Patent Document ID: 8060348
Application ID: 11606582
Patent Status: 1

Claim One:
1. A system for analyzing tissue samples, comprising, a storage device for at least temporarily storing one or more images of one or more cells in one or more of the tissue samples, wherein said images comprise a plurality of channels; a processor that quantifies a distribution of a biomarker in a plurality of subcellular regions to quantify translocation of the biomarker from at least one subcellular region to another subcellular region, at least in part by using a correlation between at least one image comprising a biomarker channel and at least one image comprising a morphological channel and a ratio of intensity of said biomarker in a membrane subcellular region and another subcellular region; and a score corresponding to the quantified translocation; and a display device for displaying one or more of the images, the score, or both, wherein the ratio of intensity is an average intensity of said biomarker in a membrane subcellular region and another subcellular region and, wherein said correlation is r and, r = âˆ‘ i â¢ ( A i - A _ ) â¢ ( B i - B _ ) âˆ‘ i â¢ ï˜ƒ A i - A _ ï˜„ 2 â¢ âˆ‘ i â¢ ï˜ƒ B i - B _ ï˜„ 2. wherein A i is an intensity of a pixel on the image comprising the biomarker channel, Ä€ is an average of the intensity of all the pixels on the image comprising the biomarker channel, B i is an intensity of a pixel on the image comprising the morphological channel, B is an average of the intensity of all the pixels on the image comprising the morphological channel .