PATENT CLAIM ANALYSIS

Application Number: 16125141
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-03
Patent Classification: ["514", "014100"]

Abstract:
Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH 4 + ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

Claim (Index 76):
A composition comprising recombinant Von Willebrand Factor (rVWF) having a specific ristocetin cofactor activity of at least 40 mU/\u03bcg, wherein the composition is prepared by a method comprising the steps of:\n (a) providing a basal cell culture media; (b) supplementing the basal cell culture media with copper to provide a final copper concentration of at least 2.4 \u03bcg/L; (c) providing one or more cells comprising a nucleic acid encoding a rVWF protein; (d) culturing the one or more cells in the copper supplemented cell culture media such that rVWF is expressed and excreted from the cells into a culture supernatant and the NH4+ content of the cell culture supernatant is maintained at a concentration below 4 mM or below 10 mM; and (e) recovering at least a portion of the culture supernatant, wherein at least 20% of the rVWF in the composition is present in a high molecular weight VWF multimer of more than 10 dimers.

Metadata:
- Claim Count in Document: 80.0
- Percentile: 97.0
- Lexical Diversity: 1.78947
- Patent Class: 514.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13179386', '15201125', '13179399', '15591022', '13622939']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.802229169412368
- 35 USC 102 Novelty (BERT): 0.6646220194002859
- Combined Prediction Score: 0.7884684544111599
- Mean Citation Score: 550.3066359999998
- Max Citation Score: 645.89233
- Similarity Product: 630.6132096716888

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test