Source: https://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000634
Timestamp: 2019-04-24 01:57:44+00:00

Document:
Three strains (DMKU-RE28, DMKU-RE43T and DMKU-RE123) of a novel anamorphic yeast species were isolated from rice leaf tissue collected in Thailand. DNA sequence analysis demonstrated that the species forms a sister pair with Candida ranongensis CBS 10861T but differs by 24–30 substitutions in the LSU rRNA gene D1/D2 domains and 30–35 substitutions in the ITS region. A phylogenetic analysis based on both the small and the large rRNA gene subunits confirmed this connection and demonstrated the presence of a clade that also includes Candida catenulata, Candida mesorugosa, Candida neorugosa, Candida pseudorugosa, Candida rugosa and Candida scorzettiae. The clade is not closely affiliated to any known teleomorphic genus, and forms a well-separated lineage from currently recognized genera of the Saccharomycetales. Hence, the genus Diutina gen. nov. is proposed to accommodate members of the clade, including Diutina siamensis f.a. sp. nov. and the preceding seven Candida species. The type strain is DMKU-RE43T ( = CBS 13388T = BCC 61183T = NBRC 109695T).
The GenBank/EMBL/DDBJ accession numbers of the SSU and the LSU rRNA gene sequences of Diutina siamensis f.a. DMKU-RE43T Diutina mesorugosa CBS 12656T Diutina rugosa CBS 613T Diutina pseudorugosa CBS 10433T Diutina neorugosa CBS 12627T Diutina ranongensis CBS 10861T Diutina catenulata CBS 565T and Diutina scorzettiae CBS 10107T are KT336715–KT336722, respectively. The MycoBank numbers of Diutina rugosa comb. nov., Diutina catenulata comb. nov., Diutina mesorugosa comb. nov., Diutina neorugosa comb. nov., Diutina pseudorugosa comb. nov., Diutina ranongensis comb. nov., Diutina scorzettiae comb. nov. and Diutina siamensis f.a., sp. nov. are MB 813768, MB 813778, MB 813786, MB 813787, MB 813788, MB 813874, MB 813789 and MB 813790, respectively.
A supplementary figure is available with the online Supplementary Material.
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