PATENT CLAIM ANALYSIS

Application Number: 15954805
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "190000"]

Abstract:
It relates to a  Mortierella alpine  ATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention,  Mortierella alpine  ATCC32222 is used as a material and undergoes gene knockout through an  Agrobacterium tumefaciens  mediated genetic manipulation technology, to obtain the  Mortierella alpine  uracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungus  Mortierella alpine  ATCC32222 and product development.

Claim (Index 3):
The method according to  claim 2 , characterized in that construction of the gene knockout plasmid comprises:\n (a) amplifying MCS DNA fragment by PCR using plasmid pBluescript II SK+ as template; (b) digesting MCS DNA fragment and plasmid pBIG2RHPH2 by EcoRI and XbaI, and ligating them together at the EcoRI and XbaI sites to form the plasmid pBIG4; (c) PCR amplifying the up- and down-stream arms of ura5 gene and ligating them together by using fusion PCR to form knockout DNA sequence; (d) digesting the KOura5 knockout DNA sequence and pBIG4 by EcoRI and KpnI, and ligating them together to form plasmid pBIG4KOura5.

Metadata:
- Claim Count in Document: 11.0
- Percentile: 91.0
- Lexical Diversity: 1.4898
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14910675', '16060417', '10454125', '12512930', '15675916']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6466942034179893
- 35 USC 102 Novelty (BERT): 0.5629913449690663
- Combined Prediction Score: 0.638323917573097
- Mean Citation Score: 337.012824
- Max Citation Score: 508.22256
- Similarity Product: 325.9588842765712

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test