PATENT CLAIM ANALYSIS

Application Number: 16294864
Application Type: Utility
Filing Date: 2019-03
Publication Date: 2019-07
Patent Classification: ["435", "006000"]

Abstract:
Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.

Claim (Index 98):
A method of detecting two or more nucleic acid targets within an individual cell, the method comprising:\n providing a sample comprising the cell, which cell comprises or is suspected of comprising two or more nucleic acid targets; providing for each nucleic acid target a label probe system comprising two or more identical label probe complexes, each of which comprises a nucleic acid component and one or more labels, wherein the labels of the label probe systems are distinct for each target nucleic acid; providing for each nucleic acid target a capture probe system comprising two or more sets of capture probes, wherein each set of capture probes comprises two or more different capture probes, wherein all capture probes in the capture probe system comprise a T section and an L section, wherein the T section is a nucleic acid sequence complementary to a section on the nucleic acid target and the L section is a nucleic acid sequence complementary to a section on the nucleic acid component of the label probe complex, and wherein the T sections of the different capture probes are complementary to non-overlapping regions of the nucleic acid target, and the L sections of the different capture probes are complementary to non-overlapping regions of the nucleic acid component of the label probe complex; hybridizing, in the cell and in the presence of cellular non-target nucleic acids, the capture probe system to the two or more nucleic acid targets, when present in the cell, thereby providing hybridization of two or more different capture probe sets to a single copy of the nucleic acid target molecules in the presence of cellular non-target nucleic acids; capturing, in the cell and in the presence of cellular non-target nucleic acids, the label probe system to the capture probe system hybridized to the nucleic acid target molecules, wherein the capturing occurs by simultaneously hybridizing the at least two different capture probes in each capture probe set to a single molecule of the nucleic acid component of the label probe complex that is complementary to the L sections of the capture probes, thereby capturing the two or more label probe complexes to the nucleic acid target in the presence of cellular non-target nucleic acids; and detecting a signal from the two or more label probe complexes captured on the nucleic acid targets.

Metadata:
- Claim Count in Document: 59.0
- Percentile: 99.0
- Lexical Diversity: 1.85714
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13278353', '14151716', '11431092', '11543752', '14105080']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6744856704942824
- 35 USC 102 Novelty (BERT): 0.5172620146294549
- Combined Prediction Score: 0.6587633049077997
- Mean Citation Score: 304.67635200000007
- Max Citation Score: 320.12885
- Similarity Product: 245.6569275473893

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test