PATENT CLAIM ANALYSIS

Application Number: 16007424
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-10
Patent Classification: ["435", "194000"]

Abstract:
A DNA polymerase, having an amino acid sequence represented by SEQ ID NO:2, or a derivative of the amino acid sequence by substitution, deletion, or addition of at least one amino acid residue. The DNA polymerase is a hybrid DNA polymerase prepared by inserting a thioredoxin binding domain (TBD) of bacteriophage T7 DNA polymerase into a DNA polymerase I (Sau) of  Staphylococcus aureus . A method for preparing the DNA polymerase includes: 1) determining a corresponding position and a target substitution sequence in Sau protein for the TBD of the bacteriophage T7 DNA polymerase; 2) devising and synthesizing a primer according to a gene sequence of Sau and a sequence TBD published by GenBank; 3) cloning the Sau-TBD segment acquired in (2) to an expression vector pTrc99A to construct a recombinant vector pTrc99A-Sau-TBD; and 4) transforming  Escherichia coli  by the recombinant vector pTrc99A-Sau-TBD and inducing protein expression.

Claim (Index 2):
A polynucleotide sequence of  claim 1 , wherein the DNA polymerase has the amino acid sequence of SEQ ID NO:2.

Metadata:
- Claim Count in Document: 9.0
- Percentile: 94.0
- Lexical Diversity: 1.9881
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['15256732', '15119867', '13375264', '14135860', '14555863']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5019757331750007
- 35 USC 102 Novelty (BERT): 0.6238408312019017
- Combined Prediction Score: 0.5141622429776908
- Mean Citation Score: 270.562496
- Max Citation Score: 591.5704
- Similarity Product: 500.0930097895623

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test