PATENT CLAIM ANALYSIS

Application Number: 15751792
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-10
Patent Classification: ["530", "388100"]

Abstract:
A method for separating cells capable of producing target antigen-specific monoclonal antibodies (TASMAs) wherein a cell group including antibody-producing cells is immobilized using a reversible crosslinking agent having cell membrane-permeating properties. The immobilized cell group is subjected to cell membrane dissolution using a surface active agent; and the cell group is reacted with a labeling target antigen. In the stained cell group a that has reacted with the labeling target antigen is separated. A method to produce TASMAs by separating mRNA from the cell separated using the method; preparing cDNA and preparing antigen-specific monoclonal antibodies or fragments thereof from the prepared cDNA. Also provided are a method whereby at least one cell capable of producing TASMAs is separated and a method whereby said antibodies can be produced by using the separated cell. Threonine 18 phosphorylated p53 (pT18-p53) and threonine 68 phosphorylated CHK2 (pT68-CHK2) specific monoclonal antibodies are also disclosed.

Claim (Index 6):
A method for producing a monoclonal antibody with specificity toward an antigen of interest, the method comprises the following steps:\n (A) preparing a cDNA by separating mRNA from the at least one cell isolated by the method of any one of  claims 1  to  5  (a cDNA preparing step); and (B) preparing the antigen specific monoclonal antibody or fragment thereof from the cDNA obtained in the cDNA preparing step (a preparing step of the monoclonal antibody specific to an antigen of interest).

Metadata:
- Claim Count in Document: 27.0
- Percentile: 88.0
- Lexical Diversity: 1.96386
- Patent Class: 530.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['13425538', '15042106', '14787478', '10338366', '15361936']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6605267184008319
- 35 USC 102 Novelty (BERT): 0.5161943230453567
- Combined Prediction Score: 0.6460934788652845
- Mean Citation Score: 284.8170320000001
- Max Citation Score: 292.74838
- Similarity Product: 218.7816214899659

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test