PATENT CLAIM ANALYSIS

Application Number: 15925656
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-09
Patent Classification: ["382", "128000"]

Abstract:
Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. These have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy of polynucleotide sequencing applications.

Claim (Index 1):
A method for sequencing a plurality of polynucleotides immobilized at high density on a surface of a substrate, comprising:\n a. providing a substrate comprising a surface, wherein the surface comprises a plurality of polynucleotides immobilized on the surface at discrete locations, and wherein said surface comprises reagents for sequencing by synthesis; b. performing a plurality of cycles of single molecule sequencing by synthesis comprising, each cycle comprising:\n i. contacting said polynucleotides with a set of reversible terminator nucleotides comprising a detectable label; \n ii. imaging a field of said surface with an optical system to detect an optical signal from each nucleotide incorporated into said polynucleotides, thereby detecting a plurality of optical signals in said field for said cycle; \n c. determining a peak location from each of said plurality of optical signals from images of said field from at least two of said plurality of cycles; d. overlaying said peak locations for each optical signal and applying an optical distribution model at each cluster of optical signals to determine a relative position of each detected incorporated nucleotide on said surface with improved accuracy; e. deconvolving said optical signals in each field image from each cycle using said determined relative position and a deconvolution function; f. identifying said detectable labels incorporated into said polynucleotide for each field and each cycle from said deconvolved optical signals; and g. sequencing said plurality of polynucleotides immobilized on the surface of the substrate from said identified detectable labels across said plurality of cycles at each polynucleotide position.

Metadata:
- Claim Count in Document: 26.0
- Percentile: 90.0
- Lexical Diversity: 1.2973
- Patent Class: 382.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14366486', '12009659', '13835492', '14922038', '15207892']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.2690806792688369
- 35 USC 102 Novelty (BERT): 0.4916475780140681
- Combined Prediction Score: 0.29133736914336
- Mean Citation Score: 183.510428
- Max Citation Score: 219.60469
- Similarity Product: 167.26342310193658

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test