PATENT CLAIM ANALYSIS

Application Number: 16364947
Application Type: Utility
Filing Date: 2019-03
Publication Date: 2019-07
Patent Classification: ["435", "006120"]

Abstract:
Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.

Claim (Index 25):
A method, comprising:\n (a) tagging a plurality of nucleic acid molecules that have different nucleic acid sequences in a sample with a number of different barcodes comprising barcode sequences to generate a plurality of tagged nucleic acid molecules, wherein the tagged nucleic acid molecules comprise a barcode associated with the nucleic acid sequence of the nucleic acid molecule, wherein the barcode comprises at least two barcode sequences and wherein the barcode sequences are present in an asymmetric sequencing adapter prior to use for the tagging; (b) sequencing the plurality of tagged nucleic acid molecules or derivatives thereof to generate sequence reads, wherein the sequence reads comprise the sequence of the nucleic acid molecule and the sequence of the associated barcodes; (c) aligning the sequence reads to a reference sequence by the starting position and ending position of the sequence of the nucleic acid molecule, wherein the aligning is performed by a computer implemented method, wherein the reference sequence has a sense strand and an anti-sense strand; (d) determining which strand of the reference sequence the sequence reads align to, and (e) counting the number of unique barcodes associated with the nucleic acid molecule reads that align by starting position and ending position to the same location on the reference sequence, wherein the counting is performed by a computer implemented method, wherein a barcode is not classified as unique if a read with the same barcode maps to the same starting position and ending position on the corresponding anti-sense strand of the reference sequence.

Metadata:
- Claim Count in Document: 77.0
- Percentile: 99.0
- Lexical Diversity: 1.0
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15730157', '14006971', '14947988', '13476911', '15405301']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7040666653632239
- 35 USC 102 Novelty (BERT): 0.657199327432694
- Combined Prediction Score: 0.699379931570171
- Mean Citation Score: 447.6243720000001
- Max Citation Score: 617.7084
- Similarity Product: 581.4429736822127

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test