PATENT CLAIM ANALYSIS

Application Number: 15744054
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["514", "019300"]

Abstract:
The present invention refers to new binding proteins based on di-ubiquitin modified in 12, 13, or 14 positions selected from positions R42, I44, H68, V70, R72, L73, R74, K82, L84, Q138, K139, E140, S141, and T142 of di-ubiquitin and being able to bind specifically with high affinity to selected targets. Furthermore, the invention provides a method for the generation of said binding proteins. In addition, libraries encoding for said binding proteins based on di-ubiquitin muteins modified in 12, 13, or 14 positions selected from R42, I44, H68, V70, R72, L73, R74, K82, L84, Q138, K139, E140, S141, and T142 are provided.

Claim (Index 1):
A method for generating a di-ubiquitin mutein that binds to a given target with a dissociation constant (KD) of at least 1000 nM, said method comprising:\n a. subjecting a nucleic acid molecule encoding a di-ubiquitin having an amino acid sequence as set forth in SEQ ID NO: 4 to mutagenesis in positions encoding at least 12 amino acids selected from the group consisting of R42, I44, H68, V70, R72, L73, R74, K82, L84, Q138, K139, E140, S141, and T142 di-ubiquitin to generate one or more nucleic acid molecules encoding one or more di-ubiquitin muteins; b. expressing the one or more nucleic acid molecules in a suitable expression system; and c. enriching for at least one of the one or more di-ubiquitin muteins that has a dissociation constant (KD) of at least 1000 nM for the given target via selection and/or isolation, wherein said di-ubiquitin mutein has an amino acid sequence that is between 85% and 92% identical to SEQ ID NO: 4 and has an affinity for the target that is at least 10-fold higher than does a di-ubiquitin having an amino acid sequence as set forth in SEQ ID NO: 4.

Metadata:
- Claim Count in Document: 54.0
- Percentile: 86.0
- Lexical Diversity: 2.22951
- Patent Class: 514.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14126358', '13144809', '14442376', '12293715', '13514133']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.719738465119227
- 35 USC 102 Novelty (BERT): 0.5233658241020265
- Combined Prediction Score: 0.700101201017507
- Mean Citation Score: 294.255452
- Max Citation Score: 307.40186
- Similarity Product: 220.6212902647686

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test