PATENT CLAIM ANALYSIS

Application Number: 16272507
Application Type: Utility
Filing Date: 2019-02
Publication Date: 2019-05
Patent Classification: ["435", "190000"]

Abstract:
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.

Claim (Index 2):
An engineered polynucleotide encoding an engineered polypeptide having ketoreductase activity, wherein said engineered polypeptide comprises a region with an amino acid sequence having at least 90% sequence identity to residues-90 to 211 of SEQ ID NO: 130, comprising a substitution at position X190, and further comprising one or more of the following features selected from:\n residue corresponding to X94 is asparagine, glycine, or serine; residue corresponding to X95 is leucine or methionine; residue corresponding to X96 is glutamine, asparagine, or threonine; residue corresponding to X101 is glycine or asparagine; residue corresponding to X105 is glycine; residue corresponding to X108 is histidine or serine; residue corresponding to X112 is aspartic acid; residue corresponding to X113 is alanine; residue corresponding to X117 is serine; residue corresponding to X127 is arginine; residue corresponding to X147 is leucine; residue corresponding to X152 is methionine or lysine; residue corresponding to X157 is threonine; residue corresponding to X163 is isoleucine; residue corresponding to X176 is valine; residue corresponding to X194 is arginine or glutamine; residue corresponding to X197 is valine or glutamic acid; residue corresponding to X198 is glycine, glutamic acid, or lysine; residue corresponding to X199 is aspartic acid; residue corresponding to X200 is proline; residue corresponding to X202 is glycine; residue corresponding to X206 is a glycine; residue corresponding to X211 is a arginine or lysine; and wherein the amino acid sequence can optionally have one or more differences at other amino acid residues in the domain as compared to the reference sequence.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 99.0
- Lexical Diversity: 1.83333
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15711070', '16031177', '15211505', '13590882', '12545034']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.8033091518897926
- 35 USC 102 Novelty (BERT): 0.5975470453801878
- Combined Prediction Score: 0.7827329412388321
- Mean Citation Score: 515.2589019999998
- Max Citation Score: 519.2958
- Similarity Product: 509.9502673749446

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test