PATENT CLAIM ANALYSIS

Application Number: 15904548
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-11
Patent Classification: ["800", "008000"]

Abstract:
This invention entails a method to make a Tra-2 RNAi kernel sequence, and uses for this kernel sequence. The method includes the steps of amplifying an RNA recognition motive (RRM) DNA sequence from a  Culcinae  mosquito species, to obtain a first RRM DNA sequence of 240 base pairs; then reversely connecting the first RRM DNA sequence with a second RRM DNA sequence via an intron or a linker DNA sequence, in such a way that the second RRM DNA sequence forms an inverted sequence to the first RRM DNA sequence. Transcription of the two DNA sequences produces single strands of mRNA that can bind together to form a double strand hairpin mRNA structure. Other steps include inserting a tetracycline repressible transactivator, and an insect spermatogenesis promoter.

Claim (Index 10):
A method for increasing genetic maleness offspring in a  Culicinae  mosquito population, comprising the steps of: producing a Tra-2 RNAi construct, which method comprises the steps of:\n a) creating an RNAi kernel sequence by\n i) amplifying an RNA recognition motive (RRM) DNA sequence from a  Culcinae  sequence selected from the group consisting of  Aedes aldopictus, Aedus aegypti, Aedes polynesiensis  and  Culex quinquefasciatus,  to obtain a first RRM DNA sequence of 240 base pairs, \n ii) amplifying an RNA recognition motive (RRM) DNA sequence from a  Culcinae  sequence selected from the group consisting of  Aedes aldopictus, Aedus aegypti, Aedes polynesiensis  and  Culex quinquefasciatus,  to obtain a second RRM DNA sequence of 240 base pairs, \n iii) reversely connecting the first RRM DNA sequence with the second RRM DNA sequence via an intron or a linker DNA sequence, which intron or linker DNA sequence is connected to the end of the first RRM DNA sequence and the beginning of the second RRM DNA sequence, so that the second RRM DNA sequence forms an inverted sequence to the first RRM DNA sequence, \n wherein the transcription of the first RRM DNA sequence and the second RRM DNA sequences produces single strands of mRNA with complementary sequences exposed at ends of the strands of mRNA, which complementary sequences are of sufficient length so as to be capable of binding together to form a double strand hairpin mRNA structure having a loop portion, where the loop portion is formed by the transcription product of the intron or linker DNA sequence, \n iv) inserting a tetracycline repressible transactivator operably linked to and controlling expression of the RNAi kernel sequence, and \n v) inserting an insect spermatogenesis promoter operably linked and controlling expression of the tetracycline repressible transactivator, \n \n b) stably transforming a  Culicinae  mosquito with the Tra-2 RNAi DNA construct, \n c) allowing stable expression of the Tra-2 RNAi DNA construct during spermatogenesis in the  Culicinae  mosquito, so as to effect Tra-2 gene knockdown of X(m) chromosome-bearing sperm, \n d) allowing the  Culicinae  mosquito of step ii) to mate and thereby stably pass on the Tra-2 RNAi DNA construct to offspring, resulting in continuous interruption of the development of X(m) chromosome-bearing sperm and thereby effecting genetic male bias in progeny.

Metadata:
- Claim Count in Document: 5.0
- Percentile: 88.0
- Lexical Diversity: 1.7125
- Patent Class: 800.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14356978', '14991825', '11373042', '15632213', '15022759']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.4867783831735374
- 35 USC 102 Novelty (BERT): 0.6287270236256464
- Combined Prediction Score: 0.5009732472187484
- Mean Citation Score: 372.019794
- Max Citation Score: 708.31757
- Similarity Product: 519.2758834002977

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test