PATENT CLAIM ANALYSIS

Application Number: 15974429
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2018-12
Patent Classification: ["435", "053000"]

Abstract:
The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.

Claim (Index 24):
A method of producing a mogrol precursor, a mogroside precursor, and/or a mogroside compound, comprising whole cell bioconversion of a plant-derived or a synthetic mogrol precursor or a mogroside precursor in a cell culture medium of a recombinant host cell using:\n (a) a polypeptide capable of catalyzing conversion of oxido-squalene to produce cucurbitadienol;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NOs:1 or 43; \n (b) a polypeptide capable of catalyzing conversion of dioxido-squalene to produce 24,25 epoxy cucurbitadienol;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NOs:1 or 43; \n (c) a polypeptide capable of catalyzing hydroxylation of cucurbitadienol to produce 11-hydroxy-cucurbitadienol;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in any one of SEQ ID NOs:3-20, or 41 or the amino acid sequence set forth in SEQ ID NO:44; \n (d) a polypeptide capable of catalyzing conversion of 11-hydroxy-cucurbitadienol to produce mogrol;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in SEQ ID NOs:38 or 40; \n (e) a polypeptide capable of catalyzing epoxidation of 11-hydroxy-cucurbitadienol to produce 11-hydroxy-24,25 epoxy cucurbitadienol;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in any one of SEQ ID NOs:3-20, or 41 or the amino acid sequence set forth in SEQ ID NO:44; \n (f) a polypeptide capable of catalyzing conversion of 11-hydroxy-24,25 epoxy cucurbitadienol to produce mogrol;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in SEQ ID NOs:38 or 40; \n (g) a polypeptide capable of catalyzing epoxidation of cucurbitadienol to produce 24,25 epoxy cucurbitadienol;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in any one of SEQ ID NOs:3-20, or 41 or the amino acid sequence set forth in SEQ ID NO:44; \n (h) a polypeptide capable of catalyzing hydroxylation of 24,25 epoxy cucurbitadienol to produce 11-hydroxy-24,25 epoxy cucurbitadienol;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by a nucleotide sequence set forth in any one of SEQ ID NOs:3-20, or 41 or the amino acid sequence set forth in SEQ ID NO:44; and/or \n (i) a polypeptide capable of glycosylation at C3\u2032-OH, C24\u2032-OH, C3\u2032-OH and C24\u2032-OH of the mogrol, at C3\u2032-OH or C24\u2032-OH of the glycosylated mogroside compound, or both C3\u2032-OH, C24\u2032-OH, C3\u2032-OH and C24\u2032-OH of the mogrol and C3\u2032-OH or C24\u2032-OH of the glycosylated mogroside compound or beta-1,6-glycosylation of the C2\u2032 position of the 24-O-glucose, and/or beta-1,2-glycosylation of the C6\u2032 position of the 3-O-glucose and/or the 24-O-glucose of the glycosylated mogroside compound;\n wherein the polypeptide comprises a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in any one of SEQ ID NOs: SEQ ID NOs:21-25, 48, 50, or 53 and/or a polypeptide having at least 90% sequence identity to the amino acid sequence encoded by the nucleotide sequence set forth in any one of SEQ ID NOs:26-36; \n wherein at least one of the polypeptides is a recombinant polypeptide expressed in the recombinant host cell; \n wherein the recombinant host cell is a microorganism that is a plant cell, a mammalian cell, an insect cell, a fungal cell, an algal cell, or a bacterial cell; \n and producing the mogrol precursor, the mogroside precursor, and/or the mogroside compound thereby.

Metadata:
- Claim Count in Document: 65.0
- Percentile: 93.0
- Lexical Diversity: 1.54545
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15900557', '14504109', '14442694', '15511565', '15519326']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7106639145171985
- 35 USC 102 Novelty (BERT): 0.7623842104390155
- Combined Prediction Score: 0.7158359441093802
- Mean Citation Score: 758.4892920000003
- Max Citation Score: 915.48206
- Similarity Product: 884.7332376863122

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test