PATENT CLAIM ANALYSIS

Application Number: 15920092
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-09
Patent Classification: ["506", "004000"]

Abstract:
Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

Claim (Index 1):
A method for functional analysis of biological cells, comprising:\n isolating into a monodisperse emulsion microdroplet a single target cell from a plurality of target cell clones of a first cell type and one or more inducer cells from a plurality of inducer cell clones of a second cell type; incubating isolated cells in the monodisperse emulsion microdroplet, wherein the isolated cells comprise the single target cell and the one or more inducer cells; introducing an aqueous solution containing a lysis reagent into said monodisperse emulsion microdroplet, thereby inducing lysis of the isolated cells; capturing RNA released from the isolated cells on a solid surface; and generating a library of hybridized polynucleic acids that comprise a transcript from the isolated cells, wherein the hybridized polynucleic acids are indicative of transcriptional change in the single target cell after the step of incubating the isolated cells.

Metadata:
- Claim Count in Document: 53.0
- Percentile: 90.0
- Lexical Diversity: 1.525
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15159674', '15431660', '15159705', '14734953', '15156217']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5511378939814482
- 35 USC 102 Novelty (BERT): 0.5206584773374741
- Combined Prediction Score: 0.5480899523170508
- Mean Citation Score: 267.570496
- Max Citation Score: 299.69458
- Similarity Product: 214.35917880675672

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test