Source: https://medicine.dp.ua/index.php/med/article/view/456
Timestamp: 2019-04-21 04:26:30+00:00

Document:
Keywords: antimicrobial activity; herbal medicinal remedies; probiotics; periodontal diseases.
Developed experimental assays enable us to compare the antimicrobial activity of herbal medicinal drugs on Lactobacillus and Bacillus strains probiotics, which have been claimed to possess the ability of suppressing the growth of various oral pathogens. In the treatment of periodontal disease it is advisable to use a comprehensive approach which would include the application of herbal remedies and probiotics. The combination of such effects may be a new approach in dentistry due to their complementary antimicrobial activity. In this study, we researched antimicrobial effects of herbal medicinal drugs (tinctures of some medical plants, solutions Rotocanum and Chlorophyllipt) against collection strains and clinical strains isolated from the oral cavity of patients with periodontitis, and probiotic strains Bacillus subtilis UKM В-5007 and Bacillus licheniformis UKM В-5514 that are part of the active base of probiotic Biosporin (Ukraine), Bacillus clausii from the probiotic Normaflore (Hungary), as well as the strains Lactobacillus spp. – from probiotic Lactobacterinum (Biopharma, Ukraine). For investigation, the standard agar dilution method was used in modification with glass cylinders. The results of the research showed that among the studied herbal medicinal remedies, tinctures of Eucalypthus viminalis, Mentha piperita and Chlorophyllipt had the strongest antimicrobial activity. Probiotic strains are also sensitive to herbal tinctures (except the tinctura of wormwood), which indicate the possibility of only consecutive usage (with an interval of time) of herbal remedies and probiotics in combination therapy in the treatment of periodontal diseases.
Agarwal, V., Lal, P., & Pruthi, V. (2008). Prevention of Candida albicans biofilm by plant oils. Mycopathologia, 165(1), 13–19.
Alok, A., Singh, I. D., Singh, S., Kishore, M., Jha, P. C., & Iqubal, M. A. (2017). Probiotics: A new era of biotherapy. Advanced Biomedical Research, 6(1), 31.
Ashour, H. M. (2008). Antibacterial, antifungal and anticancer activities of volatile oils and extracts from stems, leaves, and flowers of Eucalyptus sideroxylon and Eucalyptus torquata. Cancer Biology and Therapy, 7(3), 399–403.
Astani, A., Reichling, J., & Schnitzler, P. (2009). Comparative study on the antiviral activity of selected monoterpenes derived from essential oils. Phytotherapy Research, 24(5), 673–679.
Bachir, R. G., & Benali, M. (2012). Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pacific Journal of Tropical Biomedicine, 2(9), 739–742.
Carretto, C. F. P., Junqueira, J. C., Almeida, R. B. A., Furlan, M. R., & Jorge, A. O. C. (2010). Antimicrobial activity of Mentha piperita L. against Candida spp. Brazilian Dental Science, 13(1/2), 4–9.
Cermelli, C., Fabio, A., Fabio, G., & Quaglio, P. (2007). Effect of eucaliptus essential oil on respiratory bacteria and viruses. Current Microbiology, 56(1), 89–92.
Cha, J.-D. (2007). Chemical composition and antibacterial activity against oral bacteria by the essential oil of Artemisia iwayomogi. Journal of Bacteriology and Virology, 37(3), 129–136.
Curtis, M. A. (2014). Periodontal microbiology – The lid’s off the box again. Journal of Dental Research, 93(9), 840–842.
Efstratiou, E., Hussain, A. I., Nigam, P. S., Moore, J. E., Ayub, M. A., & Rao, J. R. (2012). Antimicrobial activity of Calendula officinalis petal extracts against fungi, as well as Gram-negative and Gram-positive clinical pathogens. Complementary Therapies in Clinical Practice, 18(3), 173–176.
Erel, Ş. B., Yavaoğlu, N. Ü. K., & Zeybek, U. (2007). Antimicrobial activities of six artemisia species of West Anatolia. Planta Medica, 73(9), 131.
Gazim, Z. C., Rezende, C. M., Fraga, S. R., Estivaleti Svidzinski, T. I., & Garsia Cortez, D. A. (2008). Antifungal activity of the essential oil from Calendula officinalis L. (Asteraceae) growing in Brazil. Brazilian Journal of Microbiology, 39(1), 61–63.
Hleba, L., Kompas, M., Hutková, J., Rajtar, M., Petrová, J., Čuboň, J., Kántor, A., & Kačániová, M. (2016). Antimicrobial activity of crude ethanolic extracts from some medicinal mushrooms. Journal of Microbiology, Biotechnology and Food Sciences, 5(1), 60–63.
Kačániováa, M., Vukovic, N., Horska, E., Salamov, I., Bobkova, A., Hleba, L., Mellen, M., Vatlak, A., Petrova, J., & Bobko, M. (2014). Antibacterial activity against Clostridium genus and antiradical activity of the essential oils from different origin. Journal of Environmental Science and Health, Part B, 49(7), 505–512.
Massiha, A., Majid Khoshkholgh-Pahlavian, M., Issazadeh, K., Bidarigh, S., & Zarrabi, S. (2013). Antibacterial activity of essential oils and plant extracts of Artemisia (Artemisia annua L.) in vitro. Zahedan Journal of Research in Medical Sciences, 15(6), 14–18.
Mishra, R., Tandon, S., Rathore, M., & Banerjee, M. (2014). Antimicrobial and plaque inhibitory potential of herbal and probiotic oral rinses in children: A randomized clinical trial. Indian Journal of Dental Research, 25(4), 485–492.
Moslemi, H. R., Hoseinzadeh, H., Badouei, M. A., Kafshdouzan, K., & Fard, R. M. (2012). Antimicrobial activity of Artemisia absinthium against surdical wounds infected by Staphylococcus aureus in a rat model. Indian Journal of Microbiology, 52(4), 601–604.
Nikolić, M., Glamočlija, J., Ćirić, A., Marković, T., Marković, D., Perić, T., & Soković, M. (2013). Hemijski sastav i antimikrobna aktivnost etarskog ulja pitome nane (Mentha piperita L.). Lekovite Sirovane, 33, 63–72.
Pramila, D. M., Xavier, R., Marimuthu, K., Kathiresan, S., Khoo, M. L., Senthilkumar, M., Sathya, K., & Sreeramanan, S. (2012). Phytochemical analysis and antimicrobial potential of methanolic leaf extract of peppermint (Mentha piperita: Lamiaceae). Journal of Medicional Plants Research, 6(2), 331–335.
Radulovic, N. S., Blagojevic, P. D., Stojanovic-Radic, Z. Z., & Stojanovic, N. M. (2013). Antimicrobial plant metabolites: Structural diversity and mechanism of action. Current Medicinal Chemistry, 20(7), 932–952.
Rios, J. L., & Recio, M. C. (2005). Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology, 100, 80–84.
Rasooli, I., Shayegh, S., & Astaneh, S. D. A. (2009). The effect of Mentha spicata and Eucalyptus camaldulensis essentisl oils on dental biofilm. International Journal of Dental Hygiene, 7(3), 196–203.
Rivis, O. Y., Krivtsova, M. V., Nikolaichuk, V. I., Semenova, G. M., & Barani, E. A. (2012). Mikroflora rotovoi' porozhnyny ljudej z zapal'nymy zahvorjuvannjamy tkanyny parodontu v Uzhgorods'komu rajoni [Microflora of the oral cavity of people with inflammatory diseases of periodontal tissue in Uzhgorod area]. Bulletin of Problems in Biology and Medicine, 1(3), 121–124 (in Ukrainian).
Rivis, O. Y., Krivtsova, M. V., & Nikolaichuk, V. I. (2013). Antagonistic activity of Bacillus probiotics against bacteria isolates of oral cavity of patients with periodontitis. Visnyk of Dnipropetrovsk University. Biology, Medicine, 4(1), 10–13.
Safronova, L. A., Osadchaya, A. I., Avdeyeva, L. V., & Ilyash, V. M. (2009). Vlijanie fitokompozicij na biologicheskuju aktivnost' probioticheskih shtammov Bacillus subtilis. [Influence of phytocompositions on biological activity of Bacillus subtilis probiotic strains]. Likarska Sprava, 3–4, 68–74 (in Russian).
Shalayel, M. H. F., Asaad, A. M., Qureshi, M. A., & Elhussein, A. B. (2017). Anti-bacterial activity of peppermint (Mentha piperita) extracts against some emerging multi-drug resistant human bacterial pathogens. Journal of Herbal Medicine, 7, 27–30.
Shipradeep, K. S., Khare, R. S., Ojha, S., Kundu, K., & Kundu, S. (2012). Development of probiotic candidate in combination with essential oils from medicinal plant and their effect on enteric pathogens: a review. Gastroenterology Research and Practice, 2012, 1–6.
Shulga, L. I. (2011). Fitopreparaty v stomatologii': Suchasnyj stan ta perspektyvy stvorennja [Herbal remedies in dentistry: Current status and perspectives for production]. Clinical Pharmacy, Pharmacotherapy and Medical Standardization, 3–4, 151–156 (in Ukrainian).
Sujana, P., Sridhar, T. M., Josthna, P., & Naidu, C. V. (2013). Antibacterial activity and phytochemical analysis of Mentha piperita L. (Peppermint) – An important multipurpose medicinal plant. American Journal of Plant Sciences, 4(1), 77–83.
Szakiel, A., Ruszkowski, D., Grudniak, A., Kurek, A., Wolska, K. I., Doligalska, M., & Janiszowska, W. (2008). Antibacterial and antiparasitic activity of oleanolic acid and its glycosides isolated from marigold (Calendula officinalis). Planta Medica, 74(14), 1709–1715.
Takahashi, T., Kokubo, R., & Sakaino, M. (2004). Antimicrobial activities of eucalyptus leaf extracts and flavonoids from Eucalyptus maculata. Letters in Applied Microbiology, 39(1), 60–64.
Takarada, K., Kimizuka, R., Takahashi, N., Honma, K., Okuda, K., & Kato, T. (2004). A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiology and Immunology, 19(1), 61–64.
Vorobets, N., & Rivis, O. (2017). Aktual'nist' ta perspektyvy vykorystannja likars'kyh roslyn dlja likuvannja kandydozu rotovoi' porozhnyny [Relevance and perspectives of using medicinal plants for the treatment of oral candidiasis]. Bulletin of Problems in Biology and Medicine, 135, 22–32 (in Ukrainian).
Vorobets, N. M., & Yavorska, H. V. (2016). Modifications of agar diffusion method to determination of the antimicrobial effect of the herbal medicinal products. Ukrainian Biopharmaceutical Journal, 43, 80–84.
Zlatkina, A. R., Isakov, V. A., & Ivanikov, I. O. (2001). Kandidoz kishechnika kak novaja problema gastrojenterologii [Candidiasis of the intestine as a new problem of gastroenterology]. Russian Journal of Gastroenterology, Hepatology, Coloproctology, 6, 33–38 (in Russian).

References: V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V.