PATENT CLAIM ANALYSIS

Application Number: 15943320
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["424", "574000"]

Abstract:
A therapeutic serum suitable for inclusion in a cosmetic preparation may be produced by stressing a co-culture including proliferative cells. The co-culture of cells may be obtained by growing first culture to less than one-hundred percent confluence on a surface. After a monolayer of first culture is established, a second culture may be seeded onto at least one cell free area on the surface, the resulting co-culture grown to less than one-hundred percent confluence. Additional cultures may then be seeded onto cell free areas of the surface and established until a monolayer having the desired population of cells is obtained. The monolayer is then stressed to obtain a serum by conditioning a collection medium. The obtained serum may be combined with a suitable cosmetic base to provide a cosmetic preparation.

Claim (Index 1):
A method of producing a serum, comprising the steps of:\n seeding a first surface with a first culture of cells; growing the first culture to a monolayer of less than 100% confluence in the presence of a first growth medium as to provide at least one cell free area on the first surface, the first grown medium comprising nutrients and at least one growth factor, wherein the first growth medium promotes proliferation of the first culture; seeding a second culture onto the at least one cell free area of the first surface, the second culture comprising cells different from the first culture; growing the first and second culture to less than 100% confluence in the presence the second growth medium, the second growth medium comprising of nutrients and at least one growth factor, wherein the second growth medium promotes proliferation of the second culture; after growing the first and second cultures to less than 100% confluence, replacing the second growth medium with a collection medium, the collection medium lacking at least one of the growth factors of the second growth medium; maintaining the first and second cultures for a period of time in the presence of the collection medium to produce a conditioned medium; and collecting at least a portion of the conditioned medium after the period of time, said conditioned medium comprising a therapeutic serum.

Metadata:
- Claim Count in Document: 11.0
- Percentile: 91.0
- Lexical Diversity: 2.0
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15700895', '14597796', '13842129', '12349451', '10165267']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6755192431816031
- 35 USC 102 Novelty (BERT): 0.5689515737235609
- Combined Prediction Score: 0.6648624762357989
- Mean Citation Score: 263.360552
- Max Citation Score: 439.87875
- Similarity Product: 391.6315128743648

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test