PATENT CLAIM ANALYSIS

Application Number: 15746781
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2019-01
Patent Classification: ["506", "016000"]

Abstract:
Methods of preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample are provided. Aspects of the methods include combining the RNA sample with a first strand cDNA primer and a template switch oligo-nucleotide under first strand cDNA synthesis conditions, where one of the first strand cDNA primer and the template switch oligo-nucleotide includes a first post-tagmentation amplification primer binding domain. The resultant product is subjected to amplification conditions sufficient to produce a double stranded cDNA, which is then tagmented with a transposome that includes a second post-tagmentation amplification primer binding domain. The tagmented sample is then subjected to amplification conditions using first and second post-tagmentation amplification primers that include sequencing platform adapter constructs to produce a NGS library. Aspects of the invention further include compositions produced by the methods and kits that find use in practicing the methods.

Claim (Index 16):
A composition comprising a tagmented sample produced by tagmenting a double stranded cDNA according to  claim 15  with a transposome comprising a transposase and a transposon nucleic acid comprising a transposon end domain and a second post-tagmentation amplification primer binding domain to produce a tagmented sample.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 86.0
- Lexical Diversity: 2.04
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14148463', '15668321', '14841981', '12605337', '14566445']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.597141830546704
- 35 USC 102 Novelty (BERT): 0.5061755842309664
- Combined Prediction Score: 0.5880452059151303
- Mean Citation Score: 269.57804000000004
- Max Citation Score: 286.6797
- Similarity Product: 220.9408601829887

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test