PATENT CLAIM ANALYSIS

Application Number: 16011091
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["435", "455000"]

Abstract:
The presently disclosed subject matter provides compositions and methods for the expression of CRISPR guide RNAs using the H1 promoter. In particular, compositions and methods are provided for the use of the H1 promoter to express CRISPR guide RNA (gRNA) with altered specificity of the 5′ nucleotide, as well as use of the H1 promoter sequence as a bidirectional promoter to express Cas9 nuclease and the gRNA simultaneously. Compositions and methods are also provided for the expression and regulation of gRNA expression in vivo through the use of RNA ribozymes and regulatable aptazymes.

Claim (Index 65):
A method, the method comprising introducing into a cell a non-naturally occurring CRISPR system comprising a single vector comprising a bidirectional promoter, wherein the bidirectional promoter comprises:\n a) control elements that provide for transcription in one direction of at least one nucleotide sequence encoding a CRISPR system guide RNA (gRNA), wherein the gRNA hybridizes with a target sequence of a nucleic acid; and b) control elements that provide for transcription in the opposite direction of a nucleotide sequence encoding a RNA-directed nuclease, wherein the gRNA targets and hybridizes with the target sequence and directs the RNA-directed nuclease to the nucleic acid molecule, and wherein said system is packaged into a single adeno-associated virus (AAV) particle.

Metadata:
- Claim Count in Document: 182.0
- Percentile: 94.0
- Lexical Diversity: 1.85185
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15864715', '14951240', '15127087', '14971356', '14523799']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5981698050140879
- 35 USC 102 Novelty (BERT): 0.6289809683662383
- Combined Prediction Score: 0.6012509213493029
- Mean Citation Score: 457.564376
- Max Citation Score: 602.3335599999998
- Similarity Product: 553.855110690994

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test