PATENT CLAIM ANALYSIS

Application Number: 16084998
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-03
Patent Classification: ["530", "356000"]

Abstract:
The present disclosure provides methods for capturing recombinant collagen 7 from cell culture and methods of purifying recombinant collagen 7. Also provided are compositions comprising purified recombinant collagen 7 that may be used for administration to humans.

Claim (Index 9):
A method for purifying recombinant human collagen 7, the method comprising:\n a) subjecting a sample containing recombinant human collagen 7 to a mixed mode, flow through resin, wherein the mixed mode flow through resin comprises a ligand activated core and an inactive shell, wherein the inactive shell prevents recombinant human collagen 7 from entering the ligand activated core and wherein impurities that are small enough pass through the inactive shell and bind to the ligand activated core; b) collecting a first effluent that flows through the mixed mode, flow through resin and contains recombinant human collagen 7; c) subjecting the first effluent from step b) to a cation exchange chromatography resin under conditions permitting the recombinant human collagen 7 to bind to the cation exchange chromatography resin; d) eluting recombinant human collagen 7 from the cation exchange chromatography resin; e) subjecting the eluted recombinant human collagen 7 from step d) to a hydrophobic interaction chromatography resin in flow through mode and collecting a second effluent that flows through the hydrophobic interaction chromatography resin; f) subjecting the second effluent of step f) to an ultrafiltration step to concentrate the recombinant human collagen 7; g) dialfiltering the concentrated recombinant human collagen 7 from step f) with a buffer; and h) recovering purified recombinant human collagen 7, wherein the method comprises one or more viral reduction steps and an endonuclease treatment step.

Metadata:
- Claim Count in Document: 20.0
- Percentile: 97.0
- Lexical Diversity: 1.3
- Patent Class: 530.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['10532998', '11255800', '13262622', '11575833', '16065512']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6636422621170341
- 35 USC 102 Novelty (BERT): 0.4941094365870361
- Combined Prediction Score: 0.6466889795640344
- Mean Citation Score: 220.145652
- Max Citation Score: 230.56027000000003
- Similarity Product: 161.32596132234576

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test