Source: http://www.proceedings.blucher.com.br/article-details/extrao-de-lpidios-de-microalga-avaliao-da-qualidade-utilizando-espectroscopia-de-infravermelho-ft-ir-11068
Timestamp: 2019-04-18 22:42:05+00:00

Document:
Pouco se relata sobre a qualidade de lípideos extraidos de microalgas para obtenção de biocombustíveis. Assim, o objetivo deste trabalho foi extrair lipídios a partir da biomassa seca da microalga Spirulina sp. LEB 18 utilizando os seguintes métodos: (A) Bligh e Dyer, (B) Folch, (C) Soxhlet utilizando álcool isopropílico e vácuo, (D) Soxhlet utilizando álcool isopropílico sem vácuo, (E) Soxhlet utilizando hexano e vácuo e (F) Soxhlet utilizando hexano sem vácuo. O teor de lipídios foi obtido gravimetricamente e sua qualidade verificada através de análise de infravermelho com transformada de Fourier (FTIR-ATR). Os resultados indicaram que o método D foi o mais eficiente para extração, apresentando teor de lipídios de 10,3%. Os espectros de infravermelho mostraram que bandas características de triglicerídeos foram observadas nos lipídios independente do método de extração. A banda em 3350 cm-1, correspondente ao estiramento dos grupos ligados ao hidrogênio O-H e N-H, bem como a banda em 1650 cm-1 proveniente de carbonos constituintes de anéis aromáticos, não foram identificadas para as amostras extraídas nos métodos E e F. Esses resultados mostraram que o produto extraído pelos métodos E e F foi mais seletivo em relação a extração de triglicerídeos.
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