PATENT CLAIM ANALYSIS

Application Number: 15864378
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-08
Patent Classification: ["530", "412000"]

Abstract:
The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.

Claim (Index 18):
The method of  claim 1 , wherein\n (a) the load buffer and/or wash buffer comprise a salt selected from the group consisting of a sulfate salt, a citrate salt, ammonium sulfate, sodium sulfate, sodium chloride, ammonium chloride, sodium bromide and a combination thereof; (b) the load buffer and/or wash buffer comprise a cation selected from the group consisting of Ba2+, Ca2+, Mg2+, Li+, Cs+, Na+, K+, Rb+, NH4+ and a combination thereof; (c) the load buffer and/or wash buffer comprise an anion selected from the group consisting of PO43\u2212, SO42\u2212, CH3CO3\u2212, Cl\u2212, Br\u2212, NO3\u2212, ClO4\u2212, I\u2212, SCN\u2212 and a combination thereof; (d) the load buffer and/or wash buffer comprise a salt having a concentration of between about 50 mM and 2000 mM; (e) the load buffer and/or wash buffer have a pH between about 4.0 and 8.5 or between about 5.0 and 7.0; (f) the load buffer and/or wash buffer have a pH of about 4.0, about 4.5, about 5.0, about 5.5, about 6, about 6.5, about 7.0, about 7.5, about 8.0, or about 8.5; (g) the load buffer and/or wash buffer are the same; (h) the load buffer and/or wash buffer are substantially the same; and/or (i) the salt concentration and/or the pH of the wash buffer are within about 20%, 15%, 10% or 5% of the salt concentration and/or pH of the loading buffer.

Metadata:
- Claim Count in Document: 56.0
- Percentile: 86.0
- Lexical Diversity: 1.91935
- Patent Class: 530.0
- Transitional Phrase Type: closed
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14077574', '13831181', '14584492', '15545271', '14584619']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7252842667505169
- 35 USC 102 Novelty (BERT): 0.6380386947982708
- Combined Prediction Score: 0.7165597095552924
- Mean Citation Score: 392.83938
- Max Citation Score: 616.52576
- Similarity Product: 473.9093897826768

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test