Source: https://svi.nl/HuygensReferences
Timestamp: 2019-04-21 03:08:43+00:00

Document:
"99 out of the top 100 universities (World University Rakings 2017) use Huygens!
Independant scientists worldwide chose Huygens as the best deconvolution technique. Deconvolution puts you at the forefront of biological imaging and boosts your research.''.
Zandt, B. J., Liu, J. H., Veruki, M. L., & Hartveit, E. (2017). AII amacrine cells: quantitative reconstruction and morphometric analysis of electrophysiologically identified cells in live rat retinal slices imaged with multi-photon excitation microscopy. Brain Structure and Function, 222(1), 151-182.
"Imaging in 3 dimensions in STED is also possible, and will also give an improvement over conventional confocal imaging. This is particularly true if it is done in combination with deconvolution (ed: with Huygens)”.
Mace EM, Orange JS. (2014). Visualization of the immunological synapse by dual color time-gated stimulated emission depletion (STED) nanoscopy.. J Vis Exp. 2014 Mar 24;(85): 51100.
Day, K. J., La Rivière, P. J., Chandler, T., Bindokas, V. P., Ferrier, N. J., & Glick, B. S. (2017). Improved deconvolution of very weak confocal signals.
Kochan, J., Wawro, M., & Kasza, A. (2016). IF-combined smRNA FISH reveals interaction of MCPIP1 protein with IER3 mRNA. Biology open, bio-018010.
Many top scientific papers refer to the use of the SVI's software. Below is just a selection of these papers. If you have used a SVI product for a publication it is common practise to describe the use correctly (see How to cite us). If you have already published an article or book (chapter) with Huygens, we like to congratulate you and ask you to be included in the list below by filling out this form or by sending the reference to support@svi.nl.
What follows are mostly references that Huygens users asked to be included here, but many others are available for example in Google Scholar.
Gupta, S. K., Li, Y., & Guo, M. (2019). Anisotropic mechanics and dynamics of a living mammalian cytoplasm. Soft matter, 15(2), 190-199.
Foidl, B. M., & Humpel, C. (2019). Chronic treatment with five vascular risk factors causes cerebral amyloid angiopathy but no Alzheimer pathology in C57BL6 mice. Brain, behavior, and immunity.
Baharlou, H., Canete, N. P., Bertram, K. M., Sandgren, K. J., Cunningham, A. L., Harman, A. N., & Patrick, E. (2019). Digital removal of autofluorescence from microscopy images. bioRxiv, 566315.
Bartolomé-Martín, D., Ibáñez, I., Piniella, D., Martínez-Blanco, E., Pelaz, S. G., & Zafra, F. (2019). Identification of potassium channel proteins Kv7. 2/7.3 as common partners of the dopamine and glutamate transporters DAT and GLT-1. Neuropharmacology.
Bentivoglio, M., Cotrufo, T., Ferrari, S., Tesoriero, C., Mariotto, S., Bertini, G., ... & Mazzarello, P. (2019). The original histological slides of Camillo Golgi and his discoveries on neuronal structure. Frontiers in neuroanatomy, 13.
Sauer, F., Oswald, L., de Schellenberger, A. A., Tzschätzsch, H., Schrank, F., Fischer, T., ... & Käs, J. A. (2019). Collagen networks determine viscoelastic properties of connective tissues yet do not hinder diffusion of the aqueous solvent. Soft Matter.
Kelley, J. B., & Paschal, B. M. (2019). Fluorescence-based quantification of nucleocytoplasmic transport. Methods, 157, 106-114.
Pandey, G., Westhoff, J. H., Schaefer, F., & Gehrig, J. (2019). A Smart Imaging Workflow for Organ-Specific Screening in a Cystic Kidney Zebrafish Disease Model. International journal of molecular sciences, 20(6), 1290.
Bürgers, J., Pavlova, I., Rodriguez-Gatica, J. E., Henneberger, C., Oeller, M., Ruland, J. A., ... & Schwarz, M. K. (2019). Light-sheet fluorescence expansion microscopy: fast mapping of neural circuits at super resolution. Neurophotonics, 6(1), 015005.
Velazquez, F. N., Miretti, M., Baumgartner, M. T., Caputto, B. L., Tempesti, T. C., & Prucca, C. G. (2019). Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study. Scientific reports, 9(1), 3010.
Souriant, S., Balboa, L., Dupont, M., Pingris, K., Kviatcovsky, D., Cougoule, C., ... & Raynaud-Messina, B. (2019). Tuberculosis Exacerbates HIV-1 Infection through IL-10/STAT3-Dependent Tunneling Nanotube Formation in Macrophages. Cell Reports, 26(13), 3586-3599.
Fekete, A., Nakamura, Y., Yang, Y. M., Herlitze, S., Mark, M. D., DiGregorio, D. A., & Wang, L. Y. (2019). Underpinning heterogeneity in synaptic transmission by presynaptic ensembles of distinct morphological modules. Nature communications, 10(1), 826.
Hoette, K., Koch, M., Hof, L., Tuppi, M., Moreth, T., Stelzer, E. H., & Pampaloni, F. (2019). Ultra-thin fluorocarbon foils optimize multiscale imaging of three-dimensional native and optically cleared specimens. bioRxiv, 533844.
Bucevičius, J., Keller-Findeisen, J., Gilat, T., Hell, S. W., & Lukinavičius, G. (2019). Rhodamine–Hoechst positional isomers for highly efficient staining of heterochromatin. Chemical Science.
Colin-York, H., Li, D., Korobchevskaya, K., Chang, V. T., Betzig, E., Eggeling, C., & Fritzsche, M. (2019). Cytoskeletal actin patterns shape mast cell activation. Communications Biology, 2(1), 93.
Bhukel, A., Beuschel, C. B., Maglione, M., Lehmann, M., Juhász, G., Madeo, F., & Sigrist, S. J. (2019). Autophagy within the mushroom body protects from synapse aging in a non-cell autonomous manner. Nature communications, 10(1), 1318.
Gao, G., Zhu, C., Liu, E., & Nabi, I. R. (2019). Reticulon and CLIMP-63 control nanodomain organization of peripheral ER tubules. bioRxiv, 550715.
Gulvady, A. C., Forsythe, I. J., & Turner, C. E. (2019). Hic-5 regulates Src-induced invadopodia rosette formation and organization. Molecular biology of the cell, mbc-E18.
Hartveit E., Zandt B-J., Madsen E., Castilho A., Mørkve S.H., Veruki M.L., (2018). AMPA receptors at ribbon synapses in the mammalian retina: kinetic models and molecular identity.. Brain Struct Funct. 2018 Mar;223(2):769-804.
Huygens was used for deconvolution, and the Huygens Object Analyzer was used to determine "the spatial relationships between fluorescent structures corresponding to (dye-filled) AII amacrine cell and immunolabeled punctae."
Spiess M., Hernandez‐Varas P., Oddone A., Olofsson H., Blom H., Waithe D., Lock J.G., Lakadamyali M., Strömblad S., (2018). Active and inactive β1 integrins segregate into distinct nanoclusters in focal adhesions. Journal of Cell Biol. 217(6):1929-1940.
Resnik, N., Prezelj, T., De Luca, G., Manders, E., Polishchuk, R., Veranič, P., Kreft, M.E., (2018). Helical organization of microtubules occurs in a minority of tunneling membrane nanotubes in normal and cancer urothelial cells. Sci Rep. 8: 17133-.
Xiao, X., Djurisic, M., Hoogi, A., Sapp, R. W., Shatz, C. J., & Rubin, D. L. (2018). Automated dendritic spine detection using convolutional neural networks on maximum intensity projected microscopic volumes. Journal of neuroscience methods, 309, 25-34.
Calvo V., Izquierdo M. (2018). Imaging Polarized Secretory Traffic at the Immune Synapse in Living T Lymphocytes. Front. Immunology 9:684.
Manso, Y., Holland, P. R., Kitamura, A., Szymkowiak, S., Duncombe, J., Hennessy, E., ... & McColl, B. W. (2018). Minocycline reduces microgliosis and improves subcortical white matter function in a model of cerebral vascular disease. Glia, 66(1), 34-46.
Huygens was used for deconvolution.
Tuncay, E., Bitirim, V., Olgar, Y., Durak, A., Rutter, G. A., & Turan, B. (2018). Zn 2+-transporters ZIP7 and ZnT7 play important role in progression of cardiac dysfunction via affecting sarco (endo) plasmic reticulum-mitochondria coupling in hyperglycemic cardiomyocytes. Mitochondrion.
Malagnino, V., Hussner, J., Seibert, I., Stolzenburg, A., Sager, C. P., & zu Schwabedissen, H. E. M. (2018). LST-3TM12 is a member of the OATP1B family and a functional transporter. Biochemical pharmacology, 148, 75-87.
Markus M. Knodel, Arne Nägel, Sebastian Reiter, Andreas Vogel, Paul Targett-Adams, John McLauchlan, Eva Herrmann and Gabriel Wittum. Quantitative Analysis of Hepatitis C NS5A Viral Protein Dynamics on the ER Surface.
Sugiyama, N., Sonay, A. Y., Tussiwand, R., Cohen, B. E., & Pantazis, P. (2018). Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging. Small, 14(8).
Steiner, C., Gevaert, T., Ganzer, R., De Ridder, D., & Neuhaus, J. (2018). Comparative immunohistochemical characterization of interstitial cells in the urinary bladder of human, guinea pig and pig. Histochemistry and cell biology, 1-11.
Zandt B.J., Veruki M.L., Hartveit E., (2018). Electrotonic signal processing in AII amacrine cells: compartmental models and passive membrane properties for a gap junction-coupled retinal neuron. Brain Struct Funct. 2018 Sep;223(7):3383-3410.
Huygens was used for deconvolution, and the Object Stabilizer option was used to "align image slices along the Z-axis to compensate for drift and other mechanical instabilities."
Ashenafi, M. S., & Baroux, C. (2018). Automated 3D gene position analysis using a customized Imaris plugin: XTFISHInsideNucleus. In Plant Chromatin Dynamics (pp. 591-613). Humana Press, New York, NY.
Burdyniuk, M., Callegari, A., Mori, M., Nedelec, F., & Lenart, P. (2018). F-actin patches nucleated on chromosomes coordinate capture by microtubules in oocyte meiosis. bioRxiv, 265009.
Huang, X., Selewa, A., Wang, X., Daddysman, M. K., Gdor, I., Wilton, R., ... & Scherer, N. F. (2018). 3D Snapshot Microscopy of Extended Objects. arXiv preprint arXiv:1802.01565.
Gil-Krzewska, A., Saeed, M. B., Oszmiana, A., Fischer, E. R., Lagrue, K., Gahl, W. A., ... & Krzewski, K. (2017). An actin cytoskeletal barrier inhibits lytic granule release from natural killer cells in patients with Chediak-Higashi syndrome. Journal of Allergy and Clinical Immunology.
Bury L, Coelho PA, Simeone A, Ferries S, Eyers CE, Eyers PA, Zernicka-Goetz M, Glover DM.
Cañas JA1,, Sastre B, Mazzeo C, Fernández-Nieto M, Rodrigo-Muñoz JM, González-Guerra A, Izquierdo M, Barranco P, Quirce S, Sastre J, Del Pozo V.
Carlo Manno, Lourdes C. Figueroa, Dirk Gillespie, Robert Fitts, ChulHee Kang, Clara Franzini-Armstrong, and Eduardo Rios.
This is a very interesting paper on how to improve extremely low signal data with Huygens.
The Huygens software was used to deconvolve and visualize the cover of this issue of The Journal of Biological Chemistry.
Zandt B-J, Liu J.H., Veruki M.L., Hartveit E.
AII amacrine cells: quantitative reconstruction and morphometric analysis of electrophysiologically identified cells in live rat retinal slices imaged with multi-photon excitation microscopy.
This paper describes in much detail the optimimal imaging and deconvolutution settings that were applied.
Huygens was used for deconvolution and the Huygens Colocalization Analyzer option was applied].
Christian A. Ruge, Adam Bohr, Moritz Beck-Broichsitter, Valérie Nicolas, Nicolas Tsapis, Elias Fattal.
Correlated confocal and super-resolution imaging by VividSTORM.
Huygens deconvolution was used to create the cover of this issue of Nature Protocols.
Magalie Bénard, Damien Schapman, Alexis Lebon, Baptiste Monterroso, Marine Bellenger, Frank Le Foll, Jennifer Pasquier, Hubert Vaudry, David Vaudry and Ludovic Galas.
Huygens deconvolution and pixel shift correction was used here.
Unnersjö-Jess D., Scott L., Blom H., Brismar H.
Castilho A., Ambrosio A.F., Hartveit E., Veruki M.L.
Disruption of a Neural Microcircuit in the Rod Pathway of the Mammalian Retina by Duabetes Mellitus.
Brennan F.H., Gordon R, Lao H.W., Biggins P.J., Taylor S.M., Franklin R.J.M., Woodruff T.M., Ruitenberg, M.J.
The Complement Receptor C5aR Controls Acute Inflammation and Astrogliosis following Spinal Cord Injury.
Huygens deconvolution was used to create the cover of this J. Neuroscience issue.
Super-resolution spinning-disk confocal microscopy using optical photon reassignment.
Besseling, T.H., Jose, J. and Blaaderen, A. V.
Methods to calibrate and scale axial distances in confocal microscopy as a function of refractive index.
This paper describes two methods to calibrate Z sampling of 3D confocal microscopes. See also CalibrateAxialDistances.
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer.
This article shows that deconvolution of large datasets like SPIM data, can be easily performed with the Huygens Remote Manager/Core.
Dudok B, Barna L, Ledri M, et al.
H. Peuschel, T. Ruckelshausen, C. Cavelius, A. Kraegeloh.
Quantification of internalized silica nanoparticles via STED microscopy.
Huygens CMLE was used for confocal and STED deconvolution. Huygens Advanced Object Analyzer was applied to segment the cell boundary and to measure the number and geometry of internalized nanoparticles. The authors took the effort to describe the analysis workflow thoroughly.
Cell. 2014 Mar 13;156(6):1274-85. doi: 10.1016/j.cell.2014.01.062.
Schoonover, CE, Tapia, J-C, Schilling VC, Wimmer V, Blazeski R, Zhang W, Mason, CA, Bruno RM (2014), Comparative Strength and Dendritic Organization of Thalamocortical and Corticocortical Synapses onto Excitatory Layer 4 Neurons.
Journal of Neuroscience 14 May 2014, 34 (20) 6746-6758.
With Huygens deconvolution an "adequate x and y resolution was achieved to distinguish spine heads and spine necks".
Koch N, Kobler O, Thomas U, Qualmann B, Kessels M.M. (2014), Terminal Axonal Arborization and Synaptic BoutonFormation Critically Rely on Abp1 and the Arp2/3Complex.
Ting CY, McQueen PG, Pandya N, Lin TY, Yang M, Reddy OV, O'Connor MB, McAuliffe M, Lee CH.
Photoreceptor-derived activin promotes dendritic termination and restricts the receptive fields of first-order interneurons in Drosophila.
Brüggen, B., Meyer, A., Boven, F., Weiler, R. and Dedek, K. (2014), Type 2 wide-field amacrine cells in TH::GFP mice show a homogenous synapse distribution and contact small ganglion cells.
Maturation of late Golgi cisternae into RabE(RAB11) exocytic post-Golgi carriers visualized in vivo.
Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase.
Thauerer B, Voegele P, Hermann-Kleiter N, Thuille N, de Araujo ME, Offterdinger M, Baier G, Huber LA, Baier-Bitterlich G.
LAMTOR2-mediated modulation of NGF/MAPK activation kinetics during differentiation of PC12 cells.
Stimulated Emission Depletion (STED) Microscopy Reveals Nanoscale Defects in the Developmental Trajectory of Dendritic Spine Morphogenesis in a Mouse Model of Fragile X Syndrome.
In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging.
Correlated optical and isotopic nanoscopy.
Fluorescent in situ hybridization of synaptic proteins imaged with super-resolution STED microscopy.
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy.
J. Vis. Exp. (85), e51100, doi:10.3791/51100 (2014).
An Improved Procedure for Subcellular Spatial Alignment during Live-Cell CLEM.
Bhuvanendran, S.; Salka, K.; Rainey, K.; Sreetama, S.C.; Williams, E.; Leeker, M.; Prasad, V.; Boyd, J.; Patterson, G.H.; Jaiswal, J.K.; Colberg-Poley, A.M.
Superresolution Imaging of Human Cytomegalovirus vMIA Localization in Sub-Mitochondrial Compartments.
Poenick S1, Jahnke HG, Eichler M, Frost S, Lilie H, Robitzki AA.
Comparative label-free monitoring of immunotoxin efficacy in 2D and 3D mamma carcinoma in vitro models by impedance spectroscopy.
Biosens Bioelectron. 2014 Mar 15;53:370-6. doi: 10.1016/j.bios.2013.09.048. Epub 2013 Sep 29.
Abrahamsson S, Chen J, Hajj B, Stallinga S, Katsov AY, Wisniewski J, Mizuguchi G, Soule P, Mueller F, Dugast Darzacq C, Darzacq X, Wu C, Bargmann CI, Agard DA, Dahan M, Gustafsson MG.
Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
Nat Methods. 2013 Jan;10(1):60-3. doi: 10.1038/nmeth.2277.
Symes A.J, Eilertsen M, Millar M, Nariculam J, Freeman A, Notara M, Feneley, Patel H.R.H, Masters J.R.W, Ahmed A.
Westhoff J.H, Giselbrecht S, Schmidts M, Schindler S, Beales P.L, Tönshoff B, Liebel U, Gehrig J.
Kreutmayer S, Csordas A, Kern J, Maass V, Almanzar G, Offterdinger M, Öllinger R, Maass M, Wick G.
Chlamydia pneumoniae infection acts as an endothelial stressor with the potential to initiate the earliest heat shock protein.
Coelho PA, Bury L, Sharif B, Riparbelli MG, Fu J, Callaini G, Gloveremail DM, Zernicka-Goetzemail M.
Castañeda V, Cerda M, Santibañez F, Jara J, Pulgar E, Palma K, Lemus CG, Concha M, Hartel S.
Wang XD, Su YA, Wagner KV, Avrabos C, Scharf SH, Hartmann J, Wolf M, Liebl C, Kühne C, Wurst W, Holsboer F, Eder M, Deussing JM, Müller MB, Schmidt MV.
Nectin-3 links CRHR1 signaling to stress-induced memory deficits and spine loss.
Nat Neurosci. 2013 Jun;16(6):706-13. Epub 2013 May 5.
Anje Sporbert, Zoltan Cseresnyes, Meike Heidbreder, Petra Domaing, Stefan Hauser, Barbara Kaltschmidt, Christian Kaltschmidt, Mike Heilemann, Darius Widera.
Alireza Ghamari1, Mariëtte P.C. van de Corput, Supat Thongjuea, Wiggert A. van Cappellen, Wilfred van IJcken,Jeffrey van Haren,Eric Soler,Dirk Eick,Boris Lenhard and Frank G. Grosveld.
In vivo live imaging of RNA polymerase II transcription factories in primary cells.
Lukinavicius G., Umezawa K., Olivier N., Honigmann A., Yang G., Plass T., Mueller V., Reymond L., Corrêa Jr. I.R., Luo Z-G.,	Schultz G., Lemke E.A., Heppenstall P., Eggeling C., Manley S., Johnsson K.
A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.
Lukinavicius G., Lavogina D., Orpinell M., Umezawa K., Reymond L., Garin N., Gönczy P., Johnsson K.
Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex.
Trcek T, Chao J.A., Larson D.R.,Park H.Y., Zenklusen D., Shenoy S.M., Singer R.H.
Single-mRNA counting using fluorescent in situ hybridization in budding yeast.
Malide D, Metais J-Y, Dunbar C.E.
Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by five fluorescent proteins using confocal and multiphoton microscopy.
Micutkova L, Hermann M, Offterdinger M, Hess MW, Matscheski A, Pircher H, Mück C, Ebner HL, Laich A, Ferrando-May E, Zwerschke W, Huber LA, Jansen-Dürr P.
Analysis of the cellular uptake and nuclear delivery of insulin-like growth factor binding protein-3 in human osteosarcoma cells.
Lalle M, Camerini S, Cecchetti S, Sayadi A, Crescenzi M, Pozio E.
Interaction network of the 14-3-3 protein in the ancient protozoan parasite Giardia duodenalis.
Mariëtte P. C. van de Corput, Ernie de Boer, Tobias A. Knoch, Wiggert A. van Cappellen, Adrian Quintanilla,Leanna Ferrand, Frank G. Grosveld.
F Spira, NS Mueller, G Beck, P von Olshausen, J Beig, R Wedlich-Söldner.
Patchwork organization of the yeast plasma membrane into numerous coexisting domains.
Dynamic denitrosylation via S-nitrosoglutathione reductase regulates cardiovascular function.
Spatial variability in T-tubule and electrical remodeling of left ventricular epicardium in mouse hearts with transgenic Gαq overexpression-induced pathological hypertrophy.
POLO ensures chromosome bi-orientation by preventing and correcting erroneous chromosome-spindle attachments.
Trypanosoma cruzi extracellular amastigotes and host cell signaling: more pieces to the puzzle.
Non-canonical signaling of the PTH receptor.
The cover of this issue shows a Huygens deconvolved and alpha blended three-color live endosome.
N Hégarat, E Smith, G Nayak, S Takeda, PA Eyers, H Hochegger.
Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics. Includes cover image that was deconvolved with Huygens.
Lalle M, Rosati MA, Bien J, Hehl AB, Pozio E, Tosini F.
Expression of Cryptosporidium parvum Cpa135/CpCCP1 chimeras in Giardia duodenalis: Organization of the protein domains affects the protein secretion pathway.
Lalle M, Camerini S, Cecchetti S, Blasetti Fantauzzi C, Crescenzi M, Pozio E.
Giardia duodenalis 14-3-3 protein is polyglycylated by a tubulin tyrosin ligase-like member and deglycylated by two metallocarboxypeptidases.
Lalle M, Currà C, Ciccarone F, Pace T, Cecchetti S, Fantozzi L, Ay B, Breton CB, Ponzi M.
Dematin, a component of the erythrocyte membrane skeleton, is internalized by the malaria parasite and associates with Plasmodium 14-3-3.
OA Gorleku, AM Barns, GR Prescott, J Greaves, LH Chamberlain.
Endoplasmic reticulum localization of DHHC palmitoyltransferases mediated by lysine-based sorting signals.
J Greaves, JA Carmichael, LH Chamberlain.
R Peravali, J Gehrig, S Giselbrecht, D Lütjohann, Y Hadzhiev, F Müller, and U Liebel.
Automated feature detection and imaging for high-resolution screening of zebrafish embryos.
S Roizard, C Danelon, G Hassaïne, J Piguet, K Schulze, R Hovius, R Tampé, and H Vogel.
Activation of G-Protein-Coupled Receptors in Cell-Derived Plasma Membranes Supported on Porous Beads.
E Smith, N Hégarat, C Vesely, I Roseboom, C Larch, H Streicher, K Straatman, H Flynn, M Skehel, T Hirota, R Kuriyama and H Hochegger.
Differential control of Eg5-dependent centrosome separation by Plk1 and Cdk1.
L Mensa, G Crespo, MJ Gastinger, J Kabat, S Pérez-del-Pulgar, R Miquel, SU Emerson, RH Purcell, X Forns.
Hepatitis C virus receptors claudin-1 and occludin after liver transplantation and influence on early viral kinetics.
T Feinstein,V Wehbi, J Ardura, D Wheeler, S Ferrandon, T Gardella and J-P Vilardaga.
Retromer terminates the generation of cAMP by internalized PTH receptors.
R Alonso, C Mazzeo, M C Rodriguez, M Marsh, A Fraile-Ramos, V Calvo, A Avila-Flores, I Merida and M Izquierdo.
Diacylglycerol kinase α regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes.
Jeffrey Kamykowski, Peter Carlton, Siddharth Sehgal and Brian Storrie.
coclustering of proteins within platelet α-granules.
Lei Lu NTU Singapore, Mark S. Ladinski and Tomas Kirchhausen.
Vladimir P. Baklaushev, Gaukhar M. Yusubalieva, Eugene B. Tsitrin, Olga I. Gurina, Nadezhda Ph. Grinenko2, Ilya V. Victorov2, and Vladimir P. Chekhonin.
Visualization of Connexin 43-positive cells of glioma and the periglioma zone by means of intravenously injected monoclonal antibodies.
Schlapp G, Scavone P, Zunino P and S Härtel.
Gonzalez D.R., Treuer A.V., Castellanos J., Dulce R.A., and Hare J.M.
Lalle M, Bavassano C, Fratini F, Cecchetti S, Boisguerin P, Crescenzi M, Pozio E.
Involvement of 14-3-3 protein post-translational modifications in Giardia duodenalis encystations.
Lienkamp S, Ganner A, Boehlke C, Schmidt T, Arnold SJ, Schäfer T, Romaker D, Schuler J, Hoff S, Powelske C, Eifler A, Krönig C, Bullerkotte A, Nitschke R, Kuehn EW, Kim E, Burkhardt H, Brox T, Ronneberger O, Gloy J, Walz G.
Greaves J, Gorleku OA, Salaun C, Chamberlain LH.
Palmitoylation of the SNAP25 protein family: specificity and regulation by DHHC palmitoyl transferases.
Q le Duc Q, Q Shi, I Blonk, A Sonnenberg, N Wang, D Leckband, J de Rooij.
Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner.
J Cell Biol. 2010 Jun 28;189(7):1107-15.
Armin H. Seidl, Edwin W Rubel, and David M. Harris. Virginia Merrill Bloedel Hearing Research Center, Department of Otolaryngology–Head and Neck Surgery, University of Washington, Seattle, Washington.
J. Neurosci. 2010, Jan 6;30(1):70-80.
Confined Displacement Algorithm Determines True and Random Colocalization in Fluorescence Microscopy.
Zoltan Cseresnyes, Ulf Schwarz and Catherine M Green.
Oscillatory recruitment of signaling proteins to cell tips promotes coordinated behavior during cell fusion.
Antioxidants & Redox Signaling. January 2009, 11(1): 1-14.
A simple method for multiday imaging of slice cultures.
Microscopy Research and Technique. 2009 Jun 29.
Landeira D, Bart JM, Van Tyne D, Navarro M.
Cohesin regulates VSG monoallelic expression in trypanosomes.
J Cell Biol. 2009 Jul 27;186(2):243-54.
Shack-Hartmann wave front measurements in cortical tissue for deconvolution of large three-dimensional mosaic transmitted light BRIGHTFIELD micrographs.
Lei Lu NTU Singapore, Mark S. Ladinsky and Tom Kirchhausen.
Cisternal Organization of the Endoplasmic Reticulum during Mitosis.
Molecular Biology of the Cell, Vol. 20, 3471–3480, August 1, 2009.
Fidorra M, Garcia A, Ipsen J, Härtel S & L Bagatolli.
Lipid domains in giant unilamellar vesicles and their correspondence with equilibrium thermodynamic phases: A quantitative fluorescence microscopy imaging approach.
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1788(10):2142-2149 (2009).
Swift F, Birkeland JA, Tovsrud N, Enger UH, Aronsen JM, Louch WE, Sjaastad I, Sejersted OM.
Altered Na+/Ca2+-exchanger activity due to downregulation of Na+/K+-ATPase alpha2-isoform in heart failure.
Cardiovasc Res. 2008 Apr 1;78(1):71-8. Epub 2008 Jan 17. Erratum in: Cardiovasc Res. 2008 Jul 15;79(2):352.
Peroxisome fission in Hansenula polymorpha requires Mdv1 and Fis1, two proteins also involved in mitochondrial fission.
Deconvolution allows reliable imaging of living cells at low fluorescence level.
Poster presented at the ELMI 2008 meeting.
Optics Express, Vol. 16, Issue 11, pp.
Alberto Sanchez-Diaz, Vanessa Marchesi, Stephen Murray, Richard Jones, Gislene Pereira1, Ricky Edmondson, Terry Allen & Karim Labib.
Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast.
Wüstner, D. 2008. Free-cholesterol loading does not trigger phase separation of the fluorescent sterol dehydroergosterol in the plasma membrane of macrophages. Chem. Phys. Lipids. 154, 129-136.
Wüstner, D. and Færgeman, N.J. 2008 Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets. Cytometry A. 73, 727-744.
Wüstner, D., and Færgeman, N.J. 2008. Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells. Histochem. Cell Biol. 130 , 891-908.
Yu Zhang, SoHee Kwon, Teppei Yamaguchi, et al.
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Aging associated with mild dyslipidemia reveals that COX-2 preserves dilation despite endothelial dysfunction.
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Nuclear repositioning of the VSG promoter during developmental silencing in Trypanosoma brucei.
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Depletion of membrane cholesterol eliminates the Ca2+-activated component of outward potassium current and decreases membrane capacitance in rat uterine myocytes.
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The Na+/K+-ATPase alpha2-isoform regulates cardiac contractility in rat cardiomyocytes.
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Nuclear architecture underlying gene expression in Trypanosoma brucei.
Trends Microbiol. 2007 Jun;15(6):263-70. Review.
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Evidence that differential packaging of the major platelet granule proteins von Willebrand factor and fibrinogeen can support their differential release. Journal of Thrombosis and Haemostasis July 2007.
Engstler,M., Pfohl,T., Herminghaus,S., Boshart,M., Wiegertjes,G., Heddergott,N., and Overath,P. (2007).
Hydrodynamic flow-mediated protein sorting on the cell surface of trypanosomes.
El-Helou V, Proulx C, Gosselin H, Clement R, Mimee A, Villeneuve L, Calderone A.
Dexamethasone treatment of post-MI rats attenuates sympathetic innervation of the infarct region.
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Platelet secretion: indiscriminately spewed forth or highly iorchestrated?
Hans-Ulrich Dodt, Ulrich Leischner, Anja Schierloh, Nina Jaerling, Christoph Peter Mauch, Katrin Deininger, Jan Michael Deussing, Matthias Eder, Walter Zieglgaensberger, Klaus Becker.
SO, a protein involved in hyphal fusion in Neurospora crassa, localizes to septal plugs.
High resolution confocal microscopy and nano-scale distance measurements show difference in 3D confirmation between active and inactive mouse B-major Globin Loci?
"Huygens Visualization tools were used."
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Bone marrow-derived progenitor cells contribute to lung remodelling after myocardial infarction.
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Huygens Remote Manager, a Web Interface for High-Volume Batch Deconvolution.
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The Level and Integrity of Synaptic Input Regulates Dendrite Structure.
Technology feature Microscopy: Something to see. Light microscopy is undergoing a renaissance, with a huge range of tools and techniques for gathering biological data with unprecedented speed and resolution.
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The Giardia duodenalis 14-3-3 protein is post-translationally modified by phosphorylation and polyglycylation of the C-terminal tail.
Seven transmembrane receptor core signaling complexes are assembled prior to plasma membrane trafficking.
Ilaria Dal Pra, Anna Chiarini, Alessandro Boschi, Guiliano Freddi and Ubaldo Armato.
Novel dermo-epidermal equivalents on silk fibroin- based formic acid-crosslinked three-dimensional nonwoven devices with prospective applications in human tissue engineering/regeneration/repair.
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IQGAP1 stimulates actin assembly through the N-WASP-ARP2/3 pathway.
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Colchitaxel, a coupled compound made from microtubule inhibitors colchicine and paclitaxel.
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Restoring depressed HERG K+ channel function as a mechanism for insulin treatment of abnormal QT prolongation and associated arrhythmias in diabetic rabbits.
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Ilaria Dal Pra, Anna Chiarini, Edward F. Nemeth, Ubaldo Armato.
Roles of Ca(2+)and the Ca(2+)- Sensing Receptor (CARS) in the Expression of Inducible NOS ( Nitric Oxide Synthase)-2 and Its BH4 (Tetrahydrobiopterin)-Dependent Activation in Cytokine-Stimulated Adult Human Astrocytes.
Anna Chianini, Ilaria Dalpra, Lia menapace, Raffaella Pacchiana, James F. Whitfield and Ubaldo Armato.
Solube amyloid beta-peptide and myelin basic protein strongly stimulate, alone and in synergism with combined pro-inflammatory cytokines, the expression of functional nitric oxide synthase-2 in normal adult human astrocytes.
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Ultrafine particles cross cellular membranes by non-phagocytic mechanisms in lungs and in cultured cells.
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This paper nicely shows that quantification of widefield data was more trustworthy after deconvolution.
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"Huygens Visualitzation tools were used"
Untersuchungen zur Anordnung von Makro- und Mikrochromosomen im Zellkern embryonaler Fibroblasten und Neuronen von Gallus domesticus.
Dissertation zum Erwerb des Doktorgrades der Medizin an der Medizinischen Fakultät der Ludwig-Maximilians-Universität zu München.
This paper shows the need to take object orientation into account when segmenting or analyzing microscopic images.

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