PATENT CLAIM ANALYSIS

Application Number: 15907470
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-09
Patent Classification: ["435", "440000"]

Abstract:
The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GOI genome target location and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts from that point on.

Claim (Index 11):
Method according to  claim 10 , wherein exchange of template DNA construct to said modified template DNA constructs is achieved in the following way;\n (a) each template DNA construct is designed to have conserved sequence stretches in their 5\u2032- and 3\u2032-ends that are homologous to said genomic region; (b) each template DNA construct is designed to have a gene editing nuclease target sequence were the sequence differs between generation z and z+1; (c) template DNA constructs of generation z and z+1 contains different selection marker(s); (d) a template DNA construct of generation z+1 is introduced together with a gene editing expression vector construct into a cell or cell population containing a DNA construct of generation z and wherein the gene editing expression vector codes for a gene editing nuclease with specificity for said target sequence of the template DNA construct of generation z; (e) cells having undergone the correct exchange via double strand break catalyzed cellular repair mechanisms are enriched for by using the difference in selection markers between DNA constructs of generation z and z+1; and (f) optionally, DNA analysis methods are applied to ensure correct exchange only for cells passed on to next step.

Metadata:
- Claim Count in Document: 24.0
- Percentile: 88.0
- Lexical Diversity: 1.38462
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['14967689', '14092786', '13752647', '12793898', '12507252']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6134016617752855
- 35 USC 102 Novelty (BERT): 0.5248559758171464
- Combined Prediction Score: 0.6045470931794716
- Mean Citation Score: 308.898376
- Max Citation Score: 313.97794
- Similarity Product: 213.0539996599865

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test