PATENT CLAIM ANALYSIS

Application Number: 15864378
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-08
Patent Classification: ["530", "412000"]

Abstract:
The present invention is directed to methods for purifying a protein of interest, e.g., an antibody, from a sample comprising the protein of interest and at least one impurity, e.g., an aggregate, by employing a hydrophobic interaction chromatography (HIC) method that allows for binding of both the protein of interest and the at least one impurity under strong binding conditions. The present invention is based, at least in part, on the finding that both flow through and bind-elute techniques can be combined to achieve greater purification and recovery of a protein of interest, e.g., an antibody, under isocratic wash conditions and strong binding conditions.

Claim (Index 15):
The method of  claim 1 , wherein\n (a) the portion of the protein of interest that binds to the HIC media is at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80% or at least about 90% of the protein of interest in the sample; (b) the substantial portion of the protein of interest released from the HIC media upon washing with the wash buffer is at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90% or about 100% of the amount of protein of interest bound to the HIC media; (c) the accumulative yield of the protein of interest in the flow through fraction and/or wash fraction is at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, or about 100%; (d) the accumulative yield of the protein of interest in any one flow through fraction and/or wash fraction is at least about 4%, at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85, at least about 90%, at least about 95% or about 100%; (e) the substantial portion of the at least one impurity that binds to the HIC media is at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95% or about 100% of the at least one impurity in the sample; (f) the reduced level of the at least one impurity of the flow through fraction and/or wash fraction is at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95% or about 100% of the at least one impurity in the sample; (g) the accumulative aggregate reduction of the at least one impurity in any one flow through fraction and/or wash fraction is at least about 0.1%, at least about 0.2%, at least about 0.5%, at least about 1.0%, at least about 2.0%, at least about 3.0%, at least about 4.0%, at least about 5.0%, at least about 10.0%, or at least about 20.0%; (h) the accumulative aggregate reduction of the at least one impurity in the flow through fraction and/or wash fraction is at least about 0.1%, at least about 0.2%, at least about 0.5%, at least about 1.0%, at least about 2.0%, at least about 3.0%, at least about 4.0%, at least about 5.0%, at least about 10.0%, or at least about 20.0%; and/or (i) the level of the at least one impurity is reduced by at least 60%, at least 70%, at least 80%, at least 90%, or at least 95% of the at least one impurity in the sample.

Metadata:
- Claim Count in Document: 56.0
- Percentile: 86.0
- Lexical Diversity: 1.91935
- Patent Class: 530.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14077574', '13831181', '14584492', '15545271', '14584619']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6998457484573565
- 35 USC 102 Novelty (BERT): 0.6280275664110044
- Combined Prediction Score: 0.6926639302527213
- Mean Citation Score: 392.83938
- Max Citation Score: 616.52576
- Similarity Product: 429.7082920427131

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test