Source: https://people.epfl.ch/neeraj.dhar
Timestamp: 2019-04-21 22:55:15+00:00

Document:
My research is focused on studying bacterial persistence against antibiotics using genetic tools as well as novel single-cell technologies and methods based on microfluidics and timelapse fluorescence microscopy.
2007-present Senior Scientist, Laboratory of Microbiology and Microsystems, Global Health Institute, EPFL, Lausanne, Switzerland.
R. C. Sommer / S. Cole; A. C. Oates (Dir.) : Innovative Tools and in vivo Methods for Tuberculosis Drug Discovery and Development. Lausanne, EPFL, 2019. DOI : 10.5075/epfl-thesis-9248.
R. Sommer; J. Neres; J. Piton; N. Dhar; A. van der Sar et al. : Fluorescent Benzothiazinone Analogues Efficiently and Selectively Label Dpre1 in Mycobacteria and Actinobacteria; ACS Chemical Biology. 2018-11-01. DOI : 10.1021/acschembio.8b00790.
P. J. Soler Arnedo / S. Cole (Dir.) : Functional characterization of the virulence determinant ESX-1 from Mycobacterium tuberculosis. Lausanne, EPFL, 2018. DOI : 10.5075/epfl-thesis-9011.
K. E. Schneider / J. McKinney; N. Dhar (Dir.) : Single-cell division and killing dynamics of bacteria exposed to isocratic and time-dependent concentrations of antibiotic. Lausanne, EPFL, 2018. DOI : 10.5075/epfl-thesis-8585.
H. A. Eskandarian; P. D. Odermatt; J. X. Y. Ven; M. T. M. Hannebelle; A. P. Nievergelt et al. : Division site selection linked to inherited cell surface wave troughs in mycobacteria; Nature Microbiology. 2017. DOI : 10.1038/nmicrobiol.2017.94.
S. Senzani; D. Li; A. Bhaskar; C. Ealand; J. Chang et al. : An Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase is required for mycobacterial cell division; Scientific Reports. 2017. DOI : 10.1038/s41598-017-01184-7.
V. Singh; N. Dhar; J. Pato; G. S. Kolly; J. Kordulakova et al. : Identification of aminopyrimidine-sulfonamides as potent modulators of Wag31-mediated cell elongation in mycobacteria; Molecular Microbiology. 2017. DOI : 10.1111/mmi.13535.
V. Singh; S. Donini; A. Pacitto; C. Sala; R. C. Hartkoorn et al. : The Inosine Monophosphate Dehydrogenase, GuaB2, Is a Vulnerable New Bactericidal Drug Target for Tuberculosis; Acs Infectious Diseases. 2017. DOI : 10.1021/acsinfecdis.6b00102.
N. Dhar; J. Mckinney; G. Manina : Phenotypic Heterogeneity in Mycobacterium tuberculosis; Microbiology Spectrum. 2016. DOI : 10.1128/microbiolspec.TBTB2-0021-2016.
M. Martinez-Hoyos; E. Perez-Herran; G. Gulten; L. Encinas; D. Alvarez-Gomez et al. : Antitubercular drugs for an old target: GSK693 as a promising InhA direct inhibitor; Ebiomedicine. 2016. DOI : 10.1016/j.ebiom.2016.05.006.
S. M. Batt; M. Cacho Izquierdo; J. Castro Pichel; C. J. Stubbs; L. Vela-Glez Del Peral et al. : Whole Cell Target Engagement Identifies Novel Inhibitors of Mycobacterium tuberculosis Decaprenylphosphoryl-beta-D-ribose Oxidase; Acs Infectious Diseases. 2015. DOI : 10.1021/acsinfecdis.5b00065.
A. Vocat; R. C. Hartkoorn; B. Lechartier; M. Zhang; N. Dhar et al. : Bioluminescence for Assessing Drug Potency against Nonreplicating Mycobacterium tuberculosis; Antimicrobial Agents And Chemotherapy. 2015. DOI : 10.1128/Aac.00528-15.
J. Rullas; N. Dhar; J. D. Mckinney; A. Garcia-Perez; J. Lelievre et al. : Combinations of beta-Lactam Antibiotics Currently in Clinical Trials Are Efficacious in a DHP-I-Deficient Mouse Model of Tuberculosis Infection; Antimicrobial Agents And Chemotherapy. 2015. DOI : 10.1128/Aac.01063-15.
N. Dhar; V. Dubee; L. Ballell; G. Cuinet; J.-E. Hugonnet et al. : Rapid Cytolysis of Mycobacterium tuberculosis by Faropenem, an Orally Bioavailable beta-Lactam Antibiotic; Antimicrobial Agents And Chemotherapy. 2015. DOI : 10.1128/Aac.03461-14.
J. Neres; R. C. Hartkoorn; L. R. Chiarelli; R. Gadupudi; M. R. Pasca et al. : 2-Carboxyquinoxalines Kill Mycobacterium tuberculosis through Noncovalent Inhibition of DprE1; Acs Chemical Biology. 2015. DOI : 10.1021/cb5007163.
J. Vaubourgeix; G. Lin; N. Dhar; N. Chenouard; X. Jiang et al. : Stressed Mycobacteria Use the Chaperone ClpB to Sequester Irreversibly Oxidized Proteins Asymmetrically Within and Between Cells; Cell Host & Microbe. 2015. DOI : 10.1016/j.chom.2014.12.008.
G. Manina; N. Dhar; J. D. Mckinney : Stress and Host Immunity Amplify Mycobacterium tuberculosis Phenotypic Heterogeneity and Induce Nongrowing Metabolically Active Forms; Cell Host & Microbe. 2015. DOI : 10.1016/j.chom.2014.11.016.
S. Sharma; E. Gelman; C. Narayan; D. Bhattacharjee; V. Achar et al. : Simple and Rapid Method To Determine Antimycobacterial Potency of Compounds by Using Autoluminescent Mycobacterium tuberculosis; Antimicrobial Agents And Chemotherapy. 2014. DOI : 10.1128/Aac.03205-14.
F. Boldrin; M. Ventura; G. Degiacomi; S. Ravishankar; C. Sala et al. : The Phosphatidyl-myo-Inositol Mannosyltransferase PimA Is Essential for Mycobacterium tuberculosis Growth In Vitro and In Vivo (vol 196, pg 3441, 2014); Journal Of Bacteriology. 2014. DOI : 10.1128/Jb.02332-14.
M. Zhang; J. M. Chen; C. Sala; J. Rybniker; N. Dhar et al. : EspI regulates the ESX-1 secretion system in response to ATP levels in Mycobacterium tuberculosis; Molecular Microbiology. 2014. DOI : 10.1111/mmi.12718.
F. Boldrin; M. Ventura; G. Degiacomi; S. Ravishankar; C. Sala et al. : The Phosphatidyl-myo-Inositol Mannosyltransferase PimA Is Essential for Mycobacterium tuberculosis Growth In Vitro and In Vivo; Journal Of Bacteriology. 2014. DOI : 10.1128/Jb.01346-13.
M. Zhang; C. Sala; N. Dhar; A. Vocat; V. K. Sambandamurthy et al. : In Vitro and In Vivo Activities of Three Oxazolidinones against Nonreplicating Mycobacterium tuberculosis; Antimicrobial Agents And Chemotherapy. 2014. DOI : 10.1128/Aac.02410-14.
M. Naik; V. Humnabadkar; S. J. Tantry; M. Panda; A. Narayan et al. : 4-Aminoquinolone Piperidine Amides: Noncovalent Inhibitors of DprE1 with Long Residence Time and Potent Antimycobacterial Activity; Journal Of Medicinal Chemistry. 2014. DOI : 10.1021/jm5005978.
G. S. Kolly; F. Boldrin; C. Sala; N. Dhar; R. C. Hartkoorn et al. : Assessing the essentiality of the decaprenyl-phospho-D-arabinofuranose pathway in Mycobacterium tuberculosis using conditional mutants; Molecular Microbiology. 2014. DOI : 10.1111/mmi.12546.
A. Koul; L. Vranckx; N. Dhar; H. W. H. Gohlmann; M. E. Özdemir et al. : Delayed bactericidal response of Mycobacterium tuberculosis to bedaquiline involves remodelling of bacterial metabolism; Nature Communications. 2014. DOI : 10.1038/ncomms4369.
E. Gelman / J. McKinney; N. Dhar (Dir.) : Antibiotic-Induced Killing and Persistence of Mycobacteria. Lausanne, EPFL, 2014. DOI : 10.5075/epfl-thesis-6056.
J. M. Chen; M. Zhang; J. Rybniker; L. Basterra; N. Dhar et al. : Phenotypic Profiling of Mycobacterium tuberculosis EspA Point Mutants Reveals that Blockage of ESAT-6 and CFP-10 Secretion In Vitro Does Not Always Correlate with Attenuation of Virulence; Journal Of Bacteriology. 2013. DOI : 10.1128/Jb.00967-13.
J. M. Chen; M. Zhang; J. Rybniker; S. Boy-Röttger; N. Dhar et al. : Mycobacterium tuberculosis EspB binds phospholipids and mediates EsxA-independent virulence; Molecular Microbiology. 2013. DOI : 10.1111/mmi.12336.
I. Santi; N. Dhar; D. Bousbaine; Y. Wakamoto; J. D. McKinney : Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria; Nature Communications. 2013. DOI : 10.1038/ncomms3470.
Y. Wakamoto; N. Dhar; R. Chait; K. E. Schneider; F. Signorino-Gelo et al. : Dynamic Persistence of Antibiotic-Stressed Mycobacteria; Science. 2013. DOI : 10.1126/science.1229858.
J. Neres; F. Pojer; E. Molteni; L. R. Chiarelli; N. Dhar et al. : Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosis; Science Translational Medicine. 2012. DOI : 10.1126/scitranslmed.3004395.
M. Zhang; C. Sala; R. C. Hartkoorn; N. Dhar; A. Mendoza-Losana et al. : Streptomycin-Starved Mycobacterium tuberculosis 18b, a Drug Discovery Tool for Latent Tuberculosis; Antimicrobial Agents And Chemotherapy. 2012. DOI : 10.1128/Aac.01125-12.
E. Gelman; J. D. McKinney; N. Dhar : Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria; Antimicrobial Agents And Chemotherapy. 2012. DOI : 10.1128/AAC.00406-12.
J. M. Chen; S. Boy-Röttger; N. Dhar; N. Sweeney; R. S. Buxton et al. : EspD Is Critical for the Virulence-Mediating ESX-1 Secretion System in Mycobacterium tuberculosis; Journal of bacteriology. 2011. DOI : 10.1128/JB.06417-11.
M. Ballester; C. Nembrini; N. Dhar; A. de Titta; C. de Piano et al. : Nanoparticle conjugation and pulmonary delivery enhance the protective efficacy of Ag85B and CpG against tuberculosis; Vaccine. 2011. DOI : 10.1016/j.vaccine.2011.07.039.
F. Boldrin; S. Casonato; E. Dainese; C. Sala; N. Dhar et al. : Development of a repressible mycobacterial promoter system based on two transcriptional repressors; Nucleic acids research. 2010. DOI : 10.1093/nar/gkq235.
C. Sala; N. Dhar; R. C. Hartkoorn; M. Zhang; Y. H. Ha et al. : A Simple Model for Testing Drugs against Non-replicating Mycobacterium tuberculosis; Antimicrobial agents and chemotherapy. 2010. DOI : 10.1128/AAC.00821-10.
N. Dhar; J. D. McKinney : Mycobacterium tuberculosis persistence mutants identified by screening in isoniazid-treated mice; Proceedings of the National Academy of Sciences of the United States of America. 2010. DOI : 10.1073/pnas.1003219107.
B. Dey; R. Jain; A. Khera; V. Rao; N. Dhar et al. : Boosting with a DNA vaccine expressing ESAT-6 (DNAE6) obliterates the protection imparted by recombinant BCG (rBCGE6) against aerosol Mycobacterium tuberculosis infection in guinea pigs; Vaccine. 2009. DOI : 10.1016/j.vaccine.2009.09.121.
V. Makarov; G. Manina; K. Mikusova; U. Möllmann; O. Ryabova et al. : Benzothiazinones kill Mycobacterium tuberculosis by blocking arabinan synthesis; Science (New York, N.Y.). 2009. DOI : 10.1126/science.1171583.
R. Jain; B. Dey; N. Dhar; V. Rao; R. Singh et al. : Enhanced and Enduring Protection against Tuberculosis by Recombinant BCG-Ag85C and Its Association with Modulation of Cytokine Profile in Lung; Plos One. 2008. DOI : 10.1371/journal.pone.0003869.
N. Dhar; J. D. McKinney : Microbial phenotypic heterogeneity and antibiotic tolerance; Current opinion in microbiology. 2007. DOI : 10.1016/j.mib.2006.12.007.

References: V. 

V. 
 V. 
 V. 
 V. 
 V. 
 V. 

V. 
 V.