Patent Application: US-86075601-A

Abstract:
this invention relates to pharmaceutical compositions and methods of using non - peptidic cyclophilin - binding compounds in medical conditions involving breakdown of mitochondrial energy metabolism induced by calcium overload , in treating alopecia and promoting hair growth , in treating infections with filarial and helmintic parasites , and in treating and preventing infections with the human immunodeficiency virus .

Description:
one skilled in the art can refer to general reference texts for detailed descriptions of known techniques discussed herein or equivalent techniques . these texts include current protocols in molecular biology ( ausubel , et al ., eds ., john wiley & amp ; sons , n . y ., and supplements through june 1999 ), current protocols in immunology ( coligan , et al ., eds ., john wiley and sons , n . y ., and supplements through june 1999 ), and current protocols in pharmacology ( enna et al ., eds ., john wiley & amp ; sons , n . y ., and supplements through june 1999 ) for example , each of which are specifically incorporated by reference in their entirety . these texts can also be referred to in making or using an aspect of the invention . as noted above , cyclosporin a was the first compound identified to bind a cyp . based on the cyclic structure of cyclosporin a , a number of large , usually cyclic peptides were developed as immunosuppressive compounds that bind cyp . now , unexpectedly , the inventors have found a non - peptidic class of cyp binding compounds with activity in neuronal cells . the following compounds are representative of those useful in the methods of this invention . each of compounds 1 - 4 , 6 , 7 , and 10 - 31 significantly inhibit cyclophilin rotamase activity at a concentration of 10 μm or below , and many inhibit 50 % of cyclophilin rotamase activity at a concentration lower than 5 μm ( ic 50 ), some lower than 1 μm . compounds 6 and 7 possess neurotrophic or neuroprotectant activity . these data demonstrate the broad range of possibilities for a number of structural elements in the compounds of the invention . indeed , a number of substituents are well tolerated . accordingly , the scope of the invention is not limited to those compounds specifically described by formulae i and ii and those depicted in this specification . by performing any one or more of the assays for detecting cyp binding , one skilled in the art can determine whether or not modifications to the r1 - 5 groups , x or y groups , or the value of n for formulae i and ii , result in a cyp binding compound of this invention . the compounds useful in the methods of this invention can be prepared by a number of synthetic routes . the examples below detail schemes 1 to 4 and the preparation of specific compounds . however , one skilled in the art can modify the steps , reactants , and reaction conditions in the examples and schemes to arrive at numerous examples of compounds of the invention . in addition , if particular stereoisomers or mixtures are desired , the starting materials and / or reactants in the preparatory scheme can be selected and used accordingly . alternatively or in addition , particular intermediates can be purified or enriched by chromatographic or enzymatic methods , or by manipulating reaction conditions or selective crystallization , to generate particular final products or mixtures . one skilled in the art is familiar with numerous methods to selectively produce or enrich for desired stereoisomers or mixtures . all of the compounds of the examples , including the intermediates , are specifically included in the compounds of the invention and can be used in the methods of the invention . the compounds of the invention may be prepared as a salt or derivative . various salts and derivatives are known in the art and a non - limiting list of possible choices includes acid salts : acetate , adipate , alginate , aspartate , benzoate , benzenesulfonate , bisulfate butyrate , citrate , camphorate , camphorsulfonate , cyclopentanepropionate , digluconate , dodecylsulfate , ethanesulfonate , fumarate , glucoheptanoate , glycerophosphate , hemisulfate , heptanoate , hexanoate , hydrochloride , hydrobromide , hydroiodide , 2 - hydroxyethanesulfonate , lactate , maleate , methanesulfonate , 2 - naphthalenesulfonate , nicotinate , oxalate , mesylate , dimesylate , pamoate , pectinate , persulfate , 3 - phenylpropionate , phosphates , picrate , pivalate , propionate , succinate , sulfates , tartrate , thiocyanate , tosylate , and undecanoate . base salts may include : amine salts , ammonium salts , alkali metal salts such as sodium and potassium salts , alkaline earth metal salts such as calcium and magnesium salts , salts with organic bases such as dicyclohexylamine salts , n - methyl - d - glucosamine , and salts with amino acids , for example arginine or lysine . nitrogen - containing groups of the compound can be quaternized with agents as : alkyl halides , for example methyl , ethyl , propyl , and butyl chlorides , bromides , or iodides ; dialkyl sulfates , for example dimethyl , diethyl , dibutyl and diamyl sulfates , long chain halides , for example decyl , dodecly , lauryl , myristyl , or stearyl chlorides , bromides , or iodides ; and aralkyl halides , for example benzyl and phenethyl bromides , chlorides , or iodides . the skilled artisan is familiar with methods for producing and testing any suitable salt or derivative . ( see , for example , remington &# 39 ; s pharmaceutical sciences , mack publishing co ., easton , pa ., 18 th edition , specifically incorporated herein by reference .) in general , activity in the nervous system for a particular compound can be identified by assaying for the ability to promote neurite outgrowth , protect neurons from damage by chemical treatments , promote the growth of neurons or neuronal cells , recover lost or damaged motor , functional or cognitive ability associated with nervous tissue or organs of the nervous system , or regenerate neurons . these activities can be useful in treating , diagnosing , or prognosing a number of human disease conditions , including , but not limited to , parkinson &# 39 ; s disease , alzheimer &# 39 ; s disease , amyotrophic lateral sclerosis ( als ), traumatic injury , spinal cord injury , multiple sclerosis , diabetic neuropathy , neuropathy associated with medical treatments such as chemotherapy , ischemia or ischemia - induced injury , stroke , oxygen deprivation , retinopathies , peripheral neuropathies , and neuropathies associated with viral infection . a number of animal model assays and cell culture assays have been developed and can be relied on for their clinical relevance to disease treatments , including the human diseases noted above . each of the following references can be used as a source for these assays , and all of them are specifically incorporated herein by reference in their entirety for that purpose : steiner , et al ., proc . natl . acad . sci . u . s . a . 94 : 2019 - 2024 ( 1997 ); hamilton , et al ., bioorgan . med . chem . lett . 7 : 1785 - 1790 ( 1997 ); mcmahon , et al ., curr . opin . neurobiol . 5 : 616 - 624 ( 1995 ); gash , et al ., nature 380 : 252 - 255 ( 1996 ); gerlach , et al ., eur . j . pharmacol .— mol . pharmacol . 208 : 273 - 286 ( 1991 ); apfel , et al ., brain res . 634 : 7 - 12 ( 1994 ); wang , et al ., j . pharmacol . exp . therap . 282 : 1084 - 1093 ( 1997 ); gold , et al ., exp . neurol . 147 : 269 - 278 ( 1997 ); hoffer et al ., j . neural transm . [ suppl .] 49 : 1 - 10 ( 1997 ); and lyons , et al ., pnas 91 : 3191 - 3195 ( 1994 ). preferred methods for detecting neuronal activity include a neuroprotective assay , in which a compound is tested for the ability to protect against treatment causing glutamate neurotoxicity . sensory neuronal cultures ( drg ) can also be assayed for neurite outgrowth , an assay for neurotrophic activity . cultured cells are treated with a compound of the invention and later assayed for the presence of new neurite fibers . immunohistochemistry can aid in the visualization and quantitation of neurites as compared to control . the compounds of the invention can also be used to promote the establishment or maintenance of tissue or cell cultures . similar to the use for promoting neuronal cell growth , the compounds can be added to primary , transformed , or established cell cultures . particularly in the case of neuronal cells , the compounds can induce growth in culture and extend the culture lifetime of cells . in addition to or in the alternative to the activity in neuronal or nervous system cells and the medical disorders disclosed throughout this specification , the compounds of the invention bind cyp . a recognized method for assessing the affinity of the compound to cyclophilin is the rotamase inhibition assay . for this purpose , the following references are specifically incorporated by reference and can be relied on to make assays of rotamase inhibition : fischer , et al ., biomed . biochem . acta 43 : 1101 - 1112 ( 1984 ); kofron , et al ., biochem . 30 : 6127 - 6134 ( 1991 ); kofron et al ., j . am . chem . soc . 114 : 2670 - 2675 ( 1992 ); harrison et al ., biochem . 29 : 3813 - 3816 ( 1990 ); lang et al ., nature 329 : 268 - 270 ( 1987 ); mucke et al ., biochem . 31 : 7848 - 7854 ( 1992 ); schonbrunner et al ., j . biol . chem . 266 : 3630 - 3635 ( 1991 ); hsu et al ., j . am . chem . soc . 112 : 6745 - 6747 ( 1990 ); and justice et al ., biochem . biophys . res . commun . 171 : 445 - 450 ( 1990 ). the compounds of the invention have utility in pharmacological compositions for the treatment and prevention of various medical conditions or for various in vitro and cell culture treatments . the compounds may have utility in pharmacological compositions for the treatment and prevention of hiv - infection , promotion of hair growth , immunosuppression , mitochondrial disorders , treatment of parasitic infections , treatment of ischemic conditions , conditions associated with optic nerve damage , or traumatic injury to nervous tissue — such as , for example , nerve damage incident to prostate surgery / prostatectomy , which results in erectile dysfunction . the compounds of the invention may be prepared as a salt or derivative , as described above . a compound of the invention can be administered to an animal or human patient by itself or in pharmaceutical compositions where it is mixed with suitable carriers or excipients , at doses to treat or ameliorate various conditions . the compounds according to the present invention preferably have sufficient stability , potency , selectivity , solubility and availability to be safe and effective in treating diseases , injuries and other abnormal conditions or insults to the central nervous system , the peripheral nerves , and other organs . a therapeutically effective dose refers to that amount of the compound sufficient to effect an activity in a nerve or neuronal cell , to produce a detectable change in a cell or organism , or to treat a disorder in a human or other mammal . the word “ treat ” in its various grammatical forms as used in relation to the present invention refers to preventing , curing , reversing , attenuating , alleviating , minimizing , suppressing , ameliorating or halting the deleterious effects of a disease state , disease progression , injury , wound , ischemia , disease causative agent ( e . g ., bacteria , protozoans , parasites , fungi , viruses , viroids and / or prions ), surgical procedure or other abnormal or detrimental condition ( all of which are collectively referred to as “ disorders ,” as will be appreciated by the person of skill in the art ). a “ therapeutically effective amount ” of a compound according to the invention is an amount that can achieve effective treatment , and such amounts can be determined in accordance with the present teachings by one skilled in the art . the methods of the present invention comprise ( i .) administration of a compound of formula i or ii , where the compound is itself therapeutically active in the treatment of the targeted medical condition , or ( ii .) administration of a prodrug of a compound of formula i or ii , wherein such prodrug is any compound which is capable of undergoing metabolic conversion to a compound of formula i or ii following administration , or ( iii .) administration of a compound of formula i or ii where the compound is capable of undergoing metabolic conversion to a metabolite following administration , and where the metabolite is therapeutically active in the treatment of the targeted medical condition , or ( iv .) administration of a metabolite of a compound of formula i or ii , where the metabolite is therapeutically active in the treatment of the targeted medical condition . thus , the use of a compound of formula i or ii in the methods of the present invention explicitly includes not only the use of the compound itself , but also the modifications ii , iii , and iv discussed in this paragraph , and all such modifications are explicitly intended to be within the scope of the following claims . therapeutically effective doses may be administered alone or as adjunctive therapy in combination with other treatments for the medical conditions within the scope of the methods of this invention , or associated diseases . techniques for the formulation and administration of the compounds of the instant application are known in the art , and may , for example , be found in remington &# 39 ; s pharmaceutical sciences , mack publishing co ., easton , pa ., 18 th edition ( 1990 ). suitable routes of administration may , for example , include oral , rectal , transmucosal , buccal , or intestinal administration ; parenteral delivery , including intramuscular , subcutaneous , intramedullary injections , as well as intrathecal , direct intraventricular , intravenous , intraperitoneal , intranasal , or intraocular injections , and optionally in a depot or sustained release formulation . furthermore , one may administer the agent of the present invention in a targeted drug delivery system , for example in a liposome coated with an antibody . the liposomes will be targeted to and taken up selectively by cells expressing the appropriate antigen . the pharmaceutical compositions of the present invention may be manufactured in a manner that is itself known , e . g ., by means of conventional mixing , dissolving , emulsifying , encapsulating , entrapping , or lyophilizing processes . pharmaceutical compositions for use in accordance with the present invention thus may be formulated in conventional manner using one or more physiologically acceptable carriers comprising excipients and auxiliaries , which facilitate processing of the active compounds into preparations , which can thus be used pharmaceutically . for injection , the agents of the invention may be formulated in aqueous solutions , preferably in physiologically compatible buffers , such as hank &# 39 ; s solution , ringer &# 39 ; s solution , or physiological saline buffer . for transmucosal or buccal administration , penetrants appropriate to the barrier to be permeated may be used in the formulation . such penetrants are known in the art . for oral administration , the compounds can be formulated readily by combining the active compounds with pharmaceutically acceptable carriers , well known to those in the art . such carriers enable the compounds of the invention to be formulated as tablets , pills , capsules , liquids , quick - dissolving preparations , gels , syrups , slurries , suspensions and the like , for oral ingestion by a patient to be treated . pharmaceutical preparations for oral use can be obtained as a solid excipient , optionally grinding a resulting mixture , and processing the mixture of granules , after adding suitable auxiliaries , if desired , to obtain tablets . suitable excipients include , in particular , fillers such as sugars , including lactose , sucrose , mannitol , or sorbitol ; cellulose preparations such as , for example , maize starch , wheat starch , rice starch , potato starch , gelatin , gum tragacanth , methyl cellulose , hydroxypropylmethyl - cellulose , sodium carboxymethylcellulose , and / or polyvinylpyrrolidone ( pvp ). the pharmaceutical compositions also may comprise suitable solid or gel phase carriers or excipients . examples of such carriers or excipients include calcium carbonate , calcium phosphate , various sugars , starches , cellulose derivatives , gelatin , and polymers such as polyethylene glycols . if desired , disintegrating agents may be added , such as the cross - linked polyvinyl pyrrolidone , agar , or alginic acid or a salt thereof such as sodium alginate or a number of others disintegrants ( see , for example , remington &# 39 ; s pharmaceutical sciences , mack publishing co ., easton , pa ., 18 th edition ( 1990 )). for administration by inhalation , the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebulizer , with the use of a suitable propellant , e . g ., dichlorodifluoromethane , trichlorofluoromethane , dichlorotetrafluoroethane , carbon dioxide , pressurized air , or other suitable gas or mixture . in the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount . capsules and cartridges of e . g . gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch . the compounds may be formulated for parenteral administration by injection , e . g ., by bolus injection or continuous infusion . pharmaceutical formulations for parenteral administration include aqueous solutions of the active compounds in water - soluble form . additionally , suspensions or emulsions of the active compounds may be prepared as appropriate oily injection preparations . suitable lipophilic solvents or vehicles include fatty oils such as sesame oil , or synthetic fatty acid esters , such as ethyl oleate or triglycerides , or liposomes . aqueous injection suspensions may contain substances , which increase the viscosity of the suspension , such as sodium carboxymethyl cellulose , sorbitol , or dextran . optionally , suspensions may also contain suitable stabilizers , or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions . alternatively , the active ingredient may be in powder form for constitution with a suitable vehicle , e . g ., sterile pyrogen - free water , before use . the compounds may also be formulated in rectal compositions such as suppositories , e . g ., containing conventional suppository bases such as cocoa butter or other glycerides . in addition to the formulations described previously , the compounds may also be formulated as a depot preparation . such long acting formulations may be administered by implantation ( for example subcutaneously or intramuscularly ) or by intramuscular injection . thus , for example , the compounds may be formulated with suitable polymeric or hydrophobic materials ( for example as an emulsion in an acceptable oil ) or ion exchange resins , or as sparingly soluble derivatives , for example , as a sparingly soluble salt . the compounds of the invention may further be formulated in pharmaceutical or cosmetic compositions for topical application to the skin in the form of an aqueous , alcoholic , aqueous / alcoholic or oily solution , or of a dispersion of the lotion or serum type , of an emulsion having a liquid or semi - liquid consistency of the milk type , obtained by dispersion of a fatty phase in an aqueous phase ( o / w ) or vice versa ( w / o ), or of a suspension or of an emulsion with a soft consistency of the aqueous or anhydrous gel , foam or cream type , or , alternatively , of microcapsules or microparticles , or of a vesicular dispersion of ionic and / or nonionic type , or may further be administered in the form of an aerosol composition comprising a pressurized propellent agent . pharmaceutical compositions are distinguished from cosmetic compositions by the concentration or strength of the active ingredient , and the intended use of the composition , as will be appreciated by one skilled in the art . the compounds of the invention can also be formulated into various compositions for hair care and , in particular , shampoos , hair - setting lotions , treating lotions , styling creams or gels , dye compositions ( in particular oxidation dyes ), optionally in the form of color - enhancing shampoos , hair - restructuring lotions , permanent - wave compositions , and the like . pharmaceutical or cosmetic compositions comprising compounds of the invention can also contain additives and adjuvants which are conventional in the cosmetics field , such as gelling agents , preservatives , antioxidants , solvents , fragrances , fillers , screening agents , odor absorbers and colorants . the amounts of these different additives and adjuvants are those typically employed in the cosmetics field and range , for example , from 0 . 01 % to 20 % of the total weight of the composition , preferably 0 . 1 % to 10 %, and more preferably 0 . 5 % to 5 %. in addition to one or several compounds of the invention , compositions for topical application may further contain additional agents already known in the art to promote hair growth or to prevent or retard hair loss , such as , without limitation , tocopherol nicotinate , benzyl nicotinate or 2 , 4 - diamino - 6 - piperidinopyrimidine 3 - oxide , or may contain other active agents such as antibacterial agents , antiparasitic agents , antifungal agents , antiviral agents , anti - inflammatory agents , antipruriginous agents , anaesthetic agents , keratolytic agents , antiseborrhoeic agents , antidandruff agents , or antiacne agents . the cosmetic or pharmaceutical compositions according to the invention can be topically applied onto the alopecic areas of the scalp and skin of an individual and optionally maintained in contact for a number of hours and optionally rinsed . it is possible , for example , to apply the composition containing an effective amount of at least one compound of the invention in the evening , to retain the composition in contact overnight and optionally to shampoo in the morning . these applications can be repeated daily for one or a number of months , depending on the particular individuals involved . liposomes and emulsions are well known examples of delivery vehicles or carriers for hydrophobic drugs . certain organic solvents such as dimethylsulfoxide also may be employed , although usually at the cost of greater toxicity . additionally , the compounds may be delivered using a sustained - release system , such as semipermeable matrices of solid hydrophobic polymers containing the therapeutic agent . various suitable sustained - release materials have been established and are well known by those skilled in the art . sustained - release capsules may , depending on their chemical nature , release the compounds for a few days up to over 100 days , preferably 7 - 28 days . depending on the chemical nature and the biological stability of the therapeutic reagent , additional strategies for stabilization may be employed . pharmaceutical compositions suitable for use in the present invention include compositions wherein the active ingredients are contained in an effective amount to achieve their intended purpose , to effect a therapeutic benefit , or to effect a detectable change in the function of a cell , tissue , or organ . more specifically , a therapeutically effective amount means an amount effective to prevent the development of or to alleviate the existing symptoms of the subject being treated . determining the effective amount is well within the capability of those skilled in the art , especially in light of the detailed disclosure provided herein . toxicity and therapeutic efficacy of the compounds or compositions can be determined by standard pharmaceutical , pharmacological , and toxicological procedures in cell cultures or experimental animals . for example , numerous methods for determining the ld 50 ( the dose lethal to 50 % of the population ) and the ed 50 ( the dose therapeutically effective in 50 % of the population ) exist . the dose ratio between toxic and therapeutic effects is the therapeutic index , which can be expressed as the ratio between ld 50 and ed 50 . compounds and compositions exhibiting high therapeutic indices are preferred . the data obtained from cell culture assays or animal studies can be used in formulating a range of dosages for use in humans . ( see , for example , fingl et al ., in the pharmacological basis of therapeutics , ch . 1 p . 1 ( 1975 ).) a subset of the compounds of formula i may be prepared by reacting isocyanates with amines , as depicted in scheme 1 below . one skilled in the art is familiar with suitable reaction conditions and parameters . the synthesis of compound 9 , detailed below , illustrates . a mixture of phenyl - 1 , 3 - diisocyanate ( 0 . 1 mmol ), cyclohexylamine ( 0 . 25 mmol ), and diisopropylethylamine ( 0 . 1 mmol ) in 1 ml dichloromethane was stirred overnight . the resulting precipitate was washed with water and ether to provide ( cyclohexylamino )- n -{ 3 -[( cyclohexylamino ) carbonylamino ] phenyl } formamide ( gpi 7104 ) as a white solid , having [ 0106 ] 1 h nmr ( cdcl 3 , 400 mhz ) peaks as follows : δ 0 . 88 ( m , 6h ); 1 . 07 ( m , 4h ); 1 . 28 ( m , 2h ); 1 . 41 ( m , 4h ); 1 . 59 ( m , 4h ); 6 . 73 ( m , 3h ); 7 . 17 ( s , 1h ); 7 . 52 ( m , 3h ); 7 . 78 ( m , 1h ). another subset of compounds of formula i may be prepared by the route depicted in scheme 2 below . one skilled in the art is familiar with suitable reaction conditions and parameters . the synthesis of compound 14 , detailed below , illustrates . a solution of 1 , 3 - bis - benzoyl chloride ( 0 . 99 g , 4 . 9 mmol ), 3 , 5 - dichloroaniline ( 1 . 58 g , 9 . 75 mmol ), and triethylamine ( 2 ml , 14 . 3 mmol ) in 50 ml of dichloromethane was stirred at room temperature overnight . the reaction mixture was washed with water and the resulting precipitated solid was collected by filtration to deliver 1 . 94 g of crude solid . recrystallization from acetone furnished analytically pure material with a mp = 260 - 262 ° c . and 1 h nmr ( dmso , 400 mhz ) peaks at : δ 7 . 37 ( m , 2h ); 7 . 76 ( t , 1h , j = 7 . 8 ); 7 . 93 ( d , 4h , j = 1 . 8 ); 8 . 18 ( dd , 2h , j = 1 . 7 , 7 . 8 ); 8 . 52 ( d , 1h , j = 1 . 5 ); 10 . 73 ( s , 2h ). the theoretical atomic composition for c 20 h 12 n 2 o 2 cl 4 [ c , 52 . 90 ; h , 2 . 66 ; n , 6 . 17 ; cl , 31 . 23 ], compares favorably with that found experimentally [ c , 53 . 04 ; h , 2 . 72 ; n , 6 . 11 ; cl , 31 . 35 ]. a subset of the compounds of the invention with unsymmetrical substituents off of the cyclohexyl or phenyl ring structure of formulae i or ii may be prepared by scheme 3 , below . one skilled in the art is familiar with suitable reaction conditions and parameters . the synthesis of compounds 13 and 15 , detailed below , illustrates . a stirred solution of 3 - nitrophenol ( 1 . 39 g , 10 mmol ), 1 - naphthaleneethanol ( 1 . 89 g , 11 mmol ), and triphenylphosphine ( 2 . 9 g , 11 mmol ) in 100 ml of tetrahydrofuran was treated with a solution of 2 . 22 g ( 11 mmol ) of diisopropylazodicarboxylate added dropwise . the resulting mixture was stirred overnight , and then concentrated and redissolved in a minimum amount of ethyl acetate . purification on a silica gel column , eluting with 10 % ethyl acetate in hexane , delivered 2 . 0 g of the ether . to a refluxing suspension of 150 mg “ wet ” raney - nickel in 100 ml of ethanol containing 1 . 70 g ( 34 mmol ) of hydrazine hydrate was added the nitro compound . after refluxing for an additional 15 minutes , the mixture was cooled and filtered through celite to remove solids . removal of the solvent furnished the product as an orange oil , which crystallized on standing and was used without further purification for the next step . a solution of 3 -( 2 - phenylethoxy ) phenylamine ( 200 mg , 0 . 76 mmol ), 1 - naphthoyl chloride ( 160 mg ; 0 . 84 mmol ), and triethylamine ( 0 . 2 ml , 1 . 43 mmol ) in 50 ml of dimethylacetamide was stirred overnight . the solvent was removed and the residue dissolved in ethyl acetate and washed with water and brine . after concentration , a clear oil was obtained that crystallized on standing . this was purified on a silica gel column , eluting with methylene chloride , to obtain 200 mg of compound # 15 as a white solid , mp = 191 - 193 ° c ., and 1 h nmr ( dmso , 400 mhz ) peaks of : δ 3 . 56 ( t , 2h , j = 6 . 8 ); 4 . 31 ( t , 2h , j = 6 . 9 ); 6 . 71 ( dd , 1h , j = 2 . 1 , 8 . 1 ); 7 . 25 ( t , 1h , j = 8 . 0 ); 7 . 34 ( bd , 1h , j = 8 . 4 ); 7 . 47 - 8 . 22 ( m , 15h ); 10 . 52 ( s , 1h ). the theoretical atomic composition for c 29 h 23 no 2 [ c , 83 . 43 ; h , 5 . 55 ; n , 3 . 35 ] compares favorably to that found experimentally [ c , 83 . 29 ; h , 5 . 69 ; n , 3 . 39 ]. a solution of 3 -( 2 - phenylethoxy ) phenylamine ( 200 mg , 0 . 76 mmol ), 1 - naphthylsulfonyl chloride ( 190 mg , 0 . 84 mmol ), and triethylamine ( 0 . 2 ml , 1 . 43 mmol ) was stirred overnight and worked up as described in the previous example . purification of the crude product delivered 210 mg of compound 13 , mp = 165 - 167 ° c ., and 1 h nmr ( dmso , 400 mhz ) peaks of : δ 3 . 42 ( t , 2h , j = 6 . 8 ); 4 . 10 ( t , 2h , j = 6 . 9 ); 6 . 48 - 6 . 60 ( m , 3h ); 7 . 01 ( t , 1h , j = 8 . 1 ); 7 . 40 - 8 . 20 ( m , 13h ); 8 . 70 ( d , 1h , j = 8 . 6 ); 10 . 68 ( s , 1h ). the theoretical atomic composition for c 28 h 23 nso 3 [ c , 74 . 15 ; h , 5 . 11 ; n , 3 . 09 ; s , 7 . 07 ] compares favorably with that found experimentally [ c , 73 . 88 ; h , 5 . 05 ; n , 3 . 06 ; s , 7 . 03 ]. additional examples of compounds of the invention may be prepared as depicted in scheme 4 below . 3 - aminobenzoic acid ( 5 . 0 g , 36 . 5 mmol ) was dissolved in 150 ml of 2n naoh . dioxane ( 100 ml ) was added , followed by 9 . 6 g ( 44 mmol ) of tert - butyl dicarbonate added slowly , with stirring . after the addition was complete , the mixture was stirred overnight . it was diluted with water and washed with ether ( 3 portions ). the aqueous phase was acidified with 20 % citric acid , and the resulting purplish solid was collected by filtration and recrystallized from ethyl acetate to obtain 1 . 6 g of the boc - protected amine . a solution of 3 -[( tert - butoxy ) carbonylamino ] benzoic acid ( 250 mg , 1 . 05 mmol ), 1 - naphthylmethylamine ( 170 mg , 1 . 05 mmol ), diethyl cyanophosphonate ( 260 mg , 1 . 6 mmol ), and triethylamine ( 0 . 22 ml , 1 . 6 mmol ) in acetonitrile was stirred overnight . the solvent was evaporated , and the residue was partitioned between ethyl acetate and 1n hcl . the layers were separated , and the organic phase was washed twice more with 1n hcl , then 3 times each with water and brine . the solvent was removed in vacuo , and the crude product was purified on a silica gel column , eluting with 20 % ethyl acetate in hexane , to deliver 270 mg of the amide . { 3 -[( tert - butoxy ) carbonylamino ] phenyl }- n -( naphthylmethyl ) formamide ( 270 mg , 0 . 72 mmol ) was dissolved in 25 ml of dichloromethane and treated with 7 ml of 2n hcl in ether . after stirring overnight , the precipitate was collected by filtration and dried under vacuum . the aniline ( 190 mg , 0 . 61 mmol ) was dissolved in dimethylacetamide ( 10 ml ), and 3 , 5 - dichlorobenzoyl chloride ( 130 mg , 0 . 61 mmol ) and triethylamine ( 0 . 5 ml ) were added and the resulting mixture was stirred overnight . the product was worked up as described above and recrystallized from ethyl acetate to provide compound 16 as a white crystalline solid , mp = 205 - 208 ° c ., and 1 h nmr ( dmso , 400 mhz ) peaks of : δ 4 . 97 ( d , 2h , j = 5 . 76 ); 7 . 45 - 8 . 26 ( m , 14h ); 9 . 10 ( t , 1h , j = 5 . 76 ); 10 . 57 ( s , 1h ). the theoretical atomic composition for c 25 h 18 n 2 o 2 cl 2 [ c , 66 . 83 ; h , 4 . 04 ; n , 6 . 23 ; cl , 15 . 78 ] compares favorably with that found experimentally [ c , 66 . 73 ; h , 4 . 15 ; n , 6 . 16 ; cl , 15 . 81 ]. a number of substrates for rotamase are known in the art or can be derived from those known . typically , the substrate contacts a sample containing a protein with rotamase activity and the conversion of the substrate is detected after a period of time . the method for detecting conversion of the substrate will vary with the particular substrate chosen . one method has been termed the k i test ( see harding , et al ., nature , 341 : 758 - 760 ( 1989 ). the cis - trans isomerization of an alanine - proline bond in a model substrate , n - succinyl - ala - ala - pro - phe - p - nitroanilide , is monitored spectrophotometrically in a chymotrypsin - coupled assay . the action of chymotrypsin releases p - nitroaniline from only the trans form of the substrate . the amount of p - nitroaniline can be monitored in a spectrophotometer , for example . other methods of detecting the presence of p - nitroaniline can also be used . the inhibition of this reaction caused by different concentrations of inhibitor is determined and the data is analyzed as a change in first - order rate constant as a function of inhibitor concentration , which yield the k i value . the following were added to a plastic cuvette : 950 ml of ice cold assay buffer ( 25 mm hepes , ph 7 . 8 , 100 mm nacl ), 10 μl of cyp a ( 2 . 5 μm in 10 mm tris - cl ph 7 . 5 , 100 mm nacl , 1 mm dithiothreitol ), 25 μl of chymotrypsin ( 50 mg / ml in 1 mm hcl ), and 10 μl of test compound , at various concentrations , in dimethyl sulfoxide . the reaction was initiated by the addition of 5 μl of substrate ( succinyl - ala - phe - pro - phe - para - nitroanilide , 5 mg / ml in 470 mm licl in trifluoroethanol ). the absorbance at 390 nm versus time was monitored for 90 seconds using a spectrophotometer . the inhibition values obtained for representative compounds are listed in the following table i , and refer to the percent of rotamase activity that is inhibited by the compound when the compound is present at a concentration of 10 μm . the higher the percentage , the more the compound inhibits rotamase , which in turn means the more active the compound is at binding or interacting with cyp . the ic 50 values refer to the concentration that inhibits 50 % of the rotamase activity in a sample . the lower the value , the more active the compound is at binding or interacting with cyp . while cyp a is used in these examples , other cyp proteins can be substituted . similar methods can be used with other immunophilins , such as the fkbps , to demonstrate the presence or absence of fkbp binding activity . preferred compounds have an ic 50 ≦ 1 μm for inhibition cyclophilin rotamase activity , more preferably ≦ 500 nm , and most preferably ≦ 100 nm . especially preferred compounds , because of their selectivity , may also have an ic 50 ≧ 10 μm , or ≧ 50 μm , for inhibition of fkbp rotamase activity . table i % inhibition ic 50 % inhibition ic 50 compd at 10 μm ( μm ) compd at 10 μm ( μm ) 1 100 6 19 98 2 . 9 3 100 0 . 6 20 100 4 . 85 4 100 0 . 80 21 100 1 . 92 6 100 1 . 02 22 100 8 . 2 7 100 1 . 9 23 100 4 . 2 10 99 4 . 5 24 100 5 . 09 11 93 4 . 0 25 100 3 . 25 12 47 . 7 9 . 5 26 100 5 . 64 13 100 0 . 83 27 100 8 . 55 14 100 0 . 97 28 100 2 . 95 15 100 0 . 74 29 89 6 . 2 16 100 1 . 0 30 88 3 . 95 17 99 0 . 65 31 100 3 . 9 18 100 0 . 52 32 n . d . — 33 2 protection of cells from calcium - related physiological stress can be assessed in a neuroprotection assay . organotypic slice cultures of rat spinal cord tissue display a protracted loss of ventral horn motoneurons when exposed to the glutamate uptake inhibitor tha . all cultures were derived from postnatal day 8 ( p8 ) sprague - dawley rat lumbar spinal cord slices of 325 micron thickness . each experiment consisted of two 6 - well plates with 5 slices from 4 different animals per well . media changes were performed every 3 to 4 days . cultures were treated with tha [ l (−)- threo - 3 - hydroxyaspartic acid ; tocris cookson inc ., ballwin , mo .] at 200 μm + compound ( 10 μm ) after one week in culture . the control was an untreated sample with 0 . 1 % dmso as vehicle . the tha control was a tha treated sample with 0 . 1 % dsmo as vehicle . two wells were used per condition . one media change with new tha and compounds was performed . the experiment was stopped 6 to 8 days following drug treatment ( 13 - 15 total days in vitro , div ) as dictated by visual assessment of lesion , by fixation with 4 % paraformaldehyde / 0 . 1 m phosphate buffer for 30 minutes . slices were permeabilized with 100 % cold methanol for 10 minutes . slices were transferred to staining wells . the slices were blocked with 10 % horse serum / tbs . primary antibody incubation was overnight at 4 ° c . with smi - 32 antibody 1 : 5000 in 2 % horse serum / tbs . smi - 32 is specific towards the unphosphorylated h neurofilament subunit , and was here utilized as a marker for ventral horn motoneurons . a vectastain abc elite kit with rat absorbed anti - mouse secondary antibody was used with dab to stain the slices . the slices were mounted under dpx mounting solution for microscopy . quantification of surviving neurons was performed on a zeiss axiovert microscope . neuronal survival was determined by observing an intact neuronal cell body with processes located ventrally of the central canal in each hemisphere . this correlated to laminae vii , viii and ix . each hemisphere was counted individually . the statistics were performed with statview software on a minimum of three different experiments per condition and significance was determined as compared to tha control . the percent of protection was determined from the average number of living neurons by the following equation : ( drug treatment condition — tha control )/( untreated control - tha control ). typical results are shown in fig1 - 6 . inhibition of mitochondrial permeability transition in a spectrophotometric large amplitude mitochondrial swelling assay fresh rat liver mitochondria are prepared from male sprague - dawley rats as described by broekemeier , et al ., j . biol . chem . 260 : 105 - 113 ( 1985 ). incubations are conducted at room temperature in an assay buffer containing 10 mm sodium succinate , 3 mm hepes ( ph 7 . 4 ), 5 μm rotenone , 0 . 5 μg / ml oligomycin , 10 μm cacl 2 , and mannitol / sucrose at a ratio of 3 : 1 to yield an osmotic strength of 300 mosmoles . five μl of the isolated mitochondria preparation and 5 μl of compound or vehicle solution are added at various concentrations and optical density ( od ) is read at 540 nm for one minute to obtain a baseline reading . ten μl of ruthenium red solution is added to yield a final concentration of 1 μm , and od 540 is monitored for an additional minute . twenty - five μl of fluoro - carbonyl cyanide solution is added to yield a final concentration of 4 μm , and od 540 is monitored for an additional 4 - 5 minutes . mitochondrial permeability transition is manifested as a progressive drop in net absorbance as the mitochondria swell . the ability of the compounds of formulae i or ii to inhibit mitochondrial permeability transition and swelling can be expressed as ic 50 values . compounds of formulae i or ii significantly inhibit the progressive drop of net absorbance at od 540 , and inhibit the mitochondrial permeability transition in a dose - dependent manner . in vivo protective effects in an animal model of cerebral stroke male sprague dawley rats , weighing 260 - 290 g , are used in determining the protective effects of the compounds of the invention against ischemia - induced brain damage . the compounds are dissolved in 50 mm hepes buffered saline or another physiologically acceptable vehicle , and the ph is adjusted to 7 . 4 before administration . the compound is administered intravenously 60 min following experimental medial cerebral artery occlusion ( mcao ) at a bolus dose of , e . g ., 100 mg / kg immediately followed by an infusion dose of 20 mg / kg / hr for 4 hours . mcao surgery : the intraluminal filament model of transient mcao is well established in the art [ see , e . g ., lu , et al ., eur . j . pharmacol . 408 : 233 - 239 ( 2000 )]. briefly , under 1 . 5 % halothane anesthesia , the rat common carotid artery is exposed at the level of external and internal carotid artery bifurcation . the external carotid artery ( eca ) and its branches are cauterized and cut . a piece of 3 - 0 monofilament nylon suture with a blunted tip is introduced into the internal carotid artery ( ica ) via the proximal end of the eca stump . the suture is advanced through the carotid canal to the origin of the mca where it blocks the blood flow to its entire territory . at the end of the 2 hour occlusion period , the rat is re - anesthetized and the suture is carefully pulled back to the eca stump to allow reperfusion . during the surgery , the animal &# 39 ; s body temperature is maintained at 37 . 0 ° c . via a heating blanket . the experimental animals are sacrificed following 22 hr of reperfusion . the brains are removed and cut into seven 2 - mm thick coronal slices , stained with 1 % 2 , 3 , 5 - triphenytetrazolium chloride ( ttc ), and subsequently imaged using a computer - assisted digital imaging analysis system . the ischemic injury is quantified based on the volume of the infarct tissue completely lacking ttc staining . the total infarct volume and the infarct volumes of the cortical and subcortical regions of each rat are used for statistical analysis . a one - factor analysis of variance can be used for comparison of treatment effects . the difference between groups is considered statistically significant at p & lt ; 0 . 05 . administration of compounds of formulae i or ii lead to a significant reduction in infarct volume as compared to vehicle - treated animals . in vivo protective effects in an animal model of myocardial infarction the surgical procedure and protocol for inducing experimental myocardial infarction is itself well - established in the art [ see , e . g ., kukreja , et al . , mol . cell . biochem ., 195 : 123 - 131 ( 1999 )]. briefly , male sprague - dawley rats ( 225 - 300 g ) are anaesthetized with 65 mg / kg sodium pentobarbital i . p ;. following tracheotomy , animals are mechanically ventilated using 35 % o 2 / 65 % n 2 at 50 strokes / min . and a stroke volume of 2 ml , and maintained at 37 . 0 ° c . using a heating blanket . electrocardiographic leads are attached to subcutaneous electrodes to monitor either limb leads i , ii or iii . the right carotid artery is cannulated and connected to a pressure transducer to monitor arterial pressure throughout the experiment , and the right jugular vein is cannulated to allow intravenous administration of compounds of the invention . the compounds are dissolved in 50 mm hepes buffered saline or another physiologically acceptable vehicle , and the ph is adjusted to 7 . 4 before administration . the compound is administered intravenously 20 min prior to experimental coronary artery occlusion at a bolus dose of , e . g ., 100 mg / kg , immediately followed by an infusion dose of 20 mg / kg / hr for 140 minutes . a left thoracotomy is performed at the fourth intercostal space and the heart exposed . a 5 - 0 silk suture with a traumatic needle is then passed around the left coronary artery midway between the atrioventricular groove and the apex , and the ends of the suture thread are passed through a piece of vinyl tubing to form a snare . the coronary artery is transiently occluded by tightening and fixing the snare . myocardial ischemia can be confirmed visually by regional cyanosis of the exposed heart , hypokinetic movement of the heart muscle , or by st segment elevation / depression or t wave inversion on the electrocardiogram . the snare is released after 30 minutes and reperfusion is visually confirmed by hyperemia over the previously cyanotic area of the heart muscle , and by hemodynamic improvement in blood pressure . following 90 minutes of reperfusion , the snare is again tightened and approximately 1 ml of evan &# 39 ; s blue dye is injected as a bolus vial the jugular vein catheter . the animals are sacrificed immediately , the hearts are removed , frozen , and cut from apex to base into 6 - 8 transverse 2 mm - thick slabs . the area at risk is determined by the absence of evan &# 39 ; s blue staining . the slices are then incubated in 1 % ttc solution for visualization of viable tissue . the infarct volume and area at risk are quantitated using a commercially available image analysis system . administration of compounds of formulae i or ii leads to a significant dose - dependent reduction in infarct volume as compared to animals treated with vehicle alone . experimental methods useful in assessing the ability of the present compounds to protect from cancer chemotherapy - induced alopecia are themselves established in the art . see , e . g ., maurer , et al . am . j . pathol . 150 ( 4 ): 1433 - 41 ( 1997 ). in addition , a useful experimental model for assessing the ability of compounds to induce hair growth in bald human scalp from subjects with male pattern baldness has been reported . sintov , et al ., int . j . pharm . 194 : 125 - 134 ( 2000 ). simple procedures for the assessment of hair revitalizing properties of experimental compounds have been disclosed previously by the inventors . see , e . g ., u . s . pat . no . 6 , 194 , 440 b1 . these and other publications referenced herein can be relied upon to assess the hair growth - promoting and hair loss - retarding properties of compounds of formulae i or ii . the following procedure illustrates : mice of the c57b1 / 6 strain , aged 7 - 8 weeks , are housed individually . under light ether anaesthesia , an area of about 2 cm by 2 cm of the lower back / hindquarter region is shaved to remove all existing hair . care is taken to avoid scrapes , cuts or abrasions of the skin . a pinkish color of the skin confirms that all animals are in the telogen phase of the hair growth cycle . groups of 10 animals are treated topically with 20 % propylene glycol vehicle , or with compounds of the invention at concentrations ranging from 0 . 1 μm to 100 μm per milliliter vehicle . compounds are topically administered three times per week , and hair growth is assessed weekly by a blinded observer on a scale of 0 ( no growth ) to 5 ( complete hair growth over shaved area ). the compounds of the invention induce the growth of hair in a dose - dependent manner , and significantly shorten the time elapsed until the shaved area is covered by hair , as compared to the shaved area of vehicle - treated animals . as noted above , the specific examples should not be interpreted as a limitation to the scope of the invention . instead , they are merely exemplary embodiments one skilled in the art would understand from the entire disclosure of this invention . each of the references cited below or in the text above can be relied on to make and use any aspect of this invention . while particular uses and references are discussed above , this should not be taken as a limitation on the use of any particular reference . all the references are specifically included into this text by reference , in their entirety . ausubel , et al ., eds ., current protocols in molecular biology , john wiley & amp ; 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