PATENT CLAIM ANALYSIS

Application Number: 16377344
Application Type: Utility
Filing Date: 2019-04
Publication Date: 2019-08
Patent Classification: ["435", "006100"]

Abstract:
A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.

Claim (Index 36):
A method comprising:\n a) preparing a blood collection tube including at least, or about, 200 grams per liter of a composition formulated for stabilizing cell-free nucleic acids within a blood sample, the composition including:\n i) from about 0.5% to about 20% by weight one or more nuclease inhibitors; \n ii) from about 50 to about 500 grams per liter of one or more preservative agents; and \n iii) one or more solvents; \n b) drawing blood into the evacuated blood collection tube including the composition; c) sending the blood collection tube and composition located therein to a remote location for collection of a blood sample that contains cell-free nucleic acids that are stabilized by the composition d) isolating the cell free nucleic acids from the stabilized blood sample; and e) testing the isolated cell free nucleic acids from the stabilized blood sample to identify the presence, absence or severity of a disease state by performing one or more of the following: polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), quantitative real time polymerase chain reaction (Q-PCR), gel electrophoresis, capillary electrophoresis, mass spectrometry, fluorescence detection, ultraviolet spectrometry, DNA hybridization, allele specific polymerase chain reaction, polymerase cycling assembly (PCA), asymmetric polymerase chain reaction, linear after the exponential polymerase chain reaction (LATE-PCR), helicase-dependent amplification (HDA), hot-start polymerase chain reaction, intersequence-specific polymerase chain reaction (ISSR), inverse polymerase chain reaction, ligation mediated polymerase chain reaction, methylation specific polymerase chain reaction (MSP), multiplex polymerase chain reaction, nested polymerase chain reaction, solid phase polymerase chain reaction, or any combination thereof; and wherein the collected blood sample is storable for at least, or about 7 days without cell lysis and without cell-free nucleic acid degradation of the blood sample due to DNase and RNase activity after blood collection.

Metadata:
- Claim Count in Document: 59.0
- Percentile: 100.0
- Lexical Diversity: 1.6
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15894077', '15937446', '15601682', '14071969', '12704030']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6472067017097293
- 35 USC 102 Novelty (BERT): 0.5799979079048099
- Combined Prediction Score: 0.6404858223292373
- Mean Citation Score: 355.55956000000015
- Max Citation Score: 413.49518
- Similarity Product: 347.93622401440496

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test