PATENT CLAIM ANALYSIS

Application Number: 16261311
Application Type: Utility
Filing Date: 2019-01
Publication Date: 2019-05
Patent Classification: ["435", "069100"]

Abstract:
Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

Claim (Index 38):
A method of regulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture process, comprising transfecting a mammalian host cell to overexpress a protein that is involved in an N-glycosylation pathway, wherein the protein involved in the N-glycosylation pathway is selected from the group consisting of N-acetyl-glucosaminyltransferase-1 (encoded by Mgat1); N-acetyl-glucosaminyltransferase-2 (encoded by Mgat2); a UDP-Galactose transporter encoded by Slc35a2; and combinations thereof; and either\n (a) the mammalian host cells are subjected to a temperature shift; or (b) the mammalian host cells are induced into cell growth arrest by L-asparagine starvation followed by perfusion with a serum-free perfusion media having an L-asparagine concentration of 5 mM or less.

Metadata:
- Claim Count in Document: 62.0
- Percentile: 99.0
- Lexical Diversity: 1.30769
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['16130879', '15115615', '16039123', '14289943', '14776404']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6173978431524029
- 35 USC 102 Novelty (BERT): 0.711507504583638
- Combined Prediction Score: 0.6268088092955264
- Mean Citation Score: 508.46683
- Max Citation Score: 785.7541
- Similarity Product: 740.8007579609392

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test