PATENT CLAIM ANALYSIS

Application Number: 15893502
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["315", "149000"]

Abstract:
Laboratory optical imaging systems for fluorescence and bioluminescence use a sensitive charge-coupled device (CCD) camera to produce quantitative measurements of very low light intensity, detecting signals from specimens labeled with optical fluorophores or luminescent emitters. Commercially available systems typically provide quantitative measurements of light output, in units of radiance (photons s −1  cm −2  SR −1 ) or intensity (photons s −1  cm −2 ). We describe a quality assurance system for low-light imagers, based on an LED-illuminated thin-film transistor (TFT) liquid crystal display module. The light intensity is controlled by pulse-width modulation of the backlight, producing intensity values ranging from 1×10 6  photons s −1  cm −2  to 4×10 13  photons s −1  cm −2 . The lowest light intensity values are produced by very short backlight pulses (i.e. approximately 10 μs), repeated every 300 s. This light source provides a stable, traceable intensity standard that can be used for routine quality assurance of optical imaging systems.

Claim (Index 1):
A method for calibrating optical intensity levels, spatial resolution and geometric scaling factors for a low-light-level imaging system, the method comprising:\n a. providing a thin-film transistor liquid crystal display module with a light-emitting diode backlight within a low-light level imaging system, to serve as a calibration phantom; b. modulating the average light intensity of the thin-film transistor liquid crystal display module by pulse-width modulation of the light-emitting diode backlight; c. calibrating the average light intensity of the thin-film transistor liquid crystal display module in absolute light intensity units; d. calibrating the geometric spacing of the pixels within the thin-film transistor liquid crystal display module in absolute distance units; e. obtaining a 2D image of the calibration phantom using a high-sensitivity, cooled charge-coupled device camera in a low-light level imaging system; f. determining the light intensity in the image using the low-light level imaging system; g. comparing the light intensity in the image with the pre-determined display intensity of the calibration phantom; h. deriving a fitted function that quantifies the relationship between the calibrated display intensity and the light intensity in the image reported by the low-light level imaging system; i. determining the spatial resolution of the system from observations of spatial frequency patterns presented on the TFT display image; j. determining the location of geometric control points presented on the TFT display image; and; k. comparing the location of the control points in the image with the pre-determined location of the control points in the phantom;

Metadata:
- Claim Count in Document: 9.0
- Percentile: 88.0
- Lexical Diversity: 1.62385
- Patent Class: 315.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['10997324', '12453834', '14343612', '10068573', '10870779']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6368415488489613
- 35 USC 102 Novelty (BERT): 0.4765043296536994
- Combined Prediction Score: 0.6208078269294351
- Mean Citation Score: 154.455552
- Max Citation Score: 163.53224
- Similarity Product: 125.311883760252

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test