PATENT CLAIM ANALYSIS

Application Number: 16056310
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-12
Patent Classification: ["435", "440000"]

Abstract:
Described herein are methods and vectors for rational, multiplexed manipulation of chromosomes within open reading frames (e.g., in protein libraries) or any segment of a chromosome in a cell or population of cells, in which various CRISPR systems are used.

Claim (Index 1):
A method for tracking a multitude of genome edits in a cell population, comprising:\n a) introducing into a first population of cells at least one first vector that encodes: (i) at least one editing cassette wherein the editing cassette comprises a) a region homologous to a target region of a nucleic acid and comprising a mutation of at least one nucleotide relative to the target region, and b) a mutation preventing nuclease binding at a PAM or spacer; (ii) at least one promoter; and (iii) a region encoding at least one guide RNA comprising a region complementary to a portion of the target region, thereby producing a second population of cells that comprise the at least one first vector; b) maintaining the second population of cells under conditions in which a nuclease is expressed thereby producing a cell library that does not comprise the mutation preventing nuclease binding at the PAM or spacer; and c) tracking the multitude of genome edits in the cell library by sequencing the editing cassette of the first vector from at least two cells in the cell library or sequencing the target region of at least two cells in the cell library.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 96.0
- Lexical Diversity: 1.24324
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15948785', '15116616', '15630909', '15948789', '15948798']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.776330769904248
- 35 USC 102 Novelty (BERT): 0.6711929096657235
- Combined Prediction Score: 0.7658169838803955
- Mean Citation Score: 682.8296280000002
- Max Citation Score: 752.7857700000003
- Similarity Product: 640.0487915168436

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test