PATENT CLAIM ANALYSIS

Application Number: 16191369
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-03
Patent Classification: ["524", "555000"]

Abstract:
Micro-nano materials, products obtained by covalently modifying the surfaces of micro/nano materials with hydrophilic materials, and methods for making the same. The micro/nano materials on the surfaces have carboxyl groups or/and pro-carboxyl groups which are converted into their active esters. The products are covalently modified by forming amide bonds between the active esters on the surfaces and the modification agents; where the modification agents are hydrophilic compounds and/or hydrophilic polymers bearing primary and/or secondary aliphatic amines. Monomers bearing carboxyl groups and/or pro-carboxyl groups are used to produce an adequate number of carboxyl groups and/or pro-carboxyl groups on the surface of a polymer material to be modified. The carboxyl groups and/or pro-carboxyl groups are converted into active esters. A reasonably-sized modification agent bearing primary and/or secondary amines, zwitterions and hydrophilic linear spacer arms is used to form amide bonds and obtain a covalently modified surface layer.

Claim (Index 8):
The method of  claim 5 , wherein in step c, the protein-disulfide-selective modification group has the following characteristics:\n the protein-disulfide-selective modification group comprises two reactive functional groups enabling Michael addition and/or nucleophilic substitution reaction with a sulfhydryl group, with no more than 10 covalent bonds located between the two reactive functional groups, besides a carboxyl group for forming an amide bond with other components; the reactive group undergoing Michael addition reaction with sulfhydryl group is an acryloyl group, or a vinyl sulfone group, and when functioning alone, acts as a pro-carboxyl group; its reaction center is \u03b2 position of carbonyl in acroleyl group and \u03b2 olefin carbon atom of sulfonyl group; the reactive group undergoing nucleophilic substitution reaction with a sulfhydryl group is an alkyl containing one or more of chlorine, bromine, iodine, trifluoroacetate, p-toluenesulfonate at \u03b1-saturated carbon atom of a carbonyl group, a sulfone group, an olefin, and an aromatic ring, and a group from which the alkyl is derived after the Michael-addition of the protein sulfhydryl group, and such a reactive group acting alone is a pro-carboxyl group; chlorine, bromine, iodine, trifluoroacetate, and p-toluenesulfonate are the leaving group X of the nucleophilic substitution reaction, and the reaction center of the nucleophilic substitution is an \u03b1-saturated carbon atom corresponding to the carbonyl group, the sulfone group, the olefin and the aromatic ring; wherein in the protein-disulfide-selective modification group, the fragment linking two reaction centers of sulfhydryl groups does not contain a ring or a trialkyl substituent, and a five- or six-membered ring as the covalent linking fragment is attached directly and simultaneously to two functional groups reactive with the protein sulfhydryl groups; the carboxyl group contained in the protein-disulfide-selective modification group for covalently linking with the micro/nano material, is converted into an active ester, an acid anhydride or an acid chloride to react with the primary and secondary amine on the surface of the micro/nano material, so as to obtain the protein disulfide selective modification functional group on the surface of the modified micro/nano material; when the protein-disulfide-selective modification group is used, the disulfide bond on the surface of the target protein is first reduced to two spatially adjacent free sulfhydryl groups by trialkylphosphine, and then such two spatially adjacent protein sulfhydryl groups simultaneously react with the two reaction centers in the protein-disulfide-selective modification group on the surface of the modified micro/nano material, thereby achieving site-selective covalent attachment/immobilization for the protein disulfide bond; and wherein the application of the protein-disulfide-selective modification group has the following characteristics: the carboxyl group contained in the protein disulfide selective modification functional group is covalently linked to the amine group of a polymer/small molecule having no sulfhydryl groups and no disulfide bonds on the surface, to obtain the protein-disulfide-selective modification group as a selective modification/labeling agent for disulfide bonds on protein surface; trialkylphosphine is used to reduce the disulfide bond on protein surface to two adjacent free sulfhydryl groups, to simultaneously react with the two reactive groups in the protein-disulfide-selective modification group in the selective modification/labeling agent, thereby achieving the site-specific covalent attachment or labeling.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 98.0
- Lexical Diversity: 2.09459
- Patent Class: 524.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['13751295', '14639035', '14114813', '15398288', '15398254']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.8172727062156334
- 35 USC 102 Novelty (BERT): 0.4785396250126972
- Combined Prediction Score: 0.7833993980953398
- Mean Citation Score: 181.042228
- Max Citation Score: 186.06807
- Similarity Product: 128.2099964093292

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test