Patent Document ID: 9338408
Application ID: 13674424
Patent Status: 1

Claim One:
1. An image obtaining apparatus, comprising: a light source configured to irradiate a biological sample having a fluorescent label with excitation light, the excitation light exciting the fluorescent label; an optical system including an objective lens, the objective lens being configured to magnify an imaging target of the biological sample; an image sensor configured to form an image of the imaging target magnified by the objective lens; a movement controller configured to move a focus position of the optical system in an imaging range including at least a range corresponding to the thickness of the imaging target; and a data processing unit configured to expose the image sensor to light while moving the focus position in a thickness direction and a plane direction perpendicular to the thickness direction at the same time to obtain a fluorescent image of the biological sample and calculate distribution information of the fluorescent label in the thickness direction of the imaging target based on analyzing a shape of the fluorescent label in the fluorescent image.