PATENT CLAIM ANALYSIS

Application Number: 15907470
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-09
Patent Classification: ["435", "440000"]

Abstract:
The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GOI genome target location and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts from that point on.

Claim (Index 1):
A method for creating a mammalian cell bank for cell line development comprising the following steps:\n a) providing a recombinant mammalian cell comprising (i) a genomic region that is transcriptionally active during suspension culture of said recombinant cell in a serum free culture medium (ii) a recombinant template DNA construct integrated at said genomic region, said recombinant template DNA construct having a region containing elements needed for expression of a template protein of interest and one or several sequence elements enabling the introduction of a donor DNA construct into said template DNA construct; b) based on said recombinant cell or a cell population derived thereof, generating one or several candidate cells or cell populations; c) measuring production traits of said generated candidate cells or cell populations and selecting a top candidate cell or cell population having improved characteristics for production of said template protein of interest; and d) from said top candidate cell or cell population creating a cell bank for cell line development, wherein said donor DNA construct comprises a region encoding a desired protein of interest belonging to the same class as the template protein of interest, and wherein expression of the template protein of interest is exchanged for expression of the desired protein of interest by using an expression vector to introduce said donor DNA construct into said genomic region of a cell or cell population derived from said cell bank.

Metadata:
- Claim Count in Document: 24.0
- Percentile: 88.0
- Lexical Diversity: 1.38462
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14967689', '14092786', '13752647', '12793898', '12507252']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5850877419015902
- 35 USC 102 Novelty (BERT): 0.5203455968878083
- Combined Prediction Score: 0.578613527400212
- Mean Citation Score: 308.898376
- Max Citation Score: 313.97794
- Similarity Product: 228.5527857883835

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test