PATENT CLAIM ANALYSIS

Application Number: 16158276
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-03
Patent Classification: ["435", "006100"]

Abstract:
Provided herein is an internal standard method for determining copy number of a pathogen DNA in an unpurified nucleic acid sample by using a known copy number of synthetic DNA that shares a consensus region sequence with the pathogen. The sample is subject to two amplification steps using locus-specific primers and fluorescent primers respectively to obtain fluorescent amplicons for the pathogen and synthetic DNA. These are hybridized with immobilized pathogen-specific and synthetic DNA-specific nucleic acid probes and imaged to obtain fluorescent signals for pathogen-specific and synthetic DNA-specific amplicons. Signal intensities are correlated with the known copy number of synthetic DNA to determine copy number of pathogen DNA in the plant. Also described herein is a method to simultaneously quantitate using the above method, copy numbers of both pathogen and plant DNA in a sample.

Claim (Index 26):
The method of  claim 16 , wherein the pathogen is a fungus, the synthetic DNA has sequences shown in SEQ ID NO: 154 that hybridizes to synthetic DNA specific nucleic acid probes having sequences shown in SEQ ID NO: 145.

Metadata:
- Claim Count in Document: 52.0
- Percentile: 97.0
- Lexical Diversity: 2.08955
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['16158181', '15916036', '12100519', '09865807', '14694008']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6825810767105354
- 35 USC 102 Novelty (BERT): 0.6232350479869142
- Combined Prediction Score: 0.6766464738381732
- Mean Citation Score: 496.644404
- Max Citation Score: 717.77814
- Similarity Product: 488.5409706576776

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test