PATENT CLAIM ANALYSIS

Application Number: 16035117
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2018-11
Patent Classification: ["435", "007200"]

Abstract:
Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Claim (Index 31):
A method of detecting a stable interaction between a first amino acid sequence and a second amino acid sequence comprising:\n (a) attaching the first amino acid sequence to a third amino acid sequence, and attaching the second amino acid sequence to a fourth amino acid sequence, wherein the third and fourth amino acid sequences are not fragments of a preexisting protein, wherein a stable complex of the third and fourth amino acid sequences emits a detectable bioluminescent signal in the presence of a substrate, wherein the non-covalent interactions between the third and fourth amino acid sequences are insufficient to form a complex of the third and fourth amino acid sequences in the absence of additional stabilizing and/or aggregating forces, and wherein a stable interaction between the first amino acid sequence and the second amino acid sequence provides the additional stabilizing and/or aggregating forces to produce a stable complex of the third and fourth amino acid sequences; (b) placing the first, second, third, and fourth amino acid sequences of step (a) in conditions to allow for stable interactions between the first amino acid sequence and the second amino acid sequence to occur; and (c) detecting the bioluminescent signal emitted, in the presence of a substrate, by the stable complex of the third and fourth amino acid sequences in the presence of a substrate, wherein detection of the bioluminescent signal indicates a stable interaction between the first amino acid sequence and the second amino acid sequence.

Metadata:
- Claim Count in Document: 23.0
- Percentile: 95.0
- Lexical Diversity: 1.25
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14209546', '14209610', '15073249', '15717534', '16023972']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.8037220636422494
- 35 USC 102 Novelty (BERT): 0.6694349224908052
- Combined Prediction Score: 0.790293349527105
- Mean Citation Score: 861.5191780000001
- Max Citation Score: 905.39746
- Similarity Product: 639.6274125580477

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test