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'Shine Muscat' is a diploid table grape cultivar which is a hybrid between Akitsu-21 and Hakunan. It was registered as No.13,891 under the Seed and Seedlings Law of Japan in 2006. It has large yellow-green berries, crisp flesh texture, muscat flavor, high soluble solids concentration and low acidity, which make them to be popular in Taiwan. However, the plant displayed transplanting leaf deformation, leaf curl and chlorotic mottle during cultivating in field which may be due to virus infection. Thus, the objectives of this study were utilized plant tissue culture to establish plantlet in vitro which offer thermotherapy combined with shoot tip culture to eliminate of virus-infected plant. After treatment, the specific virus-free health seedling can be derived, exploited for rapid multiplication and produced health seedling of this plant cultivar. Furthermore, the use of chemical forcing without scarification methods to reaserch in best commercial production of 'Shine Muscat' Grape. The axillary bud explants took from greenhouse and the field were sterilized in 1% NaOCl for 7、8、9、10 and 15, cultured on medium duration 30 days, the survival percentage was the highest, and the contamination percentage was decreased of the explants in greenhouse were sterilized for 10 min. The survival and contamination percentage were showed better of the explants in the field were sterilized for 10 or 15 min. In addition, the browning number was the most of the explants in greenhouse were sterilized for 15 min. Dissected new shoots were cultured on the half strength of MS medium contains 0、0.1、1、0.2、2 mg/L IBA, supplemented with 2 mg/L IBA were getting better of rooting percentage、root number and root length on the 30th day after culture. Instead of supplemented with 0.1 mg/L IBA were getting decreased of rooting percentage. A high survival rate of in vitro-shoot tip of 90% was achieved by thermotherapy at 37°C for 30、35、40 and 45 days, regeneration efficacy and survival rate of subculture every four weeks were decreased of the explants were treatment for 30 days.It is presumed that the explants were subcultured only one time before treatment, have not recover its enough juvenility yet, and caused browning degree of shoot tip much severity after heat stress. The result of 100% GLRaV-3 virus elimination of plantlet in vitro after different treatment day. The use of 2-chloroethanol、hydrogen cyanamide、distilled water without scarification methods to carry out bud forcing, the budbreak was the highest, budbreak uniformity, and the shoot length did not very significantly by 2-chloroethanol. Besides, the time of budbreak was the earliest of hydrogen cyanamide, but the budbreak was lower than 2-chloroethanol treatment. It is presumed that hydrogen cyanamide was able to activate Ca2+-ATPase, and calcium was played a role in signal transduction on release dormancy. Furthermore, the concentrations of calcium and boron on 'Shine Muscat' canes were less than 'Kyoho' and 'Honey Red', maybe 'Shine Muscat' is easy cultivar to absence of calcium. The concentrations of manganese and calcium/boron ratio on 'Shine Muscat' leaves without inflorescence were significantly higher than with inflorescence. It is presumed that there was a nutrient-competed relationship between the development of inflorescence and the growth of leaf in new shoot, no time enough be carried out to distribute or transport, and caused 'Shine Muscat' plant displayed leaf deformation、leaf curl and chlorotic mottle during growing season.

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