PATENT CLAIM ANALYSIS

Application Number: 15940523
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-10
Patent Classification: ["424", "186100"]

Abstract:
Non-human animals with humanized immunoglobulin loci and methods of using them in vaccine design are described, as well as methods for making broadly neutralizing antibodies against infectious agents and pathogens are provided. Non-human animals with humanized immunoglobulin loci used in B-cell-lineage immunogen design in vaccine development are provided, as are methods of carrying out such design.

Claim (Index 30):
A method for screening for a broad spectrum antibody against an antigen of interest, comprising:\n (a) administering a first immunogen derived from the antigen of interest to a mouse from a mouse line comprising in its germline:\n (i) a restricted immunoglobulin heavy chain locus comprising a single human unrearranged V H  gene segment, one or more human D H  gene segments, and one or more human J H  gene segments operably linked to a heavy chain constant region nucleic acid sequence, \n (ii) a genetically modified immunoglobulin light chain locus comprising one or more human V K  gene segments and one or more human JK gene segments, wherein the V K  and the J K  gene segments are operably linked to a light chain constant region nucleic acid sequence, and wherein the first immunogen comprises a plurality of epitopes; \n (b) allowing the mouse to mount an immune response against the first immunogen, wherein the immune response comprises generation of mouse B cells that express human immunoglobulin heavy chain (IgH) VDJ and human immunoglobulin light chain (IgL) VJ sequences; (c) isolating clonally related B cells from the mouse that express a B cell receptor (BCR) that specifically binds a first epitope of the first immunogen; (d) determining IgH VDJ and IgL VJ amino acid sequences of the B cell receptor (BCR) of the clonally related B cells; (e) deducing from the IgH VDJ and IgL VJ sequences unmutated B cell receptor (BCR) VDJ and VJ amino acid sequences; and one or more intermediate ancestor B cell receptor (BCR) VDJ and VJ amino acid sequences of the B cells at an intermediate stage of differentiation; (f) providing a plurality of second immunogens comprising second epitopes distinct from the first epitope that bind with enhanced affinity to the unmutated B cell receptor (BCR) or the intermediate ancestor B cell receptor (BCR) relative to the first immunogen; (g) serially administering to another mouse of the line second immunogens selected from the plurality of the second immunogens in (f), the serial administration starting with a second immunogen to the unmutated BCR and following with second immunogens to intermediate BCRs successively more distant phylogenically from the unmutated BCR; and (h) determining whether the immune response from the mouse of (g) includes a broad spectrum antibody, which binds a plurality of the second epitopes.

Metadata:
- Claim Count in Document: 23.0
- Percentile: 90.0
- Lexical Diversity: 1.62162
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14174563', '14682859', '13890519', '14192051', '13404075']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7173061142922453
- 35 USC 102 Novelty (BERT): 0.6737630966400132
- Combined Prediction Score: 0.7129518125270221
- Mean Citation Score: 501.68856399999993
- Max Citation Score: 730.8605299999997
- Similarity Product: 629.8152330904803

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test