Source: https://www.biology.pitt.edu/person/graham-hatfull
Timestamp: 2019-04-23 03:55:45+00:00

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Mycobacterium tuberculosis kills more people than any other single infectious agent. Since antibiotics are available and the BCG vaccine is in widespread use, why do two million people die each year from TB? The answer, in part, is that we really don't understand this curious bacterium or what parts of its genetic instructions make this such a deadly pathogen. At the heart of our strategies to understand mycobacterial genetics is the mycobacteriophages - viruses that infect the mycobacteria. These are easy to grow and manipulate and offer advantages over working with the slow-growing mycobacteria (such as M. tuberculosis) that can take up to a month to produce a colony on an agar plate. Phages are also rich sources of potential genetic and molecular tools that can be used to study - and to modify - their bacterial hosts.
Exploring bacteriophage genomics. In collaboration with Dr. Hendrix we have spearheaded an initiative to understand viral diversity and evolution. Our specific focus is on the genomic characterization of mycobactriophages, and a collection of about 250 complete genome sequences have been determined. Many of these phages were isolated and sequenced through three programs in which phage discovery and genomics is a platform for integrating our science and educational missions. These are the Pittsburgh Phage Hunters Integrating Research and Education (PHIRE) program, the Howard Hughes Medical Institute Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (HHMI SEA-PHAGES) program, and a Univiersity of KwaZulu-Natal and KwaZulu-Nalat Research in TB and HIV (UKZN/K-RITH) workshop. These studies have not only provided valuable insights into phage diveristy and evolution, but present a rich and easily-accessible reservoir of genetic and mechanistic novelty for further study. A database of mycobaectriophage genomic infomration is available at http://www.phagesdb.org.
Exploiting mycobacteriophages. We are dissecting the mycobacteriophages to understand the functional roles of the thousands of genes we have identified, and to deternine if and when they are expressed, and how this expression is regulated. We are exploiting this information to develop tools and approaches that not only generate new tools for genetic manipulation for tuberculosis, but also to gain advances in diagnosis, prevention and treatment of the disease.
Site-specific recombination. Many if not most of the mycobacteriophages we have sequenced integrate their DNA into the host chromosome (and can excise them too). We are studying the mechanism of integrase-mediated site-specific recombination with a primary current focus on the serine-integrases. We are partiualrly interested in understanding how recomibnational directionality is determine in phage integration systems.
Tools - Genetic and Clinical. Studying the mycobacteria and their phages has great potential for the development of novel tools for their genetics but also for a more direct clinical involvement. Two systems we have been involved in developing are multivalent recombinant BCG vaccines and Luciferase Reporter Phages, but there are numerous additional strategies awaiting further development!
Hughes, L. E., Shaffer, C. D., Ware, V. C., Aguayo, I., Aziz, R. M., Bhuiyan, S., Bindert, I. S., Calovich-Benne, C. K., Chapman, J., Donegan-Quick, R., Farooq, A., Garcia, C., Graham, L. H., Green, B. Y., Kenna, M. A., Kneeream, E. R., Laing, C. E., Mageeney, C. M., Meridew, S. N., Mikolon, A. R., Morgan, R. E., Nayek, S., Olugbade, I. D., Pike, K. C., Schlegel, L. E., Shishido, T. C., Suresh, T., Suri, N., Weston Hafer, K., Garlena, R. A., Russell, D. A., Cresawn, S. G., Pope, W. H., Jacobs-Sera, D., Hatfull, G. F. (2018). Eight Genome Sequences of Cluster BE1 Phages That Infect Streptomyces Species. Genome announcements 6.
Doyle, E. L., Fillman, C. L., Reyna, N. S., Tobiason, D. M., Westholm, D. E., Askins, J. L., Backus, B. P., Baker, A. C., Ballard, H. S., Bisesi, P. J., Bond, L., Byrnes, D., Carlstedt, H., Dodson, K. S., Fallert, M. J., Foster, K. J., Games, D. N., Grams, T. R., Guild, N. A., Hurd, A., Iwata, N., Kepler, C. R., Krenzke, L. R., Luekens, K., Lewis, J., McEntee, C., McGee, J. C., Nalley, N., Plymale, R. C., Prochaska, J., Rogers, R. G., Schipper, J. B., Snyder, K., Uhrich, K., Vermillion, C. D., Vickers, S. K., Wenta, M. D., Yates, T. Z., Young, C. F., Stoner, T. H., Pope, W. H., Jacobs-Sera, D., Garlena, R. A., Russell, D. A., Cresawn, S. G., Hatfull, G.F. (2018). Genome Sequences of Four Cluster P Mycobacteriophages. Genome announcements 6.
Anders, K.R., Barekzi, N., Best, A.A., Frederick, G.D., Mavrodi, D.V., Vazquez, E., Sea, P., Amoh, N.Y.A., Baliraine, F.N., Buchser, W.J., Cast, T.P., Chamberlain, C.E., Chung, H.M., D'Angelo, W.A., Farris, C.T., Fernandez-Martinez, M., Fischman, H.D., Forsyth, M.H., Fortier, A.G., Gallo, K.F., Held, G.J., Lomas, M.A., Maldonado-Vazquez, N.Y., Moonsammy, C.H., Namboote, P., Paudel, S., Polley, S.M., Reyes, G.M., Rubin, M.R., Saha, M.S., Stukey, J., Tobias, T.D., Garlena, R.A., Stoner, T.H., Cresawn, S.G., Jacobs-Sera, D., Pope, W.H., Russell, D.A., Hatfull, G.F., 2017. Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn. Genome announcements 5.
Bonilla, J. A., Isern, S., Findley, A. M., Klyczek, K. K., Michael, S. F., Saha, M. S., Buchser, W.J., Forsyth, M. H., Paudel, S., Gissendanner, C. R., Wiedemeier, A. M. D., Alonzo, F. L., University of Wisconsin-River Falls, SEA-PHAGES, Florida Gulf Coast University, SEA-PHAGES, University of Louisiana-Monroe, SEA-PHAGES, College of William and Mary SEA-PHAGES, Garlena, R. A., Russell, D. A., Pope, W. H., Cresawn, S. G., Jacobs-Sera, D., Hatfull, G. F. (2017). Genome Sequences of 19 Rhodococcus erythropolis Cluster CA Phages. Genome announcements 5.
Hanauer, D. I., Graham, M. J., SEA-PHAGES, Betancur, L., Bobrownicki, A., Cresawn, S. G., Garlena, R. A., Jacobs-Sera, D., Kaufman, N., Pope, W. H., Russell, D. A., Sivanathan, V., Jacobs Jr., W.R., Asai, D. J., Hatfull, G. F. (2017). An Inclusive Research-Education Community (iREC): A model for student engagement in science. Proc. Natl.Acad. Sci. USA. 114, 13531-13536. doi:10.1073/pnas.1718188115. PMID: 29208718.
Mehla, J., Dedrick, R. M., Caufield, J. H., Wagemans, J., Sakhawalkar, N., Johnson, A., Hatfull, G. F. and Uetz, P. 2017. Virus-host protein-protein interactions of mycobacteriophage Giles. Sci. Rep. 7, 16514.
Dedrick, R.M., Mavrich, T.N., Ng, W.L. and Hatfull, G.F. (2017) Expression and evolutionary patterns of mycobacteriophage D29 and its temperate close relatives. BMC Microbiol 17: 225.
Mehla, J., R.M. Dedrick, J.H. Caufield, J. Wagemans, N. Sakhawalkar, A. Johnson, G.F. Hatfull & P. Uetz, (2017) Virus-host protein-protein interactions of mycobacteriophage Giles. Sci Rep 7: 16514.
Herren, C.D., A. Peister, T.S. Breton, M.S. Hill, M.S. Anderson, A.W. Chang, S.B. Klein, M.M. Thornton, S.J. Vars, K.E. Wagner, P.L. Wiebe, T.G. Williams, C.P. Yanez, J.M. Ackles, D. Artis, R.J. Brazier, R.J. Bryant, K.O. Callwood, I.H. Carter, C.L. DeBose, C.D. Edwards, I.C. Ezemba, R.R. Joaquin, Z.M. Meghoo-Peddie, Z.G. Meghoo-Peddie, R.W. Moore, C.E. Smith, A.J. Turner, R.L. Vorters, J.J. Wider, L.L. Krout, M.S. Comis, M.J. Davick, E.E. Michaud, B.E. Shevenell, S.E. Stanley, C.I. Frank, J.R. Montgomery, L.S. Blumer, J.A. Doty, M. Smith-Caldas, W.H. Pope, S.G. Cresawn, D.A. Russell, R.A. Garlena, D. Jacobs-Sera & G.F. Hatfull, (2017) Genome Sequences of Four Subcluster L2 Mycobacterium Phages, Finemlucis, Miley16, Wilder, and Zakai. Genome announcements 5.
Edgington, N.P., S.M. Voshell, V.C. Ware, F.F.
Edgington, N.P., S.M. Voshell, V.C. Ware, F.F. Akoto, A.A. Alhout, G.J. Atwal, J.B. Balyozian, Z.A. Cadieux, B.M. Chop, S.G. Cresawn, N. Cudkevich, D.Z. Faltine-Gonzalez, R.A. Garlena, B.J. Gilmer, L.H. Graham, M.S. Grapel, M.M. Haleem, D. Jacobs-Sera, M.A. Kenna, M.A. Khan, T.N. Klein, J.B. Korenberg, B.P. Lichak, C.M. Mageeney, L.N. McKinley, K.R. Mendello, C.M. Myers, A.T. Nguyen, B.A. Pasqualucci, W.H. Pope, L.M. Pyfer, W.A. Ramirez, J.R. Reisner, D.A. Russell, P.A. Sapao, V.C. Saux, I. Singh, T.H. Stoner, R.H. Swope, M.J. Thoonkuzhy, M.L. Walters, L.A. Vargas, C.A. Veliz, K.D. Zhang, C.M. Zuilkoski & G.F. Hatfull, (2017) Genome Sequences of Chancellor, Mitti, and Wintermute, Three Subcluster K4 Phages Isolated Using Mycobacterium smegmatis mc2155. Genome announcements 5.
Chung, H.M., T. D'Elia, J.F. Ross, S.M. Alvarado, M.C. Brantley, L.P. Bricker, C.R. Butler, C. Crist, J.M. Dane, B.W. Farran, S. Hobbs, M. Lapak, C. Lovell, N. Ludergnani, A. McMullen, S.A. Mirza, N. Thrift, D.P. Vaughan, G. Worley, A. Ejikemeuwa, M. Zaw, C.F. Albritton, S.C. Bertrand, S.S. Chaudhry, V.A. Cheema, C. Do, M.L. Do, H.M. Duong, D.H. El-Desoky, K.M. Green, R.N. Lee, L.A. Thornton, J.M. Vu, M.N. Zahra, S.G. Cresawn, T.H. Stoner, R.A. Garlena, D. Jacobs-Sera, W.H. Pope, D.A. Russell & G.F. Hatfull, (2017) Complete Genome Sequences of Mycobacteriophages Clautastrophe, Kingsolomon, Krypton555, and Nicholas. Genome announcements 5.
Adair, T.L., E. Stowe, M.C. Pizzorno, G. Krukonis, M. Harrison, S.-P. Baylor University, S.-P. Bucknell University, S.-P. Cabrini University, S.G. Cresawn, R.A. Garlena, D.A. Russell, W.H. Pope, D. Jacobs-Sera & G.F. Hatfull, (2017b) Genome Sequences of Three Cluster AU Arthrobacter Phages, Caterpillar, Nightmare, and Teacup. Genome announcements 5.
Adair, T.L., J.A. Bonilla, K.K. Klyczek, J.Y. Lee-Soety, G. Rosas-Acosta, M. Harrison, S.-P. Baylor University, S.-P. University of Wisconsin-River Falls, S. Saint Joseph's University Phage, S.-P. University of Texas at El Paso, S.-P. Cabrini University, C.A. Bowman, S.G. Cresawn, R.A. Garlena, D.A. Russell, W.H. Pope, D. Jacobs-Sera & G.F. Hatfull, (2017a) Complete Genome Sequences of Arthrobacter Phages Beans, Franzy, Jordan, Piccoletto, Shade, and Timinator. Genome announcements 5.
Butela, K.A., S.M.R. Gurney, H.L. Hendrickson, J.M. LeBlanc-Straceski, A.M. Zimmerman, S.B. Conant, N.E. Freed, O.K. Silander, J.J. Thomson, C.A. Berkes, C. Bertolez, C.G. Davies, A. Elinsky, A.J. Hanlon, J. Nersesyan, P. Patel, J. Sherwood, T. Tieu Ngo, K.A. Wisniewski, K. Yacoo, P.M. Arendse, N.W. Bowlen, J. Cunmulaj, J.L. Downs, C.A. Ferrenberg, A.E. Gassman, C.E.R. Gilligan, E. Gorkiewicz, C. Harness, A. Huffman, C. Jones, A. Julien, A.E. Kupic, S.F. Latu, T.J. Manning, D. Maxwell, S.E.A.P.A. Merrimack College, C.E. Meyer, M. Reardon, M. Slaughter, R. Swasey, R.I. Tennent, V. Torres, T. Waller, R.M. Worcester, B.L. Yost, S.G. Cresawn, R.A. Garlena, D. Jacobs-Sera, W.H. Pope, D.A. Russell, G.F. Hatfull & J.D. Kagey, (2017) Complete Genome Sequences of Cluster A Mycobacteriophages BobSwaget, Fred313, KADY, Lokk, MyraDee, Stagni, and StepMih. Genome announcements 5.
Pope, W.H., T.N. Mavrich, R.A. Garlena, C.A. Guerrero-Bustamante, D. Jacobs-Sera, M.T. Montgomery, D.A. Russell, M.H. Warner, G. Science Education Alliance-Phage Hunters Advancing, S. Evolutionary & G.F. Hatfull, (2017) Bacteriophages of Gordonia spp. Display a Spectrum of Diversity and Genetic Relationships. mBio 8.
Mavrich, T.N., and Hatfull, G. F. &nbsp;(2017).
Klyczek, K. K., J. A. Bonilla, D. Jacobs-Sera, T. L. Adair, P. Afram, K. G. Allen, M. L. Archambault, R. M. Aziz, F. G. Bagnasco, S. L. Ball, N. A. Barrett, R. C. Benjamin, C. J. Blasi, K. Borst, M. A. Braun, H. Broomell, C. B. Brown, Z. S. Brynell, A. B. Bue, S. O. Burke, W. Casazza, J. A. Cautela, K. Chen, N. S. Chimalakonda, D. Chudoff, J. A. Connor, T. S. Cross, K. N. Curtis, J. A. Dahlke, B. M. Deaton, S. J. Degroote, D. M. DeNigris, K. C. DeRuff, M. Dolan, D. Dunbar, M. S. Egan, D. R. Evans, A. K. Fahnestock, A. Farooq, G. Finn, C. R. Fratus, B. L. Gaffney, R. A. Garlena, K. E. Garrigan, B. C. Gibbon, M. A. Goedde, C. A. Guerrero Bustamante, M. Harrison, M. C. Hartwell, E. L. Heckman, J. Huang, L. E. Hughes, K. M. Hyduchak, A. E. Jacob, M. Kaku, A. W. Karstens, M. A. Kenna, S. Khetarpal, R. A. King, A. L. Kobokovich, H. Kolev, S. A. Konde, E. Kriese, M. E. Lamey, C. N. Lantz, J. S. Lapin, T. O. Lawson, I. Y. Lee, S. M. Lee, J. Y. Lee-Soety, E. M. Lehmann, S. C. London, A. J. Lopez, K. C. Lynch, C. M. Mageeney, T. Martynyuk, K. J. Mathew, T. N. Mavrich, C. M. McDaniel, H. McDonald, C. J. McManus, J. E. Medrano, F. E. Mele, J. E. Menninger, S. N. Miller, J. E. Minick, C. T. Nabua, C. K. Napoli, M. Nkangabwa, E. A. Oates, C. T. Ott, S. K. Pellerino, W. J. Pinamont, R. T. Pirnie, M. C. Pizzorno, E. J. Plautz, W. H. Pope, K. M. Pruett, G. Rickstrew, P. A. Rimple, C. A. Rinehart, K. M. Robinson, V. A. Rose, D. A. Russell, A. M. Schick, J. Schlossman, V. M. Schneider, C. A. Sells, J. W. Sieker, M. P. Silva, M. M. Silvi, S. E. Simon, A. K. Staples, I. L. Steed, E. L. Stowe, N. A. Stueven, P. T. Swartz, E. A. Sweet, A. T. Sweetman, C. Tender, K. Terry, C. Thomas, D. S. Thomas, A. R. Thompson, L. Vanderveen, R. Varma, H. L. Vaught, Q. D. Vo, Z. T. Vonberg, V. C. Ware, Y. M. Warrad, K. E. Wathen, J. L. Weinstein, J. F. Wyper, J. R. Yankauskas, C. Zhang & G. F. Hatfull, (2017) Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages. PLoS One 12: e0180517.
Jacobs-Sera, D., O. Catinas, M. Fernandez-Martinez, A. Garcia, R.A. Garlena, C.A. Guerrero Bustamante, M.H. Larsen, R.H. Medellin, M.Y. Melendez-Ortiz, C.M. Melendez-Rivera, A.K. Mercado-Andino, A.J. Mercado-Delgado, C.P. Ortiz-Ortiz, A.M. Quesada-Gordillo, J.M. Ramos, M.R. Rubin, D.A. Russell, R.A. Sadana, S. Saha, E. Vazquez, D. Villarreal & G.F. Hatfull, (2017) Genome Sequences of Mycobacteriophages Kerberos, Pomar16, and StarStuff. Genome announcements 5.
Russell, D. A. and Hatfull, G. F. &nbsp;(2017).
Russell, D. A. and Hatfull, G. F. (2017). PhagesDB: The actinobacteriophage Database. Bioinformatics 33, 784-786. doi.org/10.1093/bioinformatics/btw711.
Hatfull, G. F. &amp; Vehring, R. &nbsp;(2016).
Hatfull, G. F. & Vehring, R. (2016). Respirable Bacteriophage Aerosols for the Prevention and Treatment of Tuberculosis in Drug Delivery Systems for Tuberculosis Prevention and Treatment. Ed. Hickey, A. J. Wiley. pp277-292.
Keenholtz, R. A., Grindley, N. D., Hatfull, G.
Hanauer, D. I., Graham, M. J., and Hatfull, G.
Bollivar, D. W., Bernardoni, B., Bockman, M. R.
Urdaniz, E., Rondon, L., Marti, M., Hatfull, G.
Pope, W. H. & Hatfull, G. F. (2016). Adding pieces to the puzzle: New insights into bacteriophage diversity from integrated research-education programs. Bacteriophage 5(4):e1084073.
Hatfull, G. F. (2016). In the Trenches of Microbial Warfare: Identification of Genes and Pathways Contributing to Bacteriophage Infection by Quantitative Selection Analysis. J. Mol. Biol. 428, 413-415.
Petrova, Z. O., Broussard, G. W. and Hatfull, G. F. (2015). Mycobacteriophage-repressor mediated immunity as selectable genetic markers: Adephagia and BPs repressor-selection. Microbiology In press.
Mehla, J., R.M. Dedrick, J.H. Caufield, R. Siefring, M. Mair, A. Johnson, G.F. Hatfull & P. Uetz, (2015) The protein interactome of mycobacteriophage Giles predicts functions for unknown proteins. J Bacteriol. 197, 2508-2516.
Pope, W.H., E.A. Bina, I.S. Brahme, A.B. Hill, P.H. Himmelstein, S.M. Hunsicker, A.R. Ish, T.S. Le, M.M. Martin, C.N. Moscinski, S.A. Shetty, T. Swierzewski, V.B. Iyengar, H. Kim, C.E. Schafer, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequence of Mycobacteriophage Momo. Genome announcements 3.
Pope, W.H., E. Jacobetz, C.A. Johnson, B.L. Kihle, M.A. Sobeski, M.B. Werner, N.L. Adkins, Z.J. Kramer, M.T. Montgomery, S.R. Grubb, M.H. Warner, C.A. Bowman, D.A. Russell & G.F. Hatfull, (2015) Genome Sequence of Mycobacteriophage Phayonce. Genome announcements 3.
Hatfull, G.F. (2015). Dark matter of the biosphere: The amazing world of bacteriophage diversity. J. Virol. 89, 8107-8110.
Hatfull, G.F. (2015). Innovations in Undergraduate Science Education: Going Viral. J Virol. 89, 8111-8113.
Pope, W.H., Bowman, C.A., Russell, D.A., Jacobs-Sera, D., Asai, D.J., Cresawn, S.G., Jacobs, W.R., Hendrix, R.W., Lawrence, J.G., Hatfull, G.F., et al. (2015). Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity. Elife 4.
Ojha, A. K., Jacobs, W R. Jr., and Hatfull, G.
Ojha, A. K., Jacobs, W R. Jr., and Hatfull, G. F. (2015). Genetic Dissection of Mycobacterial Biofilms in Mycobacteria Protocols. Methods in Molecular Biology 1285, 215-226.
Casjens S. R., Jacobs-Sera, D., Hatfull, G. F., Hendrix, R. W. (2015) Genome sequence of Salmonella enterica phage Det7. Genome Announc. May 7;3(3). pii: e00279-15. doi: 10.1128/genomeA.00279-15.
Hendrix, R. W., Ko, C. C., Jacobs-Sera, D., Hatfull, G. F., Erhardt, M., Hughes, K. T,, Casjensm S. R. (2015) Genome Sequence of Salmonella Phage χ. Genome Announc. 3. pii: e01229-14.
Pope, W. H., Jacobs-Sera, D., Russell, D. A., Rubin, D. H. F., Kajee, A., Msibi, Z. N. P., Larsen, M. H., Jacobs, W. R. Jr., Lawrence, J. G., Hendrix, R. W., & Hatfull, G. F. (2014). Genomics and proteomics of Mycobacteriophage Patience: An accidental tourist in the Mycobacterium neighborhood mBio pii: e02145-14. doi: 10.1128/mBio.02145-14.
Hatfull, G. F. (2014) Molecular Genetics of Mycobacteriophages. Microbiol Spectr. 2, 1-36.
Hatfull, G. F. and Racaniello, V. (2014). PHIRE and TWiV: Experiences in bringing virology to new audiences. Ann. Rev. Virology. 1, 37-53.
Hatfull G. F. & Jacobs W.R Jr.(eds) Molecular Genetics of Mycobacteria. (2014). Second edition. ASM Press. Washington, DC.
Oldfield, L. M. and Hatfull, G. F. (2014). Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J. Bacteriol. 196, 3589-3597.
Hatfull, G. F. (2014). Molecular Genetics of Mycobacteriophages in Molecular Genetics of Mycobacteria, Second Edition. Eds. Hatfull, G. F. & Jacobs, W. R. Jr. ASM press, Washington, DC.
Russell, D. A., Bowman, C. A., and Hatfull, G.
Dieterle, M. E., Jacobs-Sera, D., Russell, D., Hatfull, G., Piuri, M. (2014) Complete Genome Sequences of Lactobacillus Phages J-1 and PL-1. Genome Announc 2.
Singh, S., Plaks, S. G., Homa, N. J., Amrich, C. G., Heroux, A., Hatfull, G. F., & VanDemark, A. P. (2014). The structure of Xis reveals the basis for filament fomration and insight into DNA bending within a mycobacteriophage Intasome. J. Mol. Biol., 426, 412-422.
Singh. S., Rockenbach, K., Dedrick, R. M., VanDemark, A. P., & Hatfull, G. F. (2014) Cross-talk between Diverse Serine Integrases. J Mol Biol 426, 318-331.
Smith, M. C., Hendrix, R. W., Dedrick, R., Mitchell, K., Ko, CC., Russell, D., Bell, E., Gregory, M., Bibb, M. J., Pethick, F., Jacobs-Sera, D., Herron, P., Buttner, M. J., and Hatull, G. F. (2013). Evolutionary relationships within actinophages and a putative adaptation for growth in Streptomyces spp. J. Bacteriol. 195, 4924-4935.
Hatfull, G. F. Science Education Alliance Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) program, KwaZulu-Natal Research Institute for Tuberculosis and HIV (K-RITH) Mycobacterial Genetics Course, University of California Los Angeles Research Immersion Laboratory in Virology, Phage Hunters Integrating Research and Education (PHIRE) program. (2013). The complete genome sequences of 63 mycobacteriophages. GenomeA 1, doi:pii: e00847-13. 10.1128/genomeA.00847-13.
Piuri, M., Rondon, L., Urdaniz, E., and Hatfull, G. F. (2013). Generation of affinity-tagged fluoromycobacteriophages by mixed assembly of phage capsids. Appl Environ Microbiol. 79, 5608-5615.
Pope, W. H., Jacobs-Sera, D., Best, A. A., Broussard, G. W., Connerly, P. L., Dedrick, R. M., Kremer, T. A., Offner, S., Ogiefo, A. H., Pizzorno, M. C., Rockenbach, K., Russell, D. A., Stowe, E. L., Stukey, J., Thibault, S. A., Conway, J. F., Hendrix, R. W., Hatfull, G. F. (2013) Cluster J mycobacteriophages: intron splicing in capsid and tail genes. PLoS One 8:e69273.
Broussard, G. W. and Hatfull, G. F. (2013) Evolution of genetic switch complexity. Bacteriophage 3:e24186.
Sambandan, D., D.N. Dao, B.C. Weinrick, C. Vilcheze, S.S. Gurcha, A. Ojha, L. Kremer, G.S. Besra, G.F. Hatfull & W.R. Jacobs, Jr., (2013) Keto-mycolic acid-dependent pellicle formation confers tolerance to drug-sensitive Mycobacterium tuberculosis. MBio 4: e00222-00213.
Hatfull, G. F. (Ed). Recent Progress in Bacteriophage Research (2013). Multidisciplinary Digital Publishing Institute, Basel.
Silva, J.L., M. Piuri, G. Broussard, L. Marinelli, G.M. Bastos, R.D. Hirata, G.F. Hatfull & M.H. Hirata, (2013) Application of BRED technology to construct recombinant D29 reporter phage expressing EGFP. FEMS Microbiol Lett. 344, 166-172.
Dedrick, R. M., Marinelli, L. J., Newton, G. L.
Dedrick, R. M., Marinelli, L. J., Newton, G. L., Pogliano, K., Pogliano, J ., Hatfull, G. F. (2013). FUnctional requirements for bacetriophage growth: gene essentiality and expression in mycobacteriophage Giles. Mol. Microbiology EPub head of print.
Broussard, G.W., L.M. Oldfield, V.M. Villanueva, B.L. Lunt, E.E. Shine & G.F. Hatfull, (2013) Integration-Dependent Bacteriophage Immunity Provides Insights into the Evolution of Genetic Switches. Mol Cell. 49, 237-248.
Marinelli, L. J. , Hatfull, G. F., Piuri, M. (2012) Recombineering: A powerful tool for modification of bacteriophage genomes. Bacteriophage 2, 5-14.
Hatfull (2012). The Secret Lives of Mycobacteriophages. Advances in Virus Research. 82,179-288.
Hatfull, G. F., et al (2012) Complete genome sequences of 138 mycobacteriophages.&nbsp; J. Virol. 86: 2382-2384.
Hatfull, G. F., et al (2012) Complete genome sequences of 138 mycobacteriophages. J. Virol. 86: 2382-2384.
Kulka, K., Hatfull, G., and Ojha, A.K. (2012).
Savinov, A., Pan, J., Ghosh, P. and Hatfull, G. F. (2012) The Bxb1 gp47 recombination directionality factor is required not only for prophage excision but also for phage DNA replication.&nbsp; Gene 495: 42-48.
Savinov, A., Pan, J., Ghosh, P. and Hatfull, G. F. (2012) The Bxb1 gp47 recombination directionality factor is required not only for prophage excision but also for phage DNA replication. Gene 495: 42-48.
Cresawn, S.G., Bogel, M., Day, N., Jacobs-Sera, D., Hendrix, R.W. and Hatfull, G.F. (2011) Phamerator: a bioinformatic tool for comparative bacteriophage genomics. BMC Bioinformatics 12:395.
Anderson, W.A., Amasino, R.M., Ares, M., Jr., Banerjee, U., Bartel, B., Corces, V.G., Drennan, C.
Bai, H., Sun, M., Ghosh, P., Hatfull, G.F., Grindley, N.D. and Marko, J.F. (2011) Single-molecule analysis reveals the molecular bearing mechanism of DNA strand exchange by a serine recombinase. Proc Natl Acad Sci U S A 108:7419-24.
Huang, J., Ghosh, P., Hatfull, G.F. and Hong, Y. (2011) Successive and targeted DNA integrations in the Drosophila genome by Bxb1 and phiC31 integrases. Genetics 189:391-5.
Marine, R., Polson, S.W., Ravel, J., Hatfull, G., Russell, D., Sullivan, M., Syed, F., Dumas, M.
Marine, R., Polson, S.W., Ravel, J., Hatfull, G., Russell, D., Sullivan, M., Syed, F., Dumas, M. and Wommack, K.E. (2011) Evaluation of a transposase protocol for rapid generation of shotgun high-throughput sequencing libraries from nanogram quantities of DNA. Appl Environ Microbiol 77: 8071-9.
Jain, P., Thaler, D.S., Maiga, M., Timmins, G.S., Bishai, W.R., Hatfull, G.F., Larsen, M.H. and Jacobs, W.R. (2011) Reporter phage and breath tests: emerging phenotypic assays for diagnosing active tuberculosis, antibiotic resistance, and treatment efficacy. J Infect Dis 204: 1142-1150.
Anderson, W.A., Banerjee, U., Drennan, C.L., Elgin, S.C., Epstein, I.R., Handelsman, J., Hatfull, G.F., Losick, R., ODowd, D.K., Olivera, B.M., Strobel, S.A., Walker, G.C. and Warner, I.M. (2011) Science education. Changing the culture of science education at research universities. Science 331:152-153.
Stewart, C.R., S.R. Casjens, S.G. Cresawn, J.M. Houtz, A.L. Smith, M.E. Ford, C.L. Peebles, G.F.
Dr. Hatfull received his Ph.D. in 1981 with Willie Donachie at the University of Edinburgh, Scotland, performed postdoctoral studies with Nigel Grindley at Yale University and with Fred Sanger at the MRC, and joined the Department in 1988.

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