PATENT CLAIM ANALYSIS

Application Number: 15878282
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-05
Patent Classification: ["435", "091200"]

Abstract:
Techniques, systems, and devices are disclosed for non-thermal cycling of polymerase chain reaction (PCR). In one aspect, a method for cycling PCR includes receiving an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid in a fluid chamber having a first electrode and a second electrode, applying an electric field across the first and the second electrodes to generate a first pH level of the electrolytic fluid to denature the double-stranded nucleic acid to at least partial single strands, and applying a second electric field across the first and second electrodes to produce a second pH level of the electrolytic fluid, in which the second pH level enables binding of a polymerase enzyme and a primer with a corresponding segment of the single strands.

Claim (Index 1):
A device for cycling polymerase chain reaction (PCR), comprising:\n a substrate that is electrically insulating; a channel formed of an electrically insulative material on the substrate and structured to carry an electrolytic fluid including ions, primers, polymerase enzymes, nucleotides, and a double-stranded nucleic acid; a first electrode pair and a second electrode pair located in the channel in contact with the electrolytic surface and spaced apart along the channel; and a circuit electrically coupled to the first and second electrode pairs to apply a first electric field across the first electrode pair to generate a first pH level of the electrolytic fluid, wherein the double-stranded nucleic acid is capable of denaturation to at least partial single strands at the first pH level, and to apply a second electric field across the second electrode pair to generate a second pH level of the electrolytic fluid, wherein the second pH level enables at least one of binding of a polymerase enzyme and a primer with a corresponding segment of the at least partial single strands or synthesis of new complementary nucleic acid strands from the nucleotides using the at least partial single strands.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 86.0
- Lexical Diversity: 2.1194
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15224873', '13830397', '15018111', '11128301', '12321825']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5133739923619094
- 35 USC 102 Novelty (BERT): 0.5375930964504009
- Combined Prediction Score: 0.5157959027707586
- Mean Citation Score: 238.380048
- Max Citation Score: 369.18042
- Similarity Product: 340.93580788699865

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test