PATENT CLAIM ANALYSIS

Application Number: 15741883
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2019-01
Patent Classification: ["204", "548000"]

Abstract:
The invention provides a capillary isoelectric focusing (cIEF) system based on a sandwich injection method for automated chemical mobilization. This system was coupled with an electrokinetically pumped nanoelectrospray interface to a mass spectrometer. The nanoelectrospray emitter employed an acidic sheath electrolyte. To realize automated focusing and mobilization, a plug of ammonium hydroxide was first injected into the capillary, followed by a section of mixed sample and ampholyte. As focusing progressed, the NH 3 H 2 O section was titrated to lower pH buffer by the acidic sheath buffer. Chemical mobilization started automatically once the ammonium hydroxide was consumed by the acidic sheath flow electrolyte.

Claim (Index 1):
A method for one-step capillary isoelectric focusing (cIEF) and chemical mobilization, the method comprising:\n (a) performing a separation on a separation capillary having an anode end and a cathode end, wherein the cathode end of the separation capillary is interfaced to an electrokinetically pumped nanoelectrospray emitter on a mass spectrometer; (b) partially filling the anode end of the separation capillary, first, with a length of a catholyte solution, then second, with a mixture comprising an ampholyte and analytes; (c) feeding the nanoelectrospray emitter with an acidic sheath electrolyte solution and an anolyte solution, wherein the anolyte solution feeds into the nanoelectrospray emitter by flowing out from the cathode end of the separation capillary; (d) applying a voltage to the anode and cathode ends of the separation capillary; (e) inducing isoelectric focusing of the analytes and concomitant chemical mobilization of the analytes toward the cathode end of the separation capillary; and (f) forming a nanoelectrospray, wherein the acidic sheath electrolyte solution ensheaths the mixture flowing out the cathode end of the separation capillary; wherein a pH gradient is established by the catholyte solution and the anolyte solution to induce isoelectric focusing of the analytes, and as the catholyte solution is titrated to lower pH by the acidic sheath electrolyte solution the analytes are chemically mobilized, then electrokinetically pumped toward the mass spectrometer by the acidic sheath electrolyte solution, thereby forming the nanoelectrospray when the voltage is applied.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 86.0
- Lexical Diversity: 1.57534
- Patent Class: 204.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14395862', '13573799', '12094969', '09832807', '12358724']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.8049938238353748
- 35 USC 102 Novelty (BERT): 0.5416544037417877
- Combined Prediction Score: 0.7786598818260161
- Mean Citation Score: 310.21396
- Max Citation Score: 414.71664
- Similarity Product: 320.829234774456

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test