PATENT CLAIM ANALYSIS

Application Number: 15907470
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-09
Patent Classification: ["435", "440000"]

Abstract:
The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GOI genome target location and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts from that point on.

Claim (Index 14):
A method for mammalian cell line development comprising the following steps:\n (a) providing a cell or cell population from a mammalian cell bank developed according to  claim 1  and having a final template DNA construct with two recombinase recognition sequences flanking a template protein of interest coding region and a first selection marker coding region in any order and orientation and wherein promoters for said coding regions can either be flanked by or be located outside of said recombinase recognition sequences and wherein said recombinase recognition sequences are both of the same type such as a serine recombinase type such as attP/attB or a tyrosine recombinase type such as Lox, Rox or FRT; (b) providing a matching expression vector in the form of a plasmid containing two recombinase recognition sequences flanking a desired protein of interest coding region and a second selection marker coding region, and wherein said recombinase recognition sequences in the expression vector are of the same type as and matching said recombinase recognition sequences in said template DNA construct; (c) introducing said expression vector together with vector(s) coding for a recombinase, and wherein said recombinase is of any type matching said recombinase recognition sequences in said template DNA construct and said expression vector such as one selected from the group of PhiC31, Cre, Dre, or Flp; (d) said recombinase catalyzes the exchange of the regions flanked by said recognition sequences in said final template DNA construct and said expression vector; (e) a cell or cells having undergone correct cassette exchange only are selected via the use of selection marker(s) and/or genetic characterization.

Metadata:
- Claim Count in Document: 24.0
- Percentile: 88.0
- Lexical Diversity: 1.38462
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14967689', '14092786', '13752647', '12793898', '12507252']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5852887898663645
- 35 USC 102 Novelty (BERT): 0.5184001661422488
- Combined Prediction Score: 0.578599927493953
- Mean Citation Score: 308.898376
- Max Citation Score: 313.97794
- Similarity Product: 242.14455992587563

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test