PATENT CLAIM ANALYSIS

Application Number: 15905521
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-06
Patent Classification: ["435", "069100"]

Abstract:
The present invention provides a method of designing an optimized gene which comprises altering a nucleotide sequence of a target protein gene, so that only preferential codons with high frequency of use in human cells are selected and a GC content of not less than 60% is achieved. A gene design method which involves the feature “only preferential codons with high frequency of use are selected and a GC content of not less than 60% is achieved” can be established as a general rule for preparing proteins with high expression level, in order to obtain chemically synthesized genes for proteins capable of high-level expression in eukaryotes.

Claim (Index 48):
A method of producing a  Renilla  luciferase fusion protein, comprising:\n preparing a recombinant expression vector comprising the polynucleotide of  claim 45  under the control of a promoter capable of functioning in a mammalian cell, introducing the recombinant expression vector into a mammalian cell to produce a recombinant mammalian cell, and culturing the recombinant mammalian cell to produce the  Renilla  luciferase fusion protein.

Metadata:
- Claim Count in Document: 17.0
- Percentile: 88.0
- Lexical Diversity: 1.60563
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14848535', '09808898', '10377714', '14188838', '14244953']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5791027376883054
- 35 USC 102 Novelty (BERT): 0.6072380974930351
- Combined Prediction Score: 0.5819162736687784
- Mean Citation Score: 368.943092
- Max Citation Score: 563.46857
- Similarity Product: 474.23828929761527

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test