PATENT CLAIM ANALYSIS

Application Number: 15923954
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-07
Patent Classification: ["435", "006000"]

Abstract:
In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

Claim (Index 3):
The method of  claim 1 , further comprising:\n d) forming a second reaction mixture by combining:\n i) the nucleic acid sample; \n ii) a second allele-specific primer, wherein an allele-specific nucleotide portion of the second allele-specific primer is complementary to the second allelic variant of the target sequence; \n iii) a second allele-specific blocker probe that is complementary to a region of the target sequence comprising the first allelic variant, wherein said region encompasses a position corresponding to the binding position of the allele-specific nucleotide portion of the second allele-specific primer, and wherein the second allele-specific blocker probe comprises a minor groove binder; \n iv) a second locus-specific primer that is complementary to a region of the target sequence that is 3\u2032 from the second allelic variant and on the opposite strand; and \n v) a second detector probe; \n e) carrying out an amplification reaction on the second reaction mixture using the second allele-specific primer and the locus-specific primer, to form a second amplicon; and f) detecting the second amplicon by detecting a change in a detectable property of the detector probe, thereby detecting the second allelic variant of the target gene in the nucleic acid sample.

Metadata:
- Claim Count in Document: 283.0
- Percentile: 90.0
- Lexical Diversity: 1.69118
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12641321', '14445046', '14856448', '14182718', '14397405']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6405231751016122
- 35 USC 102 Novelty (BERT): 0.6486114064415263
- Combined Prediction Score: 0.6413319982356036
- Mean Citation Score: 518.833392
- Max Citation Score: 610.2238
- Similarity Product: 605.1521369645834

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test