PATENT CLAIM ANALYSIS

Application Number: 16114424
Application Type: Utility
Filing Date: 2018-08
Publication Date: 2018-12
Patent Classification: ["435", "006100"]

Abstract:
The invention provides an exonuclease or an enzymatically active fragment thereof, said exonuclease having the amino acid sequence of SEQ ID NO:1 or an amino acid sequence which is at least about 50% identical thereto, wherein said exonuclease or enzymatically active fragment thereof (i) is substantially irreversibly inactivated by heating at a temperature of about 55° C. for 10 minutes in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25° C., 50 mM KCl and 5 mM MgCl 2 ; (ii) is substantially specific for single stranded DNA; and (iii) has a 3′-5′ exonuclease activity. The invention further provides a method of removing single stranded DNA from a sample, a method of nucleic acid amplification, a method of reverse transcription and a method of nucleic acid sequence analysis in which the exonuclease or enzymatically active fragment thereof is used. The invention still further provides nucleic acids encoding said exonuclease or an enzymatically active fragment thereof and kits or compositions comprising the same.

Claim (Index 8):
A method of nucleic acid sequence analysis, said method comprising a step of sample preparation prior to the analysis step(s), said step of sample preparation comprising contacting the sample to be analysed with an exonuclease or enzymatically active fragment thereof under conditions which permit the digestion of at least a portion of any single stranded DNA present in the sample and optionally then heating the mixture to inactivate said exonuclease or enzymatically active fragment thereof, wherein said exonuclease has the amino acid sequence of SEQ ID No. 1 or an amino acid sequence which is at least about 85% identical thereto, wherein said enzymatically active fragment of said exonuclease is at least about 85% identical to the amino acid sequence of said exonuclease, wherein said exonuclease or enzymatically active fragment thereof\n (i) is at least 90% irreversibly inactivated by heating at a temperature of about 55\u00b0 C. for 10 mins in a buffer consisting of 10 mM Tris-HCl, pH 8.5 at 25\u00b0 C., 50 mM KCl and 5 mM MgCl 2 ; (ii) has activity against double stranded DNA that is equal to or less than 15% of its activity against an equivalent amount of single stranded DNA under the same assay conditions; and (iii) has a 3\u2032-5\u2032 exonuclease activity.

Metadata:
- Claim Count in Document: 14.0
- Percentile: 96.0
- Lexical Diversity: 2.05682
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['15504507', '12840552', '14036459', '09896923', '11435018']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5554751559988482
- 35 USC 102 Novelty (BERT): 0.6763596945099551
- Combined Prediction Score: 0.5675636098499589
- Mean Citation Score: 378.117182
- Max Citation Score: 701.82367
- Similarity Product: 648.1237440932811

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test