Opinion ID: 4682273
Heading Depth: 3
Heading Rank: 1

Heading: A method for sample preparation, comprising:

Text: a) providing a droplet comprising a porous gel bead and a target nucleic acid analyte, wherein said porous gel bead comprises at least 1,000,000 oligonucleotide molecules comprising barcode sequences, wherein said oligonucleotide molecules are releasably attached to said porous gel bead, wherein said barcode sequences are the same se- quence for said oligonucleotide molecules; b) applying a stimulus to said porous gel bead to release said oligonucleotide molecules from said porous gel bead into said droplet, wherein upon release from said porous gel bead, a given oligonucleotide molecule from said oligonucleotide mole- Case: 20-1785 Document: 80 Page: 5 Filed: 04/29/2021 BIO-RAD LABORATORIES, INC. v. ITC 5 cules attaches to said target nucleic acid analyte; and c) subjecting said given oligonucleotide molecule attached to said target nucleic acid analyte to nucleic acid amplification to yield a barcoded target nucleic acid analyte. Id., col. 33, line 56, through col. 34, line 7. With respect to the ’468 patent, 10X asserted independent claim 1 and dependent claims 6, 7, 9, and 21 against Bio-Rad. Claim 1 recites: 1. A method for droplet generation, comprising: (a) providing at least 1,000,000 oligonucleotide molecules comprising barcode sequences, wherein said barcode sequences are the same sequence for said at least 1,000,000 oligonucleotide molecules, wherein said at least 1,000,000 oligonucleotide molecules are releasably attached to a bead, wherein said bead is porous; (b) combining said at least 1,000,000 oligonucleotide molecules and a sample comprising a nucleic acid analyte each in an aqueous phase at a first junction of two or more channels of a microfluidic device to form an aqueous mixture comprising said at least 1,000,000 oligonucleotide molecules attached to said bead and said sample; and (c) generating a droplet comprising said at least 1,000,000[ ]oligonucleotide molecules attached to said bead and said sample comprising said nucleic acid analyte by contacting said aqueous mixture with an immiscible continuous phase at a second junction of two or more channels of said microflu- idic device. ’468 patent, col. 33, line 56, through col. 34, line 9. Case: 20-1785 Document: 80 Page: 6 Filed: 04/29/2021 6 BIO-RAD LABORATORIES, INC. v. ITC The third patent asserted by 10X here is the ’530 patent, entitled “Methods and Systems for Processing Polynucleotides.” It lists Benjamin Hindson, Serge Saxonov, and Michael Schnall-Levin as three of six inventors. It is undisputed that the conception date for the inventions of this patent is no earlier than the January 2013 date for the ’024 and ’468 patents. Although the ’530 patent does not share a specification with the other two patents at issue here, the subject matter of the asserted claims is related to that of the asserted ’024 and ’468 patent claims. 10X asserted independent claim 1 and dependent claims 4, 11, 14, 19, 26, and 28 of the ’530 patent. Claim 1 recites: 1. A method for nucleic acid preparation or analy- sis, comprising:

least 1,000 gel beads, at least 1,000 bar- code molecules comprising identical bar- code sequences that are distinct from barcode sequences of at least 1,000 bar- code molecules releasably attached to any other gel bead of said at least 1,000 gel beads; and
plurality of polynucleotide molecules; (b) generating a plurality of droplets, wherein at least 1,000 droplets of said plurality of droplets each comprise: (i) a single gel bead from said at least 1,000 gel beads; and Case: 20-1785 Document: 80 Page: 7 Filed: 04/29/2021 BIO-RAD LABORATORIES, INC. v. ITC 7 (ii) a single cell from said plurality of cells; and (c) in each of said at least 1,000 droplets, using said plurality of polynucleotide molecules from said single cell and barcode molecules of said at least 1,000 barcode molecules from said single gel bead to generate a plurality of barcoded polynu- cleotide molecules, wherein said barcode molecules become detached from said gel bead. ’530 patent, col. 47, line 58, through col. 49, line 4.