Source: https://www.mcgill.ca/dentistry/research/our-researchers/barros
Timestamp: 2019-04-23 16:30:04+00:00

Document:
Proteolytic enzymes (proteases/peptidases) are crucial biological mediators involved in many physiological and pathological processes, where they function to activate or inactivate substrate proteins or peptides. My research activities are centred around proteases, particularly their actions in mineralized tissues (bones and teeth) and in tumors. More specifically, my lab are focuses on the enzyme PHEX, and how the absence of active PHEX causes X-linked hypophosphatemia (XLH) ‒ the most prevalent form of the hereditable hypophosphatemias which have osteomalacia (hypomineralized, soft skeletons and teeth) as their major symptom. We have recently identified osteopontin as the first physiologically relevant protein substrate for PHEX. Finally, my experience as a biochemist and enzymologist allows me to investigate the role of proteases in bone biology and in tumor-associated processes to understand their specificities and inhibition mechanisms.
Keywords: Proteases, PHEX, XLH, cathepsins, bone, mineralization.
McKee, Marc D. ; Hoac, Betty ; Addison, William N. ; Barros, Nilana M.T. ; Millán JL ; Chaussain C . Extracellular matrix mineralization in periodontal tissues: Noncollagenous matrix proteins, enzymes, and relationship to hypophosphatasia and X-linked hypophosphatemia. Periodontology 2000, v. 63, p. 102-122, 2013.
Barros, Nilana MT ; Hoac, Betty ; Neves, Raquel L ; Addison, William N ; Assis, Diego M ; Murshed, Monzur ; Carmona, Adriana K ; McKee, Marc D . Proteolytic processing of osteopontin by PHEX and accumulation of osteopontin fragments in Hyp mouse bone, the murine model of X-linked hypophosphatemia. Journal of Bone and Mineral Research, v. 28, p. 688-699, 2013. Corresponding author.
Scaffa PMC ; Vidal CMP ; Barros, NMT. ; Gesteiro TF ; CARMONA, Ak ; Breschi L ; Pashley DH ; Tjäderhane L ; TERSARIOL, Ivarne Luis ; NASCIMENTO, F. D. ; Carrilho MR . Chlorhexidine Inhibits the Activity of Dental Cysteine pCathepsins. Journal of Dental Research (Online), v. 91, p. 420-425, 2012.
Bagnaresi, Piero ; de Barros, Nilana MT ; Juliano, Maria A ; Fonseca, Raphael G ; Assis, Diego M ; Juliano, Luiz ; Pesquero, João B ; Melo, Pollyana MS ; Gazarini, Marcos L ; Rosenthal, Philip J ; Carmona, Adriana K . Intracellular proteolysis of kininogen by malaria parasites promotes release of active kinins. Malaria Journal (Online), v. 11, p. 156, 2012. Equal contribution.
Elaine de Paula Fiod Costa ; Barros, NMT. ; Coppini, Larissa Pereira ; Neves, Raquel L. ; Carmona, AK ; Fernando Marcondes Penha ; Eduardo Büchele Rodrigues ; Eduardo Dib ; Magalhães Jr, O ; Milton Nunes de Moraes Filho ; Acácio de Souza Lima ; Maia, Mauricio ; Michel Eid Farah . Effects of Light Exposure, pH, Osmolarity, and Solvent on the Retinal Pigment Epithelial Toxicity of Vital Dyes. American Journal of Ophthalmology, p. S0002-9394(12)0, 2012. Corresponding author.
Oliveira, Marcela ; Assis, DM ; Paschoalin T ; MIranda A. ; Ribeiro, E. B. ; Juliano, Maria A. ; Christhofolete, M. A. ; Barros, Nilana M.T. ; Carmona, AK . Cysteine cathepsin S processes leptin, inactivating its biological activity. Journal of Endocrinology, v. 214, p. 217-224, 2012 Corresponding author.
Icimoto, MY ; Barros, Nilana M.T. ; Ferreira, JC ; Marcondes, MF ; Marcelo F Marcondes ; Andrade, D ; Machado, MF ; Juliano, Maria A. ; Júdice, WA ; JULIANO, L ; Oliveira, V . Hysteretic behavior of proprotein convertase 1/3 (PC1/3). Plos One, v. 6, p. 1-7, 2011.
Clara RO, Soares TS, Torquato RJS; Lima CA, Watanabe, ROM, Barros NMT, Carmona AK, Masuda A, Vaz Junior IS, Tanaka A. Boophilus microplus cathepsin L-like (BmCL1) cysteine protease: specificity study using a peptide phage display library (2011) Veterinary Parasitology.
Oliveira M, Torquato RJS, Alves MFM, Juliano MA, Juliano L, Bromme D, Barros NMT, Carmona AK Improvement of cathepsin S detection using fluorescence resonance energy transfer (FRET) peptides based on putative natural substrates (2010) Peptides 31(4):562-7 Corresponding author.
Coppini LP ; Barros, N. M. ; Oliveira M ; Hirata, I. Y. ; Alves, M. F. ; Paschoalin, T. ; Assis Dm ; Juliano, Maria Aparecida ; Puzer, Luciano ; Bromme, D. ; Carmona, AK. Plasminogen hydrolysis by cathepsin S and identification of derived peptides as selective substrate for cathepsin V and cathepsin L inhibitor (2010) Biological Chemistry 391(5):561-70 Equal contribution.

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