PATENT CLAIM ANALYSIS

Application Number: 15750824
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-10
Patent Classification: ["506", "002000"]

Abstract:
Reagents and methods for the analysis of nucleic acids (e.g. genomic DNA) of circulating microparticles (i.e. microparticles originating from blood) are provided. The methods comprise linking at least two fragments of a target nucleic acid of a circulating microparticle to produce a set of at least two linked fragments of the target nucleic acid. In the methods, fragments of a target nucleic acid may be linked by techniques such as barcoding, partitioning, ligation and/or separate sequencing. The sequencing of a set of linked fragments provides a set of informatically linked sequence reads corresponding to the sequences of fragments from a single microparticle.

Claim (Index 55):
The method of  claim 42 , wherein the method comprises:\n (a) preparing the sample for sequencing comprising:\n (i) contacting the sample with a multimeric barcoding reagent comprising first and second barcode regions linked together, wherein each barcode region comprises a nucleic acid sequence, and \n (ii) appending barcode sequences to each of the at least two fragments of genomic DNA of the circulating microparticle to produce first and second different barcoded target nucleic acid molecules, wherein the first barcoded target nucleic acid molecule comprises the nucleic acid sequence of the first barcode region and the second barcoded target nucleic acid molecule comprises the nucleic acid sequence of the second barcode region; and \n (b) sequencing each of the barcoded target nucleic acid molecules to produce at least two linked sequence reads; and optionally wherein prior to the step of appending barcode sequences to each of the at least two fragments of genomic DNA of the circulating microparticle, the method comprises appending a coupling sequence to each of the fragments of genomic DNA of the circulating microparticle, wherein a barcode sequence is then appended to the coupling sequence of each of the at least two fragments of genomic DNA of the circulating microparticle to produce the first and second different barcoded target nucleic acid molecules.

Metadata:
- Claim Count in Document: 44.0
- Percentile: 88.0
- Lexical Diversity: 1.94915
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15842687', '14990276', '14006971', '14316447', '14947988']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5337668990876204
- 35 USC 102 Novelty (BERT): 0.5357905316743636
- Combined Prediction Score: 0.5339692623462947
- Mean Citation Score: 344.23017
- Max Citation Score: 360.96902
- Similarity Product: 291.5127790969729

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test