PATENT CLAIM ANALYSIS

Application Number: 16045539
Application Type: Utility
Filing Date: 2018-07
Publication Date: 2019-06
Patent Classification: ["424", "136100"]

Abstract:
Described herein are methods for the efficient production of antibodies and other multimeric protein complexes (collectively referred to herein as heteromultimeric proteins) capable of specifically binding to more than one target. The targets may be, for example, different epitopes on a single molecule or located on different molecules. The methods combine efficient, high gene expression level, appropriate assembly, and ease of purification for the heteromultimeric proteins. The invention also provides methods of using these heteromultimeric proteins, and compositions, kits and articles of manufacture comprising these antibodies.

Claim (Index 1):
A method of preparing a heteromultimeric protein comprising a first hinge-containing polypeptide having a first heterodimerization domain and a second hinge-containing polypeptide having a second heterodimerization domain, wherein the second heterodimerization domain interacts with the first heterodimerization domain, and wherein the first and second hinge-containing polypeptides are linked by at least one interchain disulfide bond, the method comprising the steps of:\n (a) culturing a first host cell comprising a first nucleic acid encoding the first hinge-containing polypeptide under conditions where the hinge-containing polypeptide is expressed; (b) culturing a second host cell comprising a nucleic acid encoding the second hinge-containing polypeptide under conditions where the hinge-containing polypeptide is expressed; (c) disrupting the cell membranes of the first and second host cells, wherein the first and second host cells have been combined together in a single suspension; and (d) recovering the heteromultimeric protein, wherein said method does not require the addition of a reductant.

Metadata:
- Claim Count in Document: 1.0
- Percentile: 95.0
- Lexical Diversity: 1.52308
- Patent Class: 424.0
- Transitional Phrase Type: open
- Component Type: 1
- Foreign Priority: False
- Related Applications: ['13092708', '13958318', '14735024', '15789775', '14251428']

Analysis Scores:
- 35 USC 101 Eligibility (BERT): 0.7000352602167287
- 35 USC 102 Novelty (BERT): 0.5693727489597032
- Combined Prediction Score: 0.6869690090910262
- Mean Citation Score: 369.833202
- Max Citation Score: 505.7528
- Similarity Product: 355.74538200836184

Labels:
- Claim Label 101: 1
- Claim Label 102: 0
- Claim Label 103: 1
- Claim Label 112: 0
- Combined Label: 0
- Label 101 Adjusted: 1

Dataset: test