PATENT CLAIM ANALYSIS

Application Number: 16255718
Application Type: Utility
Filing Date: 2019-01
Publication Date: 2019-05
Patent Classification: ["435", "006120"]

Abstract:
Provided herein are products and processes for detecting the presence or absence of minor nucleic acid species in a sample containing a mixture of minor nucleic acid species and one or more major nucleic acid species, where the amount (frequency or copy number) of the minor nucleic acid species is less than that of the major nucleic acid species. Certain methods include amplifying the mixture and extending the resulting amplicons using chain terminating reagents and extension primers that specifically hybridize to the amplicons, where a chain terminating reagent specific for the major nucleic acid species has a concentration that is less than a chain terminating reagent that is specific for a minor nucleic acid species. Skewing the concentrations of the chain terminating reagents in favor of high concentrations of the chain terminating reagents specific for the minor nucleic acid species relative to a chain terminating reagent specific for a major nucleic acid species improves the detection limit (sensitivity) of detecting minor nucleic acid species present at low frequency or copy number in the mixture. In addition, the signals generated from the extension product of the major nucleic acid species amplicon can serve as a positive control and permit quantification of the minor nucleic acid species relative to the major nucleic acid species in the mixture.

Claim (Index 4):
The method of  claim 1 , wherein the sequence of the minor nucleic acid species comprises an insertion or a deletion relative to the sequence of the major nucleic acid species.

Metadata:
- Claim Count in Document: 44.0
- Percentile: 99.0
- Lexical Diversity: 2.69048
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15136024', '13551486', '14720179', '13193390', '10903268']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5384651816165887
- 35 USC 102 Novelty (BERT): 0.6147286903711892
- Combined Prediction Score: 0.5460915324920488
- Mean Citation Score: 304.66044
- Max Citation Score: 523.7029
- Similarity Product: 520.9983278966963

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test