PATENT CLAIM ANALYSIS

Application Number: 15920092
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-09
Patent Classification: ["506", "004000"]

Abstract:
Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

Claim (Index 30):
A method for functional analysis of biological cells, comprising:\n isolating into a monodisperse emulsion microdroplet a target cell from a plurality of target cell clones of a first cell type and one or more inducer cells from a plurality of inducer cell clones of a second cell type; incubating isolated cells in the monodisperse emulsion microdroplet, wherein the isolated cells comprise the single target cell and the one or more inducer cells; isolating RNA from the isolated cells; generating a library of hybridized polynucleic acids using the composition of  claim 24 ; and deep sequencing the library of hybridized polynucleic acids.

Metadata:
- Claim Count in Document: 53.0
- Percentile: 90.0
- Lexical Diversity: 1.525
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15159674', '15431660', '15159705', '14734953', '15156217']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5517068603656418
- 35 USC 102 Novelty (BERT): 0.5168154939406093
- Combined Prediction Score: 0.5482177237231385
- Mean Citation Score: 267.570496
- Max Citation Score: 299.69458
- Similarity Product: 234.9159044657969

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test