PATENT CLAIM ANALYSIS

Application Number: 16058777
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2019-01
Patent Classification: ["250", "282000"]

Abstract:
The present invention is based on the finding that enrichment of D3-creatinine in a urine sample following oral administration of a single defined dose of D3-creatine can be used to calculate total-body creatine pool size and total body skeletal muscle mass in a subject. The invention further encompasses methods for detecting creatinine and D3-creatinine in a single sample. The methods of the invention find use, inter alia, in diagnosing disorders related to skeletal muscle mass, and in screening potential therapeutic agents to determine their effects on muscle mass.

Claim (Index 1):
A method of determining total body skeletal muscle mass in a subject, comprising:\n (a) orally administering D 3 -creatine to a subject, wherein the D 3 -creatine dilutes in the total body skeletal muscle pool of creatine and reaches isotopic steady-state in the total body skeletal muscle pool of creatine; (b) obtaining a biological sample comprising creatinine and D 3 -creatinine from the subject, wherein the biological sample is selected from the group consisting of a blood sample, a serum sample, a plasma sample, and a tissue sample; (c) detecting creatinine and D 3 -creatinine in the biological sample by a method selected from the group consisting of HPLC/MS, HPLC/MS/MS, LCMS, LC/MS/MS, and isotope ratio mass spectrometry (IRMS); (d) measuring enrichment ratio of D 3 -creatinine in the biological sample at a time t based on the creatinine and D 3 -creatinine detected in (c); (e) measuring a total amount of urinary D 3 -creatinine from administration of the D 3 -creatine to the time t; (f) determining total-body creatine pool size in the subject by the formula [ Amount \ue89e \ue89e of \ue89e \ue89e D 3 \ue89e - \ue89e creatine \ue89e \ue89e dosed \ue89e \ue89e ( g ) - Total \ue89e \ue89e amount \ue89e \ue89e of \ue89e \ue89e urinary \ue89e \ue89e D 3 \ue89e - \ue89e creatine \ue89e \ue89e ( o - t ) \ue89e ( g ) ] enrichment \ue89e \ue89e ratio \ue89e \ue89e ( t ) = total \ue89e - \ue89e body \ue89e \ue89e creatine \ue89e \ue89e pool \ue89e \ue89e size ; and\n (g) determining total body skeletal muscle mass in the subject based on the formula \n Total \ue89e \ue89e body \ue89e \ue89e skeletal \ue89e \ue89e muscle \ue89e \ue89e mass = ( the \ue89e \ue89e total \ue89e - \ue89e body \ue89e \ue89e creatine \ue89e \ue89e pool \ue89e \ue89e size creatine \ue89e \ue89e concentration \ue89e \ue89e in \ue89e \ue89e skeletal \ue89e \ue89e muscle ) .

Metadata:
- Claim Count in Document: 30.0
- Percentile: 96.0
- Lexical Diversity: 1.54098
- Patent Class: 250.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15098217', '14401135', '14210415', '13332773', '13884454']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5619765269182784
- 35 USC 102 Novelty (BERT): 0.6109460319213312
- Combined Prediction Score: 0.5668734774185836
- Mean Citation Score: 258.493384
- Max Citation Score: 524.30566
- Similarity Product: 508.0741757807231

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test