Patent Document ID: 20100303386
Application ID: 12791997
Patent Status: 0

Claim One:
1. A method for analyzing a field-of-view of independently blinking objects, comprising the steps of: i) selecting a sample or an observation image comprised of objects from the class of optically signaling objects that stochastically and independently fluctuate; ii) acquiring a sequence of repeated optical images of the object as a function of time, producing an x, y, t image stack of pixels; iii) extracting a time trajectory for each pixel from said image stack; iv) calculating an autocorrelation or autocummulant of each pixel's time trajectory to at least the second order, and v) generating a superresolved and background-reduced image from the calculated functions comprising pixels whose intensity value is the amplitude, with a chosen time lag, of the autocorrelation or autocummulant function, or the value of a finite integral of the autocorrelation or autocummulant function over a range of time lags.