Source: http://www.asmscience.org/content/book/10.1128/9781555816728.chap26
Timestamp: 2019-04-24 12:00:49+00:00

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This chapter deals with aerobically growing, asporogenous, irregularly shaped, non-partially acid-fast, gram-positive rods generally called "coryneforms." The bacteria discussed in the chapter all belong to the class Actinobacteria, the genera of which are characterized by specific 16S rRNA gene signature nucleotides. The 16S rRNA gene sequencing data demonstrate that the genera Corynebacterium and Turicella are more closely related to the partially acid-fast bacteria and to the genus Mycobacterium than to the other coryneform organisms covered in the chapter. The chapter discusses descriptions of genera and species. Detection of antibodies directed against diphtheria toxin is the only established serologic test for coryneform bacteria. Toxin neutralization assays using a Vero cell culture system have been replaced mainly by enzyme immunoassays. The susceptibility patterns for each taxon were given with the descriptions of each taxon. Since the antimicrobial susceptibility of coryneform bacteria is not predictable in every case, susceptibility testing should always be performed with clinically significant isolates.
Gram stain morphologies of Corynebacterium diphtheriae ATCC 14779 after 48 h of incubation (a), Turicella otitidis DSM 8821 (48 h) (b), Dermabacter hominis ATCC 51325 (48 h) (c), Gardnerella vaginalis ATCC 14018 (48 h) (d), Corynebacterium durum DMMZ 2544 (72 h) (e), and Corynebacterium matruchotii ATCC 14266 (24 h) (f), Corynebacterium aurimucosum HC-NML 91-0032 (24 h) (g), and black-pigmented Rothia dentocariosa HC-NML 77-0298 (24 h) (h).
CAMP reactions of different coryneform bacteria after 24 h. (Top) C. glucuronolyticum DMMZ 891 (positive reaction); (middle) C. diphtheriae ATCC 14779 (negative reaction); (bottom) A. haemolyticum ATCC 9345 (CAMP inhibition reaction). The vertical streak is S. aureus ATCC 25923.
Colony morphologies of different coryneform bacteria after 48 h of incubation on SBA. (a) C. amycolatum LCDC 91-0077; (b) C. diphtheriae ATCC 14779; (c) C. mucifaciens LCDC 97-0202; (d) C. striatum ATCC 6940; (e) D. hominis ATCC 51325; (f ) R. dentocariosa LCDC 95-0154; (g) C. aurimucosum HC-NML 91-0032 (after 96 h); (h) black-pigmented Rothia dentocariosa HC-NML 77- 0298 (after 96 h); (i) C. diphtheriae biotype gravis colonies on a Tinsdale agar plate. The last photo was kindly provided by C. Hinnebusch and M. Cohen, UCLA School of Medicine, Los Angeles, CA.
1. Achermann, Y.,, A. Trampuz,, F. Moro,, J. Wüst,, and M. Vogt. 2009. Corynebacterium bovis shoulder prosthetic joint infection: the first reported case. Diagn. Microbiol. Infect. Dis. 64: 213– 215.
2. Adekambi, T.,, T. M. Shinnick,, D. Raoult,, and M. Drancourt. 2008. Complete rpoB gene sequencing as a suitable supplement to DNA-DNA hybridization for bacterial species and genus delineation. Int. J. Syst. Evol. Microbiol. 58: 1807– 1814.
3. Aravena-Roman, M.,, C. Spröer,, B. Sträubler,, T. Inglis,, and A. F. Yassin. 2010. Corynebacterium pilbarense sp. nov., a non-lipophilic corynebacterium isolated from a human ankle aspirate. Int. J. Syst. Evol. Microbiol. 60: 1484– 1487.
4. Aubel, D.,, F. N. R. Renaud,, and J. Freney. 1997. Genomic diversity of several Corynebacterium species identified by amplification of the 16S-23S rRNA gene spacer regions. Int. J. Syst. Bacteriol. 47: 767– 772.
5. Auzias, A.,, C. Bollet,, R. Ayari,, M. Drancourt,, and D. Raoult. 2003. Corynebacterium freneyi bacteremia. J. Clin. Microbiol. 41: 2777– 2778.
6. Bernard, K. A. 2005. Corynebacterium species and coryneforms: an update on taxonomy and diseases attributed to these taxa. Clin. Microbiol. Newsl. 27: 9– 18.
7. Bernard, K. A.,, M. Bellefeuille,, and E. P. Ewan. 1991. Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods. J. Clin. Microbiol. 29: 83– 89.
8. Bernard, K. A.,, C. Munro,, D. Wiebe,, and E. Ongsansoy. 2002. Characteristics of rare or recently described Corynebacterium species recovered from human clinical material in Canada. J. Clin. Microbiol. 40: 4375– 4381.
9. Bernard, K. A.,, D. Wiebe,, T. Burdz,, A. Reimer,, B. Ng,, C. Singh,, S. Schindle,, and A. L. Pacheco. 2009. Assignment of Brevibacterium stationis (ZoBell and Upham 1994) Breed 1953 to the genus Corynebacterium as Corynebacterium stationis comb. nov. and emended description of the genus Corynebacterium to include isolates which can alkalinize citrate. Int. J. Syst. Evol. Microbiol. 60: 874–879..
10. Boltin, D.,, M. Katzir,, V. Bugoslavsky,, I. Yalashvili,, T. Brosh-Nissimov,, M. Fried,, and O. Elkayam. 2009. Corynebacterium striatum—a classic pathogen eluding diagnosis. Eur. J. Intern. Med. 20: e49– e52.
11. Bonmarin, I.,, N. Guiso,, A. Le Flèche-Matéos,, O. Patey,, A. D. Patrick,, and D. Levy-Bruhl. 2009. Diphtheria: a zoonotic disease in France? Vaccine 27: 4196– 4200.
12. Boumghar-Bourtchai, L.,, H. Chardon,, B. Malbruny,, S. Mezghani,, R. Leclercq,, and A. Dhalluin. 2009. Resistance to macrolides by ribosomal mutation in clinical isolates of Turicella otitidis. Int. J. Antimicrob. Agents 34: 274– 277.
13. Brown, J. M.,, R. P. Frazier,, R. E. Morey,, A. G. Steigerwalt,, G. J. Pellegrini,, M. I. Daneshvar,, D. G. Hollis,, and M. M. McNeil. 2005. Phenotypic and genetic characterization of clinical isolates of CDC coryneform group A-3: proposal of a new species of Cellulomonas, Cellulomonas denverensis sp. nov. J. Clin. Microbiol. 43: 1732– 1737.
14. Brown, J. M.,, A. G. Steigerwalt,, R. E. Morey,, M. I. Daneshvar,, L. J. Romero,, and M. M. McNeil. 2006. Characterization of clinical isolates previously identified as Oerskovia turbata: proposal of Cellulosimicrobium funkei sp. nov. and emended description of the genus Cellulosimicrobium. Int. J. Syst. Evol. Microbiol. 56: 801– 804.
15. Cassiday, P. K.,, L. C. Pawloski,, T. Tiwari,, G. N. Sanden,, and P. P. Wilkins. 2008. Analysis of toxigenic Corynebacterium ulcerans strains revealing potential for false-negative real-time PCR results. J. Clin. Microbiol. 46: 331– 333.
16. Catlin, B. W. 1992. Gardnerella vaginalis: characteristics, clinical considerations, and controversies. Clin. Microbiol. Rev. 5: 213– 237.
17. Cerdeno-Tarraga, A. M.,, A. Efstratiou,, L. G. Dover,, M. T. G. Holden,, M. Pallen,, S. D. Bentley,, G. S. Besra,, C. Churcher,, K. D. James,, A. De Zoysa,, T. Chillingworth,, A. Cronin,, L. Dowd,, T. Feltwell,, N. Hamlin,, S. Holroyd,, K. Jagels,, K. Moule,, M. A. Quail,, E. Rabbinowitsch,, K. M. Rutherford,, N. R. Thomson,, L. Unwin,, S. Whitehead,, B. G. Barrell,, and J. Parkhill. 2003. The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC 13129. Nucleic Acid Res. 31: 6516– 6523.
18. Clinical and LaboratoryStandards Institute. 2010. Methods for antimicrobial dilutionand disk susceptibility testing of infrequently isolated or fastidious bacteria. Document M45-A2. Clinical and Laboratory Standards Institute, Wayne, PA.
19. Collins, M. D. 1982. Reclassification of Bacterionema matruchotii (Mendel) in the genus Corynebacterium, as Corynebacterium matruchotii comb. nov. Zentralbl. Bakteriol. Mikrobiol. Hyg. Abt. 1 Orig. C 3: 364– 367.
20. Collins, M. D.,, K. A. Bernard,, R. A. Hutson,, B. Sjöden,, A. Nyberg,, and E. Falsen. 1999. Corynebacterium sundsvallense sp. nov., from human clinical specimens. Int. J. Syst. Bacteriol. 49: 361– 366.
21. Collins, M. D.,, R. A. Burton,, and D. Jones. 1988. Corynebacterium amycolatum sp. nov., a new mycolic acid-less Corynebacterium species from human skin. FEMS Microbiol. Lett. 49: 349– 352.
22. Collins, M.D.,, and C. S. Cummins,. 1986. Genus Corynebacterium, p. 1266– 1276. In P. H. A. Sneath,, N. S. Mair,, M. E. Sharpe,, and J. G. Holt (ed.), Bergey’s Manual of Systematic Bacteriology, vol. 2. The Williams & Wilkins Co., Baltimore, MD.
23. Collins, M. D.,, E. Falsen,, E. Akervall,, B. Sjöden,, and A. Alvarez. 1998. Corynebacterium kroppenstedtii sp. nov., a novel corynebacterium that does not contain mycolic acids. Int. J. Syst. Bacteriol. 48: 1449– 1454.
24. Collins, M. D.,, R. A. Hutson,, V. Baverud,, and E. Falsen. 2000. Characterization of a Rothia-like organism from a mouse: description of Rothia nasimurium sp. nov. and reclassification of Stomatococcus mucilaginosus as Rothia mucilaginosa comb. nov. Int. J. Syst. Evol. Microbiol. 50: 1247– 1251.
25. Dallman, T.,, S. Neal,, J. Green,, and A. Efstratiou. 2008. Development of an online database for diphtheria molecular epidemiology under the remit of the DIPNET project. Euro Surveill. 13: 18865.
26. Daneshvar, M. I.,, D. G. Hollis,, R. S. Weyant,, J. G. Jordan,, J. P. MacGregor,, R. E. Morey,, A. M. Whitney,, D. J. Brenner,, A. G. Steigerwalt,, L. O. Helsel,, P. M. Raney,, J. B. Patel,, P. N. Levett,, and J. M. Brown. 2004. Identification of some charcoal-black-pigmented CDC fermentative coryneform group 4 isolates as Rothia dentocariosa and some as Corynebacterium aurimucosum: proposal of Rothia dentocariosa emend. Georg and Brown 1967, Corynebacterium aurimucosum emend. Yassin et al. 2002, and Corynebacterium nigricans Shukla et al. 2003 pro synon. Corynebacterium aurimucosum. J. Clin. Microbiol. 42: 4189– 4198.
27. De Briel, D.,, F. Couderc,, P. Riegel,, F. Jehl,, and R. Minck. 1992. High-performance liquid chromatography of corynomycolic acids as a tool in identification of Corynebacterium species and related organisms. J. Clin. Microbiol. 30: 1407– 1417.
28. Devriese, L. A.,, P. Riegel,, J. Hommez,, M. Vaneechoutte,, T. de Baere,, and F. Haesebrouck. 2000. Identification of Corynebacterium glucuronolyticum strains from the urogenital tract of humans and pigs. J. Clin. Microbiol. 38: 4657– 4659.
29. De Zoysa, A.,, A. Efstratiou,, and P. M. Hawkey. 2005. Molecular characterization of diphtheria toxin repressor (dtxR) genes present in nontoxigenic Corynebacterium diphtheriae strains isolated in the United Kingdom. J. Clin. Microbiol. 43: 223– 228.
30. De Zoysa, A.,, P. Hawkey,, A. Charlett,, and A. Efstratiou. 2008. Comparison of four molecular typing methods for characterization of Corynebacterium diphtheriae: transcontinental spread of C. diphtheriae based on BstEII rRNA gene profiles. J. Clin. Microbiol. 46: 3626– 3635.
31. De Zoysa, A.,, P. M. Hawkey,, K. Engler,, R. George,, G. Mann,, W. Reilly,, D. Taylor,, and A. Efstratiou. 2005. Characterization of toxigenic Corynebacterium ulcerans strains isolated from humans and domestic cats in the United Kingdom. J. Clin. Microbiol. 43: 4377– 4381.
32. Efstratiou, A.,, K. H. Engler,, C. S. Dawes,, and D. Sesardic. 1998. Comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic corynebacteria. J. Clin. Microbiol. 36: 3173– 3177.
33. Efstratiou, A.,, K. H. Engler,, I. K. Mazurova,, T. Glushkevich,, J. Vuopio-Varkila,, and T. Popovic. 2000. Current approaches to the laboratory diagnosis of diphtheria. J. Infect. Dis. 181( Suppl. 1): S138– S145.
34. Efstratiou, A.,, and R. C. George. 1999. Laboratory guidelines for the diagnosis of infections caused by Corynebacterium diphtheriae and C. ulcerans. WHO Commun. Dis. Public Health 2: 250– 257.
35. Eguchi, H.,, T. Kuwahara,, T. Miyamoto,, H. Nakayama- Imaohji,, M. Ichimura,, T. Hayashi,, and H. Shiota. 2008. High-level fluoroquinolone resistance in ophthalmic clinical isolates belonging to the species Corynebacterium macginleyi. J. Clin. Microbiol. 46: 527– 532.
36. Elsayed, S.,, and K. Zhang. 2005. Bacteremia caused by Janibacter melonis. J. Clin. Microbiol. 43: 3537– 3539.
37. Engler, K. H.,, T. Glushkevich,, I. K. Mazarova,, R. C. George,, and A. Efstratiou. 1997. A modified Elek test for detection of toxigenic corynebacteria in the diagnostic laboratory. J. Clin. Microbiol. 35: 495– 498.
38. Evtushenko, L. I.,, L. V. Dorofeeva,, S. A. Subbotin,, J. R. Cole,, and J. M. Tiedje. 2000. Leifsonia poae gen. nov., sp. nov., isolated from nematode gall on Poa annua, and reclassification of ‘ Corynebacterium aquaticum’ Leifson 1962 as Leifsonia aquatica (ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli (Davis et al. 1984) gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 50: 371– 380.
39. Farrow, J. A. E.,, S. Wallbanks,, and M. D. Collins. 1994. Phylogenetic interrelationships of round-spore-forming bacilli containing cell walls based on lysine and the non-sporeforming genera Caryophanon, Exiguobacterium, Kurthia, and Planococcus. Int. J. Syst. Bacteriol. 44: 74– 82.
40. Fernández-Natal, M. I.,, J. A. Sáez-Nieto,, R. Fernández- Roblas,, M. Asencio,, S. Valdezate,, S. Lapeña,, R. H. Rodríguez-Pollán,, J. M. Guerra,, J. Blanco,, F. Cachón,, and F. Soriano. 2008. The isolation of Corynebacterium coyleae from clinical samples: clinical and microbiological data. Eur. J. Clin. Microbiol. Infect. Dis. 27: 177– 184.
41. Fernández-Natal, M. I.,, J. A. Sáez-Nieto,, S. Valdezate,, R. H. Rodríguez-Pollán,, S. Lapeña,, F. Cachón,, and F. Soriano. 2009. Isolation of Corynebacterium ureicelerivorans from normally sterile sites in humans. Eur. J. Clin. Microbiol. Infect. Dis. 28: 677– 681.
42. Fernandez-Roblas, R.,, H. Adames,, N. Z. Martín-de-Hijas,, D. G. Almeida,, I. Gadea,, and J. Esteban. 2009. In vitro activity of tigecycline and 10 other antimicrobials against clinical isolates of the genus Corynebacterium. Int. J. Antimicrob. Agents 33: 453– 455.
43. Feurer, C.,, D. Clermont,, F. Bimet,, A. Candrea,, M. Jackson,, P. Glaser,, C. Bizet,, and C. Dauga. 2004. Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov. Int. J. Syst. Evol. Microbiol. 54: 1055– 1061.
44. Fredricks, D. N.,, T. L. Fiedler,, K. K. Thomas,, B. B. Oakley,, and J. M. Marrazzo. 2007. Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. J. Clin. Microbiol. 45: 3270– 3276.
45. Funke, G.,, M. Altwegg,, L. Frommelt,, and A. von Graevenitz. 1999. Emergence of related nontoxigenic C. diphtheriae biotype mitis strains in Western Europe. Emerg. Infect. Dis. 5: 477– 480.
46. Funke, G.,, M. Aravena-Roman,, and R. Frodl. 2005. First description of Curtobacterium spp. isolated from human clinical specimens. J. Clin. Microbiol. 43: 1032– 1036.
47. Funke, G.,, K. A. Bernard,, C. Bucher,, G. E. Pfyffer,, and M. D. Collins. 1995. Corynebacterium glucuronolyticum sp. nov. isolated from male patients with genitourinary infections. Med. Microbiol. Lett. 4: 204– 215.
48. Funke, G.,, and A. Carlotti. 1994. Differentiation of Brevibacterium spp. encountered in clinical specimens. J. Clin. Microbiol. 32: 1729– 1732.
49. Funke, G.,, A. Efstratiou,, D. Kuklinska,, R. A. Hutson,, A. De Zoysa,, K. H. Engler,, and M. D. Collins. 1997. Corynebacterium imitans sp. nov. isolated from patients with suspected diphtheria. J. Clin. Microbiol. 35: 1978– 1983.
50. Funke, G.,, R. Englert,, R. Frodl,, K. A. Bernard,, and S. Stenger. 2010. Corynebacterium canis sp. nov., isolated from a wound infection caused by a dog bite. Int. J. Syst. Evol. Microbiol. 60: 2544– 2547.
51. Funke, G.,, E. Falsen,, and C. Barreau. 1995. Primary identification of Microbacterium spp. encountered in clinical specimens as CDC coryneform group A-4 and A-5 bacteria. J. Clin. Microbiol. 33: 188– 192.
52. Funke, G.,, and R. Frodl. 2008. Comprehensive study of Corynebacterium freneyi strains and extended and emended description of Corynebacterium freneyi Renaud, Aubel, Riegel, Meugnier, and Bollet 2001. J. Clin. Microbiol. 46: 638– 643.
53. Funke, G.,, R. Frodl,, K. A. Bernard,, and R. Englert. 2009. Corynebacterium freiburgense sp. nov., isolated from a wound from a dog bite. Int. J. Syst. Evol. Microbiol. 59: 2054– 2057.
54. Funke, G.,, R. A. Hutson,, K. A. Bernard,, G. E. Pfyffer,, K. Weiss,, M. D. Collins,, and G. Wauters. 1996. Isolation of Arthrobacter spp. from clinical specimens and description of Arthrobacter cumminsii sp. nov. and Arthrobacter woluwensis sp. nov. J. Clin. Microbiol. 34: 2356– 2363.
55. Funke, G.,, R. A. Hutson,, M. Hilleringmann,, W. R. Heizmann,, and M. D. Collins. 1997. Corynebacterium lipophiloflavum sp. nov. isolated from a patient with bacterial vaginosis. FEMS Microbiol. Lett. 150: 219– 224.
56. Funke, G.,, P. A. Lawson,, K. A. Bernard,, and M. D. Collins. 1996. Most Corynebacterium xerosis strains identified in the routine clinical laboratory correspond to Corynebacterium amycolatum. J. Clin. Microbiol. 34: 1124– 1128.
57. Funke, G.,, P. A. Lawson,, and M. D. Collins. 1995. Heterogeneity within Centers for Disease Control and Prevention coryneform group ANF-1-like bacteria and description of Corynebacterium auris sp. nov. Int. J. Syst. Bacteriol. 45: 735– 739.
58. Funke, G.,, P. A. Lawson,, and M. D. Collins. 1997. Corynebacterium mucifaciens sp. nov., an unusual species from human clinical material. Int. J. Syst. Bacteriol. 47: 952– 957.
59. Funke, G.,, P. A. Lawson,, and M. D. Collins. 1998. Corynebacterium riegelii sp. nov., an unusual species isolated from female patients with urinary tract infections. J. Clin. Microbiol. 36: 624– 627.
60. Funke, G.,, P. A. Lawson,, F. S. Nolte,, N. Weiss,, and M. D. Collins. 1998. Aureobacterium resistens sp. nov. exhibiting vancomycin resistance and teicoplanin susceptibility. FEMS Microbiol. Lett. 158: 89– 93.
61. Funke, G.,, C. R. Osorio,, R. Frei,, P. Riegel,, and M. D. Collins. 1998. Corynebacterium confusum sp. nov., isolated from human clinical specimens. Int. J. Syst. Bacteriol. 48: 1291– 1296.
62. Funke, G.,, M. Pagano-Niederer,, and W. Bernauer. 1998. Corynebacterium macginleyi has to date been isolated exclusively from conjunctival swabs. J. Clin. Microbiol. 36: 3670– 3673.
63. Funke, G.,, M. Pagano-Niederer,, B. Sjöden,, and E. Falsen. 1998. Characteristics of Arthrobacter cumminsii, the most frequently encountered Arthrobacter species in human clinical specimens. J. Clin. Microbiol. 36: 1539– 1543.
64. Funke, G.,, C. Pascual Ramos,, and M. D. Collins. 1995. Identification of some clinical strains of CDC coryneform group A-3 and group A-4 bacteria as Cellulomonas species and proposal of Cellulomonas hominis sp. nov. for some group A-3 strains. J. Clin. Microbiol. 33: 2091– 2097.
65. Funke, G.,, C. Pascual Ramos,, and M. D. Collins. 1997. Corynebacterium coyleae sp. nov., isolated from human clinical specimens. Int. J. Syst. Bacteriol. 47: 92– 96.
66. Funke, G.,, C. Pascual Ramos,, J. F. Fernandez-Garayzabal,, N. Weiss,, and M. D. Collins. 1995. Description of humanderived Centers for Disease Control coryneform group 2 bacteria as Actinomyces bernardiae sp. nov. Int. J. Syst. Bacteriol. 45: 57– 60.
67. Funke, G.,, K. Peters,, and M. Aravena-Roman. 1998. Evaluation of the RapID CB Plus system for identification of coryneform bacteria and Listeria spp. J. Clin. Microbiol. 36: 2439– 2442.
68. Funke, G.,, V. Pünter,, and A. von Graevenitz. 1996. Antimicrobial susceptibility patterns of some recently established coryneform bacteria. Antimicrob. Agents Chemother. 40: 2874– 2878.
69. Funke, G.,, F. N. R. Renaud,, J. Freney,, and P. Riegel. 1997. Multicenter evaluation of the updated and extended API (RAPID) Coryne database 2.0. J. Clin. Microbiol. 35: 3122– 3126.
70. Funke, G.,, S. Stubbs,, G. E. Pfyffer,, M. Marchiani,, and M. D. Collins. 1994. Characteristics of CDC group 3 and group 5 coryneform bacteria isolated from clinical specimens and assignment to the genus Dermabacter. J. Clin. Microbiol. 32: 1223– 1228.
71. Funke, G.,, S. Stubbs,, G. E. Pfyffer,, M. Marchiani,, and M. D. Collins. 1994. Turicella otitidis gen. nov., sp. nov., a coryneform bacterium isolated from patients with otitis media. Int. J. Syst. Bacteriol. 44: 270– 273.
72. Funke, G.,, A. von Graevenitz,, J. Clarridge III,, and K. A. Bernard. 1997. Clinical microbiology of coryneform bacteria. Clin. Microbiol. Rev. 10: 125– 159.
73. Funke, G.,, A. von Graevenitz,, and N. Weiss. 1994. Primary identification of Aureobacterium spp. isolated from clinical specimens as “Corynebacterium aquaticum.” J. Clin. Microbiol. 32: 2686– 2691.
74. Gneiding, K.,, R. Frodl,, and G. Funke. 2008. Identities of Microbacterium spp. encountered in human clinical specimens. J. Clin. Microbiol. 46: 3646– 3652.
75. Gomez-Garces, J. L.,, J. I. Alos,, and J. Tamayo. 2007. In vitro activity of linezolid and 12 other antimicrobials against coryneform bacteria. Int. J. Antimicrob. Agents 29: 688– 692.
76. Grimont, P. A. D.,, F. Grimont,, A. Efstratiou,, A. De Zoysa,, I. Mazurova,, C. Ruckly,, M. Lejay-Collin,, S. Martin- Delautre,, B. Regnault, and members of the European Laboratory Working Group on Diphtheria. 2004. International nomenclature for Corynebacterium diphtheriae ribotypes. Res. Microbiol. 155: 162– 166.
77. Hall, V.,, M. D. Collins,, R. A. Hutson,, P. A. Lawson,, E. Falsen,, and B. Duerden. 2003. Corynebacterium atypicum sp. nov., from a human clinical source, does not contain corynomycolic acids. Int. J. Syst. Evol. Microbiol. 53: 1065– 1068.
78. Hauser, D.,, M. R. Popoff,, M. Kiredjian,, P. Boquet,, and F. Bimet. 1993. Polymerase chain assay for diagnosis of potentially toxigenic Corynebacterium diphtheriae strains: correlation with ADP-ribosylation activity assay. J. Clin. Microbiol. 31: 2720– 2723.
79. Hollis, D. G.,, and R. E. Weaver. 1981. Gram-positive organisms: a guide to identification. Special Bacteriology Section, Centers for Disease Control, Atlanta, GA.
80. Hommez, J.,, L. A. Devriese,, M. Vaneechoutte,, P. Riegel,, P. Butaye,, and F. Haesebrouck. 1999. Identification of nonlipophilic corynebacteria isolated from dairy cows with mastitis. J. Clin. Microbiol. 37: 954– 957.
81. Jones, D.,, and M. D. Collins. 1988. Taxonomic studies on some human cutaneous coryneform bacteria: description of Dermabacter hominis gen. nov., sp. nov. FEMS Microbiol. Lett. 51: 51– 56.
82. Khamis, A.,, D. Raoult,, and B. La Scola. 2004. rpoB gene sequencing for identification of Corynebacterium species. J. Clin. Microbiol. 42: 3925– 3931.
83. Khamis, A.,, D. Raoult,, and B. La Scola. 2005. Comparison between rpoB and 16S rRNA gene sequencing for molecular identification of 168 clinical isolates of Corynebacterium. J. Clin. Microbiol. 43: 1934– 1936.
84. Koch, C.,, F. A. Rainey,, and E. Stackebrandt. 1994. 16S rDNA studies on members of Arthrobacter and Micrococcus: an aid for their future taxonomic restructuring. FEMS Microbiol. Lett. 123: 167– 172.
85. Kronvall, G.,, M. Lanner-Sjoberg,, L. V. von Stedingk,, H. S. Hanson,, B. Pettersson,, and E. Falsen. 1998. Whole cell protein and partial 16S rRNA gene sequence analysis suggest the existence of a second Rothia species. Clin. Microbiol. Infect. 4: 255– 263.
86. Laffineur, K.,, V. Avesani,, G. Cornu,, J. Charlier,, M. Janssens,, G. Wauters,, and M. Delmee. 2003. Bacteremia due to a novel Microbacterium species in a patient with leukemia and description of Microbacterium paraoxydans sp. nov. J. Clin. Microbiol. 41: 2242– 2246.
87. Lagace-Wiens, P. R.,, B. Ng,, A. Reimer,, T. Burdz,, D. Wiebe,, and K. Bernard. 2008. Gardnerella vaginalis bacteremia in a previously healthy man: case report and characterization of the isolate. J. Clin. Microbiol. 46: 804– 806.
88. Lartigue, M. F.,, X. Monnet,, A. Le Flèche,, P. A. Grimont,, J. J. Benet,, A. Durrbach,, M. Fabre,, and P. Nordmann. 2005. Corynebacterium ulcerans in an immunocompromised patient with diphtheria and her dog. J. Clin. Microbiol. 43: 999– 1001.
89. Leonard, R. B.,, D. J. Nowowiejski,, J. J. Warren,, D. J. Finn,, and M. B. Coyle. 1994. Molecular evidence of person-toperson transmission of a pigmented strain of Corynebacterium striatum in intensive care units. J. Clin. Microbiol. 32: 164– 169.
90. Li, Y.,, Y. Kawamura,, N. Fujiwara,, T. Naka,, H. Liu,, X. Huang,, K. Kobayashi,, and T. Ezaki. 2004. Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir. Int. J. Syst. Evol. Microbiol. 54: 827– 835.
91. Loubinoux, J.,, B. Rio,, L. Mihaila,, E. Fois,, A. Le Flèche,, P. A. D. Grimont,, J.-P. Marie,, and A. Bouvet. 2005. Bacteremia caused by an undescribed species of Janibacter. J. Clin. Microbiol. 43: 3564– 3566.
92. Mages, I. S.,, R. Frodl,, K. A. Bernard,, and G. Funke. 2008. Identities of Arthrobacter spp. and Arthrobacter-like bacteria encountered in human clinical specimens. J. Clin. Microbiol. 46: 2980– 2986.
93. Manaia, C. M.,, B. Nogales,, N. Weiss,, and O. C. Nunes. 2004. Gulosibacter molinativorax gen. nov., sp. nov., a molinate degrading bacterium, and classification of “Brevibacterium helvolum” DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov. Int. J. Syst. Evol. Microbiol. 54: 783– 789.
94. Martin, K.,, P. Schumann,, F. A. Rainey,, B. Schuetze,, and I. Groth. 1997. Janibacter limosus gen. nov., sp. nov., a new actinomycete with meso-diaminopimelic acid in the cell wall. Int. J. Syst. Bacteriol. 47: 529– 534.
95. Martinez-Martinez, L.,, M. C. Ortega,, and A. I. Suarez. 1995. Comparison of E-test with broth microdilution and disk diffusion for susceptibility testing of coryneform bacteria. J. Clin. Microbiol. 33: 1318– 1321.
96. Merhej, V.,, E. Falsen,, D. Raoult,, and V. Roux. 2009. Corynebacterium timonense and Corynebacterium massiliense sp. nov., isolated from human blood and human articular hip liquid. Int. J. Syst. Evol. Microbiol. 59: 1953– 1959.
97. Miyake, T.,, K. Watanabe,, T. Watanabe,, and H. Oyaizu. 1998. Phylogenetic analysis of the genus Bifidobacterium and related genera based on 16S rDNA sequences. Microbiol. Immun. 42: 661– 667.
98. Mokrousov, I.,, O. Narvskaya,, E. Limeschenko,, and A. Vyazovaya. 2005. Efficient discrimination within a Corynebacterium diphtheriae epidemic clonal group by a novel macroarray- based method. J. Clin. Microbiol. 43: 1662– 1668.
99. Mothershed, E. A.,, P. K. Cassiday,, K. Pierson,, L. W. Mayer,, and T. Popovic. 2002. Development of a real-time fluorescence PCR assay for rapid detection of the diphtheria toxin gene. J. Clin. Microbiol. 40: 4713– 4719.
100. Nakao, H.,, I. K. Mazarova,, T. Glushkevich,, and T. Popovic. 1997. Analysis of heterogeneity of Corynebacterium diphtheriae toxin gene, tox, and its regulatory element, dtxR, by direct sequencing. Res. Microbiol. 148: 45– 54.
101. Nakao, H.,, and T. Popovic. 1997. Development of a direct PCR assay for detection of the diphtheria toxin gene. J. Clin. Microbiol. 35: 1651– 1655.
102. Neal, S. E.,, A. Efstratiou, on behalf of DIPNET and international diphtheria reference laboratories. 2010. International external quality assurance for the laboratory diagnosis of diphtheria. J. Clin. Microbiol. 47: 4037– 4042.
103. Neubauer, M.,, J. Sourek,, M. Ryc,, J. Bohacek,, M. Mara,, and J. Mnukova. 1991. Corynebacterium accolens sp. nov., a gram-positive rod exhibiting satellitism, from clinical material. Syst. Appl. Microbiol. 14: 46– 51.
104. Nugent, R. P.,, M. A. Krohn,, and S. L. Hillier. 1991. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of Gram stain interpretation. J. Clin. Microbiol. 29: 297– 301.
105. Ohtaki, H.,, K. Ohkusu,, H. Sawamura,, H. Ohta,, R. Inoue,, J. Iwasa,, H. Ito,, N. Murakami,, T. Ezaki,, H. Moriwaki,, and M. Seishima. 2009. First case report of acute cholecystitis with sepsis caused by Cellulomonas denverensis. J. Clin. Microbiol. 47: 3391– 3393.
106. Otsuka, Y.,, Y. Kawamura,, T. Koyama,, H. Iihara,, K. Ohkusu,, and T. Ezaki. 2005. Corynebacterium resistens sp. nov., a new multidrug-resistant coryneform bacterium isolated from human infections. J. Clin. Microbiol. 43: 3713– 3717.
107. Pallen, M. J.,, A. J. Hay,, L. H. Puckey,, and A. Efstratiou. 1994. Polymerase chain reaction for screening clinical isolates of corynebacteria for the production of diphtheria toxin. J. Clin. Pathol. 47: 353– 356.
108. Pascual, C.,, P. A. Lawson,, J. A. E. Farrow,, M. Navarro Gimenez,, and M. D. Collins. 1995. Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 45: 724– 728.
109. Pascual Ramos, C.,, G. Foster,, and M. D. Collins. 1997. Phylogenetic analysis of the genus Actinomyces based on 16S rRNA gene sequences: description of Arcanobacterium phocae sp. nov., Arcanobacterium bernardiae comb. nov., and Arcanobacterium pyogenes comb. nov. Int. J. Syst. Bacteriol. 47: 46– 53.
110. Paviour, S.,, S. Musaad,, S. Roberts,, G. Taylor,, S. Taylor,, K. Shore,, S. Lang,, and D. Holland. 2002. Corynebacterium species isolated from patients with mastitis. Clin. Infect. Dis. 35: 1434– 1440.
111. Pimenta, F. P.,, G. A. Matias,, G. A. Pereira,, T. C. Camello,, G. B. Alves,, A. C. Rosa,, R. Hirata, Jr., and A. L. Mattos-Guaraldi. 2008. A PCR for dtxR gene: application to diagnosis of non-toxigenic and toxigenic Corynebacterium diphtheriae. Mol. Cell. Probes 22: 189– 192.
112. Pitt, T. L.,, H. Malnick,, J. Shah,, M. A. Chattaway,, C. J. Keys,, F. J. Cooke,, and H. N. Shah. 2007. Characterisation of Exiguobacterium aurantiacum isolates from blood cultures of six patients. Clin. Microbiol. Infect. 13: 946– 948.
113. Popovic, T.,, I. K. Mazurova,, A. Efstratiou,, J. Vuopio- Varkila,, M. Reeves,, A. De Zoysa,, T. Glushkevich,, and P. A. D. Grimont. 2000. Molecular epidemiology of diphtheria. J. Infect. Dis. 181: S168– S177.
114. Rainey, F. A.,, N. Weiss,, H. Prauser,, and E. Stackebrandt. 1994. Further evidence for the phylogenetic coherence of actinomycetes with group B-peptidoglycan and evidence for the phylogenetic intermixing of the genera Microbacterium and Aureobacterium as determined by 16S rDNA analysis. FEMS Microbiol. Lett. 118: 135– 140.
115. Rassoulian-Barrett, S. L.,, B. T. Cookson,, L. C. Carlson,, K. A. Bernard,, and M. B. Coyle. 2001. Diversity within reference strains of Corynebacterium matruchotii includes Corynebacterium durum and a novel organism. J. Clin. Microbiol. 39: 943– 948.
116. Reacher, M.,, M. Ramsay,, J. White,, A. De Zoysa,, A. Efstratiou,, G. Mann,, A. Mackay,, and R. C. George. 2000. Nontoxigenic Corynebacterium diphtheriae: an emerging pathogen in England and Wales? Emerg. Infect. Dis. 6: 640– 645.
117. Renaud, F. N.,, D. Aubel,, P. Riegel,, H. Meugnier,, and C. Bollet. 2001. Corynebacterium freneyi sp. nov., alpha-glucosidase- positive strains related to Corynebacterium xerosis. Int. J. Syst. Evol. Microbiol. 51: 1723– 1728.
118. Renaud, F. N. R.,, A. Gregory,, C. Barreau,, D. Aubel,, and J. Freney. 1996. Identification of Turicella otitidis isolated from a patient with otorrhea associated with surgery: differentiation from Corynebacterium afermentans and Corynebacterium auris. J. Clin. Microbiol. 34: 2625– 2627.
119. Renaud, F. N. R.,, A. L. Coustumier,, N. Wilhem,, D. Aubel,, P. Riegel,, C. Bollet,, and J. Freney. 2007. Corynebacterium hansenii sp. nov., an alpha-glucosidase-negative bacterium related to Corynebacterium xerosis. Int. J. Syst. Evol. Microbiol. 57: 1113– 1116.
120. Rennie, R. P.,, C. Brosnikoff,, L. Turnbull,, L. B. Reller,, S. Mirrett,, W. Janda,, K. Ristow,, and A. Krilcich. 2008. Multicenter evaluation of the Vitek 2 anaerobe and Corynebacterium identification card. J. Clin. Microbiol. 46: 2646– 2651.
121. Renom, F.,, M. Garau,, M. Rubi,, F. Ramis,, A. Galmes,, and J. B. Soriano. 2007. Nosocomial outbreak of Corynebacterium striatum infection in patients with chronic obstructive pulmonary disease. J. Clin. Microbiol. 45: 2064– 2067.
122. Renvoise, A.,, N. Aldrovandi,, D. Raoult,, and V. Roux. 2009. Helcobacillus massiliensis gen. nov., sp. nov., a novel representative of the family Dermabacteraceae isolated from a patient with a cutaneous discharge. Int. J. Syst. Evol. Microbiol. 59: 2346– 2351.
123. Riegel, P.,, R. Creti,, R. Mattei,, A. Nieri,, and C. von Hunolstein. 2006. Isolation of Corynebacterium tuscaniae sp. nov. from blood cultures of a patient suffering from endocarditis. J. Clin. Microbiol. 44: 307– 312.
124. Riegel, P.,, D. de Briel,, G. Prevost,, F. Jehl,, and H. Monteil. 1994. G enomic diversity among Corynebacterium jeikeium strains and comparison with biochemical characteristics. J. Clin. Microbiol. 32: 1860– 1865.
125. Riegel, P.,, D. de Briel,, G. Prevost,, F. Jehl,, H. Monteil,, and R. Minck. 1993. Taxonomic study of Corynebacterium group ANF-1 strains: proposal of Corynebacterium afermentans sp. nov. containing the subspecies C. afermentans subsp. afermentans subsp. nov. and C. afermentans subsp. lipophilum subsp. nov. Int. J. Syst. Bacteriol. 43: 287– 292.
126. Riegel, P.,, R. Heller,, G. Prevost,, F. Jehl,, and H. Monteil. 1997. Corynebacterium durum sp. nov., from human clinical specimens. Int. J. Syst. Bacteriol. 47: 1107– 1111.
127. Riegel, P.,, R. Ruimy,, D. de Briel,, G. Prevost,, F. Jehl,, F. Bimet,, R. Christen,, and H. Monteil. 1995. Corynebacterium argentoratense sp. nov., from human throat. Int. J. Syst. Bacteriol. 45: 533– 537.
128. Riegel, P.,, R. Ruimy,, D. de Briel,, G. Prevost,, F. Jehl,, R. Christen,, and H. Monteil. 1995. Genomic diversity and phylogenetic relationships among lipid-requiring diphtheroids from humans and characterization of Corynebacterium macginleyi sp. nov. Int. J. Syst. Bacteriol. 45: 128– 133.
129. Riegel, P.,, R. Ruimy,, D. de Briel,, G. Prevost,, F. Jehl,, R. Christen,, and H. Monteil. 1995. Taxonomy of Corynebacterium diphtheriae and related taxa, with the recognition of Corynebacterium ulcerans sp. nov., nom. rev. FEMS Microbiol. Lett. 126: 271– 276.
130. Riegel, P.,, R. Ruimy,, F. N. R. Renaud,, J. Freney,, G. Prevost,, F. Jehl,, R. Christen,, and H. Monteil. 1997. Corynebacterium singulare sp. nov., a new species for ureasepositive strains related to Corynebacterium minutissimum. Int. J. Syst. Bacteriol. 47: 1092– 1096.
131. Romney, M. G.,, D. L. Roscoe,, K. Bernard,, S. Lai,, A. Efstratiou,, and A. M. Clarke. 2006. Emergence of an invasive clone of nontoxigenic Corynebacterium diphtheriae in the urban poor population of Vancouver, Canada. J. Clin. Microbiol. 44: 1625– 1629.
132. Roux, V.,, M. Drancourt,, A. Stein,, P. Riegel,, D. Raoult,, and B. La Scola. 2004. Corynebacterium species isolated from bone and joint infections identified by 16S rRNA gene sequence analysis. J. Clin. Microbiol. 42: 2231– 2233.
133. Ruimy, R.,, P. Riegel,, P. Boiron,, H. Monteil,, and R. Christen. 1995. Phylogeny of the genus Corynebacterium deduced from analysis of small-subunit ribosomal DNA sequences. Int. J. Syst. Bacteriol. 45: 740– 746.
134. Sadhu, K.,, P. A. G. Domingue,, A. W. Chow,, J. Nelligan,, N. Cheng,, and J. W. Costerton. 1989. Gardnerella vaginalis has a gram-positive cell-wall ultrastructure and lacks classical cell-wall lipopolysaccharide. J. Med. Microbiol. 29: 229– 235.
135. Schuhegger, R.,, R. Kugler,, and A. Sing. 2008. Pitfalls with diphtheria-like illness due to toxigenic Corynebacterium ulcerans. Clin. Infect. Dis. 47: 288.
136. Schuhegger, R.,, M. Lindermayer,, R. Kugler,, J. Heesemann,, U. Busch,, and A. Sing. 2008. Detection of toxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans strains by a novel real-time PCR. J. Clin. Microbiol. 46: 2822– 2823.
137. Schumann, P.,, N. Weiss,, and E. Stackebrandt. 2001. Reclassification of Cellulomonas cellulans (Stackebrandt and Keddie 1986) as Cellulosimicrobium cellulans gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 51: 1007– 1010.
138. Sing, A.,, M. Hogardt,, S. Bierschenk,, and J. Heesemann. 2003. Detection of differences in the nucleotide and amino acid sequences of diphtheria toxin from Corynebacterium diphtheriae and Corynebacterium ulcerans causing extrapharyngeal infections. J. Clin. Microbiol. 41: 4848– 4851.
139. Sjödén, B.,, G. Funke,, A. Izquierdo,, E. Akervall,, and M. D. Collins. 1998. Description of some coryneform bacteria isolated from human clinical specimens as Corynebacterium falsenii sp. nov. Int. J. Syst. Bacteriol. 48: 69– 74.
140. Soriano, F.,, J. M. Aguado,, C. Ponte,, R. Fernandez-Roblas,, and J. L. Rodriguez-Tudela. 1990. Urinary tract infection caused by Corynebacterium group D2: report of 82 cases and review. Rev. Infect. Dis. 12: 1019– 1034.
141. Spiegel, C. A. 1991. Bacterial vaginosis. Clin. Microbiol. Rev. 4: 485– 502.
142. Spiegel, C. A. 1999. Bacterial vaginosis: changes in laboratory practice. Clin. Microbiol. Newsl. 21: 33– 37.
143. Stackebrandt, E.,, S. Breymann,, U. Steiner,, H. Prauser,, N. Weiss,, and P. Schumann. 2002. Re-evaluation of the status of the genus Oerskovia, reclassification of Promicromonospora enterophila (Jager et al. 1983) as Oerskovia enterophila comb. nov. and description of Oerskovia jenensis sp. nov. and Oerskovia paurometabola sp. nov. Int. J. Syst. Evol. Microbiol. 52: 1105– 1111.
144. Stackebrandt, E.,, F. A. Rainey,, and N. L. Ward-Rainey. 1997. Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int. J. Syst. Bacteriol. 47: 479– 491.
145. Takeuchi, M.,, and K. Hatano. 1998. Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int. J. Syst. Bacteriol. 48: 739– 747.
146. Tanner, M. A.,, D. Shoskes,, A. Shahed,, and N. R. Pace. 1999. Prevalence of corynebacterial 16S rRNA sequences in patients with bacterial and “nonbacterial” prostatitis. J. Clin. Microbiol. 37: 1863– 1870.
148. Tauch, A.,, O. Kaiser,, T. Hain,, A. Goesmann,, B. Weisshaar,, A. Albersmeier,, T. Bekel,, N. Bischoff,, I. Brune,, T. Chakraborty,, J. Kalinowski,, F. Meyer,, O. Rupp,, S. Schneiker,, P. Viehoever,, and A. Pühler. 2005. Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora. J. Bacteriol. 187: 4671– 4682.
149. Tauch, A.,, J. Schneider,, R. Szczepanowski,, A. Tilker,, P. Viehoever,, K. H. Gartemann,, W. Arnold,, J. Blom,, K. Brinkrolf,, I. Brune,, S. Gotker,, B. Weisshaar,, A. Goesmann,, M. Droge,, and A. Pühler. 2008. Ultrafast pyrosequencing of Corynebacterium kroppenstedtii DSM44385 revealed insights into the physiology of a lipophilic corynebacterium that lacks mycolic acids. J. Biotechnol. 136: 22– 30.
150. Tauch, A.,, E. Trost,, A. Tilker,, U. Ludewig,, S. Schneiker,, A. Goesmann,, W. Arnold,, T. Bekel,, K. Brinkrolf,, I. Brune,, S. Gotker,, J. Kalinowski,, P. B. Kamp,, F. P. Lobo,, P. Viehoever,, B. Weisshaar,, F. Soriano,, M. Droge,, and A. Pühler. 2008. The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing. J. Biotechnol. 136: 11– 21.
151. Tong, J.,, C. Liu,, P. Summanen,, H. Xu,, and S. M. Finegold. 2010. Corynebacterium pyruviciproducens sp. nov., producing pyruvic acid. Int. J. Syst. Evol. Microbiol. 60: 1135– 1140.
152. Troxler, R.,, G. Funke,, A. von Graevenitz,, and I. Stock. 2001. Natural antibiotic susceptibility of recently established coryneform bacteria. Eur. J. Clin. Microbiol. Infect. Dis. 20: 315– 323.
153. Valero-Guillen, P. L.,, G. Yague,, and M. Segovia. 2005. Characterization of acyl-phosphatidylinositol from the opportunistic pathogen Corynebacterium amycolatum. Chem. Phys. Lipids 133: 17– 26.
154. Vaneechoutte, M.,, P. Riegel,, D. de Briel,, H. Monteil,, G. Verschraegen,, A. De Rouck,, and G. Claeys. 1995. Evaluation of the applicability of amplified rDNA-restriction analysis (ARDRA) to identification of species of the genus Corynebacterium. Res. Microbiol. 146: 633– 641.
155. Verhelst, R.,, H. Verstraelen,, G. Claeys,, G. Verschraegen,, J. Delanghe,, L. Van Simaey,, C. De Ganck,, M. Temmerman,, and M. Vaneechoutte. 2004. Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis. BMC Microbiol. 4: 16.
156. von Graevenitz, A. 2004. Rothia dentocariosa: taxonomy and differential diagnosis. Clin. Microbiol. Infect. 10: 399– 402.
157. von Graevenitz, A.,, and G. Funke. 1996. An identification scheme for rapidly and aerobically growing gram-positive rods. Zentral. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. 284: 246– 254.
158. vonGraevenitz, A.,, V. Pünter-Streit,, P. Riegel,, and G. Funke. 1998. Coryneform bacteria in throat cultures of healthy individuals. J. Clin. Microbiol. 36: 2087– 2088.
159. Wattiau, P.,, M. Janssens,, and G. Wauters. 2000. Corynebacterium simulans sp. nov., a non-lipophilic, fermentative Corynebacterium. Int. J. Syst. Evol. Microbiol. 50: 347– 353.
160. Wauters, G.,, J. Charlier,, M. Janssens,, and M. Delmee. 2000. Identification of Arthrobacter oxydans, Arthrobacter luteolus sp. nov., and Arthrobacter albus sp. nov., isolated from human clinical specimens. J. Clin. Microbiol. 38: 2412– 2415.
161. Wauters, G.,, B. van Bosterhaut,, M. Janssens,, and J. Verhaegen. 1998. Identification of Corynebacterium amycolatum and other nonlipophilic fermentative corynebacteria of human origin. J. Clin. Microbiol. 36: 1430– 1432.
162. Weiss, K.,, M. Laverdiere,, and R. Rivest. 1996. Comparison of antimicrobial susceptibilities of Corynebacterium species by broth microdilution and disk diffusion methods. Antimicrob. Agents Chemother. 40: 930– 933.
163. Yassin, A. F. 2007. Corynebacterium ureicelerivorans sp. nov., a lipophilic bacterium isolated from blood culture. Int. J. Syst. Evol. Microbiol. 57: 1200– 1203.
164. Yassin, A. F.,, and C. Siering. 2008. Corynebacterium sputi sp. nov., isolated from the sputum of a patient with pneumonia. Int. J. Syst. Evol. Microbiol. 58: 2876– 2879.
165. Yassin, A. F.,, U. Steiner,, and W. Ludwig. 2002. Corynebacterium appendicis sp. nov. Int. J. Syst. Evol. Microbiol. 52: 1165– 1169.
166. Yassin, A. F.,, U. Steiner,, and W. Ludwig. 2002. C orynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones ( 1983 ). Int. J. Syst. Evol. Microbiol. 52: 1001– 1005.
167. Zapardiel, J.,, E. Nieto,, M. I. Gegundez,, I. Gadea,, and F. Soriano. 1994. Problems in minimum inhibitory concentration determinations in coryneform organisms—comparison of an agar dilution and the Etest. Diagn. Microbiol. Infect. Dis. 19: 171– 173.
168. Zhi, X.,, W. Li,, and E. Stackebrandt. 2009. An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int. J. Syst. Evol. Microbiol. 59: 589– 608.
169. Zimmermann, O.,, C. Spröer,, R. M. Kroppenstedt,, E. Fuchs,, E. G. Köchel,, and G. Funke. 1998. Corynebacterium thomssenii sp. nov., a Corynebacterium with N-acetyl-bglucosaminidase activity from human clinical specimens. Int. J. Syst. Bacteriol. 48: 489– 494.
170. Zinkernagel, A. S.,, A. von Graevenitz,, and G. Funke. 1996. Heterogeneity within Corynebacterium minutissimum strains is explained by misidentified Corynebacterium amycolatum strains. Am. J. Clin. Pathol. 106: 378– 383.
a m-DAP, meso-diaminopimelic acid; LYS, lysine; ORN, ornithine; DAB, diaminobutyric acid.
b Exceptions: C. amycolatum, C. atypicum, and C. kroppenstedtii.
a Taxa shown without references are from our observations or from references 6 and 72 .
a Abbreviations and symbols: F, fermentation; O, oxidation; +, positive, –, negative, V, variable; ( ), delayed or weak reaction; ND, no data; REV, CAMP inhibition reaction; TBSA, tuberculostearic acid.
b Propionic acid as a glucose fermentation product.
c A blood culture isolate ( 8 ) was also O-nitrophenyl-β-d-galactopyranoside positive, oxidase positive, and weakly maltose positive but negative by the API Coryne system; propionic acid was not detected; β-galactosidase was not observed using two methods (API Coryne and API Zym systems); the API Coryne system code obtained is 0101104.
d C. diphtheriae biotype mitis is nitrate reductase positive, and C. diphtheriae biotype belfanti is nitrate reductase negative.
e C. simulans ( 159 ) is a strong nitrite reducer at low and high concentrations; nitrate reduction may appear to be negative unless further tested using zinc dust; one strain was catalase negative ( 8 ).
a Abbreviations and symbols: +, positive reaction; – negative reaction; v, variable reaction; O, oxidation; F, fermentation. See also references 6 , 72 , and 122 .

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 V. 
 V. 
 V. 
 V.