PATENT CLAIM ANALYSIS

Application Number: 15977875
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2019-02
Patent Classification: ["435", "006100"]

Abstract:
Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

Claim (Index 25):
A method of single-cell nucleic acid sequencing, the method comprising:\n (a) providing a device comprising: (i) a plurality of particles in an aqueous carrier, the particles comprising a nucleic acid primer sequence and a barcode; (ii) an aqueous sample comprising a population of cells; (iii) a particle channel; (iv) a sample channel; (v) a droplet formation region configured to allow the aqueous carrier to expand in at least one dimension; and (vi) a droplet collection region, wherein the particle channel intersects the sample channel proximal to the droplet formation region and the droplet formation region is proximal to the droplet collection region; (b) allowing the particles in the aqueous carrier to flow proximal-to-distal through the particle channel; (c) allowing the aqueous sample to flow proximal-to-distal through the sample channel, wherein the aqueous sample combines with the particles in the liquid carrier to form a reaction liquid at the intersection, and wherein the reaction liquid meets a partitioning liquid at the droplet formation region under droplet forming conditions, thereby forming a plurality of reaction droplets comprising one or more of the particles and a single cell or lysate thereof, wherein the plurality of droplets accumulate in the droplet collection region; (d) incubating the reaction droplets under conditions sufficient to generate barcoded nucleic acids; and (e) sequencing the barcoded nucleic acid transcripts to obtain nucleic acid sequences associated with single cells.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 93.0
- Lexical Diversity: 1.25806
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15977861', '15977805', '15977824', '09953103', '13249212']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6494980856103472
- 35 USC 102 Novelty (BERT): 0.5308864394154353
- Combined Prediction Score: 0.637636920990856
- Mean Citation Score: 312.415858
- Max Citation Score: 380.38614
- Similarity Product: 284.2700327223552

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test