Source: http://www.conscientiabeam.com/archive/97/03-2015/1
Timestamp: 2019-04-25 17:43:26+00:00

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L. L. Veeck, "Oocyte assessment and biological performance," Ann. N Y Acad. Sci., vol. 541, pp. 259-274, 1988.
P. A. Almeida and V. N. Bolton, "The relationship between chromosomal abnormalities in the human oocyte and fertilization in vitro," Hum. Reprod., vol. 9, pp. 343-346, 1994.
P. A. Almeida and V. N. Bolton, "Immaturity and chromosomal abnormalities in oocytes that fail to develop pronuclei following insemination in vitro," Hum. Reprod., vol. 3, pp. 8229-232, 1993.
R. R. Angell, J. Xian, and J. Keith, "Chromosome anomalies in human oocytes in relation to age," Hum. Reprod., vol. 8, pp. 1047-1054, 1993.
C. W. J. D. Kok, S. M. Looman, and E. R. Velde, "High number of oocytes obtained after ovarian hyperstimulation for in vitro fertilization or intracytoplasmic sperm injection is not associated with decreased pregnancy outcome," Fertil Steril, vol. 85, pp. 918–924, 2006.
L. Zhang, S. Jiang, P. J. Wozniak, X. Yang, and R. A. Godke, "Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro," Mol. Reprod Dev., vol. 40, pp. 338-344, 1995.
L. Gregory and H. J. Leese, "Determinants of oocyte and preimplantation embryo quality: Metabolic requirements and potential role of cumulus cells," J. Br. Fertil Soc. (Human Reprod Suppl), vol. 11, pp. 96-102, 1996.
T. Mori, T. Amano, and H. Shimizu, "Roles of gap junctional communication of cumulus cells in cytoplasmic maturation of porcine oocytes cultured in vitro," Biol. Reprod, vol. 62, pp. 913–919, 2000.
G. Ruvolo, L. Bosco, A. Pane, G. Morici, E. Cittadini, and M. C. Roccheri, "Lower apoptosis rate in human cumulus cells after administration of recombinant luteinizing hormone to women undergoing ovarian stimulation for in vitro fertilization procedures," Fertil Steril, vol. 87, pp. 542-546, 2007.
E. Warzych, E. Pers-Kamczyc, A. Krzywak, S. Dudzi?ska, and D. Lechniak, "Apoptotic index within cumulus cells is a questionable marker of meiotic competence of bovine oocytes matured in vitro," Reprod Biol., vol. 13, pp. 82-87, 2013.
Y. Q. Yuan, A. Van Soom, J. L. M. R. Leroy, J. Dewulf, A. Van Zeveren, A. De Kruif, and L. J. Peelman, "Apoptosis in cumulus cells, but not in oocytes, may influence bovine embryonic developmental competence," Theriogenology, vol. 63, pp. 2147–2163, 2005.
G. Ruvolo, R. R. Fattouh, L. Bosco, A. M. Brucculeri, and E. Cittadini, "New molecular markers for the evaluation of gamete quality," J. Assist. Reprod Genet., vol. 30, pp. 207-212, 2013.
M. Mattioli and B. Barboni, "Signal transduction mechanism for LH in the cumulus-oocyte complex," Mol. Cell Endocrinol, vol. 161, pp. 19-23, 2000.
V. N. Bolton, S. M. Hawes, C. T. Taylor, and J. H. Parsons, "Development of spare human preimplantation embryos in vitro: An analysis of the correlations among gross morphology, cleavage rates, and development to the blastocyst," J. In Vitro Fert. Embryo. Transf., vol. 6, pp. 30-35, 1989.
K. Nakahara, H. Saito, T. Saito, M. Ito, N. Ohta, T. Takahashi, and M. Hiroi, "The incidence of apoptotic bodies in membrana granulosa can predict prognosis of ova from patients participating in in vitro fertilization programs," Fertil Steril, vol. 68, pp. 312-317, 1997.
K. S. Lee, B. S. Joo, Y. J. Na, M. S. Yoon, O. H. Choi, and W. W. Kim, "Cumulus cells apoptosis as an indicator to predict the quality of oocytes and the outcome of IVF-ET," J. Assist Reprod Genet., vol. 18, pp. 490-498, 2001.
R. B. Staigmiller and R. M. Moor, "Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicle," Gamete Res., vol. 9, pp. 221–229, 1984.
M. A. El-Fouly, B. Cook, M. Nekola, and A. V. Nalbandov, "Role of the ovum in follicular luteinization," Endocrinology, vol. 87, pp. 286-293, 1970.
R. Li, R. J. Norman, D. T. Armstrong, and R. B. Gilchrist, "Oocyte-secreted factor(S) determine functional differences between bovine mural granulosa cells and cumulus cells," Biol Reprod, vol. 63, pp. 839-845, 2000.
L. Bosco, G. Ruvolo, G. Morici, M. Manno, E. Cittadini, and M. C. Roccheri, "Apoptosis in human unfertilized oocytes after intracytoplasmic sperm injection," Fertil Steril, vol. 84, pp. 1417-1423, 2005.
E. Høst, A. L. Mikkelsen, S. Lindenberg, and S. Smidt-Jensen, "Apoptosis in human cumulus cells in relation to maturation stage and cleavage of the corresponding oocyte," Acta Obstet Gynecol Scand, vol. 79, pp. 936-940, 2000.
O. Moffatt, S. Drury, M. Tomlinson, M. Afnan, and D. Sakkas, "The apoptotic profile of human cumulus cells changes with patient age and after exposure to sperm but not in relation to oocyte maturity," Fertil Steril, vol. 77, pp. 1006-1011, 2002.
E. Høst, A. Gabrielsen, S. Lindenberg, and S. Smidt-Jensen, "Apoptosis in human cumulus cells in relation to zona pellucida thickness variation, maturation stage, and cleavage of the corresponding oocyte after intracytoplasmic sperm injection," Fertil Steril, vol. 77, pp. 511-515, 2002.
M. Toya, H. Saito, N. O. T. Saito, T. Kaneko, and M. Hiroi, "Oocyte quality in patients with moderate and severe endometriosis," Fertil Steril, vol. 73, pp. 344-350, 1999.
P. Falcone, L. Gambera, M. Pisoni, V. Lofiego, V. De Leo, L. Mencaglia, and P. Piomboni, "Correlation between oocyte preincubation time and pregnancy rate after intracytoplasmic sperm injection," Gynecol Endocrinol, vol. 24, pp. 295-299, 2008.
Oocyte quality is one of the main factors for the success of in vitro fertilization protocols. Apoptosis is known to affect oocyte quality and may impair subsequent embryonic development and implantation. The aim of this study was to investigate the apoptosis rate of single and pooled cumulus cells of cumulus cell–oocyte complexes (COCs), as markers of oocyte quality, prior to intracytoplasmatic sperm injection (ICSI).We investigated the apoptosis rate by TUNEL assay (DNA fragmentation) and caspase-3 immunoassay of single and pooled cumulus cells of COCs. The results showed that DNA fragmentation in cumulus cells was remarkably lower in patients who achieved a pregnancy than in those who did not. Cumulus cell apoptosis rate could be a marker for the selection of the best oocytes to be fertilized by intracytoplasmatic sperm injection.
This study is one of very few studies which have investigated the apoptosis rate by TUNEL assay and caspase-3 immunoassay of single and pooled human cumulus cells of COCs. The apoptosis rate could be a marker for the selection of the best oocytes to be fertilized by intracytoplasmatic sperm injection.
Z. Bar-Joseph, Z. Siegfried, M. Brandeis, and B. Brors, "Genome-wide transcriptional analysis of the human cell cycle identifies genes differentially regulated in normal and cancer cells," in Proceedings of the National Academy of Sciences of the United States of America, 2008, pp. 955-960.
L. L. Breeden, "Periodic transcription: A cycle within a cycle," Curr. Biol., vol. 13, pp. 31-38, 2003.
R. J. Cho, M. Huang, M. J. Campbell, and H. Dong, "Transcriptional regulation and function during the human cell cycle," Nature Genetics, vol. 27, pp. 48-54, 2001.
A. Oliva, A. Rosebrock, F. Ferrezuelo, and S. Pyne, "The cell cycle-regulated genes of schizosaccharomyces pombe," PLoS Biol., vol. 3, p. 225, 2005.
D. A. Orlando, C. Y. Lin, A. Bernard, and J. Y. Wang, "Global control of cell-cycle transcription by coupled CDK and network oscillators," Nature, vol. 453, pp. 944-947, 2008.
X. Peng, R. K. Karuturi, L. D. Miller, and K. Lin, "Identification of cell cycle-regulated genes in fission yeast," Molecular Biology of the Cell, vol. 16, pp. 1026-1042, 2005.
G. Rustici, J. Mata, K. Kivinen, and P. Lio, "Periodic gene expression program of the fission yeast cell cycle," Nature Genetics, vol. 36, pp. 809-817, 2004.
P. T. Spellman, G. Sherlock, M. Q. Zhang, and V. R. Lyer, "Comprehensive identification of cell cycle-regulated genes of the yeast saccharomyces cerevisiae by microarray hybridization," Molecular Biology of the Cell, vol. 9, pp. 3273-3297, 1998.
S. Cooper, "A unifying model for the G1 period in prokaryotes and eukaryotes," Nature, vol. 280, pp. 17-19, 1979.
S. Cooper, "Cell cycle analysis and microarrays," Trends in Genetics, vol. 18, pp. 289-290, 2002.
S. Cooper, "On a heuristic point of view concerning the expression of numerous genes during the cell cycle," IUBMB Life, vol. 64, pp. 10-17, 2012.
S. Cooper, "The continuum model and c-myc synthesis during the division cycle," J. Theor. Biol., vol. 135, pp. 393-400, 1988.
S. Cooper, "The continuum model and G1-control of the mammalian cell cycle," Prog Cell Cycle Res., vol. 4, pp. 27-39, 2000.
S. Cooper, "The continuum model: Statistical implications," J. Theor. Biol., vol. 94, pp. 783-800, 1982.
K. Shedden and S. Cooper, "Analysis of cell-cycle-specific gene expression in human cells as determined by microarrays and double-thymidine block synchronization," Proc. Natl. Acad. Sci. USA, vol. 99, pp. 4379-4384, 2002.
K. Shedden and S. Cooper, "Analysis of cell-cycle-specific gene expression in saccharomyces cerevisiae as determined by microarrays and multiple synchronization methods," Nuc. Acids Res., vol. 30, pp. 2920-2929, 2002.
S. Cooper and K. Shedden, "Microarrays and the relationship of mRNA variation to protein variation during the cell cycle," J. Theor. Biol., vol. 249, pp. 574-581, 2007.
S. Cooper, K. Shedden, and D. Vu-Phan, "Invariant mRNA and mitotic protein breakdown solves the Russian doll problem of the cell cycle," Cell Biol. Int., vol. 33, pp. 10-18, 2009.
S. Cooper, "Is whole-culture synchronization biology's perpetual potion pachine?," Trends in Biotechnology, vol. 26, pp. 266-269, 2004.
S. Cooper, "Minimally disturbed, multi-cycle, and reproducible synchrony using a eukaryotic baby machine," Bioessays, vol. 24, pp. 499-501, 2002.
S. Cooper, "Reappraisal of G1-phase arrest and synchronization by lovastatin," Cell Biol. Int., vol. 26, pp. 715-727, 2002.
S. Cooper, "Reappraisal of serum starvation, the restriction point, G0, and G1-phase arrest points," FASEB J., vol. 17, pp. 333-340, 2003.
S. Cooper, "Rejoinder: Whole-culture synchronization cannot, and does not, synchronize cells," Trends in Biotechnology, vol. 22, pp. 274-276, 2004.
S. Cooper, "Rethinking synchronization of mammalian cells for cell-cycle analysis," Cell Mol Life Sci., vol. 6, pp. 1099-1106, 2003.
S. Cooper, "The schaechter-bentzon-maaløe experiment and the analysis of cell cycle events in eukaryotic cells," Trends in Microbiology, vol. 10, pp. 169-173, 2002.
S. Cooper, "Toward a standard system for the mammalian cell cycle," ASM News, vol. 66, pp. 71-75, 2000.
S. Cooper, K. Z. Chen, and S. Ravi, "Thymidine block does not synchronize L1210 mouse leukaemic cells: Implications for cell cycle control, cell cycle analysis and whole-culture synchronization," Cell Prolif., vol. 41, pp. 156-167, 2008.
S. Cooper and M. Gonzalez-Hernandez, "Experimental reconsideration of the utility of serum starvation as a method for synchronizing mammalian cells," Cell Biology International, vol. 33, pp. 71-77, 2009.
S. Cooper, G. Iyer, M. Tarquini, and P. Bissett, "Nocodazole does not synchronize cells: Implications for cell-cycle control and whole-culture synchronization," Cell Tissue Res., vol. 324, pp. 237-242, 2006.
S. Cooper, M. Paulsen, M. Ljungman, and D. Vu-Phan, "Membrane-elution analysis of content of cyclins A, B1, and E during the unperturbed mammalian cell cycle," BMC Cell Division, vol. 2, p. 28, 2007.
S. Cooper, "Schizosaccharomyces pombe grows exponentially during the division cycle with no rate change points," FEMS Yeast Research, vol. 13, pp. 650-658, 2013.
Normalization of raw data on gene expression during the cell cycle obscures the original experimental data and makes it appear as if all genes have the same numerical level of cyclical expression. Results that would not support gene expression because of minimal variations thus appear, after normalization, to be stronger than they actually are. Consideration of the effect of normalization raises critical questions about many experiments on the cell cycle dependent variation of gene expression—that is, proposed cyclical changes in mRNA content—during the cell cycle.
This paper demonstrates that normalization makes minimal variations appear much stronger than they are in the raw data, and thus one should be skeptical of some published results proposing cell-cycle specific gene expression.

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