PATENT CLAIM ANALYSIS

Application Number: 16070661
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2019-02
Patent Classification: ["435", "198000"]

Abstract:
Disclosed are various enzymatic processes for the enrichments of oils with omega-3 fatty acids, and specific lipase preparations for use with these processes.

Claim (Index 20):
A process for enzymatic preparation of n-3 fatty acid short-chain alkyl esters, specifically n-3 fatty acid ethyl esters, comprising:\n (a) providing an oil comprising n-3 polyunsaturated fatty acids, specifically from about 5% to about 60% w/w, said n-3 fatty acids being present as fatty acyl groups bonded to the glycerol backbone of glycerides and optionally of polar lipids in said oil and/or as free fatty acids, said oil also comprising free or esterified saturated fatty acids, mono-, di- and tri-unsaturated fatty acids; (b) adding to said oil, water or alkaline aqueous buffer solution at an amount of 0.2-10% w/w of said oil, to give a mixture with pH of from about 5 to about 9; (c) adding to the mixture obtained in step (b) a  Pseudomonas  sp. lipase (PS lipase) preparation in which said PS lipase immobilized on a polymeric resin support at a high load, specifically higher than 10,000 units of said PS lipase per Ig of polymer support, wherein said resin comprises alternating hydrophobic domains and mild hydrophilic domains, specifically hydrophobic domains comprised of hydrophobic aliphatic or aromatic monomers and mild hydrophilic domains comprised of acrylic and/or methacrylic esters, wherein said lipase PS exhibits high selectivity towards saturated, mono-, di- and tri-unsaturated fatty acids in their free form or when bound to glycerol or to polar lipids, and is capable of simultaneously or consecutively transesterifying/esterifying said fatty acids; (d) providing a short-chain alkyl alcohol, specifically ethanol, and adding, specifically step-wise adding, said alcohol to the mixture of said oil, said water or alkaline aqueous buffer solution and said enzyme preparation obtained in step (c), to form a reaction system comprising said oil, said lipase preparation, said water or aqueous buffer solution and said alcohol, wherein said oil reacts with said alcohol, and allowing said reaction to reach a conversion of about 70% of said oil to fatty acid alkyl esters; (e) stopping the reaction by removing the said first lipase preparation and collecting the organic upper phase/fraction of the reaction mixture containing fatty acids alkyl esters (FAEEs), mono-, di- and the residual tri-glycerides, in addition to free fatty acids byproducts and excess of alcohol; (f) reacting the said organic phase obtained in step (e) with a short-chain alkyl alcohol, specifically ethanol, in the presence of  Thermomyces lanuginosa  lipase (TL) immobilized on a polymeric resin support, wherein said resin comprises alternating hydrophobic domains and mild hydrophilic domains, specifically hydrophobic domains comprised of hydrophobic aliphatic or aromatic monomers and mild hydrophilic domains comprised of acrylic and/or methacrylic esters, and allowing the reaction to continue until at least 97% w/w, specifically 98% w/w, 99% w/w and up to 100% of fatty acyl groups in said oil are converted to their lower alkyl esters, specifically ethyl esters; (g) stopping the reaction by removing the said lipase preparation from the reaction mixture followed with evaporation, specifically flash evaporation of the reaction medium to remove excess of alcohol and water; and (h) isolating said n-3 polyunsaturated fatty acid alkyl esters concentrate from the reaction mixture by fractional distillation.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 95.0
- Lexical Diversity: 1.13636
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12744761', '11946121', '15984796', '13597304', '14143258']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6607207054540727
- 35 USC 102 Novelty (BERT): 0.5467381917583264
- Combined Prediction Score: 0.6493224540844982
- Mean Citation Score: 380.249906
- Max Citation Score: 431.8138
- Similarity Product: 315.1198428981543

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test