Patent Document ID: 20030175720
Application ID: 10100262
Patent Status: 0

Claim One:
1. A method of determining the relative abundance of genetic material within a first sample and a second sample, comprising the steps of: labeling the first sample with a first detectable label adapted to bind with genetic material therein; labeling the second sample with a second detectable label adapted to bind with genetic material therein; providing a substrate with a binding area thereon for hybridizing with a genetic sequence; contacting the substrate with the first and second samples; obtaining a digital image of the binding area under conditions causing said labels to manifest an image intensity dependent on the abundance of genetic material bound to the binding area, thus providing an array of pixel values comprising respective intensity values corresponding the first and second labels; applying a clustering procedure to the array of pixel values to obtain (a) a background medoid vector having coordinates of a first label intensity background value and a second label intensity background value, and (b) a foreground medoid vector having coordinates of a first label intensity foreground value and a second label intensity foreground value; and providing a measure of the relative abundance of genetic material within the first and second samples corresponding to the binding area based on a ratio of differences between the respective coordinates of the background and foreground medoid vectors.