PATENT CLAIM ANALYSIS

Application Number: 15895907
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-11
Patent Classification: ["424", "185100"]

Abstract:
The present invention claims an isolated nucleotide sequence characterized by encoding the PFR1 protein of  Leishmania infantum  or a fragment thereof. This PFR1 protein or a fragment thereof comprises at least a selected immunodominant epitope between the following group: SEQ ID No: 1, SEQ ID No: 2, SEQ ID No: 3, SEQ ID No: 4, SEQ ID No: 5, SEQ ID No: 6, SEQ ID No: 7 and SEQ ID No: 8, where the immunodominant epitope is able to induce an antigen-specific T cell cytotoxic immune response in an animal, against the kinetoplastids causing the leishmaniasis disease. The immunodominant epitopes are cytotoxic T-lymphocyte activators and they present a high binding affinity for A2 type MHC Class I molecule.

Claim (Index 14):
The method of  claim 13 , comprising the steps of:\n (a) identifying at least one nucleotide sequence selected from the group consisting of:\n a nucleotide sequence coding for the PFR1 protein of  Leishmania infantum  of a fragment thereof comprising at least one immunodominant epitope selected from the epitope group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, and SEQ ID NO: 8, wherein the immunodominant epitope is able to induce an antigen-specific T cell cytotoxic immune response in the animal against the kinetoplastids causing the leishmaniasis disease; \n a nucleotide sequence coding for the 1-595 amino acids of  Leishmania infantum  PFR1 protein, determined by the SEQ ID NO: 12, and comprising at least one immunodominant epitope able to induce an antigen-specific T cell cytotoxic immune response against the kinetoplastids causing leishmaniasis disease in the animal; \n a nucleotide sequence coding for the 160-595 amino acids of  Leishmania infantum  PFR1 protein, determined by the SEQ ID NO: 9, and comprising at least one immunodominant epitope able to induce an antigen-specific T cell cytotoxic immune response against the kinetoplastids causing leishmaniasis disease in the animal; \n a nucleotide sequence coding for the 160-548 amino acids of  Leishmania infantum  PFR1 protein, determined by the SEQ ID NO: 10, and comprising at least one immunodominant epitope able to induce an antigen-specific T cell cytotoxic immune response against the kinetoplastids causing leishmaniasis disease in the animal; and \n a nucleotide sequence coding for the 160-385 amino acids of  Leishmania infantum  PFR1 protein, determined by the SEQ ID NO: 11, and comprising at least one immunodominant epitope able to induce an antigen-specific T cell cytotoxic immune response against the kinetoplastids causing leishmaniasis disease in the animal, \n (b) amplifying the at least one nucleotide sequence identified in (a) by PCR using genomic DNA from the kinetoplastid that causes the leishmaniasis disease, and oligonucleotides containing restriction enzymes sites that allow direct cloning of the amplicon into prokaryotic and/or eukaryotic expression vectors after its digestion with these restriction enzymes, (c) cloning that least one nucleotide sequence amplified in (b) for the production of the protein encoded by the at least one nucleotide sequence, (d) purifying the at least one protein(s) obtained in (c) by affinity chromatography, and (e) producing the vaccine for a safe inoculation.

Metadata:
- Claim Count in Document: 38.0
- Percentile: 88.0
- Lexical Diversity: 1.74684
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14234075', '13469636', '12149841', '14780504', '14288361']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7065984405236888
- 35 USC 102 Novelty (BERT): 0.6887985918258035
- Combined Prediction Score: 0.7048184556539002
- Mean Citation Score: 474.7210539999999
- Max Citation Score: 1011.5331
- Similarity Product: 677.4761979897737

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test