Patent Document ID: 8121383
Application ID: 12889496
Patent Status: 1

Claim One:
1. A computer product for automated digital image analysis of cells, the computer product comprising a processor and memory, the computer product programmed to: compute a plurality of total intensities x i for each cell of a plurality of cells using a set of rings of a pre-determined size to create a radial distribution for the cell with respect to a nuclear boundary of the cell; derive a nuclear translocation measurement for each cell from the radial distribution of the cell by using a weighted sum of the total intensities x i of the cell by computing: translocation = 1 n - 2 + n - 1 ⁢ x - 2 + 1 n - 2 + n - 1 ⁢ x - 1 - 1 n 2 ⁢ x 2 , where x −i comprises a total intensity x at index i and where n −k is a number of pixels comprising a mask at index k; and create an automated analysis protocol for digital image analysis using the nuclear translocation measurements derived for all of the cells.