PATENT CLAIM ANALYSIS

Application Number: 15753318
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["435", "440000"]

Abstract:
Chromatin 3D structure modulating agents in the context of the present invention are intended to interfere or manipulate the function of loop anchor motifs, such as CTCF motifs. In certain example embodiments, the present invention may block formation of an loop anchor or chromatin domain or induce formation of a loop anchor or chromatin domain at a targeted genomic location. For instance, a loop anchor motif can be altered, such as by mutating (including inverting) a binding motif so as to remove such a motif, or by adding new binding motifs in new locations within a loop domain, so as to reduce the size of an existing loop, so as to modify the size of an existing loop, or combinations thereof. Alternatively, the chromatin 3D structure modulating agent may bind a target region and mask a loop anchor motif, thereby preventing a loop anchor or chromatin domain from forming. The chromatin 3D structure modulating agent may bind a target region and cause a loop anchor of chromatin domain to form.

Claim (Index 2):
The method of  claim 1 , wherein said interfering comprises removal of one or more existing chromatin loops or contact domains, the introduction of one or more new chromatin loops or contact domains, or the modification of one or more existing loops or contact domains,\n wherein the removal of one or more existing chromatin loops or contact domains comprises:\n i. the targeted removal or modification of one or more existing forward and/or reverse CTCF or cohesin binding motifs in or proximate to said target region; or \n ii. the targeted removal or modification of one or more, preferably all, CTCF or cohesin binding motifs located at the contact domain boundary, \n wherein the introduction of one or more new chromatin loops or contact domains comprises:\n i. the targeted introduction of one or more new forward and/or reverse CTCF or cohesin binding motifs in or proximate to said target region; or \n ii. the targeted introduction of one or more new forward and/or reverse CTCF or cohesin binding motifs in or proximate to said target region to thereby create two consecutive CTCF or cohesin binding motifs that do not loop to one another, and/or \n wherein the modification of one or more existing loops or contact domains comprises:\n i. the targeted introduction of one or more new forward and/or reverse CTCF or cohesin binding motifs; or \n ii. the targeted introduction of one or more extrusion-blocking proteins or protein-binding sites in or proximate to said target region to thereby prevent the extrusion of at least one chromatin strand through the extrusion complex whereby a smaller loop is form or a loop is blocked from forming, preferably said introduction being in a location between the forward and reverse CTCF or cohesin binding motifs at an existing loop or contact domain boundary, more preferably in a location within 1000 base pairs following an existing forward CTCF or cohesin binding motif.

Metadata:
- Claim Count in Document: 72.0
- Percentile: 88.0
- Lexical Diversity: 2.30864
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14892911', '14916338', '15179912', '14105017', '15527790']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5323410775140888
- 35 USC 102 Novelty (BERT): 0.517312549465187
- Combined Prediction Score: 0.5308382247091986
- Mean Citation Score: 257.984156
- Max Citation Score: 263.11386
- Similarity Product: 146.97621993525624

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test