Patent Document

RELATED APPLICATIONS 
       [0001]    This application is a continuation-in-part of U.S. application Ser. No. 11/326,185 entitled BIOREACTOR SYSTEMS, filed Jan. 4, 2006, which is related to and claims priority of U.S. Provisional Application Ser. No. 60/641,523 entitled BIOREACTOR SYSTEMS, filed Jan. 4, 2005, the contents of each of which are incorporated herein by reference as if set forth in their entireties. 
     
    
     FIELD OF THE INVENTION 
       [0002]    This invention relates to providing improved bioreactor systems and bioreaction methods. More particularly, this invention relates to providing improved bioreactors providing automatic serial batch production of live microbes. More particularly, this invention relates to providing improved bioreactors which use preloaded disposable bioreactor chambers. 
       BACKGROUND 
       [0003]    Typically, bioreactors use a metal chamber to incubate one batch of microbes, and then such chamber must be sterilized between, each use. Disposable bioreactor chambers, such as bioreactor bags, must be manually monitored during use. Both these types of bioreactors are presently loaded with a starter culture of microbes at the beginning of the incubation process by the end-user, which increases the possibility of contamination of the bioreactor by pathogens, especially in agricultural settings. 
         [0004]    Therefore, a need exists for a bioreactor system adapted to provide more-continuous batch incubation of microbes which system would provide for greatly decreased chances of such contamination. 
       OBJECTS AND FEATURES OF THE INVENTION 
       [0005]    A primary object and feature of the present invention is to provide bioreactor systems. A further primary object and feature of the present invention is to provide bioreactor systems adapted to provide continuous batch incubation of microbes. 
         [0006]    It is a further object and feature of the present invention to provide such a system having sterile preloaded bioreactor chambers. It is a further object and feature of the present invention to provide such a system comprising a self-contained housing for the sterile preloaded bioreactor chambers. It is a further object and feature of the present invention to provide such a system capable of running automatically for several days or weeks at a time. 
         [0007]    It is a further object and feature of the present invention to provide methods of automating microbial treatment of specified biota growing in specified ground. 
         [0008]    It is a further object and feature of the present invention to provide such a system comprising methods of manufacture, methods of use, methods of sales, and methods of farming. 
         [0009]    A further primary object and feature of the present invention is to provide such a system that is efficient, inexpensive, and handy. Other objects and features of this invention will become apparent with reference to the following descriptions. 
       SUMMARY OF THE INVENTION 
       [0010]    In accordance with an embodiment hereof, one aspect of this invention provides a bioreactor system, comprising: at least one bioreactor container adapted to contain at least one bioreaction; at least one microbe adapted to provide at least one living microbe; wherein such at least one microbe is in an inert state; and wherein such at least one microbe is stored within such at least one bioreactor container. The inert microbe may initially be in a substantially wet state or it may be in a substantially dry state. 
         [0011]    Moreover, it provides such a bioreactor system, further comprising at least one first water-soluble container adapted to contain such at least one microbe in at least one water-soluble container. Additionally, it provides such a bioreactor system, further comprising at least one nutrient adapted to provide at least one nutrient adapted to support the life of such at least one at least one microbe, wherein such at least one at least one nutrient is in an inert, dry state; and wherein such at least one nutrient is stored within such at least one bioreactor container. Also, it provides such a bioreactor system, further comprising at least one second water-soluble container adapted to contain such at least one nutrient in at least one water-soluble container. In addition, it provides such a bioreactor system, further comprising at least one enzyme adapted to provide at least one enzyme adapted to support the life of such at least one at least one microbe, wherein such at least one at least one enzyme is in an inert, dry state; and wherein such at least one enzyme is stored within such at least one bioreactor container. 
         [0012]    And, it provides such a bioreactor system, further comprising at least one third water-soluble container adapted to contain such at least one enzyme in at least one water-soluble container. Further, it provides such a bioreactor system, further comprising: at least one nutrient adapted to provide at least one nutrient adapted to support the life of such at least one at least one microbe, wherein such at least one at least one nutrient is in an inert, dry state; and wherein such at least one nutrient is stored within such at least one bioreactor container; at least one enzyme adapted to provide at least one enzyme adapted to support the life of such at least one at least one microbe, wherein such at least one at least one enzyme is in an inert, dry state; and wherein such at least one enzyme is stored within such at least one bioreactor container. 
         [0013]    Even further, it provides such a bioreactor system, further comprising at least one fourth water-soluble container adapted to contain such at least one microbe, such at least one nutrient, and such at least one enzyme in at least one water-soluble container. Moreover, it provides such a bioreactor system, wherein such at least one microbe comprises at least one bacteria. Additionally, it provides such a bioreactor system, wherein such at least one bioreactor container comprises at least one flexible container. Also, it provides such a bioreactor system, wherein at least one interior of such at least one bioreactor container, containing such at least one microbe, is sterile of pathogens. 
         [0014]    In accordance with another embodiment hereof, this invention provides a bioreactor system, comprising: at least one flexible container adapted to flexibly contain at least one fluid containing at least one cell culture; at least one fluid input adapted to input fluid into such at least one flexible container; at least one fluid output adapted to output fluid from such at least one flexible container; at least one gas input adapted to input at least one gas into such at least one flexible container; wherein such at least one gas input is adapted to create bubbles in such at least one fluid; at least one gas output adapted to output at least one gas from such at least one flexible container. 
         [0015]    In addition, it provides such a bioreactor system, further comprising at least one fluid input manifold adapted to provide at least one fluid to such at least one fluid input. And, it provides such a bioreactor system, wherein such at least one fluid input manifold connects to a plurality of such at least one fluid inputs. Further, it provides such a bioreactor system, wherein such at least one fluid input comprises at least one connector adapted to connect to and disconnect from such at least one fluid input manifold without tools. 
         [0016]    Even further, it provides such a bioreactor system, further comprising at least one fluid source adapted to provide at least one source of at least one fluid. Moreover, it provides such a bioreactor system, further comprising such at least one fluid, and wherein such at least one fluid comprises sterilized water. Additionally, it provides such a bioreactor system, wherein such sterilized water is sterilized with ultraviolet radiation treatment. Also, it provides such a bioreactor system, wherein such sterilized water is sterilized by reverse osmosis treatment. In addition, it provides such a bioreactor system, wherein such at least one fluid comprises temperature-controlled water. 
         [0017]    And, it provides such a bioreactor system, further comprising at least one fluid output manifold adapted to receive fluid from such at least one fluid output. Further, it provides such a bioreactor system, wherein such at least one fluid output manifold connects to a plurality of such at least one fluid outputs. Even further, it provides such a bioreactor system, wherein such at least one fluid output comprises at least one connector adapted to quickly connect to and disconnect from such at least one fluid output manifold without tools. Moreover, it provides such a bioreactor system, further comprising at least one gas input manifold adapted to provide gas to such at least one gas input. Additionally, it provides such a bioreactor system, wherein such at least one gas input manifold connects to a plurality of such at least one gas inputs. 
         [0018]    Also, it provides such a bioreactor system, wherein such at least one gas input comprises at least one connector adapted to quickly connect to and disconnect from such at least one gas input manifold without tools. In addition, it provides such a bioreactor system, further comprising at least one gas source adapted to provide at least one source of gas to such at least one gas input manifold. And, it provides such a bioreactor system, wherein such at least one gas source comprises at least one gas pump. Further, it provides such a bioreactor system, further comprising atmospheric air, wherein such atmospheric air comprises such at least one gas source. Even further, it provides such a bioreactor system, wherein such at least one gas source comprises at least one oxygen generator. Moreover, it provides such a bioreactor system, wherein such at least one gas source comprises at least one compressed gas container. 
         [0019]    Additionally, it provides such a bioreactor system, further comprising at least one gas output manifold adapted to receive gas from such at least one gas output. Also, it provides such a bioreactor system, wherein such at least one gas output manifold connects to a plurality of such at least one gas outputs. In addition, it provides such a bioreactor system, wherein such at least one gas output comprises quick-at least one connector adapted to quickly connecting to and disconnecting from such at least one gas output manifold. And, it provides such a bioreactor system, wherein such at least one flexible container has a volume of about 30 gallons. Further, it provides such a bioreactor system, wherein such at least one flexible container is opaque. 
         [0020]    Even further, it provides such a bioreactor system, further comprising at least one rigid stackable container adapted to provide at least one rigid stackable container adapted to hold such at least one flexible container. Moreover, it provides such a bioreactor system, wherein such at least one rigid stackable container comprises at least one rigid stackable box. Additionally, it provides such a bioreactor system, wherein such at least one rigid stackable box comprises cardboard but other rigid materials may be used in other exemplary embodiments. 
         [0021]    Also, it provides such a bioreactor system, wherein such at least one fluid input, such at least one fluid output, such at least one gas input, and such at least one gas output are each heat-sealed to such at least one flexible container. In addition, it provides such a bioreactor system, wherein such at least one fluid input comprises at least one one-way valve. And, it provides such a bioreactor system, wherein such at least one fluid output comprises at least one one-way valve. Further, it provides such a bioreactor system, wherein such at least one gas input comprises at least one one-way valve. Even further, it provides such a bioreactor system, wherein such at least one gas output comprises at least one one-way valve. 
         [0022]    Moreover, it provides such a bioreactor system, wherein such at least one fluid input comprises at least one filter. Additionally, it provides such a bioreactor system, wherein such at least one gas input comprises at least one filter. Also, it provides such a bioreactor system, wherein such at least one gas output comprises at least one filter. In addition, it provides such a bioreactor system, wherein such at least one gas input comprises at least one aerator. 
         [0023]    And, it provides such a bioreactor system, further comprising at least one controller adapted to control the contents of such at least one flexible container. Further, it provides such a bioreactor system, wherein such at least one controller controls the flow of fluid from such at least one fluid source to such at least one fluid input manifold. Even further, it provides such a bioreactor system, wherein such at least one controller controls the flow of fluid from such at least one fluid input manifold to such at least one fluid input. Moreover, it provides such a bioreactor system, wherein such at least one controller controls the flow of fluid from such at least one fluid output to such at least one fluid output manifold. Additionally, it provides such a bioreactor system, wherein such at least one controller controls the flow of fluid from such at least one fluid output manifold to at least one fluid destination. In other words, the controller controls which flexible container of the at least one flexible container has fluid delivered thereto and therefrom thereby determining which flexible container is utilized. Also, it provides such a bioreactor system, wherein such at least one controller controls the flow of gas from such at least one gas source to such at least one gas input manifold. 
         [0024]    In addition, it provides such a bioreactor system, wherein such at least one controller controls the flow of gas from such at least one gas input manifold to such at least one gas input. And, it provides such a bioreactor system, wherein such at least one controller controls the flow of gas from such at least one gas output to such at least one gas output manifold. Further, it provides such a bioreactor system, wherein such at least one controller controls the flow of gas from such at least one gas output manifold to at least one gas destination. Even further, it provides such a bioreactor system, wherein such at least one controller comprises at least one programmable irrigation timer. 
         [0025]    Moreover, it provides such a bioreactor system, further comprising at least one enclosure adapted to enclose at least such at least one flexible container. Additionally, it provides such a bioreactor system, wherein such at least one enclosure maintains such at least one flexible container at least one selected temperature. Also, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one heat pump. In addition, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one thermostat. And, it provides such a bioreactor system, wherein such at least one enclosure is thermally insulated. Further, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one fluid input manifold adapted to provide fluid to such at least one fluid input. Even further, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one fluid output manifold adapted to receive fluid from such at least one fluid output. 
         [0026]    Moreover, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one gas input manifold adapted to provide gas to such at least one gas input. Additionally, it provides such a bioreactor system, further comprising at least one gas source adapted to provide at least one source of gas to such at least one gas input manifold. Also, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one gas output manifold adapted to receive gas from such at least one gas output. In addition, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one controller adapted to control the contents of such at least one flexible container. 
         [0027]    And, it provides such a bioreactor system, wherein such at least one enclosure encloses about eight of such at least one flexible containers. Further, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one transport adapted to transporting such at least one enclosure. Even further, it provides such a bioreactor system, wherein such at least one transport comprises at least one wheel. Moreover, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one access door. 
         [0028]    Additionally, it provides such a bioreactor system, further comprising at least one irrigation system adapted to irrigate at least one crop. Also, it provides such a bioreactor system, further comprising at least one irrigation system input adapted to input such fluid output from such at least one flexible container into such at least one irrigation system. In addition, it provides such a bioreactor system, wherein such at least one irrigation system input comprises at least one fluid pump. And, it provides such a bioreactor system, wherein such at least one irrigation system input comprises at least one venturi suction unit. Further, it provides such a bioreactor system, wherein such at least one irrigation system input comprises at least one storage tank. Even further, it provides such a bioreactor system, wherein about 400 acres of crops are treated per 30 gallon flexible container. 
         [0029]    In accordance with another embodiment hereof, this invention provides a bioreactor system, relating to housing and maintaining at least one bioreaction in at least two bioreactor chambers, comprising: at least one enclosure adapted to enclose such at least two bioreactor chambers; wherein such at least one enclosure is thermally insulated; wherein such at least one enclosure comprises at least one temperature control adapted to control at least one temperature within such at least one enclosure; wherein such at least one enclosure comprises at least one fluid input manifold adapted to provide at least one fluid to each of such at least two bioreactor chambers; wherein such at least one enclosure comprises at least one fluid output manifold adapted to receive fluid from each of such at least two bioreactor chambers; wherein such at least one enclosure comprises at least one gas input manifold adapted to provide gas to each of such at least two bioreactor chambers; wherein such at least one enclosure comprises at least one gas output manifold adapted to receive gas from each of such at least two bioreactor chambers; wherein such at least one enclosure comprises at least one controller adapted to control such at least one temperature control, such at least one fluid input manifold, such at least one fluid output manifold, such at least one gas input manifold, and such at least one gas output manifold. 
         [0030]    Moreover, it provides such a bioreactor system, wherein such at least one controller comprises at least one programmable controller. Additionally, it provides such a bioreactor system, wherein such at least one temperature control comprises at least one heat pump. Also, it provides such a bioreactor system, wherein such at least one temperature control comprises at least one thermostat. In addition, it provides such a bioreactor system, further comprising at least one gas source adapted to provide at least one gas to such at least one gas input manifold. And, it provides such a bioreactor system, further comprising at least one fluid source adapted to provide at least one fluid to such at least one fluid input manifold. Further, it provides such a bioreactor system, wherein such at least one enclosure encloses about eight of such at least two bioreactor chambers. Even further, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one transport adapted to transporting such at least one enclosure. Moreover, it provides such a bioreactor system, wherein such at least one transport comprises at least one wheel. Additionally, it provides such a bioreactor system, wherein such at least one enclosure comprises at least one access door. 
         [0031]    In accordance with another embodiment hereof, this invention provides a bioreactor system, comprising: at least one flexible container adapted to flexibly contain at least one aqueous solution containing at least one cell culture; at least one fluid input adapted to input at least one fluid into such at least one flexible container; at least one fluid output adapted to output such at least one aqueous solution from such at least one flexible container; at least one gas input adapted to input at least one gas into such at least one flexible container; at least one gas output adapted to output at least one gas from such at least one flexible container; at least one enclosure adapted to enclose such at least one flexible container; wherein such at least one enclosure is thermally insulated; at least one temperature control adapted to control at least one temperature within such at least one enclosure; at least one fluid input manifold adapted to provide at least one fluid to such at least one fluid input; at least one fluid output manifold adapted to receive fluid from such at least one fluid output; at least one gas input manifold adapted to provide gas to such at least one gas input; at least one gas output manifold adapted to receive gas from such at least one gas output; and at least one controller adapted to control such at least one temperature control, such at least one fluid input manifold, such at least one fluid output manifold, such at least one gas input manifold, and such at least one gas output manifold. 
         [0032]    In accordance with another embodiment hereof, this invention provides a method for bioreaction in a bioreactor system, comprising the steps of: selecting at least one inert microbe; selecting at least one dry, inert nutrient adapted to support the life of such at least one inert microbe; placing such at least one inert microbe and such at least one dry, inert nutrient into at least one sterile bioreactor chamber; and storing such at least one sterile bioreactor chamber containing such at least one inert microbe and such at least one dry, inert nutrient. 
         [0033]    Also, it provides such a method further comprising the step of shipping such at least one sterile bioreactor chamber containing at least one inert microbe and such at least one dry, inert nutrient to at least one user. In addition, it provides such a bioreactor system, further comprising the steps of: adding water to such at least one sterile bioreactor chamber containing such at least one inert microbe and such at least one dry, inert nutrient; and permitting at least one bioreaction to occur. 
         [0034]    And, it provides such a bioreactor system, further comprising the step of placing such at least one dry, inert microbe into at least one water-soluble container prior to placing such at least one dry, inert microbe into such at least one sterile bioreactor chamber. Further, it provides such a bioreactor system, further comprising the step of placing such at least one dry, inert nutrient into at least one water-soluble container prior to placing such at least one dry, inert nutrient into such at least one sterile bioreactor chamber. Even further, it provides such a bioreactor system, further comprising the steps of: selecting at least one dry, inert enzyme adapted to support the life of such at least one dry, inert microbe; placing such at least one dry, inert enzyme into such at least one sterile bioreactor chamber. Moreover, it provides such a bioreactor system, further comprising the step of placing such at least one dry, inert enzyme into at least one water-soluble container prior to placing such at least one dry, inert enzyme into such at least one sterile bioreactor chamber. 
         [0035]    In accordance with another preferred embodiment hereof, this invention provides a bioreactor system, comprising the steps of: selecting at least one dry, inert microbe; selecting at least one dry, inert nutrient adapted to support the life of such at least one dry, inert microbe; selecting at least one previously sterilized bioreactor chamber; receiving such at least one bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient from at least one manufacturer; installing such at least one bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient in at least one bioreactor; adding fluid to such at least one bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient; waiting for at least one bioreaction in such at least one bioreactor chamber to generate a useful number of live, active microbes; and harvesting such useful number of live, active microbes. 
         [0036]    Additionally, it provides such a bioreactor system, further comprising the step of adding oxygen to such at least one bioreactor chamber, after such step of adding fluid to such at least one bioreactor chamber. Also, it provides such a bioreactor system, wherein such step of harvesting such useful number of live, active microbe comprises the step of adding such useful number of live, active microbes into at least one irrigation system. In addition, it provides such a bioreactor system, further comprising the steps of: uninstalling such at least one bioreactor chamber; and disposing of such at least one bioreactor chamber; after such step of harvesting such useful number of live, active microbes. 
         [0037]    In accordance with another preferred embodiment hereof, this invention provides a bioreactor system, comprising the steps of: analyzing at least one soil for at least one user; developing at least one bioremediation prescription for such at least one analyzed soil; loading at least one bioreactor chamber with dry, inert microbes according to such at least one bioremediation prescription; providing such at least one loaded bioreactor chamber to such at least one user. And, it provides such a bioreactor system, further comprising the step of maintaining at least one bioreactor on such at least one user&#39;s site. Further, it provides such a bioreactor system, further comprising the step of remotely monitoring such at least one bioreactor. Even further, it provides such a bioreactor system, further comprising the step of installing such at least one loaded bioreactor chamber in at least one bioreactor on such at least one user&#39;s site. 
         [0038]    Moreover, it provides such a bioreactor system, further comprising the step of installing such at least one loaded bioreactor chamber in at least one bioreactor on such at least one user&#39;s site according to at least one maintenance schedule. Additionally, it provides such a bioreactor system, further comprising the step of applying the bioreaction products of such at least one preloaded bioreactor chamber to such at least one analyzed soil. Also, it provides such a bioreactor system, further comprising the step of re-analyzing such at least one analyzed soil to determine the effects of applying such bioreaction products. 
         [0039]    In accordance with another embodiment hereof, this invention provides a bioreactor system, comprising the steps of: analyzing at least one soil for at least one user; analyzing at least one chemical treatment history of such at least one soil; developing at least one bioremediation prescription for such at least one analyzed soil; providing at least one on-site bioreactor wherein such at least one on-site bioreactor comprises at least one programmable controller; providing at least one loaded bioreactor chamber containing dry, inert microbes, according to such at least one bioremediation prescription, adapted to be used with such on-site bioreactor; programming such at least one on-site bioreactor to incubate such microbes in such at least one loaded bioreactor chamber; and programming such at least one on-site bioreactor to dispense such incubated microbes. 
         [0040]    In addition, it provides such a bioreactor system, wherein such step of developing at least one bioremediation prescription for such at least one analyzed soil is adapted to increase the functionality of crops grown in such at least one analyzed soil. And, it provides such a bioreactor system, wherein such step of developing at least one bioremediation prescription for such at least one analyzed soil is adapted to modify the growth of at least one cover crop grown in such at least one analyzed soil, wherein the modification of the growth of such at least one cover crop has at least one beneficial effect on the growth of at least one cash crop grown in such at least one analyzed soil after such at least one cover crop. 
         [0041]    Further, it provides such a bioreactor system, wherein such step of developing at least one bioremediation prescription for such at least one analyzed soil is adapted to benefit bodies of water adjacent such at least one analyzed soil. Even further, it provides such a bioreactor system, wherein such step of developing at least one bioremediation prescription for such at least one analyzed soil is adapted to decrease the concentration of toxins of crops grown in such at least one analyzed soil. 
         [0042]    In accordance with another embodiment hereof, this invention provides a bioreactor system, comprising: bioreactor container means for containing at least one bioreaction; microbe means for providing at least one living microbe; wherein such microbe means is in an inert, dry state; and wherein such microbe means is stored within such bioreactor container means. 
         [0043]    Moreover, it provides such a bioreactor system, further comprising first water-soluble container means for containing such microbe means in at least one water-soluble container. Additionally, it provides such a bioreactor system, further comprising nutrient means for providing at least one nutrient adapted to support the life of such at least one microbe means, wherein such at least one nutrient means is in an inert, dry state; and wherein such nutrient means is stored within such bioreactor container means. Also, it provides such a bioreactor system, further comprising second water-soluble container means for containing such nutrient means in at least one water-soluble container. 
         [0044]    In addition, it provides such a bioreactor system, further comprising enzyme means for providing at least one enzyme adapted to support the life of such at least one microbe means, wherein such at least one enzyme means is in an inert, dry state; and wherein such enzyme means is stored within such bioreactor container means. And, it provides such a bioreactor system, further comprising third water-soluble container means for containing such enzyme means in at least one water-soluble container. Further, it provides such a bioreactor system, further comprising: nutrient means for providing at least one nutrient adapted to support the life of such at least one microbe means, wherein such at least one nutrient means is in an inert, dry state; and wherein such nutrient means is stored within such bioreactor container means; enzyme means for providing at least one enzyme adapted to support the life of such at least one microbe means, wherein such at least one enzyme means is in an inert, dry state; and wherein such enzyme means is stored within such bioreactor container means. 
         [0045]    Even further, it provides such a bioreactor system, further comprising fourth water-soluble container means for containing such microbe means, such nutrient means, and such enzyme means in at least one water-soluble container. Moreover, it provides such a bioreactor system, wherein such microbe means comprises at least one bacteria. Additionally, it provides such a bioreactor system, wherein such bioreactor container means comprises at least one flexible container. Also, it provides such a bioreactor system, wherein at least one interior of such bioreactor container means, containing such microbe means, is sterile of pathogens. 
         [0046]    In accordance with another preferred embodiment hereof, this invention provides a bioreactor system, comprising: flexible container means for flexibly containing at least one fluid containing at least one cell culture; fluid input means for inputting fluid into such flexible container means; fluid output means for outputting fluid from such flexible container means; gas input means for inputting at least one gas into such flexible container means; wherein such gas input means is adapted to create bubbles in such at least one aqueous solution; gas output means for outputting at least one gas from such flexible container means. In addition, it provides such a bioreactor system, further comprising fluid input manifold means for providing fluid to such fluid input means. And, it provides such a bioreactor system, wherein such fluid input manifold means connects to a plurality of such fluid input means. 
         [0047]    Further, it provides such a bioreactor system, wherein such fluid input means comprises connector means for connecting to and disconnecting from such fluid input manifold means without tools. Even further, it provides such a bioreactor system, further comprising fluid source means for providing at least one source of at least one fluid to such fluid input manifold means. Moreover, it provides such a bioreactor system, further comprising such at least one fluid, and wherein such at least one fluid comprises sterilized water. Additionally, it provides such a bioreactor system, wherein such sterilized water is sterilized with ultraviolet radiation treatment. Also, it provides such a bioreactor system, wherein such sterilized water is sterilized by reverse osmosis treatment. In addition, it provides such a bioreactor system, wherein such fluid source means comprises temperature-controlled water. And, it provides such a bioreactor system, further comprising fluid output manifold means for receiving fluid from such fluid output means. 
         [0048]    Further, it provides such a bioreactor system, wherein such fluid output manifold means connects to a plurality of such fluid output means. Even further, it provides such a bioreactor system, wherein such fluid output means comprises connector means for quickly connecting to and disconnecting from such fluid output manifold means without tools. Moreover, it provides such a bioreactor system, further comprising gas input manifold means for providing at least one gas to such gas input means. Additionally, it provides such a bioreactor system, wherein such gas input manifold means connects to a plurality of such gas input means. Also, it provides such a bioreactor system, wherein such gas input means comprises connector means for quickly connecting to and disconnecting from such gas input manifold means without tools. In addition, it provides such a bioreactor system, further comprising gas source means for providing at least one source of gas to such gas input manifold means. 
         [0049]    And, it provides such a bioreactor system, wherein such gas source means comprises at least one gas pump. Further, it provides such a bioreactor system, further comprising atmospheric air, wherein such atmospheric air comprises such gas source means. Even further, it provides such a bioreactor system, wherein such gas source means comprises at least one oxygen generator. Moreover, it provides such a bioreactor system, wherein such gas source means comprises at least one compressed gas container. Additionally, it provides such a bioreactor system, further comprising gas output manifold means for receiving gas from such gas output means. Also, it provides such a bioreactor system, wherein such gas output manifold means connects to a plurality of such gas output means. 
         [0050]    In addition, it provides such a bioreactor system, wherein such gas output means comprises quick-connector means for quickly connecting to and disconnecting from such gas output manifold means. And, it provides such a bioreactor system, wherein such flexible container means has a volume of about 30 gallons. Further, it provides such a bioreactor system, wherein such flexible container means is opaque. Even further, it provides such a bioreactor system, further comprising rigid stackable container means for providing at least one rigid stackable container adapted to hold such flexible container means. Moreover, it provides such a bioreactor system, wherein such rigid stackable container means comprises at least one rigid stackable box. Additionally, it provides such a bioreactor system, wherein such at least one rigid stackable box comprises cardboard. 
         [0051]    Also, it provides such a bioreactor system, wherein such fluid input means, such fluid output means, such gas input means, and such gas output means are each heat-sealed to such flexible container means. In addition, it provides such a bioreactor system, wherein such fluid input means comprises at least one one-way valve. And, it provides such a bioreactor system, wherein such fluid output means comprises at least one one-way valve. Further, it provides such a bioreactor system, wherein such gas input means comprises at least one one-way valve. Even further, it provides such a bioreactor system, wherein such gas output means comprises at least one one-way valve. Moreover, it provides such a bioreactor system, wherein such fluid input means comprises at least one filter. Additionally, it provides such a bioreactor system, wherein such gas input means comprises at least one filter. Also, it provides such a bioreactor system, wherein such gas output means comprises at least one filter. In addition, it provides such a bioreactor system, wherein such gas input means comprises at least one aerator. 
         [0052]    And, it provides such a bioreactor system, further comprising controller means for controlling the contents of such flexible container means. Further, it provides such a bioreactor system, wherein such controller means controls the flow of fluid from such fluid source means to such fluid input manifold means. Even further, it provides such a bioreactor system, wherein such controller means controls the flow of fluid from such fluid input manifold means to such fluid input means. Moreover, it provides such a bioreactor system, wherein such controller means controls the flow of fluid from such fluid output means to such fluid output manifold means. Additionally, it provides such a bioreactor system, wherein such controller means controls the flow of fluid from such fluid output manifold means to at least one fluid destination. Also, it provides such a bioreactor system, wherein such controller means controls the flow of gas from such gas source means to such gas input manifold means. In addition, it provides such a bioreactor system, wherein such controller means controls the flow of gas from such gas input manifold means to such gas input means. And, it provides such a bioreactor system, wherein such controller means controls the flow of gas from such gas output means to such gas output manifold means. Further, it provides such a bioreactor system, wherein such controller means controls the flow of gas from such gas output manifold means to at least one gas destination. Even further, it provides such a bioreactor system, wherein such controller means comprises at least one programmable irrigation timer. 
         [0053]    Even further, it provides such a bioreactor system, further comprising enclosure means for enclosing at least such flexible container means. Even further, it provides such a bioreactor system, wherein such enclosure means maintains such flexible container means at a selected temperature. Even further, it provides such a bioreactor system, wherein such enclosure means comprises at least one heat pump. Even further, it provides such a bioreactor system, wherein such enclosure means comprises at least one thermostat. Even further, it provides such a bioreactor system, wherein such enclosure means is thermally insulated. Even further, it provides such a bioreactor system, wherein such enclosure means comprises fluid input manifold means for providing fluid to such fluid input means. Even further, it provides such a bioreactor system, wherein such enclosure means comprises fluid output manifold means for receiving fluid from such fluid output means. 
         [0054]    Even further, it provides such a bioreactor system, wherein such controller means comprises at least one programmable controller. Even further, it provides such a bioreactor system, wherein such temperature control means comprises at least one heat pump. Even further, it provides such a bioreactor system, wherein such temperature control means comprises at least one thermostat. Even further, it provides such a bioreactor system, further comprising gas source means for providing at least one gas to such gas input manifold means. Even further, it provides such a bioreactor system, further comprising fluid source means for providing at least one fluid to such fluid input manifold means. Even further, it provides such a bioreactor system, wherein such enclosure means encloses about eight of such at least two bioreactor chambers and a controller directs fluid to at least one of the eight bioreactor chambers to be utilized. Even further, it provides such a bioreactor system, wherein such enclosure means comprises transport means for transporting such enclosure means. Even further, it provides such a bioreactor system, wherein such transport means comprises at least one wheel. Even further, it provides such a bioreactor system, wherein such enclosure means comprises at least one access door. 
         [0055]    Even further, it provides such a bioreactor system, further comprising irrigation system means for irrigating at least one crop. Even further, it provides such a bioreactor system, further comprising irrigation system input means for inputting such fluid output from such flexible container means into such irrigation system means. Even further, it provides such a bioreactor system, wherein such irrigation system input means comprises at least one fluid pump. Even further, it provides such a bioreactor system, wherein such irrigation system input means comprises at least one venturi suction unit. Even further, it provides such a bioreactor system, wherein such irrigation system input means comprises at least one storage tank. Even further, it provides such a bioreactor system, wherein about 400 acres of crops are treated per 30 gallon flexible container. 
         [0056]    In accordance with another preferred embodiment hereof, this invention provides a bioreactor system, relating to housing and maintaining at least one bioreaction in at least two bioreactor chambers, comprising: enclosure means for enclosing such at least two bioreactor chambers; wherein such enclosure means is thermally insulated; wherein such enclosure means comprises temperature control means for controlling at least one temperature within such enclosure means; wherein such enclosure means comprises fluid input manifold means for providing at least one fluid to each of such at least two bioreactor chambers; wherein such enclosure means comprises fluid output manifold means for receiving fluid from each of such at least two bioreactor chambers; wherein such enclosure means comprises gas input manifold means for providing gas to each of such at least two bioreactor chambers; wherein such enclosure means comprises gas output manifold means for receiving gas from each of such at least two bioreactor chambers; wherein such enclosure means comprises controller means for controlling such temperature control means, such fluid input manifold means, such fluid output manifold means, such gas input manifold means, and such gas output manifold means. 
         [0057]    Even further, it provides such a bioreactor system, wherein such controller means comprises at least one programmable controller. Even further, it provides such a bioreactor system, wherein such temperature control means comprises at least one heat pump. Even further, it provides such a bioreactor system, wherein such temperature control means comprises at least one thermostat. Even further, it provides such a bioreactor system, further comprising gas source means for providing at least one gas to such gas input manifold means. Even further, it provides such a bioreactor system, further comprising fluid source means for providing at least one fluid to such fluid input manifold means. Even further, it provides such a bioreactor system, wherein such enclosure means encloses about eight of such at least two bioreactor chambers. Even further, it provides such a bioreactor system, wherein such enclosure means comprises transport means for transporting such enclosure means. Even further, it provides such a bioreactor system, wherein such transport means comprises at least one wheel. Even further, it provides such a bioreactor system, wherein such enclosure means comprises at least one access door. 
         [0058]    In accordance with another preferred embodiment hereof, this invention provides a bioreactor system, comprising: flexible container means for flexibly containing at least one aqueous solution containing at least one cell culture; fluid input means for inputting at least one fluid into such flexible container means; fluid output means for outputting such at least one aqueous solution from such flexible container means; gas input means for inputting at least one gas into such flexible container means; gas output means for outputting at least one gas from such flexible container means; enclosure means for enclosing such flexible container means; wherein such enclosure means is thermally insulated; temperature control means for controlling at least one temperature within such enclosure means; fluid input manifold means for providing at least one fluid to such fluid input means; fluid output manifold means for receiving fluid from such fluid output means; gas input manifold means for providing gas to such gas input means; gas output manifold means for receiving gas from such gas output means; and controller means for controlling such temperature control means, such fluid input manifold means, such fluid output manifold means, such gas input manifold means, and such gas output manifold means. 
         [0059]    In accordance with another preferred embodiment hereof, this invention provides each and every novel feature, element, combination, step and/or method disclosed or suggested by this provisional patent application. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWING 
         [0060]    The present invention is best understood from the following detailed description when read in conjunction with the accompanying drawing. It is emphasized that, according to common practice, the various features of the drawing are not necessarily to scale. On the contrary, the dimensions of the various features are arbitrarily expanded or reduced for clarity. Like numerals denote like features throughout the specification and drawing. 
           [0061]      FIG. 1  shows a diagram of a bioreactor system according to a preferred embodiment of the present invention. 
           [0062]      FIG. 2  shows a perspective view of a bioreactor chamber according to a preferred embodiment of the present invention. 
           [0063]      FIG. 3  shows a perspective view of the bioreactor chamber of  FIG. 2  with fluid added. 
           [0064]      FIG. 4  shows a perspective view of the bioreactor chamber of  FIG. 2  in a container. 
           [0065]      FIG. 5  shows a perspective view of the bioreactor chamber of  FIG. 2  with fluid added, in a container. 
           [0066]      FIG. 6  shows a stack of bioreactor chambers in containers. 
           [0067]      FIG. 7  shows a bioreactor housing according to a preferred embodiment of the present invention. 
           [0068]      FIG. 8  shows a diagrammatic top view of the bioreactor housing of  FIG. 7 , in use. 
           [0069]      FIG. 9  shows a diagrammatic front view of the bioreactor housing of  FIG. 7 , in use. 
           [0070]      FIG. 10  shows a cross-sectional view of diagrammatic front view of the bioreactor chamber of  FIG. 2  with fluid added, in use. 
           [0071]      FIG. 11  shows a cross-sectional view of diagrammatic front view of the bioreactor chamber of  FIG. 2  with fluid added, in use, with an aerator. 
           [0072]      FIG. 12  shows a diagram of the path of gas through the bioreactor system. 
           [0073]      FIG. 13  shows a diagram of the path of fluid through the bioreactor system. 
           [0074]      FIG. 14  shows a diagram of fluid pretreatment options. 
           [0075]      FIG. 15  shows a diagram of fluid output directly into an irrigation system. 
           [0076]      FIG. 16  shows a diagram of fluid output into other destinations. 
           [0077]      FIG. 17  shows a diagram of a method of manufacturing bioreactor chambers according to a preferred embodiment of the present invention. 
           [0078]      FIG. 18  shows a diagram of a method of using bioreactor chambers according to a preferred embodiment of the present invention. 
           [0079]      FIG. 19  shows a diagram of a method of distributing bioreactor chambers according to a preferred embodiment of the present invention. 
           [0080]      FIG. 20  shows a diagram of a method of farming using bioreactor chambers according to a preferred embodiment of the present invention. 
           [0081]      FIG. 21  shows a diagram of additional interfaces to the controller. 
       
    
    
     DETAILED DESCRIPTION 
       [0082]      FIG. 1  shows a diagram of a bioreactor system  100  according to a preferred embodiment of the present invention; and  FIG. 1  is further amplified and explained in reference to  FIGS. 2-11  hereof. Preferably, bioreactor system  100  comprises bioreactor  102 , as shown. Preferably, bioreactor  102  provides an apparatus for automatic serial batch production of desired live microbes  101 . Preferably, bioreactor  102  comprises one or more bioreactor chambers  105 , fluid source  110 , fluid input manifold  115 , gas source  120 , gas input manifold  125 , gas destination  130 , gas output manifold  135 , fluid destination  140 , fluid output manifold  145 , and at least one controller  150 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, intended use, etc., other bioreactor components, such as sensors, sound generators, stirrers, starter culture injectors, light sources, fewer or more sources, inputs, outputs, manifolds or controllers, etc., may suffice. 
         [0083]    For the purposes of this patent application, “microbes” are defined as organisms and/or cell cultures (capable of being grown in a bioreactor), such as, for example, bacteria, protazoa, yeasts, fungi, molds, algae, nematodes, mammalian cells, insect cells, other animal cells, plant cells, adherent cells on microcarriers, etc. Typically, for the agricultural purposes used as examples herein, “microbes” refers to aerobic bacteria. 
         [0084]    Preferably, fluid source  110  comprises a source of fluid  112 , suitable to supporting bioreactions, preferably sterilized water  114 , as shown. Preferably, fluid source  110  provides sterile pH adjusted water adapted to support the maximum reproductive rate of particular microbes, such as, for example, a pH of between about 7 and about 6 for most aerobic bacteria, adjusted by means such as, for example, bubbling carbon dioxide through the water, as is known in the art of microbiology. Preferably, fluid source  110  provides temperature-controlled fluid  112 , preferably at about 80 degrees Fahrenheit, so that fluid  112  entering bioreactor chambers  105  is the correct temperature for efficient microbial growth. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, microbe species, desired growth rate, etc., other temperatures, such as 33 degrees Fahrenheit, 50 degrees Fahrenheit, 90 degrees Fahrenheit, etc., may suffice. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, microbe requirements, etc., other fluids, such as mixtures of water and other chemicals, and such as glucose solution, liquid agar, seawater, etc., may suffice. 
         [0085]    For the purposes of this patent application, a “bioreaction” means the growth and reproduction of live microbes  101  in bioreactor  102 . For aerobic bacteria, water, oxygen, and nutrients are typically required for bioreaction; usually, a temperature of about 80 degrees Fahrenheit is preferred for efficient growth. 
         [0086]    Preferably, fluid input manifold  115  distributes fluid  112  from fluid source  110  to each bioreactor chamber  105 , as shown. Preferably, fluid input manifold  115  comprises one valve  117  for each bioreactor chamber  105 , as shown. Preferably, each valve  117  is individually controlled by such at least one controller  150 , so that fluid  112  may be individually supplied to each bioreactor chamber  105 , as shown. 
         [0087]    Preferably, gas source  120  comprises at least one source of gas  122  suitable to supporting bioreactions, as shown, such as, for example, oxygen. Preferably, gas  122  comprises atmospheric air  124 . Preferably, gas source  120  provides gas  122  at sufficient pressure to bubble through fluid  112  in bioreactor chamber  105  (as shown in  FIGS. 10 and 11 ). Preferably, gas source  122  is controlled by such at least one controller  150 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, microbe species, etc., other gasses, such as synthetic oxygen/gas mixes, hydrogen sulfide, methane, etc., may suffice. 
         [0088]    Preferably, gas input manifold  125  distributes gas  122  from gas source  120  to each bioreactor chamber  105 , as shown. Preferably, gas input manifold  125  comprises one valve  127  for each bioreactor chamber  105 , as shown. Preferably, each valve  127  is individually controlled by controller  150 , so that gas  122  may be individually supplied to each bioreactor chamber  105 , as shown. 
         [0089]    Preferably, gas output manifold  135  receives gas  122  (typically enriched in carbon dioxide by the bioreaction) from each bioreactor chamber  105 , as shown. Preferably, gas output manifold  135  comprises one valve  137  for each bioreactor chamber  105 , as shown. Preferably, each valve  137  is individually controlled by the at least one controller  150 , so that gas  122  may be individually released from each bioreactor chamber  105  (and/or held in each bioreactor chamber  105 ), as shown. Preferably, each valve  137  also serves as a gas relief valve  138 , which automatically relieves serious excess gas  122  pressure in bioreactor chamber  105  while maintaining headspace  123 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, intended use, etc., other arrangements, such as a separate gas relief valve attached to the bioreactor chamber, a single gas relief valve attached to the gas output manifold, a gas relief valve in each gas input tube, etc., may suffice. 
         [0090]    Preferably, gas destination  130  receives gas  122  from gas output manifold  135 , as shown. Preferably, gas destination  130  vents gas  122  to the atmosphere. Preferably, where suitable, gas destination  130  is controlled by the at least one controller  150 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, type of gas, etc., other arrangements, such as venting into a container, venting into a greenhouse, etc., may suffice. 
         [0091]    Preferably, fluid output manifold  145  receives fluid  112  (typically enriched in live microbes  101  by the bioreaction) from each bioreactor chamber  105 , as shown. Preferably, fluid output manifold  145  comprises one valve  147  for each bioreactor chamber  105 , as shown. Preferably, each valve  147  is individually controlled by the at least one controller  150 , so that fluid  122  may be individually released from each bioreactor chamber  105  and/or held in each bioreactor chamber  105 , i.e. the controller  150  may control the valves  147  such that fluid  122  may be held in one bioreactor chamber  105  while fluid  122  is released from another bioreactor chamber  105 , as shown. 
         [0092]    Preferably, fluid destination  140  receives fluid  112  from fluid output manifold  145 , as shown. Preferably, fluid output manifold  145  is connected to pump  148 , as shown in  FIG. 7 . Preferably, pump  148  is controlled by the at least one controller  150 , as shown in  FIG. 7 , so that fluid  122  may be pumped from fluid output manifold  145  at a controlled rate. Preferably, fluid destination  140  delivers fluid  112  where it is needed to be used, such as, for example, directly into an irrigation system  285 , as shown in  FIG. 15 . 
         [0093]    Preferably, fluid input manifold  115 , gas input manifold  125 , gas output manifold  135 , and fluid output manifold  145  are reusable and sterilizable. More preferably, fluid input manifold  115 , gas input manifold  125 , gas output manifold  135 , and fluid output manifold  145  are reusable and autoclavable. Most preferably, fluid input manifold  115 , gas input manifold  125 , gas output manifold  135 , and fluid output manifold  145  are stainless steel. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other manifold materials, such as disposable plastic tubing, molded plastic, other metals, etc., may suffice. 
         [0094]    Preferably, the at least one controller  150  comprises at least one programmable electronic controller  152 , as shown, such as, for example, an irrigation timer  154 , as shown. Preferably, the at least one controller  150  is programmable to add fluid  112  and gas  122  to one bioreactor chamber  105  after another (and to release fluid  112  and gas  122  from the bioreactor chamber  105 ), as shown, by opening and closing the valves (preferably solenoid-controlled valves) on the manifolds, on a timed schedule programmable by a user. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other controllers, such as computers, multiple timers, a single controller controlling multiple bioreactors, etc., may suffice. 
         [0095]      FIG. 2  shows a perspective view of a bioreactor chamber  105  according to a preferred embodiment of the present invention. Preferably, bioreactor chamber  105  comprises a sterile, disposable, flexible container, as shown. Preferably, bioreactor chamber  105  comprises bag  160 , fluid input  170 , gas input  180 , gas output  190 , and fluid output  200 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other bioreactor chamber arrangements, such as other input and/or output ports, multiple compartments, rigid containers, other input and/or output port placements, etc., may suffice. 
         [0096]    Preferably, bag  160  comprises at least one strong, flexible, plastic bag, preferably about three feet wide by about three feet long, with a useful volume of about 50 gallons, having a top surface  162  and a bottom surface  163 , sealed on all four edges  161 , as shown. Preferably, for culturing soil bacteria, bag  160  is opaque to protect live microbes  101  from accidental exposure to light during bioreaction. Preferably, the interior  164  of bag  160  is sterile prior to use. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, intended use, etc., other bag sizes, such as one-half gallon, ten gallons, five hundred gallons, and other bag shapes, etc., may suffice. 
         [0097]    For the purposes of this patent application, the term “sterile” means the absence of live (or dormant) pathogens, such as, for example, unwanted anaerobic bacteria. Bioreactors  105  may be sterilized by heat and/or radiation prior to inserting inert microbes  210 . A bioreactor  105  containing only desired live microbes  101 , or only desired inert microbes  210 , has a “sterile” interior  164 . For the purposes of this patent application, it should be understood that inert microbes  210  may be in various states such as substantially wet or substantially dry, even though the following exemplary embodiments often and generally refer to the embodiment in which the inert microbes are substantially dry, inert microbes. 
         [0098]    Preferably, fluid input  170  comprises a flexible plastic tube  171 , as shown, which is preferably heat-sealed into one edge  161  of bag  160  at the first end  172 , as shown. Preferably, the second end  173  comprises a connector  174 , adapted to sealably connect to a valve  117  on fluid input manifold  115  without the need for tools, as shown. Connectors of this type, commonly called “quick-release connectors”, are well known in the art of laboratory gas and liquid manifolds. Preferably, fluid input  170  comprises a filter  175  to further purify fluid  122  going into bag  160 , as shown. Preferably, fluid input  170  comprises one-way valve  176 , which is preferably adapted to permit fluid  122  to flow from second end  173  to first end  172 , while preventing fluid  122  from flowing in the other direction, as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other arrangements, such as no one-way valve, no filter, a quick connector between the fluid input and the bag, etc., may suffice. 
         [0099]    Preferably, gas input  180  comprises a flexible plastic tube  181 , as shown, which is preferably heat-sealed into one edge  161  of bag  160  at the first end  182 , as shown. Preferably, the second end  183  comprises a connector  184 , adapted to sealably connect to a valve  118  on gas input manifold  125 , as shown, without the need for tools. Preferably, gas input  180  comprises a filter  185  to further purify gas  122  going into bag  160 , as shown. Preferably, gas input  180  comprises one-way valve  186 , which is preferably adapted to permit gas  122  to flow from second end  183  to first end  182 , while preventing gas  122  from flowing in the other direction, as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other arrangements, such as no one-way valve, no filter, a quick connector between the gas input and the bag, etc., may suffice. 
         [0100]    Preferably, gas output  190  comprises a flexible plastic tube  191 , as shown, which is preferably heat-sealed into the top surface  162  of bag  160  at the first end  192 , as shown. Preferably, the second end  193  comprises a connector  194 , adapted to sealably connect to a valve  137  on gas output manifold  135 , as shown, without the need for tools. Preferably, gas output  190  comprises a filter  195  to purify gas  122  leaving bag  160 , as shown. Preferably, gas output  190  comprises one-way valve  196 , which is preferably adapted to permit gas  122  to flow from first end  192  to second end  193 , while preventing gas  122  from flowing in the other direction, as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other arrangements, such as no one-way valve, no filter, a quick connector between the gas output and the bag, etc., may suffice. 
         [0101]    Preferably, fluid output  200  comprises a flexible plastic tube  201 , as shown, which is preferably heat-sealed into one edge  161  of bag  160  at the first end  202 , as shown. Preferably, the second end  203  comprises a connector  204 , adapted to sealably connect to a valve  147  on fluid output manifold  145 , as shown, without the need for tools. Preferably, fluid output  200  comprises one-way valve  206 , which is preferably adapted to permit fluid  122  to flow from first end  202  to second end  203 , while preventing fluid  122  from flowing in the other direction, as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other arrangements, such as no one-way valve, a filter, a quick connector between the fluid output and the bag, etc., may suffice. 
         [0102]    Preferably, prior to use, bag  160  is sterilized and is then preloaded with at least one of inert microbes  210 , dry, inert nutrient  215 , and dry, inert enzymes  220 , as shown. Preferably, dry, inert microbes  210 , dry, inert nutrient  215 , and dry, inert enzymes  220  are contained in water-soluble capsules  225 , as shown. Preferably, water-soluble capsules  225  comprise polyvinyl alcohol plastic or other water-soluble plastics known in the art of packaging. Preferably, water-soluble capsules  225  are placed into bag  160  prior to adding fluid  112  to bag  160 , as shown, preferably prior to bag  160  being stored prior to adding fluid  112 , most preferably prior to bag  160  being shipped to the user prior to adding fluid  112 . Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other water-soluble capsules, such as other water-soluble materials, other water-soluble container types, water-soluble capsules with a known dissolution time delay or rate, etc., may suffice. 
         [0103]    Preferably, when fluid  112  is added to bag  160 , water-soluble capsules  225  dissolve, releasing dry, inert microbes  210 , dry, inert nutrient  215 , and dry, inert enzymes  220  into fluid  112 , as shown. At this point, dry, inert microbes  210 , dry, inert nutrient  215 , and dry, inert enzymes  220  rehydrate and become live microbes  101 , available nutrient  216 , and/or activated enzymes  221  (as shown in  FIG. 3 ), which proceed to bioreact (i.e., the live microbes  101  grow and multiply), resulting in an increased number of live microbes  101 . Preferably, a useful quantity of live microbes  101  is generated within eight to twelve hours of adding fluid  112  to bag  160 , depending on the species of live microbes  101 , the temperature of fluid  112 , the type and quantity of nutrient  215 , etc. In other exemplary embodiments, other shorter or longer times such as a twenty hour time period, may be used. 
         [0104]    Preferably, the end-user maintains a bioreactor  102  on the end-user&#39;s own site to provide an on-demand source of live microbes  101 . By providing disposable bioreactor chambers  105  to end-users in a sterile, preloaded condition, ready for immediate use, the end-user is relieved from having to maintain a highly sterile environment wherein to load bioreactor chambers  105  with beneficial microbe starter cultures. Also, the end-user is relieved from most of the difficulty of sterilizing the bioreactor between uses (to prevent the growth of pathogenic microbes) as the disposable bioreactor chambers  105  are simply replaced. These conveniences are particularly useful to agricultural end-users. 
         [0105]    A large variety of microbes (particularly bacteria) have been identified in the art as providing useful bioremediation effects when applied live in sufficient concentrations to soil, plants, and waterways. Preferably, the inert microbes  210 , dry, inert nutrient  215 , and/or dry, inert enzymes  220  are selected and loaded into bioreactor chambers  105  to meet the bioremediation needs of the particular end-user, which needs will vary depending on such factors as, for example, soil type and condition, type of crops (or turf), types of local pathogens, time of year, etc. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other bioreactor chamber contents, such as antibodies, trace nutrients, etc., may suffice. 
         [0106]      FIG. 3  shows a perspective view of bioreactor chamber  105  of  FIG. 2  with fluid  112  added. Preferably, gas  122  and fluid  112  are added to bioreactor chamber  105  to start the bioreaction, as shown. Preferably, additional gas  122  is added to bioreactor chamber  105  on a schedule determined by the user to meet the metabolic needs of live microbes  101 , such as, for example, continuously, for fifteen minutes per hour, only on bioreaction start-up, etc. Typically, it is not necessary to add additional fluid  112 . 
         [0107]    Preferably, a headspace  123  of gas  122  is maintained adjacent top surface  162  of bag  160 , as shown, to provide a gas exchange interface with fluid  112 , so that oxygen from gas  122  may be absorbed by fluid  112 , and carbon dioxide generated by the bioreaction in fluid  112  may be released into headspace  123  (in the case of aerobic microbes). Preferably, gas relief valve  138  maintains the pressure required for headspace  123 , as shown, without permitting the pressure to build up to undesirable levels (such as where bag  160  may burst). Preferably, valve  137  on gas output manifold  135  is open for active and used bioreactor chambers  105  (and gas relief valve  138  is relied on to control gas output), and is closed for unused bioreactor chambers  105  (to prevent contamination of the unused bioreactor chambers  105 ). 
         [0108]      FIG. 4  shows a perspective view of the bioreactor chamber  105  of  FIG. 2  in a container  230 . Preferably, bioreactor chambers  105  are held and supported during use in containers  230 , as shown. Preferably, containers  230  are strong, stackable bins, about three feet wide by about three feet long by about nine inches deep, capable of supporting at least about three hundred and eighty pounds of fluid  122  (for 50-gallon bags  160 , with about 45 gallons of fluid and about five gallons of headspace  123 ) in bioreactor chambers  105 , as shown. Preferably, containers  230  comprise openings  232  adapted to permit the passage of fluid input  170 , gas input  180 , gas output  190 , and fluid output  200 , as shown. Preferably, containers  230  are reusable, such as, for example, sturdy plastic bins, as shown. In an alternate preferred embodiment, containers  230  are disposable, such as, for example, sturdy cardboard boxes. 
         [0109]      FIG. 5  shows a perspective view of bioreactor chamber  105  of  FIG. 2  with fluid  112  added, in a container  230 . Preferably, containers  230  are tall enough to accommodate bioreactor chambers  105  in use, as shown. 
         [0110]      FIG. 6  shows a stack of bioreactor chambers  105  in containers  230 . Preferably, multiple bioreactor chambers  105  in containers  230  are stacked to conserve space in bioreactor  102 , as shown. Preferably, the multiple stacked bioreactor chambers  105  in containers  230  are hooked up to fluid input manifold  115 , gas input manifold  125 , gas output manifold  135 , and fluid output manifold  145 , as shown, and are automatically filled, incubated (i.e., bioreacted), and emptied one after the other according to the programming of controller  150 . In an alternate preferred embodiment, depending on the strength of containers  230 , multiple stacked bioreactor chambers  105  in containers  230  may be filled and incubated simultaneously. 
         [0111]      FIG. 7  shows a bioreactor housing  250  according to a preferred embodiment of the present invention. Preferably, bioreactor  102  comprises housing  250 , as shown. Preferably, housing  250  supports and contains at least fluid input manifold  115 , gas input manifold  125 , gas output manifold  135 , fluid output manifold  145 , and controller  150 , as shown. Preferably, housing  250  also supports and contains pump  148 , power source  255 , and temperature regulator  260 , as shown. Preferably, depending on the type of gas source  120 , housing  250  also supports and contains gas source  120 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other housing contents, such as sensors, lights, sound generators, other manifolds, etc., may suffice. 
         [0112]    Preferably, housing  250  comprises enclosure  265 , enclosure  270 , and doors  275 , as shown. Preferably, enclosure  265  comprises an insulated, lightproof enclosure, into which multiple stacked bioreactor chambers  105  in containers  230  may be placed, as shown. Preferably, enclosure  265  contains or provides access to fluid input manifold  115 , gas input manifold  125 , gas output manifold  135 , and fluid output manifold  145 , as shown. 
         [0113]    Preferably, enclosure  265  comprises floor  266 , as shown. Preferably, floor  266  is raised over the bottom of enclosure  265  to provide a space for the wiring  267  between controller  150  and each manifold, for pump  148 , for the wiring  268  between pump  148  and controller  150 , and for the wiring  269  between pump  148  and power source  255 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other arrangements, such as no raised floor, other wiring, etc., may suffice. 
         [0114]    Preferably, enclosure  270  comprises an air-vented enclosure, preferably on the back of housing  250 , as shown. Preferably, enclosure  270  contains and supports controller  150 , power source  255 , and temperature regulator  260 , as shown. Preferably, depending on the type of gas source  120 , enclosure  270  also supports and contains gas source  120 , as shown. Preferably, enclosure  270  comprises door  271 , as shown, for maintenance access. 
         [0115]    Preferably, power source  255  supplies electrical power to at least pump  148 , controller  150 , and temperature regulator  260 , as shown. Preferably, depending on the type of gas source  120  (such as where gas source  120  is an air pump, as shown), power source  255  also supplies electrical power to gas source  120 , as shown. Preferably, power source  255  plugs into a conventional power outlet. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other power source arrangements, such as solar power, battery power, etc., may suffice. 
         [0116]    Preferably, temperature regulator  260  maintains a selected temperature in enclosure  265 , such as, for example, a temperature of about eighty degrees Fahrenheit. Preferably, temperature regulator  260  comprises an electrical appliance capable of providing both heated and cooled air to enclosure  265 , as shown, of the sort known in the art of heating and cooling appliances, such as, for example, a heat pump  261 , as shown. Preferably, temperature regulator  260  comprises thermostat  262 , as shown, which senses the temperature in enclosure  265 . Preferably, temperature regulator  260  is programmable. More preferably, temperature regulator  260  is controlled by controller  150 , as shown, which is preferably programmable. Preferably, where live microbes  101  are stored and used over multiple days, temperature regulator  260  may decrease the temperature inside housing  250  to lower the metabolic rate of live microbes  101  between uses. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other temperature regulators, such as a Peltier junction, separate heaters and coolers, individual temperature regulators for each bioreactor chamber, etc., may suffice. 
         [0117]    Preferably, housing  250  comprises heavy-duty casters  276 , so that housing  250  may be easily moved. 
         [0118]      FIG. 8  shows a diagrammatic top view of the bioreactor housing  250  of  FIG. 7 , in use. 
         [0119]      FIG. 9  shows a diagrammatic front view of the bioreactor housing  250  of  FIG. 7 , in use. Preferably, a stack of bioreactor chambers  105  in containers  230  are installed in housing  250 , as shown. Preferably, bioreactor  102  then runs automatically, according to the programming of controller  150 , serially incubating and emptying each bioreactor chamber  105  in turn, as shown. Preferably, when every bioreactor chamber  105  in the stack has been used and emptied, the user removes the used bioreactor chambers  105  and installs new bioreactor chambers  105 . Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other arrangements, such as manual operation, incubating multiple bioreactor chambers simultaneously, etc., may suffice. 
         [0120]      FIG. 10  shows a cross-sectional view of the bioreactor chamber  105  of  FIG. 2  with fluid  112  added, in use. Preferably, gas  122  bubbles into fluid  112  in bag  160  from gas input  180 , as shown, disturbing the interface between fluid  112  and headspace  123 , as shown, facilitating gas exchange between fluid  112  and headspace  123 . 
         [0121]      FIG. 11  shows a cross-sectional view of diagrammatic front view of the bioreactor chamber of  FIG. 2  with fluid  112  added, in use, with an aerator  187 . In an alternate preferred embodiment, gas  122  bubbles into fluid  112  in bag  160  from aerator  187 , as shown, disturbing the interface between fluid  112  and headspace  123 , as shown, facilitating gas exchange between fluid  112  and headspace  123 . Preferably, bioreactor chamber  105  comprises aerator  187 , as shown, which comprises a gas diffuser of the sort known in the art of aeration, such as, for example, a reusable steel air manifold, a disposable ceramic fish tank type aerator, a disposable flexible plastic fish tank type aerator (as shown), etc. 
         [0122]      FIG. 12  shows a diagram of the path of gas  122  through bioreactor  102 . Preferably, gas source  120  comprises an apparatus such as, for example, a gas pump  121 , as shown, a compressed gas cylinder, an oxygen generator, etc. Preferably, there is a filter  119  between gas source  120  and gas input manifold  125 . Preferably, filter  119  is adapted to filter microbes out of gas  122 . Preferably, gas source  120  comprises a source of gas  122  suitable to supporting bioreactions, such as, for example, atmospheric air  124 , as shown, nitrogen/oxygen mixtures, pure oxygen, etc. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other gas sources, such as remote air pumps, bellows, etc., may suffice. 
         [0123]      FIG. 13  shows a diagram of the path of fluid  112  through bioreactor  102 . 
         [0124]      FIG. 14  shows a diagram of fluid  112  pretreatment options. Preferably, fluid source  110  comprises a source of fluid  112  suitable to supporting bioreactions, preferably a source of sterilized water  114 , as shown. Preferably, fluid source  110  comprises a sterilizer  111  such as, for example, a temperature controlled tank, a reverse-osmosis system, a deionization system, etc. Preferably, fluid source  110  is controlled by controller  150 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other fluid sources, such as municipal water pipes, fluid pumped from a container, etc., may suffice, as shown. 
         [0125]    Preferably, water  114  is sterilized by sterilizer  111 , either in fluid source  110  or by another apparatus prior to reaching fluid input manifold  115 , as by at least one of distillation  280 , reverse osmosis  281 , sonification  282 , and/or ultraviolet light treatment  283 , as shown. Preferably, where water  114  is turbid (i.e., from a city water supply, from a lake, etc.), sonification  282  and ultraviolet light  283  are used together to provide complete sterilization. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other methods of sterilizing water, such as radiation, heat, deionization, chemical treatment, etc., may suffice. 
         [0126]      FIG. 15  shows a diagram of fluid  112  output directly into an irrigation system  285 . Preferably, fluid destination  140  delivers fluid  112  to the required point of use or storage, as shown. Preferably, fluid destination  140  delivers fluid  112  directly into irrigation system  285 , as shown, which then delivers fluid  112  to crops including leaves and stems thereof, soil, etc. Preferably, fluid destination  140  comprises an apparatus such as, for example, a pump, a venturi suction device, a drain into an irrigation water tank, etc. Preferably, fluid destination  140  is controlled by controller  150 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other fluid outputs, such as a fluid storage device, a freezer, etc., may suffice. 
         [0127]    Preferably, where live microbes  101  comprise soil bacteria, live microbes  101  are protected from light (especially ultraviolet light) by irrigating at night. Preferably, the live microbe  101  contents of one incubated 30-gallon bioreactor chamber  105  are added to irrigation water to treat about four hundred acres of crops or turf, typically on an about weekly basis. Preferably, a single bioreactor chamber  105  may be used over multiple days until it is emptied, depending of the amount of time it takes to irrigate four hundred acres. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other treatment levels, such as daily, monthly, yearly, higher concentration of application, lower concentration of application, etc., may suffice. 
         [0128]      FIG. 16  shows a diagram of fluid  112  output into other destinations. Preferably, fluid destination  140  delivers fluid  112  to an irrigation tank  286 , on, for example, a sprayer truck  287 , a sprayer airplane  288 , etc, as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other fluid destinations, such as watering cans, irrigation ponds, etc., may suffice. 
         [0129]      FIG. 17  shows a diagram of a method  300  of manufacturing bioreactor chambers  105  according to a preferred embodiment of the present invention. Preferably, bioreactor system  100  comprises method  300 , as shown. Preferably, method  300  comprises the steps of: selecting (step  302 ) at least one dry, inert microbe  210 ; selecting (step  304 ) at least one dry, inert nutrient  215  adapted to support the life of such dry, inert microbe  210 ; placing (step  306 ) such dry, inert microbe  210  and such dry, inert nutrient  215  into at least one sterile bioreactor chamber  105 ; and storing (step  308 ) such sterile bioreactor chamber  105  containing such dry, inert microbe  210  and such dry, inert nutrient  215 , as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other steps, such as sterilizing the bioreactor chamber prior to placing the microbes inside, closing the inputs and outputs to maintain sterility, packaging the loaded bioreactor chamber in a sterile container, etc., may suffice. 
         [0130]    Preferably, method  300  further comprises the step of shipping (step  310 ) such sterile bioreactor chamber  105  containing such dry, inert microbe  210  and such dry, inert nutrient  215  to at least one user, as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other steps, such as accepting an order for a preloaded bioreactor, receiving a promise of payment for the preloaded bioreactor, setting up automatic timed shipments of preloaded bioreactors, etc., may suffice. 
         [0131]    Preferably, method  300  further comprises the steps of: adding (step  312 ) water  114  to such sterile bioreactor chamber  105  containing such dry, inert microbe  210  and such dry, inert nutrient  215 ; and permitting (step  314 ) at least one bioreaction to occur, as shown. 
         [0132]    Preferably, method  300  further comprises the step of placing (step  316 ) such dry, inert microbes  210  into at least one water-soluble container  225  prior to placing (step  306 ) such dry, inert microbes  210  into such sterile bioreactor chamber  105 , as shown. Preferably, dry, inert microbes  210  are packaged into water-soluble containers  225  in a sterile environment. Preferably, the filled water-soluble containers  225  are inserted into bioreactor chamber  105  in a sterile environment, such as, for example, a cleanroom, a laboratory, etc. Preferably, the filled water-soluble containers  225  are inserted into bioreactor chamber  105  through at least one of fluid input  170 , gas input  180 , gas output  190 , and fluid output  200 . Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other methods of loading dry, inert microbes into the bioreactor chambers, such as a separate sealable port in the bioreactor chamber, manufacturing the bioreactor chambers with the dry, inert microbes already inside, manufacturing the bioreactor chambers with a water-soluble film compartment containing the dry, inert microbes, etc., may suffice. 
         [0133]    Preferably, method  300  further comprises the step of placing (step  318 ) such dry, inert nutrient  215  into water-soluble container  225  prior to placing (step  306 ) such dry, inert nutrient  215  into such sterile bioreactor chamber  105 , as shown. 
         [0134]    Preferably, method  300  further comprises the steps of: selecting (step  320 ) at least one dry, inert enzyme  220  adapted to support the life of such dry, inert microbe  210 ; and placing (step  322 ) such dry, inert enzymes  220  into such sterile bioreactor chamber  105 , as shown. Preferably, method  300  further comprises the step of placing (step  324 ) such dry, inert enzymes  220  into at least one water-soluble container  225  prior to placing (step  322 ) such dry, inert enzymes  220  into such sterile bioreactor chamber  105 , as shown. 
         [0135]      FIG. 18  shows a diagram of a method  330  of using bioreactor chambers  105  according to a preferred embodiment of the present invention. Preferably, bioreactor system  100  comprises method  330 , as shown. Preferably, method  330  comprises the steps of: selecting (step  332 ) at least one dry, inert microbe  210 ; selecting (step  334 ) at least one dry, inert nutrient  215  adapted to support the life of such dry, inert microbe  210 ; selecting (step  336 ) at least one previously sterilized bioreactor chamber  105 ; receiving (step  338 ) such bioreactor chamber  105  containing such dry, inert microbe  210  and such dry, inert nutrient  215  from at least one manufacturer; installing (step  340 ) such bioreactor chamber  105  containing such dry, inert microbe  210  and such dry, inert nutrient  215  in at least one bioreactor  102 ; adding (step  342 ) fluid  112  (i.e. water  114 ) to such bioreactor chamber  105  containing such dry, inert microbe  210  and such dry, inert nutrient  215 ; waiting (step  346 ) for at least one bioreaction in such bioreactor chamber  105  to generate a useful number of live, active microbes  101 ; and harvesting (step  348 ) such useful number of live, active microbes  101 , as shown. 
         [0136]    Preferably, method  330  further comprises the step of adding (step  344 ) oxygen (i.e. gas  122 ) to such bioreactor chamber  105 , after such step of adding (step  342 ) fluid  112  to such bioreactor chamber  105 , as shown. 
         [0137]    Preferably, the step of harvesting (step  348 ) such useful number of live, active microbe  101  comprises the step of adding (step  350 ) such useful number of live, active microbes  101  into at least one irrigation system  285 , as shown. 
         [0138]    Preferably, method  330  further comprises the steps of: uninstalling (step  352 ) such bioreactor chamber  105 ; and disposing (step  354 ) of such bioreactor chamber  105 , after the step of harvesting (step  348 ) such useful number of live, active microbes  101 , as shown. 
         [0139]    An example of the use of controller  150  to several of the steps of method  330  is described here. To prepare a single batch of live microbes  101 , having an eight-hour incubation period, to dispense into irrigation system  285  at midnight, the following timed sequence may be used (all valves start closed): 
         [0000]    
       
         
               
               
               
             
           
               
                   
               
             
             
               
                  4:00 pm 
                 Open one valve 117 on fluid input 
                 Adds fluid 112 to one bioreactor 
               
               
                   
                 manifold 115 
                 chamber 105 
               
               
                  4:00 pm 
                 Open one valve 127 on gas input 
                 Adds gas to same bioreactor 
               
               
                   
                 manifold 125 
                 chamber 105 
               
               
                  4:00 pm 
                 Open one valve 137 on gas output 
                 Releases gas 122 from same 
               
               
                   
                 manifold 135 
                 bioreactor chamber 105, rate 
               
               
                   
                   
                 controlled by gas relief valve 138 
               
               
                  4:30 pm 
                 Close valve 117 on fluid input 
                 Shuts off fluid 112 going to same 
               
               
                   
                 manifold 115 
                 bioreactor chamber 105 (timed to 
               
               
                   
                   
                 add about 30 gallons) 
               
               
                 12:00 am 
                 Open valve 147 on fluid output 
                 Releases fluid 112 with live 
               
               
                   
                 manifold 145 
                 microbes 101 from same 
               
               
                   
                   
                 bioreactor chamber 105 into fluid 
               
               
                   
                   
                 output manifold 145 
               
               
                 12:00 am 
                 Activate pump 148 at programmed flow 
                 Moves fluid 112 from fluid output 
               
               
                   
                 rate 
                 manifold 145 to fluid destination 
               
               
                   
                   
                 140 
               
               
                 12:00 am 
                 Close same valve 127 on gas input 
                 Shuts off gas 122 to same 
               
               
                   
                 manifold 125 
                 bioreactor 105 
               
               
                   
               
             
          
         
       
     
         [0140]    To prepare a two batches of live microbes  101 , having an eight-hour incubation period, to dispense 30 gallons of fluid  112  into irrigation system  285  at midnight for three nights, the following timed sequence may be used (all valves start closed) according to an exemplary sequence in which microbes are growing/reacting in a second bioreactor chamber while previously reacted/grown microbes are being discharged from a first bioreactor chamber via a manifold: 
         [0000]    
       
         
               
               
               
             
           
               
                   
               
             
             
               
                 4:00 pm 
                 Open first valve 117 on fluid input 
                 Adds fluid 112 to first bioreactor 
               
               
                 1st day 
                 manifold 115 
                 chamber 105 
               
               
                 4:00 pm 
                 Open first valve 127 on gas input 
                 Adds gas to first bioreactor 
               
               
                 1st day 
                 manifold 125 
                 chamber 105 
               
               
                 4:00 pm 
                 Open first valve 137 on gas output 
                 Releases gas 122 from first 
               
               
                 1st day 
                 manifold 135 
                 bioreactor chamber 105, rate 
               
               
                   
                   
                 controlled by gas relief valve 138 
               
               
                 4:30 pm 
                 Close first valve 117 on fluid input 
                 Shuts off fluid 112 going to first 
               
               
                 1st day 
                 manifold 115 
                 bioreactor chamber 105 (timed to 
               
               
                   
                   
                 add about 45 gallons) 
               
               
                 12:00 am 
                 Open first valve 147 on fluid output 
                 Releases fluid 112 with live 
               
               
                 1st day 
                 manifold 145 
                 microbes 101 from first bioreactor 
               
               
                   
                   
                 chamber 105 into fluid output 
               
               
                   
                   
                 manifold 145 
               
               
                 12:00 am 
                 Activate pump 148 at programmed 
                 Moves 30 gallons of fluid 112 from 
               
               
                 1st day 
                 flow rate 
                 fluid output manifold 145 to fluid 
               
               
                   
                   
                 destination 140 
               
               
                 12:20 am 
                 Deactivate pump 148 
                 Timed to leave 15 gallons of fluid 
               
               
                 1st day 
                   
                 112 in first bioreactor 105 
               
               
                 4:00 pm 
                 Open second valve 117 on fluid input 
                 Adds fluid 112 to second bioreactor 
               
               
                 2nd day 
                 manifold 115 
                 chamber 105 
               
               
                 4:00 pm 
                 Open second valve 127 on gas input 
                 Adds gas to second bioreactor 
               
               
                 2nd day 
                 manifold 125 
                 chamber 105 
               
               
                 4:00 pm 
                 Open second valve 137 on gas 
                 Releases gas 122 from second 
               
               
                 2nd day 
                 output manifold 135 
                 bioreactor chamber 105, rate 
               
               
                   
                   
                 controlled by gas relief valve 138 
               
               
                 4:30 pm 
                 Close second valve 117 on fluid input 
                 Shuts off fluid 112 going to second 
               
               
                 2nd day 
                 manifold 115 
                 bioreactor chamber 105 (timed to 
               
               
                   
                   
                 add about 45 gallons) 
               
               
                 12:00 am 
                 Activate pump 148 at programmed 
                 Moves 15 gallons of fluid 112 from 
               
               
                 2nd day 
                 flow rate 
                 fluid output manifold 145 to fluid 
               
               
                   
                   
                 destination 140 (from first 
               
               
                   
                   
                 bioreactor 105) 
               
               
                 12:00 am 
                 Close first valve 127 on gas input 
                 Shuts off gas 122 to first bioreactor 
               
               
                 2nd day 
                 manifold 125 
                 105 
               
               
                 12:10 am 
                 Open second valve 147 on fluid 
                 Releases fluid 1 !2 with live 
               
               
                 2nd day 
                 output manifold 145 
                 microbes 101 from second 
               
               
                   
                   
                 bioreactor chamber 105 into fluid 
               
               
                   
                   
                 output manifold 145 (first bioreactor 
               
               
                   
                   
                 is empty) 
               
               
                 12:10 am 
                 Continue pump 148 at programmed 
                 Moves 15 gallons of fluid 112 from 
               
               
                 2nd day 
                 flow rate 
                 fluid output manifold 145 to fluid 
               
               
                   
                   
                 destination 140 (from second 
               
               
                   
                   
                 bioreactor 105) 
               
               
                 12:20 am 
                 Deactivate pump 148 
                 Timed to leave 30 gallons of fluid 
               
               
                 2nd day 
                   
                 112 in second bioreactor 105 
               
               
                 12:00 am 
                 Activate pump 148 at programmed 
                 Moves 30 gallons of fluid 112 from 
               
               
                 3rd day 
                 flow rate 
                 fluid output manifold 145 to fluid 
               
               
                   
                   
                 destination 140 (from second 
               
               
                   
                   
                 bioreactor 105) 
               
               
                 12:00 am 
                 Close second valve 127 on gas input 
                 Shuts off gas 122 to second 
               
               
                 3rd day 
                 manifold 125 
                 bioreactor 105 
               
               
                 12:20 am 
                 Deactivate pump 148 
                 Second bioreactor 105 is empty 
               
               
                 3rd day 
               
               
                   
               
             
          
         
       
     
         [0141]      FIG. 19  shows a diagram of a method  360  of distributing bioreactor chambers  105  according to a preferred embodiment of the present invention. Preferably, bioreactor system  100  comprises method  360 , as shown. Preferably, method  360  comprises the steps of: analyzing (step  362 ) at least one soil for at least one user; developing (step  364 ) at least one bioremediation prescription  365  for such at least one analyzed soil; preloading (step  366 ) at least one bioreactor chamber  105  with dry, inert microbes  210  according to such bioremediation prescription  365 ; and providing (step  368 ) such preloaded bioreactor chamber  105  to such user, as shown. 
         [0142]    Preferably, method  330  further comprises the step of maintaining (step  370 ) at least one bioreactor  102  on such user&#39;s site, as shown. 
         [0143]    Preferably, method  330  further comprises the step of remotely monitoring (step  372 ) such bioreactor  102 , as shown. 
         [0144]    Preferably, method  330  further comprises the step of replacing the at least one bioreaction chamber that was uninstalled (step  352 ) and disposed (step  354 ) by installing (step  374 ) such at least one preloaded bioreactor chamber  105  in at least one bioreactor  102  on such at least one user&#39;s site, as shown. 
         [0145]    Preferably, method  330  further comprises the step of installing (step  376 ) such preloaded bioreactor chamber  105  in at least one bioreactor  102  on such user&#39;s site according to at least one maintenance schedule  377 , as shown. 
         [0146]    Preferably, method  330  further comprises the step of applying (step  378 ) the bioreaction products (i.e. live microbes  101 ) of such preloaded bioreactor chamber  105  to such analyzed soil, as shown. 
         [0147]    Preferably, method  330  further comprises the step of re-analyzing (step  380 ) such analyzed soil to determine the effects of applying such bioreaction products (i.e. live microbes  101 ), as shown. 
         [0148]    Preferably, bioremediation prescription  365  comprises a bioremediation plan adapted to improve soil quality, to improve plant health, and to control pathogens, including such aspects as, for example: type and frequency of microbial additions to the irrigation water; type and frequency of fertilizer use; type and frequency of fungicide use; type and frequency of pesticide use; use of chitin to stimulate plant immune system responses to fungi; use of cover crops; use of crop rotation; sound stimulation of the plants; seed coating and pretreatments; etc. Typically, a bioremediation plan is developed by one skilled in the art of bioremediation, organic farming, farming, microbiology, plant biology, etc, as shown. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other bioremediation prescription aspects, such as amendments to change the soil structure, amendments to change the soil pH, soil sterilization by oxidation, addition of micronutrients, soil fumigation, biofumigation by green manures, bioaccumulation of radioactive particles, etc., may suffice. 
         [0149]    Preferably, maintenance schedule  377  comprises the schedule on which the user (preferably a bioreactor maintenance contractor) or the end-user (preferably a farmer or turf manager) replaces used bioreactor chambers  105  (and preferably sterilizes housing  250 ). 
         [0150]    Preferably, bioreactor chambers  105  are replaced after all of the bioreactor chambers  105  in all of the installed housings  250  are used. This schedule will depend on such factors as, for example, the number of housings  250  in use; the number of bioreactor chambers  105  in each housing  250 ; the bioreaction time for the species of microbe being used; the frequency of using bioreaction chambers  105 ; the speed of emptying live microbes  101  from bioreaction chambers  105  into the irrigation system  285 ; etc. Typically, maintenance schedule  377  will require bioreactor chambers  105  to be replaced about weekly. 
         [0151]      FIG. 20  shows a diagram of a method  390  of farming using bioreactor chambers  105  according to a preferred embodiment of the present invention. Preferably, bioreactor system  100  comprises method  390 , as shown. Preferably, method  390  comprises the steps of: analyzing (step  392 ) at least one soil for at least one user; analyzing (step  394 ) at least one chemical treatment history of such soil; developing (step  396 ) at least one bioremediation prescription  365  for such analyzed soil; providing (step  398 ) at least one on-site bioreactor  102 ; providing (step  400 ) at least one preloaded bioreactor chamber  105  containing inert microbes  210 , according to such bioremediation prescription  365 , adapted to be used with such on-site bioreactor  102 ; programming (step  402 ) such on-site bioreactor  102  to incubate such microbes  210  in such preloaded bioreactor chamber  105 ; and programming (step  404 ) such on-site bioreactor  102  to dispense such incubated microbes  210  (i.e. live microbes  101 ), as shown. Programming controller  150  to open and close solenoid valves in bioreactor  102  on a timed schedule can be done by one of ordinary skill in the art. 
         [0152]    Preferably, method  390  further comprises the step of developing (step  406 ) at least one bioremediation prescription  365  for such at least one analyzed soil, adapted to increase the functionality of crops grown in such analyzed soil, as shown. Functional crops are crops having proven enhanced nutrient content providing proven health benefits, as is known in the art of agriculture. For example, an onion grown in specially bacterially treated soil to have an enhanced calcium content, where calcium is known to help prevent bone loss in women, is a functional food created by applying bioremediation techniques to the soil. 
         [0153]    Preferably, method  390  further comprises the step of developing (step  408 ) at least one bioremediation prescription  365  for such analyzed soil, adapted to modify the growth of at least one cover crop grown in such at least one analyzed soil, wherein the modification of the growth of such cover crop has at least one beneficial effect on the growth of at least one cash crop grown in such analyzed soil after such cover crop, as shown. Cover crops are crops grown on soil, usually in the off-season, to preserve and improve the soil, as is known in the art of agriculture. Cover crops are typically turned under the soil to act as a green manure, prior to the cash crop being sown. For example, treating a cover crop of clover with nitrogen-fixing bacteria will result in enhanced growth of the cash crop planted afterward due to nitrogen-enrichment of the soil. 
         [0154]    Preferably, method  390  further comprises the step of developing (step  410 ) at least one bioremediation prescription  365  for such at least one analyzed soil, adapted to benefit bodies of water adjacent such analyzed soil, as shown. For example, a microbe may be selected that, in addition to suppressing pathogens in soil, will suppress pathogens in lakes and ponds which receive run-off from the treated soil. 
         [0155]    Preferably, method  390  further comprises the step of developing (step  412 ) at least one bioremediation prescription  365  for such at least one analyzed soil, adapted to decrease the concentration of toxins of crops grown in such analyzed soil, as shown. For example, mycotoxins and aflatoxins are types of harmful (poisonous and carcinogenic) fungal metabolites that some fungi generate in response to stress, which contaminate food crops hosting the fungi. Mycotoxin concentrations in crops may increase sharply when chemical fungicides are applied to the crops. However, inoculating crops with certain bacteria (such as, for example,  Bacillus pumilus ) inhibits mycotoxin production by fungi, decreasing the concentration of mycotoxin contaminating the food crop. 
         [0156]      FIG. 21  shows a diagram of additional interfaces to controller  150  beyond the ones previously described. Preferably, bioreactor system  100  further comprises at least one of monitor  420 , sensors  422 , weather monitor  428 , programming interface  424 , and prescription software  426 , which preferably interface with controller  150 . Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other controller interfaces, such as light controls, other temperature controls, irrigation system controls, alarms, etc., may suffice. 
         [0157]    Preferably, monitor  420  comprises a remote monitoring computer system which receives data at least about the status of bioreactor  102 , such as, for example, the number of bioreactor chambers  105  remaining, error warnings, housing  250  temperature, gas  122  pressure, sensor data, etc. Preferably, monitor  420  is remote from bioreactor  102 , preferably at the user&#39;s office, more preferably at the bioreactor maintenance contractor&#39;s location. Preferably, monitor  420  may be used to remotely control and/or program at least bioreactor  102 . Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other monitor arrangements, such as other data inputs, other data outputs, etc., may suffice. 
         [0158]    Preferably, sensors  422  comprise sensors at the end-user&#39;s site, such as, for example, soil moisture sensors, soil temperature sensors, air temperature sensors, weather monitor  428 , etc. Preferably, weather monitor  428  monitors and records weather conditions as is known in the art of weather stations. Preferably, the data from sensors  422  is transmitted to at least one of controller  150 , prescription software  426 , and/or monitor  420 . Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other sensors, such as soil pH, soil nitrogen content, soil oxygen content, solar intensity, etc., may suffice. 
         [0159]    Preferably, at least one of monitor  420 , sensors  422 , and weather monitor  428  are wirelessly connected to controller  150 , by means such as, for example, radio, wireless internet connection, cellular phone technology, etc. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other data connections, such as satellite uplinks, data cables, optical data transmission, etc., may suffice. 
         [0160]    Preferably, programming interface  424  comprises a user interface with controller  150  to permit monitoring, controlling, and/or programming controller  150 . Preferably, programming interface  424  comprises means such as, for example, a computer integral with housing  250 , a separate computer with a data cable connected to controller  150 , a monitor and keyboard connected directly to programming interface  424 , a wireless computer connection to controller  150 , etc. 
         [0161]    Preferably, prescription software  426  comprises software adapted to receive input about soil conditions, crop types, weather conditions, etc, and to provide a bioremediation prescription  365  in response to the provided data. Preferably, prescription software  426  is stored in programming interface  424 . Preferably, prescription software  426  is adapted to use data from sensors  422 , weather monitor  428 , monitor  420 , programming interface  424 , the user, the end-user, etc. Upon reading the teachings of this specification, those with ordinary skill in the art will now understand that, under appropriate circumstances, considering such issues as advances in technology, user preference, etc., other prescription software locations, such as at the remote monitoring computer, on the internet, on a home computer, on another type of computer processor, etc., may suffice. 
         [0162]    The preceding merely illustrates the principles of the invention. It will thus be appreciated that those skilled in the art will be able to devise various arrangements which, although not explicitly described or shown herein, embody the principles of the invention and are included within its spirit and scope. Furthermore, all examples and conditional language recited herein are principally intended expressly to be only for pedagogical purposes and to aid the reader in understanding the principles of the invention and the concepts contributed by the inventors to furthering the art, and are to be construed as being without limitation to such specifically recited examples and conditions. Moreover, all statements herein reciting principles, aspects, and embodiments of the invention, as well as specific examples thereof, are intended to encompass both structural and functional equivalents thereof. Additionally, it is intended that such equivalents include both currently known equivalents and equivalents developed in the future, i.e., any elements developed that perform the same function, regardless of structure. 
         [0163]    This description of the exemplary embodiments is intended to be read in connection with the figures of the accompanying drawing, which are to be considered part of the entire written description. In the description, relative terms such as “lower,” “upper,” “horizontal,” “vertical,” “above,” “below,” “up,” “down,” “top” and “bottom” as well as derivatives thereof (e.g., “horizontally,” “downwardly,” “upwardly,” etc.) should be construed to refer to the orientation as then described or as shown in the drawing under discussion. These relative terms are for convenience of description and do not require that the apparatus be constructed or operated in a particular orientation. Terms concerning attachments, coupling and the like, such as “connected” and “interconnected,” refer to a relationship wherein structures are secured or attached to one another either directly or indirectly through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise. 
         [0164]    Although the invention has been described in terms of exemplary embodiments, it is not limited thereto. Rather, the appended claims should be construed broadly, to include other variants and embodiments of the invention, which may be made by those skilled in the art without departing from the scope and range of equivalents of the invention. 
       Embodiments 
       [0165]    Live microbes  101  at least embody herein wherein such at least one microbe comprises at least one bacteria. 
         [0166]    Bioreactor chamber  105  at least embodies herein at least one bioreactor container adapted to contain at least one bioreaction; and at least embodies herein wherein such at least one microbe is stored within such at least one bioreactor container; and at least embodies herein wherein such at least one nutrient is stored within such at least one bioreactor container; and at least embodies herein wherein such at least one enzyme is stored within such at least one bioreactor container; and at least embodies herein wherein such at least one bioreactor container comprises at least one flexible container; and at least embodies herein wherein at least one interior of such at least one bioreactor container, containing such at least one microbe, is sterile of pathogens; and at least embodies herein at least one flexible container adapted to flexibly contain at least one fluid containing at least one cell culture; and at least embodies herein wherein such at least one flexible container has a volume of about 30 gallons; and at least embodies herein wherein such at least one flexible container is opaque; and at least embodies herein wherein about 400 acres of crops are treated per 30 gallon flexible container; and at least embodies herein bioreactor container means for containing at least one bioreaction; and at least embodies herein wherein such microbe means is stored within such bioreactor container means; and at least embodies herein wherein such bioreactor container means comprises at least one flexible container; and at least embodies herein flexible container means for flexibly containing at least one fluid containing at least one cell culture. 
         [0167]    Fluid source  110  at least embodies herein at least one fluid source adapted to provide at least one source of at least one fluid. 
         [0168]    Fluid  112  at least embodies herein wherein such at least one fluid comprises temperature-controlled water. 
         [0169]    Sterilized water  114  at least embodies herein such at least one fluid, and wherein such at least one fluid comprises sterilized water. 
         [0170]    Fluid input manifold  115  at least embodies herein at least one fluid input manifold adapted to provide at least one fluid to such at least one fluid input. 
         [0171]    Gas source  120  at least embodies herein at least one gas source adapted to provide at least one source of gas to such at least one gas input manifold. 
         [0172]    Gas input manifold  125  at least embodies herein one gas input manifold adapted to provide gas to such at least one gas input. 
         [0173]    Gas output manifold  135  at least embodies herein at least one gas output manifold adapted to receive gas from such at least one gas output. 
         [0174]    Fluid destination  140  at least embodies herein at least one irrigation system input adapted to input such fluid output from such at least one flexible container into such at least one irrigation system. 
         [0175]    Fluid output manifold  145  at least embodies herein at least one fluid output manifold adapted to receive fluid from such at least one fluid output. 
         [0176]    Controller  150  at least embodies herein at least one controller adapted to control the contents of such at least one flexible container; and at least embodies herein wherein such at least one controller controls the flow of fluid from such at least one fluid source to such at least one fluid input manifold; and at least embodies herein wherein such at least one controller controls the flow of fluid from such at least one fluid input manifold to such at least one fluid input; and at least embodies herein wherein such at least one controller controls the flow of fluid from such at least one fluid output to such at least one fluid output manifold; and at least embodies herein wherein such at least one controller controls the flow of fluid from such at least one fluid output manifold to at least one fluid destination. In this manner, by controlling fluid flow in and out of the at least one flexible container, controller  150  effectively selects which of the at least one flexible container(s) are utilized. Controller  150  also at least embodies herein wherein such at least one controller controls the flow of gas from such at least one gas source to such at least one gas input manifold; and at least embodies herein wherein such at least one controller controls the flow of gas from such at least one gas input manifold to such at least one gas input; and at least embodies herein wherein such at least one controller controls the flow of gas from such at least one gas output to such at least one gas output manifold; and at least embodies herein wherein such at least one controller controls the flow of gas from such at least one gas output manifold to at least one gas destination; and at least embodies herein wherein such at least one controller comprises at least one programmable irrigation timer; and at least embodies herein controller means for controlling the contents of such flexible container means. 
         [0177]    Fluid input  170  at least embodies herein at least one fluid input adapted to input fluid into such at least one flexible container; and at least embodies herein fluid input means for inputting fluid into such flexible container means. 
         [0178]    Filter  175  at least embodies herein wherein such at least one fluid input comprises at least one filter. 
         [0179]    One-way valve  176  at least embodies herein wherein such at least one fluid input comprises at least one one-way valve. 
         [0180]    Gas input  180  at least embodies herein at least one gas input adapted to input at least one gas into such at least one flexible container; and at least embodies herein wherein such at least one gas input is adapted to create bubbles in such at least one fluid; and at least embodies herein gas input means for inputting at least one gas into such flexible container means, wherein such gas input means is adapted to create bubbles in such at least one aqueous solution. 
         [0181]    Filter  185  at least embodies herein wherein such at least one gas input comprises at least one filter. 
         [0182]    One-way valve  186  at least embodies herein wherein such at least one gas input comprises at least one one-way valve. 
         [0183]    Aerator  187  at least embodies herein wherein such at least one gas input comprises at least one aerator. 
         [0184]    Gas output  190  at least embodies herein at least one gas output adapted to output at least one gas from such at least one flexible container; and at least embodies herein gas output means for outputting at least one gas from such flexible container means. 
         [0185]    Filter  195  at least embodies herein wherein such at least one gas output comprises at least one filter. 
         [0186]    One-way valve  196  at least embodies herein wherein such at least one gas output comprises at least one one-way valve. 
         [0187]    Fluid output  200  at least embodies herein at least one fluid output adapted to output fluid from such at least one flexible container; and at least embodies herein fluid output means for outputting fluid from such flexible container means. 
         [0188]    One-way valve  206  at least embodies herein wherein such at least one fluid output comprises at least one one-way valve. 
         [0189]    Inert microbes  210  at least embody herein at least one microbe adapted to provide at least one living microbe, wherein such at least one microbe may be in an inert, dry state; and at least embodies herein microbe means for providing at least one living microbe, wherein such microbe means is in an inert state. Inert microbes  210  may alternatively be in other states, such as wet. 
         [0190]    Dry, inert nutrient  215  at least embodies herein at least one nutrient adapted to provide at least one nutrient adapted to support the life of such at least one at least one microbe, wherein such at least one at least one nutrient is in an inert, dry state. 
         [0191]    Dry, inert enzymes  220  at least embody herein at least one enzyme adapted to provide at least one enzyme adapted to support the life of such at least one at least one microbe, wherein such at least one at least one enzyme is in an inert, dry state. 
         [0192]    Water-soluble capsules  225  at least embody herein at least one first water-soluble container adapted to contain such at least one microbe in at least one water-soluble container; and at least embodies herein at least one second water-soluble container adapted to contain such at least one nutrient in at least one water-soluble container; and at least embodies herein at least one third water-soluble container adapted to contain such at least one enzyme in at least one water-soluble container. 
         [0193]    Containers  230  at least embody herein at least one rigid stackable container adapted to provide at least one rigid stackable container adapted to hold such at least one flexible container. 
         [0194]    Housing  250  at least embodies herein at least one enclosure adapted to enclose at least such at least one flexible container; and at least embodies herein wherein such at least one enclosure maintains such at least one flexible container at least one selected temperature; and at least embodies herein wherein such at least one enclosure comprises at least one fluid input manifold adapted to provide fluid to such at least one fluid input; and at least embodies herein wherein such at least one enclosure comprises at least one fluid output manifold adapted to receive fluid from such at least one fluid output; and at least embodies herein wherein such at least one enclosure comprises at least one gas input manifold adapted to provide gas to such at least one gas input; and at least embodies herein wherein such at least one enclosure comprises at least one gas output manifold adapted to receive gas from such at least one gas output; and at least embodies herein wherein such at least one enclosure comprises at least one controller adapted to control the contents of such at least one flexible container; and at least embodies herein at least one enclosure adapted to enclose such at least two bioreactor chambers; and at least embodies herein wherein such at least one enclosure is thermally insulated; and at least embodies herein wherein such at least one enclosure comprises at least one temperature control adapted to control at least one temperature within such at least one enclosure; and at least embodies herein wherein such at least one enclosure comprises at least one fluid input manifold adapted to provide at least one fluid to each of such at least two bioreactor chambers; and at least embodies herein wherein such at least one enclosure comprises at least one fluid output manifold adapted to receive fluid from each of such at least two bioreactor chambers; and at least embodies herein wherein such at least one enclosure comprises at least one gas input manifold adapted to provide gas to each of such at least two bioreactor chambers; and at least embodies herein wherein such at least one enclosure comprises at least one gas output manifold adapted to receive gas from each of such at least two bioreactor chambers; and at least embodies herein wherein such at least one enclosure comprises at least one controller adapted to control such at least one temperature control, such at least one fluid input manifold, such at least one fluid output manifold, such at least one gas input manifold, and such at least one gas output manifold; and at least embodies herein enclosure means for enclosing at least such flexible container means; and at least embodies herein enclosure means for enclosing such at least two bioreactor chambers; and at least embodies herein wherein such enclosure means is thermally insulated; and at least embodies herein wherein such enclosure means comprises temperature control means for controlling at least one temperature within such enclosure means; and at least embodies herein wherein such enclosure means comprises fluid input manifold means for providing at least one fluid to each of such at least two bioreactor chambers; and at least embodies herein wherein such enclosure means comprises fluid output manifold means for receiving fluid from each of such at least two bioreactor chambers; and at least embodies herein wherein such enclosure means comprises gas input manifold means for providing gas to each of such at least two bioreactor chambers; and at least embodies herein wherein such enclosure means comprises gas output manifold means for receiving gas from each of such at least two bioreactor chambers; and at least embodies herein wherein such enclosure means comprises controller means for controlling such temperature control means, such fluid input manifold means, such fluid output manifold means, such gas input manifold means, and such gas output manifold means. 
         [0195]    Reverse osmosis  281  at least embodies herein wherein such sterilized water is sterilized by reverse osmosis treatment. 
         [0196]    Ultraviolet light system  283  at least embodies herein wherein such sterilized water is sterilized with ultraviolet radiation treatment. 
         [0197]    Irrigation system  285  at least embodies herein at least one irrigation system adapted to irrigating at least one crop; and at least embodies herein irrigation system means for irrigating at least one crop. 
         [0198]    Selecting  302  at least one inert microbe  210  at least embodies herein the step of selecting at least one inert microbe. 
         [0199]    Selecting  304  at least one dry, inert nutrient  215  at least embodies herein the step of selecting at least one dry, inert nutrient adapted to support the life of such at least one inert microbe. 
         [0200]    Placing  306  microbe  210  and nutrient  215  into bioreactor chamber  105  at least embodies herein the step of placing such at least one dry, inert microbe and such at least one dry, inert nutrient into at least one sterile bioreactor chamber. 
         [0201]    Storing  308  at least embodies herein the step of storing such at least one sterile bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient. 
         [0202]    Shipping  310  at least embodies herein the step of shipping such at least one sterile bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient to at least one user. 
         [0203]    Adding  312  water at least embodies herein the step of adding water to such at least one sterile bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient. 
         [0204]    Permitting  314  at least one bioreaction to occur at least embodies herein the step of permitting at least one bioreaction to occur. 
         [0205]    Placing  316  at least embodies herein the step of placing such at least one dry, inert microbe into at least one water-soluble container prior to placing such at least one dry, inert microbe into such at least one sterile bioreactor chamber. 
         [0206]    Placing  318  at least embodies herein the step of placing such at least one dry, inert nutrient into at least one water-soluble container prior to placing such at least one dry, inert nutrient into such at least one sterile bioreactor chamber. 
         [0207]    Selecting  320  at least one dry, inert enzyme at least embodies herein the step of selecting at least one dry, inert enzyme adapted to support the life of such at least one dry, inert microbe. 
         [0208]    Placing  322  such at least one dry, inert enzyme at least embodies herein the step of placing such at least one dry, inert enzyme into such at least one sterile bioreactor chamber. 
         [0209]    Placing  324  at least embodies herein the step of placing such at least one dry, inert enzyme into at least one water-soluble container prior to placing such at least one dry, inert enzyme into such at least one sterile bioreactor chamber. 
         [0210]    Selecting  332  at least embodies herein the step of selecting at least one dry, inert microbe. 
         [0211]    Selecting  334  at least embodies herein the step of selecting at least one dry, inert nutrient adapted to support the life of such at least one dry, inert microbe. 
         [0212]    Selecting  336  at least embodies herein the step of selecting at least one previously sterilized bioreactor chamber. 
         [0213]    Receiving  338  at least embodies herein the step of receiving such at least one bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient from at least one manufacturer. 
         [0214]    Installing  340  at least embodies herein the step of installing such at least one bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient in at least one bioreactor. 
         [0215]    Adding fluid  342  at least embodies herein the step of adding fluid to such at least one bioreactor chamber containing such at least one dry, inert microbe and such at least one dry, inert nutrient. 
         [0216]    Adding oxygen  344  at least embodies herein the step of adding oxygen to such at least one bioreactor chamber, after such step of adding fluid to such at least one bioreactor chamber. 
         [0217]    Waiting  346  at least embodies herein the step of waiting for at least one bioreaction in such at least one bioreactor chamber to generate a useful number of live, active microbes. 
         [0218]    Harvesting  348  at least embodies herein the step of harvesting such useful number of live, active microbes. 
         [0219]    Adding  350  at least embodies herein the step of wherein the step of such step of harvesting such useful number of live, active microbe comprises the step of adding such useful number of live, active microbes into at least one irrigation system. 
         [0220]    Uninstalling  352  at least embodies herein the step of uninstalling such at least one bioreactor chamber. 
         [0221]    Disposing  354  at least embodies herein the step of disposing of such at least one bioreactor chamber. 
         [0222]    Analyzing  362  at least embodies herein the step of analyzing at least one soil for at least one user. 
         [0223]    Developing  364  at least embodies herein the step of developing at least one bioremediation prescription for such at least one analyzed soil. 
         [0224]    Preloading  366  at least embodies herein the step of loading at least one bioreactor chamber with dry, inert microbes according to such at least one bioremediation prescription. 
         [0225]    Providing  368  at least embodies herein the step of providing such at least one loaded bioreactor chamber to such at least one user. 
         [0226]    Maintaining  370  at least embodies herein the step of maintaining at least one bioreactor on such at least one user&#39;s site. 
         [0227]    Remotely monitoring  372  at least embodies herein the step of remotely monitoring such at least one bioreactor. 
         [0228]    Installing  374  at least embodies herein the step of installing such at least one loaded bioreactor chamber in at least one bioreactor on such at least one user&#39;s site. 
         [0229]    Installing  376  at least embodies herein the step of installing such at least one loaded bioreactor chamber in at least one bioreactor on such at least one user&#39;s site according to at least one maintenance schedule. 
         [0230]    Applying  378  at least embodies herein the step of applying the bioreaction products of such at least one preloaded bioreactor chamber to such at least one analyzed soil. 
         [0231]    Re-analyzing  380  at least embodies herein the step of re-analyzing such at least one analyzed soil to determine the effects of applying such bioreaction products. 
         [0232]    Analyzing  392  at least embodies herein the step of analyzing at least one soil for at least one user. 
         [0233]    Analyzing  394  at least embodies herein the step of analyzing at least one chemical treatment history of such at least one soil. 
         [0234]    Developing  396  at least embodies herein the step of developing at least one bioremediation prescription for such at least one analyzed soil. 
         [0235]    Providing  398  at least embodies herein the step of providing at least one on-site bioreactor wherein the step of such at least one on-site bioreactor comprises at least one programmable controller. 
         [0236]    Providing  400  at least embodies herein the step of providing at least one loaded bioreactor chamber containing inert microbes, according to such at least one bioremediation prescription, adapted to be used with such on-site bioreactor. 
         [0237]    Programming  402  at least embodies herein the step of programming such at least one on-site bioreactor to incubate such microbes in such at least one loaded bioreactor chamber. 
         [0238]    Programming  404  at least embodies herein the step of programming such at least one on-site bioreactor to dispense such incubated microbes. 
         [0239]    Developing  406  at least embodies herein the step of wherein the step of such step of developing at least one bioremediation prescription for such at least one analyzed soil is adapted to increase the functionality of crops grown in such at least one analyzed soil. 
         [0240]    Developing  408  at least embodies herein the step of wherein the step of such step of developing at least one bioremediation prescription for such at least one analyzed soil is adapted to modify the growth of at least one cover crop grown in such at least one analyzed soil, wherein the step of the modification of the growth of such at least one cover crop has at least one beneficial effect on the growth of at least one cash crop grown in such at least one analyzed soil after such at least one cover crop. 
         [0241]    Developing  410  at least embodies herein the step of wherein the step of such step of developing at least one bioremediation prescription for such at least one analyzed soil is adapted to benefit bodies of water adjacent such at least one analyzed soil. 
         [0242]    Developing  412  at least embodies herein the step of wherein the step of such step of developing at least one bioremediation prescription for such at least one analyzed soil is adapted to decrease the concentration of toxins of crops grown in such at least one analyzed soil. 
         [0243]    Although applicant has described applicant&#39;s preferred embodiments of this invention, it will be understood that the broadest scope of this invention includes modifications such as diverse shapes, sizes, and materials. Such scope is limited only by the below claims as read in connection with the above specification. 
         [0244]    Further, many other advantages of applicant&#39;s invention will be apparent to those skilled in the art from the above descriptions and the below claims.

Technology Category: 8