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Timestamp: 2019-04-25 20:21:39+00:00

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Photosynthetic reaction center (RC) of Rhodobacter sphaeroides, due to the availability of its crystal structure, accessibility of site specific mutants and possibility to trigger the single enzyme turnovers by short flashes of light, is a paradigm study object in biophysics. We have tried to understand how ubiquinone is reduced and protonated to ubiquinol in this enzyme. By combining spectroscopic and electrometric measurements with kinetic modeling and molecular dynamics simulations we obtained a mechanistic scheme where the relocations of the ubiquinone ring in its binding pocket were kinetically related to the two electron and two proton transfer steps.
Reduction of the secundary quinone in the Reaction centre of Rhodobacter sphaeroides.
3. Kozlova,M.A., D.A.Cherepanov, L.Baciou, P.Sebban, and A.Y.Mulkidjanian. 2005. Electron transfer coupled protein relaxation in reaction centers of Rb. sphaeroides. In Photosynthesis: Fundamental aspects to global perspectives. A.van der Est and D.Bruce, editors. Montreal. 222-224.
16. Cherepanov, D.A. and A.Y. Mulkidjanian. 1998. Effect of dielectric relaxation on the kinetics of electron transfer in photosynthetic reaction centers. In Photosynthesis: Mechanisms and Effects. G. Garab, editor. Kluwer Academic Publishers, Dordrecht. 795-798.
17. Cherepanov, D.A., W. Drevenstedt, L.I. Krishtalik, A.Y. Mulkidjanian, and W. Junge. 1998. Protein relaxation and kinetics of P680+ reduction in photosystem II. In Photosynthesis: Mechanisms and Effects. G. Garab, editor. Kluwer Academic Publishers, Dordrecht. 1073-1076.
18. Gopta, O.A., D.A. Cherepanov, W. Junge, and A.Y. Mulkidjanian. 1998. Proton transfer from the bulk to the secondary quinone acceptor in chromatophores of Rhodobacter sphaeroides. In Photosynthesis: Mechanisms and Effects. G. Garab, editor. Kluwer Academic Publishers, Dordrecht. 869-872.
23. Cherepanov, D.A., M. Haumann, W. Junge and A.Mulkidajnian. 1995. Topology of chromophores and cofactors in PSII as derived from the local electrochromic bandshifts. p. 531-534, In: Photosynthesis: From Light to Biosphere, Vol. I., Kluver Academic Publishers, Dordrecht.
24. Mulkidjanian, A. , G. Venturoli, A. Hoechkoepler, D. Zannoni, B. A. Melandri, and L. Drachev. 1994. Photosynthetic electrogenic events in native membranes of Chloroflexus aurantiacus: Flash-induced charge displacements within the reaction center-cytochrome c554 complex. Photosynth. Res. 41:135-143.
26. Venturoli, G. , A. Mulkidjanian, A. Hochkoepler, M. Trotta, R. Feick, B. A. Melandri and D. Zannoni. 1992. Studies on the quinone acceptor complex in the photosynthetic reaction center of the termophilic bacterium Chloroflexus aurantiacus. p. 161-163. In; Thermophils: Science and Tecnology. Reikjavik.
29. Verkhovskii, M. I., L. A. Drachev, M. D. Mamedov, A. Y. Mulkidjanyan, A. Y. Semenov, and V. P. Shinkarev. 1990. The electrogenic step associated with the secondary acceptor semiquinone formation in the reaction centers of purple bacteria. Biokhimiya 55:387-394.
30. Verkhovsky, M. I., N. P. Grishanova, M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov, and V. P. Shinkarev. 1990. The mechanism of interaction between the photosynthetic reaction center and cytochrome-bc1 complex in chromatophores from the non-sulphur purple bacterium Rhodobacter sphaeroides. Biokhimiya 55:1202-1209.
31. Shinkarev, V. P., A. L. Drachev, M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov and M. I. Verkhovsky. 1990. Light-induced electron transfer and electrogenic reactions in the cytochrome-bc1 complex of photosynthetic purple bacterium Rhodobacter sphaeroides. p. 393-400. In Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria (G. Drews and E. A. Dawes, eds.). Plenum Press, New York.
34. Verkhovsky, M. I., L. A. Drachev, M. D. Mamedov, A. Y. Mulkidjanian, A. Y. Semenov, and V. P. Shinkarev. 1989. Electrogenic protonation of the reaction center ubiquinone in chromatophores of non-sulphur purple bacteria. Dokl. Akad. Nauk SSSR 304:209-212.
37. Mulkidjanian, A. Y., M. I. Verkhovsky, V. P. Shinkarev, V. D. Sled', N. P. Grishanova, and B. S. Kaurov. 1985. Study of photosynthetic electron transfer reactions by redox probes: Electron transport in non-sulphur purple bacteria. Biokhimiya 50:1786-1796.
38. Shinkarev, V. P., A. Y. Mulkidjanian, M. I. Verkhovsky, and B. S. Kaurov. 1985. Investigation of the kinetic and thermodynamic properties of the photosynthetic reaction center secondary acceptor in chromatophore membrane of nonsulphur purple bacteria. Biol. Membrany 2:725-737.
39. Sled', V. D., A. Y. Mulkidjanian, V. P. Shinkarev, M. I. Verkhovsky, B. S. Kaurov, and A. B. Rubin. 1984. Direct spectrophotometric determination of the midpoint potential of primary and secondary quinone acceptors of photosynthetic reaction center. Biokhimiya 49:204-208.
41. Shinkarev, V. P., M. I. Verkhovsky, V. D. Sled', A. Y. Mulkidjanian, A. V. Zherdev, D. A. Cherepanov and B. S. Kaurov. 1984. Light-induced electron transfer, proton consumption and transmembrane potential difference formation in chromatophores of non-sulphur purple bacteria. p. 157-163. In Solar Energy Bioconversion. (I. V. Berezin, ed.), Pushchino.
42. Sled', V. D., M. I. Verkhovsky, V. P. Shinkarev, A. Y. Mulkidjanian, N. P. Grishanova, and B. S. Kaurov. 1983. Interactions of redox-mediators with chromatophores of photosynthetic bacterium Rhodospirillum rubrum. Molek. Biologiya 17:33-41.

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