Patent Document ID: 9767341
Application ID: 13994052
Patent Status: 1

Claim One:
1. A system for imaging a moving object within a microfluidic environment comprising: a flow cell configured to carry the moving object within a flow of carrier fluid; an illumination source configured to illuminate a first side of the flow cell through a spatial filter interposed between the illumination source and the flow cell; an image sensor disposed on a second, opposite side of the flow cell, the image sensor having an imaging plane that is located at a distance z 2 from the flow cell, the imaging plane defined by an array of pixels in orthogonal x and y directions, and wherein the flow cell is located a distance z 1 from the spatial filter and z 2 <<z 1 , wherein the flow cell is angled relative to an edge of the array of pixels of the image sensor between a non-zero angle and less than 45° so as to generate a shift component on the imaging plane in both the x and y directions; at least one processor configured to receive a plurality of image frames of the moving object from the image sensor, each image frame having a low resolution hologram with sub-pixel shifts in the x and y direction as a function of time, wherein the at least one processor generates a super resolved hologram using the sub-pixel shifted lower resolution holograms; and wherein the at least one processor eliminates a twin image artifact so that the super resolved hologram yields the recovery of a high resolution image of the object.