Patent Application: US-201214005756-A

Abstract:
the present invention provides a composition comprising a broad spectrum protein of microbial origin as active anti - hiv / aids agent . either the protein is secreted by or surface associated in microorganisms including but not limiting to bacteria , both pathogenic and non - pathogenic . the proteins used are isolated from bacteria mycobacterium spp . specifically from mycobacterium tuberculosis or m . bovis bcg . further , the protein could be substituted by various truncated derivatives thereof , peptides derived from such proteins , synthetically prepared peptides , and proteins or peptides modified by pegylation , acetylation , and phosphorylation . the protein includes purified proteins and peptides having amino acid sequence of seq id no . 1 and 2 respectively .

Description:
1 . a method comprising administering to a patient who has hiv / aids a pharmaceutical composition consisting of one or more anti - hiv / aids compounds selected from the group consisting of mpt63 protein or various truncated derivatives of mpt63 protein , called peptides , all of which demonstrate anti - hiv / aids activity . the amino acid sequence of mpt63 protein is given under materials and methods . this compound may also be used in combination with other known anti - hiv / aids agents to enhance their potency . 2 . the method of embodiment 1 where the viruses can be selected from the group consisting of all clades of hiv / aids virus . of course , anti - viral activity of mpt63 protein , and peptides derived from it , can also be measured against other viruses such as hepatitis b and c viruses , dengue virus , measles virus , swine flu virus , polio virus , herpes simplex virus , japanese encephalitis virus and other viruses . 3 . the method of embodiment 2 where the hiv / aids viruses are contacted by the bacterial protein or peptides leading to virus killing , inhibition of virus entry or fusion with host cells , inhibition of virus propagation , blocking of gp - 120 epitope and other virus epitopes , blocking of host functions important in hiv / aids virus transport and / or entry into host t cells in hiv / aids patients or in other virus - infected patients . 4 . a method whereby such proteins or peptides are introduced in patients infected with any of the viruses mentioned in embodiment 2 . 5 . the method of introduction of the proteins / peptides in virus patients may involve intravenous ( iv ), intramuscular , oral , subcutaneous or topical application , in presence or absence of adjuvants or excipients . 6 . it is understood from common knowledge in protein chemistry that many of the amino acids can be replaced by other amino acids without loss of anti viral activity . thus the protein or peptide sequences can be variable by 10 to 40 % without any loss of activity . 7 . the proteins or peptides in embodiment 1 wherein the structure of the protein or peptide can be modified by pegylation , acetylation , phosphorylation , etc , to extend or optimize the half life of the protein or the peptide , or to reduce immunogenicity , in the patient bloodstream . such modifications may also lead to extended virus targets other than hiv - 1 such as polio , hepatitis b or c , dengue , h1n1 , and others . the mpt63 protein was purified to more than 95 % purity as per the protocol described in materials and methods and further used in the experiments . treatment of hiv / aids virus with mpt63 protein in this investigation revealed anti - hiv / aids property of the protein . all the experiments were carried out in triplicates and repeated three times . as shown in fig2 , the replication of hiv - 1 nl4 - 3 is competitively inhibited by mpt63 similar to enfuvirtide ( t20 ), with a ec50 of 10 − 2 nm . the effect of mpt63 in a larger number of primary isolates of hiv / aids is further evaluated . in order to check the effect of mpt63 protein in different clades of hiv / aids viruses , mpt63 protein was incubated with clade c and clade g of hiv / aids virus at the concentrations mentioned in materials and methods section . as shown in fig3 , the replication of primary isolates of clade c and g was competitively inhibited by mpt63 at various concentrations . the clade g of hiv - 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