PATENT CLAIM ANALYSIS

Application Number: 15888702
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-06
Patent Classification: ["435", "006120"]

Abstract:
Methods for separating and identifying nucleic acids utilize carbon coated magnetic beads. The method teaches that multivalent cations promote binding of single stranded nucleic acids to the beads and that the single stranded nucleic acids can be released with the addition of chelating agents that bind the multivalent cations such as EDTA. The method further teaches that fragile single stranded nucleic acids, such as RNA, can be stored on the surface of the beads. Lastly, the method also teaches that by iteratively adding complimentary DNA oligos, single stranded nucleic acids can be quantified or individually isolated using the carbon coated magnetic beads.

Claim (Index 6):
A method for isolating nucleic acids having a defined sequence from an aqueous solution, the method comprising:\n contacting a composition containing single stranded nucleic acid and double stranded nucleic acid with at least one nucleic acid probe, the nucleic acid probe complementary with amino acids present on at least a portion of the nucleic acid in the aqueous solution, the contacting step occurring for an interval sufficient for the at least one nucleic acid probe to duplex with amino acid sequences present on at least a portion of the nucleic acid present in the solution to produce an admixture composed of duplexed nucleic acid material and non-duplexed nucleic acid material; to the resulting admixture containing duplexed nucleic acid material, adding carbon-coated material, at least one chaotropic salt and short-chain alcohol, the short-chain alcohol present in an amount between 2 vol % and 99 vol %; allowing contact for an interval sufficient to permit association between the carbon coated material and at least a portion of the single stranded nucleic acids present in the admixture; and separating carbon-coated material complexed with the single stranded nucleic acids from liquid supernatant, the liquid supernatant containing complexed double stranded nucleic acid.

Metadata:
- Claim Count in Document: 34.0
- Percentile: 88.0
- Lexical Diversity: 1.92982
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12301985', '12995348', '14238625', '09760085', '13943986']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5780991229377178
- 35 USC 102 Novelty (BERT): 0.4743941840251808
- Combined Prediction Score: 0.5677286290464642
- Mean Citation Score: 196.674188
- Max Citation Score: 206.03911
- Similarity Product: 164.14187824868918

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test