PATENT CLAIM ANALYSIS

Application Number: 16117824
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-12
Patent Classification: ["422", "502000"]

Abstract:
Disclosed herein is a multi-functional microfluidics device capable of isolation of nucleic acids, purification of nucleic acids, performance of Polymerase Chain Reactions (PCRs), in situ hybridization of nucleic acids, fluorescent signal detections and the like. The apparatus comprises an integration of a plurality of reagent chambers, a sample input, a reaction chamber, a detection chamber, and a waste chamber; wherein the plurality of reagent chambers is fluidly connected to the reaction chamber, and is configured to store a plurality of reagents; wherein the sample input is fluidly connected to the reaction chamber, and is configured to receive a sample; wherein the waste chamber is fluidly connect to the reaction chamber and is configured to receive a reaction waste from the reaction chamber.

Claim (Index 23):
A method of using an apparatus of  claim 1  for detecting a nucleic acid of interest, comprising:\n adding a sample to the detection chamber or obtaining a nucleic acid in the detection chamber; wherein the detection chamber comprises an array of molecules as probes for in situ hybridization reactions; \n adding a denaturing buffer to the detection chamber; \n subjecting the detection chamber to a denaturing temperature and dissociating a double-stranded nucleic acid in the sample into a single-stranded nucleic acid; \n subjecting the detection chamber to an annealing temperature and anneal a single-stranded nucleic acid with a complementary single stranded probe in the array of molecules; \n adding a wash buffer to the detection chamber; \n detecting a nucleic acid of interest.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 96.0
- Lexical Diversity: 2.48214
- Patent Class: 422.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13343834', '11262523', '13397416', '15906899', '13759557']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.8077715326080114
- 35 USC 102 Novelty (BERT): 0.4796928069576404
- Combined Prediction Score: 0.7749636600429742
- Mean Citation Score: 230.644414
- Max Citation Score: 235.55568
- Similarity Product: 174.1187983725357

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test