PATENT CLAIM ANALYSIS

Application Number: 16198902
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-03
Patent Classification: ["435", "006120"]

Abstract:
The present invention provides methods, compositions and kits for enriching and determining nucleotide sequences of a plurality of target loci from a sample comprising nucleic acids. The methods comprise one or more cycles of primer extension followed by PCR amplification of target sequences using nested target-specific primers.

Claim (Index 1):
A method of enriching a target nucleotide sequence having a locus of interest from a nucleic acid sample comprising a nucleic acid template comprising the target nucleotide sequence, the method comprising:\n (a) ligating a universal adaptor to the nucleic acid template to provide a ligated nucleic acid, wherein the universal adaptor is an oligonucleotide comprising a duplex portion at a first end and a non-duplex portion at a second end, and wherein the nucleic acid template is ligated to the universal adaptor via the first end; (b) dissociating the ligated nucleic acid into a first strand and a second strand, wherein the first strand comprises the target nucleotide sequence; (c) annealing an outside primer to the first strand of the ligated nucleic acid in the vicinity of the target nucleotide sequence; (d) extending the outside primer over the full length of the first strand of the ligated nucleic acid using a DNA polymerase to provide a nascent primer extension duplex; (e) dissociating the nascent primer extension duplex at a sufficiently high temperature into the first strand of the ligated nucleic acid and a single-stranded primer extension product; (f) repeating steps (c)-(e) for one or more primer extension cycles; (g) contacting the single-stranded primer extension products with a DNA polymerase, a universal adaptor primer and an inside primer under a condition sufficient for PCR amplification of the target nucleotide sequence, wherein the universal adaptor primer anneals to a complementary sequence of the non-duplex portion of the universal adaptor in the single-stranded primer extension products, wherein the inside primer comprises a sequence at the 3\u2032 end that specifically anneals to the target nucleotide sequence, and wherein the inside primer is nested with respect to the outside primer for the locus of interest; and (h) repeating step (g) for one or more cycles of PCR amplification to provide amplicons of the target nucleotide sequence, thereby enriching the target nucleotide sequence.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 98.0
- Lexical Diversity: 1.25
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14463498', '13793564', '15269448', '12069174', '14300048']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5622043821911126
- 35 USC 102 Novelty (BERT): 0.5316848616677363
- Combined Prediction Score: 0.5591524301387749
- Mean Citation Score: 339.0472660000001
- Max Citation Score: 356.35602
- Similarity Product: 279.5473224776602

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test