PATENT CLAIM ANALYSIS

Application Number: 15893502
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["315", "149000"]

Abstract:
Laboratory optical imaging systems for fluorescence and bioluminescence use a sensitive charge-coupled device (CCD) camera to produce quantitative measurements of very low light intensity, detecting signals from specimens labeled with optical fluorophores or luminescent emitters. Commercially available systems typically provide quantitative measurements of light output, in units of radiance (photons s −1  cm −2  SR −1 ) or intensity (photons s −1  cm −2 ). We describe a quality assurance system for low-light imagers, based on an LED-illuminated thin-film transistor (TFT) liquid crystal display module. The light intensity is controlled by pulse-width modulation of the backlight, producing intensity values ranging from 1×10 6  photons s −1  cm −2  to 4×10 13  photons s −1  cm −2 . The lowest light intensity values are produced by very short backlight pulses (i.e. approximately 10 μs), repeated every 300 s. This light source provides a stable, traceable intensity standard that can be used for routine quality assurance of optical imaging systems.

Claim (Index 5):
A system for calibrating optical intensity levels and geometric scaling factors for a low-light-level imaging system, comprising:\n a. a low-intensity optical calibration phantom comprising a liquid-crystal display with pulse-width modulated backlight, the phantom suitable for imaging by a low-light level bioluminescence/luminescence imaging system; and, b. a single-board micro-controller provided with machine executable instructions configured to execute a control program that varies the temporal duty cycle of the light-emitting diode backlight for the display module; and, c. the same single-board micro-controller provided with machine executable instructions configured to execute a control program that may generate geometric control points in the intensity display, with operator-selected spacing; and, d. the same single-board micro-controller provided with machine executable instructions configured to execute a control program that may generate spatial frequency patterns in the intensity display, with operator-selected spacing; and, e. a computer provided with machine executable instructions configured to execute an analysis program that determines the light intensity from an image of the phantom acquired using the low-light level imaging system, compares the reported intensity to the true intensity of the phantom, and calculates a fitted function that relates reported intensity to true intensity; and, f. a computer provided with machine executable instructions configured to execute an analysis program that determines the light intensity from an image of the phantom acquired using the low-light level imaging system, correlates the respond intensity to the calculated variance of the light intensity signal, and calculates a fitted linear function that relates mean signal to variance; and, g. a computer provided with machine executable instructions configured to execute an analysis program that determines the location of geometric control points in an image of the phantom acquired using the low-light imaging system, compares the location of the control points in the image with the pre-determined location of the control points in the phantom, and calculates a correction that relates reported locations to true locations within the image.

Metadata:
- Claim Count in Document: 9.0
- Percentile: 88.0
- Lexical Diversity: 1.62385
- Patent Class: 315.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['10997324', '12453834', '14343612', '10068573', '10870779']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6365761209301262
- 35 USC 102 Novelty (BERT): 0.4775890654420111
- Combined Prediction Score: 0.6206774153813147
- Mean Citation Score: 154.455552
- Max Citation Score: 163.53224
- Similarity Product: 124.213872041769

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test