PATENT CLAIM ANALYSIS

Application Number: 16146807
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-01
Patent Classification: ["435", "006100"]

Abstract:
Provided herein is a method of sequencing hydroxymethyated cell-free DNA. In some embodiments, the method comprises adding an affinity tag to only hydroxymethyated DNA molecules in a sample of cfDNA, enriching for the DNA molecules that are tagged with the affinity tag; and sequencing the enriched DNA molecules.

Claim (Index 2):
A method of sample analysis, comprising:\n (a) identifying hydroxymethylated sequences in a cell-free DNA (cfDNA) sample from a patient by: (i) ligating adaptor sequences to the cfDNA, (ii) modifying 5-hydroxymethylcytosine (5hmC) residues in the cfDNA to contain a capture tag; (iii) enriching for cfDNA comprising hydroxymethylcytosine residues by linking the cfDNA to a support via the capture tag, thereby providing an enriched composition comprising support-bound hydroxymethylated cfDNA, (iv) amplifying the cfDNA molecules without releasing the hydroxymethylated cfDNA from the support, to provide amplified DNA, and (v) sequencing the amplified DNA to produce a plurality of sequence reads, and (vi) identifying hydroxymethylated sequences in the sequence reads; (b) comparing the identified hydroxymethylated sequences with a set of signature hydroxymethylation sequences that are correlated with a phenotype; and (c) providing a report indicating a correlation with the phenotype.

Metadata:
- Claim Count in Document: 54.0
- Percentile: 97.0
- Lexical Diversity: 1.47222
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14983379', '13555037', '13009718', '12958353', '13600043']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5470478667150611
- 35 USC 102 Novelty (BERT): 0.5141146355991485
- Combined Prediction Score: 0.5437545436034699
- Mean Citation Score: 238.7877
- Max Citation Score: 248.1314
- Similarity Product: 133.11766895115375

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test