PATENT CLAIM ANALYSIS

Application Number: 16056343
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-11
Patent Classification: ["435", "006000"]

Abstract:
Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

Claim (Index 1):
A method for assembling a nucleic acid construct comprising:\n (a) generating a plurality of double-stranded nucleic acid cassettes from chemically synthesized oligonucleotides or copies thereof, wherein the cassettes comprise adjacent regions of a nucleic acid construct to be assembled and wherein each of the cassettes overlap with one or more other cassette(s) by at least 50 nucleotides and; (b) simultaneously assembling the plurality of nucleic acid cassettes in vitro in a chew back and repair step utilizing:\n an enzyme having 3\u2032 or 5\u2032 exonuclease activity, \n a DNA polymerase, \n polyethylene glycol (PEG) or a single-stranded binding protein, and a ligase, \n thereby assembling the nucleic acid construct.

Metadata:
- Claim Count in Document: 53.0
- Percentile: 96.0
- Lexical Diversity: 2.11321
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['11635355', '12247126', '14015070', '12783489', '12718911']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5362426750169037
- 35 USC 102 Novelty (BERT): 0.5406012552151767
- Combined Prediction Score: 0.536678533036731
- Mean Citation Score: 248.650448
- Max Citation Score: 377.24207
- Similarity Product: 306.5748616687435

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test