PATENT CLAIM ANALYSIS

Application Number: 15887894
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2019-01
Patent Classification: ["530", "387100"]

Abstract:
Herein is reported a method for purifying cell cultivation supernatants either directly after fermentation or after one or more preliminary purification steps, such as protein A affinity chromatography. By adjusting the pH value in the acid range and subsequent incubation of the acidified solution host cell nucleic acid and host cell protein can be precipitated but the target polypeptide remains in solution. Thereafter the precipitate and therewith the contaminating host cell components can be removed by a simple physical separation step.

Claim (Index 1):
A method for producing an immunoglobulin comprising the following steps:\n i) adding a solution consisting of an acid and water to a cell cultivation supernatant from which cells and cell debris have been removed for adjusting the pH value to a value of from pH 4.5 to pH 5.5, whereby the solution is essentially free of divalent cations, ii) incubating the pH adjusted cell cultivation supernatant, and iii) removing the precipitate from the incubated cell cultivation supernatant and thereby producing the immunoglobulin, wherein the cell cultivation supernatant comprises the immunoglobulin at a concentration of not more than 10 mg/ml, wherein the concentration of the added acid is 5.5 mol/l or lower.

Metadata:
- Claim Count in Document: 12.0
- Percentile: 88.0
- Lexical Diversity: 1.37097
- Patent Class: 530.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['13651188', '15216099', '13883243', '15029682', '13261193']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6186489952656444
- 35 USC 102 Novelty (BERT): 0.4890999061766626
- Combined Prediction Score: 0.6056940863567464
- Mean Citation Score: 219.43621800000005
- Max Citation Score: 232.27753
- Similarity Product: 190.04039102785947

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test