PATENT CLAIM ANALYSIS

Application Number: 16170208
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-02
Patent Classification: ["506", "009000"]

Abstract:
A method of using an exchange-induced remnant magnetization (EXIRM) technique for label free detection of short strands of nucleotides and cancer biomarkers, such as DNA and microRNA strands, DNA/RNA-binding biomarkers, and cancer-specific antigens, with high sensitivity, high specificity, and broad dynamic range. The method may provide a label-free approach aimed to facilitate high reliability, and to require a minimum amount of biochemical reagents.

Claim (Index 1):
A method of detecting nucleotide sequences, comprising\n a) immobilizing a first nucleotide single strand on a surface; b) adding a second nucleotide single strand to the first nucleotide single strand to form a hybridized double strand, wherein said second strand comprises:\n a first magnetic particle; and \n a nucleotide sequence that comprises at least a first mismatched base; \n c) measuring a first magnetic signal value for said hybridized double strand; d) incubating a third nucleotide strand with said hybridized double strand; wherein said third strand is complementary to said first strand, and wherein said incubating forms an exchange product; e) measuring a second magnetic signal value for the exchange product of step d after applying a weak mechanical force to remove nonspecifically bound magnetic particles; and f) quantifying the amount of said third nucleotide strand from the difference in magnetic signal values measured in step c and step e, wherein the difference in the magnetic signal values comprises a decrease in magnetic signal values due to the randomization of the second nucleotide single strand magnetic dipoles.

Metadata:
- Claim Count in Document: 32.0
- Percentile: 97.0
- Lexical Diversity: 1.39623
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12835869', '11958180', '15450675', '15209090', '14571142']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6235308881965648
- 35 USC 102 Novelty (BERT): 0.5088485049415858
- Combined Prediction Score: 0.6120626498710668
- Mean Citation Score: 276.811358
- Max Citation Score: 279.44
- Similarity Product: 192.9974307441712

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test