PATENT CLAIM ANALYSIS

Application Number: 15731991
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2019-03
Patent Classification: ["424", "094200"]

Abstract:
The Cloning and Expression of Comosain's Minigene and the Methods and Compositions for treating and/or preventing various types of cancer which comprise administered an effective amount of Glyco-polypeptides and Bioflavonoids such as Comosain, Ananase, Bromelainases, Trypsin, Pepsin, Quercetin, Rutin, Naringenin, Genistein, Hesperetin, etc and/or a mixture thereof.

Claim (Index 8):
8 ). Assembly of expression vector for the Comosain's minigene and preparation of genetically modified organism (GMO). For direct expression of the genomic Comosain's gene, 4.8 kilobase (kb) of BstTy/Se- and BamAs/GL fragment of Comosain, which contains the entire gene. after converting the BstTy/Se (tyrosine-serine 10 amino acids)site into Bst As/GL(asparagine-to-glycine 20 amino acids) site with a synthetic linker(pBR322 ori) the fragment was insert into the unique BamAs/GL site of the expression vector pDSVL, which contains a dihydrofolate reductase (DHFR) minigene. The resulting Plasmid DSVL-gPlCOS (gene Plant Comosain) was then used to transfect New Zealand white rabbit ovarian (NWRO) cells by the calcium phosphate microprecipitate method. The transformants were selected by the medium lacking hypoxanthine and thymidine. The culture medium used was Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, penicillin, streptomycin, and glutamine.

Metadata:
- Claim Count in Document: 13.0
- Percentile: 95.0
- Lexical Diversity: 1.37209
- Patent Class: 424.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15272826', '14003629', '12861152', '11214831', '10617943']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7135330018742991
- 35 USC 102 Novelty (BERT): 0.5101592823034181
- Combined Prediction Score: 0.6931956299172111
- Mean Citation Score: 172.64342000000005
- Max Citation Score: 186.47795
- Similarity Product: 100.26009008917214

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test