PATENT CLAIM ANALYSIS

Application Number: 16079740
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2019-03
Patent Classification: ["435", "006100"]

Abstract:
Provided herein is a RNA-based riboregulator that detects protein translation and uses this stimulus to regulate expression of an unrelated protein. In one embodiment, provided herein is a synthetic nucleic acid molecule configured as a translation-sensing riboregulator, where the synthetic nucleic acid molecule comprises a first nucleotide sequence encoding a polypeptide, a translation-sensing riboswitch (TSR), and a second nucleotide sequence encoding a polypeptide, wherein the translation-sensing riboswitch comprises a stop codon of the first nucleotide sequence, a fully or partially double-stranded stem-forming domain, and translation initiation elements of the second nucleotide sequence.

Claim (Index 9):
A method for detecting response of a cell to a stimulus, the method comprising\n introducing into a cell a synthetic nucleic acid molecule comprising a first nucleotide sequence encoding a first polypeptide, a translation-sensing riboswitch (TSR), and a second nucleotide sequence encoding a second polypeptide, wherein the translation-sensing riboswitch comprises a stop codon of the first nucleotide sequence, a fully or partially double-stranded stem-forming domain, and translation initiation elements of the second nucleotide sequence; detecting an expression level of each of the first and second polypeptides in the cell; exposing the cell comprising the introduced molecule to a stimulus; and detecting an expression level of each of the first and second polypeptides in the exposed cell, wherein an increase in expression of the second polypeptide following exposure relative to its expression prior to exposure indicates that translation of the first polypeptide was upregulated in the presence of the stimulus, and wherein a decrease in expression of the second polypeptide following exposure relative to its expression prior to exposure indicates that translation of the first polypeptide was downregulated in the presence of the stimulus.

Metadata:
- Claim Count in Document: 5.0
- Percentile: 96.0
- Lexical Diversity: 1.80702
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14440917', '10535128', '15364659', '10182327', '13512449']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5904624649281769
- 35 USC 102 Novelty (BERT): 0.5372526828289009
- Combined Prediction Score: 0.5851414867182494
- Mean Citation Score: 289.654948
- Max Citation Score: 377.22495
- Similarity Product: 286.9340368092209

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test