PATENT CLAIM ANALYSIS

Application Number: 15896423
Application Type: Utility
Filing Date: 2018-02
Publication Date: 2018-08
Patent Classification: ["424", "199100"]

Abstract:
Disclosed are compositions and methods of treating a neurodegenerative disease in an individual. The methods disclose administration of an Integrin α4β1, Very Late Antigen-4 positive neural precursor cell (“VLA4+ NPC”) transfected with a lentivirus overexpressing wild type GCase to an individual having a neurodegenerative disorder. The neurodegenerative disease may include lipid storage diseases, for example Gaucher disease, Parkinson's disease (PD), Dementia with Lewy bodies.

Claim (Index 18):
The method of  claim 16 , wherein said one or more CRISPR corrected genes is achieved by the steps of\n a. contacting an iPSC derived from said individual with a guide RNA (\u201cgRNA\u201d) specific for a targeted genomic region containing said one or more mutations and a CRISPR-associated endonuclease (preferably Cas9,  S. pyogenes  Cas9 (SpCas9), Cpf1, or the like) with in an amount and for a duration sufficient to convert said mutation to a wild-type sequence; b. assessing CRISPR-Cas9 cleavage activity; c. genotyping edited human iPSC cell clones d. functionally screening said edited human iPSC cell clones via an enzyme activity assay; e. karyotyping for chromosomal analysis of said edited human iPSC cell clones; f. differentiating said iPSC cells to neural precursor cells (NPCs); g. deriving a VLA4+ NPC enriched population from said NPCs, preferably wherein said step of deriving said VLA4+ NPC population is carried out by subjecting said NPCs to a FACS sorting step.

Metadata:
- Claim Count in Document: 31.0
- Percentile: 88.0
- Lexical Diversity: 1.36842
- Patent Class: 424.0
- Transitional Phrase Type: none
- Component Type: 0
- Foreign Priority: False
- Related Applications: ['15399165', '14494065', '14127546', '14884503', '12384446']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7304038601198073
- 35 USC 102 Novelty (BERT): 0.5114873380782153
- Combined Prediction Score: 0.7085122079156481
- Mean Citation Score: 221.888356
- Max Citation Score: 252.54108
- Similarity Product: 157.80379481280326

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test