PATENT CLAIM ANALYSIS

Application Number: 16158181
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-03
Patent Classification: ["435", "006100"]

Abstract:
Provided herein is a dual amplification method for detecting plant pathogens by analysis of pathogen DNA in an unpurified nucleic acid sample from the plant. Pathogen-specific primers are used to generate a first set of amplicons that are further amplified in a second amplification step using fluorescent tagged pathogen-specific primers. Fluorescent amplicons thus generated are hybridized with pathogen-specific nucleic acid probes that are immobilized on a solid support using bifunctional polymer linkers. The hybridized microarray is imaged to obtain fluorescent images of the amplicons and the nucleic acid probes, which are superimposed to detect the pathogen present in the plant. Also described herein is a method to simultaneously detect both plant DNA and pathogen DNA in a single assay.

Claim (Index 7):
The method of  claim 1 , wherein the pathogen is a bacterium, said nucleic acid probes have sequences shown in SEQ ID NOS: 37-85.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 97.0
- Lexical Diversity: 1.71233
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['16158276', '15916036', '12100519', '09865807', '14694008']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6579993956032145
- 35 USC 102 Novelty (BERT): 0.6870841330390778
- Combined Prediction Score: 0.6609078693468008
- Mean Citation Score: 492.178766
- Max Citation Score: 703.1072
- Similarity Product: 682.2418184768676

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test