PATENT CLAIM ANALYSIS

Application Number: 15751426
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["514", "04400R"]

Abstract:
There is provided a non-PCR based method for construction of a DNA recombination fragment with necessary flanking regions homologous to a desired site of genetic manipulation within a target DNA required for recombination-based manipulation of said target DNA, comprising the steps of: A) identifying a desired site of insertion for a genetic element in said target DNA; B) locating an endogenous, native, half-site of a selected restriction endonuclease present upstream of the site within the DNA to be targeted for genetic manipulation and thereby defining the 5′ extent of an upstream homology region; C) locating an endogenous, native, half-site of the same selected restriction endonuclease present downstream of the site within the DNA to be targeted for genetic manipulation and thereby defining the 3′ extent of a downstream homology region; D) synthesising a flanking region cassette resulting in juxtapositioning of the upstream and downstream half-sites thereby causing formation of complete restriction site for the selected restriction endonuclease flanked by said upstream and downstream homology regions; and E) inserting the flanking region cassette into a plasmid vector which includes the genetic element to be used to manipulate the target DNA, conferring upon the plasmid vector the necessary flanking homology regions for recombination within the said target DNA at the desired site of insertion.

Claim (Index 4):
The method of  claim 1 , in which the restriction endonuclease site is not present within either the flanking homology region or genetic element used during manipulation of the target DNA.

Metadata:
- Claim Count in Document: 23.0
- Percentile: 88.0
- Lexical Diversity: 2.60227
- Patent Class: 514.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['11910515', '12442727', '15261727', '14577997', '12663172']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7695174951213007
- 35 USC 102 Novelty (BERT): 0.5087210753563061
- Combined Prediction Score: 0.7434378531448014
- Mean Citation Score: 238.353732
- Max Citation Score: 244.29312
- Similarity Product: 156.61030295104982

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test