PATENT CLAIM ANALYSIS

Application Number: 16063639
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-05
Patent Classification: ["435", "114000"]

Abstract:
The invention discloses a method for increasing the yield of L-arginine by knocking out flavin reductases, and belongs to the technical field of amino acid production by microbial fermentation. Genes frd1 and frd2 for encoding hypothetic NADPH-dependent FMN reductase in  Corynebacterium crenatum  SDNN403 are over-expressed in  E. coli  BL21 and are purified to form target proteins Frd181 and Frd188, and functions of the target proteins are identified to obtain a result showing that the proteins Frd181 and Frd188 both are NAD(P)H-dependent flavin reductases producing H 2 O 2 . By taking a genome of the  Corynebacterium crenatum  SDNN403 as a template, frd1 and frd2 gene deletion fragments are obtained by overlap extension PCR; connecting pK18mobsacB to obtain knockout plasmids pK18mobsacB-Δfrd1 and pK18mobsacB-Δfrd2; carrying out electric shock to transform the  Corynebacterium crenatum  SDNN403; and carrying out secondary screening to obtain recombinant strains 403Δfrd1 and 403Δfrd2. Found by flask shaking fermentation, the yield of L-arginine is obviously increased by knocking out the genes frd1 and frd2.

Claim (Index 6):
The method of  claim 5 , comprising carrying out a fermentation culture at 28-32\u00b0 C.; wherein components of a medium of the fermentation culture comprises: 120 g\u00b7L \u22121  of glucose, 40 g\u00b7L \u22121  of corn steep liquor, 8*10 \u22125  g\u00b7L \u22121  of biotin, 5*10 \u22124  g\u00b7L \u22121  of histidine, 0.02 g\u00b7L \u22121  of manganese sulfate, 20 g\u00b7L \u22121  of ammonium sulfate, 0.5 g\u00b7L \u22121  of magnesium sulfate, 1.5 g\u00b7L \u22121  of monopotassium phosphate and 0.02 g\u00b7L \u22121  of ferrous sulfate.

Metadata:
- Claim Count in Document: 14.0
- Percentile: 94.0
- Lexical Diversity: 1.72816
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14891622', '15891564', '15307455', '15876478', '12993215']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5343801031330173
- 35 USC 102 Novelty (BERT): 0.5004463687426904
- Combined Prediction Score: 0.5309867296939846
- Mean Citation Score: 207.173384
- Max Citation Score: 230.70525
- Similarity Product: 161.91562489056588

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test