PATENT CLAIM ANALYSIS

Application Number: 16199681
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-03
Patent Classification: ["435", "325000"]

Abstract:
Methods, kits, compositions, and systems are provided for culturing pluripotent stem cells to produce populations of cells comprising beta-like cells (e.g., pancreatic lineage, glucose-responsive, and/or insulin-producing). In particular, culture conditions are provided that result in the generation of beta-like cells from a starting culture of human pluripotent stem cells.

Claim (Index 28):
The method of  claim 14 , wherein said cluster aggregates are generated by a method, comprising:\n (i) culturing human pluripotent stem cells on an extracellular matrix for 2-4 days in a chemically defined medium, basic fibroblast growth factor, Activin A, BMP4, LY294002; (ii) culturing the cells from step (i) on an extracellular matrix for 2-4 days in the presence of chemically defined insulin, transferrin and selenium (ITS) medium, FGF7, nicotinamide; (iii) culturing the cells from step (ii) on an extracellular matrix for 2 days in the presence of a chemically defined ITS medium, retinoic acid, Noggin, nicotinamide; (iv) culturing the cell of step (iii) for 2 days in the presence of chemically defined ITS medium, EGF, and FGF7; (v) culturing the cells from step (iv) on an extracellular matrix for 6-10 days in the presence of a B27 serum-free medium, IGF I, IGF II, FGF7, insulin, Noggin, nicotinamide, exendin-4, ALK5i II, forskolin, thereby producing cluster aggregates of insulin+glucagon-pancreatic \u03b2-like cells that exhibit in vitro glucose stimulated insulin secretion; and (vi) maintaining the cells of step (v) for 1-50 days by culturing the cells in a suspension culture in the presence of a serum-free medium, ALK5, Forskolin, ZnSO4, T3, B27, heparin, and antioxidant, Warfarin, and a P13 kinase inhibitor.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 98.0
- Lexical Diversity: 1.46341
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15187345', '14858465', '15705896', '14263123', '13492244']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6752280557034442
- 35 USC 102 Novelty (BERT): 0.7145020647234396
- Combined Prediction Score: 0.6791554566054439
- Mean Citation Score: 652.5239560000001
- Max Citation Score: 824.9689999999997
- Similarity Product: 749.1613878892064

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test