PATENT CLAIM ANALYSIS

Application Number: 16107685
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2019-06
Patent Classification: ["435", "006100"]

Abstract:
The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

Claim (Index 1):
A method for analyzing cellular occupancy of partitions, comprising:\n (a) contacting a plurality of cells with a plurality of labelling molecules comprising a plurality of cell nucleic acid barcode sequences to generate a plurality of labelled cells, wherein each of said plurality of labelled cells comprises a different cell nucleic acid barcode sequence; (b) generating a plurality of partitions comprising said plurality of labelled cells and a plurality of beads, wherein said plurality of beads comprise a plurality of partition nucleic acid barcode sequences coupled thereto, wherein each of said plurality of partitions comprises a different partition nucleic barcode sequence, and wherein at least a fraction of said plurality of partitions comprises more than one labelled cell of said plurality of labelled cells; (c) within said plurality of partitions, (i) using partition nucleic acid barcode sequences of said plurality of partition nucleic acid barcode sequences and cell nucleic acid barcode sequences of said plurality of cell nucleic acid barcode sequences to synthesize a plurality of barcoded nucleic acid products, and (ii) releasing or recovering said plurality of barcoded nucleic acid products or derivatives thereof from said plurality of partitions; and (d) identifying-sequencing said plurality of barcoded nucleic acid products or derivatives thereof to determine that at least two labelled cells of said plurality of labelled cells originate from a same partition using (i) said cell nucleic acid barcode sequences of said plurality of cell nucleic acid barcode sequences or complements thereof and (ii) said partition nucleic acid barcode sequences of said plurality of partition nucleic acid barcode sequences or complements thereof.

Metadata:
- Claim Count in Document: 72.0
- Percentile: 96.0
- Lexical Diversity: 1.5
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15842687', '15887947', '15875899', '16052431', '15933299']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6124512856167985
- 35 USC 102 Novelty (BERT): 0.5378301070027112
- Combined Prediction Score: 0.6049891677553898
- Mean Citation Score: 376.471042
- Max Citation Score: 394.69577
- Similarity Product: 331.20333730749724

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test