PATENT CLAIM ANALYSIS

Application Number: 16144059
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-01
Patent Classification: ["435", "007400"]

Abstract:
The present invention pertains to a method for the generation of neurotoxin-sensitive, neuronal differentiated cells comprising the steps of: a) cultivating tumor cells which are able to differentiate into neuronal cells in a culture medium under conditions and for a time which primes said tumor cells for neuronal differentiation; and b) cultivating the tumor cells primed for neuronal differentiation of a) in a differentiation medium having an osmolality of 100 to 270 mOsm/kg, and comprising (i) B27 supplement and/or (ii) N2 supplement, for at least 3 days, thereby obtaining neurotoxin-sensitive, neuronal differentiated cells. The invention further relates to neurotoxin-sensitive, neuronal differentiated cells obtainable by the method of the invention. In addition, the invention encompasses a method for determining the activity of a neurotoxin polypeptide comprising the steps of: a) contacting the neurotoxin-sensitive, neuronal differentiated cells obtainable by the method of the invention with a neurotoxin polypeptide; b) cultivating the neurotoxin-sensitive, neuronal differentiated cells of step a) for 3 to 74 hours or 72 hours under conditions which allow for the neurotoxin polypeptide to exert its biological activity; and c) determining the activity of the neurotoxin polypeptide in the said cells after cultivation according to step b). Finally, the invention provides for a medium comprising OptiMEM, FBS, B27 supplement, and N2 supplement.

Claim (Index 8):
The method of  claim 1 , wherein step a) comprises reduction of serum from the culture medium and/or addition of retinoic acid.

Metadata:
- Claim Count in Document: 29.0
- Percentile: 97.0
- Lexical Diversity: 2.72222
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['14435583', '13779972', '15528219', '13829783', '14214598']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.601978068428893
- 35 USC 102 Novelty (BERT): 0.633075532529977
- Combined Prediction Score: 0.6050878148390013
- Mean Citation Score: 319.7465880000001
- Max Citation Score: 632.563
- Similarity Product: 542.0420888696908

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test