PATENT CLAIM ANALYSIS

Application Number: 16079440
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2019-05
Patent Classification: ["436", "514000"]

Abstract:
Provided is a single-channel chemiluminescent micro-fluidic chip, including a chip body with a quantification-reaction cavity and a waste liquid cavity. The quantification-reaction cavity is composed of a quantification-reaction pool on a lower portion and a reaction pool cover plate, the quantification-reaction pool is equally divided into three cavities by two partition plates, a labeled antibody is placed in the middle cavity, coated antibodies are placed in the others, a surface, facing the quantification-reaction pool, of the reaction pool cover plate is equally divided into two parts. In result, the coated antibodies and the labeled antibodies are physically separated to effectively avoid nonspecific binding. Due to a special structure of the reaction tank, wavy fluid flow is generated to fully mix the coated antibodies and the labeled antibodies, which improves testing efficiency and sensitivity.

Claim (Index 10):
A detection method of a single-channel chemiluminescent micro-fluidic chip, characterized by including the following steps:\n Step 1, add a whole blood sample into a whole blood filter cavity via a sample introduction port; Step 2, after a chip body is incubated for a period of time t 1 , make the chip body in contact with an external air path, external fluid paths and a pressing mechanism, so that a cleaning solution delivery branch and a chromogenic reagent delivery branch of the chip body are communicated with the corresponding external fluid paths and the whole blood filter cavity is communicated with the external air path, and that in a valve device, a pressing head of a pressing mechanism makes contact with an inner sealing film of a conductive sealing ring and a capacitance probe makes contact with a capacitance probe contact of the conductive sealing ring; Step 3, slowly inflate the chip body by the external air path, so that the whole blood sample is promoted to turn into plasma after being filtered by whole blood filter paper and then enters the whole blood filter cavity; Step 4, under the continuous air pressure from the external air path, the plasma in the whole blood filter cavity continues to flow forward through a first anti-backflow device and a quantification-reaction cavity in sequence along a micro-fluidic passage and then to flow into the valve device, at this moment, trigger the valve device to close the micro-fluidic passage once the plasma touches the conductive sealing ring of the valve device and then the capacitance probe senses capacitance changes of the capacitance probe contact, in contact with the capacitance probe, of the conductive sealing ring, and at the same time, close the external air path to stop pressurization; Step 5, separate the chip body from the external air path, the external fluid paths and the pressing mechanism, and evenly mix the plasma in the quantification-reaction cavity with coated antibodies and a labeled antibody is embedded in the quantification-reaction cavity for 3-10 minutes for an immune reaction; Step 6, make the chip body in contact with the external air path, the external fluid paths and the pressing mechanism, so that the cleaning solution delivery branch and the chromogenic reagent delivery branch of the chip body are communicated with the corresponding external fluid paths and the whole blood filter cavity is communicated with the external air path, and that in the valve device, the pressing head of the pressing mechanism makes contact with the inner sealing film of the conductive sealing ring and the capacitance probe makes contact with the capacitance probe contact of the conductive sealing ring; Step 7, start the external air path, so that the plasma in the chip body is pushed by an air pressure from the external air path to flow along the micro-fluidic passage till the plasma in the sample introduction port and the micro-fluidic passage is dried, and at this moment, close the external air path when a capacitance value sensed by the capacitance probe returns to an initial value; Step 8, start a cleaning solution device in the corresponding external fluid path to make a cleaning solution sequentially flow through the second anti-backflow device disposed on the cleaning solution delivery branch and the quantification-reaction cavity to reach the valve device, trig the valve device to close the micro-fluidic passage once the cleaning solution touches the conductive sealing ring of the valve device and then the capacitance probe senses capacitance changes of the capacitance probe contact, in contact with the capacitance probe, of the conductive sealing ring, and at the same time, close the external air path to stop pressurization; Step 9, evenly mix the cleaning solution in the chip body and particularly in the quantification-reaction cavity for 1-3 minutes for cleaning; Step 10, start the external air path to provide an air pressure to push the cleaning solution to flow forwards to dry the chip body, and close the external air path when a capacitance value sensed by the capacitance probe returns to the initial value; Step 11, repeat steps 8-10 for 3-8 times; Step 12, start a chromogenic reagent device in the corresponding external fluid path to make a chromogenic reagent sequentially flow through the anti-backflow device disposed on the chromogenic reagent delivery branch and the quantification-reaction cavity to reach the valve device, trig the valve device to close the micro-fluidic passage once the chromogenic reagent touches the conductive sealing ring of the valve device and then the capacitance probe senses capacitance changes of the capacitance probe contact, in contact with the capacitance probe, of the conductive sealing ring, and at the same time, close the external air path to stop pressurization; Step 13, evenly mix the chromogenic reagent in the quantification-reaction cavity for 3-8 minutes for a color development reaction; and Step 14, separate the chip body from the external air path, the external fluid paths and the pressing mechanism to complete detection.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 96.0
- Lexical Diversity: 2.0137
- Patent Class: 436.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: True
- Related Applications: ['12876883', '12202414', '10801180', '13346615', '13004933']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6422748508927867
- 35 USC 102 Novelty (BERT): 0.4894494544013806
- Combined Prediction Score: 0.6269923112436461
- Mean Citation Score: 178.765272
- Max Citation Score: 189.7442
- Similarity Product: 123.56421585047244

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test