PATENT CLAIM ANALYSIS

Application Number: 15921442
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-09
Patent Classification: ["435", "123000"]

Abstract:
Methods of producing peptide beta-lactones and beta-hydroxy acids are disclosed that include contacting a beta-hydroxy-alpha-amino acid, an aryl carrier protein (ObiD), and ATP with a non-ribosomal protein synthetase. A continuous flow reactor is disclosed that includes an elongate conduit with at least one region that includes a first region with a non-ribosomal protein synthetase immobilized to a substrate. The non-ribosomal protein synthetase of the continuous flow reactor is configured to contact a flow of a reaction mixture that includes a beta-hydroxy-alpha-amino acid and an aryl carrier protein. The non-ribosomal protein synthetase is further configured to release a peptide beta-lactone into the flow of the reaction mixture.

Claim (Index 7):
A method of producing a peptide beta-lactone, the method comprising:\n forming a reaction mixture comprising:\n a beta-hydroxy-alpha-amino acid selected from the group consisting of beta-OH-p-NO 2 -homoPhe and beta-OH-homoPhe; \n a benzoic acid derivative consisting of 2,3-dihydroxoybenzoic acid; \n ATP; \n an aryl carrier protein selected from the group consisting of ObiD, a homolog of ObiD, recombinant ObiD, and any variation thereof comprising the amino acid sequence of SEQ ID NO: 1 or fragment thereof; and \n a non-ribosomal protein synthetase selected from the group consisting of ObiF, a homolog of ObiF, recombinant ObiF, and any variation thereof comprising the amino acid sequence of SEQ ID NO:2 or fragment thereof, the non-ribosomal protein synthetase further comprising a condensation domain (C), a first adenylation domain (A 1 ), a peptidyl carrier domain (PCP), a thioesterase domain (TE), and a second adenylation domain (A 2 ); \n contacting the beta-hydroxy-alpha-amino acid with the non-ribosomal protein synthetase at the peptidyl carrier domain (PCP) with ATP activation by the first adenylation domain (A 1 ) to form a PCP-beta-hydroxy-alpha-amino acid thioester comprising an alpha-amino moiety; ATP-activating the benzoic acid derivative at the second adenylation domain (A 2 ) and contacting the ATP-activated benzoic acid derivative with the aryl carrier protein (ObiD) to form a benzoic acid derivative-aryl carrier protein (ObiD) thioester comprising a carbonyl moiety; contacting the benzoic acid derivative-aryl carrier protein (ObiD) thioester with the condensation domain (C) to catalyze an amide bond between the alpha-amino moiety and the carbonyl moiety to form a PCP-peptide beta-lactone precursor thioester; contacting the PCP-peptide beta-lactone precursor thioester with the thioesterase domain (TE) to form a transthioesterified peptide beta-lactone precursor; and releasing the transthioesterified peptide beta-lactone precursor as a peptide beta-lactone from the non-ribosomal protein synthetase (ObiF).

Metadata:
- Claim Count in Document: 11.0
- Percentile: 90.0
- Lexical Diversity: 2.07273
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12842549', '12118362', '10925357', '11775216', '10017324']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5325460610448187
- 35 USC 102 Novelty (BERT): 0.5067814365972625
- Combined Prediction Score: 0.5299695986000631
- Mean Citation Score: 208.44705400000004
- Max Citation Score: 260.4539
- Similarity Product: 187.55789502455

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test