PATENT CLAIM ANALYSIS

Application Number: 16246195
Application Type: Utility
Filing Date: 2019-01
Publication Date: 2019-05
Patent Classification: ["506", "016000"]

Abstract:
Multimeric barcoding reagents for labelling a target nucleic acid comprise: first and second barcode molecules linked together, wherein each of the barcode molecules comprises a nucleic acid sequence comprising a barcode region; and first and second barcoded oligonucleotides. The multimeric barcoding reagents enable spatial sequencing. A single multimeric barcoding reagent can be used to label sub-sequences of an intact nucleic acid molecule or co-localised fragments of a nucleic acid molecule. The labelled sub-sequences can be sequenced and the sequencing data processed to determine the sequence of sub-sequences from a single intact nucleic acid molecule or from co-localised fragments of a nucleic acid molecule. Corresponding libraries, kits, methods and uses are provided.

Claim (Index 1):
A method of generating a synthetic long read from a target nucleic acid comprising the steps of:\n a) preparing a nucleic acid sample for sequencing using a multimeric barcode molecule, wherein the multimeric barcode molecule comprises first, second, and third barcode molecules comprised within a nucleic acid molecule, wherein each of the barcode molecules comprises a nucleic acid sequence comprising a barcode region, and wherein preparing the nucleic acid sample for sequencing comprises producing first, second, and third barcoded target nucleic acid molecules, wherein the first barcoded target nucleic acid molecule comprises a first sub-sequence of the target nucleic acid and the nucleic acid sequence or complement thereof of the barcode region of the first barcode molecule, wherein the second barcoded target nucleic acid molecule comprises a second sub-sequence of the target nucleic acid and the nucleic acid sequence or complement thereof of the barcode region of the second barcode molecule, and wherein the third barcoded target nucleic acid molecule comprises a third sub-sequence of the target nucleic acid and the nucleic acid sequence or complement thereof of the barcode region of the third barcode molecule; b) sequencing the sample, wherein the step of sequencing comprises sequencing at least part of the first barcoded target nucleic acid molecule to generate a first sequence read, sequencing at least part of the second barcoded target nucleic acid molecule to generate a second sequence read, and sequencing at least part of the third barcoded target nucleic acid molecule to generate a third sequence read; and c) processing the sequence data obtained by step (b) to generate a synthetic long read from the target nucleic acid, wherein the synthetic long read comprises at least one nucleotide corresponding to the target nucleic acid from each of the first, second, and third sequence reads.

Metadata:
- Claim Count in Document: 13.0
- Percentile: 99.0
- Lexical Diversity: 1.89062
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15750824', '14947988', '15842687', '14006971', '14990276']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5808701909423445
- 35 USC 102 Novelty (BERT): 0.6312431544150189
- Combined Prediction Score: 0.585907487289612
- Mean Citation Score: 401.565432
- Max Citation Score: 631.3768
- Similarity Product: 540.5467940751075

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test