PATENT CLAIM ANALYSIS

Application Number: 16384782
Application Type: Utility
Filing Date: 2019-04
Publication Date: 2019-08
Patent Classification: ["426", "644000"]

Abstract:
The present invention relates to processes for transfecting cells. In particular, the present invention relates to processes for using CRISPR to incorporate a polynucleotide into the genome of an avian primordial germ cell (PGC).

Claim (Index 10):
A process for using CRISPR to incorporate a polynucleotide into the genome of an avian primordial germ cell (PGC), comprising:\n (i) injecting, into a blood vessel of an avian embryo at stage 13-17 that is contained in an egg, a transfection mixture comprising:\n (a) a DNA polynucleotide encoding a chimeric crRNA-tracrRNA hybrid and a Cas9 nuclease; \n (b) a DNA polynucleotide for integration into the genome of one or more germ cells in the avian embryo; and \n (c) a cationic lipid, \n whereby the DNA polynucleotides enter one or more PGCs in the avian embryo, and \n (1) the chimeric crRNA-tracrRNA hybrid is transcribed; \n (2) the Cas9 nuclease is expressed the chimeric crRNA-tracrRNA hybrid directs the Cas9 nuclease to the target DNA via Watson-Crick base-pairing between the spacer on the chimeric crRNA-tracrRNA hybrid and a protospacer on the target DNA next to a protospacer adjacent motif (PAM); \n (4) the Cas9 nuclease mediates cleavage of target DNA to create a double-stranded break within the protospacer; and \n (5) the DNA polynucleotide of (b) is incorporated into the genome of the one or more PGCs in the avian embryo.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 100.0
- Lexical Diversity: 1.3
- Patent Class: 426.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['16201853', '16201836', '16201855', '14942782', '16201848']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.4326254761605103
- 35 USC 102 Novelty (BERT): 0.5451415581564197
- Combined Prediction Score: 0.4438770843601012
- Mean Citation Score: 371.053074
- Max Citation Score: 373.2707
- Similarity Product: 273.4957942376971

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test