Source: http://2018.igem.org/Team:Marburg/Team
Timestamp: 2019-04-26 09:57:20+00:00

Document:
We as iGEM Team Marburg 2018 are very proud to present this years team. The team consists of 15 team members from various different fields ranging from traditional biology, Molecular and Cellular Biology, chemistry, physics to computer science and this made it possible to work in a very interdisciplinary environment. It was only possible to do this project thanks to our two team leaders, our instructor, and our two PIs. Last but not least we want to thank our amazing board of 8 advisors because of their continuous support the project became what it is today.
Favorite Bacterium Vibrio natriegens - are there other bacteria out there?
The moment I loved working with V. natriegens The moment when I wanted to prepare competent cells and took them out of the shaker with OD 0.4. Shaking by hand for five minutes and I had them ready for harvesting with OD 0.5.
Favorite Bacterium: Myxococcus xanthus - because it forms sun shaped spots on agar plates.
The moment I loved working with V. natriegens: When I forgot to incubate plates at 37°C and could nevertheless perform colony PCR after 3 hours.
The moment I loved working with V. natriegens: After we created the first growth curve and showed how f***ing incredible fast it grows.
Favorite Bacterium: Streptomyces venezuelae - because i like the hyphal growth and chloramphenicol!
The moment I loved working with V. natriegens: When i could leave early because I could quickly pick colonies from a plate I had made after lunch.
The moment I hated working with V. natriegens: When we still worked with E. coli, mistakenly plating V. natriegens on LB instead of LBv2 plates.
The moment I hated working with V. natriegens: I had to inoculate a liquid culture four times to get cells in their exponential phase because they grew just too fast!
Favorite Bacterium: Vibrio harveyi - they can make big regions of sea get glowing if they work together. They are a model organism for Quorum sensing what shows again that Bacteria are not as simple as common people think.
The moment I loved working with V. natriegens: The time before Wiki freeze because when you need a new part and a cloning result best at the end of the day, you can actually get it.
The moment I hated working with V. natriegens: Definitely building a LVL2 Part in 3 days! And when your CRISPR colonies are taking much longer than you are used to for colonies appearing.
The moment I loved working with V. natriegens: The moment you realize that you have forgotten to start an overnight culture and still be able to do a plasmid preparation that day.
The moment I hated working with V. natriegens: The moment when your culture is overgrown for the hundredth time.
The moment I hated working with V. natriegens: When I had to pronounce it correctly for the first time. Why isn’t it called Vibrio baam?
The moment I loved working with V. natriegens: not having to work in a lab.
The moment I hated working with V. natriegens: not having to work in a lab.
Favorite Bacterium: Cyanobacterium - For its versatility in, and impact on nature and science. its important connection to biological evolution and its amazing potential for semi-artificial photosynthesis and other biotechnological applications.
The moment I loved working with V. natriegens: The immediate success I achieved with the cotransformation and other electroporation of our constructs. It was my first ever transformation. And it worked perfectly in Vibrio natriegens.
Favorite Bacterium: Vibrio natriegens - the future!
The moment I loved working with V. natriegens: Being able to share this incredibly fast organism with the other iGEM teams through the Vibrigens collaborations.
The moment I hated working with V. natriegens: (Some of) the other iGEM teams finding it too fast.
Favorite Bacterium: Pseudomonas syringae - because it can make it snow!
The moment I loved working with V. natriegens: When going home early because I could pick after just few hours.
The moment I hated working with V. natriegens: When I smelled old plates with V. natriegens on it.
The moment I loved working with V. natriegens: When i saw how dense it could grow!
The moment I hated working with V. natriegens: When my mini prep did not work because my culture grew to dens.

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