PATENT CLAIM ANALYSIS

Application Number: 16164348
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-04
Patent Classification: ["435", "006100"]

Abstract:
According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.

Claim (Index 16):
A multiplex assay technique comprising\n (a) combining in a mixture:\n at least two different targets, \n at least two probes each comprising a target-specific moiety and a reporter dye, wherein each target-specific moiety is specific for a different target, and \n a reference dye having a structure according to formula (I): \n wherein: each of R 1  to R 3  and R 6  to R 8  is independently \u2014H, halogen, \u2014CO 2 H, \u2014CO 2 R, \u2014SO 3 H, \u2014SO 3 R, \u2014CH 2 CO 2 H, \u2014CH 2 CO 2 R, \u2014CH 2 SO 3 H, \u2014CH 2 SO 3 R, \u2014CH 2 NH 2 , \u2014CH 2 NHR, \u2014NO 2 , C 1 -C 6  alkyl, substituted C 1 -C 6  alkyl, C 1 -C 6  alkoxy, and substituted C 1 -C 6  alkoxy, wherein R is C 1 -C 6  alkyl, substituted C 1 -C 6  alkyl, C 1 -C 6  alkoxy, and substituted C 1 -C 6  alkoxy; R 4  and R 5  taken separately are selected from C 1 -C 6  alkyl, and C 1 -C 6  substituted alkyl, or R 4  and R 5  taken together are selected from C 3 -C 7  cycloalkyl, C 4 -C 7  unsaturated cycloalkyl, C 3 -C 7  substituted cycloalkyl, or C 4 -C 7  substituted unsaturated cycloalkyl; (b) irradiating the mixture with a first excitation wave length; and (c) detecting the radiation emitted by the at least two reporter dyes, which can each be measured in the presence of the other reporter dyes and the reference dye; wherein the assay indicates the presence, absence, amount, and/or identity of the at least two different targets in the mixture.

Metadata:
- Claim Count in Document: 32.0
- Percentile: 97.0
- Lexical Diversity: 1.90244
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14071552', '15707157', '15066248', '13165575', '14229663']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6653279094905846
- 35 USC 102 Novelty (BERT): 0.6001930724389581
- Combined Prediction Score: 0.658814425785422
- Mean Citation Score: 430.513642
- Max Citation Score: 568.27734
- Similarity Product: 470.1533620727491

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test