PATENT CLAIM ANALYSIS

Application Number: 16181261
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-02
Patent Classification: ["435", "325000"]

Abstract:
The present disclosure relates generally to novel methods and compositions for using engineered reprogramming factor(s) for the creation of induced pluripotent stem cells (iPSCs) through a kinetically controlled process. Specifically, this disclosure relates to establishing combinations of reprogramming factors, including fusions between conventional reprogramming factors with transactivation domains, optimized for reprogramming various types of cells. More specifically, the exemplary methods disclosed herein can be used for creating induced pluripotent stem cells from various mammalian cell types, including human fibroblasts. Exemplary methods of feeder-free derivation of human induced pluripotent stem cells using synthetic messenger RNA are also disclosed.

Claim (Index 40):
The method of  claim 32 , wherein in step b) of the method further comprises plating the cells on a feeder-free surface at a cell density of about 15,000 cells/ml; 25,000 cells/ml; 50,000 cells/ml; 75,000 cells/ml; 100,000 cells/ml; or 150,000 cells/ml in xeno-free culture medium and culturing the non-human primate cells under feeder-free, xeno-free and integration-free conditions; and;\n wherein step c) of the method further comprises transfecting the cells with varying doses of about 50 ng/ml to about 800 ng/ml of the size-exclusion chromatography purified synthetic mRNA reprogramming factors during each medium change, wherein lower doses of the synthetic mRNA reprogramming factors are used at earlier time points than later time points.

Metadata:
- Claim Count in Document: 83.0
- Percentile: 98.0
- Lexical Diversity: 1.58571
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['14292317', '13893166', '15148230', '12962498', '14322526']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6296173485983907
- 35 USC 102 Novelty (BERT): 0.6206580308121719
- Combined Prediction Score: 0.6287214168197689
- Mean Citation Score: 465.348888
- Max Citation Score: 633.7559
- Similarity Product: 485.98509381290086

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test