Source: http://iekvm.kharkov.ua/personal/Limanskaya/Limanskaya_CV.html
Timestamp: 2019-04-21 20:44:37+00:00

Document:
B.S.: 1977-1981, Radiophysics and Biophysics, Kharkov State University, Kharkov (Ukraine).
M.S.: 1981-1982, Molecular Biophysics, Kharkov State University, Kharkov (Ukraine).
Diploma title: Study of DNA-thiotepha interaction by temperature denaturation.
Ph.D.: 2001, Institute of Molecular Biology and Genetics, National Academy of Sciences, Kiev, Ukraine.
Thesis title: Design of molecular genetic test-systems for early detection of bovine leukemia virus and preimplantation embryo sexing.
Dr.Sci.: 2011, Palladin Institute of Biochemistry, National Academy of Sciences, Kiev, Ukraine.
Thesis title: Molecular technologies for typing and detection of bacterial and viral pathogens.
2002 - present - Chief Scientist, Laboratory of Molecular Diagnostics, National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, National Academy of Agrarian Sciences of Ukraine, Kharkov, Ukraine.
2001 - present - Chief Scientist, Mechnikov Institute of Microbiology and Immunology, National Academy of Medical Sciences of Ukraine, Kharkov, Ukraine.
2002 - 2003 - Senior Scientist, Department of Microbiology, Kharkov Medical University, Kharkov, Ukraine.
1996 - 2002 - Scientist, Laboratory of Slow Infectious Diseases, Institute of Experimental and Clini- cal Veterinary Medicine, Ukrainian Academy of Agrarian Sciences, Kharkov, Ukraine.
1988 - 1997 - Researcher, Department of Biophysics, B. Verkin Institute for Low Temperatures Phy- sics & Engineering, Ukrainian Academy of Sciences, Kharkov, Ukraine.
1986 -1988 – Junior researcher, Department of Biophysics, B. Verkin Institute for Low Temperatures Physics & Engineering, Ukrainian Academy of Sciences, Kharkov, Ukraine.
1982 - 1986 - Engineer, Department of Biophysics, B. Verkin Institute for Low Temperatures Physics & Engineering, Ukrainian Academy of Sciences, Kharkov, Ukraine.
2002 - 2003 - Senior lecturer, Department of Biotechnology and Organic Chemistry, National Technical University “Kharkov Polytechnical Institute”, Kharkov, Ukraine.
1999 - Scientist, Institute of Cattle Breeding, Kharkov, Ukraine.
1995 - 1996 - Scientist, Regional Medical Genetic Center, Kharkov, Ukraine.
1993 - 1994 - Researcher, Regional Epidemiological Station, Kharkov, Ukraine.
1989 – A. Zubarev Prize of Kharkov Regional Young Scientists League.
1990 - 1992 - Research Grant, Ukrainian National Programme “Anti- HIV”: Investigation of HIV LTR by scanning tunneling microscopy.
1990 - 1991 - Research Grant, Ukrainian National Programme “Chernobyl”: PCR –detection of viral diseases.
1993 – Individual J. Soros Grant.
1996 - 2003 - Research Grants of Ukrainian Ministry of Health and Ukrainian Academy of Medical Sciences at study of genome organization of viral and bacterial infectious agents.
Bioinformatics – search of molecular markers for structural differentiation of viruses and bacteria genomes, molecular evolution and phylogenetic analysis.
Molecular retrovirology, molecular biology and molecular medicine – development of molecular technologies for detection and typing human and animal pathogens, gene regulation, sexing of animal embryos, preimplantation molecular diagnostics, protein - DNA interaction, mechanisms of DNA – drug specific interaction, thermodynamics of DNA and DNA – drug interaction.
Nanobiology – nanobiotechnological approaches for visualization and manipulation by single biomolecules and cells, atomic force microscopy of cells, biopolymers, nucleic acids, protein-nucleic acids complexes.
Professional experience Purification of nucleic acids; equilibrium centrifugation; HPLC and conventional liquid chromatogra-phy; optical spectroscopy; thermodynamics of DNA melting; conventional gel electrophoresis; polymerase chain reaction; design of molecular genetic test-systems for detection and differentiation of microorganisms; detection of human and animal viruses and bacteria.
1.Pyatigorskaya T., Zhilkova O., Arkhangelova N., Shelkovsky V., Sukhodub L. Study of thiotepha stability in aqueous and aqueous -salt solutions. - Chemical Pharmaceutical Journal. – 1984, N 3:343-349.
2. Pyatigorskaya T., Zhilkova O., Shelkovsky V., Kosevich M., Sukhodub L. Study of thiotepha trans-formation products in aqueous solutions by thin layer chromatography and mass spectrometry. – Chemical Pharmaceutical Journal. – 1985, N 10: 1235-1241.
3. Sukhodub L., Shelkovsky V., Kosevich M., Pyatigorskaya T., Zhilkova O. Mass spectroscopic stu-dy of interaction of thiotepha with nucleic acids. – Proceedings of Academy of Sciences of USSR. – 1985. – V. 283, N 3: 714-716.
4. Pyatigorskaya T., Zhilkova O., Muravjeva L., Sukhodub L. DNA interaction with anticancer drug thiotepha. – Molecular Biology. – 1986. – V. 20, N 2: 423-429.
5. Sukhodub L., Shelkovsky V., Kosevich M., Pyatigorskaya T., Zhilkova O. Nucleic acid base comp-lexes with thiotepa as revealed by field ionization mass spectrometry. – Biomedical and Environmen- tal Mass Spectrometry. – 1986. – V.13, N 4: 167-170.
6. Pyatigorskaya T., Zhilkova O., Shelkovsky V., Arkhangelova N., Grizodub A., Sukhodub L. Hydro-lysis of 1,1’,1”-phosphinothioylidinetriaziridine (Thiotepha) in aqueous solution. – Biomedical and Environmental Mass Spectrometry. – 1987. – V. 14, N 4: 143-148.
7. Sukhodub L., Andrievsky G., Pyatigorskaya T., Kosevich M., Zhilkova O. Application of mass spectrometry with atom ionization for product identification of DNA-thiotepha interaction. – Bioorganic Chemistry. – 1988. – V. 14, N 12:1698-1699.
8. Sukhodub L., Kosevich M., Shelkovsky V., Pyatigorskaya T., Zhilkova O. Direct observation of nucleic acid bases adducts with thiotepha by ionization mass spectrometry. – Biophysics. – 1990. – V. 35, N 4: 549-552.
9. Limanskaya O., Limanskii A. Universal test-system for PCR sexing of cattle, sheep, goat and pig embryos. – Journal Biology of Reproduction. – 1999. – V. 60, Suppl. 1. – P. 195.
10. Limanskaya O., Limanskii A., Bezugly N. Preimplantation embryo sexing of farm animals by po-lymerase chain reaction. – Russian J. of Biotechnology. – 2000, N 2: 66-72.
11. Limanskii A., Limanskaya O. The methods of molecular genetics as the basis for the programs of bovine leukosis eradication. – Russian J. of Biotechnology. – 2001, N 3: 187-193.
12. Limanskaya O., Limanskii A. Analysis of primer sets for PCR-detection of provirus DNA of bo- vine leukemia virus and human immunodeficiency virus. – Biopolymers and Cell. – 2002. - V. 18, N 2: 124-130.
13. Limanskii A., Limanskaya O. Comparison of primer sets for detection of bovine leukemia virus by polymerase chain reaction. – Bulletin of the Veterinary Institute in Pulawy (Poland). – 2002. – V. 46, N 1: 27-36.
14. Lymans’kiy A.P., Lymans'ka O. Study of microorganisms genome DNA by atomic force micro-scopy. – Cytology and Genetics. – 2002. – V. 36, N 4: 30-36.
15. Lymans'kyy A., Limans'ka O. Visualization of cyanobacteria in aqueous solution by atomic force microscopy. – Cytology and Genetics. – 2003. – V. 37, N 1: 68-71.
16. Limanskii A., Geue L., Limanskaya O., Beier D. Typing of bovine leukemia virus circulated in Ukraine. – Problems of Virology. – 2004, N 1: 39-44.
17.Limanskii A., Minukhin V., Limanskaya O., Pavlenko N., Mishina M., Tsyganenko A. Species-specific detection of Proteus vulgaris and Proteus mirabilis by polymerase chain reaction. – J. of Mic- robiology, Epidemiology and Immunobiology. – 2005, N 3: 33-39.
18. Limanskaya O., Rola M., Bicka L., Kuzmak J., Limanskii A. Detection of bovine immunodeficiency virus proviral DNA by polymerase chain reaction. – Problems of Virology. – 2005. – V. 50, N 2: 38-43.
19. Limanskaya O.Y., Limanskaya L.A., Limanskii A.P. Compaction of supercoiled DNA on modified aminomica. – Biopolymers and Cell. – 2006. – V. 22, N 1: 18-28.
20. Limanskaya O., Limanskii A. Imaging of T7 RNA polymerase elongation complexes by atomic force microscopy. – Molecular Biology. – 2008 – V. 42, N 3: 469-477.
21. Limanskaya O., Limanskii A. Imaging compaction of single supercoiled DNA molecules by atomic force microscopy. – General Physiology and Biophysics. – 2008. – V. 27, N 4: 322-337.
22. Limanskaya O.Yu., Limanskii A.P. Distribution of potentially hairpin-loop structures in the genome of bovine retroviruses. – Problems of Virology. – 2009. – V. 54, N 4: 27-32.
23. Limanskaya O.Yu. Species-specific detection of Mycobacterium tuberculosis complex. – Tuberculosis and Lung Diseases. – 2009, N 10: 49-55.
24. Limanskaya O.Yu. Bioinformatic analysis of inverted repeats of coronaviruses genome. – Biopolymers and Cell. – 2009. – V. 25, N 4: 307-314.
25. Limanskaya O.Yu. Polypurine/polypyrimidine sequences with the potential of forming triplexes in the proviral DNA of bovine retroviruses. – Cytology and Genetics. – 2010. – V. 44, N 1: 10-18.
26. O.Yu. Limanskaya, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, X. Wu, J. Zhang, T.V. Fesenko, V.A. Pokrovskiy, A.P. Limanskii. Identification of wild-type Mycobacterium tuberculosis isolates and point mutations associated with isoniazid resistance. – Molecular Biology. – 2010. – Vol. 44, N 4: 559–567.
27. O.Yu. Limanskaya, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, A.P. Limanskii Detection of wild type isolates and isoniazid-resistant mycobacteria tuberculosis. – Tuberculosis and Lung Diseases. – 2010, N 9: 45-51.
28. O.Yu. Limanskaya, T.N. Fesenko, V.A. Pokrovskiy, T.N. Mukhina, V.N. Stepanshina, I.G. Shemyakin, A.P. Limanskii. Characterization of oligonucleotides with LNA monomers for PCR detection of point mutations in Mycobacterium tuberculosis genome. – Biochemistry (Moscow). Supplement Series B: Biomedical Chemistry. – 2011. – V. 5, N 2: 144–151.
29.Limanskaya O., Murtazaeva L., Limanskii A. Species-specific detection of causative agent of anthrax // Biotechnology. – 2012. – V. 5, N 1. – P. 92-99.
30.O. Limanskaya, A. Limanskii. Study of elongation complexes for T7 RNA polymerase // Biophysics. – 2012. – V. 57, N 4. – P. 428-441.
31.Limanskaya O., Murtazaeva L., Klee S., Limanskii A. Detection of anthrax causative agent by real-time polymerase chain reaction // Biotechnology. – 2012. – V. 5, N 5. – P. 65-71.
32.O. Limanskaya, L. Murtazaeva, A. Limanskii. Search for the new potential RNA thermometers in the genome of Salmonella enterica // Microbiology. – 2013. – V. 82, N 1. – P. 69-78.
33.O.Yu. Limanskaya, L.A. Murtazaeva, and A.P. Limanskii. Potential thermosensitive riboswitches in the genome of Salmonella. - Cytology and Genetics. – 2013. – V. 47, N 5. – P. 268–275.
1. Ukraine Patent 36260: A.P. Limanskii, O.Yu. Limanskaya. Detection method of bovine leukemia virus 25.11.1999; Publ.16.04.2001. Bul. N 3.
2. Ukraine Patent 33324: A.P. Limanskii, O.Yu. Limanskaya, V.F. Babkin, N.D. Bezugly, Yu.L. Vo-lyansky, I.Yu. Kuchma, A.I. Dobroskok, M.V. Babkin. Method of animal embryos sexing 10.02.1999. Publ.15.02.2001. Bul. N 1.
3. Ukraine Patent 40310: A.P. Limanskii, A.Yu. Volyansky, O.Yu. Limanskaya. Detection method of Listeria monocytogenes, L. Ivanovii, L.seeligeri, L.welshimeri 01.12.2000; Publ.16.07.2001.Bul. N 6.
4. Ukraine Patent 40371: A.Yu. Volyansky, A.P. Limanskii, O.Yu. Limanskaya. Detection method of pathogenic Listeria sp. 22.12.2000; Publ.16.07.2001. Bul. N 6.
5. Ukraine Patent 39715: O.Yu. Limanskaya, A.Yu. Volyansky, A.P. Limanskii. Typing method of pathogenic and nonpathogenic Listeria sp. 03.01.2001; Publ.15.06.2001. Bul. N 5.
6. Ukraine Patent 66255: Tsyganenko A., Limanskaya O., Limanskii A., Mishina M., Minukhin V. Detection method of Proteus vulgaris 09.09.2003; Publ.15.04. 2004. Bul. N 4.
7. Ukraine Patent 66291: Limanskii A., Limanskaya O., Volyanskii A., Dobroskok A., Novikov S., Manolov V., Volyanskaya N. Detection method of bovine immunodeficiency virus 13.10.2003; Publ.15.04. 2004. Bul. N 4.
8. Ukraine Patent 13571: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Dratch M., Li-manskaya L., Klimov O. Method of mica (as substrate for atomic force microscopy) modification 22.08.2005; Publ.17.04.2006. Bul. N 4.
9. Ukraine Patent 17942: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Dratch M., Limanskaya L., Klimov O. Method of stretching linear and supercoiled DNA molecules 25.04.2006; Publ. 16.10.2006. Bul. N 10.
10. Ukraine Patent 17981: Limanskii A., Limanskaya O., Volyanskii A., Rudenko S., Kuchma I., Limanskaya L., Pugach N. Method for compression of linear and supercoiled DNA molecules 03.05. 2006; Publ.16.10.2006. Bul. N 10.
11. Ukraine Patent 80264: Tsyganenko A., Limanskaya O., Limanskii A., Minukhin V. Detection method of Proteus vulgaris and Proteus mirabilis 10.06. 2004; Publ.10.09.2007. Bul. N 14.
12. Ukraine Patent 34435: Limanskii A., Limanskaya O., Limanskaya L. Differentiation method of mycobacteria tuberculosis complex 17.03. 2008; Publ.11.08.2008. Bul. N 15.
13. Ukraine Patent 70049: Limanskaya O., Murtazaeva L., Limanskii A. Detection method of anthrax causative agent 14.11.2011; Publ. 25.05.2012. Bul. N 10.

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