PATENT CLAIM ANALYSIS

Application Number: 16010902
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["435", "091200"]

Abstract:
The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.

Claim (Index 21):
The method of  claim 20 , wherein the solid support is prepared by a method comprising polymerizing on the solid support a mixture of:\n (i) a first comonomer which is acrylamide, methacrylamide, hydroxyethyl methacrylate or N-vinyl pyrrolidinone; and (ii) a second comonomer which is a functionalized comonomer selected from acrylamide or acrylate of formula (I):\n H2C\u2550C(H)\u2014C(\u2550O)-A-B\u2014C\u2003\u2003(I); \n or a methacrylate or methacrylamide of formula (II):\n H2C\u2550C(CH3)-C(\u2550O)-A-B\u2014C\u2003\u2003(II); \n \n wherein \n A is NR or O, wherein R is hydrogen or an optionally substituted saturated hydrocarbyl group comprising 1 to 5 carbon atoms; \n B is an optionally substituted alkylene biradical of formula \u2014(CHn)- wherein n is an integer from 1 to 50; and wherein n=2 or more, one or more optionally substituted ethylene biradicals \u2014CH 2 CH 2 \u2014 of said alkylene biradical may be independently replaced by ethenylene and ethynylene moieties; and wherein n=1 or more, one or more methylene biradicals \u2014CH 2 \u2014 may be replaced independently with an optionally substituted mono- or polycyclic hydrocarbon biradical comprising from 4 to 50 carbon atoms, or a corresponding heteromonocyclic or heteropolycyclic biradical wherein at least 1 CH 2  or CH 2  is substituted by an oxygen sulfur or nitrogen atom or an NH group; and \n C of A-B\u2014C of formulas (I) or (II) is a group for reaction with a compound to bind said compound covalently to said hydrogel to form a polymerized product, \n characterized in that polymerization is conducted on and immobilized the polymerized product to a solid support that is not covalently surface-modified.

Metadata:
- Claim Count in Document: 18.0
- Percentile: 94.0
- Lexical Diversity: 1.88889
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15287077', '14738591', '14166670', '13450920', '12223759']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7028870771225483
- 35 USC 102 Novelty (BERT): 0.6484677028744638
- Combined Prediction Score: 0.6974451396977398
- Mean Citation Score: 592.3149380000001
- Max Citation Score: 673.77747
- Similarity Product: 582.3238679766655

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test