Patent Document ID: 9569828
Application ID: 13661364
Patent Status: 1

Claim One:
1. A method for the imaging of labeled biological samples labeled with detectable labels, using fluctuating intensities of radiated light from the biological samples, and comprising: illuminating the labeled biological samples to produce the fluctuating intensities of radiated light in the labeled biological samples; and localizing the detectable labels, the localizing comprising: repeatedly collecting light scattered, reflected and fluoresced from the labeled biological sample with a detection device, thereby generating members of a time series of images of the labeled biological samples, wherein at least some of the time series of images illustrate the fluctuating intensities of radiated light; generating a series of difference images between different pairings of later members of the time series of images and earlier members of the time series of images having the fluctuating intensities of radiated light by subtracting pixel intensities of the earlier members of the time series of images from the later members of the time series of images; filtering the series of difference images with a filter; and subsequently summing the filtered difference images to generate a final image of the labelled biological samples.