PATENT CLAIM ANALYSIS

Application Number: 15993080
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2018-11
Patent Classification: ["435", "061000"]

Abstract:
Disclosed is a high-throughput sequencing method for methylated CpG island in trace DNA, comprising the steps of: 1) treating a DNA sample with bisulfite; 2) adding the treated sample obtained in step 1) to a PCR system containing a primer A for linear amplification; 3) adding an exonuclease having single-stranded DNA cleaving activity to the PCR product in step 2) and inactivating the exonuclease after the reaction; 4) adding the product in step 3) to a PCR system containing a primer B for linear amplification; 5) adding the product in step 4) to a PCR system containing the corresponding adapter primer C and adapter primer D for amplification; and 6) purifying the PCR product in step 5) to obtain a DNA library with a specific length and carrying out the sequencing.

Claim (Index 4):
The method for high-throughput sequencing of methylated CpG island in trace DNA according to  claim 1 , wherein the 3\u2032 sequence of the primer B includes a relatively random annealing sequence having 5-15 bases in length, this sequence contains 0-15 bases C, 0-15 bases D, and 0-7 CpG

Metadata:
- Claim Count in Document: 1.0
- Percentile: 93.0
- Lexical Diversity: 2.23881
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['14392322', '14902509', '14119266', '11071864', '12724416']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6413358923649323
- 35 USC 102 Novelty (BERT): 0.5360551515451274
- Combined Prediction Score: 0.6308078182829517
- Mean Citation Score: 304.92253
- Max Citation Score: 382.28903
- Similarity Product: 311.2631172816004

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test