PATENT CLAIM ANALYSIS

Application Number: 15983209
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2018-09
Patent Classification: ["506", "009000"]

Abstract:
The present invention generally relates to a set of early developmental reference data or “lineage scorecard” for stem cells, and methods, systems and kits to generate a lineage scorecard for predicting the functionality and suitability of stem cell lines. In some aspects, methods for generating a scorecard comprises measuring the gene expression of a plurality of early developmental genes, such as pluripotent, early ectoderm, early mesoderm and early endoderm genes to predict the pluripotency and differentiation potential of the stem cell line and its functionality and/or suitability for a desired use. In some embodiments, a reference scorecard can be compared with the test stem cell line scorecard to accurately predict the utility and/or identify specific characteristics of the stem cell line, e.g., to determine its suitability for downstream applications, e.g., therapeutic use, drug screening, toxicity assays, differentiation into a desired cell lineage, etc.

Claim (Index 13):
A method of determining the differentiation potential of a pluripotent stem cell line comprising; amplifying the mRNA, to produce cDNA, of a set of at least 12 early developmental genes selected from the group consisting of:\n at least 4 ectoderm genes selected from the group consisting of SEQ ID NO: 11, 14, 15, 19, 20, 21 and 22; at least 4 endoderm genes selected from the group consisting of SEQ ID NO: 23, 30, 32, 33, 34, 36, 37, 44, 45, 46, 48 and 49; and at least 4 mesoderm genes selected from the group consisting of SEQ ID NO: 53, 62, 63, 65, 66, 68 and 70; in said pluripotent stem cell line using an array having a solid support comprising oligonucleotides, or pairs of oligonucleotides immobilized or embedded within the solid support that hybridize to the at least 12 early developmental genes, wherein the amount of cDNA produced corresponds to the amount of mRNA for each early developmental gene amplified; measuring the level of cDNA for each of the at least 12 early developmental genes amplified in the pluripotent stem cell; using a computer to compare the level of cDNA expression of the measured set of at least 12 early developmental genes to the level of expression of the same early developmental genes in a control pluripotent stem cell sample, and based on this comparison, determining the differentiation potential of the pluripotent stem cell line.

Metadata:
- Claim Count in Document: 30.0
- Percentile: 93.0
- Lexical Diversity: 2.01235
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14936774', '13114102', '12664763', '14439037', '13998974']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5764602104188021
- 35 USC 102 Novelty (BERT): 0.5338826545580436
- Combined Prediction Score: 0.5722024548327262
- Mean Citation Score: 351.49161200000015
- Max Citation Score: 374.56046
- Similarity Product: 229.52448882394555

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test