PATENT CLAIM ANALYSIS

Application Number: 16169376
Application Type: Utility
Filing Date: 2018-10
Publication Date: 2019-02
Patent Classification: ["435", "188000"]

Abstract:
A series of protein A mutants having high alkali resistance, and methods of using the protein A mutants are provided. The protein A mutants have a high binding affinity for regions of immunoglobulin proteins other than the complementarity determining regions. The protein A mutants can be coupled to a solid support for immunoglobulin isolation, or conjugated to a label for immunoglobulin detection. This series of protein A mutants have high chemical stability under alkaline conditions of pH 13-14, and can also be used as chromatography ligands for purification procedures that use alkaline solutions under harsh conditions, such as Clean-In-Place (CIP). Also provided are methods of immunoglobublin separation and purification, and alkali regeneration of affinity chromatography medium that uses protein A as a ligand.

Claim (Index 27):
A method of purifying an immunoglobulin, the method comprising:\n (i) contacting a sample comprising the immunoglobulin with a multimer of  claim 17  immobilized to a solid support to capture the immunoglobulin with the protein A mutant; and (ii) eluting the captured immunoglobulin from the multimer immobilized to the solid support to obtain purified immunoglobulin.

Metadata:
- Claim Count in Document: 23.0
- Percentile: 97.0
- Lexical Diversity: 1.875
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['14778395', '14845817', '14961164', '12280221', '12900958']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6355755761305951
- 35 USC 102 Novelty (BERT): 0.5770316204681408
- Combined Prediction Score: 0.6297211805643497
- Mean Citation Score: 326.258792
- Max Citation Score: 517.2193
- Similarity Product: 385.5472953707636

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test