Patent Abstract:
the invention relates to a functional flour - based food composition , in particular baked products , and a method for the preparation thereof . specific substances of vegetable origin principally containing policosanols , isoflavones and vegetable statins are added to the flour , or to the dough for preparing the aforesaid baked products . further components can be added to the preparation in order to enhance the effectiveness and sensory qualities thereof . the functional food composition of the invention favors the re - balancing of cholesterol and triglyceride values .

Detailed Description:
the principal object of the present invention is to provide a flour - based food composition comprising vegetable substances that are useful for stabilizing cholesterol and triglyceride levels . a further object of the present invention is to provide a method for preparing baked food products which are capable of exerting an action of stabilizing cholesterol and triglyceride levels . the flour used as the base of the food composition of the invention is cereal flour . preferably , a soft wheat flour is used . the term flour is meant to refer both to pure wheat flour , corresponding to milled wheat , and the wheat flour - based food composition comprising other types of edible flours and / or chemical additives , also called “ improvers ”, which are mainly useful for improving the bread - making process . among the various substances that can be added we find , for example , malt flour , soy flour , l - ascorbic acid , enzymes , emulsifiers such as esters of mono - and diglycerides , dextrose , sucrose , alpha - and beta - amylase , stabilizers such as guar seed flour , and still others . the food composition of the present invention can consist exclusively of flour and the specific vegetable substances that exert the hypocholesterolemic action , or else it can be a dough for preparing baked food products also comprising water , salt , leavening , and still other substances . in addition to the phytosterols , isoflavones and policosanols mentioned above , further substances derived from plant species can be advantageously added to the functional food composition of the present invention . plant sterols , or phytosterols , are molecules of a sterolic nature present in plants ; they in fact make up part of plant cell membranes . about 40 phytosterols are known ; the phytosterols most present in the diet are betalo sitosterol ( 50 %), campesterol ( 33 %) and stigmasterol ( 4 %). as may be noted from the percentages , the other phytosterols are present in very modest amounts . beta - sitosterol differs from cholesterol because of the ethyl group at position 24 of the side chain . it is by now well - established that phytosterols are effective against high blood cholesterol . their mechanism of action is very simple : phytosterols decrease the cholesterol levels in blood by reducing intestinal cholesterol absorption . this mechanism is based on the substitution of cholesterol inside micelles , which are veritable means of transport for cholesterol . given that micelles have a limited capacity to incorporate phytosterols , the concentration of cholesterol in the same is reduced and , accordingly , less is absorbed through the intestinal membrane . the cholesterol not incorporated into the micelles forms co - crystals with the phytosterols and together they are eliminated through faeces . phytosterols , unlike cholesterol , are not capable of producing atherosclerotic plaques . in the diet , they are found especially in vegetable oils , nuts and some seeds and legumes . one particularly interesting source of phytosterols is a plant of the family cucurbitaceae , whose scientific is cyclanthera pedata . the clinical studies conducted demonstrate that cyclanthera pedata helps to reduce blood cholesterol levels . through its use it is possible to achieve an 18 % reduction in total cholesterol levels and a 23 % reduction in bad cholesterol or ldl , with the additional advantage of raising the level of good cholesterol or hdl by up to 42 %. in the composition of caigua ( one of the common names of cyclanthera pedata ) there are many elements which explain these anticholesterol effects . the best known are phytosterols such as sitosterol - 3 - 5 beta - d - glycoside . saponins , or saponin glycosides , are complex molecules characterized by an aglycone structure of a steroidic or triterpenic nature . they are responsible for a cholesterol lowering activity thanks to which total cholesterol and ldl cholesterol are significantly reduced . they are found , lo for example , in glycine max and medicago sativa extracts . folic acid , or pteroyl ( mono ) glutamic acid or vitamin b9 , exerts an action of limiting homocysteine , which is a sulphur - containing amino acid that forms following the enzymatic transformation of methionine , another sulphurated amino acid present in proteic foods ( dairy products , meat , legumes , eggs ) and which can prove even more harmful than cholesterol . folic acid is contained , for example , in medicago sativa extracts . coenzyme q10 , also called ubiquinone , or vitamin q , is an organic molecule , and more precisely a benzoquinone with very long isoprene side chain . this coenzyme , ubiquitous in biological systems , displays a structure similar to that of vitamin k and vitamin e . it is found in abundance in soybeans , cereals , walnuts and grapes . in organisms , it participates in redox reactions . it possesses a strong scavenger action and for this reason protects cellular structures against free radicals . it performs its action synergistically with vitamin e , protected in turn by coenzyme q10 , which assures the bond thereof with octacosanol ; the latter , in turn , is also bonded to b group vitamins and minerals . this coenzyme is in fact a water - insoluble lipophilic compound with adjuvant action in the transport of electrons and in mitochondrial energy production . the intake of coenzyme q10 can exert cardioprotective , cytoprotective and neuroprotective effects ; it also performs an action of inhibiting the oxidation of ldl cholesterol , which is believed to be the largest pathogenetic component of atherosclerosis . ( littaru g . p . & amp ; tiano l ., 2005 ; linnane a . w . et al ., 2002 ; mizuno m . et al ., 1997 ; niklowitz p . et al ., 2002 ). the levels thereof in the human body decrease with advancing age , perhaps because of a decrease in its synthesis or because of the increase in lipid peroxidation which occurs with age . cynarin is an active ingredient contained in the cauline leaves of the common artichoke ( cynaria scolymus ). it chemically derives from the condensation of two units of caffeic acid with a molecule of quinic acid ( 1 , 4 - dicaffeilquinic acid ). in clinical studies , artichoke extracts have shown to improve choleresis and the symptoms of patients suffering from dyspepsia and functional disorders of the liver . cynarin has shown to be effective as a hypolipidemic remedy in a number of clinical studies . cynarin seems to stimulate bile secretion by liver cells and increase the excretion of cholesterol and solid matter in bile . caffeic acid derivatives in general show antioxidant and hepatoprotective effects . cynarin is also hypocholesterolemic , by virtue of its inhibition of cholesterol biosynthesis and inhibition of ldl cholesterol oxidation . moreover , it decreases the betalalpha quotient of lipoprotein and has diuretic effects . the substances specified above , which have a specific hypocholesterolemic action , can also have various types of vitamins or minerals added to them . a food composition containing a combination of the above - mentioned substances , blended in the right doses , has numerous nutritional advantages : it favours the stabilization of cholesterol on correct levels ; it exerts an antioxidant action ; it has an organic anti - oxidative stress action ; it acts to protect the liver ; it protects arteries and the cardio - circulatory system in general ; and it reduces risk of stress - induced avitaminosis . all of the above - described substances are of vegetable origin and are advantageously obtained from extracts of specific plant species which contain one or more of the aforesaid substances . sugar cane ( saccharum officinarum l .) is a tropical plant , native to indo - malaysian regions and belonging to the family poaceae . policosanols , in particular octacosanol , are extracted from sugar cane wax , a byproduct of sugar production . fermented red rice , as already mentioned , is obtained through the fermentation of common cooking rice ( oryza sativa ) by a particular yeast , lo called monascus purpureus or red yeast . during its fermenting activity , this yeast in fact becomes enriched with a group of substances , called monacolins , to which a marked hypocholesterolemic activity has been scientifically attributed . it has been demonstrated that fermented red rice is more effective in terms of hypocholesterolemic action than equivalent doses of lovastatin , evidence that its properties reflect a combination of actions that are not ascribable to monacolin k alone . for this reason as well , in addition to its well - documented hypolipidemic activity , fermented red rice seems to reduce cardiovascular risks thanks to anti - atherosclerotic actions of another type ( anti - inflammatory and vasodilating effects and reduction of lipoprotein a levels ). moreover , fermentation controlled in a laboratory can slightly vary the composition of the monascus purpureus and enable the selection of strains rich in monacolin k or in other substances endowed with particular pharmacological actions . unlike in the case of synthetic statins ( considered veritable drugs ), the use of fermented red rice extracts is also allowed in the production of food supplements , provided that it remains within certain limits established by the ministry . soybean , soy or soya ( glycine max l .) is a herbaceous plant of the family of leguminosae , native to east asia . the part used is the seeds , which contain a high amount of protein , polyunsaturated lipids and glucosides , including isoflavones and saponins . soybean is a leguminous plant like beans , chick peas or lentils , and like all legumes it is rich in b group vitamins , coenzyme q10 , iron and potassium . however , soybeans are more digestible and richer in protein and lipids ( monounsaturated and polyunsaturated and phospholipids like lecithin ) than other legumes . soy protein has a quite good amino acid profile with a biological value of less than 75 , and a protein efficiency ratio of 2 . 1 . medicago sativa l ., known as alfalfa ( from the arabic al - fal - fa , “ father of all foods ”), is a herbaceous plant belonging to the family fabaceae ( or lo leguminosae ). it contains 8 digestive enzymes , phytoestrogens , 40 different bioflavonoids ( with antioxidant , anti - inflammatory and blood vessel reinforcing action ), flavones , glucosides , alkaloids ( a support to antibiotic activity and anti - inflammatories , they favour protein formation ), amino acids , vitamin a , vitamin b9 , vitamin c , vitamin d , vitamin e , vitamin k , minerals , trace elements and high quantities of chlorophyll . caigua is a plant native to peru and belongs to the family cucurbitaceae . its scientific name is cyclanthera pedata , but it is found under many other names : achocha , achokcha , caihua , caygua , cayua , caigua , korila etc . its genus comprises around forty indexed species . the fruit contains peptin , galacturonic acid , dihydroxytryptamine , resins , minerals such as phosphorous , vitamin c , lipoprotein ( sitosterol - 3 - beta - d - glucoside ) and spheroidal components with a hypoglycaemic and anti - ldl cholesterol action . the artichoke ( cynara cardunculus l . ssp . scolymus l .) is a plant of the family asteraceae , cultivated in italy and in other countries for use as a food and , secondarily , for medicinal use . the main component of artichokes , after water , is carbohydrates , among which a distinction is made between inulin and fibres . the main minerals are sodium , potassium , phosphorous and calcium . among vitamins , there is a prevailing presence of b1 and b3 and small amounts of vitamin c . what is more important in explaining the pharmacological activity of artichoke extracts is the presence of a complex of characteristic secondary metabolites : caffeic acid derivatives , including chlorogenic acid , neochlorogenic acid , cryptochlorogenic acid and cynarin ; flavonoids , in particular rutin ; and sesquiterpene lactones : among others , cynaropicrin , dihydrocynaropicrin , grossheimin and cynaratriol . with a functional flour - based food composition containing substances extracted from the above - described plant species , pasta or baked products such as bread , toasting bread , biscuits , crackers , breadsticks , focaccia and lo still others , are advantageously prepared according to the method of the present invention , capable of stabilizing cholesterol and triglyceride levels . according to the present invention , the above - described objects are achieved thanks to the solution specifically referred to in the claims that follow . in relation to the invention , the claims form an integral part of the technical teaching provided . the invention is illustrated in detail below through examples of nonrestrictive embodiments . saccharum oflicinarum 34 mg ( dry extract titrated to 60 % octacosanol ). red rice fermented by monascus purpureus 750 mg ( dry extract titrated to 1 . 5 % plant statins ). glycine max 500 mg ( dry seed extract titrated to 10 % isoflavones ) medicago sativa 50 mg ( alfalfa dry extract ) cyclanthera pedata 58 mg ( dry fruit extract titrated to 35 % sterolic fraction ) cynaria scolymus 1 g ( dry leaf extract ). wheat flour 100 g where all of the above components are in dry powder form . a method for preparing a baked food product envisages the preparation of a dough which comprises : saccharum oficinarum 2 . 3 g ( dry extract titrated to 60 % octacosanol ). red rice fermented by monascus purpureus 10 g ( dry extract titrated to 0 . 4 % monacolin ) glycine max 35 g ( dry seed extract titrated to 10 % isoflavones ) medicago sativa 3 . 5 g ( alfalfa juice concentrate ). cyclanthera pedata 4 g ( dry fruit extract titrated to 35 % sterolic fraction ) cynaria scolymus 50 g ( dry leaf extract ) water 250 ml saccharomyces cerevisiae ( baker &# 39 ; s yeast ) 7 g sodium chloride 5 g powder bread improver 3 . 75 g ( containing wheat flour , malt flour , emulsifier e472e , enzymes , treatment agent e300 ) wheat flour 750 g where the ingredients are added in a kneader which mixes the dough for about 30 minutes . the kneading step is followed by a first rising step , after which the dough is formed into loaves . then follows an additional rising step , after which there is a baking step carried out by placing the loaves in an oven maintained at a temperature of between 180 ″ and 200 ° c . for a period of time ranging between 30 and 45 minutes . during the kneading step , the wheat proteins gliadin and glutenin are joined together to form gluten . as the kneader works , the gluten is disposed in such a way as to form an ordered meshwork interwoven with starch granules . cross - linked meshes are thus formed and the air bubbles subsequently containing carbon dioxide produced by fermentation remain trapped in them . the elastic mesh structure does not allow gas to escape and brings about an increase in the dough volume ( rising ). the kneader operates in such a way as to alternate periods of kneading with periods of rest in order to enable hydration and gluten formation . the kneading step can also take place in a different way than as described above . for example , an alternative kneading method envisages a first step of kneading about 114 to 113 of the total wheat flour with all the baker &# 39 ; s yeast and part of the water necessary ; after the first kneading , there is a first rising step , after which one adds the rest of the flour , water , salt and other ingredients of the food composition of the invention and proceeds to a second kneading step . this method favours development of the leavening , which acts more rapidly , and makes it possible to use a smaller amount of leavening . another variant embodiment of the method for producing the baked products of the invention envisages the use of natural leaven , also called lo sourdough or starter , in the place of baker &# 39 ; s yeast . this is a flour and water dough acidified by a complex of yeast and lactic bacteria which are capable of starting fermentation . unlike baker &# 39 ; s yeast , natural leaven comprises , among the leavening agents , different species of heterofermenting and homofermenting lactic bacteria of the genus lactobacillus . the fermentation of lactic bacteria produces organic acids and also enables greater rising of the product and better digestibility and preservability . some information and definitions regarding the plant species used in the above - described examples of compositions are provided below . functional foods ( bread and other baked products ) were formulated in which phytocomplexes according to the present invention were combined in different fractions . the same were tested on guinea pigs in order to verify whether and to what extent the added phytocomplexes are capable of lo exerting a favourable action in controlling the lipidemic values of the guinea pigs . the results demonstrate that , when they are added to a food like bread , these phytocomplexes are capable of exerting a significant synergistic action in the functional action of the bread itself . the new functional food contains numerous substances capable of exerting an antioxidant action . in particular , it contains : polyphenols , isoflavones , and the oryza sativa ferment ( which synergistically inhibit hepatic cholesterol synthesis ). moreover , the new functional food also contains medicago sativa , which is able to contribute vitamin b9 or folic acid . folic acid is essential for the synthesis of several amino acids , the synthesis of purine and pyrimidine , and the reproduction and growth of cells , in particular the erythrocytes in blood . in this regard , the experimental data showed that medicago sativa extract , included in the formulation of the new functional food ( bread ), performs an action that is synergistic with that of monacolin k ( the vegetable statin formed by monascus purpureus added to the formulation of the functional bread ), as it intervenes in decreasing homocysteine , which is increased as a result of the inhibition of the enzyme hmg - coa reductase , induced , in turn , by the action of the monacolin k that forms during the fermentation of oryza sativa . homocysteine is an amino acid that has drawn great interest from the lo medical scientific community , since an excess thereof can be very dangerous for the body &# 39 ; s health . in fact , hyperhomocysteinemia is considered a major independent risk factor which predisposes to cardiovascular pathologies ( atherosclerosis , myocardial infarction ), cerebrovascular pathologies ( stroke ) and peripheral vascular pathologies ( arterial and venous thrombosis ). it is estimated that individuals affected by hyperhomocysteinemia have double the probability of experiencing vascular incidents compared to those who have values within the range of normality . moreover , the experimental data obtained showed that , by virtue of its octacosanol content , the medicago sativa extract present in the formulation is capable of performing an action of significantly reducing ldl cholesterol , by an extent ranging from 19 to 29 %. the greatest action , in the range of 24 - 29 %, is obtained if it is added to the bread formulation in a form integrated with red rice — monascus purpureus . the bread formulation as described above , in a form supplemented with glycine max extracts , is capable of performing an action of controlling triglycerides and significantly increasing hdl cholesterol ; choline exerts an action synergistically with monascus purpureus and octacosanol , intervening as a coenzyme of metabolism , like b group vitamins , and participating together with inositol in lecithin formation . like choline , inositol , precisely , is one of the constituents of lecithin , participating in its production in the body . inositol takes part in fat metabolism , with a synergistic action associated with monascus purpureus , thus contributing to reducing cholesterol in the blood . furthermore , when integrated with choline , it prevents fats from hardening in the arteries . octacosanol acts directly on the enzyme hmg - coa reductase , reducing the quantity thereof and thus bringing about a lower production of cholesterol , which is thus inhibited by the enzyme itself ; cynara scolymus , when integrated into the bread formulation , is capable of exerting an action on choleretic activity , synergistically with trifolium repens and medicago sativa , by virtue of the organic acids present in it and cynaropricrin , which perform a hypolipidemic action , with an increase in cell - surface receptors for ldl and apolipoproteins a1 and a2 synthesized by the liver , and also of bringing about an increase in hdl . therefore , the functional bread to which vegetable extracts have been added according to the present invention is capable of exerting a functional , salutary action in prevention and treatment of cholesterol and triglyceride levels exceeding the reference values , in addition to an action of protecting the arteries and the cardio - circulatory system and a hepatoprotective and antioxidant action , thanks to the synergistic biochemical actions of the phytocomplexes included in the bread , which enable an enhanced bioactivity thereof to be obtained in the resulting product . canetti m et al . “ a two - year study on the efficacy and tolerability of policosanol in patients with type i1 hyperlipoproteinaemia .” int j clin pharmacol res 15 ( 4 ): 159 - 65 , 1995 . mas r et al . “ effects of policosanol in patients with type ii hypercholesterolemia and additional coronary risk factors .” clin pharmacol ther 65 ( 4 ): 439 - 47 , 1999 . castano g et al . “ effects of policosanol in older patients with type i ! hypercholesterolemia and high coronary risk .” j gerontol a biol sci med sci 56 ( 3 ): m186 - 92 , 2001 . castano g et al . “ effects of policosanol on older patients with hypertension and type i1 hypercholesterolaemia .” drugs r d 3 ( 3 ): 159 - 72 , 2002 . gouni - berthold the et al . “ policosanol : clinical pharmacology and lo therapeutic significance of a new lipid - lowering agent .” am heart j 143 ( 2 ): 356 - 65 , 2002 . castano g et al . “ effects of policosanol and pravastatin on lipid profile , platelet aggregation and endothelemia in older hypercholesterolemic patients .” int j clin pharmacol res 19 ( 4 ): 105 - 16 , 1999 . castano g et al . “ comparison of the efficacy and tolerability of policosanol with atorvastatin in elderly patients with type i1 hypercholesterolaemia .” drugs aging 20 ( 2 ): 15 3 - 63 , 2003 . arruzazabala , m . l . ; 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