PATENT CLAIM ANALYSIS

Application Number: 16282943
Application Type: Utility
Filing Date: 2019-02
Publication Date: 2019-06
Patent Classification: ["435", "007900"]

Abstract:
The invention relates to immunoassays which allow the detection of polypeptides in samples with a higher sensitivity than assays of the state of the art. The invention also relates to kits which provide the components needed for carrying out said immunoassays.

Claim (Index 46):
A method for determining or detecting the amount of a target polypeptide selected from the group consisting of A\u03b242, A\u03b240, and a mixture thereof in a plasma sample from a subject to be tested comprising the steps of:\n (i) capturing the target polypeptide present in the plasma sample with a first antibody which specifically binds said target polypeptide, wherein the first antibody is prebound to a solid support wherein the first antibody prebound to the solid support has been treated with a concentrated trehalose solution, and wherein the first antibody is directed against an epitope located within amino acids 1 to 16 of the N-terminal region of A\u03b240 and A\u03b242; (ii) contacting an immune complex formed in step (i) with at least one second antibody selected from a A\u03b242 antibody specific for a peptide consisting of amino acid residues of SEQ ID NO: 1 or SEQ ID NO: 2, a A\u03b240 antibody specific for a peptide consisting of amino acid residues of SEQ ID NO: 3, or a combination thereof which recognises a region of the target polypeptide which is different from the region that is recognised by the first antibody, wherein the at least one second antibody recognizes amino acid residues of the C-terminal of the target polypeptide; (iii) contacting the complexes formed in step (ii) with a reagent wherein the reagent binds to the second antibody and is coupled to a first member of a binding pair; (iv) contacting the complexes formed in step (iii) with a second member of a binding pair wherein the second member of a binding pair is coupled to a fluorescent, luminescent or enzyme tag; and (v) detecting or determining the activity or amount of tag attached to the second member of the binding pair, wherein the binding pair is selected from the group consisting of: hapten and antibody; antigen and antibody; biotin and avidin; biotin and streptavidin; a biotin analogue and avidin; a biotin analogue and streptavidin; sugar and lectin; enzyme and cofactor; and nucleic acid and complementary nucleic acid.

Metadata:
- Claim Count in Document: 20.0
- Percentile: 99.0
- Lexical Diversity: 1.3871
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14004634', '14977769', '13491810', '12294438', '13544600']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.641296864195895
- 35 USC 102 Novelty (BERT): 0.5312248773843711
- Combined Prediction Score: 0.6302896655147426
- Mean Citation Score: 295.8666100000001
- Max Citation Score: 373.2784
- Similarity Product: 282.66749764862067

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test