Patent Application: US-201214126673-A

Abstract:
the invention relates to methods and apparatus for determining properties of a surface . embodiments disclosed include an apparatus for measuring a surface charge of a sample , comprising : a sample holder having an opposed pair of electrodes and configured to hold a sample in position in a measurement volume between the electrodes such that a planar surface of the sample is aligned orthogonal to the electrode surfaces ; a measurement chamber for containing a measurement liquid and having an open end configured to receive the sample holder to position the electrodes in a preset orientation ; a laser light source positioned and configured to direct a laser beam through the measurement chamber between the electrodes and parallel to the planar surface of the sample when the sample holder is received in the measurement chamber ; and a detector positioned and configured to detect scattered light from the measurement volume , wherein the apparatus is configured to allow for detection of the scattered light by the detector over a range of distances from the surface of the sample .

Description:
aspects and embodiments of the invention are described in further detail below by way of example and with reference to the enclosed drawings in which : fig1 is a perspective schematic diagram of an illustrative zeta potential measurement instrument according to the invention ; fig2 is a schematic diagram illustrating flow geometry for a surface under test ; fig3 is an elevation view diagram of a sample holder test cell for the instrument of fig2 ; fig4 is a cross - sectional diagram of a sample holder test cell head for the instrument of fig2 showing different measurement beam positions for different test cell head positions ; fig5 is a perspective drawing of an implementation of the sample holder test cell of fig3 ; fig6 is a plan view diagram of the sample holder test cell of fig3 in position in an adjustment or calibration jig ; fig7 a to 7 c are side elevation views of an exemplary sample holder test cell configured for two distinct orientations relative to a measurement chamber ; fig8 a is a plot of voltage applied to the test cell of fig3 as a function of time ; fig8 b is a plot of voltage applied to the test cell along with current passing through the test cell as a function of time ; fig9 is a schematic diagram of the test cell electrode assembly of fig4 illustrating particle and fluid flow resulting from voltage applied to the cell at different measurement positions ; fig1 a is a family of plots of displacement ( as indicated by phase ) as a function of time for the measurement positions a to e illustrated in fig9 ; fig1 b is a further family of plots of displacement as a function of time for an increased number of measurement positions ; fig1 is a plot of reported potential as a function of displacement from the sample surface for the measurement positions illustrated in fig9 ; fig1 is a plot of r 2 as a function of displacement for water at various temperatures ; fig1 is a plot of surface potential for a series of measurements for a ptfe sample ; fig1 is a plot of surface charge as a function of ph for a ptfe sample measured using the instrument of fig1 and with other techniques ; fig1 is a plot of surface potential as a function of ph for a sample of silica measured using the instrument of fig1 and with other techniques ; fig1 is a plot of surface potential normalized to a value recorded at 25 ° c . as a function of temperature for the instrument of fig1 and with other techniques ; fig1 is a plot of surface potential as a function of pc for a ptfe sample measured using the instrument of fig1 and using other techniques ; fig1 is a plot of surface potential as a function of pc for a polycarbonate sample measured using the instrument of fig1 and using other techniques ; fig1 a is a plot of apparent zeta potential as a function of displacement for a sample of clean ptfe in goethite at ph 3 . 5 ; fig1 b is a plot of apparent zeta potential as a function of displacement for a sample of ptfe with dried - on goethite in goethite at ph 3 . 5 ; fig1 c is a plot of apparent zeta potential as a function of displacement for a sample of peek 450g in dts0230 at ph 9 . 0 ; fig1 d is a plot of apparent zeta potential as a function of displacement for a sample of ptfe in dts0230 at ph 9 . 0 ; and fig2 a to 20 e are plots of normalised particle displacement measurements over time intervals varying between 75 ms ( fig2 a ) and 600 ms ( fig2 e ) as a function of displacement from the surface of a smooth flat ptfe block in 1 mm kcl at ph 9 . 0 and ph 2 . 67 . referring to fig1 , an exemplary surface charge measurement instrument 10 according to an aspect of the invention includes a light source 14 , a sample cell 16 for holding a sample 22 under test with its test surface 12 in contact with an electrolyte , and a detector 18 . the instrument 10 may be used to determine the zeta potential of the test surface 12 of the sample 22 under test . although other configurations are possible , the light source 14 and the detector 18 preferably form part of a particle measurement system along with other optical elements that enable the system to perform a laser doppler phase electrophoretic analysis light scattering measurement protocol , such as is described in reference [ 12 ]. particle measurement instruments of this type are available from malvern instruments ltd of malvern , uk , examples of which are described further in international patent application pct / gb2009 / 051350 , published as wo / 2010 / 041082 , which is herein incorporated by reference . referring also to fig2 , the instrument 10 operates according to an optical technique that measures the electro - osmotic flow of an electrolyte near a single charged test surface 12 with an external field applied parallel to the surface . the technique uses a single test plate presented to the optical detection system in a convenient ‘ dip cell ’ format that can fit inside a standard type of cuvette and can therefore be used in instrumentation intended for electrophoresis measurements , such as the zetasizer range of instruments from malvern instruments ltd . a surface 12 under test is immersed in an electrolyte 96 with an external electric field e x applied . the technique is characterized by displacements of the order of 100 &# 39 ; s of micrometres ( μm ) and the slipping plane of the surface 12 under test can then be assumed to coincide with the plane of y = 0 . the electric field e x and the presence of the ionic species within the electrolyte cause electro - osmotic fluid motion along the surface at y = 0 . assuming that the system has no pressure gradients , is slow flowing and in a steady state , the navier - stokes equation reduces to where v ( t , x , y ) is the component of fluid velocity parallel to the boundary , ρ is the fluid density and η is the fluid viscosity . the co - ordinate x is parallel to the boundary , and y is perpendicular . because there is no flow perpendicular to the boundary , continuity implies that v is not a function of x , and the equation simplifies to the following one dimensional homogenous heat or diffusion equation : where k = η / ρ . this , with the initial condition that v ( 0 , y )= 0 and boundary condition v ( t , 0 )= v eo where v eo is the fluid velocity at the boundary we have a problem on the half line ( 0 , ∞) with homogenous initial conditions and dirichlet boundary conditions and has a green function solution that can be expressed in closed form as follows : where erf is the error function . for water at 25 ° c . the term in square brackets in equation ( 4 ) disappears at y ≧ 750 μm for t ≧ 75 ms or y ≧ 1 . 5 mm for t ≧ 300 ms . time intervals of this magnitude are typical for monitoring electrophoretic motion using pals and adjustment of the surface under test with respect to the detection optics is easily achieved within these distances with a micrometer stage . therefore , a fit of equation ( 4 ) to measurements of v i ( y i ) at various points y i can then be extrapolated to the y - axis intercept to yield v eo . this is described in more detail in section 2 . 2 . 2 . 1 of reference [ 35 ]. the relationship between the surface zeta potential ζ and v eo is given by : where e x is the electric field strength , ε the electrolyte relative permittivity and η the electrolyte viscosity ( see also reference [ 11 ]). the fluid motion measurement ( at points y i ) is achieved by detection of the movement of tracer particles dispersed in the electrolyte alongside the surface under test , with the mobility being measured using a pals ( phase analysis light scattering ) technique . referring also to fig3 to 5 , the sample holder test cell 16 includes a first portion 34 and a second portion in the form moveable section 36 having a test cell head 20 supported by , and located at a distal end . the test cell head 20 , further detailed in fig4 , includes a pair of electrodes 24 , 26 , which are preferably plate - shaped , i . e . planar . the electrodes 24 , 26 are positioned either side of a measurement volume 27 in which a test sample 22 can be located with a planar test surface 12 oriented orthogonally to the planar surfaces of the electrodes 24 , 26 . in use , the test cell head 20 comprising the electrodes 24 , 26 and test sample 22 is immersed in an electrolyte that includes tracer particles , the electrolyte providing a medium across which an electric field is applied by the electrodes 24 , 26 . the sample holder 30 includes an adjustment mechanism 32 , such as a micrometer , which is supported by a static section 34 . the adjustment mechanism 32 allows the position of the test cell head 20 to be adjusted relative to the static section 34 in a direction normal to the test surface 12 so that a measurement beam 28 ( fig4 ) from the light source 14 is directed across the test surface 12 over a range of distances from the test surface 12 . the supporting section 32 of the test cell body 30 may include one or more interface surfaces 38 that interface mechanically and electrically with portions of the instrument to allow the test cell to be removably and precisely positioned in the instrument with respect to the measurement beam 28 . in the exemplary embodiment illustrated in fig5 , the test cell 16 is designed to conform to a standard form factor for test cuvettes for the above mentioned zetasizer instrument line . the adjustment mechanism 32 may be manually operated or may be motorized . the test cell 16 may be mounted in a calibration jig 40 , as shown in fig6 . the adjustment mechanism 32 can then be used to adjust the position of the surface of the test sample 22 until there is no gap between the surface and a corresponding mating surface of the calibration jig 40 ( indicated at position a in fig6 ). a user can then set the adjustment mechanism 32 , for example in the form of a micrometer , to a required measurement position from this datum . this technique allows for different test plate thicknesses to be accommodated . the test cell 16 can then be removed from the jig and immersed in the measurement chamber , or cuvette , containing tracer particles dispersed in the target liquid electrolyte , and placed in the instrument for measurement . in general , the data obtained using the test cell of the type described herein tend to be highly reproducible , with standard engineering tolerances being sufficient to reduce uncertainties to a minimum . for increased reproducibility , an additional feature may be incorporated in the test cell , an example of which is illustrated in fig7 . in this embodiment , a micrometer adjustment mechanism is used to set the correct height of the test surface with respect to the beam to accommodate the thickness of the test sample , for example using a calibration jig as described above . once calibrated , the test cell may be positioned in two or more preset measurement positions relative to the measurement chamber , each position having a preset distance between the measurement beam and the test surface of the sample . this may be achieved by providing a stepped region 38 on the static or supporting section 34 of the test cell , the stepped region 38 allowing for two different positions depending on the orientation of the test cell relative to the measurement chamber . in the exemplary embodiment illustrated in fig7 , the test cell may be rotated through 180 ° ( π radians ) to select which relative position is chosen . if the linear assumption according to the relationship in equation 2 is correct , two measurement positions should be sufficient to obtain a value for both m and v eo . for increased accuracy , however , further preset positions may be preferred , which may be achieved for example by providing a series of two or more stepped regions allowing for a corresponding plurality of different measurement positions . in one exemplary embodiment , the test cell may have a 500 μm pitch thread and the cell position y , i . e . the distance between the static section 34 and the end of the moveable portion 36 , may be adjusted by winding an adjustment knob against a biasing force provided by a spring , thereby reducing hysteresis and relative positional uncertainty to low or negligible levels . in order to set a zero point for a plate of arbitrary thickness , the cell can be adjusted downwards relative to the measurement chamber until the laser beam is on the point of being obscured , as determined by monitoring a count rate in the forward angle . in this exemplary embodiment , the laser beam passes through the cell as shown in fig1 with a beam width or around 40 μm , resulting in a maximum uncertainty of around ± 20 μm . particle mobility data may also be recorded and the results can be reduced using the relationship of equation 4 . in operation , referring also to fig9 , charged tracer particles 95 suspended in the liquid electrolyte 96 move under the application of the electric field between the opposing electrodes 24 , 26 . the tracer particles 95 scatter light from the illuminating beam , which is detected by the detector 18 . by use of a reference beam 29 ( fig1 ) split off from the incident beam 15 prior to illuminating the sample , the phase of the scattered light 17 relative to the incident light 15 can be measured . this phase is linearly related to the speed of the tracer particles 95 in the measurement liquid 96 . since the static or supporting section 34 of the test cell 30 is fixed relative to the illuminating optical measurement beam 28 ( fig4 ), the plate position can be altered by means of the adjustment mechanism 32 ( fig5 ), such as a micrometer , thereby translating the moveable section 36 ( fig3 ) relative to the supporting section 34 and therefore with respect to the measurement beam 28 . multiple measurements can thereby be taken for a sample with the measurement beam 28 at multiple distances from the test sample surface 12 . as illustrated schematically in fig9 , on the application of an electric field across the measurement volume between the electrodes 24 , 26 , the tracer particles 95 move under the influence of both the electro - osmotic motion of the fluid , v i ( y i ) ( indicated by solid vectors 91 a - d ) and due to electrophoretic motion , v ep ( indicated by dashed vectors 92 a - e ). the total detected motion is given by the sum of these vectors , which in fig9 is indicated by dotted vectors 93 a - e . the field strength , e x , can be determined from a measurement of the conductivity of the measurement liquid and a measurement of electric current during application of the field . this tends to yield a more accurate estimate of e x than by calculating the field from the potential applied to the cell . fig8 a illustrates a plot of voltage applied to the electrodes of an exemplary test cell over time . in this case , the voltage is switched at a frequency of around 0 . 42 hz , which is done to minimize any polarization concentration effects due to charge migration , which can cause increased uncertainty in the current estimate during the field application . an off - time 50 of around 200 ms between successive applications of opposing voltages over 600 ms periods is used in order to allow the system to relax to zero before a subsequent reverse polarity is started . fig8 b is a further plot of voltage 81 ( left hand scale ) and current 82 ( right hand scale ) as a function of time for an exemplary test cell . the voltage 81 is applied in the form of a series of square wave pulses , and the resulting current 82 peaks after the rising and falling edges of each pulse , followed by a gradual decay . fig1 a illustrates a series of plots of phase ( corresponding to particle displacement ) as a function of time . the displacement per unit time ( i . e . particle velocity ) falls to around zero during the interval between successive voltage pulses . the overall displacement during each on - time pulse is taken as the average over the whole of the on - time period . this is based on a simplified model that ignores effects such as those due to inertia or pressure gradients but , as the results in the following sections show , this simple model provides measurements that are reasonably linear , in accordance with the relationship in equation 2 , and which yield results that are precise and accurate in comparison to literature values based on other techniques . fig1 b illustrates a similar series of plots of phase as a function of time , for an increased number of beam positions relative to the sample surface . different measurement strategies can be employed to obtain a measure of surface charge of a sample . a set of measurements may be taken using a slowly varying field , as described above , at multiple beam positions , the positions set using adjustment of a micrometer . the micrometer may be motorised or manually adjusted . slowly reversing field measurements may alternatively be taken at two positions only , for example by reversing the orientation of a suitably configured sample holder , as illustrated in fig7 and described above . this may lead to increased uncertainty due to an extrapolation based only on two data points , but has an advantage of simplifying operation and improving repeatability . alternative types of measurements can be performed where one or two positions are used for the measurement . an appropriate model is fitted to the slow field phase plot and the electro - osmosis and electrophoresis calculated from the model . the electrophoresis may be measured either at the same position as the slow field or at the second positions , further away from the wall . this measurement strategy allows the determination of the sign of the wall charge , and is discussed in more detail in u . s . pat . no . 7 , 217 , 350 and ep 1154266 , which are both herein incorporated by reference . the reported zeta potential values from measurements taken on a sample consisting of a ptfe block immersed in a ph 9 . 2 buffer and using carboxylated latex tracer particles are shown in fig1 , as plotted against the displacement from the surface . as the optical detection position moves away from the surface ( points a to d ), the electro - osmotic contribution to the resulting motion reduces in value until , at some position e , the detected motion is in effect only the electrophoretic motion of the tracer from which the tracer zeta potential ζ ep can be calculated ( corresponding to the region between positions d and e in fig1 ). the y - axis intercept can be extrapolated from a linear fit to the data over points a to d and the surface zeta potential at the slipping plane , i . e . where y = 0 , is then given by the following : the data were reduced using a least squares linear regression of the potentials reported at each displacement against the displacement from the surface . the standard error in the intercept was then added in quadrature to the uncertainty in the electrophoretic mobility ( recorded at position e ) in order to give a measure of overall uncertainty in the surface zeta potential . a linear fit avoids the region beyond point d whilst , conversely , extending as far out as possible from the surface , in order to provide a more accurate estimate of the slope and thereby the intercept . the viscosity of the dispersant in which the cell is immersed will change with temperature . specifically , less viscous fluids will couple less efficiently with increasing distance from the sample surface and therefore we would expect higher temperatures to exhibit a lower electro - osmotic component at the same distance than at lower temperatures . to assess this , a silica plate was measured in 1 mm kcl at ph7 . 0 +/− 0 . 1 using a milk substitute as the tracer . the results are presented in the form of r 2 values of the mean values of reported potential at displacements of up to y = 750 μm and for a range of temperatures , as plotted in fig1 . at 25 ° c . the fit is good ( indicated by an r 2 value close to 1 ) out to greater than 750 μm but , as expected , as the temperature increases the linear region reduces in extent with even a 5 ° c . increase in temperature enough to reduce the r 2 value from & gt ; 0 . 99 to approximately 0 . 97 at 750 μm . as a result , measurements are preferably conducted at or around ambient temperature , nominally 25 ° c ., and with a maximum displacement set by the point at which the r 2 value falls below 0 . 99 . various measurements were performed to demonstrate the accuracy , precision and reproducibility of the new technique using a comparison with measurements by other techniques reported in the literature . reproducibility of the technique was investigated for a known well behaved system of a ptfe block and 300 nm carboxylated latex beads dispersed in ph9 . 2 buffer . latex is known to have a stable zeta potential of − 68 mv +/− 10 % at this ph , which can be measured using laser doppler electrophoresis ( lde ) for extended periods without degradation . measured surface potentials of the ptfe sample are shown in fig1 . each experiment was conducted with a new tracer dispersion and after having cleaned the ptfe block and electrodes with helmanex ® followed by copious amounts of deionised water and a brush . no outliers were removed from the data in fig1 and the overall surface zeta potential result of − 70 . 0 mv +/− 7 . 5 mv is in excellent agreement with the mean value from the available literature values indicated in table 1 above of − 67 . 0 mv +/− 11 mv . the goethite , nist traceable standard for dispersed zeta potential measurements quotes a pass / fail rsd of 10 %, indicating that the technique is capable of reproducing surface potential measurements to approximately the same uncertainty , given by —( 7 . 5 / 70 )× 100 %= 11 %. titrations of surface potential against ph are likely to be one of the primary applications for this technique . a series of measurements of ptfe and silica were conducted in 1 mm kcl and the ph varied using hcl and koh . a milk substitute ( described in further detail in reference [ 35 ]) was used as a tracer for all measurements . each ph point corresponds to a separate measurement sequence using the cell . fig1 and 15 show that the results are in good general agreement with streaming potential , dispersed particles and capillary electro - osmosis measurements at all ph values tested in the region of the isoelectric point ( iep ). there is less general agreement at higher ph values but this appears to be a general feature of all techniques . for data obtained by the new technique , the surface potential would be expected to saturate at high ( and low ) ph as all available surface charge groups are ionised . looking at the error bars in both plots , no trend between the uncertainty and ph is seen to exist , with a typical uncertainty of +/− 2 to 3 mv . the isoelectric point for the milk substitute is at ph4 . no increase in uncertainty in the reported surface potential is detected either when the surface iep is at approximately the same value as the surface ( fig1 ) or at a different value ( fig1 ). this indicates that the iep of the tracer may be ignored as long as the experiment does not extend for long enough for it to stick to the surface under test . in this case , each displacement took 120 s to record , but a single dispersion was used for each ph point over five or six displacement positions so the samples were immersed for up to 20 minutes without any apparent reduction in accuracy . kirby & amp ; hasselbrink ( reference [ 22 ]) note that the scientific record is sparsely populated with studies concerned with the relationship between surface potential and temperature . a small number of notable contributions exist which strongly disagree with each other . as a comparison , the surface potential of a silica test plate was measured as an application specific demonstration of the performance of the technique with temperature . much of the experimental evidence for the relationship between zeta potential and temperature is due to ishido & amp ; mizutani ( reference [ 2 ]) and somasundaran & amp ; kulkani ( reference [ 26 ]), whose streaming potential measurements predict an increase of 1 . 75 % per ° c . in zeta potential of silica in 1 mm and 10 mm kno 3 at ph7 . 0 . an uncoated microscope slide was cut and mounted in the cell and the surface zeta potential measured in 1 mm kcl at ph 7 . 0 +/− 0 . 1 . the data are shown in fig1 with a plot of surface potential , normalized to the value recorded at 25 ° c ., against temperature . our data predict a slope of 0 . 34 %, which is in poor agreement with the streaming potential results but is in excellent agreement with a more recent study using capillary electrophoresis by evenhuis et al ( reference [ 5 ]), who measured a slope of 0 . 39 % per ° c . the error bars demonstrate that the technique is reproducible with temperature variation with an rsd varying from 5 % to 10 % and that no apparent increase in uncertainty exists with temperature . the whole cell ( dip cell , cuvette and dispersant ) is immersed in the instrument &# 39 ; s temperature controlled cell block and setting each temperature is therefore simpler to implement in comparison to other techniques as the whole apparatus can be kept at the same set temperature ( see reference [ 22 ]). increasing salt concentration increases the current passed for the same field strength , which can cause joule heating and polarization concentration effects . these can increase the uncertainty in the recorded particle mobility . surface potential measurements of polycarbonate and ptfe test blocks in kcl were carried out between 0 . 1 mm and 50 mm salt concentration with a milk substitute used as the tracer . in order to avoid joule heating the conductivity of the sample was measured before and after the electrophoresis measurement and the field strength titrated down until the difference in conductivity and therefore sample temperature was negligible . table 2 below shows the field strengths and subsequent integration times used to maximize the signal to noise at each concentration c , where , pc =− logc . the data for ptfe are shown in fig1 and for polycarbonate in fig1 . firstly , we note that with the field strengths and integration times used in table 2 , no relationship between the uncertainty in the measurements and pc is apparent . the uncertainties in the ptfe data are of the order of the spread in the mean results from all techniques and significantly less so for the polycarbonate case . we would expect a linear relationship between surface potential and pc passing through the origin between surface potential and salt concentration for monovalent counter ions . the polycarbonate dip cell data are self consistent in that they fit linearly with near zero intercept but there is considerable variation amongst the references . since both the dip cell and the roberts et al data ( reference [ 23 ]) are linear with low intercept then we can attribute the difference to a genuine difference in sample properties such as surface smoothness , for instance — in our case , the polycarbonate was a small block removed from a moulded part with a highly polished surface . the ptfe results are in good overall agreement with the literature values although an overall intercept of zero is less convincing in these data , with a more likely intercept nearer to + 20 mv . fig1 a to 19 d illustrate further results of reported zeta potential for various combinations of samples and electrolytes . fig1 a indicates results from a clean ptfe plate in a goethite solution at ph 3 . 5 . fig1 b indicates results from a ptfe plate having dried - on goethite in a goethite solution at ph 3 . 5 . the differences between these sets of measurements indicate the importance of ensuring a clean sample to obtain accurate measurements of the sample material uncontaminated by suspended or dissolved material in the electrolyte . fig1 c indicates results from a peek 450g plate in a dts023 solution at ph 9 . 0 , and fig1 d indicates results from a ptfe plate in the same type of solution . the results from these measurements are summarised below in table 3 , with selected results compared with those from reference [ 1 ]. fig2 a to 20 e illustrate plots of normalised particle displacement measurements for time intervals between 0 and 75 ms ( fig2 a ), 0 and 150 ms ( fig2 b ), 0 and 300 ms ( fig2 c ), 0 and 450 ms ( fig2 d ) and 0 and 600 ms ( fig2 e ) for a smooth , flat ptfe block mounted and measured in 1 mm kcl at ph 9 . 0 and ph 2 . 67 . at ph 9 . 0 the surface is highly negatively charged and at ph 2 . 67 , highly positively charged ( see reference [ 1 ]). the milk substitute used for the tracer particles was also negative at ph 9 . 0 and positive at ph 2 . 67 ( see further details in appendix b of reference [ 35 ]). each field half cycle was 600 ms in total duration . a least square linear fit was applied to the phase plot ( fig1 a ) over various subsets of 75 ms , 150 ms , 300 ms , 450 ms and 600 ms of this interval to yield an average phase shift per unit time at each displacement point a - e . the total particle displacement in the time interval in question , at each distance from the test surface , is directly proportional to the average total phase shift during the ontime pulse ( fig8 a ). this total particle displacement is plotted in each of fig2 a to 20 e . the experimental data were also normalised between a 2nd order polynomial extrapolation of the data to the intercept ( y = 0 ) and from an estimate of the asymptote towards point e indicated in fig9 . this asymptote is due to the limiting electrophoretic mobility of the tracer particles in the absence of any electro - osmotic flow far from the surface . normalisation removes the tracer velocity and allows direct comparison of the data with the model indicated by equation ( 4 ) above . the fit to the model is excellent for the phase data at and below 300 ms for both positively and negatively charged surfaces . above 300 ms the fit is less convincing and we attribute this to a gradual build up in back pressure which works to suppress the electro - osmotic flow . further explanation of these results , and the associated model , is disclosed in reference [ 35 ], the contents of which are incorporated by reference herein . in conclusion , a new , simpler technique for the measurement of surface zeta potential using laser doppler electrophoresis has been presented . the technique is shown to be characterized by a relative standard deviation in reproducibility of less than or equal to around 10 % for well behaved systems , yielding accurate and reproducible surface potential values in excellent agreement with literature values from streaming potential , electro - osmotic ( capillary ) flow and particle dispersions for various surface types , temperatures up to 40 ° c . and ionic strengths in the range 0 . 1 mm to 50 mm . the present invention has now been described in connection with a number of specific embodiments thereof . however , numerous modifications which are contemplated as falling within the scope of the present invention should now be apparent to those skilled in the art . for example , while micrometer - and joggle - based approaches have been shown to adjust the detection position , other approaches such as moving mirrors could also be employed . therefore , it is intended that the scope of the present invention be limited only by the scope of the claims appended hereto . in addition , the order of presentation of the claims should not be construed to limit the scope of any particular term in the claims . all documents referenced in this application are herein incorporated by reference for all purposes . 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