PATENT CLAIM ANALYSIS

Application Number: 15746596
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2019-03
Patent Classification: ["514", "001100"]

Abstract:
The invention discloses a  Pediococcus acidilactici  derived antimicrobial hexapeptide and a preparation method thereof and belongs to the field of bioengineering. The antimicrobial peptide ENGEEE is reported for the first time. Antimicrobial peptide lactein R16 has good pH stability and thermal stability, has an effect on inhibiting  Escherichia coli, Listeria monocytogenes  and  Staphylococcus aureus  and is capable of effectively reducing the amount of the  Escherichia coli  in soybean meal. Secondly, the antimicrobial peptide lactein R16 has a certain effect on proliferating  Saccharomyces cerevisiae  and  Lactobacillus plantarum  and has certain capability of clearing hydrogen peroxide, hydroxyl radicals, DPPH.radicals and superoxide anions. The antimicrobial peptide lactein R16 can be used for biological control and can be used as a feed additive to play important roles in substituting antibiotics and solving the feed safety problem.

Claim (Index 10):
A preparation method of the antimicrobial peptide of  claim 1 , comprising the steps of: carrying out crude extraction by ultrafiltration, on a fermented supernatant obtained by fermenting  Pediococcus acidilactici ; then, carrying out separation by using an anion exchange chromatographic column Hitrap Q FF and a gel filtration chromatography Superdux Peptide 10/300GL: and finally, carrying out separation and purification by using a reversed-phase column Phenomenex Luna/C18 to obtain an antimicrobial hexapeptide.

Metadata:
- Claim Count in Document: 5.0
- Percentile: 86.0
- Lexical Diversity: 1.67059
- Patent Class: 514.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['12443609', '13224776', '14642158', '09642744', '13094155']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.728660520184924
- 35 USC 102 Novelty (BERT): 0.4927032607836065
- Combined Prediction Score: 0.7050647942447923
- Mean Citation Score: 166.683506
- Max Citation Score: 187.61557
- Similarity Product: 122.71728687030136

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test