PATENT CLAIM ANALYSIS

Application Number: 15949178
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["435", "283100"]

Abstract:
Devices and methods generate an ordered restriction map of genomic DNA extracted from whole cells, nuclei, whole chromosomes, or other sources of long DNA molecules. The devices have a fluidic microchannel that merges into a reaction nanochannel that merges into a detection nanochannel at an interface where the nanochannel diameter decreases in size by between 50% to 99%. Intact molecules of DNA are transported to the reaction nanochannel and then fragmented in the reaction nanochannel using restriction endonuclease enzymes. The reaction nanochannel is sized and configured so that the fragments stay in an original order until they are injected into the detection nanochannel. Signal at one or more locations along the detection nanochannel is detected to map fragments in the order they occur along a long DNA molecule.

Claim (Index 1):
A nanofluidic analysis system, comprising:\n a first reaction nanochannel that is from 500 \u03bcm to 10 cm long and that merges into a first detection nanochannel at an interface position therebetween where the first detection nanochannel reduces in size relative to the first reaction nanochannel; a microfluidic channel in communication with an ingress portion of the first reaction nanochannel; a first electrode in communication with the microfluidic channel; a first transverse fluidic channel extending from and in fluid communication with the first reaction nanochannel at a location that is spaced apart from but proximate the ingress portion of the first reaction nanochannel; a second electrode in communication with the first transverse fluidic channel; a second transverse fluidic channel extending from and in fluid communication with the first reaction nanochannel downstream of the first transverse fluidic channel; a third electrode in communication with the second transverse fluidic channel; a fourth electrode in communication with the first detection nanochannel; a circuit configured to control operation of the first, second, third and fourth electrodes to controllably thread, load and digest DNA within the first reaction nanochannel to thereby prevent fragment disordering; at least one electrical or optical detector in communication with the first detection nanochannel configured to spatially and temporally resolve fragment size to thereby allow an ordered restriction map of chromosomal DNA in real time or near real time; and a second reaction nanochannel that is from 500 \u03bcm to 10 cm long that resides downstream of the first reaction nanochannel and that merges into a second detection nanochannel at a second interface position therebetween where the second detection nanochannel reduces in size relative to the second reaction nanochannel.

Metadata:
- Claim Count in Document: 29.0
- Percentile: 91.0
- Lexical Diversity: 1.79221
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14771989', '14204211', '15446630', '14926595', '15568980']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6305172398519671
- 35 USC 102 Novelty (BERT): 0.5850364097035051
- Combined Prediction Score: 0.625969156837121
- Mean Citation Score: 462.629984
- Max Citation Score: 498.09015
- Similarity Product: 431.8550773377478

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test