PATENT CLAIM ANALYSIS

Application Number: 15865574
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["424", "093210"]

Abstract:
The technology described herein relates to methods and kits for directed differentation of primordial NKX2-1+ lung progenitors along proximal differentiation pathways into functional airway epithelial cells and airway organoids (“bronchospheres”) or along distal lineage pathways using modulation of Wnt signaling. Other aspects relate cell lines, methods, assays and kits comprising airway epithelial cells, and assays for diagnosing a disease that affects swelling of the bronchospheres, and/or for assessing genetic lesions and/or drugs for treating the the disease, where the disease is cystic fibrosis. Other aspects relate to personalized medicine and methods of treatment of cystic fibrosis using the airway epithelial cells.

Claim (Index 17):
The method of  claim 16 , wherein the wnt inhibitory agent is withdrawal of a Wnt activator, or is an agent which inhibits Wnt or Wnt3 selected from any of:\n a. Wls/Evi, Frizzled, Dsh (disheveled), LRP-5, LRP-6, Dally, Dally-like, PAR1, \u03b2-catenin, TCF, lef-1 or Frodo; b. a RNAi agent which inhibits the RNA transcript of Wls/Evi; c. SEQ ID NO:1 (CACAAATCCTTTCTACAGTAT)) (siWLS-A) or SEQ ID NO:2 (GGGTTACCGTGATGATATG) (siWLS-B); d. Dickkopf-1 (DKK1), WIF-1, cerberus, secreted frizzled-related proteins (sFRP), sFRP-1, sFRP-2, collagen 18 (collagen XVIII), endostatin, carboxypeptidase Z, receptor tyrosine kinase, corin, Dg1, Dapper, pertussis toxin, naked, Frz-related proteins or LRP lacking the intracellular domain; e. an inhibitor of \u03b2-catenin is selected from the group consisting of; protein phosphatase 2A (PP2A), chibby, promtin 52, Nemo/LNK kinase, MHG homobox factors, XSox17, HBP1, APC, Axin, disabled-2 (dab-2) and gruncho (grg); f. increases the activity and/or expression of GSK-3 and/or GSK3\u03b2 or is a peptide of GSK3\u03b2; g. an agent which activates the PKB pathway or wortannin; or h. a peptide of DKK1.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 86.0
- Lexical Diversity: 1.66176
- Patent Class: 424.0
- Transitional Phrase Type: closed
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15635975', '14371627', '14367061', '15223603', '14417273']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.8132906196244853
- 35 USC 102 Novelty (BERT): 0.532213062355505
- Combined Prediction Score: 0.7851828638975874
- Mean Citation Score: 358.088658
- Max Citation Score: 371.3459
- Similarity Product: 233.60351111575363

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test