PATENT CLAIM ANALYSIS

Application Number: 15874673
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["435", "040500"]

Abstract:
Described herein is a bone tissue clearing method with enhanced optical access. Compositions and techniques for bone tissue clearing include continuous convective flow during the clearing process, amino alcohol to minimize tissue autofluorescence, and an imaging procedure that minimizes refractive index variations in light-sheet microscopy. These improvements allowed the Inventors to achieve whole-bone clearing with an imaging depth of up to about 1.5 mm while maintaining fluorescence and a signal-to-noise ratio (SNR) that permits detection and 3D placement of single cells. In various embodiments, the present application teaches methods and kits for clearing and optionally subsequently visualizing tissue containing bone.

Claim (Index 3):
The method of  claim 2 , wherein the fixative solution comprises 4% PFA.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 86.0
- Lexical Diversity: 1.37975
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14447607', '14932819', '13174311', '14543234', '14403050']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5479283656707338
- 35 USC 102 Novelty (BERT): 0.5271006862233297
- Combined Prediction Score: 0.5458455977259934
- Mean Citation Score: 205.693828
- Max Citation Score: 271.43472
- Similarity Product: 145.00830992786405

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test