PATENT CLAIM ANALYSIS

Application Number: 15950982
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "006110"]

Abstract:
Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.

Claim (Index 38):
A microfluidic device configured to non-destructively obtaining a genotype of a plant cell, the device comprising:\n an inlet configured to receive a plant cell sample comprising a plurality of plant cells; a microfluidic channel in fluid communication with the inlet; an obstacle array configured to focus the plant cell sample to a sidewall of the microfluidic channel; an electrode array configured to separate a first constituent from the focused plant cell sample by deflecting the first constituent of the focused plant cell sample towards a central portion of the microfluidic channel; a single-cell trapping array located downstream from the electrode array, the single-cell trapping array comprising a microwell array configured to trap a single plant cell from the first constituent of the plant cell sample in a microwell of the microwell array; a polymer membrane encapsulating the single-cell trapping array; and an external micro-manipulating instrument configured to pierce the polymer membrane and penetrate a respective cell wall of the trapped single plant cell to extract mRNA from the trapped single plant cell using a dielectrophoresis (DEP) force under an alternating current (AC) field applied to the external micro-manipulating instrument.

Metadata:
- Claim Count in Document: 32.0
- Percentile: 91.0
- Lexical Diversity: 2.25758
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15288969', '15697898', '13625836', '14733539', '13464893']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5970741019999297
- 35 USC 102 Novelty (BERT): 0.6078571890998331
- Combined Prediction Score: 0.5981524107099201
- Mean Citation Score: 276.89023
- Max Citation Score: 556.32245
- Similarity Product: 487.95452756756544

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test