PATENT CLAIM ANALYSIS

Application Number: 15754171
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["435", "377000"]

Abstract:
Provided is a preparation method for olfactory ensheathing cells. The method comprises the steps of formulation of a cell medium, collection and pretreatment of tissues, enzymatic digestion, cell culture, cryopreservation, and differentiation culture. The prepared olfactory ensheathing cells can maintain the proliferative capacity for a long time, and still possess the activity of olfactory ensheathing cells after 11 th  passage.

Claim (Index 9):
The method according to  claim 1 , wherein the preparation method comprises the following steps:\n Step 1. formulating a culture medium for olfactory ensheathing cell with neurotrophic factors; Step 2. collection of tissues: picking up an appropriate amount of the olfactory mucosa tissues of upper and middle turbinate under local anesthesia; Step 3. pretreatment of tissue blocks: washing the residual blood on the surface of the obtained tissue blocks and processing the tissue blocks into ones in a size of 1 mm 3 ; Step 4. digestion by a combination of two enzymes: collecting the scissor-minced tissue blocks, adding 2 volumes of Collagenase I and a neutral protease, and digesting in water bath at 37\u00b0 C. under vibration; Step 5. primary culture: collecting the single cells obtained by the digestion and placing them in a centrifuge tube, mixing thoroughly with a culture medium for olfactory ensheathing cells, then inoculating in a cell culture flask, and culturing in an incubator at 37\u00b0 C. and 5% CO 2 ; Step 6. passage culture: collecting a culture supernatant of the cells to be passaged when the cells grow adherently to around 90%, filtering and mixing the supernatant with a culture medium for olfactory ensheathing cells in a ratio of 1:3, to formulate a culture medium for the present passage; Step 7. cryopreservation at an ultralow temperature: collecting and filtering a supernatant of cells to be cryopreserved when the cells grow adherently to around 90%, to formulate a cryopreservation medium specific for the olfactory ensheathing cells (consisting of the autologous serum, the cell culture supernatant, DMEM/F12 and DMSO); and cryopreserving according to the routine procedures; Step 8. differentiation culture: adding a formulated culture medium for the differentiation of glial cells (a culture medium for olfactory ensheathing cells: the autologous serum=87:13), culturing for differentiation according to the routine procedures.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 88.0
- Lexical Diversity: 1.64286
- Patent Class: 435.0
- Transitional Phrase Type: closed
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['12680604', '15166695', '11305829', '14233087', '13775556']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6000096498880362
- 35 USC 102 Novelty (BERT): 0.5018014490206837
- Combined Prediction Score: 0.5901888298013009
- Mean Citation Score: 198.39857
- Max Citation Score: 220.2932
- Similarity Product: 145.64995640203958

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test