PATENT CLAIM ANALYSIS

Application Number: 16170208
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-02
Patent Classification: ["506", "009000"]

Abstract:
A method of using an exchange-induced remnant magnetization (EXIRM) technique for label free detection of short strands of nucleotides and cancer biomarkers, such as DNA and microRNA strands, DNA/RNA-binding biomarkers, and cancer-specific antigens, with high sensitivity, high specificity, and broad dynamic range. The method may provide a label-free approach aimed to facilitate high reliability, and to require a minimum amount of biochemical reagents.

Claim (Index 14):
An exchange induced remnant magnetization method to detect cancer biomarkers, the method comprising:\n (a) immobilizing a first sequence on a surface; wherein said first sequence comprises N bases; (b) adding a second sequence to said first sequence, wherein said second sequence comprises less than N complementary bases and a magnetic particle; and wherein said second sequence hybridizes to said first sequence forming a hybridized double strand; and (c) measuring a first magnetic signal value for said hybridized double strand; (d) incubating said hybridized double strand with a biomarker nucleic acid; and wherein said biomarker exchanges with said second strand to form an exchange product; wherein said exchange product is thermodynamically more stable than said hybridized double strand; (e) measuring a second magnetic signal value for the exchange product of step (c) after applying a weak mechanical force to remove nonspecifically bound magnetic particles; and (f) quantifying the amount of said biomarker from the difference in magnetic signal values measured in step (c) and step (e) wherein the difference in the magnetic signal values comprises a decrease in magnetic signal values due to the randomization of the second sequence magnetic dipoles.

Metadata:
- Claim Count in Document: 32.0
- Percentile: 97.0
- Lexical Diversity: 1.39623
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12835869', '11958180', '15450675', '15209090', '14571142']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.622470497516118
- 35 USC 102 Novelty (BERT): 0.5148558256674947
- Combined Prediction Score: 0.6117090303312558
- Mean Citation Score: 276.811358
- Max Citation Score: 279.44
- Similarity Product: 169.61731325149535

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test