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Timestamp: 2019-04-26 05:41:20+00:00

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The chemical composition of the three essential oils obtained by steam distillation of the mastic gum, leaves and twigs of Pistacia lentiscus var. chia, was studied by GC/MS. Sixty nine constituents were identified from the oils. alpha-Pinene, myrcene, trans-caryophyllene and germacrene D were found to be the major components. The in vitro antimicrobial activity of the three essential oils and of the resin (total, acid and neutral fraction) against six bacteria and three fungi is reported.
The addition of mastic gum in broth culture inoculated with Staphylococcus aureus, Lactobacillus plantarum, Pseudomonas fragi and Salmonella enteritidis inhibited the growth of these organisms as monitored with the Malthus Instrument (in microsiemens). The rate of inhibition was greater on Gram positive bacteria than that observed on Gram negative bacteria. In most cases the size of inoculum and the concentration of mastic gum affected the growth/survival of the organisms. The addition of EDTA in coliform broth increased the inhibitory activity of mastic gum against Salmonella enteritidis, but did not affect significantly the inhibition of this organism in Model Food System (skim milk) at 37°C.
Iauk L, Ragusa S, Rapisarda A, Franco S, Nicolosi VM.
Studies were done to determine the effect of mastic resin and its essential oil, alone and in conjunction with ethanol, on the growth of proteolytic strains of Clostridium botulinum in media, and on neurotoxin production in challenge studies with English-style crumpets. Preliminary studies, using a spot-on-the-lawn method, indicated that high levels of mastic resin in ethanol (~8% w/w) were required for complete inhibition of all strains of C. botulinum tested, but mastic resin in ethanol had a greater anti-botulinal effect than ethanol alone. However, only low levels of mastic oil (~0.3% v/v) were required for inhibition of proteolytic strains of C. botulinum. Both studies showed a strain specific inhibition, with C. botulinum type A strains being more sensitive to mastic resin and its essential oil than type B strains. However, mastic resin in ethanol proved to be more effective when used as a vapor phase inhibitor applied to cotton pads and placed inside inoculated plates than when added directly to media. While both mastic resin and its essential oil inhibited the growth of proteolytic strains of C. botulinum in vitro, they failed to inhibit neurotoxin production in challenge studies with C. botulinum in English-style crumpets.
5. Epitheorese Klinikes Farmakologias kai Farmakokinetikes, International Edition. 2001 16: 199-201.
The Antibacterial and antifungal Action of the Chios Mastic Essential Oil.
E.E Protopapa, V Spiliotis, V. Kefala, A. Triantafyllou, J Tsaknis.
Technological Educational Institution, Athens & Laboratory og Biologikal Chemistry, University of Athens Medical School.
The effect of Chios mastic essential oil on the growth of nine bacteria (m. Luteus, S. epidermis, B. subtilis, S. aureus, E. Coli, S. Mutans, V. alcalencens, V. parulla and l. casei) and three fungi ( C. albicans, A. maelundi and A. odontolyticus) was tested in cultures in a minimal concentration inhibition assay. Bacteriostatic effects were observed on M. Luteus, S. epidermis, B. Subtilis, S. Aureus, E. Coli, and V. alcalencens and antifungal effects on C. albicans and A. maelundi. The bacteriostatic and antifungal effects of the mastic oil on A. maelundi and V. alcalencens, respectively, microorganisms which are present in the mouth flora, could be correlated with the observed effects of mastic chewing gum on reducing dental plaques and mouth infections.
6. Epitheorese Klinikes Farmakologias kai Farmakokinetikes, International Edition. 2001 16: 203-205.
The Antiphlogistic Action of the Chios Mastic Essential Oil: Treatment of Skin Irritations Resulting from Hair Epilation and peeling with lotions Containing Mastic Essential Oil.
E.E Protopapa, A. Heliou, A. Tsigonia, V. Kefala, A. Triantafyllou, J Tsaknis.
Technological Educational Institution, Athens, Technological Educational Institution, of Thessaloniki & Laboratory of Biologikal Chemistry, University of Athens Medical School.
The antiphlogistic effect of mastic essential oil was tested in skin subjected to epilation either by thermolysis or enzymes in different skin region and peeling. Creams containing mastic essential oil significantly reduced the time period necessary to relieve the irritatiom resulting from epilation or peeling, compared to skin regions treated with placebo cream devoid of the mastic oil.
Mastic gum is a viscous light-green liquid obtained from the bark of Pistacia lentiscus var. chia. which belongs to the Anacardiaceae family. The gum has been fractionated to investigate the antimicrobial activity of the whole gum and its fractions against various strains of Helicobacter pylori. The polymeric gum fraction was separated from the essential oil and the resin (trunk exudates without essential oil) to assess and compare the anti-H. pylori activity of the polymer fraction against lower molecular weight fractions, the gum itself and masticated gum. The polymer fraction was also oxidized and assessed for antimicrobial activity.
Keywords: Mastic Gum; Helicobacter pylori; Pistachia lentiscus.

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