Opinion ID: 4552520
Heading Depth: 3
Heading Rank: 1

Heading: A method of operating a flow cytometry appa-

Text: ratus with at least n detectors to analyze at least two populations of particles in the same sample, the method comprising: (a) establishing a fluid stream in the flow cytometry apparatus with at least n detectors, the at least n detectors including a first detector and a second detector; (b) entraining particles from the sample in the fluid stream in the flow cytometry apparatus; Case: 19-1789 Document: 58 Page: 6 Filed: 07/31/2020 6 XY, LLC v. TRANS OVA GENETICS, LC (c) executing instructions read from a computer readable memory with a processor, the processor being in communication with the first detector in the flow cytometer, to detect a first signal from the first detector based on individual particles in the fluid stream; (d) executing instructions read from the computer readable memory with the processor, the processor being in communication with the second detector in the flow cytometer, to detect a second signal from the second detector based on the individual particles in the fluid stream; (e) executing instructions read from the computer readable memory with the processor to convert at least the first signal and the second signal into n-dimensional parameter data for detected particles in the sample, wherein the n-dimensional pa- rameter data for particles from the at least two populations overlap in at least one of the dimen- sions; (f) executing instructions read from the computer readable memory with the processor to rotationally alter the n-dimensional parameter data so that spatial separation of the data from the particles from the at least two populations in the at least one dimension that is overlapped is increased; (g) executing instructions read from the computer readable memory with the processor to real-time classify each of the individual detected particles into one of a first population and a second popula- tion of the at least two populations based on at least the rotationally altered n-dimensional parameter data; and Case: 19-1789 Document: 58 Page: 7 Filed: 07/31/2020 XY, LLC v. TRANS OVA GENETICS, LC 7 (h) using the real-time classification, sorting the individual particles with the flow cytometer. Id. at col. 16 l. 54 – col. 17 l. 27.