Abstract:
An apparatus for photometrically testing several specimens each irradiated by a light source, the light altered by the specimens being detected by an optical device and analyzed, the apparatus including a light source, a plurality of sample holders configured adjacent to one another on a support, a detector that receives altered light from sample within the sample holders, the detector including a filter for eliminating interfering light, a sensor having a sensor face and a diffusing optical member located between the filter and the sensor, wherein light is diffused and shines on a greater portion of the sensor surface, and with a more homogeneous brightness as compared to when the light is not diffused.

Description:
BACKGROUND OF THE INVENTION 
       [0001]    A device for the photometric measurement of samples is generally disclosed within U.S. Pat. No. 7,102,131 to Spolaczyk et al. Samples within individual sample wells are exposed to radiation from a single light source or a series of light sources and the light modified by the samples, for example fluorescence light, is intercepted by an optical device and is guided to a section of at least one sensor for measuring the intensity and evaluation thereof in an evaluation device arranged downstream of the sensor. The light source(s) are controlled individually by a control device, and the evaluation device and the control device are controlled such that the evaluation device can separate the light from each sample from the light of the other samples. The position of the sample upon, for example, a thermocycler that supports the sample determines the exact position where light modified by the samples shines on the sensor. Small shifts in the position of the sample will slightly shift the area on the face of the sensor where the light from the sample shines. 
         [0002]    The inventors of the present invention, through testing have determined that the sensor inherently has different sensitivity levels across its face. Additionally, the sensor is subject to the effect of intensity drift over time. Also, the magnitude of intensity drift varies across the face of the sensor. As a result of these three factors, when the position on the sensor where light from the sample shines changes, measurement results can be difficult to reproduce with good precision. Additionally, if the brightness of the light is not consistent across the section of the sensor upon which the light shines, measurement results are difficult to reproduce. Variations in brightness may be caused by the variations in position of the sample material in sample wells. 
         [0003]    It is possible to take the different sensitivity of the sensor area into consideration for every location of the specimens by calibration and determining correction factors, and calculating corrected measurement results with the correction factors. This is a time consuming process and also cannot be used for correcting intensity drift. 
         [0004]    What is desired is a way to quickly and easily increase the homogeneity of the sensing qualities of the sensor and as a result improve the precision and reproduceability of measurements recorded from tested samples. 
       BRIEF SUMMARY OF THE INVENTION 
       [0005]    An apparatus for photometrically testing several specimens, each irradiated by a light source, and the light altered by the specimens being detected by an optical device, is provided. The apparatus includes plurality of light sources (each associated with a specimen) or only a single light source, a plurality of sample holders or wells configured adjacent to one another on a support (preferably a thermocycler), a detector that receives altered light from sample within the sample holders, a filter for eliminating interfering light, and a sensor having a sensor face. The detector also includes a light diffusing member (light diffuser), wherein light from a sample is diffused and shines on a greater portion of the sensor face surface as compared to when the light is not diffused. 
         [0006]    The filter eliminating interfering light (wavelength filter) is situated in front of the sensor. As regards fluorescence measurements, the filter is a long wavelength filter eliminating residual short wavelength portions of the excitation light illustratively arising as scattered light from the light sources. Bandpass filters are also appropriate for these purposes. 
         [0007]    A bundle of optic fibers receives light from the samples and subsequently emits the light. The diffusing optical member is placed between the end of the fiber optic bundle, which first receives the light entering the detector, and the sensor. Preferably, the diffusing optical member is placed directly behind the wavelength filter. The wavelength filter, preferably, is supported in a structure that has the form of a wheel and the diffusing optical member is configured within a circular frame that is screwed to the wavelength filter support structure. 
         [0008]    As a result of the diffusing optical member, a sample light beam is placed on a larger percentage of the area of the sensor face and more homogeneous illumination of the sensor is provided. The effects of intensity drift, non-uniform sensitivity across the face of the sensor, and non-uniform intensity drift across the face of the sensor are significantly reduced. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0009]      FIG. 1  shows a schematic view of a device for photometric measurement of several samples including a diffusing optical member; 
           [0010]      FIG. 2  is a perspective view of light passing through optical elements of the device without utilizing the diffusing optical member; 
           [0011]      FIG. 3  is a perspective view of light from a different sample well passing through optical elements of the device without utilizing the diffusing optical member; 
           [0012]      FIG. 4  is a perspective view of light passing through optical elements of the device while utilizing the diffusing optical member; and 
           [0013]      FIG. 5  is a perspective view of a wavelength filter wheel support structure including the diffusing optical member. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0014]    The specification of U.S. Pat. No. 7,102,131 to Spolaczyk et al., which issued on Sep. 5, 2006, is hereby incorporated in its entirety by reference. 
         [0015]    Referring to  FIG. 1 , the apparatus  10  comprises a schematic, conventional thermocycler  11  with wells  12 . Reaction vials (not shown) are in place in the wells  12 . Each vial contains one sample with one or more fluorescence indicators. 
         [0016]    A covered housing  13  fitted with an illumination unit of several LEDs  14  is set on the thermocycler  11 . One LED  14  is allocated to each well  12 . Preferably, the LEDs  14  are configured as an array. Each LED  14  points in a direction such that it will irradiate only one associated well  12  and, if possible at all, not the adjacent wells. The LEDs  14  may, in particular, be laser diodes. The LEDs  14  are connected by cables  30  to a control unit  31  which is in turn connected by a cable  33  to an analyzer  32 . Alternatively, a single source of light may be used. 
         [0017]    An illustrative light path is denoted by  15 ,  15 ′. The light  15  is radiated from the LED  14  and first passes through an optional, but preferred, short wavelength filter  16  by means of which long wavelength components are filtered out. Then the light  15  passes through a beam splitter  17 , which, in this instance, preferably shall be wholly transmitting. 
         [0018]    The light  15  radiated from the LED  14  will excite a fluorescence indicator contained in a sample in the well  12 . The indicator, in turn, emits a fluorescence signal  15 ′. The beam splitter  17  is designed such that the fluorescence signal  15 ′ is reflected laterally. A dichroic mirror is preferably used as the beam splitter  17  and will transmit the excitation light while reflecting the emitted, longer wavelength fluorescence signal. 
         [0019]    The reflected fluorescence signal  15 ′ is then sensed by a detector  18 . The detector  18  is fitted with an optical device  19  that can reproduce the fluorescence signal  15 ′ onto a light sensitive sensor  27 . 
         [0020]    In lieu of the typical large-area lens element, the collimator in the optical device  19  is an array of optic fibers  20 . The optical fiber array catches the light from the wells  12  reflected by the beam splitter  17  by means of the mutually spaced light input areas  21  and harnessing optic fibers  20  so as to transmit the light through parallel harness ends at  23 . 
         [0021]    Contrary to a collimator composed of lens elements, the collimator configuration of a bundle of optic fibers  20  offers the feature that the light exiting the bundle optic fibers at  23  is collimated more narrowly. That feature is especially advantageous when, for instance, interference filters offering a spectral transfer function that depends on the light&#39;s angle of incidence are configured subsequently. 
         [0022]    Next, the fluorescence signal  15 ′ is reproduced through the optic fibers harness  23 , through a lens element  24 , through a long wavelength filter  25 , and a further lens element  26  onto a sensor  27 , preferably a channel photo multiplier. The long wave pass filter  25  is required in order to filter any shorter wavelength regions out of the excitation light. 
         [0023]    Referring to  FIGS. 1 and 4 , additionally, a diffusing optical member  40  is placed between the end of the fiber optical bundle  23  and the sensor  27 . The diffusing optical member  40  is for example a “light shaping diffuser” sold by Physical Optics Corporation of Torrance, Calif. The diffusing optical member  40  is preferably in the form of a thin panel, having a thickness of approximately 0.5 millimeters or less. The diffusing optical member  40  transmits at least 70% of the altered light applied thereto and preferably at least 90% of the altered light. The diffusing optical member  40  has a predetermined angular distribution so as to illuminate a large predefined area  36  of the sensor face  34 . The exact position of the focus area  36  on the sensor face  34  depends on the location of the specimen on the thermocycler  11  or the sample within a sample well. Different locations cause slight variations in the position of the focus area  36 . 
         [0024]    Preferably, the diffusing optical member  40  is arranged directly behind the long wavelength filter  25 , although the diffusing optical member  40  may be located anywhere between the optic fiber bundle  23  and the sensor  27 . Referring to  FIG. 5 , in one embodiment of the invention, the diffusing optical member  40  is secured to a circular frame  42  and the frame  42 , in turn, is screwed to the long wavelength filter support structure, directly adjacent to the wavelength filter  25 . The long wavelength filter  25 , preferably is one of a group of filters supported within the rotatable wheel-like support structure  50 . Each different filter  25  in the group has different filtering properties and can easily be placed into the optical path by rotating the wheel like structure  50 . Alternatively, a large diffusing optical member may be used that optically covers the whole wheel-like structure  50 . The holographic area of the diffusing optical member  40  is placed in the direction of the wavelength filter  25  and as a result effects a reduction of transmission loss due to lowered reflections on the surface of the diffusing optical member  40 . 
         [0025]    Referring to  FIG. 4 , the diffusing optical member  40  diffuses a beam of light, so that a larger portion of the sensor (compared to a system without a diffusing optical member) is exposed to the reflected light of the sample. 
         [0026]    Because the sensor face predefined focus area  36 , exposed to light from the sample, is larger when using the diffusing optical member, the effects of (1) varying sensitivity across the sensor face  34 , (2) intensity drift and (3) varying intensity drift across the sensor face  34 , are mitigated. 
         [0027]    Additionally, any positional shifts due to different sample locations are mitigated. For example, referring to  FIG. 2 , light from a first sample is shown on the sensor face  34 . Referring to  FIG. 3 , light from a second sample, placed in the identical well  12  on the thermocycler, but in a different position, or in an adjacent well, may shine on the sensor face  34  in a slightly different position (original position shown in broken lines). Because of the effects of variations in sensitivity, intensity drift, and variations in intensity drift, sensor readings made from these two adjacent positions will likely not be the same when testing identical samples. However, referring to  FIG. 4 , where the light reflected from the sample is diffused, virtually the same area of the sensor is covered, no matter what variations there are in sample wells or in the position of the sample within the sample well  12 . Thus, the effects of sensitivity, intensity drift, and variations in intensity drift, are mitigated. 
         [0028]    Additionally, the diffusing optical member  40  creates fewer variations in brightness across the focus area  36  on the sensor which also mitigates the effects of the three factors listed above. 
         [0029]    As an alternative embodiment of the invention, the diffusing optical member may also be provided in the form of a film that is applied to the surface of the wavelength filter  25 . The diffusing optical member may be mounted in front of the lens element  26  which is a collecting lens. 
         [0030]    Although the invention has been shown and described with reference to certain preferred and alternate embodiments, the invention is not limited to these specific embodiments. Minor variations and insubstantial differences in the various combinations of materials and methods of application may occur to those of ordinary skill in the art while remaining within the scope of the invention as claimed and equivalents.