PATENT CLAIM ANALYSIS

Application Number: 16070667
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2019-01
Patent Classification: ["536", "018100"]

Abstract:
The present invention relates to dicaffeoyl spermidine cyclized derivatives and a preparation method and use thereof. Biological activity experiments show that the dicaffeoyl spermidine cyclized derivatives of the present invention have anti-Senile dementia activity and antioxidant activity, and their activity are even better than that of a positive control drug, thus suitable for the prevention and treatment of neurodegenerative diseases such as Senile dementia.

Claim (Index 10):
A method for preparing the dicaffeoyl spermidine cyclized derivatives or the pharmaceutically acceptable salts thereof according to  claim 1 , comprising the following steps:\n (1) The fructus lycii was extracted 3 times for 2 hours each time by heating under reflux with ethanol-water having the volume ratio of 60:40, after filtration, the filtrate was concentrated under reduced pressure to obtain a concentrated solution; (2) The concentrated solution was chromatographed through a macroporous resin column and eluted successively with ethanol-water having the volume ratios of 0:100, 30:70, 50:50, 70:30, and 95:5 to obtain 5 fractions of F1, F2, F3, F4, F5; (3) The fraction F2, obtained by the elution with ethanol-water having the volume ratio of 30:70, was subjected to a silica gel column chromatography under normal pressure, and eluted successively with chloroform-methanol-water having the volume ratios of 95:5:0, 90:10:1, 85:15:1.5, 80:20:2, 70:30:3, 60:40:4, 50:50:5, 40:60:6 and 0:100:0 to obtain a total of 10 sub-fractions of F2.1, F2.2, F2.3, F2.4, F2.5, F2.6, F2.7, F2.8, F2.9 and F2.10; (4) The sub-fraction F2.9, obtained by the elution with chloroform-methanol-water having the volume ratio of 40:60:6, was subjected to a low-medium pressure liquid phase ODS column chromatography, and eluted successively with methanol-water-trifluoroacetic acid having the volume ratios of 5:95:0.1, 10:90:0.1, 15:85:0.1, 20:80:0.1, 25:75:0.1, 30:70:0.1, 40:60:0.1 and 100:0:0 to obtain a total of 9 sub-fractions of F2.9.1, F2.9.2, F2.9.3, F2.9.4, F2.9.5, F2.9.6, F2.9.7, F2.9.8 and F2.9.9; (5) The sub-fraction F2.9.6, obtained by the elution with methanol-water-trifluoroacetic acid having the volume ratio of 15:85:0.1, was subjected to a reversed phase preparative HPLC, and eluted with methanol-water-trifluoroacetic acid having the volume ratio of 20:80:0.1 at a flow rate of 8 mL/min, to obtain the dicaffeoyl spermidine cyclized derivatives or the pharmaceutically acceptable salts thereof.

Metadata:
- Claim Count in Document: 23.0
- Percentile: 95.0
- Lexical Diversity: 1.47826
- Patent Class: 536.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15170871', '13934355', '13934277', '15527280', '13542687']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.596855913869795
- 35 USC 102 Novelty (BERT): 0.5042470033496965
- Combined Prediction Score: 0.5875950228177851
- Mean Citation Score: 196.039112
- Max Citation Score: 233.38445
- Similarity Product: 138.40606137644647

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test