PATENT CLAIM ANALYSIS

Application Number: 15901674
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-06
Patent Classification: ["435", "325000"]

Abstract:
This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.

Claim (Index 1):
A method for purifying intact cells from a biological sample, comprising:\n (a) providing a column comprised of a column body, a top frit and a bottom frit, wherein the column body, top frit and bottom frit define a media chamber, wherein the media chamber contains a loosely packed bed of medium, wherein the loosely packed bed of medium has a bed volume,\n (i) wherein the loosely packed bed of medium is comprised of beads, wherein the loosely packed bed of medium is packed in such a way that beads are not compressed, wherein the loosely packed bed of medium has one or more unrestricted flow paths, \n (ii) wherein the frits are comprised of pores having a pore size, wherein the pore size is large enough for cells to pass through and wherein the pore size is small enough to retain the loosely packed bed of medium; \n (b) providing a biological sample having a sample volume, wherein the sample volume is larger than the bed volume, wherein the biological sample is comprised of intact cells, wherein the intact cells comprise viable cells, and (c) passing the biological sample through the column, wherein the biological sample is passed through the column in a flowing stream, wherein the flowing stream is passed back and forth through the loosely packed bed of medium multiple times, wherein the cells travel through the unrestricted flow paths in the loosely packed bed of medium, whereby a portion of the intact cells are captured on the loosely packed bed of medium, wherein the biological sample is passed through the column at a rate of at least 0.01 ml/min.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 88.0
- Lexical Diversity: 1.76271
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15098275', '14563994', '15167752', '14181656', '13752227']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6226217812927507
- 35 USC 102 Novelty (BERT): 0.5309123242639262
- Combined Prediction Score: 0.6134508355898682
- Mean Citation Score: 288.830064
- Max Citation Score: 372.7041
- Similarity Product: 278.01066434887645

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test