PATENT CLAIM ANALYSIS

Application Number: 15886829
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-06
Patent Classification: ["435", "174000"]

Abstract:
The present invention relates to fields of continuous directional catalysis of soybean slurry to extract protein and functional oil with nano-magnetic immobilized enzymes. The present invention discloses soybean slurry as raw material and continuous mobile phase, and nano-magnetic immobilized enzymes as stationary phase applied in three-phase magnetic fluidized bed. The present invention provides a method of enzymatic disrupting cell walls by nano-magnetic immobilized enzyme cocktail including cellulose, pectinase and alkaline protease. The method can he used to break down lipoprotein, lipopolysaccharide complex and break lipoprotein film on the surface of lipoprotein to release the lipid. The present invention also provides a method to catalyze phospholipid using nano-magnetic immobilized phospholipase to change the polarity of phospholipids and control liquid emulsification phenomenon. The enzymes disclosed in the present invention do not interfere with each other. The preparation method can be used to catalyze soybean slurry continuously using the nano-magnetic immobilized enzymes to extract protein and functional lipid simultaneously.

Claim (Index 2):
The method for extracting proteins and functional lipid from soybean liquid by directional continuous catalysis with nano magnetic immobilized enzymes according to  claim 1 , wherein the method comprises following specific steps:\n heating the soybean slurry 50\u00b0 C., adding buffer and adjusting pH to 4.5; transferring the soybean slurry to a first tank of the five-order-three-phase fluidized bed reaction system with nano-immobilized magnetic cellulose dosage of 0.12 g/kg; reacting for 4.0 h for enzymolysis of cellulose in the soybean liquid and discharging the soybean liquid from the first tank; mixing the discharged soybean liquid with the buffer, adjusting pH value to 3.5, transferring to a mixer for mixing, heating to 55\u00b0 C. and transferring to a second tank of the five-order-three-phase fluidized bed reaction system with nano-immobilized pectinase dosage of 0.10 g/kg, reacting for 4.0 h for enzymolysis of pectinase in the soybean liquid and discharging the soybean liquid from the second tank; transferring soybean slurry to the third tank with adding nano-immobilized alkaline protease at the dosage of 0.10 g/kg, adjusting pH to 9.5, temperature of 55\u00b0 C., and reaction duration of 4.0 h; transferring soybean slurry to the fourth tank with adding nano-immobilized phospholipase C at the dosage of 0.80 g/kg, adjusting pH to 7.0, temperature of 60\u00b0 C., and reaction duration of 4.5 h to generate DAG from PC and PE; transferring soybean slurry to the fifth tank with adding nano-immobilized phospholipase A1 at the dosage of 0.40 g/kg, adjusting pH to 6.5, temperature of 60\u00b0 C., and reaction duration of 3.4 h to generate DAG from PC and PE; centrifuging to obtain lipid, skim and residue, with the protein yield of 94% (by mass of skim), the lipid yield of 96% (by mass of skim) and the content of DAG in lipid was 1.5%.

Metadata:
- Claim Count in Document: 13.0
- Percentile: 88.0
- Lexical Diversity: 1.90805
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15163795', '10592450', '11567318', '13502069', '13502105']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5868712929362784
- 35 USC 102 Novelty (BERT): 0.5077535983371517
- Combined Prediction Score: 0.5789595234763657
- Mean Citation Score: 192.021682
- Max Citation Score: 209.79166
- Similarity Product: 117.437363347286

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test