PATENT CLAIM ANALYSIS

Application Number: 15874645
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-05
Patent Classification: ["435", "018000"]

Abstract:
Provided herein are methods of perfusion culturing an adherent mammalian cell using a shake flask and a plurality of microcarriers, and various methods that utilize these culturing methods.

Claim (Index 1):
A method of optimizing a manufacturing process of producing a recombinant protein, the method comprising:\n providing a shake flask containing a mammalian cell disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 20% to about 30% of the volume of the shake flask and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L; incubating the shake flask for a period of time at about 32\u00b0 C. to about 39\u00b0 C. and with a rotary agitation of about 85 revolutions per minute (RPM) to about 125 RPM; and after about the first 48 to 96 hours of the period of time, continuously or periodically removing a first volume of the first liquid culture medium and adding to the first liquid culture medium a second volume of a second liquid culture medium, wherein the first and second volumes are about equal; detecting the recombinant protein in the cell or in the first and/or second culture medium; comparing the amount of recombinant protein present in the cell or in the first and/or second culture medium to a reference level of recombinant protein produced by a different method; and identifying and removing or altering in a manufacturing process any culture components or parameters that are associated with a decrease in the amount of recombinant protein produced as compared to the reference level, or identifying and adding to a manufacturing process any culture components or parameters that are associated with an increase in the amount of recombinant protein produced as compared to the reference level.

Metadata:
- Claim Count in Document: 47.0
- Percentile: 86.0
- Lexical Diversity: 1.25
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14769772', '14769783', '15000522', '14627559', '13847961']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5589968090892776
- 35 USC 102 Novelty (BERT): 0.6340607092009307
- Combined Prediction Score: 0.566503199100443
- Mean Citation Score: 367.944424
- Max Citation Score: 587.53973
- Similarity Product: 557.0211863973939

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test