PATENT CLAIM ANALYSIS

Application Number: 16147865
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-04
Patent Classification: ["435", "285200"]

Abstract:
In an illustrative embodiment, automated multi-module cell editing instruments comprising one or more flow-through electroporation devices or modules are provided to automate genome editing in live cells.

Claim (Index 1):
An automated multi-module cell editing instrument comprising:\n a housing configured to house all of some of the modules; a receptacle configured to receive cells; one or more receptacles configured to receive nucleic acids; a flow-through electroporation (FTEP) module configured to introduce the nucleic acids into the cells; wherein the FTEP module comprises:\n a. one or more inlets and an inlet channel for introducing a fluid comprising cells and exogenous material into the FTEP module; \n b. an outlet and an outlet channel for removing a fluid comprising transformed cells and exogenous material from the FTEP module; \n c. a flow channel intersecting and positioned between a first inlet channel and the outlet channel, wherein the flow channel decreases in width between the first inlet channel and the center of the flow channel and the outlet channel and the center of the flow channel; and \n d. two or more electrodes positioned in the flow channel between the intersection of the flow channel with the first inlet channel and the intersection of the flow channel with the outlet channel; wherein the electrodes are in fluid communication with fluid in the flow channel but are not in a direct flow path of the cells in the flow channel; and wherein the electrodes apply one or more electric pulses to the cells in the fluid as they pass through the flow channel, thereby introducing the exogenous material into the cells in the fluid; \n a recovery module configured to allow the cells to recover after cell transformation in the FTEP module; an editing module configured to allow the introduced nucleic acids to edit nucleic acids in the cells; a processor configured to operate the automated multi-module cell editing instrument based on user input and/or selection of a pre-programmed script; and an automated liquid handling system to move liquids from the cell receptacle to the growth module, from the growth module to the FTEP module, and from the FTEP module to the editing module, as well as from the one or more nucleic acid receptacles to the FTEP module, all without user intervention.

Metadata:
- Claim Count in Document: 48.0
- Percentile: 97.0
- Lexical Diversity: 1.07407
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['16147871', '16147120', '16147353', '16024816', '15802174']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.609572212388312
- 35 USC 102 Novelty (BERT): 0.5603193284792524
- Combined Prediction Score: 0.6046469239974062
- Mean Citation Score: 345.5263280000001
- Max Citation Score: 442.04102
- Similarity Product: 395.3386187390971

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test