PATENT CLAIM ANALYSIS

Application Number: 15917549
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-11
Patent Classification: ["435", "006110"]

Abstract:
The embodiments disclosed herein utilized RNA targeting effectors to provide a robust CRISPR-based diagnostic with attomolar sensitivity. Embodiments disclosed herein can detect broth DNA and RNA with comparable levels of sensitivity and can differentiate targets from non-targets based on single base pair differences. Moreover, the embodiments disclosed herein can be prepared in freeze-dried format for convenient distribution and point-of-care (POC) applications. Such embodiments are useful in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, sensitive genotyping, and detection of disease-associated cell free DNA.

Claim (Index 8):
The system of  claim 7 , wherein the masking construct comprises:\n a. a silencing RNA that suppresses generation of a gene product encoded by a reporting construct, wherein the gene product generates the detectable positive signal when expressed; b. a ribozyme that generates the negative detectable signal, and wherein the positive detectable signal is generated when the ribozyme is deactivated; or c. a ribozyme that converts a substrate to a first color and wherein the substrate converts to a second color when the ribozyme is deactivated; d. an aptamer and/or comprises a polynucleotide-tethered inhibitor; e. a polynucleotide to which a detectable ligand and a masking component are attached; f. a nanoparticle held in aggregate by bridge molecules, wherein at least a portion of the bridge molecules comprises a polynucleotide, and wherein the solution undergoes a color shift when the nanoparticle is disbursed in solution; g. a quantum dot linked to one or more quencher molecules by a linking molecule, wherein at least a portion of the linking molecule comprises a polynucleotide; h. a polynucleotide in complex with an intercalating agent, wherein the intercalating agent changes absorbance upon cleavage of the polynucleotide; or i. two fluorophores tethered by a polynucleotide that undergo a shift in fluorescence when released from the polynucleotide.

Metadata:
- Claim Count in Document: 41.0
- Percentile: 90.0
- Lexical Diversity: 1.38889
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15917546', '15467922', '15916271', '14975085', '15897089']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6852526439313504
- 35 USC 102 Novelty (BERT): 0.6234178229107884
- Combined Prediction Score: 0.6790691618292943
- Mean Citation Score: 415.336298
- Max Citation Score: 717.7631
- Similarity Product: 498.65103345580695

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test