PATENT CLAIM ANALYSIS

Application Number: 16145152
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-02
Patent Classification: ["435", "140000"]

Abstract:
The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.

Claim (Index 35):
A biosensor for measuring glucose in a sample liquid, comprising\n an electrode system and an enzyme reaction layer on an electrode of the electrode system, the enzyme reaction layer comprising an electron acceptor and flavin adenine dinucleotide (FAD)-linked glucose dehydrogenase (GLD) obtained from a transformed cell which comprises a nucleic acid encoding the GLD, wherein: (a) the GLD has an activity toward glucose of catalyzing dehydrogenation of glucose in the presence of the electron acceptor and has an activity toward maltose of 5% or less with respect to the activity toward glucose; (b) the GLD has an amino acid sequence comprising the sequences set forth in SEQ ID NOs. 8 to 12; and (c) the total content of galactose, glucose, mannose, and arabinose contained in the GLD is 10 \u03bcg or less per \u03bcg of protein.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 97.0
- Lexical Diversity: 1.92105
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15079002', '14184573', '15927949', '11886885', '15927976']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.603296978297526
- 35 USC 102 Novelty (BERT): 0.5784079091586958
- Combined Prediction Score: 0.600808071383643
- Mean Citation Score: 402.41279
- Max Citation Score: 412.19226
- Similarity Product: 402.7545374140906

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test