PATENT CLAIM ANALYSIS

Application Number: 16206168
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-05
Patent Classification: ["506", "016000"]

Abstract:
Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

Claim (Index 32):
A method of generating a barcoded nucleic acid fragment, comprising:\n (a) contacting a plurality of biological particles comprising chromatin with a plurality of transposase-nucleic acid complexes to generate a plurality of nucleic acid fragments of said chromatin; (b) partitioning said plurality of biological particles comprising said plurality of nucleic acid fragments and a plurality of barcoded beads into a plurality of partitions,\n wherein a partition of said plurality of partitions comprises a biological particle of said plurality of biological particles and a barcoded bead of said plurality of barcoded beads, \n wherein said biological particle comprises a nucleic acid fragment of said plurality of nucleic acid fragments, \n wherein said barcoded bead is attached to a plurality of nucleic acid barcode molecules comprising a common barcode sequence, and \n (c) generating a barcoded nucleic acid fragment using said nucleic acid fragment and a nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules.

Metadata:
- Claim Count in Document: 89.0
- Percentile: 98.0
- Lexical Diversity: 1.53521
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15842687', '15887947', '15933299', '15875899', '16052486']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6196800861274867
- 35 USC 102 Novelty (BERT): 0.6375320123047201
- Combined Prediction Score: 0.6214652787452101
- Mean Citation Score: 509.510024
- Max Citation Score: 724.95953
- Similarity Product: 576.7933126576758

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test