PATENT CLAIM ANALYSIS

Application Number: 16158853
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-06
Patent Classification: ["435", "007100"]

Abstract:
Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

Claim (Index 3):
The method of  claim 1 , wherein the second subsample is all proteins in the sample.

Metadata:
- Claim Count in Document: 26.0
- Percentile: 97.0
- Lexical Diversity: 1.90741
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15293154', '13870710', '14209185', '13896572', '12505859']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6499239531574034
- 35 USC 102 Novelty (BERT): 0.6468644448620354
- Combined Prediction Score: 0.6496180023278666
- Mean Citation Score: 387.850488
- Max Citation Score: 625.2629
- Similarity Product: 597.7045418556154

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test