Abstract:
Nucleotide sequences for two genes, tcdB and tccC2, from the tcd genomic region of  Photorhabdus luminescens  W-14 are useful in heterologous expression of orally active insect toxins.

Description:
RELATED APPLICATIONS  
       [0001]    This application claims priority from U.S. Provisional Patent Application Ser. No. US 60/191806 filed March 24, 2000. 
     
    
     
       BACKGROUND OF THE INVENTION  
         [0002]    As reported in W098/08932, protein toxins from the genus Photorhabdus have been shown to have oral toxicity against insects. The toxin complex produced by  Photorhabdus luminescens  (W-14), for example, has been shown to contain ten to fourteen proteins, and it is known that these are produced by expression of genes from four distinct genomic regions: tca, tcb, tcc, and tcd. W098/08932 discloses nucleotide sequences for many of the native toxin genes.  
           [0003]    Of the separate toxins isolated from  Photorhabdus luminescens  (W-14), those designated Toxin A and Toxin B have been the subject of focused investigation for their activity against target insect species of interest, for example corn rootworm. Toxin A is comprised of two different subunits. The native gene tcda (SEQ ID NO:1) encodes protoxin TcdA (see SEQ ID NO:1). As determined by mass spectrometry, TcdA is processed by one or more proteases to provide Toxin A. More specifically, TcdA is an approximately 282.9 kDA protein (2516 aa) that is processed to provide TcdAii, an approximately 208.2 kDA (1849 aa) protein encoded by nucleotides 265-5811 of SEQ ID NO:1, and TcdAiii, an approximately 63.5 kDA (579 aa) protein encoded by nucleotides 5812-7551 of SEQ ID NO:1.  
           [0004]    WO 01/11029 discloses nucleotide sequences that encode TcdA and TcbA and have base compositions that have been altered from that of the native genes to make them more similar to plant genes. Also disclosed are transgenic plants that express Toxin A and Toxin B.  
           [0005]    We have observed that heterologous expression of Toxin A does not afford the level of oral toxicity to insects that is observed for the native toxin. It would be very valuable if means could be found to enhance the level of toxicity of heterologously expressed Toxin A.  
         SUMMARY OF THE INVENTION  
         [0006]    The present invention provides nucleotide sequences for two genes, tcdB and tccC2, from the tcd genomic region of  Photorhabdus luminescens  W-14. These sequences were not previously known. We have discovered that coexpression of tcdB and tccC2 with tcdA in heterologous hosts results in enhanced levels of oral insect toxicity compared to that obtained when tcdA is expressed alone in such heterologous hosts. Coexpression of tcdB and tccC2 with tcdA or tcbA, or with any other functionally equivalent toxin gene in the same family as tcdA and tcbA, to provide enhanced oral insect activity falls within the scope of the invention.  
           [0007]    Summary of the Sequences  
           [0008]    SEQ ID NO: 1 is the DNA sequence for tcdA from  Photorhabdus luminescens  W-14.  
           [0009]    SEQ ID NO: 2 is the amino acid sequence for TcdA from  Photorhabdus luminescens  W-14.  
           [0010]    SEQ ID NO: 3 is the DNA sequence for tcdB from  Photorhabdus luminescens  W-14.  
           [0011]    SEQ ID NO: 4 is the amino acid sequence for TcdB from  Photorhabdus luminescens  W-14.  
           [0012]    SEQ ID NO: 5 is the DNA sequence for tccC2 from  Photorhabdus luminescens  W-14.  
           [0013]    SEQ ID NO: 6 is the amino acid sequence for TccC2 from from  Photorhabdus luminescens  W-14.  
           [0014]    SEQ ID NO: 7 is the DNA sequence for tcbA from from  Photorhabdus luminescens  W-14.  
           [0015]    SEQ ID NO: 8 is the amino acid sequence for TcbA from  Photorhabdus luminescens  W-14. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION  
       [0016]    It is preferred for the nucleic acids according to the invention to comprise at least one sequence chosen from  
         [0017]    (a) the sequences according to SEQ ID NOS: 3 and 5.  
         [0018]    (b) at least 14 base pairs-long partial sequences of the sequences defined under (a),  
         [0019]    (c) sequences that hybridize with the sequences defined under (a),  
         [0020]    (d) sequences that are at least 70%, preferably 80% and even more preferred, 90% identical to the sequences defined under (a),  
         [0021]    (e) sequences that are at least 70%, preferably 80% and even more preferred, 90% similar to the sequences defined under (a),  
         [0022]    (f) sequences that complement the sequences defined under (a), and  
         [0023]    (g) sequences that due to the degeneracy of the genetic code, code for the same amino acid sequence as(i.e. are “isocoding” with) the sequences defined under (a) through (e).  
         [0024]    The expression “hybridize” as used herein refers to hybridization under the following specified conditions: 5×SSC; blocking reagent (Roche Diagnostics Inc., Mannheim, Germany), 1%; N-lauroyl-sarcosine, 0.1%; SDS (sodium-dodecyl sulfate) 0.02%; hybridization temperature: 60° C.; first wash step: 2×SSC at 60° C.; second wash step: 2×SSC at 60° C.; preferred second wash step: 0.5×SSC at 60° C.; especially preferred second wash step: 0.2×SSC at 60° C.  
         [0025]    “Identity” and “similarity” are scored by the GAP algorithm using the Blosum 62 protein scoring matrix (Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison, Wis.).  
         [0026]    Expression of the Nucleotide Sequences in Heterologous Microbial Hosts  
         [0027]    As biological insect control agents, the insecticidal toxins are produced by expression of the nucleotide sequences in heterologous host cells capable of expressing the nucleotide sequences. In a first embodiment, additional copies of one or more of the nucleotide sequences are added to  Xenorhabdus nematophilus, Xenorhabdus poinarii,  or  Photorhabdus luminescens  cells either by insertion into the chromosome or by introduction of extrachromosomally replicating molecules containing the nucleotide sequences.  
         [0028]    In another embodiment, at least one of the nucleotide sequences of the invention is inserted into an appropriate expression cassette, comprising a promoter and termination signals. Expression of the nucleotide sequence is constitutive, or an inducible promoter responding to various types of stimuli to initiate transcription is used. In a preferred embodiment, the cell in which the toxin is expressed is a microorganism, such as a virus, a bacteria, or a fungus. In a preferred embodiment, a virus, such as a baculovirus, contains a nucleotide sequence of the invention in its genome and expresses large amounts of the corresponding insecticidal toxin after infection of appropriate eukaryotic cells that are suitable for virus replication and expression of the nucleotide sequence. The insecticidal toxin thus produced is used as an insecticidal agent. Alternatively, baculoviruses engineered to include the nucleotide sequence are used to infect insects in-vivo and kill them either by expression of the insecticidal toxin or by a combination of viral infection and expression of the insecticidal toxin.  
         [0029]    Bacterial cells are also hosts for the expression of the nucleotide sequences of the invention. In a preferred embodiment, non-pathogenic symbiotic bacteria, which are able to live and replicate within plant tissues, so-called endophytes, or non-pathogenic symbiotic bacteria, which are capable of colonizing the phyllosphere or the rhizosphere, so-called epiphytes, are used. Such bacteria include bacteria of the genera Agrobacterium, Alcaligenes, Azospiriilum, Azotobacter, Bacillus, Ciavibacter, Enterobacter, Erwinia, Flavobacter, Klebsielia, Pseudomonas, Rhizobium, Serratia, Streptomyces and Xanthomonas. Symbiotic fungi, such as Trichoderma and Gliocladium are also possible hosts for expression of the inventive nucleotide sequences for the same purpose.  
         [0030]    Techniques for these genetic manipulations are specific for the different available hosts and are known in the art. For example, the expression vectors pKK223-3 and pKK223-2 can be used to express heterologous genes in  E. coli,  either in transcriptional or translational fusion, behind the tac or trc promoter. For the expression of operons encoding multiple ORFS, the simplest procedure is to insert the operon into a vector such as pKK2233 in transcriptional fusion, allowing the cognate ribosome binding site of the heterologous genes to be used. Techniques for overexpression in gram-positive species such as Bacillus are also known in the art and can be used in the context of this invention (Quax et al. In.: Industrial Microorganisms: Basic and Applied Molecular Genetics, Eds. Baltz et al., American Society for Microbiology, Washington (1993)). Alternate systems for overexpression rely for example, on yeast vectors and include the use of Pichia, Saccharomyces and Kluyveromyces (Sreekrishna, In: industrial microorganisms: basic and applied molecular genetics, Baltz, Hegeman, and Skatrud eds., American Society for Microbiology, Washington (1993); Dequin &amp; Barre, Biotechnology 12:173-177 (1994); van den Berg et al., Biotechnology 8:135-139 (1990)).  
         [0031]    Expression of the Nucleotide Sequences in Plant Tissue  
         [0032]    In a particularly preferred embodiment, at least one of the insecticidal toxins of the invention is expressed in a higher organism, e.g., a plant. In this case, transgenic plants expressing effective amounts of the toxins protect themselves from insect pests. When the insect starts feeding on such a transgenic plant, it also ingests the expressed toxins. This will deter the insect from further biting into the plant tissue or may even harm or kill the insect. A nucleotide sequence of the present invention is inserted into an expression cassette, which is then preferably stably integrated in the genome of said plant, In another preferred embodiment, the nucleotide sequence is included in a non-pathogenic self-replicating virus. Plants transformed in accordance with the present invention may be monocots or dicots and include, but are not limited to, maize, wheat, barley, rye, sweet potato, bean, pea, chicory, lettuce, cabbage, cauliflower, broccoli, turnip, radish, spinach, asparagus, onion, garlic, pepper, celery, squash, pumpkin, hemp, zucchini, apple, pear, quince, melon, plum, cherry, peach, nectarine, apricot, strawberry, grape, raspberry, blackberry, pineapple, avocado, papaya, mango, banana, soybean, tomato, sorghum, sugarcane, sugarbeet, sunflower, rapeseed, clover, tobacco, carrot, cotton, alfalfa, rice, potato, eggplant, cucumber, Arabidopsis, and woody plants such as coniferous and deciduous trees.  
         [0033]    Once a desired nucleotide sequence has been transformed into a particular plant species, it may be propagated in that species or moved into other varieties of the same species, particularly including commercial varieties, using traditional breeding techniques.  
         [0034]    A nucleotide sequence of this invention is preferably expressed in transgenic plants, thus causing the biosynthesis of the corresponding toxin in the transgenic plants. In this way, transgenic plants with enhanced resistance to insects are generated. For their expression in transgenic plants, the nucleotide sequences of the invention may require modification and optimization. Although in many cases genes from microbial organisms can be expressed in plants at high levels without modification, low expression in transgenic plants may result from microbial nucleotide sequences having codons that are not preferred in plants. It is known in the art that all organisms have specific preferences for codon usage, and the codons of the nucleotide sequences described in this invention can be changed to conform with plant preferences, while maintaining the amino acids encoded thereby. Furthermore, high expression in plants is best achieved from coding sequences that have at least about 35% GC content, preferably more than about 45%, more preferably more than about 50%, and most preferably more than about 60%. Microbial nucleotide sequences which have low GC contents may express poorly in plants due to the existence of ATTTA motifs which may destabilize messages, and AATAAA motifs which may cause inappropriate polyadenylation. Although preferred gene sequences may be adequately expressed in both monocotyledonous and dicotyledonous plant species, sequences can be modified to account for the specific codon preferences and GC content preferences of monocotyledons or dicotyledons as these preferences have been shown to differ (Murray et al. Nucl. Acids Res. 17: 477-498 (1989)). In addition, the nucleotide sequences are screened for the existence of illegitimate splice sites that may cause message truncation. All changes required to be made within the nucleotide sequences such as those described above are made using well known techniques of site directed mutagenesis, PCR, and synthetic gene construction.  
         [0035]    For efficient initiation of translation, sequences adjacent to the initiating methionine may require modification. For example, they can be modified by the inclusion of sequences known to be effective in plants. Joshi has suggested an appropriate consensus for plants (NAR 15: 6643-6653 (1987)) and Clontech suggests a further consensus translation initiator (1993/1994 catalog, page 210). These consensuses are suitable for use with the nucleotide sequences of this invention. The sequences are incorporated into constructions comprising the nucleotide sequences, up to and including the ATG (whilst leaving the second amino acid unmodified), or alternatively up to and including the GTC subsequent to the ATG (with the possibility of modifying the second amino acid of the transgene).  
         [0036]    Expression of the nucleotide sequences in transgenic plants is driven by promoters shown to be functional in plants. The choice of promoter will vary depending on the temporal and spatial requirements for expression, and also depending on the target species. Thus, expression of the nucleotide sequences of this invention in leaves, in ears, in inflorescences (e.g. spikes, panicles, cobs, etc.), in roots, and/or seedlings is preferred. In many cases, however, protection against more than one type of insect pest is sought, and thus expression in multiple tissues is desirable. Although many promoters from dicotyledons have been shown to be operational in monocotyledons and vice versa, ideally dicotyledonous promoters are selected for expression in dicotyledons, and monocotyledonous promoters for expression in monocotyledons. However, there is no restriction to the provenance of selected promoters; it is sufficient that they are operational in driving the expression of the nucleotide sequences in the desired cell.  
         [0037]    Preferred promoters that are expressed constitutively include promoters from genes encoding actin or ubiquitin and the CAMV 35S and 19S promoters. The nucleotide sequences of this invention can also be expressed under the regulation of promoters that are chemically regulated. This enables the insecticidal toxins to be synthesized only when the crop plants are treated with the inducing chemicals.  
         [0038]    A preferred category of promoters is that which is wound inducible. Numerous promoters have been described which are expressed at wound sites and also at the sites of phytopathogen infection. Ideally, such a promoter should only be active locally at the sites of infection, and in this way the insecticidal toxins only accumulate in cells which need to synthesize the insecticidal toxins to kill the invading insect pest. Preferred promoters of this kind include those described by Stanford et al. Mol. Gen. Genet. 215: 200-208 (1989), Xu et al. Plant Molec. Biol. 22: 573-588 (1993), Logemann et al. Plant Cell 1: 151-158 (1989), Rohrmeier &amp; Lehle, Plant Molec. Biol. 22: 783-792 (1993), Firek et al. Plant Molec. Biol. 22: 129-142 (1993), and Warner et al. Plant J. 3: 191-201 (1993).  
         [0039]    Especially preferred embodiments of the invention are transgenic plants expressing at least one of the nucleotide sequences of the invention in a root-preferred or root-specific fashion. Further preferred embodiments are transgenic plants expressing the nucleotide sequences in a wound-inducible or pathogen infection-inducible manner.  
         [0040]    In addition to the selection of a suitable promoter, constructions for expression of an insecticidal toxin in plants require an appropriate transcription terminator to be attached downstream of the heterologous nucleotide sequence. Several such terminators are available and known in the art (e.g. tml from Agrobacterium, E9 from rbcs). Any available terminator known to function in plants can be used in the context of this invention.  
         [0041]    Numerous other sequences can be incorporated into expression cassettes described in this invention. These include sequences which have been shown to enhance expression such as intron sequences (e.g. from Adhl and bronzel) and viral leader sequences (e.g. from TMV, MCMV and AMV).  
         [0042]    It may be preferable to target expression of the nucleotide sequences of the present invention to different cellular localizations in the plant. In some cases, localization in the cytosol may be desirable, whereas in other cases, localization in some subcellular organelle may be preferred. Subcellular localization of transgene encoded enzymes is undertaken using techniques well known in the art Typically, the DNA encoding the target peptide from a known organelle-targeted gene product is manipulated and fused upstream of the nucleotide sequence. Many such target sequences are known for the chloroplast and their functioning in heterologous constructions has been shown. The expression of the nucleotide sequences of the present invention is also targeted to the endoplasmic reticulum or to the vacuoles of the host cells. Techniques to achieve this are well-known in the art.  
         [0043]    Vectors suitable for plant transformation are described elsewhere in this specification. For Agrobacterium-mediated transformation, binary vectors or vectors carrying at least one T-DNA border sequence are suitable, whereas for direct gene transfer any vector is suitable and linear DNA containing only the construction of interest may be preferred. In the case of direct gene transfer, transformation with a single DNA species or cotransformation transformation can be used (Schocher et al. Biotechnology 4: 1093-1096 (1986)). For both direct gene transfer and Agrobacterium-mediated transfer, transformation is usually (but not necessarily) undertaken with a selectable or screenable marker which may provide resistance to an antibiotic (kanamycin, hygromycin or methotrexate) or a herbicide (Basta). Examples of such markers are neomycin phosphotransferase, hygromycin phosphotransferase, dihydrofolate reductase, phosphinothricin acetyltransferase, 2,2-dichloroproprionic acid dehalogenase, acetohydroxyacid synthase, 5-enolpyruvyl-shikimate-phosphate synthase, haloarylnitrilase, protoporhyrinogen oxidase, acetyl-coenzyme A carboxylase, dihydropteroate synthase, chloramphenicol acetyl transferase, and.-glucuronidase. The choice of selectable or screenable marker for plant transformation is not, however, critical to the invention.  
         [0044]    The recombinant DNA described above can be introduced into the plant cell in a number of artrecognized recognized ways. Those skilled in the art will appreciate that the choice of method might depend on the type of plant targeted for transformation. Suitable methods of transforming plant cells include microinjection (Crossway et al., BioTechniques 4.,320-334 (1986)), electroporation (Riggs et al., Proc. Natl. Acad, Sci. USA 83.,5602-5606 (1986), Agrobacterium-mediated transformation (Hinchee et al., Biotechnology 6:915-921 (1988); See also, lshida et al., Nature Biotechnology 14:745-750 (June 1996) (for maize transformation), direct gene transfer (Paszkowski et al., EMBO J. 3.2717-2722 (1984); Hayashimoto et al., Plant Physiol 93.857-863 (1990)(rice), and ballistic particle acceleration using devices available from Agracetus, Inc., Madison, Wis. and Dupont, Inc., Wilmington, Del. (see, for example, Sanford et al., U.S. Pat. No. 4,945,050; and McCabe et al., Biotechnology 6.923-926 (1988)). See also, Weissinger et al., Annual Rev Genet. 22.-421-477 (1988); Sanford et al., Particulate Science and Technology 5.27-37 (1987)(onion); Svab et al., Proc. Natl. Acad. Sci. USA 87.- 8526-8530 (1990) (tobacco chloroplast); Christou et al., Plant Physiol 87,671-674 (1988)(soybean); McCabe et al., BioTechnology 6.923-926 (1988)(soybean); Klein et al., Proc. Natl. Acad. Sci. USA, 85:4305-4309 (1988)(maize); Klein et al., BioTechnology 6.,559-563 (1988) (maize); Klein et al., Plant PhysioL 91.,440-444 (1988) (maize); Fromm et al., BioTechnology 8:833-839 (1990); and Gordon-Kamm et al., Plant Cell 2: 603-618 (1990) (maize); Koziel et al., Biotechnology 11: 194-200 (1993) (maize); Shimamoto et al., Nature 338: 274-277 (1989) (rice); Christou et al., Biotechnology 9: 957-962 (1991) (rice); Datta et al., BioTechnology 8.736-740 (1990) (rice); European Patent Application EP 0 332 581 (orchardgrass and other Pooideae); Vasil et al., Biotechnology 11: 1553-1558 (1993) (wheat); Weeks et al., Plant Physiol. 102:1077-1084 (1993) (wheat); Wan et al., Plant Physiol. 104:37-48 (1994) (barley); Jahne et al., Theor. Appl. Genet. 89:525-533 (1994)(barley); Umbeck et al., BioTechnology 5:263-266 (1987) (cotton); Casas et al., Proc. Natl. Acad. Sci. USA 90:11212-11216 (Dec. 1993) (sorghum); Somers et al., BioTechnology 10:1 589-1594 (Dec. 1992) (oat); Torbert et al., Plant Cell Reports 14:635-640 (1995) (oat); Weeks et al., Plant Physiol. 102:1077-1084 (1993) (wheat); Chang et al., WO 94/13822 (wheat) and Nehra et al., The Plant Journal 5:285-297 (1994) (wheat). A particularly preferred set of embodiments for the introduction of recombinant DNA molecules into maize by microprojectile bombardment can be found in Koziel et al., Biotechnology 11: 194-200(1993), Hill et al., Euphytica 85:119-123 (1995) and Koziel et al., Annals of the New York Academy of Sciences 792:164-171 (1996). An additional preferred embodiment is the protoplast transformation method for maize as disclosed in EP 0 292 435. Transformation of plants can be undertaken with a single DNA species or multiple DNA species (i.e. co-transformation).  
         [0045]    In another preferred embodiment, a nucleotide sequence of the present invention is directly transformed into the plastid genome. A major advantage of plastid transformation is that plastids are generally capable of expressing bacterial genes without substantial modification, and plastids are capable of expressing multiple open reading frames under control of a single promoter. Plastid transformation technology is extensively described in U.S. Pat. Nos. 5,451,513, 5,545,817, and 5,545,818, in PCT application no. WO 95/16783, and in McBride et al. (1994) Proc. Natl. Acad. Sci. USA 91, 7301-7305. The basic technique for chloroplast transformation involves introducing regions of cloned plastid DNA flanking a selectable marker together with the gene of interest into a suitable target tissue, e.g., using biolistics or protoplast transformation (e.g., calcium chloride or PEG mediated transformation). The 1 to 1.5 kb flanking regions, termed targeting sequences, facilitate homologous recombination with the plastid genome and thus allow the replacement or modification of specific regions of the plastome. Initially, point mutations in the chloroplast 16S rRNA and rpsl2 genes conferring resistance to spectinomycin and/or streptomycin are utilized as selectable markers for transformation (Svab, Z., Hajdukiewicz, P., and Maliga, P. (1990) Proc. Nati. Acad. Sci. USA 87, 8526-8530; Staub, J. M., and Maliga, P. (1992) Plant Cell 4, 39-45). This resulted in stable homoplasmic transformants at a frequency of approximately one per 100 bombardments of target leaves. The presence of cloning sites between these markers allowed creation of a plastid targeting vector for introduction of foreign genes (Staub, J. M., and Maliga, P. (1993) EMBO J. 12, 601-606). Substantial increases in transformation frequency are obtained by replacement of the recessive RRNA or r-protein antibiotic resistance genes with a dominant selectable marker, the bacterial aada gene encoding the spectinomycin-detoxifying enzyme aminoglycoside-3′ adenyltransferase (Svab, Z., and Maliga, P. (1993) Proc. Natl. Acad. Sci. USA 90, 913-917). Previously, this marker had been used successfully for high-frequency transformation of the plastid genome of the green alga Chlamydomonas reinhardtii (Goldschmidt-Clermont, M. (1991) Nucl. Acids Res. 19: 4083-4089). Other selectable markers useful for plastid transformation are known in the art and encompassed within the scope of the invention. Typically, approximately 15-20 cell division cycles following transformation are required to reach a homoplastidic state. Plastid expression, in which genes are inserted by homologous recombination into all of the several thousand copies of the circular plastid genome present in each plant cell, takes advantage of the enormous copy number advantage over nuclear-expressed genes to permit expression levels that can readily exceed 10% of the total soluble plant protein. In a preferred embodiment, a nucleotide sequence of the present invention is inserted into a plastid targeting vector and transformed into the plastid genome of a desired plant host. Plants homoplastic for plastid genomes containing a nucleotide sequence of the present invention are obtained, and are preferentially capable of high expression of the nucleotide sequence.  
         [0046]    Provisional Patent Application Ser. No. US 60/191806 filed Mar. 24, 2000, is hereby incorporated by reference.  
     
       
       
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             7551  
             DNA  
             Photorhabdus luminescens  
           
            1 

atgaacgagt ctgtaaaaga gatacctgat gtattaaaaa gccagtgtgg ttttaattgt     60 

ctgacagata ttagccacag ctcttttaat gaatttcgcc agcaagtatc tgagcacctc    120 

tcctggtccg aaacacacga cttatatcat gatgcacaac aggcacaaaa ggataatcgc    180 

ctgtatgaag cgcgtattct caaacgcgcc aatccccaat tacaaaatgc ggtgcatctt    240 

gccattctcg ctcccaatgc tgaactgata ggctataaca atcaatttag cggtagagcc    300 

agtcaatatg ttgcgccggg taccgtttct tccatgttct cccccgccgc ttatttgact    360 

gaactttatc gtgaagcacg caatttacac gcaagtgact ccgtttatta tctggatacc    420 

cgccgcccag atctcaaatc aatggcgctc agtcagcaaa atatggatat agaattatcc    480 

acactctctt tgtccaatga gctgttattg gaaagcatta aaactgaatc taaactggaa    540 

aactatacta aagtgatgga aatgctctcc actttccgtc cttccggcgc aacgccttat    600 

catgatgctt atgaaaatgt gcgtgaagtt atccagctac aagatcctgg acttgagcaa    660 

ctcaatgcat caccggcaat tgccgggttg atgcatcaag cctccctatt gggtattaac    720 

gcttcaatct cgcctgagct atttaatatt ctgacggagg agattaccga aggtaatgct    780 

gaggaacttt ataagaaaaa ttttggtaat atcgaaccgg cctcattggc tatgccggaa    840 

taccttaaac gttattataa tttaagcgat gaagaactta gtcagtttat tggtaaagcc    900 

agcaattttg gtcaacagga atatagtaat aaccaactta ttactccggt agtcaacagc    960 

agtgatggca cggttaaggt atatcggatc acccgcgaat atacaaccaa tgcttatcaa   1020 

atggatgtgg agctatttcc cttcggtggt gagaattatc ggttagatta taaattcaaa   1080 

aatttttata atgcctctta tttatccatc aagttaaatg ataaaagaga acttgttcga   1140 

actgaaggcg ctcctcaagt caatatagaa tactccgcaa atatcacatt aaataccgct   1200 

gatatcagtc aaccttttga aattggcctg acacgagtac ttccttccgg ttcttgggca   1260 

tatgccgccg caaaatttac cgttgaagag tataaccaat actcttttct gctaaaactt   1320 

aacaaggcta ttcgtctatc acgtgcgaca gaattgtcac ccacgattct ggaaggcatt   1380 

gtgcgcagtg ttaatctaca actggatatc aacacagacg tattaggtaa agtttttctg   1440 

actaaatatt atatgcagcg ttatgctatt catgctgaaa ctgccctgat actatgcaac   1500 

gcgcctattt cacaacgttc atatgataat caacctagcc aatttgatcg cctgtttaat   1560 

acgccattac tgaacggaca atatttttct accggcgatg aggagattga tttaaattca   1620 

ggtagcaccg gcgattggcg aaaaaccata cttaagcgtg catttaatat tgatgatgtc   1680 

tcgctcttcc gcctgcttaa aattaccgac catgataata aagatggaaa aattaaaaat   1740 

aacctaaaga atctttccaa tttatatatt ggaaaattac tggcagatat tcatcaatta   1800 

accattgatg aactggattt attactgatt gccgtaggtg aaggaaaaac taatttatcc   1860 

gctatcagtg ataagcaatt ggctaccctg atcagaaaac tcaatactat taccagctgg   1920 

ctacatacac agaagtggag tgtattccag ctatttatca tgacctccac cagctataac   1980 

aaaacgctaa cgcctgaaat taagaatttg ctggataccg tctaccacgg tttacaaggt   2040 

tttgataaag acaaagcaga tttgctacat gtcatggcgc cctatattgc ggccaccttg   2100 

caattatcat cggaaaatgt cgcccactcg gtactccttt gggcagataa gttacagccc   2160 

ggcgacggcg caatgacagc agaaaaattc tgggactggt tgaatactaa gtatacgccg   2220 

ggttcatcgg aagccgtaga aacgcaggaa catatcgttc agtattgtca ggctctggca   2280 

caattggaaa tggtttacca ttccaccggc atcaacgaaa acgccttccg tctatttgtg   2340 

acaaaaccag agatgtttgg cgctgcaact ggagcagcgc ccgcgcatga tgccctttca   2400 

ctgattatgc tgacacgttt tgcggattgg gtgaacgcac taggcgaaaa agcgtcctcg   2460 

gtgctagcgg catttgaagc taactcgtta acggcagaac aactggctga tgccatgaat   2520 

cttgatgcta atttgctgtt gcaagccagt attcaagcac aaaatcatca acatcttccc   2580 

ccagtaactc cagaaaatgc gttctcctgt tggacatcta tcaatactat cctgcaatgg   2640 

gttaatgtcg cacaacaatt gaatgtcgcc ccacagggcg tttccgcttt ggtcgggctg   2700 

gattatattc aatcaatgaa agagacaccg acctatgccc agtgggaaaa cgcggcaggc   2760 

gtattaaccg ccgggttgaa ttcacaacag gctaatacat tacacgcttt tctggatgaa   2820 

tctcgcagtg ccgcattaag cacctactat atccgtcaag tcgccaaggc agcggcggct   2880 

attaaaagcc gtgatgactt gtatcaatac ttactgattg ataatcaggt ttctgcggca   2940 

ataaaaacca cccggatcgc cgaagccatt gccagtattc aactgtacgt caaccgggca   3000 

ttggaaaatg tggaagaaaa tgccaattcg ggggttatca gccgccaatt ctttatcgac   3060 

tgggacaaat acaataaacg ctacagcact tgggcgggtg tttctcaatt agtttactac   3120 

ccggaaaact atattgatcc gaccatgcgt atcggacaaa ccaaaatgat ggacgcatta   3180 

ctgcaatccg tcagccaaag ccaattaaac gccgataccg tcgaagatgc ctttatgtct   3240 

tatctgacat cgtttgaaca agtggctaat cttaaagtta ttagcgcata tcacgataat   3300 

attaataacg atcaagggct gacctatttt atcggactca gtgaaactga tgccggtgaa   3360 

tattattggc gcagtgtcga tcacagtaaa ttcaacgacg gtaaattcgc ggctaatgcc   3420 

tggagtgaat ggcataaaat tgattgtcca attaaccctt ataaaagcac tatccgtcca   3480 

gtgatatata aatcccgcct gtatctgctc tggttggaac aaaaggagat caccaaacag   3540 

acaggaaata gtaaagatgg ctatcaaact gaaacggatt atcgttatga actaaaattg   3600 

gcgcatatcc gctatgatgg cacttggaat acgccaatca cctttgatgt caataaaaaa   3660 

atatccgagc taaaactgga aaaaaataga gcgcccggac tctattgtgc cggttatcaa   3720 

ggtgaagata cgttgctggt gatgttttat aaccaacaag acacactaga tagttataaa   3780 

aacgcttcaa tgcaaggact atatatcttt gctgatatgg catccaaaga tatgacccca   3840 

gaacagagca atgtttatcg ggataatagc tatcaacaat ttgataccaa taatgtcaga   3900 

agagtgaata accgctatgc agaggattat gagattcctt cctcggtaag tagccgtaaa   3960 

gactatggtt ggggagatta ttacctcagc atggtatata acggagatat tccaactatc   4020 

aattacaaag ccgcatcaag tgatttaaaa atctatatct caccaaaatt aagaattatt   4080 

cataatggat atgaaggaca gaagcgcaat caatgcaatc tgatgaataa atatggcaaa   4140 

ctaggtgata aatttattgt ttatactagc ttgggggtca atccaaataa ctcgtcaaat   4200 

aagctcatgt tttaccccgt ctatcaatat agcggaaaca ccagtggact caatcaaggg   4260 

agactactat tccaccgtga caccacttat ccatctaaag tagaagcttg gattcctgga   4320 

gcaaaacgtt ctctaaccaa ccaaaatgcc gccattggtg atgattatgc tacagactct   4380 

ctgaataaac cggatgatct taagcaatat atctttatga ctgacagtaa agggactgct   4440 

actgatgtct caggcccagt agagattaat actgcaattt ctccagcaaa agttcagata   4500 

atagtcaaag cgggtggcaa ggagcaaact tttaccgcag ataaagatgt ctccattcag   4560 

ccatcaccta gctttgatga aatgaattat caatttaatg cccttgaaat agacggttct   4620 

ggtctgaatt ttattaacaa ctcagccagt attgatgtta cttttaccgc atttgcggag   4680 

gatggccgca aactgggtta tgaaagtttc agtattcctg ttaccctcaa ggtaagtacc   4740 

gataatgccc tgaccctgca ccataatgaa aatggtgcgc aatatatgca atggcaatcc   4800 

tatcgtaccc gcctgaatac tctatttgcc cgccagttgg ttgcacgcgc caccaccgga   4860 

atcgatacaa ttctgagtat ggaaactcag aatattcagg aaccgcagtt aggcaaaggt   4920 

ttctatgcta cgttcgtgat acctccctat aacctatcaa ctcatggtga tgaacgttgg   4980 

tttaagcttt atatcaaaca tgttgttgat aataattcac atattatcta ttcaggccag   5040 

ctaacagata caaatataaa catcacatta tttattcctc ttgatgatgt cccattgaat   5100 

caagattatc acgccaaggt ttatatgacc ttcaagaaat caccatcaga tggtacctgg   5160 

tggggccctc actttgttag agatgataaa ggaatagtaa caataaaccc taaatccatt   5220 

ttgacccatt ttgagagcgt caatgtcctg aataatatta gtagcgaacc aatggatttc   5280 

agcggcgcta acagcctcta tttctgggaa ctgttctact ataccccgat gctggttgct   5340 

caacgtttgc tgcatgaaca gaacttcgat gaagccaacc gttggctgaa atatgtctgg   5400 

agtccatccg gttatattgt ccacggccag attcagaact accagtggaa cgtccgcccg   5460 

ttactggaag acaccagttg gaacagtgat cctttggatt ccgtcgatcc tgacgcggta   5520 

gcacagcacg atccaatgca ctacaaagtt tcaactttta tgcgtacctt ggatctattg   5580 

atagcacgcg gcgaccatgc ttatcgccaa ctggaacgag atacactcaa cgaagcgaag   5640 

atgtggtata tgcaagcgct gcatctatta ggtgacaaac cttatctacc gctgagtacg   5700 

acatggagtg atccacgact agacagagcc gcggatatca ctacccaaaa tgctcacgac   5760 

agcgcaatag tcgctctgcg gcagaatata cctacaccgg cacctttatc attgcgcagc   5820 

gctaataccc tgactgatct cttcctgccg caaatcaatg aagtgatgat gaattactgg   5880 

cagacattag ctcagagagt atacaatctg cgtcataacc tctctatcga cggccagccg   5940 

ttatatctgc caatctatgc cacaccggcc gatccgaaag cgttactcag cgccgccgtt   6000 

gccacttctc aaggtggagg caagctaccg gaatcattta tgtccctgtg gcgtttcccg   6060 

cacatgctgg aaaatgcgcg cggcatggtt agccagctca cccagttcgg ctccacgtta   6120 

caaaatatta tcgaacgtca ggacgcggaa gcgctcaatg cgttattaca aaatcaggcc   6180 

gccgagctga tattgactaa cctgagcatt caggacaaaa ccattgaaga attggatgcc   6240 

gagaaaacgg tgttggaaaa atccaaagcg ggagcacaat cgcgctttga tagctacggc   6300 

aaactgtacg atgagaatat caacgccggt gaaaaccaag ccatgacgct acgagcgtcc   6360 

gccgccgggc ttaccacggc agttcaggca tcccgtctgg ccggtgcggc ggctgatctg   6420 

gtgcctaaca tcttcggctt tgccggtggc ggcagccgtt ggggggctat cgctgaggcg   6480 

acaggttatg tgatggaatt ctccgcgaat gttatgaaca ccgaagcgga taaaattagc   6540 

caatctgaaa cctaccgtcg tcgccgtcag gagtgggaga tccagcggaa taatgccgaa   6600 

gcggaattga agcaaatcga tgctcagctc aaatcactcg ctgtacgccg cgaagccgcc   6660 

gtattgcaga aaaccagtct gaaaacccaa caagaacaga cccaatctca attggccttc   6720 

ctgcaacgta agttcagcaa tcaggcgtta tacaactggc tgcgtggtcg actggcggcg   6780 

atttacttcc agttctacga tttggccgtc gcgcgttgcc tgatggcaga acaagcttac   6840 

cgttgggaac tcaatgatga ctctgcccgc ttcattaaac cgggcgcctg gcagggaacc   6900 

tatgccggtc tgcttgcagg tgaaaccttg atgctgagtc tggcacaaat ggaagacgct   6960 

catctgaaac gcgataaacg cgcattagag gttgaacgca cagtatcgct ggccgaagtt   7020 

tatgcaggat taccaaaaga taacggtcca ttttccctgg ctcaggaaat tgacaagctg   7080 

gtgagtcaag gttcaggcag tgccggcagt ggtaataata atttggcgtt cggcgccggc   7140 

acggacacta aaacctcttt gcaggcatca gtttcattcg ctgatttgaa aattcgtgaa   7200 

gattacccgg catcgcttgg caaaattcga cgtatcaaac agatcagcgt cactttgccc   7260 

gcgctactgg gaccgtatca ggatgtacag gcaatattgt cttacggcga taaagccgga   7320 

ttagctaacg gctgtgaagc gctggcagtt tctcacggta tgaatgacag cggccaattc   7380 

cagctcgatt tcaacgatgg caaattcctg ccattcgaag gcatcgccat tgatcaaggc   7440 

acgctgacac tgagcttccc aaatgcatct atgccggaga aaggtaaaca agccactatg   7500 

ttaaaaaccc tgaacgatat cattttgcat attcgctaca ccattaaata a            7551 

 
           
             2  
             2516  
             PRT  
             Photorhabdus luminescens  
           
            2 

Met Asn Glu Ser Val Lys Glu Ile Pro Asp Val Leu Lys Ser Gln Cys 
1               5                   10                  15 

Gly Phe Asn Cys Leu Thr Asp Ile Ser His Ser Ser Phe Asn Glu Phe 
            20                  25                  30 

Arg Gln Gln Val Ser Glu His Leu Ser Trp Ser Glu Thr His Asp Leu 
        35                  40                  45 

Tyr His Asp Ala Gln Gln Ala Gln Lys Asp Asn Arg Leu Tyr Glu Ala 
    50                  55                  60 

Arg Ile Leu Lys Arg Ala Asn Pro Gln Leu Gln Asn Ala Val His Leu 
65                  70                  75                  80 

Ala Ile Leu Ala Pro Asn Ala Glu Leu Ile Gly Tyr Asn Asn Gln Phe 
                85                  90                  95 

Ser Gly Arg Ala Ser Gln Tyr Val Ala Pro Gly Thr Val Ser Ser Met 
            100                 105                 110 

Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Arg Asn 
        115                 120                 125 

Leu His Ala Ser Asp Ser Val Tyr Tyr Leu Asp Thr Arg Arg Pro Asp 
    130                 135                 140 

Leu Lys Ser Met Ala Leu Ser Gln Gln Asn Met Asp Ile Glu Leu Ser 
145                 150                 155                 160 

Thr Leu Ser Leu Ser Asn Glu Leu Leu Leu Glu Ser Ile Lys Thr Glu 
                165                 170                 175 

Ser Lys Leu Glu Asn Tyr Thr Lys Val Met Glu Met Leu Ser Thr Phe 
            180                 185                 190 

Arg Pro Ser Gly Ala Thr Pro Tyr His Asp Ala Tyr Glu Asn Val Arg 
        195                 200                 205 

Glu Val Ile Gln Leu Gln Asp Pro Gly Leu Glu Gln Leu Asn Ala Ser 
    210                 215                 220 

Pro Ala Ile Ala Gly Leu Met His Gln Ala Ser Leu Leu Gly Ile Asn 
225                 230                 235                 240 

Ala Ser Ile Ser Pro Glu Leu Phe Asn Ile Leu Thr Glu Glu Ile Thr 
                245                 250                 255 

Glu Gly Asn Ala Glu Glu Leu Tyr Lys Lys Asn Phe Gly Asn Ile Glu 
            260                 265                 270 

Pro Ala Ser Leu Ala Met Pro Glu Tyr Leu Lys Arg Tyr Tyr Asn Leu 
        275                 280                 285 

Ser Asp Glu Glu Leu Ser Gln Phe Ile Gly Lys Ala Ser Asn Phe Gly 
    290                 295                 300 

Gln Gln Glu Tyr Ser Asn Asn Gln Leu Ile Thr Pro Val Val Asn Ser 
305                 310                 315                 320 

Ser Asp Gly Thr Val Lys Val Tyr Arg Ile Thr Arg Glu Tyr Thr Thr 
                325                 330                 335 

Asn Ala Tyr Gln Met Asp Val Glu Leu Phe Pro Phe Gly Gly Glu Asn 
            340                 345                 350 

Tyr Arg Leu Asp Tyr Lys Phe Lys Asn Phe Tyr Asn Ala Ser Tyr Leu 
        355                 360                 365 

Ser Ile Lys Leu Asn Asp Lys Arg Glu Leu Val Arg Thr Glu Gly Ala 
    370                 375                 380 

Pro Gln Val Asn Ile Glu Tyr Ser Ala Asn Ile Thr Leu Asn Thr Ala 
385                 390                 395                 400 

Asp Ile Ser Gln Pro Phe Glu Ile Gly Leu Thr Arg Val Leu Pro Ser 
                405                 410                 415 

Gly Ser Trp Ala Tyr Ala Ala Ala Lys Phe Thr Val Glu Glu Tyr Asn 
            420                 425                 430 

Gln Tyr Ser Phe Leu Leu Lys Leu Asn Lys Ala Ile Arg Leu Ser Arg 
        435                 440                 445 

Ala Thr Glu Leu Ser Pro Thr Ile Leu Glu Gly Ile Val Arg Ser Val 
    450                 455                 460 

Asn Leu Gln Leu Asp Ile Asn Thr Asp Val Leu Gly Lys Val Phe Leu 
465                 470                 475                 480 

Thr Lys Tyr Tyr Met Gln Arg Tyr Ala Ile His Ala Glu Thr Ala Leu 
                485                 490                 495 

Ile Leu Cys Asn Ala Pro Ile Ser Gln Arg Ser Tyr Asp Asn Gln Pro 
            500                 505                 510 

Ser Gln Phe Asp Arg Leu Phe Asn Thr Pro Leu Leu Asn Gly Gln Tyr 
        515                 520                 525 

Phe Ser Thr Gly Asp Glu Glu Ile Asp Leu Asn Ser Gly Ser Thr Gly 
    530                 535                 540 

Asp Trp Arg Lys Thr Ile Leu Lys Arg Ala Phe Asn Ile Asp Asp Val 
545                 550                 555                 560 

Ser Leu Phe Arg Leu Leu Lys Ile Thr Asp His Asp Asn Lys Asp Gly 
                565                 570                 575 

Lys Ile Lys Asn Asn Leu Lys Asn Leu Ser Asn Leu Tyr Ile Gly Lys 
            580                 585                 590 

Leu Leu Ala Asp Ile His Gln Leu Thr Ile Asp Glu Leu Asp Leu Leu 
        595                 600                 605 

Leu Ile Ala Val Gly Glu Gly Lys Thr Asn Leu Ser Ala Ile Ser Asp 
    610                 615                 620 

Lys Gln Leu Ala Thr Leu Ile Arg Lys Leu Asn Thr Ile Thr Ser Trp 
625                 630                 635                 640 

Leu His Thr Gln Lys Trp Ser Val Phe Gln Leu Phe Ile Met Thr Ser 
                645                 650                 655 

Thr Ser Tyr Asn Lys Thr Leu Thr Pro Glu Ile Lys Asn Leu Leu Asp 
            660                 665                 670 

Thr Val Tyr His Gly Leu Gln Gly Phe Asp Lys Asp Lys Ala Asp Leu 
        675                 680                 685 

Leu His Val Met Ala Pro Tyr Ile Ala Ala Thr Leu Gln Leu Ser Ser 
    690                 695                 700 

Glu Asn Val Ala His Ser Val Leu Leu Trp Ala Asp Lys Leu Gln Pro 
705                 710                 715                 720 

Gly Asp Gly Ala Met Thr Ala Glu Lys Phe Trp Asp Trp Leu Asn Thr 
                725                 730                 735 

Lys Tyr Thr Pro Gly Ser Ser Glu Ala Val Glu Thr Gln Glu His Ile 
            740                 745                 750 

Val Gln Tyr Cys Gln Ala Leu Ala Gln Leu Glu Met Val Tyr His Ser 
        755                 760                 765 

Thr Gly Ile Asn Glu Asn Ala Phe Arg Leu Phe Val Thr Lys Pro Glu 
    770                 775                 780 

Met Phe Gly Ala Ala Thr Gly Ala Ala Pro Ala His Asp Ala Leu Ser 
785                 790                 795                 800 

Leu Ile Met Leu Thr Arg Phe Ala Asp Trp Val Asn Ala Leu Gly Glu 
                805                 810                 815 

Lys Ala Ser Ser Val Leu Ala Ala Phe Glu Ala Asn Ser Leu Thr Ala 
            820                 825                 830 

Glu Gln Leu Ala Asp Ala Met Asn Leu Asp Ala Asn Leu Leu Leu Gln 
        835                 840                 845 

Ala Ser Ile Gln Ala Gln Asn His Gln His Leu Pro Pro Val Thr Pro 
    850                 855                 860 

Glu Asn Ala Phe Ser Cys Trp Thr Ser Ile Asn Thr Ile Leu Gln Trp 
865                 870                 875                 880 

Val Asn Val Ala Gln Gln Leu Asn Val Ala Pro Gln Gly Val Ser Ala 
                885                 890                 895 

Leu Val Gly Leu Asp Tyr Ile Gln Ser Met Lys Glu Thr Pro Thr Tyr 
            900                 905                 910 

Ala Gln Trp Glu Asn Ala Ala Gly Val Leu Thr Ala Gly Leu Asn Ser 
        915                 920                 925 

Gln Gln Ala Asn Thr Leu His Ala Phe Leu Asp Glu Ser Arg Ser Ala 
    930                 935                 940 

Ala Leu Ser Thr Tyr Tyr Ile Arg Gln Val Ala Lys Ala Ala Ala Ala 
945                 950                 955                 960 

Ile Lys Ser Arg Asp Asp Leu Tyr Gln Tyr Leu Leu Ile Asp Asn Gln 
                965                 970                 975 

Val Ser Ala Ala Ile Lys Thr Thr Arg Ile Ala Glu Ala Ile Ala Ser 
            980                 985                 990 

Ile Gln Leu Tyr Val Asn Arg Ala  Leu Glu Asn Val Glu  Glu Asn Ala 
        995                 1000                 1005 

Asn Ser  Gly Val Ile Ser Arg  Gln Phe Phe Ile Asp  Trp Asp Lys 
    1010                 1015                 1020 

Tyr Asn  Lys Arg Tyr Ser Thr  Trp Ala Gly Val Ser  Gln Leu Val 
    1025                 1030                 1035 

Tyr Tyr  Pro Glu Asn Tyr Ile  Asp Pro Thr Met Arg  Ile Gly Gln 
    1040                 1045                 1050 

Thr Lys  Met Met Asp Ala Leu  Leu Gln Ser Val Ser  Gln Ser Gln 
    1055                 1060                 1065 

Leu Asn  Ala Asp Thr Val Glu  Asp Ala Phe Met Ser  Tyr Leu Thr 
    1070                 1075                 1080 

Ser Phe  Glu Gln Val Ala Asn  Leu Lys Val Ile Ser  Ala Tyr His 
    1085                 1090                 1095 

Asp Asn  Ile Asn Asn Asp Gln  Gly Leu Thr Tyr Phe  Ile Gly Leu 
    1100                 1105                 1110 

Ser Glu  Thr Asp Ala Gly Glu  Tyr Tyr Trp Arg Ser  Val Asp His 
    1115                 1120                 1125 

Ser Lys  Phe Asn Asp Gly Lys  Phe Ala Ala Asn Ala  Trp Ser Glu 
    1130                 1135                 1140 

Trp His  Lys Ile Asp Cys Pro  Ile Asn Pro Tyr Lys  Ser Thr Ile 
    1145                 1150                 1155 

Arg Pro  Val Ile Tyr Lys Ser  Arg Leu Tyr Leu Leu  Trp Leu Glu 
    1160                 1165                 1170 

Gln Lys  Glu Ile Thr Lys Gln  Thr Gly Asn Ser Lys  Asp Gly Tyr 
    1175                 1180                 1185 

Gln Thr  Glu Thr Asp Tyr Arg  Tyr Glu Leu Lys Leu  Ala His Ile 
    1190                 1195                 1200 

Arg Tyr  Asp Gly Thr Trp Asn  Thr Pro Ile Thr Phe  Asp Val Asn 
    1205                 1210                 1215 

Lys Lys  Ile Ser Glu Leu Lys  Leu Glu Lys Asn Arg  Ala Pro Gly 
    1220                 1225                 1230 

Leu Tyr  Cys Ala Gly Tyr Gln  Gly Glu Asp Thr Leu  Leu Val Met 
    1235                 1240                 1245 

Phe Tyr  Asn Gln Gln Asp Thr  Leu Asp Ser Tyr Lys  Asn Ala Ser 
    1250                 1255                 1260 

Met Gln  Gly Leu Tyr Ile Phe  Ala Asp Met Ala Ser  Lys Asp Met 
    1265                 1270                 1275 

Thr Pro  Glu Gln Ser Asn Val  Tyr Arg Asp Asn Ser  Tyr Gln Gln 
    1280                 1285                 1290 

Phe Asp  Thr Asn Asn Val Arg  Arg Val Asn Asn Arg  Tyr Ala Glu 
    1295                 1300                 1305 

Asp Tyr  Glu Ile Pro Ser Ser  Val Ser Ser Arg Lys  Asp Tyr Gly 
    1310                 1315                 1320 

Trp Gly  Asp Tyr Tyr Leu Ser  Met Val Tyr Asn Gly  Asp Ile Pro 
    1325                 1330                 1335 

Thr Ile  Asn Tyr Lys Ala Ala  Ser Ser Asp Leu Lys  Ile Tyr Ile 
    1340                 1345                 1350 

Ser Pro  Lys Leu Arg Ile Ile  His Asn Gly Tyr Glu  Gly Gln Lys 
    1355                 1360                 1365 

Arg Asn  Gln Cys Asn Leu Met  Asn Lys Tyr Gly Lys  Leu Gly Asp 
    1370                 1375                 1380 

Lys Phe  Ile Val Tyr Thr Ser  Leu Gly Val Asn Pro  Asn Asn Ser 
    1385                 1390                 1395 

Ser Asn  Lys Leu Met Phe Tyr  Pro Val Tyr Gln Tyr  Ser Gly Asn 
    1400                 1405                 1410 

Thr Ser  Gly Leu Asn Gln Gly  Arg Leu Leu Phe His  Arg Asp Thr 
    1415                 1420                 1425 

Thr Tyr  Pro Ser Lys Val Glu  Ala Trp Ile Pro Gly  Ala Lys Arg 
    1430                 1435                 1440 

Ser Leu  Thr Asn Gln Asn Ala  Ala Ile Gly Asp Asp  Tyr Ala Thr 
    1445                 1450                 1455 

Asp Ser  Leu Asn Lys Pro Asp  Asp Leu Lys Gln Tyr  Ile Phe Met 
    1460                 1465                 1470 

Thr Asp  Ser Lys Gly Thr Ala  Thr Asp Val Ser Gly  Pro Val Glu 
    1475                 1480                 1485 

Ile Asn  Thr Ala Ile Ser Pro  Ala Lys Val Gln Ile  Ile Val Lys 
    1490                 1495                 1500 

Ala Gly  Gly Lys Glu Gln Thr  Phe Thr Ala Asp Lys  Asp Val Ser 
    1505                 1510                 1515 

Ile Gln  Pro Ser Pro Ser Phe  Asp Glu Met Asn Tyr  Gln Phe Asn 
    1520                 1525                 1530 

Ala Leu  Glu Ile Asp Gly Ser  Gly Leu Asn Phe Ile  Asn Asn Ser 
    1535                 1540                 1545 

Ala Ser  Ile Asp Val Thr Phe  Thr Ala Phe Ala Glu  Asp Gly Arg 
    1550                 1555                 1560 

Lys Leu  Gly Tyr Glu Ser Phe  Ser Ile Pro Val Thr  Leu Lys Val 
    1565                 1570                 1575 

Ser Thr  Asp Asn Ala Leu Thr  Leu His His Asn Glu  Asn Gly Ala 
    1580                 1585                 1590 

Gln Tyr  Met Gln Trp Gln Ser  Tyr Arg Thr Arg Leu  Asn Thr Leu 
    1595                 1600                 1605 

Phe Ala  Arg Gln Leu Val Ala  Arg Ala Thr Thr Gly  Ile Asp Thr 
    1610                 1615                 1620 

Ile Leu  Ser Met Glu Thr Gln  Asn Ile Gln Glu Pro  Gln Leu Gly 
    1625                 1630                 1635 

Lys Gly  Phe Tyr Ala Thr Phe  Val Ile Pro Pro Tyr  Asn Leu Ser 
    1640                 1645                 1650 

Thr His  Gly Asp Glu Arg Trp  Phe Lys Leu Tyr Ile  Lys His Val 
    1655                 1660                 1665 

Val Asp  Asn Asn Ser His Ile  Ile Tyr Ser Gly Gln  Leu Thr Asp 
    1670                 1675                 1680 

Thr Asn  Ile Asn Ile Thr Leu  Phe Ile Pro Leu Asp  Asp Val Pro 
    1685                 1690                 1695 

Leu Asn  Gln Asp Tyr His Ala  Lys Val Tyr Met Thr  Phe Lys Lys 
    1700                 1705                 1710 

Ser Pro  Ser Asp Gly Thr Trp  Trp Gly Pro His Phe  Val Arg Asp 
    1715                 1720                 1725 

Asp Lys  Gly Ile Val Thr Ile  Asn Pro Lys Ser Ile  Leu Thr His 
    1730                 1735                 1740 

Phe Glu  Ser Val Asn Val Leu  Asn Asn Ile Ser Ser  Glu Pro Met 
    1745                 1750                 1755 

Asp Phe  Ser Gly Ala Asn Ser  Leu Tyr Phe Trp Glu  Leu Phe Tyr 
    1760                 1765                 1770 

Tyr Thr  Pro Met Leu Val Ala  Gln Arg Leu Leu His  Glu Gln Asn 
    1775                 1780                 1785 

Phe Asp  Glu Ala Asn Arg Trp  Leu Lys Tyr Val Trp  Ser Pro Ser 
    1790                 1795                 1800 

Gly Tyr  Ile Val His Gly Gln  Ile Gln Asn Tyr Gln  Trp Asn Val 
    1805                 1810                 1815 

Arg Pro  Leu Leu Glu Asp Thr  Ser Trp Asn Ser Asp  Pro Leu Asp 
    1820                 1825                 1830 

Ser Val  Asp Pro Asp Ala Val  Ala Gln His Asp Pro  Met His Tyr 
    1835                 1840                 1845 

Lys Val  Ser Thr Phe Met Arg  Thr Leu Asp Leu Leu  Ile Ala Arg 
    1850                 1855                 1860 

Gly Asp  His Ala Tyr Arg Gln  Leu Glu Arg Asp Thr  Leu Asn Glu 
    1865                 1870                 1875 

Ala Lys  Met Trp Tyr Met Gln  Ala Leu His Leu Leu  Gly Asp Lys 
    1880                 1885                 1890 

Pro Tyr  Leu Pro Leu Ser Thr  Thr Trp Ser Asp Pro  Arg Leu Asp 
    1895                 1900                 1905 

Arg Ala  Ala Asp Ile Thr Thr  Gln Asn Ala His Asp  Ser Ala Ile 
    1910                 1915                 1920 

Val Ala  Leu Arg Gln Asn Ile  Pro Thr Pro Ala Pro  Leu Ser Leu 
    1925                 1930                 1935 

Arg Ser  Ala Asn Thr Leu Thr  Asp Leu Phe Leu Pro  Gln Ile Asn 
    1940                 1945                 1950 

Glu Val  Met Met Asn Tyr Trp  Gln Thr Leu Ala Gln  Arg Val Tyr 
    1955                 1960                 1965 

Asn Leu  Arg His Asn Leu Ser  Ile Asp Gly Gln Pro  Leu Tyr Leu 
    1970                 1975                 1980 

Pro Ile  Tyr Ala Thr Pro Ala  Asp Pro Lys Ala Leu  Leu Ser Ala 
    1985                 1990                 1995 

Ala Val  Ala Thr Ser Gln Gly  Gly Gly Lys Leu Pro  Glu Ser Phe 
    2000                 2005                 2010 

Met Ser  Leu Trp Arg Phe Pro  His Met Leu Glu Asn  Ala Arg Gly 
    2015                 2020                 2025 

Met Val  Ser Gln Leu Thr Gln  Phe Gly Ser Thr Leu  Gln Asn Ile 
    2030                 2035                 2040 

Ile Glu  Arg Gln Asp Ala Glu  Ala Leu Asn Ala Leu  Leu Gln Asn 
    2045                 2050                 2055 

Gln Ala  Ala Glu Leu Ile Leu  Thr Asn Leu Ser Ile  Gln Asp Lys 
    2060                 2065                 2070 

Thr Ile  Glu Glu Leu Asp Ala  Glu Lys Thr Val Leu  Glu Lys Ser 
    2075                 2080                 2085 

Lys Ala  Gly Ala Gln Ser Arg  Phe Asp Ser Tyr Gly  Lys Leu Tyr 
    2090                 2095                 2100 

Asp Glu  Asn Ile Asn Ala Gly  Glu Asn Gln Ala Met  Thr Leu Arg 
    2105                 2110                 2115 

Ala Ser  Ala Ala Gly Leu Thr  Thr Ala Val Gln Ala  Ser Arg Leu 
    2120                 2125                 2130 

Ala Gly  Ala Ala Ala Asp Leu  Val Pro Asn Ile Phe  Gly Phe Ala 
    2135                 2140                 2145 

Gly Gly  Gly Ser Arg Trp Gly  Ala Ile Ala Glu Ala  Thr Gly Tyr 
    2150                 2155                 2160 

Val Met  Glu Phe Ser Ala Asn  Val Met Asn Thr Glu  Ala Asp Lys 
    2165                 2170                 2175 

Ile Ser  Gln Ser Glu Thr Tyr  Arg Arg Arg Arg Gln  Glu Trp Glu 
    2180                 2185                 2190 

Ile Gln  Arg Asn Asn Ala Glu  Ala Glu Leu Lys Gln  Ile Asp Ala 
    2195                 2200                 2205 

Gln Leu  Lys Ser Leu Ala Val  Arg Arg Glu Ala Ala  Val Leu Gln 
    2210                 2215                 2220 

Lys Thr  Ser Leu Lys Thr Gln  Gln Glu Gln Thr Gln  Ser Gln Leu 
    2225                 2230                 2235 

Ala Phe  Leu Gln Arg Lys Phe  Ser Asn Gln Ala Leu  Tyr Asn Trp 
    2240                 2245                 2250 

Leu Arg  Gly Arg Leu Ala Ala  Ile Tyr Phe Gln Phe  Tyr Asp Leu 
    2255                 2260                 2265 

Ala Val  Ala Arg Cys Leu Met  Ala Glu Gln Ala Tyr  Arg Trp Glu 
    2270                 2275                 2280 

Leu Asn  Asp Asp Ser Ala Arg  Phe Ile Lys Pro Gly  Ala Trp Gln 
    2285                 2290                 2295 

Gly Thr  Tyr Ala Gly Leu Leu  Ala Gly Glu Thr Leu  Met Leu Ser 
    2300                 2305                 2310 

Leu Ala  Gln Met Glu Asp Ala  His Leu Lys Arg Asp  Lys Arg Ala 
    2315                 2320                 2325 

Leu Glu  Val Glu Arg Thr Val  Ser Leu Ala Glu Val  Tyr Ala Gly 
    2330                 2335                 2340 

Leu Pro  Lys Asp Asn Gly Pro  Phe Ser Leu Ala Gln  Glu Ile Asp 
    2345                 2350                 2355 

Lys Leu  Val Ser Gln Gly Ser  Gly Ser Ala Gly Ser  Gly Asn Asn 
    2360                 2365                 2370 

Asn Leu  Ala Phe Gly Ala Gly  Thr Asp Thr Lys Thr  Ser Leu Gln 
    2375                 2380                 2385 

Ala Ser  Val Ser Phe Ala Asp  Leu Lys Ile Arg Glu  Asp Tyr Pro 
    2390                 2395                 2400 

Ala Ser  Leu Gly Lys Ile Arg  Arg Ile Lys Gln Ile  Ser Val Thr 
    2405                 2410                 2415 

Leu Pro  Ala Leu Leu Gly Pro  Tyr Gln Asp Val Gln  Ala Ile Leu 
    2420                 2425                 2430 

Ser Tyr  Gly Asp Lys Ala Gly  Leu Ala Asn Gly Cys  Glu Ala Leu 
    2435                 2440                 2445 

Ala Val  Ser His Gly Met Asn  Asp Ser Gly Gln Phe  Gln Leu Asp 
    2450                 2455                 2460 

Phe Asn  Asp Gly Lys Phe Leu  Pro Phe Glu Gly Ile  Ala Ile Asp 
    2465                 2470                 2475 

Gln Gly  Thr Leu Thr Leu Ser  Phe Pro Asn Ala Ser  Met Pro Glu 
    2480                 2485                 2490 

Lys Gly  Lys Gln Ala Thr Met  Leu Lys Thr Leu Asn  Asp Ile Ile 
    2495                 2500                 2505 

Leu His  Ile Arg Tyr Thr Ile  Lys 
    2510                 2515 

 
           
             3  
             4431  
             DNA  
             Photorhabdus luminescens  
             
               CDS  
               (1)..(4431)  
             
           
            3 

atg cag aat tca caa aca ttc agt gtt acc gag ctg tca tta ccc aaa       48 
Met Gln Asn Ser Gln Thr Phe Ser Val Thr Glu Leu Ser Leu Pro Lys 
1               5                   10                  15 

ggc ggc ggc gct att acc ggt atg ggt gaa gca tta aca cca gcc ggg       96 
Gly Gly Gly Ala Ile Thr Gly Met Gly Glu Ala Leu Thr Pro Ala Gly 
            20                  25                  30 

ccg gat ggt atg gcc gcc tta tcc ctg cca tta ccc att tcc gcc ggg      144 
Pro Asp Gly Met Ala Ala Leu Ser Leu Pro Leu Pro Ile Ser Ala Gly 
        35                  40                  45 

cgt ggt tac gca ccc tcg ctc act ctg aat tac aac agt gga acc ggt      192 
Arg Gly Tyr Ala Pro Ser Leu Thr Leu Asn Tyr Asn Ser Gly Thr Gly 
    50                  55                  60 

aac agc cca ttt ggt ctc ggt tgg gac tgc ggc gtc atg gca att cgt      240 
Asn Ser Pro Phe Gly Leu Gly Trp Asp Cys Gly Val Met Ala Ile Arg 
65                  70                  75                  80 

cgt cgc acc agt acc ggc gta ccg aat tac gat gaa acc gat act ttt      288 
Arg Arg Thr Ser Thr Gly Val Pro Asn Tyr Asp Glu Thr Asp Thr Phe 
                85                  90                  95 

ctg ggg ccg gaa ggt gaa gtg ttg gtc gta gca tta aat gag gca ggt      336 
Leu Gly Pro Glu Gly Glu Val Leu Val Val Ala Leu Asn Glu Ala Gly 
            100                 105                 110 

caa gct gat atc cgc agt gaa tcc tca ttg cag ggc atc aat ttg ggt      384 
Gln Ala Asp Ile Arg Ser Glu Ser Ser Leu Gln Gly Ile Asn Leu Gly 
        115                 120                 125 

gcg acc ttc acc gtt acc tgt tat cgc tcc cgc cta gaa agc cac ttt      432 
Ala Thr Phe Thr Val Thr Cys Tyr Arg Ser Arg Leu Glu Ser His Phe 
    130                 135                 140 

aac cgg ttg gaa tac tgg caa ccc caa aca acc ggc gca acc gat ttc      480 
Asn Arg Leu Glu Tyr Trp Gln Pro Gln Thr Thr Gly Ala Thr Asp Phe 
145                 150                 155                 160 

tgg ctg ata tac agc ccc gac gga cag gtc cat tta ctg ggc aaa aat      528 
Trp Leu Ile Tyr Ser Pro Asp Gly Gln Val His Leu Leu Gly Lys Asn 
                165                 170                 175 

cct cag gca cgt atc agc aat cca ctc aat gtt aac caa aca gcg caa      576 
Pro Gln Ala Arg Ile Ser Asn Pro Leu Asn Val Asn Gln Thr Ala Gln 
            180                 185                 190 

tgg ctg ttg gaa gcc tcg ata tca tcc cac agc gaa cag att tat tat      624 
Trp Leu Leu Glu Ala Ser Ile Ser Ser His Ser Glu Gln Ile Tyr Tyr 
        195                 200                 205 

caa tat cgc gct gaa gat gaa gca ggt tgt gaa acc gac gag cta gca      672 
Gln Tyr Arg Ala Glu Asp Glu Ala Gly Cys Glu Thr Asp Glu Leu Ala 
    210                 215                 220 

gcc cac ccc agc gca acc gtt cag cgc tac ctg caa aca gta cat tac      720 
Ala His Pro Ser Ala Thr Val Gln Arg Tyr Leu Gln Thr Val His Tyr 
225                 230                 235                 240 

ggg aac ctg acc gcc agc gac gtt ttt cct aca cta aac gga gat gac      768 
Gly Asn Leu Thr Ala Ser Asp Val Phe Pro Thr Leu Asn Gly Asp Asp 
                245                 250                 255 

cca ctt aaa tct ggc tgg atg ttc tgt tta gta ttt gac tac ggt gag      816 
Pro Leu Lys Ser Gly Trp Met Phe Cys Leu Val Phe Asp Tyr Gly Glu 
            260                 265                 270 

cgc aaa aac agc tta tct gaa atg ccg ctg ttt aaa gcc aca ggc aat      864 
Arg Lys Asn Ser Leu Ser Glu Met Pro Leu Phe Lys Ala Thr Gly Asn 
        275                 280                 285 

tgg ctt tgc cga aaa gac cgt ttt tcc cgt tat gag tac ggt ttt gaa      912 
Trp Leu Cys Arg Lys Asp Arg Phe Ser Arg Tyr Glu Tyr Gly Phe Glu 
    290                 295                 300 

ttg cgt act cgc cgc tta tgc cgc caa ata ctg atg ttt cac cgt cta      960 
Leu Arg Thr Arg Arg Leu Cys Arg Gln Ile Leu Met Phe His Arg Leu 
305                 310                 315                 320 

caa acc cta tct ggt cag gca aag ggg gat gat gaa cct gcg cta gtg     1008 
Gln Thr Leu Ser Gly Gln Ala Lys Gly Asp Asp Glu Pro Ala Leu Val 
                325                 330                 335 

tcg cgt ctg ata ctg gat tat gac gaa aac gcg atg gtc agt acg ctc     1056 
Ser Arg Leu Ile Leu Asp Tyr Asp Glu Asn Ala Met Val Ser Thr Leu 
            340                 345                 350 

gtt tct gtc cgc cgg gta ggc cat gag gac aac aac acg gtt acc gcg     1104 
Val Ser Val Arg Arg Val Gly His Glu Asp Asn Asn Thr Val Thr Ala 
        355                 360                 365 

ctg cca cca ctg gaa ctg gcc tat cag cct ttt gag cca gaa caa acc     1152 
Leu Pro Pro Leu Glu Leu Ala Tyr Gln Pro Phe Glu Pro Glu Gln Thr 
    370                 375                 380 

gca ctc tgg caa tca atg gat gta ctg gca aat ttc aac acc att cag     1200 
Ala Leu Trp Gln Ser Met Asp Val Leu Ala Asn Phe Asn Thr Ile Gln 
385                 390                 395                 400 

cgc tgg caa ctg ctt gac ctg aaa gga gaa ggc gtg ccc ggc att ctc     1248 
Arg Trp Gln Leu Leu Asp Leu Lys Gly Glu Gly Val Pro Gly Ile Leu 
                405                 410                 415 

tat cag gat aga aat ggc tgg tgg tat cga tct gcc caa cgt cag gcc     1296 
Tyr Gln Asp Arg Asn Gly Trp Trp Tyr Arg Ser Ala Gln Arg Gln Ala 
            420                 425                 430 

ggg gaa gag atg aat gcg gtc acc tgg ggg aaa atg caa ctc ctt ccc     1344 
Gly Glu Glu Met Asn Ala Val Thr Trp Gly Lys Met Gln Leu Leu Pro 
        435                 440                 445 

atc aca cca gct gtg cag gat aac gcc tca ctg atg gat att aac ggt     1392 
Ile Thr Pro Ala Val Gln Asp Asn Ala Ser Leu Met Asp Ile Asn Gly 
    450                 455                 460 

gac ggg caa ctg gac tgg gtg att acc ggg ccg ggg cta agg ggc tat     1440 
Asp Gly Gln Leu Asp Trp Val Ile Thr Gly Pro Gly Leu Arg Gly Tyr 
465                 470                 475                 480 

cac agc caa cac ccg gat ggc agt tgg acg cgt ttt acg cca tta cat     1488 
His Ser Gln His Pro Asp Gly Ser Trp Thr Arg Phe Thr Pro Leu His 
                485                 490                 495 

gcc ctg ccg ata gaa tat tct cat cct cgc gct caa ctt gcc gat tta     1536 
Ala Leu Pro Ile Glu Tyr Ser His Pro Arg Ala Gln Leu Ala Asp Leu 
            500                 505                 510 

atg gga gcc ggg ctg tcc gat tta gtg cta att ggt ccc aaa agt gtg     1584 
Met Gly Ala Gly Leu Ser Asp Leu Val Leu Ile Gly Pro Lys Ser Val 
        515                 520                 525 

cgc tta tat gtc aat aac cgt gat ggt ttt acc gaa ggg cgg gat gtg     1632 
Arg Leu Tyr Val Asn Asn Arg Asp Gly Phe Thr Glu Gly Arg Asp Val 
    530                 535                 540 

gtg caa tcc ggt gat atc acc ctg ccg cta ccg ggc gcc gat gcc cgt     1680 
Val Gln Ser Gly Asp Ile Thr Leu Pro Leu Pro Gly Ala Asp Ala Arg 
545                 550                 555                 560 

aag tta gtg gca ttt agt gac gta ctg ggt tca ggc caa gca cat ctg     1728 
Lys Leu Val Ala Phe Ser Asp Val Leu Gly Ser Gly Gln Ala His Leu 
                565                 570                 575 

gtt gaa gtt agt gca act caa gtc acc tgc tgg ccg aat ctg ggg cat     1776 
Val Glu Val Ser Ala Thr Gln Val Thr Cys Trp Pro Asn Leu Gly His 
            580                 585                 590 

ggc cgt ttt ggt cag cca atc gta ttg ccg gga ttc agc caa tct gcc     1824 
Gly Arg Phe Gly Gln Pro Ile Val Leu Pro Gly Phe Ser Gln Ser Ala 
        595                 600                 605 

gcc agt ttt aat cct gat cga gtt cat ctg gcc gat ttg gat ggg agc     1872 
Ala Ser Phe Asn Pro Asp Arg Val His Leu Ala Asp Leu Asp Gly Ser 
    610                 615                 620 

ggc cct gcc gat ttg att tat gtt cat gct gac cgt ctg gat att ttc     1920 
Gly Pro Ala Asp Leu Ile Tyr Val His Ala Asp Arg Leu Asp Ile Phe 
625                 630                 635                 640 

agc aat gaa agt ggc aac ggt ttt gca aaa cca ttc aca ctc tct ttt     1968 
Ser Asn Glu Ser Gly Asn Gly Phe Ala Lys Pro Phe Thr Leu Ser Phe 
                645                 650                 655 

cct gac ggc ctg cgt ttt gat gat acc tgc cag ttg caa gta gcc gat     2016 
Pro Asp Gly Leu Arg Phe Asp Asp Thr Cys Gln Leu Gln Val Ala Asp 
            660                 665                 670 

gta caa ggg tta ggc gtt gtc agc ctg atc cta agc gta ccg cat atg     2064 
Val Gln Gly Leu Gly Val Val Ser Leu Ile Leu Ser Val Pro His Met 
        675                 680                 685 

gcg cca cat cat tgg cgc tgc gat ctg acc aac gcg aaa ccg tgg tta     2112 
Ala Pro His His Trp Arg Cys Asp Leu Thr Asn Ala Lys Pro Trp Leu 
    690                 695                 700 

ctc agt gaa acg aac aac aat atg ggg gcc aat cac acc ttg cat tac     2160 
Leu Ser Glu Thr Asn Asn Asn Met Gly Ala Asn His Thr Leu His Tyr 
705                 710                 715                 720 

cgt agc tct gtc cag ttc tgg ctg gat gaa aaa gct gcg gca ttg gct     2208 
Arg Ser Ser Val Gln Phe Trp Leu Asp Glu Lys Ala Ala Ala Leu Ala 
                725                 730                 735 

acc gga caa aca ccg gtc tgt tac ctg ccc ttc ccg gtc cat acc ctt     2256 
Thr Gly Gln Thr Pro Val Cys Tyr Leu Pro Phe Pro Val His Thr Leu 
            740                 745                 750 

tgg caa aca gaa acc gag gat gaa atc agc ggc aat aag tta gtg acc     2304 
Trp Gln Thr Glu Thr Glu Asp Glu Ile Ser Gly Asn Lys Leu Val Thr 
        755                 760                 765 

acg tta cgt tat gct cac ggc gct tgg gat gga cgt gaa cgg gaa ttt     2352 
Thr Leu Arg Tyr Ala His Gly Ala Trp Asp Gly Arg Glu Arg Glu Phe 
    770                 775                 780 

cgt ggc ttt ggt tat gtt gag cag aca gac agc cat caa ctc gct caa     2400 
Arg Gly Phe Gly Tyr Val Glu Gln Thr Asp Ser His Gln Leu Ala Gln 
785                 790                 795                 800 

ggc aat gcg ccg gaa cgt aca cca ccg gca ctc acc aaa agc tgg tat     2448 
Gly Asn Ala Pro Glu Arg Thr Pro Pro Ala Leu Thr Lys Ser Trp Tyr 
                805                 810                 815 

gcc acc gga tta cct gcg gta gat aat gcg tta tcc gcc ggg tat tgg     2496 
Ala Thr Gly Leu Pro Ala Val Asp Asn Ala Leu Ser Ala Gly Tyr Trp 
            820                 825                 830 

cgt ggc gat aag caa gct ttc gcc ggt ttt acg cca cgt ttt act ctc     2544 
Arg Gly Asp Lys Gln Ala Phe Ala Gly Phe Thr Pro Arg Phe Thr Leu 
        835                 840                 845 

tgg aaa gag ggc aaa gat gtt cca ctg aca ccg gaa gat gac cat aat     2592 
Trp Lys Glu Gly Lys Asp Val Pro Leu Thr Pro Glu Asp Asp His Asn 
    850                 855                 860 

cta tac tgg tta aac cgg gcg cta aaa ggt cag cca ctg cgt agt gaa     2640 
Leu Tyr Trp Leu Asn Arg Ala Leu Lys Gly Gln Pro Leu Arg Ser Glu 
865                 870                 875                 880 

ctc tac ggg ctg gat ggc agc gca cag caa cag atc ccc tat aca gtg     2688 
Leu Tyr Gly Leu Asp Gly Ser Ala Gln Gln Gln Ile Pro Tyr Thr Val 
                885                 890                 895 

act gaa tcc cgt cca cag gtg cgc caa tta caa gat ggc gcc acc gtt     2736 
Thr Glu Ser Arg Pro Gln Val Arg Gln Leu Gln Asp Gly Ala Thr Val 
            900                 905                 910 

tcc ccg gtg ctc tgg gcc tca gtc gtg gaa agc cgt agt tat cac tac     2784 
Ser Pro Val Leu Trp Ala Ser Val Val Glu Ser Arg Ser Tyr His Tyr 
        915                 920                 925 

gaa cgt att atc agt gat ccc cag tgc aat cag gat atc acg ttg tcc     2832 
Glu Arg Ile Ile Ser Asp Pro Gln Cys Asn Gln Asp Ile Thr Leu Ser 
    930                 935                 940 

agt gac cta ttc ggg caa cca ctg aaa cag gtt tcc gta caa tat ccc     2880 
Ser Asp Leu Phe Gly Gln Pro Leu Lys Gln Val Ser Val Gln Tyr Pro 
945                 950                 955                 960 

cgc cgc aac aaa cca aca acc aat ccg tat ccc gat acc cta ccg gat     2928 
Arg Arg Asn Lys Pro Thr Thr Asn Pro Tyr Pro Asp Thr Leu Pro Asp 
                965                 970                 975 

acg ctg ttt gcc agc agt tat gac gat caa caa cag cta ttg cga tta     2976 
Thr Leu Phe Ala Ser Ser Tyr Asp Asp Gln Gln Gln Leu Leu Arg Leu 
            980                 985                 990 

acc tgc cga caa tcc agt tgg cac  cat ctt att ggt aat  gag cta aga   3024 
Thr Cys Arg Gln Ser Ser Trp His  His Leu Ile Gly Asn  Glu Leu Arg 
        995                 1000                 1005 

gtg ttg  gga tta ccg gat ggc  aca cgc agt gat gcc  ttt act tac      3069 
Val Leu  Gly Leu Pro Asp Gly  Thr Arg Ser Asp Ala  Phe Thr Tyr 
    1010                 1015                 1020 

gat gcc  aaa cag gta cct gtc  gat ggc tta aat ctg  gaa acc ctg      3114 
Asp Ala  Lys Gln Val Pro Val  Asp Gly Leu Asn Leu  Glu Thr Leu 
    1025                 1030                 1035 

tgt gct  gaa aat agc ctg att  gcc gat gat aaa cct  cgc gaa tac      3159 
Cys Ala  Glu Asn Ser Leu Ile  Ala Asp Asp Lys Pro  Arg Glu Tyr 
    1040                 1045                 1050 

ctc aat  cag caa cga acg ttc  tat acc gac ggg aaa  aac caa aca      3204 
Leu Asn  Gln Gln Arg Thr Phe  Tyr Thr Asp Gly Lys  Asn Gln Thr 
    1055                 1060                 1065 

ccg ctg  aaa aca ccg aca cga  caa gcg tta atc gcc  ttt acc gaa      3249 
Pro Leu  Lys Thr Pro Thr Arg  Gln Ala Leu Ile Ala  Phe Thr Glu 
    1070                 1075                 1080 

acg gcg  gta tta acg gaa tct  ctg tta tcc gcg ttt  gat ggc ggt      3294 
Thr Ala  Val Leu Thr Glu Ser  Leu Leu Ser Ala Phe  Asp Gly Gly 
    1085                 1090                 1095 

att acg  cca gac gaa tta ccg  gga ata ctg aca cag  gcc gga tac      3339 
Ile Thr  Pro Asp Glu Leu Pro  Gly Ile Leu Thr Gln  Ala Gly Tyr 
    1100                 1105                 1110 

caa caa  gag cct tat ctg ttt  cca cgc acc ggc gaa  aac aaa gtt      3384 
Gln Gln  Glu Pro Tyr Leu Phe  Pro Arg Thr Gly Glu  Asn Lys Val 
    1115                 1120                 1125 

tgg gta  gcg cgt caa ggc tat  acc gat tac ggg acg  gaa gca caa      3429 
Trp Val  Ala Arg Gln Gly Tyr  Thr Asp Tyr Gly Thr  Glu Ala Gln 
    1130                 1135                 1140 

ttt tgg  cgt cct gtc gca caa  cgt aac agc ctg tta  acc ggg aaa      3474 
Phe Trp  Arg Pro Val Ala Gln  Arg Asn Ser Leu Leu  Thr Gly Lys 
    1145                 1150                 1155 

atg acg  tta aaa tgg gat act  cac tat tgt gtc atc  acc caa acc      3519 
Met Thr  Leu Lys Trp Asp Thr  His Tyr Cys Val Ile  Thr Gln Thr 
    1160                 1165                 1170 

caa gat  gct gcc ggc ctc acc  gtc tca gcc aat tat  gac tgg cgt      3564 
Gln Asp  Ala Ala Gly Leu Thr  Val Ser Ala Asn Tyr  Asp Trp Arg 
    1175                 1180                 1185 

ttt ctc  aca cca acg caa ctg  act gac atc aac gat  aat gtg cat      3609 
Phe Leu  Thr Pro Thr Gln Leu  Thr Asp Ile Asn Asp  Asn Val His 
    1190                 1195                 1200 

ctc atc  acc ttg gat gct ctg  gga cgc cct gtc acg  caa cgt ttc      3654 
Leu Ile  Thr Leu Asp Ala Leu  Gly Arg Pro Val Thr  Gln Arg Phe 
    1205                 1210                 1215 

tgg ggg  atc gaa agc ggt gtg  gca aca ggt tac tct  tca tca gaa      3699 
Trp Gly  Ile Glu Ser Gly Val  Ala Thr Gly Tyr Ser  Ser Ser Glu 
    1220                 1225                 1230 

gaa aaa  cca ttc tct cca cca  aac gat atc gat acc  gct att aat      3744 
Glu Lys  Pro Phe Ser Pro Pro  Asn Asp Ile Asp Thr  Ala Ile Asn 
    1235                 1240                 1245 

cta acc  gga cca ctc cct gtc  gca cag tgt ctg gtc  tat gca ccg      3789 
Leu Thr  Gly Pro Leu Pro Val  Ala Gln Cys Leu Val  Tyr Ala Pro 
    1250                 1255                 1260 

gac agt  tgg atg cca cta ttc  agt caa gaa acc ttc  aac aca tta      3834 
Asp Ser  Trp Met Pro Leu Phe  Ser Gln Glu Thr Phe  Asn Thr Leu 
    1265                 1270                 1275 

acg cag  gaa gag cag gag acg  ctg cgt gat tca cgt  att atc acg      3879 
Thr Gln  Glu Glu Gln Glu Thr  Leu Arg Asp Ser Arg  Ile Ile Thr 
    1280                 1285                 1290 

gaa gat  tgg cgt att tgc gca  ctg act cgc cgc cgt  tgg cta caa      3924 
Glu Asp  Trp Arg Ile Cys Ala  Leu Thr Arg Arg Arg  Trp Leu Gln 
    1295                 1300                 1305 

agt caa  aag atc agt aca cca  tta gtt aaa ctg tta  acc aac agc      3969 
Ser Gln  Lys Ile Ser Thr Pro  Leu Val Lys Leu Leu  Thr Asn Ser 
    1310                 1315                 1320 

att ggt  tta cct ccc cat aac  ctt acg ctg acc aca  gac cgt tat      4014 
Ile Gly  Leu Pro Pro His Asn  Leu Thr Leu Thr Thr  Asp Arg Tyr 
    1325                 1330                 1335 

gac cgc  gac tct gag cag caa  att cgc caa caa gtc  gca ttt agt      4059 
Asp Arg  Asp Ser Glu Gln Gln  Ile Arg Gln Gln Val  Ala Phe Ser 
    1340                 1345                 1350 

gat ggt  ttt ggc cgt ctg cta  caa gcg tct gta cga  cat gag gca      4104 
Asp Gly  Phe Gly Arg Leu Leu  Gln Ala Ser Val Arg  His Glu Ala 
    1355                 1360                 1365 

ggc gaa  gcc tgg caa cgt aac  caa gac ggt tct ctg  gtg aca aaa      4149 
Gly Glu  Ala Trp Gln Arg Asn  Gln Asp Gly Ser Leu  Val Thr Lys 
    1370                 1375                 1380 

gtg gag  aat acc aaa acg cgt  tgg gcg gtc acg gga  cgc acc gaa      4194 
Val Glu  Asn Thr Lys Thr Arg  Trp Ala Val Thr Gly  Arg Thr Glu 
    1385                 1390                 1395 

tat gat  aat aaa ggg caa acg  ata cgc act tat cag  ccc tat ttc      4239 
Tyr Asp  Asn Lys Gly Gln Thr  Ile Arg Thr Tyr Gln  Pro Tyr Phe 
    1400                 1405                 1410 

ctc aac  gac tgg cga tat gtc  agt gat gac agc gcc  aga aaa gaa      4284 
Leu Asn  Asp Trp Arg Tyr Val  Ser Asp Asp Ser Ala  Arg Lys Glu 
    1415                 1420                 1425 

gcc tat  gcg gat act cat att  tat gat cca att ggg  cga gaa atc      4329 
Ala Tyr  Ala Asp Thr His Ile  Tyr Asp Pro Ile Gly  Arg Glu Ile 
    1430                 1435                 1440 

cgg gtt  att act gca aaa ggc  tgg ctg cgc caa agc  caa tat ttc      4374 
Arg Val  Ile Thr Ala Lys Gly  Trp Leu Arg Gln Ser  Gln Tyr Phe 
    1445                 1450                 1455 

ccg tgg  ttt acc gtg agt gag  gat gag aat gat acg  gcc gct gat      4419 
Pro Trp  Phe Thr Val Ser Glu  Asp Glu Asn Asp Thr  Ala Ala Asp 
    1460                 1465                 1470 

gcg ctg  gtg taa                                                    4431 
Ala Leu  Val 
    1475 

 
           
             4  
             1476  
             PRT  
             Photorhabdus luminescens  
           
            4 

Met Gln Asn Ser Gln Thr Phe Ser Val Thr Glu Leu Ser Leu Pro Lys 
1               5                   10                  15 

Gly Gly Gly Ala Ile Thr Gly Met Gly Glu Ala Leu Thr Pro Ala Gly 
            20                  25                  30 

Pro Asp Gly Met Ala Ala Leu Ser Leu Pro Leu Pro Ile Ser Ala Gly 
        35                  40                  45 

Arg Gly Tyr Ala Pro Ser Leu Thr Leu Asn Tyr Asn Ser Gly Thr Gly 
    50                  55                  60 

Asn Ser Pro Phe Gly Leu Gly Trp Asp Cys Gly Val Met Ala Ile Arg 
65                  70                  75                  80 

Arg Arg Thr Ser Thr Gly Val Pro Asn Tyr Asp Glu Thr Asp Thr Phe 
                85                  90                  95 

Leu Gly Pro Glu Gly Glu Val Leu Val Val Ala Leu Asn Glu Ala Gly 
            100                 105                 110 

Gln Ala Asp Ile Arg Ser Glu Ser Ser Leu Gln Gly Ile Asn Leu Gly 
        115                 120                 125 

Ala Thr Phe Thr Val Thr Cys Tyr Arg Ser Arg Leu Glu Ser His Phe 
    130                 135                 140 

Asn Arg Leu Glu Tyr Trp Gln Pro Gln Thr Thr Gly Ala Thr Asp Phe 
145                 150                 155                 160 

Trp Leu Ile Tyr Ser Pro Asp Gly Gln Val His Leu Leu Gly Lys Asn 
                165                 170                 175 

Pro Gln Ala Arg Ile Ser Asn Pro Leu Asn Val Asn Gln Thr Ala Gln 
            180                 185                 190 

Trp Leu Leu Glu Ala Ser Ile Ser Ser His Ser Glu Gln Ile Tyr Tyr 
        195                 200                 205 

Gln Tyr Arg Ala Glu Asp Glu Ala Gly Cys Glu Thr Asp Glu Leu Ala 
    210                 215                 220 

Ala His Pro Ser Ala Thr Val Gln Arg Tyr Leu Gln Thr Val His Tyr 
225                 230                 235                 240 

Gly Asn Leu Thr Ala Ser Asp Val Phe Pro Thr Leu Asn Gly Asp Asp 
                245                 250                 255 

Pro Leu Lys Ser Gly Trp Met Phe Cys Leu Val Phe Asp Tyr Gly Glu 
            260                 265                 270 

Arg Lys Asn Ser Leu Ser Glu Met Pro Leu Phe Lys Ala Thr Gly Asn 
        275                 280                 285 

Trp Leu Cys Arg Lys Asp Arg Phe Ser Arg Tyr Glu Tyr Gly Phe Glu 
    290                 295                 300 

Leu Arg Thr Arg Arg Leu Cys Arg Gln Ile Leu Met Phe His Arg Leu 
305                 310                 315                 320 

Gln Thr Leu Ser Gly Gln Ala Lys Gly Asp Asp Glu Pro Ala Leu Val 
                325                 330                 335 

Ser Arg Leu Ile Leu Asp Tyr Asp Glu Asn Ala Met Val Ser Thr Leu 
            340                 345                 350 

Val Ser Val Arg Arg Val Gly His Glu Asp Asn Asn Thr Val Thr Ala 
        355                 360                 365 

Leu Pro Pro Leu Glu Leu Ala Tyr Gln Pro Phe Glu Pro Glu Gln Thr 
    370                 375                 380 

Ala Leu Trp Gln Ser Met Asp Val Leu Ala Asn Phe Asn Thr Ile Gln 
385                 390                 395                 400 

Arg Trp Gln Leu Leu Asp Leu Lys Gly Glu Gly Val Pro Gly Ile Leu 
                405                 410                 415 

Tyr Gln Asp Arg Asn Gly Trp Trp Tyr Arg Ser Ala Gln Arg Gln Ala 
            420                 425                 430 

Gly Glu Glu Met Asn Ala Val Thr Trp Gly Lys Met Gln Leu Leu Pro 
        435                 440                 445 

Ile Thr Pro Ala Val Gln Asp Asn Ala Ser Leu Met Asp Ile Asn Gly 
    450                 455                 460 

Asp Gly Gln Leu Asp Trp Val Ile Thr Gly Pro Gly Leu Arg Gly Tyr 
465                 470                 475                 480 

His Ser Gln His Pro Asp Gly Ser Trp Thr Arg Phe Thr Pro Leu His 
                485                 490                 495 

Ala Leu Pro Ile Glu Tyr Ser His Pro Arg Ala Gln Leu Ala Asp Leu 
            500                 505                 510 

Met Gly Ala Gly Leu Ser Asp Leu Val Leu Ile Gly Pro Lys Ser Val 
        515                 520                 525 

Arg Leu Tyr Val Asn Asn Arg Asp Gly Phe Thr Glu Gly Arg Asp Val 
    530                 535                 540 

Val Gln Ser Gly Asp Ile Thr Leu Pro Leu Pro Gly Ala Asp Ala Arg 
545                 550                 555                 560 

Lys Leu Val Ala Phe Ser Asp Val Leu Gly Ser Gly Gln Ala His Leu 
                565                 570                 575 

Val Glu Val Ser Ala Thr Gln Val Thr Cys Trp Pro Asn Leu Gly His 
            580                 585                 590 

Gly Arg Phe Gly Gln Pro Ile Val Leu Pro Gly Phe Ser Gln Ser Ala 
        595                 600                 605 

Ala Ser Phe Asn Pro Asp Arg Val His Leu Ala Asp Leu Asp Gly Ser 
    610                 615                 620 

Gly Pro Ala Asp Leu Ile Tyr Val His Ala Asp Arg Leu Asp Ile Phe 
625                 630                 635                 640 

Ser Asn Glu Ser Gly Asn Gly Phe Ala Lys Pro Phe Thr Leu Ser Phe 
                645                 650                 655 

Pro Asp Gly Leu Arg Phe Asp Asp Thr Cys Gln Leu Gln Val Ala Asp 
            660                 665                 670 

Val Gln Gly Leu Gly Val Val Ser Leu Ile Leu Ser Val Pro His Met 
        675                 680                 685 

Ala Pro His His Trp Arg Cys Asp Leu Thr Asn Ala Lys Pro Trp Leu 
    690                 695                 700 

Leu Ser Glu Thr Asn Asn Asn Met Gly Ala Asn His Thr Leu His Tyr 
705                 710                 715                 720 

Arg Ser Ser Val Gln Phe Trp Leu Asp Glu Lys Ala Ala Ala Leu Ala 
                725                 730                 735 

Thr Gly Gln Thr Pro Val Cys Tyr Leu Pro Phe Pro Val His Thr Leu 
            740                 745                 750 

Trp Gln Thr Glu Thr Glu Asp Glu Ile Ser Gly Asn Lys Leu Val Thr 
        755                 760                 765 

Thr Leu Arg Tyr Ala His Gly Ala Trp Asp Gly Arg Glu Arg Glu Phe 
    770                 775                 780 

Arg Gly Phe Gly Tyr Val Glu Gln Thr Asp Ser His Gln Leu Ala Gln 
785                 790                 795                 800 

Gly Asn Ala Pro Glu Arg Thr Pro Pro Ala Leu Thr Lys Ser Trp Tyr 
                805                 810                 815 

Ala Thr Gly Leu Pro Ala Val Asp Asn Ala Leu Ser Ala Gly Tyr Trp 
            820                 825                 830 

Arg Gly Asp Lys Gln Ala Phe Ala Gly Phe Thr Pro Arg Phe Thr Leu 
        835                 840                 845 

Trp Lys Glu Gly Lys Asp Val Pro Leu Thr Pro Glu Asp Asp His Asn 
    850                 855                 860 

Leu Tyr Trp Leu Asn Arg Ala Leu Lys Gly Gln Pro Leu Arg Ser Glu 
865                 870                 875                 880 

Leu Tyr Gly Leu Asp Gly Ser Ala Gln Gln Gln Ile Pro Tyr Thr Val 
                885                 890                 895 

Thr Glu Ser Arg Pro Gln Val Arg Gln Leu Gln Asp Gly Ala Thr Val 
            900                 905                 910 

Ser Pro Val Leu Trp Ala Ser Val Val Glu Ser Arg Ser Tyr His Tyr 
        915                 920                 925 

Glu Arg Ile Ile Ser Asp Pro Gln Cys Asn Gln Asp Ile Thr Leu Ser 
    930                 935                 940 

Ser Asp Leu Phe Gly Gln Pro Leu Lys Gln Val Ser Val Gln Tyr Pro 
945                 950                 955                 960 

Arg Arg Asn Lys Pro Thr Thr Asn Pro Tyr Pro Asp Thr Leu Pro Asp 
                965                 970                 975 

Thr Leu Phe Ala Ser Ser Tyr Asp Asp Gln Gln Gln Leu Leu Arg Leu 
            980                 985                 990 

Thr Cys Arg Gln Ser Ser Trp His  His Leu Ile Gly Asn  Glu Leu Arg 
        995                 1000                 1005 

Val Leu  Gly Leu Pro Asp Gly  Thr Arg Ser Asp Ala  Phe Thr Tyr 
    1010                 1015                 1020 

Asp Ala  Lys Gln Val Pro Val  Asp Gly Leu Asn Leu  Glu Thr Leu 
    1025                 1030                 1035 

Cys Ala  Glu Asn Ser Leu Ile  Ala Asp Asp Lys Pro  Arg Glu Tyr 
    1040                 1045                 1050 

Leu Asn  Gln Gln Arg Thr Phe  Tyr Thr Asp Gly Lys  Asn Gln Thr 
    1055                 1060                 1065 

Pro Leu  Lys Thr Pro Thr Arg  Gln Ala Leu Ile Ala  Phe Thr Glu 
    1070                 1075                 1080 

Thr Ala  Val Leu Thr Glu Ser  Leu Leu Ser Ala Phe  Asp Gly Gly 
    1085                 1090                 1095 

Ile Thr  Pro Asp Glu Leu Pro  Gly Ile Leu Thr Gln  Ala Gly Tyr 
    1100                 1105                 1110 

Gln Gln  Glu Pro Tyr Leu Phe  Pro Arg Thr Gly Glu  Asn Lys Val 
    1115                 1120                 1125 

Trp Val  Ala Arg Gln Gly Tyr  Thr Asp Tyr Gly Thr  Glu Ala Gln 
    1130                 1135                 1140 

Phe Trp  Arg Pro Val Ala Gln  Arg Asn Ser Leu Leu  Thr Gly Lys 
    1145                 1150                 1155 

Met Thr  Leu Lys Trp Asp Thr  His Tyr Cys Val Ile  Thr Gln Thr 
    1160                 1165                 1170 

Gln Asp  Ala Ala Gly Leu Thr  Val Ser Ala Asn Tyr  Asp Trp Arg 
    1175                 1180                 1185 

Phe Leu  Thr Pro Thr Gln Leu  Thr Asp Ile Asn Asp  Asn Val His 
    1190                 1195                 1200 

Leu Ile  Thr Leu Asp Ala Leu  Gly Arg Pro Val Thr  Gln Arg Phe 
    1205                 1210                 1215 

Trp Gly  Ile Glu Ser Gly Val  Ala Thr Gly Tyr Ser  Ser Ser Glu 
    1220                 1225                 1230 

Glu Lys  Pro Phe Ser Pro Pro  Asn Asp Ile Asp Thr  Ala Ile Asn 
    1235                 1240                 1245 

Leu Thr  Gly Pro Leu Pro Val  Ala Gln Cys Leu Val  Tyr Ala Pro 
    1250                 1255                 1260 

Asp Ser  Trp Met Pro Leu Phe  Ser Gln Glu Thr Phe  Asn Thr Leu 
    1265                 1270                 1275 

Thr Gln  Glu Glu Gln Glu Thr  Leu Arg Asp Ser Arg  Ile Ile Thr 
    1280                 1285                 1290 

Glu Asp  Trp Arg Ile Cys Ala  Leu Thr Arg Arg Arg  Trp Leu Gln 
    1295                 1300                 1305 

Ser Gln  Lys Ile Ser Thr Pro  Leu Val Lys Leu Leu  Thr Asn Ser 
    1310                 1315                 1320 

Ile Gly  Leu Pro Pro His Asn  Leu Thr Leu Thr Thr  Asp Arg Tyr 
    1325                 1330                 1335 

Asp Arg  Asp Ser Glu Gln Gln  Ile Arg Gln Gln Val  Ala Phe Ser 
    1340                 1345                 1350 

Asp Gly  Phe Gly Arg Leu Leu  Gln Ala Ser Val Arg  His Glu Ala 
    1355                 1360                 1365 

Gly Glu  Ala Trp Gln Arg Asn  Gln Asp Gly Ser Leu  Val Thr Lys 
    1370                 1375                 1380 

Val Glu  Asn Thr Lys Thr Arg  Trp Ala Val Thr Gly  Arg Thr Glu 
    1385                 1390                 1395 

Tyr Asp  Asn Lys Gly Gln Thr  Ile Arg Thr Tyr Gln  Pro Tyr Phe 
    1400                 1405                 1410 

Leu Asn  Asp Trp Arg Tyr Val  Ser Asp Asp Ser Ala  Arg Lys Glu 
    1415                 1420                 1425 

Ala Tyr  Ala Asp Thr His Ile  Tyr Asp Pro Ile Gly  Arg Glu Ile 
    1430                 1435                 1440 

Arg Val  Ile Thr Ala Lys Gly  Trp Leu Arg Gln Ser  Gln Tyr Phe 
    1445                 1450                 1455 

Pro Trp  Phe Thr Val Ser Glu  Asp Glu Asn Asp Thr  Ala Ala Asp 
    1460                 1465                 1470 

Ala Leu  Val 
    1475 

 
           
             5  
             2745  
             DNA  
             Photorhabdus luminescens  
             
               CDS  
               (1)..(2745)  
             
           
            5 

atg agc agt tac aat tct gca att gac caa aag acc ccc tcg att aag       48 
Met Ser Ser Tyr Asn Ser Ala Ile Asp Gln Lys Thr Pro Ser Ile Lys 
1               5                   10                  15 

gta tta gat aac agg aaa tta aat gta cgt act tta gaa tat cta cgc       96 
Val Leu Asp Asn Arg Lys Leu Asn Val Arg Thr Leu Glu Tyr Leu Arg 
            20                  25                  30 

act caa gct gac gaa aac agt gat gaa tta att acg ttc tat gag ttc      144 
Thr Gln Ala Asp Glu Asn Ser Asp Glu Leu Ile Thr Phe Tyr Glu Phe 
        35                  40                  45 

aat att ccg gga ttt cag gta aaa agc acc gat cct cgt aaa aat aaa      192 
Asn Ile Pro Gly Phe Gln Val Lys Ser Thr Asp Pro Arg Lys Asn Lys 
    50                  55                  60 

aac cag agc ggc cca aat ttc att cgt gtc ttt aat ctt gcc ggt caa      240 
Asn Gln Ser Gly Pro Asn Phe Ile Arg Val Phe Asn Leu Ala Gly Gln 
65                  70                  75                  80 

gtt tta cgt gaa gaa agt gtt gat gcc ggt cgg act att acc ctc aat      288 
Val Leu Arg Glu Glu Ser Val Asp Ala Gly Arg Thr Ile Thr Leu Asn 
                85                  90                  95 

gat att gaa agt cgc ccg gtg ttg atc atc aat gca acc ggt gtc cgc      336 
Asp Ile Glu Ser Arg Pro Val Leu Ile Ile Asn Ala Thr Gly Val Arg 
            100                 105                 110 

caa aac cat cgt tat gaa gat aac acc ctt ccc ggt cgt ctg ctc gct      384 
Gln Asn His Arg Tyr Glu Asp Asn Thr Leu Pro Gly Arg Leu Leu Ala 
        115                 120                 125 

atc acc gaa caa gta cag gca gga gag aaa acg acc gaa cgt ctt atc      432 
Ile Thr Glu Gln Val Gln Ala Gly Glu Lys Thr Thr Glu Arg Leu Ile 
    130                 135                 140 

tgg gcc ggc aat acg ccg caa gaa aaa gat tac aac ctc gcc ggt cag      480 
Trp Ala Gly Asn Thr Pro Gln Glu Lys Asp Tyr Asn Leu Ala Gly Gln 
145                 150                 155                 160 

tgt gtc cgc cat tac gat acc gcg gga ctt act caa ctc aat agc ctt      528 
Cys Val Arg His Tyr Asp Thr Ala Gly Leu Thr Gln Leu Asn Ser Leu 
                165                 170                 175 

tct ctg gct ggc gtc gtg cta tca caa tct caa caa ctg ctt acc gat      576 
Ser Leu Ala Gly Val Val Leu Ser Gln Ser Gln Gln Leu Leu Thr Asp 
            180                 185                 190 

aac cag gat gcc gac tgg aca ggt gaa gac cag agc ctc tgg caa caa      624 
Asn Gln Asp Ala Asp Trp Thr Gly Glu Asp Gln Ser Leu Trp Gln Gln 
        195                 200                 205 

aaa ctg agt agt gat gtc tat atc acc caa agt aac act gat gcc acc      672 
Lys Leu Ser Ser Asp Val Tyr Ile Thr Gln Ser Asn Thr Asp Ala Thr 
    210                 215                 220 

ggg gct tta ctg acc cag acc gat gcc aaa ggc aac att cag cgg ctg      720 
Gly Ala Leu Leu Thr Gln Thr Asp Ala Lys Gly Asn Ile Gln Arg Leu 
225                 230                 235                 240 

gcc tat gat gtg gcc ggg cag cta aaa ggg agt tgg tta aca ctc aaa      768 
Ala Tyr Asp Val Ala Gly Gln Leu Lys Gly Ser Trp Leu Thr Leu Lys 
                245                 250                 255 

ggt cag gcg gaa cag gtg att atc aaa tcg cta acc tac tcc gcc gcc      816 
Gly Gln Ala Glu Gln Val Ile Ile Lys Ser Leu Thr Tyr Ser Ala Ala 
            260                 265                 270 

ggg caa aaa tta cgt gaa gag cac ggt aac ggg att gtc act gaa tac      864 
Gly Gln Lys Leu Arg Glu Glu His Gly Asn Gly Ile Val Thr Glu Tyr 
        275                 280                 285 

agc tac gaa ccg gaa acc caa cgg ctt atc ggc att acc act cgc cgt      912 
Ser Tyr Glu Pro Glu Thr Gln Arg Leu Ile Gly Ile Thr Thr Arg Arg 
    290                 295                 300 

cca tca gac gcc aag gtg ttg caa gac cta cgc tat caa tat gac cca      960 
Pro Ser Asp Ala Lys Val Leu Gln Asp Leu Arg Tyr Gln Tyr Asp Pro 
305                 310                 315                 320 

gta ggc aat gtc att aat atc cgt aat gat gcg gaa gcc act cgc ttt     1008 
Val Gly Asn Val Ile Asn Ile Arg Asn Asp Ala Glu Ala Thr Arg Phe 
                325                 330                 335 

tgg cgc aat cag aaa gta gcc ccg gag aat agc tat acc tac gat tcc     1056 
Trp Arg Asn Gln Lys Val Ala Pro Glu Asn Ser Tyr Thr Tyr Asp Ser 
            340                 345                 350 

ctg tat cag ctt atc agc gcc acc ggg cgc gaa atg gcc aat atc ggt     1104 
Leu Tyr Gln Leu Ile Ser Ala Thr Gly Arg Glu Met Ala Asn Ile Gly 
        355                 360                 365 

cag caa aac aac caa ctt ccc tcc cct gcg cta cct tct gac aac aat     1152 
Gln Gln Asn Asn Gln Leu Pro Ser Pro Ala Leu Pro Ser Asp Asn Asn 
    370                 375                 380 

acc tac act aac tat act cgc agc tac agc tat gat cac agt ggt aat     1200 
Thr Tyr Thr Asn Tyr Thr Arg Ser Tyr Ser Tyr Asp His Ser Gly Asn 
385                 390                 395                 400 

ctg acg caa att cgg cac agc tcg cca gct acc cag aac aac tac acc     1248 
Leu Thr Gln Ile Arg His Ser Ser Pro Ala Thr Gln Asn Asn Tyr Thr 
                405                 410                 415 

gtg gct atc acc ctc tca aac cgc agc aat cgg ggt gtt ctc agt acg     1296 
Val Ala Ile Thr Leu Ser Asn Arg Ser Asn Arg Gly Val Leu Ser Thr 
            420                 425                 430 

cta acc acc gat cca aat caa gtg gat acg ttg ttt gat gcc ggt ggt     1344 
Leu Thr Thr Asp Pro Asn Gln Val Asp Thr Leu Phe Asp Ala Gly Gly 
        435                 440                 445 

cac caa acc agt tta tta ccc gga cag aca ctt atc tgg aca cca cga     1392 
His Gln Thr Ser Leu Leu Pro Gly Gln Thr Leu Ile Trp Thr Pro Arg 
    450                 455                 460 

gga gag tta aag cag gtt aat aat ggc ccg gga aat gag tgg tac cgc     1440 
Gly Glu Leu Lys Gln Val Asn Asn Gly Pro Gly Asn Glu Trp Tyr Arg 
465                 470                 475                 480 

tac gac agc aac ggc atg aga caa ctg aaa gtg agt gaa cag cca acc     1488 
Tyr Asp Ser Asn Gly Met Arg Gln Leu Lys Val Ser Glu Gln Pro Thr 
                485                 490                 495 

cag aat act acg cag caa caa cgg gta atc tat ttg ccg gga ctg gag     1536 
Gln Asn Thr Thr Gln Gln Gln Arg Val Ile Tyr Leu Pro Gly Leu Glu 
            500                 505                 510 

cta cgc aca acc cag agc aac gcc aca aca acg gaa gag tta cac gtt     1584 
Leu Arg Thr Thr Gln Ser Asn Ala Thr Thr Thr Glu Glu Leu His Val 
        515                 520                 525 

atc aca ctc ggt gaa gcc ggt cgc gca cag gta cgg gtg ttg cac tgg     1632 
Ile Thr Leu Gly Glu Ala Gly Arg Ala Gln Val Arg Val Leu His Trp 
    530                 535                 540 

gag agc ggt aag cca gaa gat gtc aac aat aat caa cta cgt tac agc     1680 
Glu Ser Gly Lys Pro Glu Asp Val Asn Asn Asn Gln Leu Arg Tyr Ser 
545                 550                 555                 560 

tac gat aat ctg atc ggc tcc agc cag ctt gaa ctg gac aac caa gga     1728 
Tyr Asp Asn Leu Ile Gly Ser Ser Gln Leu Glu Leu Asp Asn Gln Gly 
                565                 570                 575 

caa att atc agc gag gaa gag tat tat cca ttt ggc ggg aca gcg ctg     1776 
Gln Ile Ile Ser Glu Glu Glu Tyr Tyr Pro Phe Gly Gly Thr Ala Leu 
            580                 585                 590 

tgg gca gca aac agc caa aca gaa gcc agc tat aaa acg att cgc tat     1824 
Trp Ala Ala Asn Ser Gln Thr Glu Ala Ser Tyr Lys Thr Ile Arg Tyr 
        595                 600                 605 

tcc ggc aaa gaa cga gat gcc acc ggg ttg tat tat tac ggt tat cgt     1872 
Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly Tyr Arg 
    610                 615                 620 

tat tac caa ccg tgg gcg ggc aga tgg tta agc gcg gac ccg gca gga     1920 
Tyr Tyr Gln Pro Trp Ala Gly Arg Trp Leu Ser Ala Asp Pro Ala Gly 
625                 630                 635                 640 

acc att gat ggg ctg aat cta tac cga atg gta aga aat aat cct gtg     1968 
Thr Ile Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn Pro Val 
                645                 650                 655 

agt tta caa gat gaa aat gga tta gcg cca gaa aaa ggg aaa tat acc     2016 
Ser Leu Gln Asp Glu Asn Gly Leu Ala Pro Glu Lys Gly Lys Tyr Thr 
            660                 665                 670 

aaa gag gta aat ttc ttt gat gaa tta aaa ttc aaa ttg gca gcc aaa     2064 
Lys Glu Val Asn Phe Phe Asp Glu Leu Lys Phe Lys Leu Ala Ala Lys 
        675                 680                 685 

agt tca cat gtt gtc aaa tgg aac gag aaa gag agc agt tat aca aaa     2112 
Ser Ser His Val Val Lys Trp Asn Glu Lys Glu Ser Ser Tyr Thr Lys 
    690                 695                 700 

aat aaa tca ttg aaa gtg gtt cgt gtc ggt gat tcc gat ccg tcg ggt     2160 
Asn Lys Ser Leu Lys Val Val Arg Val Gly Asp Ser Asp Pro Ser Gly 
705                 710                 715                 720 

tat ttg cta agc cac gaa gag tta cta aaa ggt ata gaa aaa agt caa     2208 
Tyr Leu Leu Ser His Glu Glu Leu Leu Lys Gly Ile Glu Lys Ser Gln 
                725                 730                 735 

atc ata tat agc cga ctt gaa gaa aac agc tcc ctt tca gaa aaa tca     2256 
Ile Ile Tyr Ser Arg Leu Glu Glu Asn Ser Ser Leu Ser Glu Lys Ser 
            740                 745                 750 

aaa acg aat ctt tct tta gga tct gaa ata tcc ggt tat atg gca aga     2304 
Lys Thr Asn Leu Ser Leu Gly Ser Glu Ile Ser Gly Tyr Met Ala Arg 
        755                 760                 765 

acc ata caa gat acg ata tca gaa tat gcc gaa gag cat aaa tat aga     2352 
Thr Ile Gln Asp Thr Ile Ser Glu Tyr Ala Glu Glu His Lys Tyr Arg 
    770                 775                 780 

agt aat cac cct gat ttt tat tca gaa acc gat ttc ttt gcg tta atg     2400 
Ser Asn His Pro Asp Phe Tyr Ser Glu Thr Asp Phe Phe Ala Leu Met 
785                 790                 795                 800 

gat aaa agt gaa aaa aat gat tat tcc ggt gaa aga aaa att tat gcg     2448 
Asp Lys Ser Glu Lys Asn Asp Tyr Ser Gly Glu Arg Lys Ile Tyr Ala 
                805                 810                 815 

gca atg gag gtt aag gtt tat cat gat tta aaa aat aaa caa tca gaa     2496 
Ala Met Glu Val Lys Val Tyr His Asp Leu Lys Asn Lys Gln Ser Glu 
            820                 825                 830 

tta cat gtc aac tat gca ttg gcc cat ccc tat acg caa ttg agt aat     2544 
Leu His Val Asn Tyr Ala Leu Ala His Pro Tyr Thr Gln Leu Ser Asn 
        835                 840                 845 

gaa gaa aga gcg ctg ttg caa gaa aca gaa ccc gct att gca ata gat     2592 
Glu Glu Arg Ala Leu Leu Gln Glu Thr Glu Pro Ala Ile Ala Ile Asp 
    850                 855                 860 

aga gaa tat aat ttc aaa ggt gtt ggc aaa ttc ctg aca atg aaa gca     2640 
Arg Glu Tyr Asn Phe Lys Gly Val Gly Lys Phe Leu Thr Met Lys Ala 
865                 870                 875                 880 

att aaa aaa tca ttg aaa gga cat aaa att aat agg ata tca aca gag     2688 
Ile Lys Lys Ser Leu Lys Gly His Lys Ile Asn Arg Ile Ser Thr Glu 
                885                 890                 895 

gct att aat att cgc tct gcg gct atc gct gag aat tta gga atg cgg     2736 
Ala Ile Asn Ile Arg Ser Ala Ala Ile Ala Glu Asn Leu Gly Met Arg 
            900                 905                 910 

aga act tca                                                         2745 
Arg Thr Ser 
        915 

 
           
             6  
             915  
             PRT  
             Photorhabdus luminescens  
           
            6 

Met Ser Ser Tyr Asn Ser Ala Ile Asp Gln Lys Thr Pro Ser Ile Lys 
1               5                   10                  15 

Val Leu Asp Asn Arg Lys Leu Asn Val Arg Thr Leu Glu Tyr Leu Arg 
            20                  25                  30 

Thr Gln Ala Asp Glu Asn Ser Asp Glu Leu Ile Thr Phe Tyr Glu Phe 
        35                  40                  45 

Asn Ile Pro Gly Phe Gln Val Lys Ser Thr Asp Pro Arg Lys Asn Lys 
    50                  55                  60 

Asn Gln Ser Gly Pro Asn Phe Ile Arg Val Phe Asn Leu Ala Gly Gln 
65                  70                  75                  80 

Val Leu Arg Glu Glu Ser Val Asp Ala Gly Arg Thr Ile Thr Leu Asn 
                85                  90                  95 

Asp Ile Glu Ser Arg Pro Val Leu Ile Ile Asn Ala Thr Gly Val Arg 
            100                 105                 110 

Gln Asn His Arg Tyr Glu Asp Asn Thr Leu Pro Gly Arg Leu Leu Ala 
        115                 120                 125 

Ile Thr Glu Gln Val Gln Ala Gly Glu Lys Thr Thr Glu Arg Leu Ile 
    130                 135                 140 

Trp Ala Gly Asn Thr Pro Gln Glu Lys Asp Tyr Asn Leu Ala Gly Gln 
145                 150                 155                 160 

Cys Val Arg His Tyr Asp Thr Ala Gly Leu Thr Gln Leu Asn Ser Leu 
                165                 170                 175 

Ser Leu Ala Gly Val Val Leu Ser Gln Ser Gln Gln Leu Leu Thr Asp 
            180                 185                 190 

Asn Gln Asp Ala Asp Trp Thr Gly Glu Asp Gln Ser Leu Trp Gln Gln 
        195                 200                 205 

Lys Leu Ser Ser Asp Val Tyr Ile Thr Gln Ser Asn Thr Asp Ala Thr 
    210                 215                 220 

Gly Ala Leu Leu Thr Gln Thr Asp Ala Lys Gly Asn Ile Gln Arg Leu 
225                 230                 235                 240 

Ala Tyr Asp Val Ala Gly Gln Leu Lys Gly Ser Trp Leu Thr Leu Lys 
                245                 250                 255 

Gly Gln Ala Glu Gln Val Ile Ile Lys Ser Leu Thr Tyr Ser Ala Ala 
            260                 265                 270 

Gly Gln Lys Leu Arg Glu Glu His Gly Asn Gly Ile Val Thr Glu Tyr 
        275                 280                 285 

Ser Tyr Glu Pro Glu Thr Gln Arg Leu Ile Gly Ile Thr Thr Arg Arg 
    290                 295                 300 

Pro Ser Asp Ala Lys Val Leu Gln Asp Leu Arg Tyr Gln Tyr Asp Pro 
305                 310                 315                 320 

Val Gly Asn Val Ile Asn Ile Arg Asn Asp Ala Glu Ala Thr Arg Phe 
                325                 330                 335 

Trp Arg Asn Gln Lys Val Ala Pro Glu Asn Ser Tyr Thr Tyr Asp Ser 
            340                 345                 350 

Leu Tyr Gln Leu Ile Ser Ala Thr Gly Arg Glu Met Ala Asn Ile Gly 
        355                 360                 365 

Gln Gln Asn Asn Gln Leu Pro Ser Pro Ala Leu Pro Ser Asp Asn Asn 
    370                 375                 380 

Thr Tyr Thr Asn Tyr Thr Arg Ser Tyr Ser Tyr Asp His Ser Gly Asn 
385                 390                 395                 400 

Leu Thr Gln Ile Arg His Ser Ser Pro Ala Thr Gln Asn Asn Tyr Thr 
                405                 410                 415 

Val Ala Ile Thr Leu Ser Asn Arg Ser Asn Arg Gly Val Leu Ser Thr 
            420                 425                 430 

Leu Thr Thr Asp Pro Asn Gln Val Asp Thr Leu Phe Asp Ala Gly Gly 
        435                 440                 445 

His Gln Thr Ser Leu Leu Pro Gly Gln Thr Leu Ile Trp Thr Pro Arg 
    450                 455                 460 

Gly Glu Leu Lys Gln Val Asn Asn Gly Pro Gly Asn Glu Trp Tyr Arg 
465                 470                 475                 480 

Tyr Asp Ser Asn Gly Met Arg Gln Leu Lys Val Ser Glu Gln Pro Thr 
                485                 490                 495 

Gln Asn Thr Thr Gln Gln Gln Arg Val Ile Tyr Leu Pro Gly Leu Glu 
            500                 505                 510 

Leu Arg Thr Thr Gln Ser Asn Ala Thr Thr Thr Glu Glu Leu His Val 
        515                 520                 525 

Ile Thr Leu Gly Glu Ala Gly Arg Ala Gln Val Arg Val Leu His Trp 
    530                 535                 540 

Glu Ser Gly Lys Pro Glu Asp Val Asn Asn Asn Gln Leu Arg Tyr Ser 
545                 550                 555                 560 

Tyr Asp Asn Leu Ile Gly Ser Ser Gln Leu Glu Leu Asp Asn Gln Gly 
                565                 570                 575 

Gln Ile Ile Ser Glu Glu Glu Tyr Tyr Pro Phe Gly Gly Thr Ala Leu 
            580                 585                 590 

Trp Ala Ala Asn Ser Gln Thr Glu Ala Ser Tyr Lys Thr Ile Arg Tyr 
        595                 600                 605 

Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly Tyr Arg 
    610                 615                 620 

Tyr Tyr Gln Pro Trp Ala Gly Arg Trp Leu Ser Ala Asp Pro Ala Gly 
625                 630                 635                 640 

Thr Ile Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn Pro Val 
                645                 650                 655 

Ser Leu Gln Asp Glu Asn Gly Leu Ala Pro Glu Lys Gly Lys Tyr Thr 
            660                 665                 670 

Lys Glu Val Asn Phe Phe Asp Glu Leu Lys Phe Lys Leu Ala Ala Lys 
        675                 680                 685 

Ser Ser His Val Val Lys Trp Asn Glu Lys Glu Ser Ser Tyr Thr Lys 
    690                 695                 700 

Asn Lys Ser Leu Lys Val Val Arg Val Gly Asp Ser Asp Pro Ser Gly 
705                 710                 715                 720 

Tyr Leu Leu Ser His Glu Glu Leu Leu Lys Gly Ile Glu Lys Ser Gln 
                725                 730                 735 

Ile Ile Tyr Ser Arg Leu Glu Glu Asn Ser Ser Leu Ser Glu Lys Ser 
            740                 745                 750 

Lys Thr Asn Leu Ser Leu Gly Ser Glu Ile Ser Gly Tyr Met Ala Arg 
        755                 760                 765 

Thr Ile Gln Asp Thr Ile Ser Glu Tyr Ala Glu Glu His Lys Tyr Arg 
    770                 775                 780 

Ser Asn His Pro Asp Phe Tyr Ser Glu Thr Asp Phe Phe Ala Leu Met 
785                 790                 795                 800 

Asp Lys Ser Glu Lys Asn Asp Tyr Ser Gly Glu Arg Lys Ile Tyr Ala 
                805                 810                 815 

Ala Met Glu Val Lys Val Tyr His Asp Leu Lys Asn Lys Gln Ser Glu 
            820                 825                 830 

Leu His Val Asn Tyr Ala Leu Ala His Pro Tyr Thr Gln Leu Ser Asn 
        835                 840                 845 

Glu Glu Arg Ala Leu Leu Gln Glu Thr Glu Pro Ala Ile Ala Ile Asp 
    850                 855                 860 

Arg Glu Tyr Asn Phe Lys Gly Val Gly Lys Phe Leu Thr Met Lys Ala 
865                 870                 875                 880 

Ile Lys Lys Ser Leu Lys Gly His Lys Ile Asn Arg Ile Ser Thr Glu 
                885                 890                 895 

Ala Ile Asn Ile Arg Ser Ala Ala Ile Ala Glu Asn Leu Gly Met Arg 
            900                 905                 910 

Arg Thr Ser 
        915 

 
           
             7  
             7512  
             DNA  
             Photorhabdus luminescens  
           
            7 

atgcaaaact cattatcaag cactatcgat actatttgtc agaaactgca attaacttgt     60 

ccggcggaaa ttgctttgta tccctttgat actttccggg aaaaaactcg gggaatggtt    120 

aattgggggg aagcaaaacg gatttatgaa attgcacaag cggaacagga tagaaaccta    180 

cttcatgaaa aacgtatttt tgcctatgct aatccgctgc tgaaaaacgc tgttcggttg    240 

ggtacccggc aaatgttggg ttttatacaa ggttatagtg atctgtttgg taatcgtgct    300 

gataactatg ccgcgccggg ctcggttgca tcgatgttct caccggcggc ttatttgacg    360 

gaattgtacc gtgaagccaa aaacttgcat gacagcagct caatttatta cctagataaa    420 

cgtcgcccgg atttagcaag cttaatgctc agccagaaaa atatggatga ggaaatttca    480 

acgctggctc tctctaatga attgtgcctt gccgggatcg aaacaaaaac aggaaaatca    540 

caagatgaag tgatggatat gttgtcaact tatcgtttaa gtggagagac accttatcat    600 

cacgcttatg aaactgttcg tgaaatcgtt catgaacgtg atccaggatt tcgtcatttg    660 

tcacaggcac ccattgttgc tgctaagctc gatcctgtga ctttgttggg tattagctcc    720 

catatttcgc cagaactgta taacttgctg attgaggaga tcccggaaaa agatgaagcc    780 

gcgcttgata cgctttataa aacaaacttt ggcgatatta ctactgctca gttaatgtcc    840 

ccaagttatc tggcccggta ttatggcgtc tcaccggaag atattgccta cgtgacgact    900 

tcattatcac atgttggata tagcagtgat attctggtta ttccgttggt cgatggtgtg    960 

ggtaagatgg aagtagttcg tgttacccga acaccatcgg ataattatac cagtcagacg   1020 

aattatattg agctgtatcc acagggtggc gacaattatt tgatcaaata caatctaagc   1080 

aatagttttg gtttggatga tttttatctg caatataaag atggttccgc tgattggact   1140 

gagattgccc ataatcccta tcctgatatg gtcataaatc aaaagtatga atcacaggcg   1200 

acaatcaaac gtagtgactc tgacaatata ctcagtatag ggttacaaag atggcatagc   1260 

ggtagttata attttgccgc cgccaatttt aaaattgacc aatactcccc gaaagctttc   1320 

ctgcttaaaa tgaataaggc tattcggttg ctcaaagcta ccggcctctc ttttgctacg   1380 

ttggagcgta ttgttgatag tgttaatagc accaaatcca tcacggttga ggtattaaac   1440 

aaggtttatc gggtaaaatt ctatattgat cgttatggca tcagtgaaga gacagccgct   1500 

attttggcta atattaatat ctctcagcaa gctgttggca atcagcttag ccagtttgag   1560 

caactattta atcacccgcc gctcaatggt attcgctatg aaatcagtga ggacaactcc   1620 

aaacatcttc ctaatcctga tctgaacctt aaaccagaca gtaccggtga tgatcaacgc   1680 

aaggcggttt taaaacgcgc gtttcaggtt aacgccagtg agttgtatca gatgttattg   1740 

atcactgatc gtaaagaaga cggtgttatc aaaaataact tagagaattt gtctgatctg   1800 

tatttggtta gtttgctggc ccagattcat aacctgacta ttgctgaatt gaacattttg   1860 

ttggtgattt gtggctatgg cgacaccaac atttatcaga ttaccgacga taatttagcc   1920 

aaaatagtgg aaacattgtt gtggatcact caatggttga agacccaaaa atggacagtt   1980 

accgacctgt ttctgatgac cacggccact tacagcacca ctttaacgcc agaaattagc   2040 

aatctgacgg ctacgttgtc ttcaactttg catggcaaag agagtctgat tggggaagat   2100 

ctgaaaagag caatggcgcc ttgcttcact tcggctttgc atttgacttc tcaagaagtt   2160 

gcgtatgacc tgctgttgtg gatagaccag attcaaccgg cacaaataac tgttgatggg   2220 

ttttgggaag aagtgcaaac aacaccaacc agcttgaagg tgattacctt tgctcaggtg   2280 

ctggcacaat tgagcctgat ctatcgtcgt attgggttaa gtgaaacgga actgtcactg   2340 

atcgtgactc aatcttctct gctagtggca ggcaaaagca tactggatca cggtctgtta   2400 

accctgatgg ccttggaagg ttttcatacc tgggttaatg gcttggggca acatgcctcc   2460 

ttgatattgg cggcgttgaa agacggagcc ttgacagtta ccgatgtagc acaagctatg   2520 

aataaggagg aatctctcct acaaatggca gctaatcagg tggagaagga tctaacaaaa   2580 

ctgaccagtt ggacacagat tgacgctatt ctgcaatggt tacagatgtc ttcggccttg   2640 

gcggtttctc cactggatct ggcagggatg atggccctga aatatgggat agatcataac   2700 

tatgctgcct ggcaagctgc ggcggctgcg ctgatggctg atcatgctaa tcaggcacag   2760 

aaaaaactgg atgagacgtt cagtaaggca ttatgtaact attatattaa tgctgttgtc   2820 

gatagtgctg ctggagtacg tgatcgtaac ggtttatata cctatttgct gattgataat   2880 

caggtttctg ccgatgtgat cacttcacgt attgcagaag ctatcgccgg tattcaactg   2940 

tacgttaacc gggctttaaa ccgagatgaa ggtcagcttg catcggacgt tagtacccgt   3000 

cagttcttca ctgactggga acgttacaat aaacgttaca gtacttgggc tggtgtctct   3060 

gaactggtct attatccaga aaactatgtt gatcccactc agcgcattgg gcaaaccaaa   3120 

atgatggatg cgctgttgca atccatcaac cagagccagc taaatgcgga tacggtggaa   3180 

gatgctttca aaacttattt gaccagcttt gagcaggtag caaatctgaa agtaattagt   3240 

gcttaccacg ataatgtgaa tgtggatcaa ggattaactt attttatcgg tatcgaccaa   3300 

gcagctccgg gtacgtatta ctggcgtagt gttgatcaca gcaaatgtga aaatggcaag   3360 

tttgccgcta atgcttgggg tgagtggaat aaaattacct gtgctgtcaa tccttggaaa   3420 

aatatcatcc gtccggttgt ttatatgtcc cgcttatatc tgctatggct ggagcagcaa   3480 

tcaaagaaaa gtgatgatgg taaaaccacg atttatcaat ataacttaaa actggctcat   3540 

attcgttacg acggtagttg gaatacacca tttacttttg atgtgacaga aaaggtaaaa   3600 

aattacacgt cgagtactga tgctgctgaa tctttagggt tgtattgtac tggttatcaa   3660 

ggggaagaca ctctattagt tatgttctat tcgatgcaga gtagttatag ctcctatacc   3720 

gataataatg cgccggtcac tgggctatat attttcgctg atatgtcatc agacaatatg   3780 

acgaatgcac aagcaactaa ctattggaat aacagttatc cgcaatttga tactgtgatg   3840 

gcagatccgg atagcgacaa taaaaaagtc ataaccagaa gagttaataa ccgttatgcg   3900 

gaggattatg aaattccttc ctctgtgaca agtaacagta attattcttg gggtgatcac   3960 

agtttaacca tgctttatgg tggtagtgtt cctaatatta cttttgaatc ggcggcagaa   4020 

gatttaaggc tatctaccaa tatggcattg agtattattc ataatggata tgcgggaacc   4080 

cgccgtatac aatgtaatct tatgaaacaa tacgcttcat taggtgataa atttataatt   4140 

tatgattcat catttgatga tgcaaaccgt tttaatctgg tgccattgtt taaattcgga   4200 

aaagacgaga actcagatga tagtatttgt atatataatg aaaacccttc ctctgaagat   4260 

aagaagtggt atttttcttc gaaagatgac aataaaacag cggattataa tggtggaact   4320 

caatgtatag atgctggaac cagtaacaaa gatttttatt ataatctcca ggagattgaa   4380 

gtaattagtg ttactggtgg gtattggtcg agttataaaa tatccaaccc gattaatatc   4440 

aatacgggca ttgatagtgc taaagtaaaa gtcaccgtaa aagcgggtgg tgacgatcaa   4500 

atctttactg ctgataatag tacctatgtt cctcagcaac cggcacccag ttttgaggag   4560 

atgatttatc agttcaataa cctgacaata gattgtaaga atttaaattt catcgacaat   4620 

caggcacata ttgagattga tttcaccgct acggcacaag atggccgatt cttgggtgca   4680 

gaaactttta ttatcccggt aactaaaaaa gttctcggta ctgagaacgt gattgcgtta   4740 

tatagcgaaa ataacggtgt tcaatatatg caaattggcg catatcgtac ccgtttgaat   4800 

acgttattcg ctcaacagtt ggttagccgt gctaatcgtg gcattgatgc agtgctcagt   4860 

atggaaactc agaatattca ggaaccgcaa ttaggagcgg gcacatatgt gcagcttgtg   4920 

ttggataaat atgatgagtc tattcatggc actaataaaa gctttgctat tgaatatgtt   4980 

gatatattta aagagaacga tagttttgtg atttatcaag gagaacttag cgaaacaagt   5040 

caaactgttg tgaaagtttt cttatcctat tttatagagg cgactggaaa taagaaccac   5100 

ttatgggtac gtgctaaata ccaaaaggaa acgactgata agatcttgtt cgaccgtact   5160 

gatgagaaag atccgcacgg ttggtttctc agcgacgatc acaagacctt tagtggtctc   5220 

tcttccgcac aggcattaaa gaacgacagt gaaccgatgg atttctctgg cgccaatgct   5280 

ctctatttct gggaactgtt ctattacacg ccgatgatga tggctcatcg tttgttgcag   5340 

gaacagaatt ttgatgcggc gaaccattgg ttccgttatg tctggagtcc atccggttat   5400 

atcgttgatg gtaaaattgc tatctaccac tggaacgtgc gaccgctgga agaagacacc   5460 

agttggaatg cacaacaact ggactccacc gatccagatg ctgtagccca agatgatccg   5520 

atgcactaca aggtggctac ctttatggcg acgttggatc tgctaatggc ccgtggtgat   5580 

gctgcttacc gccagttaga gcgtgatacg ttggctgaag ctaaaatgtg gtatacacag   5640 

gcgcttaatc tgttgggtga tgagccacaa gtgatgctga gtacgacttg ggctaatcca   5700 

acattgggta atgctgcttc aaaaaccaca cagcaggttc gtcagcaagt gcttacccag   5760 

ttgcgtctca atagcagggt aaaaaccccg ttgctaggaa cagccaattc cctgaccgct   5820 

ttattcctgc cgcaggaaaa tagcaagctc aaaggctact ggcggacact ggcgcagcgt   5880 

atgtttaatt tacgtcataa tctgtcgatt gacggccagc cgctctcctt gccgctgtat   5940 

gctaaaccgg ctgatccaaa agctttactg agtgcggcgg tttcagcttc tcaaggggga   6000 

gccgacttgc cgaaggcgcc gctgactatt caccgcttcc ctcaaatgct agaaggggca   6060 

cggggcttgg ttaaccagct tatacagttc ggtagttcac tattggggta cagtgagcgt   6120 

caggatgcgg aagctatgag tcaactactg caaacccaag ccagcgagtt aatactgacc   6180 

agtattcgta tgcaggataa ccaattggca gagctggatt cggaaaaaac cgccttgcaa   6240 

gtctctttag ctggagtgca acaacggttt gacagctata gccaactgta tgaggagaac   6300 

atcaacgcag gtgagcagcg agcgctggcg ttacgctcag aatctgctat tgagtctcag   6360 

ggagcgcaga tttcccgtat ggcaggcgcg ggtgttgata tggcaccaaa tatcttcggc   6420 

ctggctgatg gcggcatgca ttatggtgct attgcctatg ccatcgctga cggtattgag   6480 

ttgagtgctt ctgccaagat ggttgatgcg gagaaagttg ctcagtcgga aatatatcgc   6540 

cgtcgccgtc aagaatggaa aattcagcgt gacaacgcac aagcggagat taaccagtta   6600 

aacgcgcaac tggaatcact gtctattcgc cgtgaagccg ctgaaatgca aaaagagtac   6660 

ctgaaaaccc agcaagctca ggcgcaggca caacttactt tcttaagaag caaattcagt   6720 

aatcaagcgt tatatagttg gttacgaggg cgtttgtcag gtatttattt ccagttctat   6780 

gacttggccg tatcacgttg cctgatggca gagcaatcct atcaatggga agctaatgat   6840 

aattccatta gctttgtcaa accgggtgca tggcaaggaa cttacgccgg cttattgtgt   6900 

ggagaagctt tgatacaaaa tctggcacaa atggaagagg catatctgaa atgggaatct   6960 

cgcgctttgg aagtagaacg cacggtttca ttggcagtgg tttatgattc actggaaggt   7020 

aatgatcgtt ttaatttagc ggaacaaata cctgcattat tggataaggg ggagggaaca   7080 

gcaggaacta aagaaaatgg gttatcattg gctaatgcta tcctgtcagc ttcggtcaaa   7140 

ttgtccgact tgaaactggg aacggattat ccagacagta tcgttggtag caacaaggtt   7200 

cgtcgtatta agcaaatcag tgtttcgcta cctgcattgg ttgggcctta tcaggatgtt   7260 

caggctatgc tcagctatgg tggcagtact caattgccga aaggttgttc agcgttggct   7320 

gtgtctcatg gtaccaatga tagtggtcag ttccagttgg atttcaatga cggcaaatac   7380 

ctgccatttg aaggtattgc tcttgatgat cagggtacac tgaatcttca atttccgaat   7440 

gctaccgaca agcagaaagc aatattgcaa actatgagcg atattatttt gcatattcgt   7500 

tataccatcc gt                                                       7512 

 
           
             8  
             2504  
             PRT  
             Photorhabdus luminescens  
           
            8 

Met Gln Asn Ser Leu Ser Ser Thr Ile Asp Thr Ile Cys Gln Lys Leu 
1               5                   10                  15 

Gln Leu Thr Cys Pro Ala Glu Ile Ala Leu Tyr Pro Phe Asp Thr Phe 
            20                  25                  30 

Arg Glu Lys Thr Arg Gly Met Val Asn Trp Gly Glu Ala Lys Arg Ile 
        35                  40                  45 

Tyr Glu Ile Ala Gln Ala Glu Gln Asp Arg Asn Leu Leu His Glu Lys 
    50                  55                  60 

Arg Ile Phe Ala Tyr Ala Asn Pro Leu Leu Lys Asn Ala Val Arg Leu 
65                  70                  75                  80 

Gly Thr Arg Gln Met Leu Gly Phe Ile Gln Gly Tyr Ser Asp Leu Phe 
                85                  90                  95 

Gly Asn Arg Ala Asp Asn Tyr Ala Ala Pro Gly Ser Val Ala Ser Met 
            100                 105                 110 

Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Lys Asn 
        115                 120                 125 

Leu His Asp Ser Ser Ser Ile Tyr Tyr Leu Asp Lys Arg Arg Pro Asp 
    130                 135                 140 

Leu Ala Ser Leu Met Leu Ser Gln Lys Asn Met Asp Glu Glu Ile Ser 
145                 150                 155                 160 

Thr Leu Ala Leu Ser Asn Glu Leu Cys Leu Ala Gly Ile Glu Thr Lys 
                165                 170                 175 

Thr Gly Lys Ser Gln Asp Glu Val Met Asp Met Leu Ser Thr Tyr Arg 
            180                 185                 190 

Leu Ser Gly Glu Thr Pro Tyr His His Ala Tyr Glu Thr Val Arg Glu 
        195                 200                 205 

Ile Val His Glu Arg Asp Pro Gly Phe Arg His Leu Ser Gln Ala Pro 
    210                 215                 220 

Ile Val Ala Ala Lys Leu Asp Pro Val Thr Leu Leu Gly Ile Ser Ser 
225                 230                 235                 240 

His Ile Ser Pro Glu Leu Tyr Asn Leu Leu Ile Glu Glu Ile Pro Glu 
                245                 250                 255 

Lys Asp Glu Ala Ala Leu Asp Thr Leu Tyr Lys Thr Asn Phe Gly Asp 
            260                 265                 270 

Ile Thr Thr Ala Gln Leu Met Ser Pro Ser Tyr Leu Ala Arg Tyr Tyr 
        275                 280                 285 

Gly Val Ser Pro Glu Asp Ile Ala Tyr Val Thr Thr Ser Leu Ser His 
    290                 295                 300 

Val Gly Tyr Ser Ser Asp Ile Leu Val Ile Pro Leu Val Asp Gly Val 
305                 310                 315                 320 

Gly Lys Met Glu Val Val Arg Val Thr Arg Thr Pro Ser Asp Asn Tyr 
                325                 330                 335 

Thr Ser Gln Thr Asn Tyr Ile Glu Leu Tyr Pro Gln Gly Gly Asp Asn 
            340                 345                 350 

Tyr Leu Ile Lys Tyr Asn Leu Ser Asn Ser Phe Gly Leu Asp Asp Phe 
        355                 360                 365 

Tyr Leu Gln Tyr Lys Asp Gly Ser Ala Asp Trp Thr Glu Ile Ala His 
    370                 375                 380 

Asn Pro Tyr Pro Asp Met Val Ile Asn Gln Lys Tyr Glu Ser Gln Ala 
385                 390                 395                 400 

Thr Ile Lys Arg Ser Asp Ser Asp Asn Ile Leu Ser Ile Gly Leu Gln 
                405                 410                 415 

Arg Trp His Ser Gly Ser Tyr Asn Phe Ala Ala Ala Asn Phe Lys Ile 
            420                 425                 430 

Asp Gln Tyr Ser Pro Lys Ala Phe Leu Leu Lys Met Asn Lys Ala Ile 
        435                 440                 445 

Arg Leu Leu Lys Ala Thr Gly Leu Ser Phe Ala Thr Leu Glu Arg Ile 
    450                 455                 460 

Val Asp Ser Val Asn Ser Thr Lys Ser Ile Thr Val Glu Val Leu Asn 
465                 470                 475                 480 

Lys Val Tyr Arg Val Lys Phe Tyr Ile Asp Arg Tyr Gly Ile Ser Glu 
                485                 490                 495 

Glu Thr Ala Ala Ile Leu Ala Asn Ile Asn Ile Ser Gln Gln Ala Val 
            500                 505                 510 

Gly Asn Gln Leu Ser Gln Phe Glu Gln Leu Phe Asn His Pro Pro Leu 
        515                 520                 525 

Asn Gly Ile Arg Tyr Glu Ile Ser Glu Asp Asn Ser Lys His Leu Pro 
    530                 535                 540 

Asn Pro Asp Leu Asn Leu Lys Pro Asp Ser Thr Gly Asp Asp Gln Arg 
545                 550                 555                 560 

Lys Ala Val Leu Lys Arg Ala Phe Gln Val Asn Ala Ser Glu Leu Tyr 
                565                 570                 575 

Gln Met Leu Leu Ile Thr Asp Arg Lys Glu Asp Gly Val Ile Lys Asn 
            580                 585                 590 

Asn Leu Glu Asn Leu Ser Asp Leu Tyr Leu Val Ser Leu Leu Ala Gln 
        595                 600                 605 

Ile His Asn Leu Thr Ile Ala Glu Leu Asn Ile Leu Leu Val Ile Cys 
    610                 615                 620 

Gly Tyr Gly Asp Thr Asn Ile Tyr Gln Ile Thr Asp Asp Asn Leu Ala 
625                 630                 635                 640 

Lys Ile Val Glu Thr Leu Leu Trp Ile Thr Gln Trp Leu Lys Thr Gln 
                645                 650                 655 

Lys Trp Thr Val Thr Asp Leu Phe Leu Met Thr Thr Ala Thr Tyr Ser 
            660                 665                 670 

Thr Thr Leu Thr Pro Glu Ile Ser Asn Leu Thr Ala Thr Leu Ser Ser 
        675                 680                 685 

Thr Leu His Gly Lys Glu Ser Leu Ile Gly Glu Asp Leu Lys Arg Ala 
    690                 695                 700 

Met Ala Pro Cys Phe Thr Ser Ala Leu His Leu Thr Ser Gln Glu Val 
705                 710                 715                 720 

Ala Tyr Asp Leu Leu Leu Trp Ile Asp Gln Ile Gln Pro Ala Gln Ile 
                725                 730                 735 

Thr Val Asp Gly Phe Trp Glu Glu Val Gln Thr Thr Pro Thr Ser Leu 
            740                 745                 750 

Lys Val Ile Thr Phe Ala Gln Val Leu Ala Gln Leu Ser Leu Ile Tyr 
        755                 760                 765 

Arg Arg Ile Gly Leu Ser Glu Thr Glu Leu Ser Leu Ile Val Thr Gln 
    770                 775                 780 

Ser Ser Leu Leu Val Ala Gly Lys Ser Ile Leu Asp His Gly Leu Leu 
785                 790                 795                 800 

Thr Leu Met Ala Leu Glu Gly Phe His Thr Trp Val Asn Gly Leu Gly 
                805                 810                 815 

Gln His Ala Ser Leu Ile Leu Ala Ala Leu Lys Asp Gly Ala Leu Thr 
            820                 825                 830 

Val Thr Asp Val Ala Gln Ala Met Asn Lys Glu Glu Ser Leu Leu Gln 
        835                 840                 845 

Met Ala Ala Asn Gln Val Glu Lys Asp Leu Thr Lys Leu Thr Ser Trp 
    850                 855                 860 

Thr Gln Ile Asp Ala Ile Leu Gln Trp Leu Gln Met Ser Ser Ala Leu 
865                 870                 875                 880 

Ala Val Ser Pro Leu Asp Leu Ala Gly Met Met Ala Leu Lys Tyr Gly 
                885                 890                 895 

Ile Asp His Asn Tyr Ala Ala Trp Gln Ala Ala Ala Ala Ala Leu Met 
            900                 905                 910 

Ala Asp His Ala Asn Gln Ala Gln Lys Lys Leu Asp Glu Thr Phe Ser 
        915                 920                 925 

Lys Ala Leu Cys Asn Tyr Tyr Ile Asn Ala Val Val Asp Ser Ala Ala 
    930                 935                 940 

Gly Val Arg Asp Arg Asn Gly Leu Tyr Thr Tyr Leu Leu Ile Asp Asn 
945                 950                 955                 960 

Gln Val Ser Ala Asp Val Ile Thr Ser Arg Ile Ala Glu Ala Ile Ala 
                965                 970                 975 

Gly Ile Gln Leu Tyr Val Asn Arg Ala Leu Asn Arg Asp Glu Gly Gln 
            980                 985                 990 

Leu Ala Ser Asp Val Ser Thr Arg  Gln Phe Phe Thr Asp  Trp Glu Arg 
        995                 1000                 1005 

Tyr Asn  Lys Arg Tyr Ser Thr  Trp Ala Gly Val Ser  Glu Leu Val 
    1010                 1015                 1020 

Tyr Tyr  Pro Glu Asn Tyr Val  Asp Pro Thr Gln Arg  Ile Gly Gln 
    1025                 1030                 1035 

Thr Lys  Met Met Asp Ala Leu  Leu Gln Ser Ile Asn  Gln Ser Gln 
    1040                 1045                 1050 

Leu Asn  Ala Asp Thr Val Glu  Asp Ala Phe Lys Thr  Tyr Leu Thr 
    1055                 1060                 1065 

Ser Phe  Glu Gln Val Ala Asn  Leu Lys Val Ile Ser  Ala Tyr His 
    1070                 1075                 1080 

Asp Asn  Val Asn Val Asp Gln  Gly Leu Thr Tyr Phe  Ile Gly Ile 
    1085                 1090                 1095 

Asp Gln  Ala Ala Pro Gly Thr  Tyr Tyr Trp Arg Ser  Val Asp His 
    1100                 1105                 1110 

Ser Lys  Cys Glu Asn Gly Lys  Phe Ala Ala Asn Ala  Trp Gly Glu 
    1115                 1120                 1125 

Trp Asn  Lys Ile Thr Cys Ala  Val Asn Pro Trp Lys  Asn Ile Ile 
    1130                 1135                 1140 

Arg Pro  Val Val Tyr Met Ser  Arg Leu Tyr Leu Leu  Trp Leu Glu 
    1145                 1150                 1155 

Gln Gln  Ser Lys Lys Ser Asp  Asp Gly Lys Thr Thr  Ile Tyr Gln 
    1160                 1165                 1170 

Tyr Asn  Leu Lys Leu Ala His  Ile Arg Tyr Asp Gly  Ser Trp Asn 
    1175                 1180                 1185 

Thr Pro  Phe Thr Phe Asp Val  Thr Glu Lys Val Lys  Asn Tyr Thr 
    1190                 1195                 1200 

Ser Ser  Thr Asp Ala Ala Glu  Ser Leu Gly Leu Tyr  Cys Thr Gly 
    1205                 1210                 1215 

Tyr Gln  Gly Glu Asp Thr Leu  Leu Val Met Phe Tyr  Ser Met Gln 
    1220                 1225                 1230 

Ser Ser  Tyr Ser Ser Tyr Thr  Asp Asn Asn Ala Pro  Val Thr Gly 
    1235                 1240                 1245 

Leu Tyr  Ile Phe Ala Asp Met  Ser Ser Asp Asn Met  Thr Asn Ala 
    1250                 1255                 1260 

Gln Ala  Thr Asn Tyr Trp Asn  Asn Ser Tyr Pro Gln  Phe Asp Thr 
    1265                 1270                 1275 

Val Met  Ala Asp Pro Asp Ser  Asp Asn Lys Lys Val  Ile Thr Arg 
    1280                 1285                 1290 

Arg Val  Asn Asn Arg Tyr Ala  Glu Asp Tyr Glu Ile  Pro Ser Ser 
    1295                 1300                 1305 

Val Thr  Ser Asn Ser Asn Tyr  Ser Trp Gly Asp His  Ser Leu Thr 
    1310                 1315                 1320 

Met Leu  Tyr Gly Gly Ser Val  Pro Asn Ile Thr Phe  Glu Ser Ala 
    1325                 1330                 1335 

Ala Glu  Asp Leu Arg Leu Ser  Thr Asn Met Ala Leu  Ser Ile Ile 
    1340                 1345                 1350 

His Asn  Gly Tyr Ala Gly Thr  Arg Arg Ile Gln Cys  Asn Leu Met 
    1355                 1360                 1365 

Lys Gln  Tyr Ala Ser Leu Gly  Asp Lys Phe Ile Ile  Tyr Asp Ser 
    1370                 1375                 1380 

Ser Phe  Asp Asp Ala Asn Arg  Phe Asn Leu Val Pro  Leu Phe Lys 
    1385                 1390                 1395 

Phe Gly  Lys Asp Glu Asn Ser  Asp Asp Ser Ile Cys  Ile Tyr Asn 
    1400                 1405                 1410 

Glu Asn  Pro Ser Ser Glu Asp  Lys Lys Trp Tyr Phe  Ser Ser Lys 
    1415                 1420                 1425 

Asp Asp  Asn Lys Thr Ala Asp  Tyr Asn Gly Gly Thr  Gln Cys Ile 
    1430                 1435                 1440 

Asp Ala  Gly Thr Ser Asn Lys  Asp Phe Tyr Tyr Asn  Leu Gln Glu 
    1445                 1450                 1455 

Ile Glu  Val Ile Ser Val Thr  Gly Gly Tyr Trp Ser  Ser Tyr Lys 
    1460                 1465                 1470 

Ile Ser  Asn Pro Ile Asn Ile  Asn Thr Gly Ile Asp  Ser Ala Lys 
    1475                 1480                 1485 

Val Lys  Val Thr Val Lys Ala  Gly Gly Asp Asp Gln  Ile Phe Thr 
    1490                 1495                 1500 

Ala Asp  Asn Ser Thr Tyr Val  Pro Gln Gln Pro Ala  Pro Ser Phe 
    1505                 1510                 1515 

Glu Glu  Met Ile Tyr Gln Phe  Asn Asn Leu Thr Ile  Asp Cys Lys 
    1520                 1525                 1530 

Asn Leu  Asn Phe Ile Asp Asn  Gln Ala His Ile Glu  Ile Asp Phe 
    1535                 1540                 1545 

Thr Ala  Thr Ala Gln Asp Gly  Arg Phe Leu Gly Ala  Glu Thr Phe 
    1550                 1555                 1560 

Ile Ile  Pro Val Thr Lys Lys  Val Leu Gly Thr Glu  Asn Val Ile 
    1565                 1570                 1575 

Ala Leu  Tyr Ser Glu Asn Asn  Gly Val Gln Tyr Met  Gln Ile Gly 
    1580                 1585                 1590 

Ala Tyr  Arg Thr Arg Leu Asn  Thr Leu Phe Ala Gln  Gln Leu Val 
    1595                 1600                 1605 

Ser Arg  Ala Asn Arg Gly Ile  Asp Ala Val Leu Ser  Met Glu Thr 
    1610                 1615                 1620 

Gln Asn  Ile Gln Glu Pro Gln  Leu Gly Ala Gly Thr  Tyr Val Gln 
    1625                 1630                 1635 

Leu Val  Leu Asp Lys Tyr Asp  Glu Ser Ile His Gly  Thr Asn Lys 
    1640                 1645                 1650 

Ser Phe  Ala Ile Glu Tyr Val  Asp Ile Phe Lys Glu  Asn Asp Ser 
    1655                 1660                 1665 

Phe Val  Ile Tyr Gln Gly Glu  Leu Ser Glu Thr Ser  Gln Thr Val 
    1670                 1675                 1680 

Val Lys  Val Phe Leu Ser Tyr  Phe Ile Glu Ala Thr  Gly Asn Lys 
    1685                 1690                 1695 

Asn His  Leu Trp Val Arg Ala  Lys Tyr Gln Lys Glu  Thr Thr Asp 
    1700                 1705                 1710 

Lys Ile  Leu Phe Asp Arg Thr  Asp Glu Lys Asp Pro  His Gly Trp 
    1715                 1720                 1725 

Phe Leu  Ser Asp Asp His Lys  Thr Phe Ser Gly Leu  Ser Ser Ala 
    1730                 1735                 1740 

Gln Ala  Leu Lys Asn Asp Ser  Glu Pro Met Asp Phe  Ser Gly Ala 
    1745                 1750                 1755 

Asn Ala  Leu Tyr Phe Trp Glu  Leu Phe Tyr Tyr Thr  Pro Met Met 
    1760                 1765                 1770 

Met Ala  His Arg Leu Leu Gln  Glu Gln Asn Phe Asp  Ala Ala Asn 
    1775                 1780                 1785 

His Trp  Phe Arg Tyr Val Trp  Ser Pro Ser Gly Tyr  Ile Val Asp 
    1790                 1795                 1800 

Gly Lys  Ile Ala Ile Tyr His  Trp Asn Val Arg Pro  Leu Glu Glu 
    1805                 1810                 1815 

Asp Thr  Ser Trp Asn Ala Gln  Gln Leu Asp Ser Thr  Asp Pro Asp 
    1820                 1825                 1830 

Ala Val  Ala Gln Asp Asp Pro  Met His Tyr Lys Val  Ala Thr Phe 
    1835                 1840                 1845 

Met Ala  Thr Leu Asp Leu Leu  Met Ala Arg Gly Asp  Ala Ala Tyr 
    1850                 1855                 1860 

Arg Gln  Leu Glu Arg Asp Thr  Leu Ala Glu Ala Lys  Met Trp Tyr 
    1865                 1870                 1875 

Thr Gln  Ala Leu Asn Leu Leu  Gly Asp Glu Pro Gln  Val Met Leu 
    1880                 1885                 1890 

Ser Thr  Thr Trp Ala Asn Pro  Thr Leu Gly Asn Ala  Ala Ser Lys 
    1895                 1900                 1905 

Thr Thr  Gln Gln Val Arg Gln  Gln Val Leu Thr Gln  Leu Arg Leu 
    1910                 1915                 1920 

Asn Ser  Arg Val Lys Thr Pro  Leu Leu Gly Thr Ala  Asn Ser Leu 
    1925                 1930                 1935 

Thr Ala  Leu Phe Leu Pro Gln  Glu Asn Ser Lys Leu  Lys Gly Tyr 
    1940                 1945                 1950 

Trp Arg  Thr Leu Ala Gln Arg  Met Phe Asn Leu Arg  His Asn Leu 
    1955                 1960                 1965 

Ser Ile  Asp Gly Gln Pro Leu  Ser Leu Pro Leu Tyr  Ala Lys Pro 
    1970                 1975                 1980 

Ala Asp  Pro Lys Ala Leu Leu  Ser Ala Ala Val Ser  Ala Ser Gln 
    1985                 1990                 1995 

Gly Gly  Ala Asp Leu Pro Lys  Ala Pro Leu Thr Ile  His Arg Phe 
    2000                 2005                 2010 

Pro Gln  Met Leu Glu Gly Ala  Arg Gly Leu Val Asn  Gln Leu Ile 
    2015                 2020                 2025 

Gln Phe  Gly Ser Ser Leu Leu  Gly Tyr Ser Glu Arg  Gln Asp Ala 
    2030                 2035                 2040 

Glu Ala  Met Ser Gln Leu Leu  Gln Thr Gln Ala Ser  Glu Leu Ile 
    2045                 2050                 2055 

Leu Thr  Ser Ile Arg Met Gln  Asp Asn Gln Leu Ala  Glu Leu Asp 
    2060                 2065                 2070 

Ser Glu  Lys Thr Ala Leu Gln  Val Ser Leu Ala Gly  Val Gln Gln 
    2075                 2080                 2085 

Arg Phe  Asp Ser Tyr Ser Gln  Leu Tyr Glu Glu Asn  Ile Asn Ala 
    2090                 2095                 2100 

Gly Glu  Gln Arg Ala Leu Ala  Leu Arg Ser Glu Ser  Ala Ile Glu 
    2105                 2110                 2115 

Ser Gln  Gly Ala Gln Ile Ser  Arg Met Ala Gly Ala  Gly Val Asp 
    2120                 2125                 2130 

Met Ala  Pro Asn Ile Phe Gly  Leu Ala Asp Gly Gly  Met His Tyr 
    2135                 2140                 2145 

Gly Ala  Ile Ala Tyr Ala Ile  Ala Asp Gly Ile Glu  Leu Ser Ala 
    2150                 2155                 2160 

Ser Ala  Lys Met Val Asp Ala  Glu Lys Val Ala Gln  Ser Glu Ile 
    2165                 2170                 2175 

Tyr Arg  Arg Arg Arg Gln Glu  Trp Lys Ile Gln Arg  Asp Asn Ala 
    2180                 2185                 2190 

Gln Ala  Glu Ile Asn Gln Leu  Asn Ala Gln Leu Glu  Ser Leu Ser 
    2195                 2200                 2205 

Ile Arg  Arg Glu Ala Ala Glu  Met Gln Lys Glu Tyr  Leu Lys Thr 
    2210                 2215                 2220 

Gln Gln  Ala Gln Ala Gln Ala  Gln Leu Thr Phe Leu  Arg Ser Lys 
    2225                 2230                 2235 

Phe Ser  Asn Gln Ala Leu Tyr  Ser Trp Leu Arg Gly  Arg Leu Ser 
    2240                 2245                 2250 

Gly Ile  Tyr Phe Gln Phe Tyr  Asp Leu Ala Val Ser  Arg Cys Leu 
    2255                 2260                 2265 

Met Ala  Glu Gln Ser Tyr Gln  Trp Glu Ala Asn Asp  Asn Ser Ile 
    2270                 2275                 2280 

Ser Phe  Val Lys Pro Gly Ala  Trp Gln Gly Thr Tyr  Ala Gly Leu 
    2285                 2290                 2295 

Leu Cys  Gly Glu Ala Leu Ile  Gln Asn Leu Ala Gln  Met Glu Glu 
    2300                 2305                 2310 

Ala Tyr  Leu Lys Trp Glu Ser  Arg Ala Leu Glu Val  Glu Arg Thr 
    2315                 2320                 2325 

Val Ser  Leu Ala Val Val Tyr  Asp Ser Leu Glu Gly  Asn Asp Arg 
    2330                 2335                 2340 

Phe Asn  Leu Ala Glu Gln Ile  Pro Ala Leu Leu Asp  Lys Gly Glu 
    2345                 2350                 2355 

Gly Thr  Ala Gly Thr Lys Glu  Asn Gly Leu Ser Leu  Ala Asn Ala 
    2360                 2365                 2370 

Ile Leu  Ser Ala Ser Val Lys  Leu Ser Asp Leu Lys  Leu Gly Thr 
    2375                 2380                 2385 

Asp Tyr  Pro Asp Ser Ile Val  Gly Ser Asn Lys Val  Arg Arg Ile 
    2390                 2395                 2400 

Lys Gln  Ile Ser Val Ser Leu  Pro Ala Leu Val Gly  Pro Tyr Gln 
    2405                 2410                 2415 

Asp Val  Gln Ala Met Leu Ser  Tyr Gly Gly Ser Thr  Gln Leu Pro 
    2420                 2425                 2430 

Lys Gly  Cys Ser Ala Leu Ala  Val Ser His Gly Thr  Asn Asp Ser 
    2435                 2440                 2445 

Gly Gln  Phe Gln Leu Asp Phe  Asn Asp Gly Lys Tyr  Leu Pro Phe 
    2450                 2455                 2460 

Glu Gly  Ile Ala Leu Asp Asp  Gln Gly Thr Leu Asn  Leu Gln Phe 
    2465                 2470                 2475 

Pro Asn  Ala Thr Asp Lys Gln  Lys Ala Ile Leu Gln  Thr Met Ser 
    2480                 2485                 2490 

Asp Ile  Ile Leu His Ile Arg  Tyr Thr Ile Arg 
    2495                 2500