PATENT CLAIM ANALYSIS

Application Number: 16011460
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-04
Patent Classification: ["800", "008000"]

Abstract:
The invention provides methods for isolating cell-type specific mRNAs by selectively isolating ribosomes or proteins that bind mRNA in a cell type specific manner, and, thereby, the mRNA hound to the ribosomes or proteins that bind mRNA. Ribosomes, which are riboprotein complexes, bind mRNA that is being actively translated in cells. According to the methods of the invention, cells are engineered to express a molecularly tagged ribosomal protein or protein that binds mRNA by introducing into the cell a nucleic acid comprising a nucleotide sequence encoding a ribosomal protein or protein that binds mRNA fused to a nucleotide sequence encoding a peptide tag. The tagged ribosome or mRNA binding protein can then be isolated, along with the mRNA bound to the tagged ribosome or mRNA binding protein, and the mRNA isolated and further used for gene expression analysis. The methods of the invention facilitate the analysis and quantification of gene expression in the selected cell type present within a heterogeneous cell mixture, without the need to isolate the cells of that cell type as a preliminary step.

Claim (Index 77):
The non-human mammal of  claim 68 , wherein the mammalian endogenous promoter is from a characterizing gene and expression of the nucleic acid encoding the transactivator is substantially similar to expression of the characterizing gene.

Metadata:
- Claim Count in Document: 44.0
- Percentile: 94.0
- Lexical Diversity: 2.22093
- Patent Class: 800.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['13104316', '10494248', '14553937', '12049862', '14890705']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6323333957105022
- 35 USC 102 Novelty (BERT): 0.5814480517545061
- Combined Prediction Score: 0.6272448613149026
- Mean Citation Score: 358.377196
- Max Citation Score: 552.68445
- Similarity Product: 410.7687896115392

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test