PATENT CLAIM ANALYSIS

Application Number: 16149918
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-02
Patent Classification: ["435", "325000"]

Abstract:
The present invention provides for methods of isolating a stem cell or cell derived therefrom from a mixture of cells, for example, a mixture of adherent cells in culture. Cell isolation is achieved by the application of selective detachment forces.

Claim (Index 10):
A method of isolating a cell of interest from a mixture of animal cells adhered to a substrate, the method comprising:\n a) growing the mixture of animal cells on a substrate in culture such that the cells in the mixture of cells are adhered to the substrate; and b) subjecting the mixture of adhered cells comprising the cell of interest and at least one other cell type, which is different from the cell of interest, to a detachment force that provides a wall shear stress in a range wherein said detachment force selectively detaches the at least one other cell type in the mixture of adhered cells and the cell of interest is not detached from the substrate, thereby isolating the cell of interest from the mixture of cells adhered to the substrate, wherein: a) the cell of interest is a hiPSC-derived cardiomyocyte and the at least one other cell type is an hiPSC and the detachment force provides a wall shear stress in a range of 70-160 dynes/cm 2 ; b) the cell of interest is a fibroblast and the at least one other cell type is a hiPSC and/or a hESC and the detachment force provides a wall shear stress in a range of 70-160 dynes/cm 2 ; and/or c) the cell of interest is a partially differentiated and/or spontaneously differentiated progeny of a hiPSC and the at least one other cell type is an undifferentiated hiPSC and the detachment force provides a wall shear stress in a range of 70-160 dynes/cm 2 .

Metadata:
- Claim Count in Document: 1.0
- Percentile: 97.0
- Lexical Diversity: 1.33333
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['16148784', '14128547', '13997014', '13068643', '14637089']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6071720828405819
- 35 USC 102 Novelty (BERT): 0.7251200750017605
- Combined Prediction Score: 0.6189668820566998
- Mean Citation Score: 488.05858200000006
- Max Citation Score: 803.4739999999997
- Similarity Product: 787.4245536720751

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test