PATENT CLAIM ANALYSIS

Application Number: 16012002
Application Type: Utility
Filing Date: 2018-06
Publication Date: 2018-12
Patent Classification: ["435", "173600"]

Abstract:
A new simple transfection method using an integrated electrowetting nano-injector (“INENI”) with controlled dosage delivery and high transfection efficiency is disclosed. The volume of delivery can be controlled via voltage application to an inner and outer electrode integrated into a nano-pipette. With higher voltages, more liquid enters the INENI and with lower voltages liquid is expelled. This method can be used to deliver plasmid DNA directly into the nuclei of cells. The INENI requires only the use of a single probe since both electrodes are integrated into the same nano-pipette. Hence, more space is available, and ergo the INENI offers a simplistic means for direct injection of metered amounts of exogenous material into the confines of a cell cytoplasm and/or nucleus while retaining full cell viability.

Claim (Index 1):
A system for using an integrated electrowetting to stimulate an injection or an aspiration of a biomaterial, wherein the biomaterial is an aqueous solution ( 104 ) comprising an organic phase and an aqueous phase, the system comprising:\n (a) a nano-pipette ( 102 ) having an aperture ( 112 ) with dimensions in the nanoscale region, wherein an organic phase ( 105 ) is disposed in the nano-pipette ( 102 ); (b) an outer electrode ( 106 ) coupled to an outer surface of the nano-pipette ( 102 ); (c) an inner electrode ( 108 ) disposed in the organic phase ( 105 ) within the nano-pipette ( 102 ), wherein a potential difference is applied between the outer electrode ( 106 ) and the inner electrode ( 108 ) to electrically change a wetting angle at an interface ( 107 ) between the organic phase ( 105 ) and the aqueous solution ( 104 ), said change effectively stimulating the movement of the aqueous solution ( 104 ), wherein when the potential difference exceeds a threshold voltage, the aqueous solution ( 104 ) is extracted and drawn into the nano-pipette ( 102 ), and wherein when the potential difference is lower than the threshold voltage, the aqueous solution ( 104 ) is injected and drawn out of the nano-pipette ( 102 ).

Metadata:
- Claim Count in Document: 20.0
- Percentile: 94.0
- Lexical Diversity: 1.52747
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14282630', '15809702', '10560301', '13406269', '14775168']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5658351349677632
- 35 USC 102 Novelty (BERT): 0.4693297063045045
- Combined Prediction Score: 0.5561845921014373
- Mean Citation Score: 152.523126
- Max Citation Score: 156.78734
- Similarity Product: 114.27944423095704

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test