PATENT CLAIM ANALYSIS

Application Number: 15912075
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-12
Patent Classification: ["435", "208000"]

Abstract:
In one embodiment, the invention provides a method of purifying recombinant alpha-galactosidase A. The method includes obtaining a lysate from cells recombinantly expressing alpha-galactosidase A grown in a cell culture medium having non-precipitating phosphate; contacting said lysate with a first chromatography media that binds α-D-mannopyranosyl or α-D-glucopyranosyl; eluting alpha-galactosidase A from the first chromatography media to generate a first eluate having alpha-galactosidase A, wherein said eluting includes at least one elution pause between 4 and 16 hours; contacting the first eluate with a second chromatography media that binds galactose binding proteins; and eluting alpha-galactosidase A from said second chromatography media to generate a second eluate containing said recombinant alpha-galactosidase A.

Claim (Index 20):
A method of purifying recombinant human alpha-galactosidase A, said method comprising:\n a.) obtaining a lysate from cells recombinantly expressing alpha-galactosidase A grown in a cell culture medium comprising non-precipitating sodium hexametaphosphate as a phosphate source; b.) contacting the lysate with a chromatography media, wherein said chromatography media comprises Concanavalin A; and c.) eluting alpha-galactosidase A from said chromatography media to generate a eluate comprising alpha-galactosidase A, wherein said eluting comprises at least one elution pause of about 10-14 hours.

Metadata:
- Claim Count in Document: 26.0
- Percentile: 90.0
- Lexical Diversity: 1.90323
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14732662', '15545194', '13188907', '14416539', '14573907']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5532826527184116
- 35 USC 102 Novelty (BERT): 0.7097633007039686
- Combined Prediction Score: 0.5689307175169673
- Mean Citation Score: 332.94936
- Max Citation Score: 791.4735
- Similarity Product: 743.2337239751814

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test