PATENT CLAIM ANALYSIS

Application Number: 16005502
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["435", "006100"]

Abstract:
The disclosure provides methods and kits for preparing sequencing library to detect chromosomal abnormality using cell-free DNA (cfDNA) without the need of first isolating the cfDNA from a liquid fraction of a test sample. In some embodiments, the method involves reducing the binding between the cfDNA and nucleosomal proteins without unwinding the cfDNA from the nucleosomal proteins. In some embodiments, the reduction of binding may be achieved by treating with a detergent or heating. In some embodiments, the method further involves freezing and thawing the test sample before reducing the binding between the cfDNA and the nucleosomal proteins. In some embodiments, the test sample is a peripheral blood sample from a pregnant woman including cfDNA of both a mother and a fetus. In other embodiments, the test sample is a peripheral blood sample from a patient known or suspected to have cancer.

Claim (Index 1):
A method for obtaining sequence information from a whole blood sample comprising cell-free DNA, said method comprising:\n (a) obtaining a plasma fraction of the whole blood sample, wherein the plasma fraction comprises the cell-free DNA; (b) exposing the plasma fraction to conditions that reduce the binding of the cell-free DNA to nucleosomal proteins, wherein the conditions comprise exposing the plasma fraction to polysorbate-20, and/or heating the plasma fraction to a temperature from about 55\u00b0 C. to about 75\u00b0 C.; (c) attaching sequencing adapters to ends of unpurified cell-free DNA fragments in the plasma fraction without first purifying the cell-free DNA from the plasma fraction, thereby preparing a sequencing library comprising library fragments having the sequencing adapters attached to either end of the unpurified cell-free DNA fragments; and (d) sequencing said sequencing library to obtain sequence information.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 94.0
- Lexical Diversity: 2.09459
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14214277', '14802873', '14802936', '12958353', '15072255']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5437746384295532
- 35 USC 102 Novelty (BERT): 0.6638544357199095
- Combined Prediction Score: 0.5557826181585889
- Mean Citation Score: 453.48631000000006
- Max Citation Score: 644.9162
- Similarity Product: 631.6309896906257

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test