PATENT CLAIM ANALYSIS

Application Number: 16153052
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-01
Patent Classification: ["435", "440000"]

Abstract:
This invention relates to the use of CRISPR nucleic acids to screen for essential and non-essential genes and expendable genomic islands in bacteria, archaea, algae and/or yeast, to kill bacteria, archaea, algae and/or yeast, to identify the phenotype of a gene or genes, and/or to screen for reduced genome size and/or a gene deletion in bacteria, archaea, algae and/or yeast.

Claim (Index 1):
A method of modifying a mixed population of bacterial cells having a first bacterial population and a second bacterial population, comprising:\n introducing into the mixed population of bacterial cells a heterologous nucleic acid construct comprising a CRISPR array (crRNA, crDNA) comprising (5\u2032 to 3\u2032) a repeat-spacer-repeat sequence or at least one repeat-spacer sequence, wherein the spacer of the repeat-spacer-repeat sequence or the at least one repeat-spacer sequence comprises a nucleotide sequence that is substantially complementary to a target sequence in a genome of the first bacterial population, wherein the CRISPR array is operable with a Cas nuclease to kill one or more bacterial cells within the first bacterial population, wherein the Cas nuclease is endogenous to the one or more bacterial cells; provided the following are not introduced into the mixed population of bacterial cells: an exogenous Cas nuclease polypeptide that recognizes the CRIPSR array, and an exogenous nucleic acid construct encoding the exogenous Cas nuclease polypeptide.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 97.0
- Lexical Diversity: 1.89189
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15167727', '15032985', '15160405', '15460962', '15507176']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6194946591666107
- 35 USC 102 Novelty (BERT): 0.6766058371952843
- Combined Prediction Score: 0.6252057769694781
- Mean Citation Score: 471.122638
- Max Citation Score: 734.0977
- Similarity Product: 643.7099831880868

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test