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 D153489_sort CYTOMETER INFO User Name: Experiment Name:Administrator Administrator 20231005_Sort_FRM0535 cells Application Name: Application Data Version:BD FACSChorus 1. 4. 3. 0Cytometer Serial Number: Cytometer Name:R6643410023 FACSMelody SORT DETAILS Sort Mode: Sort Device: Total Events: Processed Events:Purity 2-Way Tubes 5. 0m L 11,878,643 97. 9%Sort Status: Nozzle Size: Pressure: Drop Frequency:Stopped by System 100 micron 22. 92 PSI 34. 0 k Hz Start Date Time: End Date Time:10/05/2023 02:50PM 10/05/2023 03:14PM  SORT STATISTICS Tube Population Target Count Sort Count Sort Rate Efficiency Time 1 Pos fraction 1,281,600 630,452 431 62% 24m 20s 2Neg fraction 1,296,000 151,015 103 60% 24m 18s CYTOMETER SETTINGS Fluorochrome PM T Voltages FSC 28 PE (YG) 463 APC 522 APC-Cy7 531 SSC 384 BB515 465 Per CP-Cy5. 5 547 Threshold:FSC @ 10000 Compensation: Spillover Values Into (Detectors)From (Fluorochromes) PE (YG) APC APC-Cy7 BB515 Per CP-Cy5. 5 PE (YG) 100. 00 2. 99 0. 00 0. 00 0. 00 APC 0. 00 100. 00 3. 83 0. 00 0. 62 APC-Cy7 0. 00 14. 05 100. 00 0. 00 12. 41 BB515 0. 00 0. 00 0. 00 100. 00 0. 00 Per CP-Cy5. 5 21. 65 1. 61 0. 00 2. 09 100. 00 POPULATION HIERARCHY All Events Scatter SSC Singlets FSC Singlets P1 P2 Pos fraction Neg fraction

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