PATENT CLAIM ANALYSIS

Application Number: 15775007
Application Type: Utility
Filing Date: 2018-05
Publication Date: 2018-12
Patent Classification: ["549", "264000"]

Abstract:
The invention relates to a novel method to isolate a natural product, a flavonoid namely inoscavin A from a Sri Lankan mushroom, medicinal preparation thereof to treat rhabdomyosarcoma cancer condition. This compound was isolated from the terrestrial basidiomycete  Fulviformes fastuosus  for the first time. The isolation method of the inoscavin A comprises the following steps of: (1) carrying out sonication extraction of pulverized specimen by methanol; (2) partitioning in to hexane, dichloromethane and ethyl acetate respectively by liquid-liquid extraction 3) fractionation using normal phase silica gel column chromatography 4) purification using preparative high performance liquid chromatography (HPLC) to obtain flavonoid compound inoscavin A in substantially purified form (≥98%). In vitro cytotoxicity experiments demonstrated that the composition comprising inoscavin A strongly induced the apoptosis of human rhabdomyosarcoma (RD) cells compared to the cytotoxicity produced on normal epithelial cells (CC-1). Therefore, isolated flavonoid compound can be used for manufacturing of a medicament to treat rhabdomyosarcoma cancer condition. A method of treating rhabdomyosarcoma using medicinal preparation comprising inoscavin A can be achieved by administering said composition to a subject in need of such treatment.

Claim (Index 5):
The purification using high performance liquid chromatography as claimed in  claim 1  comprises the steps of, carrying out recycling preparative high performance liquid chromatography (HPLC) for dried fraction eluting with a linear gradient from 1:9 to 3:7 water: methanol acidified with 0.04% trifluoroacetic acid at a flow rate of 6 mL/min, using C 18  preparative column. Eluent was dried under reduced pressure to give a pale yellow amorphous powder, namely inoscavin A refined product.

Metadata:
- Claim Count in Document: 17.0
- Percentile: 93.0
- Lexical Diversity: 1.70492
- Patent Class: 549.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['11257128', '13329601', '13364098', '13364127', '11395734']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6868917608747263
- 35 USC 102 Novelty (BERT): 0.5087464252960806
- Combined Prediction Score: 0.6690772273168617
- Mean Citation Score: 194.947806
- Max Citation Score: 218.79735
- Similarity Product: 142.95849864676595

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test