PATENT CLAIM ANALYSIS

Application Number: 16110682
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-12
Patent Classification: ["435", "006110"]

Abstract:
The invention provides methods, devices, compositions and kits for diagnosing or predicting transplant status or outcome in a subject who has received a transplant.

Claim (Index 1):
A method of quantifying kidney transplant-derived circulating cell-free deoxyribonucleic acid in a human kidney transplant recipient, said method comprising:\n (a) providing a plasma sample from said human kidney transplant recipient, wherein said human kidney transplant recipient has received a kidney transplant from a kidney transplant donor, wherein said plasma sample from said human kidney transplant recipient comprises kidney transplant-derived circulating cell-free deoxyribonucleic acid and human kidney transplant recipient-derived circulating cell-free deoxyribonucleic acid; (b) extracting circulating cell-free deoxyribonucleic acid from said plasma sample from said human kidney transplant recipient, wherein said extracted circulating cell-free deoxyribonucleic acid from said plasma sample from said human kidney transplant recipient comprises said kidney transplant-derived circulating cell-free deoxyribonucleic acid and human kidney transplant recipient-derived circulating cell-free deoxyribonucleic acid; (c) performing a selective amplification of target deoxyribonucleic acid sequences, wherein said selective amplification of said target deoxyribonucleic acid sequences is of said extracted circulating cell-free deoxyribonucleic acid from said plasma sample from said human kidney transplant recipient, wherein said selective amplification of said target deoxyribonucleic acid sequences amplifies a plurality of genomic regions comprising at least 1,000 single nucleotide polymorphisms, wherein said at least 1,000 single nucleotide polymorphisms comprise homozygous single nucleotide polymorphisms, heterozygous single nucleotide polymorphisms, or both homozygous single nucleotide polymorphisms and heterozygous single nucleotide polymorphisms, and wherein said selective amplification of saki target deoxyribonucleic acid sequences is by polymerase chain reaction (PCR); (d) performing a high throughput sequencing reaction, wherein said high throughput sequencing reaction comprises performing a sequencing-by-synthesis reaction on said selectively-amplified target deoxyribonucleic acid sequences from said extracted circulating cell-free deoxyribonucleic acid from said plasma sample from said human kidney transplant recipient, wherein said sequencing-by-synthesis reaction has a sequencing error rate of less than 1.5%; (e) providing sequences from said high throughput sequencing reaction, wherein said provided sequences from said high throughput sequencing reaction comprise said at least 1,000 single nucleotide polymorphisms; and (f) quantifying an amount of said kidney transplant-derived circulating cell-free deoxyribonucleic acid in said plasma sample from said human kidney transplant recipient, wherein said quantifying said amount of said kidney transplant-derived circulating cell-free deoxyribonucleic acid in said plasma sample from said human kidney transplant recipient comprises using markers distinguishabie between said human kidney tra.nspiant recipient and said kidney transplant donor, wherein said markers distinguishable between said human kidney transplant recipient and said kidney transplant donor comprise single nucleotide polymorphisms identified in said provided sequences from said high throughput sequencing reaction.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 96.0
- Lexical Diversity: 1.17391
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['16110646', '14188455', '13508318', '14536300', '15319271']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6570835643920101
- 35 USC 102 Novelty (BERT): 0.5549860219509635
- Combined Prediction Score: 0.6468738101479055
- Mean Citation Score: 357.328528
- Max Citation Score: 421.71204000000006
- Similarity Product: 301.4266955543375

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test