PATENT CLAIM ANALYSIS

Application Number: 16328141
Application Type: Utility
Filing Date: 2019-02
Publication Date: 2019-06
Patent Classification: ["435", "006120"]

Abstract:
It is disclosed a method for amplification of nucleic acids in which substantially use is made of the fact that a pre-defined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target sequence-specific activator oligonucleotide. The target sequence-specific activator oligonucleotide causes the separation of re-synthesized complementary primer extension products by means of strand displacement, so that a new primer oligonucleotide can attach to the respective template strand. The thus formed complex of a primer oligonucleotide and a template strand can initiate a new primer extension reaction. The thus formed primer extension products in turn function as templates, so that an exponential amplification reaction results. Here, the activator oligonucleotide itself does not function as a template and preferably is inert over a primer extension reaction. The activator oligonucleotide functions as a co-factor in the reaction that regenerates the single-stranded state of the relevant strand segments of the templates needed for the reaction. By the use of a set of components comprising at least one primer oligonucleotide, at least one polymerase, a set of substrates for polymerase (e.g., dNTPs), and at least one specific activator oligonucleotide a nucleic acid to be amplified can be amplified linearly. By the use of such a set and a second primer oligonucleotide a nucleic acid to be amplified can be amplified exponentially, wherein the resulting specific primer extension products are used for further primer extension reactions as templates.

Claim (Index 1):
A method for amplification of a nucleic acid comprising the following steps:\n a) hybridizing a first primer oligonucleotide to the 3\u2032 segment of a strand of a nucleic acid chain to be amplified, wherein the nucleic acid chain to be amplified comprises a target sequence, wherein the first primer oligonucleotide comprises:\n a first primer region in the 3\u2032 segment of the first primer oligonucleotide that can sequence-specifically bind to a strand of a nucleic acid chain to be amplified, \n a second region that is directly or via a linker linked to the 5\u2032 end of the first primer region of the first primer oligonucleotide and that comprises a polynucleotide tail which is suitable for binding an activator oligonucleotide and supporting strand displacement (step c) by the activator oligonucleotide, wherein the polynucleotide tail remains substantially uncopied by polymerase under the selected reaction conditions, \n b) extending the first primer oligonucleotide by means of a polymerase to form a first primer extension product comprising a sequence that is complementary to the target sequence of the nucleic acid chain (a) to be amplified, c) binding the activator oligonucleotide to the polynucleotide tail of the second region of the first extended primer oligonucleotide, wherein the activator oligonucleotide comprises:\n a first single-stranded region that can bind to the polynucleotide tail of the second region of the first primer oligonucleotide, \n a second single-stranded region that is substantially complementary and can bind to the first region of the first primer oligonucleotide, \n a third single-stranded region that is substantially complementary to at least a segment of the extension product, which has been synthesized by polymerase, of the first primer extension product, \n wherein the activator oligonucleotide does not serve as template for a primer extension of the first primer oligonucleotide, d) binding the activator oligonucleotide to the first primer region of the first extended primer oligonucleotide by displacing the strand of the nucleic acid chain to be amplified that is complementary to said first primer region, e) binding the activator oligonucleotide to the complementary segment of the extension product of the first extended primer oligonucleotide by displacing the strand of the nucleic acid chain to be amplified that is complementary to said extension product, wherein the 3\u2032 segment of the first primer extension product becomes single-stranded, f) hybridizing a second oligonucleotide primer to the first primer extension product, wherein the 3\u2032 segment of the second oligonucleotide primer comprises a sequence that can hybridize to the first primer extension product; and g) extending the second oligonucleotide primer with polymerase to form a second primer extension product, wherein the extension takes place up to and including the first primer region of the first primer oligonucleotide and said first primer region is copied by the polymerase, wherein the polynucleotide tail of the second region remains uncopied, h) repeating steps a)-g) until the desired degree of amplification has been achieved.

Metadata:
- Claim Count in Document: 26.0
- Percentile: 99.0
- Lexical Diversity: 2.48544
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['12504485', '12681603', '11744553', '12185699', '11810834']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6506599941140802
- 35 USC 102 Novelty (BERT): 0.5514606448639315
- Combined Prediction Score: 0.6407400591890653
- Mean Citation Score: 407.16758
- Max Citation Score: 420.04858
- Similarity Product: 338.015052330513

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test