Patent Application: US-76410096-A

Abstract:
recombinant impatiens necrotic spot virus dna constructs comprising an insv dna coding for transcription into insv rna sequences or into rna sequences related thereto , the use of such dna constructs to transform plants having reduced susceptibility to insv infection and probes for the isolation of insv or diagnosis of plant insv related diseases .

Description:
insv isolate nl - 07 , an isolate from impatiens , is maintained on impatiens by grafting . virus is purified from systemically infected nicotiana rustica leaves , after mechanical inoculation essentially as described by tas et al . ( 1977 ) j . gen . virol . 36 : 81 - 91 !. all material used in the isolation procedure should be maintained at a temperature of 4 ° c . twelve days after inoculation 100 grams of infected leaves are harvested and ground for 5 - 10 seconds at a low speed setting in 5 volumes extraction buffer ( 0 . 1m nah 2 po 4 , 0 . 01m na 2 so 3 , ph 7 ) in a waring blender . the suspension is filtered through cheesecloth and the filtrate is centrifuged for 10 minutes at 16 , 000 × g . the resulting pellet is resuspended in three volumes resuspension buffer ( 0 . 01m nah 2 po 4 , 0 . 01m na 2 so 3 , ph 7 ). the pellet is dissolved by stirring carefully at 4 ° c . after centrifuging for 10 minutes at 12 , 500 × g the pellet is discarded and the supernatant centrifuged again for 20 minutes at 50 , 000 × g . the pellet is resuspended in 0 . 2 volume of resuspension buffer ( 0 . 01m nah 2 po 4 , 0 . 01m na 2 so 3 , ph 7 ) and kept on ice for 30 minutes . anti - serum raised in rabbits against material from non - infected nicotiana rustica is added to the solution and carefully stirred for 1 hour . non - viral complexes are pelleted after 10 minutes centrifuging at 16 , 000 × g . the cleared supernatant is loaded on a linear 5 %- 40 % sucrose gradient in resuspension buffer ( 0 . 01m nah 2 po 4 , 0 . 01m na 2 so 3 , ph 7 ), and spun for 45 minutes at 95 , 000 × g . the opalescent band containing insv particles is carefully collected with a syringe and diluted 4 times with resuspension buffer . washed viruses are pelleted by centrifugation for 1 . 5 hours at 21 , 000 × g and resuspended in one volume of resuspension buffer . generally , 100 grams of leaf material yields approximately 0 , 5 mg of insv viruses . insv rna is recovered preferentially from purified virus preparations by sds - phenol extractions followed by ethanol precipitation . from 1 mg insv , 1 - 5 μg of rna is extracted . the isolated rna molecules are analysed for intactness by electrophoresis on an agarose gel . three distinct rna molecules are identified with apparent sizes of about 3000 nucleotides ( s rna ), about 4900 nucleotides ( m rna ) and about 8900 nucleotides ( l rna ) respectively . in order to perform direct rna sequencing , insv rna is extracted from purified nucleocapsids essentially according to verkleij et al . ( 1983 ) supra . twelve days after inoculation 100 grams of infected leaves are harvested and ground for 5 - 10 seconds at a low speed setting in four volumes of tas - e buffer ( 0 . 01m edta , 0 . 01m na 2 so 3 , 0 . 1 % cysteine , 0 . 1m tris ph 8 . 0 ) in a waring blender . the suspension is filtered through cheesecloth and centrifuged for 10 minutes at 1 , 100 × g . nucleocapsids are recovered from the supernatant after 30 minutes of centrifuging at 66 , 000 × g . the pellet is carefully resuspended in one volume of tas - r buffer ( 1 % nonidet np - 40 , 0 . 01m edta , 0 . 01m na 2 so 3 , 0 . 1 % cysteine , 0 . 01m glycine , 0 . 01m tris , ph 7 . 9 ). the pellet is dissolved by stirring carefully for 30 minutes at 4 ° c . the supernatant is cleared by centrifuging for 10 minutes at 16 , 000 × g . crude nucleocapsids are collected from the cleared supernatant by sedimentation through a 30 % sucrose cushion for 1 hour at 105 , 000 × g . the nucleocapsid pellet is resuspended in 400 μl 0 . 01m na - citrate ph 6 . 5 , layered on a 20 - 40 % sucrose ( in 0 . 01m na - citrate ph 6 . 5 ) and spun for 2 hours at 280 , 000 × g . the three different opalescent bands , respectively l , m and s nucleocapsid , are collected separately . insv rna is recovered preferentially from purified nucleocapsid preparations by sds - phenol extractions followed by ethanol precipitation . generally , 100 μg of rna are obtained from 100 grams of infected leaves . the 3 &# 39 ;- ends of the separate insv rnas are labeled using rna ligase and 5 &# 39 ;- 32 p ! pcp . the end - labeled rna molecules are separated on a low gelling temperature agarose gel wieslander , ( 1979 ) anal biochem 98 : 305 - 309 !. the enzymatic approach described by clerx - van haaster and bishop ( 1980 ) virology 105 : 564 - 574 ! and clerx - van haaster et al . ( 1982 ) j gen virol 61 : 289 - 292 ! is used to determine the 30 terminal nucleotides of the 3 &# 39 ;- and 5 &# 39 ;- ends of both s and m rna . synthetic oligonucleotides complementary to the 3 &# 39 ;- termini are synthesized using a commercially available system ( applied biosystems ) and used for dideoxy - sequencing with reverse transcriptase . oligonucleotides complementary to the 3 &# 39 ;- end of the s rna are used for priming first strand cdna synthesis . with these primers , double stranded dna to insv rna is synthesized according to gubler and hoffman ( 1983 ) gene 25 : 263 - 269 !. two different approaches are used to generate cdna clones to the insv viral rnas . a first series of clones is obtained by random priming of the insv rna using fragmented single stranded calf thymus dna , followed by first and second strand cdna synthesis . cdna is made blunt - ended using t4 - dna polymerase and ligated with t4 ligase into the smai site of puc19 . a second series of insv cdna clones is obtained by priming first strand dna synthesis with the oligonucleotides complementary to the 20 terminal nucleotides at the 3 &# 39 ;- ends of the insv rnas . blunt ended cdna fragments are cloned into the sma i site of puc19 . cdna clones from both series containing viral inserts are selected via colony hybridization , essentially according to the method of grunstein and hogness ( 1975 ) proc . natl . acad . sci . usa 72 : 3961 - 3965 ! using 32 ! p - labeled , randomly primed first strand cdna as a probe . sets of overlapping cdna clones are selected by southern analysis followed by plasmid walking , in order to construct a restriction map , based on cdna derived sequences of the s rna ( fig2 ). in order to determine the sequence of the s rna 5 selected cdna clones are subcloned into pbluescript , resulting in the plasmids pinsv - s2 , pinsv - s15 , pinsv - s61 , pinsv - s60 and pinsv - s39 , ( fig2 ). the clones are sequenced in both directions using the protocol of zhang et al . ( 1988 ) nucl . acids . res . 16 : 1220 !. the nucleotide sequence of the 3 &# 39 ;- end of the s rna is determined by primer extension of the synthetic oligonucleotide insv - s60 ( 5 &# 39 ; d ( agagcaattgtgtca ) which is complementary to the 15 nucleotides of the 3 &# 39 ;- terminus . sequence data from the insv s rna ( 3017 nt ) is summarized in the sequence listing ( seq id no . 1 to seq id no . 12 ). computer simulated translation of the 6 different reading frames on the viral strand and viral complementary strand reveals the presence of two putative open reading frames ( fig3 ). on the viral strand an open reading frame is found starting at position 87 and terminating at a uaa stopcodon at position 1436 encoding a protein of 449 amino acids with a predicted molecular mass of about 51 . 2 kd . this protein is a non - structural protein , tentatively designated nss ( fig3 / seq id no . 26 ). the other open reading frame is located on the viral complementary strand from position 2080 to 2868 ( seq id no . 11 ), encoding a 262 amino acid long polypeptide with a predicted molecular mass of about 28 . 7 kd . this open reading frame encodes the viral nucleocapsid protein n ( fig3 / seq id no 25 ). thus fig3 shows the coding capacities of the viral and the viral complementary strand of insv s rna , indicating the nss and n protein genes are expressed from subgenomic mrnas ( seq id no . 3 , seq id no . 11 respectively ). thus , the situation occurs that a plant virus rna has an ambisense gene arrangement . other important features of this s rna sequence is the existence of complementary terminal repeats capable of forming so - called &# 34 ; pan - handle &# 34 ; structures . these structures play an important role in replication and transcription of viral rna . another putative regulatory element is the secondary structure in the intergenic region of the s rna , which most likely contains the transcription termination signals for both subgenomic mrnas , encoding respectively the n and nss - protein . the nucleotide sequence of the insv m and l rna is elucidated employing similar strategies and methods as used to determine the nucleotide sequence of the s rna . the recombinant plasmid pzo347 is a derivative of pbluescript carrying a 496 bp bamhi - smai fragment containing a 426 bp 35s promoter fragment ( hincii fragment ) of camv strain cabb - s , linked to a 67 bp fragment of the non - translated leader region , the so - called ω - region , of the tobacco mosaic virus . this results in a chimeric promoter with a complete transcriptional fusion between the promoter of camv to the untranslated leader of tmv . by using in vitro mutagenesis the original position of the tmv atg startcodon is mutated to a smai site . the plasmid pzo008 carries the nopaline synthase ( nos ) terminator as a 260 bp psti - hindiii fragment . this psti - hindiii fragment is excised from pzo008 and ligated using t4 ligase into psti - hindiii linearized pzo347 . the resulting recombinant plasmid pzu - b is another plant expression vector . the sequence of this 35s - ω promoter as used in the plant expression vector pzu - b is shown as seq id no . 23 . the resulting recombinant plasmid pzu - b contains the 35s hincii - tmv ω fusion ( 35s - ω ), unique smai and psti sites and the nos terminator ( fig4 ). this expression vector is preferentially used in constructing translational fusions of the gene for expression downstream of the chimaeric promoter 35s - ω . the insv n protein coding sequence is obtained by fusion of the cdna clones pinsv - s60 and pinsv - s39 ( fig5 ). the cdna clone pinsv - s60 is subjected to spei digestion and the fragment containing the 3 &# 39 ;- end of the insv n protein gene is separated electrophoretically and purified from the gel using a deae membrane ( na - 45 , schleicher and schull ) and cloned in the largest spei fragment of pinsv - s39 linearized resulting in the recombinant plasmid pinsv - n . primers are designed homologous to the translational start and stop codon . primer insv - 066 d ( gcagatatcatgaacaaagc ) creates an ecorv site just proximal to the start codon . primer insv - 070 d ( gcaacctgcagctcaaatctctt ) creates a psti site just distal to the stop codon . these primers are used in standard pcr experiments in which pinsv - n is used as the template . the resulting pcr fragment is isolated from the gel using a deae membrane ( na - 45 , schleicher and schull ) and cloned in the smai linearized pbluescript to generate plasmid pinsv - n2 . the added restriction sites , ecorv and psti , facilitate the construction of further plasmids . ( alternatively , one may choose to add the sites in different ways such as but not limited to site - directed mutagenesis or by ligation of other synthetic oligonucleotide linkers . such methods are all known to a person skilled in the art .) subcloning of the insv non - structural protein genes ( nss gene ) of insv s rna the sequence of the gene corresponding to the non - structural protein nss is isolated using rna based pcr on isolated insv s rna . two primers are designed which are homologous to regions spanning either the translational start codon or stop codon . the start codon primer contains an ecorv site proximal to the atg codon , the stop codon primer has a psti site just distal thereto . purified insv s rna is subjected to the gene amp rna pcr . the resulting pcr fragment is isolated from the gel and cloned into smai linearized pbluescript yielding the recombinant plasmid pinsv - nss ( fig6 ). subcloning of the insv non - structural protein gene ( nsm gene ) of the insv m rna the sequence of the gene corresponding to the non - structural protein nsm is isolated using rna based pcr on isolated insv m rna . two primers are designed which are homologous to regions spanning either the translational start codon or stop codon . the start codon primer contains an ecorv site proximal to the atg codon , the stop codon primer has a psti site just distal thereto . purified insv s rna is subjected to the gene amp rna pcr . the resulting pcr fragment is isolated from the gel and cloned into smai linearized pbluescript yielding the recombinant plasmid pinsv - nsm ( fig7 ). subcloning of the insv g1 / g2 glycoprotein gene ( g1 / g2 gene ) of the insv m rna the sequence of the gene corresponding to the g1 / g2 glycoprotein precursor is isolated using rna based pcr on isolated insv m rna . two primers are designed homologous to regions spanning either the translational start codon or stop codon . the start codon primer contains an ecorv site proximal to the atg codon , the stop codon primer has a psti site just distal thereto . purified insv m rna is subjected to the gene amp rna pcr . the resulting pcr fragment is isolated from the gel and cloned into smai linearized pbluescript yielding the recombinant plasmid pinsv - g1 / g2 ( fig8 ). in order to make a fusion in which the atg start codon from the n protein gene is fused directly to the 3 &# 39 ;- end of the tmv untranslated leader of the 35s - ω promoter the start codon of the n gene has to be mutated using the pcr approach as hereinbefore described . the n protein gene is excised from the plasmid pinsv - n2 via an ecorv - psti digestion . the fragment is isolated and inserted into the smai - psti linearised pzu - b , resulting in recombinant plasmid pinsv - nb . the chimeric cassette containing the 35s - ω promoter , the n gene and the nos terminator is excised from the plasmid pinsv - nb via a bamhi / xbai digestion . the isolated chimaeric gene cassette is then inserted into the bamhi / xbai linearized pbin19 to create the binary transformation vector pinsv - nbb . the resulting plasmid pinsv - nbb ( fig9 ) is used in plant transformation experiments using methods well known to a person skilled in the art . in order to create a fusion in which the atg start codon from the nss protein is fused directly to the 3 &# 39 ;- end of the tmv leader of the 35s - ω promoter the start codon of the nss gene is mutated , using the pcr approach . the plasmid pinsv - ns is digested with ecorv and psti and the nss containing fragment is isolated from the gel and inserted into smai / psti linearized pzu - b resulting in the recombinant plasmid pinsv - nssb . the chimaeric cassette containing the 35s - ω promoter , the mutated nss protein gene and the nos terminator is excised from the plasmid pinsv - nssb via a bamhi / xbai digestion . the isolated chimeric gene cassette is then inserted into the bamhi / xbai linearized pbin19 to create the binary transformation vector pinsv - nssbb . the resulting plasmid pinsv - nssbb ( fig1 ) is used in plant transformation experiments using methods well known to a person skilled in the art . in order to create a fusion in which the atg start codon from the g1 / g2 glycoproteinprecursor is fused directly to the 3 &# 39 ;- end of the tmv leader of the 35s - ω promoter the start codon of the g1 / g2 gene is mutated , using the pcr approach . the plasmid pinsv - g1 / g2 is digested with ecorv and psti and the g1 / g2 containing fragment is isolated from the gel and inserted into smai / psti linearized pzu - b resulting in the recombinant plasmid pinsv - g1 / g2b . the chimeric cassette containing the 35s - ω promoter , the mutated g1 / g2 glycoprotein gene and the nos terminator is excised from the plasmid pinsv - g1 / g2b via a bamhi / xbai digestion . the isolated chimeric gene cassette is then inserted into the bamhi / xbai linearized pbin19 to create the binary transformation vector pinsv - g1 / g2bb . the resulting plasmid pinsv - g1 / g2bb ( fig1 ) is used in plant transformation experiments using methods well known to a person skilled in the art . in order to create a fusion in which the atg start codon from the nsm protein is fused directly to the 3 &# 39 ;- end of the tmv leader of the 35s - ω promoter the startcodon of the nsm gene is mutated , using the pcr approach . the plasmid pinsv - nsm is digested with ecorv and psti and the nsm - containing fragment is isolated from the gel and inserted into smai / psti linearized pzu - b resulting in the recombinant plasmid pinsv - nsmb . the chimeric cassette containing the 35s - ω promoter , the mutated nsm protein gene and the nos terminator is excised from the plasmid pinsv - nsmb via a bamhi / xbai digestion . the isolated chimeric gene cassette is then inserted into the bamhi / xbai linearized pbin19 to create the binary transformation vector pinsv - nsmbb . the resulting plasmid pinsv - nsmbb ( fig1 ) is used in plant transformation experiments using methods well known to a person skilled in the art . a dna analysis programme is used to locate the &# 34 ; pan - handle &# 34 ; element of the loop in the viral insv s rna . the strongest &# 34 ; pan - handle &# 34 ; structure that is detected includes about the first 24 - 25 nucleotides at the 5 &# 39 ;- end ( 1 to 24 or 25 ) of the viral s rna and about the last 36 nucleotides at the 3 &# 39 ;- end of the viral s rna ( seq id nos 5 and 6 respectively ). the length of the pan - handle element of the loop is about 36 nucleotides long . these regions are synthesized on a commercial dna synthesizer and appropriate linker sequences are added . construction of the &# 34 ; pan - handle &# 34 ; vectors of s and m rna results in respectively : pinsv - terms and pinsv - termm . using appropriate restriction enzyme combination these fragments are inserted between the 35s - ω promoter and the nos terminator of pzu - b yielding the chimeric cassettes : pinsv - termsa , pinsv - termma , pinsv - termsb and pinsv - termmb . these cassettes are then transferred into the binary transformation vector pbin19 using appropriate enzyme combinations yielding the following plasmids : pinsv - termsab , pinsv - termmab , pinsv - termsbb and pinsv - termmbb . alternatively , it is possible to design &# 34 ; pan - handle &# 34 ; constructs including the 3 &# 39 ;- and 5 &# 39 ;- end termini that are larger than indicated above , or separated by any other dna sequence in order to enhance the stability of the transcripts produced from these recombinant genes in plants . all &# 34 ; pan - handle &# 34 ; constructs resemble shortened tospovirus rna molecules , specifically insv rna molecules and therefore can be regarded as defective interfering rnas . a dna analysis programme is used to locate a secondary structure in the viral insv s rna . the strongest secondary structure detectable starts at nucleotide 1440 and ends at nucleotide 2041 of seq id no . 1 , ( seq id no 8 ). the dna fragment carrying the secondary structure region is isolated from pinsv - s61 using a pcr approach similar to that described earlier . the two primers used contain the sequences 1440 - 1460 and 2021 - 2041 of seq id no . 1 . the pcr fragment is excised from an agarose gel and subsequently treated with t4 polymerase to create blunt ends and is subsequently cloned into the smai site of the expression vector pzu - b , resulting in the recombinant plasmid pinsv - hpsb . the plasmid pinsv - hpsb is digested with hindiii and the fragment containing the chimeric gene is excised from an agarose gel and ligated into xbai linearized pbin19 , resulting in the transformation vector pinsv - hpsbb . ( it is clear to a person skilled in the art that other fragments can be isolated from the cdna clones of the insv s rna containing the hairpin region as described above without interference to function . also , a fragment containing the hairpin region may be synthesized using a dna - synthesizer .) methods to transfer binary vectors to plant material are well established and known to a person skilled in the art . variations in procedures exist due to for instance differences in used agrobacterium strains , different sources of explant material , differences in regeneration systems depending on as well the cultivar as the plant species used . the binary plant transformation vectors as described above are used in plant transformation experiments according to the following procedures . the constructed binary vector is transferred by tri - parental mating to an acceptor agrobacterium tumefaciens strain , followed by southern analysis of the ex - conjugants for verification of proper transfer of the construct to the acceptor strain , inoculation and cocultivation of axenic explant material with the agrobacterium tumefaciens strain of choice , selective killing of the agrobacterium tumefaciens strain used with appropriate antibiotics , selection of transformed cells by growing on selective media containing kanamycine , transfer of tissue to shoot - inducing media , transfer of selected shoots to root inducing media , transfer of plantlets to soil , assaying for intactness of the construct by southern analyses of isolated total dna from the transgenic plant , assaying for proper function of the inserted chimeric gene by northern analysis and / or enzyme assays and western blot analysis of proteins . rna is extracted from leaves of regenerated plants using the following protocol . grind 200 mg leaf material to a fine powder in liquid nitrogen . add 800 μl rna extraction buffer ( 100 mm tris - hcl ( ph 8 , 0 ), 500 mm nacl , 2 mm edta , 200 mm β - mercapto - ethanol , 0 , 4 % sds ) and extract the homogenate with phenol , collect the nucleic acids by alcohol precipitation . resuspend the nucleic acids in 0 , 5 ml 10 mm tris - hcl ( ph 8 , 0 ), 1 mm edta , add licl to a final concentration of 2m , leave on ice for maximal 4 hours and collect the rna by centrifugation . resuspend in 400 μl 10 mm tris - hcl ( ph 8 , 0 ), 1 mm edta and precipitate with alcohol , finally resuspend in 50 μl 10 mm tris - hcl ( ph 8 , 0 ), 1 mm edta . rnas are separated on glyoxal / agarose gels and blotted to genescreen as described by van grinsven et al . ( 1986 ) theor appl gen 73 : 94 - 101 !. insv s rna sequences are detected using dna or rna probes labeled with 32 p !, 35 s ! or by using non - radioactive labeling techniques . based on northern analysis , it is determined to what extent the regenerated plants express chimaeric insv s rna sequences . plants transformed with chimaeric constructs containing an insv n protein - encoding sequence are also subjected to western blot analysis . proteins are extracted from leaves of transformed plants by grinding in sample buffer according to the method of laemmli ( 1970 ) nature 244 : 29 - 30 !. a 50 μg portion of protein is subjected to electrophoresis in a 12 , 5 % sds - polyacrylamide gel essentially as described by laemmli ( 1970 ) supra . separated proteins are transferred to nitrocellulose electrophoretically as described by towbin et al . ( 1979 ) proc . natl . acad . sci . usa 76 : 4350 - 4354 !. transferred proteins are reacted with antiserum raised against purified insv structural or non - structural proteins ( towbin et al . ( 1979 ) supra . based on the results of the western analysis , it is determined that transformed plants do contain insv n proteins encoded by the inserted chimaeric sequences . transformed plants are grown in the greenhouse under standard quarantine conditions in order to prevent any infections by pathogens . the transformants are self - pollinated and the seeds harvested . progeny plants are analyzed for segregation of the inserted gene and subsequently infected with insv by mechanical inoculation . tissue from plants systemically infected with insv is ground in 5 volumes of ice - cold inoculation buffer ( 10 mm phosphate buffer supplemented with 1 % na 2 so 3 ) and rubbed in the presence of carborundum powder on the first two fully extended leafs of approximately 5 weeks old seedlings . inoculated plants are monitored for symptom development during 3 weeks after inoculation . plants containing insv related dna sequences show reduced susceptibility to insv infection as exemplified by a delay in symptom development , whereas untransformed control plants show severe systemic insv symptoms within 7 days after inoculation . rna is extracted from leaves of suspected plants using the following protocol : grind 1 gram of leaf material , preferentially showing disease symptoms , in 3 ml 100 mm tris - hcl , 50 mm edta , 1 . 5m nacl and 2 % ctab ( ph 8 . 0 ). after grinding , 1 ml of the homogenate is subjected to chloroform extraction and incubated at 65 ° c . for 10 minutes . the inorganic phase is then collected and extracted with phenol / chloroform ( 1 : 1 ), followed by a last extraction with chloroform . the ribonucleic acids are isolated from the inorganic phase , containing the total nucleic acids , by adding licl to a final concentration of 2m . the preparation is incubated at 4 ° c . for 1 hour , after which the ribonucleic acids are collected by centrifugation . the ribonucleic acid pellet is resuspended in 25 μl 10 mm tris - hcl , 1 mm edta ( ph 8 . 0 ). the ribonucleic acids are recovered by standard alcohol precipitation . the ribonucleic acid pellet is resuspended in 25 μl 10 mm tris - hcl , 1 mm edta ( ph 8 . 0 ). 1 μl of the purified ribonucleic acids is spotted on a nylon blotting membrane ( e . g . hybond - n , amersham uk ). the presence of insv in the plant is detected by standard hybridization , using any part or parts of the sequence isolated from virions or preferentially by designing synthetic oligomers on the basis of disclosed sequence information as a probe . ( alternatively , in vitro transcripts of regions of the insv genome are used to detect insv related rna sequences in diseased plants .) a diseased plant is diagnosed by the occurrence of hybridization at the dot containing rna material from the diseased plant . __________________________________________________________________________sequence listing ( 1 ) general information :( iii ) number of sequences : 27 ( 2 ) information for seq id no : 1 :( i ) sequence characteristics :( a ) length : 3001 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 1 : agagcaatgaacaaccaagctacaacaaatcttacaatattgtcaattacattactactt60ccattttaacatgtctagtgcaatgtatgaaacaattatcaaatcgaagtcctcaatctg120gggaacaacatcttcgggtaaagcagtagtagatagttattggattcatgatcaatcttc180cggaaagaagttggtcgaagctcaactctattctgactccaggagcaagaccagtttctg240ttacactggtaaagttggctttctcccaacagaagaaaaagaaattatagtgagatgttt300tgtgcctatttttgatgacattgatctgaatttctccttttcagggaatgttgtcgaaat360tctggtcagatctaacacaacaaacacaaacggtgttaaacatcaaggtcatctcaaagt420gttatcctctcagttgctcagaatgcttgaagagcaaatagcagtgcctgaaattacttc480aagattcggtctgaaagaatctgacatcttccctccaaataatttcattgaagctgcaaa540taaaggatcattgtcttgtgtcaaagaagtcctttttgatgtcaagtattcaaacaacca600atccatgggcaaagtcagtgttctttctcctaccagaagtgttcatgaatggctgtacac660acttaagcctgtttttaaccaatcccagaccaacaacaggacagtaaacactttggctgt720aaaatcactggcaatgtctgcaacttctgatttaatgtcagatactcattcgtttgtcag780gctcaataataacaagccttttaaaatcagcctttggatgcgcatccctaaaataatgaa840atcaaacacatacagccggttcttcaccctgtctgatgaatcttctcctaaagagtatta900tataagcattcaatgtcttccgaatcacaacaatgttgaaacagtcattgaatataactt960tgatcagtcaaacctcttcttgaatcaactccttctagcagtgattcataaaattgagat1020gaatttttctgatctaaaagaaccttacaatgttatccatgatatgtcgtatcctcaaag1080aattgttcattcacttcttgaaatccacacagaacttgctcaaactgtctgtgacagtgt1140tcagcaagacatgattgtcttcactataaatgagccagatctaaagccaaaaaagtttga1200gctagggaaaaagactttaaattattcagaagatggttatgggagaaaatatttcctttc1260tcagaccttgaaaagtcttccgagaaactcacaaacaatgtcttatttggatagcatcca1320gatgcccgattggaaatttgactatgctgcaggtgaaataaaaatttctcctagatcaga1380ggatgttttgaaagctatttctaaattagatttaaattaaccttggttaaacttgtccct1440aagtaaagtttgtttacatgcatttagatcagattaaacaaatctaataacagataaacc1500aaaaacaatcatatgaaataaataaataaacataaaatatataaaaaatacaaaaaaaat1560cataaaataaataaaaaccaaaaaaggatggccttcgggcacaatttggttgctttaata1620atgctttaaaatgaatgtattagtaaattataaactttaaatccaatctactcacaaatt1680ggccaaaaatttgtatttgtttttgtttttgttttttgttttttgtttttgttttgtttt1740atttgttttttattttgttttttgttttttgttttttattttatttatatatatatatat1800atatattttgtagtggtttttattgtttttattattttttgtagcttttttacttgttta1860tttcacacgcaaacacactttcaagtttatatattaaaacacacattaaacttatttcaa1920ataatttataaaagcacacttaatacactcaaacaataattaattattttattttttatt1980ttattttttatttttattatttttatttttatttatttaaatgcatttaacacaacacaa2040agcaaaccaagctcaaatctcttttaaatagaatcatttttcccaaaatcaatagtagca2100ttaaacatgctgtaaatggatgtaagcccttctttgtagtggtccattgcagcaagtcct2160ttagctttcggactacaagcctttagtatatctgcatattgtttagccttgccaatttca2220acagagttcatgctatatcctttgctttttagaactgtgcacactttcccaactgcctct2280ttagtgctaaacttagacatgtcaattccaagctcaacatgtttagcatcttgataaata2340gccggaactagtgcagctatttcaaaattcagtacagatgctatcagaggaagacttcct2400cctaagagaacacccaagacacaggatttcaaatctgtggttgcaagaccatatgaggca2460atcagagggtgacttggaaggctatttatagcttcagtcagagcagatccattgtccttt2520atcattccaacaagatgaactctcaccattgcatcaagtcttcggaaagtcatatcattg2580accccaactctttctgaattgtttctagttttcttaattgtgactgatccaaaagtgaag2640tcagcactcttaatgactctcattatagattgcctattcttgaggaaggataggcaggat2700gcagtagtcatgttctgaatcttttcacggttgttggtaaagaagtcagtgaaattgaaa2760gacccttcattttgagtttcctcaaattctaaggaatcagattgagtcaaaagcttgact2820atgttctccttggtaatctttgctttgttcatcttgatctgctgactttactaactttaa2880agcttaaagtgttcaaattactaaatagtacttgcggttaaagtagtatttggtaaaatt2940tgtaatttttcagtttctagctttggattatatgatgttatattcgtgacacaattgctc3000t3001 ( 2 ) information for seq id no : 2 :( i ) sequence characteristics :( a ) length : 2993 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 2 : gaacaaccaagctacaacaaatcttacaatattgtcaattacattactacttccatttta60acatgtctagtgcaatgtatgaaacaattatcaaatcgaagtcctcaatctggggaacaa120catcttcgggtaaagcagtagtagatagttattggattcatgatcaatcttccggaaaga180agttggtcgaagctcaactctattctgactccaggagcaagaccagtttctgttacactg240gtaaagttggctttctcccaacagaagaaaaagaaattatagtgagatgttttgtgccta300tttttgatgacattgatctgaatttctccttttcagggaatgttgtcgaaattctggtca360gatctaacacaacaaacacaaacggtgttaaacatcaaggtcatctcaaagtgttatcct420ctcagttgctcagaatgcttgaagagcaaatagcagtgcctgaaattacttcaagattcg480gtctgaaagaatctgacatcttccctccaaataatttcattgaagctgcaaataaaggat540cattgtcttgtgtcaaagaagtcctttttgatgtcaagtattcaaacaaccaatccatgg600gcaaagtcagtgttctttctcctaccagaagtgttcatgaatggctgtacacacttaagc660ctgtttttaaccaatcccagaccaacaacaggacagtaaacactttggctgtaaaatcac720tggcaatgtctgcaacttctgatttaatgtcagatactcattcgtttgtcaggctcaata780ataacaagccttttaaaatcagcctttggatgcgcatccctaaaataatgaaatcaaaca840catacagccggttcttcaccctgtctgatgaatcttctcctaaagagtattatataagca900ttcaatgtcttccgaatcacaacaatgttgaaacagtcattgaatataactttgatcagt960caaacctcttcttgaatcaactccttctagcagtgattcataaaattgagatgaattttt1020ctgatctaaaagaaccttacaatgttatccatgatatgtcgtatcctcaaagaattgttc1080attcacttcttgaaatccacacagaacttgctcaaactgtctgtgacagtgttcagcaag1140acatgattgtcttcactataaatgagccagatctaaagccaaaaaagtttgagctaggga1200aaaagactttaaattattcagaagatggttatgggagaaaatatttcctttctcagacct1260tgaaaagtcttccgagaaactcacaaacaatgtcttatttggatagcatccagatgcccg1320attggaaatttgactatgctgcaggtgaaataaaaatttctcctagatcagaggatgttt1380tgaaagctatttctaaattagatttaaattaaccttggttaaacttgtccctaagtaaag1440tttgtttacatgcatttagatcagattaaacaaatctaataacagataaaccaaaaacaa1500tcatatgaaataaataaataaacataaaatatataaaaaatacaaaaaaaatcataaaat1560aaataaaaaccaaaaaaggatggccttcgggcacaatttggttgctttaataatgcttta1620aaatgaatgtattagtaaattataaactttaaatccaatctactcacaaattggccaaaa1680atttgtatttgtttttgtttttgttttttgttttttgtttttgttttgttttatttgttt1740tttattttgttttttgttttttgttttttattttatttatatatatatatatatatattt1800tgtagtggtttttattgtttttattattttttgtagcttttttacttgtttatttcacac1860gcaaacacactttcaagtttatatattaaaacacacattaaacttatttcaaataattta1920taaaagcacacttaatacactcaaacaataattaattattttattttttattttattttt1980tatttttattatttttatttttatttatttaaatgcatttaacacaacacaaagcaaacc2040aagctcaaatctcttttaaatagaatcatttttcccaaaatcaatagtagcattaaacat2100gctgtaaatggatgtaagcccttctttgtagtggtccattgcagcaagtcctttagcttt2160cggactacaagcctttagtatatctgcatattgtttagccttgccaatttcaacagagtt2220catgctatatcctttgctttttagaactgtgcacactttcccaactgcctctttagtgct2280aaacttagacatgtcaattccaagctcaacatgtttagcatcttgataaatagccggaac2340tagtgcagctatttcaaaattcagtacagatgctatcagaggaagacttcctcctaagag2400aacacccaagacacaggatttcaaatctgtggttgcaagaccatatgaggcaatcagagg2460gtgacttggaaggctatttatagcttcagtcagagcagatccattgtcctttatcattcc2520aacaagatgaactctcaccattgcatcaagtcttcggaaagtcatatcattgaccccaac2580tctttctgaattgtttctagttttcttaattgtgactgatccaaaagtgaagtcagcact2640cttaatgactctcattatagattgcctattcttgaggaaggataggcaggatgcagtagt2700catgttctgaatcttttcacggttgttggtaaagaagtcagtgaaattgaaagacccttc2760attttgagtttcctcaaattctaaggaatcagattgagtcaaaagcttgactatgttctc2820cttggtaatctttgctttgttcatcttgatctgctgactttactaactttaaagcttaaa2880gtgttcaaattactaaatagtacttgcggttaaagtagtatttggtaaaatttgtaattt2940ttcagtttctagctttggattatatgatgttatattcgtgacacaattgctct2993 ( 2 ) information for seq id no : 3 :( i ) sequence characteristics :( a ) length : 1350 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 3 : atgtctagtgcaatgtatgaaacaattatcaaatcgaagtcctcaatctggggaacaaca60tcttcgggtaaagcagtagtagatagttattggattcatgatcaatcttccggaaagaag120ttggtcgaagctcaactctattctgactccaggagcaagaccagtttctgttacactggt180aaagttggctttctcccaacagaagaaaaagaaattatagtgagatgttttgtgcctatt240tttgatgacattgatctgaatttctccttttcagggaatgttgtcgaaattctggtcaga300tctaacacaacaaacacaaacggtgttaaacatcaaggtcatctcaaagtgttatcctct360cagttgctcagaatgcttgaagagcaaatagcagtgcctgaaattacttcaagattcggt420ctgaaagaatctgacatcttccctccaaataatttcattgaagctgcaaataaaggatca480ttgtcttgtgtcaaagaagtcctttttgatgtcaagtattcaaacaaccaatccatgggc540aaagtcagtgttctttctcctaccagaagtgttcatgaatggctgtacacacttaagcct600gtttttaaccaatcccagaccaacaacaggacagtaaacactttggctgtaaaatcactg660gcaatgtctgcaacttctgatttaatgtcagatactcattcgtttgtcaggctcaataat720aacaagccttttaaaatcagcctttggatgcgcatccctaaaataatgaaatcaaacaca780tacagccggttcttcaccctgtctgatgaatcttctcctaaagagtattatataagcatt840caatgtcttccgaatcacaacaatgttgaaacagtcattgaatataactttgatcagtca900aacctcttcttgaatcaactccttctagcagtgattcataaaattgagatgaatttttct960gatctaaaagaaccttacaatgttatccatgatatgtcgtatcctcaaagaattgttcat1020tcacttcttgaaatccacacagaacttgctcaaactgtctgtgacagtgttcagcaagac1080atgattgtcttcactataaatgagccagatctaaagccaaaaaagtttgagctagggaaa1140aagactttaaattattcagaagatggttatgggagaaaatatttcctttctcagaccttg1200aaaagtcttccgagaaactcacaaacaatgtcttatttggatagcatccagatgcccgat1260tggaaatttgactatgctgcaggtgaaataaaaatttctcctagatcagaggatgttttg1320aaagctatttctaaattagatttaaattaa1350 ( 2 ) information for seq id no : 4 :( i ) sequence characteristics :( a ) length : 789 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 4 : ttaaatagaatcatttttcccaaaatcaatagtagcattaaacatgctgtaaatggatgt60aagcccttctttgtagtggtccattgcagcaagtcctttagctttcggactacaagcctt120tagtatatctgcatattgtttagccttgccaatttcaacagagttcatgctatatccttt180gctttttagaactgtgcacactttcccaactgcctctttagtgctaaacttagacatgtc240aattccaagctcaacatgtttagcatcttgataaatagccggaactagtgcagctatttc300aaaattcagtacagatgctatcagaggaagacttcctcctaagagaacacccaagacaca360ggatttcaaatctgtggttgcaagaccatatgaggcaatcagagggtgacttggaaggct420atttatagcttcagtcagagcagatccattgtcctttatcattccaacaagatgaactct480caccattgcatcaagtcttcggaaagtcatatcattgaccccaactctttctgaattgtt540tctagttttcttaattgtgactgatccaaaagtgaagtcagcactcttaatgactctcat600tatagattgcctattcttgaggaaggataggcaggatgcagtagtcatgttctgaatctt660ttcacggttgttggtaaagaagtcagtgaaattgaaagacccttcattttgagtttcctc720aaattctaaggaatcagattgagtcaaaagcttgactatgttctccttggtaatctttgc780tttgttcat789 ( 2 ) information for seq id no : 5 :( i ) sequence characteristics :( a ) length : 21 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 5 : agagcaatgaacaacccaagc21 ( 2 ) information for seq id no : 6 :( i ) sequence characteristics :( a ) length : 36 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 6 : gattatatgatgttatattcgtgacacaattgctct36 ( 2 ) information for seq id no : 7 :( i ) sequence characteristics :( a ) length : 643 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 7 : ccttggttaaacttgtccctaagtaaagtttgtttacatgcatttagatcagattaaaca60aatctaataacagataaaccaaaaacaatcatatgaaataaataaataaacataaaatat120ataaaaaatacaaaaaaaatcataaaataaataaaaaccaaaaaaggatggccttcgggc180acaatttggttgctttaataatgctttaaaatgaatgtattagtaaattataaactttaa240atccaatctactcacaaattggccaaaaatttgtatttgtttttgtttttgttttttgtt300ttttgtttttgttttgttttatttgttttttattttgttttttgttttttgttttttatt360ttatttatatatatatatatatatattttgtagtggtttttattgtttttattatttttt420gtagcttttttacttgtttatttcacacgcaaacacactttcaagtttatatattaaaac480acacattaaacttatttcaaataatttataaaagcacacttaatacactcaaacaataat540taattattttattttttattttattttttatttttattatttttatttttatttatttaa600atgcatttaacacaacacaaagcaaaccaagctcaaatctctt643 ( 2 ) information for seq id no : 8 :( i ) sequence characteristics :( a ) length : 602 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 8 : tggttaaacttgtccctaagtaaagtttgtttacatgcatttagatcagattaaacaaat60ctaataacagataaaccaaaaacaatcatatgaaataaataaataaacataaaatatata120aaaaatacaaaaaaaatcataaaataaataaaaaccaaaaaaggatggccttcgggcaca180atttggttgctttaataatgctttaaaatgaatgtattagtaaattataaactttaaatc240caatctactcacaaattggccaaaaatttgtatttgtttttgtttttgttttttgttttt300tgtttttgttttgttttatttgttttttattttgttttttgttttttgttttttatttta360tttatatatatatatatatatattttgtagtggtttttattgtttttattattttttgta420gcttttttacttgtttatttcacacgcaaacacactttcaagtttatatattaaaacaca480cattaaacttatttcaaataatttataaaagcacacttaatacactcaaacaataattaa540ttattttattttttattttattttttatttttattatttttatttttatttatttaaatg600ca602 ( 2 ) information for seq id no : 9 :( i ) sequence characteristics :( a ) length : 3000 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 9 : agagcaattgtgtcacgaatataacatcatataatccaaagctagaaactgaaaaattac60aaattttaccaaatactactttaaccgcaagtactatttagtaatttgaacactttaagc120tttaaagttagtaaagtcagcagatcaagatgaacaaagcaaagattaccaaggagaaca180tagtcaagcttttgactcaatctgattccttagaatttgaggaaactcaaaatgaagggt240ctttcaatttcactgacttctttaccaacaaccgtgaaaagattcagaacatgactactg300catcctgcctatccttcctcaagaataggcaatctataatgagagtcattaagagtgctg360acttcacttttggatcagtcacaattaagaaaactagaaacaattcagaaagagttgggg420tcaatgatatgactttccgaagacttgatgcaatggtgagagttcatcttgttggaatga480taaaggacaatggatctgctctgactgaagctataaatagccttccaagtcaccctctga540ttgcctcatatggtcttgcaaccacagatttgaaatcctgtgtcttgggtgttctcttag600gaggaagtcttcctctgatagcatctgtactgaattttgaaatagctgcactagttccgg660ctatttatcaagatgctaaacatgttgagcttggaattgacatgtctaagtttagcacta720aagaggcagttgggaaagtgtgcacagttctaaaaagcaaaggatatagcatgaactctg780ttgaaattggcaaggctaaacaatatgcagatatactaaaggcttgtagtccgaaagcta840aaggacttgctgcaatggaccactacaaagaagggcttacatccatttacagcatgttta900atgctactattgattttgggaaaaatgattctatttaaaagagatttgagcttggtttgc960tttgtgttgtgttaaatgcatttaaataaataaaaataaaaataataaaaataaaaaata1020aaataaaaaataaaataattaattattgtttgagtgtattaagtgtgcttttataaatta1080tttgaaataagtttaatgtgtgttttaatatataaacttgaaagtgtgtttgcgtgtgaa1140ataaacaagtaaaaaagctacaaaaaataataaaaacaataaaaaccactacaaaatata1200tatatatatatatataaataaaataaaaaacaaaaaacaaaaaacaaaataaaaaacaaa1260taaaacaaaacaaaaacaaaaaacaaaaaacaaaaacaaaaacaaatacaaatttttggc1320caatttgtgagtagattggatttaaagtttataatttactaatacattcttttaaagcat1380tattaaagcaaccaaattgtgcccgaaggccatccttttttggtttttatttattttatg1440attttttttgtattttttatatattttatgtttatttatttatttcatatgattgttttt1500ggtttatctgttattagatttgtttaatctgatctaaatgcatgtaaacaaactttactt1560agggacaagtttaaccaaggttaatttaaatctaatttagaaatagctttcaaaacatcc1620tctgatctaggagaaatttttatttcacctgcagcatagtcaaatttccaatcgggcatc1680tggatgctatccaaataagacattgtttgtgagtttctcggaagacttttcaaggtctga1740gaaaggaaatattttctcccataaccatcttctgaataatttaaagtctttttccctagc1800tcaaacttttttggctttagatctggctcatttatagtgaagacaatcatgtcttgctga1860acactgtcacagacagtttgagcaagttctgtgtggatttcaagaagtgaatgaacaatt1920ctttgaggatacgacatatcatggataacattgtaaggttcttttagatcagaaaaattc1980atctcaattttatgaatcactgctagaaggagttgattcaagaagaggtttgactgatca2040aagttatattcaatgactgtttcaacattgttgtgattcggaagacattgaatgcttata2100taatactctttaggagaagattcatcagacagggtgaagaaccggctgtatgtgtttgat2160ttcattattttagggatgcgcatccaaaggctgattttaaaaggcttgttattattgagc2220ctgacaaacgaatgagtatctgacattaaatcagaagttgcagacattgccagtgatttt2280acagccaaagtgtttactgtcctgttgttggtctgggattggttaaaaacaggcttaagt2340gtgtacagccattcatgaacacttctggtaggagaaagaacactgactttgcccatggat2400tggttgtttgaatacttgacatcaaaaaggacttctttgacacaagacaatgatccttta2460tttgcagcttcaatgaaattatttggagggaagatgtcagattctttcagaccgaatctt2520gaagtaatttcaggcactgctatttgctcttcaagcattctgagcaactgagaggataac2580actttgagatgaccttgatgtttaacaccgtttgtgtttgttgtgttagatctgaccaga2640atttcgacaacattccctgaaaaggagaaattcagatcaatgtcatcaaaaataggcaca2700aaacatctcactataatttctttttcttctgttgggagaaagccaactttaccagtgtaa2760cagaaactggtcttgctcctggagtcagaatagagttgagcttcgaccaacttctttccg2820gaagattgatcatgaatccaataactatctactactgctttacccgaagatgttgttccc2880cagattgaggacttcgatttgataattgtttcatacattgcactagacatgttaaaatgg2940aagtagtaatgtaattgacaatattgtaagatttgttgtagcttggttgttcattgctct3000 ( 2 ) information for seq id no : 10 :( i ) sequence characteristics :( a ) length : 2993 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 10 : agagcaattgtgtcacgaatataacatcatataatccaaagctagaaactgaaaaattac60aaattttaccaaatactactttaaccgcaagtactatttagtaatttgaacactttaagc120tttaaagttagtaaagtcagcagatcaagatgaacaaagcaaagattaccaaggagaaca180tagtcaagcttttgactcaatctgattccttagaatttgaggaaactcaaaatgaagggt240ctttcaatttcactgacttctttaccaacaaccgtgaaaagattcagaacatgactactg300catcctgcctatccttcctcaagaataggcaatctataatgagagtcattaagagtgctg360acttcacttttggatcagtcacaattaagaaaactagaaacaattcagaaagagttgggg420tcaatgatatgactttccgaagacttgatgcaatggtgagagttcatcttgttggaatga480taaaggacaatggatctgctctgactgaagctataaatagccttccaagtcaccctctga540ttgcctcatatggtcttgcaaccacagatttgaaatcctgtgtcttgggtgttctcttag600gaggaagtcttcctctgatagcatctgtactgaattttgaaatagctgcactagttccgg660ctatttatcaagatgctaaacatgttgagcttggaattgacatgtctaagtttagcacta720aagaggcagttgggaaagtgtgcacagttctaaaaagcaaaggatatagcatgaactctg780ttgaaattggcaaggctaaacaatatgcagatatactaaaggcttgtagtccgaaagcta840aaggacttgctgcaatggaccactacaaagaagggcttacatccatttacagcatgttta900atgctactattgattttgggaaaaatgattctatttaaaagagatttgagcttggtttgc960tttgtgttgtgttaaatgcatttaaataaataaaaataaaaataataaaaataaaaaata1020aaataaaaaataaaataattaattattgtttgagtgtattaagtgtgcttttataaatta1080tttgaaataagtttaatgtgtgttttaatatataaacttgaaagtgtgtttgcgtgtgaa1140ataaacaagtaaaaaagctacaaaaaataataaaaacaataaaaaccactacaaaatata1200tatatatatatatataaataaaataaaaaacaaaaaacaaaaaacaaaataaaaaacaaa1260taaaacaaaacaaaaacaaaaaacaaaaaacaaaaacaaaaacaaatacaaatttttggc1320caatttgtgagtagattggatttaaagtttataatttactaatacattcattttaaagca1380ttattaaagcaaccaaattgtgcccgaaggccatccttttttggtttttatttattttat1440gattttttttgtattttttatatattttatgtttatttatttatttcatatgattgtttt1500tggtttatctgttattagatttgtttaatctgatctaaatgcatgtaaacaaactttact1560tagggacaagtttaaccaaggttaatttaaatctaatttagaaatagctttcaaaacatc1620ctctgatctaggagaaatttttatttcacctgcagcatagtcaaatttccaatcgggcat1680ctggatgctatccaaataagacattgtttgtgagtttctcggaagacttttcaaggtctg1740agaaaggaaatattttctcccataaccatcttctgaataatttaaagtctttttccctag1800ctcaaacttttttggctttagatctggctcatttatagtgaagacaatcatgtcttgctg1860aacactgtcacagacagtttgagcaagttctgtgtggatttcaagaagtgaatgaacaat1920tctttgaggatacgacatatcatggataacattgtaaggttcttttagatcagaaaaatt1980catctcaattttatgaatcactgctagaaggagttgattcaagaagaggtttgactgatc2040aaagttatattcaatgactgtttcaacattgttgtgattcggaagacattgaatgcttat2100ataatactctttaggagaagattcatcagacagggtgaagaaccggctgtatgtgtttga2160tttcattattttagggatgcgcatccaaaggctgattttaaaaggcttgttattattgag2220cctgacaaacgaatgagtatctgacattaaatcagaagttgcagacattgccagtgattt2280tacagccaaagtgtttactgtcctgttgttggtctgggattggttaaaaacaggcttaag2340tgtgtacagccattcatgaacacttctggtaggagaaagaacactgactttgcccatgga2400ttggttgtttgaatacttgacatcaaaaaggacttctttgacacaagacaatgatccttt2460atttgcagcttcaatgaaattatttggagggaagatgtcagattctttcagaccgaatct2520tgaagtaatttcaggcactgctatttgctcttcaagcattctgagcaactgagaggataa2580cactttgagatgaccttgatgtttaacaccgtttgtgtttgttgtgttagatctgaccag2640aatttcgacaacattccctgaaaaggagaaattcagatcaatgtcatcaaaaataggcac2700aaaacatctcactataatttctttttcttctgttgggagaaagccaactttaccagtgta2760acagaaactggtcttgctcctggagtcagaatagagttgagcttcgaccaacttctttcc2820ggaagattgatcatgaatccaataactatctactactgctttacccgaagatgttgttcc2880ccagattgaggacttcgatttgataattgtttcatacattgcactagacatgttaaaatg2940gaagtagtaatgtaattgacaatattgtaagatttgttgtagcttggttgttc2993 ( 2 ) information for seq id no : 11 :( i ) sequence characteristics :( a ) length : 789 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 11 : atgaacaaagcaaagattaccaaggagaacatagtcaagcttttgactcaatctgattcc60ttagaatttgaggaaactcaaaatgaagggtctttcaatttcactgacttctttaccaac120aaccgtgaaaagattcagaacatgactactgcatcctgcctatccttcctcaagaatagg180caatctataatgagagtcattaagagtgctgacttcacttttggatcagtcacaattaag240aaaactagaaacaattcagaaagagttggggtcaatgatatgactttccgaagacttgat300gcaatggtgagagttcatcttgttggaatgataaaggacaatggatctgctctgactgaa360gctataaatagccttccaagtcaccctctgattgcctcatatggtcttgcaaccacagat420ttgaaatcctgtgtcttgggtgttctcttaggaggaagtcttcctctgatagcatctgta480ctgaattttgaaatagctgcactagttccggctatttatcaagatgctaaacatgttgag540cttggaattgacatgtctaagtttagcactaaagaggcagttgggaaagtgtgcacagtt600ctaaaaagcaaaggatatagcatgaactctgttgaaattggcaaggctaaacaatatgca660gatatactaaaggcttgtagtccgaaagctaaaggacttgctgcaatggaccactacaaa720gaagggcttacatccatttacagcatgtttaatgctactattgattttgggaaaaatgat780tctatttaa789 ( 2 ) information for seq id no : 12 :( i ) sequence characteristics :( a ) length : 1350 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 12 : ttaatttaaatctaatttagaaatagctttcaaaacatcctctgatctaggagaaatttt60tatttcacctgcagcatagtcaaatttccaatcgggcatctggatgctatccaaataaga120cattgtttgtgagtttctcggaagacttttcaaggtctgagaaaggaaatattttctccc180ataaccatcttctgaataatttaaagtctttttccctagctcaaacttttttggctttag240atctggctcatttatagtgaagacaatcatgtcttgctgaacactgtcacagacagtttg300agcaagttctgtgtggatttcaagaagtgaatgaacaattctttgaggatacgacatatc360atggataacattgtaaggttcttttagatcagaaaaattcatctcaattttatgaatcac420tgctagaaggagttgattcaagaagaggtttgactgatcaaagttatattcaatgactgt480ttcaacattgttgtgattcggaagacattgaatgcttatataatactctttaggagaaga540ttcatcagacagggtgaagaaccggctgtatgtgtttgatttcattattttagggatgcg600catccaaaggctgattttaaaaggcttgttattattgagcctgacaaacgaatgagtatc660tgacattaaatcagaagttgcagacattgccagtgattttacagccaaagtgtttactgt720cctgttgttggtctgggattggttaaaaacaggcttaagtgtgtacagccattcatgaac780acttctggtaggagaaagaacactgactttgcccatggattggttgtttgaatacttgac840atcaaaaaggacttctttgacacaagacaatgatcctttatttgcagcttcaatgaaatt900atttggagggaagatgtcagattctttcagaccgaatcttgaagtaatttcaggcactgc960tatttgctcttcaagcattctgagcaactgagaggataacactttgagatgaccttgatg1020tttaacaccgtttgtgtttgttgtgttagatctgaccagaatttcgacaacattccctga1080aaaggagaaattcagatcaatgtcatcaaaaataggcacaaaacatctcactataatttc1140tttttcttctgttgggagaaagccaactttaccagtgtaacagaaactggtcttgctcct1200ggagtcagaatagagttgagcttcgaccaacttctttccggaagattgatcatgaatcca1260ataactatctactactgctttacccgaagatgttgttccccagattgaggacttcgattt1320gataattgtttcatacattgcactagacat1350 ( 2 ) information for seq id no : 13 :( i ) sequence characteristics :( a ) length : 642 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 13 : aagagatttgagcttggtttgctttgtgttgtgttaaatgcatttaaataaataaaaata60aaaataataaaaataaaaaataaaataaaaaataaaataattaattattgtttgagtgta120ttaagtgtgcttttataaattatttgaaataagtttaatgtgtgttttaatatataaact180tgaaagtgtgtttgcgtgtgaaataaacaagtaaaaaagctacaaaaaataataaaaaca240ataaaaaccactacaaaatatatatatatatatatataaataaaataaaaaacaaaaaac300aaaaaacaaaataaaaaacaaataaaacaaaacaaaaacaaaaaacaaaaaacaaaaaca360aaaacaaatacaaatttttggccaatttgtgagtagattggatttaaagtttataattta420ctaatacattcttttaaagcattattaaagcaaccaaattgtgcccgaaggccatccttt480tttggtttttatttattttatgattttttttgtattttttatatattttatgtttattta540tttatttcatatgattgtttttggtttatctgttattagatttgtttaatctgatctaaa600tgcatgtaaacaaactttacttagggacaagtttaaccaagg642 ( 2 ) information for seq id no : 14 :( i ) sequence characteristics :( a ) length : 4970 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 14 : agagcaatcagtgcatcaaaattatatctagccgaattcaatcattatcttctcaatatt60ttaattcttaatctaccgtccagagatgaatagttttttcaaatcactcagatcatctag120cagcagggagctagatcaccctagggttacaactaccctctctaaacaaggagcagacat180tgttgtacacaatccttctgctaatcacaacaacaaggaagttctccaaagagccatgga240tagctctaaagggaagattttgatgaacaatacaggcacctcatcactaggcacatatga300gtctgaccagatatctgaatcagagtcttatgatctttctgctagaatgattgttgatac360aaatcatcatatctccagctggaaaaatgatctttttgtaggtaatggtgataaagctgc420aaccaagataattaagatacatccaacctgggatagcagaaaacaatacatgatgatctc480aaggatagttatctggatatgccctactatagctgatcctgatgggaaattggctgtagc540tttaattgatcctaacaagagtgttaatgccagaactgttttgaaagggcaaggaagcat600taaagatcctatatgttttgttttttatctaaattggtccattccaaaagttaacaacac660ttcagagaattgtgttcagcttcatttattatgtgatcaagtttacaagaaagatgtttc720ttttgctagtgtcatgtattcttggacaaaagaattctgtgattcaccaagagcagatct780ggataaaagctgcatgataatacccatcaatagggctattagagccaaatcgcaagcctt840cattgaagcctgcaagttaatcatacctaaaggcaattctgaaaagcaaattagaagaca900acttgcagagctaagtgctaatttagagaaatctgttgaagaagaggagaatgttactga960taacaagatagagatatcatttgataatgaaatctaaatatgttttcatttaataataaa1020taatatatattgttcataatattttgaatgtttaagtaaaaaataaagcaagataaaaaa1080ctatatatatatatatatatagaagtataaaatatatatgtatttgtgtttaaaaacaaa1140tcaaaaaccaaaaaagaaaaaagaaaaaataaacaaaaaacaaaaacaaaaacaaaaaca1200aacaaaaagcaaaaaatagaaaaaagttgaaaaaaaccaaaaaaattttttttgtaaata1260aataaggctccggccagatttggtctaagacctttttatttgtttttatacattttattt1320gtttttgttgatttttatttttattatttttatattttttatatagtttgcttatttaac1380acttatttagacaaattaaatttatttgattacaatcattctgccttatttaatttaaaa1440cacatttggtgtatattccaatgaatttaatcatataccgctgaagtctagaggaggtct1500tcttctagtgatggtgtctttaccagaagacgtggaaaccaaagaataatcattagtgtc1560ttcaatatattttgtcttgtaagacttgtttctaacatagcctctacacattgtggcaac1620aatagagcagaggtaagcaagagcaaatacaaagagtatgagcaatactactctgactgt1680atcaaagaaggatccaaagtggcttgctataaagttaaaagggcttttaacatagtccca1740aaagctccaaactgatgtgtcagaattatattgctgttcctcgtgtgcatgttggtcatt1800ttgatcaattatgttttctggttccagcacagcaacagaatctacaagtgcctcaactga1860gtatgatttgtctccttctggttctataatcattttttgtttttctgggttagaagtgca1920gaacattgtcaagttatacttattagcacctttctttactgctatctggtatgttgacaa1980tgaacattgtttcatggttaaccttgcagaaaaagttatgtctgatataaatgaggcagc2040acacctcagcccttggctacataagaaacatcccttacagcttaaagagacagaactcaa2100tataggcttttttggtacagttttaaacaattcagaaggtagatccaaaacaattttaag2160cttacctagactaaagatcttttccatataaaaactattctggtcagtaaactgaactgg2220aatgtccgatatttggttcaaacctgttttaaatctgtatgtgtcataaccacatgattt2280tatcgtaattgtttttttaccaattgctgaacaatcccaggacagatcgtttgtatctaa2340tgttttcttagagaaaatgggatcaccttggtgtgaaagttgaggatgaccaaacatttt2400tgatggattatttaatctagctatgtttcccgcatatacgtgactatcaggtccatgagc2460tatcagctggcctattgttaagccatcattatggaaatccgctaatatatcagcctggaa2520atatcctgattcagatgggacttcctcagatacagtgaaacactttgctcccacaaatcc2580agatatacatacttcagacttgattgttgatttaataacagaataaatcctgaaagattg2640atccatatcatacacatttctacaaaacccacaagtggctccttcattgatagccaaaca2700ccaaacctcttcacaaccccagtaagatgttggtgttatgcagaaatcttgatacccagt2760tatcggttgttcttttctgcaatctgagcatttacctgtgcatgttgaaaagaaatcagt2820gtgggtgctttgtataggagctgtagtgtattgttcagaaacatcatactgtattctaac2880ttttttaatataaacaacaaacttctgagcagtgctagaacttttgtcattaagagagaa2940aactgtgcccccacctgataataaagattcttctatcatgtatctatattttccatctat3000caccgagtcaaatatgagagattttcttggaaaaatgctttcaggtatgtctgattcatt3060agatttaagtgcatctccagaaatgtatccatatttttcagttttattgtagaaatcaat3120tataccattcctaagccttttcatgaagtgtagattcacagcattcaatcccaatgtgtc3180accagaatattctaagaacccattatctaaaggcttgctttggaaaatagaggcatactc3240acaaccaaatctgcatttgacaaaagttactaaagcattttcagttatcctgcctttgca3300ttcttgataaggtatacaatccataggaccttctgtcacaacattggttagaaagttaga3360ttctacaatagaattttctttaatagcacagaagcattggtctttttcaggacatttgtc3420atatctgtttgtaacaaagcggtcacaaccagggacataataacagctatccaaacactg3480agcagtttgagccatagacataggcatctgtgacaaaatcagaaatcctatcaaagtttc3540tgtgactgcttttaggaaagagaggcctatttttgtattaactatcaaatggaaccattc3600aatgctagcccagttgtattttttattcttctctgctgttctagttattataggacattc3660ttctgagtgttcttcagaggctttgtttttgttacaaatgcataattttgagcattcatg3720ggttaccaaacataaatttccacagaccttacatttcaagggaaaataagaccataaata3780atttatcagtagtagtataggatacgttatcaatcccagaagatcatacccatagaacag3840tgttttagatgttttgtttaccaagtaccttatagggaaatagacaatcagagcaatcat3900gatcaatctaaaccatgagaagttgatgcaagcagtttgtttgtaaatatttttggagta3960ctttataatacaatctctaactcttttgtccactaaaggaactttagaagacttgtcacc4020gcacaataggttatgcttaccatccatattttcttctgtgaaagtcaaactaactgagcc4080agagaagcttattatggaatggctcatgtcacttccttctcttttgacgacgtaacccat4140gattttctcaggtgtagttaatgaaactgtataagaattaactatgtttgtttttgatat4200tttacaatcacctgagaatttcacactctggagagagactgtgccattagttggtctaga4260attgtacatgattggataattgtaattctccaaactttcaattatatagaatttagttcc4320tatagataatttccttttgttatcgatttttgttattggtacaactggaacagtttcaaa4380gcttcttggcaattcagaagatccttcacagtttcccaatttagttatagtgtcactgat4440acatgaatatataacaccattgctttctacttggtaataaacattgaatgttgaaactcc4500tttaatgctacaagtcaaacttgaagcatttaggcatggatttggtaaatccataactga4560tatagttgttggtgtagaagacaatccacttggagattgaggtacctcattattggcaag4620aacagtttgagtatctcgtgttggtctaagggttttacctgttgcattctggagcatttc4680agccaaagtatctagaatttcatttttatgatctacagaacggtcataataagcttcatc4740ataaatttctggatgatcgcccctttcaacatgaatctttgcatctgtctcctttaatgc4800cataaaggataagataacagaagtaacaactagtgtacatacactaattttaacaagtaa4860ctcgcacatctttagaattttcattctaaaaagtcgaataacactagttctaaaattgct4920ttatgagtttgatctgttgtatgtagagttttgtttgcactgattgctct4970 ( 2 ) information for seq id no : 15 :( i ) sequence characteristics :( a ) length : 912 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 15 : atgaatagttttttcaaatcactcagatcatctagcagcagggagctagatcaccctagg60gttacaactaccctctctaaacaaggagcagacattgttgtacacaatccttctgctaat120cacaacaacaaggaagttctccaaagagccatggatagctctaaagggaagattttgatg180aacaatacaggcacctcatcactaggcacatatgagtctgaccagatatctgaatcagag240tcttatgatctttctgctagaatgattgttgatacaaatcatcatatctccagctggaaa300aatgatctttttgtaggtaatggtgataaagctgcaaccaagataattaagatacatcca360acctgggatagcagaaaacaatacatgatgatctcaaggatagttatctggatatgccct420actatagctgatcctgatgggaaattggctgtagctttaattgatcctaacaagagtgtt480aatgccagaactgttttgaaagggcaaggaagcattaaagatcctatatgttttgttttt540tatctaaattggtccattccaaaagttaacaacacttcagagaattgtgttcagcttcat600ttattatgtgatcaagtttacaagaaagatgtttcttttgctagtgtcatgtattcttgg660acaaaagaattctgtgattcaccaagagcagatctggataaaagctgcatgataataccc720atcaatagggctattagagccaaatcgcaagccttcattgaagcctgcaagttaatcata780cctaaaggcaattctgaaaagcaaattagaagacaacttgcagagctaagtgctaattta840gagaaatctgttgaagaagaggagaatgttactgataacaagatagagatatcatttgat900aatgaaatctaa912 ( 2 ) information for seq id no : 16 :( i ) sequence characteristics :( a ) length : 473 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 16 : atatgttttcatttaataataaataatatatattgttcataatattttgaatgtttaagt60aaaaaataaagcaagataaaaaactatatatatatatatatatagaagtataaaatatat120atgtatttgtgtttaaaaacaaatcaaaaaccaaaaaagaaaaaagaaaaaataaacaaa180aaacaaaaacaaaaacaaaaacaaacaaaaagcaaaaaatagaaaaaagttgaaaaaaac240caaaaaaattttttttgtaaataaataaggctccggccagatttggtctaagaccttttt300atttgtttttatacattttatttgtttttgttgatttttatttttattatttttatattt360tttatatagtttgcttatttaacacttatttagacaaattaaatttatttgattacaatc420attctgccttatttaatttaaaacacatttggtgtatattccaatgaatttaa473 ( 2 ) information for seq id no : 17 :( i ) sequence characteristics :( a ) length : 3414 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 17 : tcatataccgctgaagtctagaggaggtcttcttctagtgatggtgtctttaccagaaga60cgtggaaaccaaagaataatcattagtgtcttcaatatattttgtcttgtaagacttgtt120tctaacatagcctctacacattgtggcaacaatagagcagaggtaagcaagagcaaatac180aaagagtatgagcaatactactctgactgtatcaaagaaggatccaaagtggcttgctat240aaagttaaaagggcttttaacatagtcccaaaagctccaaactgatgtgtcagaattata300ttgctgttcctcgtgtgcatgttggtcattttgatcaattatgttttctggttccagcac360agcaacagaatctacaagtgcctcaactgagtatgatttgtctccttctggttctataat420cattttttgtttttctgggttagaagtgcagaacattgtcaagttatacttattagcacc480tttctttactgctatctggtatgttgacaatgaacattgtttcatggttaaccttgcaga540aaaagttatgtctgatataaatgaggcagcacacctcagcccttggctacataagaaaca600tcccttacagcttaaagagacagaactcaatataggcttttttggtacagttttaaacaa660ttcagaaggtagatccaaaacaattttaagcttacctagactaaagatcttttccatata720aaaactattctggtcagtaaactgaactggaatgtccgatatttggttcaaacctgtttt780aaatctgtatgtgtcataaccacatgattttatcgtaattgtttttttaccaattgctga840acaatcccaggacagatcgtttgtatctaatgttttcttagagaaaatgggatcaccttg900gtgtgaaagttgaggatgaccaaacatttttgatggattatttaatctagctatgtttcc960cgcatatacgtgactatcaggtccatgagctatcagctggcctattgttaagccatcatt1020atggaaatccgctaatatatcagcctggaaatatcctgattcagatgggacttcctcaga1080tacagtgaaacactttgctcccacaaatccagatatacatacttcagacttgattgttga1140tttaataacagaataaatcctgaaagattgatccatatcatacacatttctacaaaaccc1200acaagtggctccttcattgatagccaaacaccaaacctcttcacaaccccagtaagatgt1260tggtgttatgcagaaatcttgatacccagttatcggttgttcttttctgcaatctgagca1320tttacctgtgcatgttgaaaagaaatcagtgtgggtgctttgtataggagctgtagtgta1380ttgttcagaaacatcatactgtattctaacttttttaatataaacaacaaacttctgagc1440agtgctagaacttttgtcattaagagagaaaactgtgcccccacctgataataaagattc1500ttctatcatgtatctatattttccatctatcaccgagtcaaatatgagagattttcttgg1560aaaaatgctttcaggtatgtctgattcattagatttaagtgcatctccagaaatgtatcc1620atatttttcagttttattgtagaaatcaattataccattcctaagccttttcatgaagtg1680tagattcacagcattcaatcccaatgtgtcaccagaatattctaagaacccattatctaa1740aggcttgctttggaaaatagaggcatactcacaaccaaatctgcatttgacaaaagttac1800taaagcattttcagttatcctgcctttgcattcttgataaggtatacaatccataggacc1860ttctgtcacaacattggttagaaagttagattctacaatagaattttctttaatagcaca1920gaagcattggtctttttcaggacatttgtcatatctgtttgtaacaaagcggtcacaacc1980agggacataataacagctatccaaacactgagcagtttgagccatagacataggcatctg2040tgacaaaatcagaaatcctatcaaagtttctgtgactgcttttaggaaagagaggcctat2100ttttgtattaactatcaaatggaaccattcaatgctagcccagttgtattttttattctt2160ctctgctgttctagttattataggacattcttctgagtgttcttcagaggctttgttttt2220gttacaaatgcataattttgagcattcatgggttaccaaacataaatttccacagacctt2280acatttcaagggaaaataagaccataaataatttatcagtagtagtataggatacgttat2340caatcccagaagatcatacccatagaacagtgttttagatgttttgtttaccaagtacct2400tatagggaaatagacaatcagagcaatcatgatcaatctaaaccatgagaagttgatgca2460agcagtttgtttgtaaatatttttggagtactttataatacaatctctaactcttttgtc2520cactaaaggaactttagaagacttgtcaccgcacaataggttatgcttaccatccatatt2580ttcttctgtgaaagtcaaactaactgagccagagaagcttattatggaatggctcatgtc2640acttccttctcttttgacgacgtaacccatgattttctcaggtgtagttaatgaaactgt2700ataagaattaactatgtttgtttttgatattttacaatcacctgagaatttcacactctg2760gagagagactgtgccattagttggtctagaattgtacatgattggataattgtaattctc2820caaactttcaattatatagaatttagttcctatagataatttccttttgttatcgatttt2880tgttattggtacaactggaacagtttcaaagcttcttggcaattcagaagatccttcaca2940gtttcccaatttagttatagtgtcactgatacatgaatatataacaccattgctttctac3000ttggtaataaacattgaatgttgaaactcctttaatgctacaagtcaaacttgaagcatt3060taggcatggatttggtaaatccataactgatatagttgttggtgtagaagacaatccact3120tggagattgaggtacctcattattggcaagaacagtttgagtatctcgtgttggtctaag3180ggttttacctgttgcattctggagcatttcagccaaagtatctagaatttcatttttatg3240atctacagaacggtcataataagcttcatcataaatttctggatgatcgcccctttcaac3300atgaatctttgcatctgtctcctttaatgccataaaggataagataacagaagtaacaac3360tagtgtacatacactaattttaacaagtaactcgcacatctttagaattttcat3414 ( 2 ) information for seq id no : 18 :( i ) sequence characteristics :( a ) length : 36 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 18 : agagcaatcagtgcatcaaaattatatctagccgaa36 ( 2 ) information for seq id no : 19 :( i ) sequence characteristics :( a ) length : 36 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 19 : ctgttgtatgtagagttttgtttgcactgattgctc36 ( 2 ) information for seq id no : 20 :( i ) sequence characteristics :( a ) length : 4970 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 20 : agagcaatcagtgcaaacaaaactctacatacaacagatcaaactcataaagcaatttta60gaactagtgttattcgactttttagaatgaaaattctaaagatgtgcgagttacttgtta120aaattagtgtatgtacactagttgttacttctgttatcttatcctttatggcattaaagg180agacagatgcaaagattcatgttgaaaggggcgatcatccagaaatttatgatgaagctt240attatgaccgttctgtagatcataaaaatgaaattctagatactttggctgaaatgctcc300agaatgcaacaggtaaaacccttagaccaacacgagatactcaaactgttcttgccaata360atgaggtacctcaatctccaagtggattgtcttctacaccaacaactatatcagttatgg420atttaccaaatccatgcctaaatgcttcaagtttgacttgtagcattaaaggagtttcaa480cattcaatgtttattaccaagtagaaagcaatggtgttatatattcatgtatcagtgaca540ctataactaaattgggaaactgtgaaggatcttctgaattgccaagaagctttgaaactg600ttccagttgtaccaataacaaaaatcgataacaaaaggaaattatctataggaactaaat660tctatataattgaaagtttggagaattacaattatccaatcatgtacaattctagaccaa720ctaatggcacagtctctctccagagtgtgaaattctcaggtgattgtaaaatatcaaaaa780caaacatagttaattcttatacagtttcattaactacacctgagaaaatcatgggttacg840tcgtcaaaagagaaggaagtgacatgagccattccataataagcttctctggctcagtta900gtttgactttcacagaagaaaatatggatggtaagcataacctattgtgcggtgacaagt960cttctaaagttcctttagtggacaaaagagttagagattgtattataaagtactccaaaa1020atatttacaaacaaactgcttgcatcaacttctcatggtttagattgatcatgattgctc1080tgattgtctatttccctataaggtacttggtaaacaaaacatctaaaacactgttctatg1140ggtatgatcttctgggattgataacgtatcctatactactactgataaattatttatggt1200cttattttcccttgaaatgtaaggtctgtggaaatttatgtttggtaacccatgaatgct1260caaaattatgcatttgtaacaaaaacaaagcctctgaagaacactcagaagaatgtccta1320taataactagaacagcagagaagaataaaaaatacaactgggctagcattgaatggttcc1380atttgatagttaatacaaaaataggcctctctttcctaaaagcagtcacagaaactttga1440taggatttctgattttgtcacagatgcctatgtctatggctcaaactgctcagtgtttgg1500atagctgttattatgtccctggttgtgaccgctttgttacaaacagatatgacaaatgtc1560ctgaaaaagaccaatgcttctgtgctattaaagaaaattctattgtagaatctaactttc1620taaccaatgttgtgacagaaggtcctatggattgtataccttatcaagaatgcaaaggca1680ggataactgaaaatgctttagtaacttttgtcaaatgcagatttggttgtgagtatgcct1740ctattttccaaagcaagcctttagataatgggttcttagaatattctggtgacacattgg1800gattgaatgctgtgaatctacacttcatgaaaaggcttaggaatggtataattgatttct1860acaataaaactgaaaaatatggatacatttctggagatgcacttaaatctaatgaatcag1920acatacctgaaagcatttttccaagaaaatctctcatatttgactcggtgatagatggaa1980aatatagatacatgatagaagaatctttattatcaggtgggggcacagttttctctctta2040atgacaaaagttctagcactgctcagaagtttgttgtttatattaaaaaagttagaatac2100agtatgatgtttctgaacaatacactacagctcctatacaaagcacccacactgatttct2160tttcaacatgcacaggtaaatgctcagattgcagaaaagaacaaccgataactgggtatc2220aagatttctgcataacaccaacatcttactggggttgtgaagaggtttggtgtttggcta2280tcaatgaaggagccacttgtgggttttgtagaaatgtgtatgatatggatcaatctttca2340ggatttattctgttattaaatcaacaatcaagtctgaagtatgtatatctggatttgtgg2400gagcaaagtgtttcactgtatctgaggaagtcccatctgaatcaggatatttccaggctg2460atatattagcggatttccataatgatggcttaacaataggccagctgatagctcatggac2520ctgatagtcacgtatatgcgggaaacatagctagattaaataatccatcaaaaatgtttg2580gtcatcctcaactttcacaccaaggtgatcccattttctctaagaaaacattagatacaa2640acgatctgtcctgggattgttcagcaattggtaaaaaaacaattacgataaaatcatgtg2700gttatgacacatacagatttaaaacaggtttgaaccaaatatcggacattccagttcagt2760ttactgaccagaatagtttttatatggaaaagatctttagtctaggtaagcttaaaattg2820ttttggatctaccttctgaattgtttaaaactgtaccaaaaaagcctatattgagttctg2880tctctttaagctgtaagggatgtttcttatgtagccaagggctgaggtgtgctgcctcat2940ttatatcagacataactttttctgcaaggttaaccatgaaacaatgttcattgtcaacat3000accagatagcagtaaagaaaggtgctaataagtataacttgacaatgttctgcacttcta3060acccagaaaaacaaaaaatgattatagaaccagaaggagacaaatcatactcagttgagg3120cacttgtagattctgttgctgtgctggaaccagaaaacataattgatcaaaatgaccaac3180atgcacacgaggaacagcaatataattctgacacatcagtttggagcttttgggactatg3240ttaaaagcccttttaactttatagcaagccactttggatccttctttgatacagtcagag3300tagtattgctcatactctttgtatttgctcttgcttacctctgctctattgttgccacaa3360tgtgtagaggctatgttagaaacaagtcttacaagacaaaatatattgaagacactaatg3420attattctttggtttccacgtcttctggtaaagacaccatcactagaagaagacctcctc3480tagacttcagcggtatatgattaaattcattggaatatacaccaaatgtgttttaaatta3540aataaggcagaatgattgtaatcaaataaatttaatttgtctaaataagtgttaaataag3600caaactatataaaaaatataaaaataataaaaataaaaatcaacaaaaacaaataaaatg3660tataaaaacaaataaaaaggtcttagaccaaatctggccggagccttatttatttacaaa3720aaaaatttttttggtttttttcaacttttttctattttttgctttttgtttgtttttgtt3780tttgtttttgttttttgtttattttttcttttttcttttttggtttttgatttgttttta3840aacacaaatacatatatattttatacttctatatatatatatatatatagttttttatct3900tgctttattttttacttaaacattcaaaatattatgaacaatatatattatttattatta3960aatgaaaacatatttagatttcattatcaaatgatatctctatcttgttatcagtaacat4020tctcctcttcttcaacagatttctctaaattagcacttagctctgcaagttgtcttctaa4080tttgcttttcagaattgcctttaggtatgattaacttgcaggcttcaatgaaggcttgcg4140atttggctctaatagccctattgatgggtattatcatgcagcttttatccagatctgctc4200ttggtgaatcacagaattcttttgtccaagaatacatgacactagcaaaagaaacatctt4260tcttgtaaacttgatcacataataaatgaagctgaacacaattctctgaagtgttgttaa4320cttttggaatggaccaatttagataaaaaacaaaacatataggatctttaatgcttcctt4380gccctttcaaaacagttctggcattaacactcttgttaggatcaattaaagctacagcca4440atttcccatcaggatcagctatagtagggcatatccagataactatccttgagatcatca4500tgtattgttttctgctatcccaggttggatgtatcttaattatcttggttgcagctttat4560caccattacctacaaaaagatcatttttccagctggagatatgatgatttgtatcaacaa4620tcattctagcagaaagatcataagactctgattcagatatctggtcagactcatatgtgc4680ctagtgatgaggtgcctgtattgttcatcaaaatcttccctttagagctatccatggctc4740tttggagaacttccttgttgttgtgattagcagaaggattgtgtacaacaatgtctgctc4800cttgtttagagagggtagttgtaaccctagggtgatctagctccctgctgctagatgatc4860tgagtgatttgaaaaaactattcatctctggacggtagattaagaattaaaatattgaga4920agataatgattgaattcggctagatataattttgatgcactgattgctct4970 ( 2 ) information for seq id no : 21 :( i ) sequence characteristics :( a ) length : 3414 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 21 : atgaaaattctaaagatgtgcgagttacttgttaaaattagtgtatgtacactagttgtt60acttctgttatcttatcctttatggcattaaaggagacagatgcaaagattcatgttgaa120aggggcgatcatccagaaatttatgatgaagcttattatgaccgttctgtagatcataaa180aatgaaattctagatactttggctgaaatgctccagaatgcaacaggtaaaacccttaga240ccaacacgagatactcaaactgttcttgccaataatgaggtacctcaatctccaagtgga300ttgtcttctacaccaacaactatatcagttatggatttaccaaatccatgcctaaatgct360tcaagtttgacttgtagcattaaaggagtttcaacattcaatgtttattaccaagtagaa420agcaatggtgttatatattcatgtatcagtgacactataactaaattgggaaactgtgaa480ggatcttctgaattgccaagaagctttgaaactgttccagttgtaccaataacaaaaatc540gataacaaaaggaaattatctataggaactaaattctatataattgaaagtttggagaat600tacaattatccaatcatgtacaattctagaccaactaatggcacagtctctctccagagt660gtgaaattctcaggtgattgtaaaatatcaaaaacaaacatagttaattcttatacagtt720tcattaactacacctgagaaaatcatgggttacgtcgtcaaaagagaaggaagtgacatg780agccattccataataagcttctctggctcagttagtttgactttcacagaagaaaatatg840gatggtaagcataacctattgtgcggtgacaagtcttctaaagttcctttagtggacaaa900agagttagagattgtattataaagtactccaaaaatatttacaaacaaactgcttgcatc960aacttctcatggtttagattgatcatgattgctctgattgtctatttccctataaggtac1020ttggtaaacaaaacatctaaaacactgttctatgggtatgatcttctgggattgataacg1080tatcctatactactactgataaattatttatggtcttattttcccttgaaatgtaaggtc1140tgtggaaatttatgtttggtaacccatgaatgctcaaaattatgcatttgtaacaaaaac1200aaagcctctgaagaacactcagaagaatgtcctataataactagaacagcagagaagaat1260aaaaaatacaactgggctagcattgaatggttccatttgatagttaatacaaaaataggc1320ctctctttcctaaaagcagtcacagaaactttgataggatttctgattttgtcacagatg1380cctatgtctatggctcaaactgctcagtgtttggatagctgttattatgtccctggttgt1440gaccgctttgttacaaacagatatgacaaatgtcctgaaaaagaccaatgcttctgtgct1500attaaagaaaattctattgtagaatctaactttctaaccaatgttgtgacagaaggtcct1560atggattgtataccttatcaagaatgcaaaggcaggataactgaaaatgctttagtaact1620tttgtcaaatgcagatttggttgtgagtatgcctctattttccaaagcaagcctttagat1680aatgggttcttagaatattctggtgacacattgggattgaatgctgtgaatctacacttc1740atgaaaaggcttaggaatggtataattgatttctacaataaaactgaaaaatatggatac1800atttctggagatgcacttaaatctaatgaatcagacatacctgaaagcatttttccaaga1860aaatctctcatatttgactcggtgatagatggaaaatatagatacatgatagaagaatct1920ttattatcaggtgggggcacagttttctctcttaatgacaaaagttctagcactgctcag1980aagtttgttgtttatattaaaaaagttagaatacagtatgatgtttctgaacaatacact2040acagctcctatacaaagcacccacactgatttcttttcaacatgcacaggtaaatgctca2100gattgcagaaaagaacaaccgataactgggtatcaagatttctgcataacaccaacatct2160tactggggttgtgaagaggtttggtgtttggctatcaatgaaggagccacttgtgggttt2220tgtagaaatgtgtatgatatggatcaatctttcaggatttattctgttattaaatcaaca2280atcaagtctgaagtatgtatatctggatttgtgggagcaaagtgtttcactgtatctgag2340gaagtcccatctgaatcaggatatttccaggctgatatattagcggatttccataatgat2400ggcttaacaataggccagctgatagctcatggacctgatagtcacgtatatgcgggaaac2460atagctagattaaataatccatcaaaaatgtttggtcatcctcaactttcacaccaaggt2520gatcccattttctctaagaaaacattagatacaaacgatctgtcctgggattgttcagca2580attggtaaaaaaacaattacgataaaatcatgtggttatgacacatacagatttaaaaca2640ggtttgaaccaaatatcggacattccagttcagtttactgaccagaatagtttttatatg2700gaaaagatctttagtctaggtaagcttaaaattgttttggatctaccttctgaattgttt2760aaaactgtaccaaaaaagcctatattgagttctgtctctttaagctgtaagggatgtttc2820ttatgtagccaagggctgaggtgtgctgcctcatttatatcagacataactttttctgca2880aggttaaccatgaaacaatgttcattgtcaacataccagatagcagtaaagaaaggtgct2940aataagtataacttgacaatgttctgcacttctaacccagaaaaacaaaaaatgattata3000gaaccagaaggagacaaatcatactcagttgaggcacttgtagattctgttgctgtgctg3060gaaccagaaaacataattgatcaaaatgaccaacatgcacacgaggaacagcaatataat3120tctgacacatcagtttggagcttttgggactatgttaaaagcccttttaactttatagca3180agccactttggatccttctttgatacagtcagagtagtattgctcatactctttgtattt3240gctcttgcttacctctgctctattgttgccacaatgtgtagaggctatgttagaaacaag3300tcttacaagacaaaatatattgaagacactaatgattattctttggtttccacgtcttct3360ggtaaagacaccatcactagaagaagacctcctctagacttcagcggtatatga3414 ( 2 ) information for seq id no : 22 :( i ) sequence characteristics :( a ) length : 912 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 22 : ttagatttcattatcaaatgatatctctatcttgttatcagtaacattctcctcttcttc60aacagatttctctaaattagcacttagctctgcaagttgtcttctaatttgcttttcaga120attgcctttaggtatgattaacttgcaggcttcaatgaaggcttgcgatttggctctaat180agccctattgatgggtattatcatgcagcttttatccagatctgctcttggtgaatcaca240gaattcttttgtccaagaatacatgacactagcaaaagaaacatctttcttgtaaacttg300atcacataataaatgaagctgaacacaattctctgaagtgttgttaacttttggaatgga360ccaatttagataaaaaacaaaacatataggatctttaatgcttccttgccctttcaaaac420agttctggcattaacactcttgttaggatcaattaaagctacagccaatttcccatcagg480atcagctatagtagggcatatccagataactatccttgagatcatcatgtattgttttct540gctatcccaggttggatgtatcttaattatcttggttgcagctttatcaccattacctac600aaaaagatcatttttccagctggagatatgatgatttgtatcaacaatcattctagcaga660aagatcataagactctgattcagatatctggtcagactcatatgtgcctagtgatgaggt720gcctgtattgttcatcaaaatcttccctttagagctatccatggctctttggagaacttc780cttgttgttgtgattagcagaaggattgtgtacaacaatgtctgctccttgtttagagag840ggtagttgtaaccctagggtgatctagctccctgctgctagatgatctgagtgatttgaa900aaaactattcat912 ( 2 ) information for seq id no : 23 :( i ) sequence characteristics :( a ) length : 446 base pairs ( b ) type : nucleic acid ( c ) strandedness : single ( d ) topology : linear ( xi ) sequence description : seq id no : 23 : ggatccggaacatggtggagcacgacacgcttgtctactccaaaaatatcaaagatacag60tctcagaagaccaaagggcaattgagacttttcaacaaagttattgtgaagatagtggaa120aaggaaggtggctcctacaaatgccatcattgcgataaaggaaaggccatcgttgaagat180gcctctgccgacagtggtcccaaagatggacccccacccacgaggagcatcgtggaaaaa240gaagacgttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactgacgta300agggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttca360tttcatttggagaggactttttacaacaattaccaacaacaacaaacaacaaacaacatt420acaattactatttacaattacccggg446 ( 2 ) information for seq id no : 24 :( i ) sequence characteristics :( a ) length : 861 amino acids ( b ) type : amino acid ( c ) strandedness : unknown ( d ) topology : unknown ( ii ) molecule type : protein ( xi ) sequence description : seq id no : 24 : 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( 2 ) information for seq id no : 25 :( i ) sequence characteristics :( a ) length : 744 amino acids ( b ) type : amino acid ( c ) strandedness : unknown ( d ) topology : unknown ( ii ) molecule type : protein ( xi ) sequence description : seq id no : 25 : 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( 2 ) information for seq id no : 26 :( i ) sequence characteristics :( a ) length : 1261 amino acids ( b ) type : amino acid ( c ) strandedness : unknown ( d ) topology : unknown ( ii ) molecule type : protein ( xi ) sequence description : seq id no : 26 : 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( 2 ) information for seq id no : 27 :( i ) sequence characteristics :( a ) length : 3218 amino acids ( b ) type : amino acid ( c ) strandedness : unknown ( d ) topology : unknown ( ii ) molecule type : protein ( xi ) sequence description : seq id no : 27 : 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