Patent Document ID: 9972087
Application ID: 15325355
Patent Status: 1

Claim One:
1. A quantitative determination method of a biological substance in a sample stained with a staining reagent comprising fluorescent particles each encapsulating a fluorescent substance and binding to a biological substance recognizing site, the method comprising: an inputting step of inputting a fluorescent image obtained by photographing the sample, a first calculating step of extracting a predetermined region from the fluorescent image to calculate an integrated luminance of the predetermined region, and a second calculating step of counting the number of fluorescent particles contained in the predetermined region from the integrated luminance and the average luminance per fluorescent particle, wherein the average luminance per fluorescent particle is calculated in advance from a correlation between the number of fluorescent particles actually counted from an image taken with an electron microscopic and the luminance derived from fluorescent light from the fluorescent particles and calculated from a fluorescent image of a region identical to the region taken in the image taken with an electron microscopic from which the number of fluorescent particles is actually counted.