PATENT CLAIM ANALYSIS

Application Number: 15877422
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-08
Patent Classification: ["435", "006100"]

Abstract:
cfDNA reference material, e.g., for use in verifying the accuracy and effectiveness of a diagnostic test, is generated from chromatin which may be sourced from whole cells. The chromatin may be treated with formaldehyde to form crosslinks between DNA and histones, e.g., while the chromatin is contained within nuclear and/or plasma membranes. The fixed chromatin may be sheared by acoustic energy, which may also be used to lyse cell membranes within which the fixed chromatin may be contained. The sheared chromatin may be treated with an enzyme, such as micrococcal nuclease, to digest chromatin in linker regions of the DNA between nucleosomes and generate nucleosome material for use as cfDNA reference material.

Claim (Index 17):
The method of  claim 1 , wherein the step of exposing the fixed chromatin includes arranging focused acoustic energy to have a duty cycle of 10% or less, and a peak incident power (PIP) of 20 W to 200 W.

Metadata:
- Claim Count in Document: 47.0
- Percentile: 86.0
- Lexical Diversity: 1.87879
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['13169815', '14784250', '15121667', '14362596', '14773826']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.601839082687078
- 35 USC 102 Novelty (BERT): 0.5095187781919364
- Combined Prediction Score: 0.5926070522375638
- Mean Citation Score: 205.776904
- Max Citation Score: 212.13203
- Similarity Product: 116.66490615569174

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test