PATENT CLAIM ANALYSIS

Application Number: 15923717
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-09
Patent Classification: ["800", "267000"]

Abstract:
Ocimum basilicum  plants or germplasm having tolerance or improved tolerance to  Peronospora  belbahrii infection, comprising in its genome the single nucleotide polymorphisms (SNPs) of SEQ ID NOs:1-630 or having approximately 90% to 0.5% of said SNPs in said  Ocimum basilicum  genome are disclosed. Further embodiments include methods of producing, selecting, and evaluating the responses of  Ocimum basilicum  plants or germplasm having tolerance or improved tolerance to  Peronospora belbahrii  infection; said plants comprised of at least one dominant allele and at least one recessive allele in the homozygous state that produce higher levels of tolerance to  Peronospora belbahrii ; seeds, plants, plant parts, methods for producing basil plants by crossing said basil plant with itself or with another basil plant, methods for producing sweet basil plants having higher tolerance to  Peronospora belbahrii , plants and plant parts produced by those methods and methods for producing interspecific basil plants having tolerance to  Peronospora belbahrii.

Claim (Index 31):
A method of evaluating the response of an  O. basilicum  plant to  Peronospora belbahrii , comprising the steps of:\n sowing  O. basilicum  seeds into one or more plug trays and placed in conditions to promote germination, wherein said seeds are comprised of one or more lines for testing, a susceptible control, and a resistant control; growing said plants in said plug trays to the stage where each plant has developed two true leaves, and inoculating said plants with  Peronospora belbahrii  inoculum solution, wherein the inoculum solution consists of fresh harvested leaves where sporulation has recently taken place that are placed in a container with water and agitated, and wherein the inoculum solution is adjusted to a concentration of 50,000 spores per milliliter of water using a hemocytometer, and wherein the inoculum solution is applied to the upper surface of the leaves using a hand sprayer; transferring the inoculated plants in the plug trays to a closed plastic chamber for 16 hours, wherein the conditions in the chamber are total darkness, a temperature range of 19 degrees Celsius to 21 degrees Celsius, a relative humidity range between 85% to 90% for the first 4 hours, and for hours 4 through 16, the relative humidity is greater than 90%; recording the relative humidity inside the plastic chamber; removing the plants in the plug trays from the plastic chamber and transferring said plants to a different location with full sunlight under natural day length and night length for 6 days; transferring the plants in the plug trays back into a plastic chamber for about 16 hours, wherein the conditions in the chamber are total darkness, a temperature range of 19 degrees Celsius to 21 degrees Celsius, a relative humidity range between 85% to 90% for the first 4 hours, and for hours 4 through 16, the relative humidity is greater than 90%; removing said plants and plug trays from the plastic chamber; observing and rating the degree of infection of  Peronospora belbahrii  disease development by evaluating the level of sporulation on the lower surface of each leaf of the plant.

Metadata:
- Claim Count in Document: 83.0
- Percentile: 90.0
- Lexical Diversity: 2.27397
- Patent Class: 800.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['15812968', '14609304', '15583303', '15294312', '15294269']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6320862352682107
- 35 USC 102 Novelty (BERT): 0.5281459181817824
- Combined Prediction Score: 0.6216922035595679
- Mean Citation Score: 302.10578
- Max Citation Score: 386.0843
- Similarity Product: 312.23864871218206

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test