PATENT CLAIM ANALYSIS

Application Number: 16017157
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["800", "023000"]

Abstract:
The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.

Claim (Index 7):
The method of  claim 1 , wherein the agent contacts any one or more of:\n (a) the mammalian oocyte prior to nuclear transfer; (b) the mammalian SCNT embryo prior to or at 5 hours post activation; (c) the mammalian SCNT embryo at the 1-cell stage; (d) the mammalian SCNT embryo after 5 hours post activation (5 hpa), after 12 hours post activation (12 hpa), or after 20 hours post activation (20 hpa); (e) the mammalian SCNT embryo between 5-28 hour post-activation (5-28 hpa), between 10-12 hours post activation (10-12 hpa), or between 20-28 hours post activation (20-28 hpa); (f) the mammalian SCNT embryo at the 2-cell stage; (g) the cytoplasm or nucleus of the donor mammalian cell prior to transfer of the nucleus into the enucleated mammalian oocyte; (h) the mammalian cell at least 24 hours, 48 hours, or 72 hours prior to transfer of the nucleus into the mammalian oocyte; or (i) the nucleus of the mammalian cell prior to removal of the nucleus from the mammalian cell for transfer into the mammalian oocyte.

Metadata:
- Claim Count in Document: 32.0
- Percentile: 94.0
- Lexical Diversity: 1.80597
- Patent Class: 800.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14276810', '12122557', '12528212', '14775594', '10865369']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.625907259729657
- 35 USC 102 Novelty (BERT): 0.546044277078621
- Combined Prediction Score: 0.6179209614645534
- Mean Citation Score: 354.94936399999995
- Max Citation Score: 431.14233
- Similarity Product: 286.61480706799864

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test