Patent Document ID: 9863864
Application ID: 15264326
Patent Status: 1

Claim One:
1. A flow cytometry apparatus comprising: a detector configured to produce a detector signal responsive to detecting light during operation of the flow cytometer; a first pulse processor configured to receive the detector signal from the detector and to output detector data characterizing pulse heights of pulses in the detector signal; a second pulse processor configured to also receive the detector signal from the detector and to output a pulse height data signal, a pulse width data signal, and a pulse area data signal for desired pulses in the detector signal, wherein, for each desired pulse: the second pulse processor is configured to output the pulse height data signal, the pulse width data signal, and the pulse area data signal for that desired pulse responsive to a set of one or more trigger conditions being met, and the set of one or more trigger conditions at least includes a trigger condition involving a comparison of the pulse height for that desired pulse with a trigger threshold value; a real-time trigger module configured to receive the detector data from the first pulse processor and to generate periodic histogram data from the detector data; and a threshold selector configured to receive the periodic histogram data from the real-time trigger module and to provide the trigger threshold value.