PATENT CLAIM ANALYSIS

Application Number: 15910218
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-07
Patent Classification: ["424", "093700"]

Abstract:
The present invention provides a method for isolating human neural stem cells from amniotic fluid of a patient whose fetus has been diagnosed to have a neural tube defect. Use of the isolated human neural stem cells in the treatment of neurological disorders is also provided.

Claim (Index 10):
A method for testing cytotoxicity of a drug candidate comprising: contacting an isolated human neural stem cells with the drug candidate; and determining one or more cell conditions of the cells, if the determined one or more cell conditions are poorer than the same condition(s) of the cells without contacting the drug candidate, it represents that the drug candidate may have cytotoxicity,\n wherein the isolated human neural stem cells are obtained from a method consisting of: (a) collecting the cells from amniotic fluid obtained from a pregnant human subject whose fetus has been diagnosed to have a neural tube defect; (b) incubating the cells with a culture medium; (c) detecting expression levels of Nestin, Sox2, Musashi-1, and ATP-binding cassette G2 (ABCG2) markers and activity of Stage-Specific Embryonic Antigen-3 (SSEA-3), Stage-Specific Embryonic Antigen-4 (SSEA-4), Tumor Rejection Antigen-1-60 (TRA-1-60), TRA-1-81 and telomerase in the cells; and (d) isolating from the culture medium human neural stem cells that express Nestin, Sox2, Musashi-1 and ABCG2 markers and exhibit telomerase activity but do not express SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81 from the culture medium.

Metadata:
- Claim Count in Document: 21.0
- Percentile: 90.0
- Lexical Diversity: 1.2973
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14660554', '14396180', '12182050', '15297972', '13504988']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.70063346083257
- 35 USC 102 Novelty (BERT): 0.6774229289029408
- Combined Prediction Score: 0.6983124076396071
- Mean Citation Score: 424.68344
- Max Citation Score: 824.89417
- Similarity Product: 630.1967734765685

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test