PATENT CLAIM ANALYSIS

Application Number: 15945549
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "118000"]

Abstract:
The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

Claim (Index 1):
A process for preparing a compound of structural Formula (I): having the indicated stereochemical configuration at the stereogenic center marked with an *; in an enantiomeric excess of at least 70% over the opposite enantiomer, wherein\n Z is OR 2  or NR 2 R 3 ; \n R 1  is C 1-8  alkyl, aryl, heteroaryl, aryl-C 1-2  alkyl, heteroaryl-C 1-2  alkyl, or a 5- to 6-membered heterocyclic ring system optionally containing an additional heteroatom selected from O, S, and N, the heterocyclic ring being unsubstituted or substituted with one to three substituents independently selected from oxo, hydroxy, halogen, C 1-4  alkoxy, and C 1-4  alkyl, wherein alkyl and alkoxy are unsubstituted or substituted with one to five fluorines; \n R 2  and R 3  are each independently hydrogen, C 1-8  alkyl, aryl, or aryl-C1-2 alkyl; or \n R 2  and R 3  together with the nitrogen atom to which they are attached form a 4- to 7-membered heterocyclic ring system optionally containing an additional heteroatom selected from O, S, and N, the heterocyclic ring being unsubstituted or substituted with one to three substituents independently selected from oxo, hydroxy, halogen, C 1-4  alkoxy, and C 1-4  alkyl, wherein alkyl and alkoxy are unsubstituted or substituted with one to five fluorines; and the heterocyclic ring system being optionally fused with a 5- to 6-membered saturated or aromatic carbocyclic ring system or a 5- to 6-membered saturated or aromatic heterocyclic ring system containing one to two heteroatoms selected from O, S, and N, the fused ring system being unsubstituted or substituted with one to two substituents selected from hydroxy, amino, fluorine, C 1-4  alkyl, C 1-4  alkoxy, and trifluoromethyl; the process comprising the step of contacting a compound of structural Formula (II): \n with an engineered polypeptide having transaminase activity comprising an amino acid sequence having at least 80% sequence identity to reference sequence of SEQ ID NO:2 and an amino acid residue difference in position 215 of SEQ ID NO:2, in the presence of an amino group donor in a suitable organic solvent under suitable reaction conditions.

Metadata:
- Claim Count in Document: 101.0
- Percentile: 91.0
- Lexical Diversity: 1.66667
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15443792', '14768408', '14342713', '15449677', '14799180']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6764934903459063
- 35 USC 102 Novelty (BERT): 0.690920156942878
- Combined Prediction Score: 0.6779361570056035
- Mean Citation Score: 534.0153799999998
- Max Citation Score: 735.4903
- Similarity Product: 709.5318203707753

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test