PATENT CLAIM ANALYSIS

Application Number: 15881409
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-08
Patent Classification: ["435", "006120"]

Abstract:
Provided herein is technology for neoplasia screening, and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of cancer, in particular, colorectal cancer.

Claim (Index 110):
A method of characterizing a sample from a human subject, the method comprising assaying the sample for amounts of a plurality of different methylated marker DNAs and amounts of at least one control DNA, the method comprising:\n a) combining DNA from the sample in a pre-amplification reaction mixture comprising PCR amplification reagents, wherein the PCR amplification reagents comprise:\n i) a plurality of methylation marker primer pairs for amplifying target regions from a plurality of different methylated marker DNAs selected from the group of ANKRD13B; CHST2; CNNM1; DOCK2; DTX1; FER1L1; FERMT3; FLI1; GRIN2D; JAM3; LRRC4; OPLAH; PDGFD; PKIA; PPP2R5C; QKI; SEP9; SFMBT2; SLC12A8; TBX15; TSPYL5; VAV3; ZDHHC1; ZNF304; ZNF568; and ZNF671; and \n ii) at least one control primer pair for amplifying a target region from at least one control DNA; \n b) exposing the pre-amplification reaction mixture to thermal cycling conditions wherein a plurality of different methylation marker target regions and at least one control DNA target region are amplified to produce a pre-amplified mixture; c) partitioning the pre-amplified mixture into a plurality of assay reaction mixtures, wherein each assay reaction mixture comprises:\n i) additional amounts of at least one methylation marker primer pair used in step a) i) and of the at least one control primer pair; and \n ii) oligonucleotides hybridizable to the methylation marker target regions and control DNA target regions amplifiable with the primer pairs of c) i); \n d) exposing the plurality of assay reaction mixtures to thermal cycling conditions wherein amplification of a plurality of different methylated marker target regions and at least one control DNA target region occurs; and e) detecting amplification of a plurality of different methylated marker target regions and at least one control DNA target region in the assay reaction mixtures.

Metadata:
- Claim Count in Document: 324.0
- Percentile: 86.0
- Lexical Diversity: 1.33333
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15471337', '15841006', '14966938', '15252966', '14669834']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6078648701440706
- 35 USC 102 Novelty (BERT): 0.5440427475604119
- Combined Prediction Score: 0.6014826578857048
- Mean Citation Score: 348.420024
- Max Citation Score: 412.8369
- Similarity Product: 323.80529147568944

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test