PATENT CLAIM ANALYSIS

Application Number: 16006210
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-01
Patent Classification: ["424", "186100"]

Abstract:
Provided herein are methods for the purification of recombinant adeno-associated virus (rAAV) vectors that can be used for gene transfer and specifically for gene therapy or vaccination. Recombinant AAV vectors of the invention are substantially free of in-process impurities, including production components such as cellular nucleic acids, cellular proteins, helper virus, and media components.

Claim (Index 1):
A method for isolating a population of recombinant adeno-associated virus (rAAV) particles from in-process impurities in a feedstream, comprising the steps of:\n (a) (i) contacting a feedstream containing the rAAV particles with an apatite chromatography medium in the presence of polyethylene glycol (PEG), wherein the rAAV particles bind to the apatite chromatography medium; and\n (ii) eluting the rAAV particles bound to the apatite chromatography medium with an elution buffer containing less than 3% (w/v) PEG; and \n (b) (i) contacting the feedstream eluted from the apatite chromatography medium containing the rAAV particles with a hydrophobic interaction chromatography (HIC) medium in a high salt buffer comprising citrate, wherein the rAAV particles and the in-process impurities bind to the HIC medium; and\n (ii) eluting the rAAV particles bound to the HIC medium with a medium salt buffer.

Metadata:
- Claim Count in Document: 55.0
- Percentile: 94.0
- Lexical Diversity: 1.34783
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13328306', '10016767', '15350409', '10020482', '10851688']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7046296286089854
- 35 USC 102 Novelty (BERT): 0.6708959692295291
- Combined Prediction Score: 0.7012562626710398
- Mean Citation Score: 321.508458
- Max Citation Score: 662.2674599999998
- Similarity Product: 656.4615132593892

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test