PATENT CLAIM ANALYSIS

Application Number: 16328141
Application Type: Utility
Filing Date: 2019-02
Publication Date: 2019-06
Patent Classification: ["435", "006120"]

Abstract:
It is disclosed a method for amplification of nucleic acids in which substantially use is made of the fact that a pre-defined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target sequence-specific activator oligonucleotide. The target sequence-specific activator oligonucleotide causes the separation of re-synthesized complementary primer extension products by means of strand displacement, so that a new primer oligonucleotide can attach to the respective template strand. The thus formed complex of a primer oligonucleotide and a template strand can initiate a new primer extension reaction. The thus formed primer extension products in turn function as templates, so that an exponential amplification reaction results. Here, the activator oligonucleotide itself does not function as a template and preferably is inert over a primer extension reaction. The activator oligonucleotide functions as a co-factor in the reaction that regenerates the single-stranded state of the relevant strand segments of the templates needed for the reaction. By the use of a set of components comprising at least one primer oligonucleotide, at least one polymerase, a set of substrates for polymerase (e.g., dNTPs), and at least one specific activator oligonucleotide a nucleic acid to be amplified can be amplified linearly. By the use of such a set and a second primer oligonucleotide a nucleic acid to be amplified can be amplified exponentially, wherein the resulting specific primer extension products are used for further primer extension reactions as templates.

Claim (Index 10):
A method of utilizing a kit for carrying out the method according to  claim 1 , characterized in that the kit contains at least one first primer oligonucleotide, at least one activator oligonucleotide, and at least one polymerase for amplification.

Metadata:
- Claim Count in Document: 26.0
- Percentile: 99.0
- Lexical Diversity: 2.48544
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['12504485', '12681603', '11744553', '12185699', '11810834']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6786837593101422
- 35 USC 102 Novelty (BERT): 0.5508259693899438
- Combined Prediction Score: 0.6658979803181224
- Mean Citation Score: 407.16758
- Max Citation Score: 420.04858
- Similarity Product: 305.00106607884413

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test