PATENT CLAIM ANALYSIS

Application Number: 15972331
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2018-11
Patent Classification: ["435", "006000"]

Abstract:
The invention relates to a method for the detection of oligonucleotides using anion exchange high performance liquid chromatography. Fluorescently labelled peptide nucleic acid oligomers, complementary to the oligonucleotide are hybridized to the oligonucleotides. Anion exchange high performance liquid chromatography is then performed and the hybridized moieties detected and quantitated. The invention also relates to a method for the simultaneous detection of both strands of an oligonucleotide in parallel from one sample, and a kit for use in qualitative and quantitative detection of one or two strands of an oligonucleotide.

Claim (Index 1):
A method for detecting an oligonucleotide and its oligonucleotide metabolites, wherein the oligonucleotide is an RNA oligonucleotide selected from the group consisting of short interfering RNAs (siRNAs) and microRNAs (miRNAs), comprising the steps of\n (a) selecting a biological sample containing or suspected of containing said RNA oligonucleotide, wherein the sample is tissue lysate or plasma, (b) combining said biological sample with a fluorescently labeled peptide nucleic acid (PNA) probe which is fully complementary to at least 10 nucleotides of said RNA oligonucleotide thereby forming hybridized moieties between said RNA oligonucleotide sequence and said PNA probe, (c) separating said hybridized moieties from unhybridized moieties by anion exchange high performance liquid chromatography (HPLC) under conditions where unhybridized PNA probe elutes in the void volume, and (d) quantitatively detecting said hybridized moieties by fluorescence spectroscopy.

Metadata:
- Claim Count in Document: 22.0
- Percentile: 93.0
- Lexical Diversity: 1.75926
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15554456', '11100897', '12476193', '14648369', '10568134']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5893477182706516
- 35 USC 102 Novelty (BERT): 0.4868219355149204
- Combined Prediction Score: 0.5790951399950784
- Mean Citation Score: 203.036136
- Max Citation Score: 228.8201
- Similarity Product: 175.2472489728034

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test