PATENT CLAIM ANALYSIS

Application Number: 16017157
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["800", "023000"]

Abstract:
The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.

Claim (Index 15):
A method for producing a non-human mammal from a somatic cell nuclear transfer (SCNT) embryo, the method comprising:\n transferring a nucleus of a non-human mammalian cell to an enucleated non-human mammalian oocyte and culturing the oocyte to form a non-human mammalian SCNT embryo, contacting at least one of the non-human mammalian cell, the mammalian oocyte, or one or more cells of the non-human somatic mammalian SCNT embryo with an agent that decreases the expression or activity of an H3K9 methyltransferase in the non-human mammalian cell, the mammalian oocyte, or one or more cells of the non-human somatic mammalian SCNT embryo, implanting the non-human mammalian SCNT embryo into the oviduct of a non-human mammalian female surrogate; and allowing the non-human mammalian SCNT embryo to develop into a non-human mammal offspring.

Metadata:
- Claim Count in Document: 32.0
- Percentile: 94.0
- Lexical Diversity: 1.80597
- Patent Class: 800.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14276810', '12122557', '12528212', '14775594', '10865369']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.595195824684555
- 35 USC 102 Novelty (BERT): 0.5485228218083981
- Combined Prediction Score: 0.5905285243969394
- Mean Citation Score: 354.94936399999995
- Max Citation Score: 431.14233
- Similarity Product: 331.57630582200227

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test