PATENT CLAIM ANALYSIS

Application Number: 15949457
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-10
Patent Classification: ["424", "422000"]

Abstract:
Described herein are preparations and applications of gellan gum hydrogel for cell culture and biomedical applications, including 2D coating culture, 3D cell culture, and injection. The gellan gum materials for such application include water soluble low acyl gellan gum, high acyl gellan gum, modified gellan gum and a mixture of gellan gum mixture with other chemical/biological molecules.

Claim (Index 21):
A method for forming a polysaccharide hydrogel according to  claim 1 , the method comprising the steps of:\n dissolving water-soluble gellan gum polymers in a water based solvent with a solid content higher than 0.001% w/v at a temperature ranging from about 4\u00b0 C. to about 99\u00b0 C.; heating the solution to a temperature of about 100\u00b0 C. or higher and at a pressure of about 1 psi or higher for 3 minutes or longer; and reticulation at a temperature ranging from about 4\u00b0 C. to about 60\u00b0 C. by directly mixing the solution with phosphate buffer (PBS), cell culture media or ionic solutions to trigger the polysaccharide hydrogel formation,\n wherein a storage modulus (G\u2032) of the polysaccharide hydrogel increases upon mixing and surpasses about 10 Pa within 30 minutes such that the system sustains bioactive molecules suspended within its hydrogel matrix for 3D growth; and \n adding chemicals or bioactive molecules such that the chemicals or bioactive molecules are in contact with, adhered to, suspended, embedded or entrapped in the polysaccharide hydrogel formed.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 91.0
- Lexical Diversity: 1.68421
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14782812', '13637588', '15778380', '15746193', '11611570']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6823019126398907
- 35 USC 102 Novelty (BERT): 0.5342780605549743
- Combined Prediction Score: 0.6674995274313991
- Mean Citation Score: 250.40783
- Max Citation Score: 340.82526
- Similarity Product: 218.55627495693685

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test