PATENT CLAIM ANALYSIS

Application Number: 15914356
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2019-01
Patent Classification: ["702", "019000"]

Abstract:
This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

Claim (Index 1):
A method of unambiguously detecting any unique combination of presence or absence of at least five polynucleotide analytes in a plurality of droplets, the method comprising:\n (a) providing a sample comprising, or potentially comprising, at least one of said at least five polynucleotide analytes; (b) forming a mixture of said sample and at least five hybridization probes, each comprising one or more fluorophores selected from at most four fluorophores; (c) partitioning said mixture into said plurality of droplets; (d) exciting said fluorophores to generate one or more signals if one or more of said at least five polynucleotide analytes is present in said plurality of droplets, wherein said one or more signals comprise at least one signal generated by excitement of said one or more fluorophores; and (e) measuring said one or more signals to generate a cumulative measurement, wherein said cumulative measurement corresponds to the presence of a unique combination of presence or absence of said at least five polynucleotide analytes in said sample, thereby detecting the presence or absence of said at least five polynucleotide analytes, in any unique combination of presence or absence; wherein the method does not require any step of immobilization of said at least five polynucleotide analytes or mass spectrometry.

Metadata:
- Claim Count in Document: 29.0
- Percentile: 90.0
- Lexical Diversity: 1.77612
- Patent Class: 702.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14451876', '15623974', '13702860', '15675048', '09956857']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.1839725264363346
- 35 USC 102 Novelty (BERT): 0.5333368065819858
- Combined Prediction Score: 0.2189089544508997
- Mean Citation Score: 224.785846
- Max Citation Score: 378.97086
- Similarity Product: 278.1570995952988

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test