PATENT CLAIM ANALYSIS

Application Number: 16431429
Application Type: Utility
Filing Date: 2019-06
Publication Date: 2019-10
Patent Classification: ["506", "008000"]

Abstract:
A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an α-helix structure, and is represented by the formula 2, wherein, in the formula 2, A 1  to A 12  independently represent an amino acid; X does not exist, or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an α-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3, wherein, in the formula 3, the amino acids are numbered “i” (−1), “ii” (−2), and so on from the C-terminus side, provided that L iii  to L vii  may not exist), and combination of three amino acids A 1 , A 4  and L ii , or combination of two amino acids A 4 , and L ii  is a combination corresponding to a target RNA base or base sequence.

Claim (Index 9):
A method for controlling a function of RNA, comprising:\n preparing a complex comprising a protein region consisting of a protein linked to a functional region, the protein containing one or more of PPR motifs, each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1:\n (HelixA)-X-(HelixB)-L\u2003\u2003(Formula 1) \n wherein, in formula 1, Helix A is a moiety of 12-amino acid length capable of forming an \u03b1-helix structure, and is represented by the formula 2:\n A 1 -A 2 -A 3 -A 4 -A 5 -A 6 -A 7 -A 8 -A 9 -A 10 -A 11 -A 12 \u2003\u2003(Formula 2) \n wherein, in the formula 2, A 1  to A 12  independently represent an amino acid; wherein, in the formula 1, X is a moiety of 1- to 9-amino acid length and is optional; wherein, in the formula 1, Helix B is a moiety of 11- to 13-amino acid length capable of forming an \u03b1-helix structure; and wherein, in the formula 1, L is a moiety of 2- to 7-amino acid length represented by the formula 3;\n L vii -L vi -L v -L iv -L iii -L ii -L i \u2003\u2003(Formula 3) \n wherein, in the formula 3, L i  to L vii  independently represent an amino acid, and L iii  to L vii  are optional, and wherein a combination of three amino acids A 1 , A 4  and L ii , or a combination of two amino acids A 4  and L ii  corresponds to the target sequence; preparing a cell containing an RNA having a target sequence; and introducing the complex into the cell, whereby the protein region of the complex binds to the RNA having the target sequence and the functional region modifies the function of the RNA.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 100.0
- Lexical Diversity: 2.5
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15335243', '15962127', '14352697', '14785952', '13989676']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7209290589981219
- 35 USC 102 Novelty (BERT): 0.6678549376307871
- Combined Prediction Score: 0.7156216468613884
- Mean Citation Score: 608.2416840000002
- Max Citation Score: 774.98267
- Similarity Product: 627.7743043781072

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test