PATENT CLAIM ANALYSIS

Application Number: 15969686
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2018-10
Patent Classification: ["435", "325000"]

Abstract:
High efficiency methods for producing retinal pigment epithelial cells from induced pluripotent stem cells (iPSCs) are disclosed herein. The iPSCs are produced from somatic cells, including retinal pigment epithelial (RPE) cells, such as fetal RPE stem cells. In some embodiments, the iPSC include a tyrosinase promoter operably linked to a marker. Methods are disclosed for using the RPE cells, such as for treatment. Methods for screening for agents that affect RPE differentiation are also disclosed.

Claim (Index 1):
A method for producing human retinal pigment epithelial (RPE) cells, comprising:\n producing embryoid bodies from human induced pluripotent stem cells; culturing the embryoid bodies in a first medium comprising two Wnt pathway inhibitor, and a Nodal pathway inhibitor; plating the embryoid bodies on a matrigel coated tissue culture substrate in a second medium that (a) does not comprise basic fibroblast growth factor (bFGF) (b) comprises a basic fibroblast growth factor (bFGF) inhibitor, the two Wnt pathway inhibitors, and the Nodal pathway inhibitor; (c) comprises 20 to 90 ng of Noggin; and (d) comprises 1 to 3% knock out serum replacement to form differentiating RPE cells; culturing the differentiating RPE cells in a third medium comprising ACTIVIN A and WNT3a; then culturing the cells in RPE cell medium comprising 5% fetal serum, a canonical WNT inhibitor, a non-canonical WNT inducer, and inhibitors of the Sonic and FGF pathway, thereby producing human RPE cells.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 93.0
- Lexical Diversity: 1.72549
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15028618', '15143141', '14712633', '13055708', '12450943']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5830470331594841
- 35 USC 102 Novelty (BERT): 0.5305143813831937
- Combined Prediction Score: 0.5777937679818551
- Mean Citation Score: 315.629002
- Max Citation Score: 353.2595
- Similarity Product: 290.03800218039754

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test