PATENT CLAIM ANALYSIS

Application Number: 16080428
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2019-02
Patent Classification: ["435", "007100"]

Abstract:
Provided are methods of detecting and quantitatively measuring protein-protein interactions between proteins present on the surface of different cells using a sensitive antibody-based assay. Antibodies specific for each target protein are either directly labeled, or are detected by labeled secondary antibodies. The antibodies are labeled with detectable tags that are releasable when the target proteins are within proximity to each other. Also provided are methods for facilitating diagnosis, prognosis, and treatment of cancer using this assay to detect protein-protein interactions between target proteins of interest. Methods of screening test agents that impact the protein-protein interaction are also provided.

Claim (Index 2):
A method of quantifying a protein-protein interaction between two cells in a sample, the method comprising:\n (a) providing a sample comprising a first cell expressing a first protein on its cell surface and a second cell expressing a second protein on its cell surface; (b) providing a first antibody binding composition specific for the first protein, wherein the first antibody binding composition comprises a molecular tag attached thereto via a cleavable linkage; (c) providing a second antibody binding composition specific for the second protein; (d) providing a third antibody composition that binds to the second antibody composition, the third antibody composition comprising a cleavage inducing moiety attached thereto; (e) contacting the sample with the first antibody binding composition, the second antibody binding composition, and the third antibody binding composition; (f) inducing cleavage of the molecular tag from the first antibody binding composition when the first antibody binding composition is within an effective proximity range of the cleavage inducing moiety attached to the third antibody binding composition, thereby releasing the molecular tag; and (g) quantitating the amount of released molecular tag as a measure of the amount of protein-protein interaction between the first protein and the second protein.

Metadata:
- Claim Count in Document: 40.0
- Percentile: 96.0
- Lexical Diversity: 1.58209
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12688798', '15663136', '15767801', '14802170', '15588271']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5584689553902595
- 35 USC 102 Novelty (BERT): 0.5171063404057491
- Combined Prediction Score: 0.5543326938918085
- Mean Citation Score: 283.6257260000001
- Max Citation Score: 293.78473
- Similarity Product: 209.33542924792468

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test