Source: https://pubs.rsc.org/en/content/articlehtml/2019/na/c8na00343b
Timestamp: 2019-04-20 01:23:29+00:00

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Silver nanoparticles (NPs) were synthesized using an efficient bioreducing agent from Fagonia cretica extract having the advantage of eco-friendliness over chemical and physical methods. The sharp color change and appearance of representative absorption peaks in the UV-visible spectra confirm the quick reduction of the Ag salt and evolution of Ag NPs. Morphological and structural aspects showed that the resulting Ag NPs are highly crystalline with an average size of 16 nm. Furthermore, compositional analysis of the extract confirmed the existence of active bioreducing and stabilizing agents in the Fagonia cretica extract. Furthermore, various concentrations of AgNO3 and the Fagonia cretica extract were employed to obtain a higher yield with better stability of Ag NPs. The resulting Ag NPs showed effective antibacterial activity against Proteus vulgaris, Escherichia coli, and Klebsiella pneumoniae. It is found that the Ag NPs induce maximum production of reactive oxygen species (ROS) in Proteus vulgaris as compared to Escherichia coli and Klebsiella pneumoniae which induce cell toxicity, while ROS production in the presence of Ag NPs is 30% higher than that in the presence of only the plant extract and control in all three bacterial strains. Thus, present findings show that plant extracts can be a useful natural resource to prepare functional nanomaterials for targeted applications especially in the field of biotechnology.
Considering the advantages of green synthesis over other methods, here, we have explored a new plant extract named Fagonia cretica as the reducing and stabilizing agent for the synthesis of Ag NPs due to its abundance and low cost as well as no involvement of any bacterial or fungal species like other biological synthesis methods which eliminates all associated clinical concerns which eliminates all associated clinical concerns, thus, making the Fagonia cretica extract a unique bioreducing source that is worth exploring for the mentioned purpose. The as-synthesized Ag NPs are highly crystalline and exhibited significant anti-microbial activities against various strains of Gram negative bacteria, confirming that biological reactors are a better option for the synthesis of nanomaterials for wide application in future not only in medical applications but also in environmental and energy applications.
A Fagonia cretica plant was obtained from the Agriculture Department of the Islamia University of Bahawalpur, Pakistan, the Herberium certificate of Fagonia cretica is given in the ESI for plant collection and habitat (Certificate 1 ESI †). After drying the pieces of the Fagonia cretica plant, they were ground to get a fine powder. Afterwards, 1.5 g of the Fagonia cretica powder was mixed with 50 mL of solutions containing different ethanol to water ratios (30, 50, 70 & 90% ethanol, as shown in Fig. S1†) in a 250 mL conical flask and shaken overnight at 37 °C. Finally, the extract was filtered using filter paper, and the filtrate was kept at 4 °C for further use.
Silver NPs synthesized using the bioreducing agents extracted from Fagonia cretica are of great interest because of their unique characteristics. The reduction of AgNO3 by the Fagonia cretica extract is first observed by a change in color from light orange to dark red indicating the strong absorption of visible light due to the excitation of the NP surface plasmons (the inset of Fig. 1a), which depends on the size, shape and concentration of the Ag NPs.20 Furthermore, to confirm the reduction of Ag ions to metallic Ag NPs, UV-vis spectra were recorded at various time intervals of 15, 30, 60 & 120 min where the maximum absorbance was observed at 440 nm (Fig. 1a). The control sample does not show any peak due to the absence of the reducing agent (plant extract) resulting in no formation of Ag NPs, while the sample having the plant extract shows maximum intensity for 60 & 120 min assuring that the Fagonia cretica extract plays a main role in reducing Ag ions to form Ag NPs. The size of the NPs is a matter of great concern specially for their biological applications as the size of NPs strongly affects the rate of diffusion through biological membranes. Previously, it has been observed that small size NPs have more penetration power, but too small a size brings the issue of enhanced toxicity compared to larger size NPs; thus, an appropriate size is highly desirable for specific biological applications.6,21 Therefore, transmission electron microscopy (TEM) was used to examine the morphology and size of NPs prior to their biological application. Fig. 1b clearly shows that the as-synthesized NPs are spherical in shape and have a very narrow size distribution where 70% of the NPs have a size in the range of 11–15 nm, while 22% have a size between 16 and 45 nm and only 1% have a size smaller than 5 nm (the inset of Fig. 1b). The abundant NPs with a size range of 11–15 nm are highly suitable for biomedical applications as the size of the synthesized NPs is within the tolerable range for inducing toxicity within cells.
Fig. 1 (a) UV-vis spectra of Ag NPs with the Fagonia cretica extract at different time points (the inset shows the colour change before and after Ag ion reduction) and (b) TEM image of Ag NPs, while the inset shows the particle size distribution.
It is very common that the concentrations of the salt precursor (AgNO3) and Fagonia cretica extract will have a large impact on the nuclei formation, growth and yield of Ag NPs. To explore the effect of AgNO3 and the Fagonia cretica extract, various concentrations were employed while keeping the other one constant. The concentration of AgNO3 strongly influences the formation of Ag NPs as a sharp absorption peak for Ag NPs was noticed at the 2.0 mM concentration of AgNO3 as compared to 0.5 mM and 1.0 mM as shown in Fig. 2a, which confirms that an optimal concentration of Ag+ ions is required as a deficiency of ions at a lower concentration and excessive ions at a higher concentration result in the formation of improper and/or complex intermediates that ultimately bring about poor conversion efficiencies. Similarly, a change in the concentration of the Fagonia cretica extract also influenced the growth of Ag NPs; at a low concentration of the extract, no peak was observed in the UV-vis spectrum confirming that low concentrations are unable to provide enough bioreducing molecules for formation of stable Ag+ ion complexes and their conversion to metallic Ag NPs (Fig. 2b). However, increasing the concentration of the Fagonia cretica extract from 10 to 20 mL results in a sharp peak confirming the formation of appropriate metal–organic complexes that later on yield Ag NPs as shown in Fig. 2b. However, with further increase in the concentration of the Fagonia cretica extract up to 40 mL, we found that a reduction in the absorption peak corresponds to the poor yield of Ag NPs and overall high absorbance due to the large quantity of the plant extract (organic molecules). In addition to the reduction in the absorption peak, a change in the peak position is also observed which might be due to the formation of improper metal–organic complexes in the presence of a higher concentration of organic molecules from the Fagonia cretica extract (Fig. 2b). Thus, this confirms that to obtain Ag NPs with better material features, appropriate concentrations of AgNO3 and the Fagonia cretica extract are highly required; however, antimicrobial activity was assessed using Ag NPs prepared under the best conditions.
Fig. 2 UV-vis absorption spectra of Ag NPs synthesized at different concentrations of (a) AgNO3 and (b) the plant extract.
To identify the role of the Fagonia cretica extract in the reduction of Ag ions, HPLC studies of the Fagonia cretica extract were conducted before using it as the reducing agent and after it reduced Ag ions to Ag NPs to confirm its role in reduction, and the results are shown in Fig. 3a. A clear difference in absorbance on the basis of retention time was observed before and after the reduction reaction (full spectra are shown in Fig. S2†). A change in the peak height of about 30% and disappearance of the shoulder peak at 2.793 min (pointed by black and red arrows in Fig. 3a) confirm that the Fagonia cretica extract is responsible for the reduction of Ag ions to Ag NPs. Hence, it is proved that the Fagonia cretica extract contains the respective chemical entities that are involved in reduction of Ag NPs and act as the capping agent for their stability and suitability for biomedical applications.
Fig. 3 (a) Chromatographic profiling of the Fagonia cretica extract before and after reduction and (b) XRD spectra of Ag NPs synthesized using the Fagonia cretica extract and chemical method.
The crystallinity of Ag NPs was characterized by X-ray diffraction (XRD) that delineates their face centered cubic (fcc) structure (Fig. 3b). The XRD spectrum of the biologically synthesized Ag NPs showed distinct peaks at 2θ of 38°, 45°, 65 and 77° corresponding to lattice planes of (111), (200), (220) and (311), respectively, in good agreement with standard PDF no. 04-0783 while that of the chemically synthesized Ag NPs showed diffraction peaks at 38°, 45° and 77°. However, the presence of more and sharp diffraction peaks confirms the better crystallinity of the biologically synthesized Ag NPs, showing that biological synthesis is an effective way not only for biological applications but also for many other fields as it helps to produce large amounts in easy ways with possibilities to create various functionalities. The Scherrer equation (D = kλ/β cos θ) is applied to determine the crystallite size of Ag particles which is 1.66 nm (biologically) and 1.19 nm (chemically); the smaller crystallite size might be due to the presence of capping agents in the Fagonia cretica extract that control the growth to a smaller size and provide high stability. Furthermore, Fig. S4† shows the stability of the colloidal solution of Ag NPs at pH 4, where it is clear that the suspension showed high stability even after one month. A slight change in color after one month shows little sedimentation of NPs; however, no obvious aggregation of Ag NPs was observed in the one-month old solution which demonstrates their high stability and suitability for biomedical applications.
Comparative antimicrobial activities of the as-synthesized Ag NPs and standard ciprofloxacin against strains of Proteus vulgaris, Escherichia coli and Klebsiella pneumonia have been investigated. These microbes cause infections mainly in people with an impaired immunity system, and most of them may be a source of complicated urinary tract, wound and nosocomial infections. Our results showed that the antimicrobial activity of the synthesized Ag NPs is significant against the growth of Proteus vulgaris, Escherichia coli and Klebsiella pneumoniae strains with different concentrations while the Fagonia cretica extract (tested to eliminate its impact and validity of Ag NPs) with all different concentrations has not shown any significant antimicrobial activity (Fig. 4a–c). It is found that growth of all three bacterial strains (Proteus vulgaris, Escherichia coli, and Klebsiella pneumoniae) was significantly suppressed by treating with Ag NPs and ciprofloxacin compared to control experiments (Tables S1–S3†). Furthermore, Ag NPs have shown maximum antimicrobial activity for Proteus vulgaris while they show minimum antimicrobial activity in Escherichia coli for all concentrations (5, 10 & 20 μg), and a similar trend is observed for the Fagonia cretica extract but with much lower activity as shown in Fig. 4d and e. Ciprofloxacin showed better antimicrobial activity against Escherichia coli and Klebsiella pneumoniae at different concentrations and comparatively lower activity against Proteus vulgaris (Fig. 4e). However, Ag NPs show much better antimicrobial activity for all three strains as compared to commercial ciprofloxacin and the Fagonia cretica extract that shows that the as-synthesized Ag NPs are more effective than commercially available ciprofloxacin.
Fig. 4 Antibacterial activity of (a) the synthesized Ag NPs, (b) ciprofloxacin and (c) the Fagonia cretica extract via the disc method. A plot of the concentration of (d) Ag NPs, (e) ciprofloxacin and (f) the Fagonia cretica extract vs. the zone of bacterial inhibition.
The oxidation of Ag NPs into Ag+ ions assist their diffusion into the biological system or put oxidative stress to produce reactive oxygen species (ROS) that change the permeability of membranes, damage DNA/RNA, proteins and lipids and finally induce cytotoxicity in prokaryotic cells.28,29 Therefore, investigation of the magnitude of ROS production in all three bacterial strains (Proteus vulgaris, Escherichia coli, and Klebsiella pneumoniae) in response to various concentrations (5, 10 & 20 μg) of Ag NPs, ciprofloxacin and the plant extract is very important to quantify the level of toxicity in comparison to control experiments, and the results are presented in Fig. 5a–c. The ROS generation was totally dependent on the dose of treatment as can be seen from Fig. 5a in which as the concentration of Ag NPs increased from 5 μg mL−1 to 20 μg mL−1 the production of ROS also increases to its maximum in all bacterial strains. However, ROS calculations have shown that Ag NPs are comparatively more effective towards Proteus vulgaris as compared to Escherichia coli and Klebsiella pneumoniae. On the other hand, a variation in the ciprofloxacin concentration did not influence the production of ROS much; however, ciprofloxacin shows maximum production of ROS in Escherichia coli as compared to Klebsiella pneumoniae and Proteus vulgaris as shown in Fig. 5b. The plant extract induces the production of a very small amount of ROS; therefore, its concentration did not induce much variation in ROS production and no specificity for any bacterial strain was found as shown in Fig. 5c.
Fig. 5 Production of ROS in various bacterial strains subject to different concentrations of (a) Ag NPs (b) ciprofloxacin and (c) the plant extract after 24 h exposure. (d) Schematic description of the possible mechanism of the action of the antibacterial activity of Ag NPs.
In conclusion, we have successfully synthesized Ag NPs using Fagonia cretica extract in ethanol which is highly environmentally benign. The morphological and structural analysis shows that the as-synthesized Ag NPs are highly crystalline with an average particle size of 16 nm. Furthermore, when employed as an antimicrobial agent, the crystalline Ag NPs showed significant antibacterial activity against Proteus vulgaris, Escherichia coli, and Klebsiella pneumoniae by producing 30% more ROS than the control. The HPLC and FTIR analysis revealed that the Fagonia cretica extract has the ability to reduce Ag ions to Ag atoms which construct Ag NPs and consequently coat them with hydroxyl and amine groups for high stability. Hence, easy and fast production of functional Ag NPs makes the Fagonia cretica extract a promising candidate for the preparation of nanomaterials for various applications e.g. in biomedicine, pharmacokinetics, pharmaceutics and bio-nanotechnology.
The authors would like to acknowledge the financial and technical support provided by the Islamia University of Bahawalpur, Pakistan, Peking University, China and RMIT University, Australia. The authors would also like to acknowledge the Vice-Chancellor Research Fellowship scheme at RMIT University for funding.
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