Patent Document ID: 9658442
Application ID: 14126712
Patent Status: 1

Claim One:
1. A method for imaging and analyzing an object labelled with stochastically and independently blinking point-like emitters, said method comprising the following steps: acquiring a time series of images from blinking point-like emitters, calculating higher-order cumulants from said acquired images from the blinking point-like emitters, determining the parameter maps for molecular state lifetimes, amplitude and concentration of said emitters by establishing a multi-state blinking model, expressing several cumulant orders as a function of parameters involved in the parameter maps and solving resulting equations for the parameters, obtaining a balanced cumulant by spatially deconvolving the cumulant of order n, by taking then the n-th root and by compensating for weighting factors using the parameter maps of the step of determining, and displaying said parameter maps and balanced cumulants to obtain information about structural and/or functional features of the object identified by said blinking point-like emitters.