PATENT CLAIM ANALYSIS

Application Number: 16065285
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-03
Patent Classification: ["424", "450000"]

Abstract:
The presently disclosed subject matter is directed to an RGD peptide and penetrating peptide R8 co-modified ergosterol and cisplatin active drug-loading liposome that is prepared by means of the incubation of an ergosterol and cisplatin active drug-loading liposome, RGD cyclic peptide, and penetrating peptide R8 in a water bath. The ergosterol and cisplatin active drug-loading liposome is prepared from an ergosterol liposome and a cisplatin solution serving as the raw materials. The ergosterol liposome is prepared from 8 wt % to 15 wt % ergosterol and 85 wt % to 92 wt % liposomes, and the liposomes consist of lecithin and cholesterol.

Claim (Index 9):
The RGD peptide and penetrating peptide R8 co-modified ergosterol and cisplatin active drug-loading liposome as claimed in  claim 1 , characterized in that a specific method of preparing the ergosterol and cisplatin active drug-loading liposome is as follows: (1) preparation of ergosterol liposome: lecithin, cholesterol and ergosterol are weighed, dissolved in chloroform, rotavaporized into a thin film, dried in a vacuum, hydrated with an ammonium chloride solution as a hydration solution and ultrasonically released; a probe is ultrasonically treated in an ice bath, filtered and extruded under a high pressure to generate an ergosterol liposome; (2) gradient formation of ammonium chloride: the ergosterol liposome is added to a dialysis bag with a molecular weight cut off of 8000-14000 Da; the dialysis bag is closed and dialyzed in distilled water for 2 h, and the distilled water is changed once to continue the dialysis for 2 h; (3) drug loading: cisplatin is mixed with water to make a cisplatin solution, and the dialyzed ergosterol liposome is added to the cisplatin solution for incubation, wherein the amount of ergosterol liposome added satisfies a mass ratio of ergosterol to cisplatin is 1:1 to 4:1, and the product is generated after the incubation at an incubation temperature of 40-60\u00b0 C. and an incubation time of 5-40 min

Metadata:
- Claim Count in Document: 26.0
- Percentile: 94.0
- Lexical Diversity: 2.16
- Patent Class: 424.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['15300688', '15129317', '14965140', '14966458', '14632422']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7339520360091462
- 35 USC 102 Novelty (BERT): 0.4989797455989853
- Combined Prediction Score: 0.7104548069681301
- Mean Citation Score: 215.694452
- Max Citation Score: 222.31934
- Similarity Product: 150.35065812291143

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test