3DNGPol (3DNucleusGolgiPOLarity) Dataset

Official dataset used to develop 3DCellPol, an automated approach for joint detection and pairing of cell organelles, as described in 3DCellPol: Joint Detection and Pairing of Cell Structures to Compute Cell Polarity.

How to cite (Updates will be made upon publication, currently our paper is available here)

@article{,
  title={3DCellPol: Joint Detection and Pairing of Cell Structures to Compute Cell Polarity},
  author={Narotamo, Hemaxi and Franco, Cl{\'a}udio A and Silveira, Margarida},
  journal={},
  year={},
  publisher={}
}

Image Resolution

The dimensions of each voxel are:

x = 0.666μm
y = 0.666μm
z = 0.270μm

Datasets

We provide both the Real Nucleus Golgi Dataset and the Synthetic Nucleus Golgi Dataset.

Image Acquistion Details

All the retinas are from the GNrep mice (Barbacena et al. 2019, genesis). Tamoxifen was injected intraperitoneally (20μg/g) at two different time points at least 3 days before eyes were collected. Stainings were performed according to previously published protocols (Franco etl al., 2013, Development). Green fluorescence protein (GFP) and mCherry were used to label nuclei and Golgi, respectively. For analysis, a tile-scan spanning the retina was acquired on a Zeiss Cell Observer spinning disk confocal microscope, equipped with the Zen software and with a Plan-Apochromat 40×/1.4 Oil DIC M27 objective. Knock-in mice were maintained at the Instituto de Medicina Molecular under standard husbandry conditions and national regulations. This study was conducted in accordance with European Union (EU) regulations and ethical approval was obtained from the Animal Ethics Committee of Instituto de Medicina Molecular (AWB_2015_10_CF_Polaridade).