Patent Abstract:
the present invention provides compositions of matter comprising a polypeptide expressed from insect cells harboring a baculovirus vector the encodes the polypeptide , wherein the polypeptide comprises amino acid sequences derived from the p42 fragment of the plasmodium falciparum gp 195 protein or derivatives thereof . such compositions of matter find use for example for inducing the production of anti - p42 antibodies both in vivo and in vitro .

Detailed Description:
bvp42 antigen of plasmodium falciparum gp195 induces antibodies that strongly , if not substantially completely , inhibit parasite growth and forms the basis of a vaccine . the antibodies are extensively crossreactive with different parasite strains , and have been found to strongly or completely inhibit parasite growth of heterologous parasites to the same degree as homologous parasites . bvp42 , as described herein , is a variant of the natural p42 processing fragment of gp195 that has been recombinantly expressed in insect cells using a baculovirus expression vector . the term bvp42 is used herein to refer to the p42 amino acid sequence as characteristically produced in insect cells , in particular in sf9 insect cells . the amino acid sequence of p42 of different isolates is shown in fig6 i . e . amino acids nos . 1333 to 1726 in the fup isolate ( seq id no : 2 ) , 1308 to 1701 in the ( seq id no : 4 ); 1264 to 1640 in the wellcome isolate , and 1255 to 1631 in the k1 isolate ( seq id no : 3 ) ( the numbers refer to the amino acids of the precursor molecule , gp195 ). the term p42 refers to the corresponding sequences in other isolates as well . a preferred embodiment of the invention includes only the amino acids ala 1333 to ser 1705 of the fup isolate ( seq id no : 2 ) ( 33 ), or the corresponding amino acids of other isolates ( e . g . ala 1308 , to ser 1680 of the mad isolate ( seq id no : 4 ) ( 20 ), ala 1264 to ser 1619 of the wellcome isolate ( seq id no : 5 ) ( 19 ), ala 1255 to ser 1610 of the k1 isolate ( seq id no : 5 ) ( 20 )). ( the numbering of these amino acids also corresponds to that shown in fig6 .) the antigen of this embodiment deletes the anchor sequence at the c - terminus of p42 , allowing easier recovery of the product because it is secreted from the host cells . the amino acid sequences of isolates bearing the same designation may vary somewhat , as may the dna sequences coding for those isolates . similarly , the numberings of the amino acid and dna sequences in other publications may differ from the numberings shown herein . a particular amino acid and dna sequence of a fup isolate corresponding to amino acids ala 1333 to ser 1705 ( seq id no : 2 )( as shown in fig6 ) is described in the examples below and has the sequences shown in fig7 ( seq id no : 1 ). in another aspect of the invention , as noted above , we have found that when a p42 amino acid sequence is expressed in insect cells , a pure product can be obtained without degradation or cleavage if dna coding for a mad allele sequence is employed . an amino acid sequence of the p42 antigen is considered be of the mad allele if it corresponds to the parts of gp1 95 of the mad plasmodium falciparum isolate which are dimorphic as compared with the amino acid sequences of the wellcome and k1 isolates ( 13 ). for example , gp195 of the fup isolate is of the mad allele ( 23 , 33 ) ( fup isolate p42 amino acid sequence is as well ). the method for producing bvp42 according to this aspect of the invention involves infecting an insect cell host with a recombinant is baculovirus vector , that vector containing dna coding for a p42 amino acid sequence of surface protein gp195 of the plasmodium falciparum mad allele , operably linked to a baculovirus polyhedron promoter . production of bvp42 with the sequence of the mad allele , preferably of the fup isolate sequence , results in pure , undegraded antigen . a high level of inhibition of parasite growth is exhibited by anti - bvp42 sera . as noted above , strong inhibition of parasite growth by sera of monkeys vaccinated with mab 5 . 2 affinity purified parasite gp1 95 correlates with the ability to induce substantially complete protection against infection . without wishing to be bound by any theory of the invention , our results provide new insights into the interpretation of the vaccination study discussed above utilizing parasite gp195 that was affinity purified using mab 5 . 2 . as shown herein , utilization of this monoclonal antibody ( now found by us to be specific for a conformational epitope located within the p42 processing fragment ) for affinity purification of parasite gp195 results in an antigen preparation that contains the gp195 precursor but that is also highly enriched for several c - terminal processing fragments , including p42 . based on the analysis of the specificity of anti - parasite gp195 antibodies utilizing the bvp42 polypeptide , a majority of antibodies produced by immunization with mab 5 . 2 affinity purified parasite gp195 is specific for the c - terminal p42 processing fragment . the importance of p42 epitopes in immunity is consistent with the strong inhibition of parasite growth obtained with anti - bvp42 sera . there have been reports of several monoclonal antibodies specific for this region which inhibit in vitro parasite growth ( 21 , 22 ). thus , it is possible that the exceptional level of protection achieved in the previous vaccination study was due to the focusing of the immune response on c - terminal epitopes which appear to serve as targets of functional effects such as the direct inhibition of parasite growth . a study of the influence of mhc genes on immunological responsiveness to gp195 had found that a variety of congenic mouse strains were capable of producing antibodies against gp1 195 ( 23 ). we have detected bvp42 - specific antibodies in seven congenic mouse strains immunized with purified , parasite gp195 , indicating that individuals of many h - 2 haplotypes are capable of recognizing epitopes within the smaller , p42 region of gp195 . thus , bvp42 can likely be used as a vaccine antigen in hosts of diverse genetic makeup . techniques known to one skilled in the art for expressing foreign genes in insect host cells can be used to practice the invention . methodology for expressing polypeptides in insect cells is described , for example , in summers and smith ( 24 ), luckow ( 25 ), and in u . s . pat . no . 4 , 745 , 051 , all hereby incorporated by reference . the techniques are summarized below . to make bvp42 , or an effective immunogenic part thereof , a polyhedron shuttle vector is used to shuttle all or part of the p42 coding sequence into a nuclear polyhedrosis virus . this vector contains the promotor of the polyhedron gene of a nuclear polyhedrosis virus and an available cloning site for the insertion of a selected gene such that the selected gene is under transcriptional control of the polyhedron promotor . thus , the shuttle vector is engineered to contain the p42 sequence operably linked to a baculovirus polyhedrin promoter . for example , the p42 sequence can be inserted into a commonly used polyhedron transfer vector , such as pac373 , to produce a recombinant shuttle vector for introducing foreign genes into a nuclear polyhedrosis viruses , such as polyhedron californica nuclear polyhedrosis virus ( acmnpv ), resulting in a recombinant viral expression vector capable of expressing the gene encoding for p42 in a host insect cell . pac373 contains a deletion of the sequence between − 8 ( 8 bases upstream from the polyhedron atg and approximately 40 bases downstream from the polyhedron transcriptional start site ) and the natural bamh1 sites at nucleotide + 171 ( 26 ) resulting in a construct which can be used to express full - length gene which contains an internal atg ( n - formyl methionine initiation ). any p42 coding dna can be used to make the expression vector . while natural dna sequences of various plasmodium falciparum isolates ( seq id no : 1 ) are described in the examples , it is within ordinary skill in the art to vary those sequences . thus , non - natural dna sequences , different from the particular ones listed herein , can be used effectively in practicing the invention . similarly , non - natural amino acid sequences can be coded for by the dna used in the transfer vector in order to obtain functional products that obtain the advantages of the invention . the c - terminus of bvp42 is preferably truncated , as discussed above , to remove the hydrophobic tail sequence ( i . e ., membrane anchor ) to allow for protein secretion . for example , the entire anchor sequence e . g . amino acids 1708 - 1725 of the fup isolate ( seq id no : 2 ) and corresponding amino acids of other isolates , can be deleted . an embodiment discussed in the examples below deletes an additional two amino acids at the n - terminus of the anchor sequence . thus it is within the scope of the invention to delete portions of the p42 amino acid sequence which do not affect the beneficial result obtained with the bvp42 products exemplified herein . transfer of the hybrid dna to an expression vector is accomplished by transfection of the host insect cell , e . g . sf , with a mixture of both the recombinant transfer plasmid dna and wild type nuclear polyhedrosis virus ( mnpv ). the transfected plasmid dna recombines with the homologous sequences in the wild - type baculovirus genome to produce a viral genome that carries an integrated copy of the foreign gene . the recombinant expression vector , comprising the hybrid polyhedron - p42 gene incorporated in the mnpv genome is then selected from the mixture of nonrecombinant and recombinant baculoviruses . for example , the supernatant containing the mixture of wild - type and recombinant budded viruses is collected , clarified by centrifugation and used for subsequent plaque assays . the preferred means of selection is by visual screening for the absence of viral occlusion bodies by plaque hybridization with dna coding for p42 , such as described in kiefer et al . ( 27 ). expression of the p42 gene is accomplished by infecting susceptible host insect cells , such as sf cells , with the recombinant baculovirus p42 - expression vector in an appropriate medium for growth . propagation of the baculovirus p42 - expression vector is achieved in the insect cells through replication and assembly of infectious virus particles . various nuclear polyhedrosis viruses can be employed in making expression vectors for use in the invention . suitable viruses include , but are not limited to , polyhedron californica nuclear polyhedrosis virus ( acmnpv ), spodoptera frugiperda nuclear polyhedrosis virus , choristoneura fumiferana nuclear polyhedrosis virus , spodoptera littoralis nuclear polyhedrosis virus , or trichoplusia ni nuclear polyhedrosis virus , all known in the art and commonly available . suitable insect host cells for use in the invention include , but are not limited to , sf9 ( spodoptera frugiperda ), spodoptera exiaua , choristoneura fumiferana , trichoplusia ni , and spodoptera littoralis . the polypeptide may be produced as either a fusion product , such as a heterologous protein containing part baculovirus amino acid sequence fused to plasmodium falciparum sequence , or the product may merely contain a plasmodium falciparum sequence . methods of making both types of proteins are well known to one skilled in the art . dna encoding a leader sequence can be included in the expression vector to facilitate sorting through the endoplasmic reticulum and proper folding of the polypeptide product . for example , flg5 cdna encoding a leader sequence can be inserted 5 ′ to the p42 coding region using conventional techniques . the flg5 leader is described in kiefer et al . ( 27 ). it is also disclosed in the genebank and embl data bases ( accession no . m60485 ). in addition , the flg5 dna sequence ( seq id no : 1 ) used in a construct described in the examples below is shown in fig7 . the final purified product according to the invention may include a leader sequence , or the sequence may be cleaved during expression . it is believed that the flg5 leader is cleaved during expression . the many variations in techniques for expressing and isolating bvp42 will be apparent to one skilled in the art . the bvp42 product can be conventionally purified , such as , in a part , by affinity chromatography with a monoclonal antibody specific for natural p42 . mab 5 . 2 is one such antibody . the bvp42 obtained is a variant of naturally occurring p42 that results from characteristic post - translational processing occurring in insect cells , especially sf9 cells . potential sites for post - translational modifications are shown in fig6 . bvp42 has been found to be highly immunogenic in rabbits . high antibody titers against the immunogen can be obtained which meet or exceed titers of animals immunized with purified parasite gp195 . elisa titers were found similar in assays utilizing plates coated with either purified , parasite gp195 or bvp42 . more importantly , high titers were obtained when anti - bvp42 antibodies were reacted with purified , parasite gp195 in an elisa and in an indirect immunofluorescence assay with schizonts and merozoites . ifa titers obtained after the fourth immunization with bvp42 reached levels exceeding those obtained by immunization with purified , parasite gp195 . yeast produced p42 ( yp42 ) consisting of the same p42 sequence ( amino acids nos . 1333 to 1705 in the fup isolate , ( seq id no : 2 ) was found to be less immunogenic than bvp42 , inducing lower antibody titers against the immunogen . in addition , the cross - reactivity of anti - yp42 antibodies with parasite gp195 in the elisa was much lower than cross - reactivity of anti bvp42 antibodies . yp42 also induced much lower ifa titers than bvp42 , and statistically insignificant levels of parasite inhibition . immunoblotting studies demonstrated that most of the anti - bvp42 and anti - parasite gp195 antibodies produced are specific for disulfide - dependent , conformational epitopes present on the same set of gp195 processing fragments . an adjuvant should be included with the bvp42 in the vaccine of the invention in order to enhance the immune response . such adjuvants include freunds complete adjuvant , b30 - mdp , la - 15 - ph , ( 28 , 29 , 32 ) saponin , aluminum hydroxide , or other available adjuvants or combinations of adjuvants . freund &# 39 ; s complete adjuvant is not generally used clinically for human vaccines . a pharmaceutically acceptable carrier should also be included in the vaccine . such carriers are well known to one skilled in the art and include , for example , liposomes or saline solution . the vaccine preferably contains from about 0 . 2 to 5 mg in a one ml dose of bvp42 . the vaccine can be administered in one or more doses , the amount administered being adjusted to correspond with the number of innoculations , either together or over a period of time . administration can be carried out conventionally , preferably parenterally . the present invention is further described in the examples below . the examples are for illustration purposes , and are not intended to limit the scope of the invention . the uganda palo alto ( fup ) plasmodium falciparum p42 coding region from ala 1333 to ser 1705 was cloned into a polyhedron californica nuclear polyhedrosis virus ( acmnpv ) polyhedrin promoter regulated expression system ( 26 ). fig1 shows , schematically , the sequences that were cloned into a bvp42 encoding shuttle vector . the coding sequence , shown at top , included the flg5 leader ( flg l , solid box ) fused to the fup isolate p42 coding region from ala 1333 to ser 1705 . the bamh1fragment finally obtained was cloned into a acmnpv transfer plasmid , shown at the bottom of the figure . the leader sequence for flg5 has been shown to direct secretion of the mature flg5 protein ( 27 ). when fused to p42 coding dna in the baculovirus expression system , the mature protein is also found to be secreted from the insect cells . “ rv ” is an ecorv restriction site . the construct was made as follows . oligomers encoding the flg5 leader ( 27 ), 5 ′ portion of fup isolate p42 from ala 1333 to the hindiii site at amino acid arg 1362 and bamh1 sites were made on an automated dna synthesizer ( applied biosystems , foster city , calif . ), kinased , annealed , and ligated . the overlapping oligomers consisted of six 43 - mers , two 46 - mers and two 19 - mers . after digestion with bamh1 , the ligation product was ligated into bamh1 - digested and phosphatased pab114 , a hindiii / sa1 deletion of pbr322 containing a bamh1 linker ( 30 ). the hindiii / sal1 fragment of p42 from a pab125 adh 2 - gapdh α - factor construct ( 32 ), which includes an in - frame stop codon following ser 1705 , was ligated in - frame into this pab114 vector to yield a flg5 leader fused to full - length p42 flanked by unique bamh1 sites . the bamh1 fragment was subsequently cloned into the bamh1 site of the acmnpv transfer plasmid pac373 ( 26 ). the recombinant shuttle vector obtained was then used to transfer the coding sequence into acmnpv by homologous recombination through transfection of sf9 cells ( 24 ). recombinant viruses were initially identified visually by occlusion negative phenotype and plaque hybridization with the bamh1 fragment labeled by the oligomer primed extension method ( 31 ). the recombinant acmnpv expression vector obtained , flg5lfup42acnpv , was deposited with the american type culture collection , 12301 parklawn drive , rockville , md . 20852 , u . s . a . on jan . 28 , 1992 and has been assigned accession number vr2354 . infection of sf9 cells was carried out conventionally per summers and smith ( 24 ). protein expression was initially monitored in roller bottles . production was then scaled up to 0 . 5 liter batches in 2 . 8 liter fernbach shaker flasks . culture supernatants were concentrated 10 to 50 - fold by tangential flow ultrafiltration ( amicon ). recombinant bvp42 was purified from the concentrated supernatant by the affinity chromatography technique utilized to purify parasite gp195 as described by siddiqui et al . ( 7 ) ( summarized below in example iii ). fig2 a shows silver stains ( lane 1 ) and immunoblots ( lane 2 ) of the affinity chromatography purified bvp42 . the silver stained antigens were electrophoresed in sds polyacrylamide gels . the immunoblots were reacted with a rabbit anti - parasite gp195 serum pool . a single band migrating at approximately 44 kda was observed in the silver stain . in the immunoblots , the purified bvp42 displayed major immunoreactive species at the positions of the major protein bands , accompanied by minor reactivities with proteins of higher and lower molecular weight . the dna sequence of the bvp42 construct from bamhi to sali is shown in fig7 the numbering starting with the first nucleotide of bamhi and ending with the last nucleotide of sali . a restriction map is shown schematically at the top of the figure and the same sites are shown adjacent to the corresponding sequences below . a yeast p42 construct used in the examples was expressed in the alcohol dehydrogenase 2 / glyceraldehyde - 3 - phosphate dehydrogenase ( adh 2 - gapdh ) regulated expression system of saccharomyces cerevisiae and has been described fully in hui et al . 1991 , incorporated by reference ( 32 ). the p42 construct was based on the gp195 coding region from ala 1333 to ser 1705 of the fup isolate ( seq id no : 2 ) ( 33 ). the yeast p42 polypeptide ( yp42 ) was purified using an affinity chromatography technique described by siddiqui et al . 1987 for the purification of parasite gp195 ( 7 ) ( summarized below ), except that polyclonal rabbit anti - gp195 igg was used for antigen purification instead of anti - gp195 monoclonal antibody 5 . 2 , because the yp42 protein could not be recovered in significant amounts using this monoclonal antibody . fig2 b shows silver stains ( lane 1 ) and immunoblots ( lane 2 ) of the affinity chromatography purified yp42 . the silver stained antigens were electrophoresed in sds polyacrylamide gels . the immunoblots were reacted with a rabbit anti - parasite gp195 serum pool . in the silver stain , a major species corresponding to yp42 migrated as a 44 kda molecule , although other minor bands were also present in varying amounts . in the immunoblots , the purified yp42 displayed major immunoreactive species at the positions of the major protein bands , accompanied by minor reactivities with proteins of higher and lower molecular weight . isolation of purified , parasite gp195 with selected fragments that can induce substantially complete protection against plasmodiusn falciparum challenge a mixture of gp195 protein and certain of its processing fragments was obtained from in vitro cultured parasites ( p . falciparum uganda palo alto strain ) using monoclonal - antibody affinity chromatography procedures employing nab 5 . 2 ( 7 ); i . e . the mixture is enriched in the epitope for which mab 5 . 2 is specific . importantly , this mixture has been previously shown to be capable of inducing substantially complete protection against a homologous challenge of plasmodium falciparum in aotus monkeys ( 7 ). references in the examples below to “ gp195 ” refer to the mab 5 . 2 - purified mixture of gp195 enriched with certain of its processing fragments . in summary , saponin - lysed parasites were extracted with 1 % np - 40 and the lysate was clarified by ultracentrifugation . the extracts were passed through a protein g sepharose column covalently conjugated with gp195 - specific monoclonal antibody mab 5 . 2 ( described in u . s . pat . no . 4 , 897 , 354 , incorporated by reference herein , and deposited with the american type culture collection , 12301 parklawn dr ., rockville , md . 20852 , u . s . a . on jul . 17 , 1986 under accession number hb 9148 ). mab 5 . 2 is specific for an antigenic determinant contained in p42 , as demonstrated below . after extensive washing to remove non - specifically bound material , specifically - bound proteins were eluted with 0 . 1 m glycine ( ph 2 . 5 ) and neutralized with 1 m tris - hcl ( ph 8 . 0 ). the purity of the isolated gp1 95 was examined by sds - page followed by silver staining . substantially complete parasite inhibition was found obtainable using bvp42 as an immunogen in in vitro parasite growth inhibition assays . this level of inhibition has only observed by us to be induced by the mab 5 . 2 affinity - purified mixture of gp195 and fragments ( 8 ). in vitro parasite growth inhibition assays were performed by culturing parasites in the presence of immune rabbit serum using established methods ( 8 ). briefly , parasites were cultured in the presence of 15 % preimmune serum or immune rabbit serum obtained 14 , 21 , 28 , and 35 days after the fourth immunization with bvp42 in complete freund &# 39 ; s adjuvant ( indicated as 4d14 , 4d21 , 4d28 and 4d35 in table i below ). the starting parasitemia ( s ) in each of the experiments was 0 . 2 %. growth inhibition was calculated according to the following equation : %   inhibition = ( p - s ) - ( t - s ) ( p - s ) × 100  % , where p =  %   parasitemia   of   cultures   containing   15  %  preimmune   serum   at   72   hours , t =  %   parasitemia   of   cultures   containing   15  %  immune   serum   at   72   hours , and s = starting   %   parasitemia   of   cultures   at   0   hours quaternary sera were used in inhibition assays . the corresponding pre - immune serum of each animal was used as a control . parasite cultures were synchronized by sorbitol lysis ( 34 ) to select for late trophozoite and schizont stages . infected erythrocytes were adjusted to an initial parasitemia of approximately 0 . 2 % and a hematocrit of 0 . 8 % with fresh erythrocytes . rabbit preimmune or immune serum was added to a final concentration of 15 %, and 200 ul of the culture suspension was added in duplicate wells to a 96 - well microtiter plate . cultures were incubated at 37 ° c . for 72 hours , and the parasitemia was determined by microscopy . the experiment was repeated three times for each of the rabbits used ( rabbit given identification nos . 131 and 132 ). results are shown in table i . quaternary sera of rabbits immunized with yp42 had no significant effect on in vitro parasite growth ( data not shown ). in contrast , significant inhibition was obtained with quaternary sera of several bleeding dates from both rabbits immunized with bvp42 ( table i ). sera obtained from later bleedings of rabbit 132 ( 4d21 , 4d28 , 4d35 ) nearly completely inhibited parasite growth ; similar levels of inhibition have been observed previously only with antisera against mab 5 . 2 purified , parasite gp195 ( 8 ). a . elisa titers of rabbits immunized with purified parasite gp195 against gp195 , bvp42 and yeast p42 . rabbits were immunized with bvp42 , yp42 or enriched gp195 mixture ( i . e . enriched for gp195 and c - terminal containing processing fragments through mab 5 . 2 affinity purification ) to determine the immunogenicity of the recombinant polypeptides and the cross - reactivity of anti - recombinant p 42 antibodies with native gp195 . rabbits given identification nos . 103 , 104 , 106 and 115 were immunized with purified , parasite gp195 emulsified in complete freund &# 39 ; s adjuvant as described ( 8 ). rabbits 131 and 132 were immunized intramuscularly four times at 21 - day intervals with 50 ug of purified bvp42 in complete freund &# 39 ; s adjuvant . rabbits 93 and 96 were immunized intramuscularly five times at 21 - day intervals with 50 ug of purified yp42 in complete freund &# 39 ; s adjuvant . the amount of mycobacteria was reduced to one half of the first dose for the second immunization and one fourth of the first dose for subsequent immunizations , with the volume being replaced with incomplete freund &# 39 ; s adjuvant . rabbits were bled before immunization and weekly after each immunization . serum antibodies produced against enriched gp195 mixture , bvp42 , or yp42 were assayed by an enzyme - linked immunosorbent assay ( 23 , 33 ) using the following technique . vinyl plates were coated with purified , parasite gp195 and fragments ( 0 . 08 ug / ml ), recombinant bvp42 ( 0 . 08 ug / ml ), or recombinant yp42 ( 0 . 2 ug / ml ) and washed and blocked with 1 % bovine serum albumin in borate buffered saline ( bbs : 167 mm borate / 134 mm nacl , ph 8 . 0 ). rabbit and mouse sera were serially diluted in 1 % bsa / bbs , and human sera were serially diluted in phosphate - buffered saline ( pbs : 150 mm sodium phosphate , 500 mm nacl , ph 7 . 4 ) containing 0 . 05 % tween 20 , 1 . 5 % powdered milk , 0 . 05 % bsa and 0 . 05 % thimerosal . diluted sera were added to antigen - coated wells and incubated for 1 hr at room temperature . plates were washed with bbs containing 0 . 5 m nacl ( hsbbs ) ( rabbit and mouse sera ) or pbs with 0 . 05 % tween 20 ( human sera ), and an appropriate dilution of peroxidase - conjugated species - specific anti - igg ( heavy and light chain specific ) was added and incubated for 1 hr ( anti - rabbit and anti - mouse igg ) or 2 hrs ( anti - human igg ) at room temperature . plates were washed in hsbbs and finally in bbs . one hundred microliters of peroxidase substrate solution [ h 2 o 2 and 2 , 2 40 - azino - bis ( 3 - ethylbenzothiazoline - 6 - sulfonate )] were added to each well , and the absorbance value at 410 nm was determined with a dynatech 605 elisa reader . the endpoint of elisa titers for rabbit sera was designated to be the serum dilution producing an absorbance value of 0 . 2 , which corresponded to twice the background o . d . reading . the elisa endpoint titers for human sera was designated to be the serum dilution producing an absorbance value greater than 0 . 056 , which was two standard deviations above the average reading for normal human sera . a comparison of the reactivity of the polyclonal antisera from rabbits immunized with the purified , enriched parasite gp195 , bvp42 , and yp42 in an elisa is shown in table ii . the highest elisa titers were obtained against parasite gp195 , the immunogen , with lower titers against bvp42 , and the lowest titers against yp42 . these results are consistent with the expectation that antibodies are induced by gp195 outside of the p42 fragment , leading to a higher titer against the gp195 preparation than against bvp42 . b . elisa and ifa titers of rabbits immunized with bvp42 and yeast p42 against parasite gp195 , bvp42 and yp42 . additional rabbits were immunized with purified bvp42 ( nos . 131 , 132 ) or purified yp42 ( nos . 93 , 96 ) to determine the immunogenicity of the recombinant polypeptides and the cross - reactivity of anti - recombinant p42 antibodies with native gp195 . rabbit sera were tested by elisa , parasite indirect immunofluorescence ( ifa ), and immunoblotting with parasite gp195 . the ifa procedure used was as follows . assays were performed on acetone - fixed thin blood smears of schizonts and merozoites as described ( 35 ). endpoint ifa titers corresponded to the final serum dilution producing parasite immunofluorescence above background levels observed using preimmune rabbit sera . sera were obtained on the day indicated ( day 14 , 21 , 28 or 35 , indicated as 4d14 , etc . in table iii below ) after four immunizations of rabbits with 50 ug bvp42 or 50 ug yp42 . serial dilutions of sera were titered for reactivity with plates coated with recombinant p42 ( rp42 ) or parasite gp195 . “ rp42 ” corresponds to bvp42 for rabbit anti - bvp42 sera and yp42 for rabbit anti - yp42 sera . the method of determining the elisa endpoint titer for rabbit sera was as discussed above . endpoint ifa titers correspond to the final serum dilution producing parasite immunofluorescence above preimmune serum backgrounds . bvp42 proved to be highly immunogenic as shown in table iii , inducing antibody titers comparable to those of rabbits immunized with purified , parasite gp195 ( table ii ). as shown in table iii , elisa titers were similar in assays utilizing plates coated with either purified , parasite gp195 or bvp42 . very high elisa titers were obtained late in the quaternary response ( days 28 and 35 ). yp42 was less immunogenic than bvp42 , inducing lower antibody titers against the immunogen . in addition , the cross - reactivity of anti - yp42 antibodies with parasite gp195 in the elisa was much lower than the cross - reactivity of anti - bvp42 antibodies . typical merozoite surface staining patterns were observed by ifa for the bvp42 antisera ( data not shown ), and ifa titers obtained after the fourth immunization ( table iii ) reached levels exceeding those obtained by immunization with purified , parasite gp1 95 ( data not shown ). yp42 induced much lower ifa titers . in order to obtain an estimate of the degree of cross - reactivity of anti - parasite gp195 ( enriched mixture ) antibodies with bvp42 and yp42 , we performed an elisa inhibition assay utilizing the various antigens as inhibitors . the elisa inhibition assay measures the reduction in reactivity of anti - parasite gp195 sera with parasite gp195 antigen in the presence of various concentrations of soluble parasite gp195 or recombinant p42 . the elisa inhibition assay was performed by diluting rabbit antisera to a point on the descending portion of the elisa titration curve . the diluted sera ( rabbit anti - parasite gp195 sera 103 , 104 , 106 , and 115 ) were mixed with various concentrations of inhibitor ( gp1 95 , bvp42 or yp42 ), incubated for 1 hour , and added to purified , parasite gp195 - coated plates . subsequent steps in the elisa inhibition assay were identical to the standard elisa assay described in section a above . the results are shown in fig4 . soluble parasite gp195 completely inhibited the binding of anti - gp195 sera in this assay . a high level of inhibition ( 82 - 92 %) was also obtained with soluble bvp42 . much lower levels of inhibition were seen with soluble yp42 ( 28 - 47 %). the average antigen concentration required to obtain 50 % inhibition in the elisa was similar for parasite gp195 ( 0 . 03 ug / ml ) and bvp42 ( 0 . 04 ug / ml ), while & gt ; 5 ug / ml yp42 was required for 50 % inhibition . these results suggest that a majority of antibodies against parasite gp195 also recognize bvp42 , i . e . purified , parasite gp195 and bvp42 are highly cross - reactive . the recognition of gp195 ( without mab 5 . 2 affinity purified fragments ) and related recombinant polypeptides by serum antibodies of individuals from a malaria - endemic area of the philippines have been analyzed by kramer and oberst . several individuals from this study were examined for antibody reactivity with parasite gp195 ( mab 5 . 2 affinity purified ), bvp42 and yp42 . the results are shown in table iv . serial serum dilutions were tested for reactivity with the plates coated with parasite gp195 , bvp42 and yp42 . endpoint titers were designated as the serum dilution producing an o . d .& gt ; 0 . 056 , which was two standard deviations above the average reading for normal human sera . there was an excellent correlation between elisa titers obtained with parasite gp195 and bvp42 ( pearson &# 39 ; s correlation coefficient r = 0 . 96 , p & lt ;. 001 ). a lower correlation was obtained for gp195 and yp42 elisa titers ( pearson &# 39 ; s correlation coefficient r = 0 . 86 , p & lt ;. 01 ). thus , bvp42 cross reacts with serum antibodies of humans from the malaria endemic area of the philippines . e . recognition of conformational determinants by antibodies of rabbits immunized with parasite gp195 and recombinant p42 and by a gp195 specific monoclonal antibody ( mab 5 . 2 ). fig3 panels a and b show immunoblots of purified parasite gp195 electrophoresed under nonreducing ( panel a ) or reducing ( panel b ) conditions in a 11 . 5 % sds - polyacrylamide gel . immunoblots were reacted with the following antibody preparations : lane 1 , anti - gp195 monoclonal antibody 5 . 2 ; lane 2 , rabbit anti - parasite gp195 ; lane 3 , rabbit anti - bvp42 (# 131 ); lane 4 , rabbit anti - bvp42 (# 32 ); lane 5 , rabbit anti - yp42 (# 93 ); lane 6 , rabbit anti - yp42 (# 96 ). panel c : immunoblots of bvp42 ( lane 1 , non - reduced ; lane 2 , reduced ) and yp42 ( lane 3 , non - reduced ; lane 4 , reduced ) reacted with mab 5 . 2 . the procedure for immunoblotting was as follows . purified gp195 and bvp42 polypeptides were dissolved in laemmli &# 39 ; s buffer with or without 2 - mercaptoethanol as a reducing agent and separated on nadodso 4 polyacrylamide gels ( 36 ). the separated proteins were electrophoretically transferred to nitrocellulose ( 37 ) and reacted with rabbit and mouse antisera as described by chang et al ., 1989 ( 23 ). fig3 a & amp ; b , lane 1 , show that mab 5 . 2 is specific for a conformational determinant of the p42 fragment of gp195 since reactivity with this antibody was diminished by reduction of the parasite antigen . fig3 ( fig3 b lanes 2 , 3 and 4 ) also demonstrates that reactivity with the p42 processing fragments of both anti - gp195 sera and anti - bvp42 sera was markedly decreased when parasite gp195 was electrophoresed under reducing conditions . this indicates that both these antisera primarily recognize disulfide , conformational determinants of parasite gp195 . in contrast , reactivity of anti - yp42 sera , as shown in fig3 b ( lanes 5 and 6 ) was slightly enhanced for reduced gp195 , and the reduced 19 kda processing fragment was recognized by these sera . thus , the anti - yp42 appears to recognize gp195 epitopes that are not disulfide - dependent . the strong reactivity of bvp42 with monoclonal antibody 5 . 2 , as shown in fig3 c , lane 1 , and the loss of this reactivity by reduction of bvp42 , as shown in fig3 c , lane 2 , indicates that the conformation of bvp42 closely resembles that of the native protein . it has previously been shown that responsiveness to purified , parasite gp195 is present in mice of diverse major histocompatibility complex makeup ( 23 ). we determined whether the bvp42 antigen would be similarly recognized by anti - gp195 antibodies from a panel of congenic mouse strains . sera of seven congenic mouse strains possessing different h - 2 haplotypes on the b10 background and immunized with purified , parasite gp195 were tested for reactivity with the bvp42 antigen ( fig5 ). congenic mice of the following strain designations differing in h - 2 haplotype but sharing the c57bl / 10 genetic background were immunized : c57bl / 10 snj , b10 . a / sgsnj , b10 . d2 / nsnj , b10 . m / sn , b10 . wb ( 69ns ), b10 . br / sgsnj , and b10 . pl ( jackson laboratories , bar harbor , me .). five mice per group were immunized intraperitoneally four times at 2 - week intervals with 5 μg of purified , parasite gp195 emulsified in complete freund &# 39 ; s adjuvant . mice were bled before immunization , on days 7 and 10 after the first immunization and on days 5 , 7 , and 14 after subsequent immunizations . all seven strains produced anti - gp195 antibodies recognizing epitopes of bvp42 with some variation in titer among strains . thus , similar to parasite gp195 , individuals of diverse mhc haplotypes are capable of producing antibodies recognizing bvp42 . since the gp42 processing fragment contains both conserved and allelic determinants , the reactivity of anti - bv - p42 antibodies with homologous vs . heterologous gp195 antigens was characterized . four parasite isolates , fup , fvo , hond - 1 and pf857 were used and southern blot analyses with allele specific oligonucleotide probes showed that fup and pf857 belong to the mad allele , and fvo and hond - 1 belong to the k1 allele . native gp195 antigens were purified by affinity chromatography from fup and fvo parasites . in elisas , identical titers and binding curves were obtained with anti - bvp42 antibodies using either fup ( homologous ) or fvo ( heterologous ) gp195 as antigens . similar results were obtained in indirect immunofluorescent assays with fvo and fup merozoites . more importantly , anti - bvp42 antibodies strongly or completely inhibited the in vitro parasite growth of the heterologous parasites ( fvo and hond - 1 ) to the same degree as with the homologous parasites ( fup and pf857 ). all patents , publications and patent applications referred to herein are hereby incorporated by reference . 1 . holder , a . a . and r . r . freeman . 1981 . immunization against blood - 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