PATENT CLAIM ANALYSIS

Application Number: 16107685
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2019-06
Patent Classification: ["435", "006100"]

Abstract:
The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

Claim (Index 30):
A method for analyzing cellular occupancy of a partition, comprising:\n (a) labelling a first cell with a first cell nucleic acid barcode sequence and a second cell with a second cell nucleic acid barcode sequence to generate a first labelled cell and a second labelled cell, wherein said first cell nucleic acid barcode sequence has a different sequence than said second cell nucleic acid barcode sequence; (b) generating a partition comprising said first labelled cell and said second labelled cell, wherein said partition further comprises a plurality of partition nucleic acid barcode molecules coupled to a bead, wherein each of said plurality of partition nucleic acid barcode molecules comprises a partition nucleic acid barcode sequence; (c) within said partition, using said first cell nucleic acid barcode sequence, said second cell nucleic acid barcode sequence, and said plurality of partition nucleic acid barcode molecules to generate (i) a first barcoded nucleic acid molecule comprising said first cell nucleic acid barcode sequence or a complement thereof and said partition nucleic acid barcode sequence or a complement thereof, and (ii) a second barcoded nucleic acid molecule comprising said second cell nucleic acid barcode sequence or a complement thereof and said partition nucleic acid barcode sequence or a complement thereof, and (d) sequencing said first barcoded nucleic acid molecule and said second barcoded nucleic acid molecule, or a derivative of said first barcoded nucleic acid molecule or said second barcoded nucleic acid molecule, to (i) identify said first cell nucleic acid barcode sequence and said second cell nucleic acid barcode sequence, or a complement of said first cell nucleic acid barcode sequence or said second cell nucleic acid barcode sequence, thereby identifying said first labelled cell and said second labelled cell, and (ii) identify said partition nucleic acid barcode sequence or complement thereof, thereby identifying said first labelled cell and said second labelled cell as originating from said partition based on said first barcoded nucleic acid molecule and said second barcoded nucleic acid molecule having said partition nucleic acid barcode sequence or a complement thereof.

Metadata:
- Claim Count in Document: 72.0
- Percentile: 96.0
- Lexical Diversity: 1.5
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15842687', '15887947', '15875899', '16052431', '15933299']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6133361286294046
- 35 USC 102 Novelty (BERT): 0.5367761395198734
- Combined Prediction Score: 0.6056801297184514
- Mean Citation Score: 376.471042
- Max Citation Score: 394.69577
- Similarity Product: 307.5696531739497

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test