Source: https://russianpatents.com/patent/255/2554780.html
Timestamp: 2019-04-25 20:50:18+00:00

Document:
SUBSTANCE: method is based on measuring a flavonoid amount by differential spectral photometry, calculated as Cinaroside at wave length 400 nm, extracting in water and alcohol and using 70% ethanol as an extraction fluid; the total flavonoid amount calculated as Cinaroside and an absolutely dry raw material in percentage (X) is calculated by formula.
EFFECT: method enables measuring the total flavonoid amount as biologically active components having a basic therapeutic action.
The invention relates to chemical-pharmaceutical industry and can be used in the centers of drug quality control and analytical laboratories in the analysis of flavonoids in Choleretic collection No. 3" ("Hitogata No. 3").
The current system of quality control requires continuous improvement approaches to standardization of biologically active compounds using modern methods of analysis and relevant data about their properties, allowing to identify differentially specific group of biologically active compounds.
"Choleretic collection №3" is widely used in modern medicine in the treatment of diseases of the liver and biliary tract (2, 5, 6). The pharmacological action of this drug collection caused by different groups of biologically active compounds. Part of the collection presents chamomile flowers, peppermint leaves, marigold flowers, grass, yarrow, tansy flowers. Designation in the chamomile, peppermint, yarrow and tansy the two most important groups of active ingredients - essential oils and flavonoids suggests the prediction (8). It is known that antispasmodic and anti-inflammatory action of the drug is due to the presence of essential�about oil this choleretic effect is due to a second group of biologically active compounds flavonoids. The presence in the collection of the flowers of calendula enhances anti-inflammatory drug collection (5, 6, 8). According to the normative documentation on "Cholagogue collection No. 3" the definition of quality is the essential oil content (1). Taking into account the fact that the components of essential oils practically insoluble in water while getting the infusion, the content of essential oils in "Cholagogue collection No. 3 does not allow to assess the degree of effectiveness of this drug. In our opinion, in the specified collection is expedient to determine the content of water-soluble active substances - flavonoids, because they determine the main pharmacological action, namely choleretic effect collection (9, 10).
Thus, the aim of the invention is to develop a method of analysis of flavonoids in Choleretic collection No. 3".
The technical result of the proposed method is an improvement of a method of quantitative analysis "Cholagogue collection No. 3", by identification of flavonoids as bioactive compounds, determine the main pharmacological properties of the collection.
W is the loss in weight of the raw material during drying, in percent.
In the analysis measure the optical density of the complex of flavonoids with aluminum chloride of the analyzed solution in the background of the initial solution. In this case, the observed bathochromic shift of the long wavelength bands of flavonoids, which is found in the UV spectrum as the absorption maximum at a wavelength of 406 nm. This is confirmed in terms of differential spectrophotometry: absorption maximum at 406 nm (Fig. 1). The study of UV spectra of the state standard sample of cynaroside showed (Fig. 2) that the solution of this standard in the presence of aluminum chloride is close to the maximum absorption (400 nm). Consequently, cynaroside can be used in the method of analysis as a standard sample.
We studied the spectral characteristics of water-alcohol extraction from raw plant components "Geelong�nny collection No. 3" (Fig. 3), to determine the content of total flavonoids in each component of the collection (Fig. 4).
Based on the spectral characteristics of components of data collection and model compound (Fig. 1, 3), to determine the optimal extraction conditions "Cholagogue collection No. 3" has developed a method for determination of total flavonoids in the Choleretic collection No. 3 by the method of differential spectrophotometry in terms of cynaroside.
An analytical sample of the raw material is ground to a particle size passing through a sieve with holes of diameter 1 mm. to About 1 g of the crushed raw material (a precisely weighed) was placed in a flask with a ground joint with a capacity of 50 ml, add 30 ml of 70% ethanol. The flask is closed with a stopper and weighed on terimah scales with an accuracy of ±0.01 g. the Flask is attached to the reverse refrigerator and heated in a boiling water bath (moderate boiling) for 90 min, Then the flask close to the same tube, weighed again and fill the missing extractant to the initial weight. The extract was filtered through filter paper and cooled for 30 min. the Test solution was prepared as follows: 1 ml of extract obtained is placed in a volumetric flask with a capacity of 25 ml, add 1 ml of a 3% alcohol solution of aluminum chloride and bring the volume of solution up to the mark with 95% ethyl alcohol (test solution a). In CA�ETS of the reference solution using a solution prepared under the same conditions, but without addition of aluminum chloride solution (reference solution A). Optical density measurements carried out on the spectrophotometer at a wavelength of 400 nm. In parallel, measure the optical density of the solution of the state standard sample of cynaroside at a wavelength of 400 nm, prepared by analogy with the test solution (see note).
Note. Preparation of solution of cynaroside-standard sample. About 0.02 (a precisely weighed) of cynaroside placed in a volumetric flask with a capacity of 25 ml, was dissolved in 15-20 ml of 70% ethyl alcohol by heating on a water bath. After cooling the contents of the flask to room temperature the volume was adjusted solution of 70% ethanol to the mark (solution A). 1 ml of a solution of cynaroside placed in a volumetric flask of 25 ml, add 1 ml of a 3% alcohol solution of aluminum chloride and the volume was adjusted solution of 95% ethanol to the mark and mix (test solution B). As of the reference solution use a solution prepared as follows: 10 ml of solution A of cynaroside placed in a volumetric flask of 25 ml and the volume was adjusted solution up to the mark with 95% ethanol (reference solution B).
The proposed method is illustrated by the following examples.
An analytical sample of the raw material (collection "Hitogata No. 3" ("Cholagogue collection No. 3"), OAO "Krasnogorskleksredstva", series 60912) is ground to a particle size passing through a sieve with holes of diameter 1 mm. to About 1 g of the crushed raw material (a precisely weighed) was placed in a flask with a ground joint with a capacity of 50 ml, add 30 ml of 70% ethanol. The flask is closed with a stopper and weighed on terimah scales with an accuracy of ±0.01 g. the Flask is attached to the reverse refrigerator and heated in a boiling water bath (moderate boiling) for 90 min, Then the flask close to the same tube, weighed again and fill the missing extractant to the initial weight. The extract was filtered through filter paper and cooled for 30 min. the Test solution was prepared as follows: 1 ml of extract obtained is placed in a volumetric flask with a capacity of 25 ml, add 1 ml of a 3% alcohol solution of aluminum chloride and bring the volume of solution up to the mark with 95% ethyl alcohol (test solution a). As of the reference solution use the solution prepared under the same conditions, but without addition of aluminum chloride (solution CPA�tion A). Optical density measurements carried out on the spectrophotometer at a wavelength of 400 nm. In parallel, measure the optical density of the solution of the standard sample of cynaroside at a wavelength of 400 nm, prepared by analogy with the test solution (see note).
The optical density D=0,413569, the raw material mass m=0,9973.
The content of total flavonoids = 1,05%.
All results were statistically processed. A single error quantification is ±3,12%.
Open 10 filter bags (collection "Hitogata No. 3" ("Cholagogue collection No. 3"), OAO "Krasnogorskleksredstva", filter bags, series bearings 30213). About 1 g of the crushed material (a precisely weighed) was placed in a flask with a ground joint with a capacity of 50 ml. Then the process is carried out in accordance with example 1.
The optical density D=0,597248, the raw material mass m=1,0016.
The content of total flavonoids = 1,16%.
All results were statistically processed. A single error quantification ±or 3.28%.
An analytical sample model compound, the structure represented in Fig. 5, crushed to a particle size passing through a sieve with holes of diameter 1 mm. to About 1 g of the crushed raw material (a precisely weighed) was placed in a flask with a ground joint with a capacity of 50 ml. Then the process is carried out in accordance with example 1.
The optical density D=0,526487, the raw material mass m=0,9963.
The content of total flavonoids = 1,03%.
All results were statistically processed. A single error quantification is ±3,19%.
1. The definition is the content of water-soluble substances - flavonoids, instead of essential oils, the components of which are virtually insoluble in water.
2. This method allows to evaluate the content of total flavonoids as bioactive components have a major therapeutic effect choleretic effect.
3. The developed method is specific, because it allows to eliminate the contribution of other compounds (taking into account the ability of flavonoids to form complexes with aluminum chloride).
1. VFS 42-2558-95. Cholagogue collection No. 3 / state Pharmacopoeia Committee. Introduction. 09.07.1996. - M., 1996. - 12 p.
2. The state register of medicines. Official edition as of April 1, 2009: in 2 t. T. 1. - M.: Publishing house "Medical Council�, 2009. - 1359 S.
3. State Pharmacopoeia of the USSR. - Eleventh edition. - Vol. 1. - M.: Medicine, 1987. - 336.
4. State Pharmacopoeia of the USSR. - Eleventh edition. - Vol. 2. - M.: Medicine, 1990. - 400 p.
5. Kurkin V. A. Fundamentals of herbal medicine: a textbook for students of pharmaceutical universities. - Samara: LLC "Etching", GOU VPO "Samara state medical University of Roszdrav", 2009. - 963 S.
7. Kurkin V. A. Prospects for the creation and implementation of import-substituting medicinal herbal remedies / V. A. Kurkin, E. V. Avdeeva, V. B. Braslavsky, O. Pravdivtseva, A. V. Kurkina et al. / / XVIII Russian national Congress "Man and medicine: abstracts. - Moscow, 2011. - P. 507.
8. Kurkin V. A. Current aspects of standardization of medicinal plants containing flavonoids / V. A. Kurkin // materials of the Interregional scientific conference with international participation, dedicated to the 70th anniversary of pharmaceutical faculty, Siberian state medical University. - Tomsk, 2011. - Pp. 86-90.
9. Kurkin V. A. New approaches to the standardization of medicinal plants containing flavonoids. 1. Common tansy / V. A. Kurkin // 65-th international conference on pharmacy and pharmacology "Development�, research and marketing of new pharmaceutical products": collection of scientific works. - Pyatigorsk, 2011. - Vol. 66. - Pp. 134-137.
10. Kurkin V. A. Flavonoids as a source of medicinal preparations on the basis of raw materials of pharmacopoeial plants / V. A. Kurkin // 3rd international Congress of herbalists and herbalists "Modern problems of herbal medicine and Travnikova: proceedings of scientific works. - M., 2013. - P. 111-114.
SUBSTANCE: method of Novocain extraction from water solutions includes the preparation of an aqueous salt solution of Novocain by its dissolution in a saturated solution of a salting-out agent, extraction and analysis of an equilibrium water phase, with the application as an extragent of a solvotrophic reagent solution in chloroform with the concentration of 10 wt %, for which purpose the aqueous salt solution of Novocain with pH 8.0±0.5 is prepared due to the application of a saturated ammonium sulphate solution as the salting-out agent and addition of an ammonium buffer solution, Novocain is extracted for 5-7 minutes with the solution of the solvotrophic reagent in chloroform with the ratio of volumes of the aqueous salt solution of Novocain and extragent of 5:1, then the aqueous saline phase is separated from the organic one and analysed by a method of UV-spectrophotometry at a wavelength of 291 nm, the concentration of Novocain in the water solution is found by means of a calibrating graph; the coefficient of distribution (D) and the degree of extraction (R, %) of Novocain is are calculated by formulae.
EFFECT: method for the extraction of Novocain from the water solution, which is characterised by expressiveness, makes it possible to realise the practically complete single extraction of Novocain from the aqueous salt solution and can be applied in the analysis of Novocain-containing water solutions.
SUBSTANCE: invention relates to analytical chemistry and can be used in a system for monitoring content of sodium thiosulphate in solutions. The method of determining sodium thiosulphate in solutions is characterised by adding an analysed sample into a reaction vessel containing a corresponding amount of photogenerated iodine, obtained by blowing with air for 1-2 minutes and irradiating the reaction mixture with a stabilised light source, the mixture consisting of 0.5 M potassium iodide solution, an acetate buffer solution with pH 5.6 and a sodium eosinate sensitising agent, by detecting change in current in a cell and upon achieving constant current, re-blowing the reaction mixture with air for 2-3 minutes and re-irradiating with the stabilised light source until achieving the initial amount of iodine in the vessel, measuring the iodine generation time spent on achieving the decrease thereof, determining the amount of sodium thiosulphate from a calibration curve from the change in current and generation time.
EFFECT: invention provides a simple method of determining sodium thiosulphate in solutions and avoids use of expensive equipment.
SUBSTANCE: invention describes a method for lipoic acid measurement in biologically active additives by cathode voltammetry involving transferring a substance from a sample into a solution and taking voltammetric measurement; the cathode voltammetry is performed on a mercury-film electrode at potential -0.373 V of a relatively saturated silver-chloride electrode with borate buffer solution pH 9.18 at continuously current potential trace at 0.06 V/s with the determined lipoic acid content range from 4.5·106 to 1.1·10-3 mole/l.
EFFECT: improving sensitivity and expressivity of the method for measuring lipoic acid in tabletted BAAs by cathode voltammetry.
SUBSTANCE: in agar plate which is in a Petri dish and inoculated with one of the fungal species of the genus Fusarium, circular holes are cut with the diameter of 8 mm, 0.05 ml of the working solution of the test preparation is applied into them, and placed in a thermostat at a temperature of 24.5-25.0°C, after 2-5 days depending on the type of fungus the diameter of the zone of growth inhibition of fungal mycelium is measured and the activity of the preparations is calculated according to the formula , and it is considered that when A=1 the fungicide is ineffective - the zone of growth inhibition is not formed (D=d), when A=2-3 the preparation activity is low, when A=4 it is average, and when A≥5 it is high.
EFFECT: invention provides accuracy of determining the activity of seed disinfectants and fungicides used in agricultural production.
SUBSTANCE: invention represents a method for preclinical study of cardiotropic antiarrhythmic drugs, involving determining the bioelectric parameters in isolated multicellular perfused preparations and measuring an action potential duration, differing by the fact that the isolated multicellular perfused preparations are presented by rat's pulmonary vein myocardium; the parameters are measured in three operation modes of the multicellular preparations; a resting potential is additionally measured; varying APD 90%, related APD 50%/APD 90%, a spontaneous shear velocity of the resting potential, the most positive membrane potential in the resting preparation, a spontaneous activity train repetition rate, spontaneous action potential train repetition and variability frequency, post-depolarisation number and intensity, as well as a shear membrane potential corresponding to the beginning of train activity are used to evaluate the signs of antiarrhythmic and arrhythmogenic action.
EFFECT: more reliable prediction of the antiarrhythmic action of the potential pharmacological agents and reduction of experimental phase time.
SUBSTANCE: invention relates to a method and a system for analysing or inspecting people or other objects for presence of foreign or native materials. The method employs parallel-mode spectroscopy, which comprises generating a probing signal simultaneously containing electromagnetic radiation with a wide bandwidth in the range of 10 GHz to 25 THz. Said range enables simultaneous detection of a plurality of signals at a plurality of frequencies. Each signal has certain amplitude, which collectively provides a unique spectral signature of the material. Processing of the obtained data comprises presenting said data in matrix form. Correlation method is used to compare the obtained matrix data with a reference library.
SUBSTANCE: method consists in measurement of coefficient of absorption of infrared radiation the analysed mix of gases containing 14CO2 or 14CO passed through single-pass or multipass to a ditch, thus measurement of coefficient of absorption of radiation is taken in the range of lengths of waves from 1.94 to 2.18 microns, or from 2.50 to 2.80 microns, or from 4.00 to 4.50 microns, or from 14.00 to 17.00 microns for molecules 14CO2, and in the range of lengths of waves from 2.30 to 2.50 microns or from 4.50 to 5.10 microns for molecules 14CO. The selection of the named ranges, the spectral accuracy and ditche type is caused by presence of other main gas components, formed during processing of spent nuclear fuel, and also by the concentration of the molecules 14CO2 or 14CO and the value of the common pressure in the ditch.
SUBSTANCE: invention relates to microscopy of individual biological organisms in a liquid sample. Images on which individual biological organisms can be identified are combined to provide sets of optical sections of the biological organisms and the sets of optical sections are analysed to determine the value of said at least one parameter describing microbial activity of said individual biological organism in each sample container.
EFFECT: shorter analysis time, high reliability and cost-effectiveness of the result.
SUBSTANCE: infrared spectrometer is used to determine presence of radioactive gases and aerosols in the air by determining high content of ozone in the air formed from oxygen under the effect of ionising radiation of radionuclides.
EFFECT: invention reduces radiation dose by taking protective measures which enable to prevent inhalation of radionuclides before the arrival of a radioactive cloud in a region occupied by humans.
SUBSTANCE: one performs tobacco sample irradiating with a ray within the near-IR range and measures transmission and absorbance spectrum or diffuse reflection spectrum. The measured transmission and absorbance spectrum or diffuse reflection spectrum is used to calculate the estimated value of filling capacity according to a preliminarily plotted calibration curve.
SUBSTANCE: proposed method comprises consecutive feed of lumps in control zone for irradiation and registration of wanted signal. Said lumps are fed in said zone in fixe path with lump sides' fixation relative to wanted signal registration radiation directions with setting of unlimited control zones passages with variable irradiation and registration parameters. Proposed method is realized with the help of the device including the case, lumps feeder, irradiator and radiation receiver as well as control device. Irradiator and receiver incorporate their drives. Lumps feeder is composed of multisection trick displacing on guide shaft and provided with specimen fastener and driven by step motor with the help of drive belt. Microprocessor-based control device controls the speed of passing by of control zone, operation of irradiator, receiver and their drives as well as a series of logically related experiments with specimens runs through control zone.
SUBSTANCE: invention relates to a method of making a sensor for obtaining giant Raman scattering spectra, which is a glass capillary whose inner wall is coated with silver nanoparticles. The silver nanoparticles are obtained and attached to the glass surface via reduction of silver ions with alkylamines. The glass capillaries are washed with a detergent solution for optical devices, distilled water while mixing with ultrasound, absolute ethanol and dried in air, placed in a teflon vessel with a reaction mixture of 1 mmol/l AgNO3 and 1 mmol/l alkylamine in ethanol; the reaction mixture is heated at 45-50°C for 40 min with intense agitation along the axis of the capillaries. After the reduction reaction, the capillaries are washed with ethanol and cleaned on the outside.
EFFECT: invention enables to obtain a high-resolution giant Raman scattering sensor.
SUBSTANCE: invention is related to research of composition and properties of multicomponent hydrocarbon systems in process of oil and gas condensate deposits, and namely to photometric methods for determination of diethylene glycol concentration in saturated (upon moisture absorption from gas) diethylene glycol (sDEG) and recovered diethylene glycol (rDEG). DEG concentration in field diethylene glycol solutions is measured by IR-spectrometric method that includes determination of their optical density and determination of DEG content against pre-plotted calibration dependence of optical density on DEG concentration in the solvent such as diethylene glycol of SOP brand (99.9%), which is also used as a blank sample during calibration and measurements. At that, before measurement of optical density of calibration solvents and the tested sample, preliminary scanning of their spectra is done and wave length corresponding to the maximum signal in the measured spectrum is fixed, while measurement of optical density for the blank, calibration and tested samples are made at wave lengths corresponding to the maximum value of the signal for each sample.
EFFECT: invention allows fast determination of DEG content with high accuracy and without sample treatment.
SUBSTANCE: invention relates to nano-, microelectronics and analytical instrument making industry and can be used for development of technologies and for production of products of micro- and nanoelectronics, as well as for production of pure materials and for diagnostics and control of liquid process media. A method for determining atomic composition of active impurities in liquid media consists in preparation of an analysed object and its arrangement in vacuum. Then, irradiation of surface with a beam of charged particles and recording of secondary particles, as per which composition of surface atoms is determined, is performed. Preparation of the analysed object is performed by preparation of surface of a semiconductor plate by chemical etching, treatment in a peroxide alkali solution and by washing in deionised water. A drop of analysed liquid with the size of at least one micron is applied to the prepared surface of a clean semiconductor plate, then, it is removed, and for the purpose of analysis, the removed drop trace is irradiated with a beam of charged particles.
EFFECT: improvement of analysis rapidness, as well as improvement of a detection limit, and namely at least by 10-100 times.
FIELD: investigating or analyzing of materials.
SUBSTANCE: method comprises irradiating a thin layer of a liquid sample to be investigated by laser pulse which is adsorbed by the layer or substrate. The properties of the liquid can be judged by characteristic features of the development and relaxation of the thermocapillary convection which is observed with the use of low-power laser beam in the visible range.
EFFECT: enhanced reliability of investigating.

References: V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V. 
 V.