Patent Document ID: 20140072195
Application ID: 14024474
Patent Status: 0

Claim One:
1. An automated reader for reading fluorescence in-situ hybridization signals, comprising: one or more computer processors for receiving a digitized FISH image comprising a plurality of spots having colors and, the one or more processors being programmed to execute the steps of: converting colors within the FISH image to a hue value; separately for each color: extracting a plurality of quantitative values to detect the presence of signals corresponding to spots; applying a plurality of algorithms to extract features from the signals to determine cell shapes and segment cells within the FISH image; recombining the extracted features for the colors; classifying the plurality of spots according to the color of the spot; separating merged signals of classified spots that are in close proximity to each other within the FISH image; counting the classified spots to determine relative frequency of colors; and generating a report of the number of classified spots of each color.