PATENT CLAIM ANALYSIS

Application Number: 15991370
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2019-08
Patent Classification: ["435", "100000"]

Abstract:
The present invention discloses a maltooligosyl trehalose trehalohydrolase (MTHase) mutant and application thereof, belonging to the technical fields of gene engineering and enzyme engineering. The present invention provides a series of MTHase mutants to prepare trehalose, having a better effect. Further, the MTHase mutant is expressed in  Escherichia coli  BL21 (DE3) and the enzyme is optimized by fermentation, which can significantly increase the yield of enzymes.

Claim (Index 2):
The mutant of  claim 1 , comprising comprises an amino acid sequence with mutations selected from the group consisting of:\n mutation of methionine (Met) at position 140 to leucine (Leu), and the mutant is named M140L; mutation of leucine (Leu) at position 202 to proline (Pro), and the mutant is named L202P; mutation of leucine (Leu) at position 218 to aspartic acid (Asp), and the mutant is named L218D; mutation of tyrosine (Tyr) at position 323 to glycine (Gly), and the mutant is named Y323G; mutation of phenylalanine (Phe) at position 338 to serine (Ser), and the mutant is named F338S; mutation of isoleucine (Ile) at position 404 to threonine (Thr), and the mutant is named I404T; mutation of leucine (Leu) at position 202 to proline (Pro), and mutation of tyrosine (Tyr) at position 323 to glycine (Gly), and the mutant is named L202P/Y323G; mutation of leucine (Leu) at position 202 to proline (Pro), mutation of leucine (Leu) at position 218 to aspartic acid (Asp), and mutation of tyrosine (Tyr) at position 323 to glycine (Gly), and the mutant is named L202P/L218D/Y323G; mutation of methionine (Met) at position 140 to leucine (Leu), mutation of leucine (Leu) at position 202 to proline (Pro), mutation of leucine (Leu) at position 218 to aspartic acid (Asp), and mutation of tyrosine (Tyr) at position 323 to glycine (Gly), and the mutant is named M140L/L202P/L218D/Y323G; mutation of methionine (Met) at position 140 to leucine (Leu), mutation of leucine (Leu) at position 202 to proline (Pro), mutation of leucine (Leu) at position 218 to aspartic acid (Asp), mutation of tyrosine (Tyr) at position 323 to glycine (Gly), and mutation of phenylalanine (Phe) at position 338 to serine (Ser), and the mutant is named M140L/L202P/L218D/Y323G/F338S; and mutation of methionine (Met) at position 140 to leucine (Leu), mutation of leucine (Leu) at position 202 to proline (Pro), mutation of leucine (Leu) at position 218 to aspartic acid (Asp), mutation of tyrosine (Tyr) at position 323 to glycine (Gly), mutation of phenylalanine (Phe) at position 338 to serine (Ser), and mutation of isoleucine (Ile) at position 404 to threonine (Thr), and the mutant is named M140L/L202P/L218D/Y323G/F338S/I404T.

Metadata:
- Claim Count in Document: 3.0
- Percentile: 93.0
- Lexical Diversity: 1.54
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['13210646', '11418804', '12189232', '15820487', '11611058']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5695162877241393
- 35 USC 102 Novelty (BERT): 0.5043921620725799
- Combined Prediction Score: 0.5630038751589834
- Mean Citation Score: 263.514026
- Max Citation Score: 267.1345
- Similarity Product: 202.82363256895545

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test