PATENT CLAIM ANALYSIS

Application Number: 16353636
Application Type: Utility
Filing Date: 2019-03
Publication Date: 2019-07
Patent Classification: ["435", "006100"]

Abstract:
The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

Claim (Index 18):
A method for measuring an amount of DNA in a biological sample, the method comprising:\n (a) performing a PCR and/or an allele-specific amplification for more than 100 SNP loci on one or more chromosomes expected to be disomic in a single reaction mixture using more than 100 primer pairs, wherein the reaction mixture comprises cell-free DNA extracted from a biological sample of a subject comprising DNA of mixed origin, wherein the DNA of mixed origin comprises DNA from the subject and DNA from a genetically distinct individual; (b) measuring a quantity of each allele at a plurality of amplified SNP loci that comprise an allele present in the genetically distinct individual but not the subject; (c) measuring an amount of the DNA from the genetically distinct individual in the biological sample using the quantity of each allele at the SNP loci and an expected quantity of each allele at the SNP loci for different DNA fractions.

Metadata:
- Claim Count in Document: 16.0
- Percentile: 99.0
- Lexical Diversity: 1.41463
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['16288022', '14918544', '15336630', '14538982', '14882763']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.692924795392662
- 35 USC 102 Novelty (BERT): 0.6242737079189514
- Combined Prediction Score: 0.686059686645291
- Mean Citation Score: 633.6698279999998
- Max Citation Score: 708.049
- Similarity Product: 521.4691590185165

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test