PATENT CLAIM ANALYSIS

Application Number: 16012635
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["506", "016000"]

Abstract:
Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.

Claim (Index 1):
A method for sample identification, comprising:\n (a) contacting each of a plurality of samples comprising one or more cells with a first oligonucleotide-conjugated antibody to form a plurality of barcoded samples, respectively, each comprising one or more cells associated with a first oligonucleotide-conjugated antibody,\n wherein each of the one or more cells comprises a protein target for sample indexing, \n wherein the first antibody is capable of specifically binding to the protein target for sample indexing, and is conjugated with a sample indexing oligonucleotide comprising a poly(A) tail and a sample indexing sequence, and \n wherein the sample indexing sequences of the first oligonucleotide-conjugated antibodies associated with cells of different barcoded samples comprise different sequences; \n (b) pooling the plurality of barcoded samples to form a combined barcoded sample comprising a plurality of cells associated with the first oligonucleotide-conjugated antibodies; (c) partitioning the plurality of cells from the combined barcoded sample to a plurality of partitions, wherein a partition of the plurality of partitions comprises a single cell from the plurality of cells associated with the first oligonucleotide-conjugated antibodies; (d) in the partition comprising the single cell, contacting a barcoding particle with the sample indexing oligonucleotide, wherein the barcoding particle comprises a plurality of oligonucleotide probes each comprising a poly(T) region and a barcode sequence common to the plurality of oligonucleotide probes; (e) extending the oligonucleotide probes hybridized to the sample indexing oligonucleotide via the hybridization between the poly(A) tails of the sample indexing oligonucleotides and the poly(T) region of the oligonucleotide probes to produce a first plurality of labeled nucleic acids, wherein each of the first plurality of labeled nucleic acid comprises a sample indexing sequence, or a complementary sequence thereof, and the barcode sequence; and (f) obtaining sequencing information of the first plurality of labeled nucleic acids or a portion thereof to determine sample origins of one or more of the plurality of cells in the combined barcoded sample.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 94.0
- Lexical Diversity: 1.81818
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15937713', '15993468', '15055445', '15667125', '15847752']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.583869226473468
- 35 USC 102 Novelty (BERT): 0.6483812834611012
- Combined Prediction Score: 0.5903204321722313
- Mean Citation Score: 477.22066800000016
- Max Citation Score: 676.4635599999998
- Similarity Product: 571.401940667641

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test