PATENT CLAIM ANALYSIS

Application Number: 15743486
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["514", "034000"]

Abstract:
Nanoassembled complexes of nucleic acids, avidin and biotinylated compounds functionalized to make them suitable for delivering and releasing drugs are described. These nanoassembled complexes consist of central poly-avidin nucleus, i.e. nucleus formed from the self-assembling of tetrameric avidin units on oligonucleotide sequences of nucleic acids, on which biotinylated compounds are further auto-assembled through high-affinity interactions between the avidin of the central nucleus and the biotin of the biotinylated compounds. The biotinylated compounds are functionalized so as to bind drugs with a reversible bond, allowing the delivery thereof and a controlled release.

Claim (Index 1):
Nanoassembled complexes comprising a nucleus consisting of an oligonucleotide sequence and one or more avidin units auto-assembled by means of high-affinity interactions between avidin and one or more nucleobases of the oligonucleotide sequence, and biotinylated compounds assembled on biotin binding sites on the avidin of the nucleus by high-affinity interactions between avidin and biotin represented by the general formula (I)\n NB n Av y (BC) z \u2003\u2003 (I) \n wherein:\n NB are the single nucleobases of the oligonucleotide sequence of a single or double stranded nucleic acid and n is a number higher than 16 and up to 100,000; \n Av is a tetrameric avidin unit and y is an integer higher than or equal to 1 and being in relation to n it is in a range comprised from (0.0001)\u2022n to (0.0454)\u2022n with the proviso that if (0.0001-0.0454)\u2022n is less than 1, y is equal to 1; \n BC are biotinylated compounds selected from compounds of formula: \n B\u2014X a -PA b  (II) where B is a biotin residue, X is a linker having at least two functionalizable residues and PA is a polymeric unit of a hydrophilic polymer having at least two functionalizable residues, one of which binds the biotin residue B with a covalent bond either directly or through the linker X by means of the carboxylic functional group thereof and the second is free or protected with protective groups or binds fluorophores or targeting molecules, a is a number comprised from 0 to 10, b is a number comprised from 1 to 20; \n B\u2014X a -PA b  (III) where B is a biotin residue, X is a linker having at least two functionalizable residues and PA is a polymeric unit of a hydrophilic polymer having at least two functionalizable residues, one of which binds the biotin residue B with a covalent bond either directly or through the linker X by means of the carboxylic functional group thereof and the second is functionalized with a hydrazine residue selected from \u2014R\u2014C(\u2550O)\u2014NHNH 2 , \u2014R\u2014O\u2014C(\u2550O)\u2014NHNH 2 , \u2014R\u2014NH\u2014(C\u2550O)\u2014NHNH 2 \u2014R\u2014NH\u2014(C\u2550S)\u2014NHNH 2  e \u2014R\u2014(C 6 H 4 )\u2014NHNH 2  where R is a linear or branched C 1 -C 10  alkyl residue, a is a number comprised from 0 to 10, b is a number comprised from 1 to 20; \n B\u2014X a  (IV) where B is a biotin residue, X is a linker having at least two functionalizable residues, one of which binds the biotin residue B with a covalent bond directly by means of the carboxylic functional group thereof and the second is functionalized with a hydrazine residue selected from \u2014R\u2014C(\u2550O)\u2014NHNH 2 , \u2014R\u2014O\u2014C(\u2550O)\u2014NHNH 2 , \u2014R\u2014NH\u2014(C\u2550O)\u2014NHNH 2 \u2014R\u2014NH\u2014(C\u2550S)\u2014NHNH 2  and \u2014R\u2014(C 6 H 4 )\u2014NHNH 2  where R is a linear or branched C 1 -C 10  alkyl residue, a is a number in the range comprised from 1 to 10, \n z is an integer higher than or equal to 1 and being in relation toy it is in a range comprised from (0.02)\u2022y to (4)\u2022y with the proviso that if (0.02-4)\u2022y is less than 1, z is equal to 1, with the proviso that the biotin binding sites on avidin of the NB n Av y  nucleus are saturated by at least 5% with biotinylated compounds of formula B\u2014X a -PA b  (II) and/or (III), and wherein the biotinylated compounds of formula (III) and/or (IV) are conjugated with a hydrazone bond with bioactive molecules having at least one carbonyl group.

Metadata:
- Claim Count in Document: 5.0
- Percentile: 86.0
- Lexical Diversity: 1.67797
- Patent Class: 514.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['12667116', '13804602', '13352268', '12771495', '11787932']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7341149896128452
- 35 USC 102 Novelty (BERT): 0.5217622717743362
- Combined Prediction Score: 0.7128797178289943
- Mean Citation Score: 202.196084
- Max Citation Score: 329.49768
- Similarity Product: 258.2724208002091

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test