PATENT CLAIM ANALYSIS

Application Number: 16167181
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-03
Patent Classification: ["435", "007100"]

Abstract:
Methods for producing isotopically-labelled peptides are provided. Aspects of the method include: contacting a sample including a metabolically tagged protein with a cleavable probe to produce a probe-protein conjugate; separating the probe-protein conjugate from the sample; digesting the probe-protein conjugate to produce a probe-peptide conjugate; and cleaving a cleavable linker to release an isotopically labelled peptide. The method may further include: identifying a predetermined isotopic pattern in a mass spectrum; determining an amino acid sequence of the isotopically labelled peptide; and identifying the site of protein glycosylation based on the determined amino acid sequence. Also provided are cleavable probes for practicing the subject methods, described by the Formula: A-L-(M-Z) where A is an affinity tag, L is a cleavable linker, M is an isotopic label and Z is a chemoselective tag capable of cross-linking a metabolically tagged protein. Compositions and kits for practicing the subject methods are also provided.

Claim (Index 39):
The kit of  claim 34 , wherein the metabolically tagged protein is a glycosylated protein comprising an azide-tagged sugar.

Metadata:
- Claim Count in Document: 17.0
- Percentile: 97.0
- Lexical Diversity: 1.90909
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14958730', '09839884', '14147446', '10994815', '12376053']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5342026876824165
- 35 USC 102 Novelty (BERT): 0.6498438469047592
- Combined Prediction Score: 0.5457668036046508
- Mean Citation Score: 337.803692
- Max Citation Score: 732.0661
- Similarity Product: 561.277107561934

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test