Patent Document ID: 5521298
Application ID: 08142232
Patent Status: 1

Claim One:
1. A labeled nucleotide probe formed according to the chemical synthesis having the following steps: a) providing a first target recognition moiety comprising a nucleotide sequence of at least about 15 nucleotide bases, said sequence containing a 5' end with a free 5' group and a 3' end with a free 3' group; b) altering said first target recognition moiety to contain a reactive functionality at said free 5' group of said 5' end, or said free 3' group of said 3' end, or said free 5' group of said 5' end and said free 3' group of said 3' end, wherein said reactive functionality is selected from the group consisting of carboxyl, amino, thiol, and sulphydryl; c) providing a second signal generating moiety comprising a nucleic acid sequence having at least one terminus and a length of from about 50 to about 200 bases, and further having at least one detectable label bound thereon wherein said terminus contains a functionality that is capable of reacting with said reactive functionality provided on said first target recognition moiety, said functionality selected from the group consisting of carboxyl, amino, thiol, and sulphydryl; d) chemically reacting said reactive functionality on said first target recognition moiety with said functionality on said second signal generating moiety to covalently join said first target recognition moiety and said second signal generating moiety thereby producing said labeled nucleotide probe.