PATENT ABSTRACT
This invention provides methods of inducing a protective or therapeutic immune response to Helicobacter infection in a mammal by parenterally administering to the mammal one or more Helicobacter antigens and an adjuvant selected from one or more of LT, CT, LTB, and CTB.

PATENT DESCRIPTION
This is a continuation-in-part of U.S. Ser. No. 09/100,258, filed Jun. 19, 1998. 
    
    
     BACKGROUND OF THE INVENTION 
     This invention relates to methods of immunizing against Helicobacter infection. 
     Cholera toxin (CT) and the heat-labile-enterotoxin of  E. coli  (LT) are commonly used as immunological adjuvants for mucosal immunization. The non-toxic B subunit of LT (LTB) has also been shown to have mucosal immunomodulating activity. When delivered mucosally with urease or other Helicobacter antigens, LT and CT each induce immune responses that protect mice against infection with  Helicobacter pylori.    
     Xu-Amano et al. (J. Exp. Med. 178:1309-1320, 1993) showed that mice develop specific serum IgM and IgG (but not IgA) responses following intraperitoneal immunization with tetanus toxin (TT) plus CT. TT alone gave low responses. Hornquist, et al. (Eur. J. Immunol. 23:2136-2143, 1993) showed that CT promotes priming of CD4+ T cells when delivered intravenously with Keyhole Limpet Hemocyanin (KLH). Marinaro et al. (J. Immunol. 155:4621-4629, 1995) showed that CT stimulates production of serum IgE to TT when the antigen and adjuvant are delivered subcutaneously. 
     SUMMARY OF THE INVENTION 
     We have discovered that parenterally delivered LT and LTB are effective adjuvants that enhance the immunoprotective effect of Helicobacter antigens, such as urease. We have also discovered that LT+LTB gives better results that LT alone. 
     Accordingly, the invention provides methods of inducing a protective or therapeutic immune response to Helicobacter infection in a mammal, in which (a) a Helicobacter antigen, and (b) an adjuvant including one or more of (i) LT, (ii) LTB, (iii) CT, and (iv) CTB (e.g., LT+LTB) is parenterally (e.g., subcutaneously, intradermally, intramuscularly, or intravenously) administered to a mammal. 
     The antigen and the adjuvant can be provided together in a solution, or separately. The antigen can be urease or a subunit, enzymatically inactivate derivative, or fragment thereof. The antigen can also be catalase, HspA, HspB, lactoferrin receptor, p76 (SEQ ID NOs:1-22), p32 (SEQ ID NOs:23 and 24), BabA, BabB, AlpA, AlpB, or an immunogenic fragment or derivative thereof. The methods of the invention also include the administration of more than one Helicobacter antigen. 
     The invention provides several advantages. For example, parenteral delivery of LT is advantageous in that the toxin does not contact the intestinal epithelial cells, and thus does not cause diarrhea. Also, the toxicity of parenterally administered LT we observed was limited to injection site swelling, and we observed no toxicity with LTB at high doses (see below), which matched the effectiveness of LT in augmenting protective immunity. Also, our observation of the heightened effect of parenterally administering LT (low dose)+LTB (high dose) minimizes the possibility of any potential side effects. 
     Other features and advantages of the invention are apparent from the following detailed description, the drawings, and the claims. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 is a graph showing gastric urease activity measured under treatment and control conditions. Urease activity in samples of mouse stomach tissue was determined by a calorimetric assay. Individual A 550  values for each mouse (triangles) are shown along with the median value for each group (lines). (See below for further details.) 
     FIG. 2 is a graph showing quantitative culture measurements made under treatment and control conditions. Samples of mouse stomach tissue were homogenized and cultured to determine the number of colony-forming units (CFU) per sample. Individual counts for each mouse (circles) are shown along with the median for each group (lines). (See below for further details.) 
     FIG. 3 is a graph showing anti-urease serum IgG measurements made under treatment and control conditions. Post-immunization sera were tested for IgG antibody against urease by ELISA. IgG levels in ELISA units (EU) per ml for individual mice are shown. 
     FIG. 4 is a graph showing anti-urease salivary IgA measurements made under treatment and control conditions. Post-immunization saliva samples were diluted 1:10 and tested for IgA antibody against urease by ELISA. A 405  values for individual mice are shown. 
     FIG. 5 is a graph showing gastric  H. pylori  colonization in mice following oral or subcutaneous immunization with urease alone or mixed with LT or LTB. Mice were either unimmunized (no treatment) or immunized 3 times with urease plus the adjuvants shown and challenged with  H. pylori  orally 2 weeks after the final immunization. For oral immunization, 25 μg of urease was delivered with 1 μg of LT. For subcutaneous immunization, 10 μg urease was delivered with the dose of adjuvant shown. Symbols show the CFU per quarter antrum of individual mice. The horizontal lines show the median for each group. 
     FIG. 6 is a graph showing titers of IgG to urease in sera of immunized mice. Mice were immunized 3 times orally or subcutaneously with urease plus the adjuvants shown. Serum was collected 1 week after the final immunization and. tested for urease-specific IgG by ELISA. Endpoint titers for individual mice are shown. Samples in which no specific antibody was detected were assigned a titer of 50. The horizontal lines show the median titer for each group. 
     FIG. 7 is a graph showing IgE to urease in sera of immunized mice. Mice were immunized 3 times orally or subcutaneously with urease plus the adjuvants shown. Serum was collected 1 week after the final immunization and tested for urease-specific IgE at a 1/100 dilution by ELISA. Absorbance values for individual mice are shown. The horizontal lines show the median value for each group. 
     FIG. 8 is a graph showing IgA to urease in saliva samples of immunized mice. Mice were immunized 3 times orally or subcutaneously with urease plus the adjuvants shown. Saliva was collected 1 week after the final immunization and tested for urease-specific IgA at a 1/10 dilution by ELISA. Absorbance values for individual mice are shown. The horizontal lines show the median value for each group. 
     FIG. 9 is a graph showing urease-specific IgE in serum and IgA in saliva of mice immunized 3 times subcutaneously with urease adsorbed to alum adjuvant. Serum and saliva were collected 1 week after the final immunization and tested for urease-specific antibody by ELISA. Absorbance values for individual mice are shown. The horizontal lines show the median value for each group. 
     FIG. 10 is a graph showing gastric urease activity measured under treatment and control conditions. Urease activity in samples of mouse stomach tissue was determined by a calorimetric assay. Individual A 550  values for each mouse (circles) are shown along with the median value for each group (lines). (See below for further details.) 
     FIG. 11 is a graph showing quantitative culture measurements made under treatment and control conditions. Samples of mouse stomach tissue were homogenized and cultured to determine the number of colony-forming units (CFU) per sample. Individual counts for each mouse (circles) are shown along with the median for each group (lines). (See below for further details.) 
    
    
     DETAILED DESCRIPTION 
     The methods and compositions of the invention employ several components and techniques, which are described in greater detail as follows. 
     Antigens 
     Preferred antigens for use in the invention are Helicobacter (e.g.,  H. pylori  or  H. felis ) proteins or other components (e.g., lipopolysaccharides or carbohydrates) that either are purified from bacterial cultures or are produced using standard recombinant or chemical synthetic methods. Preferred antigens are proteins or portions of proteins (i.e., peptides or polypeptides). Methods for identifying immunogenic fragments of polypeptide antigens are known in the art, and can be employed in preparing antigens for use in the methods of the invention (Sturniolo et al., Nature Biotechnology, “Generation of Tissue-Specific and Promiscuous HLA Ligand Databases Using DNA Microarrays and Virtual HLA Class II Matrices,” June 1999). Additional antigens that can be used in the invention are whole bacteria and non-purified protein preparations, such as Helicobacter lysates. 
     The antigens used in the invention can be produced as fusion proteins, i.e., polypeptides containing amino acid sequences corresponding to two or more proteins (or fragments thereof), which are normally separate proteins, linked together by a peptide bond(s). Fusion proteins generally are synthesized by expression of a hybrid gene containing nucleotides encoding each of the individual polypeptides that make up the fusion protein. An example of an antigenic fusion protein included in the invention is one that contains a CT or an LT toxin adjuvant (e.g., toxin A or B subunit, or a fragment or derivative thereof having adjuvant activity) fused to an  H. pylori  antigen, e.g., urease. Another type of fusion protein included in the invention consists of an antigen fused to a polypeptide (e.g., glutathione S-transferase (GST)) that facilitates purification of the fusion protein. Proteins used as antigens in the invention can also be covalently coupled or chemically cross-linked to adjuvants using standard methods. 
     The most preferred  H. pylori  antigens for use in the invention are urease and derivatives thereof. Most preferred are enzymatically inactive, recombinant multimeric urease complexes, produced as described in Lee et al., WO 96/33732, which is hereby incorporated by reference. A number of other immunogenic  H. pylori  antigens can also be administered according to the invention, e.g., catalase (WO 95/27506), HspA and HspB (WO 94/26901), lactoferrin receptor (WO 97/13784), p76 (SEQ ID NOs:1-22; WO 97/12908), p32 (SEQ ID NOs:23 and 24; WO 97/12909), BabA and BabB (WO 97/47646), AlpA (WO 96/41880), AlpB (WO 97/11182), as well as the antigens described in WO 96/38475, WO 96/40893, WO 97/19098, WO 97/37044, and WO 98/18323. The immunogenic antigens can be used alone (with the adjuvant) or in “cocktails” of two or more antigens. 
     LT and CT Adjuvants 
     Any of the known LT or CT adjuvants or variants thereof can be used in the invention. Although the native form can be used (see, e.g., Clements et al., Vaccine 6:269, 1988), and exhibits strong adjuvant activity, it is somewhat toxic, and therefore less toxic mutants are preferred. Several such mutants, having demonstrated adjuvant activity, are described in WO 93/13202, WO 95/17211, and WO 96/06627, which are hereby incorporated by reference. The low-toxicity B subunit (LTB), described in U.S. Pat. Nos. 5,308,835, 5,079,165, 4,808,700, and 5,182,109, which are hereby incorporated by reference, is also efficacious. 
     The invention can also employ LT or CT toxoids as adjuvants. A toxoid is a toxin that has been treated so as to destroy or decrease its toxic properties, but to retain adjuvant activity. Toxoids included in the invention are made using standard methods including, but not limited to, chemical (e.g., formaldehyde or glutaraldehyde) treatment, protease cleavage, and recombinant methods (e.g., by making fragments or mutations (e.g., point mutations) of the toxin(s)). DNA encoding LT or CT toxin subunit or toxoid in an appropriate expression vector can also be used. CT and LT are further described, for example, by Spangler (Microbiological Reviews 56(4):622-647, 1992). 
     Vaccine Formulation 
     Generally, the  H. pylori  antigen(s) and adjuvant are admixed together in a pharmaceutically acceptable carrier, e.g., water, saline, or phosphate-buffered saline. The concentration of  H. pylori  antigen in the composition preferably is between 10 μg and 1 mg, advantageously from 25 μg to 500 μg, preferably from 50 μg to 200 μg, and most preferably a single dose contains about 100 μg antigen. The concentration of LT or CT adjuvant in the composition preferably is between 1 μg and 100 μg, and the concentration of LTB or CTB subunit or toxoid is preferably between 1 μg and 1 μg, for example, between 10 μg and 50 μg. 
     Administration 
     The compositions of the invention are administered parenterally; i.e., the composition is injected subcutaneously, intramuscularly, intravenously, intradermally, or by any other non-mucosal modality. The LT or CT adjuvant can be administered in an encapsulated form or in an unencapsulated form (i.e., in solution). 
     The methods of the invention can be used both for treatment and prevention of  H. pylori  infection. For prevention, the composition is injected into the patient at intervals of one week to six months for a period of between one and six months, at a dosage of 0.05 to 5 mg/kg  H. pylori  antigen. Where the patient has an  H. pylori  infection that is to be treated, injections at intervals of one week to six months, for one to six months, are administered, for 0.05 to 5 mg/kg  H. pylori antigen. Antibiotics can be administered as an adjunct to the immunotherapy of the invention. 
     The following experimental results support the methods of the present invention. 
     Results 
     Characterization of recombinant LT and LTB. LT and LTB were tested for toxicity using a Y-1 cell-rounding assay (Chapman et al., J. Med. Microbiol. 18:399-403, 1984). LT was tested before and after treatment with trypsin, which activates the toxin by cleavage of the A subunit. The dose of untreated LT required for 50% cell rounding (ED50) was 0.2 ng/ml. Following trypsin treatment, the ED50 fell to 0.1 ng/ml, indicating that approximately 50% of the LT was active prior to trypsin treatment. LTB had no activity in the assay when tested at concentrations up to 100 μg/ml. 
     Adjuvant effects of parenterally delivered LT and LTB for protection against  H. pylori  challenge. Mice were immunized subcutaneously or intradermally with 10 μg of  H. pylori  urease mixed with 5 ng to 5 μg of recombinant LT or 5 μg to 50 μg of recombinant LTB (see Table 1). As a positive control, mice were immunized orally or intragastrically with 25 μg of urease plus 1 μg LT, a regimen shown previously to protect against  H. pylori  challenge (Ermak et al., J. Exp. Med. 188:2277-2288, 1998). A schedule of 3 immunizations, with a two-week interval between doses was used. Mice injected subcutaneously with the two higher doses of LT (0.5 and 5 μg) developed swelling at the injection site that was still present two weeks after the first immunization. As a result, the second dose was omitted in these 2 groups and a single boosting immunization was given 4 weeks after the first dose, at which point the swelling had subsided. Injection site swelling consisted of a raised area approximately 1 cm in diameter with no skin color change or necrosis apparent. No other signs of toxicity were observed. Mice injected intradermally with 0.5 or 5 μg of LT also experienced injection site swelling. Because swelling did not subside as quickly in these mice, further immunization was not performed and the mice were removed from the study. Two weeks after the final immunization, mice were challenged intragastrically with mouse-adapted  H. pylori  strain X47-2AL. Gastric colonization was assessed two weeks after challenge by determining urease activity and bacterial CFU. 
     Gastric urease activity results are shown in FIG. 1. A 550  values for individual mice and the median for each group are shown. Some groups contain fewer than 10 data points, due to a technical problem with the assay. Mice in the unimmunized group were clearly infected (A 550 ≧0.20), with a median A 550  of 0.67. Mice immunized intragastrically with urease plus LT (positive control) had a median A 550  of 0.21, with substantial reductions in gastric  H. pylori  colonization in all mice. A similar effect was seen in mice immunized subcutaneously with urease plus LT at the two highest doses (5 and 0.5 μg). The lower doses of LT (50 and 5 ng) were somewhat less effective. Intradermal immunization with urease and LT result in similar levels of protection. Subcutaneous immunization with urease plus 50 μg LTB resulted in the lowest median A 550  value of any of the groups. 
     Results of quantitative culture of gastric tissue mirrored those of the urease assay (FIG.  2 ). The highest subcutaneous doses of LT and LTB resulted in a level of protection equal to that afforded by intragastric immunization. Lower doses had lesser effects. More specifically, the median level of gastric  H. pylori  colonization in unimmunized mice was 2.9×10 5  CFU/stomach sample (FIG.  2 ). In mice immunized orally with urease plus LT, the median level was 9.1×10 3  CFU/sample, a significant reduction in colonization (P=0.0004) that was consistent with previous experiments using the X47-2AL challenge strain (26, 38). Subcutaneous delivery of urease with the highest dose of LT (5 μg) was as effective at reducing infection as oral immunization, resulting in a significantly reduced median  H. pylori  level of 8.3×10 3  CFU/sample (FIG.  2 ). Subcutaneous delivery of urease with lower doses of LT reduced gastric colonization to a lesser, but significant (P&lt;0.05) extent. Intradermal injection of urease with 5 ng or 50 ng of LT was similar in effectiveness to subcutaneous delivery of the same doses. Subcutaneous delivery of urease with 50 μg of LTB reduced the median level of  H. pylori  to 3.2×10 3  CFU/sample, the lowest level in any of the groups. A lesser but significant effect (P=0.0006) was seen when only 5 μg of LTB was used. 
     All mice developed IgG to urease in serum following immunization (FIG.  3 ). Responses in parenterally immunized mice were higher than those of intragastrically immunized mice. In groups receiving LT parenterally, there was a trend towards lower IgG responses with lower doses. Mice immunized with urease plus LTB had somewhat lower responses than mice immunized with urease plus LT. 
     Salivary IgA to urease was highest in the intragastrically immunized group, although 3 of 10 mice did not respond (FIG.  4 ). Other immunized groups had a range of IgA responses. In mice immunized with urease plus LT subcutaneously, the highest responders were in the groups treated with the two lowest doses of LT (50 and 5 ng). Intradermal immunization with urease plus 50 or 5 ng LT stimulated salivary IgA in most mice. LTB immunization resulted in IgA responses primarily in the lower dose group. 
     The results show that mucosally or parenterally delivered LT acts as an effective adjuvant for immunization of mice against  H. pylori  infection. Surprisingly, parenteral LT stimulated a secretory IgA response against urease. In previous experiments, we found that subcutaneous injection of urease, alone or adsorbed to alum adjuvant, elicited little or no salivary IgA response. 
     Adjuvant effects of orally delivered LTB and parenterally delivered LT-LTB mixture. A second experiment, using the same immunization schedule and challenge procedure as described above, was designed to examine several questions regarding the adjuvant activities of LT and LTB with urease, including whether LTB was active orally, whether subcutaneously delivered urease was protective without LT or LTB, and whether a combination of LT and LTB was more effective than either molecule alone. As shown in FIG. 5, oral delivery of urease plus 1 μg LT reduced gastric colonization to a median level of 1.5×10 4  CFU/sample from a median of 7.9×10 4  CFU/sample in untreated mice (P=0.02). Oral delivery of urease with 50 μg of LTB, however, had no effect on colonization (P=0.82). Subcutaneous immunization with urease alone reduced the median  H. pylori  level slightly from that of unimmunized mice (P=0.02) and addition of LT or LTB enhanced the protective effect (P≦0.01). As in the previous experiment, LTB was more effective at the higher dose (50 μg) than at the lower dose (5 μg). Administration of 50 μg of LTB in the absence of urease had no effect on colonization. The greatest effect was seen when a mixture of 50 μg LTB and 10 ng LT was used as adjuvant for subcutaneous immunization. In this group, colonization was reduced to 2.1×10 3  CFU/sample (P=0.0002). 
     Antibody responses to urease following parenteral delivery of urease with LT or LTB adjuvants. Serum and saliva collected after immunization, but before  H. pylori  challenge, were examined to determine the effects of different adjuvants and routes of immunization on the magnitude and type of antibodies elicited against urease. IgG to urease was not detected in the serum of unimmunized mice when tested at a 1/100 dilution. Oral immunization with urease plus LT elicited low to moderate titers of urease-specific IgG (FIG.  6 ). IgG titers were similar when mice were immunized orally using LTB as adjuvant. In serum from subcutaneously immunized mice, IgG levels in all groups were higher than those obtained with oral immunization and responses were more uniform within each group. Subcutaneous immunization with urease alone resulted in IgG titers of greater than 5 log 10 , while the addition of LT or LTB increased titers several fold (P=0.06 and P&lt;0.005, respectively, comparing the LT and LTB groups against the no-adjuvant group). As with protection, co-administration of LT and LTB had an additive effect, resulting in a urease-specific IgG titer of 6.9 log 10 , a 20-fold increase over the median titer in mice immunized with urease in the absence of adjuvant (P=0.002). 
     Analysis of serum IgG1 and IgG2a levels showed differences in the quality of the urease-specific IgG response among different groups (Table 2). In comparison to subcutaneous immunization, oral immunization tended to elicit lower levels of both IgG1 and IgG2a and a lower IgG1/IgG2a ratio, whether LT or LTB was delivered with urease. In contrast, subcutaneous LT and LTB elicited IgG responses to urease that were qualitatively different from each other. Subcutaneous delivery of urease alone elicited strong IgG1 and IgG2a responses, and the levels of both isotypes were enhanced by addition of LT or LTB. However, LT stimulated a greater increase in IgG1 than in IgG2a, while LTB stimulated a greater increase in IgG2a than in IgG1. When LT and LTB were delivered together, the effect of LTB appeared to be dominant, since the median IgG2a titer of 6.7 log 10 , was nearly 80-fold higher than that of mice receiving urease without adjuvant and the highest of any group. 
     Serum IgE to urease was generated only by parenteral immunization (FIG.  7 ). Subcutaneous immunization with urease alone stimulated a low level IgE response in most mice. The addition of LT adjuvant increased IgE levels, although responses were highly variable. The median IgE response in mice receiving LTB adjuvant was only slightly higher than that of mice receiving no adjuvant. LT and LTB together led to levels that were somewhat higher than those seen with LTB alone. 
     IgA to urease in saliva was greatest when mice were immunized orally and LT adjuvant was used, although not all mice had a detectable IgA response (FIG.  8 ). Delivery of urease orally with LTB or subcutaneously without adjuvant stimulated only low levels of specific IgA in saliva. LT stimulated low levels of specific IgA in the saliva of most mice when delivered subcutaneously with urease, while LTB had a similar effect but also induced moderate levels of salivary IgA in several mice. When LT and LTB were delivered together, specific salivary IgA was not increased over the level seen when mice were immunized with urease alone. 
     Antibody responses to urease following parenteral delivery of urease with alum adjuvant. To compare the adjuvant activities of LT and LTB with a more conventional parenteral adjuvant, mice were immunized subcutaneously with urease adsorbed to alum (aluminum hydroxide) adjuvant. As in the experiments described above, mice were immunized 3 times and blood and saliva were collected 1 week after the final immunization. This regimen was shown previously to reduce colonization of the stomach following  H. pylori  challenge, but was less effective than oral immunization with urease plus LT (Ermak et al., J. Exp. Med. 188:2277-2288, 1998). The titer of specific IgG in serum following immunization with urease adsorbed to alum ranged from 5.7 to 7.1 log 10 , with a median of 6.3 log 10 . This was the same approximate level of serum IgG elicited by subcutaneous delivery of urease plus LT or LTB. The median titers of IgG1 and IgG2a in serum following urease plus alum immunization were 6.6 and 5.5, respectively (Table 2). IgG1/IgG2a ratios were relatively high, ranging from 3.9 to 53.2. This range was similar to that obtained with subcutaneous delivery of urease alone or with LT. IgE levels were higher with alum than with LT or LTB as adjuvant, with A 405  values for urease-specific IgE exceeding 2.0 in over half of the mice that received alum (FIG.  9 ). Only slight salivary IgA responses were stimulated with alum as adjuvant (FIG.  9 ). 
     Additional data supporting these findings is shown in FIGS. 10 and 11. In these experiments, groups of 10 mice each were immunized on days 0, 14, and 28, as follows: no antigen, no adjuvant (group 1); 25 μg urease, 1 μg LT, orally (group 2); 25 μg urease, 50 μg LTB, orally (group 3); 10 μg urease, no adjuvant, s.c. (group 4); 10 μg urease, 10 ng LT, s.c. (group 5); 10 μg urease, 5 μg LTB, s.c.(group 6); 10 μg urease, 50 μg LTB, s.c. (group 7); 10 μg urease, 10 ng LT, 50 μg LTB, s.c. (group 8); and no antigen, 50 μg LTB, s.c. (group 9). The groups were challenged with 1×10 7    H. pylori  CFU at day 42. At day 56, mice were sacrificed and the gastric colonization was assessed by testing stomach samples for urease activity (Jatrow test) and  H. pylori  culture. As is shown in FIGS. 10 and 11, 10 ng LT and 50 μg LTB given subcutaneously with urease (groups 5 and 7) similarly lead to a significant reduction of the infection rate, compared to controls (groups 1 and 9). These results are as good as those obtained with urease +1 μg LT given orally (group 5); 10 ng LT +50 μg LTB (group 7), give even better results. 
     The results described above were obtained using the following materials and methods. 
     Materials and Methods 
     Antigens and adjuvants. Recombinant  H. pylori  urease was expressed in  E. coli  strain ORV214 and purified by anion-exchange and gel filtration chromatography as described previously (Lee et al., J. Infect. Dis. 172:161-172, 1995). Endotoxin concentration, as determined by the Limulus amoebocyte lysate assay, was reduced to 1.5 ng/mg urease using a Sartobind matrix (Sartorius Corporation, N.Y.). Recombinant LT was obtained from Berna Products Corp. (Coral Gables, Fla.). For trypsin cleavage, 100 μg of LT was mixed with 1 μg of bovine pancreas trypsin (Sigma Chemical Co., St. Louis, Mo.) in 200 μl phosphate buffered saline (PBS) and incubated for 60 minutes at 37° C. Enzymatic activity was stopped by addition of 100 μg of soybean trypsin inhibitor (Sigma). Recombinant LTB was cloned and expressed in  E. coli.    
     B subunit was purified from lysates by galactose affinity chromatography which yielded a product consisting of greater than 90% pentameric LTB. Endotoxin was removed using a Sartobind matrix. Purified LTB was lyophilized and stored. 
     Y-1 cell rounding assay. Toxicity of LT and LTB was assessed with a modified cell rounding assay (Chapman et al., J. Med. Microbiol. 18:399-403, 1984). In brief, 96-well flat bottom tissue culture plates were seeded with Y-1 mouse adrenal cells at 2×10 4  cells/well in minimum essential medium supplemented with 10% fetal bovine serum. Following incubation at 37° C. in 5% CO 2  for ≧2 hours to allow spreading of cells on the substrate, medium was removed from wells and replaced with medium containing serial two-fold dilutions of LT or LTB and plates returned to the incubator. After overnight incubation at 37° C., the cells were viewed with an inverted microscope. The toxin potency was defined as the lowest concentration at which ≧50% of cells were rounded (50% effective dose or ED50). 
     Immunization and sampling. Procedures involving animals were approved by the Institutional Animal Care and Use Committee of OraVax, Inc. Specific pathogen-free, 6 to 8 week-old female Swiss-Webster mice were purchased from Taconic Laboratories (Germantown, N.Y.). Mice were immunized with urease via the oral, subcutaneous or intradermal routes. Three doses were given with a 2-week interval between doses. For oral immunization, 25 μg of urease and 1 μg of LT in a volume of 25 μl was pipetted into the mouth. For subcutaneous immunization, 10 μg of urease was injected in the lower back, with or without LT, LTB or aluminum hydroxide (alum) adjuvant, in a volume of 100 μl. Equal volumes of alum (Rehydragel, Reheis, Inc., Berkeley Heights N.J.) at 2 mg/ml and urease at 100 μg/ml were mixed 30-60 minutes prior to injection. For intradermal immunization, a patch of skin on the back was shaved, the mice were anesthetized by isoflurane inhalation, and 10 μg urease with LT or LTB adjuvant was delivered via a 30 gauge needle in a volume of 50 μl. 
     Blood and saliva were sampled approximately 1 week after the third immunization. Blood was collected from the retro-orbital sinus while mice were under isoflurane inhalation anesthesia, and saliva was collected from the mouth with a micropipette after intraperitoneal injection of 70-100 μg of pilocarpine. 
     Challenge and analysis of colonization. Approximately 2 weeks after the final immunization, mice were challenged intragastrically with a single dose of 1×10 7  live streptomycin-resistant  H. pylori  strain X47-2AL (Kleanthous et al., Infect. &amp; Immun. 66:2879-2886, 1998). Challenge bacteria were grown first on Mueller-Hinton agar plates containing 10% sheep blood and transferred to Brucella broth as described previously (Kleanthous et al., Infect. &amp; Immun. 66:2879-2886, 1998). The challenge dose was delivered intragastrically in a volume of 100 μl using a blunt-tipped feeding needle. Mice were sacrificed 2 weeks after challenge to assess gastric colonization by  H. pylori . The stomach was removed, rinsed with 0.9% NaCl solution, and cut open along the lesser and greater curvatures. One quarter of the antrum was placed into 1 ml of Brucella broth supplemented with 5% calf serum and disrupted using a Dounce homogenizer fitted with a loose pestle. Tenfold dilutions of homogenate were inoculated onto Mueller-Hinton agar containing 10% sheep blood, 5 μg/ml amphotericin B, 5 μg/ml trimethoprim, 10 μg/ml vancomycin, 10 U/ml polymyxin B sulfate, and 50 μg/ml streptomycin. Plates were incubated at 37° C. in 7% CO 2  and colonies counted 5-7 days later. 
     Antibody analysis. Antibody responses in serum and saliva were measured by enzyme-linked immunosorbent assay (ELISA). Flat-bottom 96-well plates were coated overnight at 4° C. with 0.5 μg urease per well in 100 μl of 0.1 M carbonate buffer, pH 9.6. Wells were washed with PBS containing 0.05% Tween 20 (PBS-Tween), and PBS-Tween containing 2.5% nonfat dry milk (blocking buffer) was added to block nonspecific binding. Blocking buffer was used as diluent for test samples and antibody conjugates. Samples and reagents were added to wells in 100 μl volumes and wells were washed with PBS-Tween between steps. For IgG, IgG1, and IgG2a determination, serum was diluted 1/100 and then further diluted with a series of five-fold dilutions. After removing blocking buffer, 100 μl of each dilution was added in duplicate and plates were incubated for 60 minutes at 28° C. Biotin-conjugated goat anti-mouse IgG, IgG1, or IgG2a antibodies (Southern Biotechnology Associates, Birmingham Ala.), each diluted 1/1000, were added to wells next and plates were incubated for. 60 minutes at 28° C. Streptavidin-alkaline phosphatase (Calbiochem, La Jolla Calif.) diluted 1/500 was added next and plates were incubated for 30 minutes at 28° C. For the final step, 100 μl of 1 mg/ml p-nitrophenyl phosphate substrate (Sigma) in 1 M diethanolamine buffer, pH 9.6 containing 5 mM MgCl 2  was added. Plates were incubated for 20 minutes at room temperature and the A 405  read with a Vmax microplate reader (Molecular Devices, Menlo Park, Calif.). The average absorbance value of duplicate wells was plotted and a curve was fit to the data points using the power function of Cricket Graph III software (Computer Associates International, Inc., Islandia N.Y.) running on an Apple Macintosh computer. The titer of each sample was defined as the reciprocal of the dilution corresponding to an A 405  of 0.1, which was approximately 3 times the background absorbance for normal mouse serum at a 1/100 dilution. Samples with an A 405  less than 0.1 at a 1/100 dilution were assigned a titer of 50. For determination of salivary IgA levels, saliva was tested at a single dilution of 1/10 and the results are reported as the average A 405  for duplicate wells. The assay procedure for IgA determination was otherwise as described above except that goat anti-mouse IgA biotin conjugate (Southern Biotechnology) was used to detect bound salivary IgA antibodies. 
     For quantitation of IgE to urease, a capture ELISA was used. Flat-bottom 96-well plates were coated overnight with 100 μl of rat monoclonal antibody clone 23G3 against mouse IgE (Southern Biotech) at a concentration of 1 μ/ml. Wells were washed and blocked with nonfat milk as described above and 100 μl of mouse serum, diluted 1/100 was added to wells in duplicate. Following incubation for 1 hour at 28° C., the plates were washed and urease, at 2.5 μg/ml, was added to wells. Plates were again incubated for 1 hour at 28° C. In subsequent steps, wells were treated with rabbit anti-urease diluted 1/4000 and goat anti-rabbit IgG conjugated to alkaline phosphatase at a 1/2000 dilution. Plates were incubated for 1 hour at 28° C. at each step. Addition of substrate solution and measurement of absorbance was done as described above. 
     
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Immunization protocol 
               
             
          
           
               
                   
                 Urease 
                   
                 Adjuvant 
                 Delivery 
                 Delivery 
               
               
                 Group 
                 dose 
                 Adjuvant 
                 dose 
                 route 
                 schedule 
               
               
                   
               
               
                 1 
                 none 
                 none 
                 — 
                 — 
                 — 
               
               
                 2 
                 25 μg 
                 LT 
                   1 μg 
                 i.g. 
                 days 0, 14, 28 
               
               
                 3 
                 10 μg 
                 LT 
                   5 μg 
                 s.c. 
                 days 0, 28 
               
               
                 4 
                 10 μg 
                 LT 
                 0.5 μg 
                 s.c. 
                 days 0, 28 
               
               
                 5 
                 10 μg 
                 LT 
                  50 ng 
                 s.c. 
                 days 0, 14, 28 
               
               
                 6 
                 10 μg 
                 LT 
                   5 ng 
                 s.c. 
                 days 0, 14, 28 
               
               
                 7 
                 10 μg 
                 LT 
                  50 ng 
                 i.d. 
                 days 0, 14, 28 
               
               
                 8 
                 10 μg 
                 LT 
                   5 ng 
                 i.d. 
                 days 0, 14, 28 
               
               
                 9 
                 10 μg 
                 LTB 
                  50 μg 
                 s.c. 
                 days 0, 14, 28 
               
               
                 10  
                 10 μg 
                 LTB 
                   5 μg 
                 s.c. 
                 days 0, 14, 28 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Serum IgG1 and IgG2a antibody responses following immunization with 
               
               
                 urease alone or urease with LT, LTB, or alum adjuvants. 
               
               
                 Median log 10  titer (range) a   
               
             
          
           
               
                   
                   
                   
                   
                   
                 IgG1/IgG2a ratio 
               
               
                 Adjuvant 
                 Antigen 
                 Route b   
                 IgG1 
                 IgG2a 
                 (range) c   
               
               
                   
               
               
                  1 μg LT 
                 25 μg urease 
                 oral 
                 3.1 (1.7-4.5) 
                 4.3 (1.7-5.2) 
                 0.2 (0.01-1.6) 
               
               
                 50 μg LTB 
                 25 μg urease 
                 oral 
                 2.2 (1.7-4.7) 
                 3.5 (1.7-4.5) 
                 0.03 (0.01-5.2)  
               
               
                 None 
                 10 μg urease 
                 s.c. 
                 5.7 (5.5-6.3) 
                 4.8 (3.9-5.4) 
                  8.3 (3.9-56.7) 
               
               
                 10 ng LT 
                 10 μg urease 
                 s.c. 
                 6.7 (6.3-7.2) 
                 5.3 (4.7-6.1) 
                  30.1 (1.9-257.7) 
               
               
                  5 μg LTB 
                 10 μg urease 
                 s.c. 
                 6.3 (5.8-6.8) 
                 5.8 (5.2-6.4) 
                  4.7 (0.2-8.0) 
               
               
                 50 μg LTB 
                 10 μg urease 
                 s.c. 
                 6.5 (6.0-6.9) 
                 6.2 (5.5-6.5) 
                  2.1 (0.7-5.8) 
               
               
                 10 ng LT + 
                 10 μg urease 
                 s.c. 
                 6.8 (6.2-7.2) 
                 6.7 (6.2-6.9) 
                  1.0 (0.6-4.0) 
               
               
                 50 μg LTB 
               
               
                 Alum 
                 10 μg urease 
                 s.c. 
                 6.6 (6.0-7.0) 
                 5.5 (4.8-5.8) 
                  11.8 (3.9-53.2) 
               
               
                   
               
               
                   a Titers ofurease-specific IgG1 and IgG2a were determined by ELISA. Titers are defined as 1/dilution of serum giving an A 405  of 0.1. Samples that were negative at the lowest dilution tested (1/100) were assigned a titer of 50 (1.7 log 10 ).  
               
               
                   b Adjuvant and antigen were mixed and delivered by the oral or subcutaneous (s.c.) routes.  
               
             
          
         
       
     
     All publications and patent applications mentioned herein are incorporated by reference. Other embodiments are within the following claims. 
     
       
         
           
             24 
           
           
             1 
             2798 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (328)...(2451) 
             
             
               sig_peptide 
               (328)...(385) 
             
           
            1
tggtcctggc attccgaggt tcgaatcctt gcaccccagc catttttcct tattttttgg     60
cgcggagtag agcagtccgg tagctcgttg ggctcataac ccaaaggtca gtggttcaaa    120
tccattctcc gcaaccaatc ctttaaacca caccaccacc aaacgaacca aacgaaacaa    180
aaagcatcaa aatcaaaaaa atgacaaaat ttttaagaaa atgacaaaaa aaaaaaaaac    240
gattttatgc tatattaacg aaatcttgtg ataagatctt attcttttaa aagacttatc    300
taaccatttt aatttcaagg agaaaac atg aaa aaa acc ctt tta ctc tct ctc    354
                              Met Lys Lys Thr Leu Leu Leu Ser Leu
                                              -15
tct ctc tct ctc tcg ttt ttg ctc cac gct gaa gac gac ggc ttt tac      402
Ser Leu Ser Leu Ser Phe Leu Leu His Ala Glu Asp Asp Gly Phe Tyr
-10                  -5                 1               5
aca agc gtg ggc tat caa atc ggt gaa gcc gct caa atg gtg aaa aac      450
Thr Ser Val Gly Tyr Gln Ile Gly Glu Ala Ala Gln Met Val Lys Asn
             10                  15                  20
acc aaa ggc att caa gag ctt tca gac aat tat gaa aag ctg aac aat      498
Thr Lys Gly Ile Gln Glu Leu Ser Asp Asn Tyr Glu Lys Leu Asn Asn
         25                  30                  35
ctt ttg aat aat tac agc acc cta aac acc ctt atc aaa ttg tcc gct      546
Leu Leu Asn Asn Tyr Ser Thr Leu Asn Thr Leu Ile Lys Leu Ser Ala
     40                  45                  50
gat ccg agc gcg att aac gac gca agg gat aat cta ggc tca agc tct      594
Asp Pro Ser Ala Ile Asn Asp Ala Arg Asp Asn Leu Gly Ser Ser Ser
 55                  60                  65                  70
agg aat ttg ctt gat gtc aaa acc aat tcc ccc gcg tat caa gcc gtg      642
Arg Asn Leu Leu Asp Val Lys Thr Asn Ser Pro Ala Tyr Gln Ala Val
                 75                  80                  85
ctt tta gca ctc aat gct gca gtg ggg ttg tgg caa gtt aca agc tac      690
Leu Leu Ala Leu Asn Ala Ala Val Gly Leu Trp Gln Val Thr Ser Tyr
             90                  95                 100
gct ttt act gct tgt ggt cct ggc agt aac gag aat gcg aat gga ggg      738
Ala Phe Thr Ala Cys Gly Pro Gly Ser Asn Glu Asn Ala Asn Gly Gly
        105                 110                 115
atc caa act ttt aat aat gtg cca gga caa gat acg acg acc atc act      786
Ile Gln Thr Phe Asn Asn Val Pro Gly Gln Asp Thr Thr Thr Ile Thr
    120                 125                 130
tgc aat tcg tat tat gag cca gga cat ggt ggg cct ata tcc act gca      834
Cys Asn Ser Tyr Tyr Glu Pro Gly His Gly Gly Pro Ile Ser Thr Ala
135                 140                 145                 150
aat tat gcg aaa atc aat caa gcc tat caa atc atc caa aag gct ttg      882
Asn Tyr Ala Lys Ile Asn Gln Ala Tyr Gln Ile Ile Gln Lys Ala Leu
                155                 160                 165
aca gcc aat gga gct aat gga gat ggg gtc ccc gtt tta agc aac acc      930
Thr Ala Asn Gly Ala Asn Gly Asp Gly Val Pro Val Leu Ser Asn Thr
            170                 175                 180
act aca aaa ctt gat ttc act atc aat gga gac aaa aga acg ggg ggc      978
Thr Thr Lys Leu Asp Phe Thr Ile Asn Gly Asp Lys Arg Thr Gly Gly
        185                 190                 195
aaa cca aat aca cct gaa aag ttc cca tgg agt gat ggg aaa tat att     1026
Lys Pro Asn Thr Pro Glu Lys Phe Pro Trp Ser Asp Gly Lys Tyr Ile
    200                 205                 210
cac acc caa tgg att aac aca ata gta aca cca aca gaa aca aat atc     1074
His Thr Gln Trp Ile Asn Thr Ile Val Thr Pro Thr Glu Thr Asn Ile
215                 220                 225                 230
aac aca gaa aat aac gct caa gag ctt tta aaa caa gcg agc atc att     1122
Asn Thr Glu Asn Asn Ala Gln Glu Leu Leu Lys Gln Ala Ser Ile Ile
                235                 240                 245
atc act acc cta aat gag gca tgc cca aac ttc caa aat ggt ggt aga     1170
Ile Thr Thr Leu Asn Glu Ala Cys Pro Asn Phe Gln Asn Gly Gly Arg
            250                 255                 260
agt tat tgg caa ggg ata agc ggc aat ggg aca atg tgc ggg atg ttt     1218
Ser Tyr Trp Gln Gly Ile Ser Gly Asn Gly Thr Met Cys Gly Met Phe
        265                 270                 275
aag aat gaa atc agc gcg atc caa ggc atg atc gct aac gct caa gaa     1266
Lys Asn Glu Ile Ser Ala Ile Gln Gly Met Ile Ala Asn Ala Gln Glu
    280                 285                 290
gct gtc gcg caa agc aaa atc gtt agt gaa aac gcg caa aat caa aac     1314
Ala Val Ala Gln Ser Lys Ile Val Ser Glu Asn Ala Gln Asn Gln Asn
295                 300                 305                 310
aac ttg gat act gga aaa cca ttc aac cct tac acg gac gcc agc ttt     1362
Asn Leu Asp Thr Gly Lys Pro Phe Asn Pro Tyr Thr Asp Ala Ser Phe
                315                 320                 325
gcg caa agc atg ctc aaa aac gct caa gcg caa gca gag att tta aac     1410
Ala Gln Ser Met Leu Lys Asn Ala Gln Ala Gln Ala Glu Ile Leu Asn
            330                 335                 340
caa gcc gaa caa gta gta aaa aac ttt gaa aaa atc cct aca gcc ttt     1458
Gln Ala Glu Gln Val Val Lys Asn Phe Glu Lys Ile Pro Thr Ala Phe
        345                 350                 355
gta tca gac tct tta ggg gtg tgt tat gaa gtg caa ggg ggt gag cgt     1506
Val Ser Asp Ser Leu Gly Val Cys Tyr Glu Val Gln Gly Gly Glu Arg
    360                 365                 370
agg ggc acc aat cca ggt cag gta act tct aac act tgg gga gcc ggt     1554
Arg Gly Thr Asn Pro Gly Gln Val Thr Ser Asn Thr Trp Gly Ala Gly
375                 380                 385                 390
tgc gcg tat gtg aaa caa acc ata acg aat tta gac aac agc atc gct     1602
Cys Ala Tyr Val Lys Gln Thr Ile Thr Asn Leu Asp Asn Ser Ile Ala
                395                 400                 405
cac ttt ggc act caa gag cag cag ata cag caa gcc gaa aac atc gct     1650
His Phe Gly Thr Gln Glu Gln Gln Ile Gln Gln Ala Glu Asn Ile Ala
            410                 415                 420
gac act cta gtg aat ttc aaa tct aga tac agc gaa tta ggc aac acc     1698
Asp Thr Leu Val Asn Phe Lys Ser Arg Tyr Ser Glu Leu Gly Asn Thr
        425                 430                 435
tat aac agc atc acc acc gcg ctc tcc aaa gtc cct aac gcg caa agc     1746
Tyr Asn Ser Ile Thr Thr Ala Leu Ser Lys Val Pro Asn Ala Gln Ser
    440                 445                 450
ttg caa aac gtg gtg agc aaa aag aat aac ccc tat agc cct caa ggc     1794
Leu Gln Asn Val Val Ser Lys Lys Asn Asn Pro Tyr Ser Pro Gln Gly
455                 460                 465                 470
ata gag acc aat tac tac ctc aat caa aat tct tac aac caa atc caa     1842
Ile Glu Thr Asn Tyr Tyr Leu Asn Gln Asn Ser Tyr Asn Gln Ile Gln
                475                 480                 485
acc atc aac caa gaa cta ggg cgt aac ccc ttt agg aaa gtg ggc atc     1890
Thr Ile Asn Gln Glu Leu Gly Arg Asn Pro Phe Arg Lys Val Gly Ile
            490                 495                 500
gtc aat tct caa acc aac aat ggt gcc atg aat ggg atc ggt att cag     1938
Val Asn Ser Gln Thr Asn Asn Gly Ala Met Asn Gly Ile Gly Ile Gln
        505                 510                 515
gtg ggc tat aag caa ttc ttt ggc caa aaa aga aaa tgg ggc gct agg     1986
Val Gly Tyr Lys Gln Phe Phe Gly Gln Lys Arg Lys Trp Gly Ala Arg
    520                 525                 530
tat tac ggc ttt ttt gac tac aac cat gcg ttc att aaa tcc agc ttc     2034
Tyr Tyr Gly Phe Phe Asp Tyr Asn His Ala Phe Ile Lys Ser Ser Phe
535                 540                 545                 550
ttc aac tcg gct tct gat gtg tgg act tat ggt ttt gga gcg gac gct     2082
Phe Asn Ser Ala Ser Asp Val Trp Thr Tyr Gly Phe Gly Ala Asp Ala
                555                 560                 565
ctt tat aac ttc atc aac gat aaa gcc acc aat ttc tta ggc aaa aac     2130
Leu Tyr Asn Phe Ile Asn Asp Lys Ala Thr Asn Phe Leu Gly Lys Asn
            570                 575                 580
aac aag ctt tcc gtg ggg ctt ttt gga ggg att gcg tta gcg ggc act     2178
Asn Lys Leu Ser Val Gly Leu Phe Gly Gly Ile Ala Leu Ala Gly Thr
        585                 590                 595
tca tgg ctt aat tct gag tat gtg aat tta gcc acc gtg aat aac gtc     2226
Ser Trp Leu Asn Ser Glu Tyr Val Asn Leu Ala Thr Val Asn Asn Val
    600                 605                 610
tat aac gct aaa atg aat gtg gcg aat ttc caa ttc tta ttc aat atg     2274
Tyr Asn Ala Lys Met Asn Val Ala Asn Phe Gln Phe Leu Phe Asn Met
615                 620                 625                 630
gga gtg agg atg aat tta gcc aga tcc aag aaa aaa ggc agc gat cat     2322
Gly Val Arg Met Asn Leu Ala Arg Ser Lys Lys Lys Gly Ser Asp His
                635                 640                 645
gcg gct cag cat ggg att gaa cta ggg ctt aaa atc ccc acc atc aac     2370
Ala Ala Gln His Gly Ile Glu Leu Gly Leu Lys Ile Pro Thr Ile Asn
            650                 655                 660
acg aac tat tat tct ttc atg ggg gct gaa ctc aaa tac aga agg ctt     2418
Thr Asn Tyr Tyr Ser Phe Met Gly Ala Glu Leu Lys Tyr Arg Arg Leu
        665                 670                 675
tat agc gtg tat ttg aat tat gtg ttc gct tac taagcttttt gtgaaactcc   2471
Tyr Ser Val Tyr Leu Asn Tyr Val Phe Ala Tyr
    680                 685
ctttttaagg ggtttttttt tgaactctct ttttaaattc tctttttaaa gagatttctt   2531
ttttttaagc ttttttttga attctttttt ttgaattctt tgtttttaag ctttttttaa   2591
accctttcgt ttttaaactc ccttttttaa gggatttctt tttttaaact cttttttttt   2651
aaactctttt ttttaaaccc tcttttttta agggatttct ttttaaagct tttttgaagt   2711
ctttttttaa attctttttt tgggggtttg atctttcttt ttgccaatcc ccactacttt   2771
cgctttttaa tctttaggtt ttatttt                                       2798
 
           
             2 
             708 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(19) 
             
           
            2
Met Lys Lys Thr Leu Leu Leu Ser Leu Ser Leu Ser Leu Ser Phe Leu
                -15                 -10                 -5
Leu His Ala Glu Asp Asp Gly Phe Tyr Thr Ser Val Gly Tyr Gln Ile
             1               5                  10
Gly Glu Ala Ala Gln Met Val Lys Asn Thr Lys Gly Ile Gln Glu Leu
    15                  20                  25
Ser Asp Asn Tyr Glu Lys Leu Asn Asn Leu Leu Asn Asn Tyr Ser Thr
30                  35                  40                  45
Leu Asn Thr Leu Ile Lys Leu Ser Ala Asp Pro Ser Ala Ile Asn Asp
                50                  55                  60
Ala Arg Asp Asn Leu Gly Ser Ser Ser Arg Asn Leu Leu Asp Val Lys
            65                  70                  75
Thr Asn Ser Pro Ala Tyr Gln Ala Val Leu Leu Ala Leu Asn Ala Ala
        80                  85                  90
Val Gly Leu Trp Gln Val Thr Ser Tyr Ala Phe Thr Ala Cys Gly Pro
    95                  100                 105
Gly Ser Asn Glu Asn Ala Asn Gly Gly Ile Gln Thr Phe Asn Asn Val
110                 115                 120                 125
Pro Gly Gln Asp Thr Thr Thr Ile Thr Cys Asn Ser Tyr Tyr Glu Pro
                130                 135                 140
Gly His Gly Gly Pro Ile Ser Thr Ala Asn Tyr Ala Lys Ile Asn Gln
            145                 150                 155
Ala Tyr Gln Ile Ile Gln Lys Ala Leu Thr Ala Asn Gly Ala Asn Gly
        160                 165                 170
Asp Gly Val Pro Val Leu Ser Asn Thr Thr Thr Lys Leu Asp Phe Thr
    175                 180                 185
Ile Asn Gly Asp Lys Arg Thr Gly Gly Lys Pro Asn Thr Pro Glu Lys
190                 195                 200                 205
Phe Pro Trp Ser Asp Gly Lys Tyr Ile His Thr Gln Trp Ile Asn Thr
                210                 215                 220
Ile Val Thr Pro Thr Glu Thr Asn Ile Asn Thr Glu Asn Asn Ala Gln
            225                 230                 235
Glu Leu Leu Lys Gln Ala Ser Ile Ile Ile Thr Thr Leu Asn Glu Ala
        240                 245                 250
Cys Pro Asn Phe Gln Asn Gly Gly Arg Ser Tyr Trp Gln Gly Ile Ser
    255                 260                 265
Gly Asn Gly Thr Met Cys Gly Met Phe Lys Asn Glu Ile Ser Ala Ile
270                 275                 280                 285
Gln Gly Met Ile Ala Asn Ala Gln Glu Ala Val Ala Gln Ser Lys Ile
                290                 295                 300
Val Ser Glu Asn Ala Gln Asn Gln Asn Asn Leu Asp Thr Gly Lys Pro
            305                 310                 315
Phe Asn Pro Tyr Thr Asp Ala Ser Phe Ala Gln Ser Met Leu Lys Asn
        320                 325                 330
Ala Gln Ala Gln Ala Glu Ile Leu Asn Gln Ala Glu Gln Val Val Lys
    335                 340                 345
Asn Phe Glu Lys Ile Pro Thr Ala Phe Val Ser Asp Ser Leu Gly Val
350                 355                 360                 365
Cys Tyr Glu Val Gln Gly Gly Glu Arg Arg Gly Thr Asn Pro Gly Gln
                370                 375                 380
Val Thr Ser Asn Thr Trp Gly Ala Gly Cys Ala Tyr Val Lys Gln Thr
            385                 390                 395
Ile Thr Asn Leu Asp Asn Ser Ile Ala His Phe Gly Thr Gln Glu Gln
        400                 405                 410
Gln Ile Gln Gln Ala Glu Asn Ile Ala Asp Thr Leu Val Asn Phe Lys
    415                 420                 425
Ser Arg Tyr Ser Glu Leu Gly Asn Thr Tyr Asn Ser Ile Thr Thr Ala
430                 435                 440                 445
Leu Ser Lys Val Pro Asn Ala Gln Ser Leu Gln Asn Val Val Ser Lys
                450                 455                 460
Lys Asn Asn Pro Tyr Ser Pro Gln Gly Ile Glu Thr Asn Tyr Tyr Leu
            465                 470                 475
Asn Gln Asn Ser Tyr Asn Gln Ile Gln Thr Ile Asn Gln Glu Leu Gly
        480                 485                 490
Arg Asn Pro Phe Arg Lys Val Gly Ile Val Asn Ser Gln Thr Asn Asn
    495                 500                 505
Gly Ala Met Asn Gly Ile Gly Ile Gln Val Gly Tyr Lys Gln Phe Phe
510                 515                 520                 525
Gly Gln Lys Arg Lys Trp Gly Ala Arg Tyr Tyr Gly Phe Phe Asp Tyr
                530                 535                 540
Asn His Ala Phe Ile Lys Ser Ser Phe Phe Asn Ser Ala Ser Asp Val
            545                 550                 555
Trp Thr Tyr Gly Phe Gly Ala Asp Ala Leu Tyr Asn Phe Ile Asn Asp
        560                 565                 570
Lys Ala Thr Asn Phe Leu Gly Lys Asn Asn Lys Leu Ser Val Gly Leu
    575                 580                 585
Phe Gly Gly Ile Ala Leu Ala Gly Thr Ser Trp Leu Asn Ser Glu Tyr
590                 595                 600                 605
Val Asn Leu Ala Thr Val Asn Asn Val Tyr Asn Ala Lys Met Asn Val
                610                 615                 620
Ala Asn Phe Gln Phe Leu Phe Asn Met Gly Val Arg Met Asn Leu Ala
            625                 630                 635
Arg Ser Lys Lys Lys Gly Ser Asp His Ala Ala Gln His Gly Ile Glu
        640                 645                 650
Leu Gly Leu Lys Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Phe Met
    655                 660                 665
Gly Ala Glu Leu Lys Tyr Arg Arg Leu Tyr Ser Val Tyr Leu Asn Tyr
670                 675                 680                 685
Val Phe Ala Tyr
 
           
             3 
             2699 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (199)...(2397) 
             
             
               sig_peptide 
               (199)...(259) 
             
           
            3
taaaatccaa ttaaaagcgt tcaaaggtaa cgcaaaaaaa caaaaaatga cgcaattttt     60
tcaaaatgac aaaaaaaaac gctttatgct ataatacccc aaatacattc taatagcaaa    120
tgcgttctaa tgcaaatgca ttccaatgta tgaaatccct aatactaaat ccaatttaat    180
ccaaaaagga gaaaaaac atg aaa aaa cac atc ctt tca tta gct tta ggc      231
                    Met Lys Lys His Ile Leu Ser Leu Ala Leu Gly
                    -20                 -15                 -10
tcg ctt tta gtt tcc act ttg agc gct gaa gac gac ggc ttt tac aca      279
Ser Leu Leu Val Ser Thr Leu Ser Ala Glu Asp Asp Gly Phe Tyr Thr
                 -5                 1               5
agc gta ggc tat cag atc ggt gaa gcc gct caa atg gta aca aac acc      327
Ser Val Gly Tyr Gln Ile Gly Glu Ala Ala Gln Met Val Thr Asn Thr
         10                  15                  20
aaa ggc atc caa cag ctt tca gac aat tat gaa aat ttg aac aac ctt      375
Lys Gly Ile Gln Gln Leu Ser Asp Asn Tyr Glu Asn Leu Asn Asn Leu
     25                  30                  35
tta acg aga tac agc acc cta aac acc ctt atc aaa ttg tcc gct gat      423
Leu Thr Arg Tyr Ser Thr Leu Asn Thr Leu Ile Lys Leu Ser Ala Asp
 40                  45                  50                  55
ccg agc gca att aat gcg gtg cgg gaa aat ctg ggc gcg agc gcg aag      471
Pro Ser Ala Ile Asn Ala Val Arg Glu Asn Leu Gly Ala Ser Ala Lys
                 60                  65                  70
aat ttg atc ggc gat aaa gcc aac tcc ccc gcc tat caa gcc gtg ctt      519
Asn Leu Ile Gly Asp Lys Ala Asn Ser Pro Ala Tyr Gln Ala Val Leu
             75                  80                  85
tta gcg atc aac gcg gcg gta ggg ttt tgg aat gtc gtg ggc tat gtg      567
Leu Ala Ile Asn Ala Ala Val Gly Phe Trp Asn Val Val Gly Tyr Val
         90                  95                 100
acg caa tgt ggg ggt aac gcc aat ggt caa gaa agc acc tct tca acc      615
Thr Gln Cys Gly Gly Asn Ala Asn Gly Gln Glu Ser Thr Ser Ser Thr
    105                 110                 115
acc atc ttc aac aac gag cca ggg tat cga tcc act tcc atc act tgt      663
Thr Ile Phe Asn Asn Glu Pro Gly Tyr Arg Ser Thr Ser Ile Thr Cys
120                 125                 130                 135
tct ttg aac ggg cat aag cct gga tac tat ggc cct atg agc att gag      711
Ser Leu Asn Gly His Lys Pro Gly Tyr Tyr Gly Pro Met Ser Ile Glu
                140                 145                 150
aat ttt aaa aag ctt aac gaa gcc tat cag atc ctc caa acg gct tta      759
Asn Phe Lys Lys Leu Asn Glu Ala Tyr Gln Ile Leu Gln Thr Ala Leu
            155                 160                 165
aaa aac ggc tta ccc gcg ctc aaa gaa aac aac ggg aag gtc agt gta      807
Lys Asn Gly Leu Pro Ala Leu Lys Glu Asn Asn Gly Lys Val Ser Val
        170                 175                 180
acc tat acc tac aca tgc tca ggg caa ggg aat aat aac tgc tcg cca      855
Thr Tyr Thr Tyr Thr Cys Ser Gly Gln Gly Asn Asn Asn Cys Ser Pro
    185                 190                 195
agt gtc aac gga acc aaa acc aca acc caa acc ata gac ggc aaa agc      903
Ser Val Asn Gly Thr Lys Thr Thr Thr Gln Thr Ile Asp Gly Lys Ser
200                 205                 210                 215
gta acc acc acg atc agt tca aaa gtg gtt ggt agc atc gct agt ggc      951
Val Thr Thr Thr Ile Ser Ser Lys Val Val Gly Ser Ile Ala Ser Gly
                220                 225                 230
aac aca tca cat gtc atc acc aac aaa tta gac ggt gtg cct gat agc      999
Asn Thr Ser His Val Ile Thr Asn Lys Leu Asp Gly Val Pro Asp Ser
            235                 240                 245
gct caa gcg ctc tta gcg caa gcg agc acg ctc atc aac acc atc aac     1047
Ala Gln Ala Leu Leu Ala Gln Ala Ser Thr Leu Ile Asn Thr Ile Asn
        250                 255                 260
gaa gca tgc ccg tat ttc cat gct act aat agt agt gag gct aac gcc     1095
Glu Ala Cys Pro Tyr Phe His Ala Thr Asn Ser Ser Glu Ala Asn Ala
    265                 270                 275
cca aaa ttc tct act act act ggg aaa ata tgc ggc gct ttt tca gaa     1143
Pro Lys Phe Ser Thr Thr Thr Gly Lys Ile Cys Gly Ala Phe Ser Glu
280                 285                 290                 295
gaa atc agc gcg atc caa aag atg atc acg gac gcg caa gag cta gtt     1191
Glu Ile Ser Ala Ile Gln Lys Met Ile Thr Asp Ala Gln Glu Leu Val
                300                 305                 310
aat caa acg agc gtc att aac agc aac gaa caa tca act ccg gta ggc     1239
Asn Gln Thr Ser Val Ile Asn Ser Asn Glu Gln Ser Thr Pro Val Gly
            315                 320                 325
aat aat aat ggc aag cct ttc aac cct ttc acg gac gca agt ttt gcg     1287
Asn Asn Asn Gly Lys Pro Phe Asn Pro Phe Thr Asp Ala Ser Phe Ala
        330                 335                 340
caa ggc atg ctc gct aac gct agc gcg caa gct aaa atg ctc aat tta     1335
Gln Gly Met Leu Ala Asn Ala Ser Ala Gln Ala Lys Met Leu Asn Leu
    345                 350                 355
gcc cat cag gtg ggg caa gcc att aac cca gag aat ctt agc gag aat     1383
Ala His Gln Val Gly Gln Ala Ile Asn Pro Glu Asn Leu Ser Glu Asn
360                 365                 370                 375
ttt aaa aat ttt gtt aca ggc ttt tta gcc aca tgc aat aac aaa tca     1431
Phe Lys Asn Phe Val Thr Gly Phe Leu Ala Thr Cys Asn Asn Lys Ser
                380                 385                 390
aca gct ggc act ggt ggc aca caa ggt tca gct cca ggc aca gtg acc     1479
Thr Ala Gly Thr Gly Gly Thr Gln Gly Ser Ala Pro Gly Thr Val Thr
            395                 400                 405
act caa act ttc gct tct ggt tgc gcg tat gtg gag caa acc cta acg     1527
Thr Gln Thr Phe Ala Ser Gly Cys Ala Tyr Val Glu Gln Thr Leu Thr
        410                 415                 420
aac tta ggc aac agc atc gct cac ttt ggc act caa gag cag cag ata     1575
Asn Leu Gly Asn Ser Ile Ala His Phe Gly Thr Gln Glu Gln Gln Ile
    425                 430                 435
cag caa gcc gaa aac atc gct gac act cta gtg aat ttc aaa tct aga     1623
Gln Gln Ala Glu Asn Ile Ala Asp Thr Leu Val Asn Phe Lys Ser Arg
440                 445                 450                 455
tac agc gaa tta ggc aac acc tat aac agc atc acc acc gcg ctc tcc     1671
Tyr Ser Glu Leu Gly Asn Thr Tyr Asn Ser Ile Thr Thr Ala Leu Ser
                460                 465                 470
aaa gtc cct aac gcg caa agc ttg caa aac gtg gtg agc aaa aag aat     1719
Lys Val Pro Asn Ala Gln Ser Leu Gln Asn Val Val Ser Lys Lys Asn
            475                 480                 485
aac ccc tat agc cct caa ggc ata gag acc aat tac tac ctc aat caa     1767
Asn Pro Tyr Ser Pro Gln Gly Ile Glu Thr Asn Tyr Tyr Leu Asn Gln
        490                 495                 500
aat tct tac aac caa atc caa acc atc aac caa gaa cta ggg cgt aac     1815
Asn Ser Tyr Asn Gln Ile Gln Thr Ile Asn Gln Glu Leu Gly Arg Asn
    505                 510                 515
ccc ttt agg aaa gtg ggc atc gtc aat tct caa acc aac aat ggt gcc     1863
Pro Phe Arg Lys Val Gly Ile Val Asn Ser Gln Thr Asn Asn Gly Ala
520                 525                 530                 535
atg aat ggg atc ggt att cag gtg ggc tat aag caa ttc ttt ggc caa     1911
Met Asn Gly Ile Gly Ile Gln Val Gly Tyr Lys Gln Phe Phe Gly Gln
                540                 545                 550
aaa aga aaa tgg ggc gct agg tat tac ggc ttt ttt gat tac aac cat     1959
Lys Arg Lys Trp Gly Ala Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn His
            555                 560                 565
gcg ttc atc aaa tcc agc ttt ttc aac tcg gct tct gac gtg tgg act     2007
Ala Phe Ile Lys Ser Ser Phe Phe Asn Ser Ala Ser Asp Val Trp Thr
        570                 575                 580
tat ggt ttt gga gcg gac gcg ctt tat aac ttc atc aac gat aaa gcc     2055
Tyr Gly Phe Gly Ala Asp Ala Leu Tyr Asn Phe Ile Asn Asp Lys Ala
    585                 590                 595
acc aat ttc tta ggc aaa aac aac aag ctt tct ttg ggg ctt ttt ggc     2103
Thr Asn Phe Leu Gly Lys Asn Asn Lys Leu Ser Leu Gly Leu Phe Gly
600                 605                 610                 615
ggg att gcg tta gcg ggc act tca tgg ctc aat tct gag tac gtg aat     2151
Gly Ile Ala Leu Ala Gly Thr Ser Trp Leu Asn Ser Glu Tyr Val Asn
                620                 625                 630
tta gcc acc gtg aat aac gtc tat aac gct aaa atg aat gtg gcg aat     2199
Leu Ala Thr Val Asn Asn Val Tyr Asn Ala Lys Met Asn Val Ala Asn
            635                 640                 645
ttc caa ttc tta ttc aat atg gga gtg agg atg aat tta gcc aga tcc     2247
Phe Gln Phe Leu Phe Asn Met Gly Val Arg Met Asn Leu Ala Arg Ser
        650                 655                 660
aag aaa aaa ggc agc gat cat gca gct cag cat ggg att gag tta ggg     2295
Lys Lys Lys Gly Ser Asp His Ala Ala Gln His Gly Ile Glu Leu Gly
    665                 670                 675
ctt aaa atc ccc acc atc aac acg aac tat tat tcc ttt atg ggg gct     2343
Leu Lys Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Phe Met Gly Ala
680                 685                 690                 695
gaa ctc aaa tac aga agg ctc tat agc gtg tat ttg aac tat gtg ttc     2391
Glu Leu Lys Tyr Arg Arg Leu Tyr Ser Val Tyr Leu Asn Tyr Val Phe
                700                 705                 710
gct tac taatgtttgg ctctttgtga aactcccttt ttaaggggtt tttttttgaa      2447
Ala Tyr
ctctcttttt aaattctctt tttaaagaga tttctttttt ttaagctttt ttttgaattc   2507
tttttttttg aattctttgt ttttaagctt tttttaaacc ctttcgtttt taaactccct   2567
tttttaaggg atttcttttt ttgaactccc ttttttgaac cctttttttt aaaccctctt   2627
tttttaaggg gtttcttttt aaagcttttt tgaagtcttt ttttaaattc tttttttggg   2687
ggtttgatct tt                                                       2699
 
           
             4 
             733 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(20) 
             
           
            4
Met Lys Lys His Ile Leu Ser Leu Ala Leu Gly Ser Leu Leu Val Ser
-20                 -15                 -10                 -5
Thr Leu Ser Ala Glu Asp Asp Gly Phe Tyr Thr Ser Val Gly Tyr Gln
                 1               5                  10
Ile Gly Glu Ala Ala Gln Met Val Thr Asn Thr Lys Gly Ile Gln Gln
        15                  20                  25
Leu Ser Asp Asn Tyr Glu Asn Leu Asn Asn Leu Leu Thr Arg Tyr Ser
    30                  35                  40
Thr Leu Asn Thr Leu Ile Lys Leu Ser Ala Asp Pro Ser Ala Ile Asn
45                  50                  55                  60
Ala Val Arg Glu Asn Leu Gly Ala Ser Ala Lys Asn Leu Ile Gly Asp
                65                  70                  75
Lys Ala Asn Ser Pro Ala Tyr Gln Ala Val Leu Leu Ala Ile Asn Ala
            80                  85                  90
Ala Val Gly Phe Trp Asn Val Val Gly Tyr Val Thr Gln Cys Gly Gly
        95                  100                 105
Asn Ala Asn Gly Gln Glu Ser Thr Ser Ser Thr Thr Ile Phe Asn Asn
    110                 115                 120
Glu Pro Gly Tyr Arg Ser Thr Ser Ile Thr Cys Ser Leu Asn Gly His
125                 130                 135                 140
Lys Pro Gly Tyr Tyr Gly Pro Met Ser Ile Glu Asn Phe Lys Lys Leu
                145                 150                 155
Asn Glu Ala Tyr Gln Ile Leu Gln Thr Ala Leu Lys Asn Gly Leu Pro
            160                 165                 170
Ala Leu Lys Glu Asn Asn Gly Lys Val Ser Val Thr Tyr Thr Tyr Thr
        175                 180                 185
Cys Ser Gly Gln Gly Asn Asn Asn Cys Ser Pro Ser Val Asn Gly Thr
    190                 195                 200
Lys Thr Thr Thr Gln Thr Ile Asp Gly Lys Ser Val Thr Thr Thr Ile
205                 210                 215                 220
Ser Ser Lys Val Val Gly Ser Ile Ala Ser Gly Asn Thr Ser His Val
                225                 230                 235
Ile Thr Asn Lys Leu Asp Gly Val Pro Asp Ser Ala Gln Ala Leu Leu
            240                 245                 250
Ala Gln Ala Ser Thr Leu Ile Asn Thr Ile Asn Glu Ala Cys Pro Tyr
        255                 260                 265
Phe His Ala Thr Asn Ser Ser Glu Ala Asn Ala Pro Lys Phe Ser Thr
    270                 275                 280
Thr Thr Gly Lys Ile Cys Gly Ala Phe Ser Glu Glu Ile Ser Ala Ile
285                 290                 295                 300
Gln Lys Met Ile Thr Asp Ala Gln Glu Leu Val Asn Gln Thr Ser Val
                305                 310                 315
Ile Asn Ser Asn Glu Gln Ser Thr Pro Val Gly Asn Asn Asn Gly Lys
            320                 325                 330
Pro Phe Asn Pro Phe Thr Asp Ala Ser Phe Ala Gln Gly Met Leu Ala
        335                 340                 345
Asn Ala Ser Ala Gln Ala Lys Met Leu Asn Leu Ala His Gln Val Gly
    350                 355                 360
Gln Ala Ile Asn Pro Glu Asn Leu Ser Glu Asn Phe Lys Asn Phe Val
365                 370                 375                 380
Thr Gly Phe Leu Ala Thr Cys Asn Asn Lys Ser Thr Ala Gly Thr Gly
                385                 390                 395
Gly Thr Gln Gly Ser Ala Pro Gly Thr Val Thr Thr Gln Thr Phe Ala
            400                 405                 410
Ser Gly Cys Ala Tyr Val Glu Gln Thr Leu Thr Asn Leu Gly Asn Ser
        415                 420                 425
Ile Ala His Phe Gly Thr Gln Glu Gln Gln Ile Gln Gln Ala Glu Asn
    430                 435                 440
Ile Ala Asp Thr Leu Val Asn Phe Lys Ser Arg Tyr Ser Glu Leu Gly
445                 450                 455                 460
Asn Thr Tyr Asn Ser Ile Thr Thr Ala Leu Ser Lys Val Pro Asn Ala
                465                 470                 475
Gln Ser Leu Gln Asn Val Val Ser Lys Lys Asn Asn Pro Tyr Ser Pro
            480                 485                 490
Gln Gly Ile Glu Thr Asn Tyr Tyr Leu Asn Gln Asn Ser Tyr Asn Gln
        495                 500                 505
Ile Gln Thr Ile Asn Gln Glu Leu Gly Arg Asn Pro Phe Arg Lys Val
    510                 515                 520
Gly Ile Val Asn Ser Gln Thr Asn Asn Gly Ala Met Asn Gly Ile Gly
525                 530                 535                 540
Ile Gln Val Gly Tyr Lys Gln Phe Phe Gly Gln Lys Arg Lys Trp Gly
                545                 550                 555
Ala Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn His Ala Phe Ile Lys Ser
            560                 565                 570
Ser Phe Phe Asn Ser Ala Ser Asp Val Trp Thr Tyr Gly Phe Gly Ala
        575                 580                 585
Asp Ala Leu Tyr Asn Phe Ile Asn Asp Lys Ala Thr Asn Phe Leu Gly
    590                 595                 600
Lys Asn Asn Lys Leu Ser Leu Gly Leu Phe Gly Gly Ile Ala Leu Ala
605                 610                 615                 620
Gly Thr Ser Trp Leu Asn Ser Glu Tyr Val Asn Leu Ala Thr Val Asn
                625                 630                 635
Asn Val Tyr Asn Ala Lys Met Asn Val Ala Asn Phe Gln Phe Leu Phe
            640                 645                 650
Asn Met Gly Val Arg Met Asn Leu Ala Arg Ser Lys Lys Lys Gly Ser
        655                 660                 665
Asp His Ala Ala Gln His Gly Ile Glu Leu Gly Leu Lys Ile Pro Thr
    670                 675                 680
Ile Asn Thr Asn Tyr Tyr Ser Phe Met Gly Ala Glu Leu Lys Tyr Arg
685                 690                 695                 700
Arg Leu Tyr Ser Val Tyr Leu Asn Tyr Val Phe Ala Tyr
                705                 710
 
           
             5 
             2915 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (365)...(2599) 
             
             
               sig_peptide 
               (365)...(425) 
             
             
               misc_feature 
               2585 
               n = A,T,C or G 
             
           
            5
ttttaggcga caaaatcgct tatgttgggg ataaaggcaa cccgcacaat ttcgctcaca     60
agaaataaac cgctcataag gggcaaacgc cccaaaaaag cgatttttaa agaggttacg    120
gcaaaatcaa gctctttagt atttaatctt aaaaaatgct aaaagccttt ttatgggcta    180
acaccacaca aaaagcatca aaatcaaaaa aatgacaaaa tttttaagaa aatgacaaaa    240
aaaaacgctt tatgctataa taccccaaat acattctaat agcaaatgcg ttctaatgca    300
aatgcattcc aatgtatgaa atccctaata ctaaatccaa tttaatccaa aaaggagaaa    360
aaac atg aaa aaa cac atc ctt tca tta gct tta ggc tcg ctt tta gtt     409
     Met Lys Lys His Ile Leu Ser Leu Ala Leu Gly Ser Leu Leu Val
     -20                 -15                 -10
tcc act ttg agc gct gaa gac gac ggc ttt tac aca agc gta ggc tat      457
Ser Thr Leu Ser Ala Glu Asp Asp Gly Phe Tyr Thr Ser Val Gly Tyr
 -5                 1               5                   10
cag atc ggt gaa gcc gct caa atg gta aca aac acc aaa ggc atc caa      505
Gln Ile Gly Glu Ala Ala Gln Met Val Thr Asn Thr Lys Gly Ile Gln
             15                  20                  25
cag ctt tca gac aat tat gaa aat ttg aac aac ctt tta acg aga tac      553
Gln Leu Ser Asp Asn Tyr Glu Asn Leu Asn Asn Leu Leu Thr Arg Tyr
         30                  35                  40
agc acc cta aac acc ctt atc aaa ttg tcc gct gat ccg agc gca att      601
Ser Thr Leu Asn Thr Leu Ile Lys Leu Ser Ala Asp Pro Ser Ala Ile
     45                  50                  55
aat gcg gtg cgg gaa aat ctg ggc gcg agc acg aag aat ttg atc ggc      649
Asn Ala Val Arg Glu Asn Leu Gly Ala Ser Thr Lys Asn Leu Ile Gly
 60                  65                  70                  75
gat aaa gcc aac tcc ccg gcg tat caa gcc gtg ttt tta gcg atc aac      697
Asp Lys Ala Asn Ser Pro Ala Tyr Gln Ala Val Phe Leu Ala Ile Asn
                 80                  85                  90
gcg gcg gta ggg ttg tgg aat acc atc ggc tat gcg gtc atg tgc ggg      745
Ala Ala Val Gly Leu Trp Asn Thr Ile Gly Tyr Ala Val Met Cys Gly
             95                 100                 105
aac ggg aac ggc aca gag agt ggg cct ggc agc gtg atc ttt aat gac      793
Asn Gly Asn Gly Thr Glu Ser Gly Pro Gly Ser Val Ile Phe Asn Asp
        110                 115                 120
caa cca gga cag gat tcc acg caa att act tgc aac cgc ttt gaa tca      841
Gln Pro Gly Gln Asp Ser Thr Gln Ile Thr Cys Asn Arg Phe Glu Ser
    125                 130                 135
act ggg cct ggt aaa agc atg tct att gat gaa ttc aaa aaa ctc aat      889
Thr Gly Pro Gly Lys Ser Met Ser Ile Asp Glu Phe Lys Lys Leu Asn
140                 145                 150                 155
gaa gcc tat caa atc atc cag caa gct tta aaa aat caa agt ggg ttt      937
Glu Ala Tyr Gln Ile Ile Gln Gln Ala Leu Lys Asn Gln Ser Gly Phe
                160                 165                 170
cct gaa tta ggc ggg aac ggc aca aaa gtg agt gtt aat tac aat tac      985
Pro Glu Leu Gly Gly Asn Gly Thr Lys Val Ser Val Asn Tyr Asn Tyr
            175                 180                 185
gaa tgc aga caa act gct gat atc aac ggc ggt gtg tat cag ttc tgc     1033
Glu Cys Arg Gln Thr Ala Asp Ile Asn Gly Gly Val Tyr Gln Phe Cys
        190                 195                 200
aag gct aaa aat ggt agt agt agc agt agt aat ggc ggt aat ggc agt     1081
Lys Ala Lys Asn Gly Ser Ser Ser Ser Ser Asn Gly Gly Asn Gly Ser
    205                 210                 215
agc acg caa aca acc gcg aca acc acg caa gac ggc gta acg atc acc     1129
Ser Thr Gln Thr Thr Ala Thr Thr Thr Gln Asp Gly Val Thr Ile Thr
220                 225                 230                 235
act acc tat aat aat aac aaa gcc acc gtc aaa ttt gac atc acc aat     1177
Thr Thr Tyr Asn Asn Asn Lys Ala Thr Val Lys Phe Asp Ile Thr Asn
                240                 245                 250
aac gct gaa cag ctg tta aat caa gcg gca aac atc atg caa gtc ctt     1225
Asn Ala Glu Gln Leu Leu Asn Gln Ala Ala Asn Ile Met Gln Val Leu
            255                 260                 265
aat acg caa tgc cct tta gtg cgt tcc acg aat aac gaa aac act cca     1273
Asn Thr Gln Cys Pro Leu Val Arg Ser Thr Asn Asn Glu Asn Thr Pro
        270                 275                 280
ggg ggt ggt caa cca tgg ggt tta agc aca tcc ggg aat gcg tgc agc     1321
Gly Gly Gly Gln Pro Trp Gly Leu Ser Thr Ser Gly Asn Ala Cys Ser
    285                 290                 295
atc ttc caa caa gaa ttt agc cag gtt act agc atg atc aaa aac gcc     1369
Ile Phe Gln Gln Glu Phe Ser Gln Val Thr Ser Met Ile Lys Asn Ala
300                 305                 310                 315
caa gaa ata atc gcg caa agc aaa atc gtt agt gaa aac gcg caa aat     1417
Gln Glu Ile Ile Ala Gln Ser Lys Ile Val Ser Glu Asn Ala Gln Asn
                320                 325                 330
caa aac aac ttg gat act gga aaa cca ttc aac cct tac acg gac gcc     1465
Gln Asn Asn Leu Asp Thr Gly Lys Pro Phe Asn Pro Tyr Thr Asp Ala
            335                 340                 345
agc ttt gcg caa agc atg ctc aaa aac gct caa gcg caa gca gag atg     1513
Ser Phe Ala Gln Ser Met Leu Lys Asn Ala Gln Ala Gln Ala Glu Met
        350                 355                 360
ttc aat ttg agc gaa caa gtg aaa aag aac ttg gaa gtc atg aaa aac     1561
Phe Asn Leu Ser Glu Gln Val Lys Lys Asn Leu Glu Val Met Lys Asn
    365                 370                 375
aac aat aat gtt aac gag aaa tta gca gga ttt ggg aaa gaa gaa gta     1609
Asn Asn Asn Val Asn Glu Lys Leu Ala Gly Phe Gly Lys Glu Glu Val
380                 385                 390                 395
atg acc aat ttt gtt agc gcc ttt ttg gca agc tgc aaa gat ggt ggc     1657
Met Thr Asn Phe Val Ser Ala Phe Leu Ala Ser Cys Lys Asp Gly Gly
                400                 405                 410
aca ttg cct aat gca ggg gtt act tct aac act tgg ggg gcg ggt tgc     1705
Thr Leu Pro Asn Ala Gly Val Thr Ser Asn Thr Trp Gly Ala Gly Cys
            415                 420                 425
gcg tat gtg gga gag acg ata agc gcc cta acc aac agc atc gct cac     1753
Ala Tyr Val Gly Glu Thr Ile Ser Ala Leu Thr Asn Ser Ile Ala His
        430                 435                 440
ttt ggc act caa gag cag cag ata cag caa gcc gaa aac atc gct gac     1801
Phe Gly Thr Gln Glu Gln Gln Ile Gln Gln Ala Glu Asn Ile Ala Asp
    445                 450                 455
act cta gtg aat ttc aaa tct aga tac agc gaa tta ggc aac acc tat     1849
Thr Leu Val Asn Phe Lys Ser Arg Tyr Ser Glu Leu Gly Asn Thr Tyr
460                 465                 470                 475
aac agc atc acc acc gcg ctc tcc aaa gtc cct aac gcg caa agc ttg     1897
Asn Ser Ile Thr Thr Ala Leu Ser Lys Val Pro Asn Ala Gln Ser Leu
                480                 485                 490
caa aac gtg gtg agc aaa aag aat aac ccc tat agc cct caa ggc ata     1945
Gln Asn Val Val Ser Lys Lys Asn Asn Pro Tyr Ser Pro Gln Gly Ile
            495                 500                 505
gag acc aat tac tac ctc aat caa aat tct tac aac caa atc caa acc     1993
Glu Thr Asn Tyr Tyr Leu Asn Gln Asn Ser Tyr Asn Gln Ile Gln Thr
        510                 515                 520
atc aac caa gaa cta ggg cgt aac ccc ttt agg aaa gtg ggc atc gtc     2041
Ile Asn Gln Glu Leu Gly Arg Asn Pro Phe Arg Lys Val Gly Ile Val
    525                 530                 535
aat tct caa acc aac aat ggt gcc atg aat ggg atc ggc att cag gtg     2089
Asn Ser Gln Thr Asn Asn Gly Ala Met Asn Gly Ile Gly Ile Gln Val
540                 545                 550                 555
ggc tat aag caa ttc ttt ggc caa aaa aga aaa tgg ggc gct agg tat     2137
Gly Tyr Lys Gln Phe Phe Gly Gln Lys Arg Lys Trp Gly Ala Arg Tyr
                560                 565                 570
tac ggc ttt ttt gat tac aac cat gcg ttc atc aaa tcc agc ttt ttc     2185
Tyr Gly Phe Phe Asp Tyr Asn His Ala Phe Ile Lys Ser Ser Phe Phe
            575                 580                 585
aac tcg gct tct gac gtg tgg act tat ggt ttt gga gcg gac gcg ctt     2233
Asn Ser Ala Ser Asp Val Trp Thr Tyr Gly Phe Gly Ala Asp Ala Leu
        590                 595                 600
tat aac ttc atc aac gat aaa gcc acc aat ttc tta ggc aaa aac aac     2281
Tyr Asn Phe Ile Asn Asp Lys Ala Thr Asn Phe Leu Gly Lys Asn Asn
    605                 610                 615
aag ctt tct ttg ggg ctt ttt ggc ggg att gcg tta gcg ggc act tca     2329
Lys Leu Ser Leu Gly Leu Phe Gly Gly Ile Ala Leu Ala Gly Thr Ser
620                 625                 630                 635
tgg ctc aat tct gag tac gtg aat tta gcc acc gtg aat aac gtc tat     2377
Trp Leu Asn Ser Glu Tyr Val Asn Leu Ala Thr Val Asn Asn Val Tyr
                640                 645                 650
aac gct aaa atg aat gtg gcg aat ttc caa ttc tta ttc aat atg gga     2425
Asn Ala Lys Met Asn Val Ala Asn Phe Gln Phe Leu Phe Asn Met Gly
            655                 660                 665
gtg agg atg aat tta gcc aga tcc aag aaa aaa ggc agc gat cat gca     2473
Val Arg Met Asn Leu Ala Arg Ser Lys Lys Lys Gly Ser Asp His Ala
        670                 675                 680
gct cag cat ggg att gag tta ggg ctt aaa atc ccc acc atc aac acg     2521
Ala Gln His Gly Ile Glu Leu Gly Leu Lys Ile Pro Thr Ile Asn Thr
    685                 690                 695
aac tat tat tcc ttt atg ggg gct gaa ctc aaa tac aga agg ctc tat     2569
Asn Tyr Tyr Ser Phe Met Gly Ala Glu Leu Lys Tyr Arg Arg Leu Tyr
700                 705                 710                 715
agc gtg tat ttg aat nat gtg ttc gct tac taagcttttt gtgaaactcc       2619
Ser Val Tyr Leu Asn Xaa Val Phe Ala Tyr
                720                 725
ctttttaagg ggtttttttt tgaactctct tttaaattct ctttttaaag agatttcttt   2679
ttttaagctt ttttttgaac ttttttttga attctttgtt tttaagcttt ttttaaaccc   2739
tttcgttttt aaactccctt ttttaaggga tttctttttt tgaactccct tttttgaacc   2799
ctttttttta aaccctcttt ttttaagggg tttcttttta aagctttttt gaagtctttt   2859
tttaaattct ttttttgggg gtttgatctt tctttttgcc aatccccact actttc       2915
 
           
             6 
             745 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(20) 
             
             
               VARIANT 
               721 
               Xaa = Any Amino Acid 
             
           
            6
Met Lys Lys His Ile Leu Ser Leu Ala Leu Gly Ser Leu Leu Val Ser
-20                 -15                 -10                 -5
Thr Leu Ser Ala Glu Asp Asp Gly Phe Tyr Thr Ser Val Gly Tyr Gln
                 1               5                  10
Ile Gly Glu Ala Ala Gln Met Val Thr Asn Thr Lys Gly Ile Gln Gln
        15                  20                  25
Leu Ser Asp Asn Tyr Glu Asn Leu Asn Asn Leu Leu Thr Arg Tyr Ser
    30                  35                  40
Thr Leu Asn Thr Leu Ile Lys Leu Ser Ala Asp Pro Ser Ala Ile Asn
45                  50                  55                  60
Ala Val Arg Glu Asn Leu Gly Ala Ser Thr Lys Asn Leu Ile Gly Asp
                65                  70                  75
Lys Ala Asn Ser Pro Ala Tyr Gln Ala Val Phe Leu Ala Ile Asn Ala
            80                  85                  90
Ala Val Gly Leu Trp Asn Thr Ile Gly Tyr Ala Val Met Cys Gly Asn
        95                  100                 105
Gly Asn Gly Thr Glu Ser Gly Pro Gly Ser Val Ile Phe Asn Asp Gln
    110                 115                 120
Pro Gly Gln Asp Ser Thr Gln Ile Thr Cys Asn Arg Phe Glu Ser Thr
125                 130                 135                 140
Gly Pro Gly Lys Ser Met Ser Ile Asp Glu Phe Lys Lys Leu Asn Glu
                145                 150                 155
Ala Tyr Gln Ile Ile Gln Gln Ala Leu Lys Asn Gln Ser Gly Phe Pro
            160                 165                 170
Glu Leu Gly Gly Asn Gly Thr Lys Val Ser Val Asn Tyr Asn Tyr Glu
        175                 180                 185
Cys Arg Gln Thr Ala Asp Ile Asn Gly Gly Val Tyr Gln Phe Cys Lys
    190                 195                 200
Ala Lys Asn Gly Ser Ser Ser Ser Ser Asn Gly Gly Asn Gly Ser Ser
205                 210                 215                 220
Thr Gln Thr Thr Ala Thr Thr Thr Gln Asp Gly Val Thr Ile Thr Thr
                225                 230                 235
Thr Tyr Asn Asn Asn Lys Ala Thr Val Lys Phe Asp Ile Thr Asn Asn
            240                 245                 250
Ala Glu Gln Leu Leu Asn Gln Ala Ala Asn Ile Met Gln Val Leu Asn
        255                 260                 265
Thr Gln Cys Pro Leu Val Arg Ser Thr Asn Asn Glu Asn Thr Pro Gly
    270                 275                 280
Gly Gly Gln Pro Trp Gly Leu Ser Thr Ser Gly Asn Ala Cys Ser Ile
285                 290                 295                 300
Phe Gln Gln Glu Phe Ser Gln Val Thr Ser Met Ile Lys Asn Ala Gln
                305                 310                 315
Glu Ile Ile Ala Gln Ser Lys Ile Val Ser Glu Asn Ala Gln Asn Gln
            320                 325                 330
Asn Asn Leu Asp Thr Gly Lys Pro Phe Asn Pro Tyr Thr Asp Ala Ser
        335                 340                 345
Phe Ala Gln Ser Met Leu Lys Asn Ala Gln Ala Gln Ala Glu Met Phe
    350                 355                 360
Asn Leu Ser Glu Gln Val Lys Lys Asn Leu Glu Val Met Lys Asn Asn
365                 370                 375                 380
Asn Asn Val Asn Glu Lys Leu Ala Gly Phe Gly Lys Glu Glu Val Met
                385                 390                 395
Thr Asn Phe Val Ser Ala Phe Leu Ala Ser Cys Lys Asp Gly Gly Thr
            400                 405                 410
Leu Pro Asn Ala Gly Val Thr Ser Asn Thr Trp Gly Ala Gly Cys Ala
        415                 420                 425
Tyr Val Gly Glu Thr Ile Ser Ala Leu Thr Asn Ser Ile Ala His Phe
    430                 435                 440
Gly Thr Gln Glu Gln Gln Ile Gln Gln Ala Glu Asn Ile Ala Asp Thr
445                 450                 455                 460
Leu Val Asn Phe Lys Ser Arg Tyr Ser Glu Leu Gly Asn Thr Tyr Asn
                465                 470                 475
Ser Ile Thr Thr Ala Leu Ser Lys Val Pro Asn Ala Gln Ser Leu Gln
            480                 485                 490
Asn Val Val Ser Lys Lys Asn Asn Pro Tyr Ser Pro Gln Gly Ile Glu
        495                 500                 505
Thr Asn Tyr Tyr Leu Asn Gln Asn Ser Tyr Asn Gln Ile Gln Thr Ile
    510                 515                 520
Asn Gln Glu Leu Gly Arg Asn Pro Phe Arg Lys Val Gly Ile Val Asn
525                 530                 535                 540
Ser Gln Thr Asn Asn Gly Ala Met Asn Gly Ile Gly Ile Gln Val Gly
                545                 550                 555
Tyr Lys Gln Phe Phe Gly Gln Lys Arg Lys Trp Gly Ala Arg Tyr Tyr
            560                 565                 570
Gly Phe Phe Asp Tyr Asn His Ala Phe Ile Lys Ser Ser Phe Phe Asn
        575                 580                 585
Ser Ala Ser Asp Val Trp Thr Tyr Gly Phe Gly Ala Asp Ala Leu Tyr
    590                 595                 600
Asn Phe Ile Asn Asp Lys Ala Thr Asn Phe Leu Gly Lys Asn Asn Lys
605                 610                 615                 620
Leu Ser Leu Gly Leu Phe Gly Gly Ile Ala Leu Ala Gly Thr Ser Trp
                625                 630                 635
Leu Asn Ser Glu Tyr Val Asn Leu Ala Thr Val Asn Asn Val Tyr Asn
            640                 645                 650
Ala Lys Met Asn Val Ala Asn Phe Gln Phe Leu Phe Asn Met Gly Val
        655                 660                 665
Arg Met Asn Leu Ala Arg Ser Lys Lys Lys Gly Ser Asp His Ala Ala
    670                 675                 680
Gln His Gly Ile Glu Leu Gly Leu Lys Ile Pro Thr Ile Asn Thr Asn
685                 690                 695                 700
Tyr Tyr Ser Phe Met Gly Ala Glu Leu Lys Tyr Arg Arg Leu Tyr Ser
                705                 710                 715
Val Tyr Leu Asn Xaa Val Phe Ala Tyr
            720                 725
 
           
             7 
             2603 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (210)...(2342) 
             
             
               sig_peptide 
               (210)...(270) 
             
           
            7
atgaccttta ttggtttaat atttgtttag aaataacaca aaaacctttt tttttttttt     60
tgaaagggca aaaacgccta attaatatca aaatcccatg aatttatact atattaacga    120
aagcttgcgg tatggtttca cctaaagaca cacttccgca agatttacta acaatttcaa    180
tcttatttca agtaataaaa ggagaaaac atg aag aaa aaa ttt ctg tca tta      233
                                Met Lys Lys Lys Phe Leu Ser Leu
                                -20                 -15
acc tta ggt tcg ctt tta gtt tcc gct tta agc gct gaa gac aac ggc      281
Thr Leu Gly Ser Leu Leu Val Ser Ala Leu Ser Ala Glu Asp Asn Gly
        -10                  -5                 1
ttt ttt gtg agt gcg ggc tat caa atc ggt gaa tcc gct caa atg gtg      329
Phe Phe Val Ser Ala Gly Tyr Gln Ile Gly Glu Ser Ala Gln Met Val
 5                   10                  15                  20
aaa aac act aaa ggc att caa gat ctt tca gat agc tat gaa aga ctg      377
Lys Asn Thr Lys Gly Ile Gln Asp Leu Ser Asp Ser Tyr Glu Arg Leu
                 25                  30                  35
aac aat ctt tta acg agt tat agt gcc cta aac act ctt att agg cag      425
Asn Asn Leu Leu Thr Ser Tyr Ser Ala Leu Asn Thr Leu Ile Arg Gln
             40                  45                  50
tcc gcc gac ccc aac gct atc aat aac gca agg ggc aat ttg aac gct      473
Ser Ala Asp Pro Asn Ala Ile Asn Asn Ala Arg Gly Asn Leu Asn Ala
         55                  60                  65
agt gcg aag aat ttg atc aat gat aaa aag aat tcc ccg gcg tat caa      521
Ser Ala Lys Asn Leu Ile Asn Asp Lys Lys Asn Ser Pro Ala Tyr Gln
     70                  75                  80
gcg gtg ctt tta gcc ttg aat gcg gca gcg ggg ttg tgg caa gtc atg      569
Ala Val Leu Leu Ala Leu Asn Ala Ala Ala Gly Leu Trp Gln Val Met
 85                  90                  95                 100
agc tat tcg atc agc gtt tgt ggc cct ggc tct gac aaa aat aaa aat      617
Ser Tyr Ser Ile Ser Val Cys Gly Pro Gly Ser Asp Lys Asn Lys Asn
                105                 110                 115
ggg ggc gtc caa acc ttt gaa aat gtg ccg tca aat ggg ggg act acc      665
Gly Gly Val Gln Thr Phe Glu Asn Val Pro Ser Asn Gly Gly Thr Thr
            120                 125                 130
att gct tgc gat tca ttt tat gaa cca gga aag tgg agc ggt ata tcc      713
Ile Ala Cys Asp Ser Phe Tyr Glu Pro Gly Lys Trp Ser Gly Ile Ser
        135                 140                 145
act gaa aat tac gca aaa atc aat aaa gcc tat caa atc atc caa aag      761
Thr Glu Asn Tyr Ala Lys Ile Asn Lys Ala Tyr Gln Ile Ile Gln Lys
    150                 155                 160
gct ttt gga gca agc ggg caa gat att cct gcc tta agc gac acc aaa      809
Ala Phe Gly Ala Ser Gly Gln Asp Ile Pro Ala Leu Ser Asp Thr Lys
165                 170                 175                 180
gaa ctt aat ttt gaa att aaa ggg aaa aaa aat gat agc gtc cag cca      857
Glu Leu Asn Phe Glu Ile Lys Gly Lys Lys Asn Asp Ser Val Gln Pro
                185                 190                 195
gga gaa aga tgg aaa ttc cca tgg act aat gga aaa ttt gtt tca gtc      905
Gly Glu Arg Trp Lys Phe Pro Trp Thr Asn Gly Lys Phe Val Ser Val
            200                 205                 210
aag tgg gtg aat ggg aag tat gaa gaa att aaa gaa gac atc aaa gtg      953
Lys Trp Val Asn Gly Lys Tyr Glu Glu Ile Lys Glu Asp Ile Lys Val
        215                 220                 225
tca aat aac gct caa gag ctt tta aaa cag gct agc act att tta acc     1001
Ser Asn Asn Ala Gln Glu Leu Leu Lys Gln Ala Ser Thr Ile Leu Thr
    230                 235                 240
act ctt aat gaa gca tgc cca tgg ttg agt aat ggt ggt gca ggc aat     1049
Thr Leu Asn Glu Ala Cys Pro Trp Leu Ser Asn Gly Gly Ala Gly Asn
245                 250                 255                 260
gtg gcc ggt ggc aat agt tta tgg gcc gga ata gat aaa ggc gac ggg     1097
Val Ala Gly Gly Asn Ser Leu Trp Ala Gly Ile Asp Lys Gly Asp Gly
                265                 270                 275
agc gca tgc ggg att ttt aaa aat gaa atc agc gcg att caa gac atg     1145
Ser Ala Cys Gly Ile Phe Lys Asn Glu Ile Ser Ala Ile Gln Asp Met
            280                 285                 290
atc aaa aac gct gaa ata gcc gta gag caa tcc aaa atc gtt acc gcc     1193
Ile Lys Asn Ala Glu Ile Ala Val Glu Gln Ser Lys Ile Val Thr Ala
        295                 300                 305
aac gcg caa aac cag cac aac cta gac act ggg aaa gca ttc aac ccc     1241
Asn Ala Gln Asn Gln His Asn Leu Asp Thr Gly Lys Ala Phe Asn Pro
    310                 315                 320
tat aaa gac gcc aac ttc gcc caa agc atg ttc gct aac gct aga gcg     1289
Tyr Lys Asp Ala Asn Phe Ala Gln Ser Met Phe Ala Asn Ala Arg Ala
325                 330                 335                 340
caa gcg gag att tta aac cgc gct caa gca gtg gtg aag gac ttt gaa     1337
Gln Ala Glu Ile Leu Asn Arg Ala Gln Ala Val Val Lys Asp Phe Glu
                345                 350                 355
aga atc cct gca gcg ttc gtg aaa gac tct tta gga gta tgc cat gaa     1385
Arg Ile Pro Ala Ala Phe Val Lys Asp Ser Leu Gly Val Cys His Glu
            360                 365                 370
aag ggt agc gac ggc aat ctc cgt ggc acg cca tct ggc acg gtt act     1433
Lys Gly Ser Asp Gly Asn Leu Arg Gly Thr Pro Ser Gly Thr Val Thr
        375                 380                 385
tct aac act tgg gga gcc ggc tgc gcg tat gtg gga gaa acc gta acg     1481
Ser Asn Thr Trp Gly Ala Gly Cys Ala Tyr Val Gly Glu Thr Val Thr
    390                 395                 400
aat cta aaa aac agc atc gct cat ttt ggc gac caa gcg gag cga atc     1529
Asn Leu Lys Asn Ser Ile Ala His Phe Gly Asp Gln Ala Glu Arg Ile
405                 410                 415                 420
cat aat gcg cga aat ctc gcc tac act tta gcg aat ttc agc ggc cag     1577
His Asn Ala Arg Asn Leu Ala Tyr Thr Leu Ala Asn Phe Ser Gly Gln
                425                 430                 435
tac aaa aag cta ggc gaa cac tat gac agc atc aca gcg gcg ctc tct     1625
Tyr Lys Lys Leu Gly Glu His Tyr Asp Ser Ile Thr Ala Ala Leu Ser
            440                 445                 450
agc ttg cct gat gcg caa tct tta caa aat gtg gtg agc aaa aag act     1673
Ser Leu Pro Asp Ala Gln Ser Leu Gln Asn Val Val Ser Lys Lys Thr
        455                 460                 465
aac cct aac agc ccg caa ggc ata cag gat aat tac tac att gac tcc     1721
Asn Pro Asn Ser Pro Gln Gly Ile Gln Asp Asn Tyr Tyr Ile Asp Ser
    470                 475                 480
aac atc cat tct caa gtg caa tct agg agt caa gaa ctc ggc agt aac     1769
Asn Ile His Ser Gln Val Gln Ser Arg Ser Gln Glu Leu Gly Ser Asn
485                 490                 495                 500
cct ttc aga cgc gcc ggg cta atc gcc gct tct acc acc aat aac ggc     1817
Pro Phe Arg Arg Ala Gly Leu Ile Ala Ala Ser Thr Thr Asn Asn Gly
                505                 510                 515
gcg atg aat ggg att ggc ttt caa gtg ggc tat aag caa ttc ttt ggg     1865
Ala Met Asn Gly Ile Gly Phe Gln Val Gly Tyr Lys Gln Phe Phe Gly
            520                 525                 530
aaa aac aaa cga tgg ggc gcg aga tac tac ggc ttt gtg gat tac aac     1913
Lys Asn Lys Arg Trp Gly Ala Arg Tyr Tyr Gly Phe Val Asp Tyr Asn
        535                 540                 545
cac acc tat aac aag tcc caa ttt ttc aac tcc gat tct gat gtt tgg     1961
His Thr Tyr Asn Lys Ser Gln Phe Phe Asn Ser Asp Ser Asp Val Trp
    550                 555                 560
act tat ggc gtg ggg agc gat ttg tta gtg aat ttc atc aac gat aaa     2009
Thr Tyr Gly Val Gly Ser Asp Leu Leu Val Asn Phe Ile Asn Asp Lys
565                 570                 575                 580
gcc act aaa cac aat aaa att tct ttt ggc gcg ttt ggc ggt atc caa     2057
Ala Thr Lys His Asn Lys Ile Ser Phe Gly Ala Phe Gly Gly Ile Gln
                585                 590                 595
cta gcc ggg act tca tgg ctt aat tct cag tat gtg aat tta gcg aat     2105
Leu Ala Gly Thr Ser Trp Leu Asn Ser Gln Tyr Val Asn Leu Ala Asn
            600                 605                 610
gtg aac aat tat tat aaa gct aaa atc aac acc tct aac ttc caa ttc     2153
Val Asn Asn Tyr Tyr Lys Ala Lys Ile Asn Thr Ser Asn Phe Gln Phe
        615                 620                 625
tta ttc aat ctg ggc tta agg acc aat ctc gcc aga aat aaa aga ata     2201
Leu Phe Asn Leu Gly Leu Arg Thr Asn Leu Ala Arg Asn Lys Arg Ile
    630                 635                 640
ggc gct gat cat agc gcg caa cat ggc atg gaa tta ggc gtg aag atc     2249
Gly Ala Asp His Ser Ala Gln His Gly Met Glu Leu Gly Val Lys Ile
645                 650                 655                 660
ccc acg atc aac aca aat tac tat tct ttg cta ggc act acc ttg caa     2297
Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Leu Leu Gly Thr Thr Leu Gln
                665                 670                 675
tac aga agg ctt tat agc gtg tat ctc aac tat gtg ttt gct tac         2342
Tyr Arg Arg Leu Tyr Ser Val Tyr Leu Asn Tyr Val Phe Ala Tyr
            680                 685                 690
taaaagctta aactcctttt taaactccct ttttaggggg tttaatcttt ttaactgact   2402
tttcttttag ctttttttaa ttttttccac caaacaaagt tttttgactt caagcgttaa   2462
tcacaaaaaa tactcaaagg cgttttttgc aatctaaata aaaaattagc gttattcaag   2522
cgatcatttt aaaccaccca agcaagaaac cccaaacatc tttagcgttc gcgcgctcca   2582
ctaaccaaaa aacgccccaa a                                             2603
 
           
             8 
             711 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(20) 
             
           
            8
Met Lys Lys Lys Phe Leu Ser Leu Thr Leu Gly Ser Leu Leu Val Ser
-20                 -15                 -10                 -5
Ala Leu Ser Ala Glu Asp Asn Gly Phe Phe Val Ser Ala Gly Tyr Gln
                 1               5                  10
Ile Gly Glu Ser Ala Gln Met Val Lys Asn Thr Lys Gly Ile Gln Asp
        15                  20                  25
Leu Ser Asp Ser Tyr Glu Arg Leu Asn Asn Leu Leu Thr Ser Tyr Ser
    30                  35                  40
Ala Leu Asn Thr Leu Ile Arg Gln Ser Ala Asp Pro Asn Ala Ile Asn
45                  50                  55                  60
Asn Ala Arg Gly Asn Leu Asn Ala Ser Ala Lys Asn Leu Ile Asn Asp
                65                  70                  75
Lys Lys Asn Ser Pro Ala Tyr Gln Ala Val Leu Leu Ala Leu Asn Ala
            80                  85                  90
Ala Ala Gly Leu Trp Gln Val Met Ser Tyr Ser Ile Ser Val Cys Gly
        95                  100                 105
Pro Gly Ser Asp Lys Asn Lys Asn Gly Gly Val Gln Thr Phe Glu Asn
    110                 115                 120
Val Pro Ser Asn Gly Gly Thr Thr Ile Ala Cys Asp Ser Phe Tyr Glu
125                 130                 135                 140
Pro Gly Lys Trp Ser Gly Ile Ser Thr Glu Asn Tyr Ala Lys Ile Asn
                145                 150                 155
Lys Ala Tyr Gln Ile Ile Gln Lys Ala Phe Gly Ala Ser Gly Gln Asp
            160                 165                 170
Ile Pro Ala Leu Ser Asp Thr Lys Glu Leu Asn Phe Glu Ile Lys Gly
        175                 180                 185
Lys Lys Asn Asp Ser Val Gln Pro Gly Glu Arg Trp Lys Phe Pro Trp
    190                 195                 200
Thr Asn Gly Lys Phe Val Ser Val Lys Trp Val Asn Gly Lys Tyr Glu
205                 210                 215                 220
Glu Ile Lys Glu Asp Ile Lys Val Ser Asn Asn Ala Gln Glu Leu Leu
                225                 230                 235
Lys Gln Ala Ser Thr Ile Leu Thr Thr Leu Asn Glu Ala Cys Pro Trp
            240                 245                 250
Leu Ser Asn Gly Gly Ala Gly Asn Val Ala Gly Gly Asn Ser Leu Trp
        255                 260                 265
Ala Gly Ile Asp Lys Gly Asp Gly Ser Ala Cys Gly Ile Phe Lys Asn
    270                 275                 280
Glu Ile Ser Ala Ile Gln Asp Met Ile Lys Asn Ala Glu Ile Ala Val
285                 290                 295                 300
Glu Gln Ser Lys Ile Val Thr Ala Asn Ala Gln Asn Gln His Asn Leu
                305                 310                 315
Asp Thr Gly Lys Ala Phe Asn Pro Tyr Lys Asp Ala Asn Phe Ala Gln
            320                 325                 330
Ser Met Phe Ala Asn Ala Arg Ala Gln Ala Glu Ile Leu Asn Arg Ala
        335                 340                 345
Gln Ala Val Val Lys Asp Phe Glu Arg Ile Pro Ala Ala Phe Val Lys
    350                 355                 360
Asp Ser Leu Gly Val Cys His Glu Lys Gly Ser Asp Gly Asn Leu Arg
365                 370                 375                 380
Gly Thr Pro Ser Gly Thr Val Thr Ser Asn Thr Trp Gly Ala Gly Cys
                385                 390                 395
Ala Tyr Val Gly Glu Thr Val Thr Asn Leu Lys Asn Ser Ile Ala His
            400                 405                 410
Phe Gly Asp Gln Ala Glu Arg Ile His Asn Ala Arg Asn Leu Ala Tyr
        415                 420                 425
Thr Leu Ala Asn Phe Ser Gly Gln Tyr Lys Lys Leu Gly Glu His Tyr
    430                 435                 440
Asp Ser Ile Thr Ala Ala Leu Ser Ser Leu Pro Asp Ala Gln Ser Leu
445                 450                 455                 460
Gln Asn Val Val Ser Lys Lys Thr Asn Pro Asn Ser Pro Gln Gly Ile
                465                 470                 475
Gln Asp Asn Tyr Tyr Ile Asp Ser Asn Ile His Ser Gln Val Gln Ser
            480                 485                 490
Arg Ser Gln Glu Leu Gly Ser Asn Pro Phe Arg Arg Ala Gly Leu Ile
        495                 500                 505
Ala Ala Ser Thr Thr Asn Asn Gly Ala Met Asn Gly Ile Gly Phe Gln
    510                 515                 520
Val Gly Tyr Lys Gln Phe Phe Gly Lys Asn Lys Arg Trp Gly Ala Arg
525                 530                 535                 540
Tyr Tyr Gly Phe Val Asp Tyr Asn His Thr Tyr Asn Lys Ser Gln Phe
                545                 550                 555
Phe Asn Ser Asp Ser Asp Val Trp Thr Tyr Gly Val Gly Ser Asp Leu
            560                 565                 570
Leu Val Asn Phe Ile Asn Asp Lys Ala Thr Lys His Asn Lys Ile Ser
        575                 580                 585
Phe Gly Ala Phe Gly Gly Ile Gln Leu Ala Gly Thr Ser Trp Leu Asn
    590                 595                 600
Ser Gln Tyr Val Asn Leu Ala Asn Val Asn Asn Tyr Tyr Lys Ala Lys
605                 610                 615                 620
Ile Asn Thr Ser Asn Phe Gln Phe Leu Phe Asn Leu Gly Leu Arg Thr
                625                 630                 635
Asn Leu Ala Arg Asn Lys Arg Ile Gly Ala Asp His Ser Ala Gln His
            640                 645                 650
Gly Met Glu Leu Gly Val Lys Ile Pro Thr Ile Asn Thr Asn Tyr Tyr
        655                 660                 665
Ser Leu Leu Gly Thr Thr Leu Gln Tyr Arg Arg Leu Tyr Ser Val Tyr
    670                 675                 680
Leu Asn Tyr Val Phe Ala Tyr
685                 690
 
           
             9 
             2427 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (232)...(2247) 
             
             
               sig_peptide 
               (232)...(292) 
             
           
            9
aaaacgcgca gcaaaaaatc tctgttaagc ttttatcatt agcgttccat tgaaacaaaa     60
tctaaaaacc ctttccaata ccacccaaac aaacgcgcaa aaaatgcaaa aattctaaat    120
tttctccaaa tgacaaaaaa aaaaaaaacg attttatgct acaatgcttt taatacattc    180
ttacttaatg tataaaatct caatcactca atttaatttc aaaggatatt t atg aaa     237
                                                         Met Lys
                                                         -20
aaa acc ctt tta ctc tct ctc tct ctc tct ctc tcg tca tcg ctt tta      285
Lys Thr Leu Leu Leu Ser Leu Ser Leu Ser Leu Ser Ser Ser Leu Leu
            -15                 -10                  -5
aac gct gaa gac aac ggc ttt ttt atc agc gcg ggc tat caa atc ggt      333
Asn Ala Glu Asp Asn Gly Phe Phe Ile Ser Ala Gly Tyr Gln Ile Gly
        1               5                   10
gaa gcc gct caa atg gtg aaa aac acc ggc gaa ttg aaa aaa ctt tca      381
Glu Ala Ala Gln Met Val Lys Asn Thr Gly Glu Leu Lys Lys Leu Ser
 15                  20                  25                  30
gac act tat gag aat ttg agc aac ctt tta acc aat ttt aac aac ctc      429
Asp Thr Tyr Glu Asn Leu Ser Asn Leu Leu Thr Asn Phe Asn Asn Leu
                 35                  40                  45
aat caa gcg gta acg aac gcg agc agc cct tca gaa atc aat gcc acg      477
Asn Gln Ala Val Thr Asn Ala Ser Ser Pro Ser Glu Ile Asn Ala Thr
             50                  55                  60
atc gat aat tta aaa gca aac acg caa ggg ctg att ggc gaa aaa acc      525
Ile Asp Asn Leu Lys Ala Asn Thr Gln Gly Leu Ile Gly Glu Lys Thr
         65                  70                  75
aat tcc ccg gcg tat caa gcg gtg tat ttg gcg ctc aat gcg gcg gtg      573
Asn Ser Pro Ala Tyr Gln Ala Val Tyr Leu Ala Leu Asn Ala Ala Val
     80                  85                  90
ggg ctg tgg aat gtg ata gcc tat aat gtc caa tgc ggt cct ggt aag      621
Gly Leu Trp Asn Val Ile Ala Tyr Asn Val Gln Cys Gly Pro Gly Lys
 95                 100                 105                 110
agt ggg gat caa agc gta att ttt gat ggc caa cca gga cat gat tca      669
Ser Gly Asp Gln Ser Val Ile Phe Asp Gly Gln Pro Gly His Asp Ser
                115                 120                 125
aga tcc att aat tgc aat tta acc ggt tat aac aac ggg gtt agc ggc      717
Arg Ser Ile Asn Cys Asn Leu Thr Gly Tyr Asn Asn Gly Val Ser Gly
            130                 135                 140
cct tta tcc att gac aat ttt aaa acg ctt aat caa gct tat caa act      765
Pro Leu Ser Ile Asp Asn Phe Lys Thr Leu Asn Gln Ala Tyr Gln Thr
        145                 150                 155
atc caa caa gct tta aaa caa gat agc gga ttt cct gtt ttg gat agt      813
Ile Gln Gln Ala Leu Lys Gln Asp Ser Gly Phe Pro Val Leu Asp Ser
    160                 165                 170
aaa gga aaa caa gta act ata aaa ata aca aca caa act aat gga gct      861
Lys Gly Lys Gln Val Thr Ile Lys Ile Thr Thr Gln Thr Asn Gly Ala
175                 180                 185                 190
aat aaa agt gaa act act act act act act act act aat gac gct caa      909
Asn Lys Ser Glu Thr Thr Thr Thr Thr Thr Thr Thr Asn Asp Ala Gln
                195                 200                 205
acc ctt ttg caa gaa gcc agt aaa atg ata agc gtc ctc act aca aac      957
Thr Leu Leu Gln Glu Ala Ser Lys Met Ile Ser Val Leu Thr Thr Asn
            210                 215                 220
tgc cca tgg gta aat acc gct cat aac tca aac ggg ggt gca ccg tgg     1005
Cys Pro Trp Val Asn Thr Ala His Asn Ser Asn Gly Gly Ala Pro Trp
        225                 230                 235
aat tta aat acg aca ggg aat gtg tgt cag gtt ttt gcc acg gag ttt     1053
Asn Leu Asn Thr Thr Gly Asn Val Cys Gln Val Phe Ala Thr Glu Phe
    240                 245                 250
agc gcc gtt act agc atg atc aaa aac gcg caa gaa atc gta acg caa     1101
Ser Ala Val Thr Ser Met Ile Lys Asn Ala Gln Glu Ile Val Thr Gln
255                 260                 265                 270
gct caa agc ctt aac aac ccg caa agc aat caa aac gcg ccg aaa gat     1149
Ala Gln Ser Leu Asn Asn Pro Gln Ser Asn Gln Asn Ala Pro Lys Asp
                275                 280                 285
ttc aat cct tac acc tct gct gat agg gct ttc gct caa aac atg ctc     1197
Phe Asn Pro Tyr Thr Ser Ala Asp Arg Ala Phe Ala Gln Asn Met Leu
            290                 295                 300
aat cac gcg caa gcg caa gcc aag atg ctt gaa cta gcc gat caa atg     1245
Asn His Ala Gln Ala Gln Ala Lys Met Leu Glu Leu Ala Asp Gln Met
        305                 310                 315
aaa aaa gac ctt aac act atc cca aaa caa ttt atc aca aac tac ttg     1293
Lys Lys Asp Leu Asn Thr Ile Pro Lys Gln Phe Ile Thr Asn Tyr Leu
    320                 325                 330
gca gct tgc cgc aat ggg ggt ggg aca tta cct gat gca ggg gtt act     1341
Ala Ala Cys Arg Asn Gly Gly Gly Thr Leu Pro Asp Ala Gly Val Thr
335                 340                 345                 350
tct aac act tgg ggg gcc ggt tgc gcc tat gtg gaa gag acg ata acc     1389
Ser Asn Thr Trp Gly Ala Gly Cys Ala Tyr Val Glu Glu Thr Ile Thr
                355                 360                 365
gcc cta aat aac agc ctt gcg cat ttt ggc act caa gcc gat caa atc     1437
Ala Leu Asn Asn Ser Leu Ala His Phe Gly Thr Gln Ala Asp Gln Ile
            370                 375                 380
aag caa tct gag ttg ttg gcg cgc acg ata ctt gat ttt aga ggc agc     1485
Lys Gln Ser Glu Leu Leu Ala Arg Thr Ile Leu Asp Phe Arg Gly Ser
        385                 390                 395
ctt aag gat tta aac aac act tat aac agc atc acc acg acc gct tca     1533
Leu Lys Asp Leu Asn Asn Thr Tyr Asn Ser Ile Thr Thr Thr Ala Ser
    400                 405                 410
aac acg ccc aat tcc cca ttc ctt aaa aat ttg ata agc caa tcc act     1581
Asn Thr Pro Asn Ser Pro Phe Leu Lys Asn Leu Ile Ser Gln Ser Thr
415                 420                 425                 430
aac cct aat aac ccc ggg ggc tta cag gcc gtt tat caa gtc aac caa     1629
Asn Pro Asn Asn Pro Gly Gly Leu Gln Ala Val Tyr Gln Val Asn Gln
                435                 440                 445
agc gct tat tcg caa tta tta agc gcc acg caa gaa tta ggg cat aac     1677
Ser Ala Tyr Ser Gln Leu Leu Ser Ala Thr Gln Glu Leu Gly His Asn
            450                 455                 460
cct ttc aga cgc gtt ggc tta atc agc tct caa acc aac aac ggt gcg     1725
Pro Phe Arg Arg Val Gly Leu Ile Ser Ser Gln Thr Asn Asn Gly Ala
        465                 470                 475
atg aat ggg atc ggc gtg caa ata ggg tat aaa caa ttt ttt ggt gaa     1773
Met Asn Gly Ile Gly Val Gln Ile Gly Tyr Lys Gln Phe Phe Gly Glu
    480                 485                 490
aaa aga aga tgg ggg tta agg tat tat ggt ttt ttt gat tac aac cat     1821
Lys Arg Arg Trp Gly Leu Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn His
495                 500                 505                 510
gct tat atc aaa tcc agc ttt ttc aac tcc gcc tct gat gtg ttc act     1869
Ala Tyr Ile Lys Ser Ser Phe Phe Asn Ser Ala Ser Asp Val Phe Thr
                515                 520                 525
tat ggg gta gga aca gat gtc ctc tat aac ttt atc aac gat aaa gcc     1917
Tyr Gly Val Gly Thr Asp Val Leu Tyr Asn Phe Ile Asn Asp Lys Ala
            530                 535                 540
acc aaa aac aat aag att tct ttt ggg gtg ttt ggg ggg att gcg tta     1965
Thr Lys Asn Asn Lys Ile Ser Phe Gly Val Phe Gly Gly Ile Ala Leu
        545                 550                 555
gct ggc act tcg tgg ctt aat tct caa tac gtg aat tta gcg aca ttc     2013
Ala Gly Thr Ser Trp Leu Asn Ser Gln Tyr Val Asn Leu Ala Thr Phe
    560                 565                 570
aat aat ttt tac agc gct aaa atg aat gtg gcg aat ttc caa ttc tta     2061
Asn Asn Phe Tyr Ser Ala Lys Met Asn Val Ala Asn Phe Gln Phe Leu
575                 580                 585                 590
ttc aac ttg ggc ttg aga atg aat ctc gct aaa aac aaa aag aaa gcg     2109
Phe Asn Leu Gly Leu Arg Met Asn Leu Ala Lys Asn Lys Lys Lys Ala
                595                 600                 605
agc gat cat gta gct cag cat ggc gtg gaa cta ggc gtg aag atc cct     2157
Ser Asp His Val Ala Gln His Gly Val Glu Leu Gly Val Lys Ile Pro
            610                 615                 620
acg atc aac acg aat tac tat tct ttg cta ggc act caa ctc caa tac     2205
Thr Ile Asn Thr Asn Tyr Tyr Ser Leu Leu Gly Thr Gln Leu Gln Tyr
        625                 630                 635
cgc agg ctt tat agc gtg tat ttg aat tat gtg ttt gct tac             2247
Arg Arg Leu Tyr Ser Val Tyr Leu Asn Tyr Val Phe Ala Tyr
    640                 645                 650
taatatctgt ctttttgtga aactcccttt ttaagggatt ttttttgaag cctttctttt   2307
tttaaaccct cttttttggg ggtcaagcgt aaaattcacc cctatccctt taagaaaata   2367
aaataaaaga aaatgcgttt tataacaaaa taagatctaa aacaataaaa caaaaaccca   2427
 
           
             10 
             672 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(20) 
             
           
            10
Met Lys Lys Thr Leu Leu Leu Ser Leu Ser Leu Ser Leu Ser Ser Ser
-20                 -15                 -10                 -5
Leu Leu Asn Ala Glu Asp Asn Gly Phe Phe Ile Ser Ala Gly Tyr Gln
                 1               5                  10
Ile Gly Glu Ala Ala Gln Met Val Lys Asn Thr Gly Glu Leu Lys Lys
        15                  20                  25
Leu Ser Asp Thr Tyr Glu Asn Leu Ser Asn Leu Leu Thr Asn Phe Asn
    30                  35                  40
Asn Leu Asn Gln Ala Val Thr Asn Ala Ser Ser Pro Ser Glu Ile Asn
45                  50                  55                  60
Ala Thr Ile Asp Asn Leu Lys Ala Asn Thr Gln Gly Leu Ile Gly Glu
                65                  70                  75
Lys Thr Asn Ser Pro Ala Tyr Gln Ala Val Tyr Leu Ala Leu Asn Ala
            80                  85                  90
Ala Val Gly Leu Trp Asn Val Ile Ala Tyr Asn Val Gln Cys Gly Pro
        95                  100                 105
Gly Lys Ser Gly Asp Gln Ser Val Ile Phe Asp Gly Gln Pro Gly His
    110                 115                 120
Asp Ser Arg Ser Ile Asn Cys Asn Leu Thr Gly Tyr Asn Asn Gly Val
125                 130                 135                 140
Ser Gly Pro Leu Ser Ile Asp Asn Phe Lys Thr Leu Asn Gln Ala Tyr
                145                 150                 155
Gln Thr Ile Gln Gln Ala Leu Lys Gln Asp Ser Gly Phe Pro Val Leu
            160                 165                 170
Asp Ser Lys Gly Lys Gln Val Thr Ile Lys Ile Thr Thr Gln Thr Asn
        175                 180                 185
Gly Ala Asn Lys Ser Glu Thr Thr Thr Thr Thr Thr Thr Thr Asn Asp
    190                 195                 200
Ala Gln Thr Leu Leu Gln Glu Ala Ser Lys Met Ile Ser Val Leu Thr
205                 210                 215                 220
Thr Asn Cys Pro Trp Val Asn Thr Ala His Asn Ser Asn Gly Gly Ala
                225                 230                 235
Pro Trp Asn Leu Asn Thr Thr Gly Asn Val Cys Gln Val Phe Ala Thr
            240                 245                 250
Glu Phe Ser Ala Val Thr Ser Met Ile Lys Asn Ala Gln Glu Ile Val
        255                 260                 265
Thr Gln Ala Gln Ser Leu Asn Asn Pro Gln Ser Asn Gln Asn Ala Pro
    270                 275                 280
Lys Asp Phe Asn Pro Tyr Thr Ser Ala Asp Arg Ala Phe Ala Gln Asn
285                 290                 295                 300
Met Leu Asn His Ala Gln Ala Gln Ala Lys Met Leu Glu Leu Ala Asp
                305                 310                 315
Gln Met Lys Lys Asp Leu Asn Thr Ile Pro Lys Gln Phe Ile Thr Asn
            320                 325                 330
Tyr Leu Ala Ala Cys Arg Asn Gly Gly Gly Thr Leu Pro Asp Ala Gly
        335                 340                 345
Val Thr Ser Asn Thr Trp Gly Ala Gly Cys Ala Tyr Val Glu Glu Thr
    350                 355                 360
Ile Thr Ala Leu Asn Asn Ser Leu Ala His Phe Gly Thr Gln Ala Asp
365                 370                 375                 380
Gln Ile Lys Gln Ser Glu Leu Leu Ala Arg Thr Ile Leu Asp Phe Arg
                385                 390                 395
Gly Ser Leu Lys Asp Leu Asn Asn Thr Tyr Asn Ser Ile Thr Thr Thr
            400                 405                 410
Ala Ser Asn Thr Pro Asn Ser Pro Phe Leu Lys Asn Leu Ile Ser Gln
        415                 420                 425
Ser Thr Asn Pro Asn Asn Pro Gly Gly Leu Gln Ala Val Tyr Gln Val
    430                 435                 440
Asn Gln Ser Ala Tyr Ser Gln Leu Leu Ser Ala Thr Gln Glu Leu Gly
445                 450                 455                 460
His Asn Pro Phe Arg Arg Val Gly Leu Ile Ser Ser Gln Thr Asn Asn
                465                 470                 475
Gly Ala Met Asn Gly Ile Gly Val Gln Ile Gly Tyr Lys Gln Phe Phe
            480                 485                 490
Gly Glu Lys Arg Arg Trp Gly Leu Arg Tyr Tyr Gly Phe Phe Asp Tyr
        495                 500                 505
Asn His Ala Tyr Ile Lys Ser Ser Phe Phe Asn Ser Ala Ser Asp Val
    510                 515                 520
Phe Thr Tyr Gly Val Gly Thr Asp Val Leu Tyr Asn Phe Ile Asn Asp
525                 530                 535                 540
Lys Ala Thr Lys Asn Asn Lys Ile Ser Phe Gly Val Phe Gly Gly Ile
                545                 550                 555
Ala Leu Ala Gly Thr Ser Trp Leu Asn Ser Gln Tyr Val Asn Leu Ala
            560                 565                 570
Thr Phe Asn Asn Phe Tyr Ser Ala Lys Met Asn Val Ala Asn Phe Gln
        575                 580                 585
Phe Leu Phe Asn Leu Gly Leu Arg Met Asn Leu Ala Lys Asn Lys Lys
    590                 595                 600
Lys Ala Ser Asp His Val Ala Gln His Gly Val Glu Leu Gly Val Lys
605                 610                 615                 620
Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Leu Leu Gly Thr Gln Leu
                625                 630                 635
Gln Tyr Arg Arg Leu Tyr Ser Val Tyr Leu Asn Tyr Val Phe Ala Tyr
            640                 645                 650
 
           
             11 
             2429 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (205)...(2277) 
             
             
               sig_peptide 
               (205)...(259) 
             
           
            11
tgaaagaaga ctgattagtc tttcttttag gggcgattca agccttaaaa gccgggtcaa     60
aatccccatt tttcccaatt tttacaaaaa aaaaaaaaac aaaatctcta aaatttagag    120
ctaaaattag ccataaaatt ccatttattg cttataatat gaagtttctt tgtatcaaag    180
aaaaatctat taaaaggaga aaac atg aaa aaa tcc ctc tta ctc tct ctt       231
                           Met Lys Lys Ser Leu Leu Leu Ser Leu
                                       -15                 -10
tct ctc atc gct tcc tta tca aga gct gaa gat gac gga ttt tat acg      279
Ser Leu Ile Ala Ser Leu Ser Arg Ala Glu Asp Asp Gly Phe Tyr Thr
                 -5                 1               5
agt gtg ggc tat cag atc ggt gaa gcg gtc caa caa gtg aaa aac aca      327
Ser Val Gly Tyr Gln Ile Gly Glu Ala Val Gln Gln Val Lys Asn Thr
         10                  15                  20
gga gca ttg caa aat ctt gca gac aga tac gat aac tta aac aac ctt      375
Gly Ala Leu Gln Asn Leu Ala Asp Arg Tyr Asp Asn Leu Asn Asn Leu
     25                  30                  35
tta aac caa tac aat tat tta aat tcc tta gtc aat tta gcc agc acg      423
Leu Asn Gln Tyr Asn Tyr Leu Asn Ser Leu Val Asn Leu Ala Ser Thr
 40                  45                  50                  55
ccg agc gcg atc acc ggt gcg att gat aat tta agc tca agc gcg att      471
Pro Ser Ala Ile Thr Gly Ala Ile Asp Asn Leu Ser Ser Ser Ala Ile
                 60                  65                  70
aac ctc act agc gcc acc acc act tcc ccc gcc tat caa gct gtg gct      519
Asn Leu Thr Ser Ala Thr Thr Thr Ser Pro Ala Tyr Gln Ala Val Ala
             75                  80                  85
tta gcg ctc aat gcc gct gtg ggc atg tgg caa gtc ata gcc ctt ttt      567
Leu Ala Leu Asn Ala Ala Val Gly Met Trp Gln Val Ile Ala Leu Phe
         90                  95                 100
att ggc tgt ggc cct ggc cct acc aat aat caa agc tat caa tcg ttt      615
Ile Gly Cys Gly Pro Gly Pro Thr Asn Asn Gln Ser Tyr Gln Ser Phe
    105                 110                 115
ggt aac aca cca gcc ctt aat ggg acc acc acc act tgc aat caa gca      663
Gly Asn Thr Pro Ala Leu Asn Gly Thr Thr Thr Thr Cys Asn Gln Ala
120                 125                 130                 135
tat ggg aca ggc cct aat ggc atc cta tct att gat gaa tac caa aaa      711
Tyr Gly Thr Gly Pro Asn Gly Ile Leu Ser Ile Asp Glu Tyr Gln Lys
                140                 145                 150
ctc aac caa gct tat cag atc atc caa acc gct tta aac caa aat caa      759
Leu Asn Gln Ala Tyr Gln Ile Ile Gln Thr Ala Leu Asn Gln Asn Gln
            155                 160                 165
ggg ggt ggg atg cct gcc ttg aat gac acc acc aaa aca ggg gta gtc      807
Gly Gly Gly Met Pro Ala Leu Asn Asp Thr Thr Lys Thr Gly Val Val
        170                 175                 180
aac ata caa caa acc aat tat agg acc acc aca caa aac aat atc ata      855
Asn Ile Gln Gln Thr Asn Tyr Arg Thr Thr Thr Gln Asn Asn Ile Ile
    185                 190                 195
gag cat tat tat aca gag aat ggg aaa gag atc cca gtc tct tat tca      903
Glu His Tyr Tyr Thr Glu Asn Gly Lys Glu Ile Pro Val Ser Tyr Ser
200                 205                 210                 215
ggc gga tca tca ttc tcg cct aca ata caa ttg aca tac cat aat aac      951
Gly Gly Ser Ser Phe Ser Pro Thr Ile Gln Leu Thr Tyr His Asn Asn
                220                 225                 230
gct gaa aac ctt ttg caa caa gcc gcc act atc atg caa gtc ctt att      999
Ala Glu Asn Leu Leu Gln Gln Ala Ala Thr Ile Met Gln Val Leu Ile
            235                 240                 245
act caa aag ccg cat gtg caa acg agc aat ggc ggt aaa gcg tgg ggg     1047
Thr Gln Lys Pro His Val Gln Thr Ser Asn Gly Gly Lys Ala Trp Gly
        250                 255                 260
ttg agt tct acg cct ggg aat gtg atg gat att ttt ggt cct tct ttt     1095
Leu Ser Ser Thr Pro Gly Asn Val Met Asp Ile Phe Gly Pro Ser Phe
    265                 270                 275
aac gct att aat gag atg att aaa aac gct caa aca gcc cta gca aaa     1143
Asn Ala Ile Asn Glu Met Ile Lys Asn Ala Gln Thr Ala Leu Ala Lys
280                 285                 290                 295
acc caa cag ctt aac gct aat gaa aac gcc caa atc acg caa ccc aac     1191
Thr Gln Gln Leu Asn Ala Asn Glu Asn Ala Gln Ile Thr Gln Pro Asn
                300                 305                 310
aat ttc aac ccc tac acc tct aaa gac aaa ggg ttc gct caa gaa atg     1239
Asn Phe Asn Pro Tyr Thr Ser Lys Asp Lys Gly Phe Ala Gln Glu Met
            315                 320                 325
ctc aat aga gct gaa gct caa gca gag att tta aat tta gct aag caa     1287
Leu Asn Arg Ala Glu Ala Gln Ala Glu Ile Leu Asn Leu Ala Lys Gln
        330                 335                 340
gta gcg aac aat ttc cac agc att caa ggg cct att caa ggg gat tta     1335
Val Ala Asn Asn Phe His Ser Ile Gln Gly Pro Ile Gln Gly Asp Leu
    345                 350                 355
gaa gaa tgt aaa gca gga tcg gct ggc gtg atc act aat aac act tgg     1383
Glu Glu Cys Lys Ala Gly Ser Ala Gly Val Ile Thr Asn Asn Thr Trp
360                 365                 370                 375
ggt tca ggt tgc gcg ttt gtg aaa gaa act tta aac tct tta gag caa     1431
Gly Ser Gly Cys Ala Phe Val Lys Glu Thr Leu Asn Ser Leu Glu Gln
                380                 385                 390
cac acc gct tat tac ggc aac cag gtc aat cag gat agg gct ttg gct     1479
His Thr Ala Tyr Tyr Gly Asn Gln Val Asn Gln Asp Arg Ala Leu Ala
            395                 400                 405
caa acc att ttg aat ttt aaa gaa gcc ctt aac acc ctg aat aaa gac     1527
Gln Thr Ile Leu Asn Phe Lys Glu Ala Leu Asn Thr Leu Asn Lys Asp
        410                 415                 420
tca aaa gcg atc aat agc ggt atc tcc aac ttg cct aac gct aaa tct     1575
Ser Lys Ala Ile Asn Ser Gly Ile Ser Asn Leu Pro Asn Ala Lys Ser
    425                 430                 435
ctt caa aac atg acg cat gcc act caa aac cct aat tcc cca gaa ggt     1623
Leu Gln Asn Met Thr His Ala Thr Gln Asn Pro Asn Ser Pro Glu Gly
440                 445                 450                 455
ctg ctc act tat tct ttg gat tca agc aaa tac aac cag ctc caa acc     1671
Leu Leu Thr Tyr Ser Leu Asp Ser Ser Lys Tyr Asn Gln Leu Gln Thr
                460                 465                 470
atc gcg caa gaa ttg ggc aaa aac cct ttc agg cgc ttt ggc gtg att     1719
Ile Ala Gln Glu Leu Gly Lys Asn Pro Phe Arg Arg Phe Gly Val Ile
            475                 480                 485
gac ttt caa aac aac aac ggc gca atg aac ggg atc ggc gtg caa gtg     1767
Asp Phe Gln Asn Asn Asn Gly Ala Met Asn Gly Ile Gly Val Gln Val
        490                 495                 500
ggt tat aaa caa ttc ttt ggt aaa aaa agg aat tgg ggg tta agg tat     1815
Gly Tyr Lys Gln Phe Phe Gly Lys Lys Arg Asn Trp Gly Leu Arg Tyr
    505                 510                 515
tat ggt ttc ttt gat tat aac cat gct tat atc aaa tct aat ttt ttc     1863
Tyr Gly Phe Phe Asp Tyr Asn His Ala Tyr Ile Lys Ser Asn Phe Phe
520                 525                 530                 535
aac tcc gct tct gat gtg tgg act tat ggg gtg ggt atg gac gct ctc     1911
Asn Ser Ala Ser Asp Val Trp Thr Tyr Gly Val Gly Met Asp Ala Leu
                540                 545                 550
tat aac ttc atc aac gat aaa aac acc aac ttt tta ggc aag aac aac     1959
Tyr Asn Phe Ile Asn Asp Lys Asn Thr Asn Phe Leu Gly Lys Asn Asn
            555                 560                 565
aag ctt tca gta ggg ctt ttt gga ggc ttt gcg tta gcc ggg act tcg     2007
Lys Leu Ser Val Gly Leu Phe Gly Gly Phe Ala Leu Ala Gly Thr Ser
        570                 575                 580
tgg ctt aat tcc caa caa gtg aat ttg acc atg atg aat ggc att tat     2055
Trp Leu Asn Ser Gln Gln Val Asn Leu Thr Met Met Asn Gly Ile Tyr
    585                 590                 595
aac gct aat gtc agc act tct aac ttc caa ttt ttg ttt gat tta ggc     2103
Asn Ala Asn Val Ser Thr Ser Asn Phe Gln Phe Leu Phe Asp Leu Gly
600                 605                 610                 615
ttg aga atg aac ctc gct agg cct aag aaa aaa gac agc gat cat gcc     2151
Leu Arg Met Asn Leu Ala Arg Pro Lys Lys Lys Asp Ser Asp His Ala
                620                 625                 630
gct cag cat ggc att gaa cta ggt ttt aag atc ccc acg atc aac acc     2199
Ala Gln His Gly Ile Glu Leu Gly Phe Lys Ile Pro Thr Ile Asn Thr
            635                 640                 645
aac tat tat tct ttc atg ggc gct aaa cta gaa tac aga agg atg tat     2247
Asn Tyr Tyr Ser Phe Met Gly Ala Lys Leu Glu Tyr Arg Arg Met Tyr
        650                 655                 660
agc ctt ttt ctc aat tat gtg ttt gct tac taaaaattct ttttgaaccc       2297
Ser Leu Phe Leu Asn Tyr Val Phe Ala Tyr
    665                 670
ctcttttttt gggggagtgt tgcaaaaatg cccccctatt tgcttgtgag ttttggttaa   2357
aattttagtt acccacgctt aaaaagcgcc aagcctttta cacacaactc ctttaatttt   2417
gtttttaaga aa                                                       2429
 
           
             12 
             691 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(18) 
             
           
            12
Met Lys Lys Ser Leu Leu Leu Ser Leu Ser Leu Ile Ala Ser Leu Ser
            -15                 -10                 -5
Arg Ala Glu Asp Asp Gly Phe Tyr Thr Ser Val Gly Tyr Gln Ile Gly
         1               5                  10
Glu Ala Val Gln Gln Val Lys Asn Thr Gly Ala Leu Gln Asn Leu Ala
15                  20                  25                  30
Asp Arg Tyr Asp Asn Leu Asn Asn Leu Leu Asn Gln Tyr Asn Tyr Leu
                35                  40                  45
Asn Ser Leu Val Asn Leu Ala Ser Thr Pro Ser Ala Ile Thr Gly Ala
            50                  55                  60
Ile Asp Asn Leu Ser Ser Ser Ala Ile Asn Leu Thr Ser Ala Thr Thr
        65                  70                  75
Thr Ser Pro Ala Tyr Gln Ala Val Ala Leu Ala Leu Asn Ala Ala Val
    80                  85                  90
Gly Met Trp Gln Val Ile Ala Leu Phe Ile Gly Cys Gly Pro Gly Pro
95                  100                 105                 110
Thr Asn Asn Gln Ser Tyr Gln Ser Phe Gly Asn Thr Pro Ala Leu Asn
                115                 120                 125
Gly Thr Thr Thr Thr Cys Asn Gln Ala Tyr Gly Thr Gly Pro Asn Gly
            130                 135                 140
Ile Leu Ser Ile Asp Glu Tyr Gln Lys Leu Asn Gln Ala Tyr Gln Ile
        145                 150                 155
Ile Gln Thr Ala Leu Asn Gln Asn Gln Gly Gly Gly Met Pro Ala Leu
    160                 165                 170
Asn Asp Thr Thr Lys Thr Gly Val Val Asn Ile Gln Gln Thr Asn Tyr
175                 180                 185                 190
Arg Thr Thr Thr Gln Asn Asn Ile Ile Glu His Tyr Tyr Thr Glu Asn
                195                 200                 205
Gly Lys Glu Ile Pro Val Ser Tyr Ser Gly Gly Ser Ser Phe Ser Pro
            210                 215                 220
Thr Ile Gln Leu Thr Tyr His Asn Asn Ala Glu Asn Leu Leu Gln Gln
        225                 230                 235
Ala Ala Thr Ile Met Gln Val Leu Ile Thr Gln Lys Pro His Val Gln
    240                 245                 250
Thr Ser Asn Gly Gly Lys Ala Trp Gly Leu Ser Ser Thr Pro Gly Asn
255                 260                 265                 270
Val Met Asp Ile Phe Gly Pro Ser Phe Asn Ala Ile Asn Glu Met Ile
                275                 280                 285
Lys Asn Ala Gln Thr Ala Leu Ala Lys Thr Gln Gln Leu Asn Ala Asn
            290                 295                 300
Glu Asn Ala Gln Ile Thr Gln Pro Asn Asn Phe Asn Pro Tyr Thr Ser
        305                 310                 315
Lys Asp Lys Gly Phe Ala Gln Glu Met Leu Asn Arg Ala Glu Ala Gln
    320                 325                 330
Ala Glu Ile Leu Asn Leu Ala Lys Gln Val Ala Asn Asn Phe His Ser
335                 340                 345                 350
Ile Gln Gly Pro Ile Gln Gly Asp Leu Glu Glu Cys Lys Ala Gly Ser
                355                 360                 365
Ala Gly Val Ile Thr Asn Asn Thr Trp Gly Ser Gly Cys Ala Phe Val
            370                 375                 380
Lys Glu Thr Leu Asn Ser Leu Glu Gln His Thr Ala Tyr Tyr Gly Asn
        385                 390                 395
Gln Val Asn Gln Asp Arg Ala Leu Ala Gln Thr Ile Leu Asn Phe Lys
    400                 405                 410
Glu Ala Leu Asn Thr Leu Asn Lys Asp Ser Lys Ala Ile Asn Ser Gly
415                 420                 425                 430
Ile Ser Asn Leu Pro Asn Ala Lys Ser Leu Gln Asn Met Thr His Ala
                435                 440                 445
Thr Gln Asn Pro Asn Ser Pro Glu Gly Leu Leu Thr Tyr Ser Leu Asp
            450                 455                 460
Ser Ser Lys Tyr Asn Gln Leu Gln Thr Ile Ala Gln Glu Leu Gly Lys
        465                 470                 475
Asn Pro Phe Arg Arg Phe Gly Val Ile Asp Phe Gln Asn Asn Asn Gly
    480                 485                 490
Ala Met Asn Gly Ile Gly Val Gln Val Gly Tyr Lys Gln Phe Phe Gly
495                 500                 505                 510
Lys Lys Arg Asn Trp Gly Leu Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn
                515                 520                 525
His Ala Tyr Ile Lys Ser Asn Phe Phe Asn Ser Ala Ser Asp Val Trp
            530                 535                 540
Thr Tyr Gly Val Gly Met Asp Ala Leu Tyr Asn Phe Ile Asn Asp Lys
        545                 550                 555
Asn Thr Asn Phe Leu Gly Lys Asn Asn Lys Leu Ser Val Gly Leu Phe
    560                 565                 570
Gly Gly Phe Ala Leu Ala Gly Thr Ser Trp Leu Asn Ser Gln Gln Val
575                 580                 585                 590
Asn Leu Thr Met Met Asn Gly Ile Tyr Asn Ala Asn Val Ser Thr Ser
                595                 600                 605
Asn Phe Gln Phe Leu Phe Asp Leu Gly Leu Arg Met Asn Leu Ala Arg
            610                 615                 620
Pro Lys Lys Lys Asp Ser Asp His Ala Ala Gln His Gly Ile Glu Leu
        625                 630                 635
Gly Phe Lys Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Phe Met Gly
    640                 645                 650
Ala Lys Leu Glu Tyr Arg Arg Met Tyr Ser Leu Phe Leu Asn Tyr Val
655                 660                 665                 670
Phe Ala Tyr
 
           
             13 
             2270 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (130)...(2049) 
             
             
               sig_peptide 
               (130)...(193) 
             
           
            13
attgagcgca tcaaaacacc ctaaaacttt tttgaaatcc aataaattta tgttataatt     60
aaacgcattg taaataaatt ctcattttga tacattttta caataaaaca ttactttaag    120
gaacatctt atg aaa aaa acg aaa aaa acg att ctg ctt tct cta act ctc    171
          Met Lys Lys Thr Lys Lys Thr Ile Leu Leu Ser Leu Thr Leu
              -20                 -15                 -10
gcg gcg tca ttg ctc cat gct gaa gac aac ggc gtt ttt tta agc gtg      219
Ala Ala Ser Leu Leu His Ala Glu Asp Asn Gly Val Phe Leu Ser Val
         -5                 1               5
ggt tat caa atc ggt gaa gcg gtt caa aaa gtg aaa aac gcc gac aag      267
Gly Tyr Gln Ile Gly Glu Ala Val Gln Lys Val Lys Asn Ala Asp Lys
 10                  15                  20                  25
gtg caa aaa ctt tca gac act tat gaa caa tta agc cgg ctt tta acc      315
Val Gln Lys Leu Ser Asp Thr Tyr Glu Gln Leu Ser Arg Leu Leu Thr
                 30                  35                  40
aac gat aat ggc aca aac tca aag aca agc gcg caa atc aac caa gcg      363
Asn Asp Asn Gly Thr Asn Ser Lys Thr Ser Ala Gln Ile Asn Gln Ala
             45                  50                  55
gtt aat aat ttg aac gaa cgc gca aaa act tta gcc ggt ggg aca acc      411
Val Asn Asn Leu Asn Glu Arg Ala Lys Thr Leu Ala Gly Gly Thr Thr
         60                  65                  70
aat tcc cct gcc tat caa gcc acg ctt tta gcg ttg aga tcg gtg tta      459
Asn Ser Pro Ala Tyr Gln Ala Thr Leu Leu Ala Leu Arg Ser Val Leu
     75                  80                  85
ggg cta tgg aat agc atg ggt tat gcg gtc ata tgc gga ggt tat acc      507
Gly Leu Trp Asn Ser Met Gly Tyr Ala Val Ile Cys Gly Gly Tyr Thr
 90                  95                 100                 105
aaa agt cca ggc gaa aac aat caa aaa gat ttc cac tac acc gat gag      555
Lys Ser Pro Gly Glu Asn Asn Gln Lys Asp Phe His Tyr Thr Asp Glu
                110                 115                 120
aat ggc aat ggc act aca atc aat tgc ggt ggg agc aca aat agt aat      603
Asn Gly Asn Gly Thr Thr Ile Asn Cys Gly Gly Ser Thr Asn Ser Asn
            125                 130                 135
ggc act cat agt tct agt ggc aca aat aca tta aaa gca gac aaa aat      651
Gly Thr His Ser Ser Ser Gly Thr Asn Thr Leu Lys Ala Asp Lys Asn
        140                 145                 150
gtt tct cta tct att gag caa tat gaa aaa atc cat gaa gct tat cag      699
Val Ser Leu Ser Ile Glu Gln Tyr Glu Lys Ile His Glu Ala Tyr Gln
    155                 160                 165
att ctt tca aaa gct tta aaa caa gcc ggg ctt gct cct tta aat agc      747
Ile Leu Ser Lys Ala Leu Lys Gln Ala Gly Leu Ala Pro Leu Asn Ser
170                 175                 180                 185
aaa ggg gaa aag tta gaa gcg cat gta acc aca tca aaa cca gaa aat      795
Lys Gly Glu Lys Leu Glu Ala His Val Thr Thr Ser Lys Pro Glu Asn
                190                 195                 200
aat agt caa act aaa acg aca act tct gtt att gat acg act aat gat      843
Asn Ser Gln Thr Lys Thr Thr Thr Ser Val Ile Asp Thr Thr Asn Asp
            205                 210                 215
gcg caa aat ctt ttg act caa gcg caa acg att gtc aat acc ctt aaa      891
Ala Gln Asn Leu Leu Thr Gln Ala Gln Thr Ile Val Asn Thr Leu Lys
        220                 225                 230
gat tat tgc ccc atg ttg ata gcg aaa tct agt agt gaa agt agt ggc      939
Asp Tyr Cys Pro Met Leu Ile Ala Lys Ser Ser Ser Glu Ser Ser Gly
    235                 240                 245
gca gct act aca aac gcc cct tca tgg caa aca gcc ggt ggc ggc aaa      987
Ala Ala Thr Thr Asn Ala Pro Ser Trp Gln Thr Ala Gly Gly Gly Lys
250                 255                 260                 265
aat tca tgt gcg act ttt ggt gcg gag ttt agt gcc gct tca gac atg     1035
Asn Ser Cys Ala Thr Phe Gly Ala Glu Phe Ser Ala Ala Ser Asp Met
                270                 275                 280
att aat aat gcg caa aaa atc gtt caa gaa acc caa caa ctc agc gcc     1083
Ile Asn Asn Ala Gln Lys Ile Val Gln Glu Thr Gln Gln Leu Ser Ala
            285                 290                 295
aac caa cca aaa aat atc aca caa ccc cat aat ctc aac ctt aac acc     1131
Asn Gln Pro Lys Asn Ile Thr Gln Pro His Asn Leu Asn Leu Asn Thr
        300                 305                 310
cct agc agt ctt acg gct tta gct caa aaa atg ctc aaa aat gcg caa     1179
Pro Ser Ser Leu Thr Ala Leu Ala Gln Lys Met Leu Lys Asn Ala Gln
    315                 320                 325
tct caa gca gaa att tta aaa cta gcc aat caa gtg gag agc gat ttt     1227
Ser Gln Ala Glu Ile Leu Lys Leu Ala Asn Gln Val Glu Ser Asp Phe
330                 335                 340                 345
aac aaa ctt tct tca ggc cat ctt aaa gac tac ata ggg aaa tgc gat     1275
Asn Lys Leu Ser Ser Gly His Leu Lys Asp Tyr Ile Gly Lys Cys Asp
                350                 355                 360
gcg agc gct ata agc agt gcg aat atg aca atg caa aat caa aag aac     1323
Ala Ser Ala Ile Ser Ser Ala Asn Met Thr Met Gln Asn Gln Lys Asn
            365                 370                 375
aat tgg ggg aac ggg tgt gct ggc gtg gaa gaa act ctg tct tca tta     1371
Asn Trp Gly Asn Gly Cys Ala Gly Val Glu Glu Thr Leu Ser Ser Leu
        380                 385                 390
aaa aca agt gcc gct gat ttt aac aac caa acg cca caa atc aat caa     1419
Lys Thr Ser Ala Ala Asp Phe Asn Asn Gln Thr Pro Gln Ile Asn Gln
    395                 400                 405
gcg caa aac cta gcc aac acc ctt att caa gaa ctt ggc aac aac cct     1467
Ala Gln Asn Leu Ala Asn Thr Leu Ile Gln Glu Leu Gly Asn Asn Pro
410                 415                 420                 425
ttt agg aat atg ggc atg atc gct tct tca acc acg aat aac ggc gcc     1515
Phe Arg Asn Met Gly Met Ile Ala Ser Ser Thr Thr Asn Asn Gly Ala
                430                 435                 440
ttg aat ggc ctt ggg gtg caa gtg ggt tat aag caa ttt ttt ggg gaa     1563
Leu Asn Gly Leu Gly Val Gln Val Gly Tyr Lys Gln Phe Phe Gly Glu
            445                 450                 455
aag aaa aga tgg ggg tta agg tat tat ggt ttc ttt gat tac aac cac     1611
Lys Lys Arg Trp Gly Leu Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn His
        460                 465                 470
gcc tat atc aaa tcc aat ttc ttt aac tcg gct tct gat gtg tgg act     1659
Ala Tyr Ile Lys Ser Asn Phe Phe Asn Ser Ala Ser Asp Val Trp Thr
    475                 480                 485
tat ggg gtg ggc agc gat tta ttg ttt aat ttc atc aat gat aaa aac     1707
Tyr Gly Val Gly Ser Asp Leu Leu Phe Asn Phe Ile Asn Asp Lys Asn
490                 495                 500                 505
acc aac ttt tta ggc aag aat aac aag att tca gtg gga ttt ttt gga     1755
Thr Asn Phe Leu Gly Lys Asn Asn Lys Ile Ser Val Gly Phe Phe Gly
                510                 515                 520
ggt atc gcc tta gca ggg act tca tgg ctt aat tct caa ttc gtg aat     1803
Gly Ile Ala Leu Ala Gly Thr Ser Trp Leu Asn Ser Gln Phe Val Asn
            525                 530                 535
tta aaa acc atc agc aat gtt tat agc gct aaa gtg aat acg gct aac     1851
Leu Lys Thr Ile Ser Asn Val Tyr Ser Ala Lys Val Asn Thr Ala Asn
        540                 545                 550
ttc caa ttt tta ttc aat ttg ggc ttg aga acc aat ctc gct aga cct     1899
Phe Gln Phe Leu Phe Asn Leu Gly Leu Arg Thr Asn Leu Ala Arg Pro
    555                 560                 565
aag aaa aaa gat agt cat cat gcg gct caa cat ggc atg gaa ttg ggc     1947
Lys Lys Lys Asp Ser His His Ala Ala Gln His Gly Met Glu Leu Gly
570                 575                 580                 585
gtg aaa atc cct acc att aac acg aat tat tat tct ttt cta gac act     1995
Val Lys Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Phe Leu Asp Thr
                590                 595                 600
aaa cta gaa tat cga agg ctt tat agc gtg tat ctc aat tat gtg ttt     2043
Lys Leu Glu Tyr Arg Arg Leu Tyr Ser Val Tyr Leu Asn Tyr Val Phe
            605                 610                 615
gcc tat taaaaaccct ctttttaaaa aagggggggc tttaaaaaac ctctaaagat      2099
Ala Tyr
aaaaattttc aaaaaacaat cattaaaccc taaaaaagaa attttaaggt ataatgcttt   2159
cgccattttt aattttccat ggcaaactcc tttttagaat ttatccccat aatcgctctt   2219
atggggcgtt tgttttgcaa caatcttttc gaaactatcc aacaagcttt a            2270
 
           
             14 
             640 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(21) 
             
           
            14
Met Lys Lys Thr Lys Lys Thr Ile Leu Leu Ser Leu Thr Leu Ala Ala
    -20                 -15                 -10
Ser Leu Leu His Ala Glu Asp Asn Gly Val Phe Leu Ser Val Gly Tyr
-5                   1               5                  10
Gln Ile Gly Glu Ala Val Gln Lys Val Lys Asn Ala Asp Lys Val Gln
            15                  20                  25
Lys Leu Ser Asp Thr Tyr Glu Gln Leu Ser Arg Leu Leu Thr Asn Asp
        30                  35                  40
Asn Gly Thr Asn Ser Lys Thr Ser Ala Gln Ile Asn Gln Ala Val Asn
    45                  50                  55
Asn Leu Asn Glu Arg Ala Lys Thr Leu Ala Gly Gly Thr Thr Asn Ser
60                  65                  70                  75
Pro Ala Tyr Gln Ala Thr Leu Leu Ala Leu Arg Ser Val Leu Gly Leu
                80                  85                  90
Trp Asn Ser Met Gly Tyr Ala Val Ile Cys Gly Gly Tyr Thr Lys Ser
            95                  100                 105
Pro Gly Glu Asn Asn Gln Lys Asp Phe His Tyr Thr Asp Glu Asn Gly
        110                 115                 120
Asn Gly Thr Thr Ile Asn Cys Gly Gly Ser Thr Asn Ser Asn Gly Thr
    125                 130                 135
His Ser Ser Ser Gly Thr Asn Thr Leu Lys Ala Asp Lys Asn Val Ser
140                 145                 150                 155
Leu Ser Ile Glu Gln Tyr Glu Lys Ile His Glu Ala Tyr Gln Ile Leu
                160                 165                 170
Ser Lys Ala Leu Lys Gln Ala Gly Leu Ala Pro Leu Asn Ser Lys Gly
            175                 180                 185
Glu Lys Leu Glu Ala His Val Thr Thr Ser Lys Pro Glu Asn Asn Ser
        190                 195                 200
Gln Thr Lys Thr Thr Thr Ser Val Ile Asp Thr Thr Asn Asp Ala Gln
    205                 210                 215
Asn Leu Leu Thr Gln Ala Gln Thr Ile Val Asn Thr Leu Lys Asp Tyr
220                 225                 230                 235
Cys Pro Met Leu Ile Ala Lys Ser Ser Ser Glu Ser Ser Gly Ala Ala
                240                 245                 250
Thr Thr Asn Ala Pro Ser Trp Gln Thr Ala Gly Gly Gly Lys Asn Ser
            255                 260                 265
Cys Ala Thr Phe Gly Ala Glu Phe Ser Ala Ala Ser Asp Met Ile Asn
        270                 275                 280
Asn Ala Gln Lys Ile Val Gln Glu Thr Gln Gln Leu Ser Ala Asn Gln
    285                 290                 295
Pro Lys Asn Ile Thr Gln Pro His Asn Leu Asn Leu Asn Thr Pro Ser
300                 305                 310                 315
Ser Leu Thr Ala Leu Ala Gln Lys Met Leu Lys Asn Ala Gln Ser Gln
                320                 325                 330
Ala Glu Ile Leu Lys Leu Ala Asn Gln Val Glu Ser Asp Phe Asn Lys
            335                 340                 345
Leu Ser Ser Gly His Leu Lys Asp Tyr Ile Gly Lys Cys Asp Ala Ser
        350                 355                 360
Ala Ile Ser Ser Ala Asn Met Thr Met Gln Asn Gln Lys Asn Asn Trp
    365                 370                 375
Gly Asn Gly Cys Ala Gly Val Glu Glu Thr Leu Ser Ser Leu Lys Thr
380                 385                 390                 395
Ser Ala Ala Asp Phe Asn Asn Gln Thr Pro Gln Ile Asn Gln Ala Gln
                400                 405                 410
Asn Leu Ala Asn Thr Leu Ile Gln Glu Leu Gly Asn Asn Pro Phe Arg
            415                 420                 425
Asn Met Gly Met Ile Ala Ser Ser Thr Thr Asn Asn Gly Ala Leu Asn
        430                 435                 440
Gly Leu Gly Val Gln Val Gly Tyr Lys Gln Phe Phe Gly Glu Lys Lys
    445                 450                 455
Arg Trp Gly Leu Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn His Ala Tyr
460                 465                 470                 475
Ile Lys Ser Asn Phe Phe Asn Ser Ala Ser Asp Val Trp Thr Tyr Gly
                480                 485                 490
Val Gly Ser Asp Leu Leu Phe Asn Phe Ile Asn Asp Lys Asn Thr Asn
            495                 500                 505
Phe Leu Gly Lys Asn Asn Lys Ile Ser Val Gly Phe Phe Gly Gly Ile
        510                 515                 520
Ala Leu Ala Gly Thr Ser Trp Leu Asn Ser Gln Phe Val Asn Leu Lys
    525                 530                 535
Thr Ile Ser Asn Val Tyr Ser Ala Lys Val Asn Thr Ala Asn Phe Gln
540                 545                 550                 555
Phe Leu Phe Asn Leu Gly Leu Arg Thr Asn Leu Ala Arg Pro Lys Lys
                560                 565                 570
Lys Asp Ser His His Ala Ala Gln His Gly Met Glu Leu Gly Val Lys
            575                 580                 585
Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Phe Leu Asp Thr Lys Leu
        590                 595                 600
Glu Tyr Arg Arg Leu Tyr Ser Val Tyr Leu Asn Tyr Val Phe Ala Tyr
    605                 610                 615
 
           
             15 
             2248 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (173)...(2128) 
             
             
               sig_peptide 
               (173)...(224) 
             
           
            15
tggttttatc gttacaaaat tcaacatttc aaagataaat aagttaaaat accccaaaat     60
cttttttttt tttttgaaat ccaatcaatt tatagtaaaa ttaggttcat tgtaaatata    120
ttatcacttc atgatattct tacaacaaaa acattacttt aaggaacatt tt atg aaa    178
                                                          Met Lys
aag aca att ctg ctc tct ctc tct gct tca tcg ctc ttg cac gct gaa      226
Lys Thr Ile Leu Leu Ser Leu Ser Ala Ser Ser Leu Leu His Ala Glu
 -15                 -10                  -5                 1
gac aac ggc ttt ttt gtg agc gcc ggc tat caa atc ggc gaa gcg gtg      274
Asp Asn Gly Phe Phe Val Ser Ala Gly Tyr Gln Ile Gly Glu Ala Val
             5                   10                  15
caa atg gtc aaa aac acc ggt gaa ttg aaa aac ttg aac gaa aaa tac      322
Gln Met Val Lys Asn Thr Gly Glu Leu Lys Asn Leu Asn Glu Lys Tyr
         20                  25                  30
gag caa tta agc cag tat tta aat caa gtg gct tcg ttg aag caa agc      370
Glu Gln Leu Ser Gln Tyr Leu Asn Gln Val Ala Ser Leu Lys Gln Ser
     35                  40                  45
att caa aac gcc aac aac att gag ctg gtc aat agc tct tta aac tat      418
Ile Gln Asn Ala Asn Asn Ile Glu Leu Val Asn Ser Ser Leu Asn Tyr
 50                  55                  60                  65
tta aaa agc ttt acc aac aac aac tat aac agc acc acc caa tcg ccc      466
Leu Lys Ser Phe Thr Asn Asn Asn Tyr Asn Ser Thr Thr Gln Ser Pro
                 70                  75                  80
atc ttt aat gcc gtg caa gcc gtt atc act tcg gta ttg ggt ttt tgg      514
Ile Phe Asn Ala Val Gln Ala Val Ile Thr Ser Val Leu Gly Phe Trp
             85                  90                  95
agt ctt tat gcg ggg aat tac ttc act ttt ttt gtg ggt aaa aag gtg      562
Ser Leu Tyr Ala Gly Asn Tyr Phe Thr Phe Phe Val Gly Lys Lys Val
        100                 105                 110
ggt gat agt ggg caa ccc gct agt gtc cag ggt aac cct cct ttt aaa      610
Gly Asp Ser Gly Gln Pro Ala Ser Val Gln Gly Asn Pro Pro Phe Lys
    115                 120                 125
acg att ata gag aac tgc tca gga att gaa aac tgc gct atg gat caa      658
Thr Ile Ile Glu Asn Cys Ser Gly Ile Glu Asn Cys Ala Met Asp Gln
130                 135                 140                 145
acc act tat gat aag atg aaa aaa ctc gct gaa gac ctc caa gcg gct      706
Thr Thr Tyr Asp Lys Met Lys Lys Leu Ala Glu Asp Leu Gln Ala Ala
                150                 155                 160
caa aca aac tct gcc act aaa ggc aac aat ctt tgc gct tta tcc ggg      754
Gln Thr Asn Ser Ala Thr Lys Gly Asn Asn Leu Cys Ala Leu Ser Gly
            165                 170                 175
tgt gct gca aca gac tca aca tca aac cca cca aac tca acc gtg agc      802
Cys Ala Ala Thr Asp Ser Thr Ser Asn Pro Pro Asn Ser Thr Val Ser
        180                 185                 190
aac gct ctt aat ttg gcg caa cag ctt atg gat tta atc gca aac act      850
Asn Ala Leu Asn Leu Ala Gln Gln Leu Met Asp Leu Ile Ala Asn Thr
    195                 200                 205
aaa acg gct atg atg tgg aaa aat atc gtc atc agt ggc gtt tca aac      898
Lys Thr Ala Met Met Trp Lys Asn Ile Val Ile Ser Gly Val Ser Asn
210                 215                 220                 225
aca tcc ggt gct atc aca tcc act aat tac cca acg caa tac gcg gtg      946
Thr Ser Gly Ala Ile Thr Ser Thr Asn Tyr Pro Thr Gln Tyr Ala Val
                230                 235                 240
ttt aac aac att aag gcg atg ata ccc att ttg caa caa gcg gtt acg      994
Phe Asn Asn Ile Lys Ala Met Ile Pro Ile Leu Gln Gln Ala Val Thr
            245                 250                 255
ctt tct caa agc aac cac acc cta tct gct agc ttg caa gct caa gcc     1042
Leu Ser Gln Ser Asn His Thr Leu Ser Ala Ser Leu Gln Ala Gln Ala
        260                 265                 270
aca gga tct caa aca aac cct aaa ttc gct aaa gac atc tac act ttc     1090
Thr Gly Ser Gln Thr Asn Pro Lys Phe Ala Lys Asp Ile Tyr Thr Phe
    275                 280                 285
gct caa aac caa aag caa gtc atc tct tac gct caa gac att ttc aac     1138
Ala Gln Asn Gln Lys Gln Val Ile Ser Tyr Ala Gln Asp Ile Phe Asn
290                 295                 300                 305
ctc ttt aat tct atc cct gca gag cag tat aag tat cta gag aaa gct     1186
Leu Phe Asn Ser Ile Pro Ala Glu Gln Tyr Lys Tyr Leu Glu Lys Ala
                310                 315                 320
tac ttg aaa ata ccc aat gcg ggt tca acg cct act aac cct tac aga     1234
Tyr Leu Lys Ile Pro Asn Ala Gly Ser Thr Pro Thr Asn Pro Tyr Arg
            325                 330                 335
caa gtg gtg aat tta aac caa gaa gtt cag acg att aaa aac aat gtg     1282
Gln Val Val Asn Leu Asn Gln Glu Val Gln Thr Ile Lys Asn Asn Val
        340                 345                 350
agt tat tat ggt aac cgg gtg gat gcg gct tta agc gtg gct aga gat     1330
Ser Tyr Tyr Gly Asn Arg Val Asp Ala Ala Leu Ser Val Ala Arg Asp
    355                 360                 365
gtt tat aac cta aaa tcc aat caa gca gaa atc gta acc gcc tat aac     1378
Val Tyr Asn Leu Lys Ser Asn Gln Ala Glu Ile Val Thr Ala Tyr Asn
370                 375                 380                 385
gac gct aag act ttg agc gaa gag att tct aaa ctc ccg cac aat caa     1426
Asp Ala Lys Thr Leu Ser Glu Glu Ile Ser Lys Leu Pro His Asn Gln
                390                 395                 400
gtc aat aca aaa gac att gtt aca cta cct tac gat aaa aac gcc cca     1474
Val Asn Thr Lys Asp Ile Val Thr Leu Pro Tyr Asp Lys Asn Ala Pro
            405                 410                 415
gca gca ggc caa tcc aac tac caa atc aac cca gag cag caa tcc aat     1522
Ala Ala Gly Gln Ser Asn Tyr Gln Ile Asn Pro Glu Gln Gln Ser Asn
        420                 425                 430
ctt aac caa gct tta gca gcg atg agc aat aac ccc ttt aaa aaa gtg     1570
Leu Asn Gln Ala Leu Ala Ala Met Ser Asn Asn Pro Phe Lys Lys Val
    435                 440                 445
ggc atg atc agc tct caa aac aat aac ggc gct ttg aac ggg ctt ggc     1618
Gly Met Ile Ser Ser Gln Asn Asn Asn Gly Ala Leu Asn Gly Leu Gly
450                 455                 460                 465
gtg caa gtg ggt tat aag caa ttc ttt ggc gaa agc aaa aga tgg ggg     1666
Val Gln Val Gly Tyr Lys Gln Phe Phe Gly Glu Ser Lys Arg Trp Gly
                470                 475                 480
tta agg tat tac gga ttc ttt gat tac aac cac ggc tac atc aaa tcc     1714
Leu Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn His Gly Tyr Ile Lys Ser
            485                 490                 495
agc ttc ttt aac tct tct tct gat ata tgg act tat ggc ggt ggg agc     1762
Ser Phe Phe Asn Ser Ser Ser Asp Ile Trp Thr Tyr Gly Gly Gly Ser
        500                 505                 510
gat ttg tta gtg aat att atc aac gat agc atc aca aga aag aac aac     1810
Asp Leu Leu Val Asn Ile Ile Asn Asp Ser Ile Thr Arg Lys Asn Asn
    515                 520                 525
aag ctc tcc gtg ggt ctt ttt gga ggc atc caa cta gca ggg act aca     1858
Lys Leu Ser Val Gly Leu Phe Gly Gly Ile Gln Leu Ala Gly Thr Thr
530                 535                 540                 545
tgg ctt aat tct caa tac gtg aat tta acc gcg ttc aat aac cct tac     1906
Trp Leu Asn Ser Gln Tyr Val Asn Leu Thr Ala Phe Asn Asn Pro Tyr
                550                 555                 560
agc gcg aaa gtc aat gct acc aat ttc caa ttc ttg ttc aat ctc ggc     1954
Ser Ala Lys Val Asn Ala Thr Asn Phe Gln Phe Leu Phe Asn Leu Gly
            565                 570                 575
ttg agg acg aat ctc gct aca gct agg aaa aaa gac agc gaa cat tcc     2002
Leu Arg Thr Asn Leu Ala Thr Ala Arg Lys Lys Asp Ser Glu His Ser
        580                 585                 590
gcg caa cat ggc att gaa ttg ggt att aaa atc ccc acc att acc acg     2050
Ala Gln His Gly Ile Glu Leu Gly Ile Lys Ile Pro Thr Ile Thr Thr
    595                 600                 605
aat tac tat tct ttt cta ggc act caa ttg caa tac aga agg ctc tat     2098
Asn Tyr Tyr Ser Phe Leu Gly Thr Gln Leu Gln Tyr Arg Arg Leu Tyr
610                 615                 620                 625
agc gtg tat ctc aat tat gtg ttc gct tac tgagtgattc aagctctctt       2148
Ser Val Tyr Leu Asn Tyr Val Phe Ala Tyr
                630                 635
ctttaagggg gtttagaaaa atcgcaacgc caagcttttt atcgttggtg ataaaatcta   2208
caaaactaac ggcgcgacaa caaaccctaa cgctacgctc                         2248
 
           
             16 
             652 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(17) 
             
           
            16
Met Lys Lys Thr Ile Leu Leu Ser Leu Ser Ala Ser Ser Leu Leu His
        -15                 -10                 -5
Ala Glu Asp Asn Gly Phe Phe Val Ser Ala Gly Tyr Gln Ile Gly Glu
     1               5                  10                  15
Ala Val Gln Met Val Lys Asn Thr Gly Glu Leu Lys Asn Leu Asn Glu
                20                  25                  30
Lys Tyr Glu Gln Leu Ser Gln Tyr Leu Asn Gln Val Ala Ser Leu Lys
            35                  40                  45
Gln Ser Ile Gln Asn Ala Asn Asn Ile Glu Leu Val Asn Ser Ser Leu
        50                  55                  60
Asn Tyr Leu Lys Ser Phe Thr Asn Asn Asn Tyr Asn Ser Thr Thr Gln
    65                  70                  75
Ser Pro Ile Phe Asn Ala Val Gln Ala Val Ile Thr Ser Val Leu Gly
80                  85                  90                  95
Phe Trp Ser Leu Tyr Ala Gly Asn Tyr Phe Thr Phe Phe Val Gly Lys
                100                 105                 110
Lys Val Gly Asp Ser Gly Gln Pro Ala Ser Val Gln Gly Asn Pro Pro
            115                 120                 125
Phe Lys Thr Ile Ile Glu Asn Cys Ser Gly Ile Glu Asn Cys Ala Met
        130                 135                 140
Asp Gln Thr Thr Tyr Asp Lys Met Lys Lys Leu Ala Glu Asp Leu Gln
    145                 150                 155
Ala Ala Gln Thr Asn Ser Ala Thr Lys Gly Asn Asn Leu Cys Ala Leu
160                 165                 170                 175
Ser Gly Cys Ala Ala Thr Asp Ser Thr Ser Asn Pro Pro Asn Ser Thr
                180                 185                 190
Val Ser Asn Ala Leu Asn Leu Ala Gln Gln Leu Met Asp Leu Ile Ala
            195                 200                 205
Asn Thr Lys Thr Ala Met Met Trp Lys Asn Ile Val Ile Ser Gly Val
        210                 215                 220
Ser Asn Thr Ser Gly Ala Ile Thr Ser Thr Asn Tyr Pro Thr Gln Tyr
    225                 230                 235
Ala Val Phe Asn Asn Ile Lys Ala Met Ile Pro Ile Leu Gln Gln Ala
240                 245                 250                 255
Val Thr Leu Ser Gln Ser Asn His Thr Leu Ser Ala Ser Leu Gln Ala
                260                 265                 270
Gln Ala Thr Gly Ser Gln Thr Asn Pro Lys Phe Ala Lys Asp Ile Tyr
            275                 280                 285
Thr Phe Ala Gln Asn Gln Lys Gln Val Ile Ser Tyr Ala Gln Asp Ile
        290                 295                 300
Phe Asn Leu Phe Asn Ser Ile Pro Ala Glu Gln Tyr Lys Tyr Leu Glu
    305                 310                 315
Lys Ala Tyr Leu Lys Ile Pro Asn Ala Gly Ser Thr Pro Thr Asn Pro
320                 325                 330                 335
Tyr Arg Gln Val Val Asn Leu Asn Gln Glu Val Gln Thr Ile Lys Asn
                340                 345                 350
Asn Val Ser Tyr Tyr Gly Asn Arg Val Asp Ala Ala Leu Ser Val Ala
            355                 360                 365
Arg Asp Val Tyr Asn Leu Lys Ser Asn Gln Ala Glu Ile Val Thr Ala
        370                 375                 380
Tyr Asn Asp Ala Lys Thr Leu Ser Glu Glu Ile Ser Lys Leu Pro His
    385                 390                 395
Asn Gln Val Asn Thr Lys Asp Ile Val Thr Leu Pro Tyr Asp Lys Asn
400                 405                 410                 415
Ala Pro Ala Ala Gly Gln Ser Asn Tyr Gln Ile Asn Pro Glu Gln Gln
                420                 425                 430
Ser Asn Leu Asn Gln Ala Leu Ala Ala Met Ser Asn Asn Pro Phe Lys
            435                 440                 445
Lys Val Gly Met Ile Ser Ser Gln Asn Asn Asn Gly Ala Leu Asn Gly
        450                 455                 460
Leu Gly Val Gln Val Gly Tyr Lys Gln Phe Phe Gly Glu Ser Lys Arg
    465                 470                 475
Trp Gly Leu Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn His Gly Tyr Ile
480                 485                 490                 495
Lys Ser Ser Phe Phe Asn Ser Ser Ser Asp Ile Trp Thr Tyr Gly Gly
                500                 505                 510
Gly Ser Asp Leu Leu Val Asn Ile Ile Asn Asp Ser Ile Thr Arg Lys
            515                 520                 525
Asn Asn Lys Leu Ser Val Gly Leu Phe Gly Gly Ile Gln Leu Ala Gly
        530                 535                 540
Thr Thr Trp Leu Asn Ser Gln Tyr Val Asn Leu Thr Ala Phe Asn Asn
    545                 550                 555
Pro Tyr Ser Ala Lys Val Asn Ala Thr Asn Phe Gln Phe Leu Phe Asn
560                 565                 570                 575
Leu Gly Leu Arg Thr Asn Leu Ala Thr Ala Arg Lys Lys Asp Ser Glu
                580                 585                 590
His Ser Ala Gln His Gly Ile Glu Leu Gly Ile Lys Ile Pro Thr Ile
            595                 600                 605
Thr Thr Asn Tyr Tyr Ser Phe Leu Gly Thr Gln Leu Gln Tyr Arg Arg
        610                 615                 620
Leu Tyr Ser Val Tyr Leu Asn Tyr Val Phe Ala Tyr
    625                 630                 635
 
           
             17 
             2161 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (122)...(2056) 
             
             
               sig_peptide 
               (122)...(179) 
             
           
            17
caaaaatctt tttttttttt ttttgaaatc caataaattt atggtaaagt taaacatatt     60
gtaaataaat tttaatttct attcatgttt acaataaaaa aattacttta aggaacattt    120
t atg aaa aag aca att cta ctc tct ctc tct ctc tcg ctt tca tcg ctc    169
  Met Lys Lys Thr Ile Leu Leu Ser Leu Ser Leu Ser Leu Ser Ser Leu
                  -15                 -10                  -5
ttg cac gct gaa gac aac ggc ttt ttt gtg agc gcc ggc tat caa atc      217
Leu His Ala Glu Asp Asn Gly Phe Phe Val Ser Ala Gly Tyr Gln Ile
            1               5                   10
ggc gaa cgg gtg caa atg gtc aaa aac acc ggc gaa ttg aaa aac ttg      265
Gly Glu Arg Val Gln Met Val Lys Asn Thr Gly Glu Leu Lys Asn Leu
     15                  20                  25
aac gaa aaa tac gag caa tta agc caa tct tta gcc caa ctg gct tcg      313
Asn Glu Lys Tyr Glu Gln Leu Ser Gln Ser Leu Ala Gln Leu Ala Ser
 30                  35                  40                  45
tta aaa aaa agc att caa acg gcg aac aac att cag gct gtc aac aat      361
Leu Lys Lys Ser Ile Gln Thr Ala Asn Asn Ile Gln Ala Val Asn Asn
                 50                  55                  60
gct tta agc gat tta aaa agc ttt gcg agt aac aac cac aca aac aaa      409
Ala Leu Ser Asp Leu Lys Ser Phe Ala Ser Asn Asn His Thr Asn Lys
             65                  70                  75
gaa aca tcg ccc atc tac aac acc gcg caa gct gtt atc act tca gta      457
Glu Thr Ser Pro Ile Tyr Asn Thr Ala Gln Ala Val Ile Thr Ser Val
         80                  85                  90
ttg gct ttt tgg agt ctt tat gca ggg aac gct acc agt ttt cat gtg      505
Leu Ala Phe Trp Ser Leu Tyr Ala Gly Asn Ala Thr Ser Phe His Val
     95                 100                 105
acc ggt ttg aat gat gga tct aat gct cct ctt gga aga atc cat caa      553
Thr Gly Leu Asn Asp Gly Ser Asn Ala Pro Leu Gly Arg Ile His Gln
110                 115                 120                 125
gat ggg aac tgc aca gga tta caa caa tgt ttt atg aat aaa gaa act      601
Asp Gly Asn Cys Thr Gly Leu Gln Gln Cys Phe Met Asn Lys Glu Thr
                130                 135                 140
tat gat aaa atg aaa gcg ctt gcc gaa aat ctc caa aaa gct caa ggc      649
Tyr Asp Lys Met Lys Ala Leu Ala Glu Asn Leu Gln Lys Ala Gln Gly
            145                 150                 155
aat ctc tgt gcc tta tca gaa tgc cct agc gat caa tta aat gga aac      697
Asn Leu Cys Ala Leu Ser Glu Cys Pro Ser Asp Gln Leu Asn Gly Asn
        160                 165                 170
aat gga aac aaa act tcc atg act aaa gct ctt gaa acc gcg caa cag      745
Asn Gly Asn Lys Thr Ser Met Thr Lys Ala Leu Glu Thr Ala Gln Gln
    175                 180                 185
ctt atg gat tta atc gca aac act aaa acg gct atg atg tgg aaa aat      793
Leu Met Asp Leu Ile Ala Asn Thr Lys Thr Ala Met Met Trp Lys Asn
190                 195                 200                 205
atc gtc atc gca ggt gtt aca aac aga ccc ggt ggt gct ggc gct atc      841
Ile Val Ile Ala Gly Val Thr Asn Arg Pro Gly Gly Ala Gly Ala Ile
                210                 215                 220
aca tcc act ggt cct gta acc gac tat gcg gtg ttt aac aac att aag      889
Thr Ser Thr Gly Pro Val Thr Asp Tyr Ala Val Phe Asn Asn Ile Lys
            225                 230                 235
gcg atg ata ccc att ttg caa caa gcg gtt acg ctt tct caa agc aac      937
Ala Met Ile Pro Ile Leu Gln Gln Ala Val Thr Leu Ser Gln Ser Asn
        240                 245                 250
cac acc cta tct gct agc ttg caa gct caa gcc aca gga tct caa aca      985
His Thr Leu Ser Ala Ser Leu Gln Ala Gln Ala Thr Gly Ser Gln Thr
    255                 260                 265
aac cct aaa ttc gct aaa gac atc tac act ttc gct caa aac caa aag     1033
Asn Pro Lys Phe Ala Lys Asp Ile Tyr Thr Phe Ala Gln Asn Gln Lys
270                 275                 280                 285
caa gtc atc tct tac gct caa gac att ttc aac ctc ttt aat tct atc     1081
Gln Val Ile Ser Tyr Ala Gln Asp Ile Phe Asn Leu Phe Asn Ser Ile
                290                 295                 300
cct gca gag cag tat aag tat cta gag aaa gct tac ttg aaa ata ccc     1129
Pro Ala Glu Gln Tyr Lys Tyr Leu Glu Lys Ala Tyr Leu Lys Ile Pro
            305                 310                 315
aat gcg ggt tca acg cct act aac cct tac aga caa gtg gtg aat tta     1177
Asn Ala Gly Ser Thr Pro Thr Asn Pro Tyr Arg Gln Val Val Asn Leu
        320                 325                 330
aac caa gaa gtt cag acg att aaa aac aat gtg agt tat tat ggt aac     1225
Asn Gln Glu Val Gln Thr Ile Lys Asn Asn Val Ser Tyr Tyr Gly Asn
    335                 340                 345
cgg gtg gat gcg gct tta agc gtg gct aga gat gtt tat aac cta aaa     1273
Arg Val Asp Ala Ala Leu Ser Val Ala Arg Asp Val Tyr Asn Leu Lys
350                 355                 360                 365
tcc aat caa gca gaa atc gta acc gcc tat aac gac gct aag act ttg     1321
Ser Asn Gln Ala Glu Ile Val Thr Ala Tyr Asn Asp Ala Lys Thr Leu
                370                 375                 380
agc gaa gag att tct aaa ctc ccg cac aat caa gtc aat aca aaa gac     1369
Ser Glu Glu Ile Ser Lys Leu Pro His Asn Gln Val Asn Thr Lys Asp
            385                 390                 395
att gtt aca cta cct tac gat aaa aac gcc cca gca gca ggc caa tcc     1417
Ile Val Thr Leu Pro Tyr Asp Lys Asn Ala Pro Ala Ala Gly Gln Ser
        400                 405                 410
aac tac caa atc aac cca gag cag caa tcc aat ctt aac caa gct tta     1465
Asn Tyr Gln Ile Asn Pro Glu Gln Gln Ser Asn Leu Asn Gln Ala Leu
    415                 420                 425
gca gcg atg agc aat aac ccc ttt aaa aaa gtg ggc atg atc agc tct     1513
Ala Ala Met Ser Asn Asn Pro Phe Lys Lys Val Gly Met Ile Ser Ser
430                 435                 440                 445
caa aac aat aac ggc gct ttg aac ggg ctt ggc gtg caa gtg ggt tat     1561
Gln Asn Asn Asn Gly Ala Leu Asn Gly Leu Gly Val Gln Val Gly Tyr
                450                 455                 460
aag caa ttc ttt ggc gaa agc aaa aga tgg ggg tta agg tat tac gga     1609
Lys Gln Phe Phe Gly Glu Ser Lys Arg Trp Gly Leu Arg Tyr Tyr Gly
            465                 470                 475
ttc ttt gat tac aac cac ggc tac atc aaa tcc agc ttc ttt aac tct     1657
Phe Phe Asp Tyr Asn His Gly Tyr Ile Lys Ser Ser Phe Phe Asn Ser
        480                 485                 490
tct tct gat ata tgg act tat ggc ggt ggg agc gat ttg tta gtg aat     1705
Ser Ser Asp Ile Trp Thr Tyr Gly Gly Gly Ser Asp Leu Leu Val Asn
    495                 500                 505
att atc aac gat agc atc aca aga aag aac aac aag ctc tcc gtg ggt     1753
Ile Ile Asn Asp Ser Ile Thr Arg Lys Asn Asn Lys Leu Ser Val Gly
510                 515                 520                 525
ctt ttt gga ggc atc caa cta gca ggg act aca tgg ctt aat tct caa     1801
Leu Phe Gly Gly Ile Gln Leu Ala Gly Thr Thr Trp Leu Asn Ser Gln
                530                 535                 540
tac gtg aat tta acc gcg ttc aat aac cct tac agc gcg aaa gtc aat     1849
Tyr Val Asn Leu Thr Ala Phe Asn Asn Pro Tyr Ser Ala Lys Val Asn
            545                 550                 555
gct acc aat ttc caa ttc ttg ttc aat ctc ggc ttg agg acg aat ctc     1897
Ala Thr Asn Phe Gln Phe Leu Phe Asn Leu Gly Leu Arg Thr Asn Leu
        560                 565                 570
gct aca gct agg aaa aaa gac agc gaa cat tcc gcg caa cat ggc att     1945
Ala Thr Ala Arg Lys Lys Asp Ser Glu His Ser Ala Gln His Gly Ile
    575                 580                 585
gaa ttg ggt att aaa atc ccc acc att acc acg aat tac tat tct ttt     1993
Glu Leu Gly Ile Lys Ile Pro Thr Ile Thr Thr Asn Tyr Tyr Ser Phe
590                 595                 600                 605
cta ggc act caa ttg caa tac aga agg ctc tat agc gtg tat ctc aat     2041
Leu Gly Thr Gln Leu Gln Tyr Arg Arg Leu Tyr Ser Val Tyr Leu Asn
                610                 615                 620
tat gtg ttc gct tat taaaaaatct tctttttaaa atagggggag cttcatcaaa     2096
Tyr Val Phe Ala Tyr
            625
tctattttga tagttatcaa tatttgatga aaataaagtc aaaaacaaaa taaaccaaat   2156
caccc                                                               2161
 
           
             18 
             645 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(19) 
             
           
            18
Met Lys Lys Thr Ile Leu Leu Ser Leu Ser Leu Ser Leu Ser Ser Leu
                -15                 -10                 -5
Leu His Ala Glu Asp Asn Gly Phe Phe Val Ser Ala Gly Tyr Gln Ile
             1               5                  10
Gly Glu Arg Val Gln Met Val Lys Asn Thr Gly Glu Leu Lys Asn Leu
    15                  20                  25
Asn Glu Lys Tyr Glu Gln Leu Ser Gln Ser Leu Ala Gln Leu Ala Ser
30                  35                  40                  45
Leu Lys Lys Ser Ile Gln Thr Ala Asn Asn Ile Gln Ala Val Asn Asn
                50                  55                  60
Ala Leu Ser Asp Leu Lys Ser Phe Ala Ser Asn Asn His Thr Asn Lys
            65                  70                  75
Glu Thr Ser Pro Ile Tyr Asn Thr Ala Gln Ala Val Ile Thr Ser Val
        80                  85                  90
Leu Ala Phe Trp Ser Leu Tyr Ala Gly Asn Ala Thr Ser Phe His Val
    95                  100                 105
Thr Gly Leu Asn Asp Gly Ser Asn Ala Pro Leu Gly Arg Ile His Gln
110                 115                 120                 125
Asp Gly Asn Cys Thr Gly Leu Gln Gln Cys Phe Met Asn Lys Glu Thr
                130                 135                 140
Tyr Asp Lys Met Lys Ala Leu Ala Glu Asn Leu Gln Lys Ala Gln Gly
            145                 150                 155
Asn Leu Cys Ala Leu Ser Glu Cys Pro Ser Asp Gln Leu Asn Gly Asn
        160                 165                 170
Asn Gly Asn Lys Thr Ser Met Thr Lys Ala Leu Glu Thr Ala Gln Gln
    175                 180                 185
Leu Met Asp Leu Ile Ala Asn Thr Lys Thr Ala Met Met Trp Lys Asn
190                 195                 200                 205
Ile Val Ile Ala Gly Val Thr Asn Arg Pro Gly Gly Ala Gly Ala Ile
                210                 215                 220
Thr Ser Thr Gly Pro Val Thr Asp Tyr Ala Val Phe Asn Asn Ile Lys
            225                 230                 235
Ala Met Ile Pro Ile Leu Gln Gln Ala Val Thr Leu Ser Gln Ser Asn
        240                 245                 250
His Thr Leu Ser Ala Ser Leu Gln Ala Gln Ala Thr Gly Ser Gln Thr
    255                 260                 265
Asn Pro Lys Phe Ala Lys Asp Ile Tyr Thr Phe Ala Gln Asn Gln Lys
270                 275                 280                 285
Gln Val Ile Ser Tyr Ala Gln Asp Ile Phe Asn Leu Phe Asn Ser Ile
                290                 295                 300
Pro Ala Glu Gln Tyr Lys Tyr Leu Glu Lys Ala Tyr Leu Lys Ile Pro
            305                 310                 315
Asn Ala Gly Ser Thr Pro Thr Asn Pro Tyr Arg Gln Val Val Asn Leu
        320                 325                 330
Asn Gln Glu Val Gln Thr Ile Lys Asn Asn Val Ser Tyr Tyr Gly Asn
    335                 340                 345
Arg Val Asp Ala Ala Leu Ser Val Ala Arg Asp Val Tyr Asn Leu Lys
350                 355                 360                 365
Ser Asn Gln Ala Glu Ile Val Thr Ala Tyr Asn Asp Ala Lys Thr Leu
                370                 375                 380
Ser Glu Glu Ile Ser Lys Leu Pro His Asn Gln Val Asn Thr Lys Asp
            385                 390                 395
Ile Val Thr Leu Pro Tyr Asp Lys Asn Ala Pro Ala Ala Gly Gln Ser
        400                 405                 410
Asn Tyr Gln Ile Asn Pro Glu Gln Gln Ser Asn Leu Asn Gln Ala Leu
    415                 420                 425
Ala Ala Met Ser Asn Asn Pro Phe Lys Lys Val Gly Met Ile Ser Ser
430                 435                 440                 445
Gln Asn Asn Asn Gly Ala Leu Asn Gly Leu Gly Val Gln Val Gly Tyr
                450                 455                 460
Lys Gln Phe Phe Gly Glu Ser Lys Arg Trp Gly Leu Arg Tyr Tyr Gly
            465                 470                 475
Phe Phe Asp Tyr Asn His Gly Tyr Ile Lys Ser Ser Phe Phe Asn Ser
        480                 485                 490
Ser Ser Asp Ile Trp Thr Tyr Gly Gly Gly Ser Asp Leu Leu Val Asn
    495                 500                 505
Ile Ile Asn Asp Ser Ile Thr Arg Lys Asn Asn Lys Leu Ser Val Gly
510                 515                 520                 525
Leu Phe Gly Gly Ile Gln Leu Ala Gly Thr Thr Trp Leu Asn Ser Gln
                530                 535                 540
Tyr Val Asn Leu Thr Ala Phe Asn Asn Pro Tyr Ser Ala Lys Val Asn
            545                 550                 555
Ala Thr Asn Phe Gln Phe Leu Phe Asn Leu Gly Leu Arg Thr Asn Leu
        560                 565                 570
Ala Thr Ala Arg Lys Lys Asp Ser Glu His Ser Ala Gln His Gly Ile
    575                 580                 585
Glu Leu Gly Ile Lys Ile Pro Thr Ile Thr Thr Asn Tyr Tyr Ser Phe
590                 595                 600                 605
Leu Gly Thr Gln Leu Gln Tyr Arg Arg Leu Tyr Ser Val Tyr Leu Asn
                610                 615                 620
Tyr Val Phe Ala Tyr
            625
 
           
             19 
             1799 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (185)...(1633) 
             
             
               sig_peptide 
               (185)...(233) 
             
           
            19
tactcaaaac atttttcact atcaaaaacc ttttttttaa atccaaaaaa aaagcaaaat     60
ttcttaattt ttgctcaatt ttattaaaaa ttcaataaat ttatggcaca atttaaactt    120
attgtaaata aagtttcaat ttgatacgat tttacaaaca aaacattact ttaaggaaca    180
tttt atg aaa aaa acg att tta ctt tct ctt atg gtt tca tcg ctc ctc     229
     Met Lys Lys Thr Ile Leu Leu Ser Leu Met Val Ser Ser Leu Leu
         -15                 -10                  -5
gct gaa aat gac ggc gtt ttt atg agc gtg ggc tat caa atc ggc gaa      277
Ala Glu Asn Asp Gly Val Phe Met Ser Val Gly Tyr Gln Ile Gly Glu
    1               5                   10                  15
gcg gtt caa caa gtg aaa aac acc ggc gaa atc caa aaa gtc tcc aac      325
Ala Val Gln Gln Val Lys Asn Thr Gly Glu Ile Gln Lys Val Ser Asn
                 20                  25                  30
gct tac gaa aat ttg aac aat ctt tta acc cgc tat aac gaa ctc aaa      373
Ala Tyr Glu Asn Leu Asn Asn Leu Leu Thr Arg Tyr Asn Glu Leu Lys
             35                  40                  45
caa acg gcc tct aac acc aat tca agt acc gct caa gcg att gat aat      421
Gln Thr Ala Ser Asn Thr Asn Ser Ser Thr Ala Gln Ala Ile Asp Asn
         50                  55                  60
cta aaa gag agc gct agc cga ttg aaa acg acc ccc aat agc gct aat      469
Leu Lys Glu Ser Ala Ser Arg Leu Lys Thr Thr Pro Asn Ser Ala Asn
     65                  70                  75
caa gcc gtg tct tca gcg ctc agc tct gcg gta gcc atg tgg caa gta      517
Gln Ala Val Ser Ser Ala Leu Ser Ser Ala Val Ala Met Trp Gln Val
 80                  85                  90                  95
ata gtc tct aat tta gcc aat aac tcg cta ccc act agt gaa tac aac      565
Ile Val Ser Asn Leu Ala Asn Asn Ser Leu Pro Thr Ser Glu Tyr Asn
                100                 105                 110
aaa atc aat gcg att tct caa tcg ctc caa aac acc cta gaa aat aaa      613
Lys Ile Asn Ala Ile Ser Gln Ser Leu Gln Asn Thr Leu Glu Asn Lys
            115                 120                 125
aac aat gat ctt aaa att gaa aat gac tac gac cat ctt tta act caa      661
Asn Asn Asp Leu Lys Ile Glu Asn Asp Tyr Asp His Leu Leu Thr Gln
        130                 135                 140
gct agc acc att att aat acc ctt caa agc caa tgc cca ggc ata gac      709
Ala Ser Thr Ile Ile Asn Thr Leu Gln Ser Gln Cys Pro Gly Ile Asp
    145                 150                 155
gga ggc aat ggc aaa cca tgg ggc att aat gca agc ggg aac gca tgc      757
Gly Gly Asn Gly Lys Pro Trp Gly Ile Asn Ala Ser Gly Asn Ala Cys
160                 165                 170                 175
aat att ttt ggc aac acc ttt aac gcc atc act agc atg ata gat agc      805
Asn Ile Phe Gly Asn Thr Phe Asn Ala Ile Thr Ser Met Ile Asp Ser
                180                 185                 190
gct aaa aaa gcc gcc gca gat gcc cga aga act gcc cca gaa agt cca      853
Ala Lys Lys Ala Ala Ala Asp Ala Arg Arg Thr Ala Pro Glu Ser Pro
            195                 200                 205
aac caa cca agt gcg ttt aac aac gct gat ttc aat aaa aac ctt aat      901
Asn Gln Pro Ser Ala Phe Asn Asn Ala Asp Phe Asn Lys Asn Leu Asn
        210                 215                 220
caa gtc tca agc gtt att aat gac acg atc tct tac ctc aaa ggg gac      949
Gln Val Ser Ser Val Ile Asn Asp Thr Ile Ser Tyr Leu Lys Gly Asp
    225                 230                 235
aat tta gca acc atc tac aac acc ctt caa aaa acg ccc gat tct aaa      997
Asn Leu Ala Thr Ile Tyr Asn Thr Leu Gln Lys Thr Pro Asp Ser Lys
240                 245                 250                 255
ggg ttt caa agt ttg gtg agc cga tct agc tat agt tat tcc ctc aac     1045
Gly Phe Gln Ser Leu Val Ser Arg Ser Ser Tyr Ser Tyr Ser Leu Asn
                260                 265                 270
gaa acc caa tat tct gaa ttc caa act acc acc aaa gag ttt ggc cat     1093
Glu Thr Gln Tyr Ser Glu Phe Gln Thr Thr Thr Lys Glu Phe Gly His
            275                 280                 285
aac cct ttt aga agc gtg ggt tta atc aac tct caa agc aat aac gga     1141
Asn Pro Phe Arg Ser Val Gly Leu Ile Asn Ser Gln Ser Asn Asn Gly
        290                 295                 300
gcg atg aat ggc gtg ggc gtg caa tta ggc tat aag caa ttc ttt ggg     1189
Ala Met Asn Gly Val Gly Val Gln Leu Gly Tyr Lys Gln Phe Phe Gly
    305                 310                 315
aaa aat aaa ttt ttt ggg atc cgt tat tat gcc ttt ttt gat tac aac     1237
Lys Asn Lys Phe Phe Gly Ile Arg Tyr Tyr Ala Phe Phe Asp Tyr Asn
320                 325                 330                 335
cat gcc tat atc aaa tcc aac ttt ttc aac tcc gct tcc aat gtt ttc     1285
His Ala Tyr Ile Lys Ser Asn Phe Phe Asn Ser Ala Ser Asn Val Phe
                340                 345                 350
act tat ggc gca ggc agt gat ctt tta ttg aat ttc atc aat ggc gga     1333
Thr Tyr Gly Ala Gly Ser Asp Leu Leu Leu Asn Phe Ile Asn Gly Gly
            355                 360                 365
tcc gat aaa aac cgc aaa gtc tct ttt ggc att ttt gga ggc atc gct     1381
Ser Asp Lys Asn Arg Lys Val Ser Phe Gly Ile Phe Gly Gly Ile Ala
        370                 375                 380
cta gca ggc acg aca tgg ctt aat tcc caa ttt atg aat tta aaa acc     1429
Leu Ala Gly Thr Thr Trp Leu Asn Ser Gln Phe Met Asn Leu Lys Thr
    385                 390                 395
acc aat agc gcc tac agc gct aag atc aac aac acc aat ttc caa ttc     1477
Thr Asn Ser Ala Tyr Ser Ala Lys Ile Asn Asn Thr Asn Phe Gln Phe
400                 405                 410                 415
tta ttc aat act ggt tta agg ctt caa ggg att cac cat ggc gtt gaa     1525
Leu Phe Asn Thr Gly Leu Arg Leu Gln Gly Ile His His Gly Val Glu
                420                 425                 430
tta ggc gtg aaa atc ccc acc atc aac acg aat tac tat tct ttc atg     1573
Leu Gly Val Lys Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Phe Met
            435                 440                 445
ggc gct aaa tta gca tac cga aga ctt tat agc gtg tat ttc aat tat     1621
Gly Ala Lys Leu Ala Tyr Arg Arg Leu Tyr Ser Val Tyr Phe Asn Tyr
        450                 455                 460
gtt ttg gcc tat tgatattgaa tcggttctca ttactaatga ggacaaagcc         1673
Val Leu Ala Tyr
    465
aaactttttg gctctcaatg aataacggca tcattttact tgacttttta caaaaaacac   1733
actaaaattt ctttttcttt tttgagcgaa attccagatt agctcagcgg tagagtaggc   1793
ggctgt                                                              1799
 
           
             20 
             483 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(16) 
             
           
            20
Met Lys Lys Thr Ile Leu Leu Ser Leu Met Val Ser Ser Leu Leu Ala
    -15                 -10                 -5
Glu Asn Asp Gly Val Phe Met Ser Val Gly Tyr Gln Ile Gly Glu Ala
 1               5                  10                  15
Val Gln Gln Val Lys Asn Thr Gly Glu Ile Gln Lys Val Ser Asn Ala
            20                  25                  30
Tyr Glu Asn Leu Asn Asn Leu Leu Thr Arg Tyr Asn Glu Leu Lys Gln
        35                  40                  45
Thr Ala Ser Asn Thr Asn Ser Ser Thr Ala Gln Ala Ile Asp Asn Leu
    50                  55                  60
Lys Glu Ser Ala Ser Arg Leu Lys Thr Thr Pro Asn Ser Ala Asn Gln
65                  70                  75                  80
Ala Val Ser Ser Ala Leu Ser Ser Ala Val Ala Met Trp Gln Val Ile
                85                  90                  95
Val Ser Asn Leu Ala Asn Asn Ser Leu Pro Thr Ser Glu Tyr Asn Lys
            100                 105                 110
Ile Asn Ala Ile Ser Gln Ser Leu Gln Asn Thr Leu Glu Asn Lys Asn
        115                 120                 125
Asn Asp Leu Lys Ile Glu Asn Asp Tyr Asp His Leu Leu Thr Gln Ala
    130                 135                 140
Ser Thr Ile Ile Asn Thr Leu Gln Ser Gln Cys Pro Gly Ile Asp Gly
145                 150                 155                 160
Gly Asn Gly Lys Pro Trp Gly Ile Asn Ala Ser Gly Asn Ala Cys Asn
                165                 170                 175
Ile Phe Gly Asn Thr Phe Asn Ala Ile Thr Ser Met Ile Asp Ser Ala
            180                 185                 190
Lys Lys Ala Ala Ala Asp Ala Arg Arg Thr Ala Pro Glu Ser Pro Asn
        195                 200                 205
Gln Pro Ser Ala Phe Asn Asn Ala Asp Phe Asn Lys Asn Leu Asn Gln
    210                 215                 220
Val Ser Ser Val Ile Asn Asp Thr Ile Ser Tyr Leu Lys Gly Asp Asn
225                 230                 235                 240
Leu Ala Thr Ile Tyr Asn Thr Leu Gln Lys Thr Pro Asp Ser Lys Gly
                245                 250                 255
Phe Gln Ser Leu Val Ser Arg Ser Ser Tyr Ser Tyr Ser Leu Asn Glu
            260                 265                 270
Thr Gln Tyr Ser Glu Phe Gln Thr Thr Thr Lys Glu Phe Gly His Asn
        275                 280                 285
Pro Phe Arg Ser Val Gly Leu Ile Asn Ser Gln Ser Asn Asn Gly Ala
    290                 295                 300
Met Asn Gly Val Gly Val Gln Leu Gly Tyr Lys Gln Phe Phe Gly Lys
305                 310                 315                 320
Asn Lys Phe Phe Gly Ile Arg Tyr Tyr Ala Phe Phe Asp Tyr Asn His
                325                 330                 335
Ala Tyr Ile Lys Ser Asn Phe Phe Asn Ser Ala Ser Asn Val Phe Thr
            340                 345                 350
Tyr Gly Ala Gly Ser Asp Leu Leu Leu Asn Phe Ile Asn Gly Gly Ser
        355                 360                 365
Asp Lys Asn Arg Lys Val Ser Phe Gly Ile Phe Gly Gly Ile Ala Leu
    370                 375                 380
Ala Gly Thr Thr Trp Leu Asn Ser Gln Phe Met Asn Leu Lys Thr Thr
385                 390                 395                 400
Asn Ser Ala Tyr Ser Ala Lys Ile Asn Asn Thr Asn Phe Gln Phe Leu
                405                 410                 415
Phe Asn Thr Gly Leu Arg Leu Gln Gly Ile His His Gly Val Glu Leu
            420                 425                 430
Gly Val Lys Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Phe Met Gly
        435                 440                 445
Ala Lys Leu Ala Tyr Arg Arg Leu Tyr Ser Val Tyr Phe Asn Tyr Val
    450                 455                 460
Leu Ala Tyr
465
 
           
             21 
             2338 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (146)...(2218) 
             
             
               sig_peptide 
               (146)...(200) 
             
           
            21
acttaaaatt gttttttttt tttttcaaaa tataaatttt aagccaaaaa taagcatttt     60
atggtaaaat ggcgaacttt cataaacatg actattatgg gaatgtcatg ggaatgtgaa    120
gaaaaatcta ttaaaaggag aaaac atg aaa aaa tcc ctc tta ctc tct ctt      172
                            Met Lys Lys Ser Leu Leu Leu Ser Leu
                                        -15                 -10
tct ctc atc gct tcc tta tca aga gct gaa gat gac gga ttt tat acg      220
Ser Leu Ile Ala Ser Leu Ser Arg Ala Glu Asp Asp Gly Phe Tyr Thr
                 -5                 1               5
agt gtg ggc tat cag atc ggt gaa gcg gtc caa caa gtg aaa aac aca      268
Ser Val Gly Tyr Gln Ile Gly Glu Ala Val Gln Gln Val Lys Asn Thr
         10                  15                  20
gga gca ttg caa aat ctt gca gac aga tac gat aac tta aac aac ctt      316
Gly Ala Leu Gln Asn Leu Ala Asp Arg Tyr Asp Asn Leu Asn Asn Leu
     25                  30                  35
tta aac caa tac aat tat tta aat tcc tta gtc aat tta gcc agc acg      364
Leu Asn Gln Tyr Asn Tyr Leu Asn Ser Leu Val Asn Leu Ala Ser Thr
 40                  45                  50                  55
ccg agc gcg atc acc ggt gcg att gat aat tta agc tca agc gcg att      412
Pro Ser Ala Ile Thr Gly Ala Ile Asp Asn Leu Ser Ser Ser Ala Ile
                 60                  65                  70
aac ctc act agc gcc acc acc act tcc ccc gcc tat caa gct gtg gct      460
Asn Leu Thr Ser Ala Thr Thr Thr Ser Pro Ala Tyr Gln Ala Val Ala
             75                  80                  85
tta gcg ctc aat gcc gct gtg ggc atg tgg caa gtc ata gcc ctt ttt      508
Leu Ala Leu Asn Ala Ala Val Gly Met Trp Gln Val Ile Ala Leu Phe
         90                  95                 100
att ggc tgt ggc cct ggc cct acc aat aat caa agc tat caa tcg ttt      556
Ile Gly Cys Gly Pro Gly Pro Thr Asn Asn Gln Ser Tyr Gln Ser Phe
    105                 110                 115
ggt aac aca cca gcc ctt aat ggg acc acc acc act tgc aat caa gca      604
Gly Asn Thr Pro Ala Leu Asn Gly Thr Thr Thr Thr Cys Asn Gln Ala
120                 125                 130                 135
tat ggg aca ggc cct aat ggc atc cta tct att gat gaa tac caa aaa      652
Tyr Gly Thr Gly Pro Asn Gly Ile Leu Ser Ile Asp Glu Tyr Gln Lys
                140                 145                 150
ctc aac caa gct tat cag atc atc caa acc gct tta aac caa aat caa      700
Leu Asn Gln Ala Tyr Gln Ile Ile Gln Thr Ala Leu Asn Gln Asn Gln
            155                 160                 165
ggg ggt ggg atg cct gcc ttg aat gac acc acc aaa aca ggg gta gtc      748
Gly Gly Gly Met Pro Ala Leu Asn Asp Thr Thr Lys Thr Gly Val Val
        170                 175                 180
aac ata caa caa acc aat tat agg acc acc aca caa aac aat atc ata      796
Asn Ile Gln Gln Thr Asn Tyr Arg Thr Thr Thr Gln Asn Asn Ile Ile
    185                 190                 195
gag cat tat tat aca gag aat ggg aaa gag atc cca gtc tct tat tca      844
Glu His Tyr Tyr Thr Glu Asn Gly Lys Glu Ile Pro Val Ser Tyr Ser
200                 205                 210                 215
ggc gga tca tca ttc tcg cct aca ata caa ttg aca tac cat aat aac      892
Gly Gly Ser Ser Phe Ser Pro Thr Ile Gln Leu Thr Tyr His Asn Asn
                220                 225                 230
gct gaa aac ctt ttg caa caa gcc gcc act atc atg caa gtc ctt att      940
Ala Glu Asn Leu Leu Gln Gln Ala Ala Thr Ile Met Gln Val Leu Ile
            235                 240                 245
act caa aag ccg cat gtg caa acg agc aat ggc ggt aaa gcg tgg ggg      988
Thr Gln Lys Pro His Val Gln Thr Ser Asn Gly Gly Lys Ala Trp Gly
        250                 255                 260
ttg agt tct acg cct ggg aat gtg atg gat att ttt ggt cct tct ttt     1036
Leu Ser Ser Thr Pro Gly Asn Val Met Asp Ile Phe Gly Pro Ser Phe
    265                 270                 275
aac gct att aat gag atg att aaa aac gct caa aca gcc cta gca aaa     1084
Asn Ala Ile Asn Glu Met Ile Lys Asn Ala Gln Thr Ala Leu Ala Lys
280                 285                 290                 295
acc caa cag ctt aac gct aat gaa aac gcc caa atc acg caa ccc aac     1132
Thr Gln Gln Leu Asn Ala Asn Glu Asn Ala Gln Ile Thr Gln Pro Asn
                300                 305                 310
aat ttc aac ccc tac acc tct aaa gac aaa ggg ttc gct caa gaa atg     1180
Asn Phe Asn Pro Tyr Thr Ser Lys Asp Lys Gly Phe Ala Gln Glu Met
            315                 320                 325
ctc aat aga gct gaa gct caa gca gag att tta aat tta gct aag caa     1228
Leu Asn Arg Ala Glu Ala Gln Ala Glu Ile Leu Asn Leu Ala Lys Gln
        330                 335                 340
gta gcg aac aat ttc cac agc att caa ggg cct att caa ggg gat tta     1276
Val Ala Asn Asn Phe His Ser Ile Gln Gly Pro Ile Gln Gly Asp Leu
    345                 350                 355
gaa gaa tgt aaa gca gga tcg gct ggc gtg atc act aat aac act tgg     1324
Glu Glu Cys Lys Ala Gly Ser Ala Gly Val Ile Thr Asn Asn Thr Trp
360                 365                 370                 375
ggt tca ggt tgc gcg ttt gtg aaa gaa act tta aac tct tta gag caa     1372
Gly Ser Gly Cys Ala Phe Val Lys Glu Thr Leu Asn Ser Leu Glu Gln
                380                 385                 390
cac acc gct tat tac ggc aac cag gtc aat cag gat agg gct ttg gct     1420
His Thr Ala Tyr Tyr Gly Asn Gln Val Asn Gln Asp Arg Ala Leu Ala
            395                 400                 405
caa acc att ttg aat ttt aaa gaa gcc ctt aac acc ctg aat aaa gac     1468
Gln Thr Ile Leu Asn Phe Lys Glu Ala Leu Asn Thr Leu Asn Lys Asp
        410                 415                 420
tca aaa gcg atc aat agc ggt atc tcc aac ttg cct aac gct aaa tct     1516
Ser Lys Ala Ile Asn Ser Gly Ile Ser Asn Leu Pro Asn Ala Lys Ser
    425                 430                 435
ctt caa aac atg acg cat gcc act caa aac cct aat tcc cca gaa ggt     1564
Leu Gln Asn Met Thr His Ala Thr Gln Asn Pro Asn Ser Pro Glu Gly
440                 445                 450                 455
ctg ctc act tat tct ttg gat tca agc aaa tac aac cag ctc caa acc     1612
Leu Leu Thr Tyr Ser Leu Asp Ser Ser Lys Tyr Asn Gln Leu Gln Thr
                460                 465                 470
atc gcg caa gaa ttg ggc aaa aac cct ttc agg cgc ttt ggc gtg att     1660
Ile Ala Gln Glu Leu Gly Lys Asn Pro Phe Arg Arg Phe Gly Val Ile
            475                 480                 485
gac ttt caa aac aac aac ggc gca atg aac ggg atc ggc gtg caa gtg     1708
Asp Phe Gln Asn Asn Asn Gly Ala Met Asn Gly Ile Gly Val Gln Val
        490                 495                 500
ggt tat aaa caa ttc ttt ggt aaa aaa agg aat tgg ggg tta agg tat     1756
Gly Tyr Lys Gln Phe Phe Gly Lys Lys Arg Asn Trp Gly Leu Arg Tyr
    505                 510                 515
tat ggt ttc ttt gat tat aac cat gct tat atc aaa tct aat ttt ttc     1804
Tyr Gly Phe Phe Asp Tyr Asn His Ala Tyr Ile Lys Ser Asn Phe Phe
520                 525                 530                 535
aac tcc gct tct gat gtg tgg act tat ggg gtg ggt atg gac gct ctc     1852
Asn Ser Ala Ser Asp Val Trp Thr Tyr Gly Val Gly Met Asp Ala Leu
                540                 545                 550
tat aac ttc atc aac gat aaa aac acc aac ttt tta ggc aag aac aac     1900
Tyr Asn Phe Ile Asn Asp Lys Asn Thr Asn Phe Leu Gly Lys Asn Asn
            555                 560                 565
aag ctt tca gta ggg ctt ttt gga ggc ttt gcg tta gcc ggg act tcg     1948
Lys Leu Ser Val Gly Leu Phe Gly Gly Phe Ala Leu Ala Gly Thr Ser
        570                 575                 580
tgg ctt aat tcc caa caa gtg aat ttg acc atg atg aat ggc att tat     1996
Trp Leu Asn Ser Gln Gln Val Asn Leu Thr Met Met Asn Gly Ile Tyr
    585                 590                 595
aac gct aat gtc agc act tct aac ttc caa ttt ttg ttt gat tta ggc     2044
Asn Ala Asn Val Ser Thr Ser Asn Phe Gln Phe Leu Phe Asp Leu Gly
600                 605                 610                 615
ttg aga atg aac ctc gct agg cct aag aaa aaa gac agc gat cat gcc     2092
Leu Arg Met Asn Leu Ala Arg Pro Lys Lys Lys Asp Ser Asp His Ala
                620                 625                 630
gct cag cat ggc att gaa cta ggt ttt aag atc ccc acg atc aac acc     2140
Ala Gln His Gly Ile Glu Leu Gly Phe Lys Ile Pro Thr Ile Asn Thr
            635                 640                 645
aac tat tat tct ttc atg ggc gct aaa cta gaa tac aga agg atg tat     2188
Asn Tyr Tyr Ser Phe Met Gly Ala Lys Leu Glu Tyr Arg Arg Met Tyr
        650                 655                 660
agc ctt ttt ctc aat tat gtg ttt gct tac taaaaactct ctttaaaaaa       2238
Ser Leu Phe Leu Asn Tyr Val Phe Ala Tyr
    665                 670
ggggtttgtt taaaaacgct taaaagcatt tttaaaatta agcagtaaag agcctagata   2298
atctcttgca accgctctca agcgataaaa ttaaagtgat                         2338
 
           
             22 
             690 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(18) 
             
           
            22
Met Lys Lys Ser Leu Leu Leu Ser Leu Ser Leu Ile Ala Ser Leu Ser
            -15                 -10                 -5
Ala Glu Asp Asp Gly Phe Tyr Thr Ser Val Gly Tyr Gln Ile Gly Glu
         1               5                  10
Ala Val Gln Gln Val Lys Asn Thr Gly Ala Leu Gln Asn Leu Ala Asp
15                  20                  25                  30
Arg Tyr Asp Asn Leu Asn Asn Leu Leu Asn Gln Tyr Asn Tyr Leu Asn
                35                  40                  45
Ser Leu Val Asn Leu Ala Ser Thr Pro Ser Ala Ile Thr Gly Ala Ile
            50                  55                  60
Asp Asn Leu Ser Ser Ser Ala Ile Asn Leu Thr Ser Ala Thr Thr Thr
        65                  70                  75
Ser Pro Ala Tyr Gln Ala Val Ala Leu Ala Leu Asn Ala Ala Val Gly
    80                  85                  90
Met Trp Gln Val Ile Ala Leu Phe Ile Gly Cys Gly Pro Gly Pro Thr
95                  100                 105                 110
Asn Asn Gln Ser Tyr Gln Ser Phe Gly Asn Thr Pro Ala Leu Asn Gly
                115                 120                 125
Thr Thr Thr Thr Cys Asn Gln Ala Tyr Gly Thr Gly Pro Asn Gly Ile
            130                 135                 140
Leu Ser Ile Asp Glu Tyr Gln Lys Leu Asn Gln Ala Tyr Gln Ile Ile
        145                 150                 155
Gln Thr Ala Leu Asn Gln Asn Gln Gly Gly Gly Met Pro Ala Leu Asn
    160                 165                 170
Asp Thr Thr Lys Thr Gly Val Val Asn Ile Gln Gln Thr Asn Tyr Arg
175                 180                 185                 190
Thr Thr Thr Gln Asn Asn Ile Ile Glu His Tyr Tyr Thr Glu Asn Gly
                195                 200                 205
Lys Glu Ile Pro Val Ser Tyr Ser Gly Gly Ser Ser Phe Ser Pro Thr
            210                 215                 220
Ile Gln Leu Thr Tyr His Asn Asn Ala Glu Asn Leu Leu Gln Gln Ala
        225                 230                 235
Ala Thr Ile Met Gln Val Leu Ile Thr Gln Lys Pro His Val Gln Thr
    240                 245                 250
Ser Asn Gly Gly Lys Ala Trp Gly Leu Ser Ser Thr Pro Gly Asn Val
255                 260                 265                 270
Met Asp Ile Phe Gly Pro Ser Phe Asn Ala Ile Asn Glu Met Ile Lys
                275                 280                 285
Asn Ala Gln Thr Ala Leu Ala Lys Thr Gln Gln Leu Asn Ala Asn Glu
            290                 295                 300
Asn Ala Gln Ile Thr Gln Pro Asn Asn Phe Asn Pro Tyr Thr Ser Lys
        305                 310                 315
Asp Lys Gly Phe Ala Gln Glu Met Leu Asn Arg Ala Glu Ala Gln Ala
    320                 325                 330
Glu Ile Leu Asn Leu Ala Lys Gln Val Ala Asn Asn Phe His Ser Ile
335                 340                 345                 350
Gln Gly Pro Ile Gln Gly Asp Leu Glu Glu Cys Lys Ala Gly Ser Ala
                355                 360                 365
Gly Val Ile Thr Asn Asn Thr Trp Gly Ser Gly Cys Ala Phe Val Lys
            370                 375                 380
Glu Thr Leu Asn Ser Leu Glu Gln His Thr Ala Tyr Tyr Gly Asn Gln
        385                 390                 395
Val Asn Gln Asp Arg Ala Leu Ala Gln Thr Ile Leu Asn Phe Lys Glu
    400                 405                 410
Ala Leu Asn Thr Leu Asn Lys Asp Ser Lys Ala Ile Asn Ser Gly Ile
415                 420                 425                 430
Ser Asn Leu Pro Asn Ala Lys Ser Leu Gln Asn Met Thr His Ala Thr
                435                 440                 445
Gln Asn Pro Asn Ser Pro Glu Gly Leu Leu Thr Tyr Ser Leu Asp Ser
            450                 455                 460
Ser Lys Tyr Asn Gln Leu Gln Thr Ile Ala Gln Glu Leu Gly Lys Asn
        465                 470                 475
Pro Phe Arg Arg Phe Gly Val Ile Asp Phe Gln Asn Asn Asn Gly Ala
    480                 485                 490
Met Asn Gly Ile Gly Val Gln Val Gly Tyr Lys Gln Phe Phe Gly Lys
495                 500                 505                 510
Lys Arg Asn Trp Gly Leu Arg Tyr Tyr Gly Phe Phe Asp Tyr Asn His
                515                 520                 525
Ala Tyr Ile Lys Ser Asn Phe Phe Asn Ser Ala Ser Asp Val Trp Thr
            530                 535                 540
Tyr Gly Val Gly Met Asp Ala Leu Tyr Asn Phe Ile Asn Asp Lys Asn
        545                 550                 555
Thr Asn Phe Leu Gly Lys Asn Asn Lys Leu Ser Val Gly Leu Phe Gly
    560                 565                 570
Gly Phe Ala Leu Ala Gly Thr Ser Trp Leu Asn Ser Gln Gln Val Asn
575                 580                 585                 590
Leu Thr Met Met Asn Gly Ile Tyr Asn Ala Asn Val Ser Thr Ser Asn
                595                 600                 605
Phe Gln Phe Leu Phe Asp Leu Gly Leu Arg Met Asn Leu Ala Arg Pro
            610                 615                 620
Lys Lys Lys Asp Ser Asp His Ala Ala Gln His Gly Ile Glu Leu Gly
        625                 630                 635
Phe Lys Ile Pro Thr Ile Asn Thr Asn Tyr Tyr Ser Phe Met Gly Ala
    640                 645                 650
Lys Leu Glu Tyr Arg Arg Met Tyr Ser Leu Phe Leu Asn Tyr Val Phe
655                 660                 665                 670
Ala Tyr
 
           
             23 
             1149 
             DNA 
             Helicobacter pylori 
             
               CDS 
               (106)...(1002) 
             
             
               sig_peptide 
               (106)...(166) 
             
           
            23
ttactcttta atgtgagttt tctgtgtcat gatagctgat tttgttttaa atttgctata     60
atgtgaattt aatgatgaaa attagtttag agtggagaac acaca atg aaa aaa aat    117
                                                  Met Lys Lys Asn
                                                  -20
atc tta aat tta gcg tta gtg ggt gcg ttg agc acg tcg ttt ttg atg      165
Ile Leu Asn Leu Ala Leu Val Gly Ala Leu Ser Thr Ser Phe Leu Met
    -15                 -10                  -5
gct aag ccg gct cat aac gca aat aac gct acg cat aac acg aaa aaa      213
Ala Lys Pro Ala His Asn Ala Asn Asn Ala Thr His Asn Thr Lys Lys
 1               5                   10                  15
acg act gat tct tca gca ggc gtg tta gcg aca gtg gat ggc aga cct      261
Thr Thr Asp Ser Ser Ala Gly Val Leu Ala Thr Val Asp Gly Arg Pro
             20                  25                  30
atc act aaa agc gat ttt gac atg att aag caa cga aat cct aat ttt      309
Ile Thr Lys Ser Asp Phe Asp Met Ile Lys Gln Arg Asn Pro Asn Phe
         35                  40                  45
gat ttt gac aag ctt aaa gag aaa gaa aaa gaa gcc ttg att gat caa      357
Asp Phe Asp Lys Leu Lys Glu Lys Glu Lys Glu Ala Leu Ile Asp Gln
     50                  55                  60
gct att cgc acc gcc ctt gta gaa aat gaa gct aaa acc gag aaa ttg      405
Ala Ile Arg Thr Ala Leu Val Glu Asn Glu Ala Lys Thr Glu Lys Leu
 65                  70                  75                  80
gac agc act cca gaa ttt aaa gcg atg atg gaa gcg gtt aaa aaa cag      453
Asp Ser Thr Pro Glu Phe Lys Ala Met Met Glu Ala Val Lys Lys Gln
                 85                  90                  95
gct tta gtg gaa ttt tgg gct aaa aaa cag gct gaa gaa gtg aaa aaa      501
Ala Leu Val Glu Phe Trp Ala Lys Lys Gln Ala Glu Glu Val Lys Lys
            100                 105                 110
gtc caa atc cca gaa aaa gaa atg caa gat ttt tac aac gct aac aaa      549
Val Gln Ile Pro Glu Lys Glu Met Gln Asp Phe Tyr Asn Ala Asn Lys
        115                 120                 125
gat cag ctt ttt gtc aag caa gaa gcc cat gct agg cat att tta gtg      597
Asp Gln Leu Phe Val Lys Gln Glu Ala His Ala Arg His Ile Leu Val
    130                 135                 140
aaa acc gaa gat gag gct aaa cgg att att tct gag att gac aaa cag      645
Lys Thr Glu Asp Glu Ala Lys Arg Ile Ile Ser Glu Ile Asp Lys Gln
145                 150                 155                 160
cca aag gct aaa aaa gaa gct aaa ttc att gag tta gcc aat cgg gat      693
Pro Lys Ala Lys Lys Glu Ala Lys Phe Ile Glu Leu Ala Asn Arg Asp
                165                 170                 175
acg att gat cct aac agc aag aac gcg caa aat ggc ggt gat ttg ggg      741
Thr Ile Asp Pro Asn Ser Lys Asn Ala Gln Asn Gly Gly Asp Leu Gly
            180                 185                 190
aaa ttc caa aag aac caa atg gct ccg gat ttt tct aaa gcc gct ttc      789
Lys Phe Gln Lys Asn Gln Met Ala Pro Asp Phe Ser Lys Ala Ala Phe
        195                 200                 205
gct tta act cct ggg gat tac act aaa acc cct gtt aaa aca gag ttt      837
Ala Leu Thr Pro Gly Asp Tyr Thr Lys Thr Pro Val Lys Thr Glu Phe
    210                 215                 220
ggt tat cat att atc tat ttg att tct aaa gat agc cct gta act tat      885
Gly Tyr His Ile Ile Tyr Leu Ile Ser Lys Asp Ser Pro Val Thr Tyr
225                 230                 235                 240
act tat gaa cag gct aaa cct acc att aag ggg atg tta caa gaa aag      933
Thr Tyr Glu Gln Ala Lys Pro Thr Ile Lys Gly Met Leu Gln Glu Lys
                245                 250                 255
ctt ttc caa gaa cgc atg aat caa cgc att gag gaa cta aga aag cac      981
Leu Phe Gln Glu Arg Met Asn Gln Arg Ile Glu Glu Leu Arg Lys His
            260                 265                 270
gct aaa att gtt atc aac aag taattgatga ggtgttatca tgttagttaa        1032
Ala Lys Ile Val Ile Asn Lys
        275
aggcaatgaa attttattga aagcccataa agaaggttat ggggtggggg cgtttaattt   1092
cgtgaatttt gaaatgctaa acgctatttt tgaagcagga aatgaggaaa attcccc      1149
 
           
             24 
             299 
             PRT 
             Helicobacter pylori 
             
               SIGNAL 
               (1)...(20) 
             
           
            24
Met Lys Lys Asn Ile Leu Asn Leu Ala Leu Val Gly Ala Leu Ser Thr
-20                 -15                 -10                 -5
Ser Phe Leu Met Ala Lys Pro Ala His Asn Ala Asn Asn Ala Thr His
                 1               5                  10
Asn Thr Lys Lys Thr Thr Asp Ser Ser Ala Gly Val Leu Ala Thr Val
        15                  20                  25
Asp Gly Arg Pro Ile Thr Lys Ser Asp Phe Asp Met Ile Lys Gln Arg
    30                  35                  40
Asn Pro Asn Phe Asp Phe Asp Lys Leu Lys Glu Lys Glu Lys Glu Ala
45                  50                  55                  60
Leu Ile Asp Gln Ala Ile Arg Thr Ala Leu Val Glu Asn Glu Ala Lys
                65                  70                  75
Thr Glu Lys Leu Asp Ser Thr Pro Glu Phe Lys Ala Met Met Glu Ala
            80                  85                  90
Val Lys Lys Gln Ala Leu Val Glu Phe Trp Ala Lys Lys Gln Ala Glu
        95                  100                 105
Glu Val Lys Lys Val Gln Ile Pro Glu Lys Glu Met Gln Asp Phe Tyr
    110                 115                 120
Asn Ala Asn Lys Asp Gln Leu Phe Val Lys Gln Glu Ala His Ala Arg
125                 130                 135                 140
His Ile Leu Val Lys Thr Glu Asp Glu Ala Lys Arg Ile Ile Ser Glu
                145                 150                 155
Ile Asp Lys Gln Pro Lys Ala Lys Lys Glu Ala Lys Phe Ile Glu Leu
            160                 165                 170
Ala Asn Arg Asp Thr Ile Asp Pro Asn Ser Lys Asn Ala Gln Asn Gly
        175                 180                 185
Gly Asp Leu Gly Lys Phe Gln Lys Asn Gln Met Ala Pro Asp Phe Ser
    190                 195                 200
Lys Ala Ala Phe Ala Leu Thr Pro Gly Asp Tyr Thr Lys Thr Pro Val
205                 210                 215                 220
Lys Thr Glu Phe Gly Tyr His Ile Ile Tyr Leu Ile Ser Lys Asp Ser
                225                 230                 235
Pro Val Thr Tyr Thr Tyr Glu Gln Ala Lys Pro Thr Ile Lys Gly Met
            240                 245                 250
Leu Gln Glu Lys Leu Phe Gln Glu Arg Met Asn Gln Arg Ile Glu Glu
        255                 260                 265
Leu Arg Lys His Ala Lys Ile Val Ile Asn Lys
    270                 275