PATENT CLAIM ANALYSIS

Application Number: 16273071
Application Type: Utility
Filing Date: 2019-02
Publication Date: 2019-08
Patent Classification: ["424", "093210"]

Abstract:
Methods for generating cells of the inner ear, e.g., hair cells and supporting cells, from stem cells, e.g., mesenchymal stem cells, are provided, as well as compositions including the inner ear cells. Methods for the therapeutic use of the inner ear cells for the treatment of hearing loss are also described.

Claim (Index 2):
A method of increasing numbers of differentiated inner ear cells in the ear of a subject, the method comprising:\n generating a differentiated inner ear cell, using a method comprising: (i) providing a population of mesenchymal stem cells obtained from mammalian bone marrow; (ii) culturing the mesenchymal stem cell in serum-free medium containing insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), and basic fibroblast growth factor (bFGF); (iii) inducing the stem cell by maintaining said cell in medium comprising neurotrophin-3 (NT-3) or brain derived neurotrophic factor (BDNF) for time sufficient to differentiate into a progenitor cell that expresses Sox2, Pax6, nestin, and musashih; and (iv) culturing the progenitor cell in the presence of a gamma secretase inhibitor, wherein the gamma secretase inhibitor is selected from the group consisting of an arylsulfonamide, a dibenzazepine, a benzodiazepine, N\u2014[N-(3,5-difluorophenacetyl)-L-alanyl]-(S)-phenylglycine t-butyl ester (DAPT), L-685,458, and MK0752, in an amount and for a time sufficient to produce a differentiated inner ear auditory hair cell that expresses myosin VIIa, and espin and optionally expresses one or more of atonal homolog 1 (Atoh1), or jagged 2, thereby producing a differentiated inner ear auditory hair cell, and implanting the differentiated inner ear auditory hair cell into the ear of the subject.

Metadata:
- Claim Count in Document: 7.0
- Percentile: 99.0
- Lexical Diversity: 2.0
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14833919', '13130607', '13454702', '14511911', '12368173']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7020962451631353
- 35 USC 102 Novelty (BERT): 0.6363506283180508
- Combined Prediction Score: 0.6955216834786269
- Mean Citation Score: 375.220134
- Max Citation Score: 622.2921
- Similarity Product: 570.028990182066

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test