PATENT CLAIM ANALYSIS

Application Number: 16158181
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-03
Patent Classification: ["435", "006100"]

Abstract:
Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to simultaneously detect both plant DNA and pathogen DNA in a single assay.

Claim (Index 18):
A method for simultaneously identifying a pathogen and a plant in a single assay comprising the steps of:\n a) harvesting a plant tissue sample comprising one or more pathogens; b) isolating total nucleic acids comprising DNA from at least the plant tissue and the pathogens; c) performing a first amplification in tandem in a single assay in the presence of non-DNA nucleic acids using at least one first primer pair selective for the pathogen DNA and at least one second primer pair selective for the plant DNA, to obtain pathogen-specific first amplicons and plant-specific second amplicons; d) performing a second amplification in tandem using the first amplicons and the second amplicons as template, at least one first fluorescent labeled third primer pair having a sequence complementary to an internal flanking region in the first amplicons and at least one second fluorescent labeled fourth primer pair having a sequence complementary to an internal flanking region in the second amplicons to obtain pathogen-specific first fluorescent labeled third amplicons and plant-specific second fluorescent labeled fourth amplicons; e) hybridizing the third amplicons and the fourth amplicons with nucleic acid probes specific for signature sequence determinants in the pathogen DNA and plant DNA respectively, said nucleic acid probes immobilized at specific known positions on a 3-dimensional lattice microarray via a third fluorescent labeled bifunctional polymer linkers; f) washing the microarray at least once; g) imaging the microarray to detect a first fluorescent signal corresponding to the third amplicons, a second fluorescent signal corresponding to the fourth amplicons and a third fluorescent signal corresponding to the nucleic acid probes immobilized at the specific known positions on the microarray via the third fluorescent labeled bifunctional polymer linkers; h) superimposing each of the first fluorescent signal and the second fluorescent signal with the third fluorescent signal; and j) comparing the sequence of the nucleic acid probe at one or more superimposed signal positions on the microarray with a database of signature sequence determinants for a plurality of pathogen DNA and plant DNA thereby identifying the pathogen and plant in the sample.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 97.0
- Lexical Diversity: 1.71233
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['16158276', '15916036', '12100519', '09865807', '14694008']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6558660479711249
- 35 USC 102 Novelty (BERT): 0.616729497975618
- Combined Prediction Score: 0.6519523929715743
- Mean Citation Score: 492.178766
- Max Citation Score: 703.1072
- Similarity Product: 476.1775266735077

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test