PATENT CLAIM ANALYSIS

Application Number: 15886829
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-06
Patent Classification: ["435", "174000"]

Abstract:
The present invention relates to fields of continuous directional catalysis of soybean slurry to extract protein and functional oil with nano-magnetic immobilized enzymes. The present invention discloses soybean slurry as raw material and continuous mobile phase, and nano-magnetic immobilized enzymes as stationary phase applied in three-phase magnetic fluidized bed. The present invention provides a method of enzymatic disrupting cell walls by nano-magnetic immobilized enzyme cocktail including cellulose, pectinase and alkaline protease. The method can he used to break down lipoprotein, lipopolysaccharide complex and break lipoprotein film on the surface of lipoprotein to release the lipid. The present invention also provides a method to catalyze phospholipid using nano-magnetic immobilized phospholipase to change the polarity of phospholipids and control liquid emulsification phenomenon. The enzymes disclosed in the present invention do not interfere with each other. The preparation method can be used to catalyze soybean slurry continuously using the nano-magnetic immobilized enzymes to extract protein and functional lipid simultaneously.

Claim (Index 1):
A method for extracting proteins and functional lipid from soybean liquid by directional continuous catalysis with nano magnetic immobilized enzymes, comprising following steps:\n adding the nano magnetic immobilized enzymes in a five-order-three-phase magnetic fluidized bed and taking the nano magnetic immobilized enzymes as a stationary phase; taking a soybean as a mobile phase, and enabling the nano magnetic immobilized enzymes in a five-order-three-phase magnetic fluidized bed reaction system to be steady by adjusting reaction operation parameters and performing directional enzymolysis reaction with corresponding zymolyte; increasing enzymolysis effect by adjusting a sequence and reaction conditions based on different enzymolysis properties of the nano magnetic immobilized enzymes; obtaining a soy protein and functional oils with rich DAG and realizing extraction of proteins and functional lipid from soybean liquid by directional continuous catalysis with nano magnetic immobilized enzymes; wherein the five-order-three-phase fluidized bed reaction system comprises a three-phase (gas-liquid-solid) magnetic fluidized bed, a soybean material tank, a pH regulating tank, and a power plant; wherein the nano magnetic immobilized enzymes comprises nano magnetic immobilized cellulase, nano magnetic immobilized pectinase, nano magnetic immobilized protease, nano magnetic immobilized phospholipase C and nano magnetic immobilized phospholipase A1.

Metadata:
- Claim Count in Document: 13.0
- Percentile: 88.0
- Lexical Diversity: 1.90805
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15163795', '10592450', '11567318', '13502069', '13502105']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.558245309233533
- 35 USC 102 Novelty (BERT): 0.5035495681956172
- Combined Prediction Score: 0.5527757351297414
- Mean Citation Score: 192.021682
- Max Citation Score: 209.79166
- Similarity Product: 120.61325832385064

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test