PATENT CLAIM ANALYSIS

Application Number: 15874904
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2019-07
Patent Classification: ["435", "325000"]

Abstract:
A gene knockin method and a kit for gene knockin are provided. The method comprises (a) introducing a RNA-guided endonuclease that cleaves the chromosome at the insertion site into the cell; (b) introducing a guide RNA into the cell; and (c) introducing a donor plasmid into the cell, wherein the donor plasmid comprises the donor sequence flanked with a 5′ homology arm and a 3′ homology arm, a 5′ flanking sequence upstream of the 5′ homology arm, and a 3′ flanking sequence downstream of the 3′ homology arm, wherein the 5′ homology arm is homologous to a 5′ target sequence upstream of the insertion site on the genome and the 3′ homology arm is homologous to a 3′ target sequence downstream of the insertion site on the genome, wherein the guide RNA recognizes the insertion site, the 5′ flanking sequence, and the 3′ flanking sequence, wherein the RNA-guided endonuclease cleaves the donor plasmid at the 5′ flanking sequence and the 3′ flanking sequence, thereby producing a linear nucleic acid, wherein the donor sequence is inserted in to the genome at the insertion site through homology-directed repair.

Claim (Index 1):
A method of inserting a donor sequence at a predetermined insertion site on a genome in an eukaryotic cell, comprising introducing a RNA-guided endonuclease, a guide RNA and a donor plasmid into the cell, and introducing a combination of cyclin D1 and Nocodazole,\n wherein the donor plasmid comprises the donor sequence flanked with a 5\u2032 homology arm and a 3\u2032 homology arm, a 5\u2032 flanking sequence upstream of the 5\u2032 homology arm, and a 3\u2032 flanking sequence downstream of the 3\u2032 homology arm, wherein the 5\u2032 homology arm is homologous to a 5\u2032 target sequence upstream of the insertion site on the genome and the 3\u2032 homology arm is homologous to a 3\u2032 target sequence downstream of the insertion site on the genome, wherein the guide RNA recognizes the insertion site, the 5\u2032 flanking sequence, and the 3\u2032 flanking sequence, wherein the RNA-guided endonuclease cleaves the genome at the insertion site, wherein the RNA-guided endonuclease cleaves the donor plasmid at the 5\u2032 flanking sequence and the 3\u2032 flanking sequence to produce a linear nucleic acid, and wherein the donor sequence is inserted in to the genome at the insertion site through homology-directed repair.

Metadata:
- Claim Count in Document: 47.0
- Percentile: 86.0
- Lexical Diversity: 3.21875
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15701385', '15100105', '14319380', '13889162', '14577997']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6589094535512438
- 35 USC 102 Novelty (BERT): 0.5331940597134966
- Combined Prediction Score: 0.646337914167469
- Mean Citation Score: 370.796632
- Max Citation Score: 383.6589
- Similarity Product: 307.56685274584294

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test