PATENT CLAIM ANALYSIS

Application Number: 15754171
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["435", "377000"]

Abstract:
Provided is a preparation method for olfactory ensheathing cells. The method comprises the steps of formulation of a cell medium, collection and pretreatment of tissues, enzymatic digestion, cell culture, cryopreservation, and differentiation culture. The prepared olfactory ensheathing cells can maintain the proliferative capacity for a long time, and still possess the activity of olfactory ensheathing cells after 11 th  passage.

Claim (Index 3):
The method according to  claim 1 , wherein the culture medium for olfactory ensheathing cells is DMEM/DF12 (Gibco) as a basic culture medium, supplemented with the neurotrophic factors comprising 20-60 ng/ml EGF, 20-80 ng/ml FGF, 1-2 ml N2 (100\u00d7), 2-3 ml B27 (50\u00d7) and 0.1 \u03bcg/ml T3 (Sigma) at final concentrations;\n preferably, the culture medium for olfactory ensheathing cells derived from human olfactory mucosa consists of DMEM/DF 12 (Gibco) and the following neurotrophic factors: 20 ng/ml EGF (Pepro Tech), 20 ng/ml FGF (Pepro Tech), 1% N2 (Gibco), 2% B27 (Gibco) and T3 (Sigma).

Metadata:
- Claim Count in Document: 50.0
- Percentile: 88.0
- Lexical Diversity: 1.64286
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['12680604', '15166695', '11305829', '14233087', '13775556']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5365705192239314
- 35 USC 102 Novelty (BERT): 0.4974912957894266
- Combined Prediction Score: 0.5326625968804809
- Mean Citation Score: 198.39857
- Max Citation Score: 220.2932
- Similarity Product: 154.7440998785496

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test