PATENT CLAIM ANALYSIS

Application Number: 15900061
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2019-08
Patent Classification: ["435", "006100"]

Abstract:
The subject invention provides methods, assays, and products for detecting small molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target, preferably, a synthetic cathinone in a sample comprises contacting the sample with an aptamer-based sensor selective for the small-molecule target, and detecting the small-molecule target in the sample. Specifically, the method utilizes an aptamer-based sensor comprising a dye binding to a three-way junction binding domain of an aptamer. Binding of small-molecule target to the aptamer displaces the dye, generating a spectroscopic signal that can be used for detection of the small-molecule target and quantitative measurement of the target concentration.

Claim (Index 1):
An aptamer-based sensor comprising an aptamer having three fully complementary stems, and a three-way junction (TWJ) binding domain that binds Cy7 and a small-molecule target, at least one of the stem comprising a sequence selected from SEQ ID NO: 6 and SEQ ID NO: 7, the aptamer comprising at least 30 nucleotides and the small-molecule target being a synthetic cathinone having a core structure of: R 1  and R 2 , are each independently selected from the group consisting of hydrogen, alkyl, and alkoxy; or R 1  and R 2 , taken together with the carbon atoms to which they are attached, form a substituted or unsubstituted 5- or 6-membered methylenedioxy group or aromatic ring; R 3  is alkyl; R 4 , and R 5  are each independently selected from the group consisting of hydrogen and alkyl; or R 4  and R 5 , taken together with the nitrogen atom to which they are attached, form a substituted or unsubstituted 5- or 6-membered pyrrolidino group; and R 6  is hydrogen.

Metadata:
- Claim Count in Document: 38.0
- Percentile: 88.0
- Lexical Diversity: 2.03333
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14930242', '13092209', '11645362', '15878021', '13651265']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.54402410208197
- 35 USC 102 Novelty (BERT): 0.5101322808358368
- Combined Prediction Score: 0.5406349199573568
- Mean Citation Score: 249.76982
- Max Citation Score: 270.09644
- Similarity Product: 190.86738934319493

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test