PATENT CLAIM ANALYSIS

Application Number: 15873699
Application Type: Utility
Filing Date: 2018-01
Publication Date: 2018-07
Patent Classification: ["800", "278000"]

Abstract:
Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR associated protein 9 (Cas9) offer an effective way of creating targeted mutagenesis in plants. To alleviate concerns related to genetically modified plants, the present disclosure provides a novel and efficient genome editing system that allows the regeneration of mutant plants without DNA stable integration. This DNA free system utilizes Cas9 mRNA, guide RNA and selectable marker RNA to infect plant protoplasts. After a short period of selection of the transfected cells, non-transgenic plants carrying expected mutations were regenerated. The system offers a way of creating desired mutants without transgenic elements.

Claim (Index 1):
A method for selecting cells carrying a transfected nucleic acid comprising the steps of:\n exposing a plurality of cells to a first RNA molecule under conditions sufficient to promote transfection of the first RNA molecule into one or more of the plurality of cells, wherein the first RNA molecule or a peptide or protein encoded by the first RNA molecule permits selection of the one or more of the plurality of cells transfected with the first RNA molecule based on a first selectable property; and selecting the one or more cells of the plurality of cells transfected with the first RNA molecule based on the first selectable property.

Metadata:
- Claim Count in Document: 15.0
- Percentile: 86.0
- Lexical Diversity: 1.41772
- Patent Class: 800.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14885140', '14523799', '14183486', '14258458', '14577997']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5538125364422735
- 35 USC 102 Novelty (BERT): 0.4963755282052474
- Combined Prediction Score: 0.5480688356185709
- Mean Citation Score: 263.98526400000003
- Max Citation Score: 285.56033
- Similarity Product: 220.56634548719825

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test