Patent Document ID: 9535001
Application ID: 14340125
Patent Status: 1

Claim One:
1. A cell counting method for counting cultured cells, comprising: acquiring an image of the cultured cells; separating an image of cell clusters and an image of individual cells from the image of the cultured cells; conducting circular approximation of the individual cells based on the image of the individual cells; calculating a number of the individual cells and an average area of the individual cells; calculating a volume of the cell cluster based on a projected area of the cell cluster that is assumed to be a sphere; calculating a volume of the individual cells based on the average area of the individual cells; and dividing the volume of the cell cluster by the volume of the individual cell, wherein the volume of the cell cluster is further divided by a correction coefficient selected from: (a) correction coefficient=1.1003×[the projected area of the cell cluster (×10 −5 cm 2 )]−0.1885 when the projected area of the cell cluster is greater than or equal to 1.0802×10 −5 cm 2 , or (b) correction coefficient=1 when the projected area of the cell cluster is less than 1.0802×10 −5 cm 2 .