Patent Document ID: 20120257811
Application ID: 13442852
Patent Status: 0

Claim One:
1. A computerized method for digital analysis of tissue objects, comprising: providing three or more adjacent tissue sections, at least one of said adjacent tissue sections being stained with a biomarker; acquiring a first digital image of a first adjacent tissue section, a second digital image of a second adjacent tissue section, and a third digital image of a third adjacent tissue section; using a computerized software, aligning the first and third digital images with the second digital image such that the first through third digital images are two-dimensionally aligned within said computerized software; overlaying a first digital grid about the first digital image, the first digital grid comprising a plurality of first grid squares; on the first digital image, assigning a first IHC score to each of the plurality of first grid squares within the first digital grid, and summating the first grid squares to generate a first IHC signature score; overlaying a second digital grid about the second digital image, the second digital grid comprising a plurality of second grid squares; on the second digital image, assigning a second IHC score to each of the plurality of second grid squares within the second digital grid, and summating the second grid squares to generate a second IHC signature score; overlaying a third digital grid about the third digital image, the third digital grid comprising a plurality of third grid squares; on the third digital image, assigning a third IHC score to each of the plurality of third grid squares within the third digital grid, and summating the third grid squares to generate a third IHC signature score; and summating the first through third IHC signature scores to generate a numerical representation of a three-dimensional IHC score.