PATENT CLAIM ANALYSIS

Application Number: 15765742
Application Type: Utility
Filing Date: 2018-04
Publication Date: 2019-01
Patent Classification: ["506", "016000"]

Abstract:
The present invention relates to a method of producing a set of primers suitable for the reverse transcription and/or amplification of a plurality (N) of nucleic acid molecules of interest, wherein for each nucleic acid molecule of interest at least one primer is produced and wherein the primers carry a bar-code, the method comprising the steps of: (a)(i) combining (1) a first oligonucleotide, wherein said first oligonucleotide comprises a first bar-code nucleic acid sequence linked at its 3′ end to a first adapter nucleic acid sequence with (2) a plurality (N) of second oligonucleotides, wherein each second oligonucleotide comprises the reverse complementary sequence of a forward primer specific for a nucleic acid molecule of interest, wherein said reverse complementary sequence of the forward primer is linked at its 3′ end to the reverse complementary sequence of the first adapter nucleic acid sequence; and/or (a)(ii) combining (1) a third oligonucleotide, wherein said third oligonucleotide comprises a second bar-code nucleic acid sequence linked at its 3′ end to a second adapter nucleic acid sequence with (2) a plurality (N) of fourth oligonucleotides, wherein each fourth oligonucleotide comprises the reverse complementary sequence of a reverse primer specific for said nucleic acid molecule of interest, wherein said reverse complementary sequence of the reverse primer is linked at its 3′ end to the reverse complementary sequence of the second adapter nucleic acid sequence; wherein steps (a)(i) and (a)(ii) are carried out under conditions that enable the annealing of the first and second adapter nucleic acid sequences to the respective reverse complementary sequences thereof; (b) extending the oligonucleotides of (a)(i) and (a)(ii) by polymerase-mediated oligonucleotide synthesis; and (c) optionally, removing the second and fourth oligonucleotides. The present invention further relates to methods of producing a plurality (M) of nucleic acid amplification products of interest carrying at least one sample-specific bar-code as well as to a method for multiplex sequencing of a plurality (M) of nucleic acid amplification products of interest from a plurality (X) of samples in a single reaction chamber and identifying the individual sample from which each nucleic acid amplification product is derived. Furthermore, the present invention relates to a target-unspecific bar-code-adapter panel, its use in the methods of the invention as well as a kit comprising same.

Claim (Index 11):
A method for multiplex sequencing of a plurality (M) of nucleic acid amplification products and/or reverse transcription products of interest from different samples and identifying the individual sample from which each nucleic acid amplification product and/or reverse transcription product is derived, the method comprising:\n (a) producing a plurality (M) of nucleic acid amplification products and/or reverse transcription products of interest for each individual sample in accordance with the method of  claim 9 ; (b) combining the nucleic acid amplification products and/or reverse transcription products produced in (a) from all samples; (c) sequencing the combined nucleic acid amplification products and/or reverse transcription products of (b); and (d) identifying the individual sample from which each nucleic acid amplification product and/or reverse transcription product is derived based on the sample-specific bar-code associated with the nucleic acid amplification product and/or reverse transcription product.

Metadata:
- Claim Count in Document: 2.0
- Percentile: 91.0
- Lexical Diversity: 3.85345
- Patent Class: 506.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: True
- Related Applications: ['11791135', '13264122', '15127672', '14600550', '14586857']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.5806557437693517
- 35 USC 102 Novelty (BERT): 0.5257769348692046
- Combined Prediction Score: 0.575167862879337
- Mean Citation Score: 312.6286100000001
- Max Citation Score: 319.8021
- Similarity Product: 226.38044953465464

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 0
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 0

Dataset: test