Patent Application: US-89061304-A

Abstract:
the present invention relates to a dry analytical element useful for the determination and quantification of high - density lipoprotein cholesterol that uses yeast lipase from candida rugosa , a lipase enzyme source that has cholesterol esterase activity , which preferentially reacts with the cholesterol esters of hdl over cholesterol esters of other lipoproteins .

Description:
in the direct hdlc dry slide format , a lipase enzyme with cholesterol esterase activity was discovered which showed selectivity for hdlc derived esters over non - hdlc lipoprotein - derived esters . several esterase and lipase sources were screened for hdlc selectivity , and the hdlc specific cholesterol esterase source was the lipase ii f / d ( e . c . 3 . 1 . 1 . 13 , candida rugosa ) produced by the genzyme corporation ( catalogue # 70 6551 - 01 ). the preferred format of the thin film element used in this evaluation is shown in example 1 , but the locations of the enzymes and other reactive ingredients may be placed in a variety of positions within the thin film element , and a variety of different materials may be used for the various layers . the hdlc selectivity of this enzyme source was demonstrated by comparing the performance of the candida rugosa lipase against seven other enzyme sources ( table 2 ) using two different means : comparison of the kinetic response from pure human hdl and pure human ldl test fluids , and patient accuracy comparisons with neat patient samples . the kinetics of the human ldl fluid at 150 mg / dl compared to the human hdl fluid at 150 mg / dl shows a much larger response for the non - specific hdlc esterase ( fig1 — porcine pancreas ceh ) than the response for the hdlc - specific hdlc esterase ( fig2 — candida rugosa lipase ). similar non - selectivity was observed with the other esterase and lipase sources summarized in table 2 . the data show that the esterase and lipase sources screened in the hdlc dry slide format have more ldlc reactivity compared to hdlc reactivity than the candida rugosa lipase enzyme . dry analytical elements and their use are described in numerous publications , including u . s . pat . nos . 4 , 123 , 528 ; 4 , 786 , 605 ; 3 , 992 , 158 ; 4 , 258 , 001 ; 4 , 670 , 381 ; and european patent application nos . 051 183 ; 066 648 . the layers of the element of the present invention can be self - supporting , but preferably , these layers are disposed on a suitable dimensionally stable , chemically inert support . a support of choice should be compatible with the intended mode of detection . useful support material include but are not limited to paper , metal , foils , polystyrenes , polyesters , polycarbonates , and cellulose esters . in at least one of the layers of the element of this invention is a dye which is capable of reacting with an enzyme to form a color . in a preferred embodiment of this invention , a leuco dye is used that can react with hydrogen peroxide to form a color . the color can be detected optically by the naked eye , by a photodiode selected to respond to a particular wavelength of light , or by other optical detection systems known by those skilled in the art using absorption , reflectance , or fluorescence spectroscopy . in a preferred embodiment of this invention , a reflectometer is used to detect and quantitate the dye color . the element of this invention can include a wide variety of additives in appropriate layers as are known in the art to aid in manufacture , fluid spreading , and absorption and unwanted radiation . the element of the present invention can be prepared using conventional coating procedures and equipment as are described in the art including gravure , curtain , hopper , and other coating techniques . the element can be configured in a variety of forms , including elongated tapes of any desired width , sheets , slides or chips . the process can be manual or automated . a “ sample ” as used herein , refers to any substance that may contain the analyte of interest . a sample can be biological fluid , such as whole blood or whole blood components including red blood cells , white blood cells , platelets , serum and plasma , ascites , urine , cerebrospinal fluid , and other constituents of the body which may contain the analyte of interest . optionally , samples may be obtained from water , soil , and vegetation . the effectiveness and advantages of the invention are further illustrated by the following examples . the examples are meant to illustrate , but not to limit , the scope and spirit of the invention . example of a multilayer thin film element containing the hdlc - specific cholesterol esterase cholesterol esterase enzyme sources screened in the thin film element . the term “ e . c .” as used in table 1 enzyme code . it is based on the recommendations of the nomenclature committee of the international union of biochemistry and molecular biology ( iubmb ) and it describes each type of characterized enzyme for which an ec ( enzyme commission ) number has been provided . hdlc selectivity of the candida rugosa lipase and other esterase and lipase enzymes screened ( normalized to the candida rugosa lipase ; lower number signifies decreased selectivity ) are shown in table 2 . the candida rugosa lipase is considerably more selective for hdlc lipoproteins when judged with the human hdl and ldl test fluids , and has unique specificity characteristics not seen with other enzymes used in the well known cholesterol enzymatic cascade . the results for the patient accuracy testing show a similar trend as the human hdl and ldl test fluids for the hdlc selectivity . the correlation for the non - specific porcine pancreas esterase is considerably worse than the candida rugosa lipase . the lack of hdlc selectivity results in a very flat slope in the correlation plot and displays a large amount of scatter with the data . with the candida rugosa lipase in the dry slide , the correlation to the vitros magnetic hdlc precipitation method is far superior to the porcine pancreas esterase . the cross - reactivity to the non - hdl lipoprotein cholesterol causes the correlation to be very poor for the porcine pancreas esterase . because the candida rugosa lipase shows hdlc specificity , the cross - reactivity of the non - hdl lipoprotein cholesterol is greatly reduced and the hdlc accuracy is improved . the patient accuracy results for the other screened esterase and lipase sources yield a worse correlation than with the candida rugosa lipase . the correlation results for the enzyme sources are summarized in table 3 . these results confirm the conclusions reached from the human hdl and ldl test fluids that the candida rugosa lipase shows hdlc specificity that is not observed with other esterase and lipase sources . table 3 shows correlation results between the direct hdlc slide and the vitros magnetic hdlc precipitation method . the candida rugosa lipase hdlc specificity discovery improves the overall hdlc specificity of the dry slide element over other esterase and lipase sources . the hdlc specificity improvement is necessary because the currently known hdlc specific methods do not provide adequate hdlc specificity in this dry slide format . this additional increase in specificity in the enzyme cascade allows suitable performance in the dry slide element that would not be possible without this discovery . all cited materials herein are hereby incorporated by reference . accordingly , it should be noted that the present invention includes all modifications falling within the scope of the following claims . specifically , one skilled in the art would understand how to use candida rugosa lipase in a liquid - based assay system as well . 1 burstein , m ., scholnick , h . r ., & amp ; 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