PATENT CLAIM ANALYSIS

Application Number: 15961542
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "006120"]

Abstract:
Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

Claim (Index 7):
The method according to  claim 6 , wherein step (c) comprises: (i) annealing the adaptor-ligated nucleic acid fragments to beads, each bead annealing with a single adaptor-ligated nucleic acid fragments; (ii) emulsifying the beads in water-in-oil microreactors such that each microreactor contains a single bead; (iii) performing emulsion PCR to amplify adaptor-ligated nucleic acid fragments on the surface of the beads; (iv) selecting/enriching beads to which are attached the amplified adaptor-ligated nucleic acid fragments; (v) placing the beads in wells such that each well comprises a single bead; and generating a pyrophosphate signal.

Metadata:
- Claim Count in Document: 25.0
- Percentile: 91.0
- Lexical Diversity: 1.77778
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15400920', '15288253', '13447871', '15165921', '13972152']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7301533782595911
- 35 USC 102 Novelty (BERT): 0.6265418184887609
- Combined Prediction Score: 0.719792222282508
- Mean Citation Score: 536.793668
- Max Citation Score: 561.5664
- Similarity Product: 533.3408970720292

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test