Source: http://limmshp.iis.u-tokyo.ac.jp/member/researchers/researchers_dco
Timestamp: 2019-04-23 04:32:35+00:00

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LIMMS :: Dr. Dominique COLLARD.
Dominique Collard was born in Cambrai, France in 1958. He received the Eng. Degree from ISEN (Institut Supérieur d'Electronique et du Numérique) in 1980, and the PhD degree from the University of Lille in 1984. From 1985 to 1986, he was with TOSHIBA ULSI research Center in Kawasaki, Japan, as visiting scientist. He entered the Centre National de la Recherche Scientifique (CNRS) as senior researcher in 1988, and settled a research group on process and device simulation at ISEN and IEMN (Institut d’Electronique, de Microélectronique et de Nanotechnologie), Lille, France. In 1995-1997, he was Director of the Laboratory for Integrated Micromechatronic Systems (LIMMS), Tokyo, Japan, a joint CNRS laboratory with the Institute of Industrial Science of the University of Tokyo. Within LIMMS he worked on silicon based electrostatic actuator. From 1997, he is with IEMN, as CNRS research director and settled a silicon micro-system group. November 2000-July 2005, he has a Professor position at the University of Tokyo and was Director of the CIRMM/CNRS (Center of International Research on MicroMechatronics). From August 2005, he joint a second time the Laboratory for Integrated Micromechatronic Systems (LIMMS), Tokyo, Japan where he was appointed director in Sept 2007. In Dec. 2011, he became coordinator of EC/FP7 INCOLAB: EUJO-LIMMS aiming to open LIMMS to European partners and first EC laboratory in Japan. His current scientific interest covers micro and nano systems for applications in biology and nanotechnology.
D. COLLARD and K. TANIGUCHI, “IMPACT, a point defect based two-dimensional process simulator: modeling of lateral oxidation enhanced diffusion of dopants in silicon”, IEEE Trans. Electron Devices, Vol. ED-33, pp. 1454-1462, 1986.
V. SENEZ, D. COLLARD, B. BACCUS, M. BRAULT and J. LEBAILLY, "Analysis and application of viscoelastic model for silicon oxidation", J. Appl. Phys., Vol. 76, pp. 3285-3296, 15 sept. 1994.
T. AKIYAMA, D. COLLARD and H. FUJITA "Scratch Drive Actuator with mechanical links for Self-assembling of three-dimensional MEMS", J. Microelectromechanical Syst., Vol. 6, pp. 10-17,1997.
D. GALAYKO, A KAISER, L. BUCHAILLOT, B. LEGRAND, D. COLLARD, C. COMBI, "Design, realisation and test of micro-mechanical resonators in thick-film silicon technology with postprocess electrode-to-resonator gap reduction", Journal of Micromechanics and Microengineering, Vol. 13, pp. 134-140, Janvier 2003.
V. AGACHE, B. LEGRAND, D. COLLARD, L. BUCHAILLOT, H. FUJITA, " Fabrication and characterization of 1.1 GHz blade nano-electromechanical resonator", Applied Physics Letters, vol 86, p. 213114, 2005.
There have been numerous successful attempts to build functional molecular microfluidic devices. However, issues on robustness, standardization and reliability of the detection mechanism are not cleared. Fluorescence imaging damages molecules preventing from real-time long-period monitoring while immunoassays suffers standardization problem at low concentrations and electrical measurements have performance limitation in buffer environment. Molecular biomechanical detection with the integrated Silicon Nano Tweezers (SNT) is a promising approach as already demonstrated in air and in liquid. However, performing all steps of the experiment in liquid requires very stable and reliable microfluidic integration.
This research proposes using a PDMS channel, placed on the edge of a cover slip, with a lateral opening for SNT tips to enter (Fig.1). After capturing necessary molecules, input solution changes (with an integrated syringe pump) for detection. The result is monitored as electrical signals showing the change in resonance frequency (Res. Freq.) and quality factor (Q-factor). With this integrated SNT with microfluidics we have demonstrated continuous, real-time monitoring of fluid handling and microtubule (MT) capturing. These results pave the way to molecular detection outputting electrical signals, an awaited reliable indicator to standardize the detection.
Fig.1 Schematic view of the developped system. a) SNT was inserted into the channel via the lateral opening of the syringe pump integrated PDMS device. b) The liquid inside the channel was changed with syringe pump. Changes in the resonance frequency and Q-factor monitor MT capturing. The device can then be used for monitoring molecular attachment on MTs.
2. Optimized micro devices for liquid-dielectrophoresis (LDEP) actuation of conductive solutions. (with V. Agache, CEA/LETI, Grenoble, France and Fujita Lab.).
Liquid dielectrophoresis (LDEP) technique is known to displace liquids in open environments by applying alternating or DC voltages between electrodes patterned below a single or a stack of dielectric layers (Fig.2). This technique is able to handle pico–nano liter droplets and could be used to carry out biological and chemical protocols. According to the electrode geometry, the dielectric layers constants and thicknesses, this technique may require relatively high actuation voltages (∼200–500 VRMS). In addition, most of experiments carried out in the literature have shown the LDEP technique to be more adapted to insulating or semi insulating liquids (lower than 1 mS m−1).
This research reports optimization of LDEP devices, in terms of miniaturization (generation of droplets arrays with droplets size ranging from 0.5 pL to 0.1 nL), and choice of dielectric materials. We have successfully addressed two challenging constraints in the ﬁeld of LDEP: ﬁrstly deionized water can be actuated with operating voltages below 150 VRMS and secondly solutions with conductivity up to 10 mS m-1 have been partly displaced. These results allow us to foresee manipulation of organic solutions, biological buffers by LDEP at the micro-nanoscale, which will attract strong interest for sample preparation in complex biochemical protocols.
Fig. 1 (a)–(c) Evolution of a DI water liquid ﬁnger while applying a 250 ms signal pulse of 236 VRMS at 100 kHz. The dielectric layers are made of 300 nm SiN and 300 nm SiOC. (d)–(e) The picoliter droplets are created by the break-up of the capillary instability at semi circular proﬁles positions called bumps.
Fig. 2 Graph illustrating the inﬂuence of the geometry (electrode width w and inter electrode gap g) onto the threshold actuation voltage Vth.
3. Silicon nano tweezers for real time biomechanical assay on dna damage by therapeutic radiation beams (with Center Oscar Lambret, Lille, France and Fujita lab.).
Tumor cell killing by γ-ray beams in cancer radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. On the other hand, the mechanical behavior of DNA, e.g., sequence–sensitivity, elastic vs. plastic response, is well understood. However, manipulations are usually performed by AFM or optical tweezers, instruments that can hardly be placed and operate under radiation beams. This new activity aims to the biomechanical characterization of a λ-DNA bundle exposed to a therapeutic radiation beam by Silicon Nanotweezers (Fig. 3). The device endures the harsh environment of radiation beams and still retains molecular-level accuracy. This result paves the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment.
Fig. 3 (a) CyberKnife machine in Centre Oscar Lambret, Lille, France used for the experiments.
Fig. 3 (b) Experimental set-up. The SNT is under the radiation beam generated by the CyberKnife head. (c) Picture of the SNT showing the grounded connection to the lock-in amplifier (not shown).
The objective of this project is to demonstrate, for the first time, the single molecule manipulation and characterization by micromachined silicon based tweezers. DNA is the first targeted molecule. Molecule isolation is performed thanks to microfluidics device (Fig.1). Dedicated tweezers have been developed with appropriate actuation and sensing characteristics (Fig.2). DNA bundle has been successfully trapped by immersed tweezers, as shown by SEM image of a trapped DNA bundle bridging the tweezers tips (Fig. 3).
Performing biological test by direct molecular manipulation can provide quick and reliable data as noise due to bulk experiment can be reduced. However, these experiments relying on optical or magnetic tweezers have a low throughput since the molecule preparation is done one at a time. To move towards systematic biological or medical analysis, Micro and Nano Systems (MEMS) are the appropriate tools as they can integrate accurate molecular level engineering tools and can be cheaply produced with highly parallel process. This research focuses on this long term goal.
The objective of this project is to demonstrate routine molecules manipulation and systematic bio reaction sensing by Silicon Nano Tweezers (SNT), Fig.1. DNA molecules bundle is trapped by the SNT from DNA solution. The biological reaction is sensed by immersing the trapped bundle in enzyme solution and tracking the mechanical response of the SNT.
DNA bundle is first trapped between the 2 tips of the tweezers, insert of Fig. 1. The bundle handled by the SNT is immersed in the enzymatic solution contained in the reaction cell. The enzymes, Hind III digest the DNA. The mechanical resonance frequency FR and Q factor of the SNT+bundle is extracted in real time as shown in fig. 2. The reduction of FR and increase of the Q factor give the kinetics of the digestion reaction. This first result proves the feasibility of the molecular based bio-sensing with Silicon Nano Tweezers.
Fig. 1 Silicon Nanotweezers and trapped DNA bundle.
Fig. 2 Tweezers + bundle FR and Q vs. Digestion time. Response without proteins is also shown (witnesses).
 M. Kumemura et al., J. Micromech. Microeng, 21 (054020), 2011.
 Collard, Front Biosci, 5 (955), 2013.
Additional documents related to "Scientific Reports, vol. 6, p. 28001, 06/16/online 2016"
S.L. Karsten, M.C. Tarhan, L.C. Kudo, D. Collard, H. Fujita, Point-of-Care Devices (POCDs) by Means of Advanced MEMS, Talanta, 2015 (accepted).
D. Collard, Nano systems and nano scaled devices for new applications in biology and nanotechnology. (invited plenary talk), EUROSOI-ULIS 2015, 2015 Joint International EUROSOI Workshop and International Conference on Ultimate Integration on Silicon, January 26-28, 2015 - Bologna, Italy.
R. Renaudot, Y. Fouillet, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, et al., "Performances of a broad range of dielectric stacks for Liquid Dielectrophoresis transduction," Microfluidics Nanofluidics, vol. 16, pp. 620-626, 2014.
R. Renaudot, Y. Fouillet, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, et al., "Programmable LDEP technology to fabricate versatile master molds for PDMS continuous-flow microfluidic applications," Microfluid Nanofluid, vol. 16, pp. 701-710, 2014.
N. Lafitte, Y. Haddab, Y. Le Gorrec, H. Guillou, L. Jalabert, D. Collard, et al., "Improvement of silicon nanotweezers sensitivity for mechanical characterization of biomolecules using closed- loop control," IEEE/ASME Trans. Mechatron., vol. Accepted, 2014.
D. Collard, N. Lafitte, H. Guillou, M. Kumemura, L. Jalabert, and H. Fujita, "Silicon Nano Tweezers for molecules and cells manipulation and characterization," in Emerging Tools for Micro and Nano Manipulation, X. Liu and Y. Sun, Eds., ed: Wiley-VCH, 2014, p. (in press).
F. Manca, S. Giordano, P.L. Palla, G. Perret, E. Lartigau, D. Collard, H. Fujita, F. Cleri, "Theoretical study of the basic mechanisms of DNA damage by therapeutic radiation beams", E-MRS Spring meeting 2014, Symposium N, May 26-29, Lille (France).
G. Perret, T. Lacornerie, M. Kumemura, H. Guillou, L. Jalabert, E. Lartigau, T. Fujii, F. Cleri, H. Fujita, D. Collard, "Silicon nanotweezers inside liquid for the real time characterization of DNA degradation under radiotherapy treatment", E-MRS Spring meeting 2014, Symposium N, May 26-29, Lille (France).
M. Kumemura, S. L. Karsten, N. Lafitte, H. Guillou, L. Jalabert, H. Fujita, D. Collard, ELECTRICAL DETECTION OF AMPLIFIED DNA USING SILICON NANOTWEEZERS, 7th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT2014), 11-3 (2pages), June 29 - July 2, 2014 / EXCO, Daegu, Korea.
H. Guillou, N. Iwanaka, N. Lafitte, M. Kumemura, K. Shimizu, L. Jalabert, S. Kaneda, T. Fujii, H. Fujita, K. Sakata, S. Konishi, D. Collard, SILICON NANO TWEEZERS WITH FORCE/STIFFNESS/LOSSES MEASUREMENTS OF LOCAL CONTRACTILITY OF MYOCYTES, 7th Asia-Pacific Conference on Transducers and Micro/Nano Technologies (APCOT2014), 11-6 (2pages), June 29 - July 2, 2014 / EXCO, Daegu, Korea.
P-T. Chiang, M. Kumemura , H.Fujita, G. Perret, N. Lafitte, L.Jalabert, D. Collard, A. Enomoto, K. Miyagawa, Real-time Measurement of DNA Degradation under Radiation by Silicon Nanotweezers Coupled with Microfluidic Cavity, Technical Meeting on Sensors and Micromachines 2014.
R. Renaudot, V. Agache, Y. Fouillet, G. Laffite, L. Jalabert, E. Bisceglia, M. Kumemura, D. Collard and H. Fujita, "A programmable and reconfigurable microfluidic chip", Lab Chip,Lab Chip, 2013,13, 4517-4524.
R. Renaudot, Y. Fouillet, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, V. Agache “Programmable LDEP technology to fabricate versatile master molds for PDMS continuous-flow microfluidic applications”, Microfluidics and Nanofluidics, Sept. 2013.
R. Renaudot, V. Agache, Y. Fouillet, M. Kumemura, L. Jalabert, D. Collard, H. Fujita, Performances of a broad range of dielectric stacks for liquid dielectrophoresis transduction, Microfluidics Nanofluidics, 2013.
G. Perret, T. Lacornerie, M. Kumemura, N. Lafitte, H. Guillou, L. Jalabert, E. Lartigau, T. Fuji, F. Cleri, H. Fujita, D. Collard, Real Time Biomechanical Characterization of DNA Damage under Therapeutic Radiation Beams, MRS Boston, Dec. 3rd 2013 (Oral).
M. Kumemura, S. L. Karsten, N. Lafitte, H. Guillou, L. Jalabert, H. Fujita, D. Collard, Isothermal amplification of DNA on tips of silicon nanotweezers and its electrical and mechanical characterizations, MicroTAS 2013, October 27-31, 2013, Freiburg, Germany.(oral).
N. Lafitte, Y. Haddab, Y. Le Gorrec, H. Guillou, M. Kumemura, L. Jalabert, H. Fujita, D. Collard, Closed-loop control of silicon nanotweezers for improvement of sensitivity to mechanical stiffness measurement and bio-sensing on DNA molecules, 2013 IEEE/RSJ International Conference on Intelligent Robots and Systems (Oral).
N. Lafitte, Y. Haddab, Y. Le Gorrec, H. Guillou, M. Kumemura, L. Jalabert, D. Collard and H. Fujita, ACTIVE CONTROL OF SILICON NANOTWEEZERS DETECTS ENZYMATIC REACTION AT THE MOLECULAR LEVEL, IEEE Transducers 2013, June, Barcelona (Spain). (poster).
Dominique Collard, Thomas Lacornerie, Momoko Kumemura, Nicolas Lafitte, Herve Guillou, Laurent Jalabert, Eric Lartigau, Teruo Fujii, Fabrizio Cleri, Hiroyuki Fujita, "SILICON NANO TWEEZERS FOR REAL TIME BIOMECHANICAL ASSAY ON DNA DAMAGE BY THERAPEUTIC RADIATION BEAMS" (Oral), MicroTAS, Okinawa, JAPAN, Oct. 28 – Nov. 1, 2012.
M. C. Tarhan,1 D. Collard, L. Jalabert, M. Kumemura, N. Lafitte, Q. Delouvee, S. L. Karsten and H. Fujita, "CONTINUOUS REAL-TIME MONITORING OF MOLECULAR DETECTION BY SILICON NANOTWEEZERS-INTEGRATED MICROFLUIDIC DEVICE", MicroTAS, Okinawa, JAPAN, Oct. 28 – Nov. 1, 2012.
Y. Tauran, M. Kumemura, N. Lafitte, R. Ueno, L. Jalabert, Y. Takayama, D. Collard, H. Fujita, A. W. Coleman and B-J. Kim, ‘Mechanical effect of calix[n]arene capped silver nanoparticles on DNA measured with silicon nano tweezers’, MicroTAS, Okinawa, JAPAN, Oct. 28 – Nov. 1, 2012.
R. Renaudot, V. Agache, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, Performances of High-K dielectric materials (Al2O3, HfO2, ZrO2) for Liquid Dielectropphoresis (LDEP) microfluidic devices, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2012), 28 Oct-1 Nov, Okinawa, Japan.
R. Renaudot, Y. Fouillet, V. Agache, L. Jalabert, M. Kumemura, D. Collard, H. Fujita, New approach for continuous based-channels microfluidic chips fabrication using EWOD and/or LDEP transductions, 8th International Meeting on Electrowetting, 21-23 June 2012, Athens, Greece. Best poster award.
M. C. Tarhan, R. Yokokawa, L. Jalabert, D. Collard and H. Fujita, A MOTOR PROTEIN-BASED ENZYMATIC DETECTION SYSTEM, accepted to MicroTAS 2011.
N. Lafitte, M. Kumemura, L. Jalabert, D. Collard and H. Fujita, REAL-TIME SENSING OF MOLECULE BINDING ON DNA WITH SILICON NANOTWEEZERS, accepted to MicroTAS 2011.
Y-A. Chapuis, L. Jalabert, E. Sarajlic, D. Collard, H. Fujita, Polymer Membrane-based thermo-pneumatic actuation for distributed air-jet planar micromanipulator, Accepted as an oral presentation at IEEE Int. Conference on MEMS 2011, Cancun, Mexico.
M. Kumemura, D. Collard, R. Tourvielle, N. Lafitte, K. Montagne, S. Yoshizawa, D. Fourmy, L. Jalabert, Y. Sakai, S. Takeuchi, T. Fujii, and H. Fujita, INTEGRATED MEMS PLATFORM WITH SILICON NANOTWEEZERS AND OPEN MICROFLUIDIC DEVICE FOR MOLECULAR AND CELLULAR BIOMECHANICAL ASSAYS, Accepted as an oral presentation at IEEE Int. Conference on MEMS 2011, Cancun, Mexico.
B. Daunay, P. Lambert, L. Jalabert, D. Collard and H. Fujita, Optimization of liquid dielectrophoresis (L-DEP) based devices towards conductive biological liquids handling, Accepted in Transducers 2011, Beijin.
N. Lafitte, M. Kumemura, D. Collard, R. Tourvielle, K. Montagne, S. Yoshizawa, D. Fourmy, L. Jalabert, Y. Sakai, S. Takeuchi, T. Fujii and H. Fujita, “Silicon nanotweezers for molecular and cellular biomechanical assays,” The 6th International Conference on Microtechnologies in Medicine and Biology, Lucerne, Switzerland, MMB 2011.
MC. Tarhan, R. Yokokawa, L. Jalabert, D. Collard, H. Fujita, Biomotor-Based Nanotransport System Constructed by Pick-And-Place Assembly of Individual Molecule, The 2010 IEEE/RSJ International Conference on Intelligent RObots and Systems, Taipei International Convention Center, Taipei, Taiwan, October 18-22, 2010.
N. Lafitte, M. Kumemura, D. Collard, S. Yoshizawa, D. Fourmy, L. Jalabert, S. Takeuchi, T. Fujii, H. Fujita, Direct bio-mechanical sensing analysis of enzymatic reactions on DNA by silicon nanotweezers, Technical Meeting on Micromachine and Sensor Systems, IEE Japan, 2010, June 17-18, Tokyo.
N. Lafitte, M. Kumemura, M. Nagai, L. Jalabert, D. Collard and H. Fujita, “An open microfluidic device with acrive valves for accurate trapping of DNA by silicon nanotweezers," Proc. of 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp. 1865-1867, Groningen, The Netherlands, μTAS 2010.
N. Lafitte, M. Kumemura, D. Collard, S. Yoshizawa, D. Fourmy, L. Jalabert, S. Takeuchi, T. Fujii and H. Fujita, “Direct bio-mechanical sensing of enzymatic reaction on DNA by silicon nanotweezers," IEJ Workshop management system, The papers of technical meeting on Micromachine and Sensor System, The Institute of Electrical Engineers of Japan, pp.127-131, Tokyo 2010.
M. Kumemura, D. Collard, S. Yoshizawa, D. Fourmy, N. Lafitte, L.Jalabert, S. Takeuchi, T. Fujii, H. Fujita, DIRECT BIO-MECHANICAL SENSING OF ENZYMATIC REACTION ON DNA BY SILICON NANOTWEEZERS, IEEE Int. Conf. on MEMS 2010, Hong Kong.
Yamahata C.,Sarajic E.,Collard D.,Jalabert L.,Kumemura M. and Fujita H., Mechanical characterization of biomolecules in liquid using silicon tweezers with subnanonewton resolution, IEEE 22th Int. Conf. on Micro Electro Mechanical Systems, MEMS 09, Sorrento, Italy, 607-610, 2009.
Bottier, C.; Tarhan, M. C.; Collard, D.; Yokokawa, R.; Fujita, H. Kinesin-based Transportation and Electrofusion of Lipid Vesicles, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS'08, San Diego, USA, Oct. 12-16, 2008.
Sarajlic, E.; Yamahata, C.; Cordero, M.; Collard, D.; Fujita, H. HAREM: High Aspect Ration Etching and Metallization, 21st IEEE Int. Conf. on Microelectromechanical Systems, MEMS'08, Tucson, USA, Jan. 13-17, 2008.
Tarhan, M. C.; Collard, D.; Bottier, C.; Yokokawa, R.; Hosogi, M.; Hashiguchi, G.; Fujita, H. Isolation and Manipulation of Single Microtubule by Silicon Microtweezers, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS'08, San Diego, USA, Oct. 12-16, 2008.
Wee, B.; Kumemura, M.; Collard, D.; Fujita, H. Isolation of Single DNA Molecule in Picolitre-sized Droplet Formed by Liquid Dielectrophoresis, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS'08, San Diego, USA, Oct. 12-16, 2008.
Wee, B.; Kumemura, M.; Collard, D.; Fujita, H. Integration of Closed Microfluidics and Open Droplet Actuation on a Single Lab-on-a-chip Device, Intelligence and Precision Equipment IIP 2008, Tokyo, Japan, 2008.
Wee, B.; Kumemura, M.; Collard, D.; Fujita, H. Integration of Microfluidics and Droplet Manipulation of DNA Solution on a Single Lab-on-a-chip Device, 4th Asia-Pacific Conference on Transducers and Micro-Nano Technology, APCOT'08, Tainan, Taiwan, Jun. 22-25, 2008.
Wee, B.; Kumemura, M.; Collard, D.; Fujita, H. Droplet Extraction from PDMS Surface using Liquid Dielectrophoresis, International Symposium on Surface Science and Nanotechnology, 2008.
Yamahata, C.; Collard, D.; Domenget, A.; Hosogi, M.; Kumemura, M.; Hashiguchi, G.; Fujita, H. Silicon Nanotweezers: a New Biophysical Tool for Molecular Experimentation, 21st IEEE Int. Conf. on Microelectromechanical Systems, MEMS'08, Tucson, USA, Jan. 13-17, 2008.
Yokokawa, R.; Tarhan, M. C.; Bottier, C.; Collard, D.; Fujita, H.; Kasahara, M. DNA Molecule Manipulation by Motor Proteins and Enzymatic Reaction at the Single Molecule Level, 25th Sensor Symposium on Sensors, Micromachines, and Applied Systems, Okinawa, Japan, Oct.22-24, 2008.
M. Kumemura, D. Collard, C. Yamahata, N. Sakaki, G. Hashiguchi, H. Fujita, "Isolation of DNA molecule in microchannel and a single molecule trapping between microelectrodes", 11th Int Conf. on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 07, 2007, Paris, France.
N. SakakiM. Kumemura, D. Collard, G. Hashiguchi, H. Fujita, "Trapping of single DNA molecule by MEMS tweezers with pulsed dielectrophoresis", 11th Int Conf. on Miniaturized Systems for Chemistry and Life Sciences, MicroTas 07, 2007, Paris, France.
E.Sarajlic, D. Collard, H. Toshiyoshi, H. Fujita, "12-bit micromechanical digital to analog converter of displacement: Design, Fabrication and Characterization", 20th IEEE Int Conf. on Microelectromechanical Systems MEMS 07, 2007, Kobe, Japan, Jan. 21-25.
M. Kumemura, C. Yamahata, N. Sakaki, G. Hashiguchi, D. Collard and H. Fujita, "Measurement and modeling of DNA motion in microchannel including frequency dependent electrophoresis and electro-osmotic flow", accepted at Microtas 2006, Nov. 5-9, Tokyo, Japan, 2006.

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