PATENT CLAIM ANALYSIS

Application Number: 15752994
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["530", "387300"]

Abstract:
A refolding process of Ranibizumab is disclosed wherein the solubilized solution of heavy chain and/or light chain of Ranibizumab treated with refolding buffer under suitable conditions including pH, temperature and incubation period and the pH and temperature shift is performed at suitable interval to obtain high quality and quantity of refolded protein.

Claim (Index 21):
The process for recovering a refolded Ranibizumab as claimed in  claim 1  comprising the steps of:\n a) isolating a light chain and/or heavy chain of Ranibizumab from a bacterial host cell; \n b) solubilizing said light chain and/or heavy chain of Ranibizumab in a first buffer solution comprising a chaotropic agent and/or reducing agent at a pH of about 9; \n c) refolding said solubilized light chain and heavy chain of Ranibizumab in a second buffer solution under suitable conditions having a pH of about 10, temperature at least about 5\u00b0 C. to 10\u00b0 C., for a first incubation period of at least about 4 hours; \n d) performing a shift in the suitable conditions of step (c) to a pH of about 9, temperature at least about 20\u00b0 C. to 25\u00b0 C., for a second incubation period of about 14 hours to obtain refolded Ranibizumab; and \n e) recovering said refolded Ranibizumab.

Metadata:
- Claim Count in Document: 11.0
- Percentile: 88.0
- Lexical Diversity: 1.35
- Patent Class: 530.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['13668182', '14922802', '13634801', '11341013', '14766849']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6409486591980587
- 35 USC 102 Novelty (BERT): 0.521160906344759
- Combined Prediction Score: 0.6289698839127287
- Mean Citation Score: 278.37254
- Max Citation Score: 314.81305
- Similarity Product: 234.4597282550812

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test