PATENT CLAIM ANALYSIS

Application Number: 16198902
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-03
Patent Classification: ["435", "006120"]

Abstract:
The present invention provides methods, compositions and kits for enriching and determining nucleotide sequences of a plurality of target loci from a sample comprising nucleic acids. The methods comprise one or more cycles of primer extension followed by PCR amplification of target sequences using nested target-specific primers.

Claim (Index 2):
The method according to  claim 1 , characterized in that:\n (1) the amplicons of the target nucleotide sequence are used for next-generation sequencing (NGS); (2) the method comprising enriching a target nucleotide sequence having the locus of interest using a first set of outside primer and inside primer that can specifically anneal to the first strand of the ligated nucleic acid, and a second set of outside primer and inside primer that can specifically anneal to the second strand of the ligated nucleic acid, optionally, the outside primer of the first set and the outside primer of the second set comprises a first 5\u2032 tag sequence of at least about 13 nucleotides long, and wherein the inside primer of the first set and the inside primer of the second set comprises a second 5\u2032 tag sequence of at least about 13 nucleotides long, for example, the GC content of the first 5\u2032 tag sequence is substantially similar to the GC content of the nucleic acid template; (3) the method comprising enriching a plurality of target nucleotide sequences having different loci of interest using a plurality of sets of outside primer and inside primer, optionally, wherein: (a) at least 2 of the plurality of target nucleotide sequences are present in different strands of the nucleic acid template; and/or (b) the method comprising enriching a plurality of target nucleotide sequences having about 2-5000 different loci of interest; and/or (4) steps (c)-(e) are repeated for about 2-100 cycles; (5) the outside primer anneals to a region about 1-100 nucleotides farther away from the locus of interest than the inside primer; and/or (6) at least the last 12 nucleotides of the outside primer and/or the inside primer have fewer than about 20 different annealing loci in the nucleic acid sample.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 98.0
- Lexical Diversity: 1.25
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14463498', '13793564', '15269448', '12069174', '14300048']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5627469024840308
- 35 USC 102 Novelty (BERT): 0.5328082845675631
- Combined Prediction Score: 0.5597530406923841
- Mean Citation Score: 339.0472660000001
- Max Citation Score: 356.35602
- Similarity Product: 246.2827011305952

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test