PATENT CLAIM ANALYSIS

Application Number: 16063535
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["506", "016000"]

Abstract:
Disclosed herein are methods and compositions for the detection of small RNAs in a sample. The methods and compositions disclosed herein may be used for preparing sequencing libraries of the small RNAs, fragments of RNAs and DNAs.

Claim (Index 15):
A method for preparing a sequencing library for a plurality of sample polynucleotides in a sample, comprising:\n a) ligating a combo adapter to the plurality of sample polynucleotides to produce a plurality of adapter-polynucleotide ligation products, wherein said combo adapter comprises a 5\u2032-proximal segment and a 3\u2032-proximal segment, and wherein at least one of the 5\u2032 proximal segment or 3\u2032 proximal segment comprises a sequencing adapter; b) circularizing said plurality of adapter-polynucleotide ligation products by ligating their 5\u2032 ends to their 3\u2032 ends to produce a plurality of circularized adapter-polynucleotide ligation products; c) hybridizing a first primer comprising a sequence at least partially complementary to the 5\u2032-proximal segment of said combo adapter, to said circularized adapter-polynucleotide ligation products; d) extending said first primer with a polymerase to produce a plurality of monomeric nucleic acids, wherein each of the monomeric nucleic acids is complementary to: at least one sample polynucleotide of the plurality of sample polynucleotides flanked by at least a portion of the sequencing adapter; and e) amplifying said plurality of monomeric nucleic acids using said first primer and a second primer, wherein the sequence of the second primer is at least partially complementary to the 3\u2032-proximal segment of said combo adapter, to produce amplicon(s) comprising the sequencing library.

Metadata:
- Claim Count in Document: 11.0
- Percentile: 94.0
- Lexical Diversity: 1.48148
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14835544', '15287254', '13264122', '14600550', '14367200']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6967134832428283
- 35 USC 102 Novelty (BERT): 0.611540453447529
- Combined Prediction Score: 0.6881961802632984
- Mean Citation Score: 525.9268480000002
- Max Citation Score: 578.5201400000002
- Similarity Product: 502.590047481754

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test