Patent Document ID: 9150927
Application ID: 13501542
Patent Status: 1

Claim One:
1. A method for detecting or quantifying an insulin receptor isoform A (IR-A) nucleic acid in a biological sample or a sample of nucleic acids prepared from a biological sample, comprising the steps of: (i) contacting a biological sample or nucleic acids prepared from a biological sample with a primer set under conditions suitable for polymerase-based amplification; (ii) amplifying the IR-A nucleic acid by performing polymerase-based amplification; and (iii) detecting or quantifying the amplified IR-A nucleic acid using a kit; wherein the kit is selected from: (a) a kit comprising a 20 nucleotide IR-A forward synthetic nucleic acid of a nucleotide sequence as set forth in SEQ ID NO: 3, a 20 nucleotide IR-A reverse synthetic nucleic acid of a nucleotide sequence as set forth in SEQ ID NO: 21, and a 14 nucleotide synthetic nucleic acid IR-A probe of a nucleotide sequence set forth in SEQ ID NO: 7, wherein the probe comprises a detectable label; or (b) a kit comprising a 22 nucleotide IR-A forward synthetic nucleic acid of a nucleotide sequence as set forth in SEQ ID NO: 5, an 18 nucleotide IR-A reverse synthetic nucleic acid of a nucleotide sequence as set forth in SEQ ID NO: 22, and a 17 nucleotide synthetic nucleic acid IR-A probe of a nucleotide sequence as set forth in SEQ ID NO: 8 wherein the probe comprises a detectable label.