PATENT CLAIM ANALYSIS

Application Number: 15864378
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-08
Patent Classification: ["530", "412000"]

Abstract:
The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.

Claim (Index 25):
A method for producing a preparation comprising adalimumab and having a reduced level of at least one aggregate, said method comprising:\n (a) contacting a sample comprising adalimumab and at least one aggregate, to a HIC media, in the presence of a load buffer such that (i) a portion of the adalimumab in the sample binds to the HIC media and (ii) a substantial portion of the at least one aggregate binds to the HIC media; (b) collecting a flow through fraction comprising the adalimumab unbound to the HIC media; (c) washing the HIC media with a wash buffer that is substantially the same as the load buffer such that a substantial portion of the adalimumab bound to the HIC media is released from the media; and (d) collecting a wash fraction comprising the adalimumab released from the HIC media, wherein each of the flow through and wash fractions comprise adalimumab and have a reduced level of the at least one aggregate.

Metadata:
- Claim Count in Document: 56.0
- Percentile: 86.0
- Lexical Diversity: 1.91935
- Patent Class: 530.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14077574', '13831181', '14584492', '15545271', '14584619']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6679453460286869
- 35 USC 102 Novelty (BERT): 0.6436053594554317
- Combined Prediction Score: 0.6655113473713614
- Mean Citation Score: 392.83938
- Max Citation Score: 616.52576
- Similarity Product: 491.732255997715

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test