PATENT CLAIM ANALYSIS

Application Number: 15765860
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-10
Patent Classification: ["435", "455000"]

Abstract:
The present invention provides methods and compositions to improve the efficiency of somatic cell nuclear transfer (SCNT) of human cells and the consequent production of human nuclear transfer ESC (hNT-ESCs). More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of human donor somatic cells prevents efficient human somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of hSCNT by exogenous or overexpression of the demethylase KDM4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases SUV39h1 and/or SUV39h2.

Claim (Index 4):
A method for producing a human nuclear transfer embryonic stem cell (hNT-ESC), comprising;\n a. at least one of: (i) contacting a donor human somatic cell or a recipient human oocyte with at least one agent which decreases H3K9me3 methylation in the donor human somatic cell or the recipient human oocyte; wherein the recipient human oocyte is a nucleated or enucleated oocyte; enucleating the recipient human oocyte if the human oocyte is nucleated; transferring the nuclei from the donor human somatic cell to the enucleated oocyte to form a hybrid oocyte; and\n activating the hybrid oocyte to form a human SCNT embryo; or\n (ii) contacting a hybrid oocyte with at least one agent which decreases \n \n H3K9me3 methylation in the hybrid oocyte, where the hybrid oocyte is an enucleated human oocyte comprising the genetic material of a human somatic cell, and activating the hybrid oocyte to form a human SCNT embryo; or\n (iii) contacting a human SCNT embryo after activation with at least one agent \n \n which decreases H3K9me3 methylation in the SCNT embryo, wherein the SCNT embryo is generated from the fusion of an enucleated human oocyte with the genetic material of a human somatic cell; \n b. incubating the SCNT embryo for a sufficient amount of time to form a blastocyst; and collecting at least one blastomere from the blastocyst and culturing the at least one blastomere to form at least one human NT-ESC.

Metadata:
- Claim Count in Document: 28.0
- Percentile: 91.0
- Lexical Diversity: 1.86154
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14276810', '12122557', '15895976', '14775594', '11968745']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5832966429222146
- 35 USC 102 Novelty (BERT): 0.5641115133493272
- Combined Prediction Score: 0.5813781299649259
- Mean Citation Score: 340.354036
- Max Citation Score: 444.93427
- Similarity Product: 370.5052593566871

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test