PATENT CLAIM ANALYSIS

Application Number: 15899206
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-07
Patent Classification: ["435", "006100"]

Abstract:
The present invention provides methods and materials related to the detection of colorectal neoplasm-specific markers (e.g., markers associated with colorectal cancer, markers associated with adenoma) in or associated with a subject's stool sample. In particular, the present invention provides methods and materials for identifying mammals (e.g., humans) having a colorectal neoplasm by detecting the presence and level of indicators of colorectal neoplasia such as, for example, long DNA (e.g., quantified by Alu PCR) and the presence and level of tumor-associated gene alterations (e.g., mutations in KRAS, APC, melanoma antigen gene, p53, BRAF, BAT26, PIK3CA) or epigenetic alterations (e.g., DNA methylation) (e.g., CpG methylation) (e.g., CpG methylation in coding or regulatory regions of bmp-3, bmp-4, SFRP2, vimentin, septin9, ALX4, EYA4, TFPI2, NDRG4, FOXE1) in DNA from a stool sample obtained from the mammal.

Claim (Index 2):
A method, comprising:\n a) extracting genomic DNA from a biological sample of a human individual suspected of having or having colorectal neoplasia, b) treating the extracted genomic DNA with bisulfite, c) amplifying the bisulfite-treated genomic DNA with primers consisting of a pair of primers specific for BMP3 and NDRG4, and d) measuring the methylation level of one or more CpG sites in BMP3 and NDRG4 by methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, quantitative bisulfite pyrosequencing, and bisulfite genomic sequencing PCR.

Metadata:
- Claim Count in Document: 3.0
- Percentile: 88.0
- Lexical Diversity: 2.08333
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15467739', '15724890', '15197105', '14168552', '14827013']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6572601058064486
- 35 USC 102 Novelty (BERT): 0.5281910623591349
- Combined Prediction Score: 0.6443532014617172
- Mean Citation Score: 368.246272
- Max Citation Score: 368.8257
- Similarity Product: 300.6508497518599

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test