PATENT CLAIM ANALYSIS

Application Number: 16122586
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-01
Patent Classification: ["435", "008000"]

Abstract:
In a method for measuring luminescence of a biological sample utilizing two different luminescence reagents, the sample is agitated to improve mixing with the second luminescence reagent, allowing for a shorter delay time between injection of the second reagent and measurement of the resulting luminescence activity. The improved mixing may also allow for a shorter measurement time, thereby improving throughput when assaying a large number of samples.

Claim (Index 1):
A sample analysis apparatus, comprising:\n a sample carrier configured for agitating a biological sample; a liquid dispensing system configured for dispensing selected reagents into contact with the sample; a luminescence detector configured for measuring luminescence light emitted from the sample; and a computing device configured for controlling the sample carrier, the liquid dispensing system and the liquid dispensing system to perform the following steps:\n dispensing a biological sample into a well, supported by a motorized sample carrier of a sample analyzing apparatus, the sample analyzing apparatus comprising a liquid dispensing system, the liquid dispensing system comprising a first injection needle, a second injection needle, a first reagent reservoir communicating with the first injection needle, and a second reagent reservoir communicating with the second injection needle; \n aligning the first injection needle with the well by moving the sample carrier, moving the first injection needle, or moving both the sample carrier and the first injection needle; \n dispensing a first reagent from the first injection needle into the well at a flow rate of 100 \u03bcL/sec or less by operating the liquid dispensing system, wherein the sample reacts with the first reagent to emit a first luminescent light; \n after a first delay period, measuring the first luminescent light at a luminescence detector over a first measurement period; \n dispensing a second reagent from the second injection needle into the well at a flow rate of 100 \u03bcL/sec or less by operating the liquid dispensing system while the well remains supported by the sample carrier, wherein the second reagent comprises a luminescent reagent effective for inducing emission of a second luminescent light, and a quenching reagent effective for suppressing emission of the first luminescent light, and wherein the sample reacts with the second reagent to emit the second luminescent light; \n after a second delay period, measuring the second luminescent light at the luminescence detector over a second measurement period; and \n agitating the well to enhance mixing of the second reagent with the sample by operating the sample carrier to agitate the well in an automated manner, wherein: \n the agitating is performed while the sample remains in the well and the well remains supported on the sample carrier; and \n the agitating is initiated at a time selected from the group consisting of: while dispensing the second reagent; during the second delay period; and while measuring the second luminescent light.

Metadata:
- Claim Count in Document: 22.0
- Percentile: 97.0
- Lexical Diversity: 1.53191
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14808593', '14566708', '13311164', '12716219', '10760829']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5730986412949697
- 35 USC 102 Novelty (BERT): 0.5377108675410236
- Combined Prediction Score: 0.5695598639195751
- Mean Citation Score: 215.61238
- Max Citation Score: 387.27225
- Similarity Product: 320.71864982315896

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test