PATENT CLAIM ANALYSIS

Application Number: 16199681
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-03
Patent Classification: ["435", "325000"]

Abstract:
Methods, kits, compositions, and systems are provided for culturing pluripotent stem cells to produce populations of cells comprising beta-like cells (e.g., pancreatic lineage, glucose-responsive, and/or insulin-producing). In particular, culture conditions are provided that result in the generation of beta-like cells from a starting culture of human pluripotent stem cells.

Claim (Index 14):
A method of screening an agent, comprising:\n a) contacting a composition comprising cluster aggregates of pancreatic \u03b2-like cells, wherein said pancreatic \u03b2-like cells are generated by a method comprising:\n (i) culturing human pluripotent stem cells on an extracellular matrix for 2-4 days in a chemically defined medium, basic fibroblast growth factor, Activin A, and BMP4; \n (ii) culturing the cells on an extracellular matrix from step (a) for 2-4 days in the presence of chemically defined insulin, transferrin and selenium (ITS) medium, FGF7, and nicotinamide; \n (iii) culturing the cells on an extracellular matrix from step (b) for 3-5 days in the presence of a chemically defined ITS medium; retinoic acid; Noggin; and nicotinamide; and \n (iv) culturing the cells on an extracellular matrix from step (c) for 6-10 days in the presence of a B27 serum-free medium, IGF I, IGF II, FGF7, insulin, nicotinamide, exendin-4, an ALK5i II, and forskolin, thereby producing cluster aggregates of insulin+glucagon-pancreatic \u03b2-like cells that exhibit in vitro glucose stimulated insulin secretion \n with said agent; and b) assaying the effect of said agent on one or more properties or activities of said cluster aggregates.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 98.0
- Lexical Diversity: 1.46341
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15187345', '14858465', '15705896', '14263123', '13492244']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6699912997112961
- 35 USC 102 Novelty (BERT): 0.728064145148395
- Combined Prediction Score: 0.675798584255006
- Mean Citation Score: 652.5239560000001
- Max Citation Score: 824.9689999999997
- Similarity Product: 788.6964499343037

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test