PATENT CLAIM ANALYSIS

Application Number: 16297492
Application Type: Utility
Filing Date: 2019-03
Publication Date: 2019-09
Patent Classification: ["435", "091200"]

Abstract:
The invention relates to methods for the detection of a specific sequence of RNA in a cell or tissue sample. The invention also relates to methods to enzymatically manipulate the RNA in a crude cell lysate in a number of applications.

Claim (Index 79):
A method for producing cDNA and amplification thereof for quantification of RNA from at least one biological unit containing RNA, wherein the biological unit is a cell obtained from cell culture, from a tissue sample or from a body fluid, comprising:\n preparing an admixture comprising the biological unit, a deoxyribonuclease and at least one catabolic enzyme, whereby said enzyme is at least proteinase K; incubating the admixture under conditions where the proteinase K and the deoxyribonuclease are active; raising the temperature of the admixture to inactivate the proteinase K and deoxyribonuclease to produce an inactivated admixture; and carrying out one-step RT-PCR on the inactivated admixture.

Metadata:
- Claim Count in Document: 58.0
- Percentile: 99.0
- Lexical Diversity: 1.65385
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15466824', '16001279', '13463624', '12489654', '12122274']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5908329307742819
- 35 USC 102 Novelty (BERT): 0.5640389917630231
- Combined Prediction Score: 0.588153536873156
- Mean Citation Score: 358.697152
- Max Citation Score: 429.02036
- Similarity Product: 419.65910641723633

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test