PATENT CLAIM ANALYSIS

Application Number: 15575988
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2018-08
Patent Classification: ["800", "018000"]

Abstract:
An object of the present invention is to provide a mouse that enables the functions of human NK cell to be studied. A DNA consisting of a nucleotide sequence represented by SEQ ID NO: 1, which is a gene region comprising a DNA in which a cDNA sequence encoding interleukin 15 (IL-15) is operably ligated to a cDNA sequence encoding the signal peptide of human interleukin (IL-2), is inserted to immunodeficient mouse cDNA. In NOD-scid, IL-2rγ null -hIL-15 Tg mice thus generated, hCD56 +  cell having a concentration sufficient for conducting in vivo study on human mature NK cell are detected for at least 6 months after transplantation.

Claim (Index 8):
A method for generating a NOD-scid, IL-2r\u03b3 null -hIL-15 Tg mouse, comprising the following steps (1) to (7) sequentially:\n (1) introducing a DNA comprising a nucleotide sequence represented by SEQ ID NO: 1 to a vector comprising a region necessary for inserting a DNA consisting of the nucleotide sequence represented by SEQ ID NO: 1 to a mouse genomic DNA, thereby generating a vector for DNA preparation for injection into a mouse fertilized egg having the DNA comprising the nucleotide sequence represented by SEQ ID NO: 1; (1-1) optionally preparing a DNA fragment for injection into fertilized eggs wherein the DNA fragment comprises the DNA consisting of the nucleotide sequence represented by SEQ ID NO: 1, and a region necessary for insertion of the DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 to mouse genomic DNA; (2) injecting the vector generated in the step (1) and/or the vector fragment prepared in the step (1-1) into a fertilized egg of an interleukin 2 receptor \u03b3 chain gene (IL-2R\u03b3)-knockout mouse, thereby generating an injected-fertilized egg; (3) culturing the injected-fertilized egg generated in the step (2), thereby generating a newborn mouse; (4) determining whether or not the DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 is inserted in genomic DNA of a NOD-IL-2r\u03b3 null  mouse among the mouse generated in the step (3); (5) determining whether or not the mouse determined in the step (4) that the DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 is inserted in genomic DNA of the NOD-IL-2r\u03b3 null  mouse secretes hIL-15, thereby selecting a hIL-15-secreting mouse as a NOD-IL-2r\u03b3 null -hIL-15 Tg mouse; (6) mating the NOD-IL-2r\u03b3 null -hIL-15 Tg mouse with a NOG mouse, thereby generating a NOD-scid, IL-2r\u03b3 null -hIL-15 Tg mouse containing scid mutation; and (7) mating the NOD-scid, IL-2r\u03b3 null -hIL-15 Tg mouse with a NOG mouse, thereby generating a NOD-scid, IL-2r\u03b3 null -hIL-15 Tg mouse and a NOG mouse at a ratio of 1:1.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 91.0
- Lexical Diversity: 1.5
- Patent Class: 800.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['10221549', '12620469', '11914649', '14420318', '10533796']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.604868212848233
- 35 USC 102 Novelty (BERT): 0.5528857374362254
- Combined Prediction Score: 0.5996699653070322
- Mean Citation Score: 401.718952
- Max Citation Score: 469.75204
- Similarity Product: 352.2207359876157

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test