PATENT CLAIM ANALYSIS

Application Number: 15865574
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["424", "093210"]

Abstract:
The technology described herein relates to methods and kits for directed differentation of primordial NKX2-1+ lung progenitors along proximal differentiation pathways into functional airway epithelial cells and airway organoids (“bronchospheres”) or along distal lineage pathways using modulation of Wnt signaling. Other aspects relate cell lines, methods, assays and kits comprising airway epithelial cells, and assays for diagnosing a disease that affects swelling of the bronchospheres, and/or for assessing genetic lesions and/or drugs for treating the the disease, where the disease is cystic fibrosis. Other aspects relate to personalized medicine and methods of treatment of cystic fibrosis using the airway epithelial cells.

Claim (Index 22):
An assay to select an agent that increases CTFR function, the assay comprising:\n a. contacting a population of airway epithelial cells with forskolin and a candidate agent, wherein the airway epithelial cells are any of: NKX2-1+/SOX2+ cells, NKX2-1+/P63+ cells or NKX2-1+/P63+/K5 cells, b. measuring the size of each airway epithelial cell at a first timepoint, where the first timepoint is prior to, or shortly after the airway epithelial cells are contacted with the forskolin and candidate agent, c. culturing the airway epithelial cells for a pre-defined period of time, d. measuring the size of each airway epithelial cell at a second timepoint, or a plurality of timepoints during the pre-defined period of time, wherein the second timepoint or plurality of timepoints is after the first timepoint, e. calculating the difference in the size of each airway epithelial cell measured at the second, or plurality of timepoints as compared to the first timepoint, and determining the average change in size of each airway epithelial cell in the population of airway epithelial cells, f. selecting the candidate agent as an agent that increases CFTR function where the average size of each airway epithelial cell is increased as compared to the average size of each airway epithelial cell in the absence of the candidate agent, or in the presence of a control candidate agent.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 86.0
- Lexical Diversity: 1.66176
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15635975', '14371627', '14367061', '15223603', '14417273']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7696259319160436
- 35 USC 102 Novelty (BERT): 0.5327442645914086
- Combined Prediction Score: 0.74593776518358
- Mean Citation Score: 358.088658
- Max Citation Score: 371.3459
- Similarity Product: 249.7387552989244

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test