PATENT CLAIM ANALYSIS

Application Number: 15753317
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["506", "004000"]

Abstract:
Systems, kits, and methods for detecting and quantifying proteomic activity using DNA-encoded probes are provided, where the proteomic activity may be enzymatic activity or ligand binding affinity. Such systems and methods encode quantitative proteomic activity information into DNA sequence populations and utilize DNA-linked substrates or ligands as activity probes. The systems, kits, and methods that are directed to detecting ligand affinity further include crosslinking steps to ensure the integrity of the DNA-linked ligands during purification and washing. Signal detection involves the chemical manipulation of a probe population downstream of sample exposure and application of purifying, selective pressure for desired products. Selection-induced changes in DNA abundance between the initial pool and the purified pool indicate sample activity.

Claim (Index 12):
A method for detecting proteomic activity in a sample, the method comprising the steps of:\n providing a population of probes, each of the probes comprising a substrate linked to an DNA construct, the DNA construct comprising one or more amplifiable identification barcode regions and the substrate specific to a target protein, wherein at least one of the barcode regions of the double-stranded DNA is unique; contacting the population of probes with a sample comprising a target protein in an initial pool and under conditions and for a sufficient time to allow the target protein to turnover the substrate of a probe into a product such that the DNA construct of such probe encodes the product; quenching enzyme activity within the initial pool; isolating the DNA constructs of the probes of the initial pool into a DNA pool; separating the DNA constructs of the DNA pool that encode the product to form a purified pool; quantifying the DNA constructs of the DNA pool and the DNA constructs of the purified pool; and detecting the presence or absence of a detectable signal, the detectable signal comprising identifying a change in probe frequency between the DNA pool and the purified pool.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 88.0
- Lexical Diversity: 1.60759
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14239369', '15080767', '14786185', '14900604', '14346913']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5609382944876928
- 35 USC 102 Novelty (BERT): 0.5026752730246213
- Combined Prediction Score: 0.5551119923413856
- Mean Citation Score: 231.68102
- Max Citation Score: 239.79802
- Similarity Product: 159.14482318687442

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test