PATENT CLAIM ANALYSIS

Application Number: 16120019
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-12
Patent Classification: ["435", "006120"]

Abstract:
Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

Claim (Index 37):
A method of analyzing a plurality of double-stranded nucleic acid fragments comprising:\n a) attaching both ends of each of the plurality of double-stranded nucleic acid fragments to proximal ends of adapters selected from a pool of adapters, and wherein the pool of adapters comprises adapters having:\n a double-stranded portion at the proximal end, and \n a single-stranded portion at a distal end, wherein the single-stranded portion comprises a single-stranded tag substantially unique to the adapter; \n b) amplifying both strands of the adapter-nucleic acid products to produce first amplicons and second amplicons, wherein the first amplicons are derived from a first strand of the double-stranded nucleic acid fragments and the second amplicons are derived from a second strand of the double-stranded nucleic acid fragments, and wherein each of the first and second amplicons contain a tag; c) determining the sequence of the first and second amplicons; and d) in first and second amplicons originating from a single double-stranded nucleic acid fragment\u2014\n identifying sequence variations in the first and second amplicons, wherein the sequence variations from the first and second amplicons are consistent sequence variations; or \n eliminating sequence variations that occur in the first but not the second amplicons.

Metadata:
- Claim Count in Document: 89.0
- Percentile: 96.0
- Lexical Diversity: 1.51639
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15660785', '16120072', '16120091', '14386800', '12069174']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6950027495065458
- 35 USC 102 Novelty (BERT): 0.6654042727207545
- Combined Prediction Score: 0.6920429018279667
- Mean Citation Score: 630.1150099999999
- Max Citation Score: 727.7
- Similarity Product: 621.0753872632981

Labels:
- Claim Label 101: 0
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test