Patent Document ID: 10002425
Application ID: 15369897
Patent Status: 1

Claim One:
1. A method of segmenting single neuron images with high-dynamic-range thresholds, comprising: preparing a biological tissue sample containing neurons and performing single three-dimensional imaging to said biological tissue sample containing neurons to obtain a single three-dimensional raw neuroimage; filtering out voxels in said three-dimensional raw neuroimage whose signal intensities are below a first signal intensity threshold, a second signal intensity threshold,. .. , a n-th signal intensity threshold respectively by a filtering module to obtain a first filtered image, a second filtered image,. .. , a n-th filtered image; tracing said first filtered image, said second filtered image,. .. , said n-th filtered image for their skeleton by a tracing module respectively to obtain a first traced image, a second traced image,. .. , a n-th traced image; calculating structural importance scores of every voxel in said first traced image, said second traced image,. .. , said n-th traced image by a score calculating module respectively by utilizing an equation to obtain a first structural importance score, a second structural importance score,. .. , a n-th structural importance score of every voxel; summing up said first structural importance score, said second structural importance score,. .. , and said n-th structural importance score of every voxel by a summing module to obtain a summed structural importance score of every voxel; determining a structural importance score threshold; and filtering out voxels in said three-dimensional raw neuroimage whose summed structural importance scores are below said structural importance score threshold by said filtering module to obtain a single neuron three-dimensional image, wherein n is a positive integer which is larger than 3.