PATENT CLAIM ANALYSIS

Application Number: 16039123
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2019-01
Patent Classification: ["435", "069600"]

Abstract:
The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.

Claim (Index 21):
A method for upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture process comprising:\n establishing a mammalian cell culture in a bioreactor with a cell culture media that does not contain mannose; growing the mammalian cells during a growth phase with a cell culture media that does not contain mannose; inducing cell growth-arrest by L-asparagine starvation with a serum-free media that does not contain mannose, followed by perfusion with a serum-free perfusion media that does not contain mannose and having an L-asparagine concentration of 5 mM or less; initiating and maintaining a production phase in the cell culture by perfusion with a serum-free perfusion media containing mannose, wherein the mannose to total hexose ratio in the perfusion media is greater than 0 but less than 1.0.

Metadata:
- Claim Count in Document: 56.0
- Percentile: 95.0
- Lexical Diversity: 1.27586
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14289943', '15285392', '14776404', '15115615', '15111470']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6595085570387597
- 35 USC 102 Novelty (BERT): 0.6419517890439365
- Combined Prediction Score: 0.6577528802392774
- Mean Citation Score: 474.1191400000001
- Max Citation Score: 641.5584
- Similarity Product: 579.1115987909318

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test