Patent Application: US-83505107-A

Abstract:
the following provisional patent application refers to the methods and compositions relating to a novel use for enzymatic catalysis of c21 - h30 - o2 ) as an insect repellent , bactericide , and fungicide and dispensation methods as commercial reagent . the bio - synthesis of cannabinoids represents a landmark achievement in the field of composting , vector removal and ecological reconstitution . although the benefits have been known for millennia , the advent of modern bio - engineering techniques brings these small seeds of native wisdom to bear on a broader and more industrialized scale — removing dangerous molds and pestilences such as mosquitoes from swamped and flooded regions , raw sewage areas , and disaster sites where ensuing vermin and harmful vectors may cause greater damage than the initial catastrophes . it is the ambition and intention of authors that tactical usage of this broad - sweeping technique may rapidly and at low cost satisfy a global demand in what may be termed a “ grass - roots ” bio - engineering project worthy of the 3 rd millennium ; bringing to fruition micro - mass - productivity . should a clear error be found either in spirit or in factual evidence presented please do not hesitate to contact me .

Description:
polyketide synthesis converts 3 malonyl coa plus 1 n - hexanoyl - coa to form oscoa . this conversion may take place inside the muck and sludge , or may take place in a contained area and after the oscoa stilbene carboxylate synthase - like ( stcsl ), in the case of cannabis sativa a chalcone synthase ( chs ) that exhibits stilbene synthase ( sts ) activity in vivo and as per note in the literature ( raharjo 2004 ) there is reason to believe that the sequis used in the preferred embodiment of this invention and refers to any enzyme that generates 5 - amylresorcinolic acid ( olivetolic acid ). while there are several enzymes capable of synthesizing olivetolic acid in the final analysis any enzyme capable of olivetolic acid synthesis will is sufficient . in the preferred embodiment the stcsl is inserted into the mitochondrial genome using the protofection technique ( khan 2004 ). the stcsl should be operably linked to bioluminescent protein to facilitate the monitoring of activity . the vector of the stcsl should also , in the preferred embodiment , have an operably linked in an alternate embodiment of this invention olivetolic acid is synthesized through inorganic techniques and thus added to the filthy sludge as a trigger molecule . in this manner the invention would have a limiting reagent from the offset , restricting the final output of pestilence ridders in such cases wherein limitations might be preferable . in another alternate embodiment of the invention the stcsl is chimeric with goap , or a pestilence ridding molecule . geranylpyrophosphate olivetolic acid prenylase ( goap ) ( fellermeier 1998 ) is an integral part of this invention , and converts olivetolic acid into cannabigerolic acid . as one skilled in the art may appreciate any enzyme capable of yielding cannabigerolic acid is sufficient . in the preferred embodiment the goap is loaded into the vector in the manner described in fellermeier &# 39 ; s work . in the preferred embodiment of this invention the goap is operably linked to a bioluminescent protein such as gfp or aequorin , and thus its activation is more easily monitored with minimal technical expertise . the goap is also operably linked to a promoter capable of up - regulating goap and thereby amplifying goap production . products made from these transgenic vectors should produce thca , and , in addition , other precursor molecules as well as the necessary enzymes and proteins requisite for the aforementioned production , such as , tetrahydrocannibigerolic acid synthase , cannabigerolic acid synthase ( cbgas ), cannabidiolic acid synthase ( cbdas ), cannabichromenic acid synthase ( cbrmas ), tetrahydrocannibinolic acid ( thca ), olivetolic acid , polyketide synthase , and cannabigerolic acid synthase . also disclosed is the unique and novel application of the ttas in the function of a compost toilet additive and for the low - impact , sustainable , macrobiotic control of pests including alabama argillacea ( riley 1885 ), pieris brassicae ( beling 1932 ), melolontha melolontha ( mateeva 1995 ), and aphelenchoides composticola , ( grewal 1989 ). first the transgenically enhanced vectors ( tevs ) as necessary and leading up to the cannabigerolic acid phase of biosynthesis ( fig1 ) are added into the growth medium and let to rest for anywhere from 12 hours to several days with a temperature range of 25 - 35 degrees centigrade , and also depending on the volume of waste , the thickness of the muck , and the general nature of the festering filth . if time is of the essence one may speed up growth times by dispersing units of tevs around the afflicted region through artificial or assisted means . if precision in timing is desired it may be convenient to include a bioluminescent protein operably linked a functional promoter to the tevs similar in methods to ( uspto u . s . pat . no . 6 , 544 , 729 ) and created such as to reflect the activity of the tevs . next the transgenic plant vector is placed atop the festering sludge . the transgenic plant vector releases cannabinoids into the sludge , and as it appropriates greater the product of transgenic e . coli ( s ) so shall it release cannabinoids — all the while eradicating both the transgenic e . coli vector as well as the numerous pathogens , microbes , insects , fungi etc . . . that are defenseless against the cannabinoids . many of the attendant advantages of the invention become more readily apparent as the same become better understood by reference to the following detailed description , which taken with the accompanying drawing . fig1 provides a basic visual understanding for one skilled in the arts to process enzymatic thc , a drawing of the 3 phases of reaction . 25 . kane v v , razdan r k . constituents of hashish . a novel reaction of olivetol with citral in the presence of pyridine . total synthesis of dl - cannabicyclol and dl - cannabichromene . j am chem soc . 1968 nov . 6 ; 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