PATENT CLAIM ANALYSIS

Application Number: 15874673
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["435", "040500"]

Abstract:
Described herein is a bone tissue clearing method with enhanced optical access. Compositions and techniques for bone tissue clearing include continuous convective flow during the clearing process, amino alcohol to minimize tissue autofluorescence, and an imaging procedure that minimizes refractive index variations in light-sheet microscopy. These improvements allowed the Inventors to achieve whole-bone clearing with an imaging depth of up to about 1.5 mm while maintaining fluorescence and a signal-to-noise ratio (SNR) that permits detection and 3D placement of single cells. In various embodiments, the present application teaches methods and kits for clearing and optionally subsequently visualizing tissue containing bone.

Claim (Index 20):
A method comprising:\n fixing a bone tissue sample by applying a fixative solution comprising 4% paraformaldehyde (PFA) to generate a fixed bone tissue sample; decalcifying a fixed bone tissue sample by applying a decalcifying solution comprising ethylenediaminetetraacetic acid (EDTA) and stirring to generate a decalcified bone tissue sample; stabilizing the decalcified bone tissue sample by applying a hydrogel monomer solution comprising 4% acrylamide with flow assistance; initiating polymerization of the monomer solution to generate a decalcified bone tissue sample hydrogel matrix; washing the decalcified bone tissue sample hydrogel matrix with flow assistance; removing lipids from the washed decalcified bone tissue sample hydrogel matrix by applying a detergent solution comprising 8% sodium dodecyl sulfate (SDS) with flow assistance to generate a substantially cleared bone tissue sample; removing heme from the substantially cleared bone tissue sample by applying a removal solution comprising 25% N,N,N\u2032,N\u2032-Tetrakis(2-Hydroxypropyl)ethylenediamine with flow assistance to generate a cleared bone tissue sample; and further washing the cleared bone tissue sample with constant stirring.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 86.0
- Lexical Diversity: 1.37975
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14447607', '14932819', '13174311', '14543234', '14403050']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5216203531666582
- 35 USC 102 Novelty (BERT): 0.5083267372438662
- Combined Prediction Score: 0.5202909915743791
- Mean Citation Score: 205.693828
- Max Citation Score: 271.43472
- Similarity Product: 202.38916542800905

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test