PATENT CLAIM ANALYSIS

Application Number: 15771906
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-09
Patent Classification: ["435", "071100"]

Abstract:
We discovered that recombinant antibody light chains having a murine secretory leader sequence and an SYE motif at the N-terminus are truncated during post-translational processing. This disclosure provides two protein engineering solutions: to alter the SYE amino acid sequence of the Lc N-terminus to other alternatives; or to change the secretory leader peptide sequence. We have shown that both of these solutions are effective for preventing N-terminal light chain truncation.

Claim (Index 3):
A method of producing a composition comprising a homogenous population of antibody light chain (Lc) polypeptides, the method comprising:\n a. identifying a first Lc polypeptide having an amino acid sequence comprising a first nascent N-terminal sequence shown in SEQ ID NO: 5; b. expressing in a cultured cell a second Lc polypeptide, wherein the second Lc polypeptide has the same amino acid sequence as the first Lc polypeptide, except that the second Lc polypeptide comprises a second nascent N-terminal sequence selected from the group consisting of:\n SEQ ID NO: 6, \n SEQ ID NO: 7, \n SEQ ID NO: 8, \n SEQ ID NO: 9, \n SEQ ID NO: 10, \n SEQ ID NO: 11, \n SEQ ID NO: 12, \n SEQ ID NO: 13, and \n SEQ ID NO: 14; \n wherein the second Lc polypeptide is secreted into supernatant by the cultured cell, wherein the supernatant is at least 98% free of amino acid sequence variants of the second Lc polypeptide; and c. harvesting the second Lc polypeptide from the supernatant; thereby producing a composition comprising a homogenous population of antibody Lc polypeptides.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 91.0
- Lexical Diversity: 1.33929
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13934570', '12824058', '12163719', '11777777', '13800233']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5983822318079155
- 35 USC 102 Novelty (BERT): 0.5119579692001544
- Combined Prediction Score: 0.5897398055471393
- Mean Citation Score: 274.353972
- Max Citation Score: 290.66144
- Similarity Product: 213.155781283226

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test