PATENT CLAIM ANALYSIS

Application Number: 16148783
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-03
Patent Classification: ["536", "023100"]

Abstract:
Isolation or in vitro assembly of the Cas9-crRNA complex of the  Streptococcus thermophilus  CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule using an RNA-guided DNA endonuclease comprising at least one RNA sequence and at least one clan RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro or using a cassette containing a single repeat-spacer-repeat unit.

Claim (Index 52):
A method of preparing a Cas9-crRNA complex for modification of a heterologous target DNA molecule, the method comprising:\n contacting a Cas9 polypeptide with a crRNA and a tracrRNA in vitro to form the Cas9-crRNA complex, wherein the crRNA is heterologous to the Cas9 polypeptide and engineered to bind to the Cas9-crRNA complex to the target DNA molecule; and contacting the target DNA molecule with the Cas9-crRNA complex, whereby the Cas9-crRNA complex modifies the target DNA molecule.

Metadata:
- Claim Count in Document: 74.0
- Percentile: 97.0
- Lexical Diversity: 1.89041
- Patent Class: 536.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14385241', '15507176', '15032985', '14836753', '14942782']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.3612100457349448
- 35 USC 102 Novelty (BERT): 0.7762744578033222
- Combined Prediction Score: 0.4027164869417825
- Mean Citation Score: 585.599808
- Max Citation Score: 975.8007
- Similarity Product: 920.9043727968692

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test