PATENT CLAIM ANALYSIS

Application Number: 16144059
Application Type: Utility
Filing Date: 2018-09
Publication Date: 2019-01
Patent Classification: ["435", "007400"]

Abstract:
The present invention pertains to a method for the generation of neurotoxin-sensitive, neuronal differentiated cells comprising the steps of: a) cultivating tumor cells which are able to differentiate into neuronal cells in a culture medium under conditions and for a time which primes said tumor cells for neuronal differentiation; and b) cultivating the tumor cells primed for neuronal differentiation of a) in a differentiation medium having an osmolality of 100 to 270 mOsm/kg, and comprising (i) B27 supplement and/or (ii) N2 supplement, for at least 3 days, thereby obtaining neurotoxin-sensitive, neuronal differentiated cells. The invention further relates to neurotoxin-sensitive, neuronal differentiated cells obtainable by the method of the invention. In addition, the invention encompasses a method for determining the activity of a neurotoxin polypeptide comprising the steps of: a) contacting the neurotoxin-sensitive, neuronal differentiated cells obtainable by the method of the invention with a neurotoxin polypeptide; b) cultivating the neurotoxin-sensitive, neuronal differentiated cells of step a) for 3 to 74 hours or 72 hours under conditions which allow for the neurotoxin polypeptide to exert its biological activity; and c) determining the activity of the neurotoxin polypeptide in the said cells after cultivation according to step b). Finally, the invention provides for a medium comprising OptiMEM, FBS, B27 supplement, and N2 supplement.

Claim (Index 13):
The method of  claim 9 , wherein the SiMa or P19 tumor cells are cultivated in step a) on tissue culture dishes which are coated with at least one compound selected from the group consisting of poly-L-lysine, poly-D-lysine, collagen, laminins, and gelatin.

Metadata:
- Claim Count in Document: 29.0
- Percentile: 97.0
- Lexical Diversity: 2.72222
- Patent Class: 435.0
- Transitional Phrase Type: closed
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14435583', '13779972', '15528219', '13829783', '14214598']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.647395908642392
- 35 USC 102 Novelty (BERT): 0.5992508381750493
- Combined Prediction Score: 0.6425814015956578
- Mean Citation Score: 319.7465880000001
- Max Citation Score: 632.563
- Similarity Product: 439.2016845985055

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test