PATENT CLAIM ANALYSIS

Application Number: 16038389
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2018-11
Patent Classification: ["435", "006100"]

Abstract:
Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

Claim (Index 29):
A method of preparing a stained biological sample, comprising:\n (a) contacting a biological sample immobilized on a solid support with a first detection probe and a first enzyme conjugate comprising an enzyme, wherein the first detection probe binds to a first target potentially present in said biological sample and the first enzyme conjugate binds to the first detection probe, thereby forming a first binding complex comprising the first target, the first detection probe, and the first enzyme conjugate, wherein the first target comprises a polypeptide, a nucleic acid, or a tyrosine-containing moiety, and the enzyme of the first enzyme conjugate is an oxidoreductase or a peroxidase; (b) after step (a), contacting the first binding complex with a first signaling conjugate comprising a phenolic moiety and a first chromogenic moiety, such that the first signaling conjugate contacts the enzyme of the first binding complex, and the enzyme of the first binding complex catalyzes conversion of the phenolic moiety of the first signaling conjugate into a reactive species, which comprises the first chromogenic moiety, that covalently binds to at least one of: (i) a location on the first binding complex proximate to or directly on the first enzyme conjugate of the first binding complex; (ii) a location on the first binding complex proximate to or directly on the first target of the first binding complex; or (iii) a location on the biological sample proximate to the first target; wherein a first signaling complex is formed, said first signaling complex comprising the first signaling conjugate covalently bonded to said first binding complex and/or covalently bonded to the biological sample proximate to the first target; and (c) after step (b), removing the first chromogenic moiety unbound to the first signaling complex by washing the biological sample, thereby producing a stained biological sample comprising the first signaling complex on the solid support; wherein the first chromogenic moiety of the first signaling complex of the stained biological sample produces a signal when exposed to light that provides for the detection of said first target.

Metadata:
- Claim Count in Document: 56.0
- Percentile: 95.0
- Lexical Diversity: 1.96296
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13849160', '14769892', '14691826', '15246430', '15603079']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5791332171894312
- 35 USC 102 Novelty (BERT): 0.6782040789249506
- Combined Prediction Score: 0.5890403033629832
- Mean Citation Score: 419.83629200000007
- Max Citation Score: 733.6904
- Similarity Product: 647.5896580066681

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test