PATENT CLAIM ANALYSIS

Application Number: 16005502
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["435", "006100"]

Abstract:
The disclosure provides methods and kits for preparing sequencing library to detect chromosomal abnormality using cell-free DNA (cfDNA) without the need of first isolating the cfDNA from a liquid fraction of a test sample. In some embodiments, the method involves reducing the binding between the cfDNA and nucleosomal proteins without unwinding the cfDNA from the nucleosomal proteins. In some embodiments, the reduction of binding may be achieved by treating with a detergent or heating. In some embodiments, the method further involves freezing and thawing the test sample before reducing the binding between the cfDNA and the nucleosomal proteins. In some embodiments, the test sample is a peripheral blood sample from a pregnant woman including cfDNA of both a mother and a fetus. In other embodiments, the test sample is a peripheral blood sample from a patient known or suspected to have cancer.

Claim (Index 17):
The method of  claim 15 , wherein (c) comprises contacting the plasma fraction with a transposase and polynucleotides comprising a sequencing adapter sequence.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 94.0
- Lexical Diversity: 2.09459
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14214277', '14802873', '14802936', '12958353', '15072255']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5596103114956382
- 35 USC 102 Novelty (BERT): 0.6243062299674685
- Combined Prediction Score: 0.5660799033428212
- Mean Citation Score: 453.48631000000006
- Max Citation Score: 644.9162
- Similarity Product: 500.3868318391681

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test