Patent Abstract:
one side - effect arising from the use of antibodies against tnf receptor family members as therapeutics can be liver damage which precludes the completion of clinical trial . a novel lt - dependent pathway is described that mediates liver cell injury in several disease models .

Detailed Description:
activated t cell - induced liver injury in viral and autoimmune hepatitis involves multiple tnfsf members and various signaling pathways . tempering such injury by a single treatment has been difficult . relieving the staggering worldwide burden of liver diseases requires new understanding of pathogenesis and new treatments . the multiple dimensions of ltβr ( and tnfr ) signaling revealed its focal responsibility for integrating distinct hepatocyte injury signaling pathways . the dependence of distinct hepatitis models on ltβr and tnfr signaling was shown ; murine hepatitis was induced by stimulation of tnfsf members fas , cd137 , light , or even cona , a general t cell activator . surprisingly , use of genetic knockout and soluble protein systems revealed that relevant ltβr signaling in cona pathogenesis depends on membrane lt complex signaling directly on hepatocytes . tnfr and ltβr - dependent pathogenic mechanisms involve both the induction of hepatocyte apoptosis and the regulation of inflammation by neutrophil recruitment . a single treatment with soluble ltβr fusion protein ( ltβr - ig ) blocked liver injury from a variety of insults encompassing multiple signaling pathways . these new insights open a new dimension for achieving dynamic control of hepatic immune pathology with treatment implications for hepatitis and multiple forms of liver injury . a recent study using a monoclonal antibody that limited light binding to ltβr , while minimally affecting the binding of lt , suggested an independent role of light in the pathogenesis of hepatitis , presumably acting through ltβr ( anand et al ., 2006 ). in contrast , an independent study suggested light works through hvem signaling in cona - induced hepatitis ( an et al ., 2006 ). however , hvem - deficient mice show a greater susceptibility to cona - induced inflammation that calls into question the importance of hvem signaling in light - mediated hepatitis ( wang et al ., 2005 ). ltβr signaling from the surface lt ligand has a non - redundant role in the development of hepatitis . furthermore , analysis of conditional ltβr - deficient mice was interpreted to mean that ltβr expression specifically on hepatocytes is essential for cona - induced liver injury . lt - ltβr - dependent pathogenic mechanism likely involves both regulation of inflammation by neutrophil recruitment and induction of hepatocyte apoptosis . the role of ltβr in neutrophil recruitment is novel and intriguing since no defect in neutrophil development is reported in lt - or ltβr - deficient mice . gene microarray analysis revealed that expression of several neutrophil specific genes , such as myeloperoxidase and lactoferrin , were reduced in ltα −/− spleen compared to wt mice ( shakhov et al ., 2000 ). reduced numbers of neutrophils in the livers of ltβr - deficient mice following cona administration compared to wt mice and increased numbers when ltβr was stimulated with adenovirus - delivered light have been observed . cxcl2 are ccl3 are potent chemokines controlling neutrophil recruitment to the liver ( nakamura et al ., 2001 ; ramos et al ., 2006 ; bajt et al ., 2001 ; rawaiah et al ., 2007 ). previous studies showed that blocking cxcl2 or ccl3 with specific antibodies or inhibitors effectively protected mice from cona - induced hepatitis ( ajuebar et al ., 2004 ; okamoto et al ., 2005 ). the present disclosure supports that ltβr signaling regulates cxcl2 and ccl3 expression thus promoting neutrophil recruitment to the liver after cona administration . how ltβr signaling promotes hepatocyte apoptosis is not entirely clear . in contrast to tnfri or fas receptors , ltβr lacks the death effector domains known to trigger caspase activation and apoptosis ( ware , 2005 ). however , there is evidence that light - induced ltβr signaling can rapidly recruit traf3 , traf2 , ciap1 , and smac adaptors ( kuai et al ., 2003 ; kim et al ., 2005 ). traf3 activation via ltβr signaling appears to induce apoptosis in some adenocarcinoma cell lines , whereas traf2 signaling promotes nf - κb and jnk activation ( kim et al . 2005 ). several recent studies suggest that traf3 inhibits the noncanonical nf - κb activation pathway by suppressing p100 processing through induction of nik degradation . another report shows pretreatment of human primary hepatocytes with light induces nf - κb activation and blocks apoptosis induced by tnf / actinomycin - d but not fas - induced apoptosis . cell context and balance between nf - kb and apoptotic pathways all appear to influence cell fate . furthermore , ltβr −/− mice were protected from fas and cona - induced liver injury , suggesting collaboration of ltβr and fas signaling pathways leading to liver injury . ltβr - dependent up - regulation of neutrophil recruiting cxcl2 and ccl3 chemokines after fas and cona stimulation promotes liver injury . in line with this , both cxcl2 and ccl3 are known to be transcriptionally activated by nf - kb signaling , which is induced following fas and ltβr stimulation . in contrast to a hepatitis model , previous work by the inventors demonstrated that ltβr signaling promotes liver regeneration after partial hepatectomy . these similar seemingly paradoxical relationships exist for other members of the tnf superfamily of cytokines , fas and tnf for instance . injection of fas agonistic antibody induced massive liver injury in wt , but not in tnfri / ii −/− mice , while the same treatment promoted liver regeneration after partial hepatectomy . ltβr signaling may be constantly maintained by lt and such tuning will coordinate with other tnfrsf members to control homeostasis of liver regeneration and injury . tnf , ifn - γ , and fasl are all induced after cona administration . therefore , it is possible that stimulation of hepatocyte ltβr by lt - expressing t and nkt cells causes apoptosis depending upon the presence of inflammatory cytokines such as tnf , ifnγ , fasl . therefore , inhibitors of tnf or lt , such as soluble receptors for tnf or lt , could be used as a treatment to reduce liver injuries by rather broad insults . an integrative role of ltβr ( and tnfr ) likely exists in connecting various pathways that control hepatocyte apoptosis and survival . understanding the mediators of t cell - mediated liver injury provides targets for difficult - to - treat human liver diseases such as autoimmune and viral hepatitis inhibition of ltβr signaling is effective in preventing both acute cona - induced fulminant hepatitis and a subacute hepatitis induced by cd137 stimulation in fas - deficient mice . in contrast , experimental approaches using stimulation of ltβr signaling are being pursued to improve liver regeneration , or anti - tumor therapy . selective elimination of ltβr “ side effects ”, such as neutrophil - induced liver inflammation may improve efficacy of these treatments . this could also be achieved through inhibition of cxcl2 and cll3 activity using neutralizing antibodies . current therapeutics in development that may result in hepatic liver damage that could be alleviated by inhibition or antagonism of the tnf and / or lt pathways include bms - 663513 , a cd137 agonistic antibody . other antibody therapies that may result in liver damage that could be alleviated by inhibition or antagonism of the tnf and or lt pathways include those that target coinhibitory and costimulatory t cell receptors . coinhibitory and costimulatory molecules include cytotoxic t - lymphocyte antigen - 4 ( ctla - 4 ), glucocorticoid - induced tumor necrosis factor family receptor ( gitr ), b7 - h1 , programmed death [ pd ]- 1 , b7 - h3 and b7x . agonistic antibodies for cd137 are being developed for the treatment of cancer , autoimmune and other immune related diseases , viral disease , by enhancing the response to vaccines and alleviating inflammatory disease . cancer broadly refers to cellular - proliferation and / or cellular growth disease states . the cancer may be breast , prostate , ovarian , brain , melanoma , colorectal , liver , lymphoma , lung , oral , head , neck , spleen , lymph node , small intestine , large intestine , blood cells , stomach , pancreatic , endometrium , testicle , skin , esophagus , bone marrow , blood , cervical , bladder , ewing &# 39 ; s sarcoma , thyroid , a glioma , and / or gastrointestinal cancers . cancer also includes but is not limited to : sarcoma , myxoma , rhabdomyoma , fibroma , lipoma and teratoma , bronchogenic carcinoma , alveolar ( bronchiolar ) carcinoma , bronchial adenoma , chondromatous hamartoma , mesothelioma , squamous cell carcinoma , colorectal adenocarcinoma , leiomyosarcoma , carcinoma of the stomach , pancreatic ductal adenocarcinoma , insulinoma , glucagonoma , gastrinoma , pancreatic carcinoid tumors , vipoma , cancers of the small bowel cancers of the large bowel , colorectal adenocarcinoma , kidney adenocarcinoma , wilm &# 39 ; s tumor , nephroblastoma , bladder and urethra carcinomas , prostate adenocarcinoma and sarcoma , testicular cancers , hepatoma , hepatocellular carcinoma , cholangiocarcinoma , hepatoblastoma , angiosarcoma , hepatocellular adenoma , hemangioma , osteosarcoma , fibrosarcoma , malignant fibrous histiocytoma , chondrosarcoma , malignant lymphoma ( reticulum cell sarcoma ), multiple myeloma , malignant giant cell tumor chordoma , osteochrondroma ( osteocartilaginous exostoses ), benign chondroma , chondroblastoma , chondromyxofibroma , osteoid osteoma and giant cell tumors ; granuloma , xanthoma , osteitis defornians , meningioma , meningiosarcoma , gliomatosis , astrocytoma , medulloblastoma , glioma , ependymoma , germinoma ; pinealoma , glioblastoma multiformae , oligodendroglioma , schwannoma , retinoblastoma , congenital tumors , neurofibroma , breast cancer , endometrial carcinoma , cervical carcinoma , pre - tumor cervical dysplasia , ovarian carcinoma ; serous cystadenocarcinoma , mucinous cystadenocarcinoma , unclassified carcinoma ; granulosa - theca cell tumors , sertoli leydig cell tumors , dysgerminoma , malignant teratoma , vulval cancer , vaginal cancer fallopian tube carcinoma , chronic and acute myeloid leukemia , acute lymphoblastic leukemia , chronic lymphocytic leukemia , myeloproliferative diseases , multiple myeloma , myelodysplastic syndrome , hodgkin &# 39 ; s disease , non - hodgkin &# 39 ; s lymphoma , malignant lymphoma , endothelioma , malignant melanoma , basal cell carcinoma , squamous cell carcinoma , kaposi &# 39 ; s sarcoma , moles dysplastic nevi , lipoma , angioma , dermatofibroma , keloids , psoriasis , and neuroblastoma . the invention is applicable to other cancers discussed herein , including pre - cancers . immune related diseases include systemic lupus erythematosis , rheumatoid arthritis , osteoarthritis , juvenile chronic arthritis , spondyloarthropathies , systemic sclerosis , idiopathic inflammatory myopathies , sjögren &# 39 ; s syndrome , systemic vasculitis , sarcoidosis , autoimmune hemolytic anemia , autoimmune thrombocytopenia , thyroiditis , diabetes mellitus , immune - mediated renal disease , demyelinating diseases of the central and peripheral nervous systems such as multiple sclerosis , idiopathic demyelinating polyneuropathy or guillain - barré syndrome , and chronic inflammatory demyelinating polyneuropathy , hepatobiliary diseases such as infectious , autoimmune chronic active hepatitis , primary biliary cirrhosis , granulomatous hepatitis , and sclerosing cholangitis , inflammatory bowel disease , gluten - sensitive enteropathy , and whipple &# 39 ; s disease , autoimmune or immune - mediated skin diseases including bullous skin diseases , erythema multiforme and contact dermatitis , psoriasis , allergic diseases such as asthma , allergic rhinitis , atopic dermatitis , food hypersensitivity and urticaria , immunologic diseases of the lung such as eosinophilic pneumonias , idiopathic pulmonary fibrosis and hypersensitivity pneumonitis , transplantation associated diseases including graft rejection and graft - versus - host - disease . in addition to soluble receptors for tnf , a wide variety of inhibitors or antagonists of tnf are contemplated in this patent for reducing liver injury induced by various agents , including cd137 . these tnf inhibitors include infliximab ( remicadea ®), mouse - human chimeric anti - hutnf mab ; d2e7 ( humira ™), fully human anti - hutnf mab ; etanercept ( enbrel ®), p75stnf - rii - fc ( dimeric ); peg - p55stnf - ri ( monomeric ); lenercept , p55stnf - ri - igg1 ( dimeric ), cdp571 ( cdr - grafted anti tnf ab ) and cdp870 / certolizumab ( peg - linked anti tnf fab ). other inhibitors of tnf include small molecules ( e . g ., those described by he et al ., 2005 science 310 : 1022 - 1025 ; haraguchi et al ., aids res ther . 2006 ; 3 : 8 . published online 2006 mar . 31 . doi : 10 . 1186 / 1742 - 6405 - 3 - 8 ; strachan et al ., 2000 j . immunol . 164 : 6560 - 6565 ; and u . s . patent application ser . no . 10 / 833 , 871 entitled “ preparation of hymenialdisine derivatives and use thereof .” other tnf inhibitory antibodies include those described in u . s . pat . no . 7 , 160 , 542 ; u . s . patent application ser . nos . 10 / 043 , 436 , and 11 / 180 , 219 inhibitors of tnf , inhibitors of tnf include soluble tnf receptor polypeptides or inhibitors of tnf receptors . other inhibitors include competitive tnf antagonists , including those described in u . s . pat . no . 5 , 795 , 967 . other inhibitors include nucleic acids such as sirnas that are specific for inhibiting tnf expression . in addition to soluble receptors for lt , a wide variety of inhibitors or antagonists of lt are contemplated in this patent for reducing liver injury induced by various agents , including cd137 . inhibitors of lt include soluble forms of the ltβr extracellular domain . this can be achieved , for example , by fusing the extracellular domain of ltβr to an immunoglobulin constant heavy chain domain or to a human igg fc domain . other inhibitors include antibodies that bind to ltβr or lt . competitive antagonists of lt and nucleic acids inhibitors such as sirnas that are specific for inhibiting lt expression are also contemplated . ltβr is credited with developing lymphoid tissues but its role in other tissues has not been fully explored . ltβr &# 39 ; s high expression in liver and ltβr - deficient mice exhibiting reduced survival after partial hepatectomy suggest an active role of ltβr in promotion of hepatocyte proliferation or prevention of hepatic apoptosis . a question explored was whether ltβr signaling influences the liver injury induced by various tnfsf members . first , to investigate whether fas - induced hepatocyte injury is dependent upon the ltβr signaling , wt and ltβr - deficient mice were injected with anti - fas agonistic antibody ( fig1 a - 1g ). this treatment induces severe hepatitis in wild type ( wt ) mice by directly targeting hepatocytes , leading to super - acute liver failure . unexpectedly , almost all ltβr −/− mice survived , whereas wt mice died within 12 h after treatment ( fig1 a ). serum alanine aminotransferase ( alt ) levels , as an indicator of liver injury , were significantly reduced in ltβr mice at 3 h after anti - fas treatment ( fig1 b ). reduced liver injury in ltβr −/− mice was not due to an intrinsic resistance of ltβr −/− hepatocytes to fas - mediated apoptosis because ltβr −/− hepatocytes were sensitive to fas - mediated apoptosis in primary hepatocytes in vitro . to define which cells could influence fas - induced hepatitis , liver lymphoid cells were analyzed by flow cytometry . increased numbers of neutrophils were observed in wt mice injected with anti - fas antibody ( fig1 c ). however , neutrophil numbers were significantly reduced in ltβr −/− mice , which correlated with reduced myeloperoxidase ( mpo ) positive cells and liver injury compared to wt mice at 2 h after anti - fas antibody injection ( fig1 , c and d ). the reduced recruitment of neutrophils to the liver of ltβr −/− mice after fas stimulation was not attributable to defective neutrophil development since nave ltβr −/− mice contained normal neutrophil numbers in the liver and spleen ( fig1 c ). to define how ltβr controls neutrophil recruitment to the liver after fas stimulation , expression of chemokines cxcl2 , cxcl1 , and ccl3 that control neutrophil recruitment to the liver was analyzed ( fig1 e , fig1 f , fig1 g ). cxcl2 and cxcl1 are rapidly induced in the liver after fas stimulation and significantly contribute to neutrophil - mediated inflammation in the liver . reduced expression of cxcl2 ( mip - 2 ) and ccl3 was found in the liver of ltβr −/− mice , while expression of cxcl1 ( kc ) was not significantly different between wt and ltβr −/− mice ( fig1 e ). these results support that ltβr promotes pathogenic recruitment of neutrophils to the liver after fas stimulation by controlling expression of neutrophil attracting chemokines . fas and ltβr signaling are linked and likely coordinate since fas activation cannot fully induce liver injury in the absence of ltβr signaling . inhibition of ltβr signaling prevents hepatitis in a novel fas - independent liver injury model to define whether ltβr signaling is also involved in regulation of fas - independent liver injury agonistic anti - cd137 antibody , a strong t cell activator , was injected into fas −/− mice . cd137 ( 4 - 1bb ) is a member of the tnfr superfamily and primarily expressed on activated t cells . in wt mice this agonistic antibody induces subacute hepatitis within 7 days after injection that abates by day 14 ( fig2 , a and b ). this detailed analysis of intrahepatic lymphocytes in this severe hepatitis model parallels viral hepatitis in humans , in which there is heavy infiltration of cd8 + cells that secrete large amounts of ifn - γ , tnf , and chemokines are observed ( fig2 c , and fig3 , a and 3 c ). cd137 - induced hepatitis appears to be rich in cd8 + t cells , which represented the largest cell population in the liver of treated mice , whereas cd4 + t cells showed a lesser increase ( fig2 c ). however , unlike in cona - induced hepatitis model ( 3 ), cd4 cells do not appear to play an essential role , since fas −/− cd4 −/− double deficient mice were susceptible to cd137 - induced liver injury ( fig2 d and fig3 e ). in contrast , fas −/− cd8 −/− mice were essentially resistant to cd137 - induced liver injury ( fig2 e and fig3 f ). thus , activation of t cells by the cd137 costimulation pathway in fas - deficient mice results in development of a subacute cd8 - dependent hepatitis in mice . to test if inhibition of ltβr signaling could prevent hepatitis in the cd137 - induced hepatitis and thus uncover a potential treatment , the effect of soluble ltβr fusion protein ( ltβr - ig ) was tested . ltβr - ig given immediately before and again two days after anti - cd 137 administration resulted in markedly attenuated hepatitis with reduced serum alanine alt levels and minimal histological evidence of liver injury in fas −/− recipient mice ( fig2 , f - g ). ltβr - ig treatment also reduced the number of infiltrating lymphocytes in the liver compared to control rat - igg treated mice ( fig3 g ) inhibition of ltβr signaling by injection of ltβr - ig prevents the development of cd8 - dependent , fas - independent liver injury in cd137 stimulated mice . to test whether tnf is also involved in such t cell mediated hepatitis , the mice were treated with anti - cd137 as before , but 100 ug of tnfr - ig and ltβr - ig ( fig4 a ) was added . interestingly , both tnfr - ig and ltβr - ig can greatly reduce alt , supporting that tnf and lt are involved in liver injury . to test the role of light , another ligand for ltβr , hvem - ig that binds to light was used , but the blocking effect is limited ( fig4 b ). therefore , it is likely that both lt and tnf are required , but not light . ltβr signaling by membrane lt complex is essential for cona - induced liver injury to determine whether ltβr signaling is required for liver injury induced by cona , a strong and broader t cell activator that utilizes distinct ligands and cytokines that may each directly and indirectly cause liver injury , wt , ltβ −/− , ltβr −/− , and tnfr −/− mice were injected with a sublethal dose of cona ( fig5 and 5b ). quantitative serum alt at 24 hours after cona injection indicated significantly less hepatocyte injury in ltβ −/− , and ltβr −/− mice compared to wt mice ( fig5 a ). examination of liver tissue harvested 24 hours after cona administration revealed wt mice with geographic coagulative hepatocellular necrosis not seen in ltβ −/− and ltβr −/− mice ( fig5 b ). residual liver injury present in ltβ −/− compared to ltβr −/− mice ( fig5 a - b ) suggests that light signaling , the only other known ligand for ltβr , may additionally contribute to liver injury . this data demonstrates a distinct role of lt specifically in the pathogenesis of hepatitis that is not redundant . using a genetic knockout system , the inventor found that ltβ −/− mice , lacking surface lt , were protected from cona - induced liver injury even though unaltered light signaling persists ( fig5 a - 5b ). thus , this data demonstrates that ltβr deficiency confers resistance to cona - induced liver damage and that signaling by the ltα 1 β 2 through ltβr is necessary for developing cona - induced liver injury . therefore , blockade of lt or tnf by soluble receptors , or other inhibitors of lt or tnf expression or activity , will have broad impact on controlling liver injuries by various causes . inhibition of lt and ltβr signaling protects mice from cona - induced liver injury to determine whether inhibition of ltβr signaling could potentially protect mice from a broader form of liver injury induced by cona , wt mice were pretreated i . v . with 200 μg of control human igg or soluble ltβr fusion protein ( ltβr - ig ), which blocks ltβr signaling , 2 hours prior to cona injection ( fig6 a - 6d ). control human igg i . v . 2 hours prior to cona treatment in wt mice resulted in the expected marked alt elevations at 8 and 24 hours post - injection , whereas ltβr - ig resulted in substantially reduced alt levels ( fig6 b ). the ltβr - ig treatment markedly limited hepatocellular injury ( fig6 a - 6b ). to determine the influence on liver apoptosis , livers were harvested at 8 hours post cona administration and histological sections were assessed for tunel positive hepatocyte nuclei . wt mice treated with ltβr - ig had significantly fewer tunel positive nuclei ( fig6 c ). these data indicate that a pharmacologic blockade of ltβr reduces cona - induced hepatocyte injury , raising the possibility of treating broader forms of liver insults . since ltβr - ig blocks signaling through the ltβr from both light and the membrane lt complex ltα1β2 , the next test was whether specific inhibition of membrane lt reduces cona - induced hepatocyte injury ( fig6 d ). anti - ltβ antagonistic antibody given to wt mice 2 h prior to cona administration reduced liver injury relative to treatment with a control hamster antibody ( fig6 d ). together these data show that inhibition of ltβr signaling through ltα1β2 prevents cona - induced liver injury and illuminate a new direction for treatment of hepatitis through blockade of lt or tnf by soluble receptors , or other inhibitors of lt or tnf expression or activity . the ltβr pathway indeed plays a central and integral role in mediating the injury induced by different tnfsf members . this insight prompted the investigation of lt in t cell - mediated hepatitis , wherein multiple tnfsf pathways appear to coordinate the attack . chronic hepatitis c ( hcv ) viral infection induces t cell - mediated hepatitis , a chronic inflammation associated with an increased production of inflammatory cytokines leading to liver injury ( guidotti and chisari , 2006 ; mengshol et al ., 2007 ). studies in mice demonstrated that ectopic lt overexpression promotes similar chronic inflammation ( drayton et al ., 2006 ). however , whether lt has a significant role in hcv mediated liver injury remains largely unknown . to determine the role of lt in the pathogenesis of hcv - induced hepatitis , the inventor measured ltα mrna expression by real - time rt - pcr in liver tissue from human patients chronically infected with hepatitis c virus ( fig7 ). livers with chronic hcv infection showed significantly increased levels of ltα mrna expression when compared to uninfected controls ( fig7 ). these data suggest that lt may be involved in the pathogenesis of human hcv induced hepatitis and that blockade of lt by soluble receptors , or other inhibitors of lt expression or activity , will have broad impact on controlling hcv induced hepatitis . human samples . liver tissue samples were collected from chronically infected hcv patients at the time of liver transplantation according to approved procedures at johns hopkins school of medicine . active chronic hepatitis was confirmed by hematoxylin and eosin staining and ast levels (& gt ; 50 u / 1 ). all patients were diagnosed chronic hepatitis c virologically and serologically . all healthy donors were negative for hcv , hepatitis b virus ( hbv ), and human immunodeficiency virus ( hiv ). mice . c57bl / 6 male mice 8 - 10 weeks of age and fas −/− mice were purchased from the jackson laboratory ( jackson labs bar harbor , me ). fas −/− mice were crossed with cd4 −/− and cd8 −/− mice to generate fas −/− cd4 −/− and fas −/− cd8 −/− double deficient mice , respectively . ltβr −/− mice were kindly provided by k . pfeffer ( technical university of munich , germany ) lck - light transgenic mice ( light - tg ), ltβ −/− mice were genotyped as described ( wang et al ., 2001 ; tumanov et al ., 2002 ). all 8 - 10 weeks aged male mice were on c57bl / 6 background and were housed under specific pathogenfree conditions at the university of chicago . animal care and use were in accordance with institutional and nih protocols and guidelines , and all studies were approved by the animal care and use committee of the university of chicago . cona and antibody treatments . cona ( vector laboratories ) was administered i . v . to the mice at the indicated doses . sera were collected at the indicated times for measurement of cytokines and liver enzymes . inhibitors of lt and ltβr signaling : anti - ltβ blocking antibody ( bbf6 , 100 μg ) or soluble ltβr fused to human fc portion of igg ( ltβr - ig , 100 μg ) were injected i . v . 2 hours prior to cona injection . the ltβr - ig used in this study has been previously described . briefly , cdna encoding the extracellular domain of murine ltβr was fused with the fc portion of human igg , transfected into bhk / vp16 cell , and the supernatant collected . induction of hepatitis in fas −/− mice by administration of anti - cd137 . fas −/− mice were treated with 200 μg agonistic anti - cd137 ( 2a , rat igg2a ) by i . p . injection . the generation of 2a has been described previously ( wu et al ., 1999 ) and ascites were produced in scid mice and purified by passage over a protein g - coupled sepharose ® column . rat igg was purchased from sigma - aldrich and served as a control . rt - pcr analysis . total rna was extracted by rneasy ® mini kit from qiagen . for cdna synthesis , rnas were digested with dnase i and reverse transcribed using random primers with amv reverse transcriptase ( promega ) ( amv - rt ®). the concentration of the target gene was determined using the comparative c t ( threshold cycle number at a cross - point between amplification plot and threshold ) method and normalized to gapdh or hprt . cdna were amplified using taqman ® pcr master mix ( applied biosystems ) and run on abi 7900 cycler ( applied biosystems ). pcr primers and probes used : for human ltα : forward 5 ′- tggtgttggcctcacacct ( seq id no : 1 ), reverse 5 ′- ccaggagagaattgttgctc ( seq id no : 2 ), probe 5 ′- fam - ccacagcaccctcaaacctgc - tamra ( seq id no : 3 ); for human gapdh , forward 5 ′- gaaggtgaaggtcggagt ( seq id no : 4 ), reverse 5 ′- gaagatggtgatgggattt ( seq id no : 5 ), probe 5 ′- caagcttcccgttctcagc ( seq id no : 6 ); for murine mip - 2 ( cxcl2 ): sense 5 ′- ccaccaaccaccaggctacaggggc ( seq id no : 7 ), antisense 5 ′- aggctcctcctttccaggtcagttagc ( seq id no : 8 ); for murine hprt : forward : 5 ′- cagaggactagaacacctgc ( seq id no : 9 ), reverse : 5 ′- gctggtgaaaaggacctct ( seq id no : 10 ); for murine kc ( cxcl1 ): forward 5 ′- ccacccgctcgcttctc ( seq id no : 11 ), reverse 5 ′- cactgacagcgc agctcatt ( seq id no : 12 ); for murine mip - 1α ( ccl3 ): forward 5 ′- ccttgctgttcttctctgtaccatg ( seq id no : 13 ), reverse 5 ′- gcaatcagttccaggtgagtgatg ( seq id no : 14 ). histology and tunel labeling . tissues were fixed in 10 % buffered formalin and processed either for routine hemotoxylin and eosin staining or tunel and immunohistochemical studies . hematoxylin and eosin staining on sections of embedded tissues was performed according to standard procedure in the university of chicago pathology histology laboratory . tunel staining was performed on paraffin - embedded , formalin - fixed tissue using the apoptag ® plus peroxidase in situ apoptosis detection kit ( chemicon international ) according to the manufacturer &# 39 ; s directions . anti - gr - 1 ( rb6 - 8c5 clone ) ( bd biosciences ) and anti - myeloperoxidase ( mpo ) ( neomarkers ) antibody staining was performed on paraffin - embedded , formalin - fixed tissue . transaminase activity and cytokines analyses . blood was collected by retro - orbital puncture , following iacuc approved procedures . plasma alanine aminotransferase ( alt ) and aspartate aminotransferase ( ast ) activities were determined using a reflotron ® plus chemistry analyzer according to the manufacturer &# 39 ; s procedure ( roche diagnostics corp .). concentrations of cytokines in sera were determined by cytokine bead assay ( bd biosciences ) following the manufacturer &# 39 ; s recommendations . flow cytometry analysis . intrahepatic leukocytes were purified from the liver by pressing the liver through a steel sieve ( sigma , 190 μm ) into pbs , centrifuged at 800 g for 5 min with the resulting pellet suspended in a 35 % percoll ®- pbs - heparin ( 100 u / ml ) solution and centrifuged at 800 g for 20 min at room temperature . the pellet of mononuclear cells was cleared of rbc with a 5 - min osmotic lysis ( 0 . 15 m nh 4 cl , 1 mm khco 3 , 0 . 1 mm na 2 edta , ph 7 . 3 ) and washed twice in pbs . lymphocytes were stained with antibodies ( bd biosciences ) and analyzed by flow cytometry ( facscanto , bd biosciences ). statistical analysis . data are expressed as means ± sd . statistical significance was determined by a two - tailed student &# 39 ; s t - test (* p & lt ; 0 . 05 , ** p & lt ; 0 . 01 , *** p & lt ; 0 . 001 ), ns - not significant ( p & gt ; 0 . 05 ). abbreviations used : lt , lymphotoxin ; light , t - cell - restricted ligand , homologous to lymphotoxin , exhibits inducible expression , competes with herpesvirus glycoprotein d for herpesvirus entry mediator on t - cells ( tnfsf14 ); alt , alanine aminotransferase these publications are incorporated by reference to the extent they relate materials and methods disclosed herein . ajuebor , m . n ., c . m . hogaboam , t . le , a . e . proudfoot , and m . g . swain . 2004 . ccl3 / mip - 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