Patent Abstract:
The invention relates to a particle injector for introducing particles into a carrier flow of a microfluidic system, especially for injecting biological cells into the carrier flow of a cell sorter. The particle injector includes an inlet for receiving the carrier flow, an outlet for discharging the carrier flow including the introduced particles, a carrier flow channel which connects the inlet to the outlet, and an injection channel flowing into the carrier flow channel for introducing the particles into the carrier flow. The inventive particle injector is characterized in that the carrier flow channel has substantially no dead volume.

Full Description:
BACKGROUND OF THE INVENTION 
     The invention relates to a particle injector for introducing particles into a carrier flow of a microfluidic system, in particular for injecting biological cells into the carrier flow of a cell sorter, according to the preamble of claim  1 . 
     U.S. Pat. No. 5,489,506 discloses a cell sorter which enables biological cells to be separated dielectrophoretically in a carrier flow, whereby the dielectrophoretic effects used for separating are described for example in MÜLLER, T. et al. : “A 3-D microelectrode system for handling and caging single cells and particles”, Biosensors &amp; Bioelectronics 14 (1999) 247-256. The biological cells to be sorted are hereby injected by a particle injector into the carrier flow, whereby the carrier flow enters the particle injector via an inlet and later leaves it along with the injected biological cells via an outlet. The actual injecting of the biological cells to be sorted takes place through an injection needle, which is stuck through a septum in the particle injector and is guided coaxially into the carrier flow between the inlet and the outlet of the particle injector, so that the cells introduced via the injection needle are carried along by the carrier flow. 
     The disadvantage to this known particle injector is the loss of cells, arising from cell depositing in the particle injector. In the extreme case these cell deposits can result in clogging of the particle injector, impairing the feed of the carrier flow or to total obstruction. This has a particularly strong effect in fluidic systems with minimal feed rates of e.g. less than 200 μl/h. 
     The object of the invention therefore is to minimize the loss of cells through particle depositing in the above described known particle injector to prevent obstruction of the particle injector. 
     SUMMARY OF THE INVENTION 
     In particular a particle injector is to be provided, which selectively enables continuous or discontinuous injection of particles in a fluidic microchip (“Lab-on-Chip”), whereby the most uniform possible incessant (e.g. in the range of hours), loading of the system with particles is achieved. In addition, scattering of the particles is also ensured, thus counteracting interfering aggregate formation. 
     So as to prevent obstruction of the particle injector the carrier flow channel between the inlet of the particle injector and the outlet of the particle injector preferably has no dead volume, to avoid particles being stopped in the flow channel. 
     The carrier flow channel of the particle injector therefore preferably has a smooth inner contour without projections or depressions, which could hinder a laminar flow course. When considered as mathematically idealized the inner contour of the carrier flow channel therefore preferably has a constantly differentiable top surface. 
     The carrier flow channel in the particle injector between the inlet and the outlet preferably even has a constant cross-section of flow, since each change in cross-section in the carrier flow channel facilitates particles being stopped. 
     The cross-section of the carrier flow channel is preferably circular, however with the inventive particle injector the carrier flow channel can also be formed elliptical or angular. 
     In the preferred embodiment of the invention the injection channel for injecting the particles terminates obtusely and preferably right-angled in the carrier flow channel, so that the particle injector can also be described as a T injector. The advantage of such a geometric arrangement of the injection channel is that the carrier flow flowing in the carrier flow channel carries along the particles to be injected. The invention is however not limited with respect to the geometric arrangement of the injection channel to obtuse confluence of the injection channel in the carrier flow channel. It is also possible for example that the injection channel, as explained for the abovementioned U.S. Pat. No. 5,489,506, runs coaxially to the carrier flow channel so as to inject the particles coaxially into the carrier flow. 
     With the inventive particle injector the injection channel preferably serves not only for injecting the particles, but also for mechanical guiding of an injection needle, which can be stuck for example in through a septum and guided into the injection channel. The injection channel therefore preferably has an inner diameter, which is slightly greater than the outer diameter of the injection needle. With the injection channel of the particle injector the injection needle preferably forms a loose fit or transition fit to achieve good mechanical guiding of the injection needle. 
     Inserting the injection needle into the injection channel can be made easier in the inventive particle injector by a feeding-in aid, preferably comprising funnel-shaped cross-sectional widening of the injection channel. The feeding-in aid for the injection needle is preferably arranged in a separate component, attached detachably to the particle injector. By way of example this component serving as feeding-in aid can be screwed separately onto the particle injector or connected in some other way to the particle injector. By way of alternative however it is also possible that the feeding-in aid is arranged monobloc on the particle injector, so that a separate component as feeding-in aid can be dispensed with. 
     The abovementioned septum for sealing off the injection channel is preferably exchangeable and constructed multilayer. By way of example the septum can have a silicon core, coated on both sides with Teflon. 
     The fluidic contacting of the inventive particle injector occurs preferably by way of hoses, which are fastened on the inlet or respectively the outlet of the particle injector. With this fluidic contacting it is desirable that at the transition point between the hoses and the carrier flow channel as far as possible no cross-sectional leaks occur, so as to prevent depositing of particles there. To facilitate correct mounting of the hoses the inventive particle injector therefore preferably has at the inlet and/or the outlet a centering aid so that the hose is mounted as coaxially as possible to the carrier flow channel. 
     Such a centering aid can for example comprise a substantially hollow-cylindrical pick-up, which borders the carrier flow channel and is arranged coaxially to the carrier flow channel, whereby the inner diameter of the pick-up is greater by the wall thickness of the line to be connected than the inner diameter of the carrier flow channel. The line is therefore inserted into the hollow-cylindrical pick-up, which runs coaxially to the carrier flow channel and thereby ensures corresponding coaxial alignment to the line. 
     In a variant of the invention injecting the particles into the carrier flow channel takes place with respect to the gravity acting on the particle injector from top to bottom preferably vertically, whereby the injection channel is arranged on the top side of the particle injector. With such an arrangement of the injection channel above the carrier flow channel the effect of gravity favors introducing the particles into the carrier flow channel. 
     Here it is possible that the cross-section of the injection channel tapers conically down to the carrier flow channel, which also supports introducing an injection needle into the injection channel. In addition to this, the conical tapering of the injection channel also has a funneling function, as the particles converge in the lower region of the injection channel, so that no or only some particles remain caught in the injection channel, guaranteeing continuous particle feeding. 
     By way of example, the injection channel can taper to the carrier flow channel with a conic angle between 5° and 45°, whereby any intermediate values are possible. 
     In another variant of the invention the inlet of the carrier flow channel on the other hand is arranged on the underside of the particle injector, while the outlet of the carrier flow channel is located on the top side of the particle injector, so that the carrier flow is directed from the bottom to the top. The injection channel can hereby terminate to the side in the carrier flow channel, whereby the carrier flow channel preferably has a cross-section, which widens out from the inlet to the outlet. By way of example, the carrier flow channel can narrow conically to the inlet with a conic angle of between 5° and 45°, whereby any intermediate values are possible. Such narrowing of the cross-section of the carrier flow channel to the subjacent inlet is advantageous, since this counteracts any occluding of the carrier flow channel. In this way sedimentation effects in the carrier flow channel could lead to particle deposits in the lower region of the carrier flow channel. The narrowing of the cross-section in the lower region of the carrier flow channel however leads to a corresponding increase in the flow rate, thus extensively avoiding sedimentation deposits with the danger of occlusion. 
     The carrier flow channel between the inlet and the outlet preferably has a volume of between 0.02 μl and 5 μl, where any intermediate values are possible. Though there is also the possibility that the volume of the carrier flow channel between the inlet and the outlet is between 20 μl and 50 μl, whereby likewise any intermediate values are possible. Furthermore, this volume can even be up to 1 ml or more, with volumes of between 0.02 μl and more than 1 ml possible. 
     There is also the possibility that the injection channel terminates obliquely upwards in the carrier flow channel, whereby the carrier flow channel preferably runs vertically. With the carrier flow channel flowing through from bottom to top the suspended particles are then carried along upwards and are flushed out of the particle injector. The angle between the injection channel and the carrier flow channel can hereby for example be between 10° and 80°, whereby any intermediate values are possible. 
     In addition to this, an agitation chamber, in which a magnetic stirring rod is located, can be arranged in the particle injector. This advantageously enables the carrier flow with the particles suspended therein in the agitation chamber to be intermixed with a conventional magnetic stirrer. 
     Several inlets and/or several outlets for the carrier flow can be arranged parallel to one another. In addition to this, there is also the possibility for several particle inlets to be provided. 
     The inventive particle injector can also have two carrier flow inlets, via which the two carrier flows are fed, whereby both carrier flow inlets preferably terminate in a single carrier flow outlet. Both the carrier flow inlets can hereby be arranged laterally and opposite one another. 
     It is a further advantage if the carrier flow channel in the particle injector is guided meandering between the inlet and the outlet. Due to the narrowing and widening in the carrier flow channel the sedimentizing of the particles in the carrier flow channel is countered, so that the suspended particles move uniformly and continuously. 
     It should also be mentioned that the inventive particle injector can preferably be autoclaved so as to enable sterilization of the particle injector. A suitable material for the particle injector therefore is preferably PEEK, however the inventive particle injector can also comprise other materials. 
     It is also advantageous if the particle injector comprises a heat-conductive material, so as to measure or influence the temperature of the particle injector. The particle injector is preferably therefore connected to a temperature sensor and/or a tempering element, whereby the tempering element preferably enables both heating and also cooling of the particle injector and for example may comprise a Peltier element. 
     The inventive particle injector can be made for example by machining methods or an injection molding process, however the invention is not limited to these particular manufacturing methods. 
     In addition to this, the invention also comprises a microfluidic system with the inventive particle injector, whereby the particle injector is preferably arranged in a carrier flow line, terminating in a cell sorter. 
     In an embodiment of such a microfluidic system several inventive particle injectors can be arranged in the carrier flow line behind one another, so that different particles can be injected successively. Instead of particles specific reagents or reaction solutions can also be added via the individual particle injectors in each case. 
     It should also be mentioned that the term particle used within the scope of the invention is to be understood generally, and is not limited to individual biological cells. Rather, the inventive particle injector can operate with various types of particles, in particular synthetic or biological particles. Specific advantages will emerge if the particles include biological materials, therefore for example biological cells, cell groups, cell constituents or biologically relevant macromolecules, in each case if required in combination with other biological particles or synthetic carrier particles. 
     Synthetic particles can include solid particles, liquid particles separated out from the suspension medium, or multi-phase particles, which form a separate phase relative to the suspension medium in the carrier flow channel. 
    
    
     
       BRIEF DESCRIPTION OF SEVERAL VIEWS OF THE DRAWINGS 
       Other advantageous further developments of the invention are characterized in the independent claims or are explained in greater detail hereinbelow along with the description of the preferred embodiments of the invention by way of the figures, in which: 
         FIG. 1  illustrates a cell sorter with an inventive particle injector, 
         FIGS. 2 to 4  illustrate cross-sectional views of various alternative embodiments of the particle injector, 
         FIG. 5  illustrates a side elevation of a feeding-in aid for easing insertion of an injection needle into the inventive particle injectors, 
         FIG. 6  illustrates a variant of a microfluidic system with an inventive particle injector, 
         FIG. 7  illustrates a further embodiment of an inventive particle injector with integrated magnetic stirring rods, 
         FIG. 8  illustrates a further embodiment of an inventive particle injector with angled guiding of the carrier flow, 
         FIG. 9  illustrates an embodiment of an inventive particle injector, in which the particles are injected obliquely into the carrier flow, 
         FIG. 10  illustrates another embodiment of an inventive particle injector with two opposing carrier flow feeds, 
         FIG. 11  illustrates a perspective illustration of an inventive particle injector, and 
         FIG. 12  illustrates a further embodiment of an inventive particle injector with meandering guiding of the carrier flow channel. 
     
    
    
     DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS 
     The schematic illustration in  FIG. 1  shows an inventive cell sorter, which sorts biological cells dielectrophoretically by means of a microfluidic sorter chip  1 . 
     The techniques of the dielectrophoretic influence of biological cells are described for example in MÜLLER T. et al. : “A 3-D microelectrode system for handling and caging single cells and particles”, Biosensors &amp; Bioelectronics 14 (1999) 247-256, so that a detailed description of the dielectrophoretic processes in the sorter chip  1  are dispensed with hereinbelow, and this is pointed out with respect to the above publication. 
     The sorter chip  1  has several terminals  2 - 6  for fluidic contacting whereby fluidic contacting of the terminals  2 - 6  is described in DE 102 13 272, the content of which is incorporated herein by reference. 
     The terminal  2  of the sorter chip  1  serves to receive a carrier flow with the biological cells to be sorted, while the terminal  3  of the sorter chip  1  serves to discard the selected biological cells, which are no longer being inspected on the sorter chip  1 . The selected biological cells can be intercepted by an injection  7 , which can be connected to the terminal  3  of the sorter chip  1 . The output  5  of the sorter chip  1  on the other hand serves to reject the interesting biological cells, which are then further processed or inspected. 
     The purpose of the terminals  4  and  6  of the sorter chip  1  is to feed a so-called shell flow, whereof the task is to guide the selected biological cells to the terminal  5  of the sorter chip  1 . With respect to the functioning of the shell flow reference is made to the German patent application DE 100 05 735, so that a detailed description of the functioning of the shell flow can be omitted. 
     The terminals  4  and  6  of the sorter chip are connected via two shell flow lines  8 ,  9 , a Y piece  10  and a four-way valve  11  with a pressurized container  12 , in which there is a cultivation medium for the shell flow. Instead of the cultivation medium, however, in the pressurized container  12  there can also be a so-called manipulation buffer. 
     The pressurized container  12  is set on a compressed air line  13  at superpressure, so that with corresponding adjustment of the four-way valve  11  the cultivation medium in the pressurized container  12  flows via the Y piece  10  and the shell flow lines  8 ,  9  to the terminals  4 ,  6  of the sorter chip  1 . 
     The terminal  2  of the sorter chip  1  by way of comparison is connected via a carrier flow line  14  to a particle injector  15 , whereof various alternative embodiments are illustrated in  FIGS. 2 to 4  and are described hereinbelow in greater detail. 
     Upstream the particle injector  15  is connected via a T piece  16  to a carrier flow injection  17 , driven by machine and injecting a preset liquid flow of a carrier flow. 
     In addition to this, the T piece  16  upstream is connected via a further four-way valve  18  and a shell flow line  19  to a three-way valve  20 . The three-way valve  20  enables flushing of the shell flow lines  8 ,  9  as well as the carrier flow line  14  prior to actual running. 
     For this purpose the three-way valve  20  upstream is connected via a peristaltic pump  21  to three three-way valves  22 . 1 - 22 . 3 , to which in each case an injection reservoir  23 . 1 - 23 . 3  is attached. The injection reservoirs  23 . 1 - 23 . 3  hereby serve to feed a filling flow for flushing the entire fluidics system prior to actual operation, whereby the injection reservoir  23 . 1  contains 70% ethanol, whereas the injection reservoir  23 . 2  contains Aqua destillata as filling flow substance. The injection reservoir  23 . 3  finally contains a buffer solution as filling flow substance, whereby alternatively another manipulation solution can also be used as filling flow substance, such as for example a physiological saline solution. 
     Also, the cell sorter has a collection container  27  for excess shell flow as well as a collection container  28  for excess filling flow. 
     Hereinafter the flushing procedure is first described, which is carried out prior to actual operation of the cell sorter so as to free the shell flow line  8 ,  9 , the carrier flow line  14  and the remaining fluidics system of the cell sorter of air bubbles and contaminants. 
     For this purpose first the three-way valve  22 . 1  is opened and ethanol is injected from the injection reservoir  23 . 1  as a filling flow, whereby the ethanol is conveyed by the peristaltic pump  21  first to the three-way valve  20 . During the flushing procedure the three-way valve  20  is adjusted such that part of the filling flow forwarded by the peristaltic pump  21  is conveyed via the filling flow line  19 , while the remaining portion of the filling flow conveyed by the peristaltic pump  21  reaches the four-way valve  11 . Both four-way valves  11 ,  18  are again adjusted such that the filling flow is lead through the shell flow lines  8 ,  9  and the carrier flow line  14 . Cultivation medium flows from the pressurized container  12  into the collection container  27  to briefly inundate the lines. 
     After the above described flushing of the cell sorter with ethanol flushing with Aqua destillata or respectively buffer solution takes place in the same way, whereby in each case the three-way valves or respectively  22 . 2  or respectively  22 . 3  are opened. 
     With the above described flushing procedure excess filling flow can be diverted by the four-way valve  18  to the collection container  28 . 
     Following the flushing procedure the three-way valves  22 . 1 - 22 . 3  are closed and the peristaltic pump  21  is switched off. 
     To introduce the sorting operation the four-way valve  11  is adjusted such that the pressurized container  12  is connected to the Y piece  10 , such that the cultivation medium in the pressurized container  12  is pressed into the shell flow lines  8 ,  9  on account of the excess pressure prevailing in the pressurized container  12 . 
     Further to this, during the sorting operation the four-way valve  18  is adjusted such that there is no flow connection between the T piece  16  and the four-way valve  18 . 
     The carrier flow injected by the carrier flow injection  17  then flows via the T piece  16  into the particle injector  15 , whereby biological cells are injected into the carrier flow by a further injection  29 . Next the carrier flow flows with the injected biological cells from the particle injector  15  via the carrier flow line  14  to the terminal  2  of the sorter chip. 
     It should also be mentioned that attached to the particle injector  15  is a temperature sensor  30  for measuring the temperature T of the particle injector  15 . 
     In addition to this, a tempering element  31  in the form of a Peltier element, for heating or cooling the particle injector  15 , is located on the particle injector  15 . 
     The heating or respectively cooling energy Q is hereby preset by a temperature controller  32 , which is connected at the inlet side to the temperature sensor  30  and resets the temperature T of the particle injector  15  to a preset nominal value. 
     The embodiment of the particle injector  15  illustrated in  FIG. 2  will now be described hereinbelow. 
     The particle injector  15  has a basic body  33  made of PEEK, which can be autoclaved and thus enables easy and/or multiple sterilization. 
     For taking up the carrier flow the particle injector  15  has an inlet  34  with an inner thread  35 , into which a screw flange of a terminal hose  36  can be screwed, with the screw flange not being illustrated here for the sake of clarity. 
     For discharging the carrier flow with the injected biological cells the particle injector  15  has an outlet  37  with an inner thread  38 , in which likewise a screw flange of a terminal hose  39  can be screwed, with the screw flange of the terminal hose  39  likewise not being illustrated here for the sake of clarity. 
     To make mounting of both hoses  36 ,  39  easier the particle injector  15  in each case has a centering aid  40 ,  41 , comprising a cylindrical pick-up and bordering the inlet  34  or respectively  37 . Running between both centering aids  40 ,  41  is a carrier flow channel  42  coaxially to both centering aids  40 ,  41 , whereby the inner diameter of both centering aids  40 ,  41  is larger by the wall thickness of both connecting hoses  36 ,  39  than the inner diameter of the carrier flow channel  42 . With mounting the connecting hoses  36 ,  39  the former are therefore placed in the centering aids  40 ,  41  such that at the point of impact between the hoses  36 ,  39  and the carrier flow channel  42  no leaks occur, which extensively prevents occlusion of the carrier flow channel  42 . 
     In the carrier flow channel  42  an injection channel  43 , into which an injection needle of the injection  29  can be introduced for injecting biological cells, terminates at a right angle to the carrier flow channel  42 , whereby the injection needle of the injection  29  punctures a septum  44 . 
       FIG. 3  shows an alternative embodiment of an injector  15 ′, which substantially matches with the above described embodiment illustrated in  FIG. 2 . In the interests of avoiding repetition reference is therefore made hereinbelow to the above described description to  FIG. 2 , whereby the same reference numerals are used as in  FIG. 2  for corresponding parts, which are distinguished for differentiating only by an apostrophe. 
     A particularity of the particle injector  15 ′ comprises the inlet  34 ′ for the carrier flow being arranged on the underside of the particle injector  15 ′, while the outlet  37 ′ for the carrier flow with the injected biological cells being located on the top side of the particle injector  15 ′. The carrier flow therefore runs in the particle injector  15 ′ vertically from bottom to top, whereby the injection channel  43 ′ terminates to the side in the carrier flow channel  42 ′. 
     A further particularity of the particle injector  15 ′ is that the cross-section of the carrier flow channel  42 ′ tapers from top to bottom, so that the flow rate of the carrier flow in the carrier flow channel  42 ′ accordingly increases from top to bottom. Sedimentation deposits on the underside of the carrier flow channel  42 ′ are counteracted by this increase in the flow rate in the carrier flow channel  42 ′. 
     There is also the possibility that at the lower end of the funnel-shaped narrowing of the injection channel  43 ′ just above the carrier flow channel  42 ′ there is a valve arranged, enabling discontinuous particle feeding. 
       FIG. 4  shows another alternative embodiment of a particle injector  15 ″, which likewise substantially matches the above described particle injector  15  shown in  FIG. 2 . To avoid repetition therefore hereinbelow reference is also made to the above description to  FIG. 2 , whereby the same reference numerals are used for corresponding parts, which are distinguished for differentiating only by two apostrophes. 
     A particularity of the particle injector  15 ″ comprises the cross-section of the injection channel  43 ″ widening upwards to its terminal opening, so that the injection needle of the injection  29  can be introduced more easily. 
     In addition to this, the conical narrowing of the injection channel  43 ″ also has a funnel function, since the particles converge in the lower region of the injection channel  43 ″, so that no or only some particles remain in the injection channel  43 ″, ensuring continuous particle feeding. 
     The cross-sectional widening of the injection channel  43 ″ further offers the advantage that the injection channel  43 ″ has an additional injection volume in the range of 5-100 μl. 
     Finally,  FIG. 5  shows an exemplary feeding-in aid  45  for the injection needle of the injection  29 , whereby the feeding-in aid  45  is designed as a separate component. The feeding-in aid  45  has on its underside a cylindrical section  46  with an external thread  47 , which can be screwed into a corresponding inner thread of the particle injector  15 ′ or respectively  15 ″, in order to attach the feeding-in aid  45  on the particle injector  15 ′ or respectively  15 ″. 
     The feeding-in aid  45  is screwed in manually via knurling  48 , arranged on an upper section of the feeding-in aid  45 . 
     In the feeding-in aid is a projection  49  of the injection channel  43  or respectively  43 ′, which transitions at its top side into a funnel-shaped widening  50 , to facilitate introducing the injection needle of the injection  29 . 
       FIG. 6  finally shows a modification of the region outlined in dashed lines in  FIG. 1 , so that hereinbelow reference is made to the description to  FIG. 1  to avoid repetition. In addition to this, the same reference numerals, which are distinguished to avoid repetition only by additional indices, are used for corresponding components. 
     A particularity of this modification comprises three particle injectors  15 . 1 - 15 . 3  being arranged successively in the carrier flow line  14 ′, so that three different particles can be injected into the carrier flow. 
       FIG. 7  shows a further embodiment of an inventive particle injector  51  with an inlet  52  for receiving a carrier flow and an outlet  53  for discharging the carrier flow with particles suspended therein. 
     The inlet  52  terminates in the particle injector  51  in an agitation chamber  54 , in which a magnetic stirring rod is located, not illustrated here for the sake of clarity. The carrier fluid in the agitation chamber  54  can therefore be agitated by a conventional magnetic stirrer, resulting in thorough intermingling of the carrier fluid with the particles suspended therein. The agitation rate is hereby selected such that the particles suspended in the carrier fluid are not damaged by the stirring procedure. 
     The particle injector  51  comprises a lower part  55  and an upper part  56 , whereby the agitation chamber  54  is arranged in the lower part  55 . In the mounted state the lower part  55  is connected firmly to the upper part  56  and sealed by an O ring located in between. 
     The particles are injected into the carrier flow via an injection channel  57 , which terminates in the agitation chamber  54  to the side near the outlet  53 ′. The injection channel  57  can hereby be closed by a septum, as already described hereinabove. 
     In this embodiment the inlet  52  for the carrier flow is on the underside of the particle injector  51 , whereas the outlet  53  is arranged on the top side, so that the carrier flow flows through the particle injector  51  from bottom to top. 
     Alternatively, however, it is also possible that the inlet  52  is arranged on the top side of the particle injector  51 , while the outlet  53  is located on the underside of the particle injector  51 , such that the carrier flow slows through the particle injector  51  from top to bottom. 
     Hereby, parallelizing is also possible and between the agitation chamber  54  and the outlet  53  a valve can be arranged to enable discontinuous discharge. 
       FIG. 8  shows a further embodiment of an inventive particle injector  58  with an inlet  59  for receiving a carrier flow and an outlet  60  for discharging the carrier flow with particles suspended therein. 
     The inlet  59  is hereby arranged on the left side of the particle injector  58 , while the outlet  60  is located on the underside of the particle injector  58 . The carrier flow is therefore deflected down into the particle injector  58  by 90°. 
     For particle injection the particle injector  58  has an injection terminal  61 , arranged on the top side of the particle injector  58  and closed by a septum  62 . The septum  62  is penetrated by an injection needle for injecting particles into the carrier flow. 
     Located under the septum  62  in the particle injector  58  are a cylindrical sedimentation space  63 , in which the suspended particles illustrated by hatching  64  sedimentize downwards due to gravity, and enter the carrier flow depending on the sedimentation rate. The sedimentation space  63  can however alternatively be designed conically. 
       FIG. 9  shows a further embodiment of an inventive particle injector  65  with an inlet  66  for the carrier flow and an outlet  67  for discharging the carrier flow with the particles suspended therein. 
     The inlet  66  for the carrier flow is located on the underside of the particle injector  65 , while the outlet  67  is arranged on the top side, so that the carrier flow flows through the particle injector  65  from bottom to top. 
     The inlet  66  is connected via a carrier flow channel  68  to the outlet  67 , whereby an injection channel  69 , which goes out from an injection terminal  70 , terminates in the carrier flow channel  68  obliquely from above, whereby the injection terminal  70  is closed by a septum  71  in the above described manner. 
     A particle suspension, which is distributed in the long-stretched-out injection channel  69 , is injected through the injection terminal  70 . Due to gravity the particles begin to sink. A jet, which already receives sunken and other still sinking particles and flows upwards out of the particle injector  65 , is formed by the carrier flow, which enters the particle injector  65  from below and via the narrowing of the carrier flow channel  68 , as shown. In the long-stretched-out carrier flow channel  68  the resulting carrier flow rates and injected volumes can vary, depending on length and diameter. 
       FIG. 10  shows a further embodiment of an inventive particle injector  72  with two laterally arranged, opposing inlets  73 ,  74  for receiving two carrier flows, whereby both inlets  73 ,  74  terminate in the middle of the particle injector  72  into a perpendicular cylindrical injection channel  75 . 
     The injection channel  75  goes from an injection terminal arranged on the top side of the particle injector  72   76  and terminates on the underside of the particle injector  72  in an outlet  77  for discharging the carrier flow with the particles suspended therein. 
       FIG. 11  shows a perspective illustration of a further embodiment of an inventive cuboid particle injector  78  with an inlet  79  for receiving a carrier flow and an outlet  80  for discharging the carrier flow with particles suspended therein, whereby the inlet  79  inside the particle injector  78  is connected to the outlet  80  by a carrier flow channel. 
     The inlet  79  is hereby located on the side of the particle injector  78  in the lower third, whereas the outlet  80  is arranged centrally on the top side of the particle injector  78 . 
     Situated on the front side of the particle injector  78  is an injection terminal  81 , by means of which particles can be injected into the carrier flow. 
       FIG. 12  finally shows an embodiment of an inventive particle injector  82  with a meandering guide for a carrier flow channel  83  between an inlet  84  and an outlet  85 . 
     Terminating in the meandering carrier flow channel  83  is an injection terminal  86 , via which particles can be injected into the carrier flow. Due to the narrowing and widening in the carrier flow channel  83  the sedimentizing of particles in the carrier flow channel  83  is countered, so that the suspended particles move uniformly and continuously. 
     The invention is not limited to the above described preferred embodiments. Rather a plurality of variants and modifications is possible, which can likewise make use of the inventive idea and therefore fall within the range of protection.

Technology Classification (CPC): 6