PATENT CLAIM ANALYSIS

Application Number: 15763585
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-11
Patent Classification: ["435", "440000"]

Abstract:
The present invention provides a construction method of cell models for detecting pyrogens, cell models and pyrogen detection kits. By inducing double-bond breakage in the specific positions of genomes using CRISPR/CAS9, performing directed knock-in of TLR4 and CD14-MD2 on two chromosomes in a cell line based on the principle of homologous recombination and repair, and tracing using green fluorescent protein GFP and red fluorescent protein RFP respectively, we successfully construct the TLR4/CD14/MD2 directed knock-in and fluorescence-traceable cell model; upon the exposure to LPS, the release of IL-6 and TNF-a cytokines in the cell model can be detected by ELISA, Western Blot, mass spectrometry and magnetic bead method; and the cell model has good stability and high sensitivity, and the lowest detection limit can reach 0.005 EU/mL, which is far lower than that of the Limulus amebocyte lysate endotoxin test method (0.025 EU/mL).

Claim (Index 13):
A cell model constructed by the construction method of  claim 4 , wherein the cell model is obtained by knocking the TLR4 gene in the first introne of PPR1R12C gene of chromosome 19 and knocking the CD14 and MD2 genes in the first introne of CCR5 gene of chromosome 3 of the cell line HEK 293T.

Metadata:
- Claim Count in Document: 27.0
- Percentile: 90.0
- Lexical Diversity: 1.53922
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['10432236', '15148663', '14343974', '11613866', '13046562']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6236702366140096
- 35 USC 102 Novelty (BERT): 0.5109919201709849
- Combined Prediction Score: 0.6124024049697071
- Mean Citation Score: 247.929142
- Max Citation Score: 255.49191
- Similarity Product: 153.2035069510889

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test