PATENT CLAIM ANALYSIS

Application Number: 15894107
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-06
Patent Classification: ["435", "377000"]

Abstract:
This invention relates, e.g., to a method for differentiating mammalian (e.g., human) pluripotent stem cells (PSCs) into endothelial cells (ECs) in vitro, by plating a single-cell suspension of PSCs onto a suitable surface such as type IV collagen and culturing the cells with VEGF after which ECs can be harvested. A preferred embodiment of the method first cultures the cells without VEGF and then sequentially cultures the cells with VEGF. Differentiation can be enhanced by adding an inhibitor of transforming growth factor β to the culturing with VEGF.

Claim (Index 14):
A method for differentiating mammalian pluripotent stem cells (PSCs) into endothelial cells (ECs) in vitro, comprising the steps of:\n plating a single-cell suspension of PSCs onto a surface; culturing the cells without VEGF; culturing the cells in the presence of VEGF and a transforming growth factor-beta (TGF-\u03b2) inhibitor, wherein should intermediate Flk-1+ progenitor cells be produced after plating of the PSCs onto a surface they are not isolated from the culture prior to differentiation to ECs; and harvesting the cultured cells whereby ECs are produced.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 88.0
- Lexical Diversity: 1.57812
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14777258', '13844313', '13581341', '14897619', '15637068']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5367392832927558
- 35 USC 102 Novelty (BERT): 0.5302608089895405
- Combined Prediction Score: 0.5360914358624342
- Mean Citation Score: 296.55038
- Max Citation Score: 350.06534
- Similarity Product: 288.8237027056026

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test