PATENT CLAIM ANALYSIS

Application Number: 15995134
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-09
Patent Classification: ["435", "198000"]

Abstract:
The invention discloses a method for improving the extracellular expression level of a foreign protein by means of phospholipase fusion expression. Four proteins, PLA 2 , MBP, CBD and SUMO, are used as a fusion tag to construct a fusion gene. Compared with an original protein MOH without any fusion tag, the extracellular expression level and enzymatic activity of all the four fusion proteins are increased to some degree. Among them, the fusion protein using PLA 2  as the fusion tag has the highest expression level, which is 7.4 times higher than that of the original protein. Compared with other fusion tags, PLA 2  has a low molecular weight and the fusion protein having PLA2 as the fusion tag has the highest expression level (up to 12 g·L −1  in a 7 L fermentation tank for high-density fermentation). It is shown that the secretory expression of a foreign protein can be effectively increased by using PLA 2  as a fusion tag.

Claim (Index 11):
The method of  claim 10 , wherein the fusion tag comprises a phospholipase A2 having the amino acid sequence of SEQ ID NO: 3.

Metadata:
- Claim Count in Document: 29.0
- Percentile: 94.0
- Lexical Diversity: 2.04651
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['13395292', '14649187', '14857176', '15511822', '10592450']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5845040341441302
- 35 USC 102 Novelty (BERT): 0.5081787802493215
- Combined Prediction Score: 0.5768715087546494
- Mean Citation Score: 242.71571
- Max Citation Score: 253.06998
- Similarity Product: 176.95616234151365

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test