PATENT CLAIM ANALYSIS

Application Number: 16069824
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2019-01
Patent Classification: ["506", "016000"]

Abstract:
The present invention relates to the field of microfluidics and in particular to analysing the gene expression of a cell in response to a ligand expressed in the same microfluidic compartment. By barcoding the transcriptome of the cell and of the expression system generating the ligand, the effect of the ligand on the cell expression can be discerned. The invention provides microfluidic compartments and methods for this purpose.

Claim (Index 12):
A method for determining the gene expression of a cell, comprising the steps:\n (a) providing a plurality of microfluidic compartments according to  claim 1 , (b) lysing the cells comprised in the compartments, (c) reverse transcribing mRNA released from the cells to cDNA, (d) amplifying the cDNA, (e) determining the sequence and optionally the respective amount of the cDNA, and (f) selecting sequences comprising the same barcode sequence, wherein the following sequences are excluded:\n sequences derived from a second cell or a cell-free expression system, \n sequences comprising a unique barcode sequence that is not associated in other sequences with a nucleotide sequence encoding for a polypeptide ligand expressed by a second cell or a cell-free expression system, \n sequences comprising a unique barcode sequence that is associated with a nucleotide sequence encoding for more than one polypeptide ligand expressed by a second cell or a cell-free expression system, \n wherein step (c) or step (d) is carried out with a barcode oligonucleotides as a primer.

Metadata:
- Claim Count in Document: 35.0
- Percentile: 95.0
- Lexical Diversity: 1.7561
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15847752', '15980473', '14407849', '13899397', '14586857']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5613541734961106
- 35 USC 102 Novelty (BERT): 0.4971600065414432
- Combined Prediction Score: 0.554934756800644
- Mean Citation Score: 272.0206380000001
- Max Citation Score: 275.86694
- Similarity Product: 231.05703754970315

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test