PATENT CLAIM ANALYSIS

Application Number: 16123823
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2018-12
Patent Classification: ["435", "006120"]

Abstract:
In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

Claim (Index 1):
A method for paired-end nucleic acid bidirectional sequencing comprising:\n hybridizing a first soluble primer to a distal end of a nucleic acid template strand having a distal and proximal end, wherein the proximal end of the nucleic acid template strand is linked to a support; sequencing at least a portion of the nucleic acid template strand by extending the hybridized first soluble primer towards the proximal end of the nucleic acid template strand and the support, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template strand and obtaining a first sequencing read; introducing a site-specific nick into the proximal end of the nucleic acid template strand which is hybridized to the extended first primer product, wherein the nick includes a free 5\u2032 end and a free 3\u2032 end; degrading a portion of the nucleic acid template strand from the free 5\u2032 end of the nick using a degrading agent, thereby generating a single-stranded portion within the extended first primer product, wherein a portion of the extended first primer product remains hybridized to an un-degraded portion of the nucleic acid template strand; and sequencing at least a portion of the single-stranded first primer product by extending a primer on the single-stranded first primer product, thereby obtaining a second sequencing read.

Metadata:
- Claim Count in Document: 4.0
- Percentile: 97.0
- Lexical Diversity: 2.57407
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15730536', '13543521', '14832772', '15423744', '12982029']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7945215703745301
- 35 USC 102 Novelty (BERT): 0.8326760114128859
- Combined Prediction Score: 0.7983370144783657
- Mean Citation Score: 984.574944
- Max Citation Score: 1183.105
- Similarity Product: 1116.148338868022

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test