PATENT CLAIM ANALYSIS

Application Number: 15979144
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2019-05
Patent Classification: ["435", "157000"]

Abstract:
The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.

Claim (Index 2):
A method of constructing the genetically modified yeast cell of the present invention comprising at least six or more of the following steps: (a) introducing a first nucleic acid encoding MmFAR1, or functional fragment thereof, operatively linked to a promoter capable of expressing the MmFAR1 gene product in the yeast cell; (b) introducing a second nucleic acid encoding ACC1, or functional fragment thereof, operatively linked to a promoter capable of expressing the ACC1 gene product in the yeast cell, or replacing the native promoter of ACC1 with a promoter with a higher transcription activity, such as promoter TEF1; (c) introducing a third nucleic acid encoding FAS1, or functional fragment thereof, operatively linked to a promoter capable of expressing the FAS1 gene product in the yeast cell, or replacing the native promoter of FAS1 with a promoter with a higher transcription activity, such as promoter TEF1; (d) introducing a fourth nucleic acid encoding FAS2, or functional fragment thereof, operatively linked to a promoter capable of expressing the FAS2 gene product in the yeast cell, or replacing the native promoter of FAS2 with a promoter with a higher transcription activity, such as promoter TEF1; (e) removing all or a portion of the DGA1 gene, or introducing a nucleic acid into the yeast cell targeting the DGA1 gene or its gene product, such that the yeast cell has a reduced amount of activity of the DGA1 gene product; (f) removing all or a portion of the HFD1 gene, or introducing a nucleic acid into the yeast cell targeting the HFD1 gene or its gene product, such that the yeast cell has a reduced amount of activity of the HFD1 gene product; (g) removing all or a portion of the ADH6 gene, or introducing a nucleic acid into the yeast cell targeting the ADH6 gene or its gene product, such that the yeast cell has a reduced amount of activity of the ADH6 gene product; (h) introducing a fifth nucleic acid encoding a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, or functional fragment thereof, operatively linked to a promoter capable of expressing the mutant ACC1 gene product in the yeast cell; (i) removing all or a portion of the GDH1 gene, or introducing a nucleic acid into the yeast cell targeting the GDH1 gene or its gene product, such that the yeast cell has a reduced amount of activity of the GDH1 gene product; (j) introducing a sixth nucleic acid encoding OLE1, or functional fragment thereof, operatively linked to a promoter capable of expressing the OLE1 gene product in the yeast cell; and (k) introducing a seventh nucleic acid encoding a second copy of MmFAR1, or functional fragment thereof, operatively linked to a promoter capable of expressing the MmFAR1 gene product in the yeast cell.

Metadata:
- Claim Count in Document: 5.0
- Percentile: 93.0
- Lexical Diversity: 1.8
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15005719', '13656086', '14547304', '13704776', '15562951']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5944463605652688
- 35 USC 102 Novelty (BERT): 0.5420554364420677
- Combined Prediction Score: 0.5892072681529488
- Mean Citation Score: 363.167662
- Max Citation Score: 387.4936
- Similarity Product: 308.1344912151336

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test