PATENT CLAIM ANALYSIS

Application Number: 15919640
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-07
Patent Classification: ["435", "006120"]

Abstract:
The present disclosure provides methods, arrays and kits for assessing the quality of genomic DNA samples, especially those obtained from formalin-fixed paraffin-embedded (FFPE) samples. The methods, arrays and kits provided herein use primer pairs specific to regions in the genomes of the organisms from which genomic DNA samples are obtained that have identical or nearly identical copies distributed across multiple chromosomes.

Claim (Index 1):
A method for assessing the quality of a test genomic DNA sample for downstream molecular DNA analyses, comprising:\n (a) performing one or more real-time PCR reactions that use genomic DNA in a test genomic DNA sample as templates in the presence of two or more primer pairs, wherein each of the two or more primer pairs is specific for amplifying identical or nearly identical genomic DNA fragments that are present at 10 or more different locations in the genome of the organism from which the test genomic DNA sample is obtained, wherein a genomic DNA fragment is nearly identical to another DNA if (i) the size difference between the two genomic DNA fragments is at most 5% of the full length of the longer fragment, and (ii) the sequence identity between the two fragments is at least 95%, (b) performing one or more real-time PCR reactions that use genomic DNA in a control genomic DNA sample as templates in the presence of the two or more primer pairs used in step (a), (c) determining the Ct values for the one or more real-time PCR reactions in step (a), (d) determining the Ct values for the one or more real-time PCR reactions in step (b), (e) determining the difference (\u0394Ct) between the Ct values determined in step (c) and the corresponding Ct values determined in step (d) for the one or more real-time PCR reactions, wherein the difference is indicative of the quality of the test genomic DNA sample, and (f) performing one or more additional molecular analyses of the genomic DNA test sample whose quality has been assessed as suitable for such analysis.

Metadata:
- Claim Count in Document: 41.0
- Percentile: 90.0
- Lexical Diversity: 1.36
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14208460', '14652098', '14775435', '14864158', '12678423']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.559428620920449
- 35 USC 102 Novelty (BERT): 0.6254198846603044
- Combined Prediction Score: 0.5660277472944345
- Mean Citation Score: 403.928404
- Max Citation Score: 607.0267
- Similarity Product: 515.2323613519907

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test