PATENT CLAIM ANALYSIS

Application Number: 16235524
Application Type: Utility
Filing Date: 2018-12
Publication Date: 2019-07
Patent Classification: ["514", "235200"]

Abstract:
The invention relates to methods of reducing the level of 2-hydroxyglutarate in cells by administration of an inhibitor of mutant isocitrate dehydrogenase-1.

Claim (Index 1):
A method of reducing the production of 2-hydroxyglutarate (2-HG) in a cell producing 2-HG (\u201c2-HG producing cell\u201d),\n wherein the method comprises the step of contacting the 2-HG producing cell with an inhibitor of mutant isocitrate dehydrogenase-1 protein (mt-IDH1), wherein the inhibitor of mt-IDH1 has an IC 50  value of less than 1 micromolar against HCT116 cells harboring mt-IDH1 (\u201cHCT116 mt-IDH1 cells\u201d) selected from the group consisting of HCT116 isogenic IDH1-R132H cells and HCT116 isogenic IDH1-R132C cells; wherein the IC 50  value of the inhibitor of mt-IDH1 is determined by a cellular 2-HG assay using HCT116 mt-IDH1 cells according to the following steps:\n a. culturing the HCT116 mt-IDH1 cells in a growth media comprising McCoy's 5A, 10% fetal bovine serum, 1\u00d7 antibiotic-antimycotic solution, and 0.3 mg/mL G418 in 5% CO 2  in an incubator at 37\u00b0 C.; \n b. trypsinizing and resuspending the HCT116 mt-IDH1 cells in an assay media comprising McCoy's 5A with no L-glutamine, 10% fetal bovine serum, 1\u00d7 antibiotic-antimycotic solution, and 0.3 mg/mL G418; \n c. obtaining an aliquot of 10,000 cells/100 \u03bcL and transferring the aliquot to each well of a clear 96-well tissue culture plate; \n d. incubating the cells in the culture plate in 5% CO 2  at 37\u00b0 C. in an incubator overnight; \n e. obtaining an aliquot of 50 \u03bcL of an assay media comprising the inhibitor of mt-IDH1, and adding the aliquot to each well of the clear 96-well tissue culture plate and maintaining the culture plate in 5% CO 2  at 37\u00b0 C. in an incubator for 24 hours; \n f. removing the media from each well of the clear 96-well tissue culture plate and adding 150 \u03bcL of a methanol/water mixture (80/20 v/v) to each well; \n g. maintaining the plate at \u221280\u00b0 C. freezer for 12 hours; \n h. obtaining an aliquot of 125 \u03bcL of extracted methanol/water mixture and analyzing the aliquot by a high-throughput-mass spectrometry to determine the cellular 2-HG level; and \n i. determining the IC 50  value of the inhibitor of mt-IDH1 for the HCT116 mt-IDH1 cells.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 98.0
- Lexical Diversity: 1.15
- Patent Class: 514.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15964844', '15712446', '14858165', '15568051', '14858159']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7634277186912387
- 35 USC 102 Novelty (BERT): 0.5246621768459705
- Combined Prediction Score: 0.7395511645067119
- Mean Citation Score: 313.153344
- Max Citation Score: 326.39804
- Similarity Product: 210.75472083538293

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test