PATENT CLAIM ANALYSIS

Application Number: 16164595
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-04
Patent Classification: ["506", "016000"]

Abstract:
The disclosed embodiments relate to method, apparatus and system for high throughput single-cell DNA sequencing with droplet microfluidic. In an exemplary embodiment, a microfluidic apparatus is used to provide a rapid and cost-effective targeted genomic sequencing of thousands of cells in parallel. The targeted sequencing can be directed for residual disease detection. In one embodiment, the disclosure provides a method to detect one or more mutations in tumor cells, the method comprising: encapsulating at least one cell and a lysis reagent in a carrier fluid to form a droplet, wherein the cell originates from a tumor and the cell comprises a genomic DNA; lysing the cell to release the genomic DNA and thereby form a droplet containing the genomic DNA; introducing a cell identifier and one or more primers specific to a plurality of regions of the genomic DNA; and thermocycling the droplet to amplify the genomic DNA and to incorporate cell identifiers into the genomic DNA to produce a plurality of amplified DNA with identified loci; wherein once the cell identifier is incorporated into the amplified DNA, the identified loci are sequenced and at least one DNA mutation is identified for the tumor cells.

Claim (Index 3):
The method of  claim 1 , wherein the plurality of DNA mutations are identified substantially simultaneously for the tumor cells.

Metadata:
- Claim Count in Document: 10.0
- Percentile: 97.0
- Lexical Diversity: 2.28261
- Patent Class: 506.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14697372', '11763421', '13829971', '13656265', '12522555']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.4745082917970389
- 35 USC 102 Novelty (BERT): 0.4883992295808989
- Combined Prediction Score: 0.4758973855754249
- Mean Citation Score: 194.178922
- Max Citation Score: 206.38893
- Similarity Product: 153.35532186743615

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test