PATENT CLAIM ANALYSIS

Application Number: 15873022
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-05
Patent Classification: ["435", "007200"]

Abstract:
The invention provides methods for identifying immunobinders, such as scFv antibodies, capable of specifically binding to cell surface antigens, and compositions identified according to said methods.

Claim (Index 1):
A humanized immunobinder capable of binding to an antigen of interest, wherein the immunobinder comprises:\n a variable heavy chain comprising three heavy chain complementarity determining regions (CDRs) and four framework region sequences having at least 90% identity to the four framework region sequences of SEQ ID NO: 4, wherein the framework region sequences have one or more amino acid residues selected from the group consisting of: Threonine (T) at position 24, Valine (V) at position 25, Glycine or Alanine (G/A) at position 56, Lysine (K) at position 82, Threonine (T) at position 84, Valine (V) at position 89 and Arginine (R) at position 108 (AHo numbering), and a variable light chain comprising three light chain CDRs and a light chain framework sequence, wherein the heavy chain and light chain CDRs are from an immunobinder derived from a B-cell clone identified by:\n (a) providing a plurality of B-cells; \n (b) contacting the plurality of B-cells with the antigen of interest, wherein the antigen of interest comprises a first sortable label, and staining the plurality of B-cells with anti-IgG antibodies comprising a second sortable label and anti-IgM antibodies comprising a third sortable label; \n (c) separating from the plurality of B-cells one or more B-cells that can specifically bind to the antigen of interest using a cell sorter, wherein the presence of the first and second sortable label, but not the third sortable label, in a complex is indicative of the binding of a B-cell to an antigen of interest, thereby identifying a B-cell clone that binds to the antigen of interest.

Metadata:
- Claim Count in Document: 44.0
- Percentile: 86.0
- Lexical Diversity: 1.15385
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14947133', '14273231', '12710579', '13616267', '14965488']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6601088544534083
- 35 USC 102 Novelty (BERT): 0.6598571302628808
- Combined Prediction Score: 0.6600836820343555
- Mean Citation Score: 546.8980339999998
- Max Citation Score: 636.46155
- Similarity Product: 631.3201131006987

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test