PATENT CLAIM ANALYSIS

Application Number: 15873699
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["800", "278000"]

Abstract:
Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR associated protein 9 (Cas9) offer an effective way of creating targeted mutagenesis in plants. To alleviate concerns related to genetically modified plants, the present disclosure provides a novel and efficient genome editing system that allows the regeneration of mutant plants without DNA stable integration. This DNA free system utilizes Cas9 mRNA, guide RNA and selectable marker RNA to infect plant protoplasts. After a short period of selection of the transfected cells, non-transgenic plants carrying expected mutations were regenerated. The system offers a way of creating desired mutants without transgenic elements.

Claim (Index 16):
The method of  claim 1  further comprising exposing the plurality of cells to a second RNA molecule and a third RNA molecule in addition to the first RNA molecule under conditions sufficient to promote transfection of the second RNA molecule and third RNA molecule, wherein the second RNA molecule encodes Cas9, wherein the third RNA molecule is guide RNA (gRNA), wherein the gRNA comprises a scaffold sequence for Cas9 binding and a target sequence, wherein the target sequence defines the genomic region of the plurality of cells to be edited, wherein the second RNA molecule and third RNA molecule are sufficient to edit a genomic region of one or more of the plurality of cells, and wherein the plurality of cells are plant cells.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 86.0
- Lexical Diversity: 1.41772
- Patent Class: 800.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14885140', '14523799', '14183486', '14258458', '14577997']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5542512122651521
- 35 USC 102 Novelty (BERT): 0.4938834501875717
- Combined Prediction Score: 0.5482144360573941
- Mean Citation Score: 263.98526400000003
- Max Citation Score: 285.56033
- Similarity Product: 231.1752083429921

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test