PATENT CLAIM ANALYSIS

Application Number: 15991370
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2019-08
Patent Classification: ["435", "100000"]

Abstract:
The present invention discloses a maltooligosyl trehalose trehalohydrolase (MTHase) mutant and application thereof, belonging to the technical fields of gene engineering and enzyme engineering. The present invention provides a series of MTHase mutants to prepare trehalose, having a better effect. Further, the MTHase mutant is expressed in  Escherichia coli  BL21 (DE3) and the enzyme is optimized by fermentation, which can significantly increase the yield of enzymes.

Claim (Index 1):
A mutant of a maltooligosyl trehalose trehalohydrolase, comprising an amino acid sequence with mutations selected from the group consisting of mutation of methionine (Met) at position 140, mutation of leucine (Leu) at position 202, mutation of leucine (Leu) at position 218, mutation of tyrosine (Tyr) at position 323, mutation of phenylalanine (Phe) at position 338 and mutation of isoleucine (Ile) at position 404; wherein the mutations are relative to a parent amino acid sequence of a maltooligosyl trehalose trehalohydrolase derived from  Sulfolobus acidocaldarius  set forth in SEQ ID NO.2.

Metadata:
- Claim Count in Document: 3.0
- Percentile: 93.0
- Lexical Diversity: 1.54
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['13210646', '11418804', '12189232', '15820487', '11611058']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5687804787131218
- 35 USC 102 Novelty (BERT): 0.5080715353299586
- Combined Prediction Score: 0.5627095843748056
- Mean Citation Score: 263.514026
- Max Citation Score: 267.1345
- Similarity Product: 190.74714953792096

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test