PATENT CLAIM ANALYSIS

Application Number: 16006210
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-01
Patent Classification: ["424", "186100"]

Abstract:
Provided herein are methods for the purification of recombinant adeno-associated virus (rAAV) vectors that can be used for gene transfer and specifically for gene therapy or vaccination. Recombinant AAV vectors of the invention are substantially free of in-process impurities, including production components such as cellular nucleic acids, cellular proteins, helper virus, and media components.

Claim (Index 48):
A method for isolating a population of recombinant adeno-associated virus (rAAV) particles from in-process impurities in a feedstream, comprising the steps of:\n (a) subjecting the feedstream containing the rAAV particles to flow-through anionic exchange filtration (b) subjecting the feedstream containing the rAAV particles to TFF (c) (i) contacting the feedstream containing the rAAV particles with an apatite chromatography medium in the presence of polyethylene glycol (PEG), wherein the rAAV particles bind to the apatite chromatography medium; and\n (ii) eluting the rAAV particles bound to the apatite chromatography medium with an elution buffer containing less than 3% (w/v) PEG; \n (d) heat inactivating a helper virus in the feedstream eluted from the apatite chromatography medium (e) (i) contacting the feedstream containing the rAAV particles eluted from the apatite chromatography medium with a hydrophobic interaction chromatography (HIC) medium in a high salt buffer comprising citrate, wherein the rAAV particles and the in-process impurities bind to the HIC medium; and\n (ii) eluting the rAAV particles bound to the HIC medium with a medium salt buffer; \n (f) subjecting the feedstream containing the rAAV particles eluted from the HIC medium to SEC; and (g) subjecting the feedstream containing the rAAV particles eluted from the SEC medium to anionic exchange chromatography.

Metadata:
- Claim Count in Document: 55.0
- Percentile: 94.0
- Lexical Diversity: 1.34783
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13328306', '10016767', '15350409', '10020482', '10851688']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7146986976236104
- 35 USC 102 Novelty (BERT): 0.640946061364157
- Combined Prediction Score: 0.7073234339976651
- Mean Citation Score: 321.508458
- Max Citation Score: 662.2674599999998
- Similarity Product: 559.0143961109053

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test