PATENT CLAIM ANALYSIS

Application Number: 15766716
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-11
Patent Classification: ["435", "194000"]

Abstract:
The present invention is directed to a polymerase activity assay that produces a strong optical signal when a primer-template complex is extended to full-length product. The assay uses Cy3 as the molecular beacon and Iowa Black® RQ as the quencher. The signal-to-noise-ratio (STNR) of this donor-quencher pairing is ˜200-fold over background, which is considerably better than other donor-quencher pairs (STNRs ˜10-20-fold). The STNR allows for solution-based monitoring of polymerase activity. Because the sensor functions via Watson-Crick base pairing, the polymerase activity assay may also be used to evolve polymerases to accept xeno nucleic acids as substrates.

Claim (Index 21):
The method of  claim 1 , wherein a hydrophilic microfluidic device emulsifies the water/oil droplet in a bulk aqueous phase to generate a water/oil/water droplet.

Metadata:
- Claim Count in Document: 30.0
- Percentile: 91.0
- Lexical Diversity: 1.44595
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14395429', '13695377', '14872814', '15263476', '14820072']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6627733277206149
- 35 USC 102 Novelty (BERT): 0.5282434205854253
- Combined Prediction Score: 0.6493203370070959
- Mean Citation Score: 312.2629080000001
- Max Citation Score: 336.62045
- Similarity Product: 169.99316513172985

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test