Source: http://paris-neuroscience.fr/en/equipe/wavefront-engineering-microscopy
Timestamp: 2019-04-22 12:10:27+00:00

Document:
E. Ronzitti, M. Guillon, V. de Sars, and V. Emiliani.
﻿Scanless two-photon excitation of channelrhodopsin-2,﻿ E. Papagiakoumou, F. Anselmi, A. Begue, V. de Sars, J. Gluckstad, E. Y. Isacoff, and V. Emiliani Nature Methods 7, 848-854 (2010).
Holographic photolysis for multiple cell stimulation in mouse hippocampal slices, M. Zahid, M. Velez-Fort, E. Papagiakoumou, C. Ventalon, M. C. Angulo, and V. Emiliani PLoS One 5, e9431 (2010).
Temporal focusing with spatially modulated excitation.E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron Optics Express 17, 5391-5401 (2009).
Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses, E. Papagiakoumou, C. Lutz, V. De Sars, D. Oron, and V. Emiliani, Optics Express 16, 22039-22047 (2008).
Holographic photolysis of caged neurotransmittersC. Lutz, T. Otis, V. DeSars, S. Charpak, D. Digregorio, and V. Emiliani, Nature Methods 5, 821-827 (2008).
This research team is dedicated to the development of optical techniques based on wave front engineering microscopy.Traditional optical systems modify excitation light patterns through the use of lenses, diaphragms, curved mirrors, gratings, or optical fibers. They are typically limited to the generation of simple patterns (generally of circular symmetry) which are relatively difficult to change during the course of an experiment. In contrast the use of active optical elements such as micro-mirror and optical membrane devices, acousto-optical or nematic liquid crystals displays permits laser light to be dynamically redistributed by modulation of its phase or amplitude.
The active nature of these devices allows illumination patterns to be rapidly and conveniently adapted to experimental needs and greatly widens the types of experiments and studies that can be performed on biological systems.The interest of this team is to develop advanced methods for wave front engineering and to exploit the potentiality of wave front engineering microscopy in the field of neuroscience.

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