PATENT CLAIM ANALYSIS

Application Number: 16229657
Application Type: Utility
Filing Date: 2018-12
Publication Date: 2019-05
Patent Classification: ["435", "006120"]

Abstract:
Methods of detecting or quantifying short RNA or DNA molecules using split cycle amplification are provided.

Claim (Index 1):
A method of generating and amplifying cDNA, the method comprising:\n (a) reverse transcribing a target RNA comprising a non-polyA region and a polyA tail by:\n (i) hybridizing a reverse transcription (RT) primer to the target RNA, wherein the RT primer comprises from 5\u2032 to 3\u2032:\n X-(T) m -Y-(T) n -Z \n \n wherein X is an optional 5\u2032 tail nucleotide sequence of 1-10 nucleotides that is not complementary to the target RNA; T is thymine; Y is a single nucleotide that is C, G, or A; Z is an optional sequence of 1-10 nucleotides that is complementary to a portion of the non-polyA region adjacent to the polyA tail; m is 1-20; and n is 1-20; and\n (ii) reverse transcribing the target RNA by extending the RT primer with a RNA-dependent polymerase to generate a cDNA incorporating the RT primer; and \n (b) amplifying the cDNA by:\n (i) hybridizing an amplification primer comprising a sequence complementary to the -(T) m -Y-(T) n , and \n (ii) extending the amplification primer with a DNA-dependent polymerase.

Metadata:
- Claim Count in Document: 41.0
- Percentile: 98.0
- Lexical Diversity: 1.0625
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14600550', '15287254', '13264122', '10077383', '10934890']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6736333540796617
- 35 USC 102 Novelty (BERT): 0.520921678274729
- Combined Prediction Score: 0.6583621864991686
- Mean Citation Score: 339.27292
- Max Citation Score: 351.52637000000016
- Similarity Product: 281.37382372408763

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test