PATENT CLAIM ANALYSIS

Application Number: 16021892
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-05
Patent Classification: ["435", "071300"]

Abstract:
The present invention provides sequence specific restriction enzymes for site-specific cleavage of RNA, as well as methods of their use.

Claim (Index 3):
A method of inhibiting expression of a target gene in a cell, comprising introducing into the cell a synthetic site-specific RNA endonuclease which comprises:\n (i) an RNA binding protein domain that comprises a variant of the human  Pumilio  1 homology domain, wherein said variant comprises eight 36-mer repeats, wherein each of the eight 36-mer repeats binds to a single nucleotide in an eight-nucleotide target RNA, wherein said eight-nucleotide target RNA comprises at least one cytosine, and wherein said variant comprises all of amino acids 828 to 1176 of SEQ ID NO:149 except for modifications at one or more positions corresponding to:\n (a) positions 863 to 867 of SEQ ID NO:149, \n (b) positions 899 to 903 of SEQ ID NO:149, \n (c) positions 935 to 939 of SEQ ID NO:149, \n (d) positions 971 to 975 of SEQ ID NO:149, \n (e) positions 1007 to 1011 of SEQ ID NO:149, \n (f) positions 1043 to 1047 of SEQ ID NO:149, \n (g) positions 1079 to 1083 of SEQ ID NO:149, and/or \n (h) positions 1122 to 1126 of SEQ ID NO:149, \n wherein said modifications result in the variant comprising, in any combination, SerXXXGlu (SEQ ID NO:1) to bind guanine, (Cys/Ser)XXXGln (SEQ ID NO:66) to bind adenine, AsnXXXGln (SEQ ID NO:3) to bind uracil, and/or SerTyrXXArg (SEQ ID NO:4) to bind cytosine, wherein X is any amino acid;\n (ii) a linker peptide; and \n (iii) a cleavage domain that comprises a PilT N-terminus (PIN) domain of human SMG6, wherein the RNA binding domain is at the amino terminus of the synthetic site-specific RNA endonuclease and the cleavage domain is at the carboxy terminus of the synthetic site-specific RNA endonuclease, and wherein the cleavage domain cleaves upstream and/or downstream of the eight-nucleotide target RNA, and wherein the RNA binding protein domain of the RNA endonuclease is modified to bind mRNA encoding a gene product of the target gene, under conditions whereby cleavage of the mRNA occurs, thereby inhibiting expression of the target gene in the cell.

Metadata:
- Claim Count in Document: 48.0
- Percentile: 94.0
- Lexical Diversity: 1.1
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13805240', '13989676', '14471782', '15415216', '15428762']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5381722979024306
- 35 USC 102 Novelty (BERT): 0.6751956578643388
- Combined Prediction Score: 0.5518746338986215
- Mean Citation Score: 392.367918
- Max Citation Score: 728.8477
- Similarity Product: 636.9054653661133

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test