PATENT CLAIM ANALYSIS

Application Number: 16067319
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-01
Patent Classification: ["435", "006110"]

Abstract:
The invention relates to a method and means for diagnosing tumors, in particular for an early diagnosis (prevention) and for differentiating between benign and malignant tumors using a PCR, in particular in bodily fluids. The method according to the invention is characterized by a combination of a pre-amplification process using a PCR, wherein methylated DNA sequences are amplified more strongly than non-methylated DNA sequences, and a subsequent quantification process using a special digital PCR, in which significantly more DNA is used than normally in the prior art. As comparison data indicates, the invention advantageously allows a clear reliable conclusion as to whether a malignant tumor disease is present or not. The invention is suitable for screening (prevention), monitoring the progress of a tumor disease, in particular to order to exclude a minimal residual disease (MRD), and for a differential diagnosis between malignant carcinoma and benign tumors.

Claim (Index 9):
Kit for diagnosing a tumour disease in an isolated sample, comprising:\n i) primers for pre-amplifying methylated and unmethylated DNA sequences in the genes PLA2R1, RASSF1A and GSTP1 by means of PCR, each primer being selected such that forward and reverse primers comprise from zero to seven 5\u2032-CG-3\u2032 dinucleotide sequences per primer pair, preferably from two to six, preferably from three to five, particularly preferably from one to at most three, more preferably either one or two 5\u2032-CG-3\u2032 dinucleotide sequences per primer pair, ii) probes for quantifying the methylated and unmethylated DNA sequences in the genes PLA2R1, RASSF1A and GSTP1.

Metadata:
- Claim Count in Document: 5.0
- Percentile: 94.0
- Lexical Diversity: 1.94118
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14079649', '12159081', '11884406', '13224024', '12219378']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6501518722580651
- 35 USC 102 Novelty (BERT): 0.5306628633223718
- Combined Prediction Score: 0.6382029713644959
- Mean Citation Score: 361.254908
- Max Citation Score: 377.0466
- Similarity Product: 237.59064125089645

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test