Source: https://ichbe.sgu.ru/en/articles/the-effect-of-pollutants-on-the-intensity-of-biofilm-formation-by-the-strain-vibrio
Timestamp: 2019-04-20 10:42:50+00:00

Document:
Most microorganisms in natural conditions exist in the form of biofilms. Due to this fact, the question of research of various substances influence on their formation process is relevant. This article presents data on the influence of various groups of pollutants, such as antibiotics (ampicillin, oxytetracycline), antiseptics (dioxidine, chlorhexidine), herbicides (glyphosate, clopyralid) and salts of heavy metals (CuSO4, HgCl2) in various concentrations on the intensity of biofilm formation by the strain Vibrio aquamarinus VKPM B-11245. To determine the intensity of biofilm formation the method of staining by crystal violet was used. It is shown that within each group of the investigated substances, the individual substances have different effects on biofilms formation. HgCl2 in the concentration of 0.27 μg/ml has the maximum stimulating effect, and the maximum inhibitory effect was displayed by oxytetracycline (2.5 μg/ml and 25 μg/ml) and dioxidine (100 μg/ml and 1000 μg/ml), as in these concentrations biofilm formation was not detected.
1. Rabin N., Zheng Y., Opoku-Temeng C., Du Y., Bonsu E., Sintim H. O. Biofi lm formation mechanisms and targets for developing antibiofi lm agents. Future, 2015, vol. 7, no. 4, pp. 493‒512.
2. Rittmann B.E. Biofi lms, active substrata, and me. Water Research, 2018, vol. 132, pp. 135‒145.
3. Goldberg J. Biofi lms and antibiotic resistance: a genetic linkage. TRENDS in Microbiology, 2002, vol. 10, no. 6, pp. 264.
4. Peng J. S., Tsai W. C., Chou C. C. Inactivation and removal of Bacillus cereus by sanitizer and detergent. International Journal of Food Microbiology, 2002, vol. 77, no. 1‒2, pp. 11‒18.
5. Hobley L., Harkins C., MacPhee C. E., Stanley-Wall N. R. Giving structure to the biofi lm matrix: an overview of individual strategies and emerging common themes. FEMS Microbiology Reviews, 2015, vol. 39, no. 5, pp. 649‒669.
6. Sekoulov I., Brinke-Seiferth S. Application of biofi ltration in the crude oil processing industry. Water science and technology, 1999, vol. 39, no. 8, pp. 71‒76.
7. Garrett T.R., Bhakoo M., Zhang Z. Bacterial adhesion and biofi lms on surfaces. Progress in Natural Science, 2008, vol. 18, no. 9, pp. 1049‒1056.
8. Jamal M., Ahmad W., Andleeb S., Jalil F., Imran M., Nawaz M.A., Hussain T., Ali M., Rafi c M., Kamil M. A. Bacterial biofi lm and associated infections. Journal of the Chinese Medical Association, 2018, vol. 81, no. 1, pp. 7‒11.
9. Petruhina M. I., Yushchenko G. V., Politova N. G. Epidemiologicheskoe znachenie bakterial’nyh plyonok [Epidemilogical value of bacterial slimes]. Zhurnal MediAl’ [Journal MediAl’], 2015, no. 3 (17), pp. 9‒17 (in Russian).
10. Stepanović S., Vuković D., Dakić I., Savić B., Švabić-Vlahović M. A modifi ed microtiter-plate test for quantifi cation of staphylococcal biofi lm formation. Journal of Microbiological Methods, 2000, vol. 40, no. 2, pp. 175‒179.
11. Sambrook J., Fritsch E. F., Maniatis T. Molecular cloning: A Laboratory Manual. Cold Spring Habor Laboratory Press, 1982. 479 p.
12. Manual of methods for general bacteriology / by Ph. Gerhardt, Editor-in-chief R. G. E. Murray, editor I. Morphology ... [et al.]. Washington, D.C.: American Society for Microbiology, 1981. 524 p.
13. Lakin G. F. Biometriya [Biometry]. Moscow, Vyssh. Shk. Publ., 1990. 351 p.
14. Chambless J. D., Hunt S. M., Stewart P. S. A threedimensional computer model of four hypothetical mechanisms protecting biofi lms from antimicrobials. Applied and Environmental Microbiology, 2006, vol. 72, no. 3, pp. 2005‒2013.
15. Zheldakova R. A. Mekhanizmy biosinteza antibiotikov i ih dejstvie na kletki mikroorganizmov [Mechanisms of biosynthesis of antibiotics and their effect on microorganisms cells]. Minsk, BGU, 2004. 111 p.
16. Pirueva T. A. Ocenka antisepticheskogo vozdejstviya na vozbuditelej hirurgicheskih infekcij v sostave bioplenok [Assessment of antiseptic effects on pathogens of surgical infections in the composition of biofi lms]. Vserossijskaya Itogovaya 74-ya studencheskaya nauchnaya konferenciya im. N. I. Pirogova (Tomsk, 27‒29 aprelya 2015 g.): sb. materialov. Ed. G. E. Chernogoryuka. Tomsk, SGMU, 2015, pp. 97–98 (in Russian).
17. Registr lekarstvennyh sredstv Rossii (Register of medicinal preparations of Russia). Available at: https://www.rlsnet.ru/mnn_index_id_142.htm (accessed 14 March 2018) (in Russian).
18. Kuznetsova E. M., Chmil V. D. Glifosat: povedenie v okruzhayushchej srede i urovni ostatkov [Glyphosate: environmental fate and levels of residues]. Sovremennye problemy toksikologii [Modern Problems of Toxicology], 2010, no. 1, pp. 87‒95 (in Russian).
19. Miroshnikova D. I., Motalova T. V. Toksikologo-gigienicheskaya harakteristika pesticidov na osnove glifosata [Toxicological and hygienic characteristics of glyphosate-based pesticides]. Materialy ezhegodnoj nauchnoj konferencii Ryazanskogo gos. medicinskogo un-ta im. akademika I. P. Pavlova [Materials of the Annual. Sci. Conf. of the Ryazan State Medical University named after acad. I. P. Pavlova]. Ryazan’, RIO RyazGMU, 2016, pp. 313‒316 (in Russian).
20. Gosudarstvennyj katalog pesticidov i agrohimikatov, razreshennyh k primeneniyu na territorii Rossijskoj Federacii [State catalogue of pesticides and agrochemicals permitted for use on the territory of the Russian Federation]. Moscow, Minsel’hoz RF, 2017. 938 p. (in Russian).
21. Sokolov E. М., Panarin V. M., Ryleyeva E. M. Antropogennoe zagryaznenie okruzhayushchej sredy tyazhelymi metallami [The Universal Device for Sewage Purifi cation with Closed System of Water Rotation]. Ekologiya i promyshlennost’ Rossii [Ecology and Industry of Russia], 2008, vol. 11, pp. 4‒6 (in Russian).
22. Sazykina M. A., Chistyakov V. A., Sazykin I. S., Lagutova L. P., Novikova E. M., Latyshev A. I. Ispol’zovanie bakterial’nogo lux-biosensora dlya detekcii zagryazneniya prirodnyh vod rtut’yu [Bacterial lux-biosensor for detection of mercury pollution]. Voda: himiya i ekologiya [Water: Chemistry and Ecology], 2010, no. 5, pp. 24‒29 (in Russian).
23. Ignatenko A. V. Izuchenie obrazovaniya bioplenok bakterij i ocenka ih ustojchivosti k biocidam [Bacteria biofi lms formation and estimation of their stability to biocides]. Trudy BGTU. Ser. 4: Himiya, tekhnologiya organicheskih veshchestv i biotekhnologiya [Proceeding of BSTU. Ser. 4. Chemistry, Organic Substances Texnology and Biotexnology], 2008, vol. 1, no. 4, pp. 173‒176 (in Russian).
24. Bonez P. C., dos Santos Alves C. F., Dalmolin T. V., Agertt V. A., Mizdal C. R., Flores Vda C, Marques J. B., Santos R. C., Anraku de Campos M. M. Chlorhexidine activity against bacterial biofi lms. American Journal of Infection Control, 2013, vol. 41, no. 12, pp. e119‒e122.

References: V. 
 V. 
 V. 
 V. 
 V. 
 V.