PATENT CLAIM ANALYSIS

Application Number: 16279245
Application Type: Utility
Filing Date: 2019-02
Publication Date: 2019-08
Patent Classification: ["506", "002000"]

Abstract:
This application discloses methods for cDNA synthesis with improved reverse transcription, template switching and preamplification to increase both yield and average length of cDNA libraries generated from individual cells. The new methods include exchanging a single nucleoside residue for a locked nucleic acid (LNA) at the TSO 3′ end, using a methyl group donor, and/or a MgCl 2  concentration higher than conventionally used. Single-cell transcriptome analyses incorporating these differences have full-length coverage, improved sensitivity and accuracy, have less bias and are more amendable to cost-effective automation. The invention also provides cDNA molecules comprising a locked nucleic acid at the 3′-end, compositions and cDNA libraries comprising these cDNA molecules, and methods for single-cell transcriptome profiling.

Claim (Index 47):
The cDNA library of  claim 42 , wherein the method further comprises PCR preamplification, tagmentation, and final PCR amplification.

Metadata:
- Claim Count in Document: 59.0
- Percentile: 99.0
- Lexical Diversity: 1.45977
- Patent Class: 506.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14912556', '15137394', '15240166', '15947971', '15514931']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5828347351975955
- 35 USC 102 Novelty (BERT): 0.6117763893396919
- Combined Prediction Score: 0.5857289006118053
- Mean Citation Score: 279.055944
- Max Citation Score: 649.139
- Similarity Product: 456.69445715296274

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test