Patent Application: US-201615099488-A

Abstract:
the present invention provides for a genetically modified host cell comprising a 2 - pyrrolidone synthase , or an enzymatically active fragment thereof , heterologous to the host cell .

Description:
before the invention is described in detail , it is to be understood that , unless otherwise indicated , this invention is not limited to particular sequences , expression vectors , enzymes , host microorganisms , or processes , as such may vary . it is also to be understood that the terminology used herein is for purposes of describing particular embodiments only , and is not intended to be limiting . in order to more fully appreciate the invention the following definitions are provided . as used in the specification and the appended claims , the singular forms “ a ,” “ an ,” and “ the ” include plural referents unless the context clearly dictates otherwise . thus , for example , reference to an “ expression vector ” includes a single expression vector as well as a plurality of expression vectors , either the same ( e . g ., the same operon ) or different ; reference to “ cell ” includes a single cell as well as a plurality of cells ; and the like . the terms “ optional ” or “ optionally ” as used herein mean that the subsequently described feature or structure may or may not be present , or that the subsequently described event or circumstance may or may not occur , and that the description includes instances where a particular feature or structure is present and instances where the feature or structure is absent , or instances where the event or circumstance occurs and instances where it does not . the terms “ host cell ” and “ host microorganism ” are used interchangeably herein to refer to a living biological cell that can be transformed via insertion of an expression vector . thus , a host organism or cell as described herein may be a prokaryotic organism ( e . g ., an organism of the kingdom eubacteria ) or a eukaryotic cell . as will be appreciated by one of ordinary skill in the art , a prokaryotic cell lacks a membrane - bound nucleus , while a eukaryotic cell has a membrane - bound nucleus . the term “ heterologous dna ” as used herein refers to a polymer of nucleic acids wherein at least one of the following is true : ( a ) the sequence of nucleic acids is foreign to ( i . e ., not naturally found in ) a given host microorganism ; ( b ) the sequence may be naturally found in a given host microorganism , but in an unnatural ( e . g ., greater than expected ) amount ; or ( c ) the sequence of nucleic acids comprises two or more subsequences that are not found in the same relationship to each other in nature . for example , regarding instance ( c ), a heterologous nucleic acid sequence that is recombinantly produced will have two or more sequences from unrelated genes arranged to make a new functional nucleic acid . specifically , the present invention describes the introduction of an expression vector into a host microorganism , wherein the expression vector contains a nucleic acid sequence coding for an enzyme that is not normally found in a host microorganism . with reference to the host microorganism &# 39 ; s genome , then , the nucleic acid sequence that codes for the enzyme is heterologous . the terms “ expression vector ” or “ vector ” refer to a compound and / or composition that transduces , transforms , or infects a host microorganism , thereby causing the cell to express nucleic acids and / or proteins other than those native to the cell , or in a manner not native to the cell . an “ expression vector ” contains a sequence of nucleic acids ( ordinarily rna or dna ) to be expressed by the host microorganism . optionally , the expression vector also comprises materials to aid in achieving entry of the nucleic acid into the host microorganism , such as a virus , liposome , protein coating , or the like . the expression vectors contemplated for use in the present invention include those into which a nucleic acid sequence can be inserted , along with any preferred or required operational elements . further , the expression vector must be one that can be transferred into a host microorganism and replicated therein . preferred expression vectors are plasmids , particularly those with restriction sites that have been well documented and that contain the operational elements preferred or required for transcription of the nucleic acid sequence . such plasmids , as well as other expression vectors , are well known to those of ordinary skill in the art . the term “ transduce ” as used herein refers to the transfer of a sequence of nucleic acids into a host microorganism or cell . only when the sequence of nucleic acids becomes stably replicated by the cell does the host microorganism or cell become “ transformed .” as will be appreciated by those of ordinary skill in the art , “ transformation ” may take place either by incorporation of the sequence of nucleic acids into the cellular genome , i . e ., chromosomal integration , or by extrachromosomal integration . in contrast , an expression vector , e . g ., a virus , is “ infective ” when it transduces a host microorganism , replicates , and ( without the benefit of any complementary virus or vector ) spreads progeny expression vectors , e . g ., viruses , of the same type as the original transducing expression vector to other microorganisms , wherein the progeny expression vectors possess the same ability to reproduce . the terms “ isolated ” or “ biologically pure ” refer to material that is substantially or essentially free of components that normally accompany it in its native state . as used herein , the terms “ nucleic acid sequence ,” “ sequence of nucleic acids ,” and variations thereof shall be generic to polydeoxyribonucleotides ( containing 2 - deoxy - d - ribose ), to polyribonucleotides ( containing d - ribose ), to any other type of polynucleotide that is an n - glycoside of a purine or pyrimidine base , and to other polymers containing nonnucleotidic backbones , provided that the polymers contain nucleobases in a configuration that allows for base pairing and base stacking , as found in dna and rna . thus , these terms include known types of nucleic acid sequence modifications , for example , substitution of one or more of the naturally occurring nucleotides with an analog ; intemucleotide modifications , such as , for example , those with uncharged linkages ( e . g ., methyl phosphonates , phosphotriesters , phosphoramidates , carbamates , etc . ), with negatively charged linkages ( e . g ., phosphorothioates , phosphorodithioates , etc . ), and with positively charged linkages ( e . g ., arninoalklyphosphoramidates , aminoalkylphosphotriesters ); those containing pendant moieties , such as , for example , proteins ( including nucleases , toxins , antibodies , signal peptides , poly - l - lysine , etc . ); those with intercalators ( e . g ., acridine , psoralen , etc . ); and those containing chelators ( e . g ., metals , radioactive metals , boron , oxidative metals , etc .). as used herein , the symbols for nucleotides and polynucleotides are those recommended by the iupac - iub commission of biochemical nomenclature ( biochem . 9 : 4022 , 1970 ). the term “ operably linked ” refers to a functional linkage between a nucleic acid expression control sequence ( such as a promoter ) and a second nucleic acid sequence , wherein the expression control sequence directs transcription of the nucleic acid corresponding to the second sequence . the enzymatically active fragment is any polypeptide capable of catalyzing reaction ( 1 ). the enzymatically active fragment is an enzyme that has an amino acid sequence that is at least 70 %, 75 %, 80 %, 85 %, 90 %, 95 % or 99 % identical to the amino acid sequence of seq id no : 1 . the enzymatically active fragment retains amino acids residues that are recognized as conserved for the enzyme . the enzymatically active fragment may have non - conserved amino acid residues replaced or found to be of a different amino acid , or amino acid ( s ) inserted or deleted , but which does not affect or has insignificant effect on the enzymatic activity of the enzymatically active fragment . the enzymatically active fragment may be found in nature or be an engineered mutant thereof . the enzymatically active fragment can comprise one or more of the following conserved amino acid sites / residues : acyl - activating enzyme ( aae ) consensus motif ( residues 137 , 140 - 145 , 147 - 148 ), acyl - activating enzyme ( aae ) consensus motif ( residues 140 , 257 - 258 , 279 - 284 , 357 , 369 , 372 , 382 , 458 ), amp binding site ( residues 140 , 180 - 181 , 227 , 229 - 230 , 233 , 257 - 258 , 279 - 284 , 357 , 369 , 372 , 379 - 382 , 439 ), and coa binding site ( residues 180 , 229 - 230 , 233 , 257 , 379 - 381 , 433 , 439 ). in some embodiments , the host cell is capable of synthesizing compound 1 or uptaking compound 1 from the environment or culture . in some embodiments , the host cell further comprises one or more enzymes of a pathway for synthesizing compound 1 from a carbon source . in some embodiments , the pathway for synthesizing compound 1 from a carbon source is native to the host cell . in some embodiments , the pathway for synthesizing compound 1 from a carbon source is heterologous to the host cell . in some embodiments , the carbon source is a carbon source the host cell in the wild - type form is capable of uptaking . in some embodiments , the host cell comprises a first one or more nucleic acids encoding the 2 - pyrrolidone synthase , or an enzymatically active fragment thereof , operably linked to a promoter capable of expressing the 2 - pyrrolidone synthase , or an enzymatically active fragment thereof , in the host cell . in some embodiments , the host cell comprises a second one or more nucleic acids encoding one or more enzymes of a pathway for synthesizing compound 1 from a carbon source , operably linked to a promoter capable of expressing the one or more enzymes of a pathway for synthesizing compound 1 from a carbon source in the host cell . in some embodiments , the first and / or second nucleic acids are stably integrated into a chromosome of the host cell . in some embodiments , the first and / or second nucleic acids are capable of stable introduction into the host cell . in some embodiments , the first and / or second nucleic acids are vectors , or expression vectors . in some embodiments , the first and / or second nucleic acids are the same nucleic acid . in some embodiments , the first and / or second nucleic acids are separate nucleic acids . in some embodiments , n is an integer from 1 to 20 . in some embodiments , n is an integer from 1 to 10 . in some embodiments , n is an integer from 1 to 9 . in some embodiments , n is an integer from 1 to 8 . in some embodiments , n is an integer from 1 to 7 . in some embodiments , n is an integer from 1 to 6 . in some embodiments , n is an integer from 1 to 7 . in some embodiments , n is an integer from 1 to 6 . in some embodiments , n is an integer from 1 to 5 . in some embodiments , n is an integer from 1 to 4 . in some embodiments , n is an integer from 1 to 3 . when n is 1 , compound 2 is butyrolactam ( 2 - pyrrolidone ). when n is 2 , compound 2 is valerolactam ( 2 - piperidinone ). when n is 3 , compound 2 is caprolactam . the host cell can be any eukaryotic cell , such as a yeast , or prokaryotic cell , such as a bacterium . in some embodiments , the host cell is yeast . yeast host cells suitable for practice of the methods of the invention include , but are not limited to , yarrowia , candida , bebaromyces , saccharomyces , schizosaccharomyces and pichia , including engineered strains provided by the invention . in one embodiment , saccharomyces cerevisae is the host cell . in one embodiment , the yeast host cell is a species of candida , including but not limited to c . tropicalis , c . maltosa , c . apicola , c . paratropicalis , c . albicans , c . cloacae , c . guillermondii , c . intermedia , c . lipolytica , c . panapsilosis , c . zeylenoides , and c . tropicalis . in some embodiments the host cell is a bacterium , such as a gram - positive or gram - negative cell . bacterial host cells suitable for practice of the methods of the invention include , but are not limited to , escherichia , bacillus , salmonella , klebsiella , enterobacter , pseudomonas , streptomyces , cynechocystis , cynechococcus , sinorhizobium , and caulobacter , including engineered strains provided by the invention . in some embodiments , the 2 - pyrrolidone synthase is heterologous to the cell . 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( 2009 ). “ towards prediction of metabolic products of polyketide synthases : an in silico analysis .” plos comput biol 5 ( 4 ): e1000351 . zazopoulos , e ., et al . ( 2003 ). “ a genomics - guided approach for discovering and expressing cryptic metabolic pathways .” nat biotechnol 21 ( 2 ): 187 - 190 . it is to be understood that , while the invention has been described in conjunction with the preferred specific embodiments thereof , the foregoing description is intended to illustrate and not limit the scope of the invention . other aspects , advantages , and modifications within the scope of the invention will be apparent to those skilled in the art to which the invention pertains . all patents , patent applications , and publications mentioned herein are hereby incorporated by reference in their entireties . the invention having been described , the following examples are offered to illustrate the subject invention by way of illustration , not by way of limitation . 2 - pyrrolidone is a “ top value - added chemical ” from biomass with myriad applications as a solvent , polymer precursor and active pharmaceutical intermediate . a novel 2 - pyrrolidone synthase from streptomyces aizunensis orf27 was identified by retro - biosynthetic analysis of polyketide natural products . orf27 catalyzes the atp dependent ring closing of γ - aminobutyrate to form 2 - pyrrolidone . although orf27 had the tendency to aggregate and lose activity , expression at 25 ° c . and fusing to the maltose binding protein increased its activity in vivo . recombinant escherichia coli was metabolically engineered for the production of 2 - pyrrolidone from glutamate by expressing both the genes encoding gadb , a glutamate decarboxylase , and orf27 . e . coli &# 39 ; s native caic , a betaine - coa ligase , was also discovered to be able to catalyze 2 - pyrrolidone formation from gaba , representing an unintended crosstalk during high flux microbial biosynthesis . a gadb mutant lacking his 465 and thr 466 , gadb_δht , has higher activity at intracellular ph than the native gadb , incorporation of which improved the efficiency of one - pot 2 - pyrrolidone biosynthesis in vivo . when the recombinant e . coli strain expressing the e . coli gadb_δht mutant , plus the streptomyces aizunensis orf27 mbp fusion was cultured in zym - 5052 medium containing 9 g / l of l - glutamate , 1 . 1 g / l of 2 - pyrrolidone was produced by converting 7 . 7 g / l of l - glutamate within 31 h , achieving 25 % conversion . we propose a two enzymatic step microbial process for 2 - pyrrolidone biosynthesis from glutamate : 1 ) decarboxylation of glutamate to form γ - aminobutyrate ( gaba ); 2 ) enzymatic ring closing of gaba into 2 - pyrrolidone ( fig1 b ). while the first enzymatic step is known ( le vo , kim et al . 2012 , ma , lu et al . 2012 , takahashi , shirakawa et al . 2012 , park , kim et al . 2013 , shi , jiang et al . 2013 ), the second step has not been demonstrated under mild fermentation conditions ( stavila and loos 2013 ). retrobiosynthetic analysis , a product - targeted design of biological pathways , has previously been applied to uncover the necessary enzymatic transformation of substrates ( moon 2009 , bachmann 2010 ). however , even the relatively comprehensive enzyme databases , such as brenda , cannot cover unprecedented enzymatic reactions on specific substrates , in this case 2 - pyrrolidone synthase ( chang , schomburg et al . 2015 ). poor prediction of enzyme substrate activities via bioinformatics or computation makes it difficult to identify the exact gene candidate from a huge list of protein homologs ( amin , erdin et al . 2013 ). therefore , significant trial and error process is usually involved during enzyme discovery . we employed a targeted strategy to identify appropriate enzyme candidates for the gaba activation step by conducting retro - biosynthetic analysis of polyketides . type i polyketide synthases are mega - synthases whose enzymatic domain organization predictably correlate with their natural product &# 39 ; s chemical structure — usually referred to as the colinearity rule ( du , sanchez et al . 2001 , menzella , reid et al . 2005 , weissman and muller 2008 , khosla , kapur et al . 2009 , li , roege et al . 2009 , yadav , gokhale et al . 2009 , du and lou 2010 , tran , broadhurst et al . 2010 , wong , chen et al . 2010 , dutta , whicher et al . 2014 ). while pks genes are responsible for biosynthesis of the characteristic polyketide aglycone backbone , auxiliary enzymes are involved in starter unit activation and / or post aglycone modification ( 2002 , ogasawara , katayama et al . 2004 , li , llewellyn et al . 2005 , simunovic , zapp et al . 2006 , llewellyn , li et al . 2007 , kalaitzis , cheng et al . 2009 , shinohara , kudo et al . 2011 ). the close positioning of pks genes with auxiliary enzymes in the same gene cluster makes it easy to pinpoint the substrate these auxiliary enzymes act on . here we report the discovery in streptomyces aizunensis of orf27 , an auxiliary enzyme in the linearmycin a biosynthetic cluster that performs the gaba activation step to form 2 - pyrrolidone under mild fermentation conditions . coupling this 2 - pyrrolidone synthase with glutamate decarboxylase , which forms gaba from glutamate , we achieved the first demonstration of the full 2 - pyrrolidone biosynthetic pathway in e . coli . therefore , we propose a two enzymatic step microbial process towards 2 - pyrrolidone biosynthesis from glutamate : 1 ) decarboxylation of glutamate to form γ - aminobutyrate ( gaba ); 2 ) activation of gaba &# 39 ; s acid group , allowing self - lactamization to form 2 - pyrrolidone ( fig1 b ). while the first enzymatic step is known , the second step has not been demonstrated at mild fermentation conditions . the enzyme database , such as brenda - enzymes suffers from incomplete coverage of unreported or even reported enzymatic reactions . poor prediction of enzyme substrate via bioinformatics or computation made it hard to identify the exact gene candidate from a huge list of protein homologs . therefore , significant trial and error is usually involved during enzyme discovery . we employed a targeted strategy to identify appropriate enzyme candidates for the gaba activation step by conducting retro - biosynthetic analysis of polyketides . type i polyketide synthases are mega - synthases whose enzymatic domain organization predictably correlate with their natural product &# 39 ; s chemical structure — usually referred to as the colinearity rule . while pks genes are responsible for biosynthesis of the characteristic polyketide aglycone backbone , auxiliary enzymes are involved in starter unit activation and / or post aglycone modification . the close positioning of pks genes with auxiliary enzymes in the same gene cluster makes it easy to pinpoint the substrate these auxiliary enzyme act on . here we report the discovery in streptomyces aizunensis of orf27 , an auxiliary enzyme in eco - 02301 biosynthetic cluster , that perform the gaba activation step under mild fermentation conditions . coupling this 2 - pyrrolidone synthase with glutamate decarboxylase that forms gaba , we achieved the first demonstration of the full 2 - pyrrolidone biosynthetic pathway in e . coli . literature review combined with pks databases , such as nrps - pks ( the webpage of : nii . res . in / nrps - pks . html ), mapsi ( http :// gate . smallsoft . co . kr : 8008 / pks / mapsidb /), norine ( http :// bioinfo . lifl . fr / norine /), dobiscuit ( the webpage of : bio . nite . go . jp / pks /), pkminer ( http :// pks . kaist . ac . kr / pkminer /) and clustermine360 ( the webpage of : clustermine360 . ca /), were explored to identify polyketides with both positively charged , amine - containing starter units and gene cluster sequence information . retrobiosynthetic analysis of polyketide natural products was performed to predict starter units that are gaba or its close analogs . sequence information was further analyzed for auxiliary enzyme orf annotation and function prediction . the hypothesized reaction candidates were reverse transcribed using the optimized e . coli codon usage using dna 2 . 0 &# 39 ; s algorithm and synthesized and prepared to be heterologously expressed in e . coli for characterization . e . coli strain dh10b [ f - mcra δ ( mrr - hsdrms - mcrbc ) φ80dlaczδm15 δlacx74 reca1 enda1 arad139 a ( ara , leu ) 7697 galu galk λ - rpsl ( strr ) nupg ] was used for all molecular biology manipulations . dh10b and bl21 star ( de3 ) [ f - ompt hsdsb ( rb - mb -) gal dcm rne131 ( de3 )] were used as hosts for production of 2 - pyrrolidone . competent cells of both strains were purchased from invitrogen corporation ( carlsbad , calif .). all the strains , plasmids , and oligonucleotides utilized in this study are listed in tables 1 - 3 . cultures were propagated in luria - bertani ( lb ) medium . lb ( miller ) medium was prepared from dehydrated powder according to the manufacturer &# 39 ; s instructions ( bd biosciences , san jose , calif .). nickel nitrilotriacetic acid agarose ( ni - nta ) resin and sds - page gels were purchased from qiagen and biorad , respectively . protein samples were concentrated using 10 kda mmco amicon ultra filters ( millipore ). dna and protein concentrations were determined using a nanodrop 1000 spectrophotometer ( thermo scientific ). for high - density shake flask cultures , studier &# 39 ; s autoinduction zym - 5052 medium was prepared according to the published protocol . chloramphenicol ( 25 μg / ml ), kanamycin ( 20 μg / ml ) and ampicillin ( 100 μg / ml ) were added where desired to provide selective pressure for plasmid maintenance . during 2 - pyrrolidone production , one - quarter of the antibiotic concentration was used : chloramphenicol ( 6 . 25 μg / ml ), kanamycin ( 5 μg / ml ) and ampicillin ( 25 μg / ml ). 2 - pyrrolidone , glutamic acid , gaba , and atp were purchased from sigma - aldrich ( st . louis , mich .). all molecular biology manipulations were performed according to standard practices ( sambrook and russell , 2001 ). genes encoding orf27 ( genbank : aax98201 . 1 ) and orf36 ( genbank : aax98210 . 1 ) were recoded using e . coli codon usage with biobrick overhangs 5 ′- gaattc aaa agatct aggaggcat - 3 ′ ( seq id no : 13 ) on the 5 ′ end and 5 ′- taa ggatcc aaa ctcgag - 3 ′ ( seq id no : 14 ) on the 3 ′ end . dna 2 . 0 ( menlo park , calif .) cloned the genes into plasmid vectors creating pdna2 . 0 - orf27 and pdna2 . 0 - orf36 , respectively . the genes encoding wild - type gadb and the variant gadb_δht , were amplified from e . coli mg1655 genomic dna using the primers specified . gadb_δht lacks histidine 465 and threonine 466 of e . coli gadb . the construction of each plasmid is described in table 4 . for expression and purification of orf27 , the orf27 gene was cloned into pet28b and the resulting plasmid , pet28b - n - orf27 , was transformed into e . coli bl21 star ( de3 ) for n - terminal 6 × his orf27 overexpression . the overnight culture was inoculated ( 1 : 100 v / v ) into 1 l lb medium containing 5 μg / ml kanamycin . the culture was grown at 37 ° c . until the o . d . reached 0 . 6 and cooled on ice for 20 min . 0 . 5 mm iptg was added to induce n - 6 × his orf27overexpression for 16 h at 18 ° c . the cells were harvested by centrifugation ( 8000 × g , 6 min , 4 ° c . ), resuspended in 30 ml of lysis buffer ( 50 mm hepes , ph 8 . 0 , 0 . 5 m nacl , and 10 mm imidazole ), and lysed by sonication on ice . cellular debris was removed by centrifugation ( 20 , 000 × g , 30 min , 4 ° c .). ni - nta agarose resin was added to the supernatant ( 1 ml / l of culture ), and the solution was rocked at 4 ° c . for 1 h . the protein resin mixture was loaded onto a gravity flow column , and proteins were washed with washing buffer ( 50 mm hepes , ph 8 . 0 , 0 . 5 m nacl , and 20 mm imidazole ) and eluted with elution buffer ( 50 mm hepes , ph 8 . 0 , 0 . 5 m nacl , and 250 mm imidazole ). purified proteins ( 60 mg from 1 l culture ) were concentrated to 280 mg / ml and buffer exchanged into storage buffer ( 50 mm hepes , ph 8 . 0 , 8 % glycerol ). the final proteins were aliquoted and flash frozen in liquid nitrogen and stored at − 80 ° c . c - terminal 6 × his orf27 ( 65 mg / l lb culture ) was produced using bl21 star ( de3 ) transformed with pet28b - c - orf27 , purified by ni - nta agarose resin and stocked at 220 mg / ml in storage buffer ( 50 mm hepes , ph 8 . 0 , 8 % glycerol ). liquid chromatography ( lc ) separation of 2 - pyrrolidone was conducted at 55 ° c . with an inertsil ods - 3 reverse - phase c18 column ( 250 mm length , 2 . 1 mm internal diameter , 3 μm particle size ; gl sciences ) using a 1100 series high - performance lc system ( agilent technologies ). the mobile phase was composed of 0 . 1 % formic acid in h 2 o ( solvent a ) and 0 . 1 % formic acid in meoh ( solvent b ). butyrolactam was separated with the following gradient : 40 % to 60 % b for 4 . 5 min , 60 % to 100 % b for 1 . 5 min , 100 % to 40 % b for 0 . 5 min , held at 10 % b for 8 . 5 min . a flow rate of 0 . 18 ml / min was used throughout . 2 . 5 . 2 . 1 time - of - flight mass spectrometry method for 2 - pyrrolidone , gaba dimer accurate mass monitoring the lc system was coupled to an agilent technologies 6210 electrospray time - of - flight ( tof ) mass spectrometer . nitrogen gas was used as both the nebulizing and drying gas to facilitate the production of gas - phase ions . the drying and nebulizing gases were set to 11 l / min and 25 psig , respectively , and a drying gas temperature of 320 ° c . was used throughout . esi was conducted in the positive - ion mode with a capillary voltage of 3 . 5 kv . mass measurements were carried out in the tof - scan monitoring mode for the detection of [ m + h ]+ ions ( 2 - pyrrolidone , m / z = 86 . 0600 ; gaba dimer , m / z = 189 . 1234 ). the instrument was tuned for a range of m / z 70 to 300 . data acquisition and processing were performed using chemstation ( agilent technologies ). 2 . 5 . 2 . 2 selected ion monitoring mass spectrometry method for 2 - pyrrolidone , gaba , glutamate quantification the lc system was coupled to an agilent technologies lc - msd sl electrospray ionization mass ( esi ms ) spectrometer . nitrogen gas was used as both the nebulizing and drying gas to facilitate the production of gas - phase ions . the drying and nebulizing gases were set to 10 l / min and 20 psig , respectively , and a drying gas temperature of 300 ° c . was used throughout . esi was conducted in the positive - ion mode with a capillary voltage of 4 kv . mass measurements were carried out in the selected ion monitoring ( sim ) mode ( 2 - pyrrolidone , m / z 86 ; gaba , m / z 104 ; glutamate , m / z 148 ) for the detection of [ m + h ]+ ions . data acquisition and processing were performed using agilent chemstation software ( agilent technologies ). to confirm the minimal system for orf27 - catalyzed 2 - pyrrolidone formation , a reaction mixture containing 5 μm of orf27 , 1 mm gaba substrates , 1 mm atp and 1 mm mg ( cl ) 2 in 100 mm hepes ( ph 7 . 5 ) and incubated at 25 ° c . for 30 min . the reactions were quenched by addition of methanol to a final concentration of 50 % ( v / v ), and the supernatant filtered through 10 k amicon ultra - 0 . 5 ml centrifugal filters ( millipore ). the filtered solution was analyzed using the lc - ms - tof method described above . control reactions were carried out without enzyme , without atp , or without mgcl 2 ( supplying additional 0 . 2 mm edta ). 600 μl of culture was cooled on ice and centrifuged at 18 , 000 × g for 5 min at 4 ° c . 250 μl of the supernatant was mixed with 250 μl methanol to a final concentration of 50 % ( v / v ), and the mixed solution filtered through 10 k amicon ultra - 0 . 5 ml centrifugal filters ( millipore ) by centrifuging at 20 , 000 × g for 15 min . the filtered solution was diluted into the respective linear range of detection for 2 - pyrrolidone and analyzed by lc - ms - tof . e . coli strains ( jz - 298 , jz - 171 , jz - 297 , jz - 299 , jz - 316 , jz - 317 , jz - 370 , jz - 393 ) carrying the proposed gaba activating enzyme expression plasmids ( orf27 , mbp - orf27 or caic ) were inoculated into 25 - ml lb medium with chloramphenicol ( 25 μg / ml ) and grown at 37 ° c . e . coli expressing rfp was utilized as a negative control ( jz - 172 ). when the o . d . reached around 0 . 5 , the culture was cooled to various temperatures ( 18 ° c .- 37 ° c .). 50 ng / ml of anhydrotetracycline ( atc ) was added for protein production and gaba was supplied to a final concentration of 0 - 10 mm . the 2 - pyrrolidone titer was analyzed 24 h after induction . e . coli strains ( jz - 336 ˜ jz - 353 , jz - 371 ˜ jz - 380 , jz - 386 ) carrying both glutamate decarboxylase and gaba activating enzyme expression plasmids were inoculated in 25 ml lb medium containing various concentrations of glutamate ( 0 to 9 g / l ) with appropriate antibiotics and grown at 37 ° c . when o . d . reached around 0 . 6 , the culture was cooled to 25 ° c . iptg and atet were added to a final concentration of 500 μm and 50 ng / ml , respectively to induce protein expression . the ph was titrated by adding 0 . 6n hcl solution , and the culture was placed in a 25 ° c . incubator . 2 - pyrrolidone titer was analyzed at 24 h after induction . e . coli strains ( jz - 344 , jz - 386 ) carrying both glutamate decarboxylase and gaba activating enzyme expression plasmids were inoculated into 10 ml of lb or lb plus 5 g / l glutamate overnight . on day 2 , the overnight culture was inoculated 1 : 100 ( v / v ) into 25 ml studier &# 39 ; s autoinduction zym - 5052 medium with various concentrations of glutamate ( 0 g / l to 9 g / l ) and appropriate antibiotics ( studier 2005 ). the culture was incubated at 37 ° c . when the o . d . reached around 0 . 6 , the culture was cooled to 25 ° c . the ph was titrated to 5 . 25 by adding 0 . 6n hcl solution . the culture was then placed at 25 ° c . incubator and 2 - pyrrolidone titer was analyzed at 24 h later . twenty four polyketides with amine containing polyketides were screened using retrobiosynthetic analysis : vecinistatin , 13 - desmethyl spirolide c , chejuenolides a and b , oxazolomycins , bacillaene , myxovirescin a , onnamides , virginiamycin m , kirromycin , pederin , etnangien , stellattamide - a , myxalamids , tolytoxin , salinilactam , mll - 449 , hitachimycin , leinamycin , lankacidin , indanomycin , be - 14106 , desertomycin a and b , eco - 0501 , linearmycin a and eco - 02301 . among them , desertomycin a , linearmycin a and eco - 02301 have a gaba - containing c4 amine starter unit , and linearmycin a has the biosynthetic pks gene cluster sequence information available ( fig8 ) ( sakuda , guce - bigol et al . 1996 , zazopoulos , huang et al . 2003 , james b . mcalpine 2005 , wenjun zhang 2010 ). two orfs in the gene cluster , orf27 and orf36 , were predicted to be amp - dependent synthetases ( bachmann 2008 ). orf36 was highly homologous to a previously identified 5 - aminolevulinyl - coa synthase responsible for 2 - amino - 3 - hydroxycyclopent - 2 - enone five membered ring formation during eco - 02301 biosynthesis ( wenjun zhang 2010 ). so orf27 is likely to be involved in activation of the gaba starter unit loading , which made it the best candidate for 2 - pyrrolidone synthase . both purified n - terminal 6 × his orf27 and c - terminal 6 × his orf27 were able to activate gaba &# 39 ; s carboxyl group and result in the formation of 2 - pyrrolidone ( fig2 a ). even though orf27 is predicted to be an acyl - coa ligase , coash is not required for 2 - pyrrolidone formation . the minimal reaction system constitutes gaba , enzyme orf27 , atp and mg 2 + ( fig2 b ). no 4 - aminobutyryl - coa was detected when coash &# 39 ; s was added to the reaction . mass ions corresponding to other off - pathway products , such as the gaba dimer , were also not observed . the ph profile of orf27 activity was determined , and the enzyme has a ph optimum of 8 . 0 . the enzyme precipitates and becomes inactive when the ph drops below 6 . 0 . 3 . 2 . 1 orf27 and e . coli &# 39 ; s native caic catalyze 2 - pyrrolidone formation in vivo e . coli jw2637 - 4 from the keio knockout library contains a knockout of gabt , which encodes a gaba transaminase involved in gaba catabolism ( baba , ara et al . 2006 ). this host was initially used to confirm production of 2 - pyrrolidone in vivo . e . coli jz - 298 ( orf27 ), and jz - 172 ( rfp negative control ) were grown in lb medium containing 0 , 1 , and 10 mm gaba ( fig3 ). at 0 mm and 1 mm gaba , 2 - pyrrolidone was observed only in the presence of orf27 . surprisingly , at 10 mm gaba , slight 2 - pyrrolidone production was observed in the rfp control strain , indicating that nonspecific catalysis of gaba by a native e . coli enzyme also contributes to 2 - pyrrolidone formation . various amp activating enzymes were cloned and overexpressed , among them caic overexpression lead to 2 - pyrrolidone formation ( bernal , arense et al . 2008 ). e . coli jw0036 - 1 , the keio collection caic deletion mutant , was transformed with pbbe2c - rfp , creating strain e . coli jz - 393 , which overexpresses rfp ; this strain was no longer able to produce 2 - pyrrolidone when fed 10 mm gaba ( fig3 ). 3 . 2 . 2 increased orf27 functional expression improves gaba to 2 - pyrrolidone conversion in vivo although both versions of the n - and c - terminal his - tagged orf27 were expressed solubly at 30 ° c ., orf27 had the tendency to aggregate and become insoluble one day after induction depending on the temperature ( fig9 a and 9b ). caic may have higher thermostability and can be functionally expressed at 37 ° c . by balancing reaction rate and enzyme functional expression , the optimal temperature was determined to be 25 ° c . for both enzymes to maximize 2 - pyrrolidone titer from gaba . the mbp fusion increased orf27 &# 39 ; s protein solubility and resulted in a 2 × improvement in overall titer ( fig4 ). this strategy did not prevent orf27 &# 39 ; s protein aggregation though . an mbp fusion with caic had a slight incremental impact on the 2 - pyrrolidone titer from gaba due to caic &# 39 ; s innate higher thermostability . to test whether mbp - orf27 overexpression may cause aggregation , thus toxicity for the cell , a low copy number plasmid harboring the sc101 origin of replication and mbp - orf27 was tested . however , use of this plasmid decreased the titer ( fig4 ), presumably due to lower expression of the enzyme . ch184 is an e . coli strain with slow ribosome translation rate ( t . ruusala 1984 ). previous studies showed that this is a good host for expression of multidomain proteins with poor solubilities , presumably a result of co - translational folding of multidomain proteins ( t . ruusala 1984 , fluitt , pienaar et al . 2007 , proshkin , rahmouni et al . 2010 , siller , dezwaan et al . 2010 ). however , its slow growth rate and its inability to improve mbp - orf27 solubility indicated that ch184 would not be a useful host for 2 - pyrrolidone production . a two - step 2 - pyrrolidone biosynthetic route from glutamate consists of the e . coli glutamate decarboxylase candidate gadb and mbp - orf27 as the 2 - pyrrolidone synthase . not only would the first enzymatic step in the pathway significantly increase the ph of the growth medium , but the two enzymes have a drastic 4 . 2 ph unit difference in their ph optima as well . in native e . coli , production of gaba from glutamate is utilized as an acid resistance mechanism , and gadb is activated under acidic conditions ( 1999 , capitani g 2003 ). gadb has a ph optimum of 3 . 8 and its catalytic activity significantly decreases when ph goes above 5 . 0 ( pennacchietti , lammens et al . 2009 ). previous e . coli gaba overproduction studies using gaba biosynthesis from glutamate as a substrate were performed at ph = 3 . 5 ( le vo , kim et al . 2012 , park , kim et al . 2013 ). the huge ph profile gap of the two enzymatic steps can be challenging for a one pot reaction . indeed , initial inducible 2 - pyrrolidone production trials at ph = 3 . 5 with an integrated pathway ( e . coli strain jz - 337 ) resulted in e . coli cell lysis and no 2 - pyrrolidone production . gadb histidine 465 plays an important role at phs near neutral ; gadb mutants , such as h465a or aht , enable gadb to maintain its relatively high activity at more alkaline ph ( pennacchietti , lammens et al . 2009 ). as shown in the extracellular ph profile for 2 - pyrrolidone biosynthesis strains carrying wild type and mutant gadb ( jz - 336 , jz - 338 ), ph ˜ 5 . 5 resulted in the highest 2 - pyrrolidone titer for both gadb variants ( fig5 a ). δ1 - 14 mutants , which contain a deletion of n terminal peptide responsible for recruiting the gadb hexamer to the membrane , performed slightly worse than their counter parts ( capitani g 2003 ). sensitivity analysis showed that strains carrying gadb aht robustly gave optimum yield within the ph range of 5 . 0 - 5 . 5 , and declined when the exogenous ph drifted above 6 . 0 or below 4 . 5 ( fig5 b ). a gabt knockout , which eliminates gaba transaminase , had little impact on the titer . to increase the flux through the pathway , various inducible promoters were tested ( jz - 336 and jz - 338 , jz - 342 to 344 , jz - 348 to 350 ). production of 2 - pyrrolidone was highest when strong promoters were used , so the t7 promoter was chosen to drive expression of gadb δht and mbp - orf27 for optimal 2 - pyrrolidone production ( fig5 c ). the full set of takara chaperones were tested ( jz - 371 to 380 ) to determine if chaperone proteins would increase the titer by alleviating mbp - orf27 aggregation , and in all cases , the titer decreased between 20 % to 85 % ( fig6 ) ( kazuyo nishihara 1998 , kazuyo nishihara 2000 ). studier &# 39 ; s zym - 5052 is a high density culture medium that enables autoinduction of protein expression in e . coli ( studier 2005 ). strain jz - 344 , grown overnight lb medium , was tested through 2 - stage autoinducible 2 - pyrrolidone production from glutamate : stage i , cell growth to o . d . ˜ 0 . 6 at 37 ° c . ; stage ii , ph titration to 5 . 25 and incubation at 25 ° c . for 2 - pyrrolidone production . fermentation in medium with 9 g / l and 0 g / l of glutamate showed that over 98 % of 2 - pyrrolidone was converted from glutamate supplied in the medium ( fig1 ). overall , 1 . 1 g / l of 2 - pyrrolidone was produced by converting 7 . 7 g / l of l - glutamate within 31 h , achieving 25 % conversion ( fig7 a ). the formation of gaba intermediates occurred rapidly during the first 30 h . during this time , the ph increased from 5 . 25 to 8 . 07 and occurred mainly during the glutamate to gaba conversion period ( fig1 ). there was an approximate 6 - hour time delay between extracellular gaba accumulation and 2 - pyrrolidone formation . gaba was rapidly transformed into 2 - pyrrolidone when mbp - orf27 was solubly expressed in day 1 . 2 - pyrrolidone formation slowed down when mbp - orf27 started to aggregate at around 48 h time point ( fig7 b ). the od of culture decrease from 12 to 8 , presumably due to cell lysis as a result of the high ph . plp is a cofactor for glutamate decarboxylase activity ( capitani g 2003 , pennacchietti , lammens et al . 2009 , takahashi , shirakawa et al . 2012 ); 1 mm , 0 . 1 mm and 0 mm of plp supplementation were compared . plp supplementation had little impact on 2 - pyrrolidone yield ; either the first decarboxylation step was not limited by cofactor or glutamate decarboxylation was not the rate limiting step . e . coli caic was also tested ( bernal , arense et al . 2008 ). although caic has better thermostability and outperformed orf27 in 2 - pyrrolidone biosynthesis during gaba feeding , expression of e . coli caic conferred more growth stress under glutamate feeding conditions , and resulted in only 18 . 7 % of the 2 - pyrrolidone titer of that achieved when engineered orf27 fused with mbp was used . through years of engineering , the petrochemical industry created over 4 , 000 bulk chemicals ( from icis ). this dwarfs the chemicals produced via fermentation . currently , around 200 products are made via microbial fermentation ( excluding food and beverages ), including amino acids , bioactive compounds , etc . however , with advances in synthetic biology , it is now possible to engineer microbes to produce chemicals traditionally made via petrochemical processes at lower cost . due to the differences in raw material and chemistry , the petrochemical processes may be advantaged for relatively simple , symmetric and reduced compounds , yet they cannot match microbial processes when it comes to highly functionalized compounds with higher oxidation states . an important step towards building molecules of increasing complexity is to discover enzymes for substrate activation , such as c — c bond and c — n bond formation ( dougherty and arnold 2009 ). although directed evolution has been applied to modify enzymes for novel substrate recognition , these methods usually require high throughput assays , and the engineered enzyme often suffers from relatively low efficiency and turnover number ( dougherty and arnold 2009 , dietrich , mckee et al . 2010 , esvelt , carlson et al . 2011 ). unfortunately , bioinformatics prediction of an enzyme &# 39 ; s substrate is still in its infancy . here we successfully demonstrated an efficient methodology to scout for enzymes from the secondary metabolite linearmycin a biosynthetic gene cluster that activate y - aminobutyrate . the huge diversity of secondary metabolites and the functional groups embedded in these molecules made them a rich source of gene candidates ( yadav , gokhale et al . 2009 , medema , breitling et al . 2011 ). polyketide biosynthesis follows the colinearity rule and is highly predictable in terms of its biosynthetic pathway , making them useful source of substrate activating enzymes . the discovery of s . aizunensis orf27 enables the first demonstration of 2 - pyrrolidone production from γ - aminobutyrate at mild fermentation conditions . although the dehydration of γ - aminobutyrate to form 2 - pyrrolidone is thermodynamically favorable , due to its high activation barrier , a significant temperature (& gt ; 200 ° c .) is required for the reaction to proceed even in the presence of al 2 o 3 catalyst . this reaction requires several days to complete , and the harsh conditions also lead to off pathway reactions , producing oligomers or cyclic gaba dimer or trimer ( stavila and loos 2013 ). the enzyme orf27 utilize atp to activate the γ - aminobutyrate , presumably generating the labile γ - aminobutyryl ester intermediate , which rapidly cyclizes to form 2 - pyrrolidone . s . aizunensis orf27 belongs to the amp - activating enzyme family . this didomain enzyme was prone to aggregation when heterologously overexpressed in e . coli . previously , sumo , but not mbp fusion , has been used in metabolic engineering to improve enzyme solubility and increase product titers ( shiue and prather 2014 ). in our case , utilizing the mbp fusion alleviated orf27 aggregation and maintained enzymatic activity for prolonged 2 - pyrrolidone production , thus leading to an increased final titer . since functional heterologous expression of enzymes such as p450s , terpene synthases , and polyketide synthases in microbial hosts is a major bottleneck , fusion with solubilizing domains can be a general metabolic engineering strategy . the surprising 2 - pyrrolidone synthase activity of native caic in e . coli highlights the unintended cross talk during metabolic engineering . in the case of caic , previous research concluded that gaba was not caic &# 39 ; s substrate judging from coa product assay ( bernal , arense et al . 2008 ). while substrate profiling for enzymes in the engineered pathway are commonly done to identify “ enzyme hijacking ” by other intracellular metabolites , little attention was paid to the “ enzyme profiling ” of metabolic engineering pathway intermediates , and the impact of “ substrate hijacking of host enzymes ” on microbial growth or product titer . traditional biochemical characterization of enzymes aims to identify native substrates and perform substrate screening under sub - millimolar conditions . activities on substrate analogs or competitive inhibition studies were also performed under similar conditions . however , during metabolic engineering of microbial chemical production , intermediate concentrations can increase to submolar or even molar levels . also , there is a significant difference between the intracellular environment and in vitro assay environment , such as salt concentration , crowdedness , etc . this can lead to unknown and unwanted side reactions , which can reduce yields and create toxic metabolites ( kizer , pitera et al . 2008 ). for instance , caic was reported to have negative activity on gaba in vitro assays . luckily in our case , caic is not essential for e . coli , and caic ko did not impact growth . therefore caic &# 39 ; s competitive inhibition by gaba did not cause a growth defect . however , it is generally necessary to conduct a thorough investigation and check for side activity against intermediates for all enzyme classes of the same family in a native microbial host . as mentioned previously , metabolic engineering is more than simply recruiting various enzymes to the same host ( chaitan khosla 2003 ). enzymes are historically evolved to operate under drastically different conditions ( galperin and koonin 2012 ). in our two enzymatic reactions steps , the ph optimum of e . coli wild type gadb and s . aizunensis orf27 differ by a dramatic 4 . 2 ph units , making it almost impossible to perform a one - pot reaction with the two catalysts . wild type gadb loses 83 % of its activity when the ph reaches 6 . 0 ( s . aizunensis orf27 precipitates ), and almost completely loses its activity at ph = 6 . 5 ( orf27 maintains 20 % of its optimum activity when ph drop to 6 . 5 ). protein engineering shrunk the gap between the ph profiles , and enabled the complete 2 - pyrrolidone biosynthetic pathway from glutamate . in considering overall limitations on the productivity of this system , mbp - orf27 is the rate limiting step , and the enzyme suffers from a tendency to aggregate . it would be desirable to evolve orf27 to be more soluble and stable so that prolonged 2 - pyrrolidone biosynthesis can be sustained during production process . on the other hand , our study opened the door for microbial 2 - pyrrolidone production , transferring the pathway into other glutamate overproduction hosts such as corynebacterium glutamicum are currently under investigation . in this study , we utilized retro - biosynthetic analysis of polyketide natural products as a targeted method to prospect for novel y - aminobutyrate activating enzymes for performing unprecedented reactions such as gaba conversion to 2 - pyrrolidone . e . coli &# 39 ; s native caic , a betaine - coa ligase , was also discovered to be able to catalyze 2 - pyrrolidone formation . protein modification , such as mbp fusion , increased the activity of expressed orf27 . the gadb δht mutant shrunk the ph differences between the two enzymatic steps . metabolic engineering and process optimization collectively improved 2 - pyrrolidone titer from glutamate . 1 . 1 g / l of 2 - pyrrolidone was produced from 9 g / l of glutamate , representing a 25 % conversion . while the present invention has been described with reference to the specific embodiments thereof , it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the true spirit and scope of the invention . in addition , many modifications may be made to adapt a particular situation , material , composition of matter , process , process step or steps , to the objective , spirit and scope of the present invention . all such modifications are intended to be within the scope of the claims appended hereto .