Opinion ID: 4682273
Heading Depth: 5
Heading Rank: 3

Heading: a plurality of cells each comprising a

Text: plurality of polynucleotide molecules; (b) generating a plurality of droplets, wherein at least 1,000 droplets of said plurality of droplets each comprise: (i) a single gel bead from said at least 1,000 gel beads; and Case: 20-1785 Document: 80 Page: 7 Filed: 04/29/2021 BIO-RAD LABORATORIES, INC. v. ITC 7 (ii) a single cell from said plurality of cells; and (c) in each of said at least 1,000 droplets, using said plurality of polynucleotide molecules from said single cell and barcode molecules of said at least 1,000 barcode molecules from said single gel bead to generate a plurality of barcoded polynu- cleotide molecules, wherein said barcode molecules become detached from said gel bead. ’530 patent, col. 47, line 58, through col. 49, line 4.