PATENT CLAIM ANALYSIS

Application Number: 16110150
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2019-02
Patent Classification: ["506", "026000"]

Abstract:
The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing (FISSEQ) in a new process referred to herein as “expansion sequencing” (ExSEQ).

Claim (Index 1):
A method for in-situ sequencing of target nucleic acids present in a biological sample comprising the steps of:\n a) linking target nucleic acids present in the biological sample with a small molecule linker or a nucleic acid adaptor capable of linking to a target nucleic acid and to a swellable material; b) embedding the biological sample comprising the target nucleic acids and attached small molecule linker or nucleic acid adaptor in a swellable material wherein the small molecule linker or the nucleic acid adaptor is linked to the target nucleic acids present in the sample and to the swellable material, c) digesting proteins present in the biological sample; d) swelling the swellable material to form a first enlarged biological sample that is enlarged as compared to the biological sample; e) re-embedding the first enlarged sample in a non-swellable material; (f) modifying the target nucleic acids or the nucleic acid adaptor to form a nucleic acid adaptor useful for sequencing; and (g) sequencing the nucleic acids present in the first enlarged sample.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 96.0
- Lexical Diversity: 1.61364
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15098968', '15789419', '15229539', '15730157', '15969118']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5243917098932562
- 35 USC 102 Novelty (BERT): 0.5493136502979822
- Combined Prediction Score: 0.5268839039337289
- Mean Citation Score: 264.73913200000004
- Max Citation Score: 389.3616
- Similarity Product: 360.0394308998108

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test