Patent Document ID: 9784665
Application ID: 14983296
Patent Status: 1

Claim One:
1. A method for scoring muscular dystrophy-linked protein status in muscle tissue using digital image analysis, comprising: a. obtaining one or more muscle biopsy tissue samples from patients submitted for evaluation; b. processing said one or more samples to produce one or more stained tissue sections mounted on one or more glass slides; c. digitizing said one or more slides using a digital slide scanner or a microscope coupled with a camera to generate digital images corresponding to each of the tissue sections; d. displaying said digital images of said one or more stained tissue sections for visualization and review by a user; e. applying an algorithm process implemented by a computer to said digital images of the one or more stained tissue sections to extract staining and morphometric features of each muscle fiber within the images; f. for each images of the stained tissue sections, displaying results of said algorithm process overlaid on the corresponding image; g. combining one or more staining and morphometric feature parameters to derive a score of disease status; h. displaying said score in a graphical manner using a graphical user interface for user visualization and assessment; and i. interpreting the score of disease status to draw inferences associated with the severity of disease in each sample submitted for evaluation.