Patent Document ID: 20090129660
Application ID: 12253163
Patent Status: 0

Claim One:
1. A method for an automatic detection of cell colonies, comprising: acquiring digital images of sub-areas of a slide; generating background intensity images; forming background-subtracted sub-area images by subtracting the minimum of a morphologically processed image and the original digital image from the original image; generating shrunk sub-area images by shrinking the background-subtracted sub-area images by a predefined scale factor; stitching the shrunk sub-area images into a composite image representing the entire slide; converting the composite image into a binary edge image by the application of an automatic thresholding technique; passing the binary edge image through a Hough transform to produce a Hough space transformed image; analyzing the Hough space transformed image to identify peaks representing a coverslip edge location; subjecting the Hough space transformed image to an inverse Hough transform to convert said peaks back to the edges of the coverslip in the binary edge image; setting to zero all pixels of the composite image outside of the coverslip area; morphologically processing the composite binary edge image; identifying colonies in the composite binary edge image; eliminating unwanted colonies from the composite binary edge image based on their size or morphology; recording the location, size, or binary image masks of the remaining colonies on the slide; generating a map of automatic focusing values over the remaining colonies on the slide using a high power objective; acquiring a plurality of high power images over the remaining colonies on the slide using a high power objective and the map of automatic focusing values; performing metaphase detection; and assigning each detected metaphase to a colony.