PATENT CLAIM ANALYSIS

Application Number: 15763007
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-10
Patent Classification: ["424", "423000"]

Abstract:
A composite material for bone repair based on a decellularized biological tissue matrix material and a preparation method thereof. The composite material for bone repair comprises an organic phase of a microfibrillar decellularized animal tissue matrix material and an inorganic phase of a calcium salt bioceramic or other inorganic bioglass. A preparation process for the composite material for bone repair does not need physical or chemical crosslinking. The composite material for bone repair has a three-dimensional porous network structure, and protein components in the biological tissue matrix material maintain a natural triplex structure. The composite material for bone repair has excellent biocompatibility, biodegradability, osteoconductivity, osteoinductivity, and osteogenecity, also has certain mechanical strength and shape memory function, and can be used as a bone filling material or a repair material for large-area bone defect.

Claim (Index 8):
A preparation method of the composite material for bone repair according to  claim 1 , characterized in that it comprises the following steps:\n 8.1) preparing a microfibrillar decellularized biological tissue matrix material, comprising the following steps:\n (a) collecting raw tissue material, rinsing off blood, cutting into desired size, and preserving the material at a low temperature of 2-10\u00b0 C.; \n (b) disinfecting and sterilizing: sterilizing the raw tissue material in a disinfectant solution, washing sufficiently with sterile deionized water, and then rinsing with a sterile physiological saline; \n (c) cutting: cutting the raw tissue material into a desired size, so as to facilitate tissue smashing and decellularization; \n (d) smashing the tissue: smashing and homogenizing the disinfected raw tissue material by a grinder; \n (e) soaking the material in a series of decellularization solutions to remove cells, and degrading the remaining desoxyribonucleic acid with deoxyribonuclease solution; \n (f) washing the tissue matrix: washing the product in step (e) by physiological saline with a mass concentration of 0.9%, and removing the supernatant obtained via the treatment of step (e) by centrifugation process; \n (g) terminally sterilizing: dispersing the product in step (f) into a solvent of a physiological saline or a phosphate buffer saline in a concentration of 10-40 mg/ml, sterilizing, and sealing the obtained decellularized biological tissue matrix microfibers in a closed container to preserve; \n 8.2) preparing bioceramic microparticles, comprising the following steps:\n (h) obtaining bioceramic microparticles with a particle size of 1-500 micrometers after steps of mechanical pulverization, high-rate ball-milling, and sieving of the bioceramics according to specific application, and then disinfecting and sterilizing the bioceramic microparticles under high temperature and high pressure to inactivate virus; \n (i) mixing the bioceramic microparticles with sterile physiological saline, sufficiently stirring and shocking to obtain a homogeneous suspension with a concentration of 500-1000 mg/ml; \n 8.3) preparing a composite material for bone repair, comprising the following steps:\n (j) physically mixing the microfibrillar decellularized biological tissue matrix material obtained in (g) of step 8.1) with the sterile particulate bioceramic obtained in (i) of step 8.2) homogeneously according to the ratio defined in  claim 1 , to obtain a mixture of composite material in a fluid state; \n (k) transferring the mixture of composite material in (j) of step 8.3) into a mold, placing the mold filled with the composite materials at a temperature of \u221220\u00b0 C. to \u2212196\u00b0 C. for 1-24 hours to obtain a frozen mixture; \n (l) removing the solvent from the mixture at a frozen state obtained in (k) of step 8.3) at a low temperature of \u221220\u00b0 C. to 10\u00b0 C. and under a vacuum environment of 10-2000 millitorr for 12-96 hours, obtaining a three-dimensional porous composite material for bone repair; \n (m) terminally sterilizing the composite material for bone repair obtained in ( 1 ) of step 8.3).

Metadata:
- Claim Count in Document: 13.0
- Percentile: 90.0
- Lexical Diversity: 1.9863
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['09284297', '10534794', '12188127', '09993739', '14728336']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7339386276913296
- 35 USC 102 Novelty (BERT): 0.5132157868614754
- Combined Prediction Score: 0.7118663436083441
- Mean Citation Score: 257.50511800000004
- Max Citation Score: 277.0647
- Similarity Product: 172.9751262109995

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test