PATENT CLAIM ANALYSIS

Application Number: 16128360
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-03
Patent Classification: ["800", "003000"]

Abstract:
A genetically modified mouse is provided, wherein the mouse expresses an immunoglobulin light chain repertoire characterized by a limited number of light chain variable domains. Mice are provided that present a choice of two human light chain variable gene segments such that the immunoglobulin light chains expresses by the mouse comprise one of the two human light chain variable gene segments. Methods for making bispecific antibodies having universal light chains using mice as described herein, including human light chain variable regions, are provided. Methods for making human variable regions suitable for use in multispecific binding proteins, e.g., bispecific antibodies, and host cells are provided.

Claim (Index 38):
A method of making a fully human immunoglobulin heavy chain or a fully human immunoglobulin light chain comprising the steps of:\n (a) immunizing a genetically modified mouse with an antigen of interest, wherein the genetically modified mouse comprises in its germline genome:\n (i) exactly two unrearranged human immunoglobulin V\u03ba gene segments and five unrearranged human immunoglobulin J\u03ba gene segments operably linked to a mouse immunoglobulin light chain constant region sequence at the endogenous kappa light chain loci of the mouse, wherein the two unrearranged human immunoglobulin V\u03ba gene segments are a human V\u03ba1-39 gene segment and a human V\u03ba3-20 gene segment; and \n (ii) one or more unrearranged human immunoglobulin V H  gene segments, one or more unrearranged human immunoglobulin D H  gene segments, and one or more unrearranged human immunoglobulin J H  gene segments operably linked to a mouse immunoglobulin heavy chain constant region sequence at the endogenous heavy chain loci of the mouse;\n wherein the unrearranged human immunoglobulin heavy chain and kappa light chain gene segments of the genetically modified mouse are capable of rearranging and encoding human immunoglobulin variable domains of an antibody, wherein the genetically modified mouse does not comprise endogenous immunoglobulin V\u03ba or J\u03ba gene segments that are capable of rearranging to form an immunoglobulin light chain variable region sequence; \n \n (b) determining a human immunoglobulin heavy or light chain variable domain sequence of an antibody that specifically binds the antigen and that was generated by the genetically modified mouse; and (c) operably linking the human immunoglobulin heavy or light chain variable domain sequence to a human immunoglobulin heavy or light chain constant domain sequence, respectively, to form a fully human immunoglobulin heavy chain or a fully human immunoglobulin light chain.

Metadata:
- Claim Count in Document: 44.0
- Percentile: 97.0
- Lexical Diversity: 1.86885
- Patent Class: 800.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15700973', '14473970', '13798455', '14705916', '13566765']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7866122052929573
- 35 USC 102 Novelty (BERT): 0.720372674064501
- Combined Prediction Score: 0.7799882521701117
- Mean Citation Score: 885.906042
- Max Citation Score: 940.1538
- Similarity Product: 786.1464076530217

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test