PATENT CLAIM ANALYSIS

Application Number: 15961886
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "440000"]

Abstract:
A method for knocking out a human RSPO2 gene targeted with CRISPR-Cas9 specificity includes steps of: 1) designing the sgRNA of the human RSPO2 gene targeted; and 2) constructing a CRISPR-Cas9 recombinant lentivirus vector for knocking out the RSPO2 gene. A method for preparing a lentiviral-packaged system for knocking out a human RSPO2 gene targeted with CRISPR-Cas9 specificity includes steps of: 1) designing the sgRNA of the human RSPO2 gene targeted; 2) constructing a CRISPR-Cas9 recombinant lentivirus vector for knocking out the RSPO2 gene; and 3) processing the CRISPR-Cas9 recombinant lentivirus vector for knocking out the sgRNA of the human RSPO2 gene with lentiviral packaging, so as to obtain the lentiviral-packaged system.

Claim (Index 3):
The method, as recited in  claim 2 , wherein the first preset conditions comprise:\n (1) a length of the sgRNA is 20 nucleotide sequences; (2) a sgRNA target on the RSPO2 gene locates in an exon thereof; (3) the sgRNA target on the RSPO2 gene locates in a functional domain thereof; (4) 5\u2032-NGG is selected for PAM of a target sequence; (5) a sgRNA target sequence is started at G to ensure an effective U6 promoter of a vector; and (6) a format of the sgRNA target sequence is as follows: 5\u2032-G-(19N)-NGG-3\u2032 only when the sgRNA target sequence starts at G; or 5\u2032-(20N)-NGG-3\u2032; wherein, 19N or 20N refers to 19 or 20 nucleotide sequences of the sgRNA target.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 91.0
- Lexical Diversity: 2.97619
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['15179912', '14183486', '15665201', '14258458', '14259420']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5110721210402766
- 35 USC 102 Novelty (BERT): 0.5314059916372093
- Combined Prediction Score: 0.5131055080999698
- Mean Citation Score: 306.6951160000001
- Max Citation Score: 313.19348
- Similarity Product: 176.76246661531923

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test