Patent Document ID: 20140198198
Application ID: 14126712
Patent Status: 0

Claim One:
1. Method for imaging and analysing an object labelled with stochastically and independently blinking point-like emitters, said method comprising the following steps: Acquiring a time series of images from blinking point-like emitters, Calculating higher-order cumulants from said acquired images, Determining at least the parameter maps for molecular state lifetimes, amplitude and concentration of said emitters by establishing a multi-state blinking model, expressing several cumulant orders as a function of the parameters involved and solving the resulting equations for the parameters, Obtaining a balanced cumulant by spatially deconvolving the cumulant of order n, by taking then the n-th root and by compensating for the temporal weighting factors using the previously determined parameter maps, Displaying said parameter maps and balanced cumulants in a way as to obtain information about the structural and/or functional features of the object reported by said emitters.