Patent Document ID: 8912393
Application ID: 13220570
Patent Status: 1

Claim One:
1. A maize activation tagging DNA construct comprising: a coding sequence for a transposase; a detectable reporter encoding region comprising: a sequence encoding anthocyanin regulatory gene B-Peru operably linked to a first constitutive promoter; and a sequence encoding anthocyanin regulatory gene C1 operably linked to a second constitutive promoter; and a non-autonomous transposable T-DNA cassette inserted into the detectable reporter encoding region such that the B-Peru and C1 genes induce expression of anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette, the transposable cassette comprising: a pair of DNA substrates for the transposase, having disposed therebetween: a transcriptional enhancer element comprising at least two copies of a Sugar Cane Bacilliform Virus (SCBV) enhancer; and, optionally a sequence encoding a selectable marker operably linked to the transcriptional enhancer element.