PATENT CLAIM ANALYSIS

Application Number: 16069824
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2019-01
Patent Classification: ["506", "016000"]

Abstract:
The present invention relates to the field of microfluidics and in particular to analysing the gene expression of a cell in response to a ligand expressed in the same microfluidic compartment. By barcoding the transcriptome of the cell and of the expression system generating the ligand, the effect of the ligand on the cell expression can be discerned. The invention provides microfluidic compartments and methods for this purpose.

Claim (Index 11):
A method for generating a plurality of microfluidic compartments according to  claim 1 , comprising the steps of:\n (a) introducing into a microfluidic system: (i) a fluid comprising a plurality of first cells, (ii) a fluid comprising a plurality of second cells or cell-free expression systems each expressing a polypeptide ligand which is intended to specifically bind to a molecule or part thereof accessible on the surface of a first cell, and (iii) a fluid comprising sets of barcode oligonucleotides, wherein the barcode oligonucleotides of each set comprises a barcode sequence unique to the set, and a sequence capable of binding specifically to mRNA and/or cDNA, and (b) repeatedly co-compartmentalizing a first cell, a second cell or cell-free expression system, and a set of barcode oligonucleotides into microfluidic compartments, such that the size of the subset of compartments in the plurality of compartments is at least 1%.

Metadata:
- Claim Count in Document: 35.0
- Percentile: 95.0
- Lexical Diversity: 1.7561
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15847752', '15980473', '14407849', '13899397', '14586857']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5598793872542908
- 35 USC 102 Novelty (BERT): 0.5049202740317844
- Combined Prediction Score: 0.5543834759320402
- Mean Citation Score: 272.0206380000001
- Max Citation Score: 275.86694
- Similarity Product: 202.3659626139784

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test