PATENT CLAIM ANALYSIS

Application Number: 15893104
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["435", "475000"]

Abstract:
The present disclosure provides methods and kits for generating recombinant bacteriophage genomes. Specifically, the present technology provides methods of integrating a heterologous nucleic acid sequence into a bacteriophage DNA genome, and isolating recombinant bacteriophages that express the heterologous nucleic acid sequence.

Claim (Index 1):
A method for making a recombinant bacteriophage DNA genome in a first bacterial host cell comprising\n (a) contacting a first bacteriophage DNA genome with a first sgRNA-CRISPR enzyme complex and a second sgRNA-CRISPR enzyme complex in vivo under conditions where\n (i) the first sgRNA-CRISPR enzyme complex cleaves a first protospacer sequence within the first bacteriophage DNA genome; and \n (ii) the second sgRNA-CRISPR enzyme complex cleaves a second protospacer sequence within the first bacteriophage DNA genome to produce a cleaved first bacteriophage DNA genome; and \n (b) recombining in vivo the cleaved first bacteriophage DNA genome with a heterologous nucleic acid sequence in the presence of a recombination system under conditions to produce the recombinant bacteriophage DNA genome, wherein the first bacterial host cell is infected with the first bacteriophage DNA genome, and wherein the heterologous nucleic acid sequence comprises an open reading frame that encodes a bioluminescent protein, a fluorescent protein, a chemiluminescent protein, or any combination thereof.

Metadata:
- Claim Count in Document: 77.0
- Percentile: 88.0
- Lexical Diversity: 1.45161
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15893094', '15179912', '14293498', '15420936', '14523799']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5719434110277644
- 35 USC 102 Novelty (BERT): 0.6224462146437987
- Combined Prediction Score: 0.5769936913893678
- Mean Citation Score: 468.44034800000014
- Max Citation Score: 692.9609
- Similarity Product: 506.0424216755689

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test