PATENT CLAIM ANALYSIS

Application Number: 15954805
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "190000"]

Abstract:
It relates to a  Mortierella alpine  ATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention,  Mortierella alpine  ATCC32222 is used as a material and undergoes gene knockout through an  Agrobacterium tumefaciens  mediated genetic manipulation technology, to obtain the  Mortierella alpine  uracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungus  Mortierella alpine  ATCC32222 and product development.

Claim (Index 1):
A method of constructing a  Mortierella alpina  ATCC 32222 uracil auxotroph strain, which is generated by inactivating the ura5 encoding orotate phosphoribosyltransferase (OPRTase), in which the inactivation is achieved through the deletion of the 18 bp (from 213 bp to 230 bp) of the 654 bp ura5 genome DNA having a nuclei acid sequence shown as SEQ ID NO: 2, characterized in that it inactivates ura5 gene through the deletion of the 18 bp (from 213 bp to 230 bp) of the 654 bp in  Mortierella alpina  by homologous recombination, in which the homologous DNA sequences are the 1393 bp (from \u22121180 bp to +212 bp) up-stream and the 1362 bp (from +231 bp to +1592 bp) down-stream of the  M. alpina  ura5 genome DNA sequence having a nuclei acid sequence shown as SEQ ID NO: 3, the steps of the said method are as follows: acquisition of the up- and down-stream sequences of ura5 gene; construction of knockout plasmid pBIG4KOura5; transformation of  Agrobacterium tumefaciens  C58C1 with plasmid pBIG4KOura5; transformation of  M. alpina  with the  A. tumefaciens  C58C1 (harboring pBIG4KOura5) using the  Agrobacterium tumefaciens -mediate transformation (ATMT) method, then screening and identifying the uracil auxotroph to obtain the uracil auxotrophic stain of  M. alpine ; wherein the uracil auxotrophic stain of  M. alpine  is  Mortierella alpina  MAU1 deposited at the General Microbiology Culture Collection Center of China Committee for Culture Collection of Microorganisms under accession number CGMCC No. 8414.

Metadata:
- Claim Count in Document: 11.0
- Percentile: 91.0
- Lexical Diversity: 1.4898
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14910675', '16060417', '10454125', '12512930', '15675916']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6449281298417916
- 35 USC 102 Novelty (BERT): 0.5675374675490125
- Combined Prediction Score: 0.6371890636125137
- Mean Citation Score: 337.012824
- Max Citation Score: 508.22256
- Similarity Product: 348.15441681993485

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test