PATENT CLAIM ANALYSIS

Application Number: 15767551
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-10
Patent Classification: ["435", "205000"]

Abstract:
The present invention relates to glucoamylase variants having an increase in specific activity and/or an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, at least 90%, at least 95% identity, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Claim (Index 2):
The glucoamylase variants according to  claim 1 , comprising a substitution at one or more positions selected from the group consisting of: Q1K, Q1R, S2E, S2K, S2L, S2P, S2R, V3L, V3G, V3R, D4R, D4S, D4G, D4A, D4W, S5L, S5V, S5G, S5C, S5R, S8Q, S8H, S8A, S8Y, S9C, S9Q, S9M, S9W, S9D, S9G, I13V, I13R, I13S, I13L, I13E, K15G, K15R, V18M, V18Q, L19G, L19F, N25S, N25A, S27A, S27L, S27G, S27V, S27C, K28C, K28R, S30Q, S30A, S30K, S30T, S30L, V36K, V36G, V36W, V36A, V36I, V37R, V37K, V37G, V37C, V37M, V37S, V37T, V37D, T43K, D45L, D45P, S57P, S57L, S57G, S57F, S57R, S57T, S57A, V59T, V59S, V59E, F60S, I71M, I71S, I71T, I71V, S73H, S73R, S73N, S73V, S73G, T74V, L77S, L77P, L77R, D82N, D82R, D82V, D82G, D83L, D83C, D83W, V85Q, V85G, V85P, T86R, T86V, E88Q, E88R, E88G, L91S, L91P, L91G, S95A, S95T, S95V, P97T, P97I, P97R, T103Y, T103A, T103G, D114G, D114N, D114M, D114R, D114C, S134P, S134A, S134V, S134W, S134D, S134H, S134L, S134G, L137W, L137S, L137A, L137V, L137G, L137D, L137R, L137P, T139D, T139P, T139V, N142Y, N142H, N142C, L145C, L145D, L145G, L145V, L145S, S146W, S146L, S146R, S146G, S146P, N147Q, N147V, N147L, N147K, L147D, L147Y, L147H, L147S, N149H, N149T, N149R, N149K, N149S, Y152S, Y152A, Y152R, Y152L, Y152K, Y152E, Y152P, Y152V, Y152I, Y152C, Y152W, V153E, V153S, V153G, V153W, V153Y, T154N, T154R, T154K, T154P, T154V, S155C, S155P, S155R, S155G, S155A, N156I, N156A, N156V, N156R, N156T, N156K, L157P, L157R, L157A, L157G, L157W, W158T, W158A, W158M, W158V, W158R, W158P, P159S, P159G, P159L, P159V, P159A, P159R, P159Q, P159E, I160A, I160G, I160N, I160T, I160R, I160V, Q162L, Q162V, Q162H, Q162P, Q162R, V169A, V169L, V169W, V169S, V169D, V169R, V169E, S170A, S170P, S170R, S170M, S175L, S175C, S175W, T176R, T176L, T176N, T176A, T176S, T176I, Y177H, D184P, D184W, D184S, D184Y, D184G, S186A, S186R, S186W, R199F, R199E, R199L, R199C, R199K, A202R, A202W, A202E, A202S, A202V, A203M, A203W, A203P, A203L, T206C, T206P, T206G, T206A, T206R, Q210C, Q210G, Q210S, Q210R, Q210L, Q210P, Q210V, T211R, T211A, T211H, T211K, T211Q, T211G, T211W, T211E, T211I, T211V, T211P, T211L, T211D, S212D, S212E, S212L, S212P S212T, Q213W, Q213V, Q213D, Q213A, Q213T, Q213R, Q213G, Q213S, V214G, V214R, V214W, V214A, V214I, S215R, S215G, S215L, S215Y, S215P, S215E, S215W, Y217G, Y217C, Y217A, Y217S, Y217T, Y217F, T218H, T218C, T218A, T218M, T218Q, T218G, T219R, T219D, T219S, T219G, T219C, Q220R, Q220V, Q220D, Q220S, Q220L, A221V, A221T, A221L, A221P, A221R, A221E, D222V, D222W, D222T, D222G, D222L, D222R, D222N, D222F, D222M, N223A, N223S, N223R, N223F, N223P, N223G, N223L, L224G, L224D, L224K, L224V, L224R, F227G, F227W, Y231S, Y231T, Y231R, Y231L, Y231A, Y231V, Y231N, P234D, P234L, P234S, P234V, S235C, S235R, S235N, S235G, S235W, Y238R, Y238A, Y238Q, Y238C, Y238E, T240C, T240I, T240L, T240S, T243V, T243S, T243L, T243R, G244R, G244C, G244P, G244D, G244W, G245R, G245S, G245V, G245W, G245M, G246L, G246E, G246S, G246R, G246K, G246W, G246D, R247E, S248Y, S248P, S248V, S248L, S248F, S248A, S248E, S248W, S248K, S248T, A252E, A252T, A252Y, A252V, A252L, T254D, T254W, T254V, T254G, T254A, L255R, L255Q, L255P, L255G, Y262C, Y262Q, Y262S, Y262G, Y262V, Y262A, Y262W, S265C, S265P, S265G, S265L, G267W, G267C, A270L, A270M, A271W, A271Y, A271L, K279R, K279W, K279E, K279P, K279G, K279F, S282W, S282T, S282K, S282R, L284N, L284Q, L284T, L284S, L284R, L284G, L284V, V294G, V294W, V294E, V294S, Y295V, Y295R, S296F, S296L, S296W, S296K, I297S, I297P, I297K, I297F, I297R, I297W, N298W, N298G, N298C, N298V, N298L, N298A, S299P, S299C, S299M, S299L, S299T, G300S, G300A, G300P, G300L, G300W, A302G, A302L, A302C, A302R, A302V, S303P, S303V, S303C, S303A, S303R, N304T, N304R, N304Q, N304L, N304V, T309G, T309I, T309R, T309M, E314Y, E314T, E314V, E314G, E314S, E314L, E314A, S316T, S316L, S316G, S316F, S316R, S316P, S316V, S316Q, Q318L, Q318R, G319R, G319Q, G319P, G319A, T326V, T326G, T326W, T326N, T326A, V330S, V330L, V330P, V330R, V330A, V330G, N339P, N339A, N339T, E342M, E342W, E342N, E342L, E342R, S343R, S343C, E348W, E348F, E348P, E348V, E348G, E348M, S351P, S351C, S351G, S351R, S351L, S351W, T352P, T352L, T352G, T352Q, T352Y, Q359K, Q359P, Q359R, Q359S, Q359A, S362P, S262R, S262G, S262M, G363R, G363T, G363P, V364A, V364C, V364E, V364S, V364G, V364L, T365S, T365G, T365W, T365L, T365H, A366D, A366T, A366P, A366R, A366H, S371A, S371G, S372A, S372E, S372C, S372L, S372R, T378G, T378L, T378D, T378H, T378A, T378P, S381K, I383A, I383G, I383C, I383L, I383T, I383M, N385R, N385W, N385S, N385G, N385D, F386S, F386W, F386Q, F386V, F386I, F386G, F386C, F386A, F386T, F386L, A392V, A392L, A392E, A392G, N394D, N394R, N394Y, N394W, N394E, K396I, K396W, K396P, K396Y, K396F, Y408V, Y408E, Y408P, Y408S, Y408K, Y408L, K410S, K410R, D412M, D412S, D412N, D412W, D412L, D412R, S414C, S414R, S414G, S414V, S414W, S414H, S417Y, V419S, V419G, V419C, V419A, V419K, V419R, V419T, A426M, A426N, A426K, A426R, S427G, S427A, S427P, S427N, S427D, S427L, E433C, A434Q, A434G, N436S, N436P, N436D, N437K, N437R, N437T, N437P, T438E, T438G, Q439W, Q439S, Q439G, Q439C, Q439R, Q439Y, G442V, G442D, G442C, G442A, G442L, G442W, G442E, G442M, G442R, A446G, A446D, A446R, A446E, A446I, L448G, L448P, L448E, V450P, V450S, V450C, V450E, V450L, V450N, N470H, N470D, N470K, N470V, N470L, E472I, V474W, V474C, V474A, V474L, V474G, W475P, W475A, W475R, N478L, N478I, N478P, N478R, N478W, N478S, N478G, N478K, N478A, S484G, S484Y, S484P, S484A, S484N, V485A, V485W, V485K, V485G, V485R, D486I, D486K, D486Y, D486S, D486A, D486W, D486L, A487S, A487V, A487L, A487G, A487C, A487K, S492L, S492R, S492T, S492W, S492P, S492C, A493V, A493R, A493D, A493W, D494N, D494R, D494G, D494L, D494E, D494Q, N495L, N495W, N495G, N495R, N495C, S501R, S501L, S501M, S501K, S501W, A502C, A502Q, A502W, A502G, A502V, T506A, T506P, T506V, I509E, I509D, I509S, I509F, I509W, I509R, T510F, T510E, T510R, T510P, T510V, T510A, T510L, N512Q, N512K, N512H, N512R, N512V, S516R, S516W, S516P, S516K, S516Y, S516C, A518D, A518G, A518Y, A518V, A518R, A518L, A518T, I519W, I519L, I519R, I519F, I519K, N527T, N527K, N527P, N527L, N528D, N528G, N528K, N528V, N528E, N528L, A530R, A530C, A530G, A530V, A530S, A530T, E534W, E534Q, E534C, E534V, E534G, E534R, E534F, E534K, D536G, D536R, D536W, D536H, D536K, D536N, D536M, D536C, D536V, P537D, P537M, P537W, P537G, P537E, N538D, N538S, N538W, N538Y, N538A, N539M, N539R, N539P, N539A, I541A, I541T, I541V, I541G, I541N, A545R, A545T, A545V, A545L, S546P, S546G, S546C, S546E, S546N, G547D, G547S, G547V, S548P, S548W, S548L, S548G, S548T, N552V, N552E, N552F, N552A, N552R, N552G, and T554Q, T554G, wherein the positions correspond to amino acid positions in the amino acid sequence set forth in SEQ ID NO: 3; and wherein the substitution at the one or more positions provides a glucoamylase variant having an increase in melting temperature measured by TSA of at least 0.5 degrees Celsius, and further wherein the variants have at least at least 85%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 3.

Metadata:
- Claim Count in Document: 59.0
- Percentile: 91.0
- Lexical Diversity: 1.89394
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14787418', '14901953', '14426051', '14773673', '15112096']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6164486975782135
- 35 USC 102 Novelty (BERT): 0.5464997963585098
- Combined Prediction Score: 0.6094538074562431
- Mean Citation Score: 367.950342
- Max Citation Score: 422.01895
- Similarity Product: 258.09694680561427

Labels:
- Claim Label 101: 0
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test