PATENT CLAIM ANALYSIS

Application Number: 16227899
Application Type: Utility
Filing Date: 2018-12
Publication Date: 2019-06
Patent Classification: ["435", "007100"]

Abstract:
The present invention relates to a method for detecting molecules. The method employs: at least two primary antibodies, wherein the first primary antibody binds to a first site on a molecule and the second primary antibody binds to a second site on a molecule, wherein the second site is different from the first site and wherein the first and second primary antibodies are immunologically distinct; at least two secondary antibodies, wherein the first secondary antibody is labelled with a fluorescence resonance energy transfer (FRET) donor and binds to the first primary antibody; and the second secondary antibody is conjugated or fused to an enzyme and binds the second primary antibody, wherein the first secondary antibody does not bind the second primary antibody and the second secondary antibody does not bind the first primary antibody; a conjugate comprising a FRET acceptor and a substrate specific for the enzyme, wherein when the substrate reacts with the enzyme, an activated conjugate forms, which activated conjugate binds to electron rich moieties on a molecular surface adjacent to the enzyme; wherein the method comprises: contacting a sample with the at least two primary antibodies; contacting the sample with the at least two secondary antibodies; performing a wash step; contacting the sample with the conjugate; and detecting any FRET signal generated by the FRET acceptor.

Claim (Index 56):
The in vitro method of  claim 53  wherein the substrate is tyramine; and/or wherein the primary antibodies are unlabelled.

Metadata:
- Claim Count in Document: 146.0
- Percentile: 98.0
- Lexical Diversity: 2.96296
- Patent Class: 435.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['14778532', '15121962', '14118747', '12516333', '12854144']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6342975641370642
- 35 USC 102 Novelty (BERT): 0.6522155105595082
- Combined Prediction Score: 0.6360893587793086
- Mean Citation Score: 379.081274
- Max Citation Score: 863.5623
- Similarity Product: 582.672628501922

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test