PATENT CLAIM ANALYSIS

Application Number: 16038389
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2018-11
Patent Classification: ["435", "006100"]

Abstract:
Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

Claim (Index 54):
A stained biological sample, comprising:\n a first target comprising a polypeptide, a nucleic acid, or a tyrosine-containing moiety; a first binding complex comprising said first target, a first enzyme conjugate bound to said first target, and a first detection probe bound to said first enzyme conjugate, wherein the first enzyme conjugate comprises an enzyme selected from an oxidoreductase or a peroxidase; and a first signaling conjugate comprising a phenolic moiety and a first chromogenic moiety, wherein the phenolic moiety of said first signaling conjugate is covalently bound to:\n (i) a location on the first binding complex proximate to or directly on the first enzyme conjugate; \n (ii) a location on the first binding complex proximate to or directly on the first target; or \n (iii) a location on the biological sample proximate to the first target; \n wherein said stained biological sample is immobilized on a solid support, and wherein said stained biological sample, when analyzed by bright field microscopy, produces a signal that provides for the detection of said first target.

Metadata:
- Claim Count in Document: 56.0
- Percentile: 95.0
- Lexical Diversity: 1.96296
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13849160', '14769892', '14691826', '15246430', '15603079']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5862736799209096
- 35 USC 102 Novelty (BERT): 0.6601241403780357
- Combined Prediction Score: 0.5936587259666223
- Mean Citation Score: 419.83629200000007
- Max Citation Score: 733.6904
- Similarity Product: 594.2835844043733

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test