PATENT CLAIM ANALYSIS

Application Number: 15948798
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["506", "004000"]

Abstract:
Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

Claim (Index 7):
A method of genome engineering, the method comprising:\n b) contacting a population of cells with a polynucleotide, wherein each cell comprises a first target nucleic acid, a second target nucleic acid, and a nucleic acid-guided nuclease, wherein the polynucleotide comprises\n 1) an editing cassette comprising:\n i) a modified first target nucleic acid sequence; \n ii) a first protospacer adjacent motif (PAM) mutation; \n iii) a first guide nucleic acid sequence targeting the first target nucleic acid and compatible with the nucleic acid-guided nuclease; and \n \n 2) a recorder cassette comprising\n i) a barcode for tracking and identifying the modified first target nucleic acid sequence; and \n ii) a second guide nucleic acid sequence targeting the second target nucleic acid and compatible with the nucleic acid-guided nuclease; \n \n b) allowing the first guide nucleic acid sequence, the second guide nucleic acid sequence, and the nucleic acid-guided nuclease to create a genome edit within the first target nucleic acid and the second target nucleic acid; and c) using the PAM mutation to enrich for cells comprising the genome edit within the first target nucleic acid and second target nucleic acid; wherein the nucleic acid-guided nuclease comprises amino acid sequence SEQ ID NO: 7.

Metadata:
- Claim Count in Document: 50.0
- Percentile: 91.0
- Lexical Diversity: 2.07407
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15632222', '15948793', '15632001', '15631989', '15896433']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.614346921424781
- 35 USC 102 Novelty (BERT): 0.6915354022703152
- Combined Prediction Score: 0.6220657695093343
- Mean Citation Score: 534.756
- Max Citation Score: 702.1871
- Similarity Product: 695.3629521627902

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test