PATENT CLAIM ANALYSIS

Application Number: 15746398
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["435", "325000"]

Abstract:
Factors for extending the ability of isolated pluripotent stem cells to generate extraembryonic lineages in vivo, following in vitro culture, herein, chemical extenders of pluripotency (CEP). Methods of extending the ability of a pluripotent cell to generate embryonic and extraembryonic lineages. The cell to be reprogrammed is contacted with effective amounts of the CEPs for a sufficient period of time to reprogram the cell into a chemically induced extended pluripotent cell (ciEPSC). ciEPSC are identified as an extended pluripotent cell based on properties including: (i) morphologically and (ii) functionally for example, based on their ability contribute to both TE and ICM, in vivo. The ciEPSCs can be cultured or induced to differentiate into cells of a desired type, and used in a number of applications, including but not limited to cell therapy and tissue engineering.

Claim (Index 13):
A method of inducing extended pluripotency in donor cells selected from a pluripotent, partially or completely differentiated cell, the method comprising:\n culturing the donor cells with the composition of  claim 1  for a period of time effective to induce extended pluripotency; and optionally wherein the donor cells are selected from the group consisting of embryonic stem cells, induced pluripotent stem cells, multipotent stem cells, cells of hematological origin, cells of embryonic origin, skin derived cells, fibroblasts, adipose cells, epithelial cells, endothelial cells, mesenchymal cells, parenchymal cells, neurological cells, and connective tissue cells, and more preferably the donor cells are selected from the group consisting of mouse embryonic stem cells, human embryonic stem cells, and induced pluripotent stem cells; the donor cells are cultured in a reprogramming medium comprising the CEPs for a period ranging from 9-20 days; the donor cells are seeded as single cells or as small colonies; and/or the donor cells are seeded as single cells, the method further comprising culturing the cells in cell culture medium comprising the ROCK inhibitor for a period of time ranging from 12 to 48 hours, preferably 24-48 hours, before culture in medium comprising LCDM.

Metadata:
- Claim Count in Document: 32.0
- Percentile: 86.0
- Lexical Diversity: 1.81395
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14904195', '09436164', '12108852', '14735841', '12164795']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5608859384607
- 35 USC 102 Novelty (BERT): 0.5190373870399886
- Combined Prediction Score: 0.5567010833186289
- Mean Citation Score: 321.480656
- Max Citation Score: 338.71844
- Similarity Product: 273.77525946866507

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test