PATENT CLAIM ANALYSIS

Application Number: 15971863
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2018-09
Patent Classification: ["435", "320100"]

Abstract:
The present disclosure provides engineered polynucleotide sequences that form scaffolds and nucleoprotein complexes comprising such engineered polynucleotide sequences that form scaffolds and nucleic acid binding proteins. Nucleic acid sequences encoding the engineered polynucleotide sequences that form scaffolds, as well as expression cassettes, vectors and cells comprising such polynucleotide sequences, are described. A variety of methods for making and using the engineered polynucleotide sequences that form scaffolds are also disclosed.

Claim (Index 21):
A method of bringing a donor polynucleotide into proximity of a double-strand break (DSB) in DNA in a cell, comprising:\n introducing the DSB in the DNA, contacting a nucleic acid/protein composition with the DNA, the nucleic acid/protein composition comprising\n a composition of engineered Class 2 Type II CRISPR-nucleic acid sequences forming a scaffold (\u201cNASC\u201d), the Class 2 Type II CRISPR-NASC composition comprising\n a first engineered nucleic acid component (\u201cNASC-PC1\u201d) comprising, in a 5\u2032 to 3\u2032 direction, \n a spacer element 1 comprising a nucleic acid target binding sequence 1, wherein a nucleic acid target sequence 1 is in proximity to the DSB, \n a repeat element 1 comprising a repeat nucleic acid sequence 1, and \n a nucleic acid binding protein binding element 1 comprising a double-stranded nucleic acid binding protein binding sequence 1, \n wherein the spacer element 1 is covalently connected with the repeat element 1, and the repeat element 1 is covalently connected with the nucleic acid binding protein binding element 1; and \n a second engineered nucleic acid component (\u201cNASC-PC2\u201d) comprising, in a 5\u2032 to 3\u2032 direction, \n a spacer element 2 comprising a nucleic acid target binding sequence 2, \n a repeat element 2 comprising a repeat nucleic acid sequence 2, and \n a nucleic acid binding protein binding element 2 comprising a double-stranded nucleic acid binding protein binding sequence 2, \n wherein the spacer element 2 is covalently connected with the repeat element 2, and the repeat element 2 is covalently connected with the nucleic acid binding protein binding element 2; \n \n wherein there is a connection between the repeat nucleic acid sequence 1 and the repeat nucleic acid sequence 2 through hydrogen-bonded base pairs, and the connection forms the Class 2 Type II CRISPR-NASC composition, \n a first catalytically inactive Class 2 Type II CRISPR-Cas9 protein associated with the Class 2 Type II CRISPR-NASC composition and a second catalytically inactive Class 2 Type II CRISPR-Cas9 protein associated with the Class 2 Type II CRISPR-NASC composition; and \n a donor polynucleotide comprising a nucleic acid target sequence 2, wherein there is a connection between the nucleic acid target sequence 2 and the nucleic acid target binding sequence 2 through hydrogen-bonded base pairs; \n wherein the contacting facilitates the binding of the nucleic acid/protein composition to the nucleic acid target sequence 1, and bringing the donor polynucleotide into proximity of the double-strand break (DSB).

Metadata:
- Claim Count in Document: 59.0
- Percentile: 93.0
- Lexical Diversity: 1.82927
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15368570', '15703992', '15460642', '15331676', '15675677']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7372306246405013
- 35 USC 102 Novelty (BERT): 0.6679880464553534
- Combined Prediction Score: 0.7303063668219865
- Mean Citation Score: 661.3639
- Max Citation Score: 802.66437
- Similarity Product: 637.5446992029547

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test