PATENT CLAIM ANALYSIS

Application Number: 16061574
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["435", "221000"]

Abstract:
Provided is a mutant of the wild type phosphatidylcholine-specific phospholipase C of  Bacillus cereus . The mutations involved comprise the amino acid residue at position 63 being mutated from asparagine to aspartic acid, the amino acid residue at position 131 being mutated from asparagine to serine, and the amino acid residue at position 134 being mutated from asparagine to aspartic acid, and may comprise the amino acid residue at position 56 being mutated from tyrosine to alanine, lysine, asparagine, glutamine, histidine or tryptophan, and further, may also comprise the amino acid residue at position 106 being mutated from methionine to valine. Also provided are a polynucleotide molecule encoding the mutant, a nucleic acid construct and a host cell comprising the polynucleotide molecule, a composition comprising the mutant, and the use of the mutant, the polynucleotide molecule, the nucleic acid construct and the host cell.

Claim (Index 10):
The method according to  claim 9 , wherein the method has one or more of the following features:\n (1) the phospholipase C has an amino acid sequence comprising:\n (a) an amino acid sequence as set forth in SEQ ID NO: 7; or \n (b) a polypeptide derived from the amino acid of (a) by substitution, deletion or addition of one or several amino acids in the amino acid sequence in (a) while retaining the phospholipase C activity of SEQ ID NO: 7; \n (2) the phospholipase C is incubated at a temperature between 60\u00b0 C. and 70\u00b0 C.; (3) the incubation time is 15 to 45 minutes; (4) the enzyme is added at an amount of 50 to 1000 ppm based on the weight of crude oil; (5) the crude oil is heated to 50 to 70\u00b0 C. prior to adding the enzyme to crude oil; and (6) the degumming comprises stirring at 50 to 60\u00b0 C. for 1 to 3 hours, then heating to 80 to 90\u00b0 C. and holding for 1 to 10 minutes.

Metadata:
- Claim Count in Document: 14.0
- Percentile: 94.0
- Lexical Diversity: 2.58065
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15035105', '14902448', '10592450', '13502069', '15521654']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5714024124412153
- 35 USC 102 Novelty (BERT): 0.5451495189919331
- Combined Prediction Score: 0.568777123096287
- Mean Citation Score: 302.533364
- Max Citation Score: 399.3739
- Similarity Product: 285.3127829455972

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test