Patent Document ID: 8269827
Application ID: 12569396
Patent Status: 1

Claim One:
1. A method for generating a brightfield type image, that resembles a brightfield staining protocol of a biological sample, using fluorescent images comprising the steps of: acquiring, by a digital imaging device, two or more fluorescent images of a fixed area on a biological sample; mapping said fluorescent images into a brightfield color space; and generating a bright field type image; the mapping step comprises: acquiring a bright field image of the fixed area of the biological sample; analyzing the image data of the bright field image and the two or more fluorescent images utilizing, at least in part, featured based information or pixel intensity data information to generate mapping parameters to transform the two or more fluorescent images into a bright field color space; and applying said mapping parameters to the two or more fluorescent images; the generating mapping parameters step comprises: using a linear estimation model; the linear estimation model is defined as: A ^ = arg ⁢ ⁢ min ⁢ A ⁢ ( HE - A · FL ) 2 where Â is the estimated mapping parameters, HE represents intensity values in the color channels of the Hematoxylin & Eosin (H&E) image, and FL is the intensity values of at least two color channels of the fluorescent images.