Patent Application: US-27517503-A

Abstract:
this invention relates to a method for treating a tendon or ligament disorder , comprising the step of administration of a pharmaceutical or veterinary composition of igf to the affected area of a subject in need of such treatment . the tendon or ligament disorder may be the result of a traumatic , exercise - related or overuse injury , or may be the result of , or associated with , a pathological condition . in preferred embodiments the traumatic or exercise - related injury is a ruptured tendon or ligament ; a severed tendon or ligament ; a tendon or ligament avulsion ; a tendon or ligament sprain ; limping or lameness ; or the pathological condition is a disease , such as an inherited disease , an endocrinological or metabolic condition , an infectious disease , or a disease attributed to inflammation of the tendon , ligament or surrounding tissue . the invention further relates to compositions for the treatment of these conditions .

Description:
the invention will now be described in detail by way of reference only to the following non - limiting examples . suitable formulations for use in intralesional or localised applications of igf in accordance with this invention , and methods of preparation thereof , include the following , in which “ igf ” may be igf - i , igf - ii or an analogue thereof : igf quantity specified ( qs ), dissolved in 100 mm acetic acid and diluted in phosphate buffered saline stabilized with aspartate , and stored at 4 ° c . until required . fibrinogen solution : 0 . 04 - 0 . 20 g added to 10 mls of 0 . 9 % ( w / v ) sodium chloride which has been sterilised by membrane filtration ( 0 . 22 μm membrane ) and kept warm at 37 ° c . kept at 37 ° c . until required . igf qs is added to the fibrinogen solution prior to gel formation . to make gel , add thrombin to fibrinogen at a ratio of 1 : 10 . igf qs is added to the collagen solution prior to gel formation . igf qs is added to the methylcellulose solution prior to gel formation . intralesional injection of igf - i promotes intrinsic repair of acute collagenase - induced tendinitis in horses studies on the pathogenesis of equine tendinitis following collagenase injury concluded that this particular model can justifiably be used as a model of traumatic and overuse injuries ( williams et al ., 1984 ) 12 healthy standardbred horses with collagenase - induced tendinitis in their nearside forelimbs were used in this study . tendinitis was induced in the superficial digital flexor tendon ( sdft ), and the contralateral undamaged tendon was used as a control , with the undamaged deep digital flexor tendon ( ddft ) as an additional control . treatments involved administration by intralesional injection of either active drug ( 250 μg of igf - i formulated in 100 μl of phosphate - buffered saline ) to six ( 6 ) horses or placebo ( 100 μl of vehicle ) to the other six ( 6 ) horses on two occasions seven days apart , and monitoring the progress of tissue repair over the ensuing 8 weeks by ultrasound image analysis of samples of “ core lesions ”. the study investigators were blinded to the treatments . the digitised images were used to analyse the echoic properties of the “ core lesion ” using a mean grey scale . this analysis correlates the proportion of reflected soundwaves ( the whiteness of ultrasound images ) to the proportion of connective tissue present within the “ core lesion ” during the healing process . therefore mean grey scale change over time can be used as a measure of wound healing by expressing the mean cross - sectional soundwave echo of “ core lesions ” as degrees of whiteness . for example , the whiter the mean cross - sectional area , the more connective tissue is present , and the greater is the progression toward repaired tendon . table 1 shows the mean gradients and standard error of the mean ( sem ) gradients for the two treatment groups , and the statistical significance ( p ) of igf - i treatment , as determined from repair rates generated by image analysis of core lesions . the means were calculated from individual values of the mean grey scale of the treated core lesion within the superficial digital flexor tendon ( sdft ), expressed as a percentage of the undamaged deep digital flexor tendon ( ddft ), normalised to the contralateral undamaged tendon . linear regression curves generated from the mean grey scale data analysis illustrated in table 1 demonstrate significantly steeper gradients for horses treated with igf - i compared to control horses . the proportion of type i and type iii collagen in injured tendon was also determined . tissue from core lesions was excised 15 weeks after tendinitis was induced , dried , frozen and ground to a fine powder under liquid nitrogen in a spex machine . ground tissue was dissolved in 10 × volume ( v / w ) 0 . 5m acetic acid containing complete ™ protease inhibitors ( roche ) and digested with pepsin ( 10 : 1 tissue : pepsin w / w ) overnight at 4 ° c . with constant mixing . solutions were clarified by centrifugation ( 45 minutes at 30 , 000 g ) and the supernatant transferred to new vials . ice - cold 4m nacl was added to the supernatant to a final concentration of 2m nacl , mixed by inversion and incubated on a rotary mixer for 30 minutes at 4 ° c . collagenous proteins were pelleted by centrifugation ( 45 minutes at 30 , 000 g ) and the supernatant poured off . the collagenous proteins were redissolved in 10 ml of 0 . 5m acetic acid and dialysed overnight against 0 . 5m acetic acid at 4 ° c . with stirring . collagen types were resolved using a c18 column , 300 å pore size ( 250 × 4 . 6 mm ) and elution times of type i and type iii standards were recorded as references . the analysis of this data was complicated by the standards resolving into multiple peaks , with some overlap between type i and type iii peak elution times . to confirm hplc peaks containing type i and type iii collagen , peaks were collected and characterised using sds - page ( 6 % tris - glycine ). from this analysis the area under respective type iii and type i collagen peaks was integrated , and proportions of each collagen type calculated for each tendon . the proportion of type i collagen was expressed as a percentage of the total type i and type iii collagen . the proportion of type i collagen was significantly increased compared to control horses , as shown in table 2 , which compares the percentage of type i collagen in the two treatment groups . table 2 shows the mean percentage of type i collagen and standard error of the mean ( sem ) percentages for type i collagen from the two treatment groups , and the statistical significance ( p ) of igf - i treatment . the means were calculated from individual values for each horse , as determined by hplc analysis of collagen in core lesion samples . the percentages were determined by calculating the area under the curves of hplc chromatographic traces for the core lesion sample from each horse &# 39 ; s tendon , compared to type i and type iii collagen references . an increase in the proportion of type i collagen compared with type iii collagen is indicative of more functionally mature tendon . these two significant results indicate that igf - i treated equine tendinitis heals more rapidly than untreated tendinitis , as shown by the steeper gradients of linear regression for mean grey scale analysis , and more closely resemble mature uninjured tendon in their proportion of type i collagen , which also suggests higher quality repair of tendon tissue treated with igf - i . intralesional injection of igf - i promotes intrinsic repair of acute collagenase - induced tendinitis in horses ; comparisons between doses and frequency of igf - i administration 24 healthy horses with collagenase - induced tendinitis in their nearside forelimbs were used in this study . tendinitis was induced in the same way as described in example 2 . ( 1 ) 250 μg of igf - i formulated in 100 μl of phosphate - buffered saline on each of two occasions seven days apart , ( 2 ) 500 μg of igf - i formulated in 100 μl of phosphate - buffered saline on each of two occasions seven days apart , ( 3 ) a single injection of 500 μg of igf - i formulated in 100 μl of phosphate - buffered saline followed by 100 μl of vehicle seven days later , or ( 4 ) 100 μl of vehicle alone on each of two occasions seven days apart . the ultrasound procedure was carried out as described in example 2 , and the mean grey scale of the treated core lesion within the superficial digital flexor tendon expressed as a percentage of the undamaged deep digital flexor tendon determined at 2 , 4 , 6 and 8 weeks following creation of the lesion . these values are shown in table 3 as means ± sem for the six horses in each group at each time period . regression curves generated from the mean grey scale data analysis in table 3 demonstrate steeper gradients for horses treated with any of the three igf - i protocols than for the vehicle group . no differences were evident between the three igf - i protocols indicating that a dose twice that used in example 2 ( group 2 , example 3 ) gave no additional benefit . importantly , a single injection of igf - i was as effective as when two treatments of igf - i were administered 7 days apart . the percentage of collagen as type 1 collagen in the lesion area was measured as described in example 2 , except that biopsies were obtained 12 weeks after tendinitis was induced . table 4 demonstrates that each of the igf - i treated horse groups had a higher percentage of type 1 collagen than observed in the vehicle - treated group . throughout the treatment and post - treatment phases the lameness of each horse was graded , using an external examination based on a clinically - accepted scale of 0 to 5 , ranging from grade 0 ( completely sound ) to grade 5 ( non - weight bearing lameness ). two independent blinded examiners evaluated horses being trotted and walked on hard level ground , and graded the horse &# 39 ; s lameness based on this scale . the study demonstrates higher negative slopes for the three groups of igf - i - treated horses than for the vehicle group , indicating a more rapid return to normality in the three igf - i - treated groups . as with the healing response and the percentage of type 1 collagen in the lesion area , there were no differences between the three igf - i - treated groups . the person skilled in the art will readily be able to investigate the use of the invention to promote healing of a severed tendon or ligament , for example using the severed tendon model in chickens . suitable chickens for this model include the species gallus domestious . preferably , the birds are anaesthetized with an intramuscular injection , and a foot is washed and then swabbed with a 10 % povidone - iodine solution . using aseptic techniques , an incision 1 - 1 . 5 cm long is made through the plantar surface of the third digit , starting halfway along the first pad distal to the toe webbing , and the subcutaneous fat is cut using iridectomy scissors until the tendon sheath is visible . synovial fluid will escape when the sheath is cut to expose the long digital flexor tendon ( ldft ) as it emerges between the branches of the intermediate flexor tendon ( ift ). to maintain a high moisture content , the surgical field can be irrigated with sterile physiological ( 0 . 9 %) saline . the ldf tendon is raised , and a suture is passed transversely , right to left , through the tendon close to the bifurcation of the ift ; maxon - cv ®, 6 - 0 , polyglyconate , monofilament suture is suitable . a modified kessler stitch is used to appose the two ends of the transected tendon . the igf composition is applied between the two tendon surfaces . preferably the igf is formulated in a fibrin gel . the suture is then tied with even tension , taking care not displace the igf composition . the tendon sheath is closed with an interrupted stitch , followed by closure of the skin with a 4 - 0 silk suture . the toe is wrapped with adhesive bandage , and to prevent rubbing the bandage is placed on the front of the foot and leg up to the knee joint . the chicken &# 39 ; s foot can then be placed in a fibreglass cast specifically designed to maintain the toes straight and bent in flexion approximately 25 ° at the metatarsal / phalangeal joint . cotton wool is used to pack the toes lightly within the cast , which is then tapped to the leg . finally , intramuscular injections of buprenorphine hydrochloride ( 0 . 03 mg / kg ) and amoxicillin / clavulanic acid ( 0 . 2 ml / kg ) are administered , and the birds are allowed to recover from the anaesthetic . at a suitable time following the treatment , preferably five weeks after treatment , the chickens are anaesthetised and then euthanased by the administration of 120 mg / kg of sodium pentobarbitone . various methods are known in the art for assessment and characterisation of the effectiveness of healing of a severed tendon . for example , histology and tensiometry may be used . for histological assessment , preferably the flexor tendon is exposed and freed from the sheath and any excessive adhesive tissue . a piece of tendon approximately 3 centimetres long , evenly spaced each side of the transection , is held flat using biopsy pads inside a histology cassette , then placed into buffered formalin for 48 hours . the tendon is then transferred to 70 % alcohol prior to processing and sectioning . for the assessment of cell density , cellular alignment , neutrophil number and fibroblast alignment , 5 μm sections are stained with haematoxylin / eosin . adjacent 5 μm sections are stained with masson &# 39 ; s trichrome stain , which demonstrates the supporting tissue elements , principally collagen . preferably these qualitative measurements are performed independently and in blinded fashion . for the tensiometry assessment , the left chicken leg is removed at the knee joint on a saline - soaked swab and frozen immediately at − 20 ° c . until all samples for the group have been collected . legs are thawed at 4 ° c . overnight and kept on ice prior to tensiometry . the flexor tendon is removed , measured and the breaking strain tested , for example using a 250n load cell on a mecmesin tensiometer . on the basis of the results shown in example 2 and 3 , the inventors expect that the invention used in this particular model would accelerate the healing of the severed tendon injury and accelerate time to functional use of the tendon as measured by tensiometry , cell density , cellular alignment , neutrophil number , fibroblast alignment and / or lameness scoring . methodologies used to study the effectiveness of igf - i treatments for ligament injuries in dogs the invention may be used to treat a tendon or ligament avulsion . the person skilled in the art will readily be able to investigate the claimed invention to treat a tendon or ligament avulsion . for example , the ligament avulsion model in dogs may be used for the characterisation of the claimed invention for treating ligamental avulsions . the ligament which stabilises the lateral surface of a dog &# 39 ; s toe commonly tears away from the bone at one of its sites of attachment during exercise . this veterinary condition in dogs is commonly referred to as “ sprung toe ”. current practice involves a wide variety of treatments , including limb immobilisation and surgical re - attachment with sutures . in cases undergoing surgical repair accelerated ligament attachment to the bone is preferred , resulting in a higher quality of repair and leading to a greater chance of functional recovery . suitable dogs for this model include the racing greyhound , in which ligament avulsion is common due to excessive exercise . preferably , an igf - i formulation is applied to the site of surgical re - attachment prior to suturing the ligament end into place . for example , the dogs are anaesthetised and the injured foot prepared for surgery . contained in an aseptic field , the injured ligament is exposed through an incision in the lateral side of the toe . once exposed , the damaged end of the ligament is excised and removed . the site of original attachment is examined and surgically prepared , followed by the direct application of an appropriate igf - i formulation . preferably the igf is formulated in a fibrin gel . the surgically prepared ligament end is approximated to its previously prepared original site of attachment and the ligament is initially held in place by a figure of eight suture pattern . on completion of the growth factor treatment and surgical repair , the incision site is sutured and closed , and the dog &# 39 ; s foot splinted and bandaged . preferably , following a period of recuperation of up to 3 months , the extent of healing and the relative effectiveness of igf - i at promoting ligament reattachment is characterised . various methods are known in the art to assess and characterise the effectiveness of healing of a ligament avulsion , including physical examination , histology , tensiometry and walking pattern assessment . for example , examination by palpation may be used . on the basis of the results shown in example 2 and 3 , the inventors expect that the invention used in this particular model would accelerate the healing of the ligamental avulsion injury and accelerate time to functional use of the ligament as measured by palpation , histology , tensiometry and / or lameness scoring . it will be apparent to the person skilled in the art that while the invention has been described in some detail for the purposes of clarity and understanding , various modifications and alterations to the embodiments and methods described herein may be made without departing from the scope of the inventive concept disclosed in this specification . references cited herein are listed on the following pages , and are incorporated herein by this reference . abrahamsson s o , lohmander s . differential effects of insulin - like growth factor - i on matrix and dna synthesis in various regions and types of rabbit tendons . j orthop res 1996 may ; 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