PATENT CLAIM ANALYSIS

Application Number: 15870659
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["435", "462000"]

Abstract:
Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.

Claim (Index 7):
A composition comprising:\n a nucleic acid encoding a variant  S. pyogenes  Cas9 protein comprising an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 18 with mutations at D10, E762, H983, D986, H840 or N863; and a nucleic acid encoding a hybrid guide nucleic acid that directs the variant  S. pyogenes  Cas9 protein to a target sequence; wherein the hybrid guide nucleic acid is selected from the group consisting of: (SEQ\u2003ID\u2003NO:\u20034) (X 17-20 )GUUUUAGAGCUAUGCUGUUUUG(X N );   (SEQ\u2003ID\u2003NO:\u20035) (X 17-20 )GUUUUAGAGCUA;   (SEQ\u2003ID\u2003NO:\u20036) (X 17-20 )GUUUUAGAGCUAUGCUGUUUUG;   (SEQ\u2003ID\u2003NO:\u20037) (X 17-20 )GUUUUAGAGCUAUGCU;   (SEQ\u2003ID\u2003NO:\u20038) (X 17-20 )GUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAG UCCG(X N );   (SEQ\u2003ID\u2003NO:\u20039) (X 17-20 )GUUUUAGAGCUAUGCUGAAAAGCAUAGCAAGUUAAAAU AAGGCUAGUCCGUUAUC(X N );   (SEQ\u2003ID\u2003NO:\u200310) (X 17-20 )GUUUUAGAGCUAUGCUGUUUUGGAAACAAAACAGCAUA GCAAGUUAAAAUAAGGCUAGUCCGUUAUC(X N );   (SEQ\u2003ID\u2003NO:\u200311) (X 17-20 )GUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAG UCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGC(X N );   (SEQ\u2003ID\u2003NO:\u200312) (X 17-20 )GUUUAAGAGCUAGAAAUAGCAAGUUUAAAUAAGGCUAG UCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGC;   (SEQ\u2003ID\u2003NO:\u200313) (X 17-20 )GUUUUAGAGCUAUGCUGGAAACAGCAUAGCAAGUUUAA AUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCG GUGC; and   (SEQ\u2003ID\u2003NO:\u200314) (X 17-20 )GUUUAAGAGCUAUGCUGGAAACAGCAUAGCAAGUUUAA AUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCG GUGC, wherein the X 17-20  is a sequence complementary to 17-20 consecutive nucleotides of the complementary strand of a target sequence, preferably a target sequence immediately 5\u2032 of a protospacer adjacent motif (PAM), wherein one or more of the nucleotides is a deoxyribonucleic acid, and N is 0-50.

Metadata:
- Claim Count in Document: 71.0
- Percentile: 86.0
- Lexical Diversity: 1.14286
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14775930', '14213723', '15415431', '15430218', '14213479']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7429749759459392
- 35 USC 102 Novelty (BERT): 0.628400063901057
- Combined Prediction Score: 0.731517484741451
- Mean Citation Score: 692.2233120000003
- Max Citation Score: 731.4343
- Similarity Product: 478.2494208582878

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test