PATENT CLAIM ANALYSIS

Application Number: 15760836
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-12
Patent Classification: ["435", "006110"]

Abstract:
The invention relates to a method for confirming an amplified nucleic acid target sequence (target sequence), preferably from human samples, during a multiplication reaction in a collective and continuous reaction batch as a one-pot process, wherein the confirmation of the target sequence amplification product is obtained by means of a hapten-pair-marked artificial template amplification product. The artificial template sequence is amplified and optionally marked by means of the 5′-cleavage products of the at least one target-sequence-specific FEN probe. The 5′-cleavage product of the at least one target-sequence-specific FEN probe is obtained only if the FEN probe hybridizes, by means of the target-sequence-specific 3′ sequence thereof, to a complementary sequence segment of the at least one target sequence. The detection of the obtained plurality of template amplification products occurs distinctly and preferably by means of immunochromatographic methods.

Claim (Index 20):
A reaction mixture comprising:\n at least two target sequence-specific primers (P1, P2, P 1-n ) being suitable for amplification of at least one target sequence to be verified, at least one labelled target sequence-specific flap endonuclease probe (FEN probe FEN1, FEN 1-n ), wherein the at least one FEN probe comprises:\n a target sequence-specific 3\u2032-sequence which is complementary to a sequence fragment of the at least one target sequence within a region being restricted on the target sequence by the at least first primer (P1) and the at least second primer (P2), \n a protective group at the 3\u2032-end of the target sequence-specific 3\u2032-sequence, and \n a target sequence-unspecific 5\u2032-sequence which is labelled at its 5\u2032-end with one hapten of a specific hapten pair, and \n at least one artificial matrix sequence.

Metadata:
- Claim Count in Document: 25.0
- Percentile: 90.0
- Lexical Diversity: 2.13043
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['11150775', '11106237', '11607341', '11476893', '11286573']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5762666603349028
- 35 USC 102 Novelty (BERT): 0.5113936905233681
- Combined Prediction Score: 0.5697793633537493
- Mean Citation Score: 266.865404
- Max Citation Score: 275.5055
- Similarity Product: 192.3953610194028

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test