PATENT CLAIM ANALYSIS

Application Number: 16060404
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-01
Patent Classification: ["424", "204100"]

Abstract:
A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates

Claim (Index 22):
A method for separating recombinant AAVrh10 viral particles containing DNA comprising pharmacologically active genomic sequences from genome-deficient(empty) AAVrh10 capsid intermediates, said method comprising:\n (a) forming a loading suspension comprising:\n recombinant AAVrh10 viral particles and empty AAV rh10 capsid intermediates which have been purified to remove non-AAV materials from an AAV producer cell culture in which the particles and intermediates were generated; and a Buffer A comprising 20 mM Bis-Tris propane (BTP) and a pH of about 10.0; \n (b) loading the suspension of (a) onto a strong anion exchange resin, said resin being in a vessel having an inlet for flow of a suspension and/or solution and an outlet permitting flow of eluate from the vessel; (c) washing the loaded anion exchange resin with Buffer 1% B which comprises 10 mM NaCl and 20 mM BTP with a pH of about 10.0; (d) applying an increasing salt concentration gradient to the loaded and washed anion exchange resin, wherein the salt gradient ranges from 10 mM to about 190 mM NaCl, inclusive of the endpoints, or an equivalent; and (e) collecting the rAAV particles from eluate, said rAAV particles being purified away from rh10 intermediates.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 94.0
- Lexical Diversity: 1.26923
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15544498', '13328306', '15081001', '10166347', '10016767']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7372025679714637
- 35 USC 102 Novelty (BERT): 0.5312105340260529
- Combined Prediction Score: 0.7166033645769226
- Mean Citation Score: 331.86248
- Max Citation Score: 367.2632
- Similarity Product: 274.86581073646545

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test