PATENT CLAIM ANALYSIS

Application Number: 16141534
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-03
Patent Classification: ["435", "007900"]

Abstract:
Compounds useful as labels with properties comparable to known fluorescent compounds. The compounds are conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.

Claim (Index 12):
A method of labeling at least one biomolecule, the method comprising providing a composition comprising at least one excipient and a compound in an effective concentration to label at least one biomolecule under conditions sufficient for labeling the biomolecule with the compound, where the compound is selected from the group consisting of where each of R 1 , R 2 , R 5 , and R 6  is the same or different and is independently selected from the group consisting of an aliphatic, heteroaliphatic, sulfoalkyl, heteroaliphatic with terminal SO 3 , a PEG group P\u2014Z where P is selected from an ethylene glycol group, a diethylene glycol group, and a polyethylene glycol group, where the polyethylene glycol group is (CH 2 CH 2 O) s , where s is an integer from 3-6 inclusive, a sulfonamide group -L-SO 2 NH\u2014P\u2014Z, and a caboxamide group -L-CONH\u2014P\u2014Z, where Z is selected from H, CH 3 , a CH 3  group, an alkyl group, or a heteroalkyl group;\n each of R 7 , R 8 , R 11 , R 12 , R 13 , and R 14  is the same or different and is independently selected from the group consisting of H, SO 3 , a PEG group P\u2014Z where P is selected from an ethylene glycol group, a diethylene glycol group, and a polyethylene glycol group, where the polyethylene glycol group is (CH 2 CH 2 O) s , where s is an integer from 3-6 inclusive, a sulfonamide-containing group -L-SO 2 NH\u2014P\u2014Z, and a carboxamide-containing group -L-CONH\u2014P\u2014Z, where Z is selected from H, CH 3 , a CH 3  group, an alkyl group, or a heteroalkyl group; \n X is selected from \u2014OH, \u2014SH, \u2014NH 2 , \u2014NH\u2014NH 2 , \u2014F, \u2014Cl, \u2014Br, \u2014I, \u2014NHS (hydroxysuccinimidyl/sulfosuccinimidyl), \u2014O-TFP (2,3,5,6-tetrafluorophenoxy), \u2014O-STP (4-sulfo-2,3,5,6-tetrafluorophenoxy), \u2014O-benzotriazole, -benzotriazole, imidazole, azide, \u2014O-carbodiimide, \u2014NR-L-OH, \u2014NR-L-O-phosphoramidite, \n \u2014NR-L-SH, \u2014NR-L-NH 2 , \u2014NR-L-NH\u2014NH 2 , \u2014NR-L-CO 2 H, \u2014NR-L-CO\u2014NHS, \u2014NR-L-CO-STP, \u2014NR-L-CO-TFP, \u2014NR-L-CO-benzotriazole, \u2014NR-L-CHO, \u2014NR-L-maleimide, \u2014NR-L-NH\u2014CO\u2014CH 2 \u2014I, or \u2014NR-L-NH\u2014CO\u2014CH 2 \u2014Br wherein R is \u2014H or an aliphatic or heteroaliphatic group; L is selected from a divalent linear (\u2014(CH 2 ) t \u2014, t=0 to 15), crossed, or cyclic alkyl group optionally substituted by at least one oxygen atom and/or sulfur atom;\n Kat is a number of Na + , K + , Ca 2+ , ammonia, or other cation(s) needed to compensate the negative charge brought by the cyanine; m is an integer from 0 to 5 inclusive; p is an integer from 1 to 6 inclusive; \n each of R3 and R4 is the same or different and is independently hydrogen, an aliphatic group, a heteroaliphatic group, or a PEG group P\u2014Z where P is selected from an ethylene glycol group, a diethylene glycol group, and a polyethylene glycol group, where the polyethylene glycol group is (CH 2 CH 2 O) s , where s is an integer from 3-6 inclusive, and Z is selected from H, a CH 3  group, an alkyl group, or a heteroalkyl group; or R3 and R4 together form a cyclic structure where R3 and R4 are joined using a divalent structural element selected from the group consisting of \u2014(CH 2 ) q \u2014, \u2014(CH 2 ) q O(CH 2 ) q\u2032 \u2014, \n (CH 2 ) q S(CH 2 ) q\u2032 \u2014, \u2014(CH 2 ) q CH\u2550CH\u2014, \u2014OCH\u2550CH\u2014 where each of q and q\u2032 is the same or different and is a integer from 2 to 6 inclusive; and Y is selected from the group consisting of hydrogen, alkyl, sulfoalkyl, fluorine, chlorine, bromine, a substituted or unsubstituted aryl-, phenoxy-, phenylmercapto function, and a PEG group P\u2014Z where P is selected from an ethylene glycol group, a diethylene glycol group, and a polyethylene glycol group, where the polyethylene glycol group is (CH 2 CH 2 O) s , where s is an integer from 3-6 inclusive, and Z is selected from H, a CH 3  group, an alkyl group, or a heteroalkyl group.

Metadata:
- Claim Count in Document: 17.0
- Percentile: 97.0
- Lexical Diversity: 1.44118
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13571858', '14966437', '13778678', '15641376', '15493208']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7151938523566834
- 35 USC 102 Novelty (BERT): 0.6612124547688817
- Combined Prediction Score: 0.7097957125979032
- Mean Citation Score: 655.771748
- Max Citation Score: 872.1003400000003
- Similarity Product: 607.724267096311

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test