Patent Document ID: 9002077
Application ID: 12854058
Patent Status: 1

Claim One:
1. A method, comprising: applying an immunohistochemical stain, eosin, and a counterstain to a sample; obtaining a plurality of images of the sample, each of the plurality of images corresponding to radiation from the sample in a different wavelength band; decomposing the plurality of images of the sample to obtain component images corresponding to the immunohistochemical stain, eosin, and the counterstain; and generating first and second sample images based on the component images derived from the plurality of images, wherein the first sample image comprises contributions from the component images corresponding to the counterstain and to the immunohistochemical stain, and substantially not from the component image corresponding to eosin; wherein the second sample image comprises contributions from the component images corresponding to the counterstain and to eosin, and substantially not from the component image corresponding to the immunohistochemical stain; and wherein each of the component images that contributes to the first and second sample images corresponds to absorption of radiation by a component of the sample.