PATENT CLAIM ANALYSIS

Application Number: 15750824
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-10
Patent Classification: ["506", "002000"]

Abstract:
Reagents and methods for the analysis of nucleic acids (e.g. genomic DNA) of circulating microparticles (i.e. microparticles originating from blood) are provided. The methods comprise linking at least two fragments of a target nucleic acid of a circulating microparticle to produce a set of at least two linked fragments of the target nucleic acid. In the methods, fragments of a target nucleic acid may be linked by techniques such as barcoding, partitioning, ligation and/or separate sequencing. The sequencing of a set of linked fragments provides a set of informatically linked sequence reads corresponding to the sequences of fragments from a single microparticle.

Claim (Index 56):
The method of  claim 55 , wherein the method comprises analysing a sample comprising at least two circulating microparticles, wherein each circulating microparticle contains at least two fragments of genomic DNA, and wherein the method comprises the steps of:\n (a) preparing the sample for sequencing comprising:\n (i) contacting the sample with a library of multimeric barcoding reagents comprising a multimeric barcoding reagent for each of the two or more circulating microparticles, wherein each multimeric barcoding reagent is as defined in  claim 55 ; and \n (ii) appending barcode sequences to each of the at least two fragments of genomic DNA of each circulating microparticle, wherein at least two barcoded target nucleic acid molecules are produced from each of the at least two circulating microparticles, and wherein the at least two barcoded target nucleic acid molecules produced from a single circulating microparticle each comprise the nucleic acid sequence of a barcode region from the same multimeric barcoding reagent; and \n (b) sequencing each of the barcoded target nucleic acid molecules to produce at least two linked sequence reads for each circulating microparticle.

Metadata:
- Claim Count in Document: 44.0
- Percentile: 88.0
- Lexical Diversity: 1.94915
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15842687', '14990276', '14006971', '14316447', '14947988']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5345642048664292
- 35 USC 102 Novelty (BERT): 0.536749168959476
- Combined Prediction Score: 0.5347827012757339
- Mean Citation Score: 344.23017
- Max Citation Score: 360.96902
- Similarity Product: 250.5937872408223

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test