PATENT CLAIM ANALYSIS

Application Number: 15864144
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-05
Patent Classification: ["506", "009000"]

Abstract:
Methods for preparation of cells for analysis of biomarkers are disclosed. In one aspect, the method for preparation of cells for analysis of biomarkers includes contacting a sample that contains a population of cells with at least one modulating substance at a first temperature, thereby producing a modulated cell population; contacting the modulated cell population with at least one antibody that is directed to a cell surface biomarker at a second temperature that is lower than the first temperature, thereby producing an extracellularly stained cell population; and contacting the extracellularly stained cell population with one or more reagents that fixes and permeabilizes the cells, thereby producing a fixed and permeabilized cell population.

Claim (Index 1):
A combination of antibodies directed to cellular biomarkers for identification of cells that have abnormally high signaling activities comprising:\n a combination of antibodies, wherein the antibodies are directed to at least three biomarkers selected from CD8a-144Nd, CD4-145Nd, CD20-147Sm, CD16-Nd148, CD45-154Sm, CD11c-159Tb, CD14-160Gd, CD33-166Er, CD24-168Er, CD3-170Er, CD66-171Yb, CD56-175Lu, CD27-152Sm, CD45RA-153Eu, IgD-161Dy, CD38-167Er, HLA-DR-174Yb, CD25-176Yb, IL3R/CD123-151Eu, CD19-156Gd, CD34-173Yb, CD117-169Tm, IL7R/CD127-158Gd, pp38 MAPK-157Gd, total IKB-163Dy, pSTAT3-164Dy, pSTAT1-169Tm, pSTAT5-172Yb, and pPLCy2-173Yb; wherein the combination of antibodies is configured to identify cells to predict a cause of relapse, guide therapy, and/or predict disease outcome.

Metadata:
- Claim Count in Document: 20.0
- Percentile: 86.0
- Lexical Diversity: 2.18182
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14073692', '12125763', '10898734', '12778847', '13098912']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5420003523590375
- 35 USC 102 Novelty (BERT): 0.5188603954862633
- Combined Prediction Score: 0.5396863566717601
- Mean Citation Score: 262.59957799999995
- Max Citation Score: 277.22623
- Similarity Product: 178.5502308964395

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test