PATENT CLAIM ANALYSIS

Application Number: 15917529
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-07
Patent Classification: ["530", "364000"]

Abstract:
A method for separating and purifying recombinant human serum albumin (rHSA) from transgenic rice grain, sequentially comprising the steps of: 1) subjecting crude extract of rHSA to cation exchange chromatography to obtain primary product I; 2) subjecting the primary product I to anion exchange chromatography to obtain secondary product II; 3) subjecting the secondary product II to hydrophobic chromatography to obtain purified rHSA. The method may further comprise a step of ceramic hydroxyapatite chromatography prior to the hydrophobic chromatography. The method has the advantages of low cost and easy operation. The resultant rHSA has a purity of about 99% by HPLC.

Claim (Index 10):
The method according to  claim 1 , wherein said crude extract of the recombinant human serum albumin is prepared by a method comprising the steps of:\n i) mixing milled transgenic rice grain containing recombinant human serum albumin with an extraction buffer in a w/v (kg/L) ratio of 1:5 and extracting at 55\u02dc60\u00b0 C. for 1\u02dc1.5 hours to obtain mixture I; said extraction buffer comprising 10\u02dc30 mM phosphate buffer, 10\u02dc20 mM sodium acetate, 15\u02dc30 mM ammonia sulfate and 5\u02dc20 mM sodium caprylate, with a pH of 6.5\u02dc8; ii) adjusting the pH of mixture I of step i) to 4.0\u02dc4.5 and precipitating it for 3\u02dc12 hours to obtain mixture II; iii) filtrating the mixture II of step ii) and collecting the filtrate to obtain a crude extract containing high concentration of recombinant human serum albumin; said filtrating comprising steps of filtrating by pressure filtration with a filter cloth type plate-frame filter, then filtrating by micro-filtration with a polyethersulfone hollow fiber membrane with a pore size of 0.20 \u03bcm\u02dc0.45 \u03bcm.

Metadata:
- Claim Count in Document: 59.0
- Percentile: 90.0
- Lexical Diversity: 1.71642
- Patent Class: 530.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['13206884', '14676727', '15707888', '15033335', '14596978']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7346647790723445
- 35 USC 102 Novelty (BERT): 0.6918264883028321
- Combined Prediction Score: 0.7303809499953933
- Mean Citation Score: 524.856532
- Max Citation Score: 812.2995599999997
- Similarity Product: 695.4366400030801

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test