PATENT CLAIM ANALYSIS

Application Number: 15923954
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-07
Patent Classification: ["435", "006000"]

Abstract:
In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

Claim (Index 1):
A method for detecting a first allelic variant of a target sequence in a nucleic acid sample suspected of comprising at least a second allelic variant of the target sequence, comprising:\n a) forming a first reaction mixture by combining:\n i. the nucleic acid sample; \n ii. a first allele-specific primer, wherein an allele-specific nucleotide portion of the first allele-specific primer is complementary to the first allelic variant of the target sequence; \n iii. a first allele-specific blocker probe that is complementary to a region of the target sequence comprising the second allelic variant, wherein said region encompasses a position corresponding to the binding position of the allele-specific nucleotide portion of the first allele-specific primer, and wherein the first allele-specific blocker probe comprises a minor groove binder; \n iv. a first locus-specific primer that is complementary to a region of the target sequence that is 3\u2032 from the first allelic variant and on the opposite strand; and \n v. a first detector probe; \n b) carrying out an amplification reaction on the first reaction mixture using the first locus-specific primer and the first allele-specific primer to form a first amplicon; and c) detecting the first amplicon by detecting a change in a detectable property of the first detector probe, thereby detecting the first allelic variant of the target gene in the nucleic acid sample.

Metadata:
- Claim Count in Document: 283.0
- Percentile: 90.0
- Lexical Diversity: 1.69118
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12641321', '14445046', '14856448', '14182718', '14397405']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6425142380858508
- 35 USC 102 Novelty (BERT): 0.6433683267650584
- Combined Prediction Score: 0.6425996469537716
- Mean Citation Score: 518.833392
- Max Citation Score: 610.2238
- Similarity Product: 586.26057588408

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test