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CMV antigen-positive polymorphonuclear leukocytes. Note the nuclear staining when a monoclonal antibody directed against pp65 is used. (A) Magnification, x100; (B) magnification, x400.
Demonstration of CMV early antigens in the nuclei (arrows) of infected MRC-5 cells following shell vial culture and IFA staining. (A) Staining of immediate-early antigen appears as an even matte green fluorescence with specks of brighter green. (B) Viral inclusions (owl&apos;s eyes) may be visible in the nuclei. Magnification, x400.
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a Abbreviations: pol, polymerase; pp65, phosphoprotein 65; MIE, major immediate early; IE1, immediate early 1; ASR, analyte-specific reagent; RUO, research use only.

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