PATENT CLAIM ANALYSIS

Application Number: 15754005
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-09
Patent Classification: ["702", "025000"]

Abstract:
A method for determining fluorescence values {φ i spe } i∈{1, 2, . . . , I}  of a set of I fluorescent microparticles {μP i } i∈{1, 2, . . . , I}  of a multiplexed analysis, the microparticles being in a monolayer arrangement, includes acquiring a digital fluorescence image of the set of fluorescent microparticles {μP i } i∈{1, 2, . . . , I} ; and computing, for each fluorescent microparticle μP i  in the set of fluorescent microparticles {μP i } i∈{1, 2, . . . , I} , a fluorescence value φ i meas  based only on pixels of the acquired image corresponding to said fluorescent microparticle μP i . The method includes computing the fluorescence value φ i spe  of the fluorescent microparticle μP i  by correcting its first fluorescence φ i meas  by a cross-talk fluorescence contribution φ i cross  in the first fluorescence φ i meas  from other fluorescent microparticles {μP j } j≠i  in the set of fluorescent microparticles {μP i } i∈{1, 2, . . . , I} .

Claim (Index 26):
A system for determining fluorescences {\u03c6 i spe } i\u2208{1, 2, . . . , I}  of a set of I fluorescent encoded microparticles {\u03bcP i } i\u2208{1, 2, . . . , I}  being encoded in silicon micro-disks carrying a binary code comprising:\n at least one channel for receiving the set of I fluorescent encoded microparticles {\u03bcP i } i\u2208{1, 2, . . . , I}  in a monolayer arrangement; an acquisition unit for acquiring a digital fluorescent image of the monolayer arrangement of the set fluorescent encoded microparticles {\u03bcP i } i\u2208{1, 2, . . . , I}  in the channel; a computing unit for computing the fluorescences {\u03c6 i spe } i\u2208{1, 2, . . . , I}  based on the acquired digital fluorescent image, the computing unit computing a first fluorescence \u03c6 i meas  based only on pixels of the acquired digital fluorescent image corresponding to said fluorescent encoded microparticle \u03bcP i , said computing unit being configured to obtain the fluorescence value \u03c6 i spe  of a fluorescent encoded microparticle \u03bcP i  using each fluorescence values \u03c6 i meas ; wherein the computing unit computes, for each fluorescent encoded microparticle \u03bcP i  in the set of fluorescent encoded microparticles {\u03bcP i } i\u2208{1, 2, . . . , I} : a cross-talk fluorescence contribution \u03c6 i cross , wherein the cross-talk fluorescence contribution \u03c6 i cross  is modeled as a sum of individual contributions, each having an isotropic decay profile, based on the following relationship: \u03d5 i cross = \u2211 j \u2260 i \ue89e ( \u03b1 ij \u00b7 \u03d5 j spe ) where \u03b1 ij  is a unitary cross-talk fluorescence contribution in the first fluorescence \u03c6 i meas  of the j th  fluorescent microparticle \u03bcP i  depending only on the distance d i,j  between microparticles \u03bcP i  and \u03bcP j  and\n \u03c6 j spe  is the fluorescence of the j th  fluorescent encoded microparticle \u03bcP j ; and \n the fluorescence \u03c6 i spe  of said fluorescent encoded microparticle \u03bcP i  by correcting its first fluorescence \u03c6 i meas  by the cross-talk fluorescence contribution \u03c6 i cross .

Metadata:
- Claim Count in Document: 41.0
- Percentile: 88.0
- Lexical Diversity: 3.4918
- Patent Class: 702.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14452085', '13089961', '10415275', '11179135', '13212324']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.2002609463121824
- 35 USC 102 Novelty (BERT): 0.490484045338972
- Combined Prediction Score: 0.2292832562148614
- Mean Citation Score: 198.72079
- Max Citation Score: 230.30417000000003
- Similarity Product: 165.84392385386647

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test