PATENT CLAIM ANALYSIS

Application Number: 16212287
Application Type: Utility
Filing Date: 2018-12
Publication Date: 2019-04
Patent Classification: ["435", "189000"]

Abstract:
The present invention relates to novel mutants with cyclase activity and use thereof in a method for biocatalytic cyclization of terpenes, such as in particular for the production of isopulegol by cyclization of citronellal; a method for the preparation of menthol and methods for the biocatalytic conversion of further compounds with structural motifs similar to terpene.

Claim (Index 2):
A method for enzymatic or biocatalytic conversions of compounds of general formula IV in which \u201ca\u201d, \u201cb\u201d, \u201cc\u201d and \u201cd\u201d, in each case independently of one another, represent a single or double C\u2014C bond, with the proviso that cumulative double bonds are excluded; and with the following provisos: R 1  is defined as:\n (1) when \u201ca\u201d is a double bond:\n R 1  is selected from the group consisting of:\n oxo (\u2550O), and \n CH\u2014(CH 2 ) n \u2014Z, \n \u2003in which n is 0, 1 or 2 and \n \u2003Z is OH, CHO; C(O)C 1 -C 4 -alkyl; COOH, C(CH 2 )\u2014CH\u2550CH 2 ; \n \u2003C(OH)(CH 3 )\u2014CH\u2550CH 2 ; C(CH 3 )\u2550CH\u2014CH\u2550CH 2 ; or a radical of the formula C(CH 3 )\u2550CH\u2014CH 2 Y \n \u2003in which \n \u2003Y is OH, CH 2 OH, COOH, or CH 2 C(O)CH 3 ; or \n \n \n (2) when \u201ca\u201d is a single bond:\n R 1  is selected from the group consisting of:\n CH 3 ; CHO; CH 2 CH 2 OH; CH\u2550CH 2 ; CH 2 C(O)OH; CH 2 CHO; and \n C 3 H 6 CH(CH 3 )CHO; \n \n \n and, when \u201ca\u201d is a double bond, it has E or Z configuration; \n R 2  and R 3  are defined as:\n (1) when \u201ca\u201d and \u201cb\u201d are each a single bond:\n R 2  and R 3  independently of one another are H, C 1 -C 4 -alkyl, or OH, or R 2  and R 3  together are a methylene (\u2550CH 2 ) or oxo (\u2550O) group; or \n \n (2) when \u201ca\u201d or \u201cb\u201d is a double bond, one of the radicals R 2  and R 3  is absent and the other of the two radicals is H, C 1 -C 4 -alkyl, or OH; \n R 4  is H or hydroxy-C 1 -C 4 -alkyl; R 5  and R 6  are defined as:\n (1) when \u201cc\u201d is a single bond:\n R 5  and R 6  are each H, or R 5  and R 6  together are an oxo (\u2550O) group; or \n \n (2) when \u201cc\u201d is a double bond, one of the radicals R 5  and R 6  is absent and the other of the two radicals is H; \n R 7 , R 8 , and R 9  are defined as:\n (1) when \u201cd\u201d is a single bond:\n two of the radicals R 7 , R 8  and R 9  in each case independently of one another are H or C 1 -C 4 -alkyl, and the other of the radicals is OH; or \n \n (2) when \u201cd\u201d is a double bond, one of the radicals R 7 , R 8  and R 9  is absent and the other of the two radicals in each case independently of one another are H or C 1 -C 4 -alkyl; \n R 10  is H or hydroxy-C 1 -C 6 -alkyl or mono- or polyunsaturated C 2 -C 6 -alkenyl; comprising reacting a compound of the formula IV in stereoisomerically pure form, or a stereoisomer mixture thereof: a) using an enzyme mutant with cyclase activity which is a mutant of a wild-type enzyme comprising the amino acid sequence of SEQ ID NO: 209 with a mutation at a position corresponding to position F445 of the amino acid sequence of SEQ ID NO: 209, wherein up to 10% of the amino acid residues in said enzyme mutant are altered relative to SEQ ID NO: 209 by deletion, insertion, substitution, addition, inversion, or a combination thereof; or b) in the presence of a microorganism expressing said enzyme mutant, wherein the microorganism comprises:\n i) at least one nucleic acid sequence coding for said enzyme mutant;\n ii) at least one expression cassette comprising said at least one nucleic acid sequence; or \n iii) at least one vector comprising, under the control of at least one regulatory element, said at least one nucleic acid sequence or said at least one expression cassette.

Metadata:
- Claim Count in Document: 12.0
- Percentile: 98.0
- Lexical Diversity: 1.40476
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13297798', '15234529', '14560263', '13798365', '15514706']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5820978521870457
- 35 USC 102 Novelty (BERT): 0.6101750986927768
- Combined Prediction Score: 0.5849055768376188
- Mean Citation Score: 361.44513
- Max Citation Score: 522.1857
- Similarity Product: 518.3632141842128

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test