Patent Document ID: 10068126
Application ID: 15045556
Patent Status: 1

Claim One:
1. A method for relocating an object of interest at a second imaging station based on coordinates for the object of interest at a first imaging station, the method comprising: acquiring a first pair of images including a reference image of a biological specimen on the first imaging station and a second image of the biological specimen on the second imaging station, the biological specimen including cellular content deposited on a slide, and the reference image being selected from a plurality of reference image candidates based on a suitability score assigned to the reference image candidates, wherein the suitability score of each reference image candidate is indicative of an amount of the cellular content in the corresponding reference image candidate; identifying a first cell group that appears in both the reference image and second image; determining stage coordinates of the first cell group on the first imaging station from stage coordinates at which the reference image was acquired; determining stage coordinates of the first cell group on the second imaging station from stage coordinates at which the second image was acquired; calculating an affine transformation that converts the stage coordinates of the first imaging station to the stage coordinates of the second imaging station; acquiring at least a second pair of images including a second reference image acquired at the first imaging station and a third image acquired at the second imaging station; for the at least second pair of images, identifying a second cell group that appears in both the reference image and the third image; determining the stage coordinates of the second cell group associated with the at least second pair of images on the first imaging station and the second imaging station; and calculating the affine transformation based on the first and the at least second pairs of images.