PATENT CLAIM ANALYSIS

Application Number: 16157930
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-02
Patent Classification: ["435", "347000"]

Abstract:
The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

Claim (Index 8):
A method of determining the hepatotoxicity of an interaction between a first test compound and a second test compound, the method comprising:\n obtaining a co-culture of a population of hepatocytes derived from induced pluripotent stem cells and a population of 3T3-J3 fibroblasts in vitro, with a layer of material comprising a gelatinous protein mixture secreted by Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells disposed over the co-culture; contacting the co-culture with the first and second test compounds; maintaining the co-culture for a time and under conditions sufficient to allow an effect of an interaction the first and second test compounds on the stem cell-derived hepatocytes; measuring at least one of indicator of hepatic function in the hepatocytes to obtain a test measurement, or applying hepatocyte imaging technology (HIAT) to the hepatocytes to obtain a test image; and comparing the test measurement to a control measurement from the hepatocytes before contact with the first and second test compounds, or the test image to a control image before contact with the first and second test compounds, wherein a difference between the test and control is indicative of hepatotoxicity of the interaction between the first test compound and the second test compound, wherein the cell populations are disposed in a micropattern on a culture substrate and the micropattern comprises a predetermined two-dimensional pattern of multiple microdots, the micropattern defined by a microdot diameter and a center-to-center spacing between each of any two neighboring microdots, wherein each microdot has a diameter of about 500 \u03bcm and the center-to-center spacing between each of any two neighboring microdots is about 1200 \u03bcm, and the microdots comprise the human hepatocytes derived from induced human pluripotent stem cells and the space between the microdots comprises the 3T3-J3 fibroblast population, and wherein the population of hepatocytes in co-culture exhibits a higher level of cytochrome P450 3A4 (CYP3A4) enzyme activity by at least day 22 of culture as compared to a population of human hepatocytes derived from induced pluripotent stem cells and not co-cultured with a population of 3T3-J3 fibroblasts.

Metadata:
- Claim Count in Document: 4.0
- Percentile: 97.0
- Lexical Diversity: 1.54902
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14596787', '13267866', '15006754', '14772284', '13772802']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5675249513754416
- 35 USC 102 Novelty (BERT): 0.7161257293487864
- Combined Prediction Score: 0.5823850291727761
- Mean Citation Score: 434.565556
- Max Citation Score: 874.7666
- Similarity Product: 741.5376653273582

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test