PATENT CLAIM ANALYSIS

Application Number: 15874904
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2019-07
Patent Classification: ["435", "325000"]

Abstract:
A gene knockin method and a kit for gene knockin are provided. The method comprises (a) introducing a RNA-guided endonuclease that cleaves the chromosome at the insertion site into the cell; (b) introducing a guide RNA into the cell; and (c) introducing a donor plasmid into the cell, wherein the donor plasmid comprises the donor sequence flanked with a 5′ homology arm and a 3′ homology arm, a 5′ flanking sequence upstream of the 5′ homology arm, and a 3′ flanking sequence downstream of the 3′ homology arm, wherein the 5′ homology arm is homologous to a 5′ target sequence upstream of the insertion site on the genome and the 3′ homology arm is homologous to a 3′ target sequence downstream of the insertion site on the genome, wherein the guide RNA recognizes the insertion site, the 5′ flanking sequence, and the 3′ flanking sequence, wherein the RNA-guided endonuclease cleaves the donor plasmid at the 5′ flanking sequence and the 3′ flanking sequence, thereby producing a linear nucleic acid, wherein the donor sequence is inserted in to the genome at the insertion site through homology-directed repair.

Claim (Index 9):
The method of  claim 1 , wherein the RNA-guided endonuclease is Cas9.

Metadata:
- Claim Count in Document: 47.0
- Percentile: 86.0
- Lexical Diversity: 3.21875
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15701385', '15100105', '14319380', '13889162', '14577997']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6846010798165928
- 35 USC 102 Novelty (BERT): 0.5358935019560179
- Combined Prediction Score: 0.6697303220305353
- Mean Citation Score: 370.796632
- Max Citation Score: 383.6589
- Similarity Product: 324.9913330696106

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test