PATENT CLAIM ANALYSIS

Application Number: 16169376
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-02
Patent Classification: ["435", "188000"]

Abstract:
A series of protein A mutants having high alkali resistance, and methods of using the protein A mutants are provided. The protein A mutants have a high binding affinity for regions of immunoglobulin proteins other than the complementarity determining regions. The protein A mutants can be coupled to a solid support for immunoglobulin isolation, or conjugated to a label for immunoglobulin detection. This series of protein A mutants have high chemical stability under alkaline conditions of pH 13-14, and can also be used as chromatography ligands for purification procedures that use alkaline solutions under harsh conditions, such as Clean-In-Place (CIP). Also provided are methods of immunoglobublin separation and purification, and alkali regeneration of affinity chromatography medium that uses protein A as a ligand.

Claim (Index 17):
A multimer of a recombinantly expressed mutant protein A molecule comprising at least two recombinant expressed mutant protein A molecules for isolating, purifying, or detecting an immunoglobulin, the mutant protein A molecule comprising the amino acid sequence of SEQ ID NO:1, the amino acid sequence of SEQ ID NO:2, the amino acid sequence of residues 7 to 54 of SEQ ID NO:1, or the amino acid sequence of residues 7 to 54 of SEQ ID NO:2, wherein the recombinantly expressed mutant protein A molecule comprises an amino acid substitution at an amino acid position selected from the group consisting of amino acid position 7, 10, 33, 44, 46, 47, 48, and 50 corresponding to the wild type protein A sequence.

Metadata:
- Claim Count in Document: 23.0
- Percentile: 97.0
- Lexical Diversity: 1.875
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14778395', '14845817', '14961164', '12280221', '12900958']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6261655146018676
- 35 USC 102 Novelty (BERT): 0.600795420100401
- Combined Prediction Score: 0.623628505151721
- Mean Citation Score: 326.258792
- Max Citation Score: 517.2193
- Similarity Product: 498.0046515416623

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test