PATENT CLAIM ANALYSIS

Application Number: 15882714
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-09
Patent Classification: ["530", "331000"]

Abstract:
A facile laboratory-based method and kit for use in accordance with the method is disclosed. The method allows for the localisation of biomolecules comprising a conjugatable sulfhydryl group to be localised to the surface of cells, such as red blood cells, as lipid conjugates. The method obviates the need to purify the lipid-conjugated biomolecule before contacting with the cells.

Claim (Index 47):
A kit provided with instructions for preparing a freeze-dried reaction product comprising a water dispersible lipid conjugated biomolecule that spontaneously incorporates into the membranes of cells when an aqueous solution of the freeze-dried reaction product is contacted with the cells, where the kit comprises:\n a quantity of a lipid conjugated maleimide of the structure F-S-L; and a separate volume of a volatile reaction buffer sufficient to dissolve the quantity of lipid conjugated maleimide and a molar excess of the biomolecule to be conjugated, where:\n F \n is\n S \n is\n L is an amide linked diacyl- or dialkyl-glycerophospholipid, \n m is the integer 1, 2 or 3, \n M is a monovalent cation or substituent, \n n is the integer 1, 2 or 3, \n p is the integer 1, 2 or 3, \n q is the integer 3, 4 or 5, \n the volatile reaction buffer consists essentially of 0.05 to 0.3 M amine component in 20 to 60% organic co-solvent at a pH of 5.8 to 7.5 where the amine component is selected from the group consisting of: 4-methylmorpholine formate, 4-ethylmorpholine, triethylamine, trimethylamine, N-methylpiperidine and N-methylpyrrolidone; and the organic co-solvent is selected from the group consisting of: isopropanol, n-propanol, ethanol, methanol, t-butanol, 2-methoxyethanol and trifluoroethanol, and \n the biomolecule comprises a conjugatable sulfhydryl (\u2014SH) group.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 86.0
- Lexical Diversity: 1.45455
- Patent Class: 530.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14241578', '15490471', '13380711', '15101077', '15598505']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6173236960502108
- 35 USC 102 Novelty (BERT): 0.5798869815568707
- Combined Prediction Score: 0.6135800246008768
- Mean Citation Score: 275.677974
- Max Citation Score: 488.02417
- Similarity Product: 392.3115492172188

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test