Patent Application: US-11851302-A

Abstract:
a novel gene , dubbed “ ys68 ”, involved in primitive hematopoiesis was successfully isolated from cdna derived from mouse yolk sacs . in addition , a human gene corresponding to this gene was successfully isolated . expression characteristics of these genes suggested their involvement in primitive hematopoiesis . the proteins of this invention and genes encoding the proteins may be utilized as tools for drug development against diseases , such as hematological disorders .

Description:
the present invention will be described specifically by way of examples below , however this invention is not restricted in any way to these examples . to obtain molecules that are expressed specifically in hemangioblasts , an experiment was carried out in which cdna of an e14 yolk sac was subtracted from the cdna of an e9 yolk sac . poly a rnas were purified from each of the e9 and e14 yolk sacs , respectively ; then pcr - select cdna subtraction kit ( clonetech ) was used for the subtraction . the obtained cdna fragments were subcloned into pgem - t vectors ( promega ), and then , after selecting highly expressed cdnas in e9 yolk sacs by dot blotting , selected cdna were sequenced . the clone # 68 was a novel gene fragment that was not registered in the database . thus , a primer was designed from the sequence of this gene fragment , and using mouse 15 - day embryo marathon - ready cdna ( clonetech ) as a template , a full - length cdna was isolated by the 5 ′- race method . mouse ys68 encodes 1 , 265 amino acids , but is expected to have further upstream sequence . the obtained ys68 did not have a characteristic motif within its amino acid sequence . however , existence of multiple nuclear transport signals was confirmed . consequently , ys68 was anticipated to be a protein that functions in the nucleus . therefore , to confirm the hypothesis , a vector ( pefbose - flag ( nakashima et al ., febs let . 403 : 79 - 82 , 1997 ) that expresses the mouse ys68 protein ( 1265 amino acids ) tagged with flag was transfected to cos7 cells . after 24 hours , the cells were fixed with 4 % formalin , and was treated with 0 . 1 % triton - x 100 . then , this was reacted with anti - flag antibodies , followed by fitc - labeled anti - mouse igg , and was observed through a fluorescence microscope . consequently , expression of ys68 was strong in the nucleus , as expected ( fig1 ). since the cell nucleus is the site where dna transcription occurs , ys68 is anticipated to be a transcription factor involved with dna transcription . human ys68 gene was isolated by 5 ′- race and 3 ′- race by designing a primer based on the genetic sequence of mouse ys68 . more specifically , based on the genetic sequence of mouse ys68 , est fragments that are thought to be ys68 homologues in humans were searched in the est database . primers were designed based on this est fragment , and using human fetal liver marathon - ready cdna ( clonetech ) as a template , the 5 ′ region and the 3 ′ region cdna were isolated by 5 ′- race and 3 ′- race according to the instructed procedure . the isolated cdna nucleotide sequence is described in seq id no : 11 , and the amino acid sequence of the protein encoded by this cdna is described in seq id no : 12 . a comparison of human and mouse ys68 amino acid sequences is shown in fig8 . the expression distribution of ys68 within tissues was analyzed by northern blotting . total rna was prepared from each tissues of embryonic or adult mice using isogen ( wako ). 25 μg / lane of these samples were electrophoresed . after blotting onto a nylon membrane , hybridization was performed with ys68 cdna fragments labeled with 32 p . hybridization was performed in expresshyb solution ( clonetech ) at 68 ° c . for 2 hours ; then , after several washings with 2 × ssc and 0 . 1 % sds at room temperature , followed by several washings with 0 . 1 × ssc and 0 . 1 % sds at 65 ° c ., autoradiography was performed . the expression of ys68 in adult tissue was the strongest in testis , followed those in kidney and lung . observation of ys68 expression in hematopoietic tissues showed that expression was very strong in liver , thymus and spleen that function as hematopoietic tissues during the embryonic stage . however , expression in these tissues rapidly decreased or was absent in those of adult ( fig2 ). further , the expression pattern in tissues known to be involved in primitive hematopoiesis was investigated in detail . the site of hematopoiesis is known to shift during the embryonic stage as described below from previous studies . first , primitive hematopoiesis starts in the yolk sac at e8 , and definitive hematopoiesis begins later in the agm region at e10 . 5 . hematocytes that developed in agm are immediately transported to liver that is formed around e11 . 5 , then differentiate and proliferate at this site until immediately after birth . meanwhile , hematopoiesis begins to take place in thymus and spleen that are formed around e16 . 5 . after birth , the site of hematopoiesis changes to bone marrow . based on these facts , the expression pattern of ys68 in these tissues was analyzed in further detail by rt - pcr . total rna was extracted from each tissue of mouse embryos at each developmental stage , or an adult mouse ; and 1 μg of each total rna was reverse transcribed to cdna using superscript ii preamplification system ( gibco ). this was used as a template and a ys68 - specific primer ( 68 3 : 5 ′- cacccgtgaagaaacaaataggca - 3 ′/ seq id no : 3 , 68 4 : 5 ′- cctttggtacatgagcttctattt - 5 ′/ seq id no : 4 ) or a g3pdh - specific primer was used to perform pcr ( 25 cycles of 94 ° c . for 30 seconds , 62 ° c . for 30 seconds , and 72 ° c . for 30 seconds ). then was electrophoresed on 1 % agarose gel , and the gel was stained with ethidium bromide . expression of ys68 decreased gradually in the yolk sac , as development proceeded ( fig3 ). against expectations , expression of ys68 was low in the agm region at e10 . 5 , when definitive hematopoiesis begins ( fig5 a ). on the other hand , in liver , thymus , and spleen known to function as sites for hematopoiesis in the embryonic stage , expression of ys68 was very high ( fig4 ) and correlated to the period when these tissues function as hematopoietic organs . furthermore , the expression distribution of ys68 in mouse embryo was analyzed by in situ hybridization . a vector constructed by inserting a 545 bp cdna of the 5 ′- region of ys68 ( positions 898 to 1443 ) into pbluescript ii was used as a template to perform in vitro transcription using t7 rna polymerase or t3 rna polymerase ( boeringer mannheim ), and to synthesize sense or antisense 35 s - labeled rna , respectively . the mouse embryo was removed and frozen to produce slices using a cryostat . after immobilization and acetylation with 4 % paraformaldehyde / pbt , hybridization was performed overnight at 55 ° c . with the above - mentioned rna probe . after treating the reaction solution with rnase a , it was washed several times and autoradiography was performed . the expression of ys68 was the strongest in liver at e11 . 5 ( fig6 ). ys68 was mainly strongly expressed in liver and in the developing thymus , and expression was also confirmed in lungs and neural tube at e14 . 5 ( fig7 ). these results indicate that the expression of ys68 is localized in tissues where active hematopoiesis takes place in a period - specific manner , and strongly suggests that ys68 is a molecule involved in primitive hematopoiesis . its expression was low in the e10 . 5 agm region , which is thought to be the site of development for hematopoietic cells . however this may be due to the absolute number of cells involved in hematopoiesis within the entire agm region , which is not so high . in fact , suda et al . revealed that the percentage of hemangioblasts in the agm region at e10 . 5 is 5 % or less using tek as a marker for hemangioblasts ( hamaguchi et al ., blood 93 : 1549 - 1556 , 1999 ). on the other hand , when e10 . 5 agm region is dispersed and cultivated on a dish , the emergence of hematocytes can be confirmed around the 5th day of cultivation ( mukouyama et al ., immunity 8 : 105 - 114 , 1998 ). interestingly , the expression of ys68 had increased in agm derived cells cultivated for 5 days ( fig4 b ). according to these results , the expression of ys68 is expected to rise in cells that have acquired hematopoietic ability , or in immature hematocytes . using primers constructed from the ys68 gene sequence obtained so far , 5 ′- race was performed using the mouse 15 - day embryo marathon - ready cdna and human fetal liver marathon - ready cdna ( clonetech ) as templates , to clone the upstream 5 ′ region of mouse and human ys68 gene . full - length human and mouse cdna sequences were determined by repeating this 5 ′- race protocol . consequently , human and mouse ys68 were anticipated to encode 2 , 266 and 2 , 243 amino acids , respectively ( fig9 ). comparing the human and mouse amino acid sequences , interestingly , the n - terminal region ( human 1 - 1137 , mouse 1 - 1137 ) had a very high homology of 87 %; whereas the homology in the central region ( human 1138 - 1683 , mouse 1138 - 1679 ) was 57 %, and that in the c - terminal region ( human 1684 - 2266 , mouse 1680 - 2243 ) was very low showing a homology of 45 %. in the c - terminal region with low homology , many nuclear transport signals existed . on the other hand , in the n - terminal region with high homology , two wd repeats existed , which repeats are known to be necessary for interaction among proteins . since the homology in this region is very high between humans and mice , this region is anticipated to be important for the function of ys68 . it was expected that ys68 is bound to some protein in vivo because a protein - binding site ( wd repeats ) exists in the n - terminal region of ys68 . therefore , cell lysate was prepared from cultivated cells of embryonic liver and immunoprecipitation with anti - ys68 antibody was performed . then , sds polyacrylamide gel electrophoresis was performed to investigate whether a protein that coprecipitates with ys68 exists . specifically , cultivated mouse liver cells at e14 . 5 were solubilized with lysis buffer ( 0 . 5 % np - 40 , 10 mm tris - hcl ph7 . 6 , 150 mm nacl , 5 mm edta , 2 mm na 3 vo 4 , 1 mm phenylmethylsulfonyl fluoride , and 5 μg / ml aprotinin ). after incubation overnight at 4 ° c . with anti - ys68 antibodies , protein g was added and was further incubated for 1 hour . sds polyacrylamide gel electrophoresis was conducted after immunoprecipitation , and the gel was stained with silver . consequently , existence of multiple molecules that coprecipitate with ys68 within cells of embryonic liver was confirmed ( fig1 ). this suggested that ys68 functions by binding to several types of proteins within the cell . for detailed analysis of the ys68 expression site , the ys68 protein was used to immunize rabbits to produce polyclonal antibodies against ys68 . the protein encoding the 1208 - 1482 amino acid region of mouse ys68 was expressed in e . coli , was purified according to standard procedures , and was used as the antigen in the production of ys68 polyclonal antibodies . immunization was carried out on rabbits ( new zealand white , 2 . 5 kg , female ) using 200 μg antigen for 1 immunization , with an interval of 10 days for 4 immunizations . then upon collection of whole blood , antiserum was obtained . furthermore , an affinity column with immobilized antigens was prepared , and anti - ys68 polyclonal antibodies were purified from the antiserum . using these antibodies , the expression site in the agm region of e11 . 5 embryo was investigated by immunostaining . immunostaining was conducted as follows . first , slices of frozen mouse embryo were prepared using a cryostat ( leica ). this was immobilized with 4 % formaldehyde and was treated with methanol . after treatment with 0 . 3 % aqueous hydrogen peroxide , blocking was carried out with 3 % bsa , then upon reaction with primary antibodies overnight at 4 ° c . and with secondary antibodies ( hrp - labeled anti - rabbit igg ) at room temperature for 1 hour , washing was repeated 3 times with pbs , and visualization was accomplished by the addition of substrate ( metal enhanced dab substrate kit , pierce ). consequently , the hematocytes existing in the endothelium were stained using red blood cell marker ter119 ( used as a control ; fig1 a , b ), whereas , the vascular endothelium was stained specifically using anti - ys68 antibody ( fig1 c , d , and e ). interestingly , ys68 was darkly stained in the hematocytes emerging from the endothelium cells ( fig1 e , arrow ). in addition , strong expression of ys68 was indicated in the vascular endothelium of the umbilical vein ( fig1 f ). in contrast to ter119 , which selectively stained hematocytes in the blood vessel , ys68 expression was stronger in vascular endothelium than in hematocytes in e9 . 5 yolk sacs ( fig1 g and h ). a liver was surgically removed from an embryo ( e14 . 5 ), cut into small pieces with tweezers , and incubated in cell dissociation buffer ( gibco ) at 37 ° c . for 30 minutes . the cells were further treated with 0 . 1 % collagenase at 37 ° c . for 1 hour , and were loosened by pipetting . after washing several times with pbs , the cells were suspended in dmem containing 10 % fcs , and were cultivated on a 10 - cm dish . to investigate the localization of endogenous ys68 within cells , cultured hepatic cells were stained with anti - ys68 antibodies . first , the cells were fixed with 4 % formalin , and then treated with 0 . 1 % triton - x 100 for cell staining . next , cells were reacted with the primary antibodies , and then with secondary antibodies . the cells were visualized in the same manner as in example 5 . consequently , although ys68 has multiple nuclear transport signals , strong expression was found not only in the nucleus , but also around the nucleus , which expression depended on cells ( fig1 ). next , similar analysis for the expression in hematocytes was carried out . ys68 expression in hematocytes separated from embryonic liver was found to have varied strengths of expression depending on the cell type ( fig1 b ). therefore , the group of hematocytes was sorted using cd34 , which is a marker for immature hematocytes , and ys68 expression in cd34 - positive cells was investigated . to collect cd34 - positive cells , embryonic liver ( e14 . 5 ) was incubated in a dissociation buffer at 37 ° c . for 30 minutes , and then the cells were dissociated by pipetting in pbs . after passing through a nylon mesh filter ( falcon ), the cells were suspended in a sample buffer ( 0 . 5 % bsa , 2 mm edta in pbs ). the cells were reacted with biotin labeled anti - cd34 antibodies ( pharmingen ), followed by fitc labeled streptavidin at 4 ° c ., and then were incubated with anti - fitc microbeads . cd34 positive cells were eluted using macs ( magnetic cell sorting ) column according to the instructed protocol . the cells were centrifuged on a slide glass at 400 rpm for 5 minutes to fix them onto the slide glass . cell staining was performed in the same manner as described above . consequently , hematocytes that were concentrated using anti - cd34 antibodies ( fig1 d ) showed a higher expression of ys68 compared to hematocytes that passed through the cd34 column ( fig1 c ). therefore , ys68 expression is anticipated in less differentiated cd34 positive hematocytes . using cdna prepared from mouse embryonic liver as a template , cdna encoding the n - terminal region ( amino acids 5 - 1148 ) and c - terminal region ( amino acids 981 - 2243 ) of mouse ys68 were amplified by pcr . the amplified cdnas were inserted downstream of the flag region of animal cell expression vector pefbose - f to produce pefbose - f - ys68 ( 5 - 1148 ) and pefbose - f - ys68 ( 981 - 2243 ) that expresses the n - terminal region of ys68 and the c - terminal region of ys68 , respectively . the expression vectors were then transfected into cos - 7 cells using lipofectamine 2000 ( gibco ), and 24 hours later , the cells were immobilized with methanol . to investigate the localizations of each ys68 expressed within the cells , the cells were reacted with anti - flag antibody , followed by peroxidase - labeled anti - mouse igg , and finally substrate was added for visualization . due to the multiple nuclear transport signals in the ys68 c - terminal region ( fig9 ), localization of ys68 in the nucleus was anticipated ; however , endogenous ys68 was localized not only in the nucleus but also around the nucleus ( fig1 ). additionally , constructs lacking the ys68 n - terminal region or the c - terminal region were prepared and were expressed in cos cells , and their localizations were investigated . the results confirmed that ys68 lacking the c - terminal region had strong tendency to localize in the cytoplasm , and ys68 lacking the n - terminal region in the nucleus ( fig1 ). these results suggested the possibility that the n - terminal region is inhibiting the transfer of ys68 into the nucleus . since two wd repeats necessary for protein interaction exist in the n - terminal region , it was speculated that binding of this region to some molecule might possibly inhibit the transfer into the nucleus . the present invention provides novel “ ys68 ” proteins predicted to be involved in primitive hematopoiesis and genes encoding the proteins . the genes may be utilized as markers for hematopoietic cells involved in primitive hematopoiesis and as factors regulating hematopoiesis . in addition , they may be utilized for purification and cloning of new factors involved in hematopoiesis , and even as tools for drug development for various diseases arising due to abnormalities in expression of the genes of this invention caused by abnormalities in expression regulation in vivo . further , the “ ys68 ” genes of this invention may be involved in blood tumors . therefore , drug development against tumors utilizing the proteins of this invention is anticipated . by designing medicaments that target the genes of this invention , development of drugs that have new mechanisms of action may be enabled . proteins and genes derived from humans are especially preferred in drug development compared to those derived from other organisms t cag att ctg aag aat aat ctc atg agt gat cgt gac cct cga ttg cgg 49 gaa aga tcg gtg act cga aat tct ata tta gac cag tat ggg aaa atc 97 glu arg ser val thr arg asn ser ile leu asp gln tyr gly lys ile cta cct aga gtc cag aga aag tta gct gtt gag cga gct aag cct tac 145 cac ctg tcg aca tcc tca gtt ttt cat gaa gtt tct aga ccc aaa ccg 193 tta tcg gca ttt cca aag aaa gct ata act gga aca gtg tta acc cga 241 leu ser ala phe pro lys lys ala ile thr gly thr val leu thr arg tct acg ttc atc agc aat gtt tta tct aaa att gga gag gtg tgg gca 289 ser thr phe ile ser asn val leu ser lys ile gly glu val trp ala agt cat gag cct aga aat ggc gtc tca ctt ttt aac agt cct aaa aca 337 ser his glu pro arg asn gly val ser leu phe asn ser pro lys thr gaa cag cca tct cct gta gta cac tct ttc cca cac cca gag ctt cct 385 gag gcg ttt gtt gga act cca att tca aat aca tcc cag aga att tct 433 glu ala phe val gly thr pro ile ser asn thr ser gln arg ile ser aga tta ctg gat ttg gtt gtc cat cct gta ccc cag cct tct cag tgt 481 ttg gag ttt att caa caa agt ccc aca aga tct cct ttg tgt ctg ctg 529 tcc agt tcg tta cca tta agt tca cag ttt aaa agg cca cat cag aat 577 acc tcc agg cct tca gag ttg ctt tta ctt gag act cct ctc ata gtt 625 aag aaa gct aaa tct ttg gct ctg tca gcc acg tct tct gga ttt gcc 673 gag ttt act cct cca tcc atc ctt agg tct ggt ttt cga aca aca cct 721 tta gca tct ccc tct ttg tca cct gga aga tct ctc act ccg cct ttc 769 aga gtt aaa gaa aca agg att tca ttc atg gaa gaa ggc atg aat aca 817 arg val lys glu thr arg ile ser phe met glu glu gly met asn thr cac tgg act gat aga gct aca gat gac cga aat aca aaa gcg ttt gtt 865 agc aca tct ttc cat aaa tgt gga ctt cca gca gaa act gag tgg atg 913 ser thr ser phe his lys cys gly leu pro ala glu thr glu trp met aag acc agt gat aag aat aca tat ttt cct ctg gat gtc cct gca aag 961 lys thr ser asp lys asn thr tyr phe pro leu asp val pro ala lys ggc cct cag aaa gtg gtg gca gag tca ctg gct acc cat tca gga agg 1009 gly pro gln lys val val ala glu ser leu ala thr his ser gly arg ctg gag aaa ctg gat gtg agc aaa gaa gac agc aca gct tcc acc agg 1057 tca gac cag acc tcc tta gag tat cat gac gca cca tca cca gaa gac 1105 ttg gaa ggt gct gtt ttt gtg tct ccc aag cca gca tct tcc tcc act 1153 gaa cta act act aat tca act cta caa aca gag agg gat aat gat aaa 1201 gat gcg ttt aag tca gaa ggt act cct tca ccc gtg aag aaa caa ata 1249 asp ala phe lys ser glu gly thr pro ser pro val lys lys gln ile ggc acg gga gac gct gca gtg gaa gca ttt tca gaa ctg agt cgc tta 1297 gac cct gtt gaa aga gct gaa gct tct ttt ggt gtg tcg tca gtc tgt 1345 gaa ggg gaa acc tcc act tca aac tcc aag acg tca gtt ctg gat gga 1393 atc gtg cct att gag agc cga acc tcc ata ctt aca gca gac cac aaa 1441 ile val pro ile glu ser arg thr ser ile leu thr ala asp his lys gag tct gtg gcc aac acg gtt gca gat gtt gaa agc tct ggg tcc acc 1489 agc tcc aag tgc ccg gtt acc tct gaa cgc agc ctc ggc caa aaa cta 1537 ser ser lys cys pro val thr ser glu arg ser leu gly gln lys leu aca tta aac tta aaa gaa gat gaa ata gaa gct cat gta cca aag gag 1585 thr leu asn leu lys glu asp glu ile glu ala his val pro lys glu aac gtt ggt tta cca gaa gaa agc cct cga att tct gct gct cct tct 1633 gat act cac gag att cat cta att gga tgt gaa aat ctt gaa gtt caa 1681 asp thr his glu ile his leu ile gly cys glu asn leu glu val gln aat tca gaa gag gag gcc aag aat ctt tca ttt gat gag ttg tat ccc 1729 tta ggg gca gag aaa ctt gag tat aat ctc agt act att gag cag cag 1777 leu gly ala glu lys leu glu tyr asn leu ser thr ile glu gln gln ttt tgt gac ttg cct gat gac aaa gac tct gct gaa tgt gat gct gct 1825 gaa gta gac ggg gaa ctt ttt gtg gcc cag agc aac ttt acc ctg att 1873 glu val asp gly glu leu phe val ala gln ser asn phe thr leu ile tta gaa ggt gaa gaa gga gaa gct gag gca agc gac tct gca gca cct 1921 aat atg tta ccg aaa tcg acc aag gaa aaa cct gtg tgc tac agg gaa 1969 asn met leu pro lys ser thr lys glu lys pro val cys tyr arg glu ccc cat aat cag gag cgc gtt aca gat ttg cca tct gct gtg act gct 2017 pro his asn gln glu arg val thr asp leu pro ser ala val thr ala gac caa gaa tcc cac aag gta gag act tta ccg tat gtg cct gaa ccg 2065 asp gln glu ser his lys val glu thr leu pro tyr val pro glu pro gtt aaa gtg gca att gca gaa aat ctg ttg gat gta att aaa gac acc 2113 aga agt aag gaa gca act ccc gtg gca gca ggt gag gct ggt gat gag 2161 gac gga gca gtg ata gtc tca aag gct gca cat tcg tcc agg ctg aca 2209 asp gly ala val ile val ser lys ala ala his ser ser arg leu thr aac tct aca ccg aag act gtt aag gaa cca cgt gca gag act gta aat 2257 acc agc cag agt gat gac atg gtt tct tct aga act ctc aca aga agg 2305 cag cat gcc cta agc ctg aat gtc aca tca gaa caa gag cct tca gca 2353 gtt gcc act cct aag aag aga act aga aaa att aaa gaa act cct gag 2401 tct tct gaa agg acc tgt tct gac cta aaa gta gca cct gag aac caa 2449 ser ser glu arg thr cys ser asp leu lys val ala pro glu asn gln ctg aca gct cag aat cct ccc gct cct agg aga aga aag aag aag gac 2497 gtt agc caa ggc aca ctg cca agt tct ggt gct gtg gag ccg gag ccg 2545 gaa cct cag ggt acg ccg gga aga ctg agg ctg aga acg cag cca ccc 2593 gag cca gca gct gaa gaa act cct tct aga aca aaa gtc agg ctt tca 2641 tct gtt aga aag gga acc cct aga aga ctt aag aag tct gta gaa aat 2689 ggg caa agt ata gaa att cta gat gat ctc aaa ggg agt gag gca gca 2737 agt cat gac ggg act gtc aca gag ctg agg aat gcc aat tta gaa gat 2785 ser his asp gly thr val thr glu leu arg asn ala asn leu glu asp act cag aat atg gag tat aaa caa gat gaa cac agt gac cag caa ccg 2833 thr gln asn met glu tyr lys gln asp glu his ser asp gln gln pro cct cta aaa cga aag agg gtc aga gag aga gaa gtt agt gtg tca agt 2881 gtg aca gaa gag cca aag ctt gac tca tcc cag ttg cct ctt cag aca 2929 gga ctc gat gta cct gcc acc cct agg aaa cgt ggt aga ccc agg aag 2977 gta gtt ccc tta gaa gct gac ggt ggc aca act ggt aag gaa cag aca 3025 agt cct cag aag aaa gat gtt ccg gtt gtc cgg aga tct aca cgg aac 3073 acc cca gct aga aat gtg agt act tta naa aaa tca gtt tta gtg cca 3121 aat aag gaa gct gct cta gtg gtg aca tct aag agg aga cct aca aag 3169 aag tct gca gag gaa agc tca aaa gat cca tca gcg gca gtc tca gac 3217 tgg gcg ggt gga gca gcc cac aca gag tcc gct gac cga agg gac gga 3265 ctg ctt gcc gcc gct gct ctc acg cca tct gcc cag ggc aca agg act 3313 agg tct aga agg acc atg ttg ttg acg gac att tct gaa ccc aaa act 3361 gag cct tta ttt cct cct cct tca gtg aag gtt cca aag aaa aaa tca 3409 aaa gct gag aac atg gag gcc gca gcc cag ctg aaa gaa ttg gtg tca 3457 gat tta tct tct cag ttt gtt gtt tcc cct cct gcc ttg aga acc agg 3505 cag aaa agt ata tcc aat act tcc aag ctt cta ggt gaa ctg gag agt 3553 gac cct aaa cca tta gag atc ata gaa caa aaa cca aaa aga agc agg 3601 act gtg aag aca aga gca agc aga aac aca gga aaa gga agt tct tgg 3649 tca cct cct cct gta gaa att aag ctg gtt tct ccc ttg gcg agt cca 3697 gtg gat gaa ata aag acc ggc aag cca aga aaa act gca gaa ata gca 3745 gga aaa act ctt gga agg ggc aga aag aag cca tct tct ttt cca aag 3793 caa att tta cgc agg aaa atg ctg taatttttag cccaagattt taacacgcac 3847 glu arg ser val thr arg asn ser ile leu asp gln tyr gly lys ile leu ser ala phe pro lys lys ala ile thr gly thr val leu thr arg ser thr phe ile ser asn val leu ser lys ile gly glu val trp ala ser his glu pro arg asn gly val ser leu phe asn ser pro lys thr glu ala phe val gly thr pro ile ser asn thr ser gln arg ile ser arg val lys glu thr arg ile ser phe met glu glu gly met asn thr ser thr ser phe his lys cys gly leu pro ala glu thr glu trp met lys thr ser asp lys asn thr tyr phe pro leu asp val pro ala lys gly pro gln lys val val ala glu ser leu ala thr his ser gly arg asp ala phe lys ser glu gly thr pro ser pro val lys lys gln ile ile val pro ile glu ser arg thr ser ile leu thr ala asp his lys ser ser lys cys pro val thr ser glu arg ser leu gly gln lys leu thr leu asn leu lys glu asp glu ile glu ala his val pro lys glu asp thr his glu ile his leu ile gly cys glu asn leu glu val gln leu gly ala glu lys leu glu tyr asn leu ser thr ile glu gln gln glu val asp gly glu leu phe val ala gln ser asn phe thr leu ile asn met leu pro lys ser thr lys glu lys pro val cys tyr arg glu pro his asn gln glu arg val thr asp leu pro ser ala val thr ala asp gln glu ser his lys val glu thr leu pro tyr val pro glu pro asp gly ala val ile val ser lys ala ala his ser ser arg leu thr ser ser glu arg thr cys ser asp leu lys val ala pro glu asn gln ser his asp gly thr val thr glu leu arg asn ala asn leu glu asp thr gln asn met glu tyr lys gln asp glu his ser asp gln gln pro gag aag ttg tgg aaa cga gat gaa gga ggc aca gga aaa tat cct cct 48 gct agt ctg cat gca gta ctt gat atg tac cta tta gac ggc gtt act 96 gaa gca gcc aaa cac tct att acc att tat ttg cta ctt gat att atg 144 glu ala ala lys his ser ile thr ile tyr leu leu leu asp ile met tat tcc ttt ccc aac aaa aca gac act ccc att gaa tct ttc cca act 192 gta ttt gcc att tct tgg ggc caa gtt aaa ctt att cag ggg ttt tgg 240 ttg ata gat cat aat gac tat gag agt ggt ttg gat ctt ttg ttt cat 288 cca gct act gca aaa cct ttg tca tgg caa cat tca aag att att cag 336 gca ttc atg agt cag ggc gag cac aga caa gcc ctc aga tat att cag 384 ala phe met ser gln gly glu his arg gln ala leu arg tyr ile gln aca atg aag cca aca gtg tcc agt ggt aac gat gtt atc ctt cac ctc 432 thr met lys pro thr val ser ser gly asn asp val ile leu his leu act gtt ttg ctt ttt aat agg tgt atg gtt gaa gcc tgg aat ttt ttg 480 thr val leu leu phe asn arg cys met val glu ala trp asn phe leu cgg caa cat tgc aat agg ttg aat ata gag gag tta ctg aag cac atg 528 tat gaa gtc tgt cag gaa atg ggc ttg atg gaa gat tta ctg aag tta 576 cca ttt aca gac act gag cag gaa tgt tta gtg aaa ttt ttg cag tcc 624 pro phe thr asp thr glu gln glu cys leu val lys phe leu gln ser agt gcc agc gtt cag aat cat gaa ttc ctt tta gtg cac cat ttg cag 672 cgt gcc aat tat gtg cct gcc ttg aag ctg aac caa act ctg aag att 720 arg ala asn tyr val pro ala leu lys leu asn gln thr leu lys ile aat gtt atg aat gat cgt gat cct cgt ttg cgg gag aga tca ctg gct 768 cga aat tct ata tta gac cag tat gga aaa atc ctt cct aga gtc cat 816 arg asn ser ile leu asp gln tyr gly lys ile leu pro arg val his cga aaa tta gcc att gaa cga gct aag cct tat cat ctg tca aca tca 864 arg lys leu ala ile glu arg ala lys pro tyr his leu ser thr ser tca gtt ttt cga tta gtt tct aga ccc aaa cca tta tca gca gtt cca 912 aag caa gtt gta aca gga act gtg ttg aca aga tct gtt ttc atc aac 960 lys gln val val thr gly thr val leu thr arg ser val phe ile asn aat gtg tta tct aaa att gga gaa gtt tgg gca agc aaa gaa cct ata 1008 asn val leu ser lys ile gly glu val trp ala ser lys glu pro ile aat agc acc aca cct ttc aat agt tct aaa ata gaa gaa cca tct cct 1056 ata gtg tat tcg ctc cca gct cca gag ctg cct gag gca ttt ttt gga 1104 aca cca att tca aaa gca tca caa aaa att tct aga ctg cta gat ttg 1152 gtt gtt cag cct gtc ccc cgg cct tct cag tgt tcg gag ttt att cag 1200 caa agc tcc atg aaa tct cct ttg tac cta gta tcc cgt tca ctg ccc 1248 tca agt tcg caa tta aaa gga tcg cct cag gcc atc tcc agg gct tca 1296 gaa tta cat ttg ctt gaa act cct ctt gta gtt aag aaa gct aaa agt 1344 ttg gcc atg tca gtt act act tct gga ttt tct gag ttc act cct cag 1392 leu ala met ser val thr thr ser gly phe ser glu phe thr pro gln tcc atc ctg agg tct act cct cga tca aca cct tta gca tct ccc tct 1440 cca tca cct gga agg tct cct caa cga ctt aaa gaa act aga att tca 1488 ttt gtg gaa gaa gat gtc cac cca aaa tgg att cct ggg gct gca gat 1536 phe val glu glu asp val his pro lys trp ile pro gly ala ala asp gat agc aaa tta gaa gta ttt act aca cct aaa aaa tgt gca gtt cca 1584 asp ser lys leu glu val phe thr thr pro lys lys cys ala val pro gtg gaa act gaa tgg ccg aag agc aaa gat agg acc aca tct ttt ttc 1632 ctg aac agc cct gaa aag gag cat caa gaa atg gat gag ggg tca caa 1680 leu asn ser pro glu lys glu his gln glu met asp glu gly ser gln agt tta gag aaa ctg gat gtg agc aaa gga aac agc agt gtt tca atc 1728 aca tcc gat gag act acc tta gag tat cag gat gca ccg tca ccg gaa 1776 gac ctt gaa gag act gtt ttc acg gcc tct aag ccc aaa agc tct tcc 1824 act gca cta act act aat gta act gaa caa act gaa aag gat gga gat 1872 aaa gat gta ttt gca tca gaa gta act cct tca gac cta cag aaa caa 1920 lys asp val phe ala ser glu val thr pro ser asp leu gln lys gln atg ggc aat tta gaa gat gca gaa aca aag gat ctc tta gtt gca gca 1968 gag gca ttt tca gaa ttg aat cac tta agc ccg gtt caa gga act gaa 2016 glu ala phe ser glu leu asn his leu ser pro val gln gly thr glu gct tct ctt tgt gca cca tca gtc tat gaa ggg aaa atc ttc acc cag 2064 ala ser leu cys ala pro ser val tyr glu gly lys ile phe thr gln aag tcc aag gta cca gtg ttg gac gaa gga tta aca tct gtt gaa acc 2112 tac acc cct gca att aga gca aat gac aat aaa tct atg gct gat gtc 2160 tyr thr pro ala ile arg ala asn asp asn lys ser met ala asp val ctt ggt gat ggt gga aac tcc tcg ctc act atc tct gaa ggt cct att 2208 gtc tct gag cgc agg ctt aac cag gaa gta gcg ctg aac tta aaa gaa 2256 gat cat gaa gta gaa gtt ggt gta cta aaa gaa agt gtt gac tta cca 2304 gaa gaa aag ctt cca att tct gac agc cct cct gat act caa gaa att 2352 cat gtg att gaa caa gaa aag ctt gaa gct caa gat tca gga gaa gag 2400 his val ile glu gln glu lys leu glu ala gln asp ser gly glu glu gct agg aat ctt tca ttt aat gag tta tat ccc tct gga aca ctt aag 2448 ala arg asn leu ser phe asn glu leu tyr pro ser gly thr leu lys ctt cag tac aat ttt gat act att gac caa cag ttt tgt gac tta gct 2496 gat aac aaa gac act gct gaa tgt gac att gct gaa gta gat ggg gaa 2544 ctt ttt gtg gct caa agc aac ttt acc ttg ata ttg gaa ggt gaa gaa 2592 leu phe val ala gln ser asn phe thr leu ile leu glu gly glu glu gga gaa gtt gag cca ggt gat ttt gca tca tct gat gtg tta cct aaa 2640 gca gct aac aca gca act gaa gaa aaa ctt gta tgc agt ggg gaa aat 2688 gat aat cat gga caa att gca aat ttg cca tct gcc gta act agt gac 2736 asp asn his gly gln ile ala asn leu pro ser ala val thr ser asp caa aag tcc caa aaa gta gac act tta cca tat gtg cct gaa cct att 2784 gln lys ser gln lys val asp thr leu pro tyr val pro glu pro ile aaa gta gca att gca gaa aat tta cta gat gta att aaa gac aca aga 2832 agt aaa gaa att act tca gat aca atg gaa cag tcc att cat gaa aca 2880 ata cct tta gtg agc caa aac ata atg tgt ccc act aaa ttg gtc aaa 2928 ile pro leu val ser gln asn ile met cys pro thr lys leu val lys tct gca ttt aag act gct cag gaa aca agc aca atg act atg aat gtc 2976 agc cag gtt gat gac gtg gtt tcc tcc aaa act cgt acg aga ggt caa 3024 cgt atc caa aac gtg aat gtc aaa tca gca caa cag gaa gca tca gca 3072 gat gtt gct act cct aag atg cca ggg cag tca gtc agg aag aaa act 3120 asp val ala thr pro lys met pro gly gln ser val arg lys lys thr agg aag gca aaa gaa att tct gaa gct tct gaa aac atc tat tct gat 3168 gtc aga gga cta ttt cag aac cag caa ata cct caa aat tct gtt acg 3216 val arg gly leu phe gln asn gln gln ile pro gln asn ser val thr cct agg aga gga agg aga aag aaa gaa gtt aat cag gac ata cta gaa 3264 pro arg arg gly arg arg lys lys glu val asn gln asp ile leu glu aac acc agt tct gtg gaa caa gaa tta cag atc act aca ggt agg gaa 3312 tca aaa aga tta aaa tca tct cag ctg ttg gaa cca gca gtt gaa gaa 3360 act act aaa aaa gaa gtt aag gtt tca tct gtt aca aaa agg act cct 3408 aga aga att aaa aga tct gta gaa aat cag gaa agt gtt gaa att ata 3456 aat gat cta aaa gtt agt acg gta aca agt cct agc aga atg atc aga 3504 asn asp leu lys val ser thr val thr ser pro ser arg met ile arg aaa ttg aga agt act aat tta gat gct tct gaa aat aca gga aat aag 3552 caa gat gat aaa tcc agt gac aag cag ctg cgt att aaa cat gtt aga 3600 agg gtc aga ggg aga gaa gtt agt cca tca gat gtg aga gaa gac tcc 3648 aac ctt gag tca tct cag ttg act gtt caa gca gaa ttt gat atg tct 3696 asn leu glu ser ser gln leu thr val gln ala glu phe asp met ser gcc ata cct aga aaa cgt ggt aga cca aga aaa atc aat cca tct gaa 3744 gat gta gga tct aag gct gtt aag gaa gag aga agc ccc aag aag aaa 3792 gaa gct ccc agc att aga agg aga tct aca aga aat acc cca gct aaa 3840 agt gaa aat gtt gat gtt gga aaa cca gct tta gga aaa tcc att tta 3888 ser glu asn val asp val gly lys pro ala leu gly lys ser ile leu gtg cca aac gag gaa ctt tcg atg gtg atg agc tct aag aaa aaa ctt 3936 aca aaa aag act gaa agt caa agc caa aaa cgt tca ttg cac tca gta 3984 tca gaa gaa cgc aca gat gaa atg aca cat aaa gaa aca aat gag cag 4032 gaa gaa aga ttg ctc gcc aca gct tcc ttc act aaa tca tcc cgc agc 4080 agc agg act cgg tct agc aag gcc atc ttg ttg ccg gac ctt tct gaa 4128 cca aac aat gag cct tta ttt tct cca gcg tca gaa gtt cca agg aaa 4176 gca aaa gct aaa aaa ata gag gtt cct gca cag ctg aaa gaa tta gtt 4224 tcg gat tta tct tct cag ttt gtc atc tca cct cct gct tta agg agc 4272 aga caa aaa aac aca tcc aat aag aac aag ctt gaa gat gaa ctg aaa 4320 gat gat gca caa tca gta gaa act ctg gga aag cca aaa gcg aaa cga 4368 asp asp ala gln ser val glu thr leu gly lys pro lys ala lys arg atc agg acg tca aaa aca aaa caa gca agc aaa aac aca gaa aaa gaa 4416 agt gct tgg tca ctt cct ccc ata gaa att cgg ctg att tcc ccc ttg 4464 gct agc cca gct gac gga gtc aag agc aaa cca aga aaa act aca gaa 4512 gtg aca gga aca ggt ctt gga agg aac aga aag aaa ctg tct tcc tat 4560 cca aag caa att tta cgc aga aaa atg ctg taatttcttg ggaagatttt 4610 glu ala ala lys his ser ile thr ile tyr leu leu leu asp ile met ala phe met ser gln gly glu his arg gln ala leu arg tyr ile gln thr met lys pro thr val ser ser gly asn asp val ile leu his leu thr val leu leu phe asn arg cys met val glu ala trp asn phe leu pro phe thr asp thr glu gln glu cys leu val lys phe leu gln ser arg ala asn tyr val pro ala leu lys leu asn gln thr leu lys ile arg asn ser ile leu asp gln tyr gly lys ile leu pro arg val his arg lys leu ala ile glu arg ala lys pro tyr his leu ser thr ser lys gln val val thr gly thr val leu thr arg ser val phe ile asn asn val leu ser lys ile gly glu val trp ala ser lys glu pro ile leu ala met ser val thr thr ser gly phe ser glu phe thr pro gln phe val glu glu asp val his pro lys trp ile pro gly ala ala asp asp ser lys leu glu val phe thr thr pro lys lys cys ala val pro leu asn ser pro glu lys glu his gln glu met asp glu gly ser gln lys asp val phe ala ser glu val thr pro ser asp leu gln lys gln glu ala phe ser glu leu asn his leu ser pro val gln gly thr glu ala ser leu cys ala pro ser val tyr glu gly lys ile phe thr gln tyr thr pro ala ile arg ala asn asp asn lys ser met ala asp val his val ile glu gln glu lys leu glu ala gln asp ser gly glu glu ala arg asn leu ser phe asn glu leu tyr pro ser gly thr leu lys leu phe val ala gln ser asn phe thr leu ile leu glu gly glu glu asp asn his gly gln ile ala asn leu pro ser ala val thr ser asp gln lys ser gln lys val asp thr leu pro tyr val pro glu pro ile ile pro leu val ser gln asn ile met cys pro thr lys leu val lys asp val ala thr pro lys met pro gly gln ser val arg lys lys thr val arg gly leu phe gln asn gln gln ile pro gln asn ser val thr pro arg arg gly arg arg lys lys glu val asn gln asp ile leu glu asn asp leu lys val ser thr val thr ser pro ser arg met ile arg asn leu glu ser ser gln leu thr val gln ala glu phe asp met ser ser glu asn val asp val gly lys pro ala leu gly lys ser ile leu asp asp ala gln ser val glu thr leu gly lys pro lys ala lys arg t cag att ctg aag aat aat ctc atg agt gat cgt gac cct cga ttg cgg 49 gaa aga tcg gtg act cga aat tct ata tta gac cag tat ggg aaa atc 97 glu arg ser val thr arg asn ser ile leu asp gln tyr gly lys ile cta cct aga gtc cag aga aag tta gct gtt gag cga gct aag cct tac 145 cac ctg tcg aca tcc tca gtt ttt cat gaa gtt tct aga ccc aaa ccg 193 tta tcg gca ttt cca aag aaa gct ata act gga aca gtg tta acc cga 241 leu ser ala phe pro lys lys ala ile thr gly thr val leu thr arg tct acg ttc atc agc aat gtt tta tct aaa att gga gag gtg tgg gca 289 ser thr phe ile ser asn val leu ser lys ile gly glu val trp ala agt cat gag cct aga aat ggc gtc tca ctt ttt aac agt cct aaa aca 337 ser his glu pro arg asn gly val ser leu phe asn ser pro lys thr gaa cag cca tct cct gta gta cac tct ttc cca cac cca gag ctt cct 385 gag gcg ttt gtt gga act cca att tca aat aca tcc cag aga att tct 433 glu ala phe val gly thr pro ile ser asn thr ser gln arg ile ser aga tta ctg gat ttg gtt gtc cat cct gta ccc cag cct tct cag tgt 481 ttg gag ttt att caa caa agt ccc aca aga tct cct ttg tgt ctg ctg 529 tcc agt tcg tta cca tta agt tca cag ttt aaa agg cca cat cag aat 577 acc tcc agg cct tca gag ttg ctt tta ctt gag act cct ctc ata gtt 625 aag aaa gct aaa tct ttg gct ctg tca gcc acg tct tct gga ttt gcc 673 gag ttt act cct cca tcc atc ctt agg tct ggt ttt cga aca aca cct 721 tta gca tct ccc tct ttg tca cct gga aga tct ctc act ccg cct ttc 769 aga gtt aaa gaa aca agg att tca ttc atg gaa gaa ggc atg aat aca 817 arg val lys glu thr arg ile ser phe met glu glu gly met asn thr cac tgg act gat aga gct aca gat gac cga aat aca aaa gcg ttt gtt 865 agc aca tct ttc cat aaa tgt gga ctt cca gca gaa act gag tgg atg 913 ser thr ser phe his lys cys gly leu pro ala glu thr glu trp met aag acc agt gat aag aat aca tat ttt cct ctg gat gtc cct gca aag 961 lys thr ser asp lys asn thr tyr phe pro leu asp val pro ala lys ggc cct cag aaa gtg gtg gca gag tca ctg gct acc cat tca gga agg 1009 gly pro gln lys val val ala glu ser leu ala thr his ser gly arg ctg gag aaa ctg gat gtg agc aaa gaa gac agc aca gct tcc acc agg 1057 tca gac cag acc tcc tta gag tat cat gac gca cca tca cca gaa gac 1105 ttg gaa ggt gct gtt ttt gtg tct ccc aag cca gca tct tcc tcc act 1153 gaa cta act act aat tca act cta caa aca gag agg gat aat gat aaa 1201 gat gcg ttt aag tca gaa ggt act cct tca ccc gtg aag aaa caa ata 1249 asp ala phe lys ser glu gly thr pro ser pro val lys lys gln ile ggc acg gga gac gct gca gtg gaa gca ttt tca gaa ctg agt cgc tta 1297 gac cct gtt gaa aga gct gaa gct tct ttt ggt gtg tcg tca gtc tgt 1345 gaa ggg gaa acc tcc act tca aac tcc aag acg tca gtt ctg gat gga 1393 atc gtg cct att gag agc cga acc tcc ata ctt aca gca gac cac aaa 1441 ile val pro ile glu ser arg thr ser ile leu thr ala asp his lys gag tct gtg gcc aac acg gtt gca gat gtt gaa agc tct ggg tcc acc 1489 agc tcc aag tgc ccg gtt acc tct gaa cgc agc ctc ggc caa aaa cta 1537 ser ser lys cys pro val thr ser glu arg ser leu gly gln lys leu aca tta aac tta aaa gaa gat gaa ata gaa gct cat gta cca aag gag 1585 thr leu asn leu lys glu asp glu ile glu ala his val pro lys glu aac gtt ggt tta cca gaa gaa agc cct cga att tct gct gct cct tct 1633 gat act cac gag att cat cta att gga tgt gaa aat ctt gaa gtt caa 1681 asp thr his glu ile his leu ile gly cys glu asn leu glu val gln aat tca gaa gag gag gcc aag aat ctt tca ttt gat gag ttg tat ccc 1729 tta ggg gca gag aaa ctt gag tat aat ctc agt act att gag cag cag 1777 leu gly ala glu lys leu glu tyr asn leu ser thr ile glu gln gln ttt tgt gac ttg cct gat gac aaa gac tct gct gaa tgt gat gct gct 1825 gaa gta gac ggg gaa ctt ttt gtg gcc cag agc aac ttt acc ctg att 1873 glu val asp gly glu leu phe val ala gln ser asn phe thr leu ile tta gaa ggt gaa gaa gga gaa gct gag gca agc gac tct gca gca cct 1921 aat atg tta ccg aaa tcg acc aag gaa aaa cct gtg tgc tac agg gaa 1969 asn met leu pro lys ser thr lys glu lys pro val cys tyr arg glu ccc cat aat cag gag cgc gtt aca gat ttg cca tct gct gtg act gct 2017 pro his 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tct aag agg aga cct aca aag 3169 aag tct gca gag gaa agc tca aaa gat cca tca gcg gca gtc tca gac 3217 tgg gcg ggt gga gca gcc cac aca gag tcc gct gac cga agg gac gga 3265 ctg ctt gcc gcc gct gct ctc acg cca tct gcc cag ggc aca agg act 3313 agg tct aga agg acc atg ttg ttg acg gac att tct gaa ccc aaa act 3361 gag cct tta ttt cct cct cct tca gtg aag gtt cca aag aaa aaa tca 3409 aaa gct gag aac atg gag gcc gca gcc cag ctg aaa gaa ttg gtg tca 3457 gat tta tct tct cag ttt gtt gtt tcc cct cct gcc ttg aga acc agg 3505 cag aaa agt ata tcc aat act tcc aag ctt cta ggt gaa ctg gag agt 3553 gac cct aaa cca tta gag atc ata gaa caa aaa cca aaa aga agc agg 3601 act gtg aag aca aga gca agc aga aac aca gga aaa gga agt tct tgg 3649 tca cct cct cct gta gaa att aag ctg gtt tct ccc ttg gcg agt cca 3697 gtg gat gaa ata aag acc ggc aag cca aga aaa act gca gaa ata gca 3745 gga aaa act ctt gga agg ggc aga aag aag cca tct tct ttt cca aag 3793 caa att tta cgc agg aaa atg ctg taatttttag cccaagattt taacacgcac 3847 glu arg ser val thr arg asn ser ile leu asp gln tyr gly lys ile leu ser ala phe pro lys lys ala ile thr gly thr val leu thr arg ser thr phe ile ser asn val leu ser lys ile gly glu val trp ala ser his glu pro arg asn gly val ser leu phe asn ser pro lys thr glu ala phe val gly thr pro ile ser asn thr ser gln arg ile ser arg val lys glu thr arg ile ser phe met glu glu gly met asn thr ser thr ser phe his lys cys gly leu pro ala glu thr glu trp met lys thr ser asp lys asn thr tyr phe pro leu asp val pro ala lys gly pro gln lys val val ala glu ser leu ala thr his ser gly arg asp ala phe lys ser glu gly thr pro ser pro val lys lys gln ile ile val pro ile glu ser arg thr ser ile leu thr ala asp his lys ser ser lys cys pro val thr ser glu arg ser leu gly gln lys leu thr leu asn leu lys glu asp glu ile glu ala his val pro lys glu asp thr his glu ile his leu ile gly cys glu asn leu glu val gln leu gly ala glu lys leu glu tyr asn leu ser thr ile glu gln gln glu val asp gly glu leu phe val ala gln ser asn phe thr leu ile asn met leu pro lys ser thr lys glu lys pro val cys tyr arg glu pro his asn gln glu arg val thr asp leu pro ser ala val thr ala asp gln glu ser his lys val glu thr leu pro tyr val pro glu pro asp gly ala val ile val ser lys ala ala his ser ser arg leu thr ser ser glu arg thr cys ser asp leu lys val ala pro glu asn gln ser his asp gly thr val thr glu leu arg asn ala asn leu glu asp thr gln asn met glu tyr lys gln asp glu his ser asp gln gln pro tggcagt atg caa gac ttg aca gct caa gtg act agt gat ctc ctg cat 49 ttc cca gaa gtg act att gaa gct ctt gga gaa gat gag ata aca tta 97 gag tcc gtg ctt cgt gga aag ttt gct gca ggg aaa aat gga cta gca 145 tgc tta gct tgt ggt cca caa ctt gaa gtt gta aac tcc tta aca gga 193 cys leu ala cys gly pro gln leu glu val val asn ser leu thr gly gag cgg tta tct gca tat aga ttc agt gga gta aat gaa cag cct cct 241 glu arg leu ser ala tyr arg phe ser gly val asn glu gln pro pro gta gtc ctt gca gtg aaa gaa ttc tct tgg cat aag agg act gga ttg 289 val val leu ala val lys glu phe ser trp his lys arg thr gly leu tta ata gga ttg gaa gaa gca gat ggg agt gtt ctt tgt ctt tat gac 337 ctt ggt ata tca aga gtg gtc aaa gca gtt gtt ctt cct gga agg gta 385 aca gct atc gag cct ata att aac cat gga gga gcc agt gcg agt acc 433 cag cat tta cat cca agt ctc cgg tgg ctt ttt ggc gtg gcc gct gtg 481 gln his leu his pro ser leu arg trp leu phe gly val ala ala val gtg act gat gtt gga cag atc ctt ctt att gac ctg tgt ttg gat gac 529 ttg tcc tgc agt cag aat gaa gtt gag gca tca gac ctt gaa gtt atc 577 act ggt atc cca gct gaa gta cca cac atc aga gag aga gtg atg aga 625 gag ggg cgc cac ctg tgc ttc cag tta gta agc cca ttg gga gta gcc 673 glu gly arg his leu cys phe gln leu val ser pro leu gly val ala att tct act ctg agt tac atc aac agg aca aat cag ctt gct gtg ggt 721 ile ser thr leu ser tyr ile asn arg thr asn gln leu ala val gly ttt tct gat ggc tac tta gca ctt tgg aac atg aaa agc atg aaa aga 769 phe ser asp gly tyr leu ala leu trp asn met lys ser met lys arg gaa tac tat aca cag ttg gaa ggt gga agg gtt cct gtc cat gca gtt 817 glu tyr tyr thr gln leu glu gly gly arg val pro val his ala val gcc ttt caa gag cct gag aat gat cct cgt aac tgc tgt tat tta tgg 865 ala phe gln glu pro glu asn asp pro arg asn cys cys tyr leu trp gct gtt cag tcc aca caa gat agt gaa ggg gat gtt ttg agt ttg cat 913 ctg ctt cag ctg gct ttt ggt gat aga aaa tgt ttg gca tca ggg caa 961 att tta tat gag gga tta gaa tac tgc gaa gaa aga tat aca ctg gat 1009 cta gca ggt ggc acg ttc ccc tta agg gga caa act agt aat acc aaa 1057 leu ala gly gly thr phe pro leu arg gly gln thr ser asn thr lys ttg ttg gga tgc cag agt ata gag aga ttt cca tct cat gga gac aga 1105 leu leu gly cys gln ser ile glu arg phe pro ser his gly asp arg gaa gaa agt atg aga gaa gct ctg tct ccc gat acc agc gtt tct gtc 1153 ttt acc tgg caa gtg aat ata tat gga cag gga aag cct tct gtg tat 1201 phe thr trp gln val asn ile tyr gly gln gly lys pro ser val tyr tta ggg cta ttt gac ata aat cgt tgg tat cat gca caa atg ccc gat 1249 leu gly leu phe asp ile asn arg trp tyr his ala gln met pro asp tct tta aga tca gga gaa tct ctg cat aat tgc tct tat ttt gcg ttg 1297 ser leu arg ser gly glu ser leu his asn cys ser tyr phe ala leu tgg tca ttg gat tcg gtt gta agt agg act tct cca cat cac atc ttg 1345 gac ata cta gta cat gag agg agt tta aac cga ggg gtt cct cct tcc 1393 asp ile leu val his glu arg ser leu asn arg gly val pro pro ser tac cca cct cca gag caa ttt ttt aac cca agt act ttt aat ttt gat 1441 gcc act tgt ttg tta gac tct gga gtt atc cat gta act tgt gct gga 1489 ttt cag aag gag act ttg aca ttt tta aag aaa tca gga cca act ctt 1537 aat gaa gtc att cct gat agt tat aat cga tgt ctt gtt gct ggt ctc 1585 asn glu val ile pro asp ser tyr asn arg cys leu val ala gly leu ctc tca cca aga ctt att gat att cag cct tcc agt tta agt caa gaa 1633 gaa caa tta gaa gct ata ttg tca gca gca att cag aca agt tcc ttg 1681 gga ctt ttg act ggt tac atc aga aca tgg ata ata gaa gaa caa cca 1729 aat tct gct gct aat cta cga ttt gtt ctt gag tgg aca tgg aat aaa 1777 asn ser ala ala asn leu arg phe val leu glu trp thr trp asn lys gtg gtt ctc aca aaa gaa gag ttt gat agg ctt tgt gtg ccg ctg ttt 1825 gac ggt tcg tgt cgt ttt att gac cca cag act att cag tct atc cag 1873 cag tgc cat tta ctg ctt agc aac ctt agt aca gtc tta agc tgt ttt 1921 gca atg gag gcc cag ggt atc act gag aga gga ctg gtg gac ttg agc 1969 ala met glu ala gln gly ile thr glu arg gly leu val asp leu ser aac aag cac atg gtc acc cag ctt ctc tgt cag tac gca cac atg gtt 2017 asn lys his met val thr gln leu leu cys gln tyr ala his met val ctg tgg ttc tgc cac tcg ggg ctt ctg ccc gaa ggc tta gat gat gct 2065 leu trp phe cys his ser gly leu leu pro glu gly leu asp asp ala ctg cac ctg tca aga cta cgc tac aac tac cct gta att cag aac tac 2113 tat aca agt cgt cgg cag aag tct gag cgc tca ccc aga ggg aag tgg 2161 aac cac gac tgc ttg atg att gat gga tta gtc tct caa cta gga gat 2209 asn his asp cys leu met ile asp gly leu val ser gln leu gly asp gaa gtt gag aag ttg tgg aag cgg gac gaa ggt ggc aca gga aga tac 2257 cct cct gct agc atc cac gca tta ctt gat ata tat tta tta gac aac 2305 att acc gaa gca agc aaa cat gct att acc att tat ttg ctg ctt gat 2353 att atg tat tcc ttt cca aat aaa acg gat acc ccc att gaa tct ttc 2401 ile met tyr ser phe pro asn lys thr asp thr pro ile glu ser phe ccc act gcc ttt gct att tct tgg ggc caa gtt aag cta gtt caa gga 2449 pro thr ala phe ala ile ser trp gly gln val lys leu val gln gly ttt tgg cta cta gat cat aat gac tat gag aat ggt tta gac ctt ctg 2497 ttt cac cca gtt act gca aag cct gca tcg tgg caa cat tca aag ata 2545 phe his pro val thr ala lys pro ala ser trp gln his ser lys ile att gaa gct ttt atg agt cag gga gag cac aaa cag gct ctc cgg tat 2593 ile glu ala phe met ser gln gly glu his lys gln ala leu arg tyr ctt cag aca atg aag cca aca gtg tcc agt agc aat gaa gtt atc ctt 2641 leu gln thr met lys pro thr val ser ser ser asn glu val ile leu cac ctc act gtt cta ctt ttt aat aga tgc atg gtt gag gcc tgg aac 2689 his leu thr val leu leu phe asn arg cys met val glu ala trp asn tta ctg cga cag aat tca aac aga gta aat ata gag gaa tta tta aag 2737 cac gct tat gaa gtt tgt cag gag atg ggc tta atg gag gat tta ctg 2785 his ala tyr glu val cys gln glu met gly leu met glu asp leu leu aag ctg cca ttt aca aac act gag cag gaa tgc tta gtg aaa ttt tta 2833 cag tcc agt acc agt gtt gag aat cat gaa ttc ctt cta gtt cac cat 2881 tta cag cgt gcc aat tat att tct gcc ttg aaa cta aac cag att ctg 2929 aag aat aat ctc atg agt gat cgt gac cct cga ttg cgg gaa aga tcg 2977 gtg act cga aat tct ata tta gac cag tat ggg aaa atc cta cct aga 3025 val thr arg asn ser ile leu asp gln tyr gly lys ile leu pro arg gtc cag aga aag tta gct gtt gag cga gct aag cct tac cac ctg tcg 3073 val gln arg lys leu ala val glu arg ala lys pro tyr his leu ser aca tcc tca gtt ttt cat gaa gtt tct aga ccc aaa ccg tta tcg gca 3121 thr ser ser val phe his glu val ser arg pro lys pro leu ser ala ttt cca aag aaa gct ata act gga aca gtg tta acc cga tct acg ttc 3169 phe pro lys lys ala ile thr gly thr val leu thr arg ser thr phe atc agc aat gtt tta tct aaa att gga gag gtg tgg gca agt cat gag 3217 ile ser asn val leu ser lys ile gly glu val trp ala ser his glu cct aga aat ggc gtc tca ctt ttt aac agt cct aaa aca gaa cag cca 3265 pro arg asn gly val ser leu phe asn ser pro lys thr glu gln pro tct cct gta gta cac tct ttc cca cac cca gag ctt cct gag gcg ttt 3313 gtt gga act cca att tca aat aca tcc cag aga att tct aga tta ctg 3361 gat ttg gtt gtc cat cct gta ccc cag cct tct cag tgt ttg gag ttt 3409 att caa caa agt ccc aca aga tct cct ttg tgt ctg ctg tcc agt tcg 3457 tta cca tta agt tca cag ttt aaa agg cca cat cag aat acc tcc agg 3505 cct tca gag ttg ctt tta ctt gag act cct ctc ata gtt aag aaa gct 3553 aaa tct ttg gct ctg tca gcc acg tct tct gga ttt gcc gag ttt act 3601 cct cca tcc atc ctt agg tct ggt ttt cga aca aca cct tta gca tct 3649 ccc tct ttg tca cct gga aga tct ctc act ccg cct ttc aga gtt aaa 3697 gaa aca agg att tca ttc atg gaa gaa ggc atg aat aca cac tgg act 3745 glu thr arg ile ser phe met glu glu gly met asn thr his trp thr gat aga gct aca gat gac cga aat aca aaa gcg ttt gtt agc aca tct 3793 ttc cat aaa tgt gga ctt cca gca gaa act gag tgg atg aag acc agt 3841 phe his lys cys gly leu pro ala glu thr glu trp met lys thr ser gat aag aat aca tat ttt cct ctg gat gtc cct gca aag ggc cct cag 3889 asp lys asn thr tyr phe pro leu asp val pro ala lys gly pro gln aaa gtg gtg gca gag tca ctg gct acc cat tca gga agg ctg gag aaa 3937 ctg gat gtg agc aaa gaa gac agc aca gct tcc acc agg tca gac cag 3985 acc tcc tta gag tat cat gac gca cca tca cca gaa gac ttg gaa ggt 4033 gct gtt ttt gtg tct ccc aag cca gca tct tcc tcc act gaa cta act 4081 act aat tca act cta caa aca gag agg gat aat gat aaa gat gcg ttt 4129 thr asn ser thr leu gln thr glu arg asp asn asp lys asp ala phe aag tca gaa ggt act cct tca ccc gtg aag aaa caa ata ggc acg gga 4177 gac gct gca gtg gaa gca ttt tca gaa ctg agt cgc tta gac cct gtt 4225 gaa aga gct gaa gct tct ttt ggt gtg tcg tca gtc tgt gaa ggg gaa 4273 acc tcc act tca aac tcc aag acg tca gtt ctg gat gga atc gtg cct 4321 att gag agc cga acc tcc ata ctt aca gca gac cac aaa gag tct gtg 4369 ile glu ser arg thr ser ile leu thr ala asp his lys glu ser val gcc aac acg gtt gca gat gtt gaa agc tct ggg tcc acc agc tcc aag 4417 tgc ccg gtt acc tct gaa cgc agc ctc ggc caa aaa cta aca tta aac 4465 cys pro val thr ser glu arg ser leu gly gln lys leu thr leu asn tta aaa gaa gat gaa ata gaa gct cat gta cca aag gag aac gtt ggt 4513 leu lys glu asp glu ile glu ala his val pro lys glu asn val gly tta cca gaa gaa agc cct cga att tct gct gct cct tct gat act cac 4561 gag att cat cta att gga tgt gaa aat ctt gaa gtt caa aat tca gaa 4609 gag gag gcc aag aat ctt tca ttt gat gag ttg tat ccc tta ggg gca 4657 glu glu ala lys asn leu ser phe asp glu leu tyr pro leu gly ala gag aaa ctt gag tat aat ctc agt act att gag cag cag ttt tgt gac 4705 glu lys leu glu tyr asn leu ser thr ile glu gln gln phe cys asp ttg cct gat gac aaa gac tct gct gaa tgt gat gct gct gaa gta gac 4753 ggg gaa ctt ttt gtg gcc cag agc aac ttt acc ctg att tta gaa ggt 4801 gly glu leu phe val ala gln ser asn phe thr leu ile leu glu gly gaa gaa gga gaa gct gag gca agc gac tct gca gca cct aat atg tta 4849 ccg aaa tcg acc aag gaa aaa cct gtg tgc tac agg gaa ccc cat aat 4897 pro lys ser thr lys glu lys pro val cys tyr arg glu pro his asn cag gag cgc gtt aca gat ttg cca tct gct gtg act gct gac caa gaa 4945 tcc cac aag gta gag act tta ccg tat gtg cct gaa ccg gtt aaa gtg 4993 gca att gca gaa aat ctg ttg gat gta att aaa gac acc aga agt aag 5041 ala ile ala glu asn leu leu asp val ile lys asp thr arg ser lys gaa gca act ccc gtg gca gca ggt gag gct ggt gat gag gac gga gca 5089 gtg ata gtc tca aag gct gca cat tcg tcc agg ctg aca aac tct aca 5137 ccg aag act gtt aag gaa cca cgt gca gag act gta aat acc agc cag 5185 agt gat gac atg gtt tct tct aga act ctc aca aga agg cag cat gcc 5233 cta agc ctg aat gtc aca tca gaa caa gag cct tca gca gtt gcc act 5281 cct 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gct tac aga ttc agt gga gtc aat gaa cag cct cct gta 361 arg leu ser ala tyr arg phe ser gly val asn glu gln pro pro val gtt tta gct gtg aaa gaa ttc tct tgg cag aag aga act gga tta tta 409 val leu ala val lys glu phe ser trp gln lys arg thr gly leu leu ata gga ttg gaa gaa aca gaa ggg agt gtt ctc tgt ctt tat gac ctt 457 gga ata tca aaa gta gtt aaa gca gtt gtt ctt cct gga agg gta aca 505 gct att gaa cct ata att aat cat gga gga gcc agt gca agc act cag 553 cat tta cat cca agt ctg cga tgg ctt ttt gga gtg gca gct gtg gtc 601 act gat gtt gga cag atc ctt ctt att gac cta tgt ttg gat gac ttg 649 tca tgc aat caa aat gaa gtt gaa gca tca gat ctt gaa gtt cta act 697 ggt atc cca gct gaa gta cca cac att aga gaa agt gtg atg aga gaa 745 ggg cgc cat ctg tgt ttc cag tta gta agt cca aca gga aca gcc gtt 793 gly arg his leu cys phe gln leu val ser pro thr gly thr ala val tca act ctt agt tac ata agc agg aca aat cag ctt gct gca ggt ttt 841 ser thr leu ser tyr ile ser arg thr asn gln leu ala ala gly phe tct gat ggc tat cta gca ctt tgg aac atg aaa agc atg aaa aga gaa 889 ser asp gly tyr leu ala leu trp asn met lys ser met lys arg glu tat tac ata caa ttg gaa agt gga caa gtt cct gta tat gct gtc act 937 tyr tyr ile gln leu glu ser gly gln val pro val tyr ala val thr ttt caa gaa cct gag aat gat cgt cgg aat tgc tgc tac ttg tgg gct 985 phe gln glu pro glu asn asp arg arg asn cys cys tyr leu trp ala gtt cag tct aca caa gat agt gaa ggg gat gtt ttg agt ttg cat ctg 1033 ctg cag ctg gcc ttt ggt aat aga aag tgt ttg gca tca gga caa atc 1081 leu gln leu ala phe gly asn arg lys cys leu ala ser gly gln ile tta tat gag ggg tta gaa tac tgt gaa gaa aga tac acc ctg gac ctg 1129 aca ggt ggc atg ttc cct ttg agg gga cag acg agt aat acc aaa ttg 1177 thr gly gly met phe pro leu arg gly gln thr ser asn thr lys leu ttg gga tgc cag agt ata gag aaa ttt cga tct cat ggt gac agg gag 1225 leu gly cys gln ser ile glu lys phe arg ser his gly asp arg glu gaa ggc gtg aat gaa gct cta tcg cct gac act agt gtt tca gtc ttt 1273 glu gly val asn glu ala leu ser pro asp thr ser val ser val phe acc tgg cag gtg aat ata tat gga cag gga aag cct tct gta tat ttg 1321 thr trp gln val asn ile tyr gly gln gly lys pro ser val tyr leu ggg ctt ttt gat ata aat cgt tgg tat cat gca caa atg cca gat tcg 1369 gly leu phe asp ile asn arg trp tyr his ala gln met pro asp ser tta agg tca gga gaa tat cta cat aat tgc tct tat ttt gca ctg tgg 1417 leu arg ser gly glu tyr leu his asn cys ser tyr phe ala leu trp tca ttg gag tct gtt gta agt agg act tct cca cat ggc atc ttg gat 1465 ser leu glu ser val val ser arg thr ser pro his gly ile leu asp ata tta gta cat gag aga agt tta aat aga gga gtc cct cct tca tat 1513 ile leu val his glu arg ser leu asn arg gly val pro pro ser tyr cca cct ccc gag cag ttt ttt aat cca agc act tat aat ttt gat gcc 1561 act tgt ttg tta aac tcg gga gtt gtt cat tta act tgt act ggc ttt 1609 cag aag gag act ttg act ttt tta aag aaa tca ggt cca tca ctc aat 1657 gaa ctc att cct gat ggt tat aat cga tgt ctt gta gct ggc ctt ctt 1705 glu leu ile pro asp gly tyr asn arg cys leu val ala gly leu leu tcc cca aga ttt gtt gat gtt cag cct tcc agt tta agc caa gaa gaa 1753 cag tta gaa gct ata ttg tca gca gca att cag act agt tcc ctg gga 1801 ctt ttg act ggt tat atc cga aga tgg ata aca gaa gaa caa cca aat 1849 leu leu thr gly tyr ile arg arg trp ile thr glu glu gln pro asn tct gcc act aat ttg cgc ttt gtt ctt gaa tgg acg tgg aat aaa gtg 1897 ser ala thr asn leu arg phe val leu glu trp thr trp asn lys val gtt ctc aca aaa gag gaa ttt gac aga cta tgt gtg cca tta ttt gat 1945 ggt tcg tgt cat ttc atg gat cca caa act ata cag tct atc cag caa 1993 gly ser cys his phe met asp pro gln thr ile gln ser ile gln gln tgc tat ttg ctt ctt agc aat ctt aat ata gtc ttg agc tgt ttt gca 2041 tca gaa gcc cga gag atc gct gag aga gga ctg ata gac tta agc aat 2089 aag ttt gtg gtt tcc cac ctc atc tgt cag tat gca caa gtg gtt ctt 2137 lys phe val val ser his leu ile cys gln tyr ala gln val val leu tgg ttc tct cat tct ggg ctt tta cca gaa ggc ata gat gat tct gtg 2185 trp phe ser his ser gly leu leu pro glu gly ile asp asp ser val cag ttg tca agg tta tgc tac aac tac cct gta att cag aac tac tac 2233 acc agt cgt cga cag aag ttt gag cgt tta tca aga ggg aag tgg aat 2281 thr ser arg arg gln lys phe glu arg leu ser arg gly lys trp asn ccc gat tgc ttg atg att gat gga ctg gtt tct cag tta gga gag cga 2329 pro asp cys leu met ile asp gly leu val ser gln leu gly glu arg att gag aag ttg tgg aaa cga gat gaa gga ggc aca gga aaa tat cct 2377 ile glu lys leu trp lys arg asp glu gly gly thr gly lys tyr pro cct gct agt ctg cat gca gta ctt gat atg tac cta tta gac ggc gtt 2425 act gaa gca gcc aaa cac tct att acc att tat ttg cta ctt gat att 2473 atg tat tcc ttt ccc aac aaa aca gac act ccc att gaa tct ttc cca 2521 met tyr ser phe pro asn lys thr asp thr pro ile glu ser phe pro act gta ttt gcc att tct tgg ggc caa gtt aaa ctt att cag ggg ttt 2569 thr val phe ala ile ser trp gly gln val lys leu ile gln gly phe tgg ttg ata gat cat aat gac tat gag agt ggt ttg gat ctt ttg ttt 2617 trp leu ile asp his asn asp tyr glu ser gly leu asp leu leu phe cat cca gct act gca aaa cct ttg tca tgg caa cat tca aag att att 2665 cag gca ttc atg agt cag ggc gag cac aga caa gcc ctc aga tat att 2713 gln ala phe met ser gln gly glu his arg gln ala leu arg tyr ile cag aca atg aag cca aca gtg tcc agt ggt aac gat gtt atc ctt cac 2761 gln thr met lys pro thr val ser ser gly asn asp val ile leu his ctc act gtt ttg ctt ttt aat agg tgt atg gtt gaa gcc tgg aat ttt 2809 leu thr val leu leu phe asn arg cys met val glu ala trp asn phe ttg cgg caa cat tgc aat agg ttg aat ata gag gag tta ctg aag cac 2857 atg tat gaa gtc tgt cag gaa atg ggc ttg atg gaa gat tta ctg aag 2905 tta cca ttt aca gac act gag cag gaa tgt tta gtg aaa ttt ttg cag 2953 tcc agt gcc agc gtt cag aat cat gaa ttc ctt tta gtg cac cat ttg 3001 cag cgt gcc aat tat gtg cct gcc ttg aag ctg aac caa act ctg aag 3049 att aat gtt atg aat gat cgt gat cct cgt ttg cgg gag aga tca ctg 3097 gct cga aat tct ata tta gac cag tat gga aaa atc ctt cct aga gtc 3145 ala arg asn ser ile leu asp gln tyr gly lys ile leu pro arg val cat cga aaa tta gcc att gaa cga gct aag cct tat cat ctg tca aca 3193 his arg lys leu ala ile glu arg ala lys pro tyr his leu ser thr tca tca gtt ttt cga tta gtt tct aga ccc aaa cca tta tca gca gtt 3241 cca aag caa gtt gta aca gga act gtg ttg aca aga tct gtt ttc atc 3289 pro lys gln val val thr gly thr val leu thr arg ser val phe ile aac aat gtg tta tct aaa att gga gaa gtt tgg gca agc aaa gaa cct 3337 asn asn val leu ser lys ile gly glu val trp ala ser lys glu pro ata aat agc acc aca cct ttc aat agt tct aaa ata gaa gaa cca tct 3385 cct ata gtg tat tcg ctc cca gct cca gag ctg cct gag gca ttt ttt 3433 gga aca cca att tca aaa gca tca caa aaa att tct aga ctg cta gat 3481 gly thr pro ile ser lys ala ser gln lys ile ser arg leu leu asp ttg gtt gtt cag cct gtc ccc cgg cct tct cag tgt tcg gag ttt att 3529 cag caa agc tcc atg aaa tct cct ttg tac cta gta tcc cgt tca ctg 3577 ccc tca agt tcg caa tta aaa gga tcg cct cag gcc atc tcc agg gct 3625 tca gaa tta cat ttg ctt gaa act cct ctt gta gtt aag aaa gct aaa 3673 agt ttg gcc atg tca gtt act act tct gga ttt tct gag ttc act cct 3721 cag tcc atc ctg agg tct act cct cga tca aca cct tta gca tct ccc 3769 tct cca tca cct gga agg tct cct caa cga ctt aaa gaa act aga att 3817 tca ttt gtg gaa gaa gat gtc cac cca aaa tgg att cct ggg gct gca 3865 ser phe val glu glu asp val his pro lys trp ile pro gly ala ala gat gat agc aaa tta gaa gta ttt act aca cct aaa aaa tgt gca gtt 3913 asp asp ser lys leu glu val phe thr thr pro lys lys cys ala val cca gtg gaa act gaa tgg ccg aag agc aaa gat agg acc aca tct ttt 3961 ttc ctg aac agc cct gaa aag gag cat caa gaa atg gat gag ggg tca 4009 phe leu asn ser pro glu lys glu his gln glu met asp glu gly ser caa agt tta gag aaa ctg gat gtg agc aaa gga aac agc agt gtt tca 4057 atc aca tcc gat gag act acc tta gag tat cag gat gca ccg tca ccg 4105 gaa gac ctt gaa gag act gtt ttc acg gcc tct aag ccc aaa agc tct 4153 tcc act gca cta act act aat gta act gaa caa act gaa aag gat gga 4201 ser thr ala leu thr thr asn val thr glu gln thr glu lys asp gly gat aaa gat gta ttt gca tca gaa gta act cct tca gac cta cag aaa 4249 asp lys asp val phe ala ser glu val thr pro ser asp leu gln lys caa atg ggc aat tta gaa gat gca gaa aca aag gat ctc tta gtt gca 4297 gln met gly asn leu glu asp ala glu thr lys asp leu leu val ala gca gag gca ttt tca gaa ttg aat cac tta agc ccg gtt caa gga act 4345 ala glu ala phe ser glu leu asn his leu ser pro val gln gly thr gaa gct tct ctt tgt gca cca tca gtc tat gaa ggg aaa atc ttc acc 4393 glu ala ser leu cys ala pro ser val tyr glu gly lys ile phe thr cag aag tcc aag gta cca gtg ttg gac gaa gga tta aca tct gtt gaa 4441 acc tac acc cct gca att aga gca aat gac aat aaa tct atg gct gat 4489 thr tyr thr pro ala ile arg ala asn asp asn lys ser met ala asp gtc ctt ggt gat ggt gga aac tcc tcg ctc act atc tct gaa ggt cct 4537 att gtc tct gag cgc agg ctt aac cag gaa gta gcg ctg aac tta aaa 4585 gaa gat cat gaa gta gaa gtt ggt gta cta aaa gaa agt gtt gac tta 4633 cca gaa gaa aag ctt cca att tct gac agc cct cct gat act caa gaa 4681 att cat gtg att gaa caa gaa aag ctt gaa gct caa gat tca gga gaa 4729 ile his val ile glu gln glu lys leu glu ala gln asp ser gly glu gag gct agg aat ctt tca ttt aat gag tta tat ccc tct gga aca ctt 4777 glu ala arg asn leu ser phe asn glu leu tyr pro ser gly thr leu aag ctt cag tac aat ttt gat act att gac caa cag ttt tgt gac tta 4825 gct gat aac aaa gac act gct gaa tgt gac att gct gaa gta gat ggg 4873 gaa ctt ttt gtg gct caa agc aac ttt acc ttg ata ttg gaa ggt gaa 4921 glu leu phe val ala gln ser asn phe thr leu ile leu glu gly glu gaa gga gaa gtt gag cca ggt gat ttt gca tca tct gat gtg tta cct 4969 aaa gca gct aac aca gca act gaa gaa aaa ctt gta tgc agt ggg gaa 5017 aat gat aat cat gga caa att gca aat ttg cca tct gcc gta act agt 5065 asn asp asn his gly gln ile ala asn leu pro ser ala val thr ser gac caa aag tcc caa aaa gta gac act tta cca tat gtg cct gaa cct 5113 att aaa gta gca att gca gaa aat tta cta gat gta att aaa gac aca 5161 aga agt aaa gaa att act tca gat aca atg gaa cag tcc att cat gaa 5209 aca ata cct tta gtg agc caa aac ata atg tgt ccc act aaa ttg gtc 5257 thr ile pro leu val ser gln asn ile met cys pro thr lys leu val aaa tct gca ttt aag act gct cag gaa aca agc aca atg act atg aat 5305 gtc agc cag gtt gat gac gtg gtt tcc tcc aaa act cgt acg aga ggt 5353 caa cgt atc caa aac gtg aat gtc aaa tca gca caa cag gaa gca tca 5401 gca gat gtt gct act cct aag atg cca ggg cag tca gtc agg aag aaa 5449 ala asp val ala thr pro lys met pro gly gln ser val arg lys lys act agg aag gca aaa gaa att tct gaa gct tct gaa aac atc tat tct 5497 gat gtc aga gga cta ttt cag aac cag caa ata cct caa aat tct gtt 5545 asp val arg gly leu phe gln asn gln gln ile pro gln asn ser val acg cct agg aga gga agg aga aag aaa gaa gtt aat cag gac ata cta 5593 thr pro arg arg gly arg arg lys lys glu val asn gln asp ile leu gaa aac acc agt tct gtg gaa caa gaa tta 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val asp val gly lys pro ala leu gly lys ser ile tta gtg cca aac gag gaa ctt tcg atg gtg atg agc tct aag aaa aaa 6265 ctt aca aaa aag act gaa agt caa agc caa aaa cgt tca ttg cac tca 6313 gta tca gaa gaa cgc aca gat gaa atg aca cat aaa gaa aca aat gag 6361 cag gaa gaa aga ttg ctc gcc aca gct tcc ttc act aaa tca tcc cgc 6409 agc agc agg act cgg tct agc aag gcc atc ttg ttg ccg gac ctt tct 6457 gaa cca aac aat gag cct tta ttt tct cca gcg tca gaa gtt cca agg 6505 aaa gca aaa gct aaa aaa ata gag gtt cct gca cag ctg aaa gaa tta 6553 gtt tcg gat tta tct tct cag ttt gtc atc tca cct cct gct tta agg 6601 agc aga caa aaa aac aca tcc aat aag aac aag ctt gaa gat gaa ctg 6649 aaa gat gat gca caa tca gta gaa act ctg gga aag cca aaa gcg aaa 6697 lys asp asp ala gln ser val glu thr leu gly lys pro lys ala lys cga atc agg acg tca aaa aca aaa caa gca agc aaa aac aca gaa aaa 6745 gaa agt gct tgg tca ctt cct ccc ata gaa att cgg ctg att tcc ccc 6793 ttg gct agc cca gct gac gga gtc aag agc aaa cca aga aaa act aca 6841 gaa gtg aca gga aca ggt ctt gga agg aac aga aag aaa ctg tct tcc 6889 tat cca aag caa att tta cgc aga aaa atg ctg taatttcttg ggaagatttt 6942 met arg asp leu arg ala gln val thr ser gly leu leu pro phe pro ala cys gly pro gln leu glu val val asn ser ile thr gly glu arg leu ser ala tyr arg phe ser gly val asn glu gln pro pro val val leu ala val lys glu phe ser trp gln lys arg thr gly leu leu ile leu his pro ser leu arg trp leu phe gly val ala ala val val thr cys asn gln asn glu val glu ala ser asp leu glu val leu thr gly arg his leu cys phe gln leu val ser pro thr gly thr ala val ser thr leu ser tyr ile ser arg thr asn gln leu ala ala gly phe ser asp gly tyr leu ala leu trp asn met lys ser met lys arg glu tyr tyr ile gln leu glu ser gly gln val pro val tyr ala val thr phe gln glu pro glu asn asp arg arg asn cys cys tyr leu trp ala val gln leu ala phe gly asn arg lys cys leu ala ser gly gln ile leu gly gly met phe pro leu arg gly gln thr ser asn thr lys leu leu gly cys gln ser ile 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gly ser met arg asp leu arg ala gln val thr ser gly leu leu cca ttt cca gaa gtg act ctt caa gcc ctt gga gaa gac gaa ata aca 211 pro phe pro glu val thr leu gln ala leu gly glu asp glu ile thr tta gaa tct gtg ctt cgt gga aag ttt gct gcg ggg aaa aat gga ctt 259 gct tgc ttg gct tgt ggt cca caa ctt gag gta gta aac tct ata aca 307 ala cys leu ala cys gly pro gln leu glu val val asn ser ile thr gga gag cga ttg tct gct tac aga ttc agt gga gtc aat gaa cag cct 355 gly glu arg leu ser ala tyr arg phe ser gly val asn glu gln pro cct gta gtt tta gct gtg aaa gaa ttc tct tgg cag aag aga act gga 403 pro val val leu ala val lys glu phe ser trp gln lys arg thr gly tta tta ata gga ttg gaa gaa aca gaa ggg agt gtt ctc tgt ctt tat 451 gac ctt gga ata tca aaa gta gtt aaa gca gtt gtt ctt cct gga agg 499 gta aca gct att gaa cct ata att aat cat gga gga gcc agt gca agc 547 act cag cat tta cat cca agt ctg cga tgg ctt ttt gga gtg gca gct 595 thr gln his leu his pro ser leu arg trp leu phe gly val ala ala gtg gtc act gat gtt gga cag atc ctt ctt att gac cta tgt ttg gat 643 gac ttg tca tgc aat caa aat gaa gtt gaa gca tca gat ctt gaa gtt 691 cta act ggt atc cca gct gaa gta cca cac att aga gaa agt gtg atg 739 leu thr gly ile pro ala glu val pro his ile arg glu ser val met aga gaa ggg cgc cat ctg tgt ttc cag tta gta agt cca aca gga aca 787 arg glu gly arg his leu cys phe gln leu val ser pro thr gly thr gcc gtt tca act ctt agt tac ata agc agg aca aat cag ctt gct gca 835 ggt ttt tct gat ggc tat cta gca ctt tgg aac atg aaa agc atg aaa 883 gly phe ser asp gly tyr leu ala leu trp asn met lys ser met lys aga gaa tat tac ata caa ttg gaa agt gga caa gtt cct gta tat gct 931 arg glu tyr tyr ile gln leu glu ser gly gln val pro val tyr ala gtc act ttt caa gaa cct gag aat gat cgt cgg aat tgc tgc tac ttg 979 val thr phe gln glu pro glu asn asp arg arg asn cys cys tyr leu tgg gct gtt cag tct aca caa gat agt gaa ggg gat gtt ttg agt ttg 1027 cat ctg ctg cag ctg gcc ttt ggt aat aga aag tgt ttg gca tca gga 1075 his leu leu gln leu ala phe gly asn arg lys cys leu ala ser gly caa atc tta tat gag ggg tta gaa tac tgt gaa gaa aga tac acc ctg 1123 gac ctg aca ggt ggc atg ttc cct ttg agg gga cag acg agt aat acc 1171 asp leu thr gly gly met phe pro leu arg gly gln thr ser asn thr aaa ttg ttg gga tgc cag agt ata gag aaa ttt cga tct cat ggt gac 1219 lys leu leu gly cys gln ser ile glu lys phe arg ser his gly asp agg gag gaa ggc gtg aat gaa gct cta tcg cct gac act agt gtt tca 1267 arg glu glu gly val asn glu ala leu ser pro asp thr ser val ser gtc ttt acc tgg cag gtg aat ata tat gga cag gga aag cct tct gta 1315 val phe thr trp gln val asn ile tyr gly gln gly lys pro ser val tat ttg ggg ctt ttt gat ata aat cgt tgg tat cat gca caa atg cca 1363 tyr leu gly leu phe asp ile asn arg trp tyr his ala gln met pro gat tcg tta agg tca gga gaa tat cta cat aat tgc tct tat ttt gca 1411 asp ser leu arg ser gly glu tyr leu his asn cys ser tyr phe ala ctg tgg tca ttg gag tct gtt gta agt agg act tct cca cat ggc atc 1459 leu trp ser leu glu ser val val ser arg thr ser pro his gly ile ttg gat ata tta gta cat gag aga agt tta aat aga gga gtc cct cct 1507 leu asp ile leu val his glu arg ser leu asn arg gly val pro pro tca tat cca cct ccc gag cag ttt ttt aat cca agc act tat aat ttt 1555 gat gcc act tgt ttg tta aac tcg gga gtt gtt cat tta act tgt act 1603 ggc ttt cag aag gag act ttg act ttt tta aag aaa tca ggt cca tca 1651 ctc aat gaa ctc att cct gat ggt tat aat cga tgt ctt gta gct ggc 1699 leu asn glu leu ile pro asp gly tyr asn arg cys leu val ala gly ctt ctt tcc cca aga ttt gtt gat gtt cag cct tcc agt tta agc caa 1747 gaa gaa cag tta gaa gct ata ttg tca gca gca att cag act agt tcc 1795 ctg gga ctt ttg act ggt tat atc cga aga tgg ata aca gaa gaa caa 1843 cca aat tct gcc act aat ttg cgc ttt gtt ctt gaa tgg acg tgg aat 1891 pro asn ser ala thr asn leu arg phe val leu glu trp thr trp asn aaa gtg gtt ctc aca aaa gag gaa ttt gac aga cta tgt gtg cca tta 1939 ttt gat ggt tcg tgt cat ttc atg gat cca caa act ata cag tct atc 1987 phe asp gly ser cys his phe met asp pro gln thr ile gln ser ile cag caa tgc tat ttg ctt ctt agc aat ctt aat ata gtc ttg agc tgt 2035 ttt gca tca gaa gcc cga gag atc gct gag aga gga ctg ata gac tta 2083 agc aat aag ttt gtg gtt tcc cac ctc atc tgt cag tat gca caa gtg 2131 ser asn lys phe val val ser his leu ile cys gln tyr ala gln val gtt ctt tgg ttc tct cat tct ggg ctt tta cca gaa ggc ata gat gat 2179 val leu trp phe ser his ser gly leu leu pro glu gly ile asp asp tct gtg cag ttg tca agg tta tgc tac aac tac cct gta att cag aac 2227 tac tac acc agt cgt cga cag aag ttt gag cgt tta tca aga ggg aag 2275 tgg aat ccc gat tgc ttg atg att gat gga ctg gtt tct cag tta gga 2323 trp asn pro asp cys leu met ile asp gly leu val ser gln leu gly gag cga att gag aag ttg tgg aaa cga gat gaa gga ggc aca gga aaa 2371 tat cct cct gct agt ctg cat gca gta ctt gat atg tac cta tta gac 2419 ggc gtt act gaa gca gcc aaa cac tct att acc att tat ttg cta ctt 2467 gly val thr glu ala ala lys his ser ile thr ile tyr leu leu leu gat att atg tat tcc ttt ccc aac aaa aca gac act ccc att gaa tct 2515 asp ile met tyr ser phe pro asn lys thr asp thr pro ile glu ser ttc cca act gta ttt gcc att tct tgg ggc caa gtt aaa ctt att cag 2563 phe pro thr val phe ala ile ser trp gly gln val lys leu ile gln ggg ttt tgg ttg ata gat cat aat gac tat gag agt ggt ttg gat ctt 2611 gly phe trp leu ile asp his asn asp tyr glu ser gly leu asp leu ttg ttt cat cca gct act gca aaa cct ttg tca tgg caa cat tca aag 2659 att att cag gca ttc atg agt cag ggc gag cac aga caa gcc ctc aga 2707 ile ile gln ala phe met ser gln gly glu his arg gln ala leu arg tat att cag aca atg aag cca aca gtg tcc agt ggt aac gat gtt atc 2755 tyr ile gln thr met lys pro thr val ser ser gly asn asp val ile ctt cac ctc act gtt ttg ctt ttt aat agg tgt atg gtt gaa gcc tgg 2803 leu his leu thr val leu leu phe asn arg cys met val glu ala trp aat ttt ttg cgg caa cat tgc aat agg ttg aat ata gag gag tta ctg 2851 aag cac atg tat gaa gtc tgt cag gaa atg ggc ttg atg gaa gat tta 2899 lys his met tyr glu val cys gln glu met gly leu met glu asp leu ctg aag tta cca ttt aca gac act gag cag gaa tgt tta gtg aaa ttt 2947 ttg cag tcc agt gcc agc gtt cag aat cat gaa ttc ctt tta gtg cac 2995 cat ttg cag cgt gcc aat tat gtg cct gcc ttg aag ctg aac caa act 3043 his leu gln arg ala asn tyr val pro ala leu lys leu asn gln thr ctg aag att aat gtt atg aat gat cgt gat cct cgt ttg cgg gag aga 3091 tca ctg gct cga aat tct ata tta gac cag tat gga aaa atc ctt cct 3139 ser leu ala arg asn ser ile leu asp gln tyr gly lys ile leu pro aga gtc cat cga aaa tta gcc att gaa cga gct aag cct tat cat ctg 3187 tca aca tca tca gtt ttt cga tta gtt tct aga ccc aaa cca tta tca 3235 gca gtt cca aag caa gtt gta aca gga act gtg ttg aca aga tct gtt 3283 ttc atc aac aat gtg tta tct aaa att gga gaa gtt tgg gca agc aaa 3331 phe ile asn asn val leu ser lys ile gly glu val trp ala ser lys gaa cct ata aat agc acc aca cct ttc aat agt tct aaa ata gaa gaa 3379 cca tct cct ata gtg tat tcg ctc cca gct cca gag ctg cct gag gca 3427 ttt ttt gga aca cca att tca aaa gca tca caa aaa att tct aga ctg 3475 phe phe gly thr pro ile ser lys ala ser gln lys ile ser arg leu cta gat ttg gtt gtt cag cct gtc ccc cgg cct tct cag tgt tcg gag 3523 ttt att cag caa agc tcc atg aaa tct cct ttg tac cta gta tcc cgt 3571 phe ile gln gln ser ser met lys ser pro leu tyr leu val ser arg tca ctg ccc tca agt tcg caa tta aaa gga tcg cct cag gcc atc tcc 3619 agg gct tca gaa tta cat ttg ctt gaa act cct ctt gta gtt aag aaa 3667 gct aaa agt ttg gcc atg tca gtt act act tct gga ttt tct gag ttc 3715 act cct cag tcc atc ctg agg tct act cct cga tca aca cct tta gca 3763 tct ccc tct cca tca cct gga agg tct cct caa cga ctt aaa gaa act 3811 aga att tca ttt gtg gaa gaa gat gtc cac cca aaa tgg att cct ggg 3859 arg ile ser phe val glu glu asp val his pro lys trp ile pro gly gct gca gat gat agc aaa tta gaa gta ttt act aca cct aaa aaa tgt 3907 ala ala asp asp ser lys leu glu val phe thr thr pro lys lys cys gca gtt cca gtg gaa act gaa tgg ccg aag agc aaa gat agg acc aca 3955 tct ttt ttc ctg aac agc cct gaa aag gag cat caa gaa atg gat gag 4003 ser phe phe leu asn ser pro glu lys glu his gln glu met asp glu ggg tca caa agt tta gag aaa ctg gat gtg agc aaa gga aac agc agt 4051 gtt tca atc aca tcc gat gag act acc tta gag tat cag gat gca ccg 4099 val ser ile thr ser asp glu thr thr leu glu tyr gln asp ala pro tca ccg gaa gac ctt gaa gag act gtt ttc acg gcc tct aag ccc aaa 4147 agc tct tcc act gca cta act act aat gta act gaa caa act gaa aag 4195 gat gga gat aaa gat gta ttt gca tca gaa gta act cct tca gac cta 4243 asp gly asp lys asp val phe ala ser glu val thr pro ser asp leu cag aaa caa atg ggc aat tta gaa gat gca gaa aca aag gat ctc tta 4291 gtt gca gca gag gca ttt tca gaa ttg aat cac tta agc ccg gtt caa 4339 gga act gaa gct tct ctt tgt gca cca tca gtc tat gaa ggg aaa atc 4387 gly thr glu ala ser leu cys ala pro ser val tyr glu gly lys ile ttc acc cag aag tcc aag gta cca gtg ttg gac gaa gga tta aca tct 4435 phe thr gln lys ser lys val pro val leu asp glu gly leu thr ser gtt gaa acc tac acc cct gca att aga gca aat gac aat aaa tct atg 4483 val glu thr tyr thr pro ala ile arg ala asn asp asn lys ser met gct gat gtc ctt ggt gat ggt gga aac tcc tcg ctc act atc tct gaa 4531 ggt cct att gtc tct gag cgc agg ctt aac cag gaa gta gcg ctg aac 4579 gly pro ile val ser glu arg arg leu asn gln glu val ala leu asn tta aaa gaa gat cat gaa gta gaa gtt ggt gta cta aaa gaa agt gtt 4627 gac tta cca gaa gaa aag ctt cca att tct gac agc cct cct gat act 4675 caa gaa att cat gtg att gaa caa gaa aag ctt gaa gct caa gat tca 4723 gga gaa gag gct agg aat ctt tca ttt aat gag tta tat ccc tct gga 4771 aca ctt aag ctt cag tac aat ttt gat act att gac caa cag ttt tgt 4819 gac tta gct gat aac aaa gac act gct gaa tgt gac att gct gaa gta 4867 gat ggg gaa ctt ttt gtg gct caa agc aac ttt acc ttg ata ttg gaa 4915 asp gly glu leu phe val ala gln ser asn phe thr leu ile leu glu ggt gaa gaa gga gaa gtt gag cca ggt gat ttt gca tca tct gat gtg 4963 tta cct aaa gca gct aac aca gca act gaa gaa aaa ctt gta tgc agt 5011 ggg gaa aat gat aat cat gga caa att gca aat ttg cca tct gcc gta 5059 gly glu asn asp asn his gly gln ile ala asn leu pro ser ala val act agt gac caa aag tcc caa aaa gta gac act tta cca tat gtg cct 5107 gaa cct att aaa gta gca att gca gaa aat tta cta gat gta att aaa 5155 gac aca aga agt aaa gaa att act tca gat aca atg gaa cag tcc att 5203 cat gaa aca ata cct tta gtg agc caa aac ata atg tgt ccc act aaa 5251 his glu thr ile pro leu val ser gln asn ile met cys pro thr lys ttg gtc aaa tct gca ttt aag act gct cag gaa aca agc aca atg act 5299 atg aat gtc agc cag gtt gat gac gtg gtt tcc tcc aaa act cgt acg 5347 aga ggt caa cgt atc caa aac gtg aat gtc aaa tca gca caa cag gaa 5395 gca tca gca gat gtt gct act cct aag atg cca ggg cag tca gtc agg 5443 ala ser ala asp val ala thr pro lys met pro gly gln ser val arg aag aaa act agg aag gca aaa gaa att tct gaa gct tct gaa aac atc 5491 tat tct gat gtc aga gga cta ttt cag aac cag caa ata cct caa aat 5539 tyr ser asp val arg gly leu phe gln asn gln gln ile pro gln asn tct gtt acg cct agg aga gga agg aga aag aaa gaa gtt aat cag gac 5587 ser val thr pro arg arg gly arg arg lys lys glu val asn gln asp ata cta gaa aac acc agt tct gtg gaa caa gaa tta cag atc act aca 5635 ggt agg gaa tca aaa aga tta aaa tca tct cag ctg ttg gaa cca gca 5683 agg act cct aga aga att aaa aga tct gta gaa aat cag gaa agt gtt 5779 gaa att ata aat gat cta aaa gtt agt acg gta aca agt cct agc aga 5827 glu ile ile asn asp leu lys val ser thr val thr ser pro ser arg atg atc aga aaa ttg aga agt act aat tta gat gct tct gaa aat aca 5875 met ile arg lys leu arg ser thr asn leu asp ala ser glu asn thr gga aat aag caa gat gat aaa tcc agt gac aag cag ctg cgt att aaa 5923 cat gtt aga agg gtc aga ggg aga gaa gtt agt cca tca gat gtg aga 5971 gaa gac tcc aac ctt gag tca tct cag ttg act gtt caa gca gaa ttt 6019 gat atg tct gcc ata cct aga aaa cgt ggt aga cca aga aaa atc aat 6067 cca tct gaa gat gta gga tct aag gct gtt aag gaa gag aga agc ccc 6115 aag aag aaa gaa gct ccc agc att aga agg aga tct aca aga aat acc 6163 cca gct aaa agt gaa aat gtt gat gtt gga aaa cca gct tta gga aaa 6211 tcc att tta gtg cca aac gag gaa ctt tcg atg gtg atg agc tct aag 6259 aaa aaa ctt aca aaa aag act gaa agt caa agc caa aaa cgt tca ttg 6307 cac tca gta tca gaa gaa cgc aca gat gaa atg aca cat aaa gaa aca 6355 aat gag cag gaa gaa aga ttg ctc gcc aca gct tcc ttc act aaa tca 6403 tcc cgc agc agc agg act cgg tct agc aag gcc atc ttg ttg ccg gac 6451 ctt tct gaa cca aac aat gag cct tta ttt tct cca gcg tca gaa gtt 6499 cca agg aaa gca aaa gct aaa aaa ata gag gtt cct gca cag ctg aaa 6547 gaa tta gtt tcg gat tta tct tct cag ttt gtc atc tca cct cct gct 6595 tta agg agc aga caa aaa aac aca tcc aat aag aac aag ctt gaa gat 6643 gaa ctg aaa gat gat gca caa tca gta gaa act ctg gga aag cca aaa 6691 glu leu lys asp asp ala gln ser val glu thr leu gly lys pro lys gcg aaa cga atc agg acg tca aaa aca aaa caa gca agc aaa aac aca 6739 gaa aaa gaa agt gct tgg tca ctt cct ccc ata gaa att cgg ctg att 6787 tcc ccc ttg gct agc cca gct gac gga gtc aag agc aaa cca aga aaa 6835 act aca gaa gtg aca gga aca ggt ctt gga agg aac aga aag aaa ctg 6883 tct tcc tat cca aag caa att tta cgc aga aaa atg ctg taatttcttg 6932