PATENT CLAIM ANALYSIS

Application Number: 16181261
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-02
Patent Classification: ["435", "325000"]

Abstract:
The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

Claim (Index 32):
A method for dedifferentiating or reprogramming feeder-free, xeno-free and integration-free mammalian somatic cells to produce feeder-free, xeno-free and integration-free induced pluripotent stem cells comprising:\n a) seeding mammalian somatic cells in a volume of 0.5 ml to 1 ml of xeno-free culture medium on a feeder-free and xeno-free surface; b) plating the mammalian somatic cells from step a) at a cell density of 15,000 cells/ml to 500,000 cells/ml; c) transfecting the plated mammalian somatic cells from step b) with a composition comprising:\n 1) one ml or less of xeno-free culture medium: \n 2) an effective amount of a fusion product between a synthetic mRNA encoding OCT4 and an N-terminal-linked transactivation domain, and \n 3) size-exclusion chromatography purified synthetic mRNA reprogramming factors encoding SOX2 KLF4, NANOG, and optionally c-MYC or LIN28, wherein the synthetic mRNA reprogramming factors are provided at a concentration of up to about 800 ng/ml. \n d) culturing said somatic cells with the composition from step c) under feeder-free and xeno-free culture conditions until the media is changed once daily; and e) repeating step d) until the mammalian somatic cells are reprogrammed or de-differentiated to produce feeder-free, xeno-free and integration-free induced pluripotent stem cells.

Metadata:
- Claim Count in Document: 83.0
- Percentile: 98.0
- Lexical Diversity: 1.58571
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14292317', '13893166', '15148230', '12962498', '14322526']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5854793678389227
- 35 USC 102 Novelty (BERT): 0.6595289394711574
- Combined Prediction Score: 0.5928843250021463
- Mean Citation Score: 465.348888
- Max Citation Score: 633.7559
- Similarity Product: 624.6555362909735

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test