PATENT CLAIM ANALYSIS

Application Number: 15754061
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-12
Patent Classification: ["514", "04400A"]

Abstract:
The disclosure includes methods of determining the activity of an inhibitor of a target microRNA (miRNA) or of a mimic of a target microRNA. Determination of polysome occupancy in treated and control samples followed by comparing the occupancies can be used to determine a displacement value for the inhibitor or mimic of the target miRNA. The displacement value reflects the extent of a change in the levels of the target miRNA (which may include the mimic if applicable) in polysomal and non-polysomal compartments of a sample or a shift of the target miRNA between polysomal and non-polysomal compartments of a sample and can indicate the activity of the inhibitor toward the target miRNA or of the mimic toward a target RNA.

Claim (Index 31):
The method of  claim 1 , wherein:\n a) determining the polysome occupancy of the target microRNA in the treated sample comprises\n i) measuring the amount of the target microRNA in the treated sample by quantitative PCR to generate a target microRNA Ct value for the treated sample; \n ii) measuring the amount of a reference RNA in the treated sample by quantitative PCR to generate a reference RNA Ct value for the treated sample; and \n iii) subtracting the reference RNA Ct value for the treated sample from the target microRNA Ct value for the treated sample, wherein the resulting value is the polysome occupancy for the treated sample; \n b) determining the polysome occupancy of the target microRNA in the control sample comprises\n i) measuring the amount of the target microRNA in the control sample by quantitative PCR to generate a target microRNA Ct value for the control sample; \n ii) measuring the amount of a reference microRNA in the control sample by quantitative PCR to generate a reference RNA Ct value for the control sample; and \n iii) subtracting the reference RNA Ct value for the control sample from the target microRNA Ct value for the control sample, wherein the resulting value is the polysome occupancy for the control sample.

Metadata:
- Claim Count in Document: 46.0
- Percentile: 88.0
- Lexical Diversity: 2.24561
- Patent Class: 514.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['13964705', '14866186', '11100897', '13415685', '14061350']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7373015070898495
- 35 USC 102 Novelty (BERT): 0.5083339483700499
- Combined Prediction Score: 0.7144047512178696
- Mean Citation Score: 251.677988
- Max Citation Score: 263.2443
- Similarity Product: 184.77718506916165

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test