PATENT CLAIM ANALYSIS

Application Number: 16042284
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2019-08
Patent Classification: ["530", "333000"]

Abstract:
The embodiments herein disclose a method for synthesizing antagonistic peptide VEGF and bFGF. The method comprises synthesizing the antagonistic peptide for VEGF and bFGF and analyzing the purity of peptides. The quality of antagonistic peptide for VEGF and bFGF is analyzed by HPLC chromatogram and Mass spectrometry analysis. The biochemical activity of the antagonistic peptide for VEGF and bFGF is analyzed by competitive binding assay, cell proliferation assay, Matrigel assay for anti-angiogenic activity analysis, histopathological staining and Western blot analysis. The competitive binding assay of antagonistic peptide for VEGF and bFGF illustrate that peptides binds with cell receptors at a concentration of 2000 ng/ml. The cell proliferation assay illustrates that cell growth is arrested when antagonistic peptide for VEGF and bFGF are at a concentration of 2000 ng/ml. The anti-angiogenic activity analysis illustrates that angiogenesis is arrested when the concentration of antagonistic peptide for VEGF and bFGF is 2000 ng/ml.

Claim (Index 5):
The method according to  claim 1 , wherein the Seq. ID No.4 peptide comprises amino acid residues derived from VEGF-A, and wherein Seq. ID No.4 comprises of amino acid residues 34-51 in VEGF-A, and where the Seq. ID No.4 comprises a biochemical structure represented by  2 HN-Asp 34 -Ile 35 -Phe 36 -Gln 37 -Glu 38 -Tyr 39 -Pro 40 -Asp 41 -Glu 42 -Ile 43 -Glu 44 -Tyr 45 -Ile 46 -Phe 47 -Lys 48 -Pro 49 -Ser 50 -Cys 51 -COOH, and wherein a cysteine residue is added optionally to the N-terminus of Seq.ID No.4 for chain cyclization, and wherein the Seq. ID No.4 peptide binds and blocks vascular endothelial growth factor receptor 2 (VEGFR-2).

Metadata:
- Claim Count in Document: 15.0
- Percentile: 95.0
- Lexical Diversity: 2.75862
- Patent Class: 530.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15222237', '10263162', '10046922', '13713594', '13662024']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.643835552643319
- 35 USC 102 Novelty (BERT): 0.5462906140746171
- Combined Prediction Score: 0.6340810587864488
- Mean Citation Score: 381.339348
- Max Citation Score: 398.11826
- Similarity Product: 252.18515295695187

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test