PATENT CLAIM ANALYSIS

Application Number: 16198902
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-03
Patent Classification: ["435", "006120"]

Abstract:
The present invention provides methods, compositions and kits for enriching and determining nucleotide sequences of a plurality of target loci from a sample comprising nucleic acids. The methods comprise one or more cycles of primer extension followed by PCR amplification of target sequences using nested target-specific primers.

Claim (Index 6):
The method according to  claim 1 , characterized in that:\n (1) the amplicons of the target nucleotide sequence are used for next-generation sequencing (NGS), and the nucleic acid template is fragmented to a size suitable for the next-generation sequencing; (2) the method further comprising end repairing and A-tailing of the nucleic acid template prior to step (a); (3) the non-duplex portion of the universal adaptor comprises a 3\u2032 end having a blocking moiety, optionally, the blocking moiety is an inverted nucleotide, for example, the blocking moiety is a stretch of flapping nucleotides having one or more phosphorothioate modifications; (4) the non-duplex portion of the universal adaptor comprises a molecular barcode comprising degenerately designed nucleobases, the duplex portion of the universal adaptor comprises a sample barcode, optionally, the sample barcode is located at the first end of the universal adaptor, for example, the sample barcode consists of about 4-13 nucleotides; (5) the first end of the universal adaptor comprises constant nucleobases of a sufficiently short length to prevent promiscuous priming during steps (b)-(f) by carryover universal adaptor; (6) the sufficiently high temperature is at least about 90\u00b0 C.; (7) the ligated nucleic acid is subjected to a cleanup procedure prior to step (b); (8) the primer extension products are subjected to a cleanup procedure prior to step (g); (9) step (g) is repeated for about 2-100 cycles; and/or (10) the amplicons of the target nucleotide sequence are used for next-generation sequencing (NGS), and the universal adaptor or the 5\u2032 end of the universal adaptor primer comprises a sequence identical or complementary to the sequence of a first sequencing primer for the NGS, optionally, step (g) comprises contacting the single-stranded primer extension products with a DNA polymerase, a universal adaptor primer, an inside primer, and a sequencing adaptor primer under a condition sufficient for PCR amplification of the target nucleotide sequence, wherein the sequencing adaptor primer comprises at the 3\u2032 end a sequence identical to a sequence of the inside primer, and at the 5\u2032 end a sequence identical or complementary to the sequence of a second sequencing primer for the NGS.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 98.0
- Lexical Diversity: 1.25
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14463498', '13793564', '15269448', '12069174', '14300048']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5622701326408769
- 35 USC 102 Novelty (BERT): 0.53182105485075
- Combined Prediction Score: 0.5592252248618642
- Mean Citation Score: 339.0472660000001
- Max Citation Score: 356.35602
- Similarity Product: 275.5157530723357

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test