Source: http://extracellularvesicles.no/index.php?page=research-production-in-rrnev
Timestamp: 2019-04-19 05:06:45+00:00

Document:
RRNEV has aimed to establish coordinated research projects between the project groups. These projects have been supported by funding from RRNEV. The fundings have been based on evaluation of the projects by all the network groups.
“Implementation and validation of methods for isolation of EVs from plasma”.
Aims: To establish extended use of size exclusion chromatography (SEC) as a standardized preclearing step of plasma samples before EV-specific isolation and downstream characterization. As a part of the project, a bachelor thesis was recently conducted by biomedical laboratory scientist Tine Hiorth Schøyen; “Quantification of specific microRNAs in extracellular vesicles (EVs) from plasma”.
“Urinary extracellular vesicles as source of prostate cancer biomarkers”.
Aims: To develop simple methods for the isolation of urinary extracellular vesicles (EVs) and to identify prostate cancer biomarkers in urinary EVs.
“Extracellular vesicles as potential biomarker of hypoxia-induced rectal cancer aggressiveness”.
Aims: Colorectal cancers, like most solid cancers, contain hypoxic regions that are characterized by oxygen deprivation as a result of differential perfusion of oxygen. Hypoxia is an important hallmark of the tumor environment and a main mechanism both in resistance to therapy and metastatic progression. The overall aim of the project is to investigate extracellular vesicles as an attractive biomarker approach for characterizing the hypoxic phenotype.
“EV profiles of primary cell lines derived from Pancreatic Ductal Adenocarcinoma (PDAC) patients”.
Aims: The overall goal is to evaluate EV profiles of miRNAs as diagnostic and/or prognostic biomarkers in plasma of pancreatic cancer patients as well as in serum of lung cancer patients. The method, when established, will also be used to identify miRNAs in EVs in serum of breast cancer patients.
“Functional microvesicle (MV)-associated tissue factor (TF) activity as a potential biomarker of thrombosis in cancer”.
Aims:To modify and increase the sensitivity of the Calibrated Automated Thrombogram (CAT)-assay for measuring procoagulant activity of TF-bearing microvesicles (MV-TF), and apply the modified method on clinical samples.
Aims:To investigate endocytosis of extracellular microvesicles in non-phagocytic and phagocytic cells and to evaluate the influence of extracellular vesicles on recipient cells after internalization. The project will include a Master thesis project, which will be conducted by Lily Alice Skaaraas (start August 2015).
“Standardization of the use of NanoSight for the characterization of EVs”.
Aims:To standardize NanoSight analyses of selected biological EVs and commercial beads, using three different NanoSight instruments. We aim to evaluate intra- and inter-assay variation of NTA measurements of both size and concentration of different biological EVs and commercially available beads, as well as the effect of selected NTA software settings.
Diagnostic and prognostic relevance of circulating cell-free miRNAs in prostate cancer: a systematic review. E. Endzeliņš, V. Melne, Z. Kalniņa, V. Lietuvietis, U. Riekstiņa, A. Llorenteand A. Linē. Molecular Cancer. 2016. 15:41.
PIKfyve inhibition affects exosome release and secretory autophagy. N.P. Hessvik, A. Øverbye, A. Brech, M.L. Torgersen, I.S. Jakobsen, K. Sandvig and A. Llorente. Cell. Mol. Life Sci. 2016 Jul 20. Epub ahead of print. DOI:10.1007/s00018-016-2309-8.
S. Fais, B. Giebel, G. Camussi, F. Cappello, J. Carvalho, A. Cordeiro da Silva, R. Furlan, V. Iglic, B. Kaeffer, M.E. Lekka, T. Lener, G. Lipps, A. Llorente, A. Marcilla, S. Marei, I. Nazarenko, L. O’Driscoll, C. Oliveira, E. Rohde, H. Vasconcelos, M.H.M. Wauben, M.L. Yliperttula, N. Zarovni, A.B. Zavec. Evidence-based consensus on the use of nanosized extracellular vesicles in nanomedicine. ACS Nano. 2016. 10:3886-99.
A. Øverbye, T. Skotland, C. Koehler, B. Thiede, T. Seierstad, V. Berge, K. Sandvig, A.Llorente. Identification of Prostate Cancer Biomarkers in Urinary Exosomes. Oncotarget. 2015, 6(30):30357-76.
M. Yáñez-Mó, P. Siljander, Z. Andreu, A.B. Zavec, F.E. Borràs, E.I. Buzas, K. Buzas, E. Casal, F. Cappello, J. Carvalho, E. Colás, A. Cordeiro-da Silva, S. Fais, J.M. Falcon-Perez, I.M. Ghobrial, B. Giebel, M. Gimona, M. Graner, I. Gursel, M. Gursel, N.H.H. Heegaard, A. Hendrix, P. Kierulf, K. Kokubun, M. Kosanovic, V. Kralj-Iglic, E. Kramer-Albers, S. Laitinen, C. Lässer, T. Lener, E. Ligeti, A. Line,G. Lipps, A. Llorente, J. Lötvall, M. Mancek-Keber, A. Marcilla, M. Mittelbrunn, I. Nazarenko, E.N.M. Nolte-‘t Hoen, T.A. Nyman, L. O'Driscoll, M. Olivan, C. Oliveira, É. Pállinger, J. Reventós, M. Rigau, M. Sammar, F. Sanchez Madrid, M. Stampe Ostenfeld, R. Stukelj, H.A. del Portillo, E. Rhode, N. Santarém, K. Schallmoser, W. Stoorvogel, S.G. van der Grein, M.H. Vasconcelos, M.H.M. Wauben, O. De Wever. Biological Properties of Extracellular Vesicles and Their Physiological Functions. Journal of Extracellular Vesicles, 2015, 4:27066.
Costa-Silva B, Aiello NM, Ocean AJ, Singh S, Zhang H, Thakur BK, Becker A, Hoshino A, Mark MT, Molina H, Xiang J, Zhang T, Theilen TM, García-Santos G, Williams C, Ararso Y, Huang Y, Rodrigues G, Shen TL, Labori KJ, Lothe IM, Kure EH, Hernandez J, Doussot A, Ebbesen SH, Grandgenett PM, Hollingsworth MA, Jain M, Mallya K, Batra SK, Jarnagin WR, Schwartz RE, Matei I, Peinado H, Stanger BZ, Bromberg J, Lyden D. Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver, Nat Cell Biol. 2015 Jun;17(6):816-26.
S. Phuyal, T. Skotland, N.P. Hessvik, A. Øverby, A. Brech, K. Ekroos, K. Sandvig, A. Llorente. The Ether Lipid Precursor Hexadecylglycerol Stimulates the Release and Changes the Composition of Exosomes Derived from PC-3 cells. J. Biol. Chem. 2015,290:4225-37.
Pedersen, KW., Kierulf, B., Manger, I., Oksvold, MP., Li, M., Vlassov, A., Roos, N., Kullmann and Neurauter, A. Direct Isolation of Exosomes from Cell Culture: Simplifying Methods for Exosome Enrichment and Analysis. Translational Biomedicine, 2015, vol. 6 No. 2:18 (in press).
Pedersen, KW., Kierulf, B., Oksvold, MP., Li, M., Vlassov, A., Roos, N., Kullmann, A and Neurauter, A. Isolation and characterization of exosomes using magnetic beads. BioProbes 71, 2015.
M. P. Oksvold, A. Neurauter and K. W. Pedersen. Magnetic bead-based isolation of exosomes. Methods Mol Biol 2015, 1218, 465-81.
Hellum M, Øvstebø R, Brusletto BS, Berg JP, Brandtzaeg P, Henriksson CE. Microparticle-associated tissue factor activity correlates with plasma levels of bacterial lipopolysaccharides in meningococcal septic shock. Thromb Res. 2014 Mar;133(3):507-14. Epub 2013 Dec 29. PMID: 24423888.
Øvstebø R, Hellum M, Aass HC, Trøseid AM, Brandtzaeg P, Mollnes TE, Henriksson CE. Microparticle-associated tissue factor activity is reduced by inhibition of the complement protein 5 in Neisseria meningitidis-exposed whole blood. Innate Immun. 2014 Jul;20(5):552-60. Epub 2013 Sep 19. PMID: 24051102.
M. P. Oksvold, A. Kullmann, L. Forfang, B. Kierulf, M. Li, A. Brech, A. V. Vlassov, E. B. Smeland, A. Neurauter, K. W. Pedersen. Expression of B-cell surface antigens in subpopulations of exosomes released from B-cell lymphoma cells. Clinical Therapeutics 2014 June 36, 847-862.e1.
Phuyal S, Hessvik NP, Skotland T, Sandvig K, Llorente A. Regulation of exosome release by glycosphingolipids and flotillins. FEBS J. 2014 May;281(9):2214-27.
A. Llorente, T. Skotland, T. Sylvänne, T. Rog, D. Kauhanen, A. Orłowski, I. Vattulainen, K. Ekroos, K. Sandvig. Molecular Lipidomics of Exosomes Released by PC-3 Prostate Cancer Cells. Biochim. Biophys. Acta. Molecular and Cell Biology of Lipids. 2013, 1831:1302–1309.
N. P. Hessvik, K. Sandvig, A. Llorente. Exosomal miRNAs as Biomarkers for Prostate Cancer. Frontiers in Genetics. 2013, Volume 4, Article 36.
Øvstebø R, Aass HC, Haug KB, Trøseid AM, Gopinathan U, Kierulf P, Berg JP, Brandtzaeg P, Henriksson CE. LPS from Neisseria meningitidis is crucial for inducing monocyte- and microparticle-associated tissue factor activity but not for tissue factor expression. Innate Immun. 2012 Aug;18(4):580-91. Epub 2011 Dec 16. PMID: 22180561.
Hellum M, Øvstebø R, Trøseid AM, Berg JP, Brandtzaeg P, Henriksson CE. Microparticle-associated tissue factor activity measured with the Zymuphen MP-TF kit and the calibrated automated thrombogram assay. Blood Coagul Fibrinolysis. 2012 Sep;23(6):520-6. PMID: 22732249.
R. J. Simpson, H. Kalra, H. Ji, P. Altevogt, P. Askenase, X. Breakefield, E. Buzas, G. Camussi, A. Clayton, S. Gabrielsson, Y.S. Gho, R. Graca, H.C. Harsha, A. Hill, G. Jenster, L.S. Kiang, A. Llorente, F.S. Madrid, I. Nazarenko, R. Nieuwland, L.M. Nilsson, A. Pandey, S. Pluchino, T.S. Prasad, L. Rajendran, M. Record, G. Reid, W. Stoorvogel, D. Taylor, C. Thery, M. Vidal, M. Zoeller and S. Mathivanan.Vesiclepedia: A Compendium for Extracellular Vesicles that Enables Continuous Community Annotation. PLoS Biology 2012, 10(12):e1001450.
N. Hessvik, S. Phuyal, A. Brech, K. Sandvig, A. Llorente. Profiling of microRNAs in Exosomes Released from PC-3 prostate Cancer Cells. Biochim. Biophys. Acta. Gene regulatory mechanisms. 2012, 1819(11-12):1154-1163.
K. Sandvig, A. Llorente. Proteomic Analysis of Microvesicles Released by the Human Prostate Cancer Cell Line PC-3. Mol. Cell. Proteomics. 2012, 11(7):M111.012914.
Henriksson CE, Hellum M, Haug KB, Aass HC, Joø GB, Øvstebø R, Trøseid AM, Klingenberg O, Kierulf P. Anticoagulant effects of an antidiabetic drug on monocytes in vitro. Thromb Res. 2011 Nov;128(5):e100-6. Epub 2011 Aug 20. PMID: 21862110.
Aass HC, Øvstebø R, Trøseid AM, Kierulf P, Berg JP, Henriksson CE. Fluorescent particles in the antibody solution result in false TF- and CD14-positive microparticles in flow cytometric analysis. Cytometry A. 2011 Dec;79(12):990-9. Epub 2011 Oct 11. PMID: 21990118.
B. Vestad. Ekstracellulære vesikler som biomarkører. Presented at Bioingeniørkongressen 2016; Oslo, Norway.
T.H.Schøyen. MikroRNA fra ekstracellulære vesikler i plasma som potensielle biomarkører. Presented at Bioingeniørkongressen 2016; Oslo, Norway.
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells (ORAL PRESENTATION). S. Phuyal*, T. Skotland, N. Hessvik, H. Simolin, A. Øverby, A. Brech, R. G. Parton, K. Ekroos, K. Sandvig and A. Llorente.
From basic research to clinical setting: adapting methods for EV culturing, enrichment and analysis (ORAL PRESENTATION). B. Paszkiet and A. Neurauter*.
Identification of optimal culturing conditions for studying exosomes from normoxic and hypoxic colorectal cancer cell lines (ORAL WITH POSTER). N. Sivarajah, A.H. Ree, E. Kalanxhi, K.R. Redalen, K. Risberg*.
Microparticle-associated tissue factor activity is reduced by inhibition of the complement protein 5 in Neisseria meningitidis-exposed whole blood. (ORAL PRESENTATION) Øvstebø R*, Hellum M, Aass HC, Trøseid AM, Brandtzaeg P, Mollnes TE, Henriksson CE.
Proteomic analysis of microvesicles released by the prostate cancer cell line PC-3 (ORAL PRESENTATION). A. Llorente* and K. Sandvig.
Exosomes from hypoxic colorectal cancer (CRC) cells – miRNA cargo as indicator of adverse CRC biology? (POSTER). T. Bjørnetrø*.
Exosomal proteins as prostate cancer biomarkers in urine: From mass spectrometry discovery to antibody-based validation (POSTER). A. Llorente*.
Extracellular vesicles derived from SW480 cancer colon cells are internalized by human primary monocytes but not by lymphocytes (POSTER). B. Vestad*, L.A. Skaaraas, A. Llorente, K.B.F. Haug, R. Øvstebø.
CD9-positive EVs isolated from size exclusion chromatography (SEC)-precleared plasma from patients and healthy individuals reveal microRNA (miRNA) patterns (POSTER).K.B.F. Haug*, T.H. Schøyen, B. Vestad, B. Kierulf,P. Kierulf, K.W. Pedersen, A. Neurauter, E.H. Kure, R. Øvstebø.
Haug, KBF et al (2015): Size exclusion chromatography (SEC)-preclearing of plasma from patient samples facilitates magnetic bead based isolation of CD9 or CD81 expressing circulating EVs (POSTER). K.B.F.Haug*, B.Vestad, B. Kierulf, H.C.D. Aass, P. Kierulf, K.W. Pedersen, E. Kure, A. Neurauter, R. Øvstebø.
From basic research to clinical setting: Adapting methods for EV enrichment and analysis (POSTER). A. Neurauter*, B. Kierulf, A. Kullmann and K. W. Pedersen.
The glycosphingolipid synthesis inhibitor PDMP modulates the composition of exosomes released from PC-3 cells (POSTER). S.Phuyal*, N. P. Hessvik, T. Skotland, K. Sandvig, A. Llorente.
B. Vestad*, A. Llorente, A. Neurauter, S. Phuyal, B. Kierulf, P.Kierulf, M. Hellum, K. B. F. Haug, R. Øvstebø.
Expression of surface antigens in subpopulations of exosomes released into the urine from prostate cancer patients (POSTER). A. Neurauter*, A. Kullmann, K. W. Pedersen, M. Oksvold.
Molecular lipidomics analysis of exosomes released by PC-3 prostate cancer cells: implications for exosome membrane structure (POSTER). A. Llorente*, T. Skotland, T. Sylvanne2, D. Kauhanen2, K. Ekroos and K. Sandvig.
Profiling of microRNAs in exosomes from PC-3 prostate cancer cells (POSTER). N.P. Hessvik*, S. Phuyal, A. Brech, K. Sandvig and A. Llorente.
Magnetic beads for fast and reproducible isolation/characterization of exosomes based on surface protein expression. A. Neurauter*, A. Kullmann, B. Kierulf, M. P. Oksvold and K. W. Pedersen.

References: V. 
 V. 
 V. 
 V. 
 V. 
 V.