PATENT CLAIM ANALYSIS

Application Number: 16007424
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-10
Patent Classification: ["435", "194000"]

Abstract:
A DNA polymerase, having an amino acid sequence represented by SEQ ID NO:2, or a derivative of the amino acid sequence by substitution, deletion, or addition of at least one amino acid residue. The DNA polymerase is a hybrid DNA polymerase prepared by inserting a thioredoxin binding domain (TBD) of bacteriophage T7 DNA polymerase into a DNA polymerase I (Sau) of  Staphylococcus aureus . A method for preparing the DNA polymerase includes: 1) determining a corresponding position and a target substitution sequence in Sau protein for the TBD of the bacteriophage T7 DNA polymerase; 2) devising and synthesizing a primer according to a gene sequence of Sau and a sequence TBD published by GenBank; 3) cloning the Sau-TBD segment acquired in (2) to an expression vector pTrc99A to construct a recombinant vector pTrc99A-Sau-TBD; and 4) transforming  Escherichia coli  by the recombinant vector pTrc99A-Sau-TBD and inducing protein expression.

Claim (Index 5):
A method for preparing the polynucleotide sequence of SEQ ID NO:1, comprising:\n 1) determining a corresponding position and a target substitution sequence in Sau protein for the TBD of the bacteriophage T7 DNA polymerase; 2) devising and synthesizing a primer according to the gene sequence of Sau and the sequence TBD and amplifying by Overlap PCR to insert the TBD into the corresponding position of the gene sequence of Sau to prepare a Sau-TBD segment having a purification tag and a restriction site.

Metadata:
- Claim Count in Document: 9.0
- Percentile: 94.0
- Lexical Diversity: 1.9881
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15256732', '15119867', '13375264', '14135860', '14555863']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.4666420582587843
- 35 USC 102 Novelty (BERT): 0.6355185075670204
- Combined Prediction Score: 0.4835297031896079
- Mean Citation Score: 270.562496
- Max Citation Score: 591.5704
- Similarity Product: 551.4618297307014

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test