PATENT CLAIM ANALYSIS

Application Number: 16132211
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-01
Patent Classification: ["506", "004000"]

Abstract:
Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

Claim (Index 1):
A method of detecting a cancer cell comprising:\n (a) isolating each of a plurality of single cells into an individual compartment; (b) obtaining transcripts from each of the single cells; (c) reacting the transcripts from each of the single cells with a set of first probes and a set of second probes,\n i. wherein the set of first probes comprises (1) a first forward probe comprising a sequence complementary to a first subsequence of a first target sequence, and (2) a first reverse probe comprising a sequence complementary to a second subsequence of the first target sequence and a sequence complementary to a non-human, exogenous sequence; \n ii. wherein the set of second probes comprises (1) a second forward probe comprising a sequence complementary to a first subsequence of a second target sequence, and (2) a second reverse probe comprising a sequence complementary to a second subsequence of the second target sequence and the non-human, exogenous sequence; \n (d) performing reverse transcription followed by PCR amplification, thereby generating a fused complex comprising the first target sequence and the second target sequence, wherein the first target sequence is a first gene transcript associated with cancer; and (e) sequencing the fused complex.

Metadata:
- Claim Count in Document: 23.0
- Percentile: 97.0
- Lexical Diversity: 1.71698
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15431660', '15159674', '15920092', '13476911', '15552618']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.58952812744642
- 35 USC 102 Novelty (BERT): 0.5717720638373506
- Combined Prediction Score: 0.587752521085513
- Mean Citation Score: 368.529538
- Max Citation Score: 513.69116
- Similarity Product: 330.8783712322998

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test