Patent Document ID: 9696534
Application ID: 14380392
Patent Status: 1

Claim One:
1. A microscopy system for multi-focal structured illumination microscopy comprising: a light source for transmitting a single light beam; a beam splitter for splitting the single light beam into a plurality of light beams forming a multi-focal pattern; a scanner for scanning the plurality of light beams that forms the multi-focal pattern onto a sample such that the sample generates a plurality of fluorescent emissions with each multi-focal pattern; a focusing component defining an aperture configured to block out-of-focus fluorescence emissions of the plurality of fluorescent emissions for each multi-focal pattern and allowing through in-focus fluorescent emissions to pass through the aperture; a scaling component for scaling down the plurality of in-focus fluorescent emissions for each multi-focal pattern, wherein each of the plurality of in-focus fluorescent emissions defines a respective geometric center, and wherein scaling down the plurality of in-focus fluorescent emissions causes each of the in-focus fluorescent emissions to locally contract by a predetermined factor relative to each other such that the distance between each respective geometric center remains the same; a detector for detecting the plurality of scaled in-focus fluorescent emissions that results from each of the multi-focal patterns; and a processor in operative communication with the detector for summing each of the plurality of scaled in-focus fluorescent emissions detected by the detector to produce a plurality of summed, scaled in-focus fluorescent emissions that form a composite image of the plurality of summed, scaled in-focus fluorescent emissions.