Source: https://exchange.ifmsa.org/exchange/score/explore/projects/view/5857
Timestamp: 2019-04-25 22:36:32+00:00

Document:
Effects of sodium overload in post natal phase in rats on the hydroelectrolytic balance.
Plasma osmolality changes can be too dangerous to a healthy life. Many studies have showed that hyperosmolality can influence blood pressure and inflammation process. Vidonho et al (2004) showed that offspring of mothers that was exposed to a high salt diet during pregnancy and lactation presented high blood pressure levels in adult life. However, the effects of this diet in young life phase, after lactation, is poorly understood yet.
What is the aim of the project? We investigate how overload of salt in the young life of rats, after lactation, could impact the water and sodium intake and urinary excretion in adult phase.
What techniques and methods are used: We use Wistar male rats and exposed as the only fluid source a 0,3 M NaCl solution after lactation (21 days) during 60 days. After the treatment, the animals are kept in a recovery period (15 days) with tap water. After that they are maintained in individual cages with water and 0,3 M NaCl bottles, and the fluid are measured daily. After 5-7 days, dehydration protocols are performed to investigate hydroelectrolytic balance. The animals are allocated in metabolic cages and an intragastric 2 M NaCl load (2 mL, an intracellular dehydration) are performed by gavage. One hour later, glass burettes contained water and 0,3M NaCl were offered and the intake was measured by 2 hours. Urinary salmples are collected before and after fluid intake. A week later, other protocol are performed, 24 hours of water deprivation (an extracellular dehydration). The animals are kept in metabolic cages during 24 hours without access to water, after this period an urinary sample is collected and water and 0,3 M NaCl burettes are offered and the intake is measured through 2 hours. At the end another urinary sample is collected. Urinary volume, osmolality and sodium excretion are calculated using an osmometer and a flame photometer.
Being open to new culture, discussing subjects related to the project and the area of research in the laboratory, being an extrovert.
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