PATENT CLAIM ANALYSIS

Application Number: 15754171
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["435", "377000"]

Abstract:
Provided is a preparation method for olfactory ensheathing cells. The method comprises the steps of formulation of a cell medium, collection and pretreatment of tissues, enzymatic digestion, cell culture, cryopreservation, and differentiation culture. The prepared olfactory ensheathing cells can maintain the proliferative capacity for a long time, and still possess the activity of olfactory ensheathing cells after 11 th  passage.

Claim (Index 19):
The method according to  claim 18 , wherein the preparation method further comprises a step of passage or cryopreservation of the olfactory ensheathing cells;\n preferably, during the passage, the culture supernatant of the cells to be passaged is collected, filtrated and mixed with a culture medium for olfactory ensheathing cells from olfactory mucosa in a ratio of 1:3 to formulate a culture medium for passage; preferably, the preparation method further comprises a step of cryopreservation of the olfactory ensheathing cells; preferably, the cryopreservation is cryopreservation at an ultralow temperature; preferably, the cryopreservation of the olfactory ensheathing cells at an ultralow temperature comprises the following steps: filtering the culture supernatant when the cells in step (2) grow adherently to 90% to prepare a cryopreservation medium for the cryopreservation of olfactory ensheathing cells; more preferably, the cryopreservation medium for olfactory ensheathing cells consists of the autologous serum, the culture supernatant, DMEM/F12 and DMSO; further preferably, the cryopreservation medium for olfactory ensheathing cells consists of the autologous serum, the culture supernatant, DMEM/F12 and DMSO in a ratio by volume of 5:2:2:1.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 88.0
- Lexical Diversity: 1.64286
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['12680604', '15166695', '11305829', '14233087', '13775556']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5673951715450852
- 35 USC 102 Novelty (BERT): 0.4937716582762699
- Combined Prediction Score: 0.5600328202182037
- Mean Citation Score: 198.39857
- Max Citation Score: 220.2932
- Similarity Product: 165.99852539689542

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test