PATENT CLAIM ANALYSIS

Application Number: 15939231
Application Type: Utility
Filing Date: 2018-03
Publication Date: 2018-11
Patent Classification: ["435", "220000"]

Abstract:
The present invention relates to the fields of collagenase production and collagenase products, and particularly to improving the reproducibility, purity, and stability of collagenase I and collagenase II compositions, where the compositions are pure to at least 95% by area as measured by reverse phase high pressure liquid chromatography (RP-HPLC) and essentially free of neutral protease.

Claim (Index 54):
A process for producing isolated and purified collagenase I obtained or derived from  C. histolyticum , comprising the steps of:\n a. fermenting  C. histolyticum;  b. harvesting a crude fermentation comprising collagenase I and collagenase II; c. purifying collagenase I and collagenase II comprising the steps of:\n i. filtering the crude fermentation over an anion exchange filter; \n ii. precipitating the filtrate from step (c)(i) with ammonium sulfate comprising less than about 80 ppm zinc and less than about 1.2 ppm nickel; \n iii. resuspending the precipitated product from step (c)(ii); \n iv. subjecting the resuspension from step (c)(iii) to a hydrophobic interaction chromatography column; \n v. separating the collagenase I and collagenase II from the filtrate of step (c)(iv) using ion-exchange chromatography; and \n vi. collecting the filtrate of step (c)(v) in fractions; \n d. estimating the total amount of collagenase II collected in each fraction from step (c)(vi); e. estimating the amount of collagenase I collected in each fraction from step (c)(vi); and f. pooling fractions containing collagenase I from the peak of collagenase I in the fractions towards the tail of the collagenase I in the fractions, until the amount pooled of collagenase I is about the same as the amount of collagenase II estimated in step (d).

Metadata:
- Claim Count in Document: 26.0
- Percentile: 90.0
- Lexical Diversity: 1.3913
- Patent Class: 435.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['12474488', '13515203', '11699302', '13422939', '14394481']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.6296780344902836
- 35 USC 102 Novelty (BERT): 0.5173666046956732
- Combined Prediction Score: 0.6184468915108225
- Mean Citation Score: 300.109898
- Max Citation Score: 338.7013
- Similarity Product: 289.52473317967656

Labels:
- Claim Label 101: 1
- Claim Label 102: 1
- Claim Label 103: 1
- Claim Label 112: 1
- Combined Label: 1
- Label 101 Adjusted: 1

Dataset: test