PATENT CLAIM ANALYSIS

Application Number: 16063639
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-05
Patent Classification: ["435", "114000"]

Abstract:
The invention discloses a method for increasing the yield of L-arginine by knocking out flavin reductases, and belongs to the technical field of amino acid production by microbial fermentation. Genes frd1 and frd2 for encoding hypothetic NADPH-dependent FMN reductase in  Corynebacterium crenatum  SDNN403 are over-expressed in  E. coli  BL21 and are purified to form target proteins Frd181 and Frd188, and functions of the target proteins are identified to obtain a result showing that the proteins Frd181 and Frd188 both are NAD(P)H-dependent flavin reductases producing H 2 O 2 . By taking a genome of the  Corynebacterium crenatum  SDNN403 as a template, frd1 and frd2 gene deletion fragments are obtained by overlap extension PCR; connecting pK18mobsacB to obtain knockout plasmids pK18mobsacB-Δfrd1 and pK18mobsacB-Δfrd2; carrying out electric shock to transform the  Corynebacterium crenatum  SDNN403; and carrying out secondary screening to obtain recombinant strains 403Δfrd1 and 403Δfrd2. Found by flask shaking fermentation, the yield of L-arginine is obviously increased by knocking out the genes frd1 and frd2.

Claim (Index 3):
The recombinant strain of the  Corynebacterium crenatum  of  claim 1 , wherein the recombinant strain of the  Corynebacterium crenatum  is obtained by knocking out NADPH-dependent FMN reductase genes in  Corynebacterium crenatum  CGMCC NO:0890.

Metadata:
- Claim Count in Document: 14.0
- Percentile: 94.0
- Lexical Diversity: 1.72816
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['14891622', '15891564', '15307455', '15876478', '12993215']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5642840963995805
- 35 USC 102 Novelty (BERT): 0.5006731497929622
- Combined Prediction Score: 0.5579230017389187
- Mean Citation Score: 207.173384
- Max Citation Score: 230.70525
- Similarity Product: 166.27117722389102

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test