PATENT CLAIM ANALYSIS

Application Number: 16060405
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-01
Patent Classification: ["424", "186100"]

Abstract:
A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

Claim (Index 1):
A method for separating AAV9 viral particles having packaged genomic sequences from genome-deficient AAV9 capsid intermediates, said method comprising:\n subjecting a mixture comprising recombinant AAV9 viral particles and AAV9 capsid intermediates to fast performance liquid chromatography, wherein the AAV9 viral particles and AAV9 intermediates are bound to an anion exchange resin equilibrated at a pH of about 10.2 and subjected to a salt gradient while monitoring eluate for ultraviolet absorbance at about 260 and about 280, wherein the AAV9 full capsids are collected from a fraction which is eluted when the ratio of A260/A280 reaches an inflection point.

Metadata:
- Claim Count in Document: 4.0
- Percentile: 94.0
- Lexical Diversity: 1.28846
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15081001', '13328306', '15544498', '10166347', '10016767']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7412583591489263
- 35 USC 102 Novelty (BERT): 0.5305159308203977
- Combined Prediction Score: 0.7201841163160735
- Mean Citation Score: 340.52553
- Max Citation Score: 366.07227
- Similarity Product: 286.6810603813112

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test