Source: http://cuir.car.chula.ac.th/dspace/handle/123456789/43692
Timestamp: 2019-04-25 08:06:28+00:00

Document:
Other Abstract: Antimicrobial peptides (AMPs) are important components of the innate immune system and function as a first line of defense against invading microorganisms. AMPs are widely distributed in all living kingdom. This research aimed to investigate the biological activity of 4 antimicrobial peptides (Antilipopolysaccharide factor (ALFSp1) and arasin-likeSp from mud crab, nisin from Lactococcus lactis and lysozyme from chicken egg white) against foodborne pathogens and crustacean pathogens. Synthetic ALFSp1 revealed the antimicrobial activity against A. viridans, M. luteus, B. subtilis, V. harveyi, and V. parahaemolyticus with Minimal Bactericidal Concentration (MBC) values of 0.39-50 &micro;M. Synthetic arasin-likeSp exhibited antimicrobial activity against A. viridans, M. luteus, B. subtilis, V. harveyi, V. parahaemolyticus, and V. anguillarum with MBC values of 3.12-50 &micro;M. The result showed that ALFSp1, arasin-likeSp exhibited strong antimicrobial against V. parahaemolyticus. Therefore, V. parahaemolyticus was chosen as foodborne pathogen for further study. Antimicrobial assay of nisin and lysozyme revealed that nisin and lysozyme did not inhibit the growth of V. parahaemolyticus. The study of synergistic bacteriostatic effect found that the ALFSp1 and arasin-likeSp had synergistic effect on inhibition of V. parahaemolyticus. The incorporation of ALFSp1 and arasin-likeSp into chitosan film was investigated. The result showed that the chitosan film containing ALFSp1 and arasin-likeSp did not produce detectable inhibition zone against V. parahaemolyticus. The addition of 10 MBC of ALFSp1 and arasin-likeSp solution to the fresh-cut shrimp during refrigerated storage (4 OC) for 24 hours was studied. The result revealed that the total bacterial count was decelerated compare with control sample.

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