Patent Document ID: 10151697
Application ID: 15303461
Patent Status: 1

Claim One:
1. A two-color fluorescence localized super-resolution biological microscopy method, comprising the following steps: performing two-color fluorescence labeling on a biological sample by using Alexa647 and Alexa750 fluorescent molecules or Cy5 and Cy7 fluorescent molecules, and soaking the biological sample in an imaging buffer solution comprising TCEP (tri(2-carboxyethyl) phosphine and/or Thiol; illuminating the biological sample by laser to generate a blinking fluorescent signal of a first channel corresponding to the Alexa647 or Cy5 fluorescent molecules as well as a blinking fluorescent signal of a second channel corresponding to the Alexa750 or Cy7 fluorescent molecules respectively; constructing a super-resolution image of a first biological structure and a super-resolution image of a second biological structure respectively according to the blinking fluorescent signal of the first channel and the blinking fluorescent signal of the second channel; and aligning the super-resolution image of the first biological structure and the super-resolution image of the second biological structure to construct a super-resolution image of a third biological structure; wherein the step of illuminating the biological sample comprises: 1) illuminating the biological sample by an activation laser, and thereby activating a fluorescent signal, and 2) illuminating the biological sample by an excitation laser, and thereby deactivating the fluorescent signal and generating the blinking fluorescent signals; and wherein the TCEP or Thiol and the fluorescent molecules form an adduct under illumination of the excitation laser, the adduct does not fluoresce under illumination of the excitation laser, and wherein the adduct is redecomposed into the TCEP or Thiol and the fluorescent molecules fluoresce under illumination of the activation laser.