PATENT CLAIM ANALYSIS

Application Number: 16058646
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-12
Patent Classification: ["205", "777500"]

Abstract:
The present invention relates to a nucleic acid sequencing method that determines the sequence of a template nucleic acid by electrochemical means via the depletion of metal ions as a result of its binding to and/or precipitation with pyrophosphate.

Claim (Index 19):
An electrochemical nucleic acid sequencing method comprising the steps of:\n a. providing a sample comprising a template nucleic acid to be sequenced; b. annealing a primer molecule to said template nucleic acid, or creating a nick in the non-template strand for a site-specific elongation; c. carrying out nucleic acid elongation steps with a polymerase on said template in the presence of metal ions, wherein each step comprises the addition of a single type of nucleotide, wherein pyrophosphate is released in a stoichiometric ratio to the number of nucleotides incorporated, and wherein the metal ions are capable of binding to pyrophosphate in solution; d. determining in said sample the potential, current or charge at an electrode at least twice, wherein between the first time and the second time one or more elongation steps take place or, alternatively, determining the potential, current or charge continuously; and e. correlating the change in potential, current or charge by using a potentiometer, wherein the working electrode comprises manganese dioxide, over the number of elongation steps with the type of nucleotides added.

Metadata:
- Claim Count in Document: 17.0
- Percentile: 96.0
- Lexical Diversity: 1.25
- Patent Class: 205.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14237328', '14860553', '13434627', '13903734', '11271678']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7464801421652171
- 35 USC 102 Novelty (BERT): 0.4954673214134494
- Combined Prediction Score: 0.7213788600900404
- Mean Citation Score: 230.32877
- Max Citation Score: 243.715
- Similarity Product: 177.06621914029122

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test