  75                  80 

GAG ACC GGA GAG ATG TTT GTG AAC GAC CGT CTG GAT CGA GAG GAG CTG      405 
Glu Thr Gly Glu Met Phe Val Asn Asp Arg Leu Asp Arg Glu Glu Leu 
             85                  90                  95 

TGT GGG ACA CTG CCC TCT TGC ACT GTA ACT CTG GAG TTG GTA GTG GAG      453 
Cys Gly Thr Leu Pro Ser Cys Thr Val Thr Leu Glu Leu Val Val Glu 
        100                 105                 110 

AAC CCG CTG GAG CTG TTC AGC GTG GAA GTG GTG ATC CAG GAC ATC AAC      501 
Asn Pro Leu Glu Leu Phe Ser Val Glu Val Val Ile Gln Asp Ile Asn 
    115                 120                 125 

GAC AAC AAT CCT GCT TTC CCT ACC CAG GAA ATG AAA TTG GAG ATT AGC      549 
Asp Asn Asn Pro Ala Phe Pro Thr Gln Glu Met Lys Leu Glu Ile Ser 
130                 135                 140                 145 

GAG GCC GTG GCT CCG GGG ACG CGC TTT CCG CTC GAG AGC GCG CAC GAT      597 
Glu Ala Val Ala Pro Gly Thr Arg Phe Pro Leu Glu Ser Ala His Asp 
                150                 155                 160 

CCC GAT CTG GGA AGC AAC TCT TTA CAA ACC TAT GAG CTG AGC CGA AAT      645 
Pro Asp Leu Gly Ser Asn Ser Leu Gln Thr Tyr Glu Leu Ser Arg Asn 
            165                 170                 175 

GAA TAC TTT GCG CTT CGC GTG CAG ACG CGG GAG GAC AGC ACC AAG TAC      693 
Glu Tyr Phe Ala Leu Arg Val Gln Thr Arg Glu Asp Ser Thr Lys Tyr 
        180                 185                 190 

GCG GAG CTG GTG TTG GAG CGC GCC CTG GAC CGA GAA CGG GAG CCT AGT      741 
Ala Glu Leu Val Leu Glu Arg Ala Leu Asp Arg Glu Arg Glu Pro Ser 
    195                 200                 205 

CTC CAG TTA GTG CTG ACG GCG TTG GAC GGA GGG ACC CCA GCT CTC TCC      789 
Leu Gln Leu Val Leu Thr Ala Leu Asp Gly Gly Thr Pro Ala Leu Ser 
210                 215                 220                 225 

GCC AGC CTG CCT ATT CAC ATC AAG GTG CTG GAC GCG AAT GAC AAT GCG      837 
Ala Ser Leu Pro Ile His Ile Lys Val Leu Asp Ala Asn Asp Asn Ala 
                230                 235                 240 

CCT GTC TTC AAC CAG TCC TTG TAC CGG GCG CGC GTT CCT GGA GGA TGC      885 
Pro Val Phe Asn Gln Ser Leu Tyr Arg Ala Arg Val Pro Gly Gly Cys 
            245                 250                 255 

ACC TCC GGC ACG CGC GTG GTA CAA GTC CTT GCA ACG GAT CTG GAT GAA      933 
Thr Ser Gly Thr Arg Val Val Gln Val Leu Ala Thr Asp Leu Asp Glu 
        260                 265                 270 

GGC CCC AAC GGT GAA ATT ATT TAC TCC TTC GGC AGC CAC AAC CGC GCC      981 
Gly Pro Asn Gly Glu Ile Ile Tyr Ser Phe Gly Ser His Asn Arg Ala 
    275                 280                 285 

GGC GTG CGG CAA CTA TTC GCC TTA GAC CTT GTA ACC GGG ATG CTG ACA     1029 
Gly Val Arg Gln Leu Phe Ala Leu Asp Leu Val Thr Gly Met Leu Thr 
290                 295                 300                 305 

ATC AAG GGT CGG CTG GAC TTC GAG GAC ACC AAA CTC CAT GAG ATT TAC     1077 
Ile Lys Gly Arg Leu Asp Phe Glu Asp Thr Lys Leu His Glu Ile Tyr 
                310                 315                 320 

ATC CAG GCC AAA GAC AAG GGC GCC AAT CCC GAA GGA GCA CAT TGC AAA     1125 
Ile Gln Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys Lys 
            325                 330                 335 

GTG TTG GTG GAG GTT GTG GAT GTG AAT GAC AAC GCC CCG GAG ATC ACA     1173 
Val Leu Val Glu Val Val Asp Val Asn Asp Asn Ala Pro Glu Ile Thr 
        340                 345                 350 

GTC ACC TCC GTG TAC AGC CCA GTA CCC GAG GAT GCC TCT GGG ACT GTC     1221 
Val Thr Ser Val Tyr Ser Pro Val Pro Glu Asp Ala Ser Gly Thr Val 
    355                 360                 365 

ATC GCT TTG CTC AGT GTG ACT GAC CTG GAT GCT GGC GAG AAC GGG CTG     1269 
Ile Ala Leu Leu Ser Val Thr Asp Leu Asp Ala Gly Glu Asn Gly Leu 
370                 375                 380                 385 

GTG ACC TGC GAA GTT CCA CCG GGT CTC CCT TTC AGC CTT ACT TCT TCC     1317 
Val Thr Cys Glu Val Pro Pro Gly Leu Pro Phe Ser Leu Thr Ser Ser 
                390                 395                 400 

CTC AAG AAT TAC TTC ACT TTG AAA ACC AGT GCA GAC CTG GAT CGG GAG     1365 
Leu Lys Asn Tyr Phe Thr Leu Lys Thr Ser Ala Asp Leu Asp Arg Glu 
            405                 410                 415 

ACT GTG CCA GAA TAC AAC CTC AGC ATC ACC GCC CGA GAC GCC GGA ACC     1413 
Thr Val Pro Glu Tyr Asn Leu Ser Ile Thr Ala Arg Asp Ala Gly Thr 
        420                 425                 430 

CCT TCC CTC TCA GCC CTT ACA ATA GTG CGT GTT CAA GTG TCC GAC ATC     1461 
Pro Ser Leu Ser Ala Leu Thr Ile Val Arg Val Gln Val Ser Asp Ile 
    435                 440                 445 

AAT GAC AAC CCT CCA CAA TCT TCT CAA TCT TCC TAC GAC GTT TAC ATT     1509 
Asn Asp Asn Pro Pro Gln Ser Ser Gln Ser Ser Tyr Asp Val Tyr Ile 
450                 455                 460                 465 

GAA GAA AAC AAC CTC CCC GGG GCT CCA ATA CTA AAC CTA AGT GTC TGG     1557 
Glu Glu Asn Asn Leu Pro Gly Ala Pro Ile Leu Asn Leu Ser Val Trp 
                470                 475                 480 

GAC CCC GAC GCC CCG CAG AAT GCT CGG CTT TCT TTC TTT CTC TTG GAG     1605 
Asp Pro Asp Ala Pro Gln Asn Ala Arg Leu Ser Phe Phe Leu Leu Glu 
            485                 490                 495 

CAA GGA GCT GAA ACC GGG CTA GTG GGT CGC TAT TTC ACA ATA AAT CGT     1653 
Gln Gly Ala Glu Thr Gly Leu Val Gly Arg Tyr Phe Thr Ile Asn Arg 
        500                 505                 510 

GAC AAT GGC ATA GTG TCA TCC TTA GTG CCC CTA GAC TAT GAG GAT CGG     1701 
Asp Asn Gly Ile Val Ser Ser Leu Val Pro Leu Asp Tyr Glu Asp Arg 
    515                 520                 525 

CGG GAA TTT GAA TTA ACA GCT CAT ATC AGC GAT GGG GGC ACC CCG GTC     1749 
Arg Glu Phe Glu Leu Thr Ala His Ile Ser Asp Gly Gly Thr Pro Val 
530                 535                 540                 545 

CTA GCC ACC AAC ATC AGC GTG AAC ATA TTT GTC ACT GAT CGC AAT GAC     1797 
Leu Ala Thr Asn Ile Ser Val Asn Ile Phe Val Thr Asp Arg Asn Asp 
                550                 555                 560 

AAT GCC CCC CAG GTC CTA TAT CCT CGG CCA GGT GGG AGC TCG GTG GAG     1845 
Asn Ala Pro Gln Val Leu Tyr Pro Arg Pro Gly Gly Ser Ser Val Glu 
            565                 570                 575 

ATG CTG CCT CGA GGT ACC TCA GCT GGC CAC CTA GTG TCA CGG GTG GTA     1893 
Met Leu Pro Arg Gly Thr Ser Ala Gly His Leu Val Ser Arg Val Val 
        580                 585                 590 

GGC TGG GAC GCG GAT GCA GGG CAC AAT GCC TGG CTC TCC TAC AGT CTC     1941 
Gly Trp Asp Ala Asp Ala Gly His Asn Ala Trp Leu Ser Tyr Ser Leu 
    595                 600                 605 

TTT GGA TCC CCT AAC CAG AGC CTT TTT GCC ATA GGG CTG CAC ACT GGT     1989 
Phe Gly Ser Pro Asn Gln Ser Leu Phe Ala Ile Gly Leu His Thr Gly 
610                 615                 620                 625 

CAA ATC AGT ACT GCC CGT CCA GTC CAA GAC ACA GAT TCA CCC AGG CAG     2037 
Gln Ile Ser Thr Ala Arg Pro Val Gln Asp Thr Asp Ser Pro Arg Gln 
                630                 635                 640 

ACT CTC ACT GTC TTG ATC AAA GAC AAT GGG GAG CCT TCG CTC TCC ACC     2085 
Thr Leu Thr Val Leu Ile Lys Asp Asn Gly Glu Pro Ser Leu Ser Thr 
            645                 650                 655 

ACT GCT ACC CTC ACT GTG TCA GTA ACC GAG GAC TCT CCT GAA GCC CGA     2133 
Thr Ala Thr Leu Thr Val Ser Val Thr Glu Asp Ser Pro Glu Ala Arg 
        660                 665                 670 

GCC GAG TTC CCC TCT GGC TCT GCC CCC CGG GAG CAG AAA AAA AAT CTC     2181 
Ala Glu Phe Pro Ser Gly Ser Ala Pro Arg Glu Gln Lys Lys Asn Leu 
    675                 680                 685 

ACC TTT TAT CTA CTT CTT TCT CTA ATC CTG GTT TCT GTG GGC TTC GTG     2229 
Thr Phe Tyr Leu Leu Leu Ser Leu Ile Leu Val Ser Val Gly Phe Val 
690                 695                 700                 705 

GTC ACA GTG TTC GGA GTA ATC ATA TTC AAA GTT TAC AAG TGG AAG CAG     2277 
Val Thr Val Phe Gly Val Ile Ile Phe Lys Val Tyr Lys Trp Lys Gln 
                710                 715                 720 

TCT AGA GAC CTA TAC CGA GCC CCG GTG AGC TCA CTG TAC CGA ACA CCA     2325 
Ser Arg Asp Leu Tyr Arg Ala Pro Val Ser Ser Leu Tyr Arg Thr Pro 
            725                 730                 735 

GGG CCC TCC TTG CAC GCG GAC GCC GTG CGG GGA GGC CTG ATG TCG CCG     2373 
Gly Pro Ser Leu His Ala Asp Ala Val Arg Gly Gly Leu Met Ser Pro 
        740                 745                 750 

CAC CTT TAC CAT CAG GTG TAT CTC ACC ACG GAC TCC CGC CGC AGC GAC     2421 
His Leu Tyr His Gln Val Tyr Leu Thr Thr Asp Ser Arg Arg Ser Asp 
    755                 760                 765 

CCG CTG CTG AAG AAA CCT GGT GCA GCC AGT CCA CTG GCC AGC CGC CAG     2469 
Pro Leu Leu Lys Lys Pro Gly Ala Ala Ser Pro Leu Ala Ser Arg Gln 
770                 775                 780                 785 

AAC ACG CTG CGG AGC TGT GAT CCG GTG TTC TAT AGG CAG GTG TTG GGT     2517 
Asn Thr Leu Arg Ser Cys Asp Pro Val Phe Tyr Arg Gln Val Leu Gly 
                790                 795                 800 

GCA GAG AGC GCC CCT CCC GGA CAG CAA GCC CCG CCC AAC ACG GAC TGG     2565 
Ala Glu Ser Ala Pro Pro Gly Gln Gln Ala Pro Pro Asn Thr Asp Trp 
            805                 810                 815 

CGT TTC TCT CAG GCC CAG AGA CCC GGC ACC AGC GGC TCC CAA AAT GGC     2613 
Arg Phe Ser Gln Ala Gln Arg Pro Gly Thr Ser Gly Ser Gln Asn Gly 
        820                 825                 830 

GAT GAC ACC GGC ACC TGG CCC AAC AAC CAG TTT GAC ACA GAG ATG CTG     2661 
Asp Asp Thr Gly Thr Trp Pro Asn Asn Gln Phe Asp Thr Glu Met Leu 
    835                 840                 845 

CAA GCC ATG ATC TTG GCG TCC GCC AGT GAA GCT GCT GAT GGG AGC TCC     2709 
Gln Ala Met Ile Leu Ala Ser Ala Ser Glu Ala Ala Asp Gly Ser Ser 
850                 855                 860                 865 

ACC CTG GGA GGG GGT GCC GGC ACC ATG GGA TTG AGC GCC CGC TAC GGA     2757 
Thr Leu Gly Gly Gly Ala Gly Thr Met Gly Leu Ser Ala Arg Tyr Gly 
                870                 875                 880 

CCC CAG TTC ACC CTG CAG CAC GTG CCC GAC TAC CGC CAG AAT GTC TAC     2805 
Pro Gln Phe Thr Leu Gln His Val Pro Asp Tyr Arg Gln Asn Val Tyr 
            885                 890                 895 

ATC CCA GGC AGC AAT GCA CAC T GACCAACGCA GCTGGCAAGC GGATGGCAAG      2857 
Ile Pro Gly Ser Asn Ala His 
        900 

GCCCAGCAGG TGGCAATGGC AACAAGAAGA AGTCGGCAAG AAGGAGAAGA AGTAACATGG   2917 

AGGCCAGGCC AAGAGCCACA GGGCAGCCTC TCCCCGAACC AGCCCAGCTT CTCCTTACCT   2977 

GCACCCAGGC CTCAGAGTTT CAGGGCTAAC CCCCAGAATA CTGGTAGGGG CCAAGGCATC   3037 

TCCCTTGGAA ACAGAAACAA GTGCCATCAC ACCATCCCTT CCCCAGGTGT AATATCCAAA   3097 

GCAGTTCCGC TGGGAACCCC ATCCAATCAG TGGCTGTACC CATTTGGGTA GTGGGGTTCA   3157 

TGTAGACACC AAGAACCATT TGCCACACCC CGTTTAGTTA CAGCTGAACC CTCCATCTTC   3217 

CAAATCAATC AGGCCCATCC ATCCCATGCC TCCCTCCTCC CCACCCCACT CCAACAGTTC   3277 

CTCTTTCCCG AGTAAGGTGG TTGGGGTGTT GAAGTACCAA GTAACCTACA AGCCTCCTAG   3337 

TTCTGAAAAG TTGGAAGGGC ATCATGACCT CTTGGCCTCT CCTTTGATTC TCAATCTTCC   3397 

CCCAAAGCAT GGTTTGGTGC CAGCCCCTTC ACCTCCTTCC AGAGCCCAAG ATCAATGCTC   3457 

AAGTTTTGGA GGACATGATC ACCATCCCCA TGGTACTGAT GCTTGCTGGA TTTAGGGAGG   3517 

GCATTTTGCT ACCAAGCCTC TTCCCAACGC CCTGGGACCA GTCTTCTGTT TTGTTTTTCA   3577 

TTGTTTGAGC TTTCCACTGC ATGCCTTGAC TTCCCCCACC TCCTCCTCAA ACAAGAGACT   3637 

CCACTGCATG TTCCAAGACA GTATGGGGTG GTAAGATAAG GAAGGGAAGT GTGTGGATGT   3697 

GGATGGTGGG GGCATGGACA AAGCTTGACA CATCAAGTTA TCAAGGCCTT GGAGGAGGCT   3757 

CTGTATGTCC TCAGGGGACT GACAACATCC TCCAGATTCC AGCCATAAAC CAATAACTAG   3817 

GCTGGACCCT TCCCACTACA TAATAGGGCT CAGCCAGGCA GCCAGCTTTG GGCTGAGCTA   3877 

ACAGGACCAA TGGATTAACT GGCATTTCAG TCCAAGGAAG CTCGAAGCAG GTTTAGGACC   3937 

AGGTCCCCTT GAGAGGTCAG AGGGGCCTCT GTGGGTGCTG GGTACTCCAG AGGTGCCACT   3997 

GGTGGAAGGG TCAGCGGAGC CCCAGCAGGA AGGGTGGGCC AGCCAGGCCA TTCTTAGTCC   4057 

CTGGGTTGGG GAGGCAGGGA GCTAGGGCAG GGACCAAATG AACAGAAAGT CTCAGCCCAG   4117 

GATGGGGCTT CTTCAACAGG CCCCTGCCCT CCTGAAGCCT CAGTCCTTCA CCTTGCCAGG   4177 

TGCCGTTTCT CTTCCGTGAA GGCCACTGCC CAGGTCCCCA GTGCGCCCCC TAGTGGCCAT   4237 

AGCCTGGTTA AAGTTCCCCA GTGCCTCCTT GTGATAGACC TTCTTCTCCC ACCCCCTTCT   4297 

GCCCCTGGGT CCCCGGCCAT CCAGCGGGGC TGCCAGAGAA CCCCAGACCT GCCCTTACAG   4357 

TAGTGTAGCG CCCCCTCCCT CTTTCGGCTG GTGTAGAATA GCCAGTAGTG TAGTGCGGTG   4417 

TGCTTTTACG TGATGGCGGG TGGGCAGCGG GCGGCGGCGT CCGCGCAGCC GTCTGTCCTT   4477 

GATCTGCCCG CGGCGGCCCG TGTTGTGTTT TGTGCTGTGT CCAGCGCTAA GGCGACCCCC   4537 

TCCCCCGTAC TGACTTCTCC TATAAGCGCT TCTCTTCGCA TAGTCACGTA GCTCCCACCC   4597 

CACCCTCTTC CTGTGTCTCA CGCAAGTTTT ATACTCTAAT ATTTATATGG CTTTTTTTCT   4657 

TCGACAAAAA AATAATAAAA CGTTTCTTCT GAAAAAAAAA AAAAAAAA                4705 

 
           
           
             
               904 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              97 

Met Val Pro Glu Ala Trp Arg Ser Gly Leu Val Ser Thr Gly Arg Val 
  1               5                  10                  15 

Val Gly Val Leu Leu Leu Leu Gly Ala Leu Asn Lys Ala Ser Thr Val 
             20                  25                  30 

Ile His Tyr Glu Ile Pro Glu Glu Arg Glu Lys Gly Phe Ala Val Gly 
         35                  40                  45 

Asn Val Val Ala Asn Leu Gly Leu Asp Leu Gly Ser Leu Ser Ala Arg 
     50                  55                  60 

Arg Phe Pro Val Val Ser Gly Ala Ser Arg Arg Phe Phe Glu Val Asn 
 65                  70                  75                  80 

Arg Glu Thr Gly Glu Met Phe Val Asn Asp Arg Leu Asp Arg Glu Glu 
                 85                  90                  95 

Leu Cys Gly Thr Leu Pro Ser Cys Thr Val Thr Leu Glu Leu Val Val 
            100                 105                 110 

Glu Asn Pro Leu Glu Leu Phe Ser Val Glu Val Val Ile Gln Asp Ile 
        115                 120                 125 

Asn Asp Asn Asn Pro Ala Phe Pro Thr Gln Glu Met Lys Leu Glu Ile 
    130                 135                 140 

Ser Glu Ala Val Ala Pro Gly Thr Arg Phe Pro Leu Glu Ser Ala His 
145                 150                 155                 160 

Asp Pro Asp Leu Gly Ser Asn Ser Leu Gln Thr Tyr Glu Leu Ser Arg 
                165                 170                 175 

Asn Glu Tyr Phe Ala Leu Arg Val Gln Thr Arg Glu Asp Ser Thr Lys 
            180                 185                 190 

Tyr Ala Glu Leu Val Leu Glu Arg Ala Leu Asp Arg Glu Arg Glu Pro 
        195                 200                 205 

Ser Leu Gln Leu Val Leu Thr Ala Leu Asp Gly Gly Thr Pro Ala Leu 
    210                 215                 220 

Ser Ala Ser Leu Pro Ile His Ile Lys Val Leu Asp Ala Asn Asp Asn 
225                 230                 235                 240 

Ala Pro Val Phe Asn Gln Ser Leu Tyr Arg Ala Arg Val Pro Gly Gly 
                245                 250                 255 

Cys Thr Ser Gly Thr Arg Val Val Gln Val Leu Ala Thr Asp Leu Asp 
            260                 265                 270 

Glu Gly Pro Asn Gly Glu Ile Ile Tyr Ser Phe Gly Ser His Asn Arg 
        275                 280                 285 

Ala Gly Val Arg Gln Leu Phe Ala Leu Asp Leu Val Thr Gly Met Leu 
    290                 295                 300 

Thr Ile Lys Gly Arg Leu Asp Phe Glu Asp Thr Lys Leu His Glu Ile 
305                 310                 315                 320 

Tyr Ile Gln Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys 
                325                 330                 335 

Lys Val Leu Val Glu Val Val Asp Val Asn Asp Asn Ala Pro Glu Ile 
            340                 345                 350 

Thr Val Thr Ser Val Tyr Ser Pro Val Pro Glu Asp Ala Ser Gly Thr 
        355                 360                 365 

Val Ile Ala Leu Leu Ser Val Thr Asp Leu Asp Ala Gly Glu Asn Gly 
    370                 375                 380 

Leu Val Thr Cys Glu Val Pro Pro Gly Leu Pro Phe Ser Leu Thr Ser 
385                 390                 395                 400 

Ser Leu Lys Asn Tyr Phe Thr Leu Lys Thr Ser Ala Asp Leu Asp Arg 
                405                 410                 415 

Glu Thr Val Pro Glu Tyr Asn Leu Ser Ile Thr Ala Arg Asp Ala Gly 
            420                 425                 430 

Thr Pro Ser Leu Ser Ala Leu Thr Ile Val Arg Val Gln Val Ser Asp 
        435                 440                 445 

Ile Asn Asp Asn Pro Pro Gln Ser Ser Gln Ser Ser Tyr Asp Val Tyr 
    450                 455                 460 

Ile Glu Glu Asn Asn Leu Pro Gly Ala Pro Ile Leu Asn Leu Ser Val 
465                 470                 475                 480 

Trp Asp Pro Asp Ala Pro Gln Asn Ala Arg Leu Ser Phe Phe Leu Leu 
                485                 490                 495 

Glu Gln Gly Ala Glu Thr Gly Leu Val Gly Arg Tyr Phe Thr Ile Asn 
            500                 505                 510 

Arg Asp Asn Gly Ile Val Ser Ser Leu Val Pro Leu Asp Tyr Glu Asp 
        515                 520                 525 

Arg Arg Glu Phe Glu Leu Thr Ala His Ile Ser Asp Gly Gly Thr Pro 
    530                 535                 540 

Val Leu Ala Thr Asn Ile Ser Val Asn Ile Phe Val Thr Asp Arg Asn 
545                 550                 555                 560 

Asp Asn Ala Pro Gln Val Leu Tyr Pro Arg Pro Gly Gly Ser Ser Val 
                565                 570                 575 

Glu Met Leu Pro Arg Gly Thr Ser Ala Gly His Leu Val Ser Arg Val 
            580                 585                 590 

Val Gly Trp Asp Ala Asp Ala Gly His Asn Ala Trp Leu Ser Tyr Ser 
        595                 600                 605 

Leu Phe Gly Ser Pro Asn Gln Ser Leu Phe Ala Ile Gly Leu His Thr 
    610                 615                 620 

Gly Gln Ile Ser Thr Ala Arg Pro Val Gln Asp Thr Asp Ser Pro Arg 
625                 630                 635                 640 

Gln Thr Leu Thr Val Leu Ile Lys Asp Asn Gly Glu Pro Ser Leu Ser 
                645                 650                 655 

Thr Thr Ala Thr Leu Thr Val Ser Val Thr Glu Asp Ser Pro Glu Ala 
            660                 665                 670 

Arg Ala Glu Phe Pro Ser Gly Ser Ala Pro Arg Glu Gln Lys Lys Asn 
        675                 680                 685 

Leu Thr Phe Tyr Leu Leu Leu Ser Leu Ile Leu Val Ser Val Gly Phe 
    690                 695                 700 

Val Val Thr Val Phe Gly Val Ile Ile Phe Lys Val Tyr Lys Trp Lys 
705                 710                 715                 720 

Gln Ser Arg Asp Leu Tyr Arg Ala Pro Val Ser Ser Leu Tyr Arg Thr 
                725                 730                 735 

Pro Gly Pro Ser Leu His Ala Asp Ala Val Arg Gly Gly Leu Met Ser 
            740                 745                 750 

Pro His Leu Tyr His Gln Val Tyr Leu Thr Thr Asp Ser Arg Arg Ser 
        755                 760                 765 

Asp Pro Leu Leu Lys Lys Pro Gly Ala Ala Ser Pro Leu Ala Ser Arg 
    770                 775                 780 

Gln Asn Thr Leu Arg Ser Cys Asp Pro Val Phe Tyr Arg Gln Val Leu 
785                 790                 795                 800 

Gly Ala Glu Ser Ala Pro Pro Gly Gln Gln Ala Pro Pro Asn Thr Asp 
                805                 810                 815 

Trp Arg Phe Ser Gln Ala Gln Arg Pro Gly Thr Ser Gly Ser Gln Asn 
            820                 825                 830 

Gly Asp Asp Thr Gly Thr Trp Pro Asn Asn Gln Phe Asp Thr Glu Met 
        835                 840                 845 

Leu Gln Ala Met Ile Leu Ala Ser Ala Ser Glu Ala Ala Asp Gly Ser 
    850                 855                 860 

Ser Thr Leu Gly Gly Gly Ala Gly Thr Met Gly Leu Ser Ala Arg Tyr 
865                 870                 875                 880 

Gly Pro Gln Phe Thr Leu Gln His Val Pro Asp Tyr Arg Gln Asn Val 
                885                 890                 895 

Tyr Ile Pro Gly Ser Asn Ala His 
            900 

 
           
           
             
               556 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              98 

Asp Trp Val Ile Pro Pro Ile Asn Leu Pro Glu Asn Ser Arg Gly Pro 
1               5                   10                  15 

Phe Pro Gln Glu Leu Val Arg Ile Arg Ser Asp Arg Asp Lys Asn Leu 
            20                  25                  30 

Ser Leu Arg Tyr Thr Val Thr Gly Pro Gly Ala Asp Gln Pro Pro Thr 
        35                  40                  45 

Gly Ile Phe Ile Ile Asn Pro Ile Ser Gly Gln Leu Ser Val Thr Lys 
    50                  55                  60 

Pro Leu Asp Arg Glu Gln Ile Ala Arg Phe His Leu Arg Ala His Ala 
65                  70                  75                  80 

Val Asp Ile Asn Gly Asn Gln Val Glu Asn Pro Ile Asp Ile Val Ile 
                85                  90                  95 

Asn Val Ile Asp Met Asn Asp Asn Arg Pro Glu Phe Leu His Gln Val 
            100                 105                 110 

Trp Asn Gly Ser Val Pro Glu Gly Ser Lys Pro Gly Thr Tyr Val Met 
        115                 120                 125 

Thr Val Thr Ala Ile Asp Ala Asp Asp Pro Asn Ala Leu Asn Gly Met 
    130                 135                 140 

Leu Arg Tyr Arg Ile Leu Ser Gln Ala Pro Ser Thr Pro Ser Pro Asp 
145                 150                 155                 160 

Met Phe Thr Ile Asn Asn Glu Thr Gly Asp Ile Ile Thr Val Ala Ala 
                165                 170                 175 

Gly Leu Asp Arg Glu Lys Val Gln Gln Tyr Thr Leu Ile Ile Gln Ala 
            180                 185                 190 

Thr Asp Met Glu Gly Asn Pro Thr Tyr Gly Leu Ser Asn Thr Ala Thr 
        195                 200                 205 

Ala Val Ile Thr Val Thr Asp Val Asn Asp Asn Pro Pro Glu Phe Thr 
    210                 215                 220 

Ala Met Thr Phe Tyr Gly Glu Val Pro Glu Asn Arg Val Asp Ile Ile 
225                 230                 235                 240 

Val Ala Asn Leu Thr Val Thr Asp Lys Asp Gln Pro His Thr Pro Ala 
                245                 250                 255 

Trp Asn Ala Val Thr Arg Ile Ser Gly Gly Asp Pro Thr Gly Arg Phe 
            260                 265                 270 

Ala Ile Gln Thr Asp Pro Asn Ser Asn Asp Gly Leu Val Thr Val Val 
        275                 280                 285 

Lys Pro Ile Asp Phe Glu Thr Asn Arg Met Phe Val Leu Thr Val Ala 
    290                 295                 300 

Ala Glu Asn Gln Val Pro Leu Ala Lys Gly Ile Gln His Pro Pro Gln 
305                 310                 315                 320 

Ser Thr Ala Thr Val Ser Val Thr Val Ile Asp Val Asn Glu Asn Pro 
                325                 330                 335 

Tyr Phe Ala Pro Asn Pro Lys Ile Ile Arg Gln Glu Glu Gly Leu His 
            340                 345                 350 

Ala Gly Thr Met Leu Thr Thr Phe Thr Ala Gly Asp Pro Asp Arg Tyr 
        355                 360                 365 

Met Gln Gln Asn Ile Arg Tyr Thr Lys Leu Ser Asp Pro Ala Asn Trp 
    370                 375                 380 

Leu Lys Ile Asp Pro Val Asn Gly Gln Ile Thr Thr Ile Ala Val Leu 
385                 390                 395                 400 

Asp Arg Glu Ser Pro Asn Val Lys Asn Asn Ile Tyr Asn Ala Thr Phe 
                405                 410                 415 

Leu Ala Ser Asp Asn Gly Ile Pro Pro Met Ser Gly Thr Gly Thr Leu 
            420                 425                 430 

Gln Ile Tyr Leu Leu Asp Ile Asn Asp Asn Ala Pro Gln Val Leu Pro 
        435                 440                 445 

Gln Glu Ala Glu Thr Cys Glu Thr Pro Asp Pro Asn Ser Ile Asn Ile 
    450                 455                 460 

Thr Thr Ala Leu Asp Tyr Asp Ile Asp Pro Asn Ala Gly Pro Phe Ala 
465                 470                 475                 480 

Tyr Asp Leu Pro Leu Ser Pro Val Thr Ile Lys Arg Asn Trp Thr Ile 
                485                 490                 495 

Thr Arg Leu Asn Gly Asp Phe Ala Gln Leu Asn Leu Lys Ile Lys Phe 
            500                 505                 510 

Leu Glu Ala Gly Ile Tyr Glu Val Pro Ile Ile Ile Thr Asp Ser Gly 
        515                 520                 525 

Asn Pro Pro Lys Ser Asn Lys Ser Ile Leu Arg Val Arg Val Cys Glu 
    530                 535                 540 

Cys Asp Phe Asn Gly Asp Cys Thr Asp Val Asp Arg 
545                 550                 555 

 
           
           
             
               105 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              99 

Glu Asp Thr Val Tyr Ser Phe Asp Ile Pro Glu Asn Ala Gln Arg Gly 
1               5                   10                  15 

Tyr Gln Val Gly Gln Ile Val Ala Arg Asp Ala Asp Leu Gly Gln Asp 
            20                  25                  30 

Ala Gln Leu Ser Tyr Gly Val Val Ser Asp Trp Ala Asn Asp Val Phe 
        35                  40                  45 

Ser Leu Asn Pro Gln Thr Gly Met Leu Thr Leu Thr Ala Arg Leu Asp 
    50                  55                  60 

Tyr Glu Glu Val Gln His Tyr Ile Leu Ile Val Gln Ala Gln Asp Asp 
65                  70                  75                  80 

Gly Gln Pro Ser Leu Ser Thr Thr Ile Thr Val Tyr Cys Asn Val Leu 
                85                  90                  95 

Asp Leu Asn Asp Asn Ala Pro Ile Phe 
            100                 105 

 
           
           
             
               7 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              100 

Asp Xaa Asp Xaa Gly Xaa Asn 
1               5 

 
           
           
             
               7 amino acids  
               amino acid  
               single  
               linear  
             
             
               protein  
             
              101 

Ala Xaa Asp Xaa Gly Xaa Pro 
1               5 

 
           
           
             
               4650 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
             
               CDS  
                495..4103 

 
             
              102 

CCTCTATTCG ACATTCTCTT TGGATTGTTT TGCTATAACT TGAAATTTGG GATGTCACAA     60 

ACGAAACTGT CATCTGTTTC CGCCAAACTG TGGTTCTGCT AATCTCCCAG GCTGGCAGCA    120 

TTGGAGACTT GCTGACTTCT TTCATCCCCC ACTCTTTTCA CCTGAAATTC CTTTCCTTGG    180 

TTTTGCTCTA AGTCCTATGC TTCAGTCAGG GGCCAACCAA ATCTCACTGC CTCCTTTTTA    240 

TCATGAAGCC TTTGATCACT GATAGTTCTT TTTATATCTT GAAAAATCAC CCTTCCCAGT    300 

ACAGTTAATA TTTAGTATCT CTACTCATCT TGGCACTTAC TCACAGCTCC ATAATTCAGT    360 

CGTTTTCGTA CCTCTTCATG GTGATGGGGA GCCCTTTGGA GGTGGTGACT GTGCTTTATA    420 

CTCCTCATGA TGCTTCACAT GTGGCAGGCG TGGAGTGCCC GGAGGCGGCC CTCCTGATTC    480 

TGGGGCCTCC CAGG ATG GAG CCC CTG AGG CAC AGC CCA GGC CCT GGG GGG      530 
                Met Glu Pro Leu Arg His Ser Pro Gly Pro Gly Gly 
                  1               5                  10 

CAA CGG CTA CTG CTG CCC TCC ATG CTG CTA GCA CTG CTG CTC CTG CTG      578 
Gln Arg Leu Leu Leu Pro Ser Met Leu Leu Ala Leu Leu Leu Leu Leu 
         15                  20                  25 

GCT CCA TCC CCA GGC CAC GCC ACT CGG GTA GTG TAC AAG GTG CCG GAG      626 
Ala Pro Ser Pro Gly His Ala Thr Arg Val Val Tyr Lys Val Pro Glu 
     30                  35                  40 

GAA CAG CCA CCC AAC ACC CTC ATT GGG AGC CTC GCA GCC GAC TAT GGT      674 
Glu Gln Pro Pro Asn Thr Leu Ile Gly Ser Leu Ala Ala Asp Tyr Gly 
 45                  50                  55                  60 

TTT CCA GAT GTG GGG CAC CTG TAC AAG CTA GAG GTG GGT GCC CCG TAC      722 
Phe Pro Asp Val Gly His Leu Tyr Lys Leu Glu Val Gly Ala Pro Tyr 
                 65                  70                  75 

CTT CGC GTG GAT GGC AAG ACA GGT GAC ATT TTC ACC ACC GAG ACC TCC      770 
Leu Arg Val Asp Gly Lys Thr Gly Asp Ile Phe Thr Thr Glu Thr Ser 
             80                  85                  90 

ATC GAC CGT GAG GGG CTC CGT GAA TGC CAG AAC CAG CTC CCT GGT GAT      818 
Ile Asp Arg Glu Gly Leu Arg Glu Cys Gln Asn Gln Leu Pro Gly Asp 
         95                 100                 105 

CCC TGC ATC CTG GAG TTT GAG GTA TCT ATC ACA GAC CTC GTG CAG AAT      866 
Pro Cys Ile Leu Glu Phe Glu Val Ser Ile Thr Asp Leu Val Gln Asn 
    110                 115                 120 

GCG AGC CCC CGG CTG CTA GAG GGC CAG ATA GAA GTA CAA GAC ATC AAT      914 
Ala Ser Pro Arg Leu Leu Glu Gly Gln Ile Glu Val Gln Asp Ile Asn 
125                 130                 135                 140 

GAC AAC ACA CCC AAC TTC GCC TCA CCA GTC ATC ACT CTG GCC ATC CCT      962 
Asp Asn Thr Pro Asn Phe Ala Ser Pro Val Ile Thr Leu Ala Ile Pro 
                145                 150                 155 

GAG AAC ACC AAC ATC GGC TCA CTC TTC CCC ATC CCG CTG GCT TCA GAC     1010 
Glu Asn Thr Asn Ile Gly Ser Leu Phe Pro Ile Pro Leu Ala Ser Asp 
            160                 165                 170 

CGT GAT GCT GGT CCC AAC GGT GTG GCA TCC TAT GAG CTG CAG GTG GCA     1058 
Arg Asp Ala Gly Pro Asn Gly Val Ala Ser Tyr Glu Leu Gln Val Ala 
        175                 180                 185 

GAG GAC CAG GAG GAG AAG CAA CCA CAG CTC ATT GTG ATG GGC AAC CTG     1106 
Glu Asp Gln Glu Glu Lys Gln Pro Gln Leu Ile Val Met Gly Asn Leu 
    190                 195                 200 

GAC CGT GAG CGC TGG GAC TCC TAT GAC CTC ACC ATC AAG GTG CAG GAT     1154 
Asp Arg Glu Arg Trp Asp Ser Tyr Asp Leu Thr Ile Lys Val Gln Asp 
205                 210                 215                 220 

GGC GGC AGC CCC CCA CGC GCC ACG AGT GCC CTG CTG CGT GTC ACC GTG     1202 
Gly Gly Ser Pro Pro Arg Ala Thr Ser Ala Leu Leu Arg Val Thr Val 
                225                 230                 235 

CTT GAC ACC AAT GAC AAC GCC CCC AAG TTT GAG CGG CCC TCC TAT GAG     1250 
Leu Asp Thr Asn Asp Asn Ala Pro Lys Phe Glu Arg Pro Ser Tyr Glu 
            240                 245                 250 

GCC GAA CTA TCT GAG AAT AGC CCC ATA GGC CAC TCG GTC ATC CAG GTG     1298 
Ala Glu Leu Ser Glu Asn Ser Pro Ile Gly His Ser Val Ile Gln Val 
        255                 260                 265 

AAG GCC AAT GAC TCA GAC CAA GGT GCC AAT GCA GAA ATC GAA TAC ACA     1346 
Lys Ala Asn Asp Ser Asp Gln Gly Ala Asn Ala Glu Ile Glu Tyr Thr 
    270                 275                 280 

TTC CAC CAG GCG CCC GAA GTT GTG AGG CGT CTT CTT CGA CTG GAC AGG     1394 
Phe His Gln Ala Pro Glu Val Val Arg Arg Leu Leu Arg Leu Asp Arg 
285                 290                 295                 300 

AAC ACT GGA CTT ATC ACT GTT CAG GGC CCG GTG GAC CGT GAG GAC CTA     1442 
Asn Thr Gly Leu Ile Thr Val Gln Gly Pro Val Asp Arg Glu Asp Leu 
                305                 310                 315 

AGC ACC CTG CGC TTC TCA GTG CTT GCT AAG GAC CGA GGC ACC AAC CCC     1490 
Ser Thr Leu Arg Phe Ser Val Leu Ala Lys Asp Arg Gly Thr Asn Pro 
            320                 325                 330 

AAG AGT GCC CGT GCC CAG GTG GTT GTG ACC GTG AAG GAC ATG AAT GAC     1538 
Lys Ser Ala Arg Ala Gln Val Val Val Thr Val Lys Asp Met Asn Asp 
        335                 340                 345 

AAT GCC CCC ACC ATT GAG ATC CGG GGC ATA GGG CTA GTG ACT CAT CAA     1586 
Asn Ala Pro Thr Ile Glu Ile Arg Gly Ile Gly Leu Val Thr His Gln 
    350                 355                 360 

GAT GGG ATG GCT AAC ATC TCA GAG GAT GTG GCA GAG GAG ACA GCT GTG     1634 
Asp Gly Met Ala Asn Ile Ser Glu Asp Val Ala Glu Glu Thr Ala Val 
365                 370                 375                 380 

GCC CTG GTG CAG GTG TCT GAC CGA GAT GAG GGA GAG AAT GCA GCT GTC     1682 
Ala Leu Val Gln Val Ser Asp Arg Asp Glu Gly Glu Asn Ala Ala Val 
                385                 390                 395 

ACC TGT GTG GTG GCA GGT GAT GTG CCC TTC CAG CTG CGC CAG GCC AGT     1730 
Thr Cys Val Val Ala Gly Asp Val Pro Phe Gln Leu Arg Gln Ala Ser 
            400                 405                 410 

GAG ACA GGC AGT GAC AGC AAG AAG AAG TAT TTC CTG CAG ACT ACC ACC     1778 
Glu Thr Gly Ser Asp Ser Lys Lys Lys Tyr Phe Leu Gln Thr Thr Thr 
        415                 420                 425 

CCG CTA GAC TAC GAG AAG GTC AAA GAC TAC ACC ATT GAG ATT GTG GCT     1826 
Pro Leu Asp Tyr Glu Lys Val Lys Asp Tyr Thr Ile Glu Ile Val Ala 
    430                 435                 440 

GTG GAC TCT GGC AAC CCC CCA CTC TCC AGC ACT AAC TCC CTC AAG GTG     1874 
Val Asp Ser Gly Asn Pro Pro Leu Ser Ser Thr Asn Ser Leu Lys Val 
445                 450                 455                 460 

CAG GTG GTG GAC GTC AAT GAC AAC GCA CCT GTC TTC ACT CAG AGT GTC     1922 
Gln Val Val Asp Val Asn Asp Asn Ala Pro Val Phe Thr Gln Ser Val 
                465                 470                 475 

ACT GAG GTC GCC TTC CCG GAA AAC AAC AAG CCT GGT GAA GTG ATT GCT     1970 
Thr Glu Val Ala Phe Pro Glu Asn Asn Lys Pro Gly Glu Val Ile Ala 
            480                 485                 490 

GAG ATC ACT GCC AGT GAT GCT GAC TCT GGC TCT AAT GCT GAG CTG GTT     2018 
Glu Ile Thr Ala Ser Asp Ala Asp Ser Gly Ser Asn Ala Glu Leu Val 
        495                 500                 505 

TAC TCT CTG GAG CCT GAG CCG GCT GCT AAG GGC CTC TTC ACC ATC TCA     2066 
Tyr Ser Leu Glu Pro Glu Pro Ala Ala Lys Gly Leu Phe Thr Ile Ser 
    510                 515                 520 

CCC GAG ACT GGA GAG ATC CAG GTG AAG ACA TCT CTG GAT CGG GAA CAG     2114 
Pro Glu Thr Gly Glu Ile Gln Val Lys Thr Ser Leu Asp Arg Glu Gln 
525                 530                 535                 540 

CGG GAG AGC TAT GAG TTG AAG GTG GTG GCA GCT GAC CGG GGC AGT CCT     2162 
Arg Glu Ser Tyr Glu Leu Lys Val Val Ala Ala Asp Arg Gly Ser Pro 
                545                 550                 555 

AGC CTC CAG GGC ACA GCC ACT GTC CTT GTC AAT GTG CTG GAC TGC AAT     2210 
Ser Leu Gln Gly Thr Ala Thr Val Leu Val Asn Val Leu Asp Cys Asn 
            560                 565                 570 

GAC AAT GAC CCC AAA TTT ATG CTG AGT GGC TAC AAC TTC TCA GTG ATG     2258 
Asp Asn Asp Pro Lys Phe Met Leu Ser Gly Tyr Asn Phe Ser Val Met 
        575                 580                 585 

GAG AAC ATG CCA GCA CTG AGT CCA GTG GGC ATG GTG ACT GTC ATT GAT     2306 
Glu Asn Met Pro Ala Leu Ser Pro Val Gly Met Val Thr Val Ile Asp 
    590                 595                 600 

GGA GAC AAG GGG GAG AAT GCC CAG GTG CAG CTC TCA GTG GAG CAG GAC     2354 
Gly Asp Lys Gly Glu Asn Ala Gln Val Gln Leu Ser Val Glu Gln Asp 
605                 610                 615                 620 

AAC GGT GAC TTT GTT ATC CAG AAT GGC ACA GGC ACC ATC CTA TCC AGC     2402 
Asn Gly Asp Phe Val Ile Gln Asn Gly Thr Gly Thr Ile Leu Ser Ser 
                625                 630                 635 

CTG AGC TTT GAT CGA GAG CAA CAA AGC ACC TAC ACC TTC CAG CTG AAG     2450 
Leu Ser Phe Asp Arg Glu Gln Gln Ser Thr Tyr Thr Phe Gln Leu Lys 
            640                 645                 650 

GCA GTG GAT GGT GGC GTC CCA CCT CGC TCA GCT TAC GTT GGT GTC ACC     2498 
Ala Val Asp Gly Gly Val Pro Pro Arg Ser Ala Tyr Val Gly Val Thr 
        655                 660                 665 

ATC AAT GTG CTG GAC GAG AAT GAC AAC GCA CCC TAT ATC ACT GCC CCT     2546 
Ile Asn Val Leu Asp Glu Asn Asp Asn Ala Pro Tyr Ile Thr Ala Pro 
    670                 675                 680 

TCT AAC ACC TCT CAC AAG CTG CTG ACC CCC CAG ACA CGT CTT GGT GAG     2594 
Ser Asn Thr Ser His Lys Leu Leu Thr Pro Gln Thr Arg Leu Gly Glu 
685                 690                 695                 700 

ACG GTC AGC CAG GTG GCA GCC GAG GAC TTT GAC TCT GGT GTC AAT GCC     2642 
Thr Val Ser Gln Val Ala Ala Glu Asp Phe Asp Ser Gly Val Asn Ala 
                705                 710                 715 

GAG CTG ATC TAC AGC ATT GCA GGT GGC AAC CCT TAT GGA CTC TTC CAG     2690 
Glu Leu Ile Tyr Ser Ile Ala Gly Gly Asn Pro Tyr Gly Leu Phe Gln 
            720                 725                 730 

ATT GGG TCA CAT TCA GGT GCC ATC ACC CTG GAG AAG GAG ATT GAG CGG     2738 
Ile Gly Ser His Ser Gly Ala Ile Thr Leu Glu Lys Glu Ile Glu Arg 
        735                 740                 745 

CGC CAC CAT GGG CTA CAC CGC CTG GTG GTG AAG GTC AGT GAC CGC GGC     2786 
Arg His His Gly Leu His Arg Leu Val Val Lys Val Ser Asp Arg Gly 
    750                 755                 760 

AAG CCC CCA CGC TAT GGC ACA GCC TTG GTC CAT CTT TAT GTC AAT GAG     2834 
Lys Pro Pro Arg Tyr Gly Thr Ala Leu Val His Leu Tyr Val Asn Glu 
765                 770                 775                 780 

ACT CTG GCC AAC CGC ACG CTG CTG GAG ACC CTC CTG GGC CAC AGC CTG     2882 
Thr Leu Ala Asn Arg Thr Leu Leu Glu Thr Leu Leu Gly His Ser Leu 
                785                 790                 795 

GAC ACG CCG CTG GAT ATT GAC ATT GCT GGG GAT CCA GAA TAT GAG CGC     2930 
Asp Thr Pro Leu Asp Ile Asp Ile Ala Gly Asp Pro Glu Tyr Glu Arg 
            800                 805                 810 

TCC AAG CAG CGT GGC AAC ATT CTC TTT GGT GTG GTG GCT GGT GTG GTG     2978 
Ser Lys Gln Arg Gly Asn Ile Leu Phe Gly Val Val Ala Gly Val Val 
        815                 820                 825 

GCC GTG GCC TTG CTC ATC GCC CTG GCG GTT CTT GTG CGC TAC TGC AGA     3026 
Ala Val Ala Leu Leu Ile Ala Leu Ala Val Leu Val Arg Tyr Cys Arg 
    830                 835                 840 

CAG CGG GAG GCC AAA AGT GGT TAC CAG GCT GGT AAG AAG GAG ACC AAG     3074 
Gln Arg Glu Ala Lys Ser Gly Tyr Gln Ala Gly Lys Lys Glu Thr Lys 
845                 850                 855                 860 

GAC CTG TAT GCC CCC AAG CCC AGT GGC AAG GCC TCC AAG GGA AAC AAA     3122 
Asp Leu Tyr Ala Pro Lys Pro Ser Gly Lys Ala Ser Lys Gly Asn Lys 
                865                 870                 875 

AGC AAA GGC AAG AAG AGC AAG TCC CCA AAG CCC GTG AAG CCA GTG GAG     3170 
Ser Lys Gly Lys Lys Ser Lys Ser Pro Lys Pro Val Lys Pro Val Glu 
            880                 885                 890 

GAC GAG GAT GAG GCC GGG CTG CAG AAG TCC CTC AAG TTC AAC CTG ATG     3218 
Asp Glu Asp Glu Ala Gly Leu Gln Lys Ser Leu Lys Phe Asn Leu Met 
        895                 900                 905 

AGC GAT GCC CCT GGG GAC AGT CCC CGC ATC CAC CTG CCC CTC AAC TAC     3266 
Ser Asp Ala Pro Gly Asp Ser Pro Arg Ile His Leu Pro Leu Asn Tyr 
    910                 915                 920 

CCA CCA GGC AGC CCT GAC CTG GGC CGC CAC TAT CGC TCT AAC TCC CCA     3314 
Pro Pro Gly Ser Pro Asp Leu Gly Arg His Tyr Arg Ser Asn Ser Pro 
925                 930                 935                 940 

CTG CCT TCC ATC CAG CTG CAG CCC CAG TCA CCC TCA GCC TCC AAG AAG     3362 
Leu Pro Ser Ile Gln Leu Gln Pro Gln Ser Pro Ser Ala Ser Lys Lys 
                945                 950                 955 

CAC CAG GTG GTA CAG GAC CTG CCA CCT GCA AAC ACA TTC GTG GGC ACC     3410 
His Gln Val Val Gln Asp Leu Pro Pro Ala Asn Thr Phe Val Gly Thr 
            960                 965                 970 

GGG GAC ACC ACG TCC ACG GGC TCT GAG CAG TAC TCC GAC TAC AGC TAC     3458 
Gly Asp Thr Thr Ser Thr Gly Ser Glu Gln Tyr Ser Asp Tyr Ser Tyr 
        975                 980                 985 

CGC ACC AAC CCC CCC AAA TAC CCC AGC AAG CAG TTA CCT CAC CGC CGC     3506 
Arg Thr Asn Pro Pro Lys Tyr Pro Ser Lys Gln Leu Pro His Arg Arg 
    990                 995                 1000 

GTC ACC TTC TCG GCC ACC AGC CAG GCC CAG GAG CTG CAG GAC CCA TCC     3554 
Val Thr Phe Ser Ala Thr Ser Gln Ala Gln Glu Leu Gln Asp Pro Ser 
1005                1010                1015                1020 

CAG CAC AGT TAC TAT GAC AGT GGC CTG GAG GAG TCT GAG ACG CCG TCC     3602 
Gln His Ser Tyr Tyr Asp Ser Gly Leu Glu Glu Ser Glu Thr Pro Ser 
                1025                1030                1035 

AGC AAG TCA TCC TCA GGG CCT CGA CTC GGT CCC CTG GCC CTG CCT GAG     3650 
Ser Lys Ser Ser Ser Gly Pro Arg Leu Gly Pro Leu Ala Leu Pro Glu 
            1040                1045                1050 

GAT CAC TAT GAG CGC ACC ACC CCT GAT GGC AGC ATA GGA GAG ATG GAG     3698 
Asp His Tyr Glu Arg Thr Thr Pro Asp Gly Ser Ile Gly Glu Met Glu 
        1055                1060                1065 

CAC CCC GAG AAT GAC CTT CGC CCT TTG CCT GAT GTC GCC ATG ACA GGC     3746 
His Pro Glu Asn Asp Leu Arg Pro Leu Pro Asp Val Ala Met Thr Gly 
    1070                1075                1080 

ACA TGT ACC CGG GAG TGC AGT GAG TTT GGC CAC TCT GAC ACA TGC TGG     3794 
Thr Cys Thr Arg Glu Cys Ser Glu Phe Gly His Ser Asp Thr Cys Trp 
1085                1090                1095                1100 

ATG CCT GGC CAG TCA TCT CCC AGC CGC CGG ACC AAG AGC AGC GCC CTC     3842 
Met Pro Gly Gln Ser Ser Pro Ser Arg Arg Thr Lys Ser Ser Ala Leu 
                1105                1110                1115 

AAA CTC TCC ACC TTC ATG CCT TAC CAG GAC CGA GGA GGG CAG GAG CCT     3890 
Lys Leu Ser Thr Phe Met Pro Tyr Gln Asp Arg Gly Gly Gln Glu Pro 
            1120                1125                1130 

GCG GGC GCC GGC AGC CCC AGC CCC CCG GAA GAC CGG AAC ACC AAA ACG     3938 
Ala Gly Ala Gly Ser Pro Ser Pro Pro Glu Asp Arg Asn Thr Lys Thr 
        1135                1140                1145 

GCC CCC GTG CGC CTC CTG CCC TCC TAC AGT GCC TTC TCC CAC AGT AGC     3986 
Ala Pro Val Arg Leu Leu Pro Ser Tyr Ser Ala Phe Ser His Ser Ser 
    1150                1155                1160 

CAT GAT TCC TGC AAG GAC TCG GCC ACC TTG GAG GAA ATC CCC CTG ACC     4034 
His Asp Ser Cys Lys Asp Ser Ala Thr Leu Glu Glu Ile Pro Leu Thr 
1165                1170                1175                1180 

CAG ACC TCG GAC TTC CCA CCC GCA GCC ACA CCG GCA TCT GCC CAG ACG     4082 
Gln Thr Ser Asp Phe Pro Pro Ala Ala Thr Pro Ala Ser Ala Gln Thr 
                1185                1190                1195 

GCC AAG CGC GAG ATC TAC CTG TGAGCCCCCT ACTGGCCGGC CCCCCTCCCC        4133 
Ala Lys Arg Glu Ile Tyr Leu 
            1200 

CAGCGCCGGC CAGCTCCCAA ATGCCCATTC CAGGGCCTCA CTCTCCACCC CTTCAGCGTG   4193 

GACTTCCTGC CAGGGCCCAA GTGGGGGTAT CACTGACCTC ATGACCACGC TGGCCCTTCT   4253 

CCCATGCAGG GTCCAGGTCC TCTCCCCTCA TTTCCATCTC CCAGCCCAGG GGCCCCTTCC   4313 

CCTTTATGGG GCTTCCCCCA GCTGATGCCC AAGAGGGCTC CTCTGCAATG ACTGGGCTCC   4373 

TTCCCTTGAC TTCCAGGGAG CACCCCCTCG ATTTGGGCAG ATGGTGGAGT CAAGGGTGGG   4433 

CAGCGTACTT CTAACTCATT GTTTCCCTCA TGGCCGACCA GGGCGGGGAT AGCATGCCCA   4493 

ATTTTAGCCC TGAAGCAGGG CTGAACTGGG GAGCCCCTTT CCCTGGGAGC TCCCAGAGGA   4553 

AACTCTTGAC CACCAGTGGC TCCCTGAAGG GCTTTTGTTA CCAAAGGTGG GGTAGGGACG   4613 

GGGGTGGGAG TGGAGCGGAG GCCTTGTTTT CCCGTGG                            4650 

 
           
           
             
               1203 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              103 

Met Glu Pro Leu Arg His Ser Pro Gly Pro Gly Gly Gln Arg Leu Leu 
  1               5                  10                  15 

Leu Pro Ser Met Leu Leu Ala Leu Leu Leu Leu Leu Ala Pro Ser Pro 
             20                  25                  30 

Gly His Ala Thr Arg Val Val Tyr Lys Val Pro Glu Glu Gln Pro Pro 
         35                  40                  45 

Asn Thr Leu Ile Gly Ser Leu Ala Ala Asp Tyr Gly Phe Pro Asp Val 
     50                  55                  60 

Gly His Leu Tyr Lys Leu Glu Val Gly Ala Pro Tyr Leu Arg Val Asp 
 65                  70                  75                  80 

Gly Lys Thr Gly Asp Ile Phe Thr Thr Glu Thr Ser Ile Asp Arg Glu 
                 85                  90                  95 

Gly Leu Arg Glu Cys Gln Asn Gln Leu Pro Gly Asp Pro Cys Ile Leu 
            100                 105                 110 

Glu Phe Glu Val Ser Ile Thr Asp Leu Val Gln Asn Ala Ser Pro Arg 
        115                 120                 125 

Leu Leu Glu Gly Gln Ile Glu Val Gln Asp Ile Asn Asp Asn Thr Pro 
    130                 135                 140 

Asn Phe Ala Ser Pro Val Ile Thr Leu Ala Ile Pro Glu Asn Thr Asn 
145                 150                 155                 160 

Ile Gly Ser Leu Phe Pro Ile Pro Leu Ala Ser Asp Arg Asp Ala Gly 
                165                 170                 175 

Pro Asn Gly Val Ala Ser Tyr Glu Leu Gln Val Ala Glu Asp Gln Glu 
            180                 185                 190 

Glu Lys Gln Pro Gln Leu Ile Val Met Gly Asn Leu Asp Arg Glu Arg 
        195                 200                 205 

Trp Asp Ser Tyr Asp Leu Thr Ile Lys Val Gln Asp Gly Gly Ser Pro 
    210                 215                 220 

Pro Arg Ala Thr Ser Ala Leu Leu Arg Val Thr Val Leu Asp Thr Asn 
225                 230                 235                 240 

Asp Asn Ala Pro Lys Phe Glu Arg Pro Ser Tyr Glu Ala Glu Leu Ser 
                245                 250                 255 

Glu Asn Ser Pro Ile Gly His Ser Val Ile Gln Val Lys Ala Asn Asp 
            260                 265                 270 

Ser Asp Gln Gly Ala Asn Ala Glu Ile Glu Tyr Thr Phe His Gln Ala 
        275                 280                 285 

Pro Glu Val Val Arg Arg Leu Leu Arg Leu Asp Arg Asn Thr Gly Leu 
    290                 295                 300 

Ile Thr Val Gln Gly Pro Val Asp Arg Glu Asp Leu Ser Thr Leu Arg 
305                 310                 315                 320 

Phe Ser Val Leu Ala Lys Asp Arg Gly Thr Asn Pro Lys Ser Ala Arg 
                325                 330                 335 

Ala Gln Val Val Val Thr Val Lys Asp Met Asn Asp Asn Ala Pro Thr 
            340                 345                 350 

Ile Glu Ile Arg Gly Ile Gly Leu Val Thr His Gln Asp Gly Met Ala 
        355                 360                 365 

Asn Ile Ser Glu Asp Val Ala Glu Glu Thr Ala Val Ala Leu Val Gln 
    370                 375                 380 

Val Ser Asp Arg Asp Glu Gly Glu Asn Ala Ala Val Thr Cys Val Val 
385                 390                 395                 400 

Ala Gly Asp Val Pro Phe Gln Leu Arg Gln Ala Ser Glu Thr Gly Ser 
                405                 410                 415 

Asp Ser Lys Lys Lys Tyr Phe Leu Gln Thr Thr Thr Pro Leu Asp Tyr 
            420                 425                 430 

Glu Lys Val Lys Asp Tyr Thr Ile Glu Ile Val Ala Val Asp Ser Gly 
        435                 440                 445 

Asn Pro Pro Leu Ser Ser Thr Asn Ser Leu Lys Val Gln Val Val Asp 
    450                 455                 460 

Val Asn Asp Asn Ala Pro Val Phe Thr Gln Ser Val Thr Glu Val Ala 
465                 470                 475                 480 

Phe Pro Glu Asn Asn Lys Pro Gly Glu Val Ile Ala Glu Ile Thr Ala 
                485                 490                 495 

Ser Asp Ala Asp Ser Gly Ser Asn Ala Glu Leu Val Tyr Ser Leu Glu 
            500                 505                 510 

Pro Glu Pro Ala Ala Lys Gly Leu Phe Thr Ile Ser Pro Glu Thr Gly 
        515                 520                 525 

Glu Ile Gln Val Lys Thr Ser Leu Asp Arg Glu Gln Arg Glu Ser Tyr 
    530                 535                 540 

Glu Leu Lys Val Val Ala Ala Asp Arg Gly Ser Pro Ser Leu Gln Gly 
545                 550                 555                 560 

Thr Ala Thr Val Leu Val Asn Val Leu Asp Cys Asn Asp Asn Asp Pro 
                565                 570                 575 

Lys Phe Met Leu Ser Gly Tyr Asn Phe Ser Val Met Glu Asn Met Pro 
            580                 585                 590 

Ala Leu Ser Pro Val Gly Met Val Thr Val Ile Asp Gly Asp Lys Gly 
        595                 600                 605 

Glu Asn Ala Gln Val Gln Leu Ser Val Glu Gln Asp Asn Gly Asp Phe 
    610                 615                 620 

Val Ile Gln Asn Gly Thr Gly Thr Ile Leu Ser Ser Leu Ser Phe Asp 
625                 630                 635                 640 

Arg Glu Gln Gln Ser Thr Tyr Thr Phe Gln Leu Lys Ala Val Asp Gly 
                645                 650                 655 

Gly Val Pro Pro Arg Ser Ala Tyr Val Gly Val Thr Ile Asn Val Leu 
            660                 665                 670 

Asp Glu Asn Asp Asn Ala Pro Tyr Ile Thr Ala Pro Ser Asn Thr Ser 
        675                 680                 685 

His Lys Leu Leu Thr Pro Gln Thr Arg Leu Gly Glu Thr Val Ser Gln 
    690                 695                 700 

Val Ala Ala Glu Asp Phe Asp Ser Gly Val Asn Ala Glu Leu Ile Tyr 
705                 710                 715                 720 

Ser Ile Ala Gly Gly Asn Pro Tyr Gly Leu Phe Gln Ile Gly Ser His 
                725                 730                 735 

Ser Gly Ala Ile Thr Leu Glu Lys Glu Ile Glu Arg Arg His His Gly 
            740                 745                 750 

Leu His Arg Leu Val Val Lys Val Ser Asp Arg Gly Lys Pro Pro Arg 
        755                 760                 765 

Tyr Gly Thr Ala Leu Val His Leu Tyr Val Asn Glu Thr Leu Ala Asn 
    770                 775                 780 

Arg Thr Leu Leu Glu Thr Leu Leu Gly His Ser Leu Asp Thr Pro Leu 
785                 790                 795                 800 

Asp Ile Asp Ile Ala Gly Asp Pro Glu Tyr Glu Arg Ser Lys Gln Arg 
                805                 810                 815 

Gly Asn Ile Leu Phe Gly Val Val Ala Gly Val Val Ala Val Ala Leu 
            820                 825                 830 

Leu Ile Ala Leu Ala Val Leu Val Arg Tyr Cys Arg Gln Arg Glu Ala 
        835                 840                 845 

Lys Ser Gly Tyr Gln Ala Gly Lys Lys Glu Thr Lys Asp Leu Tyr Ala 
    850                 855                 860 

Pro Lys Pro Ser Gly Lys Ala Ser Lys Gly Asn Lys Ser Lys Gly Lys 
865                 870                 875                 880 

Lys Ser Lys Ser Pro Lys Pro Val Lys Pro Val Glu Asp Glu Asp Glu 
                885                 890                 895 

Ala Gly Leu Gln Lys Ser Leu Lys Phe Asn Leu Met Ser Asp Ala Pro 
            900                 905                 910 

Gly Asp Ser Pro Arg Ile His Leu Pro Leu Asn Tyr Pro Pro Gly Ser 
        915                 920                 925 

Pro Asp Leu Gly Arg His Tyr Arg Ser Asn Ser Pro Leu Pro Ser Ile 
    930                 935                 940 

Gln Leu Gln Pro Gln Ser Pro Ser Ala Ser Lys Lys His Gln Val Val 
945                 950                 955                 960 

Gln Asp Leu Pro Pro Ala Asn Thr Phe Val Gly Thr Gly Asp Thr Thr 
                965                 970                 975 

Ser Thr Gly Ser Glu Gln Tyr Ser Asp Tyr Ser Tyr Arg Thr Asn Pro 
            980                 985                 990 

Pro Lys Tyr Pro Ser Lys Gln Leu Pro His Arg Arg Val Thr Phe Ser 
        995                 1000                1005 

Ala Thr Ser Gln Ala Gln Glu Leu Gln Asp Pro Ser Gln His Ser Tyr 
    1010                1015                1020 

Tyr Asp Ser Gly Leu Glu Glu Ser Glu Thr Pro Ser Ser Lys Ser Ser 
1025                1030                1035                1040 

Ser Gly Pro Arg Leu Gly Pro Leu Ala Leu Pro Glu Asp His Tyr Glu 
                1045                1050                1055 

Arg Thr Thr Pro Asp Gly Ser Ile Gly Glu Met Glu His Pro Glu Asn 
            1060                1065                1070 

Asp Leu Arg Pro Leu Pro Asp Val Ala Met Thr Gly Thr Cys Thr Arg 
        1075                1080                1085 

Glu Cys Ser Glu Phe Gly His Ser Asp Thr Cys Trp Met Pro Gly Gln 
    1090                1095                1100 

Ser Ser Pro Ser Arg Arg Thr Lys Ser Ser Ala Leu Lys Leu Ser Thr 
1105                1110                1115                1120 

Phe Met Pro Tyr Gln Asp Arg Gly Gly Gln Glu Pro Ala Gly Ala Gly 
                1125                1130                1135 

Ser Pro Ser Pro Pro Glu Asp Arg Asn Thr Lys Thr Ala Pro Val Arg 
            1140                1145                1150 

Leu Leu Pro Ser Tyr Ser Ala Phe Ser His Ser Ser His Asp Ser Cys 
        1155                1160                1165 

Lys Asp Ser Ala Thr Leu Glu Glu Ile Pro Leu Thr Gln Thr Ser Asp 
    1170                1175                1180 

Phe Pro Pro Ala Ala Thr Pro Ala Ser Ala Gln Thr Ala Lys Arg Glu 
1185                1190                1195                1200 

Ile Tyr Leu 

 
           
           
             
               2789 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
             
               CDS  
                115..2622 

 
             
              104 

CGAAAGCCAT GTCGGACTCG TCGCCCAGCG CCCAAGCGCT AACCCGCTGA AAGTTTCTCA     60 

GCGAAATCTC AGGGACGATC TGGACCCCGC TGAGAGGAAC TGCTTTTGAG TGAG ATG      117 
                                                            Met 
                                                              1 

GTC CCA GAG GCC TGG AGG AGC GGA CTG GTA AGC ACC GGG AGG GTA GTG      165 
Val Pro Glu Ala Trp Arg Ser Gly Leu Val Ser Thr Gly Arg Val Val 
              5                  10                  15 

GGA GTT TTG CTT CTG CTT GGT GCC TTG AAC AAG GCT TCC ACG GTC ATT      213 
Gly Val Leu Leu Leu Leu Gly Ala Leu Asn Lys Ala Ser Thr Val Ile 
         20                  25                  30 

CAC TAT GAG ATC CCG GAG GAA AGA GAG AAG GGT TTC GCT GTG GGC AAC      261 
His Tyr Glu Ile Pro Glu Glu Arg Glu Lys Gly Phe Ala Val Gly Asn 
     35                  40                  45 

GTG GTC GCG AAC CTT GGT TTG GAT CTC GGT AGC CTC TCA GCC CGC AGG      309 
Val Val Ala Asn Leu Gly Leu Asp Leu Gly Ser Leu Ser Ala Arg Arg 
 50                  55                  60                  65 

TTC CCG GTG GTG TCT GGA GCT AGC CGA AGA TTC TTT GAG GTG AAC CGG      357 
Phe Pro Val Val Ser Gly Ala Ser Arg Arg Phe Phe Glu Val Asn Arg 
                 70                  75                  80 

GAG ACC GGA GAG ATG TTT GTG AAC GAC CGT CTG GAT CGA GAG GAG CTG      405 
Glu Thr Gly Glu Met Phe Val Asn Asp Arg Leu Asp Arg Glu Glu Leu 
             85                  90                  95 

TGT GGG ACA CTG CCC TCT TGC ACT GTA ACT CTG GAG TTG GTA GTG GAG      453 
Cys Gly Thr Leu Pro Ser Cys Thr Val Thr Leu Glu Leu Val Val Glu 
        100                 105                 110 

AAC CCG CTG GAG CTG TTC AGC GTG GAA GTG GTG ATC CAG GAC ATC AAC      501 
Asn Pro Leu Glu Leu Phe Ser Val Glu Val Val Ile Gln Asp Ile Asn 
    115                 120                 125 

GAC AAC AAT CCT GCT TTC CCT ACC CAG GAA ATG AAA TTG GAG ATT AGC      549 
Asp Asn Asn Pro Ala Phe Pro Thr Gln Glu Met Lys Leu Glu Ile Ser 
130                 135                 140                 145 

GAG GCC GTG GCT CCG GGG ACG CGC TTT CCG CTC GAG AGC GCG CAC GAT      597 
Glu Ala Val Ala Pro Gly Thr Arg Phe Pro Leu Glu Ser Ala His Asp 
                150                 155                 160 

CCC GAT CTG GGA AGC AAC TCT TTA CAA ACC TAT GAG CTG AGC CGA AAT      645 
Pro Asp Leu Gly Ser Asn Ser Leu Gln Thr Tyr Glu Leu Ser Arg Asn 
            165                 170                 175 

GAA TAC TTT GCG CTT CGC GTG CAG ACG CGG GAG GAC AGC ACC AAG TAC      693 
Glu Tyr Phe Ala Leu Arg Val Gln Thr Arg Glu Asp Ser Thr Lys Tyr 
        180                 185                 190 

GCG GAG CTG GTG TTG GAG CGC GCC CTG GAC CGA GAA CGG GAG CCT AGT      741 
Ala Glu Leu Val Leu Glu Arg Ala Leu Asp Arg Glu Arg Glu Pro Ser 
    195                 200                 205 

CTC CAG TTA GTG CTG ACG GCG TTG GAC GGA GGG ACC CCA GCT CTC TCC      789 
Leu Gln Leu Val Leu Thr Ala Leu Asp Gly Gly Thr Pro Ala Leu Ser 
210                 215                 220                 225 

GCC AGC CTG CCT ATT CAC ATC AAG GTG CTG GAC GCG AAT GAC AAT GCG      837 
Ala Ser Leu Pro Ile His Ile Lys Val Leu Asp Ala Asn Asp Asn Ala 
                230                 235                 240 

CCT GTC TTC AAC CAG TCC TTG TAC CGG GCG CGC GTT CCT GGA GGA TGC      885 
Pro Val Phe Asn Gln Ser Leu Tyr Arg Ala Arg Val Pro Gly Gly Cys 
            245                 250                 255 

ACC TCC GGC ACG CGC GTG GTA CAA GTC CTT GCA ACG GAT CTG GAT GAA      933 
Thr Ser Gly Thr Arg Val Val Gln Val Leu Ala Thr Asp Leu Asp Glu 
        260                 265                 270 

GGC CCC AAC GGT GAA ATT ATT TAC TCC TTC GGC AGC CAC AAC CGC GCC      981 
Gly Pro Asn Gly Glu Ile Ile Tyr Ser Phe Gly Ser His Asn Arg Ala 
    275                 280                 285 

GGC GTG CGG CAA CTA TTC GCC TTA GAC CTT GTA ACC GGG ATG CTG ACA     1029 
Gly Val Arg Gln Leu Phe Ala Leu Asp Leu Val Thr Gly Met Leu Thr 
290                 295                 300                 305 

ATC AAG GGT CGG CTG GAC TTC GAG GAC ACC AAA CTC CAT GAG ATT TAC     1077 
Ile Lys Gly Arg Leu Asp Phe Glu Asp Thr Lys Leu His Glu Ile Tyr 
                310                 315                 320 

ATC CAG GCC AAA GAC AAG GGC GCC AAT CCC GAA GGA GCA CAT TGC AAA     1125 
Ile Gln Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys Lys 
            325                 330                 335 

GTG TTG GTG GAG GTT GTG GAT GTG AAT GAC AAC GCC CCG GAG ATC ACA     1173 
Val Leu Val Glu Val Val Asp Val Asn Asp Asn Ala Pro Glu Ile Thr 
        340                 345                 350 

GTC ACC TCC GTG TAC AGC CCA GTA CCC GAG GAT GCC TCT GGG ACT GTC     1221 
Val Thr Ser Val Tyr Ser Pro Val Pro Glu Asp Ala Ser Gly Thr Val 
    355                 360                 365 

ATC GCT TTG CTC AGT GTG ACT GAC CTG GAT GCT GGC GAG AAC GGG CTG     1269 
Ile Ala Leu Leu Ser Val Thr Asp Leu Asp Ala Gly Glu Asn Gly Leu 
370                 375                 380                 385 

GTG ACC TGC GAA GTT CCA CCG GGT CTC CCT TTC AGC CTT ACT TCT TCC     1317 
Val Thr Cys Glu Val Pro Pro Gly Leu Pro Phe Ser Leu Thr Ser Ser 
                390                 395                 400 

CTC AAG AAT TAC TTC ACT TTG AAA ACC AGT GCA GAC CTG GAT CGG GAG     1365 
Leu Lys Asn Tyr Phe Thr Leu Lys Thr Ser Ala Asp Leu Asp Arg Glu 
            405                 410                 415 

ACT GTG CCA GAA TAC AAC CTC AGC ATC ACC GCC CGA GAC GCC GGA ACC     1413 
Thr Val Pro Glu Tyr Asn Leu Ser Ile Thr Ala Arg Asp Ala Gly Thr 
        420                 425                 430 

CCT TCC CTC TCA GCC CTT ACA ATA GTG CGT GTT CAA GTG TCC GAC ATC     1461 
Pro Ser Leu Ser Ala Leu Thr Ile Val Arg Val Gln Val Ser Asp Ile 
    435                 440                 445 

AAT GAC AAC CCT CCA CAA TCT TCT CAA TCT TCC TAC GAC GTT TAC ATT     1509 
Asn Asp Asn Pro Pro Gln Ser Ser Gln Ser Ser Tyr Asp Val Tyr Ile 
450                 455                 460                 465 

GAA GAA AAC AAC CTC CCC GGG GCT CCA ATA CTA AAC CTA AGT GTC TGG     1557 
Glu Glu Asn Asn Leu Pro Gly Ala Pro Ile Leu Asn Leu Ser Val Trp 
                470                 475                 480 

GAC CCC GAC GCC CCG CAG AAT GCT CGG CTT TCT TTC TTT CTC TTG GAG     1605 
Asp Pro Asp Ala Pro Gln Asn Ala Arg Leu Ser Phe Phe Leu Leu Glu 
            485                 490                 495 

CAA GGA GCT GAA ACC GGG CTA GTG GGT CGC TAT TTC ACA ATA AAT CGT     1653 
Gln Gly Ala Glu Thr Gly Leu Val Gly Arg Tyr Phe Thr Ile Asn Arg 
        500                 505                 510 

GAC AAT GGC ATA GTG TCA TCC TTA GTG CCC CTA GAC TAT GAG GAT CGG     1701 
Asp Asn Gly Ile Val Ser Ser Leu Val Pro Leu Asp Tyr Glu Asp Arg 
    515                 520                 525 

CGG GAA TTT GAA TTA ACA GCT CAT ATC AGC GAT GGG GGC ACC CCG GTC     1749 
Arg Glu Phe Glu Leu Thr Ala His Ile Ser Asp Gly Gly Thr Pro Val 
530                 535                 540                 545 

CTA GCC ACC AAC ATC AGC GTG AAC ATA TTT GTC ACT GAT CGC AAT GAC     1797 
Leu Ala Thr Asn Ile Ser Val Asn Ile Phe Val Thr Asp Arg Asn Asp 
                550                 555                 560 

AAT GCC CCC CAG GTC CTA TAT CCT CGG CCA GGT GGG AGC TCG GTG GAG     1845 
Asn Ala Pro Gln Val Leu Tyr Pro Arg Pro Gly Gly Ser Ser Val Glu 
            565                 570                 575 

ATG CTG CCT CGA GGT ACC TCA GCT GGC CAC CTA GTG TCA CGG GTG GTA     1893 
Met Leu Pro Arg Gly Thr Ser Ala Gly His Leu Val Ser Arg Val Val 
        580                 585                 590 

GGC TGG GAC GCG GAT GCA GGG CAC AAT GCC TGG CTC TCC TAC AGT CTC     1941 
Gly Trp Asp Ala Asp Ala Gly His Asn Ala Trp Leu Ser Tyr Ser Leu 
    595                 600                 605 

TTT GGA TCC CCT AAC CAG AGC CTT TTT GCC ATA GGG CTG CAC ACT GGT     1989 
Phe Gly Ser Pro Asn Gln Ser Leu Phe Ala Ile Gly Leu His Thr Gly 
610                 615                 620                 625 

CAA ATC AGT ACT GCC CGT CCA GTC CAA GAC ACA GAT TCA CCC AGG CAG     2037 
Gln Ile Ser Thr Ala Arg Pro Val Gln Asp Thr Asp Ser Pro Arg Gln 
                630                 635                 640 

ACT CTC ACT GTC TTG ATC AAA GAC AAT GGG GAG CCT TCG CTC TCC ACC     2085 
Thr Leu Thr Val Leu Ile Lys Asp Asn Gly Glu Pro Ser Leu Ser Thr 
            645                 650                 655 

ACT GCT ACC CTC ACT GTG TCA GTA ACC GAG GAC TCT CCT GAA GCC CGA     2133 
Thr Ala Thr Leu Thr Val Ser Val Thr Glu Asp Ser Pro Glu Ala Arg 
        660                 665                 670 

GCC GAG TTC CCC TCT GGC TCT GCC CCC CGG GAG CAG AAA AAA AAT CTC     2181 
Ala Glu Phe Pro Ser Gly Ser Ala Pro Arg Glu Gln Lys Lys Asn Leu 
    675                 680                 685 

ACC TTT TAT CTA CTT CTT TCT CTA ATC CTG GTT TCT GTG GGC TTC GTG     2229 
Thr Phe Tyr Leu Leu Leu Ser Leu Ile Leu Val Ser Val Gly Phe Val 
690                 695                 700                 705 

GTC ACA GTG TTC GGA GTA ATC ATA TTC AAA GTT TAC AAG TGG AAG CAG     2277 
Val Thr Val Phe Gly Val Ile Ile Phe Lys Val Tyr Lys Trp Lys Gln 
                710                 715                 720 

TCT AGA GAC CTA TAC CGA GCC CCG GTG AGC TCA CTG TAC CGA ACA CCA     2325 
Ser Arg Asp Leu Tyr Arg Ala Pro Val Ser Ser Leu Tyr Arg Thr Pro 
            725                 730                 735 

GGG CCC TCC TTG CAC GCG GAC GCC GTG CGG GGA GGC CTG ATG TCG CCG     2373 
Gly Pro Ser Leu His Ala Asp Ala Val Arg Gly Gly Leu Met Ser Pro 
        740                 745                 750 

CAC CTT TAC CAT CAG GTG TAT CTC ACC ACG GAC TCC CGC CGC AGC GAC     2421 
His Leu Tyr His Gln Val Tyr Leu Thr Thr Asp Ser Arg Arg Ser Asp 
    755                 760                 765 

CCG CTG CTG AAG AAA CCT GGT GCA GCC AGT CCA CTG GCC AGC CGC CAG     2469 
Pro Leu Leu Lys Lys Pro Gly Ala Ala Ser Pro Leu Ala Ser Arg Gln 
770                 775                 780                 785 

AAC ACG CTG CGG AGC TGT GAT CCG GTG TTC TAT AGG CAG GTG TTG GGT     2517 
Asn Thr Leu Arg Ser Cys Asp Pro Val Phe Tyr Arg Gln Val Leu Gly 
                790                 795                 800 

GCA GAG AGC GCC CCT CCC GGA CAG GTA AGG TTT AGC AAG TCA TGC TTG     2565 
Ala Glu Ser Ala Pro Pro Gly Gln Val Arg Phe Ser Lys Ser Cys Leu 
            805                 810                 815 

ACC CTG TTA GTG CCT TTT TAT TCC TAC ATC ATA TTG AGA AGG CTG GAG     2613 
Thr Leu Leu Val Pro Phe Tyr Ser Tyr Ile Ile Leu Arg Arg Leu Glu 
        820                 825                 830 

CTG TTT TTT TAGTGATGAA GATGTTTTCC TGGTGATGCA TTCACACTTT             2662 
Leu Phe Phe 
    835 

CAACTGGCTC TTCCTAGATC AAAGTTAGTG CCTTTGTGAG ATGGTGGCCT GCCAGAGTGT   2722 

GGTTTGTGGT CCCATTTCAG GGGGAAGATA CTTGACTCAT CTGTGGACCT AATTCACATC   2782 

CTCAGCG                                                             2789 

 
           
           
             
               836 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              105 

Met Val Pro Glu Ala Trp Arg Ser Gly Leu Val Ser Thr Gly Arg Val 
  1               5                  10                  15 

Val Gly Val Leu Leu Leu Leu Gly Ala Leu Asn Lys Ala Ser Thr Val 
             20                  25                  30 

Ile His Tyr Glu Ile Pro Glu Glu Arg Glu Lys Gly Phe Ala Val Gly 
         35                  40                  45 

Asn Val Val Ala Asn Leu Gly Leu Asp Leu Gly Ser Leu Ser Ala Arg 
     50                  55                  60 

Arg Phe Pro Val Val Ser Gly Ala Ser Arg Arg Phe Phe Glu Val Asn 
 65                  70                  75                  80 

Arg Glu Thr Gly Glu Met Phe Val Asn Asp Arg Leu Asp Arg Glu Glu 
                 85                  90                  95 

Leu Cys Gly Thr Leu Pro Ser Cys Thr Val Thr Leu Glu Leu Val Val 
            100                 105                 110 

Glu Asn Pro Leu Glu Leu Phe Ser Val Glu Val Val Ile Gln Asp Ile 
        115                 120                 125 

Asn Asp Asn Asn Pro Ala Phe Pro Thr Gln Glu Met Lys Leu Glu Ile 
    130                 135                 140 

Ser Glu Ala Val Ala Pro Gly Thr Arg Phe Pro Leu Glu Ser Ala His 
145                 150                 155                 160 

Asp Pro Asp Leu Gly Ser Asn Ser Leu Gln Thr Tyr Glu Leu Ser Arg 
                165                 170                 175 

Asn Glu Tyr Phe Ala Leu Arg Val Gln Thr Arg Glu Asp Ser Thr Lys 
            180                 185                 190 

Tyr Ala Glu Leu Val Leu Glu Arg Ala Leu Asp Arg Glu Arg Glu Pro 
        195                 200                 205 

Ser Leu Gln Leu Val Leu Thr Ala Leu Asp Gly Gly Thr Pro Ala Leu 
    210                 215                 220 

Ser Ala Ser Leu Pro Ile His Ile Lys Val Leu Asp Ala Asn Asp Asn 
225                 230                 235                 240 

Ala Pro Val Phe Asn Gln Ser Leu Tyr Arg Ala Arg Val Pro Gly Gly 
                245                 250                 255 

Cys Thr Ser Gly Thr Arg Val Val Gln Val Leu Ala Thr Asp Leu Asp 
            260                 265                 270 

Glu Gly Pro Asn Gly Glu Ile Ile Tyr Ser Phe Gly Ser His Asn Arg 
        275                 280                 285 

Ala Gly Val Arg Gln Leu Phe Ala Leu Asp Leu Val Thr Gly Met Leu 
    290                 295                 300 

Thr Ile Lys Gly Arg Leu Asp Phe Glu Asp Thr Lys Leu His Glu Ile 
305                 310                 315                 320 

Tyr Ile Gln Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys 
                325                 330                 335 

Lys Val Leu Val Glu Val Val Asp Val Asn Asp Asn Ala Pro Glu Ile 
            340                 345                 350 

Thr Val Thr Ser Val Tyr Ser Pro Val Pro Glu Asp Ala Ser Gly Thr 
        355                 360                 365 

Val Ile Ala Leu Leu Ser Val Thr Asp Leu Asp Ala Gly Glu Asn Gly 
    370                 375                 380 

Leu Val Thr Cys Glu Val Pro Pro Gly Leu Pro Phe Ser Leu Thr Ser 
385                 390                 395                 400 

Ser Leu Lys Asn Tyr Phe Thr Leu Lys Thr Ser Ala Asp Leu Asp Arg 
                405                 410                 415 

Glu Thr Val Pro Glu Tyr Asn Leu Ser Ile Thr Ala Arg Asp Ala Gly 
            420                 425                 430 

Thr Pro Ser Leu Ser Ala Leu Thr Ile Val Arg Val Gln Val Ser Asp 
        435                 440                 445 

Ile Asn Asp Asn Pro Pro Gln Ser Ser Gln Ser Ser Tyr Asp Val Tyr 
    450                 455                 460 

Ile Glu Glu Asn Asn Leu Pro Gly Ala Pro Ile Leu Asn Leu Ser Val 
465                 470                 475                 480 

Trp Asp Pro Asp Ala Pro Gln Asn Ala Arg Leu Ser Phe Phe Leu Leu 
                485                 490                 495 

Glu Gln Gly Ala Glu Thr Gly Leu Val Gly Arg Tyr Phe Thr Ile Asn 
            500                 505                 510 

Arg Asp Asn Gly Ile Val Ser Ser Leu Val Pro Leu Asp Tyr Glu Asp 
        515                 520                 525 

Arg Arg Glu Phe Glu Leu Thr Ala His Ile Ser Asp Gly Gly Thr Pro 
    530                 535                 540 

Val Leu Ala Thr Asn Ile Ser Val Asn Ile Phe Val Thr Asp Arg Asn 
545                 550                 555                 560 

Asp Asn Ala Pro Gln Val Leu Tyr Pro Arg Pro Gly Gly Ser Ser Val 
                565                 570                 575 

Glu Met Leu Pro Arg Gly Thr Ser Ala Gly His Leu Val Ser Arg Val 
            580                 585                 590 

Val Gly Trp Asp Ala Asp Ala Gly His Asn Ala Trp Leu Ser Tyr Ser 
        595                 600                 605 

Leu Phe Gly Ser Pro Asn Gln Ser Leu Phe Ala Ile Gly Leu His Thr 
    610                 615                 620 

Gly Gln Ile Ser Thr Ala Arg Pro Val Gln Asp Thr Asp Ser Pro Arg 
625                 630                 635                 640 

Gln Thr Leu Thr Val Leu Ile Lys Asp Asn Gly Glu Pro Ser Leu Ser 
                645                 650                 655 

Thr Thr Ala Thr Leu Thr Val Ser Val Thr Glu Asp Ser Pro Glu Ala 
            660                 665                 670 

Arg Ala Glu Phe Pro Ser Gly Ser Ala Pro Arg Glu Gln Lys Lys Asn 
        675                 680                 685 

Leu Thr Phe Tyr Leu Leu Leu Ser Leu Ile Leu Val Ser Val Gly Phe 
    690                 695                 700 

Val Val Thr Val Phe Gly Val Ile Ile Phe Lys Val Tyr Lys Trp Lys 
705                 710                 715                 720 

Gln Ser Arg Asp Leu Tyr Arg Ala Pro Val Ser Ser Leu Tyr Arg Thr 
                725                 730                 735 

Pro Gly Pro Ser Leu His Ala Asp Ala Val Arg Gly Gly Leu Met Ser 
            740                 745                 750 

Pro His Leu Tyr His Gln Val Tyr Leu Thr Thr Asp Ser Arg Arg Ser 
        755                 760                 765 

Asp Pro Leu Leu Lys Lys Pro Gly Ala Ala Ser Pro Leu Ala Ser Arg 
    770                 775                 780 

Gln Asn Thr Leu Arg Ser Cys Asp Pro Val Phe Tyr Arg Gln Val Leu 
785                 790                 795                 800 

Gly Ala Glu Ser Ala Pro Pro Gly Gln Val Arg Phe Ser Lys Ser Cys 
                805                 810                 815 

Leu Thr Leu Leu Val Pro Phe Tyr Ser Tyr Ile Ile Leu Arg Arg Leu 
            820                 825                 830 

Glu Leu Phe Phe 
        835 

 
           
           
             
               2751 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
             
               CDS  
                115..2160 

 
             
              106 

CGAAAGCCAT GTCGGACTCG TCGCCCAGCG CCCAAGCGCT AACCCGCTGA AAGTTTCTCA     60 

GCGAAATCTC AGGGACGATC TGGACCCCGC TGAGAGGAAC TGCTTTTGAG TGAG ATG      117 
                                                            Met 
                                                              1 

GTC CCA GAG GCC TGG AGG AGC GGA CTG GTA AGC ACC GGG AGG GTA GTG      165 
Val Pro Glu Ala Trp Arg Ser Gly Leu Val Ser Thr Gly Arg Val Val 
              5                  10                  15 

GGA GTT TTG CTT CTG CTT GGT GCC TTG AAC AAG GCT TCC ACG GTC ATT      213 
Gly Val Leu Leu Leu Leu Gly Ala Leu Asn Lys Ala Ser Thr Val Ile 
         20                  25                  30 

CAC TAT GAG ATC CCG GAG GAA AGA GAG AAG GGT TTC GCT GTG GGC AAC      261 
His Tyr Glu Ile Pro Glu Glu Arg Glu Lys Gly Phe Ala Val Gly Asn 
     35                  40                  45 

GTG GTC GCG AAC CTT GGT TTG GAT CTC GGT AGC CTC TCA GCC CGC AGG      309 
Val Val Ala Asn Leu Gly Leu Asp Leu Gly Ser Leu Ser Ala Arg Arg 
 50                  55                  60                  65 

TTC CCG GTG GTG TCT GGA GCT AGC CGA AGA TTC TTT GAG GTG AAC CGG      357 
Phe Pro Val Val Ser Gly Ala Ser Arg Arg Phe Phe Glu Val Asn Arg 
                 70                  75                  80 

GAG ACC GGA GAG ATG TTT GTG AAC GAC CGT CTG GAT CGA GAG GAG CTG      405 
Glu Thr Gly Glu Met Phe Val Asn Asp Arg Leu Asp Arg Glu Glu Leu 
             85                  90                  95 

TGT GGG ACA CTG CCC TCT TGC ACT GTA ACT CTG GAG TTG GTA GTG GAG      453 
Cys Gly Thr Leu Pro Ser Cys Thr Val Thr Leu Glu Leu Val Val Glu 
        100                 105                 110 

AAC CCG CTG GAG CTG TTC AGC GTG GAA GTG GTG ATC CAG GAC ATC AAC      501 
Asn Pro Leu Glu Leu Phe Ser Val Glu Val Val Ile Gln Asp Ile Asn 
    115                 120                 125 

GAC AAC AAT CCT GCT TTC CCT ACC CAG GAA ATG AAA TTG GAG ATT AGC      549 
Asp Asn Asn Pro Ala Phe Pro Thr Gln Glu Met Lys Leu Glu Ile Ser 
130                 135                 140                 145 

GAG GCC GTG GCT CCG GGG ACG CGC TTT CCG CTC GAG AGC GCG CAC GAT      597 
Glu Ala Val Ala Pro Gly Thr Arg Phe Pro Leu Glu Ser Ala His Asp 
                150                 155                 160 

CCC GAT CTG GGA AGC AAC TCT TTA CAA ACC TAT GAG CTG AGC CGA AAT      645 
Pro Asp Leu Gly Ser Asn Ser Leu Gln Thr Tyr Glu Leu Ser Arg Asn 
            165                 170                 175 

GAA TAC TTT GCG CTT CGC GTG CAG ACG CGG GAG GAC AGC ACC AAG TAC      693 
Glu Tyr Phe Ala Leu Arg Val Gln Thr Arg Glu Asp Ser Thr Lys Tyr 
        180                 185                 190 

GCG GAG CTG GTG TTG GAG CGC GCC CTG GAC CGA GAA CGG GAG CCT AGT      741 
Ala Glu Leu Val Leu Glu Arg Ala Leu Asp Arg Glu Arg Glu Pro Ser 
    195                 200                 205 

CTC CAG TTA GTG CTG ACG GCG TTG GAC GGA GGG ACC CCA GCT CTC TCC      789 
Leu Gln Leu Val Leu Thr Ala Leu Asp Gly Gly Thr Pro Ala Leu Ser 
210                 215                 220                 225 

GCC AGC CTG CCT ATT CAC ATC AAG GTG CTG GAC GCG AAT GAC AAT GCG      837 
Ala Ser Leu Pro Ile His Ile Lys Val Leu Asp Ala Asn Asp Asn Ala 
                230                 235                 240 

CCT GTC TTC AAC CAG TCC TTG TAC CGG GCG CGC GTT CCT GGA GGA TGC      885 
Pro Val Phe Asn Gln Ser Leu Tyr Arg Ala Arg Val Pro Gly Gly Cys 
            245                 250                 255 

ACC TCC GGC ACG CGC GTG GTA CAA GTC CTT GCA ACG GAT CTG GAT GAA      933 
Thr Ser Gly Thr Arg Val Val Gln Val Leu Ala Thr Asp Leu Asp Glu 
        260                 265                 270 

GGC CCC AAC GGT GAA ATT ATT TAC TCC TTC GGC AGC CAC AAC CGC GCC      981 
Gly Pro Asn Gly Glu Ile Ile Tyr Ser Phe Gly Ser His Asn Arg Ala 
    275                 280                 285 

GGC GTG CGG CAA CTA TTC GCC TTA GAC CTT GTA ACC GGG ATG CTG ACA     1029 
Gly Val Arg Gln Leu Phe Ala Leu Asp Leu Val Thr Gly Met Leu Thr 
290                 295                 300                 305 

ATC AAG GGT CGG CTG GAC TTC GAG GAC ACC AAA CTC CAT GAG ATT TAC     1077 
Ile Lys Gly Arg Leu Asp Phe Glu Asp Thr Lys Leu His Glu Ile Tyr 
                310                 315                 320 

ATC CAG GCC AAA GAC AAG GGC GCC AAT CCC GAA GGA GCA CAT TGC AAA     1125 
Ile Gln Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys Lys 
            325                 330                 335 

GTG TTG GTG GAG GTT GTG GAT GTG AAT GAC AAC GCC CCG GAG ATC ACA     1173 
Val Leu Val Glu Val Val Asp Val Asn Asp Asn Ala Pro Glu Ile Thr 
        340                 345                 350 

GTC ACC TCC GTG TAC AGC CCA GTA CCC GAG GAT GCC TCT GGG ACT GTC     1221 
Val Thr Ser Val Tyr Ser Pro Val Pro Glu Asp Ala Ser Gly Thr Val 
    355                 360                 365 

ATC GCT TTG CTC AGT GTG ACT GAC CTG GAT GCT GGC GAG AAC GGG CTG     1269 
Ile Ala Leu Leu Ser Val Thr Asp Leu Asp Ala Gly Glu Asn Gly Leu 
370                 375                 380                 385 

GTG ACC TGC GAA GTT CCA CCG GGT CTC CCT TTC AGC CTT ACT TCT TCC     1317 
Val Thr Cys Glu Val Pro Pro Gly Leu Pro Phe Ser Leu Thr Ser Ser 
                390                 395                 400 

CTC AAG AAT TAC TTC ACT TTG AAA ACC AGT GCA GAC CTG GAT CGG GAG     1365 
Leu Lys Asn Tyr Phe Thr Leu Lys Thr Ser Ala Asp Leu Asp Arg Glu 
            405                 410                 415 

ACT GTG CCA GAA TAC AAC CTC AGC ATC ACC GCC CGA GAC GCC GGA ACC     1413 
Thr Val Pro Glu Tyr Asn Leu Ser Ile Thr Ala Arg Asp Ala Gly Thr 
        420                 425                 430 

CCT TCC CTC TCA GCC CTT ACA ATA GTG CGT GTT CAA GTG TCC GAC ATC     1461 
Pro Ser Leu Ser Ala Leu Thr Ile Val Arg Val Gln Val Ser Asp Ile 
    435                 440                 445 

AAT GAC AAC CCT CCA CAA TCT TCT CAA TCT TCC TAC GAC GTT TAC ATT     1509 
Asn Asp Asn Pro Pro Gln Ser Ser Gln Ser Ser Tyr Asp Val Tyr Ile 
450                 455                 460                 465 

GAA GAA AAC AAC CTC CCC GGG GCT CCA ATA CTA AAC CTA AGT GTC TGG     1557 
Glu Glu Asn Asn Leu Pro Gly Ala Pro Ile Leu Asn Leu Ser Val Trp 
                470                 475                 480 

GAC CCC GAC GCC CCG CAG AAT GCT CGG CTT TCT TTC TTT CTC TTG GAG     1605 
Asp Pro Asp Ala Pro Gln Asn Ala Arg Leu Ser Phe Phe Leu Leu Glu 
            485                 490                 495 

CAA GGA GCT GAA ACC GGG CTA GTG GGT CGC TAT TTC ACA ATA AAT CGT     1653 
Gln Gly Ala Glu Thr Gly Leu Val Gly Arg Tyr Phe Thr Ile Asn Arg 
        500                 505                 510 

GAC AAT GGC ATA GTG TCA TCC TTA GTG CCC CTA GAC TAT GAG GAT CGG     1701 
Asp Asn Gly Ile Val Ser Ser Leu Val Pro Leu Asp Tyr Glu Asp Arg 
    515                 520                 525 

CGG GAA TTT GAA TTA ACA GCT CAT ATC AGC GAT GGG GGC ACC CCG GTC     1749 
Arg Glu Phe Glu Leu Thr Ala His Ile Ser Asp Gly Gly Thr Pro Val 
530                 535                 540                 545 

CTA GCC ACC AAC ATC AGC GTG AAC ATA TTT GTC ACT GAT CGC AAT GAC     1797 
Leu Ala Thr Asn Ile Ser Val Asn Ile Phe Val Thr Asp Arg Asn Asp 
                550                 555                 560 

AAT GCC CCC CAG GTC CTA TAT CCT CGG CCA GGT GGG AGC TCG GTG GAG     1845 
Asn Ala Pro Gln Val Leu Tyr Pro Arg Pro Gly Gly Ser Ser Val Glu 
            565                 570                 575 

ATG CTG CCT CGA GGT ACC TCA GCT GGC CAC CTA GTG TCA CGG GTG GTA     1893 
Met Leu Pro Arg Gly Thr Ser Ala Gly His Leu Val Ser Arg Val Val 
        580                 585                 590 

GGC TGG GAC GCG GAT GCA GGG CAC AAT GCC TGG CTC TCC TAC AGT CTC     1941 
Gly Trp Asp Ala Asp Ala Gly His Asn Ala Trp Leu Ser Tyr Ser Leu 
    595                 600                 605 

TTT GGA TCC CCT AAC CAG AGC CTT TTT GCC ATA GGG CTG CAC ACT GGT     1989 
Phe Gly Ser Pro Asn Gln Ser Leu Phe Ala Ile Gly Leu His Thr Gly 
610                 615                 620                 625 

CAA ATC AGT ACT GCC CGT CCA GTC CAA GAC ACA GAT TCA CCC AGG CAG     2037 
Gln Ile Ser Thr Ala Arg Pro Val Gln Asp Thr Asp Ser Pro Arg Gln 
                630                 635                 640 

ACT CTC ACT GTC TTG ATC AAA GAC AAT GGG GAG CCT TCG CTC TCC ACC     2085 
Thr Leu Thr Val Leu Ile Lys Asp Asn Gly Glu Pro Ser Leu Ser Thr 
            645                 650                 655 

ACT GCT ACC CTC ACT GTG TCA GTA ACC GAG GAC TCT CCT GAA GCC CGA     2133 
Thr Ala Thr Leu Thr Val Ser Val Thr Glu Asp Ser Pro Glu Ala Arg 
        660                 665                 670 

GCC GAG TTC CCC TCT GGC TCT GCC AGT TAAACCTTCT TTAATTATGG           2180 
Ala Glu Phe Pro Ser Gly Ser Ala Ser 
    675                 680 

ATTAGCCATT AACATTTTTG AAACGTGGAC CATTTAACCT CGGCCTACCC CCTCCAACTG   2240 

TCCTGGTGAT GAGTTCATTA GCTAAGTTAA ATTAATTGAA CTTTGATCTA AACCAAAACA   2300 

AATCAGGAAA ATAAAGCTGT AAAGGAACTT ATCAAGCATT CCAAAACCAA CTAGAAATTA   2360 

CTTGAAGTTT CGAGTGAGCA TTGCCTGTGC CAGTATTCTT CATTATAGGA TTATAAACTC   2420 

GTTTTTTTCC CAAAGCGCAT GTCTACGCCA GGCAGAGGAG TAATTATTCA GCCAATTTCA   2480 

TGGATGTAAC GATGGATATA AATAATTGAT AGCACCTAGA GGCTTCCAGT TTGGGTGGAA   2540 

GGCTAAAAGT AGAGGGGAAC TCACTCACTT GAGAAATGAT ATTTAAGTGA ATAAATAGTT   2600 

CTCTTCTATG AAACTATTAC TATTTAGTTC TCTGGAAAAC TTAAGTGTAT TAATGATTAG   2660 

AACATCAAAT CCTAAGTAAA GAAATGACAT TTTAAATATA AAAAGCCAAA CTTTAAATAA   2720 

ATCATAGAGA CCTCAGACAT AATATAGGAA A                                  2751 

 
           
           
             
               682 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              107 

Met Val Pro Glu Ala Trp Arg Ser Gly Leu Val Ser Thr Gly Arg Val 
  1               5                  10                  15 

Val Gly Val Leu Leu Leu Leu Gly Ala Leu Asn Lys Ala Ser Thr Val 
             20                  25                  30 

Ile His Tyr Glu Ile Pro Glu Glu Arg Glu Lys Gly Phe Ala Val Gly 
         35                  40                  45 

Asn Val Val Ala Asn Leu Gly Leu Asp Leu Gly Ser Leu Ser Ala Arg 
     50                  55                  60 

Arg Phe Pro Val Val Ser Gly Ala Ser Arg Arg Phe Phe Glu Val Asn 
 65                  70                  75                  80 

Arg Glu Thr Gly Glu Met Phe Val Asn Asp Arg Leu Asp Arg Glu Glu 
                 85                  90                  95 

Leu Cys Gly Thr Leu Pro Ser Cys Thr Val Thr Leu Glu Leu Val Val 
            100                 105                 110 

Glu Asn Pro Leu Glu Leu Phe Ser Val Glu Val Val Ile Gln Asp Ile 
        115                 120                 125 

Asn Asp Asn Asn Pro Ala Phe Pro Thr Gln Glu Met Lys Leu Glu Ile 
    130                 135                 140 

Ser Glu Ala Val Ala Pro Gly Thr Arg Phe Pro Leu Glu Ser Ala His 
145                 150                 155                 160 

Asp Pro Asp Leu Gly Ser Asn Ser Leu Gln Thr Tyr Glu Leu Ser Arg 
                165                 170                 175 

Asn Glu Tyr Phe Ala Leu Arg Val Gln Thr Arg Glu Asp Ser Thr Lys 
            180                 185                 190 

Tyr Ala Glu Leu Val Leu Glu Arg Ala Leu Asp Arg Glu Arg Glu Pro 
        195                 200                 205 

Ser Leu Gln Leu Val Leu Thr Ala Leu Asp Gly Gly Thr Pro Ala Leu 
    210                 215                 220 

Ser Ala Ser Leu Pro Ile His Ile Lys Val Leu Asp Ala Asn Asp Asn 
225                 230                 235                 240 

Ala Pro Val Phe Asn Gln Ser Leu Tyr Arg Ala Arg Val Pro Gly Gly 
                245                 250                 255 

Cys Thr Ser Gly Thr Arg Val Val Gln Val Leu Ala Thr Asp Leu Asp 
            260                 265                 270 

Glu Gly Pro Asn Gly Glu Ile Ile Tyr Ser Phe Gly Ser His Asn Arg 
        275                 280                 285 

Ala Gly Val Arg Gln Leu Phe Ala Leu Asp Leu Val Thr Gly Met Leu 
    290                 295                 300 

Thr Ile Lys Gly Arg Leu Asp Phe Glu Asp Thr Lys Leu His Glu Ile 
305                 310                 315                 320 

Tyr Ile Gln Ala Lys Asp Lys Gly Ala Asn Pro Glu Gly Ala His Cys 
                325                 330                 335 

Lys Val Leu Val Glu Val Val Asp Val Asn Asp Asn Ala Pro Glu Ile 
            340                 345                 350 

Thr Val Thr Ser Val Tyr Ser Pro Val Pro Glu Asp Ala Ser Gly Thr 
        355                 360                 365 

Val Ile Ala Leu Leu Ser Val Thr Asp Leu Asp Ala Gly Glu Asn Gly 
    370                 375                 380 

Leu Val Thr Cys Glu Val Pro Pro Gly Leu Pro Phe Ser Leu Thr Ser 
385                 390                 395                 400 

Ser Leu Lys Asn Tyr Phe Thr Leu Lys Thr Ser Ala Asp Leu Asp Arg 
                405                 410                 415 

Glu Thr Val Pro Glu Tyr Asn Leu Ser Ile Thr Ala Arg Asp Ala Gly 
            420                 425                 430 

Thr Pro Ser Leu Ser Ala Leu Thr Ile Val Arg Val Gln Val Ser Asp 
        435                 440                 445 

Ile Asn Asp Asn Pro Pro Gln Ser Ser Gln Ser Ser Tyr Asp Val Tyr 
    450                 455                 460 

Ile Glu Glu Asn Asn Leu Pro Gly Ala Pro Ile Leu Asn Leu Ser Val 
465                 470                 475                 480 

Trp Asp Pro Asp Ala Pro Gln Asn Ala Arg Leu Ser Phe Phe Leu Leu 
                485                 490                 495 

Glu Gln Gly Ala Glu Thr Gly Leu Val Gly Arg Tyr Phe Thr Ile Asn 
            500                 505                 510 

Arg Asp Asn Gly Ile Val Ser Ser Leu Val Pro Leu Asp Tyr Glu Asp 
        515                 520                 525 

Arg Arg Glu Phe Glu Leu Thr Ala His Ile Ser Asp Gly Gly Thr Pro 
    530                 535                 540 

Val Leu Ala Thr Asn Ile Ser Val Asn Ile Phe Val Thr Asp Arg Asn 
545                 550                 555                 560 

Asp Asn Ala Pro Gln Val Leu Tyr Pro Arg Pro Gly Gly Ser Ser Val 
                565                 570                 575 

Glu Met Leu Pro Arg Gly Thr Ser Ala Gly His Leu Val Ser Arg Val 
            580                 585                 590 

Val Gly Trp Asp Ala Asp Ala Gly His Asn Ala Trp Leu Ser Tyr Ser 
        595                 600                 605 

Leu Phe Gly Ser Pro Asn Gln Ser Leu Phe Ala Ile Gly Leu His Thr 
    610                 615                 620 

Gly Gln Ile Ser Thr Ala Arg Pro Val Gln Asp Thr Asp Ser Pro Arg 
625                 630                 635                 640 

Gln Thr Leu Thr Val Leu Ile Lys Asp Asn Gly Glu Pro Ser Leu Ser 
                645                 650                 655 

Thr Thr Ala Thr Leu Thr Val Ser Val Thr Glu Asp Ser Pro Glu Ala 
            660                 665                 670 

Arg Ala Glu Phe Pro Ser Gly Ser Ala Ser 
        675                 680 

 
           
           
             
               2831 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              108 

GAATTCGGCA CGAGGCTGAA CTGAGGGTGA CGGACATAAA CGACTATTCT CCAGTGTTCA     60 

GTGAAAGAGA AATGATACTG AGGATACCAG AAAACAGTGC TCGGGGAAAT ACATTCCCTT    120 

TAAACAATGC TCTGGACTCA GACGTAGATA TCAACAATAT CCAGACCTAT AGGCTCAGCT    180 

CAAACTCTCA TTTCCTGGTT GTAACCCGCA ACCGCAGTGA TGGCAGGAAG TACCCAGAGC    240 

TGGTGCTGGA GAAAGAACTG GATCGAGAGG AGGAACCTGA GCTGAGGTTA ACGCTGACAG    300 

CTTTGGATGG TGGCTCTCCT CCCCGGTCTG GGACGACACA GGTCCTCATT GAAGTAGTGG    360 

ACACCAACGA TAATGCACCC GAGTTTCAGC AGCCAACATA CCAAGTGCAA ACTCCCGAGA    420 

ACAGTCCCAC CGGCTCTCTG GTACTCACAG TCTCAGCCAA TGACTTAGAC AGTGGAGACT    480 

ATGGGAAAGT CTTGTACGCA CTTTCGCAAC CCTCAGAAGA TATTAGCAAA ACATTCGAGG    540 

TAAACCCTGT AACCGGGGAA ATTCGCCTAC GAAAAGAGGT GAATTTTGAA ACTATTCCTT    600 

CGTATGAAGT GGTTATCAAG GGGACGGACG GGGGAGGTCT CTCAGGAAAA TGCACTCTGT    660 

TACTGCAGGT GGTGGACGTG AATGACAATG CCCCAGAAGT GATGCTATCT GCGCTAACCA    720 

ACCCAGTCCC AGAAAATTCC CCCGATGAGG TAGTGGCTGT TTTCAGTGTT AGAGATCCTG    780 

ACTCTGGGAA CAACGGAAAA GTGATTGCAT CCATCGAGGA AGACCTGCCC TTTCTTCTAA    840 

AATCTTCAGG AAAGAACTTT TACACTTTAG TAACCAAGGG AGCACTTGAC AGGGAAGAAA    900 

GAGAGCAATT GAACATCACC ATCACAGTCA CTGACCTGGG CATACCCAGG CTCACCACCC    960 

AACACACCAT AACAGTGCAG GTGGCAGACA TCAACGACAA TGCCCCCTCC TTCACCCAAA   1020 

CCTCCTACAC CATGTTTGTC CGCGAGAACA ACAGCCCCGC CCTGCACATA GGCACCATCA   1080 

GCGCCACAGA CTCAGACTCA GGATCCAATG CCCACATCAC CTACTCGCTG CTACCGCCCC   1140 

AAGACCCACA GCTGGCCCTC GACTCGCTCA TCTCCATCAA TGTAGACAAC GGGCAGCTGT   1200 

TCGCGCTCAG GGCGCTAGAC TATGAGGCTC TGCAGGGCTT CGAGTTCCAT GTGGGCGCCA   1260 

CAGACCAAGG CTCGCCCGCG CTCAGCAGCC AGGCTCTGGT GCACGTGGTG GTGTTGGACG   1320 

ACAATGACAA TGCGCCCTTC GTGCTCTACC CGCTGCAAAA CGCCTCTGCA CCCTTCACTG   1380 

AGCTGCTGCC CAGGGCGGCA GAGCCTGGAT ACCTGGTTAC CAAGGTGGTA GCTGTGGACC   1440 

GCGACTCTGG CCAGAATGCC TGGCTGTCAT TCCAGCTGCT CAAGGCCACG GAGCCCGGGC   1500 

TGTTCAACGT ATGGGCGCAC AATGGCGAGG TACGCACCTC CAGGCTGCTG AGCGAGCGCG   1560 

ACGCACCCAA GCACAAGCTG CTGCTGTTGG TCAAGGACAA TGGAGATCCT CCACGCTCTG   1620 

CCAGTGTTAC TCTGCACGTG CTAGTGGTGG ATGCCTTCTC TCAGCCCTAC CTGCCTCTGC   1680 

CAGAGGTGGC GCACGACCCT GCACAAGAAG AAGATGCGCT AACACTCTAC CTGGTCATAG   1740 

CTTTGGCATC TGTGTCTTCT CTCTTCCTCT TGTCTGTGCT GCTGTTCGTG GGGGTGAGGC   1800 

TCTGCAGGAG GGCCAGGGCA GCCTCTCTGA GTGCCTATTC TGTGCCTGAA GGCCACTTTC   1860 

CTGGCCAGCT GGTGGATGTC AGAGGTATGG GGACCCTGTC CCAGAGCTAC CAGTATGATG   1920 

TATGTCTGAT GGGGGATTCT TCTGGGACCA GCGAATTTAA CTTCTTAAAG CCAGTTCTGC   1980 

CTAGCTCTCT GCACCAGTGC TCTGGGAAAG AAATAGAGGA AAATTCCACA CTCCAGAATA   2040 

GTTTTGGGTT TCATCATTAA TAGAAAACTA CTTTACAGAT ATTTAATTCC AAATATCATC   2100 

TTGTTGATTA ACTAAAGTCT GTTCACATGT AGCTAGCTAG CAACGATTTT AATGTTCACT   2160 

TTACCCATCT TTTTTCAGGG TCATGTCTAA AGCTACAAGT TTGNCTTTAC TTATACTTGT   2220 

CGCACAGAAT NNNNNNNNNN TGGTGTATAA GTCACAGTCA TGGGATACTG GCACAAGATG   2280 

GCAGCTTGAT TGCTCAGTTA TGGCTGCAAA GGGGNGCTTG AGTTTAGGGA ATGTGTTAGA   2340 

GCTGGAATAA GTTTTCTGAG AAATGTGTAA GACAAATTTC TTTTGCACAT TCCCTGTGTT   2400 

CCTGTACCCC TGTTTCCAGA ACTACGAAAT GTGTCATCAG AAGGCATGCT CACATTTTCC   2460 

CCTTTGTTTG CGTGACCCGG GTGCCAGAAA TTAAATAAAA TTAGCATGGA GTTCAATGCA   2520 

GCATTAAAAC AAAGTTACTT CTACAAACCT TTTATTCGAC GGTTAAAATT GTAACTTCCC   2580 

CACCCATGAG GCTGGCTGTA AGAACCAGTA TGAATGGGTG TCTATCGCAA CCTTATTTCC   2640 

AAAAATCAAA CAAAAGGAGA AATGAGAGAC CAAACAACAC GCTACAGGAA AGATTTCATA   2700 

AGGATGTATG TATGGACACA AAAACTGGGA TACAGACATT TTAAATCTGT TGGTACCACA   2760 

TGGTGGCGCT GCAGGCTAAA GAAATGCAAG GGAAATTAAA AAGAGGCTGA GCTAGAAGTC   2820 

AAAAAAAAAA A                                                        2831 

 
           
           
             
               3353 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
             
               CDS  
                763..3123 

 
             
              109 

GTATTTTTCC ACAGTTTAAA ATTTTCATAA AATCATAACT CTCTGACTTT ATGTAGAAAG     60 

GATACCACAC TGGAATTAAC GTGTAGCTTT TTCTTGATGT AATCCAACCA ATGGGAGCAC    120 

AATTCTGGTA CATAGGCTGT CTAGAATTTG AAAGAAATTA AAGAATTCAT TTTGTTTTGC    180 

TGATAAATTT TTAAGAAATC ACGTGGCTTT ATGTTATTAT TATTACAAGA TGACTGATCA    240 

CTATTATGTC TTCTTTCACT TCTCAATTTC CCTCAGAACA CTACACCCAG ACTACAGGCT    300 

CTGGAGGGTG GGGACCATGT CTGGGTTGTT TACTGATGTA TTTCATAATT TGGCACATAG    360 

AGACCAATAA TACTCCTTTA AATGAAGAAA TTAATAATTA CCATTGCGTG ATATTGTGAT    420 

TACATCATTT CCTCCCAATT TCCAAACTCC TAATAGAATA GAGAATAGAT CAATTGTAGC    480 

AATTCGTTTC GAAGCAAAGA CAACGCATGG TGGCGCTGCA GGCTAAGGCT TCAAAAAAAG    540 

GAAAAGGAAA AAGCCCATGA AATGCTACTA GCTACTTCAG ACCTCTTTCA GCCTAAGAGG    600 

AAAGCCTGTT AGCAGAGCAC GGACCAGTGT CTCCGGAGAA TGCTATTCTC CTACATTTCC    660 

GAACAGGTTA TCAACGCACA GATCGATCAC TGCCTCTGTC CCATCGCTCC CTGAAGTAGC    720 

TCTGACTCCG GTTCCTTGAA AGGGGCGTGT ACAGAAGTAA AG ATG GAG CCT GCA       774 
                                               Met Glu Pro Ala 
                                                 1 

GGG GAG CGC TTT CCC GAA CAA AGG CAA GTC CTG ATT CTC CTT CTT TTA      822 
Gly Glu Arg Phe Pro Glu Gln Arg Gln Val Leu Ile Leu Leu Leu Leu 
  5                  10                  15                  20 

CTG GAA GTG ACT CTG GCA GGC TGG GAA CCC CGT CGC TAT TCT GTG ATG      870 
Leu Glu Val Thr Leu Ala Gly Trp Glu Pro Arg Arg Tyr Ser Val Met 
                 25                  30                  35 

GAG GAA ACA GAG AGA GGT TCT TTT GTA GCC AAC CTG GCC AAT GAC CTA      918 
Glu Glu Thr Glu Arg Gly Ser Phe Val Ala Asn Leu Ala Asn Asp Leu 
             40                  45                  50 

GGG CTG GGA GTG GGG GAG CTA GCC GAG CGG GGA GCC CGG GTA GTT TCT      966 
Gly Leu Gly Val Gly Glu Leu Ala Glu Arg Gly Ala Arg Val Val Ser 
         55                  60                  65 

GAG GAT AAC GAA CAA GGC TTG CAG CTT GAT CTG CAG ACC GGG CAG TTG     1014 
Glu Asp Asn Glu Gln Gly Leu Gln Leu Asp Leu Gln Thr Gly Gln Leu 
     70                  75                  80 

ATA TTA AAT GAG AAG CTG GAC CGG GAG AAG CTG TGT GGC CCT ACT GAG     1062 
Ile Leu Asn Glu Lys Leu Asp Arg Glu Lys Leu Cys Gly Pro Thr Glu 
 85                  90                  95                 100 

CCC TGT ATA ATG CAT TTC CAA GTG TTA CTG AAA AAA CCT TTG GAA GTA     1110 
Pro Cys Ile Met His Phe Gln Val Leu Leu Lys Lys Pro Leu Glu Val 
                105                 110                 115 

TTT CGA GCT GAA CTA CTA GTG ACA GAC ATA AAC GAT CAT TCT CCT GAG     1158 
Phe Arg Ala Glu Leu Leu Val Thr Asp Ile Asn Asp His Ser Pro Glu 
            120                 125                 130 

TTT CCT GAA AGA GAA ATG ACC CTG AAA ATC CCA GAA ACT AGC TCC CTT     1206 
Phe Pro Glu Arg Glu Met Thr Leu Lys Ile Pro Glu Thr Ser Ser Leu 
        135                 140                 145 

GGG ACT GTG TTT CCT CTG AAA AAA GCT CGG GAC TTG GAC GTG GGC AGC     1254 
Gly Thr Val Phe Pro Leu Lys Lys Ala Arg Asp Leu Asp Val Gly Ser 
    150                 155                 160 

AAT AAT GTT CAA AAC TAC AAT ATT TCT CCC AAT TCT CAT TTC CAT GTT     1302 
Asn Asn Val Gln Asn Tyr Asn Ile Ser Pro Asn Ser His Phe His Val 
165                 170                 175                 180 

TCC ACT CGC ACC CGA GGG GAT GGC AGG AAA TAC CCA GAG CTG GTG CTG     1350 
Ser Thr Arg Thr Arg Gly Asp Gly Arg Lys Tyr Pro Glu Leu Val Leu 
                185                 190                 195 

GAC ACA GAA CTG GAT CGC GAG GAG CAG GCC GAG CTC AGA TTA ACC TTG     1398 
Asp Thr Glu Leu Asp Arg Glu Glu Gln Ala Glu Leu Arg Leu Thr Leu 
            200                 205                 210 

ACA GCG GTG GAC GGT GGC TCT CCA CCC CGA TCT GGC ACC GTC CAG ATC     1446 
Thr Ala Val Asp Gly Gly Ser Pro Pro Arg Ser Gly Thr Val Gln Ile 
        215                 220                 225 

CTC ATC TTG GTC TTG GAC GCC AAT GAC AAT GCC CCG GAG TTT GTG CAG     1494 
Leu Ile Leu Val Leu Asp Ala Asn Asp Asn Ala Pro Glu Phe Val Gln 
    230                 235                 240 

GCG CTC TAC GAG GTG CAG GTC CCA GAG AAC AGC CCA GTA GGC TCC CTA     1542 
Ala Leu Tyr Glu Val Gln Val Pro Glu Asn Ser Pro Val Gly Ser Leu 
245                 250                 255                 260 

GTT GTC AAG GTC TCT GCT AGG GAT TTA GAC ACT GGG ACA AAT GGA GAG     1590 
Val Val Lys Val Ser Ala Arg Asp Leu Asp Thr Gly Thr Asn Gly Glu 
                265                 270                 275 

ATA TCA TAC TCC CTT TAT TAC AGC TCT CAG GAG ATA GAC AAA CCT TTT     1638 
Ile Ser Tyr Ser Leu Tyr Tyr Ser Ser Gln Glu Ile Asp Lys Pro Phe 
            280                 285                 290 

GAG CTA AGC AGC CTT TCA GGA GAA ATT CGA CTA ATT AAA AAA CTA GAT     1686 
Glu Leu Ser Ser Leu Ser Gly Glu Ile Arg Leu Ile Lys Lys Leu Asp 
        295                 300                 305 

TTT GAG ACA ATG TCT TCA TAT GAT CTA GAT ATA GAG GCA TCT GAT GGC     1734 
Phe Glu Thr Met Ser Ser Tyr Asp Leu Asp Ile Glu Ala Ser Asp Gly 
    310                 315                 320 

GGG GGA CTT TCT GGA AAA TGC TCT GTC TCT GTT AAG GTG CTG GAT GTT     1782 
Gly Gly Leu Ser Gly Lys Cys Ser Val Ser Val Lys Val Leu Asp Val 
325                 330                 335                 340 

AAC GAT AAC TTC CCG GAA CTA AGT ATT TCA TCA CTT ACC AGC CCT ATT     1830 
Asn Asp Asn Phe Pro Glu Leu Ser Ile Ser Ser Leu Thr Ser Pro Ile 
                345                 350                 355 

CCC GAG AAT TCT CCA GAG ACA GAA GTG GCC CTG TTT AGG ATT AGA GAC     1878 
Pro Glu Asn Ser Pro Glu Thr Glu Val Ala Leu Phe Arg Ile Arg Asp 
            360                 365                 370 

CGA GAC TCT GGA GAA AAT GGA AAA ATG ATT TGC TCA ATT CAG GAT GAT     1926 
Arg Asp Ser Gly Glu Asn Gly Lys Met Ile Cys Ser Ile Gln Asp Asp 
        375                 380                 385 

GTT CCT TTT AAG CTA AAA CCT TCT GTT GAG AAT TTC TAC AGG CTG GTA     1974 
Val Pro Phe Lys Leu Lys Pro Ser Val Glu Asn Phe Tyr Arg Leu Val 
    390                 395                 400 

ACA GAA GGG GCG CTG GAC AGA GAG ACC AGA GCC GAG TAC AAC ATC ACC     2022 
Thr Glu Gly Ala Leu Asp Arg Glu Thr Arg Ala Glu Tyr Asn Ile Thr 
405                 410                 415                 420 

ATC ACC ATC ACA GAC TTG GGG ACT CCA AGG CTG AAA ACC GAG CAG AGC     2070 
Ile Thr Ile Thr Asp Leu Gly Thr Pro Arg Leu Lys Thr Glu Gln Ser 
                425                 430                 435 

ATA ACC GTG CTG GTG TCG GAC GTC AAT GAC AAC GCC CCC GCC TTC ACC     2118 
Ile Thr Val Leu Val Ser Asp Val Asn Asp Asn Ala Pro Ala Phe Thr 
            440                 445                 450 

CAA ACC TCC TAC ACC CTG TTC GTC CGC GAG AAC AAC AGC CCC GCC CTG     2166 
Gln Thr Ser Tyr Thr Leu Phe Val Arg Glu Asn Asn Ser Pro Ala Leu 
        455                 460                 465 

CAC ATC GGC AGT GTC AGC GCC ACA GAC AGA GAC TCG GGC ACC AAC GCC     2214 
His Ile Gly Ser Val Ser Ala Thr Asp Arg Asp Ser Gly Thr Asn Ala 
    470                 475                 480 

CAG GTC ACC TAC TCG CTG CTG CCG CCC CAG GAC CCG CAC CTG CCC CTA     2262 
Gln Val Thr Tyr Ser Leu Leu Pro Pro Gln Asp Pro His Leu Pro Leu 
485                 490                 495                 500 

ACC TCC CTG GTC TCC ATT AAC ACG GAC AAC GGC CAC CTG TTC GCT CTC     2310 
Thr Ser Leu Val Ser Ile Asn Thr Asp Asn Gly His Leu Phe Ala Leu 
                505                 510                 515 

CAG TCG CTG GAC TAC GAG GCC CTG CAG GCT TTC GAG TTC CGC GTG GGC     2358 
Gln Ser Leu Asp Tyr Glu Ala Leu Gln Ala Phe Glu Phe Arg Val Gly 
            520                 525                 530 

GCC ACA GAC CGC GGC TTC CCG GCG CTG AGC AGC GAG GCG CTG GTG CGA     2406 
Ala Thr Asp Arg Gly Phe Pro Ala Leu Ser Ser Glu Ala Leu Val Arg 
        535                 540                 545 

GTG CTG GTG CTG GAC GCC AAC GAC AAC TCG CCC TTC GTG CTG TAC CCG     2454 
Val Leu Val Leu Asp Ala Asn Asp Asn Ser Pro Phe Val Leu Tyr Pro 
    550                 555                 560 

CTG CAG AAC GGC TCC GCG CCC TGC ACC GAG CTG GTG CCC CGG GCG GCC     2502 
Leu Gln Asn Gly Ser Ala Pro Cys Thr Glu Leu Val Pro Arg Ala Ala 
565                 570                 575                 580 

GAG CCG GGC TAC CTG GTG ACC AAG GTG GTG GCG GTG GAC GGC GAC TCG     2550 
Glu Pro Gly Tyr Leu Val Thr Lys Val Val Ala Val Asp Gly Asp Ser 
                585                 590                 595 

GGC CAG AAC GCC TGG CTG TCG TAC CAG CTG CTC AAG GCC ACG GAG CCC     2598 
Gly Gln Asn Ala Trp Leu Ser Tyr Gln Leu Leu Lys Ala Thr Glu Pro 
            600                 605                 610 

GGG CTG TTC GGC GTG TGG GCG CAC AAT GGC GAG GTG CGC ACC GCC AGG     2646 
Gly Leu Phe Gly Val Trp Ala His Asn Gly Glu Val Arg Thr Ala Arg 
        615                 620                 625 

CTG CTG AGC GAG CGC GAC GTG GCC AAG CAC AGG CTA GTG GTG CTG GTC     2694 
Leu Leu Ser Glu Arg Asp Val Ala Lys His Arg Leu Val Val Leu Val 
    630                 635                 640 

AAG GAC AAT GGC GAG CCT CCG CGC TCG GCC ACA GCC ACG CTG CAA GTG     2742 
Lys Asp Asn Gly Glu Pro Pro Arg Ser Ala Thr Ala Thr Leu Gln Val 
645                 650                 655                 660 

CTC CTG GTG GAC GGC TTC TCT CAG CCC TAC CTG CCG CTC CCA GAG GCG     2790 
Leu Leu Val Asp Gly Phe Ser Gln Pro Tyr Leu Pro Leu Pro Glu Ala 
                665                 670                 675 

GCC CCG GCC CAA GCC CAG GCC GAC TCG CTT ACC GTC TAC CTG GTG GTG     2838 
Ala Pro Ala Gln Ala Gln Ala Asp Ser Leu Thr Val Tyr Leu Val Val 
            680                 685                 690 

GCA TTG GCC TCG GTG TCT TCG CTC TTC CTC TTC TCG GTG TTC CTG TTC     2886 
Ala Leu Ala Ser Val Ser Ser Leu Phe Leu Phe Ser Val Phe Leu Phe 
        695                 700                 705 

GTG GCA GTG CGG CTG TGC AGG AGG AGC AGG GCG GCC TCA GTG GGT CGC     2934 
Val Ala Val Arg Leu Cys Arg Arg Ser Arg Ala Ala Ser Val Gly Arg 
    710                 715                 720 

TGC TCG GTG CCC GAG GGC CCC TTT CCA GGG CAT CTG GTG GAC GTG AGC     2982 
Cys Ser Val Pro Glu Gly Pro Phe Pro Gly His Leu Val Asp Val Ser 
725                 730                 735                 740 

GGC ACC GGG ACC CTT TCC CAG AGC TAC CAG TAC GAG GTG TGT CTG ACG     3030 
Gly Thr Gly Thr Leu Ser Gln Ser Tyr Gln Tyr Glu Val Cys Leu Thr 
                745                 750                 755 

GGA GGC TCT GAA AGT AAT GAT TTC AAG TTC TTG AAG CCT ATA TTC CCA     3078 
Gly Gly Ser Glu Ser Asn Asp Phe Lys Phe Leu Lys Pro Ile Phe Pro 
            760                 765                 770 

AAT ATT GTA AGC CAG GAC TCT AGG AGG AAA TCA GAA TTT CTA GAA         3123 
Asn Ile Val Ser Gln Asp Ser Arg Arg Lys Ser Glu Phe Leu Glu 
        775                 780                 785 

TAATGTAGGT ATCTGTAGCT TTCCGACCGT CTGTTAATTT TGTCTTCCTC ACTTTTCACC   3183 

TTAGTTTTTT TTAACCCTTT AGTAATCTTG AATTCTACTT TTTTTTAAAT TTCTACTGTT   3243 

GTCTTTAGTA ATGTTACTCA TTTCCTTTGT CTGATTGTTA GTTTTCAAAT TATTGTATTA   3303 

TTATAAATAT TTTATATCAG GAAAGTTCAT ATTTCTGAAT AAATTAATAG              3353 

 
           
           
             
               787 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              110 

Met Glu Pro Ala Gly Glu Arg Phe Pro Glu Gln Arg Gln Val Leu Ile 
  1               5                  10                  15 

Leu Leu Leu Leu Leu Glu Val Thr Leu Ala Gly Trp Glu Pro Arg Arg 
             20                  25                  30 

Tyr Ser Val Met Glu Glu Thr Glu Arg Gly Ser Phe Val Ala Asn Leu 
         35                  40                  45 

Ala Asn Asp Leu Gly Leu Gly Val Gly Glu Leu Ala Glu Arg Gly Ala 
     50                  55                  60 

Arg Val Val Ser Glu Asp Asn Glu Gln Gly Leu Gln Leu Asp Leu Gln 
 65                  70                  75                  80 

Thr Gly Gln Leu Ile Leu Asn Glu Lys Leu Asp Arg Glu Lys Leu Cys 
                 85                  90                  95 

Gly Pro Thr Glu Pro Cys Ile Met His Phe Gln Val Leu Leu Lys Lys 
            100                 105                 110 

Pro Leu Glu Val Phe Arg Ala Glu Leu Leu Val Thr Asp Ile Asn Asp 
        115                 120                 125 

His Ser Pro Glu Phe Pro Glu Arg Glu Met Thr Leu Lys Ile Pro Glu 
    130                 135                 140 

Thr Ser Ser Leu Gly Thr Val Phe Pro Leu Lys Lys Ala Arg Asp Leu 
145                 150                 155                 160 

Asp Val Gly Ser Asn Asn Val Gln Asn Tyr Asn Ile Ser Pro Asn Ser 
                165                 170                 175 

His Phe His Val Ser Thr Arg Thr Arg Gly Asp Gly Arg Lys Tyr Pro 
            180                 185                 190 

Glu Leu Val Leu Asp Thr Glu Leu Asp Arg Glu Glu Gln Ala Glu Leu 
        195                 200                 205 

Arg Leu Thr Leu Thr Ala Val Asp Gly Gly Ser Pro Pro Arg Ser Gly 
    210                 215                 220 

Thr Val Gln Ile Leu Ile Leu Val Leu Asp Ala Asn Asp Asn Ala Pro 
225                 230                 235                 240 

Glu Phe Val Gln Ala Leu Tyr Glu Val Gln Val Pro Glu Asn Ser Pro 
                245                 250                 255 

Val Gly Ser Leu Val Val Lys Val Ser Ala Arg Asp Leu Asp Thr Gly 
            260                 265                 270 

Thr Asn Gly Glu Ile Ser Tyr Ser Leu Tyr Tyr Ser Ser Gln Glu Ile 
        275                 280                 285 

Asp Lys Pro Phe Glu Leu Ser Ser Leu Ser Gly Glu Ile Arg Leu Ile 
    290                 295                 300 

Lys Lys Leu Asp Phe Glu Thr Met Ser Ser Tyr Asp Leu Asp Ile Glu 
305                 310                 315                 320 

Ala Ser Asp Gly Gly Gly Leu Ser Gly Lys Cys Ser Val Ser Val Lys 
                325                 330                 335 

Val Leu Asp Val Asn Asp Asn Phe Pro Glu Leu Ser Ile Ser Ser Leu 
            340                 345                 350 

Thr Ser Pro Ile Pro Glu Asn Ser Pro Glu Thr Glu Val Ala Leu Phe 
        355                 360                 365 

Arg Ile Arg Asp Arg Asp Ser Gly Glu Asn Gly Lys Met Ile Cys Ser 
    370                 375                 380 

Ile Gln Asp Asp Val Pro Phe Lys Leu Lys Pro Ser Val Glu Asn Phe 
385                 390                 395                 400 

Tyr Arg Leu Val Thr Glu Gly Ala Leu Asp Arg Glu Thr Arg Ala Glu 
                405                 410                 415 

Tyr Asn Ile Thr Ile Thr Ile Thr Asp Leu Gly Thr Pro Arg Leu Lys 
            420                 425                 430 

Thr Glu Gln Ser Ile Thr Val Leu Val Ser Asp Val Asn Asp Asn Ala 
        435                 440                 445 

Pro Ala Phe Thr Gln Thr Ser Tyr Thr Leu Phe Val Arg Glu Asn Asn 
    450                 455                 460 

Ser Pro Ala Leu His Ile Gly Ser Val Ser Ala Thr Asp Arg Asp Ser 
465                 470                 475                 480 

Gly Thr Asn Ala Gln Val Thr Tyr Ser Leu Leu Pro Pro Gln Asp Pro 
                485                 490                 495 

His Leu Pro Leu Thr Ser Leu Val Ser Ile Asn Thr Asp Asn Gly His 
            500                 505                 510 

Leu Phe Ala Leu Gln Ser Leu Asp Tyr Glu Ala Leu Gln Ala Phe Glu 
        515                 520                 525 

Phe Arg Val Gly Ala Thr Asp Arg Gly Phe Pro Ala Leu Ser Ser Glu 
    530                 535                 540 

Ala Leu Val Arg Val Leu Val Leu Asp Ala Asn Asp Asn Ser Pro Phe 
545                 550                 555                 560 

Val Leu Tyr Pro Leu Gln Asn Gly Ser Ala Pro Cys Thr Glu Leu Val 
                565                 570                 575 

Pro Arg Ala Ala Glu Pro Gly Tyr Leu Val Thr Lys Val Val Ala Val 
            580                 585                 590 

Asp Gly Asp Ser Gly Gln Asn Ala Trp Leu Ser Tyr Gln Leu Leu Lys 
        595                 600                 605 

Ala Thr Glu Pro Gly Leu Phe Gly Val Trp Ala His Asn Gly Glu Val 
    610                 615                 620 

Arg Thr Ala Arg Leu Leu Ser Glu Arg Asp Val Ala Lys His Arg Leu 
625                 630                 635                 640 

Val Val Leu Val Lys Asp Asn Gly Glu Pro Pro Arg Ser Ala Thr Ala 
                645                 650                 655 

Thr Leu Gln Val Leu Leu Val Asp Gly Phe Ser Gln Pro Tyr Leu Pro 
            660                 665                 670 

Leu Pro Glu Ala Ala Pro Ala Gln Ala Gln Ala Asp Ser Leu Thr Val 
        675                 680                 685 

Tyr Leu Val Val Ala Leu Ala Ser Val Ser Ser Leu Phe Leu Phe Ser 
    690                 695                 700 

Val Phe Leu Phe Val Ala Val Arg Leu Cys Arg Arg Ser Arg Ala Ala 
705                 710                 715                 720 

Ser Val Gly Arg Cys Ser Val Pro Glu Gly Pro Phe Pro Gly His Leu 
                725                 730                 735 

Val Asp Val Ser Gly Thr Gly Thr Leu Ser Gln Ser Tyr Gln Tyr Glu 
            740                 745                 750 

Val Cys Leu Thr Gly Gly Ser Glu Ser Asn Asp Phe Lys Phe Leu Lys 
        755                 760                 765 

Pro Ile Phe Pro Asn Ile Val Ser Gln Asp Ser Arg Arg Lys Ser Glu 
    770                 775                 780 

Phe Leu Glu 
785 

 
           
           
             
               3033 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
             
               CDS  
                138..2528 

 
             
              111 

GTGATTGGAC GTGTTTTTGT GACTATTTGG GAAGAAGACA CCTTCCTAAT CAGATTTACT     60 

CCAATATCTT CCCGGACCCT CATGAGTGGA TTGCAATTGA CTTGAAGAAG CAGCACCCTC    120 

AGGACTGAAT CTGAACA ATG GAG ACA GCA CTA GCA AAA ATA CCA CAG CAA       170 
                   Met Glu Thr Ala Leu Ala Lys Ile Pro Gln Gln 
                     1               5                  10 

AGG CAA GTC TTT TTT CTT ACT ATA TTG TCG TTA TTG TGG AAG TCT AGC      218 
Arg Gln Val Phe Phe Leu Thr Ile Leu Ser Leu Leu Trp Lys Ser Ser 
             15                  20                  25 

TCT GAG GCC ATT AGA TAT TCC ATG CCA GAA GAA ACA GAG AGT GGC TAT      266 
Ser Glu Ala Ile Arg Tyr Ser Met Pro Glu Glu Thr Glu Ser Gly Tyr 
         30                  35                  40 

ATG GTG GCT AAC CTG GCG AAA GAT CTG GGG ATC AGG GTT GGA GAA CTG      314 
Met Val Ala Asn Leu Ala Lys Asp Leu Gly Ile Arg Val Gly Glu Leu 
     45                  50                  55 

TCC TCT AGA GGA GCT CAA ATC CAT TAC AAA GGA AAC AAA GAA CTT TTG      362 
Ser Ser Arg Gly Ala Gln Ile His Tyr Lys Gly Asn Lys Glu Leu Leu 
 60                  65                  70                  75 

CAG CTG GAT GCA GAG ACT GGG AAT TTG TTC TTA AAG GAA AAA CTA GAC      410 
Gln Leu Asp Ala Glu Thr Gly Asn Leu Phe Leu Lys Glu Lys Leu Asp 
                 80                  85                  90 

AGA GAA CTG CTG TGT GGA GAG ACA GAA CCC TGT GTG CTG AAC TTC CAG      458 
Arg Glu Leu Leu Cys Gly Glu Thr Glu Pro Cys Val Leu Asn Phe Gln 
             95                 100                 105 

ATC ATA CTG GAA AAC CCT ATG CAG TTC TTC CAA ACT GAA CTG CAG CTC      506 
Ile Ile Leu Glu Asn Pro Met Gln Phe Phe Gln Thr Glu Leu Gln Leu 
        110                 115                 120 

ACA GAT ATA AAC GAC CAT TCT CCA GAG TTC CCC AAC AAG AAA ATG CTT      554 
Thr Asp Ile Asn Asp His Ser Pro Glu Phe Pro Asn Lys Lys Met Leu 
    125                 130                 135 

CTA ACA ATT CCT GAG AGT GCC CAT CCA GGG ACT GTG TTT CCT CTG AAG      602 
Leu Thr Ile Pro Glu Ser Ala His Pro Gly Thr Val Phe Pro Leu Lys 
140                 145                 150                 155 

GCA GCT CGG GAC TCT GAC ATA GGG AGC AAC GCT GTT CAG AAC TAC ACA      650 
Ala Ala Arg Asp Ser Asp Ile Gly Ser Asn Ala Val Gln Asn Tyr Thr 
                160                 165                 170 

GTC AAT CCC AAC CTC CAT TTC CAC GTC GTT ACT CAC AGT CGC ACA GAT      698 
Val Asn Pro Asn Leu His Phe His Val Val Thr His Ser Arg Thr Asp 
            175                 180                 185 

GGC AGG AAA TAC CCA GAG CTG GTG CTG GAC AGA GCC CTG GAT AGG GAG      746 
Gly Arg Lys Tyr Pro Glu Leu Val Leu Asp Arg Ala Leu Asp Arg Glu 
        190                 195                 200 

GAG CAG CCT GAG CTC ACT TTA ATC CTC ACT GCT CTG GAT GGT GGA GCT      794 
Glu Gln Pro Glu Leu Thr Leu Ile Leu Thr Ala Leu Asp Gly Gly Ala 
    205                 210                 215 

CCT TCC AGG TCA GGA ACC ACC ACA GTT CAC ATA GAA GTT GTG GAC ATC      842 
Pro Ser Arg Ser Gly Thr Thr Thr Val His Ile Glu Val Val Asp Ile 
220                 225                 230                 235 

AAT GAT AAC TCC CCC CAG TTT GTA CAG TCA CTC TAT AAG GTG CAA GTT      890 
Asn Asp Asn Ser Pro Gln Phe Val Gln Ser Leu Tyr Lys Val Gln Val 
                240                 245                 250 

CCT GAG AAT AAT CCC CTC AAT GCC TTT GTT GTC ACG GTC TCT GCC ACG      938 
Pro Glu Asn Asn Pro Leu Asn Ala Phe Val Val Thr Val Ser Ala Thr 
            255                 260                 265 

GAT TTA GAT GCT GGG GTA TAT GGC AAT GTG ACC TAT TCT CTG TTT CAA      986 
Asp Leu Asp Ala Gly Val Tyr Gly Asn Val Thr Tyr Ser Leu Phe Gln 
        270                 275                 280 

GGG TAT GGG GTA TTT CAA CCA TTT GTA ATA GAC GAA ATC ACT GGA GAA     1034 
Gly Tyr Gly Val Phe Gln Pro Phe Val Ile Asp Glu Ile Thr Gly Glu 
    285                 290                 295 

ATC CAT CTG AGC AAA GAG CTG GAT TTT GAG GAA ATT AGC AAT CAT AAC     1082 
Ile His Leu Ser Lys Glu Leu Asp Phe Glu Glu Ile Ser Asn His Asn 
300                 305                 310                 315 

ATA GAA ATC GCA GCC ACA GAT GGA GGA GGC CTT TCA GGA AAA TGC ACT     1130 
Ile Glu Ile Ala Ala Thr Asp Gly Gly Gly Leu Ser Gly Lys Cys Thr 
                320                 325                 330 

GTG GCT GTA CAG GTG TTG GAT GTG AAT GAC AAC GCC CCA GAG TTG ACA     1178 
Val Ala Val Gln Val Leu Asp Val Asn Asp Asn Ala Pro Glu Leu Thr 
            335                 340                 345 

ATT AGG AAG CTC ACA GTC CTG GTC CCA GAA AAT TCC GCA GAG ACT GTA     1226 
Ile Arg Lys Leu Thr Val Leu Val Pro Glu Asn Ser Ala Glu Thr Val 
        350                 355                 360 

GTT GCT GTT TTT AGT GTT TCT GAT TCT GAT TCG GGG GAC AAT GGA AGG     1274 
Val Ala Val Phe Ser Val Ser Asp Ser Asp Ser Gly Asp Asn Gly Arg 
    365                 370                 375 

ATG GTG TGT TCT ATT CCG AAC AAT ATC CCA TTT CTC CTG AAA CCC ACA     1322 
Met Val Cys Ser Ile Pro Asn Asn Ile Pro Phe Leu Leu Lys Pro Thr 
380                 385                 390                 395 

TTT GAG AAT TAT TAC ACG TTA GTG ACT GAG GGG CCA CTT GAT AGA GAG     1370 
Phe Glu Asn Tyr Tyr Thr Leu Val Thr Glu Gly Pro Leu Asp Arg Glu 
                400                 405                 410 

AAC AGA GCT GAG TAC AAC ATC ACC ATC ACG GTC TCA GAT CTG GGC ACA     1418 
Asn Arg Ala Glu Tyr Asn Ile Thr Ile Thr Val Ser Asp Leu Gly Thr 
            415                 420                 425 

CCC AGG CTC ACA ACC CAG CAC ACC ATA ACA GTG CAA GTG TCC GAC ATC     1466 
Pro Arg Leu Thr Thr Gln His Thr Ile Thr Val Gln Val Ser Asp Ile 
        430                 435                 440 

AAC GAC AAC GCC CCT GCC TTC ACC CAA ACC TCC TAC ACC ATG TTT GTC     1514 
Asn Asp Asn Ala Pro Ala Phe Thr Gln Thr Ser Tyr Thr Met Phe Val 
    445                 450                 455 

CAC GAG AAC AAC AGC CCC GCC CTG CAC ATA GGC ACC ATC AGT GCC ACA     1562 
His Glu Asn Asn Ser Pro Ala Leu His Ile Gly Thr Ile Ser Ala Thr 
460                 465                 470                 475 

GAC TCA GAC TCA GGC TCC AAT GCC CAC ATC ACC TAC TCG CTG CTG CCG     1610 
Asp Ser Asp Ser Gly Ser Asn Ala His Ile Thr Tyr Ser Leu Leu Pro 
                480                 485                 490 

CCT GAT GAC CCG CAG CTG GCC CTC GAC TCA CTC ATC TCC ATC AAT GTT     1658 
Pro Asp Asp Pro Gln Leu Ala Leu Asp Ser Leu Ile Ser Ile Asn Val 
            495                 500                 505 

GAC AAT GGG CAG CTG TTC GCG CTC AGA GCT CTA GAC TAT GAG GCA CTG     1706 
Asp Asn Gly Gln Leu Phe Ala Leu Arg Ala Leu Asp Tyr Glu Ala Leu 
        510                 515                 520 

CAG TCC TTC GAG TTC TAC GTG GGC GCT ACA GAT GGA GGC TCA CCC GCG     1754 
Gln Ser Phe Glu Phe Tyr Val Gly Ala Thr Asp Gly Gly Ser Pro Ala 
    525                 530                 535 

CTC AGC AGC CAG ACT CTG GTG CGG ATG GTG GTG CTG GAT GAC AAT GAC     1802 
Leu Ser Ser Gln Thr Leu Val Arg Met Val Val Leu Asp Asp Asn Asp 
540                 545                 550                 555 

AAT GCC CCC TTC GTG CTC TAC CCA CTG CAG AAT GCC TCA GCA CCC TGT     1850 
Asn Ala Pro Phe Val Leu Tyr Pro Leu Gln Asn Ala Ser Ala Pro Cys 
                560                 565                 570 

ACT GAG CTA CTG CCT AGG GCA GCA GAG CCC GGC TAC CTG ATC ACC AAA     1898 
Thr Glu Leu Leu Pro Arg Ala Ala Glu Pro Gly Tyr Leu Ile Thr Lys 
            575                 580                 585 

GTG GTG GCT GTG GAT CGC GAC TCT GGA CAG AAT GCT TGG CTG TCG TTC     1946 
Val Val Ala Val Asp Arg Asp Ser Gly Gln Asn Ala Trp Leu Ser Phe 
        590                 595                 600 

CAG CTA CTT AAA GCT ACA GAG CCA GGG CTG TTC AGT GTA TGG GCA CAC     1994 
Gln Leu Leu Lys Ala Thr Glu Pro Gly Leu Phe Ser Val Trp Ala His 
    605                 610                 615 

AAT GGT GAA GTG CGC ACC ACT AGG CTG CTG AGT GAG CGA GAT GCT CAG     2042 
Asn Gly Glu Val Arg Thr Thr Arg Leu Leu Ser Glu Arg Asp Ala Gln 
620                 625                 630                 635 

AAG CAC AAG CTA CTG CTG CTG GTC AAG GAC AAT GGC GAT CCT CTG CGC     2090 
Lys His Lys Leu Leu Leu Leu Val Lys Asp Asn Gly Asp Pro Leu Arg 
                640                 645                 650 

TCT GCC AAT GTC ACT CTT CAC GTG CTA GTG GTG GAT GGC TTC TCG CAG     2138 
Ser Ala Asn Val Thr Leu His Val Leu Val Val Asp Gly Phe Ser Gln 
            655                 660                 665 

CCT TAC CTA CCA TTG GCT GAG GTG GCA CAG GAT TCC ATG CAA GAT AAT     2186 
Pro Tyr Leu Pro Leu Ala Glu Val Ala Gln Asp Ser Met Gln Asp Asn 
        670                 675                 680 

TAC GAC GTT CTC ACA CTG TAC CTA GTC ATT GCC TTG GCA TCT GTA TCT     2234 
Tyr Asp Val Leu Thr Leu Tyr Leu Val Ile Ala Leu Ala Ser Val Ser 
    685                 690                 695 

TCT CTC TTC CTC TTG TCT GTA GTG CTG TTT GTG GGG GTG AGG CTG TGC     2282 
Ser Leu Phe Leu Leu Ser Val Val Leu Phe Val Gly Val Arg Leu Cys 
700                 705                 710                 715 

AGG AGG GCC AGG GAG GCC TCC TTG GGT GAC TAC TCT GTG CCT GAG GGA     2330 
Arg Arg Ala Arg Glu Ala Ser Leu Gly Asp Tyr Ser Val Pro Glu Gly 
                720                 725                 730 

CAC TTT CCT AGC CAC TTG GTG GAT GTC AGC GGT GCC GGG ACC CTG TCC     2378 
His Phe Pro Ser His Leu Val Asp Val Ser Gly Ala Gly Thr Leu Ser 
            735                 740                 745 

CAG AGT TAT CAA TAT GAG GTG TGT CTT AAT GGA GGT ACT AGA ACA AAT     2426 
Gln Ser Tyr Gln Tyr Glu Val Cys Leu Asn Gly Gly Thr Arg Thr Asn 
        750                 755                 760 

GAG TTT AAC TTT CTT AAA CCA TTG TTT CCT ATC CTT CCG ACC CAG GCT     2474 
Glu Phe Asn Phe Leu Lys Pro Leu Phe Pro Ile Leu Pro Thr Gln Ala 
    765                 770                 775 

GCT GCT GCT GAA GAA AGA GAA AAC GCT GTT GTG CAC AAT AGC GTT GGA     2522 
Ala Ala Ala Glu Glu Arg Glu Asn Ala Val Val His Asn Ser Val Gly 
780                 785                 790                 795 

TTC TAT TAGAGCACTG ATTTTGAAGT GGTGGTTACC TCATTTTTCC TTAACTATCC      2578 
Phe Tyr 

CTGATGTAGA ATGGTGTAGT GCCGTGAATC AACTCCTGAG ATATATGTTC ATTTTATCCT   2638 

TTGTTTTGAA TCAAACTATT CAGATGTGAT CCTACTCTAG AGAATTTGGT TCTACTCCAT   2698 

TGTGTTTGTT TAGATTTCTA CGCCATACCA GTGCATGCTG GGTTGTTTTT TTTTTTACAA   2758 

TTATTATAAC TTTGCTTTGG AGGGGAACTC ATATTCGCTG TAACGAATTG GAACCACTTT   2818 

CATTGTTAGA GATGCCTTGC TTTGTTGTGT TATTTCAGAC AGGGTCTTAA ATTGTAGCCC   2878 

TGGGTGACCT GAAATGACTA TGTACAGACT GACTTTGAAT TTGTGGCAGT CCATCTGCCT   2938 

CTGTTGTCCT ATGTTGGGAT TGTGAGCATG CATGAGTAGG CTCAGCTGTG GTGAGCGACC   2998 

TTAATAAAAA TCAAATACTA AAAAAAAAAA AAAAA                              3033 

 
           
           
             
               797 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              112 

Met Glu Thr Ala Leu Ala Lys Ile Pro Gln Gln Arg Gln Val Phe Phe 
  1               5                  10                  15 

Leu Thr Ile Leu Ser Leu Leu Trp Lys Ser Ser Ser Glu Ala Ile Arg 
             20                  25                  30 

Tyr Ser Met Pro Glu Glu Thr Glu Ser Gly Tyr Met Val Ala Asn Leu 
         35                  40                  45 

Ala Lys Asp Leu Gly Ile Arg Val Gly Glu Leu Ser Ser Arg Gly Ala 
     50                  55                  60 

Gln Ile His Tyr Lys Gly Asn Lys Glu Leu Leu Gln Leu Asp Ala Glu 
 65                  70                  75                  80 

Thr Gly Asn Leu Phe Leu Lys Glu Lys Leu Asp Arg Glu Leu Leu Cys 
                 85                  90                  95 

Gly Glu Thr Glu Pro Cys Val Leu Asn Phe Gln Ile Ile Leu Glu Asn 
            100                 105                 110 

Pro Met Gln Phe Phe Gln Thr Glu Leu Gln Leu Thr Asp Ile Asn Asp 
        115                 120                 125 

His Ser Pro Glu Phe Pro Asn Lys Lys Met Leu Leu Thr Ile Pro Glu 
    130                 135                 140 

Ser Ala His Pro Gly Thr Val Phe Pro Leu Lys Ala Ala Arg Asp Ser 
145                 150                 155                 160 

Asp Ile Gly Ser Asn Ala Val Gln Asn Tyr Thr Val Asn Pro Asn Leu 
                165                 170                 175 

His Phe His Val Val Thr His Ser Arg Thr Asp Gly Arg Lys Tyr Pro 
            180                 185                 190 

Glu Leu Val Leu Asp Arg Ala Leu Asp Arg Glu Glu Gln Pro Glu Leu 
        195                 200                 205 

Thr Leu Ile Leu Thr Ala Leu Asp Gly Gly Ala Pro Ser Arg Ser Gly 
    210                 215                 220 

Thr Thr Thr Val His Ile Glu Val Val Asp Ile Asn Asp Asn Ser Pro 
225                 230                 235                 240 

Gln Phe Val Gln Ser Leu Tyr Lys Val Gln Val Pro Glu Asn Asn Pro 
                245                 250                 255 

Leu Asn Ala Phe Val Val Thr Val Ser Ala Thr Asp Leu Asp Ala Gly 
            260                 265                 270 

Val Tyr Gly Asn Val Thr Tyr Ser Leu Phe Gln Gly Tyr Gly Val Phe 
        275                 280                 285 

Gln Pro Phe Val Ile Asp Glu Ile Thr Gly Glu Ile His Leu Ser Lys 
    290                 295                 300 

Glu Leu Asp Phe Glu Glu Ile Ser Asn His Asn Ile Glu Ile Ala Ala 
305                 310                 315                 320 

Thr Asp Gly Gly Gly Leu Ser Gly Lys Cys Thr Val Ala Val Gln Val 
                325                 330                 335 

Leu Asp Val Asn Asp Asn Ala Pro Glu Leu Thr Ile Arg Lys Leu Thr 
            340                 345                 350 

Val Leu Val Pro Glu Asn Ser Ala Glu Thr Val Val Ala Val Phe Ser 
        355                 360                 365 

Val Ser Asp Ser Asp Ser Gly Asp Asn Gly Arg Met Val Cys Ser Ile 
    370                 375                 380 

Pro Asn Asn Ile Pro Phe Leu Leu Lys Pro Thr Phe Glu Asn Tyr Tyr 
385                 390                 395                 400 

Thr Leu Val Thr Glu Gly Pro Leu Asp Arg Glu Asn Arg Ala Glu Tyr 
                405                 410                 415 

Asn Ile Thr Ile Thr Val Ser Asp Leu Gly Thr Pro Arg Leu Thr Thr 
            420                 425                 430 

Gln His Thr Ile Thr Val Gln Val Ser Asp Ile Asn Asp Asn Ala Pro 
        435                 440                 445 

Ala Phe Thr Gln Thr Ser Tyr Thr Met Phe Val His Glu Asn Asn Ser 
    450                 455                 460 

Pro Ala Leu His Ile Gly Thr Ile Ser Ala Thr Asp Ser Asp Ser Gly 
465                 470                 475                 480 

Ser Asn Ala His Ile Thr Tyr Ser Leu Leu Pro Pro Asp Asp Pro Gln 
                485                 490                 495 

Leu Ala Leu Asp Ser Leu Ile Ser Ile Asn Val Asp Asn Gly Gln Leu 
            500                 505                 510 

Phe Ala Leu Arg Ala Leu Asp Tyr Glu Ala Leu Gln Ser Phe Glu Phe 
        515                 520                 525 

Tyr Val Gly Ala Thr Asp Gly Gly Ser Pro Ala Leu Ser Ser Gln Thr 
    530                 535                 540 

Leu Val Arg Met Val Val Leu Asp Asp Asn Asp Asn Ala Pro Phe Val 
545                 550                 555                 560 

Leu Tyr Pro Leu Gln Asn Ala Ser Ala Pro Cys Thr Glu Leu Leu Pro 
                565                 570                 575 

Arg Ala Ala Glu Pro Gly Tyr Leu Ile Thr Lys Val Val Ala Val Asp 
            580                 585                 590 

Arg Asp Ser Gly Gln Asn Ala Trp Leu Ser Phe Gln Leu Leu Lys Ala 
        595                 600                 605 

Thr Glu Pro Gly Leu Phe Ser Val Trp Ala His Asn Gly Glu Val Arg 
    610                 615                 620 

Thr Thr Arg Leu Leu Ser Glu Arg Asp Ala Gln Lys His Lys Leu Leu 
625                 630                 635                 640 

Leu Leu Val Lys Asp Asn Gly Asp Pro Leu Arg Ser Ala Asn Val Thr 
                645                 650                 655 

Leu His Val Leu Val Val Asp Gly Phe Ser Gln Pro Tyr Leu Pro Leu 
            660                 665                 670 

Ala Glu Val Ala Gln Asp Ser Met Gln Asp Asn Tyr Asp Val Leu Thr 
        675                 680                 685 

Leu Tyr Leu Val Ile Ala Leu Ala Ser Val Ser Ser Leu Phe Leu Leu 
    690                 695                 700 

Ser Val Val Leu Phe Val Gly Val Arg Leu Cys Arg Arg Ala Arg Glu 
705                 710                 715                 720 

Ala Ser Leu Gly Asp Tyr Ser Val Pro Glu Gly His Phe Pro Ser His 
                725                 730                 735 

Leu Val Asp Val Ser Gly Ala Gly Thr Leu Ser Gln Ser Tyr Gln Tyr 
            740                 745                 750 

Glu Val Cys Leu Asn Gly Gly Thr Arg Thr Asn Glu Phe Asn Phe Leu 
        755                 760                 765 

Lys Pro Leu Phe Pro Ile Leu Pro Thr Gln Ala Ala Ala Ala Glu Glu 
    770                 775                 780 

Arg Glu Asn Ala Val Val His Asn Ser Val Gly Phe Tyr 
785                 790                 795 

 
           
           
             
               2347 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
              113 

AAAACACGGG GGAAATGACA GTAGCAAAGA ATCTGGACTA TGAAGAATGC TCATTGTATG     60 

AAATGGAAAT ACAGGCTGAA GATGTGGGGG CGCTTCTGGG GAGGAGCAAA GTGGTAATTA    120 

TGGTAGAAGA TGTAAATGAC AATCGGCCAG AAGTGACCAT TACATCCTTG TTTAACCCGG    180 

TATTGGAAAA TTCTCTTCCC GGGACAGTAA TTGCCTTCTT GAATGTGCAT GACCGAGACT    240 

CTGGAAAGAA CGGCCAAGTT GTCTGTTACA CGCATGATAA CTTACCTTTT AAATTAGAAA    300 

AGTCAATAGA TAATTATTAT AGATTGGTGA CATGGAAATA TTTGGACCGA GAAAAAGTCT    360 

CCATCTACAA TATCACAGTG ATAGCCTCAG ATCTAGGAGC CCACTCTGTC ACTGAAACTT    420 

ACATTGCCCT GATTGTGGCA GACACTAATG ACAACCCTCC TCGTTTTCCT CACACCTCCT    480 

ACACAGCCTA TATTCCAGAG AACAACCTGA GGGGCGCCTC CATCTTCTCA CTGACTGCAC    540 

ATGATCCTGA CAGTCAGGAA AATGCACAGG TCACTTACTC TGTGTCTGAG GACACCATAC    600 

AGGGAGTGCC TTTGTCCTCT TATATCTCCA TCAACTCAGA TACTGGTGTC CTGTATGCAC    660 

TGCACTCTTT TGACTTCGAG AAGATACAAG ACTTGCAGCT ACTGGTTGTT GCCACTGACA    720 

GTGGAAGCCC ACCTCTCAGC AGCAATGTGT CATTGAGCTT GTTTGTGTTG GACCAGAACG    780 

ACAACGCACC TGAGATTCTA TATCCTAGCT TCCCCACAGA TGGCTCCACT GGTGTGGAAC    840 

TAGCACCCCG CTCTGCAGAG CCTGGATACC TAGTGACCAA AGTGGTGGCA GTGGACAAAG    900 

ACTCAGGACA GAATGCTTGG CTGTCCTACC GTCTGCTGAA GGCCAGCGAA CCTGGGCTCT    960 

TCTCTGTAGG ACTTCACACG GGTGAGGTGC GTACAGCGAG GGCCCTGCTG GACAGAGATG   1020 

CTCTCAAACA GAATCTGGTG ATGGCCGTGC AGGACCATGG CCAACCCCCT CTCTCGGCCA   1080 

CTGTAACTCT CACTGTGGCA GTGGCTAACA GCATCCCTGA GGTGTTGGCT GACTTGAGCA   1140 

GCATTAGGAC CCCTGGGGTA CCAGAGGATT CTGATATCAC GCTCCACCTG GTGGTGGCAG   1200 

TGGCTGTGGT CTCCTGTGTC TTCCTTGTCT TTGTCATTGT CCTCCTAGCT CTCAGGCTTC   1260 

AGCGCTGGCA GAAGTCTCGC CAGCTCCAGG GCTCCAAAGG TGGATTGGCT CCTGCACCTC   1320 

CATCACATTT TGTGGGCATC GACGGGGTAC AGGCTTTTCT ACAAACCTAT TCTCATGAAG   1380 

TCTCGCTCAC TTCAGGCTCC CAGACAAGCC ACATTATCTT TCCTCAGCCC AACTATGCAG   1440 

ACATGCTCAT TAACCAAGAA GGCTGTGAGA AAAATGATTC CTTATTAACA TCCATAGATT   1500 

TTCATGAGAG TAACCGTGAA GATGCTTGCG CCCCGCAAGC CCCGCCCAAC ACTGACTGGC   1560 

GTTTCTCTCA AGCCCAGAGA CCCGGCACGA GCGGATCCCA AAATGGGGAT GAAACCGGCA   1620 

CCTGGCCCAA CAACCAGTTC GATACAGAGA TGCTGCAAGC CATGATCTTG GCCTCTGCCA   1680 

GTGAAGCCGC TGATGGGAGC TCCACTCTGG GAGGGGGCAC TGGCACTATG GGTTTGAGCG   1740 

CTCGATATGG ACCCCAGTTT ACCCTGCAGC ACGTGCCTGA CTACCGCCAG AACGTGTACA   1800 

TCCCTGGCAG CAATGCCACA CTGACCAACG CAGCTGGCAA ACGAGATGGC AAGGCTCCGG   1860 

CAGGCGGCAA TGGCAACAAC AACAAGTCGG GCAAGAAAGA GAAGAAGTAA TATGGAGGCC   1920 

AGGCCTTGAG CCACAGGGCA GCCTCCCTCC CCAGCCAGTC CAGCTTGTCC TTACTTGTAC   1980 

CCAGGCCTCA GAATTTCAGG GCTCACCCCA GGATTCTGGT AGGAGCCACA GCCAGGCCAT   2040 

GCTCCCCGTT GGGAAACAGA AACAAGTGCC CAAGCCAACA CCCCCTCTTT GTACCCTAGG   2100 

GGGGTTGAAT ATGCAAAGAG AGTTCTGCTG GGACCCCCTA TCCAATCAGT GATTGTACCC   2160 

ACATAGGTAG CAGGGTTAGT GTGGATACAC ACACACACAC ACACACACAC ACACACACAA   2220 

CCCTTGTCCT CCGCAGTGCC TGCCACTTTC TGGGACTTTC TCATCCCCCT ACGCCCTTCC   2280 

TTTATCCTCT CCCACCCAGA CACAGCTGCT GGAGAATAAA TTTGGGGATG CTGATGCTAA   2340 

AAAAAAA                                                             2347 

 
           
           
             
               2972 base pairs  
               nucleic acid  
               single  
               linear  
             
             
               cDNA  
             
             
               CDS  
                2..1849 

 
             
              114 

A GAG GCT GCT CAC CAC CTG GTC CTC ACG GCC TCG GAT GGC GGC AAG         46 
  Glu Ala Ala His His Leu Val Leu Thr Ala Ser Asp Gly Gly Lys 
    1               5                  10                  15 

CCG CCT CGC TCT AGC ACA GTG CGC ATC CAC GTG ACA GTG TTG GAT ACA       94 
Pro Pro Arg Ser Ser Thr Val Arg Ile His Val Thr Val Leu Asp Thr 
                 20                  25                  30 

AAT GAC AAT GCC CCG GTT TTT CCT CAC CCG ATT TAC CGA GTG AAA GTC      142 
Asn Asp Asn Ala Pro Val Phe Pro His Pro Ile Tyr Arg Val Lys Val 
             35                  40                  45 

CTT GAG AAC ATG CCC CCA GGC ACG CGG CTG CTT ACT GTA ACA GCC AGC      190 
Leu Glu Asn Met Pro Pro Gly Thr Arg Leu Leu Thr Val Thr Ala Ser 
         50                  55                  60 

GAC CCG GAT GAG GGA ATC AAC GGA AAA GTG GCA TAC AAA TTC CGG AAA      238 
Asp Pro Asp Glu Gly Ile Asn Gly Lys Val Ala Tyr Lys Phe Arg Lys 
     65                  70                  75 

ATT AAT GAA AAA CAA ACT CCG TTA TTC CAG CTT AAT GAA AAT ACT GGG      286 
Ile Asn Glu Lys Gln Thr Pro Leu Phe Gln Leu Asn Glu Asn Thr Gly 
 80                  85                  90                  95 

GAA ATA TCA ATA GCA AAA AGT CTA GAT TAT GAA GAA TGT TCA TTT TAT      334 
Glu Ile Ser Ile Ala Lys Ser Leu Asp Tyr Glu Glu Cys Ser Phe Tyr 
                100                 105                 110 

GAA ATG GAA ATA CAA GCC GAA GAT GTG GGG GCA CTT CTG GGG AGG ACC      382 
Glu Met Glu Ile Gln Ala Glu Asp Val Gly Ala Leu Leu Gly Arg Thr 
            115                 120                 125 

AAA TTG CTC ATT TCT GTG GAA GAT GTA AAT GAC AAT AGA CCA GAA GTG      430 
Lys Leu Leu Ile Ser Val Glu Asp Val Asn Asp Asn Arg Pro Glu Val 
        130                 135                 140 

ATC ATT ACG TCT TTG TTT AGC CCA GTG TTA GAA AAT TCT CTT CCC GGG      478 
Ile Ile Thr Ser Leu Phe Ser Pro Val Leu Glu Asn Ser Leu Pro Gly 
    145                 150                 155 

ACA GTA ATT GCC TTC TTG AGT GTG CAT GAC CAA GAC TCT GGA AAG AAT      526 
Thr Val Ile Ala Phe Leu Ser Val His Asp Gln Asp Ser Gly Lys Asn 
160                 165                 170                 175 

GGT CAA GTT GTC TGT TAC ACA CGT GAT AAT TTA CCT TTT AAA TTA GAA      574 
Gly Gln Val Val Cys Tyr Thr Arg Asp Asn Leu Pro Phe Lys Leu Glu 
                180                 185                 190 

AAG TCA ATA GGT AAT TAT TAT AGA TTA GTG ACA AGG AAA TAT TTG GAC      622 
Lys Ser Ile Gly Asn Tyr Tyr Arg Leu Val Thr Arg Lys Tyr Leu Asp 
            195                 200                 205 

CGA GAA AAT GTC TCT ATC TAC AAT ATC ACA GTG ATG GCC TCA GAT CTA      670 
Arg Glu Asn Val Ser Ile Tyr Asn Ile Thr Val Met Ala Ser Asp Leu 
        210                 215                 220 

GGA ACA CCA CCT CTG TCC ACT GAA ACT CAA ATC GCT CTG CAC GTG GCA      718 
Gly Thr Pro Pro Leu Ser Thr Glu Thr Gln Ile Ala Leu His Val Ala 
    225                 230                 235 

GAC ATT AAC GAC AAC CCT CCT ACT TTC CCT CAT GCC TCC TAC TCA GCG      766 
Asp Ile Asn Asp Asn Pro Pro Thr Phe Pro His Ala Ser Tyr Ser Ala 
240                 245                 250                 255 

TAT ATC CTA GAG AAC AAC CTG AGA GGA GCC TCC ATC TTT TCC TTG ACT      814 
Tyr Ile Leu Glu Asn Asn Leu Arg Gly Ala Ser Ile Phe Ser Leu Thr 
                260                 265                 270 

GCA CAC GAC CCC GAC AGC CAG GAG AAT GCC CAG GTC ACT TAC TCT GTG      862 
Ala His Asp Pro Asp Ser Gln Glu Asn Ala Gln Val Thr Tyr Ser Val 
            275                 280                 285 

ACC GAG GAC ACG CTG CAG GGG GCG CCC CTG TCC TCG TAT ATC TCC ATC      910 
Thr Glu Asp Thr Leu Gln Gly Ala Pro Leu Ser Ser Tyr Ile Ser Ile 
        290                 295                 300 

AAC TCT GAC ACC GGT GTC CTG TAT GCG CTG CAA TCT TTC GAC TAT GAG      958 
Asn Ser Asp Thr Gly Val Leu Tyr Ala Leu Gln Ser Phe Asp Tyr Glu 
    305                 310                 315 

CAG ATC CGA GAC CTG CAG CTA CTG GTA ACA GCC AGC GAC AGC GGG GAC     1006 
Gln Ile Arg Asp Leu Gln Leu Leu Val Thr Ala Ser Asp Ser Gly Asp 
320                 325                 330                 335 

CCG CCC CTC AGC AGC AAC ATG TCA CTG AGC CTG TTC GTG CTG GAC CAG     1054 
Pro Pro Leu Ser Ser Asn Met Ser Leu Ser Leu Phe Val Leu Asp Gln 
                340                 345                 350 

AAT GAC AAC GCG CCC GAG ATC CTG TAC CCC GCC CTC CCC ACA GAC GGT     1102 
Asn Asp Asn Ala Pro Glu Ile Leu Tyr Pro Ala Leu Pro Thr Asp Gly 
            355                 360                 365 

TCC ACT GGC GTG GAG CTG GCG CCC CGC TCC GCA GAG CGT GGC TAC CTG     1150 
Ser Thr Gly Val Glu Leu Ala Pro Arg Ser Ala Glu Arg Gly Tyr Leu 
        370                 375                 380 

GTG ACC AAG GTG GTG GCG GTG GAC AGA GAC TCG GGC CAG AAC GCC TGG     1198 
Val Thr Lys Val Val Ala Val Asp Arg Asp Ser Gly Gln Asn Ala Trp 
    385                 390                 395 

CTG TCC TAC CGC CTG CTC AAG GCC AGC GAG CCG GGA CTC TTC TCG GTG     1246 
Leu Ser Tyr Arg Leu Leu Lys Ala Ser Glu Pro Gly Leu Phe Ser Val 
400                 405                 410                 415 

GGT CTG CAC ACG GGC GAG GTG CGC ACG GCG CGA GCC CTG CTG GAC AGA     1294 
Gly Leu His Thr Gly Glu Val Arg Thr Ala Arg Ala Leu Leu Asp Arg 
                420                 425                 430 

GAC GCG CTC AAG CAG AGC CTC GTG GTG GCC GTC CAG GAC CAT GGC CAG     1342 
Asp Ala Leu Lys Gln Ser Leu Val Val Ala Val Gln Asp His Gly Gln 
            435                 440                 445 

CCC CCT CTC TCC GCC ACT GTC ACG CTC ACC GTA GCC GTG GCT GAC AGC     1390 
Pro Pro Leu Ser Ala Thr Val Thr Leu Thr Val Ala Val Ala Asp Ser 
        450                 455                 460 

ATC CCC GAA GTC CTG ACC GAG TTG GGC AGT CTG AAG CCT TCG GTC GAC     1438 
Ile Pro Glu Val Leu Thr Glu Leu Gly Ser Leu Lys Pro Ser Val Asp 
    465                 470                 475 

CCG AAC GAT TCG AGC CTT ACA CTC TAT CTC GTG GTG GCA GTG GCT GCC     1486 
Pro Asn Asp Ser Ser Leu Thr Leu Tyr Leu Val Val Ala Val Ala Ala 
480                 485                 490                 495 

ATC TCC TGT GTC TTC CTC GCC TTT GTC GCT GTG CTT CTG GGG CTC AGG     1534 
Ile Ser Cys Val Phe Leu Ala Phe Val Ala Val Leu Leu Gly Leu Arg 
                500                 505                 510 

CTG AGG CGC TGG CAC AAG TCA CGC CTG CTC CAG GAT TCC GGT GGC AGA     1582 
Leu Arg Arg Trp His Lys Ser Arg Leu Leu Gln Asp Ser Gly Gly Arg 
            515                 520                 525 

TTG GTA GGC GTG CCT GCC TCA CAT TTT GTG GGT GTT GAG GAG GTA CAG     1630 
Leu Val Gly Val Pro Ala Ser His Phe Val Gly Val Glu Glu Val Gln 
        530                 535                 540 

GCT TTC CTG CAG ACC TAT TCC CAG GAA GTC TCC CTC ACC GCC GAC TCG     1678 
Ala Phe Leu Gln Thr Tyr Ser Gln Glu Val Ser Leu Thr Ala Asp Ser 
    545                 550                 555 

CGG AAG AGT CAC CTG ATC TTT CCC CAG CCC AAC TAC GCA GAC ATG CTC     1726 
Arg Lys Ser His Leu Ile Phe Pro Gln Pro Asn Tyr Ala Asp Met Leu 
560                 565                 570                 575 

ATC AGT CAG GAG GGC TGT GAG AAA AAT GAT TCT TTG TTA ACA TCC GTA     1774 
Ile Ser Gln Glu Gly Cys Glu Lys Asn Asp Ser Leu Leu Thr Ser Val 
                580                 585                 590 

GAT TTT CAT GAA TAT AAG AAT GAA GCT GAT CAT GGT CAG GTG AGT TTA     1822 
Asp Phe His Glu Tyr Lys Asn Glu Ala Asp His Gly Gln Val Ser Leu 
            595                 600                 605 

GTT CTT TGC TTG CTT TTA ATT TCC AGA TGAATTTTAT TTGGCATAAA           1869 
Val Leu Cys Leu Leu Leu Ile Ser Arg 
        610                 615 

TTATGTTTTG AAAAACATTG TGAAGATAGT TGAAAATAAT TTTTAAGGTG TATCACAGAG   1929 

TTTTGGGTTT ATTTTGGTGG TGTTACCAAA AAATTGAACT CTAATAGTCA TAGGTTATTG   1989 

TTTCATTTGC TTTTAAACGA CTTGGAAAAG ATTGTTCCAC CATTTTAAAC CTTCCAGTAT   2049 

TTTATTCCTA TTATCACTCA TTCACTTAAG AAGTAGCTAC CCGTCCATAC TGGTAATTTT   2109 

GCTATTGTTT GTTTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTAT CCCAAACTAG   2169 

AACTTCAGAA AATTATCAAG AAGTCTAAAG CCTTGTTATT AGCTTAGCAA AAGTAAAATA   2229 

TATCTCAGAA TTTTTAGGGT TATGTTTAGC ATTTGAACCT GTAACTAGGC TCTTGTATAT   2289 

TTCTTCACTT TAAACCTCTT TTCTGAGCCC TGTTTCTGTA CCAGTGCCCT TCAAAACTTT   2349 

AATACTTCTT ACCATCCTTC AAAACATGAA CAAACTTTAA AGATGGATCT TGGTGGGAGA   2409 

TGAGACTGGT TACTAAATAT TAAGTATGTG AGTCAGTGGT CACCTGGGCT CCATCCCCAT   2469 

GGAGACATGA AATCTAAAGC CTAGAATGTC CATTGCTCCC CCAAACAAAA AACAAAAGCA   2529 

AAAACATTAG ATCTGAATTA AAATGTAATT TTAAACTGTT GAAAGTGACT TTTGTAAAAT   2589 

ATGTAAGAAC ATATTTCAAT ACAATTCCAA TTAGCTGTTT CGGTTGTGCA TTGATGTGAA   2649 

GTGGTGAGAA TGTTGATATT AAGAACCAAT GTTTCAGGTA CACAAGTTCT AAATAAGCTG   2709 

ATCAATTCAA TTAAAGTTAT TCAGTCTTGG CTGGACACAG TGCCTCATGT CTGAAATCCC   2769 

AGCACTTTGG GAGGCTGGGG CAGGAGGACC GCTTGAGCCC CGGGGGTTTG AAACTGCAGT   2829 

GAGCTATGAT CATGCCACTG CACTCCAGCC TAGGTGGCAG AACTAGACCC TGTCTCTAAA   2889 

AAAACTATTA TTAGGCCGCG TGCGGTGGCT CACGCCTGTA ATCCCAGCAC TTTGGGAGAC   2949 

TGAGGTGGGT GGATCACCTG AGC                                           2972 

 
           
           
             
               616 amino acids  
               amino acid  
               linear  
             
             
               protein  
             
              115 

Glu Ala Ala His His Leu Val Leu Thr Ala Ser Asp Gly Gly Lys Pro 
  1               5                  10                  15 

Pro Arg Ser Ser Thr Val Arg Ile His Val Thr Val Leu Asp Thr Asn 
             20                  25                  30 

Asp Asn Ala Pro Val Phe Pro His Pro Ile Tyr Arg Val Lys Val Leu 
         35                  40                  45 

Glu Asn Met Pro Pro Gly Thr Arg Leu Leu Thr Val Thr Ala Ser Asp 
     50                  55                  60 

Pro Asp Glu Gly Ile Asn Gly Lys Val Ala Tyr Lys Phe Arg Lys Ile 
 65                  70                  75                  80 

Asn Glu Lys Gln Thr Pro Leu Phe Gln Leu Asn Glu Asn Thr Gly Glu 
                 85                  90                  95 

Ile Ser Ile Ala Lys Ser Leu Asp Tyr Glu Glu Cys Ser Phe Tyr Glu 
            100                 105                 110 

Met Glu Ile Gln Ala Glu Asp Val Gly Ala Leu Leu Gly Arg Thr Lys 
        115                 120                 125 

Leu Leu Ile Ser Val Glu Asp Val Asn Asp Asn Arg Pro Glu Val Ile 
    130                 135                 140 

Ile Thr Ser Leu Phe Ser Pro Val Leu Glu Asn Ser Leu Pro Gly Thr 
145                 150                 155                 160 

Val Ile Ala Phe Leu Ser Val His Asp Gln Asp Ser Gly Lys Asn Gly 
                165                 170                 175 

Gln Val Val Cys Tyr Thr Arg Asp Asn Leu Pro Phe Lys Leu Glu Lys 
            180                 185                 190 

Ser Ile Gly Asn Tyr Tyr Arg Leu Val Thr Arg Lys Tyr Leu Asp Arg 
        195                 200                 205 

Glu Asn Val Ser Ile Tyr Asn Ile Thr Val Met Ala Ser Asp Leu Gly 
    210                 215                 220 

Thr Pro Pro Leu Ser Thr Glu Thr Gln Ile Ala Leu His Val Ala Asp 
225                 230                 235                 240 

Ile Asn Asp Asn Pro Pro Thr Phe Pro His Ala Ser Tyr Ser Ala Tyr 
                245                 250                 255 

Ile Leu Glu Asn Asn Leu Arg Gly Ala Ser Ile Phe Ser Leu Thr Ala 
            260                 265                 270 

His Asp Pro Asp Ser Gln Glu Asn Ala Gln Val Thr Tyr Ser Val Thr 
        275                 280                 285 

Glu Asp Thr Leu Gln Gly Ala Pro Leu Ser Ser Tyr Ile Ser Ile Asn 
    290                 295                 300 

Ser Asp Thr Gly Val Leu Tyr Ala Leu Gln Ser Phe Asp Tyr Glu Gln 
305                 310                 315                 320 

Ile Arg Asp Leu Gln Leu Leu Val Thr Ala Ser Asp Ser Gly Asp Pro 
                325                 330                 335 

Pro Leu Ser Ser Asn Met Ser Leu Ser Leu Phe Val Leu Asp Gln Asn 
            340                 345                 350 

Asp Asn Ala Pro Glu Ile Leu Tyr Pro Ala Leu Pro Thr Asp Gly Ser 
        355                 360                 365 

Thr Gly Val Glu Leu Ala Pro Arg Ser Ala Glu Arg Gly Tyr Leu Val 
    370                 375                 380 

Thr Lys Val Val Ala Val Asp Arg Asp Ser Gly Gln Asn Ala Trp Leu 
385                 390                 395                 400 

Ser Tyr Arg Leu Leu Lys Ala Ser Glu Pro Gly Leu Phe Ser Val Gly 
                405                 410                 415 

Leu His Thr Gly Glu Val Arg Thr Ala Arg Ala Leu Leu Asp Arg Asp 
            420                 425                 430 

Ala Leu Lys Gln Ser Leu Val Val Ala Val Gln Asp His Gly Gln Pro 
        435                 440                 445 

Pro Leu Ser Ala Thr Val Thr Leu Thr Val Ala Val Ala Asp Ser Ile 
    450                 455                 460 

Pro Glu Val Leu Thr Glu Leu Gly Ser Leu Lys Pro Ser Val Asp Pro 
465                 470                 475                 480 

Asn Asp Ser Ser Leu Thr Leu Tyr Leu Val Val Ala Val Ala Ala Ile 
                485                 490                 495 

Ser Cys Val Phe Leu Ala Phe Val Ala Val Leu Leu Gly Leu Arg Leu 
            500                 505                 510 

Arg Arg Trp His Lys Ser Arg Leu Leu Gln Asp Ser Gly Gly Arg Leu 
        515                 520                 525 

Val Gly Val Pro Ala Ser His Phe Val Gly Val Glu Glu Val Gln Ala 
    530                 535                 540 

Phe Leu Gln Thr Tyr Ser Gln Glu Val Ser Leu Thr Ala Asp Ser Arg 
545                 550                 555                 560 

Lys Ser His Leu Ile Phe Pro Gln Pro Asn Tyr Ala Asp Met Leu Ile 
                565                 570                 575 

Ser Gln Glu Gly Cys Glu Lys Asn Asp Ser Leu Leu Thr Ser Val Asp 
            580                 585                 590 

Phe His Glu Tyr Lys Asn Glu Ala Asp His Gly Gln Val Ser Leu Val 
        595                 600                 605 

Leu Cys Leu Leu Leu Ile Ser Arg 
    610                 615