PATENT CLAIM ANALYSIS

Application Number: 15958354
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "101000"]

Abstract:
A method for producing heparosan is provided. Heparosan is produced by culturing an  Escherichia  bacterium having a heparosan-producing ability and modified so that expression of one or more genes, such as rbsR, rbsK, rbsB, hsrA, glgB, glgX, micF, rcsD, rcsB, ybiX, ybil, ybiJ, ybiC, ybiB, rfaH, nusG, pcoR, pcoS, pcoE, yhcN, yhcO, aaeB, aaeA, aaeX, g1455, alpA, g1453, yrbA, mlaB, mlaC, mlaD, mlaE, mlaF, yrbG, norW, ybjI, ybjJ, ybjK, rybB, yjjY, yjtD, thrL, thrA, thrB, fruA, psuK, ytfT, yjfF, fbp, yagU, paoA, paoB, gsiC, gsiD, yliE, irp2, irp1, bhsA, ycfS, lepB, rnc, era, dapA, gcvR, bcp, hyfA, rpoE, nadB, yfiC, srmB, g1414, g1413, nuoE, nuoF, nuoG, glmZ, hemY, hemX, hemD, rlmL, artQ, artM, artJ, rlmC, ybjO, yejO, yejM, yejL, rpoS, ygbN, ygbM, ygbL, g3798, g3797, g3796, g3795, g3794, g3793, g3792, ryjA, soxR, soxS, yjcC, yjcB, efeU, and efeO, is/are increased in a medium, and collecting heparosan from the medium.

Claim (Index 7):
The bacterium according to  claim 1 , wherein:\n the rbsB gene is a DNA comprising the nucleotide sequence of positions 800 to 1690 of SEQ ID NO: 29, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 800 to 1690 of SEQ ID NO: 29 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rbsK gene is a DNA comprising the nucleotide sequence of positions 1816 to 2745 of SEQ ID NO: 29, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1816 to 2745 of SEQ ID NO: 29 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rbsR gene is a DNA comprising the nucleotide sequence of positions 2749 to 3741 of SEQ ID NO: 29, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2749 to 3741 of SEQ ID NO: 29 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the hsrA gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 3707 to 5134 of SEQ ID NO: 29, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 3707 to 5134 of SEQ ID NO: 29 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the glgB gene is a DNA comprising the nucleotide sequence of positions 989 to 3175 of SEQ ID NO: 34, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 989 to 3175 of SEQ ID NO: 34 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the glgX gene is a DNA comprising the nucleotide sequence of positions 3172 to 5145 of SEQ ID NO: 34, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3172 to 5145 of SEQ ID NO: 34 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rcsB gene is a DNA comprising the nucleotide sequence of positions 3312 to 3962 of SEQ ID NO: 43, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3312 to 3962 of SEQ ID NO: 43 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rcsD gene is a DNA comprising the nucleotide sequence of positions 623 to 3295 of SEQ ID NO: 43, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 623 to 3295 of SEQ ID NO: 43 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the micF gene is a DNA comprising the nucleotide sequence of positions 219 to 311 of SEQ ID NO: 43, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 219 to 311 of SEQ ID NO: 43 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybiX gene is a DNA comprising the nucleotide sequence of positions 718 to 1395 of SEQ ID NO: 37, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 718 to 1395 of SEQ ID NO: 37 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybiI gene is a DNA comprising the nucleotide sequence of positions 1469 to 1735 of SEQ ID NO: 37, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1469 to 1735 of SEQ ID NO: 37 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybiJ gene is a DNA comprising the nucleotide sequence of positions 2000 to 2260 of SEQ ID NO: 37, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2000 to 2260 of SEQ ID NO: 37 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybiC gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2488 to 3574 of SEQ ID NO: 37, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2488 to 3574 of SEQ ID NO: 37 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybiB gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 3715 to 4677 of SEQ ID NO: 37, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 3715 to 4677 of SEQ ID NO: 37 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rfaH gene is a DNA comprising the nucleotide sequence of SEQ ID NO: 46, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of SEQ ID NO: 46 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the nusG gene is a DNA comprising the nucleotide sequence of SEQ ID NO: 48, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of SEQ ID NO: 48 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the pcoR gene is a DNA comprising the nucleotide sequence of positions 128 to 808 of SEQ ID NO: 50, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 128 to 808 of SEQ ID NO: 50 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the pcoS gene is a DNA comprising the nucleotide sequence of positions 805 to 2205 of SEQ ID NO: 50, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 805 to 2205 of SEQ ID NO: 50 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the pcoE gene is a DNA comprising the nucleotide sequence of positions 2423 to 2857 of SEQ ID NO: 50, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2423 to 2857 of SEQ ID NO: 50 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yhcN gene is a DNA comprising the nucleotide sequence of positions 63 to 326 of SEQ ID NO: 54, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 63 to 326 of SEQ ID NO: 54 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yhcO gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 382 to 654 of SEQ ID NO: 54, or a DNA comprising a nucleotide sequence showing an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 382 to 654 of SEQ ID NO: 54 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the aaeB gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 746 to 2713 of SEQ ID NO: 54, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 746 to 2713 of SEQ ID NO: 54 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the aaeA gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2719 to 3651 of SEQ ID NO: 54, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2719 to 3651 of SEQ ID NO: 54 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the aaeX gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 3659 to 3931 of SEQ ID NO: 54, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 3659 to 3931 of SEQ ID NO: 54 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g1455 gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 568 to 1140 of SEQ ID NO: 60, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 568 to 1140 of SEQ ID NO: 60 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the alpA gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 1226 to 1486 of SEQ ID NO: 60, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 1226 to 1486 of SEQ ID NO: 60 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g1453 gene is a DNA comprising the nucleotide sequence of positions 2389 to 2529 of SEQ ID NO: 60, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2389 to 2529 of SEQ ID NO: 60 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yrbA gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 977 to 1246 of SEQ ID NO: 64, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 977 to 1246 of SEQ ID NO: 64 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the mlaB gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 1391 to 1780 of SEQ ID NO: 64, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 1391 to 1780 of SEQ ID NO: 64 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the mlaC gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 1684 to 2319 of SEQ ID NO: 64, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 1684 to 2319 of SEQ ID NO: 64 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the mlaD gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2338 to 2889 of SEQ ID NO: 64, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2338 to 2889 of SEQ ID NO: 64 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the mlaE gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2894 to 3676 of SEQ ID NO: 64, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2894 to 3676 of SEQ ID NO: 64 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the mlaF gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 3684 to 4493 of SEQ ID NO: 64, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 3684 to 4493 of SEQ ID NO: 64 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yrbG gene is a DNA comprising the nucleotide sequence of positions 4703 to 5680 of SEQ ID NO: 64, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 4703 to 5680 of SEQ ID NO: 64 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the norW gene is a DNA comprising the nucleotide sequence of positions 1201 to 2334 of SEQ ID NO: 72, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1201 to 2334 of SEQ ID NO: 72 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybjI gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 117 to 932 of SEQ ID NO: 74, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 117 to 932 of SEQ ID NO: 74 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybjJ gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 932 to 2140 of SEQ ID NO: 74, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 932 to 2140 of SEQ ID NO: 74 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybjK gene is a DNA comprising the nucleotide sequence of positions 2224 to 2760 of SEQ ID NO: 74, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2224 to 2760 of SEQ ID NO: 74 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rybB gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2777 to 2855 of SEQ ID NO: 74, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2777 to 2855 of SEQ ID NO: 74 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yjjY gene is a DNA comprising the nucleotide sequence of positions 124 to 264 of SEQ ID NO: 78, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 124 to 264 of SEQ ID NO: 78 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yjtD gene is a DNA comprising the nucleotide sequence of positions 664 to 1350 of SEQ ID NO: 78, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 664 to 1350 of SEQ ID NO: 78 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the thrL gene is a DNA comprising the nucleotide sequence of positions 1564 to 1629 of SEQ ID NO: 78, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1564 to 1629 of SEQ ID NO: 78 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the thrA gene is a DNA comprising the nucleotide sequence of positions 1711 to 4173 of SEQ ID NO: 78, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1711 to 4173 of SEQ ID NO: 78 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the thrB gene is a DNA comprising the nucleotide sequence of positions 4175 to 5107 of SEQ ID NO: 78, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 4175 to 5107 of SEQ ID NO: 78 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the fruA gene is a DNA comprising the nucleotide sequence of positions 897 to 2588 of SEQ ID NO: 84, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 897 to 2588 of SEQ ID NO: 84 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the psuK gene is a DNA comprising the nucleotide sequence of positions 3165 to 3953 of SEQ ID NO: 84, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3165 to 3953 of SEQ ID NO: 84 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ytfT gene is a DNA comprising the nucleotide sequence of positions 252 to 1277 of SEQ ID NO: 87, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 252 to 1277 of SEQ ID NO: 87 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yjfF gene is a DNA comprising the nucleotide sequence of positions 1264 to 2259 of SEQ ID NO: 87, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1264 to 2259 of SEQ ID NO: 87 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the fbp gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2292 to 3290 of SEQ ID NO: 87, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2292 to 3290 of SEQ ID NO: 87 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yagU gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 117 to 731 of SEQ ID NO: 91, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 117 to 731 of SEQ ID NO: 91 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the paoA gene is a DNA comprising the nucleotide sequence of positions 1149 to 1838 of SEQ ID NO: 91, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1149 to 1838 of SEQ ID NO: 91 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the paoB gene is a DNA comprising the nucleotide sequence of positions 1835 to 2791 of SEQ ID NO: 91, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1835 to 2791 of SEQ ID NO: 91 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the gsiC gene is a DNA comprising the nucleotide sequence of positions 264 to 1184 of SEQ ID NO: 95, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 264 to 1184 of SEQ ID NO: 95 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the gsiD gene is a DNA comprising the nucleotide sequence of positions 1187 to 2098 of SEQ ID NO: 95, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1187 to 2098 of SEQ ID NO: 95 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yliE gene is a DNA comprising the nucleotide sequence of positions 2276 to 4624 of SEQ ID NO: 95, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2276 to 4624 of SEQ ID NO: 95 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the irp2 gene is a DNA comprising the nucleotide sequence of SEQ ID NO: 100, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of SEQ ID NO: 100 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the irp1 gene is a DNA comprising the nucleotide sequence of SEQ ID NO: 102, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of SEQ ID NO: 102 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the bhsA gene is a DNA comprising the nucleotide sequence of positions 440 to 697 of SEQ ID NO: 104, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 440 to 697 of SEQ ID NO: 104 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ycfS gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 779 to 1741 of SEQ ID NO: 104, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 779 to 1741 of SEQ ID NO: 104 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the lepB gene is a DNA comprising the nucleotide sequence of positions 1344 to 2318 of SEQ ID NO: 107, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1344 to 2318 of SEQ ID NO: 107 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rnc gene is a DNA comprising the nucleotide sequence of positions 2590 to 3270 of SEQ ID NO: 107, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2590 to 3270 of SEQ ID NO: 107 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the era gene is a DNA comprising the nucleotide sequence of positions 3267 to 4172 of SEQ ID NO: 107, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3267 to 4172 of SEQ ID NO: 107 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the dapA gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 858 to 1736 of SEQ ID NO: 111, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 858 to 1736 of SEQ ID NO: 111 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the gcvR gene is a DNA comprising the nucleotide sequence of positions 1882 to 2454 of SEQ ID NO: 111, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1882 to 2454 of SEQ ID NO: 111 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the bcp gene is a DNA comprising the nucleotide sequence of positions 2454 to 2924 of SEQ ID NO: 111, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2454 to 2924 of SEQ ID NO: 111 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the hyfA gene is a DNA comprising the nucleotide sequence of positions 3177 to 3794 of SEQ ID NO: 111, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3177 to 3794 of SEQ ID NO: 111 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rpoE gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 355 to 930 of SEQ ID NO: 116, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 355 to 930 of SEQ ID NO: 116 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the nadB gene is a DNA comprising the nucleotide sequence of positions 1338 to 2960 of SEQ ID NO: 116, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1338 to 2960 of SEQ ID NO: 116 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yfiC gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2945 to 3682 of SEQ ID NO: 116, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2945 to 3682 of SEQ ID NO: 116 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the srmB gene is a DNA comprising the nucleotide sequence of positions 3814 to 5148 of SEQ ID NO: 116, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3814 to 5148 of SEQ ID NO: 116 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g1414 gene is a DNA comprising the nucleotide sequence of positions 28 to 699 of SEQ ID NO: 121, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 28 to 699 of SEQ ID NO: 121 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g1413 gene is a DNA comprising the nucleotide sequence of positions 831 to 1157 of SEQ ID NO: 121, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 831 to 1157 of SEQ ID NO: 121 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the nuoE gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 796 to 1296 of SEQ ID NO: 124, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 796 to 1296 of SEQ ID NO: 124 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the nuoF gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 1293 to 2630 of SEQ ID NO: 124, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 1293 to 2630 of SEQ ID NO: 124 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the nuoG gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2683 to 5409 of SEQ ID NO: 124, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2683 to 5409 of SEQ ID NO: 124 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the glmZ gene is a DNA comprising the nucleotide sequence of positions 357 to 563 of SEQ ID NO: 128, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 357 to 563 of SEQ ID NO: 128 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the hemY gene is a DNA comprising the nucleotide sequence of positions 611 to 1807 of SEQ ID NO: 128, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 611 to 1807 of SEQ ID NO: 128 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the hemX gene is a DNA comprising the nucleotide sequence of positions 1810 to 2991 of SEQ ID NO: 128, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1810 to 2991 of SEQ ID NO: 128 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the hemD gene is a DNA comprising the nucleotide sequence of positions 3013 to 3753 of SEQ ID NO: 128, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3013 to 3753 of SEQ ID NO: 128 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rlmL gene is a DNA comprising the nucleotide sequence of positions 571 to 2679 of SEQ ID NO: 132, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 571 to 2679 of SEQ ID NO: 132 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the artQ gene is a DNA comprising the nucleotide sequence of positions 386 to 1102 of SEQ ID NO: 134, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 386 to 1102 of SEQ ID NO: 134 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the artM gene is a DNA comprising the nucleotide sequence of positions 1102 to 1770 of SEQ ID NO: 134, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1102 to 1770 of SEQ ID NO: 134 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the artJ gene is a DNA comprising the nucleotide sequence of positions 2061 to 2792 of SEQ ID NO: 134, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2061 to 2792 of SEQ ID NO: 134 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rlmC gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2991 to 4118 of SEQ ID NO: 134, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2991 to 4118 of SEQ ID NO: 134 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ybjO gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 4159 to 4647 of SEQ ID NO: 134, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 4159 to 4647 of SEQ ID NO: 134 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yejO gene is a DNA comprising the nucleotide sequence of positions 216 to 2807 of SEQ ID NO: 140, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 216 to 2807 of SEQ ID NO: 140 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yejM gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 3061 to 4821 of SEQ ID NO: 140, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 3061 to 4821 of SEQ ID NO: 140 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yejL gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 4841 to 5068 of SEQ ID NO: 140, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 4841 to 5068 of SEQ ID NO: 140 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the rpoS gene is a DNA comprising the nucleotide sequence of positions 318 to 1310 of SEQ ID NO: 144, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 318 to 1310 of SEQ ID NO: 144 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ygbN gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 1404 to 2768 of SEQ ID NO: 144, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 1404 to 2768 of SEQ ID NO: 144 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ygbM gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 2857 to 3633 of SEQ ID NO: 144, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 2857 to 3633 of SEQ ID NO: 144 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ygbL gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 3638 to 4276 of SEQ ID NO: 144, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 3638 to 4276 of SEQ ID NO: 144 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g3798 gene is a DNA comprising the nucleotide sequence of positions 615 to 1268 of SEQ ID NO: 149, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 615 to 1268 of SEQ ID NO: 149 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g3797 gene is a DNA comprising the nucleotide sequence of positions 1368 to 2219 of SEQ ID NO: 149, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1368 to 2219 of SEQ ID NO: 149 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g3796 gene is a DNA comprising the nucleotide sequence of positions 2257 to 2748 of SEQ ID NO: 149, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 2257 to 2748 of SEQ ID NO: 149 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g3795 gene is a DNA comprising the nucleotide sequence of positions 3021 to 3203 of SEQ ID NO: 149, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3021 to 3203 of SEQ ID NO: 149 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g3794 gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 3470 to 4051 of SEQ ID NO: 149, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 3470 to 4051 of SEQ ID NO: 149 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g3793 gene is a DNA comprising the nucleotide sequence of positions 4280 to 4480 of SEQ ID NO: 149, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 4280 to 4480 of SEQ ID NO: 149 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the g3792 gene is a DNA comprising the nucleotide sequence of positions 4520 to 4717 of SEQ ID NO: 149, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 4520 to 4717 of SEQ ID NO: 149 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the ryjA gene is a DNA comprising the nucleotide sequence of positions 657 to 796 of SEQ ID NO: 157, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 657 to 796 of SEQ ID NO: 157 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the soxR gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 790 to 1254 of SEQ ID NO: 157, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 790 to 1254 of SEQ ID NO: 157 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the soxS gene is a DNA comprising the nucleotide sequence of positions 1340 to 1663 of SEQ ID NO: 157, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1340 to 1663 of SEQ ID NO: 157 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yjcC gene is a DNA comprising the complementary sequence of the nucleotide sequence of positions 1666 to 3252 of SEQ ID NO: 157, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the complementary sequence of the nucleotide sequence of positions 1666 to 3252 of SEQ ID NO: 157 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the yjcB gene is a DNA comprising the nucleotide sequence of positions 3682 to 3963 of SEQ ID NO: 157, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 3682 to 3963 of SEQ ID NO: 157 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; the efeU gene is a DNA comprising the nucleotide sequence of positions 753 to 1583 of SEQ ID NO: 162, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 753 to 1583 of SEQ ID NO: 162 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium; and the efeO gene is a DNA comprising the nucleotide sequence of positions 1641 to 2768 of SEQ ID NO: 162, or a DNA comprising a nucleotide sequence having an identity of 90% or higher to the nucleotide sequence of positions 1641 to 2768 of SEQ ID NO: 162 and is able to increase heparosan-producing ability of an  Escherichia  bacterium having heparosan-producing ability when expression amount thereof is increased in the bacterium.

Metadata:
- Claim Count in Document: 12.0
- Percentile: 91.0
- Lexical Diversity: 1.78621
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15082464', '14467459', '14990047', '15630115', '15717042']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6400769516019732
- 35 USC 102 Novelty (BERT): 0.6249587776188958
- Combined Prediction Score: 0.6385651342036655
- Mean Citation Score: 407.596036
- Max Citation Score: 648.49677
- Similarity Product: 523.1603898161762

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test