Patent Document ID: 20120105600
Application ID: 12999515
Patent Status: 0

Claim One:
1. A method for 3D imaging of a biologic object ( 1 ) in an optical tomography system comprising: (a) labeling ( 10 ) the subcellular structure of a biological object ( 1 ) by introducing at least one nanoparticle-biomarker ( 3 ); (b) positioning ( 14 ) the labeled biological object ( 1 ) relatively to a microscope objective ( 62 ) to present varying angles of view; (c) illuminating the labeled biological object ( 1 ) with radiation having wavelengths between 150 nm and 900 nm so that the at least one nanoparticle-biomarker ( 3 ) is selected to emit or absorb radiation having wavelengths between 150 and 900 nm; (d) filtering ( 15 ) a portion of the produced radiation using a set of filters to produce filtered signals within a set of wavelength bands; (e) sensing ( 16 ) the filtered signals transmitted through the labeled biological object ( 1 ) and the microscope objective ( 62 ) within the set of wavelength bands to produce image data; (f) repeating steps (b) through (e) until a set of image data from varying angles of view has been produced; (g) forming ( 26 ) a plurality of cross-sectional images of the biological object ( 1 ) from the set of image data; and (h) reconstructing the plurality of cross-sectional images to form a 3D image of the labeled biological object ( 1 ).