Opinion ID: 3173494
Heading Depth: 3
Heading Rank: 6

Heading: determining the positions of modified ba-

Text: ses in the sequence of the double-stranded nucleic acid sample by comparing the se- quences of the altered forward and reverse strands. 23. The method of claim 1, wherein: the forward and reverse strands of the circu- lar pair-locked molecule are locked togeth- er by nucleic acid inserts; 8 INDUSTRIAL TECH. RESEARCH INST. v. PAC. BIOSCIENCES the sequence data obtained in step (b) com- prise at least two copies of the sequence of the circular pair-locked molecule, each copy comprising sequences of first and sec- ond insert-sample units; the sequences of the first and second insert- sample units comprise insert sequences, which may be identical or non-identical, and oppositely oriented repeats of the se- quence of the nucleic acid sample; and the method further comprises: