PATENT CLAIM ANALYSIS

Application Number: 15925656
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-09
Patent Classification: ["382", "128000"]

Abstract:
Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. These have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy of polynucleotide sequencing applications.

Claim (Index 13):
A method for accurately determining a relative position of analytes immobilized on the surface of a densely packed substrate, comprising:\n a. providing a substrate comprising a surface, wherein the surface comprises a plurality of analytes immobilized on the surface at discrete locations; b. performing a plurality of cycles of probe binding and signal detection on said surface, each cycle comprising:\n i. contacting said analytes with a plurality of probes from a probe set, wherein said probes comprise a detectable label, wherein each of said probes binds specifically to a target analyte; and \n ii. imaging a field of said surface with an optical system to detect a plurality of optical signals from individual probes bound to said analytes at discrete locations on said surface; \n c. determining a peak location from each of said plurality of optical signals from images of said field from at least two of said plurality of cycles; and d. overlaying said peak locations for each optical signal and applying an optical distribution model at each cluster of optical signals to determine a relative position of each detected analyte on said surface with improved accuracy.

Metadata:
- Claim Count in Document: 26.0
- Percentile: 90.0
- Lexical Diversity: 1.2973
- Patent Class: 382.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14366486', '12009659', '13835492', '14922038', '15207892']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.2686575008507681
- 35 USC 102 Novelty (BERT): 0.4938753548733257
- Combined Prediction Score: 0.2911792862530239
- Mean Citation Score: 183.510428
- Max Citation Score: 219.60469
- Similarity Product: 163.1279654342747

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test