Patent Application: US-201615262529-A

Abstract:
a use of isomaltulose in the preparation of a vaginal composition , wherein the amount of isomaltulose used is 0 . 05 - 20 % . a vaginal composition contains 0 . 05 - 20 % of isomaltulose . the composition may be a health product , health care product , cleaning product , skin care product , deodorant , cosmetic , or disinfectant composition or a pharmaceutical composition . a non - therapeutic vaginal care method , a non - therapeutic vaginal cleaning method , a method for improving vaginal acidity , a method for promoting growth of lactobacillus in the vagina , and a method for preventing and treating vaginal dysbacteriosis , especially bacterial vaginal diseases , these methods being implemented by administering the composition to women &# 39 ; s vagina .

Description:
the composition was prepared with a method as follows : 9 . 0 g of isomaltulose , 0 . 05 g of sodium benzoate , 0 . 02 g of sodium dehydroacetate , 0 . 5 g of sodium propionate , and 2 . 7 g of xanthan gum were uniformly mixed . then 100 ml of distilled water was added to dissolve isomaltulose , sodium benzoate , sodium dehydroacetate and sodium propionate , stirred until uniform , upon which xanthan gum swelled and formed a uniform viscous gel . the gel was adjusted to ph 5 . 0 and sterilized at 112 . 6 ° c . for 20 min , and the water - soluble gel composition of the present invention was obtained . the gel was packed into individual , aseptic and sealed single dose package , 5 g per package . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 4 . 5 g , sodium dehydroacetate 0 . 02 g , sodium propionate 0 . 5 g , natamycin 2 . 5 mg , xanthan gum 2 . 6 g , and distilled water 100 ml , ph 5 . 0 . after sterilization , the gel was packed into individual , aseptic and sealed single dose package , 5 g per package . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 12 . 0 g , sodium benzoate 0 . 15 g , sodium dehydroacetate 0 . 01 g , sodium propionate 0 . 5 g , xanthan gum 2 . 5 g and distilled water 100 ml , ph 4 . 8 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 20 . 0 g , sodium benzoate 0 . 2 g , xanthan gum 3 . 0 g and distilled water 100 ml , ph 5 . 0 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 0 . 1 g , sodium benzoate 0 . 2 g , lauric acid 0 . 1 g , xanthan gum 2 . 5 g and distilled water 100 ml , ph 6 . 5 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 8 g , sodium caprate 0 . 1 g , lactoferrin 1 . 0 g , aloe extract 5 g , xanthan gum 2 . 5 g and distilled water 100 ml , ph 6 . 5 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 1 . 5 g , sodium propionate 0 . 375 g , sodium caprylate 0 . 15 g , lactoferrin 0 . 25 g , xanthan gum 2 . 5 g and distilled water 100 ml , ph 6 . 5 . the raw materials were weighed according to the amounts below : isomaltulose 45 . 0 g , isomaltose 443 . 5 g , sodium benzoate 1 . 5 g and lactobacillus acidophilus powder 10 g ( containing fructooligosaccharide and 5 × 10 10 cfu of viable bacteria ). the ingredients above were fully mixed until uniform , and packed into 1000 capsules , each capsule containing 45 mg of isomaltulose , 443 . 5 mg of isomaltose , 1 . 5 mg of sodium benzoate and 5 × 10 7 cfu of lactobacillus acidophilus . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 9 . 0 g , sodium propionate 0 . 45 g , sodium benzoate 0 . 05 g , sodium dehydroacetate 0 . 025 g , estriol 0 . 1 g , xanthan gum 3 . 0 g and water 87 . 375 g , ph 5 . 0 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 10 g , sodium benzoate 0 . 15 g , natamycin 2 mg , xanthan gum 3 . 0 g and distilled water 100 ml , ph 5 . 0 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 5 . 0 g , sodium benzoate 0 . 2 g , xanthan gum 2 . 5 g and distilled water 100 ml , ph 5 . 5 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 2 . 0 g , sodium propionate 0 . 7 g , xanthan gum 2 . 5 g and distilled water 94 . 8 g , ph 6 . 5 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 6 . 0 g , sodium propionate 0 . 5 g , sodium dehydroacetate 0 . 02 g , sodium caprylate 0 . 05 g , lactoferrin 0 . 5 g , natamycin 10 mg , total soy isoflavones 0 . 02 g , xanthan gum 2 . 5 g and distilled water 100 ml , ph 6 . 5 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 1 . 5 g , sodium dehydroacetate 0 . 025 g , xanthan gum 3 . 0 g and distilled water 100 ml , ph 6 . 5 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 8 . 5 g , sodium dehydroacetate 0 . 02 g , sodium caprylate 0 . 1 g , lactoferrin 0 . 5 g , xanthan gum 2 . 8 g and distilled water 100 ml , ph 6 . 5 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 8 . 0 g , sodium propionate 0 . 5 g , xanthan gum 2 . 8 g and distilled water 100 ml , ph 6 . 2 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 8 . 0 g , sodium propionate 0 . 5 g , sodium dehydroacetate 0 . 02 g , lactoferrin 0 . 5 g , xanthan gum 2 . 8 g and distilled water 100 ml , ph 6 . 2 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 9 . 0 g , sodium benzoate 0 . 2 g , fluconazole 0 . 2 g , xanthan gum 3 . 0 g and distilled water 100 ml , ph 6 . 5 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 1 . 5 g , sodium propionate 0 . 5 g , sodium dehydroacetate 0 . 025 g , xanthan gum 2 . 5 g and distilled water 100 ml , ph 5 . 4 . the raw materials were weighed according to the amounts below : isomaltulose 20 . 0 g , sodium propionate 0 . 5 g , sodium benzoate 0 . 05 g , sodium dehydroacetate 0 . 02 g , metronidazole 0 . 005 g , xanthan gum 2 . 5 g and distilled water 100 ml . isomaltulose , sodium propionate , sodium benzoate , sodium dehydroacetate and xanthan gum were uniformly mixed . then 90 ml of distilled water was added , stirred , stirred and heated , and sterilized . 10 ml of a sterilized metronidazole solution ( containing 0 . 005 g of metronidazole ) was added , adjusted to ph 5 . 0 and uniformly mixed . the raw materials were weighed according to the amounts below : isomaltulose 35 g , isomaltose 63 g , sodium benzoate 0 . 5 g , metronidazole 0 . 01 g , clotrimazole 0 . 01 g , magnesium stearate 1 . 48 g , uniformly mixed and tabletted . each tablet weighed 0 . 5 g and contained 175 mg of isomaltulose , 315 mg of isomaltose , 2 . 5 mg of sodium benzoate , 0 . 05 mg of metronidazole , 0 . 05 mg of clotrimazole and 7 . 4 mg of magnesium stearate . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : isomaltulose 10 g , benzoic acid 0 . 05 g , polycarbophil 2 . 0 g and distilled water 100 ml , ph 4 . 0 . the raw materials were weighed according to the amounts below , and the composition was prepared essentially following the method of example 1 : benzoic acid 0 . 15 g , isomaltulose 3 . 0 g , isomaltose 9 . 0 g , fluconazole 0 . 15 g , xanthan gum 2 . 5 g and distilled water 100 g , ph 5 . 4 . an aqueous solution of a composition was prepared according to the amounts and the method below . 12 g of isomaltulose and 0 . 2 g of sodium benzoate were weighed , added to 100 ml of distilled water , stirred , dissolved , adjusted to ph 5 . 0 and sterilized , and an aqueous solution of the composition is obtained . an aqueous solution of a composition was prepared according to the amounts and the method below . 12 g of isomaltulose , 0 . 5 g of sodium propionate , 0 . 02 g of sodium dehydroacetate , and 0 . 1 g of sodium caprylate were weighed , added into 100 ml of distilled water , stirred , dissolved , adjusted to ph 5 . 0 and sterilized . the benefits of the use of isomaltulose in the preparation and the preparation method provided in the present invention are described below with reference to following experimental examples . to observe the promotion of isomaltulose for acid production by bv - associated bacteria , and compare the promotion effect with that of isomaltose , sucrose and maltose . { circle around ( 1 )} isomaltulose group : containing 1 % ( w / v ) isomaltulose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 5 % ( w / v ) xanthan gum , ph5 . 4 ; { circle around ( 2 )} isomaltose group : containing 1 % ( w / v ) isomaltose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 5 % ( w / v ) xanthan gum , ph5 . 4 ; { circle around ( 3 )} sucrose group : containing 1 % ( w / v ) sucrose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 5 % ( w / v ) xanthan gum , ph5 . 4 ; and { circle around ( 4 )} maltose group : containing 1 % ( w / v ) maltose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 5 % ( w / v ) xanthan gum , ph5 . 4 . the gel of each group was formulated essentially following the method described in composition example 1 , then sterilized , added with calf serum , and packed in test tubes in a specification of 5 g / tube , for late use . ( 2 ) secretion specimens from bv patients : vaginal secretions from 5 bv patients ; and the criteria of inclusion are : { circle around ( 1 )} conforming to the amsel clinical criteria of bv diagnosis , wherein the ph value of the vaginal secretions was & gt ; 4 . 6 ; { circle around ( 2 )} after smearing , gram staining and microscopical examination of vaginal secretions , nugent score ≧ 7 ; and { circle around ( 3 )} no other vaginosis and no antimicrobial agent is used within two weeks . ( 3 ) after sampling , the secretion specimens from bv patients meeting the criteria of inclusion were immediately inoculated to the above 4 gels containing a carbohydrate , and slightly aerobically incubated at 37 ° c . for 46 hrs . the ph values of the gel matrices are detected with a precise ph test paper respectively at 14 hrs , 36 hrs , and 46 hrs . as shown in table 1 , the ph in each test tube declines from the initial value 5 . 4 to a value in the range of 3 . 8 - 5 . 1 after the secretion specimens from bv patients are incubated for 14 hrs in the 4 gels containing a carbohydrate , wherein the ph of the isomaltulose gel is the lowest , the ph of the sucrose gel is the highest and the ph of the isomaltose and maltose gels are in the middle . after 36 hr - incubation , the ph of the 4 gels containing a carbohydrate declines to below 4 . 6 , wherein the ph of the isomaltulose gel is the lowest and the ph of the sucrose gel is the highest . after 46 hr - incubation , the ph values of the 4 gels containing a carbohydrate are the same and ≦ 3 . 8 . in this example , after the carbohydrate containing gels are inoculated with the secretion specimens from bv patients , and slightly aerobically incubated for 14 hrs , the ph values in 4 of 5 isomaltulose gels decline to below 4 . 1 , the ph values in 4 of 5 isomaltose gels and in 4 of 5 maltose gels decline to below 4 . 4 and the ph values in 4 of 5 sucrose gel are still 4 . 4 or above . after continuous incubation for 36 hrs , the ph values in 3 of 3 isomaltulose gels decline to 3 . 8 or below , the ph values in 2 of 3 sucrose gels are still above 4 . 4 and the ph values in 2 of 3 isomaltose gels and 2 of 3 maltose gels decline to 3 . 8 or below . after continuous incubation for 46 hrs , the ph values in the 4 carbohydrate containing gels inoculated respectively with 2 specimens show no difference , and are ≦ 3 . 8 . the results show that through metabolization by bv - associated bacterial flora , the acid production from isomaltulose is faster than that from isomaltose and maltose , and especially faster than that from sucrose . the normal vaginal ph value is generally in the range of 3 . 5 to 4 . 5 , and the ph of vaginal secretions from bv patients is higher than 4 . 6 . the growth of various bacteria has a most suitable ph range . when the ph is 5 . 4 , the growth and metabolism to produce acids of neutrophilic bacteria are inhibited , while the acid resistant lactobacilli and bifidobacteria etc can still grow and produce acids . therefore , the ph of a lactobacillus selective medium is generally 5 . 4 . lactic acid is considered to play an important role in the maintenance of a normal vaginal bacterial flora , and protection against bv - associated bacterial flora and other pathogens [ 12 ] . in this example , the initial ph value of the carbohydrate containing gel is 5 . 4 . under such a ph condition , the carbohydrate is degraded by the bv - associated bacterial flora and primarily by the lactobacilli among them , to produce acids . the faster the decline in the ph value of the gel is , the faster the inhibitory effect on harmful bv - associated bacteria is , and the faster the modulation for bv - associated bacterial flora will be . it can be seen from the results of this example that the isomaltulose can be more easily degraded by bv - associated bacteria under slightly aerobic conditions to produce acids , so that isomaltulose is advantageous over isomaltose and maltose , and particularly obviously over sucrose . therefore , isomaltulose is more suitable for use in the preparation of vaginal compositions according to the present invention . to observe the promotion of isomaltulose for acid production by bv - associated bacteria , and compare the promotion effect with that of glucose , fructose , mannose , sucrose , maltose , and isomaltose . { circle around ( 1 )} glucose group : containing 1 . 5 % ( w / v ) glucose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 2 )} fructose group : containing 1 . 5 % ( w / v ) fructose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 3 )} mannose group : containing 1 . 5 % ( w / v ) mannose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 4 )} sucrose group : containing 1 . 5 % ( w / v ) sucrose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 5 )} maltose group : containing 1 . 5 % ( w / v ) maltose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 6 )} isomaltose group : containing 1 . 5 % ( w / v ) isomaltose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; and { circle around ( 7 )} isomaltulose group : containing 1 . 5 % ( w / v ) isomaltulose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 . the semi - solid agar of each group was formulated essentially following the method described in composition example 1 , then sterilized , added with calf serum , and packed in test tubes in a specification of 5 g / tube , for late use . ( 2 ) secretion specimens from bv patients : vaginal secretions from 5 bv patients ; and the criteria of inclusion are : { circle around ( 1 )} conforming to the amsel clinical criteria of bv diagnosis , wherein the ph value of the vaginal secretions was & gt ; 4 . 6 ; { circle around ( 2 )} after smearing , gram staining and microscopical examination of vaginal secretions , nugent score ≧ 7 ; and { circle around ( 3 )} no other vaginosis and no antimicrobial agent is used within two weeks . ( 3 ) after sampling , the secretion specimens from bv patients meeting the criteria of inclusion were immediately inoculated to the above 7 aseptic semi - solid agars containing a carbohydrate at the bedside and anaerobically incubated at 37 ° c . for 24 hrs . the ph values of the agar matrices are detected with a precise ph test paper . as shown in table 2 , after the secretion specimens from 5 bv patients are respectively anaerobically incubated for 24 hrs in 7 semi - solid agars containing a carbohydrate , the ph values of all the semi - solid agars decline from initial 5 . 4 to 3 . 8 - 4 . 6 . from low to high , the ph values of isomaltulose and maltose are the lowest , followed by isomaltose , then sucrose , then glucose , and finally fructose and mannose . in this example , after the 7 semi - solid agars are inoculated with the secretion specimens from 5 bv patients respectively and anaerobically incubated for 24 hrs , the ph values in each culture tube decline . the magnitude of decline reflects the efficiency of acid production through metabolization of various carbohydrates by bv - associated bacteria under anaerobic conditions . in general , the decline in the ph value of a disaccharide containing semi - solid agar is greater than that of a monosaccharide containing semi - solid agar . among the disaccharides , the isomaltulose and maltose containing semi - solid agars have a ph that declines more greatly than that of the sucrose and isomaltose containing semi - solid agars , indicating that in terms of the acid production through metabolization by bv - associated bacterial flora , disaccharide , particularly isomaltulose and maltose are faster than sucrose and isomaltose , and much faster than the monosaccharide . the normal vaginal ph value is generally in the range of 3 . 5 to 4 . 5 , and the ph of vaginal secretions from bv patients is higher than 4 . 6 . the growth of various bacteria has a most suitable ph range . when the ph is 5 . 4 , the growth and metabolism to produce acids of neutrophilic bacteria are inhibited , while the acid resistant lactobacilli and bifidobacteria etc can still grow and produce acids . therefore , the ph of a lactobacillus selective medium is generally 5 . 4 . lactic acid is considered to play an important role in the maintenance of a normal vaginal bacterial flora , and protection against bv - associated bacterial flora and other pathogens [ 12 ] . in this example , the initial ph value of the carbohydrate containing gel is 5 . 4 . under such a ph condition , the carbohydrate is degraded by the bv - associated bacterial flora and primarily by the lactobacilli among them , to produce acids . the faster the decline in the ph value of the gel is , the faster the inhibitory effect on harmful bv - associated bacteria is , and the faster the modulation for bv - associated bacterial flora will be . in summary , the results of this example show that isomaltulose is highly effectively in promoting the metabolism of vaginal bacterial flora to produce acids under anaerobic conditions , and is suitable for use in the preparation of vaginal compositions according to the present invention . to observe the combined effect of isomaltulose and an antibacterial agent on acid production by bv - associated bacteria , and compare the effect with that of sucrose and isomaltose . { circle around ( 1 )} sucrose - preservative group : containing 1 . 5 % ( w / v ) sucrose , 0 . 5 % ( w / v ) sodium propionate , 0 . 025 % ( w / v ) sodium dehydroacetate , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 2 )} isomaltose - preservative group : containing 1 . 5 % ( w / v ) isomaltose , 0 . 5 % ( w / v ) sodium propionate , 0 . 025 % ( w / v ) sodium dehydroacetate , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 3 )} isomaltulose - preservative group : containing 1 . 5 % ( w / v ) isomaltulose , 0 . 5 % ( w / v ) sodium propionate , 0 . 025 % ( w / v ) sodium dehydroacetate , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 4 )} sucrose control group : containing 1 . 5 % ( w / v ) sucrose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; { circle around ( 5 )} isomaltose control group : containing 1 . 5 % ( w / v ) isomaltose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 ; and { circle around ( 6 )} isomaltulose control group : containing 1 . 5 % ( w / v ) isomaltulose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph5 . 4 . the semi - solid agar of each group is formulated essentially following the method described in composition example 1 , then sterilized , added with calf serum , and packed in test tubes in a specification of 5 g / tube , for late use . ( 2 ) secretion specimens from bv patients : vaginal secretions from 2 bv patients ; and the criteria of inclusion are : { circle around ( 1 )} conforming to the amsel clinical criteria of bv diagnosis , wherein the ph value of the vaginal secretions was & gt ; 4 . 6 ; { circle around ( 2 )} after smearing , gram staining , and microscopical examination of vaginal secretions , nugent score ≧ 7 ; and { circle around ( 3 )} no other vaginosis , and no antimicrobial agent is used within two weeks . ( 3 ) after sampling , the vaginal secretion specimens from bv patients meeting the criteria of inclusion were immediately inoculated to 3 semi - solid agars containing a carbohydrate and a preservative and 3 control agars at the bedside , and anaerobically incubated at 37 ° c . for 40 hrs . the ph values of the agar matrices are detected with a precise ph test paper after 30 or 32 hrs and 40 or 48 hrs of incubation . as shown in table 3 , after 30 or 32 hrs of incubation , the ph values of the control semi - solid agars without antibacterial agent decline to 3 . 8 , or to 3 . 8 - 4 . 1 . after the semi - solid agars containing a carbohydrate and preservatives are anaerobically incubated for 30 or 32 hrs , the ph value of the semi - solid agar matrices declines , but is still higher than 4 . 4 . after 40 or 48 hrs of incubation , the ph value of the 2 isomaltulose containing semi - solid agar matrices is below 4 . 1 ; the ph value of the 2 sucrose containing semi - solid agar matrices is 4 . 1 and 4 . 4 respectively ; and the ph value of the 2 isomaltose containing semi - solid agar matrices is 4 . 4 and 4 . 6 respectively . after the three semi - solid agars containing a carbohydrate and the same preservatives are inoculated with specimens from bv patients , and incubated for 30 - 48 hrs , the ph value of the agar matrix in the isomaltulose group is the lowest , and declines to 3 . 8 and 3 . 8 - 4 . 1 at 40 and 48 hrs , which are significantly lower than the values 4 . 1 and 4 . 4 of the sucrose group , and the values 4 . 4 and 4 . 6 of the isomaltose group . the three control groups respectively contain 3 carbohydrates and no preservative . the ph of the agar matrices declines rapidly ; and the ph value in 5 of 6 agar matrices declines to 3 . 8 at 30 and 32 hrs , and to 3 . 8 - 4 . 1 in 1 of 6 agar matrices , which are significantly lower than the ph of the preservative containing agar matrices , indicating that the preservatives have great influence on the acid production of the vaginal bacteria . the influence on the acid production through metabolization of sucrose and isomaltose by the vaginal bacteria is most prominent . the acid production is reduced , the ph of the matrices declines slowly , and the ph value in 3 of 4 agar matrices is still 4 . 4 or above at 40 and 48 hrs , and is 4 . 1 in 1 of 4 agar matrices . the isomaltulose is also influenced by the preservatives , but to a lesser extent . on one hand , the ph of the agar matrix containing isomaltulose can decline to 3 . 8 or 3 . 8 - 4 . 1 at 40 and 48 hrs , while excessive acid production and unduly reduction of ph are avoided . the results obtained in this example show that the isomaltulose is obviously advantageous over sucrose and isomaltose , and is more suitable for use in combination with preservatives in the preparation of vaginal compositions containing preservatives and / or antibacterial agents according to the present invention . to observe the promotion of an isomaltulose gel for the growth of bv - associated bacteria , and compare the promotion effect with that of an isomaltose gel , a sucrose gel and a maltose gel . { circle around ( 1 )} isomaltulose group : containing 1 % ( w / v ) isomaltulose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 5 % ( w / v ) xanthan gum , ph5 . 4 ; { circle around ( 2 )} isomaltose group : containing 1 % ( w / v ) isomaltose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 5 % ( w / v ) xanthan gum , ph5 . 4 ; { circle around ( 3 )} sucrose group : containing 1 % ( w / v ) sucrose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 5 % ( w / v ) xanthan gum , ph5 . 4 ; and { circle around ( 4 )} maltose group : containing 1 % ( w / v ) maltose , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 5 % ( w / v ) xanthan gum , ph5 . 4 . the gel of each group was formulated essentially following the method described in composition example 1 , then sterilized , added with calf serum , and packed in test tubes in a specification of 5 g / tube , for late use . ( 2 ) secretion specimens from bv patients : vaginal secretions from 20 bv patients ; and the criteria of inclusion are : { circle around ( 1 )} conforming to the amsel clinical criteria of bv diagnosis ; { circle around ( 2 )} after smearing , gram staining and microscopical examination of vaginal secretions , nugent score ≧ 7 ; and { circle around ( 3 )} no other vaginosis and no antimicrobial agent is used within two weeks . ( 3 ) after sampling , the vaginal secretion specimens from bv patients meeting the criteria of inclusion were immediately inoculated to the above 4 gels containing a carbohydrate , slightly aerobically incubated at 37 ° c . for 48 hrs , then transferred to a plate containing improved mrs with 1 % ( w / v ) glucose and 5 % ( w / v ) calf serum , slightly aerobically incubated for 20 - 48 hrs , isolated , purified and multiplied . the strain was identified by 16s rdna sequencing . ( 1 ) as shown in table 4 , after the vaginal secretions from bv patients are incubated with 4 carbohydrate containing gels respectively , with the isomaltulose , isomaltose and sucrose gels , lactobacilli are cultured from the secretions derived from 9 bv patients ; and with the maltose gel , lactobacilli are cultured from the secretions derived from 8 bv patients , wherein no l . iners is cultured . for the secretions derived from 9 bv patients from which lactobacilli are cultured , there are 4 cases of l . jensenii , 2 cases of l . gasseri and each 1 case of l . crispatus , l . mucosae and l . amylovorus . this indicates that the 4 carbohydrate containing gels have a potent promotion for the growth of l . jensenii , l . gasseri and l . crispatus , and a weak promotion for the growth of l . iners . ( 2 ) as shown in table 5 , after the vaginal secretions from bv patients are incubated with 4 carbohydrate containing gels respectively , bifidobacterium bifidum is only cultured from the secretion derived from 1 bv patient with the maltose gel , and no bifidobacterium breve , bifidobacterium dentium and bifidobacterium longum is cultured from the remaining 3 gels , suggesting that the gels containing isomaltulose , isomaltose , and sucrose as a carbohydrate have a weak promotion for the growth of bifidobacteria commonly found in bv - associated flora . ( 3 ) as shown in table 6 , after the vaginal secretions from bv patients are incubated with 4 carbohydrate containing gels respectively , gardnerella vaginalis is cultured from the secretions of 6 bv patients with the maltose gel , from the secretions derived from 3 bv patients with the sucrose gel , and from the secretions derived from 2 bv patients with the isomaltulose and isomaltose gels , suggesting that the maltose gel facilitates the growth of gardnerella vaginalis . relevant studies [ 4 ] have found that the bv - associated bacterial flora is complex , and contains gardnerella vaginalis , prevotella , megacoccus , mobiluncus , peptostreptococcus , finegoldia , anaerococcus , and sneathia as preponderant bacteria , wherein the copies of 16s rrna gene sequence of gardnerella vaginalis rank first and account for 29 . 1 % of the total copies of 16s rrna gene sequences of the bacterial flora in the bv patients , which are over two times of the copies of 16s rrna gene sequence of prevotella that come in second ( 13 . 2 %), and three times of the copies of 16s rrna gene sequence of megasphaera that are in the third place ( 9 . 8 %). for the moderate bv , the copies of 16s rrna gene sequence of gardnerella vaginalis account for 30 . 5 % of the total copies of 16s rrna gene sequences of the vaginal bacterial flora in the patients with moderate bv , and are second to l . iners . it can be seen that gardnerella vaginalis is one of the most representative preponderant bacteria in the vaginal bacterial flora of bv patients . the lactobacilli in the bv - associated bacterial flora are reduced . the copies of 16s rrna gene sequence of l . iners account for 6 . 6 % of the total copies of 16s rrna gene sequences of the bacterial flora in the bv patients and are in the sixth place ; the value is only 0 . 3 % for l . crispatus ; and the value is even lower for l . jensenii and l . gasseri , which plus the value for other species of lactobacilli is only 0 . 2 % in total . for the moderate bv , the copies of 16s rrna gene sequence of l . iners rank first and account for 43 . 9 % of the total copies of 16s rrna gene sequences of the vaginal bacterial flora , the value for l . crispatus is 1 . 2 %, and the value for l . jensenii , l . gasseri and other lactobacilli is 6 . 1 % in total . in this example , after the vaginal secretions from 20 typical bv patients are incubated with 4 carbohydrate containing gels , the lactobacilli are cultured from the secretions of 9 bv patients with the isomaltulose , isomaltose and sucrose gels , and from the secretions of 8 bv patients with the maltose gel . the species of the cultured lactobacilli are primarily l . jensenii , and l . gasseri rarely found in the bv - associated bacterial flora , and secondarily l . crispatus , l . mucosae and l . amylovorus rarely found in the bv - associated bacterial flora . however , l . iners which is most frequently found in the bv - associated bacterial flora does not grow . these suggest that the gels containing isomaltulose , isomaltose , sucrose , and maltose are selective for the lactobacilli in the secretions from the bv patients , and have a potent promotion effect for the growth of l . jensenii , l . gasseri , and l . crispatus , and a weak promotion effect for the growth of l . iners . in the bv - associated bacterial flora , gardnerella vaginalis is the most prevelant . in this example , gardnerella vaginalis is cultured from the secretions of 6 bv patients with the maltose gel , from the secretions of 3 bv patients with the sucrose gel , and from the secretions of 2 bv patients with the isomaltulose and isomaltose gels , suggesting that the maltose gel is more suitable for the growth of gardnerella vaginalis , and the isomaltulose and isomaltose gels are relatively not suitable for the growth of gardnerella vaginalis . the bifidobacteria in the bv - associated bacterial flora are mostly bifidobacterium bifidum , bifidobacterium breve , bifidobacterium dentium , and bifidobacterium longum . in this example , bifidobacterium bifidum is cultured only from the secretion of 1 bv patient with the maltose gel , and no bifidobacteria grows with the isomaltulose , isomaltose and sucrose gels , suggesting that the isomaltulose , isomaltose , and sucrose have no effect of promoting the growth of bifidobacteria in the secretions of bv patients . 5 . conclusion : the isomaltulose , isomaltose , sucrose and maltose gels can all selectively promote the growth of l . jensenii , l . gasseri , l . crispatus , l . mucosae and l . amylovorus in the secretions of bv patients , but have no obvious promotion effect for the growth of l . iners . the isomaltulose , isomaltose and sucrose gels have no obvious promotion effect for the growth of bifidobacteria in the secretions of bv patients . the maltose gel may have a promotion effect for the growth of gardnerella vaginalis in the vaginal secretions of bv patients , and the promotion effect of the isomaltulose and isomaltose gels for the growth of gardnerella vaginalis is not obvious . in summary , the study results in this example suggest that in modulation of the bacterial flora , the isomaltulose is advantageous over maltose , and is more suitable for use in the preparation of vaginal compositions according to the present invention . to investigate the metabolization of isomaltulose by canidia albicans , and compare the metabolization with that of isomaltose , sucrose and maltose . isomaltulose group : containing 1 . 5 % ( w / v ) isomaltulose , mrs basal medium without carbohydrate , and 5 % ( w / v ) calf serum , ph 7 . 0 ; isomaltose group : containing 1 . 5 % ( w / v ) isomaltose , mrs basal medium without carbohydrate , and 5 % ( w / v ) calf serum , ph 7 . 0 ; sucrose group : containing 1 . 5 % ( w / v ) sucrose , mrs basal medium without carbohydrate , and 5 % ( w / v ) calf serum , ph 7 . 0 ; and maltose group : containing 1 . 5 % ( w / v ) maltose , mrs basal medium without carbohydrate , and 5 % ( w / v ) calf serum , ph7 . 0 . the culture medium of each group was strictly aseptically formulated , and packed in test tubes in a specification of 5 g / tube , for late use . ( 2 ) test strain : 2 strains of canidia albicans , isolated from patients with candidal vaginitis . ( 3 ) the bacterial solutions containing 2 strains of canidia albicans were aseptically inoculated into each test tube , aerobically incubated at 37 ° c . for 72 hrs , and sterilized at 80 ° c . for 30 min . the supernatant was separated by centrifugation , and packed in a specification of 5 ml / tube . the carbohydrate content in each tube was detected by hplc . as shown in table 7 , after the canidia albicans is incubated for 72 hrs with culture media containing 1 . 5 % ( w / v ) isomaltulose , isomaltose , sucrose and maltose respectively , the concentrations of the four carbohydrate decrease to different degrees , and the magnitude of decrease is noted in the sequence of maltose , isomaltulose , isomaltose and sucrose . the maltose is decreased from 1 . 5 % ( w / v ) to below 0 . 05 % ( w / v ). according to the report in relevant literatures , 85 % or more of the pathogens associated with candidal vaginitis is canidia albicans . after the two strains of canidia albicans are incubated for 72 hrs with the 4 carbohydrate containing culture media respectively , the residual concentration of maltose is below 0 . 05 % ( w / v ) for both strains , the residual concentration of isomaltulose is 0 . 11 % ( w / v ) for one strain and 0 . 12 % ( w / v ) for the other stain , the residual concentration of isomaltose is 0 . 74 % ( w / v ) for one strain and 0 . 76 % ( w / v ) for the other stain , and the residual concentration of sucrose is the highest , and is 1 . 04 % ( w / v ) for one strain and 0 . 95 % ( w / v ) for the other stain . these suggest that the four carbohydrates can all be metabolized by canidia albicans , wherein the metabolization capability is relatively highest for maltose , and relatively the lowest for sucrose . it can be seen from the study results in this example that the sucrose , isomaltose and isomaltulose are advantageous over maltose , and are more suitable for use in the preparation of vaginal compositions according to the present invention . to observe the combined effect of isomaltulose and preservatives on the growth of bv - associated bacteria . ( 1 ) isomaltulose agar : 1 . 5 % ( w / v ) isomaltulose , 0 . 05 % ( w / v ) sodium benzoate , 0 . 5 % ( w / v ) sodium propionate , 0 . 025 % ( w / v ) sodium dehydroacetate , mrs basal medium without carbohydrate , 5 % ( w / v ) calf serum , and 0 . 45 % ( w / v ) agar , ph 5 . 4 . the composition was formulated essentially following the method of composition example 1 , then sterilized , added with calf serum , and packed in test tubes in a specification of 5 g / tube , for late use . ( 2 ) secretion specimens of bv patients : vaginal secretions from 1 bv patients ; and criteria of inclusion : { circle around ( 1 )} conforming to the amsel clinical criteria of bv diagnosis , wherein the ph of the vaginal secretions was & gt ; 4 . 6 ; { circle around ( 2 )} after smearing , gram staining , and microscopical examination of vaginal secretions , nugent score ≧ 7 ; and { circle around ( 3 )} no other vaginosis , and no antimicrobial agent is used within two weeks . ( 3 ) after being sampled by a vaginal swab , the vaginal secretion specimens from a bv patient meeting the criteria of inclusion were washed off into a mrs basal medium without carbohydrate at bed side , immediately inoculated to the isomaltulose agar , and anaerobically incubated at 37 ° c . for 40 hrs . the agar matrix and the secretion specimen as a solution were shipped to sangon biotech ( shanghai ) co ., ltd ., for 16s rdna metagenome sequencing , and detecting the lactobacillus , l . crispatus , l . iners , l . jensenii , l . gasseri , gardnerella vaginalis and prevotella by qpcr . ( 1 ) as shown in table 8 , the bacteria in the vaginal secretions of this bv patient have sneathia ( 43 . 02 %), prevotella ( 19 . 44 %) and gardnerella ( 12 . 68 %) as preponderant bacteria . after inoculation to the isomaltulose agar and anaerobic incubation for 40 hrs , the lactobacilli account for 91 %, and the gardnerella vaginalis accounts for 6 %. ( 2 ) as shown in table 9 , the dna copies listed therein are respectively the bacterial dna copies contained in 0 . 12 ml of agar matrix and 0 . 12 specimen suspension . it is found through detection of 7 bacteria in the secretion specimen of the bv patient by qpcr that the number of the prevotella and gardnerella vaginalis is large ; the number of the lactobacillus , l . crispatus and l . iners is rare , the number of l . jensenii is extremely rare , and no l . gasseri is detected . it is found through detection of 7 bacteria in the isomaltulose agar by qpcr that the number of l . crispatus is the most prevelant , the number of l . jensenii is rare , and no l . gasseri is detected . the number of the l . iners and prevotella is reduced and the number of the gardnerella vaginalis is increased . the experimental results show that after the bv - associated bacteria are anaerobically incubated for 40 hrs in the isomaltulose agar containing antibacterial agents , the metagenome sequencing shows that lactobacilli grow largely and become preponderant bacteria , while the proportion of sneathia , prevotella and gardnerella in the flora decreases greatly . further detection by qpcr confirms that the preponderant lactobacilli are l . crispatus , the copies of the dna sequence of which are increased by 377494 times , from 2355 to 889000000 . however , the copies of the dna sequence of l . iners are reduced from 56296 to 33167 . the number of prevotella is reduced , and the number of l . jensenii is still very small . it is noted that the copies of the dna sequence of l . crispatus are more than the copies of the dna sequence of lactobacillus , which may be attributed to that the bacteria amplified with the primer for lactobacillus do not include all the bacteria belonging to the genus , and that the bacteria amplified with the primer for l . crispatus do not exclusively include l . crispatus , as supposed after discussion with the specialist from sangon biotech ( shanghai ) co ., ltd . the absolute number of gardnerella vaginalis is also increased by 4 . 27 times , which may be correlated with the ability to support the growth of gardnerella vaginalis by the calf serum contained in the agar matrix . the results obtained in this example show that the isomaltulose has a notable promotion effect for the growth of protective l . crispatus , and not for the growth of l . iners , suggesting that the isomaltulose and antibacterial agents in combination are suitable for the preparation of compositions according to the present invention . to observe the antibacterial effect of different preservatives on the candida and lactobacilli . the following culture media containing different concentrations of preservatives were formulated with a basal medium containing 0 . 5 % ( w / v ) isomaltulose , mrs medium without carbohydrate , and 5 % ( w / v ) calf serum : the culture medium of each group was adjusted to ph 5 . 4 , sterilized and packed in a test tube in a specification of 2 ml / tube . ( 2 ) inoculation and incubation of test strains : a bacterial suspension containing ( 1 - 5 )× 10 5 cfu / ml candida glabrada isolate and a bacterial suspension containing ( 1 - 5 )× 10 5 cfu / ml l . jensenii were inoculated to each tube respectively . the candida glabrada was aerobically incubated at 37 ° c . for 72 hrs , and the l . jensenii was microaerophilic incubated for 22 hrs . ( 3 ) observations : the turbidity in the l . jensenii incubation tube was observed at 22 hrs , and the turbidity in the candida glabrada incubation tube was observed at 72 hrs . 3 . test results as shown in table 10 , 0 . 1 % ( w / v ) sodium benzoate has a potent inhibition on lactobacilli , and can fully inhibit the growth of lactobacilli at 22 hrs . 0 . 02 % ( w / v ) sodium dehydroacetate and 0 . 5 % ( w / v ) sodium propionate has a weak inhibition on lactobacilli , as indicated by a greatly increased turbidity of lactobacilli at 22 hrs . when used alone , 0 . 2 % ( w / v ) sodium benzoate , 0 . 02 % ( w / v ) sodium dehydroacetate , and 0 . 5 % ( w / v ) sodium propionate show a weak inhibition on candida glabrada , as indicated by a greatly increased turbidity of candida glabrada at 72 hrs . the sodium dehydroacetate and sodium propionate have a synergistic inhibition on candida glabrada . when 0 . 02 % ( w / v ) sodium dehydroacetate and 0 . 5 % ( w / v ) sodium propionate are used in combination , the growth of candida glabrada can be completely inhibited at 72 hrs , with the turbidity being unchanged . however , the inhibition on l . jensenii is relatively weak , and the turbidity is increased at 22 hrs . the results obtained in this experimental example confirm that when used in combination , 0 . 02 % ( w / v ) sodium dehydroacetate and 0 . 5 % ( w / v ) sodium propionate has a higher inhibitive effect on candida glabrada than that of 0 . 2 % ( w / v ) sodium benzoate . when used in combination , 0 . 02 % ( w / v ) sodium dehydroacetate and 0 . 5 % ( w / v ) sodium propionate has a weaker inhibitive effect on l . jensenii than that of 0 . 2 % ( w / v ) sodium benzoate . two female rhesus monkeys were screened . the vaginal secretions of them had a ph value of 5 . 1 and 4 . 8 respectively , the vaginal bacteria were primarily gram negative bacilli and gram positive cocci , no gross gram positive bacilli were found , and the white cells were rare . 0 . 5 g of the isomaltulose gel obtained in composition example 1 was dosed once a day for 5 consecutive days . upon reexamination , the vaginal ph values were 3 . 8 and 4 . 1 respectively , and the vaginal bacteria were primarily gross gram positive bacilli . female , 28 years old , healthy , without unwell feeling , and had regular menstrual cycle . the vaginal secretions were removed with a vaginal swab 3 days after the completion of the menstrual cycle , and were tested to have a ph of 4 . 8 . after smearing , gram staining , and microscopical examination , more gross gram positive bacilli , some gram positive cocci , a few gram negative bacilli , and rare white cells were found . the woman voluntarily received the composition prepared in composition example 2 for vaginal cleaning and nursing , in a dosage of 3 g each application , for consecutive 2 days . the vaginal secretions were removed again with a vaginal swab , and were tested to have a ph of 4 . 1 . after smearing , gram staining , and microscopical examination , more gross gram positive bacilli were found , and the gram positive cocci and negative bacilli were reduced . the woman had a cleaner , comfortable , and refreshing feeling in the vagina . female , 52 years old , over 1 year after menopause , and often had a feeling of vaginal dryness and occasionally slight sting . the vaginal secretions were removed with a vaginal swab , and were tested to have a of ph 5 . 1 . after smearing , gram staining , and microscopical examination , it was found that few bacteria were present , which are mostly gram positive cocci , occasionally gram positive bacilli , and rarely white cells . the woman voluntarily received the composition prepared in composition example 13 for vaginal cleaning and nursing , in a dosage of 5 g each application , for consecutive 5 days . the vaginal secretions were removed again with a vaginal swab , and were tested to have a of ph 4 . 1 . after smearing , gram staining , and microscopical examination , more gross gram positive bacilli were found , while other types of bacteria are few . the woman had a feeling of comfort and no dryness and sting in the vagina . female , 35 years old , diagnosed of “ vaginitis ” in the hospital due to leukorrhagia and odor of leucorrhea , received treatment with “ shuangzuotaishuan ” comprising metronidazole , clotrimazole and chlorhexidine acetate , once a day for 5 consecutive days , and found that the leucorrhea was reduced and the odor disappeared . the vaginal secretions were removed with a vaginal swab , and tested to have a ph of 5 . 4 . after smearing , gram staining and microscopical examination , it was found that few bacteria were present , and no white cell was found . the woman voluntarily received the composition prepared in composition example 6 for vaginal cleaning and nursing , once a day in a dosage of 5 g each application , for consecutive 3 days . the vaginal secretions were removed again with a vaginal swab , and were tested to have a ph of 3 . 8 . after smearing , gram staining , and microscopical examination , more gross gram positive bacilli were found . the woman had a feeling of comfort and ease . female , 41 years old , with suspected “ vaginal dysbacteriosis ” from smear examination of vaginal secretion upon physical examination , but having no obvious discomfort usually . the vaginal secretions were removed with a vaginal swab , and were tested to have a ph of 4 . 6 . after smearing , gram staining , and microscopical examination , fewer gross gram positive bacilli and more gram positive cocci were found , small gram - variable bacilli were found , the nugent score was 5 , and there were few white cells , which conformed to the manifestation of moderate bv . the woman voluntarily received the composition prepared in composition example 17 for vaginal cleaning and nursing , once a day in a dosage of 5 g each application , for consecutive 5 days . upon reexamination , the ph value of the vaginal secretion was 3 . 8 , the vaginal bacteria were primarily gross gram positive bacilli . gram positive cocci and small gram negative bacilli were considerably reduced . the woman had a feeling of clean leucorrhea and freshness . female , 45 years old , with repeatedly reoccurring leukorrhagia accompanied by pruritus , wherein the symptoms were alleviated or disappeared after treatment with antibacterial agents , but often reoccurred after menstruation . the vaginal secretions were removed with a vaginal swab , and were tested to have a ph of 5 . 4 . after smearing , gram staining and microscopical examination , it was found that the gross gram positive bacilli were rare , the bacteria were primarily a variety of gram negative bacilli , gram negative cocci and gram positive cocci , and the white cells were few , which conformed to the manifestation of bacterial vaginosis . the woman voluntarily received the composition prepared in composition example 21 for vaginal cleaning and 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