PATENT CLAIM ANALYSIS

Application Number: 16067319
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2019-01
Patent Classification: ["435", "006110"]

Abstract:
The invention relates to a method and means for diagnosing tumors, in particular for an early diagnosis (prevention) and for differentiating between benign and malignant tumors using a PCR, in particular in bodily fluids. The method according to the invention is characterized by a combination of a pre-amplification process using a PCR, wherein methylated DNA sequences are amplified more strongly than non-methylated DNA sequences, and a subsequent quantification process using a special digital PCR, in which significantly more DNA is used than normally in the prior art. As comparison data indicates, the invention advantageously allows a clear reliable conclusion as to whether a malignant tumor disease is present or not. The invention is suitable for screening (prevention), monitoring the progress of a tumor disease, in particular to order to exclude a minimal residual disease (MRD), and for a differential diagnosis between malignant carcinoma and benign tumors.

Claim (Index 4):
Method according to  claim 3 , wherein the primers used in step b) for pre-amplifying methylated and unmethylated DNA sequences in the genes PLA2R1, RASSF1A and GSTP1 are selected from sequences 1 to 13, 53, 61, 62, 91 to 98, and 104 to 111, at least one of the following primer pairs preferably being used: SEQ SEQ Target Forward (5\u2032->3\u2032) ID Reverse (5\u2032->3\u2032) ID PLA2R1 GGGGTAAGGAAGGTGGAGAT 1 ACAAACCACCTAAATTCTAATAAACAC 2 (168 bp)   PLA2R1 GGAAGGTGGAGATTA CG G 7 G CG AATTTACAA CG AACAA C 8 (133 bp)   RASSF1A GTTTGTTAG CG TTTAAAGTTAG 3 AATA CG ACCCTTCCCAAC 4 (117 bp)   RASSF1A GTTTGGATTTTGGGGGAG C 106 ACTTTAAA CG CTAACAAA CG 107 \u2003(82 bp)   RASSF1A TTTGGATTTTGGGGGAG CG 108 CGCTAACTTTAAA CG CTAAC 109 \u2003(82 bp)   GSTP1 GTGAAG CG GGTGTGTAAGTTT 5 TAAACAAACAACAAAAAAAAAAC 6 (120 bp)   GSTP1 AT CG TAG CG GTTTTAGGGAA 93 TCCCCAA CG AAACCTAAAAA 94 (116 bp)

Metadata:
- Claim Count in Document: 5.0
- Percentile: 94.0
- Lexical Diversity: 1.94118
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['14079649', '12159081', '11884406', '13224024', '12219378']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6757380333765729
- 35 USC 102 Novelty (BERT): 0.5333385549991513
- Combined Prediction Score: 0.6614980855388307
- Mean Citation Score: 361.254908
- Max Citation Score: 377.0466
- Similarity Product: 274.3436377266884

Labels:
- Claim Label 101: 0
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 0

Dataset: test