PATENT CLAIM ANALYSIS

Application Number: 16056310
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-12
Patent Classification: ["435", "440000"]

Abstract:
Described herein are methods and vectors for rational, multiplexed manipulation of chromosomes within open reading frames (e.g., in protein libraries) or any segment of a chromosome in a cell or population of cells, in which various CRISPR systems are used.

Claim (Index 20):
A method for tracking a multitude of genome edits in a cell population, comprising:\n a) introducing into a first population of cells at least one first vector that encodes: (i) at least one editing cassette wherein the editing cassette comprises a) a region homologous to a target region of a nucleic acid and comprising a mutation of at least one nucleotide relative to the target region, and b) a mutation preventing nuclease binding at a PAM or spacer; (ii) at least one promoter; and (iii) a region encoding at least one guide RNA comprising a region complementary to a portion of the target region, thereby producing a second population of cells that comprise the at least one first vector; b) maintaining the second population of cells under conditions in which a nuclease is expressed thereby producing a cell library that does not comprise the mutation preventing nuclease binding at the PAM or spacer; c) selecting for viable cells produced in (b) resulting in an edited cell library; and d) tracking the multitude of genome edits in the edited cell library by PCR or sequencing.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 96.0
- Lexical Diversity: 1.24324
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15948785', '15116616', '15630909', '15948789', '15948798']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.776330769904248
- 35 USC 102 Novelty (BERT): 0.6711929096657235
- Combined Prediction Score: 0.7658169838803955
- Mean Citation Score: 682.8296280000002
- Max Citation Score: 752.7857700000003
- Similarity Product: 640.0487915168436

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test