PATENT CLAIM ANALYSIS

Application Number: 15744198
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["435", "007100"]

Abstract:
Platform technology involving aqueous microdroplet reaction vessels created, arrayed, and characterized by imaging microscopy in a microfluidic device are applied to a wide variety of bioassays involving the detection and phenotypic characterization of single cells. The bioassays include the rapid and automated detection of microbial pathogens and their antibiotic sensitivity from patient samples as well as the characterization of immune responses using a patient's own cells, including the killing of tumor cells.

Claim (Index 1):
A method of detecting bacterial cells, the method comprising the steps of:\n (a) providing a microfluidic device capable of forming aqueous microdroplets in oil, the device comprising a translucent microdroplet array chamber, and providing a fluorescence imaging microscope; (b) preparing a plurality of aqueous microdroplets in oil using the microfluidic device, each microdroplet comprising a sample containing or suspected of containing one or more bacterial cells, one or more microbeads conjugated with a capture antibody capable of specifically binding a selected type of bacterial cell at a first epitope, and a fluorescently labeled detection antibody capable of specifically binding the selected type of bacterial cell at a second epitope; (c) directing the plurality of aqueous microdroplets into the microdroplet array chamber; (d) obtaining a fluorescence image of the microdroplet array chamber using the fluorescence imaging microscope; and (e) measuring fluorescence emission from the labeled detection antibody in images of the microbeads, whereby the presence of the selected type of bacterial cell in the sample is detected when fluorescence emission from the labeled detection antibody overlaps with an image of one or more of said microbeads.

Metadata:
- Claim Count in Document: 38.0
- Percentile: 86.0
- Lexical Diversity: 1.41818
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15317393', '14420646', '14982458', '11667277', '13970724']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.60986382079771
- 35 USC 102 Novelty (BERT): 0.5121639873831976
- Combined Prediction Score: 0.6000938374562587
- Mean Citation Score: 250.354318
- Max Citation Score: 286.97946
- Similarity Product: 184.6289212812496

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test