PATENT CLAIM ANALYSIS

Application Number: 16067319
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-01
Patent Classification: ["435", "006110"]

Abstract:
The invention relates to a method and means for diagnosing tumors, in particular for an early diagnosis (prevention) and for differentiating between benign and malignant tumors using a PCR, in particular in bodily fluids. The method according to the invention is characterized by a combination of a pre-amplification process using a PCR, wherein methylated DNA sequences are amplified more strongly than non-methylated DNA sequences, and a subsequent quantification process using a special digital PCR, in which significantly more DNA is used than normally in the prior art. As comparison data indicates, the invention advantageously allows a clear reliable conclusion as to whether a malignant tumor disease is present or not. The invention is suitable for screening (prevention), monitoring the progress of a tumor disease, in particular to order to exclude a minimal residual disease (MRD), and for a differential diagnosis between malignant carcinoma and benign tumors.

Claim (Index 6):
Method according to  claim 5 , wherein primers used in step b) for amplifying methylated and unmethylated DNA sequences in the genes AOX-1, SERPINE-1, thrombomodulin and/or septin-9 are selected from sequences 14 to 19, 63 to 76, 99 to 103, and 126 to 144, at least one of the following primer pairs preferably being used: SEQ SEQ Target Forward (5\u2032->3\u2032) ID Reverse (5\u2032->3\u2032) ID AOX1 TGGGTTGGATTTTAGGTTTTAG 14 CTCACCTTACGAC CG TTC 15 (180 bp)   AOX1 GTTGGATTTTAGGTTTTAGTAAG 18 GCC CG ATCCATTATAATATC 19 (138 bp)   SERPINE1 AGAG CG TTGTTAAGAAGA 16 CTCCTACCTAAAATTCTCAAAA 17 (123 bp)   TM GGT CG ATT CG TATGTTAGA 70 AAC CG TAC CG AAACAAAA 71 (125 bp)   TM TTTGTGTTTTTTTGTTT CG GTAC 101 CACC CG ACTA CG ACTCTA CG 102 (160 bp)   TM GTTTGGGGTTTTGGTTTTTG 74 GCAATC CG T CG CAAATCTAA 75 (166 bp)

Metadata:
- Claim Count in Document: 5.0
- Percentile: 94.0
- Lexical Diversity: 1.94118
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['14079649', '12159081', '11884406', '13224024', '12219378']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6763939019561237
- 35 USC 102 Novelty (BERT): 0.5330711773576428
- Combined Prediction Score: 0.6620616294962756
- Mean Citation Score: 361.254908
- Max Citation Score: 377.0466
- Similarity Product: 248.95931399785283

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test