PATENT CLAIM ANALYSIS

Application Number: 15746621
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2019-03
Patent Classification: ["435", "254230"]

Abstract:
The invention discloses a high-secretion heat-resistant yeast genetically engineered strain and application thereof, belonging to the field of biotechnology. Mutagenesis and domestication are performed to obtain the yeast genetically engineered strain capable of expressing a lipase gene at a high secretion level at high temperature. The strain is collected by China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC NO: M 2016278. The sequencing analysis shows that the lipase gene and promoter sequence thereof are not mutated, which indicates that the high-secretion expression of the lipase gene by the mutant strain is caused by mutation of other gene sequences in the genome. By knocking out the lipase gene in homologous double-crossover way, the constructed knockout strain can be used as an expression host of other exogenous proteins, can be used for enhancing the expression level of other exogenous genes, and can be subjected to fermentation under high-temperature fermentation conditions.

Claim (Index 2):
A recombinant  Pichia pastoris , which is obtained by modification on the basis of the  Pichia pastoris  CCTCC NO: M 2016278.

Metadata:
- Claim Count in Document: 12.0
- Percentile: 91.0
- Lexical Diversity: 1.7766
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['12836864', '15876158', '12866453', '13515577', '14354628']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6268273098385215
- 35 USC 102 Novelty (BERT): 0.5032192536320508
- Combined Prediction Score: 0.6144665042178745
- Mean Citation Score: 228.60634
- Max Citation Score: 242.3138
- Similarity Product: 158.35535183575152

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test