PATENT CLAIM ANALYSIS

Application Number: 16147353
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-04
Patent Classification: ["435", "173600"]

Abstract:
The present disclosure provides methods and devices for rapid and efficient modification of a variety of cell types, including mammalian cells, plant cells, archaea, yeasts, and bacteria, by novel methods of introducing exogenous materials, e.g. nucleic acids.

Claim (Index 1):
A method of electroporating cells comprising:\n providing a flow-through electroporation (FTEP) device, wherein the flow-through electroporation device comprises an inlet and at least an inlet channel for receiving a fluid comprising electrocompetent cells and exogenous material into the FTEP device; an outlet and an outlet channel for removing a fluid comprising transformed cells and exogenous material from the FTEP device; a flow channel intersecting and positioned between the inlet channel and the outlet channel, wherein the flow channel decreases in width to a dimension no smaller than at least 2\u00d7 diameter of the cells being electroporated between the first inlet channel and a center of the flow channel and the outlet channel and the center of the flow channel; and two electrodes positioned between the intersection of the flow channel with the inlet channel and the intersection of the flow channel with the outlet channel; wherein the electrodes are in fluid and electrical communication with fluid in the flow channel but not directly in the path of the cells traveling through the flow channel; flowing the cells comprising the electrocompetent cells and exogenous material into the inlet and the inlet channel; flowing the cells through the flow channel and past the two electrodes; providing electrical pulses to the cells in the fluid as the cells flow through the flow channel past the electrodes producing electroporated cells; and removing the electroporated cells from the outlet channel and outlet.

Metadata:
- Claim Count in Document: 48.0
- Percentile: 97.0
- Lexical Diversity: 1.39394
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['16147120', '16024816', '10225446', '13518514', '14209046']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5691813410538487
- 35 USC 102 Novelty (BERT): 0.5616646614062005
- Combined Prediction Score: 0.5684296730890839
- Mean Citation Score: 225.414766
- Max Citation Score: 450.7326
- Similarity Product: 368.1535510700941

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test