Source: https://www.naturalremedy.com/rd/
Timestamp: 2019-04-24 10:39:39+00:00

Document:
Natural Remedies R&D Center caters to both Animal Health Care Product as well as Human Health Care business and provides a platform for developing innovative products.
Natural Remedies R&D Centre is well equipped with latest instruments to ensure and maintain the world class standards of health and safety, improved efficacy and increased productivity.
The Phytochemistry laboratory develops commercially viable processes for the optimum extraction of medicinal plants where active constituents (markers) are known. The plant extracts generated thereof are standardized to contain known amount(s) of biologically active constituent(s). For plants where active / markers are not known, bioactivity guided fractionations are undertaken and active / bio-markers are isolated for the purpose of standardization.
Chromatographic techniques like column, flash, MPLC, Preparative HPLC are employed for isolating constituents. The isolated single chemical entities are thoroughly characterized using the conventional spectroscopic (UV, IR, NMR & Mass spectroscopy) and chromatographic techniques.
In the discovery cycle, for the predictions of the effects that a drug will display, measurement of its specific functional response induced in a biological system ‘a bioassay’ is required. A bioassay is thus an essential tool for research and development. Bioassays based on the in vitro responses of enzymes or stable cell lines are commonly the assay systems of choice for this purpose. These bio-assays are rapid, reproducible, economical, sample hungry.
We have standardized and routinely perform more than 125 in-vitro assays relevant to selection and optimization of new drug candidates using specialized assay technologies based on different detection modes like Absorbance (colorimetric, ELISA), Luminescence and Fluorescence (Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence etc) in 96 and 384 well formats. The simple, rapid and cost-effective nature of bioassays facilitates screening of a large number of samples. Plants effective in in-vitro assays are developed into prototypes, which ultimately get tested in in-vivo assays.
Apart from new product development, bio-assays can be effectively be used for screening campaigns, bio-activity guided fractionation, compatibility studies, and stability studies.
Development of in-vitro pharmacological methods for drug evaluation.
Biological standardization of herbal products.
Application of bioassay methods to stability testing and testing combinational effect of herbal extracts.
This laboratory is specialized to conduct microbiological quality control and antimicrobial screening of herbal products. Various analytical procedures in this laboratory are tailor made to suit herbal products and are in accordance with USP method.
Analysis of herbal products for microbiological contamination.
Immersion bio-autography for identification of antibacterial compounds.
Testing of plant extracts and their derivatives for combinational effects to determine synergy, antagonism and additivity.
The Department is comprised of three major divisions: In vitro, In vivo and Clinical Pharmacology. Presently, the department includes a team of competent scientists from various disciplines like pharmacology, biochemistry, and other allied life sciences. The department is committed to the development of a strong herbal research environment within and between various other divisions. The divisions are well equipped with sophisticated instruments for in vitro pharmacological assays and a central animal facility registered under Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) for efficacy and toxicity studies.
Evaluation of herbs for therapeutic use and safety assessment of mono- and polyherbal formulations are the core areas of focus. The team is engaged in evaluating a range of products for veterinary and human health care, to cater to the needs of livestock and human segments.
High Performance Liquid Chromatograph (HPLC), Preparative & Analytical.
This group is already engaged in development of analytical monographs for more than one dozen Indian Medicinal Plants for the forthcoming Indian Pharmacopoeia along with Central Indian Pharmacopoeia Laboratory (C I P L) (Govt. of India), Ghaziabad. This lab routinely uses specific and non-specific bio-assays wherever possible in order to achieve bio-standardization.
Development of meaningful quality assessment parameters for plants where actives/bioactives are not known.
Quantitative determination of known active principles, biomarkers and marker components in raw material, extracts, essential Oils using HPLC, HPTLC, GC etc.
Development and validation of analytical procedures.
Residual analysis (Heavy metals, Mycotoxins, Pesticides & Solvents).
Formulation and Development is one of the key department in R&D.
The main objective of this department is to develop new formulations in different dosage forms for Human and Veterinary use.
The lab is equipped with basic infrastructure for carrying out formulation studies.
The lab has developed first herbal aerosol spray for veterinary use in India.
Development of appropriate dosage forms which are stable and convenient to administer.
Preformulation studies to understand the nature of actives from formulation angle.
Stability studies of finished formulations for establishing the shelf life.
Conducting scale up trials to produce standard operating procedures for commercial production.
As a manufacturer of herbal products, we consume more than 500 tones of crude drug / annum. This requirement of crude drugs is almost wholly fulfilled by collections from the wild / forests. But it is not possible to derive all this material from the wild, because the population of wild medicinal plants is growing thinner day by day due to over exploitation and indiscriminate deforestation. Moreover, because of insufficient raw material ( poor availability ), the price of material shoots up. These are thus valid reasons for cultivating medicinal plants.
We undertake Contract Farming of selected Indian medicinal plants through interested farmers and offer cent percent buy back. Our agriculture farming is stipulated under strictly defined conditions as are specific for each plant. We provide the planting material and complete cultivation plan to farmers and to supervise the proper implementation of plan we have groomed a team of Agriculture Graduates. Our agronomy team has covered approximately 1500 acres of land under contract farming in South India through buy back agreements.
Through contract farming we have done good R&D work to formulate Good Agricultural Practices, which includes proper selection and identification, propagation methods, cultivation techniques, harvesting, step-wise quality control of raw material up to processing stage, post-harvest treatment, storage and safety.
D’Souza P, Rao A, Amit A (1995) Stability of anti-bacterial activity of Himax.Indian Journal of Indigenous Medicine. 16: 135 – 137.
Vijayalakshmi K (1999) Cultivation of Medicinal Plants & Contract Farming. (Medicinal & Aromatic Plants). Plant Hort Tech 39-41.
Prashanth D, John S (1999) Antibacterial activity of Peganum harmala. Fitoterapia 70: 438 - 439.
Prashanth D, Asha MK, Amit A (2001) Antibacterial activity of Punica granatum. Fitoterapia 72: 171-173.
Prashanth D, Padmaja R, Samiulla DS (2001) Effect of certain plant extracts on alpha-amylase activity. Fitoterapia 72: 179-181.
Samiulla DS, Prashanth D, Amit A (2001) Mast cell stabilizing activity of Bacopa monnieri. Fitoterapia 72: 284-285.
Prashanth D, Asha MK, Amit A, Padmaja R (2001) Anthelmintic Activity of Butea monosperma. Fitoterapia 72: 421-422.
Asha MK, Prashanth D, Padmaja R, Amit A, Murali B (2001) Anthelmintic activity of essential oil of Ocimum sanctum & eugenol. Fitoterapia 72: 669-670.
Prashanth D, Amit A, Samiulla DS, Asha MK, Padmaja R (2001) Alpha -glucosidase inhibitory activity of Mangifera indica. Fitoterapia 72: 686-688.
Samiulla DS, Prashanth D, Amit A, Venkataraman BV (2001) Trypsin inhibitory activity of Punica granatum. Linn Journal of Natural Remedies 1: 73-74.
Samiulla DS, Harish MS (2001) Comparative effect of NR-AG-I (Stonil) and NR-AG-II (Stonil) (polyherbal formulations) against gentamicin-induced nephrotoxicity in rats. Journal of Natural Remedies. 1: 42 - 44.
Murali B, Amit A, Anand MS, Dinesh TK, Samiulla DS (2001), An improved HPLC method for estimation of phyllanthin and hypophyllanthin in Phyllanthus amarus. Journal of Natural Remedies. 1: 55-59.
Arun PC, Murali B, Anand MS, Deepak M (2001) Screening of pyrrolizidine alkaloids in some herbal drugs. Journal of Natural Remedies. 1: 67-69.
Nadig PD, Nanjraje U, Amit A (2001) In vitro uterotonic activity of Involon. study Journal of Natural Remedies 1: 158 – 159.
Rajeshwari NR, Pande CB, Kiran A (2001) Effect of Zeestress on broiler performance-a study Journal of Natural Remedies. 1(1): 70-72.
Prashanth D, Deepak M, Padmaja R, Sandhya KV, Anjana M, Arun PC, Amit A (2002) Brine shrimp lethality assay of Bacopa monnieri. Phytotherapy Research 16: 197 - 198.
Padmaja R, Arun PC, Prashanth D, Deepak M, Amit A, Anjana M (2002) Brine shrimp lethality bioassay of selected Indian Medicinal plants. Fitoterapia 73: 508-510.
Yogisha S, Samiulla DS, Prashanth D, Padmaja R, Amit A (2002). Trypsin inhibitory activity of Lawsonia inermis. Fitoterapia73: 690-691.
Deepak M, Dipankar G, Prashanth D, Asha MK, Amit A, Venkataraman BV (2002) Tribulosin and Alpha-sitosterol – D-glucoside, the anthelmintic principles of Tribulus terrestris. Phytomedicine 9: 753-756.
Sudha S, Kumaresan S, Amit A, David J, Venkatraman BV (2002) Anti-convalsant activity of different extracts of Centella asiatica and Bacopa monnieri in animals. Journal of Natural Remedies. 2: 33-41.
Murali B, Amit A, Anand S, Venkataraman BV (2002) An HPLC method for simultaneous estimation of Psoralen, Bakuchicin and Bakuchiol in Psoralea corylifolia. Journal of Natural Remedies. 2: 76-79.
Prashanth D, Asha MK, Balaji G, Biju J, Yogisha S, Amit A (2002) Stability of antimicrobial activity of Wisprec – a cross sectional study. Journal of Natural Remedies 2: 96 – 98.
Ashok K, Amit A, Sujatha M, Murali B, Anand MS (2002) Effect of aging on andrographolide content in Andrographis paniculata Journal of Natural Remedies 2 : 179-181.
Murali B, Amit A, Anand MS, Samiulla DS (2002) Estimation of wedelolactone and demethylwedelolactone in Eclipta alba Hassk by improved chromatograhic analysis. Journal of Natural Remedies 2: 99 – 101.
Samiulla DS, Deepak M, Yogisha S, Arun PC, Keerthi AM, Prashanth D, Amit A, Venkataraman BV. (2003) "Trypsin inhibitory effect of Wedelolactone & Demethylwedelolactone". Phytotherapy Research 17(4): 420-421.
Prashanth D, Amit A, Yogisha S, Padmaja R. (2003) "Demonstration of biological standardization of selected herbal extracts”. Journal of Natural Remedies, 3/2: 166-173.
Asha MK, Prashanth D, Amit A, Rama MB, Murali B. (2003) "Geraniol, the putative anthelmintic principle of Cymbopogon martinii", Phytotherapy Research 17(8): 957.
Vyjayanthi G, Subhashchandra S, Saxena VS, Nadig PD, Venkateshwarlu K, Serene A, Sathyan S, Bagchi D, Kulkarni C, (2003) “Randomized, double-blind, placebo-controlled trial of Aller-7 in patients with allergic rhinitis, Research Communications in Pharmacology and Toxicology Vol. 8, Issue. 1-2,. Emerging drugs Vol II, IV 15-IV 24.
Amit A, Saxena VS, Pratibha N, Bagchi M, Bagchi D, Stohs SJ. (2003). “Safety of a novel botanical extract formula for ameliorating Allergic rhinitis”, Toxicology Mechanisms and Methods, 13(4), Pg. 253-261.
Amit A, Saxena VS, Pratibha N, D’Souza P, Bagchi M, Bagchi D, Stohs SJ. (2003) “Mast cell stabilization, Lipoxygenase inhibition, hyaluronidase inhibition, anti-histaminic and anti-spasmodic activities of Aller-7, a novel botanical formulation for Allergic rhinitis”. Drugs Under Experimental and Clinical Research. XXIX (3) Pg. 107-115.
Deepak, G.K. Sangli, P.C.Arun and Amit A. (2005) “Quantitative Determination of the Major Saponin Mixture Bacoside A in Bacopa monnieri by HPLC” Phytochemical Analysis 16 : 24-29.
Amit A, Joshua AJ, Saxena VS, Bagchi M, Bagchi D (2005) “Safety evaluation of a novel polyherbal formulation for ameliorating allergic rhinitis” Part II. Toxicology Mechanisms & Methods 15:193-204.
Sudha S, Bindu R, Joyce G, Amit A, Venkataraman BV (2005) “Pharmacological interaction of Centella asiatica and Bacopa monnieri with antiepileptic drugs – an experimental study in rats” Journal of Natural Remedies. Vol 5: 63-69.
Nadig PD, Lakshmi S, (2005) “Study of anti-tussive activity of Ocimum sanctum in guinea pigs” Indian Journal of Physiology and Pharmacology 49(2): 243-245.
Joshua AJ, Venkateshwarlu K, Anuradha K, Amit A (2006) “Current scenario in discovery and development of phytomedicines.” Journal of Indian Veterinary Association 4(2): 34-36.
Damodaran A, Wasim P, Joshua AJ, Amit A (2006) “Prospects and challenges in development of herbal medicines.” Journal of Indian Veterinary Association 4 (2): 39-41.
Singh SK, Khanam S, Shivananda BG, Amit A, Shivananda TN (2006) “Genetic variations in gymnemic acid content of Gymnema sylvestre leaves”, Biomed 1(1): 26-28.
Singh SK, Khanam S, Shivananda BG, Amit A, Harish GU, Shivananda TN (2006) “Effect of age leaf on gymnemic acid content Gymnema sylvestre (R. Br) leaves” Biomed July-Sept, 1 (2): 159-160.
Satish H, Dang R, Shivananda BG, Devi K, Rajesh MS, Deepak M, Shivananda TN (2006), “Antiurolithiatic herbal drugs-A review” Biomed July-Sept., 1 (2): 95-119.
Schauss AG, Wu X, Prior RL, Ou B, Huang D, Owens J, Amit A, Jensen G, Hart AN, Shanbrom E (2006) “Antioxidant capacity and other bioactivities of the freeze-dried Amazonian palm berry, Euterpe oleraceae Mart. (Acai)” Journal of Agricultural And Food Chemistry 54(22): 8604-8610.
Joshua AJ, Vinod DR, Pravina K, Suja RS, Goudar KS, Pande CB, Amit A Lipotropic activity of Natcol, a polyherbal formulation in albino wistar rats. Journal of Natural Remedies 6(2): 174-180.
Vinutha B, Prashanth D, Salma K, Sreeja SL, Pratiti D, Padmaja R, Radhika S, Amit A, Venkateshwarlu K, Deepak M, (2007) “Screening of selected Indian medicinal plants for acetylcholinesterase inhibitory activity”, Journal of Ethnopharmacology 109: 359-363.
Kasture SB, Kasture VS, Joshua AJ, Damodaran A, Amit A, (2007) “Nootropic activity of BacoMind™ an enriched photochemical composition from Bacopa monnieri” Journal of Natural Remedies 7(1): 166-173.
Nikhil S, Sudha S, Aruna B, Amarjeeth R, Guido S, Amit A, (2007) “Preliminary evaluation of different components of Bacopa monnieri for laxative effect.” Journal of Natural Remedies 7(1): 174-182.
Pravina K, Ravindra KR, Goudar KS, Vinod DR, Joshua AJ, Wasim P, Venkateshwarlu K, Saxena VS, Amit A (2007) “Safety evaluation of BacoMind™ in healthy volunteers: A phase I study” Phytomedicine 14: 301-308.
Allan JJ, Damodaran A, Desmukh NS, Goudar KS, Amit A, (2007) “Safety evaluation of astandardized phytochemical composition extracted from Bacopa monnieri in Sprague-dawley rats” Food and Chemical Toxicology 45: 1928-1937.
Deb DD, Kapoor P, Dighe RP, Padmaja R, Anand MS, D’Souza P, Deepak M, Murali B, Amit A (2008) In vitro safety evaluation and anticlastogenic effect of BacoMind™ on human lymphocytes. Biomedical And Environmental Sciences 21: 7-23.
Usha PD, Wasim P, Joshua AJ, Geetarani P, Murali B, Mayachari AS, Venkateshwarlu K, Saxena VS, Deepak M, Amit A (2008) BacoMind™: A cognitive enhancer in children requiring individual education programme. Journal of Pharmacology and Toxicology 3(4): 302-310.
D’Souza PCG, Ally N, Amit A, Padmaja R, Das M, Sarma D, (2008) Demonstration of LanthaScreen™ TR-FRET-based nuclear receptor coactivator recruitment assay using PHERA star a multi-detection HTS microplate reader. Indian Journal of Pharmacology, 40(2): 89-90.
Barbhaiya HC, Desai RP, Saxena VS, Pravina K, Wasim P, Geetharani P, Allan JJ, Venkateshwarlu K, Amit A, (2008) Efficacy and tolerability of BacoMind on memory improvement in elderly participants-A double blind placebo controlled study. Journal of Pharmacology and Toxicology, 3(6): 425-434.
Nigam V, Paarakh PM, Murali B, Amit A, (2008) A HPLC method for the quantitative estimation of guggulsterone-E and guggulsterone-Z in Commiphora mukul (Hook. Et. Stocks) Engl. Gum resin. Pharmakine, 1(1): 25-30.
Joshua AJ, Vijayabalaji V, Goudar KS, Sameera N, Amit A. (2008) Safety assessment of some herbal formulations on acute exposure in rats. Indian Journal of Natural Products 24(3), 10-16.
Joshua AJ, Goudar KS, Damodaran A, Sameera N, Amit A. (2008) Acute oral toxicity evaluation of some polyherbal formulations in albino Wistar rats. International Journal of Pharmacology 4(5): 388-392.
Sumanth M, (2009), Study of antistress activity of NR-A2 in active anaphylaxis-induced allergic rats, Asian Journal of Experimental Sciences 23(1): 317-320.
Chandeasekaran CV, Thiyagarajan P, Sundarajan K, Goudar KS, Deepak M, Murali B, Allan JJ, Agarwal A, (2009) Evaluation of the genotoxic potential and acute oral toxicity of standardized extract of Andrographis paniculata (KalmColdTM) Food and Chemical Toxicology 47: 1892–1902.
Joshua AJ, Pore MP, Deepak M, Murali B, Anand MS, Amit A, (2009), Reproductive and Fertility Effects of an Extract of Andrographis paniculata in Male Wistar Rats International Journal of Toxicology, 28,(4): 308-317 .
Kasture S, Vinci S, Ibha F, Puddu A, Marongiu M, Murali B, Pisanu A, Lecca D, Zernig G, Acquas E (2009) Withania somnifera prevents morphine withdrawal-induced decrease in spine density in nucleus accumbens shell of rats: A confocal laser scanning microscopy study. Neurotoxicity Research 16: 343–355.
John S, Nikhil S, Yaswanth J, Bhaskar A, Amit A, Sudha S (2009)Analgesic property of different extracts of Curcuma longa (Linn): an experimental study in animals, Journal of Natural Remedies 9(1): 116-120.
Dey S, Deepak M, Setty M, D’Souza P, Amit A, Sangli GK, (2009), Bioactive caffeic acid esters from Glycyrrhiza glabra. Natural Product Research, 23(18): 1657-1663.
Saxena RC, Singh R, Kumar P, Yadav SC, Negi MPS, Saxena VS, Joshua AJ, Vijayabalaji V, Goudar KS, Venkateshwarlu K, Amit A (2010) A randomized double blind placebo controlled clinical evaluation of extract of Andrographis paniculata (KalmCold™) inpatients with uncomplicated upper respiratory tract infection, Phytomedicine 17,178–185.
Mukherjee M, Bhaskaran N, Srinath R, Shivaprasad HN, Allan JJ, Shekhar D, Amit A (2010), Anit-ulcer and antioxidant activity of GutGard™. Indian Journal of Experimental Biology, 48(3): 269-274.
Joshua AJ, Goudar KS, Sameera N, Kumar GP, Murali B, Dinakar N, Amit A (2010) Safety Assessment of Herbal Formulations, Rumbion™ and Tyrel™ in Albino Wistar Rats. American Journal of Pharmacology and Toxicology 5(1): 42-47.
Bhanumathy M, Harish MS, Shivaprasad HN, Sushma G, (2010), Nootropic activity of Celastrus paniculata seed, Pharmaceutical Biology, 48(3): 324-327.
Chandrasekaran CV, Gupta A, Amit A, (2010) Effect of an extract of Andrographis paniculata leaves on inflammatory and allergic mediators in vitroJournal of Ethnopharmacology, 129: 203-207.
Vijayabalaji V, Allan JJ, Kumar GP, Murali B, Suryanaraya ML, Amit A, (2010), Evaluation of safety of Naturaliv™ liquid and Zist™ liquid on acute oral exposure in rats, Journal of Pharmacology and Toxicology, 5(8): 450-459.
Agarwal A, Allan JJ, (2010), Antifertility effects of herbs: Need for responsible reporting. Journal of Ayurveda & Integrative Medicine, 1(2): 129-131.
Chandrasekaran CV, Sundarajan K, David K, Amit A, (2010), In vitro efficacy and safety of poly-herbal formulation, Toxicology In Vitro, 24(3): 885-897.
Soosamma J, Madhavi T, Bincy R, Dang R, Deepak M, (2010), New compounds from the stem bark of Crataeva nurvala, Indian Journal of Natural Products, 26(3), 16-19.
Mishra NK, Allan JJ, (2010), Evaluation of anti-inflammatory activity and potency of herbal formulation consists of different proportions of Curcuma longa and Boswellia serrata by using Cotton pellet granuloma and xylene induced mice ear edema model. International Journal of PharmTech Reseach, 2(3): 1855-1860.
Morgan Annette, Stevens J, (2010), Does Bacopa monnieri improve memory performance in older persons? results of randomized, placebo-controlled double-blind trial. The Journal of Alternative And Complimentary Medicine, 16(7): 753-759.
Ramakrishnan G, Allan JJ, Goudar K, Amit A, (2011), Comparative evaluation of anti-inflammatory activity of different extracts of Boswellia serrata in wistar albino rats, International Journal of PharmTech Research, 3(1), 261-267.
Chandrasekaran CV, Thiyagarajan P, Deepak HP, Amit A, (2011), In vitro modulation of LPS/calcimycin induced inflammatory and allergic mediators by pure compounds of Andrographis paniculata (King of bitters) extract, International Immunopharmacology, 11(1): 79-84.
Chandrashekaran CV, Deepak HB, Thiyagarajan P, Kathiresan S, Sangli GK, Deepak M, Amit A, (2011), Dual inhibitory effect of Glycyrrhiza glabra (GutGard™) on COX and LOX products. Phytomedicine. 18(4): 278-284.
Chandrasekaran CV, Jothie RE, Kapoor P, Gupta A, Agarwal A, (2011), Optimization of cell-based assays to quantify the anti-inflammatory/allergic potential of test substances in 96-well format. Inflammopharmacology 19 (3): 169-181.
Thiyagarajan P, Chandrasekaran CV, Deepak HB, Agarwal A, (2011), Modulation of lipopolysaccharide-induced pro-inflammatory mediators by an extract of Glycyrrhiza glabra and its phytoconstituents. Inflammopharmacology, 19(4): 235-241.
Sharma SK, Arogya SM, Deepak HB, Amit A, Chandrashekaran CV, (2011), Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in hepG2 cells. Pharmacognosy Magazine, 7(27):229-233.
Chandrasekaran CV, Sundarajan K, Gupta A, Srikanth HS, Edwin J, Agarwal A (2011) Evaluation of the genotoxic potential of standardized extract of Glycyrrhiza glabra (GutGardTM), Regulatory Toxicology and Pharmacology. 61(3):373-380.
Vishal J, Murugananthan G, Deepak M, Vishwanatha GL, Manohar D, (2011), Isolation and standardization of various phytochemical constituents from methanolic extracts of fruit rinds of Punica granatum. Chinese Journal of Medicines, 9(6): 414-420.
Ozolua RI, Adejayan A, Aigbe OP, Uwaya DO, Agarwal A, (2011) Some characteristic relaxant effects of aqueous leaf extract of Andrographis paniculata and andrographolide on guinea pig tracheal rings, Nigerian Journal of Physiological Sciences, 26: 119-124.
Ozolua RI, Okoro AC, Idogun SE, Amit A, (2011), Effects of standardized methanol extract of Andrographis paniculata on guinea pig tracheal cyclooxygenase and rat paw inflammation, Journal of Pharmacy And Bioresource, 8(2): 80-85.
Madhu K, Lohit K, Saji MJ, Kulkarni C, (2011), Evaluation of safety and efficacy of Curcuma longa Standardized extract (NR-INF-02), glucosemine sulphate and combination of two in the treatment of painful knee osteoarthritis: A randomoized, placebo controlled trial, Indian Journal of Pharmacology, 43: Suppl 1, S11.
Saxena RC, Singh R, Kumar P, Negi MPS, Saxena VS, Geetharani P, Allan JJ, Venkateshwarlu K (2012) Efficacy of an extract of Ocimum tenuiflorum (OciBest™) in the management of general stress: A double-blind, placebo-controlled study Evidence-Based Complementary and Alternative Medicine 1-7.
Deepak HB, Chandrasekaran CV, Dethe S, Deepak M, Pandre MK, Jaya B, Agarwal A, (2012), Hepatoprotective and antioxidant activity of standardized herbal extracts, Pharmacognosy Magazine 8(30): 116-123.
Ramakrishnan G, Alla JJ, (2012), Pharmacological evaluation of different extracts of Boswellia serrata on ylene induced mice ear edema, International Journal of Pharmaceutical Research and Development (IJPRD), 4(2), 21-25.
Srirama R, Deepak HB, Senthilkumar U, Ravikanth G, Gurumurthy BR, Shivanna MB, Chandrasekaran CV, Agarwal A, Shaanker RU, (2012), Hepatoprotective activity of Indian Phyllanthus, Pharmaceutical Biology, 50(8):948-953.
Chandrasekaran CV, Vijayalakshmi MA, Prakash K, Bansal VS, Meenakshi J, Amit A, (2012), Review article: Herbal Approach for Obesity Management, American Journal of Plant Science, 3: 1003-1014.
Rai PS, Bellampalli R, Dobriyal RM, Amit A, Satyamoorthy K, Narayana DBA, (2012), DNA barcoding of authentific and substitute samples of herb of the family Asparagaceae and Asclepiadaceae based on the ITS2 region, Journal of Ayurveda & Integrative Medicine, 3(3): 136-140.
Nadig PD, Revankar RR, Dethe SM, Narayanswamy SB, Aliyar MA, (2012), Effect of Tinospora cordifolia on experimental diabetic neuropathy, Indian Journal of Pharmacology, 44(5): 580-583.
Chandrasekaran CV, Murali B, Deepak M, Agarwal A, (2012), In vitro comparative evaluation of non-leaves and leaves extracts of Andrographis paniculata on modulation of inflammatory mediators, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry 11(2): 191-197.
Goswami SK, Kumar PM, Jamwal R, Dethe S, Agarwal A, Naseeruddin IM, (2012), Screening for Rho-kinase 2 inhibitory potential of Indian medicinal plants used in management of erectile dysfunction, Journal of Ethnopharmacology 144(3): 483-489.
Allan JJ, Bhide RM, Agarwal A, (2012), Safety Assessment of Zigbir®: A Polyherbal Formulation in Sprague-Dawley Rats, Journal of Toxicology, 1-10.
Mehla J, Pahuja M, Dethe SM, Agarwal A, Gupta YK, (2012), Amelioration of intracerebroventricular streptozotocin induced cognitive impairment by Evolvulus alsinoides in rats: in vitro and in vivo evidence, Neurochemistry International, 61(7): 1052-1064.
Mishra B, Sarkar D, Srivastava S, Deepthi S, Chetan N, (2013), Functional natural ingredients for irritable bowel syndrome. International Journal of Bioassays, 2(1): 338-340.
Asha MK, Debraj D, Prashanth D, Edwin JR, Srikantha HS, Muruganantham N, Dethe SM, Anirban B, Jaya B, Deepak M, Agarwal A, (2013), In vitro anti-Helicobacter pyroli activity of a flavonoid rich extract of Glycyrrhiza glabra and its probable mechanisms of action, Journal of Ethnopharmacology.145(2): 581-586.
Johnson TS, Agarwal RK, Agarwal A, (2013), Non-timber forest products as source of livelihood option for forest dwellers: role of society, herbal industries and government agencies, Current Science, 104(4), 440-443.
Chandrasekaran CV, Sundarajan K, Edwin JR, Gururaj GM, Deepak M, Agarwal A, (2013), Immune-stimulatory and anti-inflammatory activities of Curcuma longa extract and its polysaccharide fraction. Pharmacognosy Research, 5(2): 71-79.
Deepak M, Amit A, (2013), ‘Bacoside’ B-the need remains for establishing identity. Fitoterapia, 87: 7-10.
Kumar S, Anandakumar, Allan JJ, Venkatesan V, Kumar P, Amit A, (2013), Fertility enhancing effects of Hatch UP: a herbal formulation in male rats, Journal of Applied Animal Research, 41(4):455-461.
Chandrasekaran CV, SrikanthaHS, Anand MS, Allan JJ, Viji MM, Amit A, (2013), Evaluation of the mutagenic potential and acute oral toxicity of standardized extract of Ocimum sanctum (OciBest™). Human and Experimental Toxicology 32(9): 992-1004.
Puram S, Suh HC, Kim SU, Bharathi B, Joshua AJ, Amit A, Venkatesh K, (2013), Effect of GutGard in the Management of Helicobacter pylori : A Randomized Double Blind Placebo Controlled Study, Evidence-Based Complementary and Alternative Medicine, Article ID 263805, 8 pages, 2013. org/10.1155/2013/263805.
Koteshwar P, Raghavendra KR, Allan JJ, Goudar KS, Venkateshwarlu K, Amit A, (2013), Effect of NR- Salacia on post-prandial hyperglycemia: A randomized double blind, placebo-controlled crossover study in healthy volunteers, Pharmacognosy Magazine, 9(36), 344-349.
Mohan CG, Deepak M, Viswanatha GL, Savinay G, Hanumantharaju V, Rajendra CE, Halemani PD, (2013), Anti-oxidant and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica, Asian Pacifica Journal of Tropical Medicine: 311-314.
Gupta S, Patil R, Reddy N, Prashanth D, Agarwal RK, Amit A, (2013), Need to strengthen herbal veterinary sector, Pharma Times, 45(9): 45-47.
Sasidharan SR, Allan JJ, Senthilkumar A, Vijayabalaji V, Madhavan CAN, Amit A, (2013), An experimental approach for selecting appropriate rodent diets for research studies on metabolic disorders, BioMed Research International, Article ID 752870, 9pages, doi.org/10.1155/752870.
Chandrasekaran CV, Rao BS, Srikanth HS, Edwin JR, Allan JJ, Murali B, Amit A, (2013), Safety evaluation of Turmeric polysaccharide extract: Assessment of mutagenicity and acute oral toxicity. Biomed Research International. 1-11, article ID 158348.
Bagad AS, Joshuallan AJ, Natarajan B, Amit A, (2013), Comparative Evaulation of Anti-Inflammatory Activity of Curucminoids, Turmerones, and aqueous Extract of Curcuma longa. Advances in Pharmacological Sciences, 2013: 1-8 article ID 805756.
Thyagaraj VD, Rjison K, Monica, Anand MS, Laxman PS, Murali B, (2013), A validated RP-HPLC-UV/DAD method for simultaneous quantitative determination of rosmarinic acid and eugenol in Ocimum sanctum Pharmaceutical Methods 4:1-5.
Chandrasekaran CV, Amit A, Mookambesearan V. (2013), Effect of Nelumbo nucifera Petal Extracts on Lipase, Adipogenesis, Adipolysis, and Central Receptors of Obesity. Evidence-Based Complementary and Alternative Medice. 1-8, article ID 145925.
Shinde AS, Shete SM, Prashanth D’Souza, (2014), Evaluation of a polyherbal Aerosaol Formulation as Supportive Treatment for Clinical Mastitis in Dairy Cows. Indian Veterinary Journal. 91(2): 41-42.
Sasidharan SR, Joshua AJ, Senthilkumar A, Vijayabalaji V, Madhavan CAN, Amit A. (2014), Ameliorative potential of Tamarindus indica on high fat diet induced nonalcoholic fatty liver disease in rats. The Scientific World Journal. Article ID 507197, pp 1-10.
Deepak M, Sawant LP, Koshy R, Ankuri P, Singh V, Mayachari A, Sharaf MHM, Murali B, Amit A. (2014), Development and validation of high performance liquid chromatography method for simultaneous estimation of flavonoid glycosided in Withania somnifera aerial parts. ISRN Analytical Chemistry. Article ID 351547, pp 1-7.
Dave UP, Dingankar SR, Saxena VS, Joseph JA, Bethapudi B, Agarwal A, Kudiganti V. (2014), An Open-label Study to Elucidate the Effects of Standardized Bacopa monnieri Extract in the Management of Symptoms of Attention-deficit Hyperactivity Disorder in Children. Advances in Mind-Body Medicine 28(2):10-15.
Senthilkumar A, Joshua AJ, Bharathi B, Amit A. (2014) Anti-inflammatory effects of Turmeric ( Curcuma longa) extracts on acute and chronic inflammation models. Journal of Korean Society Food Science and Nutrition. 43(1): 612-617.
Santhan P. (2014), Leaf structural characteristics of important medicinal plants. International Journal of Research in Ayurveda and Pharmacy. 5(6): 673-679.
Joseph JA, Ayyappan UP, Sasidharan SR, Mutyala S, Goudar KS, Agarwal A (2014), Ameliorative effect of Phytocee™ Cool against carbon tetrachloride-induced oxidative stress. Pharmacognosy Research. 6(4): 320-325.
Wahab Sab BA, Jacob J, Manjunath GG, Singh VK, Mundkinajeedu D, Shankarappa S. (2015), Cycloolivil, a lignan from the roots of Stereospermum suaveolens. Pharmacognosy Research. Jan-Mar; 7(1):45-8.
Illuri R, Bethapudi, Anandakumra S, Murugan S, Joshua JA, Mundkinajeddu D, Agarwal A, Chandrasekaran CV. (2015), Anti-inflammatory activity of polysaccharide fraction of Curcuma longa (NR-INF-02). Anti-inflammatory & Anti-allergy Agents in Medicinal chemistry. 14(1): 53-62.
Selvam R, Sureshbabu G, Saravanakumar M, Prashanth D’Souza (2015), Evaluation of ayurvedic veterinary medicine, Tyrel for treatment of bloat in ruminants. International Journal of Agricultural Sciences and Veterinary Medicine. 3(2): 64-70.
Raina P, Chandrasekaran CV, Aggarwal A, Wagh N, Kaulghanekar R (2015), Comparative analysis of anti-inflammatory activity of aqueous and methanolic extracts of cassia and C. zeylanicum in RAW264.7, SW1353 and primary chondrocytes. International Journal of Pharmacy and Pharmaceutical Sciences. 7(11): 392-396.
Sawant L, Singh VK, Dethe S, Bhaskar A, Balachandran J, Deepak M, Amit A (2015), Aldose reductase and protein tyrosine phosphatase 1B inhibitory active compounds from Syzygium cumini Pharmaceutical Biology. 53(8): 1176-1182.
Jothie Richard E, Illuri R, Bethapudi B, Anandhakumar S, Bhaskar A, Chinampudur Velusami C, Mundkinajeddu D, Agarwal A. (2016), Anti-stress activity of Ocimum sanctum: Possible effects on hypothalamic-pituitary-adrenal axis. Phytotherapy Research. 30(5): 804-815.
Raina P, Chandrasekaran CV, Deepak, Agarwal A, Ruchika KG (2015), Evaluation of subacute toxicity of methanolic/aqueous preparation of aerial parts of sanctum in Wistar rats: Clinical, haematological, biochemical and histopathological studies, Journal of Ethnopharmacology 175: 509-517.
Kumar R, Gupta YK, Singh S, Arunraja S (2015), Cissus quadrangularis attenuates the adjuvant induced arthritis by down regulating pro-inflammatory cytokine and inhibiting angiogenesis. Journal of Ethnopharmacology. 175: 346-355.
Kumar R, Gupta YK, Singh S, Arunraja S (2016), Picrorhiza kurroa inhibits experimental arthritis through inhibition of Pro-inflammatory cytokines, angiogenesis and MMPs. Phytotherapy Research. 30: 112-119.
Kumar R, Gupta YK, Singh S, Arunraja S (2016), Anti-inflammatory Effect of Picrorhiza kurroain Experimental Models of Inflammation. Planta Medica, DOI: 10.1055/s-0042-106304.
Verma S, Deepak M, Amit A, Chatterjee SS, Kumar V (2016), Stress resistance promoting potentials of turmeric oil and curcuminoids in mice. Oriental Pharmacy and Experimental Medicine. 16(4): 1-10.
Kumar R, Nair V, Gupta YK, Singh S, Arunraja S (2016), Berberis aristata Ameliorates adjuvant-induced arthritis by inhibition of NF-kB and activating nuclear factor-E2-related factor 2/hem oxygenase (HO)-1 signaling pathway. Immunological Investigations. 45(6): 473-489.
Gururaj GM, Deepak M, Senthil K, Allan J, Shekhar D, Amit A (2016), Cholesterol lowering potentials of a blend of standardized methanol extracts of Moringa oleifera leaves and fruits in albino wistar rats. International Journal of Pharmacy and Pharmaceutical Sciences. 8(11): 262-268.
Raina P, Deepak M, Chandrasekaran CV, Amit A, Wagh N, Ghanekar RK. (2016), Comparative analysis of anti-inflammatory activity of aqueous and methanolic extracts of Ocimum basilicum (basil) in RAW264.7, SW1353 and human primary chondrocytes in respect of the management of osteoarthritis. Journal of Herbal Medicine 6: 28-36.
Asha MK, Debraj D, Dethe S, Bhaskar A, Muruganantham N, Deepak M, (2017), Effect of flavonoid-rich extract of Glycyrrhiza glabra on gut-friendly microorganisms, commercial probiotic preparations, and digestive enzymes. J Diet Suppl. 14(3): 323-333.
Patil D,Gautam M, Jadhav U, Mishra S, Karupothula S, Gairola S, Jadhav S, Patwardhan B (2010), Physicochemical stability and biological activity of Withania somnifera extract under real-time and accelerated storage conditions. Planta Medica 76(5):481-488.
Rajesh BV, Fleming A, Devada S, Ranvir R, Sundar R. (2013), Anti-psoriatic effect of Tribulus terrestris extract by topical application in mouse model of contact dermatitis. International Journal of Veterinary Science. 2(1): 7-11. (Sample gifted).
Langstieh AJ, Verma P, Thakur AK, Chatterjee SS, Kumar V. (2014) Desensitization of mild stress triggered responses in mice by a Brassica juncea leaf extract and some ubiquitous secondary plants metabolites. Pharmacologia 5(9): 326-338. (Support given).
Thankur AJ, Chatterjee SS, Kumar V. (2014) Antidepressant like effects of Brassica juncea leaves in diabetic rodents. Indian Journal of Experimental Biology 52:613-622. (Support given).
Thakur AK, Chatterjee SS, Kumar V. (2014) Therapeutic potential of traditionally used medicinal plant Andrographis paniculata (Burm. F.) against diabesity: An experimental study in rats. TANG: Humanitas Traditional Medicine, 4(1): e7. (Sample given).
Thakur AK, Soni UK, Rai G, Chatterjee SS, Kumar V. Protective effects of Andrographis paniculata extract and pure andrographolide against chronic stress-triggered pathologies in rats. Cell Mol Neurobiol. 2014, 1-11. (Sample given).
Thakur AK, Chatterjee SS, Kumar V. Antidepressant-like activity of Andrographis paniculata in type – 2 diabetic rats. Clinic Pharmaco Biopharmaceut 2014, S2, 1-9. (Sample given).
Peana AT, Muggironi G, Spina L, Rosas M, Kasture SB, Cotti E, Acquas E, (2014), Effects of Withania somnifera on oral ethanolself-administration in rats, Behaviroal Pharmacology, 25(4): 618-628. (Sample given).
Lu K, Basu S (2015), The natural compound chebulagic acid inhibits vascular endothelial growth factor A mediated regulation of endothelial cell functions. Sci Rep. 5:9642. (Sample given).
Thankur AK, Rai G, Chatterjee SS, Kumar V. (2015), Analgesic and anti-inflammatory activity of Andrographis paniculata and andrographolide in diabetic rodents. E C Pharmaceutical Sciences. 1(1): 19-28. (Sample given).
Durg S, Veerapur VP, Thippeswamy BS, Ahmed SM (2015), Antiepileptic and antipsychotic acitivities of satandardized Silajatu ( Shilajit) in experimental animals. Ancient Science of Life. 35(2): 110-117. (Sample given).
Shakya A, Soni UK, Rai R, Chatterjee SS, KumarV, (2016), Gastro-protective and Anti-stress Efficacies of Monomethyl Fumarate and a Fumaria indica Extract in Chronically Stressed Rats. Cell Mol Neurobiol. 36(4):621-635. (Sample given).
De Rose F, Marotta R, Poddighe S, Talani G, Catelanji T, Setzu MD, Solla P, Marrosu F, Sanna E, Kasture S, Acquas E, Liscia A. (2016), Functional and morphological correlates in the Drosophila LRRK2 loss-of-function model of Parkinsons disease: Drug effects of Withania somnifera (Dunal) administration. PlosOne. 11(1): 1-16.
The Phytochemistry laboratory develops commercially viable processes for the optimum extraction of medicinal plants where active constituents (markers) are known. The plant extracts generated thereof are standardized to contain known amount(s) of biologically active constituent(s). For plants where active / markers are not known, bioactivity guided fractionations are undertaken and active / bio-markers are isolated for the purpose of standardization. Chromatographic techniques like column, flash, MPLC, Preparative HPLC are employed for isolating constituents. The isolated single chemical entities are thoroughly characterized using the conventional spectroscopic (UV, IR, NMR & Mass spectroscopy) and chromatographic techniques.
Formulation and Development is one of the key department in R&D. The main objective of this department is to develop new formulations in different dosage forms for Human and Veterinary use. The lab is equipped with basic infrastructure for carrying out formulation studies. The lab has developed first herbal aerosol spray for veterinary use in India.
As a manufacturer of herbal products, we consume more than 500 tones of crude drug / annum. This requirement of crude drugs is almost wholly fulfilled by collections from the wild / forests. But it is not possible to derive all this material from the wild, because the population of wild medicinal plants is growing thinner day by day due to over exploitation and indiscriminate deforestation. Moreover, because of insufficient raw material ( poor availability ), the price of material shoots up. These are thus valid reasons for cultivating medicinal plants. We undertake Contract Farming of selected Indian medicinal plants through interested farmers and offer cent percent buy back. Our agriculture farming is stipulated under strictly defined conditions as are specific for each plant. We provide the planting material and complete cultivation plan to farmers and to supervise the proper implementation of plan we have groomed a team of Agriculture Graduates. Our agronomy team has covered approximately 1500 acres of land under contract farming in South India through buy back agreements. Through contract farming we have done good R&D work to formulate Good Agricultural Practices, which includes proper selection and identification, propagation methods, cultivation techniques, harvesting, step-wise quality control of raw material up to processing stage, post-harvest treatment, storage and safety.

References: V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V.