PATENT CLAIM ANALYSIS

Application Number: 16079499
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2019-02
Patent Classification: ["435", "171000"]

Abstract:
The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.

Claim (Index 18):
The method of  claim 16 , wherein the reaction mixture comprises:\n (a) the recombinant DAP1 polypeptide having at least 60% sequence identity to the amino acid sequence set forth in SEQ ID NO:2; the recombinant polypeptide capable of synthesizing geranylgeranyl pyrophosphate (GGPP) from farnesyl diphosphate (FPP) and isopentenyl diphosphate (IPP); the recombinant polypeptide capable of synthesizing ent-copalyl diphosphate from GGPP; the recombinant polypeptide capable of synthesizing ent-kaurene from ent-copalyl pyrophosphate; the recombinant polypeptide capable of synthesizing ent-kaurenoic acid, ent-kaurenol, and/or ent-kaurenal from ent-kaurene and having at least 70% sequence identity to one of the amino acid sequences set forth in SEQ ID NO:60, SEQ ID NO:62, SEQ ID NO:117, SEQ ID NO:66, SEQ ID NO:68, SEQ ID NO:70, SEQ ID NO:72, SEQ ID NO:74, or SEQ ID NO:76; the recombinant polypeptide capable of synthesizing steviol from ent-kaurenoic acid and having at least 70% sequence identity to one of the amino acid sequences set forth in SEQ ID NO:94, SEQ ID NO:97, SEQ ID NO:100, SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, SEQ ID NO:104, SEQ ID NO:106, SEQ ID NO:108, SEQ ID NO:110, SEQ ID NO:112, or SEQ ID NO:114; the recombinant polypeptide capable of reducing cytochrome P450 complex and having at least 70% sequence identity to one of the amino acid sequences set forth in SEQ ID NO:78, SEQ ID NO:80, SEQ ID NO:82, SEQ ID NO:84, SEQ ID NO:86, SEQ ID NO:88, SEQ ID NO:90, SEQ ID NO:92; the recombinant polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl group; the recombinant polypeptide capable of beta 1,3 glycosylation of the C3\u2032 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; the recombinant polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl group; and/or the recombinant polypeptide capable of beta 1,2 glycosylation of the C2\u2032 of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside; (b) glucose, fructose, sucrose, xylose, rhamnose, uridine diphosphate (UDP)-glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and/or (c) reaction buffer and/or salts.

Metadata:
- Claim Count in Document: 11.0
- Percentile: 96.0
- Lexical Diversity: 1.13333
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15506196', '15541686', '14761629', '15382354', '14237540']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7438103901273456
- 35 USC 102 Novelty (BERT): 0.6420061635077774
- Combined Prediction Score: 0.7336299674653889
- Mean Citation Score: 585.9904920000001
- Max Citation Score: 643.51636
- Similarity Product: 501.104651464467

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test