PATENT CLAIM ANALYSIS

Application Number: 15871218
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-08
Patent Classification: ["514", "019300"]

Abstract:
The present invention discloses a polypeptide compound, a preparation method and an application thereof. The structural formula of the polypeptide compound is (X A X B X C X D X E X F X G —X) 2 KY or {(X A X B X C X D X E X F X G —X) 2 K} 2 KY or {({X A X B X C X D X E X F X G —X} 2 K) 2 K} 2 KY, where, X A , X B , X D , X E  and X G  are one of aliphatic amino acid molecules respectively, X C  and X F  are aliphatic amino acid molecules or heterocyclic amino acid molecules, K is lysine (Lys, K), X or Y is null or any one or more amino acid or chemical groups. The polypeptide compound provided in the invention has an effect of enhancing the immune function of a body and has an application potential of being developed into a clinical medicine capable of enhancing the immune function of a body.

Claim (Index 21):
The method according to  claim 16 , comprising the following steps:\n step 1: protecting the two amino groups of the lysine K with an Fmoc protection group/method; step 2: fixing KY to the WANG solid resin with an automatic polypeptide synthesizer, in the following bonding sequence: KY-WANG solid resin; when the two-copy polypeptide compound is prepared, the two activated terminal amino groups of lysine in KY are further condensed with another two X A X B X C X D X E X F X G \u2014X fragments, to obtain the polypeptide compound (X A X B X C X D X E X F X G \u2014X) 2 KY with two copies of X A X B X C X D X E X F X G \u2014X, which is fixed to the WANG solid resin; or the two activated terminal amino groups of the lysine in KY are further condensed with another two lysines K, in each of which the two amino groups have been protected with an Fmoc protection method, to obtain a two-branch skeleton K 2 KY-WANG solid resin; when the four-copy polypeptide compound is prepared, the two activated terminal amino groups of each lysine in the two-branch skeleton \u201cK 2 \u201d are further condensed with two X A X B X C X D X E X F X G \u2014X fragments, to obtain the polypeptide compound {(X A X B X C X D X E X F X G \u2014X) 2 K} 2 KY with four copies of X A X B X C X D X E X F X G \u2014X, which is fixed to the WANG solid resin; or the two activated terminal amino groups of each lysine in the two-branch skeleton \u201cK 2 \u201d are further condensed with another two lysines K, in each of which the two amido groups have been protected with an Fmoc protection method, to obtain a four-branch skeleton K 4 K 2 KY-WANG solid resin; or when the eight-copy polypeptide compound is prepared, the two activated terminal amino groups of each lysine in the four-branch skeleton \u201cK 4 \u201d are further condensed with two X A X B X C X D X E X F X G \u2014X fragments, to obtain the polypeptide compound {({X A X B X C X D X E X F X G \u2014X} 2 K)  2 K} 2 KY with eight copies of X A X B X C X D X E X F X G \u2014X, which is fixed to the WANG solid resin; where, the polypeptide compound is cleaved from the WANG solid resin with a TFA method, to obtain a crude polypeptide compound product; step 3: purifying the crude polypeptide compound product with a chromatographic column (model: Daiso C18, 10 \u03bcm, 100 \u212b, 50\u00d7250 mm), wherein, the mobile phase A is an aqueous solution that contains 0.05% trifluoroacetic acid and 2% acetonitrile, the mobile phase B is 90% acetonitrile/water, the flow rate is 25 mL/min., and the ultraviolet detection wavelength is 220 nm; the eluting peak solution is collected and then freeze-dried, to obtain a white flocculent polypeptide compound.

Metadata:
- Claim Count in Document: 29.0
- Percentile: 86.0
- Lexical Diversity: 2.92754
- Patent Class: 514.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15852288', '13168453', '11721910', '15320112', '15129846']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7378350359037228
- 35 USC 102 Novelty (BERT): 0.5535711883827557
- Combined Prediction Score: 0.7194086511516261
- Mean Citation Score: 241.150632
- Max Citation Score: 417.65292
- Similarity Product: 358.44938304831504

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test