PATENT CLAIM ANALYSIS

Application Number: 15983209
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2018-09
Patent Classification: ["506", "009000"]

Abstract:
The present invention generally relates to a set of early developmental reference data or “lineage scorecard” for stem cells, and methods, systems and kits to generate a lineage scorecard for predicting the functionality and suitability of stem cell lines. In some aspects, methods for generating a scorecard comprises measuring the gene expression of a plurality of early developmental genes, such as pluripotent, early ectoderm, early mesoderm and early endoderm genes to predict the pluripotency and differentiation potential of the stem cell line and its functionality and/or suitability for a desired use. In some embodiments, a reference scorecard can be compared with the test stem cell line scorecard to accurately predict the utility and/or identify specific characteristics of the stem cell line, e.g., to determine its suitability for downstream applications, e.g., therapeutic use, drug screening, toxicity assays, differentiation into a desired cell lineage, etc.

Claim (Index 9):
An array composition for characterizing the differentiation potential of a pluripotent stem cell, comprising at least 12 oligonucleotides, or at least 12 pairs of oligonucleotides, that amplify the mRNA of a set of at least 12 early developmental genes selected from the group of at least 4 ectoderm genes selected from the group consisting of SEQ ID NO: 11, 14, 15, 19, 20, 21 and 22;\n at least 4 endoderm genes selected from the group consisting of SEC ID NO: 23, 30, 32, 33, 34, 36, 37, 44, 45, 46, 48 and 49; and at least 4 mesoderm genes selected from the group consisting of SEQ ID NO: SEQ ID NO: 53, 62, 63, 65, 66, 68 and 70; wherein the oligonucleotides or pair of oligonucleotides are immobilized on a solid support.

Metadata:
- Claim Count in Document: 30.0
- Percentile: 93.0
- Lexical Diversity: 2.01235
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14936774', '13114102', '12664763', '14439037', '13998974']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5762039096458351
- 35 USC 102 Novelty (BERT): 0.5339338584278656
- Combined Prediction Score: 0.5719769045240382
- Mean Citation Score: 351.49161200000015
- Max Citation Score: 374.56046
- Similarity Product: 238.891974178462

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test