PATENT CLAIM ANALYSIS

Application Number: 15944700
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-10
Patent Classification: ["514", "020800"]

Abstract:
Provided are non-naturally occurring cystine knot peptides (CKPs) that bind to VEGF-A. Additionally, provided are methods of using non-naturally occurring CKPs that bind to VEGF-A, including diagnostic and therapeutic compositions and methods. Non-naturally CKPs that bind low density lipoprotein receptor-related protein 6 (LRP6) are also provided.

Claim (Index 31):
The method of  claim 25 , wherein the CKP comprises the cystine scaffold structure:\n Z 1 C 1 L1C 2 L2C 3 L3C 4 L4C 5 L5C 6 Z 2  wherein:\n Z 1  and Z 2  are any amino acid, more than one amino acid, or an unnatural amino acid; \n L1 is Loop 1 and has a structure selected from the group consisting of: X 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 , X 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 , and X 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 X 10 , wherein each of X 1 -X 10  is any amino acid or an unnatural amino acid; \n L2 is Loop 2 and has the structure: X 1 X 2 X 3 X 4 X 5 , wherein each of X 1 -X 5  is any amino acid or an unnatural amino acid; \n L3 is Loop 3 and has the structure: X 1 X 2 X 3  wherein each of X 1 -X 3  is any amino acid or an unnatural amino acid; \n L4 is Loop 4 and has the structure: X 1 , wherein X 1  is any amino acid or an unnatural amino acid; L5 is Loop 5 and has the structure: X 1 X 2 X 3 X 4 X 5 , wherein each of X 1 -X 5  is any amino acid or an unnatural amino acid; \n wherein the unnatural amino acid selected from the group consisting of L-propargylglycine-PEG 6 -, L-sulfotyrosine, L-norleucine, L-1-naphthylalanine, L-2-naphthylalanine, L-2-chlorotryptophan, L-3-fluorotyrosine, L-4-fluorophenylalanine, gamma-benzyl-L-proline, gamma-(4-fluoro-benzyl)-L-proline, 4-OH-L-proline, 4-fluoro-L-proline, 4-[4-(trifluoromethyl)-benzyl]-L-proline, 3,4-difluoro-L-phenylalanine, 3,4-dichloro-L-phenylalanine, 4-chloro-L-phenylalanine, 3-F,4-Cl-L-phenylalanine, 2-pyridone(NH para)-L-alanine, pyridone(NH meta)-L-alanine, 3-(1-N-methyl indole)-L-alanine, 3-(1-N-ethyl indole)-L-alanine, 3-(1-N-isopropyl indole)-L-alanine, 3-(5-aza-indole)-L-alanine, 4-methyl-L-phenylalanine, 2-naphthyl-L-alanine, L-4,4\u2032-biphenylalanine, 3-(3-quinolinyl)-L-alanine, 3-(2-quinolinyl)-L-alanine, 3-(2-quinoxalinyl)-L-alanine, 4-methyl-2-pyridyl-alanine, 4-ethyl-2-pyridyl-L-alanine, benzothiazole-L-alanine, benzothiophene-L-alanine, 3-isoquinolinyl-L-alanine, t-butyl-L-alanine (also known as L-Nepentyl glycine), 3-cyclobutyl-L-alanine, cyclopentyl-L-alanine, 5,5,5-Trifluoro-L-leucine, t-butyl-L-glycine (also known as L-tert-Leucine), L-cyclopentylglycine, L-cyclobutylglycine, 3,4-hydroxy-L-phenylalanine, 3,4-fluoro-L-phenylalanine, 3-fluoro,4-OH-L-phenylalanine, 2-chloro-L-tyrosine, 2-methyl-L-tyrosine, 2-ethyl-L-tyrosine, 4-(naphthalen-1-ol)-L-alanine, D-serine, L-beta-homoserine, L-beta-alanine, N-alpha-methyl glycine, glycine amide, glycine ester of glycerol, glycine ester of glycol, glycine ester of oxetane-3-yl, and glycine morpholine amide, and \n wherein the CKP has an altered disulfide bond connectivity with reference to a wild-type  Ecballium elaterium  trypsin inhibitor EETI-II protein having the amino acid sequence set forth in SEQ ID NO: 1; wherein the altered disulfide bond connectivity is C1-C4, C2-C3 and C5-C6; and wherein the CKP has a percent alpha helix content of at least 20%.

Metadata:
- Claim Count in Document: 38.0
- Percentile: 91.0
- Lexical Diversity: 1.57143
- Patent Class: 514.0
- Transitional Phrase Type: closed
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15267087', '10182509', '14745562', '14384694', '14563362']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7708055948820207
- 35 USC 102 Novelty (BERT): 0.5862156746974244
- Combined Prediction Score: 0.7523466028635611
- Mean Citation Score: 328.71075600000006
- Max Citation Score: 560.7332
- Similarity Product: 371.6894338846208

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test