PATENT CLAIM ANALYSIS

Application Number: 16198902
Application Type: Utility
Filing Date: 2018-11
Publication Date: 2019-03
Patent Classification: ["435", "006120"]

Abstract:
The present invention provides methods, compositions and kits for enriching and determining nucleotide sequences of a plurality of target loci from a sample comprising nucleic acids. The methods comprise one or more cycles of primer extension followed by PCR amplification of target sequences using nested target-specific primers.

Claim (Index 25):
A kit comprising:\n (a) a universal adaptor, wherein the universal adaptor is an oligonucleotide comprising a ligatable duplex portion at a first end and a non-duplex portion at a second end; (b) a universal adaptor primer, wherein the universal adaptor primer is capable of annealing to a complementary sequence of the non-duplex portion of the universal adaptor; (c) an outside primer; and (d) an inside primer; wherein the inside primer is nested with respect to the outside primer for a locus of interest, optionally, wherein: (1) the universal adaptor or the 5\u2032 end of the universal adaptor primer comprises a sequence that is identical or complementary to the sequence of a first sequencing primer compatible with an NGS platform, for example,\n (i) the inside primer comprises a sequence identical or complementary to the sequence of a second sequencing primer compatible with the NGS platform; and/or \n (ii) the kit further comprising a sequence adaptor primer comprising at the 3\u2032 end a sequence identical to the inside primer and at the 5\u2032 end a sequence identical or complementary to the sequence of a second sequencing primer compatible with the NGS platform; \n (2) the kit comprising a plurality of sets of outside primer and inside primer; (3) the kit is used for diagnosis of a cancer, for example, the cancer is lung cancer, breast cancer, or colorectal cancer; and/or (4) the locus of interest is located in any one or more of the genes selected from the group consisting of ALK, BRAF, EGFR, ERBB2, HRAS, KDR, KIT, KRAS, MET, NRAS, NTRK1, PDGFRA, PIK3CA, PTEN, RET, ROS1, and TP53.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 98.0
- Lexical Diversity: 1.25
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['14463498', '13793564', '15269448', '12069174', '14300048']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5625404202533575
- 35 USC 102 Novelty (BERT): 0.5323807732250654
- Combined Prediction Score: 0.5595244555505283
- Mean Citation Score: 339.0472660000001
- Max Citation Score: 356.35602
- Similarity Product: 258.94317061165094

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test