PATENT CLAIM ANALYSIS

Application Number: 15750824
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-10
Patent Classification: ["506", "002000"]

Abstract:
Reagents and methods for the analysis of nucleic acids (e.g. genomic DNA) of circulating microparticles (i.e. microparticles originating from blood) are provided. The methods comprise linking at least two fragments of a target nucleic acid of a circulating microparticle to produce a set of at least two linked fragments of the target nucleic acid. In the methods, fragments of a target nucleic acid may be linked by techniques such as barcoding, partitioning, ligation and/or separate sequencing. The sequencing of a set of linked fragments provides a set of informatically linked sequence reads corresponding to the sequences of fragments from a single microparticle.

Claim (Index 62):
A method of preparing a sample for sequencing, wherein the sample comprises first and second circulating microparticles, and wherein each circulating microparticle contains at least two fragments of a target nucleic acid, and wherein the method comprises the steps of:\n (a) contacting the sample with a library comprising at least two multimeric barcoding reagents, wherein each multimeric barcoding reagent comprises first and second barcode regions linked together, wherein each barcode region comprises a nucleic acid sequence and wherein the first and second barcode regions of a first multimeric barcoding reagent are different to the first and second barcode regions of a second multimeric barcoding reagent of the library; and (b) appending barcode sequences to each of first and second fragments of the target nucleic acid of the first circulating microparticle to produce first and second barcoded target nucleic acid molecules for the first circulating microparticle, wherein the first barcoded target nucleic acid molecule comprises the nucleic acid sequence of the first barcode region of the first multimeric barcoding reagent and the second barcoded target nucleic acid molecule comprises the nucleic acid sequence of the second barcode region of the first multimeric barcoding reagent, and appending barcode sequences to each of first and second fragments of the target nucleic acid of the second circulating microparticle to produce first and second barcoded target nucleic acid molecules for the second circulating microparticle, wherein the first barcoded target nucleic acid molecule comprises the nucleic acid sequence of the first barcode region of the second multimeric barcoding reagent and the second barcoded target nucleic acid molecule comprises the nucleic acid sequence of the second barcode region of the second multimeric barcoding reagent.

Metadata:
- Claim Count in Document: 44.0
- Percentile: 88.0
- Lexical Diversity: 1.94915
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15842687', '14990276', '14006971', '14316447', '14947988']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.534194504618003
- 35 USC 102 Novelty (BERT): 0.5363046220246598
- Combined Prediction Score: 0.5344055163586687
- Mean Citation Score: 344.23017
- Max Citation Score: 360.96902
- Similarity Product: 269.5699233483601

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test