PATENT CLAIM ANALYSIS

Application Number: 15741456
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-08
Patent Classification: ["435", "034000"]

Abstract:
The present invention relates to a novel method for rapid assessment of one or more subclasses of blood cells of interest (BCol), as for example CD4+ cells and CD8+ cells, in a liquid whole blood sample or a sample derived therefrom; a method of determining the cell count for such cells; a method for determining the CD4/CD8 ratio; a method for determining the quantity of such receptors in a sample; as well as a vertical flow assay device for performing such assessment.

Claim (Index 28):
The method  claim 1 , comprising the steps of\n (1a) mixing the said sample or an aliquot of the said sample with a first liquid comprising antibodies binding to other structures on the surface of other cells different from said specific sub-group of cells but carrying said CD4 receptors, forming particles or aggregates or clusters of particles or cells with a size significantly larger than the size of the cells in said specific sub-group of cells, (1b) filter away said formed particles or aggregates or cluster of particles or cells by means of a first filter that is constituted by a size exclusion filter, and (2) passing the remaining mixture through a second filter retaining the said specific sub-group of cells (in said sample but letting CD4 receptor molecules in solution pass through the filter, optionally followed by a washing step, (3a) followed by exposing the said second filter to a liquid comprising labeled antibodies specifically reactive to said CD4 receptors, where said label is constituted by an enzyme or colored or fluorescent particle, optionally followed by a washing step, (3b) optionally followed by adding a substrate to said enzyme generating a colored or fluorescent substance, and (3c) measuring the intensity of the color or the fluorescence on said second filter and correlating said intensity to the concentration of said class of CD4 receptors on the surface of the said specific sub-group of cells

Metadata:
- Claim Count in Document: 83.0
- Percentile: 86.0
- Lexical Diversity: 1.78431
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['13863490', '12443306', '09888959', '12215501', '12293046']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5956940868065856
- 35 USC 102 Novelty (BERT): 0.4966885610267809
- Combined Prediction Score: 0.5857935342286053
- Mean Citation Score: 230.07016400000003
- Max Citation Score: 256.25378
- Similarity Product: 188.90366330858467

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test