PATENT CLAIM ANALYSIS

Application Number: 15926992
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-09
Patent Classification: ["422", "500000"]

Abstract:
The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

Claim (Index 16):
A method of forming a plurality of plugs and conducting a reaction in at least one thereof, the method comprising the steps of:\n providing a microfluidic device comprising a substrate having at least a first channel and a second channel intersection each other at a junction; providing an aqueous fluid containing target DNA or RNA molecules and at least one other molecule in the fluid that can react with the target DNA or RNA molecules under conditions in which the target DNA or RNA molecules and the other molecules in the aqueous fluid do not react with each other; introducing to the first channel of the microfluidic device, and continuously flowing, the aqueous fluid containing the target DNA or RNA molecules and at least one other molecule in the fluid that can react with the target DNA or RNA molecules; introducing to the second channel of the microfluidic device, and continuously flowing, a carrier fluid comprising an oil; controlling flow rates of the aqueous fluid and the carrier fluid to partition the continuously flowing aqueous fluid with the continuously flowing carrier fluid to form a plurality of plugs of the aqueous fluid, each of the plurality of plugs being substantially surrounded by the carrier fluid and each having a substantially uniform size of about 200 \u03bcm or less, wherein the target DNA or RNA in the plurality of plugs represents a Poisson distribution, and at least one of the plurality of plugs comprises a single target DNA or RNA molecule and at least one of the other molecules that can react with the target DNA or RNA molecule; and providing conditions suitable for a polymerase-chain reaction in the at least one of the plurality of plugs such that the target DNA or RNA is amplified.

Metadata:
- Claim Count in Document: 20.0
- Percentile: 90.0
- Lexical Diversity: 1.27273
- Patent Class: 422.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13563347', '13024145', '13024171', '13024155', '14517602']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.8501460607140918
- 35 USC 102 Novelty (BERT): 0.552759898302228
- Combined Prediction Score: 0.8204074444729055
- Mean Citation Score: 437.838312
- Max Citation Score: 467.76465
- Similarity Product: 383.3959042899013

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test