PATENT CLAIM ANALYSIS

Application Number: 15771906
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-09
Patent Classification: ["435", "071100"]

Abstract:
We discovered that recombinant antibody light chains having a murine secretory leader sequence and an SYE motif at the N-terminus are truncated during post-translational processing. This disclosure provides two protein engineering solutions: to alter the SYE amino acid sequence of the Lc N-terminus to other alternatives; or to change the secretory leader peptide sequence. We have shown that both of these solutions are effective for preventing N-terminal light chain truncation.

Claim (Index 2):
A method of preventing N-terminal truncation of an antibody light chain (Lc) polypeptide, the method comprising:\n a. identifying a first antibody Lc polypeptide having an amino acid sequence comprising a first nascent N-terminal sequence shown in SEQ ID NO: 5, wherein the first nascent N-terminal sequence consists essentially of a leader sequence and a mature N-terminal tripeptide; wherein expression of the first antibody Lc polypeptide in a cultured cell results in truncation of the mature N-terminal tripeptide in at least about 3% of first antibody Lc polypeptides expressed in the cultured cell; and b. expressing in a cultured cell a second antibody Lc polypeptide having the same amino acid sequence as the first antibody Lc polypeptide, except that instead of the first nascent N-terminal sequence, the second antibody Lc polypeptide comprises a second nascent N-terminal sequence selected from the group consisting of:\n SEQ ID NO: 6, \n SEQ ID NO: 7, \n SEQ ID NO: 8, \n SEQ ID NO: 9, \n SEQ ID NO: 10, \n SEQ ID NO: 11, \n SEQ ID NO: 12, \n SEQ ID NO: 13, and \n SEQ ID NO: 14; \n wherein the second nascent N-terminal sequence consists essentially of a leader sequence and a mature N-terminal tripeptide; and wherein expression of the second antibody Lc polypeptide in the cultured cell results in no truncation of the mature N-terminal tripeptide; thereby preventing N-terminal truncation of an antibody Lc polypeptide.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 91.0
- Lexical Diversity: 1.33929
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13934570', '12824058', '12163719', '11777777', '13800233']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5976084515179771
- 35 USC 102 Novelty (BERT): 0.5167185917422937
- Combined Prediction Score: 0.5895194655404088
- Mean Citation Score: 274.353972
- Max Citation Score: 290.66144
- Similarity Product: 191.21662571067813

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test