PATENT CLAIM ANALYSIS

Application Number: 15864378
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-08
Patent Classification: ["530", "412000"]

Abstract:
The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.

Claim (Index 16):
The method of  claim 1 , wherein\n (a) the at least one impurity binds to the HIC media at a Kp of greater than 250, greater than 300, greater than 400, greater than 500, greater than 600, greater than 700, greater than 800, greater than 900, or greater than 1000; (b) the protein of interest and the at least one impurity have a Kp ratio less than 1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3 or 1:2; (c) the Kd for the binding of the protein of interest to the HIC media is at least about 0.2, at least about 0.3, at least about 0.4, at least about 0.5, or at least about 0.6; (d) the Kd for the binding of the at least one impurity to the HIC media is less than or equal to about 0.001, about 0.005, about 0.01, about 0.02, about 0.05, about 0.1, about 0.15 or about 0.2; (e) the Kd for the binding of the protein of interest to the HIC media is less than 50, 45, 40, 35, 30, 25, 20, 15, 10 or 5 times the Kd for the binding of the at least one impurity to the HIC media; (f) the protein of interest has a Qmax of at least about 20, at least about 30, at least about 40, at least about 50, at least about 60 or at least about 100; and/or (g) the at least one impurity has a Qmax of at least about 2, at least about 5, at least about 10, at least about 20, at least about 30 or at least about 40.

Metadata:
- Claim Count in Document: 56.0
- Percentile: 86.0
- Lexical Diversity: 1.91935
- Patent Class: 530.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14077574', '13831181', '14584492', '15545271', '14584619']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6961087959908441
- 35 USC 102 Novelty (BERT): 0.6389661229523979
- Combined Prediction Score: 0.6903945286869995
- Mean Citation Score: 392.83938
- Max Citation Score: 616.52576
- Similarity Product: 467.9208582273292

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test