PATENT CLAIM ANALYSIS

Application Number: 15865574
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["424", "093210"]

Abstract:
The technology described herein relates to methods and kits for directed differentation of primordial NKX2-1+ lung progenitors along proximal differentiation pathways into functional airway epithelial cells and airway organoids (“bronchospheres”) or along distal lineage pathways using modulation of Wnt signaling. Other aspects relate cell lines, methods, assays and kits comprising airway epithelial cells, and assays for diagnosing a disease that affects swelling of the bronchospheres, and/or for assessing genetic lesions and/or drugs for treating the the disease, where the disease is cystic fibrosis. Other aspects relate to personalized medicine and methods of treatment of cystic fibrosis using the airway epithelial cells.

Claim (Index 9):
A method for modulating the cononical Wnt pathway in a population of NKX2-1 lung epithelial progenitors to direct their differentiation along a proximal or dorsal epithelial pathway, the method comprising;\n a. culturing a population of NKX2-1 lung epithelial progenitors in a low-Wnt media for a sufficient amount of time, wherein the low-Wnt media induces the NKX2-1 lung epithelial progenitors to differentiate along a proximal epithelial pathway into airway epithelial organoids comprising cells selected from any of: NKX2-1+/SOX2+ cells, NKX2-1+/P63+ cells or NKX2-1+/P63+/K5 cells; or b. culturing a population of NKX2-1 lung epithelial progenitors in a high Wnt media, or in the presence of a Wnt activator for a sufficient amount of time to allow the NKX2-1 lung epithelial progenitors to differentiate along a distal epithelial pathway into cells selected from any of: NKX2-1+/SOX9+ cells, NKX2-1+/SFTPC+ cells or NKX2-1+/Scgb1a1\u2212 cells.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 86.0
- Lexical Diversity: 1.66176
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15635975', '14371627', '14367061', '15223603', '14417273']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7690865136432011
- 35 USC 102 Novelty (BERT): 0.532709907835968
- Combined Prediction Score: 0.7454488530624778
- Mean Citation Score: 358.088658
- Max Citation Score: 371.3459
- Similarity Product: 278.884108344078

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test