PATENT CLAIM ANALYSIS

Application Number: 15755485
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-09
Patent Classification: ["435", "006100"]

Abstract:
The present invention provides a method for detecting the presence or absence of a target analyte in a test sample, comprising the steps: i) providing a plurality of detector nanoparticles comprising a first detector nanoparticle functionalised with a first probe specific for a first region of a target analyte and a second detector nanoparticle functionalised with a second probe specific for a second region of said target analyte; and ii) contacting said detector nanoparticles with a test sample under conditions suitable for the binding of the specific probes to the target analyte, wherein target analyte-induced agglomeration of the detector nanoparticles permits the release of a detectable signal means from a signal reservoir. Also provided are related devices and systems for performing the method of the invention.

Claim (Index 33):
A method for detecting the presence or absence of a target nucleic acid in a test sample, comprising the steps of:\n i) heat denaturation of a test sample; optionally, ii) providing and contacting an excess of an oligonucleotide blocking probe capable of specific binding to the antisense strand of a target nucleic acid with the test sample under conditions suitable for the binding of the blocking probes to the antisense strand of the target nucleic acid, thereby preventing the reannealing of sense and antisense strands of the target nucleic acid; iii) providing a plurality of detector nanoparticles functionalised with a first probe specific for a first region of the sense strand of a target nucleic acid and a second probe specific for a second region of the sense strand of the target nucleic acid; iv) contacting said detector nanoparticles with the test sample under conditions suitable for the binding of the specific probes to the target nucleic acid, wherein the presence of the target nucleic acid in the test sample results in agglomeration of the detector nanoparticles; v) irradiating the detector nanoparticles with a source of electromagnetic radiation configured to provide energy at the resonant wavelength of the detector nanoparticles when in an agglomerated state, thereby directing a heat transfer sufficient to cause at least partial thermal breakdown of a plurality of thermoresponsive microspheres containing a signal means; and vi) observing the release or lack thereof of the signal means from the microspheres, wherein the released signal means is captured for detection on a retention strip observable with the naked eye.

Metadata:
- Claim Count in Document: 20.0
- Percentile: 96.0
- Lexical Diversity: 2.06154
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15341162', '10008978', '10236205', '15036240', '10431341']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5786686739057492
- 35 USC 102 Novelty (BERT): 0.5182535708445185
- Combined Prediction Score: 0.5726271635996261
- Mean Citation Score: 277.60473599999995
- Max Citation Score: 306.76163
- Similarity Product: 220.21445845163825

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test