PATENT CLAIM ANALYSIS

Application Number: 15755721
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-11
Patent Classification: ["435", "007320"]

Abstract:
The invention relates generally to immunoassays, and more particularly to monoclonal antibodies and immunoassays for the differential detection and quantitation of a wild-type crystal protein, such as a wild-type-Cry1Ab, from  Bacillus thuringiensis  and hybrid crystal proteins, which comprise all or a significant portion of the wild-type Cry protein in complex biological samples comprising both the wild-type Cry protein and one or more of the hybrid Cry proteins.

Claim (Index 70):
An immunoassay method to detect or quantitate a Cry1Ab protein and a hybrid Cry protein having at a contiguous 27% of the Cry1Ab protein's amino acid sequence in a biological sample comprising the Cry1Ab protein and the hybrid Cry protein, the method comprising: (a) coating a first solid surface with a first antibody that binds the Cry1Ab protein but does not bind the hybrid Cry protein and coating a second solid surface with a second antibody that binds the hybrid Cry protein; (b) contacting the biological sample with the first antibody under conditions effective to allow the formation of a Cry1Ab-first antibody complex but does not allow the formation of a hybrid Cry protein-first antibody complex, resulting in a Cry1Ab-depleted biological sample; (c) removing the Cry1Ab-depleted biological sample and contacting the Cry1Ab-depleted biological sample with the second antibody under conditions effective to allow the formation of a hybrid Cry protein-second antibody complex; (d) detecting or quantitating the Cry1Ab protein complex on the first solid surface; and (e) detecting or quantitating the hybrid Cry protein on the second solid surface.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 88.0
- Lexical Diversity: 1.6
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['11372065', '13623921', '10739482', '12529246', '13669130']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.592716022541503
- 35 USC 102 Novelty (BERT): 0.530238748455451
- Combined Prediction Score: 0.5864682951328979
- Mean Citation Score: 312.4161220000001
- Max Citation Score: 344.81528
- Similarity Product: 231.2268983131313

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test