PATENT CLAIM ANALYSIS

Application Number: 16158181
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-03
Patent Classification: ["435", "006100"]

Abstract:
Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to simultaneously detect both plant DNA and pathogen DNA in a single assay.

Claim (Index 26):
The method of  claim 18 , wherein the pathogen is a fungus, said second amplification forward and reverse primer pair having sequences shown respectively in SEQ ID NOS: 31 and 32, or SEQ ID NOS: 33 and 34, or SEQ ID NOS: 139 and 140.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 97.0
- Lexical Diversity: 1.71233
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['16158276', '15916036', '12100519', '09865807', '14694008']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.68146798919996
- 35 USC 102 Novelty (BERT): 0.6210317096937307
- Combined Prediction Score: 0.6754243612493371
- Mean Citation Score: 492.178766
- Max Citation Score: 703.1072
- Similarity Product: 482.3588561367035

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test