PATENT CLAIM ANALYSIS

Application Number: 16021892
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-05
Patent Classification: ["435", "071300"]

Abstract:
The present invention provides sequence specific restriction enzymes for site-specific cleavage of RNA, as well as methods of their use.

Claim (Index 2):
A method of cleaving a target mRNA in a cell, comprising introducing into the cell a synthetic site-specific RNA endonuclease which comprises:\n (i) an RNA binding protein domain that comprises a variant of the human  Pumilio  1 homology domain, wherein said variant comprises eight 36-mer repeats, wherein each of the eight 36-mer repeats binds to a single nucleotide in an eight-nucleotide target RNA, wherein said eight-nucleotide target RNA comprises at least one cytosine, and wherein said variant comprises all of amino acids 828 to 1176 of SEQ ID NO:149 except for modifications at one or more positions corresponding to:\n (a) positions 863 to 867 of SEQ ID NO:149, \n (b) positions 899 to 903 of SEQ ID NO:149, \n (c) positions 935 to 939 of SEQ ID NO:149, \n (d) positions 971 to 975 of SEQ ID NO:149, \n (e) positions 1007 to 1011 of SEQ ID NO:149, \n (f) positions 1043 to 1047 of SEQ ID NO:149, \n (g) positions 1079 to 1083 of SEQ ID NO:149, and/or \n (h) positions 1122 to 1126 of SEQ ID NO:149, \n wherein said modifications result in the variant comprising, in any combination, SerXXXGlu (SEQ ID NO:1) to bind guanine, (Cys/Ser)XXXGln (SEQ ID NO:66) to bind adenine, AsnXXXGln (SEQ ID NO:3) to bind uracil, and/or SerTyrXXArg (SEQ ID NO:4) to bind cytosine, wherein X is any amino acid;\n (ii) a linker peptide; and \n (iii) a cleavage domain that comprises a PilT N-terminus (PIN) domain of human SMG6, wherein the RNA binding domain is at the amino terminus of the synthetic site-specific RNA endonuclease and the cleavage domain is at the carboxy terminus of the synthetic site-specific RNA endonuclease, and wherein the cleavage domain cleaves upstream and/or downstream of the eight-nucleotide target RNA, and-wherein the RNA binding protein domain of the RNA endonuclease is modified to bind the target mRNA, under conditions whereby cleavage of the mRNA occurs, thereby cleaving the target mRNA in the cell.

Metadata:
- Claim Count in Document: 48.0
- Percentile: 94.0
- Lexical Diversity: 1.1
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13805240', '13989676', '14471782', '15415216', '15428762']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5323489466124535
- 35 USC 102 Novelty (BERT): 0.6892917844263132
- Combined Prediction Score: 0.5480432303938395
- Mean Citation Score: 392.367918
- Max Citation Score: 728.8477
- Similarity Product: 679.6362345171094

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test