Patent Document

LATIN NAME OF THE GENUS AND SPECIES OF THE PLANT CLAIMED 
       [0001]    
       Actinidia deliciosa  
     
       VARIETY DENOMINATION 
       [0002]    ‘Tsechelidis’ 
       BACKGROUND OF THE INVENTION 
       [0003]    Kiwi plants have been cultivated outside their native China for over one hundred years. Known varieties or cultivars include Hayward, Meteor, Hort16A, Abbott, Tomua, Jade Moon, Bruno, Monty, Matua and Kuimi. Hayward is the most popular variety worldwide. Kiwi plants are now commercially grown in New Zealand, Italy, Chile, France, Greece, Japan, China and the United States. 
         [0004]    Kiwi plants are commercially grown for their oblong or oval fruit, having brown skin covered in short hairs. The flesh, firm until fully ripe, is glistening, bright green or sometimes yellow, brownish or off-white, except for the white, succulent center from which radiate many fine, pale lines. Between these lines are scattered minute dark-purple or nearly black seeds, unnoticeable in eating. 
         [0005]    Kiwi plants may be propagated by seed, grafting or cutting. 
       SUMMARY OF THE INVENTION 
       [0006]    The present invention relates to a new and distinctive kiwi variety characterized by very large, oblong fruit covered with downy hairs, and very broad ovate leaves having acuminate apexes, among other features. The size and uniformity of the fruit provide significantly higher yield than other known varieties of kiwi. The new variety designated ‘Tsechelidis’ was derived from the ‘Hayward’ variety in Episkopi, Imathia, Greece and has been asexually reproduced by cutting, among other methods. 
         [0007]    ‘Tsechelidis’ is further distinguished by the nutritional characteristics of the fruit as well as the qualitative characteristics. Furthermore, a molecular genetic analysis distinguishes ‘Tsechelidis’ from ‘Hayward’ as indicated by several polymorphisms in known alleles. 
     
    
     
       BRIEF DESCRIPTION OF THE PHOTOGRAPHS 
         [0008]    The accompanying color photographs of ‘Tsechelidis’ show the new variety as well as comparisons of the ‘Tsechelidis’ variety to the ‘Hayward’ variety. 
           [0009]      FIG. 1  shows a typical leaf of ‘Tsechelidis’. 
           [0010]      FIG. 2  shows typical flowers of ‘Tsechelidis’. 
           [0011]      FIG. 3  shows a typical flower of ‘Tsechelidis’ as compared to a typical flower of ‘Hayward’. 
           [0012]      FIG. 4  shows typical fruit of ‘Tsechelidis’ on the vine. 
           [0013]      FIG. 5  shows typical fruit of ‘Tsechelidis’ on the vine as compared to typical fruit of ‘Hayward’ on the vine. 
           [0014]      FIG. 6  shows typical fruit of ‘Tsechelidis’ with stems attached. 
           [0015]      FIG. 7  shows a cross-section and a stylar end view of a typical fruit of ‘Tsechelidis’ 
           [0016]      FIG. 8  shows a cross-section and a side view of a typical fruit of ‘Tsechelidis’. 
           [0017]      FIG. 9  shows typical fruit of ‘Tsechelidis’ as compared to typical fruit of ‘Hayward’. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0018]    The present invention is a new and distinct variety of  Actinidia deliciosa  plants having the several characteristics that distinguish the variety from other kiwi plants, particularly the ‘Hayward’ variety. 
         [0019]    The new variety ‘Tsechelidis’ was discovered in Episkopi, Imathia, Greece, when the inventor planted seeds from selected Hayward plants with the purpose of obtaining a group of male plants. From this original group of twenty plants, a single plant was identified as female. During its first harvest in 1994, the female plant bore unusually large fruit as compared to plants of the ‘Hayward’ variety. The female plant was monitored for the next two harvests and consistently bore the unusually large fruit, as well as other features which distinguished it from ‘Hayward’. The female plant was then used to propagate the ‘Tsechelidis’ variety by asexual reproduction beginning in 1996. 
         [0020]    The ‘Tsechelidis’ variety has been continuously asexually reproduced by cuttings from 1996 through 2007. The distinguishing characteristics of the variety continue to run true in the propagated plants, as shown by about 1000 plants covering ½ hectare. 
         [0000]    Furthermore, no changes have appeared in the new variety when grafted on ‘Hayward’. In addition to cuttings, the new variety can be asexually reproduced by grafting to rootstocks of  A. deliciosa.    
         [0021]    Male pollenizers suitable for ‘Tsechelidis’ may include, but are not limited to, ‘Matua’. At this time the ‘Tsechelidis’ variety is being tested for self-pollenization by the University of Volvos, Greece. According to preliminary results, ‘Tsechelidis’ is characterized by up to 75% self-pollenization as compared to 2% for ‘Hayward’. 
         [0022]    ‘Tsechelidis’ should be cultivated in areas that are not affected by spring frost. Temperatures of less than −2° C. will damage the tender shoots of the plant and suspending growth or reduce the setting process. Also, frost in early autumn to late October may damage fruit situated on the vine. The presence of strong wind, particularly in May, may result in considerable decrease of flowers borne by the plant. 
         [0000]    Distinguishing Characteristics from Known Varieties 
         [0023]    The following is a comparison of the fruit production an orchard of ‘Tsechelidis’ as compared with cultivated ‘Hayward’ situated in the area of Episkopi-Anthemion in the prefecture of Imathia, Greece. At the time of this study (2006) the ‘Tsechelidis’ orchard was five years old. 
         [0024]    The listed observations, measurements and assessments were made in the following categories: 
         [0025]    1. Plant and fruit characteristics 
         [0026]    2. Quantitative production data (yield, number of fruits/plants and average fruit weight). 
         [0027]    3. Level of nutritional elements in leaves and fruit. 
         [0028]    4. Qualitative fruit characteristics (resistance to pressure, pH, soluble solids (°Brix), vitamin C, acids, sugars, Thiault index and dry matter). 
       1. Plant and Fruit Characteristics: 
       [0029]    The chart below describes the physical differences between ‘Tsechelidis’ and the known ‘Hayward’ variety. The Horticultural terminology applied below is used in accordance with revised UPOV guidelines for kiwi (Test Guidelines—TG/98/6— Actinidia  2001-04-04, available at http://www.upov.int/en/publications/tg-rom/tg098/tg — 98 — 6.pdf). 
         [0000]    
       
         
               
               
               
             
           
               
                   
               
               
                 UPOV Characteristics 
                   
                   
               
               
                 for comparison of varieties 
                 ‘HAYWARD’ 
                 ‘TSECHELIDIS’ 
               
               
                   
               
             
             
               
                 Plant: vigor 
                 Medium 
                 Strong 
               
               
                 Leaf blade: shape 
                 Broad ovate 
                 Very broad ovate 
               
               
                 Leaf blade: shape of apex 
                 Acute 
                 Acuminate 
               
               
                 Leaf blade: green color 
                 Medium 
                 Dark 
               
               
                 of upper side 
               
               
                 Petal: curvature of apex 
                 Strongly 
                 Very strongly expressed 
               
               
                   
                 expressed 
               
               
                 Fruit: size 
                 Large 
                 Very large 
               
               
                 Fruit: general shape 
                 Ellipsoid 
                 Oblong 
               
               
                 Fruit: general shape 
                 Flat 
                 Between slightly depressed 
               
               
                 of stylar end 
                   
                 and flat 
               
               
                 Fruit: shape of shoulder 
                 Rounded 
                 Squared 
               
               
                 at stalk end 
               
               
                 Fruit: type of hairiness 
                 Hirsute 
                 Downy 
               
               
                 Time of beginning 
                 Late 
                 Medium 
               
               
                 of flowering 
               
               
                   
               
             
          
         
       
     
         [0030]    The shoots of ‘Tsechelidis’ grow more vigorously than ‘Hayward’, though there is no difference with respect to resilience against pest and diseases. The fruits of ‘Tsechelidis’ are more oblong than ‘Hayward’ (the ratio of fruit length/width is 1.41 and 1.24 respectively). Furthermore, the fruits of ‘Tsechelidis’ are larger and more uniform in size. ‘Tsechelidis’ do no require thinning absent defective fruit, unlike ‘Hayward’. Additionally, due to the larger fruit size, any decrease that may be caused by low temperatures during the flowering season or poor pollination of the fruits will not affect the marketability of the fruits, in contrast with ‘Hayward’. 
       2. Quantitative Production Data: 
       [0031]      
         [0000]                                                                      Significance       Parameter   ‘TSECHELIDIS’   ‘HAYWARD’   level (P)                                Total number of   250   279   P &gt; 0.05       fruits/plant       Number of   249   222   P &gt; 0.05       marketable fruits       Rate of   99.6   79.6   —       marketable fruits       (%)       Total yield (kg/   41.9   29.9   P &gt; 0.05       plant)       Yield of   41.6   25.1   0.001 &lt; P &lt; 0.01       marketable       fruits (kg/plant)       Yield of   99.3   83.9   —       marketable fruits       (%)       Average weight   167.0   114.5   P &lt; 0.001       of marketable       fruits (g)       Average weight   237.0   88.8   P &lt; 0.001       of non-marketable       fruits (g)               P &gt; 0.05 signifies a statistically insignificant difference.            
‘Tsechelidis’ has a greater density of buds in each stem than ‘Hayward’, whereby each stem, which has 13 buds, produces about 10 kg of fruit. Therefore, each ‘Tsechelidis’ tree, having about 15-18 stems, yields about 140-150 kg of fruit. The high yield, and large size and uniformity of the fruit of ‘Tsechelidis’, as compared to the ‘Hayward’, are significant advantages, particularly with regard to reducing production costs. This data was taken during a harvest affected by adverse weather during the growing season in Imathia, Greece.
 
       3. Level of Nutritional Elements 
       [0032]    The following is table that shows the statistically significant differences in nutritional elements between ‘Tsechelidis’ and ‘Hayward’. 
         [0000]    
       
         
               
               
               
               
             
           
               
                   
                   
               
               
                   
                 Parameter 
                 ‘TSECHELIDIS’ 
                 ‘HAYWARD’ 
               
               
                   
                   
               
             
             
               
                   
                 Leaves: nitrogen level 
                 1.95% 
                 2.53% 
               
               
                   
                 Fruit skin: phosphorus 
                 0.13% 
                 0.08% 
               
               
                   
                 Fruit skin: potassium 
                 2.35% 
                 1.95% 
               
               
                   
                 Fruit skin: magnesium 
                 0.08% 
                 0.06% 
               
               
                   
                 Fruit skin: manganese 
                 12.6 ppm 
                  8.0 ppm 
               
               
                   
                 Fruit flesh: nitrogen 
                 0.76% 
                 0.95% 
               
               
                   
                 Fruit flesh: phosphorus 
                 0.13% 
                 0.16% 
               
               
                   
                 Fruit flesh: manganese 
                 10.3 ppm 
                  6.2 ppm 
               
               
                   
                 Fruit flesh: copper 
                 6.79 ppm 
                 10.51 ppm 
               
               
                   
                 Fruit flesh: proportion 
                 2.30 
                 2.71 
               
               
                   
                 of N/Ca 
               
               
                   
                   
               
             
          
         
       
     
       4. Qualitative Fruit Characteristics 
       [0033]    The following tables show specific qualitative fruit characteristics between ‘Tsechelidis’ and ‘Hayward’. The first table lists measurements of fruit immediately after harvest. The second table lists measurements taken of fruit held in refrigerated storage for two months after harvest. 
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                   
               
               
                 Measurements Taken During Harvest 
               
             
          
           
               
                   
                   
                   
                 Significance 
               
               
                 Parameter 
                 ‘TSECHELIDIS’ 
                 ‘HAYWARD’ 
                 level (P) 
               
               
                   
               
             
          
           
               
                 Resistance to 
                 23.0 
                 27.0 
                 0.001 &lt; P &lt; 0.01 
               
               
                 pressure (lb/in 2 ) 
               
               
                 Flesh pH 
                 3.34 
                 3.25 
                  0.01 &lt; P &lt; 0.05 
               
               
                 Soluble solids 
                 7.30 
                 6.70 
                 P &gt; 0.05 
               
               
                 (°Brix) (%) 
               
               
                 Vitamin C 
                 79.2 
                 37.8 
                 P &lt; 0.001 
               
               
                 (mg/100 g 
               
               
                 fresh weight) 
               
               
                 Malic acid (g/l) 
                 4.5 
                 4.0 
                 P &gt; 0.05 
               
               
                 Sugars (g/l) 
                 62.8 
                 57.2 
                 0.01 &lt; P &lt; 0.05 
               
               
                 Thiault index 
                 107.7 
                 97.6 
                 0.01 &lt; P &lt; 0.05 
               
               
                 Dry matter (%) 
                 15.30 
                 15.82 
                 P &gt; 0.05 
               
               
                   
               
             
          
         
       
     
         [0000]                                                                Measurements Taken Two (2) Months After Harvest                        Significance       Parameter   ‘TSECHELIDIS’   ‘HAYWARD’   level (P)                    Resistance to   10.   10.9   P &gt; 0.05       pressure (lb/in 2 )       Flesh pH   3.32   3.41   P &lt; 0.001       Soluble solids   13.6   13.0   P &gt; 0.05       (°Brix) (%)       Vitamin C (mg/100   80.2   38.3   P &lt; 0.001       g fresh weight)       Malic acid (g/l)   4.8   4.5   P &gt; 0.05       Sugars (g/l)   84.4   80.0   P &gt; 0.05       Thiault index   132.4   125.0   P &gt; 0.05                    
Based on the above information, the following distinctions may be drawn between ‘Tsechelidis’ and ‘Hayward’. The fruit of ‘Tsechelidis’ ripen 7-10 days earlier than ‘Hayward’, which is shown by the fruit&#39;s lower resistance to pressure and their slight superiority in soluble solids (°Brix) during harvest. Despite the earlier ripening, the resistance to pressure of ‘Tsechelidis’ after two months of refrigerated storage was the same as ‘Hayward’.
 
       Molecular Genetic Analysis 
       [0034]    A molecular genetic analysis was conducted by Dr. Athanasios Mavromatis, Professor of Genetics &amp; Plant Breeding (University of Thessaly, School of Agricultural Services) comparing ‘Tsechelidis’ with ‘Hayward’ based on known microsatellite DNA markers using PCR. The method is recognized as an accurate and repeatable molecular analysis for Actinidia. Huang, W. G., Cipriani, G., Morgante, M., Testolin, R. (1998) Microsatellite DNA in  Actinidia chinensis:  isolation, characterization, and homology in related species.  Theor. Appl. Genet.  97 (8): 1269-1278. 
         [0035]    The DNA analysis was performed as follows: 
         [0036]    Repeatable samples of four genotypes were used (commercial clones of ‘Hayward’ (one female, one male) and ‘Tsechelidis’ (one female, one male)). Leaf samples of 0.3 g per genotype was used for DNA extraction process according to a modified cetyltrimethylammonium bromide (CTAB) method. The extract DNA was quantified on agarose gel by comparison with report samples (DNA marker). The DNA quality and quantity was tested to ensure accuracy of the molecular genetic analysis. 
         [0037]    Thirteen known primer pairs were used for amplifying dinucleotide tandems AG/CT and AC/GT. The primer pairs used were: UDK 96-022, UDK 97-402, UDK 99-152, UDK 96-053, UDK 97-411, UDK 96-030, UDK 96-001, UDK 96-037, UDK 96-034, UDK 99-168, UDK 96-092, UDK 97-406 and UDK 97-407. The primers were developed in a bilateral European Union International Cooperation with Developing Countries (INCO-DC) project performed by University of Udine, Italy; Chinese Agricultural University, Beijing China; INRA, France; and University of Thessaly, Greece. 
         [0038]    The PCR products were separated in 6% polyacrylamide gels 1.5 mm thick. Band visualization was made with silver nitrate. The results of the study indicated that at least seven out of 13 primer pairs were polymorphic. Therefore, the study provides grounds for distinguishing between genetic material from ‘Tsechelidis’ as compared to ‘Hayward’. 
         [0039]    The polymorphic primer pairs are described in the table below according to the presence and absence of alleles of the same molecular weight. 
         [0000]    
       
         
               
               
               
               
             
           
               
                   
                   
               
               
                   
                 DNA primer/alleles 
                 ‘TSECHELIDIS’ 
                 ‘HAYWARD’ 
               
               
                   
                   
               
             
             
               
                   
                 99-152 
                   
                   
               
               
                   
                 97-411 
                 + 
                 − 
               
               
                   
                 96-030 
               
               
                   
                 96-037α 
                 − 
                 + 
               
               
                   
                 96-037β 
                 + 
                 − 
               
               
                   
                 96-034 
                 + 
                 − 
               
               
                   
                 96-092 
                 + 
                 − 
               
               
                   
                 97-406 
                 − 
                 + 
               
               
                   
                   
               
             
          
         
       
     
         [0040]    The genetic difference confirmed through diverse binding patterns indicate that the ‘Tsechelidis’ variety is genetically distinct from ‘Hayward’.

Technology Category: 1