Patent Document ID: 9058648
Application ID: 13803562
Patent Status: 1

Claim One:
1. A method for measuring the chemiluminescence of a sample, comprising: acquiring a binned initial image of the sample, the initial image having a first binning value representing the number of pixel elements in its image pixels; calculating an exposure time for a final image of the sample, the final image having a second binning value representing the number of pixel elements in its image pixels, wherein the first binning value is larger than the second binning value, and wherein the calculation is based on a pixel ratio of the first binning value to the second binning value; acquiring at least first and second final images of the sample, wherein the first final image is acquired using the calculated exposure time, wherein the second final image is acquired using an exposure time shorter than the calculated exposure time; identifying pixels in the first final image that are saturated; measuring the intensity of luminescence of corresponding features in both the first and second final images, wherein measuring provides an intensity ratio that reflects the intensity of features in the first final image in relation to the intensity of the corresponding features in the second final image; substituting, for any saturated pixels in the first final image, corresponding pixels from the second final image; and adjusting the intensity of the corresponding pixels that are substituted for saturated pixels according to the intensity ratio.