PATENT CLAIM ANALYSIS

Application Number: 15954805
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "190000"]

Abstract:
It relates to a  Mortierella alpine  ATCC32222 uracil auxotroph strain and a construction method thereof. In the present invention,  Mortierella alpine  ATCC32222 is used as a material and undergoes gene knockout through an  Agrobacterium tumefaciens  mediated genetic manipulation technology, to obtain the  Mortierella alpine  uracil auxotroph. The method is of great significance for the basic theoretic researches of the oil producing fungus  Mortierella alpine  ATCC32222 and product development.

Claim (Index 4):
The method according to  claim 3 , characterized in that the knockout DNA sequence in step (c) is constructed as the following steps:\n designing the primers according to the sequence data of NCBI: P1: (SEQ\u2003ID\u2003NO:\u20035) GACCGGAATTCCGACGCTGACATTACACATTTATCC   P2: (SEQ\u2003ID\u2003NO:\u20036) TGACGGTGGTGCAGGCCAGAGGGCCAAAGATGATGTCGTGCTCAATG   P3: (SEQ\u2003ID\u2003NO:\u20037) TTGAGCACGACATCATCTTTGGCCCTCTGGCCTGCACCACCGTCATT   P4: (SEQ\u2003ID\u2003NO:\u20038) TGCGGGGTACCCATGCGAATCACAGATATGG subsequently, PCR amplifying up- and down-stream DNA fragments by using P1/P2 and P3/P4 with  M. alpina  ATCC 32222 genome DNA as template, then performing fusion PCR by using P1/P4 with up- and down-stream DNA fragments as templates to amplify the KOura5 knockout DNA sequence.

Metadata:
- Claim Count in Document: 11.0
- Percentile: 91.0
- Lexical Diversity: 1.4898
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14910675', '16060417', '10454125', '12512930', '15675916']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6476502329128812
- 35 USC 102 Novelty (BERT): 0.5605214206040265
- Combined Prediction Score: 0.6389373516819957
- Mean Citation Score: 337.012824
- Max Citation Score: 508.22256
- Similarity Product: 313.9217347546005

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test