PATENT CLAIM ANALYSIS

Application Number: 16179322
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-02
Patent Classification: ["424", "204100"]

Abstract:
A method for producing AAV, without requiring cell lysis, is described. The method involves harvesting AAV from the supernatant. For AAV having capsids with a heparin binding site, the method involves modifying the AAV capsids and/or the culture conditions to ablate the binding between the AAV heparin binding site and the cells, thereby allowing the AAV to pass into the supernatant, i.e., media. Thus, the method of the invention provides supernatant containing high yields of AAV which have a higher degree of purity from cell membranes and intracellular materials, as compared to AAV produced using methods using a cell lysis step.

Claim (Index 26):
A kit for production of an AAV particle lacking a heparin binding site from the supernatant, said kit comprising one or more:\n (a) a production cell capable of directing the packaging of an AAV viral particle, whereby the AAV particle is secreted into the media without disruption of the production cell, wherein the AAV particles comprise an AAV capsid and a nucleic acid molecule which comprises an AAV 5\u2032 inverted terminal repeat (ITR), a nucleic acid sequence encoding a gene product, and a 3\u2032 ITR, wherein the cell comprises (i) a coding sequence for the AAV capsid under control of sequences which direct its expression in the packaging cell, (ii) an AAV rep coding sequence which expresses a rep protein in the cell to permit packaging of the nucleic acid molecule into the AAV capsid, and (iii) one or more helper functions required for packaging the nucleic acid molecule into the AAV capsid, wherein the AAV capsid lacks a heparin binding site, and wherein the kit further comprises one or more of: (b) a transfection reagent; (c) a plasmid component for construction of a vector; (d) a component necessary for collection, purification, concentration or harvest of the assembled AAV particle; (e) a reagent for negative or positive selection of viral particle in order to purify; (f) a reagent for concentration of viral preparation; and (g) a reagent for enzymatic digestion of contaminants in viral preparation.

Metadata:
- Claim Count in Document: 68.0
- Percentile: 98.0
- Lexical Diversity: 1.79365
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14919801', '12226588', '13884914', '10511980', '10798192']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7014846910176675
- 35 USC 102 Novelty (BERT): 0.5588399661570735
- Combined Prediction Score: 0.6872202185316081
- Mean Citation Score: 317.796398
- Max Citation Score: 426.3658
- Similarity Product: 339.509823408246

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test