PATENT CLAIM ANALYSIS

Application Number: 15898286
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2019-01
Patent Classification: ["424", "093200"]

Abstract:
This invention provides a method for obtaining a recombinant antibody with a desired function, comprising: (a) providing a population of anti-body-forming cells suspected of containing at least one cell capable of producing an antibody exhibiting the desired function; (b) generating one or more transcriptionally active recombinant linear polynucleotides from the antibody-forming cells obtained in step (a) wherein each transcriptionally active recombinant linear polynucleotide comprises a polynucleotide sequence encoding a variable domain of an antibody produced by an antibody-forming cell obtained in step (a) and one or more transcription regulatory elements; (c) expressing a recombinant antibody using one or more of the transcriptionally active recombinant linear polynucleotides generated in step (b); (d) screening the recombinant antibody produced by step (c) for the desired function; and (e) optionally repeating steps (b), (c) and (d) to identify a recombinant antibody exhibiting the desired function.

Claim (Index 1):
A method for obtaining a recombinant antibody with a desired binding to a selected antigen, comprising:\n (a) providing a population of antibody-forming lymphocyte cells containing at least one cell capable of producing an antibody comprising a heavy chain and a light chain exhibiting the desired binding to the selected antigen;\n (i) screening the population of antibody-forming lymphocyte cells for antibodies that bind to the selected antigen in a first screening step prior to step (b) to identify the antibody-forming cell or a population of antibody-forming cells producing the antibody exhibiting the desired binding to the selected antigen; \n (ii) isolating the antibody-forming cell or the population of antibody-forming cells that produce the antibody exhibiting the desired binding from the population of antibody-forming cells; \n wherein screening (i) and isolating (ii) are carried out simultaneously; \n (b) generating one or more transcriptionally active recombinant linear polynucleotides encoding a light chain or a fragment thereof and one or more transcriptionally active recombinant linear polynucleotides encoding a heavy chain or a fragment thereof for each recombinant antibody from the isolated antibody-forming cell or the population of antibody-forming cells obtained in step (a)(ii) from the antibody-forming cell or the population of antibody-forming cells;\n wherein each transcriptionally active recombinant linear polynucleotide comprises a polynucleotide sequence encoding a variable domain of the antibody produced by the isolated antibody-forming cell or the population of antibody-forming cells obtained in step (a)(ii), a polynucleotide sequence encoding one or more constant domain and one or more transcription regulatory elements; \n wherein each transcriptionally active recombinant linear polynucleotide is not capable of autonomous replication; \n wherein each transcriptionally active recombinant linear polynucleotide is generated by: \n (i) providing the first polynucleotide sequence, the second polynucleotide sequence, and the one or more transcription regulatory elements; \n (ii) fusing the first polynucleotide sequence and the second polynucleotide sequence in a first polymerase chain reaction; and \n (iii) fusing the one or more transcription regulatory elements to the first polynucleotide sequence and/or the second polynucleotide sequence in a second polymerase chain reaction; (c) expressing the recombinant antibody comprising a heavy chain and a light chain from the one or more of the transcriptionally active recombinant linear polynucleotides generated in step (b) by transiently transfecting the one or more of the transcriptionally active recombinant linear polynucleotides into the host cells, growing the host cells and isolating the recombinant antibody from the host cells; (d) screening the recombinant antibody produced by step (c) for the desired binding to the selected antigen in a further screening step; and (e) optionally repeating steps (b), (c) and (d) to identify the recombinant antibody exhibiting the desired binding to the selected antigen.

Metadata:
- Claim Count in Document: 20.0
- Percentile: 88.0
- Lexical Diversity: 2.33784
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['13203182', '14734953', '14414981', '10226949', '15159705']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7122664816057691
- 35 USC 102 Novelty (BERT): 0.6252246533407327
- Combined Prediction Score: 0.7035622987792655
- Mean Citation Score: 392.350098
- Max Citation Score: 619.9951
- Similarity Product: 501.2109340339184

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test