6,4 GRAMS OF COLOURING MATTER OF 50 % CONCENTRATION .
( 2 ) EEC NO E 131 , SCHULZ NO 826 .
THE DEFINITION OF PATENTED BLUE V IS GIVEN IN THE COUNCIL DIRECTIVE ON THE APPROXIMATION OF RULES OF MEMBER STATES ON COLOURING MATTERS FOR USE IN FOODSTUFFS INTENDED FOR HUMAN CONSUMPTION ( OJ NO 115 , 11 . 11 . 1962 , P . 2645/62 ) .
PATENTED BLUE V OF 50 % CONCENTRATION IS MARKED IN THE FEDERAL REPUBLIC OF GERMANY AS " LEBENSMITTELBLAU NR . 3 " .
ANNEX IV
TEST OF THE BITTERNESS OF LUPINS
TO BE PERFORMED ON A SAMPLE OF 200 GRAINS TAKEN FROM A BATCH OF 1 KILOGRAM FROM EACH LOT OF 20 TONNES MAXIMUM
THE BITTER PRINCIPLE SHOULD BE ASSESSED ON THE SEED SENT IN FOR TESTING . THE TEST SHOULD BE RESTRICTED TO THE QUALITATIVE PROOF OF BITTER GRAINS IN THE SAMPLE . THE HOMOGENEITY TOLERANCE SHOULD BE ONE GRAIN IN 100 GRAINS . THE GRAIN-CUT METHOD AFTER V . SENGBUSCH ( 1942 ) , IVANOV AND SMIRNOVA ( 1932 ) AND EGGEBRECHT ( 1949 ) IS APPLICABLE AS THE TESTING METHOD TO LUPINUS ALBUS , LUPINUS AUGUSTIFOLIUS AND LUPINUS LUTEUS . THE DRY OR SWOLLEN GRAINS ARE CUT TRANSVERSELY . THE GRAIN HALVES ARE DIPPED ON A SIEVE FOR 10 SECONDS IN AN IODINE SOLUTION AND THEN RINSED FOR FIVE SECONDS WITH WATER . THE CUT SURFACES OF THE BITTER GRAINS DISCOLOUR TO BROWN BUT THOSE OF THE GRAINS LOW IN ALKALOIDS REMAIN YELLOW .
FOR THE PREPARATION OF THE IODINE SOLUTION , 14 G OF POTASSIUM IODATE ARE DISSOLVED IN AS LITTLE WATER AS POSSIBLE , 10 G OF IODINE IS ADDED AND MADE UP WITH WATER TO 100 CM3 . THE SOLUTION MUST BE LEFT FOR ONE WEEK BEFORE IT MAY BE USED . IT MUST BE STORED IN BROWN BOTTLES . THIS MAIN SOLUTION IS DILUTED BETWEEN 1 : 3 AND 1 : 5 BEFORE USE .