Patent Document ID: 8865467
Application ID: 13080728
Patent Status: 1

Claim One:
1. A lentiviral vector particle wherein the vector particle comprises a nucleic acid sequence comprising a sequence encoding: a. a first gene; b. a second gene; c. a third gene; d. fourth gene, wherein the four genes are Oct4, Sox2, Klf4 and cMyc, or Oct4, Sox2, Lin28, and Nanog; e. a first ‘self-cleaving’ 2A peptide; f. second ‘self-cleaving’ 2A peptide; and g. an internal ribosome entry site (IRES); wherein the first 2A ‘self-cleaving’ peptide is positioned between at least the first and second genes and the second ‘self-cleaving’ 2A peptide is positioned between the third and fourth genes, wherein the first 2A ‘self-cleaving’ peptide and second ‘self-cleaving’ 2A peptide are different, wherein the nucleic acid sequence is operably linked to a promoter, wherein the IRES is between the second and third genes, and wherein the sequences encoding the first, second, third and fourth genes, first and second ‘self-cleaving’ 2A peptides, and the IRES are transcribed from the promoter as a multi-cistronic RNA.