PATENT CLAIM ANALYSIS

Application Number: 15744054
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["514", "019300"]

Abstract:
The present invention refers to new binding proteins based on di-ubiquitin modified in 12, 13, or 14 positions selected from positions R42, I44, H68, V70, R72, L73, R74, K82, L84, Q138, K139, E140, S141, and T142 of di-ubiquitin and being able to bind specifically with high affinity to selected targets. Furthermore, the invention provides a method for the generation of said binding proteins. In addition, libraries encoding for said binding proteins based on di-ubiquitin muteins modified in 12, 13, or 14 positions selected from R42, I44, H68, V70, R72, L73, R74, K82, L84, Q138, K139, E140, S141, and T142 are provided.

Claim (Index 6):
The method of  claim 5 , comprising:\n (a) expressing a nucleic acid library on the surface of a host cell, wherein the nucleic acid library encodes a plurality of di-ubiquitin muteins, and further wherein the plurality of di-ubiquitin muteins comprise substitutions of 12-14 amino acid residues selected from the group consisting of amino acid positions R42, I44, H68, V70, R72, L73, R74, K82, L84, Q138, K139, E140, S141, and T142 of SEQ ID NO: 4; (b) contacting the members of the expressed nucleic acid library with a target; and (c) identifying one or more members of the expressed library that bind to the target; (d) screening the one or more members of the expressed library that bind to the target to identify a binding protein that binds to said target with a specific binding affinity of at least 1000 nM.

Metadata:
- Claim Count in Document: 54.0
- Percentile: 86.0
- Lexical Diversity: 2.22951
- Patent Class: 514.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14126358', '13144809', '14442376', '12293715', '13514133']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.719690676378144
- 35 USC 102 Novelty (BERT): 0.5238257160633567
- Combined Prediction Score: 0.7001041803466652
- Mean Citation Score: 294.255452
- Max Citation Score: 307.40186
- Similarity Product: 217.79422631167648

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test