Opinion ID: 6343334
Heading Depth: 3
Heading Rank: 1

Heading: A method of producing a phytase in fungal cells,

Text: the method comprising: providing a polynucleotide encoding an Escherichia coli phytase; expressing the polynucleotide in the fungal cells; and isolating the expressed Escherichia coli phytase wherein the Escherichia coli phytase catalyzes the release of phosphate from phytate. 10. The method of claim 1 wherein the Escherichia coli phytase has an optimum activity at a temper- ature range of 57 degrees C. to 65 degrees C. 11. The method of claim 1 wherein the Escherichia coli phytase retains at least 40% of its activity after heating the phytase for 15 minutes at 80 degrees C. 12. The method of claim 1 wherein the Escherichia coli phytase retains at least 60% of its activity after heating the phytase for 15 minutes at 60 degrees C. Dependent claims 10–12 add so-called “thermostability limitations” to the phytases produced by the heterologous method described in independent claim 1. Case: 20-2334 Document: 67 Page: 4 Filed: 05/24/2022 4 CORNELL RESEARCH FOUNDATION, INC. v. VIDAL