Patent Application: US-82015504-A

Abstract:
the present invention pertains to the use of subunits and oligomers of collectins and / or ficolins , such as mannan - binding lectin in prophylactic and / or curative treatment of severe acute respiratory syndrome in an individual .

Description:
sars may be prevented and / or treated in individuals independent on their serum collectin and / or ficolin level , such as mbl level . the collectin according to the invention may be any collectin capable of preventing or treating sars in an individual . accordingly , the collectin may be selected from the group consisting of mbl ( mannose - binding lectin ), sp - a ( lung surfactant protein a ), sp - d ( lung surfactant protein d ), bk ( or bc , bovine conglutinin ), cl - l1 ( ohtani et al . 1999 , molecular cloning of a novel human collectin from liver ( cl - l1 ), j . biol . chem . 274 : 13681 - 89 ), cl - p1 ( ohtani et al . 2001 . the membrane - type collectin cl - p1 is a scavenger receptor on vascular endothelial cells . j . biol . chem . 276 : 44222 - 28 ), and cl43 ( collectin - 43 ). most preferably the collectin is mbl . ( holmskov et al . 2003 , annu rev . immunol . 21 : 547 - 78 ). in a particular preferred embodiment the collectin has one of the sequences listed below with reference to their database and accession no . 1 : q9npy3 complement component c1q receptor precursor ( complement component 1 , q subcomponent , receptor 1 ) ( c1qrp ) ( c1qr ( p )) ( c1q / mbl / spa receptor ) ( cd93 antigen ) ( cdw93 ) gi | 21759074 | sp | q9npy3 | cd93_human [ 21759074 ] 2 : bac05523 collectin placenta 1 [ mus musculus ] gi | 21901969 | dbj | bac05523 . 1 |[ 21901969 ] 3 : aam34743 46 - kda collectin precursor [ bos taurus ] gi | 21105687 | gb | aam34743 . 1 | af509590_1 [ 21105687 ] 4 : aam34742 46 - kda collectin precursor [ bos taurus ] gi | 21105685 | gb | aam34742 . 1 | af509589_1 [ 21105685 ] 5 : xp_139613 similar to collectin sub - family member 10 ; collectin liver 1 ; collectin 34 [ mus musculus ] gi | 20903807 | ref | xp_139613 . 1 |[ 20903807 ] 6 : xp_123211 similar to collectin sub - family member 12 [ mus musculus ] gi | 20876566 | ref | xp_123211 . 1 |[ 20876566 ] 7 : np_571645 mannose binding - like lectin [ danio rerio ] gi | 18858997 | ref | np_571645 . 1 |[ 18858997 ] 8 : np_569057 collectin sub - family member 12 , isoform i ; scavenger receptor with c - type lectin ; collectin placenta 1 [ homo sapiens ] gi | 18641360 | ref | np_569057 . 1 |[ 18641360 ] 9 : np_110408 collectin sub - family member 12 , isoform ii ; scavenger receptor with c - type lectin ; collectin placenta 1 [ homo sapiens ] gi | 18641358 | ref | np_110408 . 2 |[ 18641358 ] 10 : np_569716 collectin sub - family member 12 [ mus musculus ] gi | 18485494 | ref | np_569716 . 1 |[ 18485494 ] 11 : aal61856 43 kda collectin precursor [ bos taurus ] gi | 18252111 | gb | aal61856 . 1 |[ 18252111 ] 12 : aal61855 43 kda collectin precursor [ bos taurus ] gi | 18252109 | gb | aal61855 . 1 |[ 18252109 ] 13 : bab22581 data source : sptr , source key : q9y6z7 , evidence : iss ˜ homolog to collectin 34 ˜ putative [ mus musculus ] gi | 12833584 | dbj | bab22581 . 1 |[ 12833584 ] 14 : np_034905 mannose binding lectin , liver ( a ) [ mus musculus ] gi | 6754654 | ref | np_034905 . 1 |[ 6754654 ] 15 : np_034906 mannose binding lectin , serum ( c ) [ mus musculus ] gi | 6754656 | ref | np_034906 . 1 |[ 6754656 ] 16 : np_006429 collectin sub - family member 10 ; collectin liver 1 ; collectin 34 [ homo sapiens ] gi | 5453619 | ref | np_006429 . 1 |[ 5453619 ] 17 : bab72147 collectin placenta 1 [ homo sapiens ] gi | 17026101 | dbj | bab72147 . 1 |[ 17026101 ] 18 : aaf63470 mannose binding - like lectin precursor [ carassius auratus ] gi | 7542474 | gb | aaf63470 . 1 | af227739_1 [ 7542474 ] 19 : aaf63469 mannose binding - like lectin precursor [ danio rerio ] gi | 7542472 | gb | aaf63469 . 1 | af227738_1 [ 7542472 ] 20 : aaf63468 mannose binding - like lectin precursor [ cyprinus carpio ] gi | 7542470 | gb | aaf63468 . 1 | af227737_1 [ 7542470 ] 21 : aak97540 surfactant protein a precursor [ gallus gallus ] gi | 15420996 | gb | aak97540 . 1 | af411083_1 [ 15420996 ] 22 : lnmsmc mannose - binding lectin c precursor - mouse gi | 7428747 | pir || lnmsmc [ 7428747 ] 23 : lnmsma mannose - binding lectin a precursor - mouse gi | 625320 | pir || lnmsma [ 625320 ] 24 : jn0450 conglutinin precursor - bovine gi | 346501 | pir || jn0450 [ 346501 ] 25 : a57250 mannan - binding protein - chicken ( fragment ) gi | 1362725 | pir || a57250 [ 1362725 ] 26 : a53570 collectin - 43 - bovine gi | 1083017 | pir || a53570 [ 1083017 ] 27 : aaf28384 lung surfactant protein a [ sus scrofa ] gi | 6782434 | gb | aaf28384 . 1 | af133668_1 [ 6782434 ] 28 : aaf22145 lung surfactant protein d precursor ; spd ; sp - d ; cp4 [ sus scrofa ] gi | 6760482 | gb | aaf22145 . 2 | af132496_1 [ 6760482 ] 29 : p41317 mannose - binding protein c precursor ( mbp - c ) ( mannan - binding protein ) ( ra - reactive factor p28a subunit ) ( rarf / p28a ) gi | 1346477 | sp | p41317 | mabc_mouse [ 1346477 ] 30 : p39039 mannose - binding protein a precursor ( mbp - a ) ( mannan - binding protein ) ( ra - reactive factor polysaccharide - binding component p28b polypeptide ) ( rarf p28b ) gi | 729972 | sp | p39039 | maba_mouse [ 729972 ] 31 : p42916 collectin - 43 ( cl - 43 ) gi | 1168967 | sp | p42916 | cl43_bovin [ 1168967 ] 32 : cab56155 dmbt1 / 8kb . 2 protein [ homo sapiens ] gi | 5912464 | emb | cab56155 . 1 |[ 5912464 ] 33 : baa81747 collectin 34 [ homo sapiens ] gi | 5162875 | dbj | baa81747 . 1 |[ 5162875 ] 34 : aab94071 mannan - binding lectin ; collectin [ gallus gallus ] gi | 2736145 | gb | aab94071 . 1 |[ 2736145 ] 35 : aab36019 mannan - binding protein , mbp = lectin { n - terminal } [ chickens , serum , peptide partial , 30 aa ] [ gallus gallus ] gi | 1311692 | gb | aab36019 . 1 |[ 1311692 ] 36 : aab27504 conglutinin ( n ) { n - terminal } [ cattle , peptide partial , 60 aa ] [ bos taurus ] gi | 386660 | gb | aab27504 . 1 |[ 386660 ] 37 : caa53511 collectin - 43 [ bos taurus ] gi | 499385 | emb | caa53511 . 1 |[ 499385 ] 38 : aaa82010 mannose - binding protein c [ mus musculus ] gi | 773288 | gb | aaa82010 . 1 |[ 773288 ] 39 : aaa82009 mannose - binding protein a [ mus musculus ] gi | 773280 | gb | aaa82009 . 1 |[ 773280 ] 1 : 1kmra chain a , solution nmr structure of surfactant protein b ( 11 - 25 ) ( sp - b11 - 25 ) gi | 22219056 | pdb | 1kmr | a [ 22219056 ] 2 : p50404 pulmonary surfactant - associated protein d precursor ( sp - d ) ( psp - d ) gi | 1709879 | sp | p50404 | pspd_mouse [ 1709879 ] 3 : p06908 pulmonary surfactant - associated protein a precursor ( sp - a ) ( psp - a ) ( psap ) gi | 1172693 | sp | p06908 | pspa_canfa [ 1172693 ] 4 : p35247 pulmonary surfactant - associated protein d precursor ( sp - d ) ( psp - d ) gi | 464486 | sp | p35247 | pspd_human [ 464486 ] 5 : p12842 pulmonary surfactant - associated protein a precursor ( sp - a ) ( psp - a ) ( psap ) gi | 131413 | sp | p12842 | pspa_rabit [ 131413 ] 6 : np_033186 surfactant associated protein d [ mus musculus ] gi | 6677921 | ref | np_033186 . 1 |[ 6677921 ] 7 : 1b08c chain c , lung surfactant protein d ( sp - d ) ( fragment ) gi | 6573321 | pdb | 1b08 | c [ 6573321 ] 8 : 1b08b chain b , lung surfactant protein d ( sp - d ) ( fragment ) gi | 6573320 | pdb | 1b08 | b [ 6573320 ] 9 : 1b08a chain a , lung surfactant protein d ( sp - d ) ( fragment ) gi | 6573319 | pdb | 1b08 | a [ 6573319 ] 10 : np_060049 deleted in malignant brain tumors 1 isoform c precursor [ homo sapiens ] gi | 8923740 | ref | np_060049 . 1 |[ 8923740 ] 11 : np_015568 deleted in malignant brain tumors 1 isoform b precursor [ homo sapiens ] gi | 6633801 | ref | np_015568 . 1 |[ 6633801 ] 12 : np_004397 deleted in malignant brain tumors 1 isoform a precursor [ homo sapiens ] gi | 4758170 | ref | np_004397 . 1 |[ 4758170 ] 13 : lnboc1 pulmonary surfactant protein c - bovine gi | 7428752 | pir || lnboc1 [ 7428752 ] 14 : lndgc1 pulmonary surfactant protein c - dog gi | 7428750 | pir || lndgc1 [ 7428750 ] 15 : jn0450 conglutinin precursor - bovine gi | 346501 | pir || jn0450 [ 346501 ] 16 : a45225 pulmonary surfactant protein d precursor - human gi | 346375 | pir || a45225 [ 346375 ] 17 : lnhuc pulmonary surfactant protein c precursor , long splice form - human gi | 71983 | pir || lnhuc [ 71983 ] 18 : lndgps pulmonary surfactant protein a precursor - dog gi | 71970 | pir || lndgps [ 71970 ] 19 : lnhups pulmonary surfactant protein a precursor ( genomic clone ) - human gi | 71967 | pir || lnhups [ 71967 ] 20 : a53570 collectin - 43 - bovine gi | 1083017 | pir || a53570 [ 1083017 ] 21 : s33603 surfactant protein d - bovine gi | 423283 | pir || s33603 [ 423283 ] 22 : aaf28384 lung surfactant protein a [ sus scrofa ] gi | 6782434 | gb | aaf28384 . 1 | af133668_1 [ 6782434 ] 23 : aaf22145 lung surfactant protein d precursor ; spd ; sp - d ; cp4 [ sus scrofa ] gi | 6760482 | gb | aaf22145 . 2 | af132496_1 [ 6760482 ] 24 : p15783 pulmonary surfactant - associated protein c ( sp - c ) ( pulmonary surfactant - associated proteolipid spl ( val )) gi | 131422 | sp | p15783 | pspc_bovin [ 131422 ] 25 : p35246 pulmonary surfactant - associated protein d precursor ( sp - d ) ( psp - d ) gi | 464485 | sp | p35246 | pspd_bovin [ 464485 ] 26 : p42916 collectin - 43 ( cl - 43 ) gi | 1168967 | sp | p42916 | cl43_bovin [ 1168967 ] 27 : cab56155 dmbt1 / 8kb . 2 protein [ homo sapiens ] gi | 5912464 | emb | cab56155 . 1 |[ 5912464 ] 28 : aad49696 gp - 340 variant protein [ homo sapiens ] gi | 5733598 | gb | aad49696 . 1 | af159456_1 [ 5733598 ] 29 : aad31380 surfactant protein d precursor [ mus musculus ] gi | 4877556 | gb | aad31380 . 1 | af047742_1 [ 4877556 ] 30 : b61249 pulmonary surfactant protein c - dog gi | 539712 | pir || b61249 [ 539712 ] 31 : s00609 pulmonary surfactant protein c - bovine gi | 89749 | pir || s00609 [ 89749 ] 32 : a43628 pulmonary surfactant protein a - human ( fragments ) gi | 280854 | pir || a43628 [ 280854 ] 33 : aab48076 surfactant protein b ( sp - b ) [ oryctolagus cuniculus ] gi | 1850933 | gb | aab48076 . 1 |[ 1850933 ] 34 : 1901176a surfactant protein a gi | 382753 | prf || 1901176a [ 382753 ] 35 : caa53510 lung surfactant protein d [ bos taurus ] gi | 415939 | emb | caa53510 . 1 |[ 415939 ] 36 : caa53511 collectin - 43 [ bos taurus ] gi | 499385 | emb | caa53511 . 1 |[ 499385 ] 37 : caa46152 lung surfactant protein d [ homo sapiens ] gi | 34767 | emb | caa46152 . 1 |[ 34767 ] 38 : aaa92788 lung surfactant protein c [ rattus norvegicus ] gi | 595282 | gb | aaa92788 . 1 |[ 595282 ] 39 : aaa31468 surfactant protein a [ oryctolagus cuniculus ] gi | 431446 | gb | aaa31468 . 1 |[ 431446 ] 1 : q9npy3 complement component c1q receptor precursor ( complement component 1 , q subcomponent , receptor 1 ) ( c1qrp ) ( c1qr ( p )) ( c1q / mbl / spa receptor ) ( cd93 antigen ) ( cdw93 ) gi | 21759074 | sp | q9npy3 | cd93_human [ 21759074 ] 2 : o89103 complement component c1q receptor precursor ( complement component 1 , q subcomponent , receptor 1 ) ( c1qrp ) ( c1qr ( p )) ( c1q / mbl / spa receptor ) ( cd93 antigen ) ( cell surface antigen aa4 ) ( lymphocyte antigen 68 ) gi | 21541998 | sp | o89103 | cd93_mouse [ 21541998 ] 3 : p09871 complement c1s component precursor ( c1 esterase ) gi | 115205 | sp | p09871 | c1s_human [ 115205 ] 4 : np_036204 complement component 1 , q subcomponent , receptor 1 ; complement component c1q receptor [ homo sapiens ] gi | 6912282 | ref | np_036204 . 1 |[ 6912282 ] 5 : np_000233 soluble mannose - binding lectin precursor ; mannose - binding lectin ; mannose binding protein ; mannose - binding lectin 2 , soluble ( opsonic defect ) [ homo sapiens ] gi | 4557739 | ref | np_000233 . 1 |[ 4557739 ] 6 : aam94381 lectin precursor [ zephyranthes candida ] gi | 22212748 | gb | aam94381 . 1 | af527385_1 [ 22212748 ] 7 : aah21762 mannose binding lectin , liver ( a ) [ mus musculus ] gi | 18256010 | gb | aah21762 . 1 |[ 18256010 ] 8 : aah10760 similar to mannose binding lectin , serum ( c ) [ mus musculus ] gi | 14789670 | gb | aah10760 . 1 |[ 14789670 ] 9 : p11226 mannose - binding protein c precursor ( mbp - c ) ( mbp1 ) ( mannan - binding protein ) ( mannose - binding lectin ) gi | 126676 | sp | p11226 | mabc_human [ 126676 ] 10 : np_034897 mannan - binding lectin serine protease 2 [ mus musculus ] gi | 6754642 | ref | np_034897 . 1 |[ 6754642 ] 11 : q9et61 complement component c1q receptor precursor ( complement component 1 , q subcomponent , receptor 1 ) ( c1qrp ) ( c1qr ( p )) ( c1q / mbl / spa receptor ) ( cd93 antigen ) ( cell surface antigen aa4 ) gi | 21541989 | sp | q9et61 | cd93_rat [ 21541989 ] 12 : np_006601 mannan - binding lectin serine protease 2 , isoform 1 precursor ; mbl - associated plasma protein of 19 kd ; small mbl - associated protein [ homo sapiens ] gi | 21264363 | ref | np_006601 . 2 |[ 21264363 ] 13 : np_631947 mannan - binding lectin serine protease 2 , isoform 2 precursor ; mbl - associated plasma protein of 19 kd ; small mbl - associated protein [ homo sapiens ] gi | 21264361 | ref | np_631947 . 1 |[ 21264361 ] 14 : np_624302 mannan - binding lectin serine protease 1 , isoform 2 , precursor ; protease , serine , 5 ( mannose - binding protein - associated ); manan - binding lectin serine protease - 1 ; ra - reactive factor serine protease p100 [ homo sapiens ] gi | 21264359 | ref | np_624302 . 1 |[ 21264359 ] 15 : np_001870 mannan - binding lectin serine protease 1 , isoform 1 , precursor ; protease , serine , 5 ( mannose - binding protein - associated ); manan - binding lectin serine protease - 1 ; ra - reactive factor serine protease p100 [ homo sapiens ] gi | 21264357 | ref | np_001870 . 3 |[ 21264357 ] 16 : xp_122683 similar to mannose binding lectin , liver ( a ) [ mus musculus ] gi | 20872845 | ref | xp_122683 . 1 |[ 20872845 ] 17 : aam21196 c - type mannose - binding lectin [ oncorhynchus mykiss ] gi | 20385163 | gb | aam21196 . 1 | af363271_1 [ 20385163 ] 18 : aad45377 mannose - binding lectin [ sus scrofa ] gi | 5566370 | gb | aad45377 . 1 | af164576_1 [ 5566370 ] 19 : np_034905 mannose binding lectin , liver ( a ) [ mus musculus ] gi | 6754654 | ref | np_034905 . 1 |[ 6754654 ] 20 : np_034906 mannose binding lectin , serum ( c ) [ mus musculus ] gi | 6754656 | ref | np_034906 . 1 |[ 6754656 ] 21 : aal14428 dendritic cell - specific icam - 3 grabbing nonintegrin [ macaca nemestrina ] gi | 16118455 | gb | aal14428 . 1 | af343727_1 [ 16118455 ] 22 : aaf63470 mannose binding - like lectin precursor [ carassius auratus ] gi | 7542474 | gb | aaf63470 . 1 | af227739_1 [ 7542474 ] 23 : aaf63469 mannose binding - like lectin precursor [ danio rerio ] gi | 7542472 | gb | aaf63469 . 1 | af227738_1 [ 7542472 ] 24 : aaf63468 mannose binding - like lectin precursor [ cyprinus carpio ] gi | 7542470 | gb | aaf63468 . 1 | af227737_1 [ 7542470 ] 25 : aaf21018 mannose - binding lectin 2 [ sus scrofa ] gi | 6644342 | gb | aaf21018 . 1 | af208528_1 [ 6644342 ] 26 : aak30298 mannose - binding lectin precursor protein [ gallus gallus ] gi | 13561409 | gb | aak30298 . 1 |[ 13561409 ] 27 : lnmsmc mannose - binding lectin c precursor - mouse gi | 7428747 | pir || lnmsmc [ 7428747 ] 28 : lnmsma mannose - binding lectin a precursor - mouse gi | 625320 | pir || lnmsma [ 625320 ] 29 : lnrtma mannose - binding lectin a precursor - rat gi | 71975 | pir || lnrtma [ 71975 ] 30 : lnrtmc mannose - binding lectin c precursor - rat gi | 71974 | pir || lnrtmc [ 71974 ] 31 : lnhumc mannose - binding lectin precursor - human gi | 71973 | pir || lnhumc [ 71973 ] 32 : baa86864 complement c1s [ homo sapiens ] gi | 6407558 | dbj | baa86864 . 1 |[ 6407558 ] 33 : p49329 mannose - specific lectin ( agglutinin ) gi | 1346426 | sp | p49329 | lec_aloar [ 1346426 ] 34 : cab56124 mannose - binding lectin [ homo sapiens ] gi | 5911809 | emb | cab56124 . 1 |[ 5911809 ] 35 : cab56123 mannose - binding lectin [ homo sapiens ] gi | 5911807 | emb | cab56123 . 1 |[ 5911807 ] 36 : cab56122 mannose - binding lectin [ homo sapiens ] gi | 5911798 | emb | cab56122 . 1 |[ 5911798 ] 37 : cab56121 mannose - binding lectin [ homo sapiens ] gi | 5911796 | emb | cab56121 . 1 |[ 5911796 ] 38 : cab56045 mannose - binding lectin [ homo sapiens ] gi | 5911794 | emb | cab56045 . 1 |[ 5911794 ] 39 : cab56120 mannose - binding lectin [ homo sapiens ] gi | 5911792 | emb | cab56120 . 1 |[ 5911792 ] 40 : cab56044 mannose - binding lectin [ homo sapiens ] gi | 5911790 | emb | cab56044 . 1 |[ 5911790 ] 41 : aab53110 c1qr ( p ) [ homo sapiens ] gi | 2052498 | gb | aab53110 . 1 |[ 2052498 ] the collectin preferably comprises at least 10 , such as at least 12 , for example at least 15 , such as at least 20 , for example at least 25 , such as at least 30 , for example at least 35 , such as at least 40 , for example at least 50 consecutive amino acid residues of the collectin or of a variant or a homologue to said collectin . such a variant or homologue is preferably at least 70 %, such as 80 %, for example 90 %, such as 95 % identical to the collectin . the ficolin according to the invention may be l - ficolin , h - ficolin or m - ficolin or variants or homologues thereof . in a preferred embodiment the ficolin is l - ficolin . in a particular preferred embodiment the ficolin has one of the sequences listed below with reference to their database and accession no . for each of the sequences the cystein rich region and the collagen - like region is described . np_003656 . ficolin 3 precursor ; ficolin ( collagen / fibrinogen domain - containing ) 3 ( hakata antigen ) [ homo sapiens ] [ gi : 4504331 ] 90 . . . 299 / region_name = “ pfam00147 , fibrinogen_c , fibrinogen beta and gamma chains , c - terminal globular domain ” 90 . . . 299 / region_name = “ smart00186 , fbg , fibrinogen - related domains ( freds ); domain present at the c - termini of fibrinogen beta and gamma chains , and a variety of fibrinogen - related proteins , including tenascin and drosophila scabrous ” 1 mdllwilpsl wllllggpac lktqehpscp gpreleaskv vllpscpgap gspgekgapg 61 pqgppgppgk mgpkgepgdp vnllrcqegp rncrellsqg atlsgwyhlc lpegralpvf 121 cdmdtegggw lvfqrrqdgs vdffrswssy ragfgnqese fwlgnenlhq ltlqgnwelr 181 veledfngnr tfahyatfrl lgevdhyqla lgkfsegtag dslslhsgrp fttydadhds 241 snsncavivh gawwyascyr snlngryavs daaahkygid wasgrgvghp yrrvrmmlr xp_116792 . similar to ficolin 2 precursor ( collagen / fibrinogen domain - containing protein 2 ) ( ficolin - b ) ( ficolin b ) ( serum lectin p35 ) ( ebp - 37 ) ( hucolin ) ( l - ficolin ) [ homo sapiens ] [ gi : 20477458 ] 91 . . . 168 / region_name = “ pfam00147 , fibrinogen_c , fibrinogen beta and gamma chains , c - terminal globular domain ” 91 . . . 168 / region_name = “ smart00186 , fbg , fibrinogen - related domains ( freds ); domain present at the c - termini of fibrinogen beta and gamma chains , and a variety of fibrinogen - related proteins , including tenascin and drosophila scabrous ” 1 mgpallalsf lwtmaltedt cpamleyval nsepgmaskn psrrhglsll vvdqgpgarg 61 vrtdqgpsga dpgslelhge cpifpseqvi lthhnnypfs tedqdndrda encavhyqga 121 wwyaschlsh lngvylggar dsftnginwk sgkgnnysyk vsemkvrpt o00602 . ficolin 1 precursor ( collagen / fibrinogen domain - containing protein 1 ) ( ficolin - a ) ( ficolin a ) ( m - ficolin ) [ gi : 20455484 ] 1 . . . 29 / gene = “ fcn1 ”/ region_name = “ signal ”/ note = “ potential .” 30 . . . 326 / gene = “ fcn1 ”/ region_name = “ mature chain ”/ note = “ ficolin 1 .” 55 . . . 93 / gene = “ fcn1 ”/ region_name = “ domain ”/ note = “ collagen - like .” 133 / gene = “ fcn1 ”/ region_name = “ conflict ”/ note = “ t −& gt ; n ( in ref . 1 ).” 144 . . . 290 / gene = “ fcn1 ”/ region_name = “ domain ”/ note = “ fibrinogen c - terminal .” 287 / gene = “ fcn1 ”/ region_name = “ conflict ”/ note = “ n −& gt ; s ( in ref . 1 ).” 305 / gene = “ fcn1 ”/ site_type = “ glycosylation ”/ note = “ n - linked ( glcnac . . . ) ( potential ).“ 1 melsgatmar glavllvlfl hiknlpaqaa dtcpevkvvg legsdkltil rgcpglpgap 61 gpkgeagvig ergerglpga pgkagpvgpk gdrgekgmrg ekgdagqsqs catgprnckd 121 lldrgyflsg whtiylpdcr pltvlcdmdt dgggwtvfqr rmdgsvdfyr dwaaykqgfg 181 sqlgefwlgn dnihaltaqg sselrvdlvd fegnhqfaky ksfkvadeae kyklvlgafv 241 ggsagnsltg hnnnffstkd qdndvsssnc aekfqgawwy adchasnlng lylmgphesy 301 anginwsaak gykysykvse mkvrpa // o75636 . ficolin 3 precursor ( collagen / fibrinogen domain - containing protein 3 ) ( collagen / fibrinogen domain - containing lectin 3 p35 ) ( hakata antigen ) [ gi : 13124185 ] 1 . . . 21 / gene = “ fcn3 ”/ region_name = “ signal ”/ note = “ potential .” 22 . . . 299 / gene = “ fcn3 ”/ region_name = “ mature chain ”/ note = “ ficolin 3 .” 48 . . . 80 / gene = “ fcn3 ”/ region_name = “ domain ”/ note = “ collagen - like .” 50 / gene = “ fcn3 ”/ site_type = “ hydroxylation ” 53 / gene = “ fcn3 ”/ site_type = “ hydroxylation ” 59 / gene = “ fcn3 ”/ site_type = “ hydroxylation ” 65 / gene = “ fcn3 ”/ site_type = “ hydroxylation ” 68 / gene = “ fcn3 ”/ site_type = “ hydroxylation ” 77 / gene = “ fcn3 ”/ site_type = “ hydroxylation ” 119 . . . 265 / gene = “ fcn3 ”/ region_name = “ domain ”/ note = “ fibrinogen c - terminal .” 189 / gene = “ fcn3 ”/ site_type = “ glycosylation ”/ note = “ n - linked ( glcnac . . . ) ( potential ).” 1 mdllwilpsl wllllggpac lktqehpscp gpreleaskv vllpscpgap gspgekgapg 61 pqgppgppgk mgpkgepgdp vnllrcqegp rncrellsqg atlsgwyhlc lpegralpvf 121 cdmdtegggw lvfqrrqdgs vdffrswssy ragfgnqese fwlgnenlhq ltlqgnwelr 181 veledfngnr tfahyatfrl lgevdhyqla lgkfsegtag dslslhsgrp fttydadhds 241 snsncavivh gawwyascyr snlngryavs daaahkygid wasgrgvghp yrrvrmmlr xp_130120 . similar to ficolin 2 precursor ( collagen / fibrinogen domain - containing protein 2 ) ( ficolin - b ) ( ficolin b ) ( serum lectin p35 ) ( ebp - 37 ) ( hucolin ) [ mus mus - culus ] [ gi : 20823464 ] 59 . . . 95 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 59 . . . 89 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 60 . . . 95 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 60 . . . 95 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 60 . . . 95 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 60 . . . 95 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 60 . . . 95 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 61 . . . 95 / region name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 61 . . . 95 / region name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 61 . . . 95 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 103 . . . 312 / region_name = “ fibrinogen beta and gamma chains , c - terminal globular domain ”/ note = “ fibrinogen_c ”/ db_xref = “ cdd : pfam00147 ” 103 . . . 312 / region_name = “ fibrinogen - related domains ( freds )”/ note = “ fbg ”/ db_xref = “ cdd : smart00186 ” 1 malgsaalfv ltltvhaagt cpelkvldle gykqltilqg cpglpgaagp kgeagakgdr 61 gesglpgipg kegptgpkgn qgekgirgek gdsgpsqsca tgprtckell tqghfltgwy 121 tiylpdcrpl tvlcdmdtdg ggwtvfqrrl dgsvdffrdw tsykrgfgsq lgefwlgndn 181 ihalttqgts elrvdlsdfe gkhdfakyss fqiqgeaeky klilgnflgg gagdsltphn 241 nrlfstkdqd ndgstsscam gyhgawwysq chtsnlngly lrgphksyan gvnwkswrgy 301 nysckvsemk vrli np_056654 . ficolin 2 isoform d precursor ; ficolin ( collagen / fibrinogen domain - containing lectin ) 2 ( hucolin ); ficolin ( collagen / fibrinogen domain - containing lectin ) 2 ; hucolin [ homo sapiens ] [ gi : 8051590 ] 39 . . . 95 / region_name = “ collagen - like domain ” 1 meldravgvl gaatlllsfl gmawalqaad tcpevkmvgl egsdkltilr gcpglpgapg 61 dkgeagtngk rgergppgpp gkagppgpng apgepqpclt gd np_056653 . ficolin 2 isoform c precursor ; ficolin ( collagen / fibrinogen domain - containing lectin ) 2 ( hucolin ); ficolin ( collagen / fibrinogen domain - containing lectin ) 2 ; hucolin [ homo sapiens ] [ gi : 8051588 ] 39 . . . 95 / region_name = “ collagen - like domain ” 102 . . . 143 / region_name = “ fibrinogen beta and gamma chains , c - terminal globular domain ”/ note = “ fibrinogen_c ”/ db_xref = “ cdd : pfam00147 ” 102 . . . 143 / region_name = “ fibrinogen - related domains ( freds )”/ note = “ fbg ”/ db_xref = “ cdd : smart00186 ” 1 meldravgvl gaatlllsfl gmawalqaad tcpevkmvgl egsdkltilr gcpglpgapg 61 dkgeagtngk rgergppgpp gkagppgpng apgepqpclt gprtckdlld rghflsgwht 121 iylpdcrplt vlcdmdtdgg gwtvsvglgr ggqpgspggq aahlvgehtl efsillvgds 181 qr np_056652 . ficolin 2 isoform b precursor ; ficolin ( collagen / fibrinogen domain - containing lectin ) 2 ( hucolin ); ficolin ( collagen / fibrinogen domain - containing lectin ) 2 ; hucolin [ homo sapiens ] [ gi : 8051586 ] sig_peptide 1 . . . 25 mat_peptide 26 . . . 275 60 . . . 275 / region_name = “ fbg domain ”/ note = “ fibrinogen beta / gamma homology ” 64 . . . 275 / region_name = “ fibrinogen - related domains ( freds )”/ note = “ fbg ”/ db_xref = “ cdd : smart00186 ” 64 . . . 274 / region_name = “ fibrinogen beta and gamma chains , c - terminal globular domain ”/ note = “ fibrinogen_c ”/ db_xref = “ cdd : pfam00147 ” 1 meldravgvl gaatlllsfl gmawalqaad tcpgergppg ppgkagppgp ngapgepqpc 61 ltgprtckdl ldrghflsgw htiylpdcrp ltvlcdmdtd gggwtvfqrr vdgsvdfyrd 121 watykqgfgs rlgefwlgnd nihaltaqgt selrvdlvdf ednyqfakyr sfkvadeaek 181 ynlvlgafve gsagdsltfh nnqsfstkdq dndlntgnca vmfqgawwyk nchvsnlngr 241 ylrgthgsfa nginwksgkg ynysykvsem kvrpa np_001994 . ficolin 1 precursor ; ficolin ( collagen / fibrinogen domain - containing ) 1 [ homo sapiens ] [ gi : 8051584 ] sig_peptide 1 . . . 27 mat_peptide 28 . . . 326 40 . . . 108 / region_name = “ collagen - like domain ” 50 . . . 105 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 51 . . . 107 / region name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 52 . . . 106 / region_name = “ collagen triple helix repeat ( 20 copies )”/ note = “ collagen ”/ db_xref = “ cdd : pfam01391 ” 115 . . . 326 / region_name = “ fbg domain ”/ note = “ fibrinogen beta / gamma homology ” 115 . . . 326 / region_name = “ fibrinogen - related domains ( freds )”/ note = “ fbg ”/ db_xref = “ cdd : smart00186 ” 115 . . . 325 / region_name = “ fibrinogen beta and gamma chains , c - terminal globular domain ”/ note = “ fibrinogen c ”/ db_xref = “ cdd : pfam00147 ” variation 315 / db_xref = “ dbsnp : 1128428 ” variation 316 / db_xref = “ dbsnp : 1128429 ” variation 317 / db_xref = “ dbsnp : 1128430 ” 1 melsgatmar glavllvlfl hiknlpaqaa dtcpevkvvg legsdkltil rgcpglpgap 61 gpkgeagvig ergerglpga pgkagpvgpk gdrgekgmrg ekgdagqsqs catgprnckd 121 lldrgyflsg whtiylpdcr pltvlcdmdt dgggwtvfqr rmdgsvdfyr dwaaykqgfg 181 sqlgefwlgn dnihaltaqg sselrvdlvd fegnhqfaky ksfkvadeae kyklvlgafv 241 ggsagnsltg hnnnffstkd qdndvsssnc aekfqgawwy adchasnlng lylmgphesy 301 anginwsaak gykysykvse mkvrpa np_004099 . ficolin 2 isoform a precursor ; ficolin ( collagen / fibrinogen domain - containing lectin ) 2 ( hucolin ); ficolin ( collagen / fibrinogen domain - containing lectin ) 2 ; hucolin [ homo sapiens ] [ gi : 4758348 ] sig_peptide 1 . . . 25 mat_peptide 26 . . . 313 39 . . . 95 / region_name = “ collagen - like domain ” 98 . . . 313 / region_name = “ fbg domain ”/ note = “ fibrinogen beta / gamma homology ” 102 . . . 313 / region_name = “ fibrinogen - related domains ( freds )”/ note = “ fbg ”/ db_xref = “ cdd : smart00186 ” 102 . . . 312 / region_name = “ fibrinogen beta and gamma chains , c - terminal globular domain ”/ note = “ fibrinogen_c ”/ db_xref = “ cdd : pfam00147 1 meldravgvl gaatlllsfl gmawalqaad tcpevkmvgl egsdkltilr gcpglpgapg 61 dkgeagtngk rgergppgpp gkagppgpng apgepqpclt gprtckdlld rghflsgwht 121 iylpdcrplt vlcdmdtdgg gwtvfqrrvd gsvdfyrdwa tykqgfgsrl gefwlgndni 181 haltaqgtse lrvdlvdfed nyqfakyrsf kvadeaekyn lvlgafvegs agdsltfhnn 241 qsfstkdqdn dlntgncavm fqgawwyknc hvsnlngryl rgthgsfang inwksgkgyn 301 ysykvsemkv rpa q9wts8 . ficolin 1 precursor ( collagen / fibrinogen domain - containing protein 1 ) ( ficolin - a ) ( ficolin a ) ( m - ficolin ) [ gi : 13124116 ] 1 . . . 22 / gene = “ fcn1 ”/ region_name = “ signal ”/ note = “ potential .” 23 . . . 335 / gene =“ fcn1 ”/ region_name = “ mature chain ”/ note = “ ficolin 1 .” 50 . . . 88 / gene = “ fcn1 ”/ region_name = “ domain ”/ note = “ collagen - like .” 152 . . . 298 / gene = “ fcn1 ”/ region_name = “ domain ”/ note = “ fibrinogen c - terminal .” 271 / gene = “ fcn1 ”/ site_type = “ glycosylation ”/ note = “ n - linked ( glcnac . . . ) ( potential ).” 1 mwwpmlwafp vllclcssqa lgqesgacpd vkivglgaqd kvaviqscps fpgppgpkge 61 pgspagrger glqgspgkmg ppgskgepgt mgppgvkgek gergtasplg qkelgdalcr 121 rgprsckdll trgifltgwy tiylpdcrpl tvlcdmdvdg ggwtvfqrrv dgsinfyrdw 181 dsykrgfgnl gtefwlgndy lhlltangnq elrvdlrefq gqtsfakyss fqvsgeqeky 241 kltlgqfleg tagdsltkhn nmafsthdqd ndtnggknca alfhgawwyh dchqsnlngr 301 ylpgshesya dginwlsgrg hrysykvaem kiras q15485 . ficolin 2 precursor ( collagen / fibrinogen domain - containing protein 2 ) ( ficolin - b ) ( ficolin b ) ( serum lectin p35 ) ( ebp - 37 ) ( hucolin ) ( l - ficolin ) [ gi : 13124203 ] 1 . . . 25 / gene = “ fcn2 ”/ region_name = “ signal ”/ note = “ potential .” 26 . . . 313 / gene = “ fcn2 ”/ region_name = “ mature chain ”/ note = “ ficolin 2 .” 54 . . . 92 / gene = “ fcn2 ”/ region_name = “ domain ”/ note = “ collagen - like .” 131 . . . 277 / gene = “ fcn2 ”/ region_name = “ domain ”/ note = “ fibrinogen c - terminal ” 240 / gene = “ fcn2 ”/ site_type = “ glycosylation ”/ note = “ n - linked ( glcnac . . . ) ( potential ).” 300 / gene = “ fcn2 ”/ site_type = “ glycosylation ”/ note = “ n - linked ( glcnac . . . ) ( potential ).” 1 meldravgvl gaatlllsfl gmawalqaad tcpevkmvgl egsdkltilr gcpglpgapg 61 dkgeagtngk rgergppgpp gkagppgpng apgepqpclt gprtckdlld rghflsgwht 121 iylpdcrplt vlcdmdtdgg gwtvfqrrvd gsvdfyrdwa tykqgfgsrl gefwlgndni 181 haltaqgtse lrvdlvdfed nyqfakyrsf kvadeaekyn lvlgafvegs agdsltfhnn 241 qsfstkdqdn dlntgncavm fqgawwyknc hvsnlngryl rgthgsfang inwksgkgyn 301 ysykvsemkv rpa o70497 . ficolin 2 precursor ( collagen / fibrinogen domain - containing protein 2 ) ( ficolin - b ) ( ficolin b ) ( serum lectin p35 ) ( ebp - 37 ) ( hucolin ) [ gi : 13124181 ] & lt ; 1 . . . 15 / gene = “ fcn2 ”/ region_name = “ signal ”/ note = “ potential .” 16 . . . & gt ; 306 / gene = “ fcn2 ”/ region_name = “ mature chain ”/ note = “ ficolin 2 .” 41 . . . 79 / gene = “ fcn2 ”/ region_name = “ domain ”/ note = “ collagen - like .” 130 . . . 276 / gene = “ fcn2 ”/ region_name = “ domain ”/ note = “ fibrinogen c - terminal .” 299 / gene = “ fcn2 ”/ site_type = “ glycosylation ”/ note = “ n - linked ( glcnac . . . ) ( potential ).” 1 lgsaalfvlt ltvhaagtcp elkvldlegy kqltilqgcp glpgaagpkg eagakgdrge 61 sglpgipgke gptgpkgnqg ekgirgekgd sgpsqscatg prtckelltq ghfltgwyti 121 ylpdcrpmtv lcdmdtdggg wtvfqrrldg svdffrdwts ykrgfgsqlg efwlgndnih 181 alttqgtsel rvdlsdfegk hdfakyssfq iqgeaekykl ilgnflggga gdsltphnnr 241 lfstkdqdnd gstsscamgy hgawwysqch tsnlnglylr gphksyangv nwkswrgyny 301 sckvse o70165 . ficolin 1 precursor ( collagen / fibrinogen domain - containing protein 1 ) ( ficolin - a ) ( ficolin a ) ( m - ficolin ) [ gi : 13124179 ] 1 . . . 22 / gene = “ fcn1 ”/ region_name = “ signal ”/ note = “ potential .” 23 . . . 334 / gene = “ fcn1 ”/ region_name = “ mature chain ”/ note = “ ficolin 1 .” 50 . . . 88 / gene = “ fcn1 ”/ region_name = “ domain ”/ note = “ collagen - like .” 152 . . . 298 / gene = “ fcn1 ”/ region_name = “ domain ”/ note = “ fibrinogen c - terminal ” 261 / gene = “ fcn1 ”/ site_type = “ glycosylation ”/ note = “ n - linked ( glcnac . . . ) ( potential ).” 1 mqwptlwafs gllclcpsqa lgqergacpd vkvvglgaqd kvvviqscpg fpgppgpkge 61 pgspagrger gfqgspgkmg pagskgepgt mgppgvkgek gdtgaapslg ekelgdtlcq 121 rgprsckdll trgifltgwy tihlpdcrpl tvlcdmdvdg ggwtvfqrrv dgsidffrdw 181 dsykrgfgnl gtefwlgndy lhlltangnq elrvdlqdfq gkgsyakyss fqvseeqeky 241 kltlgqfleg tagdsltkhn nmsftthdqd ndansmncaa lfhgawwyhn chqsnlngry 301 lsgshesyad ginwgtgqgh hysykvaemk iras p57756 . ficolin 2 precursor ( collagen / fibrinogen domain - containing protein 2 ) ( ficolin - b ) ( ficolin b ) ( serum lectin p35 ) ( ebp - 37 ) ( hucolin ) [ gi : 13124114 ] 1 . . . 22 / gene = “ fcn2 ”/ region_name = “ signal ”/ note = “ potential .” 23 . . . 319 / gene = “ fcn2 ”/ region_name = “ mature chain ”/ note = “ ficolin 2 .” 48 . . . 86 / gene = “ fcn2 ”/ region_name = “ domain ”/ note = “ collagen - like .” 137 . . . 283 / gene = “ fcn2 ”/ region_name = “ domain ”/ note = “ fibrinogen c - terminal .” 306 / gene = “ fcn2 ”/ site_type = “ glycosylation ”/ note = “ n - linked ( glcnac . . . ) ( potential ).” 1 mvlgsaalfv lslcvteltl haadtcpevk vldlegsnkl tilqgcpglp galgpkgeag 61 akgdrgesgl pghpgkagpt gpkgdrgekg vrgekgdtgp sqscatgprt ckelltrgyf 121 ltgwytiylp dcrpltvlcd mdtdgggwtv fqrridgtvd ffrdwtsykq gfgsqlgefw 181 lgndnihalt tqgtnelrvd ladfdgnhdf akyssfqiqg eaekyklilg nflgggagds 241 ltsqnnmlfs tkdqdndqgs sncavryhga wwysdchtsn lnglylrglh ksyangvnwk 301 swkgynysyk vsemkvrli jc5980 . ficolin - a precurs - mouse [ gi : 7513652 ] 1 . . . 21 / region_name = “ domain ”/ note = “ signal sequence ” 50 . . . 64 / region_name = “ domain ”/ note = “ collagen - like ” 68 . . . 106 / region_name = “ domain ”/ note = “ collagen - like ” 123 . . . 334 / region_name = “ domain ”/ note = “ fibrinogen beta / gamma homology # label fbg ” 1 mqwptlwafs gllclcpsqa lgqergacpd vkvvglgaqd kvvviqscpg fpgppgpkge 61 pgspagrger gfqgspgkmg pagskgepgt mgppgvkgek gdtgaapslg ekelgdtlcq 121 rgprsckdll trgifltgwy tihlpdcrpl tvlcdmdvdg ggwtvfqrrv dgsidffrdw 181 dsykrgfgnl gtefwlgndy lhlltangnq elrvdlqdfq gkgsyakyss fqvseeqeky 241 kltlgqfleg tagdsltkhn nmsftthdqd ndansmncaa lfhgawwyhn chqsnlngry 301 lsgshesyad ginwgtgqgh hysykvaemk iras s61517 . ficolin - 1 precurs - human [ gi : 2135116 ] 1 . . . 326 / note = “ 36k hla - cross - reactive plasma protein ; hucolin , 35 k ” 1 . . . 22 / region_name = “ domain ”/ note = “ signal sequence ” 52 . . . 108 / region_name = “ region ”/ note = “ collagen - like ” 115 . . . 326 / region_name = “ domain ”/ note = “ fibrinogen beta / gamma homology # label fbg ” 305 / site_type = “ binding ”/ note = “ carbohydrate ( asn ) ( covalent )” 1 melsgatmar glavllvlfl hiknlpaqaa dtcpevkvvg legsdkltil rgcpglpgap 61 gpkgeagvig ergerglpga pgkagpvgpk gdrgekgmrg ekgdagqsqs catgprnckd 121 lldrgyflsg whniylpdcr pltvlcdmdt dgggwtvfqr rmdgsvdfyr dwaaykqgfg 181 sqlgefwlgn dnihaltaqg sselrvdlvd fegnhqfaky ksfkvadeae kyklvlgafv 241 ggsagnsltg hnnnffstkd qdndvsssnc aekfqgawwy adchasslng lylmgphesy 301 anginwsaak gykysykvse mkvrpa a47172 . transforming growth factor - beta 1 - binding protein homolog ficolin - alpha - pig [ gi : 423206 ] 112 . . . 323 / region_name = “ domain ”/ note = “ fibrinogen beta / gamma homology # label fbg ” 1 mdtrgvaaam rplvllvafl ctaapaldtc pevkvvgleg sdklsilrgc pglpgaagpk 61 geagasgpkg gqgppgapge pgppgpkgdr gekgepgpkg esweteqclt gprtckellt 121 rghilsgwht iylpdcqplt vlcdmdtdgg gwtvfqrrsd gsvdfyrdwa aykrgfgsql 181 gefwlgndhi haltaqgtne lrvdlvdfeg nhqfakyrsf qvadeaekym lvlgafvegn 241 agdsltshnn slfttkdqdn dqyasncavl yqgawwynsc hvsnlngryl ggshgsfang 301 vnwssgkgyn ysykvsemkf rat jc4942 . ficolin - 1 precursor - human [ gi : 2135117 ] 1 . . . 22 / region_name = “ domain ”/ note = “ signal sequence ” 45 . . . 101 / region_name = “ region ”/ note = “ collagen - like ” 108 . . . 319 / region_name = “ domain ”/ note = “ fibrinogen beta / gamma homology # label fbg ” 111 . . . 315 / region_name = “ region ”/ note = “ fibrinogen - like ” 298 / site_type = “ binding ”/ note = “ carbohydrate ( asn ) ( covalent )” 1 marglavllv lflhiknlpa qaadtcpevk vvglegsdkl tilrgcpglp gapgpkgeag 61 vigergergl pgapgkagpv gpkgdrgekg mrgekgdagq sqscatgprn ckdlldrgyf 121 lsgwhtiylp dcrpltvlcd mdtdgggwtv fqrrmdgsvd fyrdwaaykq gfgsqlgefw 181 lgndnihalt aqgsselrvd lvdfegnhqf akyksfkvad eaekyklvlg afvggsagns 241 ltghnnnffs tkdqdndvss sncaekfqga wwyadchasn lnglylmgph esyanginws 301 aakgykysyk vsemkvrpa aaf44911 . symbol = bg : ds00929 . . . [ gi : 7287873 ] 1 mkscffvlfl wtllfevgqs sphtcpsgsp ngihqlmlpe eepfqvtqck ttardwiviq 61 rrldgsvnfn qswfsykdgf gdpngeffig lqklylmtre qphelfiqlk hgpgatvyah 121 fddfqvdset elyklervgk ysgtagdslr yhinkrfstf drdndesskn caaehgggww 181 fhsclsr the ficolin preferably comprises at least 10 , such as at least 12 , for example at least 15 , such as at least 20 , for example at least 25 , such as at least 30 , for example at least 35 , such as at least 40 , for example at least 50 consecutive amino acid residues of the ficolins identified above or of a variant or a homologue to said ficolin . such a variant or homologue is preferably at least 70 %, such as 80 %, for example 90 %, such as 95 % identical to the complement activating protein . in the following the invention is described in relation to mbl as an example : sars may be prevented when administering mbl to these individuals having an mbl level in excess of 10 ng / ml serum . also , individuals having an mbl level in excess of 50 ng / ml serum may be in need of treatment , such as individuals having an mbl level in excess of 100 ng / ml serum , and individuals having an mbl level in excess of 150 ng / ml serum . also the mbl treatment of sars may be conducted by administering mbl to these individuals in combination with relevant antibiotics , anti - viral agents or anti - fungal agents . in particular , individuals at risk of acquiring sars will benefit from being prophylactically treated with mbl . generally all individuals exposed to sars patients should be treated with mbl independent on their specific mbl level . the reason behind this is that sars may lead to mbl depletion , and therefore an mbl “ booster ”, increasing the mbl level initially will reduce the risk of mbl depletion to a level below a deficiency level , and the immune defence of these patients can be reinforced by administration of recombinant or natural plasma - derived mbl . in particular sars may be prevented when administering mbl to individuals having an mbl level in excess of 10 ng / ml serum . also , individuals having an mbl level in excess of 50 ng / ml serum may be in need of treatment , such as individuals having an mbl level in excess of 100 ng / ml serum , and individuals having an mbl level in excess of 150 ng / ml serum . the present inventors have also shown herein that in particular individuals having an mbl level below 500 ng / ml serum will benefit from the mbl treatment . consequently , in particular individuals having an mbl level below 400 ng / ml will benefit , such as individuals having an mbl level below 300 ng / ml , such as individuals having an mbl level below 250 ng / ml , such as individuals having an mbl level below 200 ng / ml . thus , in a preferred embodiment the present invention relates to the use of mbl for manufacturing of a medicament for treatment of individuals having an mbl level in serum in the range of 10 - 500 ng / ml , such as in the range of 50 - 500 ng / ml for treating and / or preventing sars . one group of individuals being in need of mbl treatment in order to prevent and / or treat sars are individuals having a low level of functional mbl , independent on the level of mbl as such . this is due to the fact that for some mutations of the mbl it has been found that although mbl subunits and oligomers thereof are expressed in serum the functionality thereof are low . the functionality or functional activity of mbl may be estimated by its capacity to form an mbl / masp complex leading to activation of the complement system . when c4 is cleaved by mbl / masp an active thiolester is exposed and c4 becomes covalently attached to nearby nucleophilic groups . a substantial part of the c4b will thus become attached to the coated plastic well and may be detected by anti - c4 antibody . a quantitative trifma for mbl functional activity is constructed by 1 ) coating microtitre wells with 1 mg mannan in 100 ml buffer ; 2 ) blocking with tween - 20 ; 3 ) applying test samples , e . g . diluted mbl preparations 4 ) applying mbl deficient serum ( this leads to the formation of the mbl / masp complex ); alternatively the mbl and the mbl deficient serum may be mixed before application with the microtitre wells ; 5 ) applying purified complement factor c4 at 5 mg / ml ; 6 ) incubating for one hour at 37 ° c . ; 7 ) applying eu - labelled anti - c4 antibody ; 8 ) applying enhancement solution ; and 9 ) reading the eu by time resolved fluorometry . between each step the plate is incubated at room temperature and washed , except between step 8 and 9 . estimation by elisa may be carried out similarly , e . g . by applying biotin - labelled anti - c4 in step 7 ; 8 ) apply alkaline phosphatase - labelled avidin ; 9 ) apply substrate ; and 10 ) read the colour intensity . the functionality may be expressed as the specific activity of mbl , such as 1 unit of mbl activity per ng mbl . a non - functional mbl may be defined as mbl having a specific activity less than 50 % of plasma mbl specific activity , such as less than 25 % of plasma mbl specific activity , wherein the plasma mbl is purified from an individual not suffering from any mbl mutations . in particular the reference plasma mbl is plasma pool lj 6 . 57 apr . 28 , 1997 . thus , the present invention also relates to the prevention and / or treatment of sars in individuals having a mutation in their mbl gene leading to a reduced expression of mbl and / or expression of non - functional mbl . in particular such mutations in the mbl gene can lead to a change of aminoacid number 52 ( numbering including the leader peptide of mbl ) from arginine to cysteine , aminoacid number 54 from glycine to aspartic acid or amino acid number 75 from glycine to glutamic acid . also mutations in the promoter region of the mbl gene can lead to lowered levels of mbl . in particular mutations at position − 221 have an influence on the expression of mbl . the mbl sequence may be found in swiss . prot under accession no : 11226 . the mbl composition used to manufacture an mbl medicament may be produced from any mbl source available . the mbl source may be natural mbl , whereby the mbls are produced in a native host organism , meaning that mbl is produced by a cell normally expressing mbl . one usual method of producing an mbl composition is by extraction of mbl from human body liquids , such as serum or plasma , but mbl may also be harvested from cultures of hepatocytes . in another aspect the mbl oligomers are produced by a host organism not natively expressing an mbl polypeptide , such as by recombinant technology . in a first embodiment the mbl source may be serum , from which an mbl composition is obtained by purification from serum , plasma , milk product , colostrum or the like by a suitable purification method , such as affinity chromatography using carbohydrate - derivatised matrices , such as mannose or mannan coupled matrices . such a method is discussed in wo99 / 64453 , wherein the purification process is followed by a virus - removal step in order to remove infectious agents from the mbl source , since one of the major problems with proteins purified from body liquids is the risk of introducing infectious agents in combination with the desired protein . wo99 / 64453 is hereby incorporated by reference . the mbl composition used to manufacture an mbl medicament preferably comprises mbl oligomers having a size distribution substantially identical to the size distribution of mbl in serum , such as a size distribution profile at least 50 % identical to the size distribution profile of mbl in serum . by identical is meant that at least 50 % of the oligomers has an apparent molecular weight higher than 200 kda , when analysed by sds - page and / or western blot . in a more preferred embodiment the size distribution profile is at least 75 % identical to the size distribution profile of mbl in serum , such as at least 90 % identical to the size distribution profile of mbl in serum , and more preferred at least 95 % identical to the size distribution profile of mbl in serum . when purifying from an mbl source initially having another size distribution profile it is preferred that the affinity chromatography used to purify from the mbl source favours purification of oligomers having an apparent molecular weight higher than 200 kda . this is obtained by using a carbohydrate - derivatized matrix having substantially no affinity to subunits and / or dimers of mbl . preferably the carbohydrate - derivatized matrix has affinity for substantially only tetrameric , pentameric and / or hexameric recombinant mbls . the matrix may be derivatized with any carbohydrate or carbohydrate mixture whereto mbl binds and for which binding of the higher oligomers of mbl are favoured . the carbohydrate - derivatized matrix is preferably a hexose - derivatized matrix , such as a mannose - or a n - acetyl - glucosamin derivatized matrix , such as most preferably a mannose - derivatized matrix . the selectivity of the carbohydrate - derivatized matrix is obtained by securing that the matrix as such , i . e the un - derivatized matrix has substantially no affinity to mbl polypeptides , in particular no affinity to mbl trimers or smaller oligomers . this may be ensured when the matrix as such is carbohydrate - free . in particular the matrix should not contain any sepharose or the like . it is preferred that the matrix consists of a non - carbohydrate containing polymer material , such as fractogel ® tsk beads . the matrix may be in any form suitable for the chromatography , mostly in the form of beads , such as plastic beads . after application of the mbl source the column is washed , preferably by using non - denaturing buffers , having a composition , ph and ionic strength resulting in elimination of proteins , without eluting the higher oligomers of mbl . such as buffer may be tbs . elution of mbl is performed with a selective desorbing agent , capable of efficient elution of highed oligomers of mbl , such as tbs comprising a desorbing agent , such as edta ( for example 5 mm edta ) or mannose ( for example 50 mm mannose ), and mbl oligomers are collected . such a purification method is described in co - pending international patent application no . wo 00 / 70043 . in a preferred aspect a clinical grade mbl composition is obtained by using an mbl source produced by recombinant technology , wherein the mbl source is the culture media from culturing of mbl producing cells . thus , the present invention encompasses mbl produced by a process of producing a recombinant mannan binding lectin ( mbl ), comprising the steps of : preparing a gene expression construct comprising a dna sequence encoding a mbl polypeptide or a functional equivalent thereof , transforming a host cell culture with the construct , cultivating the host cell culture , thereby obtaining expression and secretion of the polypeptide into the culture medium , followed by obtaining a culture medium comprising human recombinant mbls . the culture medium comprising the human recombinant mbl polypeptides may then be processed as described above for purification of mbl . the mbl polypeptide is preferably a mammalian mbl polypeptide , such as more preferably a human mbl polypeptide . the gene expression construct may be produced by conventional methods known to the skilled person , such as described in u . s . pat . no . 5 , 270 , 199 . in another embodiment the gene expression construct is prepared as described in wo 00 / 70043 . the expression is preferably carried out in e . g . mammalian cells , the preparation according to the invention results from the use of an expression vector comprising intron sequence ( s ) from an mbl gene and at least one exon sequence . regarding the transgenic animals as expression system this term is in this context animals which have been genetically modified to contain and express the human mbl gene or fragments or mimics hereof . in addition to the purification method it is preferred that the gene expression construct and the host cell also favours production of higher oligomers , which has been found to be possible by using a gene expression construct comprising at least one intron sequence from the human mbl gene or a functional equivalent thereof . malian cells and cells from insects . consequently , the mbl composition may be used for preventing and / or treating sars in an individual wherein the microbial species is a fungus , a yeast , a protozoa , a parasite and / or a bacteria . the medicament may be produced by using the eluant obtained from the affinity chromatography as such . it is however preferred that the eluant is subjected to further purification steps before being used . in addition to the mbl oligomers , the medicament may comprise a pharmaceutically acceptable carrier substance and / or vehicles . in particular , a stabilising agent may be added to stabilise the mbl proteins . the stabilising agent may be a sugar alcohol , saccharides , proteins and / or amino acids . examples of stabilising agents may be maltose or albumin . other conventional additives may be added to the medicament depending on administration form for example . in one embodiment the medicament is in a form suitable for injections . conventional carrier substances , such as isotonic saline , may be used . in another embodiment the medicament is in a form suitable for pulmonal administration , such as in the form of a powder for inhalation or creme or fluid for topical application . the route of administration may be any suitable route , such as intravenously , intramusculary , subcutanously or intradermally . also , pulmonal or topical administration is envisaged by the present invention . normally from 1 - 100 mg is administered per dosage , such as from 2 - 10 mg , mostly from 5 - 10 mg per dosage depending on the individual to be treated , for example about 0 . 1 mg / kg body weight is administered . the use of an mbl composition for the manufacture of a medicament may also further comprise the manufacture of another medicament , such as an anti - fungal , anti - yeast , anti - bacterial and / or anti - viral medicament for obtaining a kit - of - parts . the anti - viral medicament may be a medicament capable of virus attenuation and / or elimination . the invention also relates to an aspect of using a measurement of the mbl level as a prognostic marker for the risk of the individual of acquiring sars and thereby an indicative of the need for treatment . in particular an mbl level below 500 ng / ml is a prognostic marker indicative for treatment with mbl . thus , the present invention also relates to a method of using an mbl composition for preventing and / or treating sars in an individual , the method comprising the steps of : i ) determining serum levels of mbl in an individual , ii ) estimating the probability of the occurrence of a significant clinical sars in the individual , and optionally , the mbl level is measured in serum or plasma , and may be determined by time resolved immunofluorescent assay ( trifma ), elisa , ria or nephelometry . also the mbl levels may be inferred from analysis of genotypes of the mbl genes as discussed above in relation to mutations of mbl leading to a decreased mbl level . patients are selected among individuals presenting clinically significant sars as defined above . patients are identified by retrospective computer search of the patient database . before entering treatment blood is drawn into evacuated glass tubes containing edta ( final concentration about 10 mm ). the plasma is aliquoted and kept at − 80 ° c . until assay . plasma samples are similarly obtained from healthy blood donors . the patients are free of infections at the time of blood sampling . the concentration of mbl is determined by a time resolved immunofluorescent assay ( trifma ). microtitre wells ( fluoronunc , nunc , kamstrup , denmark ) are coated with antibody by incubation overnight at room temperature with 500 ng anti - human mbl antibody ( mab 131 - 1 , statens serum institut , copenhagen , denmark ) in 100 μl pbs ( 0 . 14 m nacl , 10 mm phosphate , ph 7 . 4 ). after wash with tween - containing buffer ( tbs , 0 . 14 m nacl , 10 mm tris / hcl , 7 . 5 mm nan 3 , ph 7 . 4 with 0 . 05 % tween 20 ) test samples ( plasma 1 / 20 ) and calibrator dilutions are added in tbs / tween with extra nacl to 0 . 5 m and 10 mm edta . after overnight incubation at 4 ° c . and wash , the developing europium - labelled antibody ( 12 . 5 ng mab 131 - 1 labelled with the eu - containing chelate , isothiocyanato - benzoyl - diethylene - triamine - tetra acetic acid , according to the manufacturer , wallac , turku , finland ) is added in tbs / tween with 25 μm edta . following incubation for 2 h and wash , fluorescence enhancement solution is added ( wallac ) and the plates are read on a time resolved fluorometre ( delfia 1232 , wallac ). the calibration curve is made using dilutions of one plasma , which is kept aliquoted at − 80 ° c . based on the above outlined method , the mbl serum level of patients with sars as compared to non - sars patients is compared . prototype sars - cov hku39849 were grown on the fetal rhesus kidney cells ( frhk - 4 ) ( atcc ). the cells were grown in mem medium with 10 % fetal calf serum . virus titers ( tcid 50 ) were determined by titration of a 10 - fold dilution series on frhk - 4 cells . binding of mbl to sars - cov strains was tested with an elisa in which suspensions of virus ( 10 3 to 10 5 tcid 50 ) diluted in pbs were incubated overnight at 4 c on 96 - well plates , followed by washing and incubation with mbl . before the addition of mbl , plates were blocked with bsa and gelatin in pbs and washed with hbss + 0 . 05 % tween 20 . mbl ( 0 to 10 μg / ml ) diluted in ca 2 + containing hbss were then added and allowed to incubate with virus for 2 hours , followed by the washing off of unbound lectin ( three times in hbss ). control experiments were done by addition of edta or mannan to some of the mbl containing wells . the presence of bound mbl was detected with biotinylated monoclonal anti - 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