Source: http://www.proceedings.blucher.com.br/article-details/influncia-do-ph-na-produo-de-lipase-em-melao-de-soja-por-geotrichum-candidum-11143
Timestamp: 2019-04-19 08:49:22+00:00

Document:
INFLUÊNCIA DO pH NA PRODUÇÃO DE LIPASE EM MELAÇO DE SOJA POR Geotrichum candidum.
Atualmente, as lipases constituem o grupo mais importante de enzimas com enorme potencial de aplicação na área biotecnológica, principalmente no processo de transesterificação enzimática para produção de um biodiesel mais puro e com pouca geração de resíduos, porém este é um processo caro devido aos altos custos na produção da lipase. Destacam-se as lipases de origem microbiana, pelas características próprias como a estabilidade a altas temperaturas, ampla faixa de atuação em relação ao pH, além da sua seletividade na resolução de misturas racêmicas. Entretanto, há limitações em sua aplicação industrial, como o custo de obtenção que é determinado pelo rendimento da produção, pelas condições do processo e pela estabilidade da enzima. Neste contexto, este trabalho tem como objetivo estudar a influência do pH na produção de lipase em meio contendo melaço de soja, por Geotrichum candidum, visando aumentar a produtividade do processo, diminuindo custos. Os testes foram realizado num tempo de 24 horas de fermentação submersa com concentração do melaço de soja, no meio, de 200 g/L numa temperatura de 30°C. A atividade de lipase foi determinada por titulação com NaOH 0,05N. A atividade lipolítica máxima alcançada foi de 11,4 U/mL em meio de pH 3.
 Açikel, Ü.; Ersan, M.; Açikel, Y. S.
 Bussamara, R.; Fuentefria, A. M.; Oliveira, E.
 P.; Schrank, A.; Vainstein, M. H.
 Isolation of a lipase secreting yeast for enzyme production in a pilot-plant scale batch fermentation. Bioresource Technology, v. 101, p. 268-275, 2010.
 Dalmau, E., Montesinos, J. L., Lotti, M., Andamp; Casas, C. - Effect of different carbon sources on lipase production by Candida rugosa. Enzyme and Microbial Technology, v. 26, p. 657–663, 2000.
 Del Rio, J. L., Serra, P., Valero, F., Poch, M., Andamp; Sula, C. - Reaction scheme of lipase production by C. rugosa growing on olive oil. Biotechnology Letters, v. 12, p. 835– 839, 1990.
 Ghaly, A.E.; Dave, D.; Brooks, M.S. Andamp; Budge, S. - Production of biodiesel by enzymatic transesterification: Review. American Journal of Biochemistry and Biotechnology, v. 6, p. 54-76, 2010.
 Hasan, F.; Shah, A. A.; Hameed, A. - Industrial applications of microbial lipases. Enzyme and Microbial Technology, v. 39, p. 235- 251, 2006.
 Kontkanen, H.; Tenkanen, M.; Fagerstrom, R.; Reinikainen, T. - Characterisation of steryl esterase activities in commercial lipase preparations. J. Biotechnol., v. 108, p.51- 59, 2004.
 Kumar, S.; Kikon, K.; Upadhyay, A.; Kanwar, S. S.; Gupta, R. Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expression and Purification, v. 41, p. 38-44, 2005.
 Li, C.; Cheng, C.; Cheng, T. Fed-batch production of lipase by Acinetobacter radioresistens using Tween80 as the carbon source. Biochemical Engineering Journal, v. 19, p. 25-31, 2004.
 Liu, C.; Chen, C.; Wang, Y.; Chang, J. Fermentation strategies for the production of lipase by an indigenous isolate Burkholderia sp. C Biochemical Engineering Journal, v. 58-59, p. 96-102, 2011.
 Liu, Z.; Chi, Z.; Wang, L.; Li, J. Production, purification and characterization of an extracellular lipase from Aureobasidium pulluans HN2-3 with potential application for the hydrolysis of edible oils. Biochemical Engineering Journal, v. 40, p. 445-451, 2008.
 Mladenoska, I.; Dimitrovski, A. – Lipase production by Geotrichum candidum – M2. Bulletin of the Chemists and Technologists of Macedonia, v. 20, p. 39- 43, 2001.
 Paques, F. W.; Macedo, G. A. Plant lipases from latex: properties and industrial applications. Química Nova, v. 29, n. 1, p. 93-99, 2006.
 Pinheiro, T. L. F.; Lipke, N. R.; Kempka, A. P.; Menoncin, S.; Oliveira, D.; Treichel, H.; Luccio, M.; Freire, D. M. G. - Response surface method to optimize the production and characterization of lipase from Penicillium verrucosum in solid state fermentation. Bioprocess and Biosystems Engineering, v. 31, n. 2, p. 119-125, 2008.
 Rajendran, A., Palanisamy, A., Andamp; Thangavelu, V. - Evaluation of medium components by Plackett-Burman statistical design for lipase production by Candida rugosa and kinetic modeling. Chinese Journal of Biotechnology, v. 34, p. 436–444, 2008.
 Rigo, E.; Ninow, J. L.; Luccio, M. D.; Oliveira, J. V.; Polloni, A. E.; Remonatto, D.; Arbter, F.; Vardanega, R.; Oliveira, D.; Treichel, H. Lipase production by solid fermentation of soybean meal with different supplements. LWT – Food Science and Technology, v. 43, p. 1132-1137, 2010.
 Romão, B. B. Ethanol production from acidic hydrolysed of soybean molasses. Master dissertation, Federal University of Uberlândia, 2011.
 Salihu, A.; Alam, M. Z.; AbdulKarim, M. I.; Salleh, H. M. Lipase production: An insight in the utilization of renewable agricultural risidues. Resources, Conservation and Recycling, v. 58, p. 36-44, 2012.
 Salihu, A.; Alam, M. Z.; AbdulKarim, M. I.; Salleh, H. M. Optimization of lipase production by Candida cylindracea in palm oil Mill effluent based médium using statistical experimental design. Journal of Molecular Catalysis B: Enzymatic, v. 69,p. 66-73, 2011.
 Silva, F. B. Ethanol production from enzymatically hydrolysed of soybean molasses. Master dissertation, Federal University of Uberlândia, 2011.
 Siqueira, P. F. Production of bio-ethanol from soybean molasses by Saccharomyces cerevisiae. Master Dissertation, Federal University of Paraná/ Universities of Provence and of the Mediterranean, 2007.
 Tan, T.; Zhang, M.; Wang, B.; Ying, C.; Deng, L. Screening of high lipase producing Candida sp. and production of lipase by fermentation. Process Biochemistry, v. 39, p. 459-465, 2003.
 Tommaso, G.; Moraes, B. S.; Macedo, G. C.; Silva, G. S.; Kamimura, E. S. Production of lipase from Candida rugosa using cheese whey through experimental design and surface response methodology. Food Bioprocess Technol, v. 4, n. 8, p. 1473- 1481, 2010.
 Treichel, H., de Oliveira, D., Mazutti, M. A., Di Luccio, M., Andamp; Oliveira, J. V. - A review on microbial lipases production. Food and Bioprocess Technology, v. 3, p. 182–196, 2010.
 Vargas, G. D. L. P.; Treichel, H.; Oliveira, D.; Beneti, S. C.; Freire, D. M. G.; Luccio, M. - Optimization of lipase production by Penicillium simplicissimum in soybean meal. Journal of Chemical Technology and Biotechnology, v. 83, n. 1, p. 47-54, 2007.
 Yang, X.; Wang, B.; Cui, F.; Tan, T. Production of lipase by repeated batch fermentation with immobilized Rhizopus arrhizus. Process Biochemistry, v. 40, n. 6, p. 2095–2103, 2005.
Takarada, C. S.; Júnior, W.G.M.; Resende, M.M.; "INFLUÊNCIA DO pH NA PRODUÇÃO DE LIPASE EM MELAÇO DE SOJA POR Geotrichum candidum.", p. 634-638 . In: . São Paulo: Blucher, 2014.
Açikel, Ü.; Ersan, M.; Açikel, Y. S. Optimization of critical medium components using reponse surface methodologyfor lipase production by Rhizopus delemar. Food and Bioproducts Processing, v. 88, p.31-39, 2010. Bussamara, R.; Fuentefria, A. M.; Oliveira, E. S.; Broetto, L.; Simcikova, M.; Valente, P.; Schrank, A.; Vainstein, M. H. Isolation of a lipase secreting yeast for enzyme production in a pilot-plant scale batch fermentation. Bioresource Technology, v. 101, p. 268-275, 2010. Dalmau, E., Montesinos, J. L., Lotti, M., Andamp; Casas, C. - Effect of different carbon sources on lipase production by Candida rugosa. Enzyme and Microbial Technology, v. 26, p. 657–663, 2000. Del Rio, J. L., Serra, P., Valero, F., Poch, M., Andamp; Sula, C. - Reaction scheme of lipase production by C. rugosa growing on olive oil. Biotechnology Letters, v. 12, p. 835– 839, 1990. Ghaly, A.E.; Dave, D.; Brooks, M.S. Andamp; Budge, S. - Production of biodiesel by enzymatic transesterification: Review. American Journal of Biochemistry and Biotechnology, v. 6, p. 54-76, 2010. Hasan, F.; Shah, A. A.; Hameed, A. - Industrial applications of microbial lipases. Enzyme and Microbial Technology, v. 39, p. 235- 251, 2006. Ho, T.J.M., Barbosa, C.C., Marra Júnior, W.D, Oliveira, C.F.A Kamimura, E.S. - Production of lipase Candida rugosa for application in anaerobic treatment of dairy watewater. Proceedings of 12° Simpósio Internacional de Iniciação Científica da USP (SIICUSP), 12 dez São Paulo/Brazil. (CDROM). (in portuguese) Kontkanen, H.; Tenkanen, M.; Fagerstrom, R.; Reinikainen, T. - Characterisation of steryl esterase activities in commercial lipase preparations. J. Biotechnol., v. 108, p.51- 59, 2004. Kumar, S.; Kikon, K.; Upadhyay, A.; Kanwar, S. S.; Gupta, R. Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expression and Purification, v. 41, p. 38-44, 2005. Li, C.; Cheng, C.; Cheng, T. Fed-batch production of lipase by Acinetobacter radioresistens using Tween80 as the carbon source. Biochemical Engineering Journal, v. 19, p. 25-31, 2004. Liu, C.; Chen, C.; Wang, Y.; Chang, J. Fermentation strategies for the production of lipase by an indigenous isolate Burkholderia sp. C20. Biochemical Engineering Journal, v. 58-59, p. 96-102, 2011. Liu, Z.; Chi, Z.; Wang, L.; Li, J. Production, purification and characterization of an extracellular lipase from Aureobasidium pulluans HN2-3 with potential application for the hydrolysis of edible oils. Biochemical Engineering Journal, v. 40, p. 445-451, 2008. Mladenoska, I.; Dimitrovski, A. – Lipase production by Geotrichum candidum – M2. Bulletin of the Chemists and Technologists of Macedonia, v. 20, p. 39- 43, 2001. Paques, F. W.; Macedo, G. A. Plant lipases from latex: properties and industrial applications. Química Nova, v. 29, n. 1, p. 93-99, 2006. Pinheiro, T. L. F.; Lipke, N. R.; Kempka, A. P.; Menoncin, S.; Oliveira, D.; Treichel, H.; Luccio, M.; Freire, D. M. G. - Response surface method to optimize the production and characterization of lipase from Penicillium verrucosum in solid state fermentation. Bioprocess and Biosystems Engineering, v. 31, n. 2, p. 119-125, 2008. Rajendran, A., Palanisamy, A., Andamp; Thangavelu, V. - Evaluation of medium components by Plackett-Burman statistical design for lipase production by Candida rugosa and kinetic modeling. Chinese Journal of Biotechnology, v. 34, p. 436–444, 2008. Rigo, E.; Ninow, J. L.; Luccio, M. D.; Oliveira, J. V.; Polloni, A. E.; Remonatto, D.; Arbter, F.; Vardanega, R.; Oliveira, D.; Treichel, H. Lipase production by solid fermentation of soybean meal with different supplements. LWT – Food Science and Technology, v. 43, p. 1132-1137, 2010. Romão, B. B. Ethanol production from acidic hydrolysed of soybean molasses. Master dissertation, Federal University of Uberlândia, 2011. Salihu, A.; Alam, M. Z.; AbdulKarim, M. I.; Salleh, H. M. Lipase production: An insight in the utilization of renewable agricultural risidues. Resources, Conservation and Recycling, v. 58, p. 36-44, 2012. Salihu, A.; Alam, M. Z.; AbdulKarim, M. I.; Salleh, H. M. Optimization of lipase production by Candida cylindracea in palm oil Mill effluent based médium using statistical experimental design. Journal of Molecular Catalysis B: Enzymatic, v. 69,p. 66-73, 2011. Silva, F. B. Ethanol production from enzymatically hydrolysed of soybean molasses. Master dissertation, Federal University of Uberlândia, 2011. Siqueira, P. F. Production of bio-ethanol from soybean molasses by Saccharomyces cerevisiae. Master Dissertation, Federal University of Paraná/ Universities of Provence and of the Mediterranean, 2007. Tan, T.; Zhang, M.; Wang, B.; Ying, C.; Deng, L. Screening of high lipase producing Candida sp. and production of lipase by fermentation. Process Biochemistry, v. 39, p. 459-465, 2003. Tommaso, G.; Moraes, B. S.; Macedo, G. C.; Silva, G. S.; Kamimura, E. S. Production of lipase from Candida rugosa using cheese whey through experimental design and surface response methodology. Food Bioprocess Technol, v. 4, n. 8, p. 1473- 1481, 2010. Treichel, H., de Oliveira, D., Mazutti, M. A., Di Luccio, M., Andamp; Oliveira, J. V. - A review on microbial lipases production. Food and Bioprocess Technology, v. 3, p. 182–196, 2010. Vargas, G. D. L. P.; Treichel, H.; Oliveira, D.; Beneti, S. C.; Freire, D. M. G.; Luccio, M. - Optimization of lipase production by Penicillium simplicissimum in soybean meal. Journal of Chemical Technology and Biotechnology, v. 83, n. 1, p. 47-54, 2007. Yang, X.; Wang, B.; Cui, F.; Tan, T. Production of lipase by repeated batch fermentation with immobilized Rhizopus arrhizus. Process Biochemistry, v. 40, n. 6, p. 2095–2103, 2005.
T1 - INFLUÊNCIA DO pH NA PRODUÇÃO DE LIPASE EM MELAÇO DE SOJA POR Geotrichum candidum.

References: v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 V. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 V. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 V. 
 v. 
 v. 
 v. 
 v. 
 v. 
 v. 
 V. 
 v. 
 v. 
 v.