PATENT CLAIM ANALYSIS

Application Number: 15763564
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-11
Patent Classification: ["435", "147000"]

Abstract:
The invention relates to an  Amycolatopsis  strain derived from the  Amycolatopsis  Zyl 926 strain having at least one additional copy of the FCS and ECH genes encoding feruloyl-CoA-synthetase and enoyl-CoA-hydratase/aldolase integrated at the integration site of the φC31 phage. The invention also relates to the method for producing such strains, the integration cassette of the FCS and ECH genes, the use of said strains in a method for producing vanillin, and a method for producing vanillin.

Claim (Index 3):
A process for obtaining an  Amycolatopsis  strain derived from the  Amycolatopsis  Zyl 926 strain filed with the CABI Bioscience under the number IMI 390106 on 2 Mar. 2003, comprising at least one additional copy of the FCS gene encoding feruloyl-coA-synthetase having the sequence SEQ ID NO: 1 or any sequence at least 80%, preferably at least 90%, and more preferably at least 95% identical to SEQ ID NO sequence: 1, and the ECH gene encoding the Enoyl-coA Hydratase/Aldolase having the sequence SEQ ID NO sequence: 2 or any sequence at least 80%, preferably at least 90%, and more preferably at least 95% identical to SEQ ID NO sequence: 2 and of which said additional copies of FCS and ECH genes are specifically integrated at the \u03c6C31 phage integration site, comprising the following steps:\n a. PCR amplification of the FCS gene encoding feruloyl-CoA-synthetase having the sequence SEQ ID NO: 1 or any sequence at least 80%, preferably at least 90%, and more preferably at least 95% identical to SEQ ID NO sequence: 1 and ECH encoding enoyl-CoA-hydratase/Aldolase having sequence SEQ ID NO: 2 or any sequence at least 80%, preferably at least 90%, and more preferably at least 95% identical to SEQ ID NO: 2, isolated from an  Amycolatopsis  strain; b. constructing a vector carrying the nucleic acid sequences obtained in step a, from pSET152 plasmid. c. transformation by electroporation of the ET12567  Escherichia coli  strain with plasmid comprising combining all of the genes encoding the transfer machinery the origin of transfer oriT of which is rendered non-functional, and the vector constructed in step b; d. selection of strains of  Escherichia coli  ET12567 transformed in step c by culture in a medium containing neomycin and a beta-lactam; e. interspecific transfer between  Escherichia coli  ET12567 strains transformed and selected in steps c and d and the  Amycolatopsis  Zyl 926 strain filed with the CABI Bioscience under the number IMI 390106 on 2 Mar. 2003, for implementation in co-culture; f. selection of  Amycolatopsis  Zyl 926 strains having integrated at least one additional copy of the FCS gene encoding feruloyl-coA-synthetase having the sequence SEQ ID NO: 1 or any sequence at least 80%, preferably at least 90%, and more preferably at least 95% identical to SEQ ID NO sequence: 1 and at least one additional copy of the ECH gene encoding enoyl-coA-hydratase/aldolase having the sequence SEQ ID NO sequence: 2 or sequence at least 80/%, preferably at least 90%, and more preferably at least 95% identical to SEQ ID NO: 2 according to step e, by culturing in a medium containing apramycin.

Metadata:
- Claim Count in Document: 29.0
- Percentile: 90.0
- Lexical Diversity: 1.8
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14906429', '13985261', '15879341', '15322745', '14409227']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6277256079605638
- 35 USC 102 Novelty (BERT): 0.5344291886045702
- Combined Prediction Score: 0.6183959660249645
- Mean Citation Score: 331.533256
- Max Citation Score: 386.32785
- Similarity Product: 280.91853527602854

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test