PATENT CLAIM ANALYSIS

Application Number: 15949457
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-10
Patent Classification: ["424", "422000"]

Abstract:
Described herein are preparations and applications of gellan gum hydrogel for cell culture and biomedical applications, including 2D coating culture, 3D cell culture, and injection. The gellan gum materials for such application include water soluble low acyl gellan gum, high acyl gellan gum, modified gellan gum and a mixture of gellan gum mixture with other chemical/biological molecules.

Claim (Index 36):
The use according to  claim 34 , wherein a two-step procedure is provided for in vitro 3D cell culture in the hydrogel, comprising:\n 1) living cells being mixed with gellan gum solution or trigger solution such as cell culture media before the hydrogel formation, wherein the cells are homogeneously suspended in the soft hydrogel and ready to transfer to an individual cell culture plate or different container by pipetting or injection; and 2) once the soft gel is placed in the final container, where the extra trigger solutions are added on the top or surround the soft gel and convert it into the hard hydrogel, wherein the 3D matrix structure is further stabilized and nutrition or other biomolecules are added into the hydrogel system and exchange between the hydrogel and surrounding media.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 91.0
- Lexical Diversity: 1.68421
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14782812', '13637588', '15778380', '15746193', '11611570']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6822562435965619
- 35 USC 102 Novelty (BERT): 0.5336406085342448
- Combined Prediction Score: 0.6673946800903302
- Mean Citation Score: 250.40783
- Max Citation Score: 340.82526
- Similarity Product: 227.5933810067153

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test