Patent Document ID: 9556428
Application ID: 14718656
Patent Status: 1

Claim One:
1. A method of displaying a multi-chain antibody on the surface of a yeast host cell, comprising: (i) introducing a yeast display vector or a yeast display vector set into a yeast host cell, wherein the yeast display vector or the yeast display vector set collectively comprising (a) a first polynucleotide encoding a polypeptide comprising a heavy chain of a multi-chain antibody; and (b) a second polynucleotide encoding a light chain of the multi-chain antibody; and (ii) culturing the yeast host cell obtained in (i) to allow expression of the heavy and light chains of the multi-chain antibody, wherein the heavy chain and the light chain are expressed as separate polypeptides, and wherein the multi-chain antibody is displayed at the surface of the yeast host cell.