PATENT CLAIM ANALYSIS

Application Number: 15862184
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-05
Patent Classification: ["435", "006100"]

Abstract:
Compositions and methods for isolating new variants of known gene sequences are provided. The methods find use in identifying variants, particularly homologs, in complex mixtures. Compositions comprise hybridization baits that hybridize to gene families of interest, particularly agricultural interest, in order to selectively enrich the polynucleotides of interest from complex mixtures. Bait sequences may be specific for a number of genes from distinct gene families of interest and may be designed to cover each gene of interest by at least 2-fold. Thus methods disclosed herein are drawn to an oligonucleotide hybridization gene capture approach for identification of new genes of interest from environmental samples. This approach bypasses the need for labor-intensive microbial strain isolation, permits simultaneous discovery of genes from multiple gene families of interest, and increases the potential to discover genes from low-abundance and unculturable organisms present in complex mixtures of environmental microbes.

Claim (Index 1):
A method for identifying a variant of a gene of interest in a complex sample, comprising:\n a) obtaining a complex sample comprising a variant of a gene of interest; b) preparing DNA from said complex sample for hybridization thereby forming a prepared sample DNA, the prepared sample DNA comprising said variant of said gene of interest; c) mixing said prepared sample DNA with a labeled bait pool comprising polynucleotide sequences complementary to said gene of interest; d) hybridizing the prepared sample DNA to said labeled bait pool under conditions that allow for hybridization of a labeled bait in said labeled bait pool with said variant of said gene of interest to form one or more hybridization complexes, wherein said variant of said gene of interest in the hybridization complexes comprises captured DNA; e) sequencing said captured DNA to determine a sequence read of said variant of said gene of interest; and f) identifying said variant of said gene of interest from said sequence read by performing a sequence similarity search using the sequence read gene sequence against a database of known sequences, said variant of said gene of interest having less than 95% identity to said gene of interest.

Metadata:
- Claim Count in Document: 60.0
- Percentile: 86.0
- Lexical Diversity: 1.85714
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14592473', '13339986', '13957408', '13516615', '13516668']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5124752166850883
- 35 USC 102 Novelty (BERT): 0.57491784304485
- Combined Prediction Score: 0.5187194793210645
- Mean Citation Score: 230.518752
- Max Citation Score: 445.0344
- Similarity Product: 439.0859977682591

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test