PATENT CLAIM ANALYSIS

Application Number: 15751792
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-10
Patent Classification: ["530", "388100"]

Abstract:
A method for separating cells capable of producing target antigen-specific monoclonal antibodies (TASMAs) wherein a cell group including antibody-producing cells is immobilized using a reversible crosslinking agent having cell membrane-permeating properties. The immobilized cell group is subjected to cell membrane dissolution using a surface active agent; and the cell group is reacted with a labeling target antigen. In the stained cell group a that has reacted with the labeling target antigen is separated. A method to produce TASMAs by separating mRNA from the cell separated using the method; preparing cDNA and preparing antigen-specific monoclonal antibodies or fragments thereof from the prepared cDNA. Also provided are a method whereby at least one cell capable of producing TASMAs is separated and a method whereby said antibodies can be produced by using the separated cell. Threonine 18 phosphorylated p53 (pT18-p53) and threonine 68 phosphorylated CHK2 (pT68-CHK2) specific monoclonal antibodies are also disclosed.

Claim (Index 1):
A method for isolating a cell capable of producing a monoclonal antibody with specificity toward an antigen of interest, wherein the method comprises the following steps;\n (1) fixing a cell population containing antibody-producing cells with a crosslinking reagent, wherein the crosslinking reagent is a reversible crosslinking regent having the cell membrane permeability (a fixing step); (2) treating the fixed cell population with a detergent (a cell membrane lysis step); (3) reacting the cell membrane lysed cell population with a labelled antigen of interest (a staining step); and (4) isolating at least one single cell which has been reacted with the labelled antigen of interest from the cell population that underwent the staining step, wherein the cell membrane lysis step (2) and the staining step (3) can be carried out sequentially or in parallel.

Metadata:
- Claim Count in Document: 27.0
- Percentile: 88.0
- Lexical Diversity: 1.96386
- Patent Class: 530.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['13425538', '15042106', '14787478', '10338366', '15361936']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6605869973260533
- 35 USC 102 Novelty (BERT): 0.5157866598425747
- Combined Prediction Score: 0.6461069635777054
- Mean Citation Score: 284.8170320000001
- Max Citation Score: 292.74838
- Similarity Product: 220.7239925405669

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test