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Timestamp: 2019-04-21 18:46:06+00:00

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Multiphoton microscopy (also known as non-linear or two-photon microscopy) is an alternative to laser scanning (single photon) or deconvolution microscopy that provides distinct and clear advantages for three-dimensional imaging. Specifically, multiphoton excitation is superior for imaging living cells that reside within intact tissues such as brain slices, embryos, whole organs, and even entire animals. The technique provides optical sectioning with reduced absorption of excitation light in specimen regions removed from the objective focal plane, thus minimizing photobleaching and phototoxicity. The longer excitation wavelengths also enable increased depth penetration over confocal microscopy and are therefore more useful to deep imaging.
Diaspro, A., Chirico, G. and Collini, M. Two-photon fluorescence excitation and related techniques in biological microscopy. Quarterly Reviews of Biophysics 38: 97-166(2005).
Piston, D. W. Imaging living cells and tissues by two-photon excitation microscopy. Trends in Cell Biology 9: 66-69 (1999).
Dunn, K. W. and Young, P. A. Principles of multiphoton microscopy. Experimental Nephrology 103: e33-e40 (2006).
Zipfel, W. R., Williams, R. M. and Webb, W. W. Nonlinear magic: multiphoton microscopy in the biosciences. Nature Biotechnology 21: 1369-1377 (2003).
Benninger, R. K. P., Hao, M. and Piston, D. W. Multi-photon excitation imaging of dynamic processes in living cells and tissues. Reviews of physiology, Biochemistry and Pharmacology 160: 71-92 (2008).
Helmchen, F. and Denk, W. Deep tissue two-photon microscopy. Nature Methods 2: 932-940 (2005).
Gratton, E., Barry, N. P., Beretta, S. and Celli, A. Multiphoton fluorescence microscopy.Methods 25: 103-110 (2001).
Konig, K. Multiphoton microscopy in life sciences. Journal of Microscopy 200: 83-104 (2000).
So, P. T. C., Dong, C. Y., Masters, B. R. and Berland, K. M. Two-photon excitation fluorescence microscopy. Annual Review of Biomedical Engineering 2: 399-429 (2000).
Periasamy, A. and Diaspro, A. Multiphoton Microscopy. Journal of Biomedical Optics 8: 327-328 (2003).
Helmchen, F. and Denk, W. New developments in multiphoton microscopy . Current Opinion in Neurobiology 12: 593-601 (2002).
Piston, D. W. The coming of age of two-photon excitation imaging for intravital microscopy. Advanced Drug Delivery Reviews 58: 770-772 (2006).
Centonze, V. E. and White, J. G. Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophysical Journal 75: 2015-2024 (1998).
Denk, W., Strickler, J. H. and Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248: 73-76 (1990).
Denk, W. and Svoboda, K. Photon upmanship: why multiphoton imaging is more than a gimmick. Neuron 18: 351-357 (1997).
Cox, G. and Sheppard, C. J. R. Practical limits of resolution in confocal and non-linear microscopy Microscopy Research and Technique 63: 18-22 (2004).
Bestvater, F., Spiess, E., Stobrawa, G., Hacker, M., Feurer, T., Porwol, T., Berchner-Pfannschmidt, U., Wotzlaw, C. and Acker, H. Two-photon fluorescence absorption and emission spectra of dyes relevant for cell imaging. Journal of Microscopy 208: 108-115 (2002).
Blab, G. A., Lommerse, P. H. M., Cognet, L., Harms, G. S. and Schmidt, T. Two-photon excitation action cross-sections of the autofluorescent proteins. Chemical Physics Letters350: 71-77 (2001).
Albota, M. A., Beljonne, D., Bredas, J. L, Ehrlich, J. E., Fu, J. Y., Heikal, A. A., Hess, S. E., Kogej, T., Levin, M. D., Marder, S. R., McCord-Maughon, D., Perry, J. W., Rockel, H., Rumi, M., Subramaniam, G., Webb, W. W., Wu, X. L. and Xu, C. Design of organic molecules with large two-photon absorption cross sections. Science 281: 1653-1656 (1998).
Albota, M. A., Xu, C. and Webb, W. W. Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm. Applied Optics 37: 7352-7356 (1998).
Andrews, D. L. A simple statistical treatment of multiphoton absorption. American Journal of Physics 53: 1001-1002 (1984).
Ashkin, A. and Dziedzic, J. M. Optical trapping and manipulation of viruses and bacteria.Science 235: 1517-1520 (1987).
Axe, Jr., J. D. Two-photon processes in complex atoms. Physical Review 136: A42-A45 (1964).
Barad, Y., Eisenberg, H., Horowitz, M. and Silberberg, Y. Nonlinear scanning laser microscopy by third harmonic generation . Applied Physics Letters 70: 922-924 (1997).
Beaurepaire, E., Oheim, M. and Mertz, J. Ultra-deep two-photon fluorescence excitation in turbid media. Optics Communications 188: 25-29 (2000).
Benham, G. S. and Schwartz, S. Suitable microscope objectives for multiphoton digital imaging. Proceedings of SPIE 4620: 36-47 (2002).
Bennett, B. D., Jetton, T. L., Ying, G., Magnuson, M. A. and Piston, D. W. Quantitative subcellular imaging of glucose metabolism within intact pancreatic islets. Journal of Biological Chemistry 271: 3647-3651 (1996).
Benninger, R. K. P., Onfelt, B., Neil, M. A. A., Davis, D. M. and French, P. M. W. Fluorescence imaging of two-photon linear dichroism: cholesterol depletion disrupts molecular orientation in cell membranes. Biophysical Journal 88: 609-622 (2005).
Berland, K. M., So, P. T. C., Chen, Y., Mantulin, W. W. and Gratton, E. Scanning two-photon fluctuation correlation spectroscopy: particle counting measurements for detection of molecular aggregation. Biophysical Journal 71: 410-420 (1996).
Berland, K. M., So, P. T. C. and Gratton, E. Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment Biophysical Journal 68: 694-701 (1995).
Berns, M. W. A possible two-photon effect in vitro using a focused laser beam. Biophysical Journal 16: 973-977 (1976).
Bewersdorf, J. and Hell, S. W. Picosecond pulsed two-photon imaging with repetition rates of 200 and 400 MHz. Journal of Microscopy 191: 28-38 (1998).
Bewersdorf, J., Pick, R. and Hell, S. W. Multifocal multiphoton microscopy. Optics Letters23: 655-657 (1998).
Birge, R. R. Two-photon spectroscopy of protein-bound chromophores. Accounts of Chemical Research 19: 138-146 (1986).
Birge, R. R. and Pierce, B. M. A theoretical analysis of the two-photon properties of linear polyenes and the visual chromophores. Journal of Chemical Physics 70: 165-178 (1979).
Blinova, K., Combs, C., Kellman, P. and Balaban, R. S. Fluctuation analysis of mitochondrial NADH fluorescence signals in confocal and two-photon microscopy images of living cardiac myocytes. Journal of Microscopy 213: 70-75 (2003).
Booth, M. J. and Hell, S. W. Continuous wave excitation two-photon fluorescence microscopy exemplified with the 647-nm ArKr laser line. Journal of Microsopy 190: 298-304 (1998).
Booth, M. J., Neil, M. A. A. and Wilson, T. New modal wave-front sensor: application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy. Journal of the Optical Society of America 19: 2112-2120 (2002).
Brakenhoff, G. J., Muller, M. and Ghauharali, R. I. Analysis of efficiency of two-photon versus single-photon absorption for fluorescence generation in biological objects. Journal of Microscopy 183: 140-144 (1996).
Brown, E. B., Shear, J. B., Adams, S. R., Tsien, R. Y. and Webb, W. W. Photolysis of caged calcium in femtoliter volumes using two-photon excitation. Biophysical Journal 76: 489-499 (1999).
Buist, A. H., Muller, M., Squier, J. and Brakenhoff, G. J. Real time two-photon absorption microscopy using multi point excitation. Journal of Microscopy 192: 217-226 (1998).
Cahalan, M. D., Parker, I., Wei, S. H. and Miller, M. J. Two-photon tissue imaging: seeing the immune system in a fresh light. Nature Reviews 2: 872-880 (2002).
Callis, P. R. Two-photon-induced fluorescence. Annual Review of Physical Chemistry 48:271-297 (1997).
Campagnola, P. J., Wei, M. D., Lewis, A. and Loew, L. M. High-resolution nonlinear optical imaging of live cells by second harmonic generation. Biophysical Journal 77: 3341-3349 (1999).
Cannone, F., Chirico, G. and Diaspro, A. Two-photon interactions at single fluorescent molecule level. Journal of Biomedical Optics 8: 391-395 (2003).
Chen, Y., Muller, J. D., So, P. T. C. and Gratton, E. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophysical Journal 77: 553-567 (1999).
Chirico, G., Bettati, S. Mozzarelli, A., Chen, Y., Muller, J. D. and Gratton, E. Molecular heterogeneity of O-Acetylserine sulfhydrylase by two-photon excited fluorescence fluctuation spectroscopy. Biophysical Journal 80: 1973-1985 (2001).
Chirico, G., Cannone, F., Baldini, G. and Diaspro, A. Two-photon thermal bleaching of single fluorescent molecules. Biophysical Journal 84: 588-598 (2003).
Chirico, G., Cannone, F., Beretta, S., Baldini, G. and Diaspro, A. Single molecule studies by means of the two-photon fluorescence distribution. Microscopy Research and Technique55: 359-364 (2001).
Chirico, G., Cannone, F., Diaspro, A., Bologna, S., Pellegrini, V., Nifosi, R. and Beltram, F.Multiphoton switching dynamics of single green fluorescent proteins. Physical Review 70:030901-4 (2004).
Chirico, G., Olivini, F. and Beretta, S. Fluorescence excitation volume in two-photon microscopy by autocorrelation spectroscopy and photon counting histogram. Applied Spectroscopy 54: 1084-1090 (2000).
Deguil, N., Mottay, E., Salin, F., Legros, P. and Choquet, D. Novel diode-pumped infrared tunable laser system for multi-photon microscopy. Microscopy Research and Technique63: 23-26 (2004).
Denk, W., Delaney, K. R., Gelperin, A., Kleinfeld, D., Strowbridge, B. W., Tank, D.W. and Yuste, R., Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy. Journal of Neuroscience Methods 54: 151-162 (1994).
Despa, S., Kockskamper, J., Blatter, L. A. and Bers, D. M. Na/K pump-induced [Na]I Gradients in rat ventricular myocytes measured with two-photon microscopy. Biophysical Journal87: 1360-1368 (2004).
Diaspro, A. Introduction to two-photon microscopy. Microscopy Research and Technique47: 163-164 (1999).
Diaspro, A. Rapid dissemination of two-photon excitation microscopy prompts new applications. Microscopy Research and Technique 63: 1-2 (2004).
Diaspro, A., Chirico, G., Federici, F., Cannone, F., Beretta, S. and Robello, M. Two-photon microscopy and spectroscopy based on a compact confocal scanning head. Journal of Biomedical Optics 6: 300-310 (2001).
Diaspro, A., Corosu, M., Ramoino, P. and Robello, M. Adapting a compact confocal microscope system to a two-photon excitation fluorescence imaging architecture.Microscopy Research and Technique 47: 196-205 (1999).
Diaspro, A., Federici, F., Viappiani, C., Krol, S., Pisciotta, M., Chirico, G., Cannone, F. and Gliozzi, A. Two-photon photolysis of 2-nitrobenzaldehyde monitored by fluorescent-labeled nanocapsules. Journal of Physical Chemistry 107: 11008-11012 (2003).
Diaspro, A. and Robello, M. Two-photon excitation of fluorescence for three-dimensional optical imaging of biological structures. Journal of Photochemistry and Photobiology B:Biology 55: 1-8 (2000).
Dittrich, P. S. and Schwille, P. Photobleaching and stabilization of fluorophores used for single-molecule analysis with one- and two-photon excitation. Applied Physics B 73: 829-837 (2001).
Dittrich, P. S. and Schwille, P. Spatial two-photon cross-correlation spectroscopy for controlling molecular transport in microfluidic structures. Analytical Chemistry 74: 4472-4479 (2002).
Drummond, D. R., Carter, N. and Cross, R. A. Multiphoton versus confocal high resolution z-sectioning of enhanced green fluorescent microtubules: increased multiphoton photobleaching within the focal plane can be compensated using a Pockels cell and dual widefield detectors. Journal of Microscopy 206: 161-169 (2002).
Eggeling, C., Volkmer, A. and Seidel., C. A. M. Molecular Photobleaching Kinetics of Rhodamine 6G by One- and Two-Photon Induced Confocal Fluorescence Microscopy.Chemphyschem 6: 791-804 (2005).
Eng, J., Lynch, R. M. and Balaban, R. S. Nicotinamide adenine dinucleotide fluorescence spectroscopy and imaging of isolated cardiac myocytes. Biophysical Journal 55: 621-630 (1989).
Esposito, A., Federici, F., Usai, C., Cannone, F., Chirico, G., Collini, M. and Diaspro, A. Notes on Theory and Experimental Conditions Behind Two-Photon Excitation Microscopy.Microscopy Research and Technique 63: 12-17 (2004).
Fan, G. Y., Fujisaki, H., Miyawaki, A., Tsay, R. K., Tsien, R. Y. and Ellisman, M. H. Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons. Biophysical Journal 76: 2412-2420 (1999).
Feijo, J. A. and Moreno, N. Imaging plant cells by two-photon excitation. Protoplasma 223:1-32 (2004).
Freeman, R. G., Gilliland, D. L. and Lytle, F. E. Second harmonic detection of sinusoidally modulated two-photon excited fluorescence. Analytical Chemistry 62: 2216-2219 (1990).
French, T., So, P. T. C., Weaver, D. J., Coelho-Sampaio, T., Gratton, E., Voss, E. W. and Carrero, J. Two-photon fluorescence lifetime imaging microscopy of macrophage-mediated antigen processing. Journal of Microscopy 185: 339-353 (1997).
Friedrich, D. M. and McClain, W. M. Two-photon molecular electronic spectroscopy. Annual Review of Physical Chemistry 31: 559-577 (1980).
Gauderon, R., Lukins, P. B. and Sheppard, C. J. R. Effect of a confocal pinhole in two-photon microscopy. Microscopy Research and Technique 47: 210-214 (1999).
Gaus, K., Gratton, E., Kable, E. P. W., Jones, A. S., Gellssen, I., Kritharides, L. and Jessup, W.Visualizing lipid structure and raft domains in living cells with two-photon microscopy.Proceedings of the National Academy of Sciences (USA) 100: 15554-15559 (2003).
Girkin, J. M. Optical physics enables advances in multiphoton imaging. Journal of Physics36: R250-R258 (2003).
Gratton, E., Breusegem, S., Sutin, J., Ruan, Q. and Barry, N. Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods. Journal of Biomedical Optics 8: 381-390 (2003).
Gu, M. Resolution in three-photon fluorescence scanning microscopy. Optics Letters 21:988-990 (1996).
Guiot, E., Georges, P., Brun, A., Fontaine, M. P., Bellon-Fontaine, M. N. and Briandet, R.Heterogeneity of diffusion inside microbial biofilms determined by fluorescence correlation spectroscopy under two-photon excitation. Photochemistry and Photobiology75: 570-578 (2002).
Hanninen, P., Soukka, J. and Soini, J. T. Two-photon excitation fluorescence bioassays.Annals of the New York Academy of Sciences 1130: 320-326 (2008).
Heikal, A. A., Hess, S. T. and Webb, W. W. Multiphoton molecular spectroscopy and excited-state dynamics of enhanced green fluorescent protein (EGFP): acid-base specificity.Chemical Physics 274: 37-55 (2001).
Heinze, K. G., Koletermann, A. and Schwille, P. Simultaneous two-photon excitation of distinct labels for dual-color fluorescence cross correlation analysis. Proceedings of the National Academy of Sciences (USA) 97: 10377-10382 (2000).
Hell, S. W. and Andresen, V. Space-multiplexed multifocal nonlinear microscopy. Journal of Microscopy 202: 457-463 (2001).
Hopt, A. and Neher, E. Highly nonlinear photodamage in two-photon fluorescence microscopy. Biophysical Journal 80: 2029-2036 (2001).
Huang, S., Heikal, A. A. and Webb, W. W. Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein. Biophysical Journal 82: 2811-2825 (2002).
Imanishi, Y., Batten, M.L., Piston, D.W., Baehr, W. and Palxzewski, K. Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye. Journal of Cell Biology 164:373-383 (2004).
Imanishi, Y., Lodowski, K. H. and Koutalos, Y. Two-photon microscopy: shedding light on the chemistry of vision. Biochemistry 46: 9674-9684 (2007).
Jenei, A., Kirsch, A. K., Subramaniam, V., Arndt-Jovin, D. J. and Jovin, T. M. Picosecond multiphoton scanning near-field optical microscopy. Biophysical Journal 76: 1092-1100 (1999).
Jung, J.C. and Schnitzer, M. J. Multiphoton endoscopy. Optics Letters 28: 902-904 (2003).
Kaiser, W. and Garrett, C. G. B. Two-photon excitation in CaF2:Eu2+. Physical Review Letters 7: 229-232 (1961).
Kennedy, S. M. and Lytle, F. E. p-Bis (o-methylstyryl) benzene as a power-squared sensor for two-photon absorption measurements between 537 and 694 nm. Analytical Chemistry58: 2643-2647 (1986).
Kerr, J. N. D. and Denk, W. Imaging in vivo: watching the brain in action. Nature Reviews 9:195-205(2008).
Kim, S. A., Heinze, K. G., Bacia, K., Waxham, M. N. and Schwille, P. Two-photon cross-correlation analysis of intracellular reactions with variable stoichiometry. Biophysical Journal 88: (2005).
Kirkpatrick, S. M., Naik, R. R. and Stone, M. O. Nonlinear saturation and determination of the two-photon absorption cross section of green fluorescent protein. Journal of Physical Chemistry 105: 2867-2873 (2001).
Kirsch, A. K., Subramaniam, V., Striker, G., Schnetter, C., Arndt-Jovin, D. J. and Jovin, T. M.Continuous wave two-photon scanning near-field optical microscopy. Biophysical Journal75: 1513-1521 (1998).
Koester, H. J., Baur, D., Uhl, R. and Hell, S. W. Calcium ion fluorescence imaging with pico-and femtosecond two-photon excitation: signal and photodamage. Biophysical Journal77: 2226-2236 (1999).
Konig, K., Gohlert, A., Liehr, T., Loncarevic, I. F. and Riemann, I. Two-photon multicolor FISH: a versatile technique to detect specific sequences within single DNA molecules in cells and tissues. Single Molecules 1: 41-51 (2000).
Lakowicz, J. R., Gryczynski, I., Malak, H., Schrader, M., Engelhardt, P., Kano, H. and Hell, S. W.Time-resolved fluorescence spectroscopy and imaging of DNA labeled with DAPI and Hoechst 33342 using three-photon excitation. Biophysical Journal 72: 567-578 (1997).
Larson, D. R., Zipfel, W. R., Williams, R. M., Clark, S. W., Bruchez, M. P., Wise, F. W. and Webb, W. W. Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science300: 1434-1436 (2003).
Lindegger, N. and Niggli, E. Paradoxical SR Ca2+release in guinea-pig cardiac myocytes after B-adrenergic stimulation revealed by two-photon photolysis of caged Ca 2+. The journal of physiology 565: 801-813 (2005).
Lipp, P. and Niggli, E. Fundamental calcium release events revealed by two-photon excitation photolysis of caged calcium in guinea-pig cardiac myocytes. Journal of Physiology 508: 801-809 (1998).
Lucitti, J. L. and Dickinson, M. E. Moving Toward the light: using new technology to answer old questions. Pediatric Research 60: 1-5 (2006).
Mainen, Z. F., Maletic-Savatic, M., Shi, S. H., Hayashi, Y., Malinow, R. and Svoboda, K. Two-photon imaging in living brain slices. Methods 18: 231-239 (1999).
Maiti, S., Shear, J. B., Williams, R. M., Zipfel, W. R. and Webb, W. W. Measuring serotonin distribution in live cells with three-photon excitation. Science 275: 530-532 (1997).
Majewska, A., Yiu, G. and Yuste, R. A custom-made two-photon microscope and deconvolution system. European Journal of Physiology 441: 398-408 (2000).
Majoul, I., Straub, M., Duden, R., Hell, S. W. and Soling, H. D. Fluorescence resonance energy transfer analysis of protein-protein interactions in single living cells by multifocal multiphoton microscopy. Reviews in Molecular Biotechnology 82: 267-277 (2002).
Masters, B. R. and So, P. T. C. Antecedents of two-photon excitation laser scanning microscopy. Microscopy Research and Technique 63: 3-11 (2004).
Masters, B. R., So, P. T. C. and Gratton, E. Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin. Biophysical Journal 72: 2405-2412 (1997).
McClain, W. M. Excited state symmetry assignment through polarized two-photon absorption studies of fluids. Journal of Chemical Physics 55: 2789-2796 (1971).
McConnell, G., Girkin, J. M., Gurney, A. M., Langford, N. and Ferguson, A. I. All-solid-state sub-picosecond optical parametric oscillator laser system for multi-photon fluorescence imaging. IEEE 273 (2003).
Mertz, J. Molecular photodynamics involved in multi-photon excitation fluorescence microscopy. The European Physical Journal D 3: 53-66 (1998).
Milburn, T., Matsubara, N., Billington, A. P., Udgaonkar, J. B., Walker, J. W., Carpenter, B. K., Webbe, W. W., Marque, J., Denk, W., McCray, J. A. and Hess, G. P. Synthesis, photochemistry, and biological activity of a caged photolabile acetylcholine receptor ligand. Biochemistry 28: 49-55 (1989).
Miller, M. J., Wei, S. H., Cahalan, M. D. and Parker, I. Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy. Proceedings of the National Academy of Sciences (USA) 100: 2604-2609 (2003).
Miller, M. J., Wei, S. H., Parker, I. and Cahalan, M. D. Two-photon imaging of lymphocyte motility and antigen response in intact lymph node. Science 296: 1869-1873 (2002).
Moretensen, O. S. and Svendsen, E. N. Initial and final molecular states as "virtual states" in two-photon processes. Journal of Chemical Physics 74: 3185-3189 (1981).
Muller, M., Schmidt, J., Mironov, S. L. and Richter, D. W. Construction and performance of a custom-built two-photon laser scanning system. Journal of Physics D 36: 1747-1757 (2003).
Na, J., Magee, C. and Betzig, E. High-speed, low-photodamage nonlinear imaging using passive pulse splitters. Nature Methods 5: 197-202 (2008).
Nakamura, O. Fundamentals of two-photon microscopy. Microscopy Research and Technique 47: 165-171 (1999).
Neil, M. A. A., Juskaitis, R., Booth, M. J., Wilson, T., Tanaka, T. and Kawata, S. Adaptive aberration correction in a two-photon microscope. Journal of Microscopy 200: 105-108 (2000).
Neilsen, T., Fricke, M., Hellweg, D. and Andresen, P. High efficiency beam splitter for multifocal multiphoton microscopy. Journal of Microscopy 201: 368-376 (2001).
Nguyen, Q. T., Callamaras, N., Hsieh, C. and Parker, I. Construction of a two-photon microscope for video-rate Ca2+ imaging. Cell Calcium 30: 383-393 (2001).
Nikolenko, V., Nemet, B. and Yuste, R. A two-photon and second harmonic microscope.Methods 30: 3-15 (2003).
Nikolenko, V., Poskanzer, K. E. and Yuste, R. Two-photon photostimulation and imaging of neural circuits. Nature methods 4: 943-950 (2007).
Nitsch, R., Pohl, E. E., Smorodchenko, A., Infante-Duarte, C., Aktas, O. and Zipp, F. Direct impact of T cells on neurons revealed by two-photon microscopy in living brain tissue.Journal of Neuroscience 24: 2458-2464 (2004).
Oheim, M., Beaurepaire, E., Chaigneau, E., Mertz, J. and Charpak, S. Two-photon microscopy in brain tissue: parameters influencing the imaging depth. Journal of Neuroscience Methods 111: 29-37 (2001).
Oheim, M., Michael, D. J., Geisbauer, M., Madsen, D. and Chow, R. H. Principles of two-photon excitation fluorescence microscopy and other nonlinear imaging approaches.Advanced Drug Delivery Reviews 58: 788-808 (2006).
Oron, D., Tal, E. and Silberberg, Y. Scanningless depth-resolved microscopy. Optics Express 13: 1468-1476 (2005).
Patterson, G. H. and Piston, D. W. Photobleaching in two-photon excitation microscopy.Biophysical journal 78: 2159-2162 (2000).
Pennisi, E. Photons add up to better microscopy. Science 275: 480-481 (1997).
Periasamy, A., Skoglund, P., Noakes, C. and Keller, R. An evaluation of two-photon excitation versus confocal and digital deconvolution fluorescence microscopy imaging in Xenopusmorphogenesis. Microscopy Research and Technique 47: 172-181 (1999).
Perkins, T. T., Quake, S. R., Smith, D. E. and Chu, S. Relaxation of a single DNA molecule observed by optical microscopy. Science 264: 822-826 (1994).
Perkins, T. T., Smith, D. E. and Chu, S. Direct observation of tube-like motion of a single polymer chain. Science 264: 819-822 (1994).
Peter, M. and Ameer-Berg, S. M. Imaging molecular interactions by multiphoton FLIM.Biology of the Cell 96: 231-236 (2004).
Piston, D. W. When two is better than one: elements of intravital microscopy. PLoS Biology3: 960-962 (2005).
Post, J. N., Lidke, K. A., Rieger, B. and Arndt-Jovin, D. J. One- and two-photon photoactivation of a paGFP-fusion protein in live drosophila embryos. FEBS Letters 579:325-330 (2005).
Potter, S. M. Vital imaging: two photons are better than one. Current Biology 6: 1595-1598 (1996).
Rentzepis, P. M., Mitschele, C. J. and Saxman, A. C. Measurement of ultrashort laser pulses by three-photon fluorescence. Applied Physics Letters 17: 122-125 (1970).
Ruan, Q., Chen, Y., Gratton, E., Glaser, M. and Mantulin, W. W. Cellular Characterization of Adenylate Kinase and Its Isoform: Two-Photon Excitation Fluorescence Imaging and Fluorescence Correlation Spectroscopy. Biophysical Journal 83: 3177-3187 (2002).
Rubart, M. Two-photon microscopy of cells and tissue. Circulation Research 95: 1154-1166 (2004).
Sako, Y., Sekihata, A., Yanagisawa, Y., Yamamoto, M., Shimada, Y., Ozaki, K. and Kusumi, A.Comparison of two-photon excitation laser scanning microscopy with UV-confocal laser scanning microscopy in three-dimensional calcium imaging using the fluorescence indicator Indo-1. Journal of Microscopy 185: 9-20 (1997).
Sanchez, E. J., Novotny, L., Holtom, G. R. and Xie, X. S. Room-temperature fluorescence imaging and spectroscopy of single molecules by two-photon excitation. Journal of Physical Chemistry A 101: 7019-7023 (1997).
Sanchez, S. A. and Gratton, E. Lipid-protein interactions revealed by two-photon microscopy and fluorescence correlation spectroscopy. Accounts of Chemical Research38: 469-477 (2005).
Schneider, M., Barozzi, S., Testa, I., Faretta, M. and Diaspro, A. Two-photon activation and excitation properties of PA-GFP in the 720-920 nm region. Biophysical Journal 89: 1346-1352 (2005).
Schrader, M., Hell, S. W. and van der Voort, H. T. M. Three-dimensional super-resolution with a 4Pi-confocal microscope using image restoration. Journal of Applied Physics 84: 4033-4042 (1998).
Schrader, M., Hofmann, U. G. and Hell, S. W. Ultrathin fluorescent layers for monitoring the axial resolution in confocal and two-photon fluorescence microscopy. Journal of Microscopy 191: 135-140 (1998).
Schrock, E., du Manoir, S., Veldman, T., Schoell, B., Wienberg, J., Ferguson-Smith, M. A., Ning, Y., Ledbetter, D. H., Bar-Am, I., Soenksen, D., Garini, Y. and Ried, T. Multicolor spectral karyotyping of human chromosomes. Science 273: 494-497 (1996).
Schwille, P., Haupts, U., Maiti, S. and Webb, W. W. Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation.Biophysical Journal 77: 2251-2265 (1999).
Schwille, P. and Heinze, K. G. Two-photon fluorescence cross-correlation spectroscopy.ChemPhysChem 2: 269-272 (2001).
Singh, S. and Bradley, L. T. Three-photon absorption in naphthalene crystals by laser excitation. Physical Review Letters 12: 612-614 (1964).
Soeller, C. and Cannell, M. B. Construction of a two-photon microscope and optimization of illumination pulse duration. European Journal of Physiology 432: 555-561 (1996).
Soeller, C. and Cannell, M. B. Two-Photon Microscopy: Imaging in Scattering Samples and Three-Dimensionally Resolved Flash Photolysis Microscopy Research and Technique 47:182-195 (1999).
Sonnleitner, M., Schutz, G. J., Kada, G. and Schindler, H. Imaging single lipid molecules in living cells using two photon excitation. Single Molecules 1: 182-183 (2000).
Sonnleitner, M., Schutz, G. J. and Schmidt, Th. Imaging individual molecules by two-photon excitation. Chemical Physics Letters 221-226: 221-226 (1999).
Squirrell, J. M., Wokosin, D. L., White, J. G. and Bavister, B. D. Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability. Nature Biotechnology 17: 763-767 (1999).
Stosiek, C., Garaschuk, O., Holtohoff, K. and Konnerth, A. In vivo two-photon calcium imaging of neuronal networks. Proceedings of the National Academy of Sciences (USA) 100: 7319-7324 (2003).
Straub, M. and Hell, S. W. Multifocal multiphoton microscopy: a fast and efficient tool for 3-D fluorescence imaging. Bioimaging 6: 177-185 (1998).
Straub, M. and Hell, S. W. Fluorescence lifetime three-dimensional microscopy with picosecond precision using a multifocal multiphoton microscope. Applied Physics Letters73: 1769-1771 (1998).
Stutzmann, G. E. and Parker, I. Dynamic multiphoton imaging: A live view from cells to systems. Physiology 20: 15-21 (2005).
Summers, R. G., Piston, D. W., Harris, K. M. and Morrill, J. B. The orientation of first cleavage in the sea urchin embryo, Lytechinus variegatus, does not specify the axes of bilateral symmetry. Developmental Biology 175: 177-183 (1996).
Svoboda, K., Helmchen, F., Denk, W. and Tank, D.W. Spread of dendritic excitation in layer 2/3 pyramidal neurons in rat barrel cortex in vivo. Nature Neuroscience 2: 65-73 (1999).
Svoboda, K., Tank, D. W. and Denk, W. Direct measurement of coupling between dendritic spines and shafts. Science 272: 716-719 (1996).
Svoboda, K. and Ysuda, R. Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron 50: 823-839 (2006).
Sytsma, J., Vroom, J. M., De Grauw, C. J. and Gerritsen, H. C. Time-gated fluorescence lifetime imaging and microvolume spectroscopy using two-photon excitation. Journal of Microscopy 191: 39-51 (1998).
Taki, M., Wolford, J. L. and O'Halloran, T. V. Emission ratiometric imaging of intracellular zinc: design of a benzoxazole fluorescent sensor and its application in two-photon microscopy. Journal of the American Chemical Society 126: 712-713 (2004).
Tan, Y. P., Llano, I., Hopt, A., Wurriehausen, F. and Neher, E. Fast scanning and efficient photodetection in a simple two-photon microscope. Journal of Neuroscience Methods 92:123-135 (1999).
Tauer, U. Advantages and risks of multiphoton microscopy in physiology. Experimental Physiology 87: 709-714 (2002).
Tsai, P. S., Friedman, B., Ifarraguerri, A. I., Thompson, B. D., Lev-Ram, V., Schaffer, C. B., Xiong, Q., Tsien, R. Y., Squier, J. A. and Kleinfeld, D. All-optical histology using ultrashort laser pulses. Neuron 39: 27-41 (2003).
Veigel, C., Coluccio, L. M., Jontes, J. D., Sparrow, J. C., Milligan, R. A. and Molloy, J. E. The motor protein myosin-I produces its working stroke in two steps. Nature 398: 530-533 (1999).
Vorobjev, I. A., Liang, H., Wright, W. H. and Berns, M. W. Optical trapping for chromosome manipulation: a wavelength dependence of induced chromosome bridges. Biophysical Journal 64: 533-538 (1993).
Wang, J. W., Wong, A. M., Flores, J., Vosshal, L. B. and Axel, R. Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. Cell 112: 271-282 (2003).
Wang, Y., Wang, X. F., Wang, C. and Ma, H. Simultaneously multi-parameter determination of hematonosis cell apoptosis by two-photon and confocal laser scanning microscopy.Journal of Clinical Laboratory Analysis 18: 271-275 (2004).
Williams, R. M., Shear, J. B., Zipfel, W. R., Maiti, S. and Webb, W. W. Mucosal mast cell secretion processes imaged using three-photon microscopy of 5-hydroxytryptamine autofluorescence. Biophysical Journal 76: 1835-1846 (1999).
Wiseman, P. W., Squier, J. A., Ellisman, M. H. and Wilson, K. R. Two-photon image correlation spectroscopy and image cross-correlation spectroscopy. Journal of Microscopy 200: 14-25 (2000).
Wokosin, D. L., Centonze, V. E., Crittenden, S. and White, J. Three-photon excitation fluorescence imaging of biological specimens using an all-solid-state laser. Bioimaging 4:208-214 (1996).
Wokosin, D. L., Centonze, V. E., White, J. G., Armstrong, D., Robertson, G. and Ferguson, A. I.All-solid-state ultrafast lasers facilitate multiphoton excitation fluorescence imaging. IEEE Journal of Selected Topics in Quantum Electronics 2: 1051-1065 (1996).
Xu, C. and Webb, W. W. Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm. Journal of the Optical Society of America 13:481-491 (1996).
Yoder, E. J. and Kleinfeld, D. Cortical imaging through the intact mouse skull using two-photon excitation laser scanning microscopy. Microscopy Research and Technique 56:304-305 (2002).
Zhou, X., Ren, A. M., Feng, J. K., Liu, X. J., Zhang, J. and Liu, J. One-and two-photon absorption properties of novel multi-branched molecules. Phys.Chem.Chem.Phys. 4:4346-4352 (2002).
Zipfel, W. R., Williams, R. M., Christie, R., Nikitin, A. Y., Hyman, B. T. and Webb, W. W. Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proceedings of the National Academy of Sciences (USA)100: 7075-7080 (2003).
Zoumi, A., Dattta, S., Liaw, L. H. L., Wu, C. J., Manthripragada, G., Osborne, T. F. and LaMorte, V. J. Spatial distribution and function of sterol regulatory element-binding protein 1a and 2 homo-and heterodimers by in vivo two-photon imaging and spectroscopy fluorescence resonance energy transfer. Molecular and Cellular Biology 25: 2946-2956 (2005).
Zoumi, A., Yeh, A. and Tromberg, B. J. Imaging cells and extracellular matrix in vivos by using second-harmonic generation and two-photon excited fluorescence. Proceedings of the National Academy of Sciences (USA) 99: 11014-11019 (2002).

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