PATENT CLAIM ANALYSIS

Application Number: 15921442
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-09
Patent Classification: ["435", "123000"]

Abstract:
Methods of producing peptide beta-lactones and beta-hydroxy acids are disclosed that include contacting a beta-hydroxy-alpha-amino acid, an aryl carrier protein (ObiD), and ATP with a non-ribosomal protein synthetase. A continuous flow reactor is disclosed that includes an elongate conduit with at least one region that includes a first region with a non-ribosomal protein synthetase immobilized to a substrate. The non-ribosomal protein synthetase of the continuous flow reactor is configured to contact a flow of a reaction mixture that includes a beta-hydroxy-alpha-amino acid and an aryl carrier protein. The non-ribosomal protein synthetase is further configured to release a peptide beta-lactone into the flow of the reaction mixture.

Claim (Index 17):
The continuous flow reactor of  claim 15 , wherein the at least one region further comprises a second region positioned upstream of the first region, the second region comprising a serine hydroxymethyltransferase/threonine aldoloase immobilized to a second substrate, the serine hydroxymethyltransferase/threonine aldoloase selected from the group consisting of ObiH, a homolog of ObiH, and any variation thereof comprising the amino acid sequence of SEQ ID NO:3 or fragment thereof, wherein the immobilized serine hydroxymethyltransferase/threonine aldoloase is configured to contact an aliphatic or aryl aldehyde selected from the group consisting of aliphatic aldehydes, aromatic benzaldehydes, aromatic phenylacetaldehydes, and aromatic cinnamaldehydes; a pyridoxyl phosphate (PLP) cofactor and an amino acid selected from the group consisting of threonine, serine, glycine, and any isomer thereof, and to release the beta-hydroxy-alpha-amino acid into the flow of the reaction mixture.

Metadata:
- Claim Count in Document: 11.0
- Percentile: 90.0
- Lexical Diversity: 2.07273
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12842549', '12118362', '10925357', '11775216', '10017324']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5326520906094013
- 35 USC 102 Novelty (BERT): 0.5062770273329891
- Combined Prediction Score: 0.5300145842817601
- Mean Citation Score: 208.44705400000004
- Max Citation Score: 260.4539
- Similarity Product: 189.0752208867252

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test