PATENT CLAIM ANALYSIS

Application Number: 16011460
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-04
Patent Classification: ["800", "008000"]

Abstract:
The invention provides methods for isolating cell-type specific mRNAs by selectively isolating ribosomes or proteins that bind mRNA in a cell type specific manner, and, thereby, the mRNA hound to the ribosomes or proteins that bind mRNA. Ribosomes, which are riboprotein complexes, bind mRNA that is being actively translated in cells. According to the methods of the invention, cells are engineered to express a molecularly tagged ribosomal protein or protein that binds mRNA by introducing into the cell a nucleic acid comprising a nucleotide sequence encoding a ribosomal protein or protein that binds mRNA fused to a nucleotide sequence encoding a peptide tag. The tagged ribosome or mRNA binding protein can then be isolated, along with the mRNA bound to the tagged ribosome or mRNA binding protein, and the mRNA isolated and further used for gene expression analysis. The methods of the invention facilitate the analysis and quantification of gene expression in the selected cell type present within a heterogeneous cell mixture, without the need to isolate the cells of that cell type as a preliminary step.

Claim (Index 79):
A non-human mammalian cell comprising:\n (1) a first nucleic acid comprising a transactivator-responsive conditional regulatory element and a first coding sequence, wherein the first coding sequence encodes a ribosomal fusion protein comprising a ribosomal protein, or fragment thereof, fused to a peptide tag, and wherein the first coding sequence is operably linked to the transactivator-responsive conditional regulatory element; and (2) a second nucleic acid comprising a mammalian endogenous promoter that directs gene expression in the non-human mammalian cell and a second coding sequence, wherein the second coding sequence encodes the transactivator, and wherein the second coding sequence is operably linked to the mammalian endogenous promoter; wherein the mammalian endogenous promoter controls expression of the transactivator in the non-human mammalian cell, the transactivator controls expression of the ribosomal fusion protein, and the ribosomal fusion protein is incorporated in an intact ribosome that translates and/or binds mRNA.

Metadata:
- Claim Count in Document: 44.0
- Percentile: 94.0
- Lexical Diversity: 2.22093
- Patent Class: 800.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13104316', '10494248', '14553937', '12049862', '14890705']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6009827724499894
- 35 USC 102 Novelty (BERT): 0.587707671583846
- Combined Prediction Score: 0.5996552623633751
- Mean Citation Score: 358.377196
- Max Citation Score: 552.68445
- Similarity Product: 445.17129831471743

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test