PATENT CLAIM ANALYSIS

Application Number: 16231185
Application Type: Utility
Filing Date: 2018-12
Publication Date: 2019-06
Patent Classification: ["435", "006120"]

Abstract:
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Claim (Index 30):
A method of single cell gene expression analysis, comprising:\n (a) providing a plurality of beads, wherein a bead of said plurality of beads comprises a plurality of nucleic acid barcode molecules each comprising:\n (i) a common barcode sequence configured to identify a cellular origin of mRNA molecules; and \n (ii) an identification barcode sequence configured to quantify mRNA molecules; \n (b) partitioning a plurality of cells and said plurality of beads in a system comprising a plurality of partitions, wherein said system comprises 1,000 occupied partitions each comprising a single cell of said plurality of cells and a single bead of said plurality of beads, wherein different occupied partitions have different common barcode sequences; (c) releasing mRNA molecules from single cells of said 1,000 occupied partitions; (d) using said plurality of nucleic acid barcode molecules to generate a plurality of barcoded nucleic acid molecules from the mRNA molecules released in (c), wherein a barcoded nucleic acid molecule of said plurality of barcoded nucleic acid molecules comprises: (i) a sequence of an mRNA molecule of said released mRNA molecules, or a complement of said sequence of said mRNA molecule; and (ii) a sequence of a nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules, including said common barcode sequence and said identification barcode sequence, or a complement of said sequence of said nucleic acid barcode molecule; (e) determining sequences of said plurality of barcoded nucleic acid molecules; (f) using common barcode sequences from said sequences determined in (f) to identify cellular origins of said plurality of barcoded nucleic acid molecules, thereby identifying cellular origins of said mRNA molecules from which said plurality of barcoded nucleic acid molecules were generated; and (g) using identification barcode sequences from said sequences determined in (f) to identify molecular origins of said plurality of barcoded nucleic acid molecules, thereby quantifying said mRNA molecules from which said plurality of barcoded nucleic acid molecules were generated.

Metadata:
- Claim Count in Document: 89.0
- Percentile: 98.0
- Lexical Diversity: 1.33333
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['16052431', '16144832', '15847752', '15980473', '16138448']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6468367043246096
- 35 USC 102 Novelty (BERT): 0.5606678743616668
- Combined Prediction Score: 0.6382198213283153
- Mean Citation Score: 446.878004
- Max Citation Score: 464.13983
- Similarity Product: 376.37573393710136

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test