PATENT CLAIM ANALYSIS

Application Number: 15763585
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-11
Patent Classification: ["435", "440000"]

Abstract:
The present invention provides a construction method of cell models for detecting pyrogens, cell models and pyrogen detection kits. By inducing double-bond breakage in the specific positions of genomes using CRISPR/CAS9, performing directed knock-in of TLR4 and CD14-MD2 on two chromosomes in a cell line based on the principle of homologous recombination and repair, and tracing using green fluorescent protein GFP and red fluorescent protein RFP respectively, we successfully construct the TLR4/CD14/MD2 directed knock-in and fluorescence-traceable cell model; upon the exposure to LPS, the release of IL-6 and TNF-a cytokines in the cell model can be detected by ELISA, Western Blot, mass spectrometry and magnetic bead method; and the cell model has good stability and high sensitivity, and the lowest detection limit can reach 0.005 EU/mL, which is far lower than that of the Limulus amebocyte lysate endotoxin test method (0.025 EU/mL).

Claim (Index 4):
The construction method of  claim 1 , wherein the position where the CD14 gene is knocked-in in step 4) is the first introne of CCR5 gene of chromosome 3.

Metadata:
- Claim Count in Document: 27.0
- Percentile: 90.0
- Lexical Diversity: 1.53922
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['10432236', '15148663', '14343974', '11613866', '13046562']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6226219668485289
- 35 USC 102 Novelty (BERT): 0.5161566649020544
- Combined Prediction Score: 0.6119754366538814
- Mean Citation Score: 247.929142
- Max Citation Score: 255.49191
- Similarity Product: 138.6033609668219

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test