Abstract:
The major histocompatibility complex (MHC) of domesticated fowl, the B system, is known to contain three subregions which are identified as  B-F ,  B-G  and  B-L . This invention includes a cDNA clone encoding a B-G antigen of the B system. MHC haplotyping is accomplished by use of novel probes provided by clones to detect restriction fragment length polymorphism (RFLP) patterns typical for various B-G subregion alleles. 
     Additional information concerning this invention is set forth in the attached manuscript entitled “Hypervariable sequence diversity in Ig V-like and leucine heptad domains in chicken histocompatibility B-G antigens”.

Description:
This is a division of application Ser. No. 07/865,662 filed Apr. 7, 1992, issued as U.S. Pat. No. 5,451,670, which is a continuation of application Ser. No. 07/688,326 filed Apr. 22, 1991 (now abandoned), which is a continuation-in-part of Ser. No. 07/588,922 filed Sep. 27, 1990 now abandoned, which is a continuation-in-part of Ser. No. 07/210,405 filed Jun. 23, 1988 now abandoned, which is a continuation-in-part of Ser. No. 07/130,529 filed Dec. 9, 1987 (now abandoned), which is a continuation-in-part of Ser. No. 07/068,176 filed Jun. 30, 1987 (now abandoned) and which is a continuation-in-part of Ser. No. 07/413,301 filed Sep. 28, 1989 (now abandoned). 
     Each of applications Ser. Nos. 210,405; 130,529; 068,176; 413,301, and 588,922 is incorporated herein by reference. 
    
    
     FIELD OF THE INVENTION 
     This invention relates to restriction fragment length polymorphism pattern tests useful to genotype domesticated fowl for the major histocompatibility B-G loci. The invention also relates to the use of certain B-G polypeptides to impart immunity to or to control the susceptibility of domesticated fowl to various diseases. 
     BACKGROUND OF THE INVENTION 
     In domesticated fowl the major histocompatibility complex (MHC) which is associated with the regulation of immune recognition and immune response is called the  B  system. Resistance to Marek&#39;s disease is closely related to the domesticated fowl MHC. Resistance to other diseases, general fitness, and productivity also appear to be influenced to some extent by MHC haplotype. 
     MHC haplotyping of chickens is presently done by hemagglutination assay which relies on the production of specific antisera. The assay in itself is technically simple. However, the production of the antisera and the interpretation of the assays require a highly trained individual. The MHC haplotypes present in commercial strains of chickens are usually a trade secret known only to individual breeders. Isolation of cloned gene sequences from the  B  system provides a means of developing alternative methods for MHC haplotyping of birds and for determining the genotype at particular loci within the  B  system. The interpretation of results is generally simpler and more uniform since typing by restriction fragment length polymorphism patterns is no longer dependent upon alloantisera which often require selective absorptions with blood samples from genetically-defined animals to delineate haplotype specificity. 
     SUMMARY OF THE INVENTION 
     The  B  system of histocompatibility in domesticated fowl is known to contain three subregions which are identified as  B-F ,  B-G  and  B-L .  B-F ,  B-G  and  B-L  are described as subregions because multiple genes of each type are present within the region of the  B  system. This invention includes cDNA clones encoding  B-G  antigens of the  B  system. MHC haplotyping is accomplished by use of novel probes provided by these clones to detect restriction fragment length polymorphism (RFLP) patterns typical for various  B-G  alleles present at the multiple loci within the B-G subregion. 
     Genetic recombination within the  B  system of the chicken is rare. For that reason, while the probes of this invention screen for the  B-G  genes, additional genes also of importance to disease resistance may be located in regions within and closely adjacent to the  B  system and genetically and physically linked to the  B-G  type. Other genes of mostly unknown function are located within the MHC as well. 
    
    
     DESCRIPTION OF THE FIGURES 
     FIG.  1 . Immunoblot of B-G21 antigen and λbg28 lysogen proteins reacted with antibodies specific to the bg28-β-galactosidase fusion protein. 
     A. Coomassie-blue stained SDS-8% polyacrylamide gel containing the following protein samples: 1 μg purified B-G21 antigen (lane 1); 40 μg of total cell protein from a λbg28 lysogen grown in the presence of IPTG (lane 2); 40 μg of total cell protein from a λgt11 lysogen grown in the presence of IPTG (lane 3); 40 μg of total cell protein from λbg28 lysogen grown in the absence of IPTG (lane 4); and protein size markers (marked MK) with their respective molecular weights given to the left in kilodaltons (kDa). 
     B. Parallel immunoblot. The same protein samples were subjected to SDS-polyacrylamide gel electrophoresis as in FIG.  1 A and then were electrophoretically transferred to a hybridization membrane. The proteins were reacted with B-G antigen-directed antiserum that had been affinity purified against bg28-β-galactosidase fusion protein. Bound antibodies were detected with  125 I-Protein A and the above autoradiogram was the result of an overnight exposure with an intensifying screen at −70° C. The white arrowheads mark the position of the bg28-β-galactosidase fusion protein. The dark arrowheads mark the positions of the two polypeptides of B-G21 antigen. 
     FIG.  2 . Northern analyses of poly(A) +  RNA from embryonic tissues. Poly(A) +  RNA samples (1 μg each) from the brain (BR), gizzard (GI), and erythrocytes  B  (ER) were subjected to formaldehyde agarose gel electrophoresis, transferred to a hybridization membrane, and hybridized with either  32 P-labeled bg28 insert (A) or a  32 P-labeled β-actin probe (B). The autoradiogram shown in (A) was the result of a 16-hour exposure the autoradiogram shown in (B) was the result of a 1-hour exposure. A 16-hour exposure of (B) revealed an actin MRNA species in the erythrocyte RNA sample (data not shown). 
     FIG.  3 . Southern analyses of chicken genomic DNA from birds disomic, trisomic, or tetrasomic for the  B  system-bearing microchromosome.  Pvu II-digested genomic DNA (5 μg each) from chickens either disomic (2×), trisomic (3×), or tetrasomic (4×) for the  B -complex microchromosome were subjected to electrophoresis on an 0.8% agarose gel and hybridized within the gel to either  32 P-labeled λbg28 insert (left 4 samples) or a  32 P-labeled β-actin probe (right 3 samples). The lane marked C contained 10 pg of  Hind III-linearized Bluescript plasmid containing the bg28 insert. on the left are molecular size markers (in kilobase pairs) based on a  Hind III digest of phage λ. The above autoradiograms were the result of an overnight exposure. 
     FIG.  4 . Hybridization of the bg28 insert to restriction digests of chicken genomic DNA from birds of different  B  haplotypes.  Pvu II-digested genomic DNA (5 μg each) from chickens of different  B  haplotypes were subjected to electrophoresis on an 0.8% agarose gel and hybridized within the gel to  32 P-labeled bg28 insert. DNA samples are labeled according to their respective  B  haplotype (see Table 1). The lane marked C contained 10 pg of  Hind III-linearized Bluescript plasmid containing the bg28 insert. On the left are molecular size markers (in kilobase pairs) based on a  Hind III digest of phage λ. The above autoradiogram was the result of an overnight exposure. 
     FIG.  5 . Hybridization of the bg28 insert to restriction digests of chicken genomic DNA from birds of  B -region recombinant haplotype.  Pvu II-digested genomic DNA (5 μg each) from chickens of either the parental  B   15  and  B   21  haplotypes or the recombinant  B   15r1  and  B   21r3  haplotypes were subjected to electrophoresis on an 0.8% agarose gel and hybridized within the gel to  32 P-labeled bg28 insert. DNA samples are labeled according to their respective haplotype (see Table 1). The lane marked C contained 10 pg of  Hind III-linearized Bluescript plasmid containing the bg28 insert. On the left are molecular size markers (in kilobase pairs) based on a  Hind III digest of phage λ. The above autoradiogram was the result of an overnight exposure. 
     FIG.  6 . SEQ ID NO: 1 Partial nucleotide sequence of the bg28 insert and the corresponding amino-acid sequence, determined by the dideoxy-chain-termination method of nucleotide sequencing on one strand only of bg28 cloned cDNA. 
     FIG.  7 . SEQ ID NO: 2 Nucleotide sequence of the bg28 insert, determined by the dideoxy-chain-termination method of nucleotide sequencing of both strands of bg28 cloned cDNA. 
     FIG.  8 . Southern blot analyses of hybridization between bg32.1 and chicken genomic DNA. DNA samples are from birds of  B   15  haplotype disomic (2×), trisomic (3×) and tetrasomic (4×) for the  B  system-bearing microchromosome and from birds of  B   15r1 ,  B   21r3 , and  B   21  haplotypes. Pvu II-digested genomic DNA samples (5 μg each) were subjected to electrophoresis in an 0.8% agarose gel and hybridized within the gel to  32 P-labeled bg32.1 insert. On the left are molecular size markers (in kilobase pairs) based on a Hind III digestion of phage λ. The autoradiogram is the result of an overnight exposure. 
     FIGS. 9A and 9B. Hybridization of the bg28 (A) and bg32.1 (B) probes to restriction digests of chicken genomic DNA from birds of 17 standard haplotypes. Pvu II-digested genomic DNA (5 μg each sample) were subjected to electrophoresis in an 0.8% agarose gel and hybridized within the gel to the  32 P-labeled probes. DNA samples are labeled according to their respective  B  haplotype (see Table 3). Molecular size markers (in kilobase pairs) are based on a Hind III digestion of phage λ. The autoradiograms are the result of overnight exposures. 
     FIG.  10 . Hybridization of the bg28 probe to genomic DNA (5 μg each lane) from birds of  B   4  and  B   11  haplotypes digested with Pvu II, Bam HI, Eco RI, Hind III and Pst I. On the left are molecular size markers (in kilobase pairs) based on a Hind II digestion of phage λ. The autoradiogram is the result of an overnight exposure. 
     FIG.  11 . SEQ ID NO: 3 Nucleotide sequence of bg32.1 
     FIG.  12 . SEQ ID NO: 4 Nucleotide sequence of bg11. 
     FIG.  13 . SEQ ID NO: 5 Nucleotide sequence of bg14. 
     FIG.  14 . SEQ ID NO: 6 Nucleotide sequence of bg3. 
     FIG.  15 . SEQ ID NO: 7 Nucleotide sequence of bg8. 
     FIG.  16 . SEQ ID NO: 8 Nucleotide sequence of bg17. 
     FIG.  17 . SEQ ID NO: 9 Nucleotide sequence of gi6. 
     FIG.  18 . SEQ ID NO: 10 Nucleotide sequence of gi9. 
     FIG.  19 . SEQ ID NO: 11 Nucleotide sequence of gi11. 
     FIG.  20 . SEQ ID NO: 12 Nucleotide sequence of a 4.757 Kb fragment of chicken genomic DNA to which all the cDNA clones will hybridize under stringent conditions (in overnight. aqueous solution hybridizations at 65° C. in 5×SSPE, 5×Denhardt&#39;s, 1% SDS, 100 ug/ml salmon sperm DNA,  32 P-labeled denatured probe, followed by a 65° C. stringent washrin 0.5×SSC). 
     FIG.  21 . Percent similarity among the bg CDNA clone sequences as exemplified by comparison of all clones to bg14 using the ALIGN program in the DNASTAR. 
     FIG.  22 . SEQ ID NOS: 13-15 Comparison of the peptide sequence of two  B-G  21 peptides with the predicted amino acid sequences of bg14 and bg11 CDNA clones. 
     FIG.  23 . Hybridization of the bg11 probe to restriction digests of turkey genomic DNA from three inbred lines. Sst 1-digested DNA samples (10 ug each sample) were subjected to electrophoresis; in an 0.8% agarose gel, alkaline transferred by positive pressure displacement into a hybridization membrane (NEN Gene Screen), baked for 1 hour at 80° C., briefly UV cross-linked. Hybridization was carried out at 60° C. in aqueous solution overnight (5×SSPE, 5×Denhardt&#39;s, 1% SDS, 100 ug/ml salmon sperm DNA,  32 P-labeled denatured probe). Wash conditions were as follows: (a) a room temperature wash for 5 min. in 2×SSC (sodium chloride/sodium citrate), (b) followed by 60° C. stringent temperature wash for 30 min. in 0.5×SSC +1% SDS (sodium dodecy:L sulfate) and (c) a second room temperature wash for 5 min. in 2×SSC to remove the SDS before an overnight exposure of film to the membrane. 
     FIG.  24 . Hybridization of the bg32.1 probe to restriction digests of pheasant DNA samples (10 ug each digested with Pvu II) from a family of pheasants (dam, sire and four progeny) in which  B  haplotypes have been defined by serological methods. Conditions of hybridization and washing are identical to those provided in FIG.  22 . 
     FIG.  25 . SEQ ID NO: 16 Sequence of a complete  B-G  gene. Included is a portion of the DNA upstream from the transcription start site. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Pursuant to this invention, probes are provided by cloning of CDNA fragments from genes found within the B-G subregion of the MHC of a domesticated fowl, e.g., a chicken. With these probes, the presence of multiple alleles within the B-G subregion, a subregion of the  B  region encompassing multiple  B-G  loci, is demonstrated through homologous DNA hybridization of the  B-G  gene sequences in genomic DNA cut with a restriction enzyme, electrophoresed and analyzed in a Southern hybridization carried out either directly in the agarose matrix of the electrophoretic gel or in hybridization-membranes into which the DNA has been transferred. RFLP patterns which appear to be typical for each of a plurality of B-G alleles are described. Probes subsumed by the invention including synthetic oligonucleotide probes synthesized based on the sequences of the  B-G  CDNA clones described herein provide a new means of haplotyping chickens and other domesticated fowl including poultry (principally in the Order Galliformes) and game birds (principally in the Orders Anseriformes and Galliformes). 
     In one embodiment of the invention, a CDNA clone bg28 for a B-G antigen of the chicken major histocompatibility complex (MHC) was isolated by screening of a lambda gtll CDNA library constructed from chicken embryo erythroid cell poly((A + ) RNA. The identity of the cDNA clone as one encoding a B-G antigen was confirmed (1) by demonstrating that the clone is complementary to an erythroid cell-specific messenger RNA, (2) by obtaining the predicted patterns of hybridization of the clone with restriction endonuclease digested genomic DNA from inbred, MHC recombinant and polysomic chicken lines, and (3) by demonstrating the specific reactivity of antibodies monospecific for the fusion protein of this clone with B-G antigen protein. 
     Screening of the lambda gt11 cDNA library. A previously described lambda gt11 library,1/ the M library prepared from gradient-fractionated poly (A) +  erythroid cell RNA was screened essentially as described previously.2/ Overnight cultures of  E. coli  strain Y10883/ were infected with 50,000 plaque-forming units of recombinant lambda gt11, suspended in top agarose, and plated on 150 mm TYE-plates. Two plates were prepared for each of five aliquots of the amplified M library. The rabbit antiserum prepared against purified B-G21 was preabsorbed by the addition of 4 mg/ml ovalbumin, and by mixing 250 μl of the antiserum with Y1088 cells from a 10 ml overnight culture, spun down and resuspended in 10 ml of G buffer (TBS containing 0.1% gelatin). After 30 minutes incubation on ice, the cells were spun out and the antibody containing solution was then poured onto the surface of a 150 mm plate containing confluently lysed Y1088 cells infected with wild type lambda gt11. After an additional 30 minutes incubation on this plate (with rocking), the antibody containing solution was collected and the debris removed by centrifugation. It was then diluted to a final volume of 125 ml with GT and added to the filters. The additional steps in screening are as previously described (Moon, et al., 1985). Approximately 100 plaques were found to react positively with the rabbit anti-β-G21 serum. Thirty of these were picked for a second screening, the majority of which were again positive on the second screening. From these, six clones of varying intensity of reactivity with the antiserum were picked for further study. Three of these were subcloned. 
     1/ See Moon, et al.,  J.Cell Biol.  100:152-160 (1985).  
     2/ See Young, et al.,  Proc.Nat.Acad.Sci.  80:1194-1198 (1983).  
     3/ See Young, et al.,  Science  222:778-782 (1983).  
     Subcloning lambda qt11 inserts into M13 and Bluescript. cDNA inserts were obtained from recombinant clones of lambda gt11 by digestion with  EcoR1 . Insertion into the M13 and Bluescript (Stratagene) vectors was carried out by mixing the digested recombinant clones with the new vector in a ratio of 3:1 and religating. Recombinant colonies were selected using X-gal plates. The subclone with the longest insert 0.5 kb in size, designated bg28, was selected for further analysis. 
     Antiserum 7 used in identifying those clones was prepared against purified B-G21 antigen and was demonstrated to be specific for B-G antigens and for bg28 fusion protein in Western blot preparations. The presence of antibodies within this antiserum which recognize epitopes shared by the fusion protein product and B-G21 protein was also demonstrated. Antibodies affinity-purified with the bg28 lysogen lysate were found to bind to B-G21 antigen in immunoblots. See FIG.  1 . 
     Preparation of fusion protein B-G28.  E. coli  strain Y1089 (supF)4/ were infected with the lambda gt11 recombinant clones, colonies replica plated and lysogens selected as previously described.5/ One lysogen, grown up in an overnight culture, was inoculated into 25 ml TYE media and incubated at 32° C. to an OD 600  of 0.6. The cells were then heat shocked at 42° C. for 20 minutes, IPTG added to a final concentration of 10 mM, and incubation continued at 37° C. for two hours. Parallel cultures of the lambda gt11 wild type and an uninduced culture of the lysogen were prepared to serve as controls. The cultures were harvested by pelleting the cells, resuspending in PBS and 0.1% phenyl methyl sulfonyl fluoride (PMSF). The cells were lysed by sonication, the cellular debris removed by centrifugation, and the resulting supernatants were used as a, source of the bg28 fusion protein. 
     4 See Young, et al.,  Science  222:778-782 (1983).  
     5 See Cox et al.,  J.Cell Biol.  100:1548-1557 (1985).  
     Hybridization of bq28 cDNA insert to transcripts from erythroid and nonerythroid cells. Poly(A) +  RNA was isolated from different tissues of 14-day chick embryos. The RNA samples were subjected to denaturing agarose gel electrophoresis, capillary blotted into hybridization membranes and hybridized with  32 P-labeled bg28 cDNA insert. Only for the erythroid cells, the only cells known to carry B-G antigen, was a hybridizing MRNA species found (FIG.  2 A). The lack of hybridization seen for other tissues were not due to RNA degradation since the same samples were shown to hybridize to a β-actin probe in a parallel hybridization experiment (FIG.  2 B). Bursa poly(A) +  RNA was similarly analyzed with both probes and was found to hybridize to only the β-actin probe (data not shown). The size of the erythroid mRNA that hybridized to the bg28 insert was 2.1 kb, which is sufficiently long to encode a protein of 48 kDa. 
     Hybridization of bg28 to genomic DNA from chickens differing at the B system loci. Additional evidence for the identity of bg28 as a cDNA clone from the B-G region of the chicken MHC are provided by the patterns of hybridization of this clone to restriction endonuclease-digested genomic DNA from chickens differing in MHC haplotype, as shown in Table 1. 
     
       
         
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Sources of Blood Samples Used in Southern Analyses 
               
             
          
           
               
                   
                 B 
                   
                   
                   
                   
               
               
                   
                 Haplo- 
                 B-G 
                   
                   
                   
               
               
                   
                 type 1   
                 Allele 
                 Line 
                 FIGURE 
                 Source 
               
               
                   
                   
               
               
                   
                 B 15   
                 B-G 15   
                 diploid 
                 3 
                 Cornell a   
               
               
                   
                 B 15   
                 B-G 15   
                 trisomic 
                 3 
                 Cornell a   
               
               
                   
                 B 15   
                 B-G 15   
                 tetrasomic 
                 3 
                 Cornell a   
               
               
                   
                 B 4   
                 B-G 4   
                 CC 
                 4 
                 Basel b   
               
               
                   
                 B 12   
                 B-G 12   
                 CB 
                 4 
                 Basel b   
               
               
                   
                 B 17   
                 B-G 17   
                 UCD-003 
                 4 
                 Davis c   
               
               
                   
                 B 18   
                 B-G 18   
                 UCD-253 
                 4 
                 Davis c   
               
               
                   
                 B 19   
                 B-G 19   
                 UCD-235 
                 4 
                 Davis c   
               
               
                   
                 B 23   
                 B-G 23   
                 UNH-105 
                 4 
                 DeKalb d   
               
               
                   
                 B 24   
                 B-G 24   
                 UNH-105 
                 4 
                 DeKalb d   
               
               
                   
                 B Q   
                 B-G Q   
                 UCD-001 
                 4 
                 Davis c   
               
               
                   
                 B 15   
                 B-G 15   
                 UCD-315 
                 5 
                 Davis c   
               
               
                   
                 B 15r1   
                 B-G 21   
                 — 
                 5 
                 Basel e   
               
               
                   
                 B 21   
                 B-G 21   
                 UCD-330 
                 5 
                 Davis c   
               
               
                   
                 B 21r3   
                 B-G 15   
                 — 
                 5 
                 Basel e   
               
               
                   
                   
               
               
                   
                   1 Assignment of haplotype based on Chicken MHC Nomenclature Workshop; see Briles, et al., Immunogenetics 15: 441-447 (1982).  
               
               
                   
                   a Bloom, et al., J. Heredity 76:146-154 (1985).  
               
               
                   
                   b Hasek, et al., Folia biol. (Praha), 12: 335-341 (1966).  
               
               
                   
                   c Abplanalp, Inbred lines as genetic resources of chickens. Proceedings of the Third World Congress of Genetics Applied to Livestock Production, Lincoln, Nebraska, Vol. X, pp. 257-268 (1986).  
               
               
                   
                   d Briles, et al., Immunogenetics 15: 449-452 (1982).  
               
               
                   
                   e Koch, et al., Tissue Antigens 21: 129-137 (1983).  
               
             
          
         
       
     
     A first line of evidence supporting the designation of bg28 as a MHC clone was obtained by the analysis of genomic DNA from disomic, trisomic and tetrasomic chickens of  B   15  haplotype. The recent demonstration of a linkage between the major histocompatibility ( B ) complex and the nucleolar organizer on a microchromosome in the chicken6/ has made it possible to select polysomics of a single haplotype. As would be expected if the bg28 clone were an MHC element, an increasing intensity of hybridization was obtained between the probe genomic DNA prepared from diploid, trisomic and tetrasomic birds. See FIG. 3, three samples on left. In contrast, hybridization of an actin probe is uniform across the three samples. See FIG. 3, three samples on right. 
     In the second set of Southern hybridizations, bg28 was hybridized with PvuII-digested DNA from eight lines of chickens differing at the MHC (see FIG.  4 ), restriction fragment length polymorphisms would be predicted if the clone is indeed from this region of the chicken genome. Antigens of the chicken MHC have been demonstrated previously to be polymorphic both immunologically7/ and biochemically. A polymorphic pattern of restriction fragment lengths is evident when bg28 is used as a probe. 
     The third line of evidence from genomic DNA studies for the designation of bg28 as a chicken MHC clone, and for its identity with the B-G subregion is provided by the pattern of hybridization of this clone with DNA from MHC recombinant haplotypes. Substantially reciprocal recombinants, designated as  B   15r1  and  B   21r3  which are  B-G   21 - B-F   15  and  B-G   15 - B-F   21 , respectively, provide a means of further testing the bg28 clone for assignment to the B-G subregion. As would be predicted, the restriction fragment length pattern of hybridization of this probe with both recombinants produces a pattern indicating that the B-G subregion is that which has been cloned. See FIG.  5 . 
     6/ See Bloom, et al.,  J. Heredity  76:146-154 (1985).  
     7/ See Briles, et al.,  Immunogenetics  15:441-447 (1982).  
     Sequence of the bg28 and comparison of the amino acid composition translated sequence with the amino acid composition of purified protein. bg28 was subcloned into M13mp19 and the entire insert sequenced in one direction by the dideoxy-chain-termination method. Translation of this nucleotide sequence and its complement into peptide sequence in all six reading frames produced only one peptide without internal stop codons. See FIGS. 6 and 7. Two nucleotide sequences of bg28 are presented. The first determination was made by sequencing only one strand of the cloned fragment, and the second was a full sequence determination on both strands;. The two sequences determinations are 99% identical. The differences between the first and second determinations are minor, they consist of: (1) a change from G&gt;C at position 72, (2) the deletion of ATC at positions 258-260, (3) the deletion of A at position 354, (4) the insertion of A at position 490, and (5) the transposition of GC to CG at positions 506-507. The differences are of such a minor nature that probes of either sequence would provide identical RFLP patterns in Southern hybridizations. As Table 2 shows, the amino acid composition of this peptide (genotype unknown) compares well with the amino acid composition of the B-G21. 
     
       
         
               
             
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Amino Acid Composition Comparison 
               
             
          
           
               
                   
                 B-G21 
                 Translated 
                   
               
               
                   
                 antigen 
                 bg28 
                 Ratio 
               
               
                   
                   
               
             
          
           
               
                   
                 Ala 
                  41 
                  11 
                 3.7 
               
               
                   
                 Cys 
                  6 
                  5 
                 1.2 
               
               
                   
                 Phe 
                  37 
                  13 
                  2.85 
               
               
                   
                 His 
                  12 
                  4 
                 3   
               
               
                   
                 Ile 
                  17 
                  10 
                 1.7 
               
               
                   
                 Lys 
                  48 
                  8 
                 4.2 
               
               
                   
                 Leu 
                  48 
                  15 
                 3.2 
               
               
                   
                 Met 
                  8 
                  2 
                 4   
               
               
                   
                 Asx 
                  39 
                  14 
                 2.8 
               
               
                   
                 (Asn or Asp) 
                   
                   
                   
               
               
                   
                 Pro 
                  17 
                  1 
                 17   
               
               
                   
                 Glx 
                  70 
                  21 
                 3.3 
               
               
                   
                 (Gln or Glu) 
                   
                   
                   
               
               
                   
                 Arg 
                  31 
                  18 
                 1.7 
               
               
                   
                 Ser 
                  24 
                  17 
                 2.1 
               
               
                   
                 Thr 
                  19 
                  7 
                 2.7 
               
               
                   
                 Val 
                  30 
                  17 
                 1.8 
               
               
                   
                 Trp 
                 — 
                  3 
                 — 
               
               
                   
                 Tyr 
                  13 
                  5 
                 2.6 
               
               
                   
                 TOTAL 
                 431 
                 167 
                 2.6 
               
               
                   
                   
               
             
          
         
       
     
     A second cDNA probe useful in this invention and identified as bg32.1 was also subcloned into Blue-script and purified from the vector prior to labeling by random priming. 
     The bg32.1 is a 650 bp cDNA clone isolated from a lambda gt11 expression library made erythroid from erythrocyte mRNA8/ by cross-hybridization with bg32, a clone originally obtained screening the same library with antibodies prepared against purified B-G 21 antigen as described above. Under conditions of high stringency, the bg32 and bg32.1 fragments fail to hybridize with the previously described bg28 clone. However, as demonstrated previously with bg28, the bg32.1 clone can be assigned to  B  system-bearing microchromosome and further assigned to the  B-G  subregion on the basis of the patterns of hybridization with DNA from birds polysomic for the  B  system bearing microchromosome and with DNA from MHC. recombinant haplotypes (FIG.  8 ). The intensity of hybridization of the bg32.1 probe to the DNA of polysomic birds increases proportionate to the copy number of the  B  system bearing microchromosome. The bg32.1 probe can be further assigned to the  B-G  subregion on the basis of the pattern of hybridization with DNA from  B  system recombinants derived from two independent recombinant events which produced essentially reciprocal rearrangements of the B-F/B-L and B-G subregions in  B   15  and  B   21  haplotypes. The pattern of hybridization with DNA of the recombinants matches that of the  B-G  subregion contributing parental haplotype (FIG.  8 ). The nucleotide sequence of λbg32.1 is shown by FIG.  11 . 
     8/ Moon, R. T., et al.,  J. Cell Biol.  100:152-160 (1985).  
     High molecular weight DNA was isolated from blood samples collected from birds of known  B  system haplotype carried in several different flocks (see Table 3). 
     
       
         
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 B-G Genotypes Analyzed 
               
             
          
           
               
                 B-G 
                 B 
                   
                   
                 FIG.(S) 
                 Sample 
                   
               
               
                 Allele 
                 Haplo-Type 
                 Line 
                 Status 
                 Illustrating 
                 Size 
                 Source 
               
               
                   
               
               
                 B-G 2   
                 B 2   
                 RPRL-15.7-2* 
                   C+ 
                 2 
                 3 
                 East Lansing# 
               
               
                 B-G 2   
                 B 2   
                 RPRL-15.6-2 
                 I,C 
                 — 
                 3 
                 East Lansing 
               
               
                 B-G 2   
                 B 2   
                 UCD-331 
                 I,C 
                 — 
                 3 
                 Davis 
               
               
                 B-G 2   
                 B 2   
                 Reference Stock 
                 S 
                 — 
                 1 
                 DeKalb 
               
               
                 B-G 3   
                 B 3   
                 UCD-313 
                 I,C 
                 2 
                 2 
                 Davis 
               
               
                 B-G 4   
                 B 4   
                 PR-CC* 
                 I,C 
                 2,3 
                 1 
                 Basel 
               
               
                 B-G 5   
                 B 5   
                 RPRL-15.151-5* 
                 I 
                 2 
                 2 
                 East Lansing 
               
               
                 B-G 6   
                 B 6   
                 G-B 2*   
                 I 
                 2 
                 1 
                 Athens 
               
               
                 B-G 10   
                 B 10   
                 Reference Stock* 
                 S 
                 2 
                 2 
                 DeKalb 
               
               
                 B-G 11   
                 B 11   
                 Wis 3* 
                 S 
                 2,3 
                 2 
                 DeKalb 
               
               
                 B-G 12   
                 B 12   
                 PR-CB *   
                 I,C 
                 2 
                 1 
                 Basel 
               
               
                 B-G 12   
                 B 12   
                 RPRL 15.C-12 
                 I,C 
                 — 
                 2 
                 East Lansing 
               
               
                 B-G 13   
                 B 13   
                 G-B1* 
                 I 
                 2 
                 1 
                 Athens 
               
               
                 B-G 13   
                 B 13   
                 RPRL 15.p-13 
                 I,C 
                 — 
                 2 
                 East Lansing 
               
               
                 B-G 14   
                 B 14   
                 UCD-316 
                 I,C 
                 2 
                 2 
                 Davis 
               
               
                 B-G 15   
                 B 15   
                 RPRL-151 5 -15* 
                 I,C 
                 2 
                 2 
                 East Lansing 
               
               
                 B-G 15   
                 B 15   
                 Polysomic 
                 S 
                 1 
                 9 
                 Ithaca 
               
               
                 B-G 15   
                 B 15   
                 UCD-254 
                 I,C 
                 4 
                 2 
                 Davis 
               
               
                 B-G 15   
                 B 15   
                 UCD-011 
                 I 
                 — 
                 2 
                 Davis 
               
               
                 B-G 15   
                 B 15   
                 UCD-057 
                 I 
                 — 
                 2 
                 Davis 
               
               
                 B-G 15   
                 B 15   
                 UCD-035 
                 I 
                 — 
                 1 
                 Davis 
               
               
                 B-G 15   
                 B 21r3 , 
                 R 5′ , UCD-386 
                 I,R 
                 — 
                 2 
                 Basel/Davis 
               
               
                 B-G 15   
                 B 15   
                 UCD-396(BN) 
                 I 
                 — 
                 1 
                 Davis 
               
               
                 B-G 17   
                 B 17   
                 UCD-003* 
                 I,C 
                 2,4 
                 4 
                 Davis 
               
               
                 B-G 18   
                 B 18   
                 UCD-253* 
                 I,C 
                 2 
                 2 
                 Davis 
               
               
                 B-G 19   
                 B 19   
                 RPRL.15.P-19* 
                 I,C 
                 2 
                 2 
                 East Lansing 
               
               
                 B-G 19   
                 B 19   
                 UCD-335 
                 I,C 
                 2 
                 2 
                 Davis 
               
               
                 B-G 21   
                 B 21   
                 RPRL.15N-21* 
                 I,C 
                 2 
                 3 
                 East Lansing 
               
               
                 B-G 21   
                 B 21   
                 UCD-330 
                 I,C 
                 1 
                 &gt;20    
                 Davis 
               
               
                 B-G 21   
                 B 21   
                 UCD-100 
                 I 
                 — 
                 5 
                 Davis 
               
               
                   
                   
                 (Australorp) 
               
               
                 B-G 21   
                 B 21   
                 Ref. Stock 
                 S 
                 — 
                 1 
                 DeKalb 
               
               
                 B-G 21   
                 B 15r1   
                 R 4 , UCD-387 
                 I,R 
                 1 
                 2 
                 Basel/Davis 
               
               
                 B-G 23   
                 B 23   
                 UNH-105* 
                 S 
                 2 
                 1 
                 DeKalb 
               
               
                 B-G 24   
                 B 24   
                 UNH-105* 
                 S 
                 2 
                 1 
                 DeKalb 
               
               
                 B-G 24   
                 B 24   
                 UCD-312 
                 I 
                 — 
                 1 
                 Davis 
               
               
                 B-G C   
                 B C   
                 UCD-342 
                 I,C 
                 — 
                 1 
                 Davis 
               
               
                   
                   
                 (Ceylonese X 
               
               
                   
                   
                 Red Jungle Fowl) 
               
               
                 B-G J   
                 B J   
                 UCD-333 
                 I 
                 — 
                 1 
                 Davis 
               
               
                   
                   
                 (Red Jungle Fowl) 
               
               
                 B-G O   
                 B O   
                 UCD-104 
                 I,C 
                 — 
                 1 
                 Davis 
               
               
                 B-G Q   
                 B Q   
                 UCD-336 
                 I 
                 — 
                 1 
                 Davis 
               
               
                   
                   
                 (Red Jungle Fowl) 
               
               
                   
               
               
                 *Reference lines used as the type population in standardizing the B system nomenclature (see Briles et al., Immunogenetics 15:441-447 (1982)), although the RPRL samples are now represented by congenic lines.  
               
             
          
         
       
     
     Samples were taken from one or more individuals of each flock examined. FIGS. 9A and 9B depict patterns of hybridization between bg28 and bg32.1 and Pvu II digested DNA from a single representative from each of the 17 standard haplotypes examined. Multiple DNA restriction fragments, 4-10 per haplotype ranging size from approximately 1 to about 10 Kb are detected by the two probes. Some fragments are common to the patterns produced by both probes. For example, the three largest fragments in the  B-G   21  patterns produced with both probes appear identical. Other fragments are detected only by one or the other of the probes. A number of the restriction fragments appear to be widely shared among the haplotypes, although with the exception of perhaps one fragment of about 5.2 Kb present in Pvu II-digested DNA probed with bg28, none are shared in common across all the haplotypes examined. The  B-G  subregions are each so different, as reflected in the restriction fragment patterns, that generally the different genotypes can be distinguished readily from each other in a Southern hybridization using this single restriction enzyme and either of the two  B-G  c-DNA probes. The only exceptions appear to be the patterns produced by DNA from birds of  B   4  and  B   11  haplotypes. The other important finding is that without exception the restriction fragment patterns were the same for each  B-G  allele across the samples included in this study including samples obtained from different populations known on the basis of serological typing to carry the same  B  haplotypes. 
     In order to distinguish clearly the B-G genotype of  B   4  and  B   11  birds, it was necessary to employ additional restriction enzymes. Among the digestions with five restriction enzymes only those produced with Eco RI provided patterns clearly differentiating the two  B-G  genotypes (FIG.  10 ). It is notable that even with this enzyme the patterns of the two haplotypes differ only by a proportionate shift in the size of two restriction fragments out of the seven fragments produced. 
     Additional cDNA probes derived from erythrocytic mRNA of  B   21  haplotype useful in this invention and identified as bg11 (FIG.  12 ), bg14 (FIG.  13 ), bg3 (FIG.  14 ), bg8 (FIG. 15) and bg17 (FIG.  16 ), as well as the additional clones gi6 (FIG.  17 ), gi9 (FIG. 18) and gi11 (FIG. 19) derived from mRNA of the small intestine (also  B   21 ) were also subcloned into Bluescript, fully sequenced and found to have properties like those of bg28 and bg32.1 when employed in the Southern hybridizations. The strong sequence similarity among all the cDNA clones is depicted in FIG. 20 where all the cDNA clone sequences are compared to bg14 (a full length cDNA clone having no intronic sequences) using the ALIGN program in DNASTAR. (ALIGN is an algorithm for optimal local alignment of two partially homologous DNA sequences.) These sequences, encompassing full-length (also including introns in some), near the full-length or partial lengths of transcripts for individual  B-G  polypeptides, all show significant sequence similarity with bg14. Moreover, bg14 shows significant similarity to the nucleotide sequence of a 4.757 Kb fragment of chicken genomic DNA, typifying a segment of genomic DNA to which these B-G cDNA clones would hybridize will hybridize under straight conditions. Using the SEQCOMP program in DNASTAR (an algorithm appropriate for alignment with very large sequences in a reasonable length of time by time locating regions of perfect match and then optimizing fit) sequences the similarity between the two sequences is 89%. 
     Analysis of these sequences have provided an understanding of the organization of the  B-G  transcripts and prediction of the amino acid sequence of the  B-G  polypeptides. For purposes of illustration the organization of bg14 is described. The fully processed transcript cloned in bg14 is 1816 bp. It contains both 5′- and 3′-noncoding sequences. An open reading frame corresponds to a 398 amino acid polypeptide (including signal peptide) with calculated  M   r  45,298. Within the coding region there are sequences for: (a) a N-terminal signal peptide of 34 amino acids, (b) a single extracellular domain (amino acid residues 35-148), (c) a transmembrane domain (residues 149-178), and (d) a cytoplasmic region made up from a series of domains (residues 179-398). The single extracellular domain has properties that identify as highly similar to members of the immunoglobulin gene superfamily. The intracellular domains are characterized by a strong heptad pattern, repeats of seven amino acids the seventh residue of which is nearly always hydrophobic. This pattern is consistent with the primary sequence patterns of molecules β-alpha helical coiled coil conformation. All the cDNA clones are similarly organized. Some are missing portions of the full transcript sequence (for example bg17 is missing a portion of the 5′ end and bg11 is missing a small portion at the 3′ end) and some contain unprocessed introns (bg8, for example, possesses 9 unprocessed introns; bg11 contains 1). Comparisons of the sequences bg28 and bg32.1 with the sequences of clones full transcripts provide evidence that these probes encompass respectively portions of the 5′ end and 3′ end of  B-G  transcripts. 
     Since none of the transcripts represented in the sequences of these clones are identical, except for bg14 and bg8 which apparently represent the same transcript type and differ only by the presence of intronic sequences with bg8 and a single, silent base difference, there is now evidence for the expression of 8 transcript types. Six of these are from libraries of  B   21  haplotype and the remaining two, bg28 and bg32.1 are from birds of unknown genetic background. Hence the multiple transcript types provide evidence for the expression of alleles are multiple loci within the  B-G  subregion. Probes derived from these cDNA clones hybridize under stringent conditions (e.g., overnight aqueous hybridization in 5×SSPE, 5×Denhardt&#39;s, 1% SDS, 100 ug/ml salmon sperm DNA,  32 P-labeled denatured probe at 65° C. and stringent temperature wash at 65° C. in 0.5×SSC) to multiple bands in Southern hybridizations with genomic DNA from chickens of many different haplotypes, as illustrated by FIGS. 3,  4 ,  5 ,  9  (A and B), and  10 . Hybridization temperatures and wash temperatures of from about 55° C. to about 70° C. are appropriate. 
     These sequences and subsequences derived from them for the production of synthetic oligonucleotide probes have the capability for producing RFLP patterns by hybridization with gene sequences in other bird species. Illustrated in FIG. 23 is the hybridization of bg11 under moderately high stringency (overnight aqueous hybridization in 5×SSPE, 5×Denhardt&#39;s, 1% SDS, 100 ug/ml salmon sperm DNA,  32 P-labeled denatured probe at 60° C. and stringent temperature wash at 60° C. in 0.5×SSC) and produces polymorphic band patterns with Sst 1 digested genomic from turkeys. 
     The capability of these probes to produce RFLP patterns in genomic DNA of other bird species is further illustrated by FIG. 24 where bg32.1 hybridizes to multiple, polymorphic bands in genomic DNA from a family of ring-necked pheasants serologically  B  typed. 
     
       
         
           
             16 
           
           
             
               525 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             
               CDS 
                1..525 
             
             1
GAC ATC AGA TGG ATC CAG CAG CGG TCC TCT CGG CTT GTG CAC CAC TAC       48
Asp Ile Arg Trp Ile Gln Gln Arg Ser Ser Arg Leu Val His His Tyr
  1               5                  10                  15
CGA AAT GGA GTG GAC CTG GGG CAC ATG GAG GAA TAT AAA GGG AGA ACA       96
Arg Asn Gly Val Asp Leu Gly His Met Glu Glu Tyr Lys Gly Arg Thr
             20                  25                  30
GAA CTG CTC AGG GAT GGT CTC TCT GAT GGA AAC CTG GAT TTG CGC ATC      144
Glu Leu Leu Arg Asp Gly Leu Ser Asp Gly Asn Leu Asp Leu Arg Ile
         35                  40                  45
ACT GCT GTG ACC TCC TCT GAT AGT GGC TCC TAC AGC TGT GCT GTG CAA      192
Thr Ala Val Thr Ser Ser Asp Ser Gly Ser Tyr Ser Cys Ala Val Gln
     50                  55                  60
GAT GGT GAT GCC TAT GCA GAA GCT GTG GTG AAC CTG GAG GTG TCA GAC      240
Asp Gly Asp Ala Tyr Ala Glu Ala Val Val Asn Leu Glu Val Ser Asp
 65                  70                  75                  80
CCC TTT TCT ATG ATC ATC ATC CTT TAC TGG ACA GTG GCT CTG GCT GTG      288
Pro Phe Ser Met Ile Ile Ile Leu Tyr Trp Thr Val Ala Leu Ala Val
                 85                  90                  95
ATC ATC ACA CTT CTG GTT GGG TCA TTT GTC GTC AAT GTT TTT CTC CAT      336
Ile Ile Thr Leu Leu Val Gly Ser Phe Val Val Asn Val Phe Leu His
            100                 105                 110
AGA AAG AAA GTG GCA CAA GAG CAG AGA GCT GAA GAG AAA AGA TGC AGA      384
Arg Lys Lys Val Ala Gln Glu Gln Arg Ala Glu Glu Lys Arg Cys Arg
        115                 120                 125
GTT GGT GGA GAA AGC TGC AGC ATT GGA GAG AAA AGA TGC AGA GTT GGC      432
Val Gly Gly Glu Ser Cys Ser Ile Gly Glu Lys Arg Cys Arg Val Gly
    130                 135                 140
GGA ACA AGC AGC GCA ATC GAA GCA AAG AGA TGC AAT GTT GGA CAA ACA      480
Gly Thr Ser Ser Ala Ile Glu Ala Lys Arg Cys Asn Val Gly Gln Thr
145                 150                 155                 160
CGT TCT AAA CTG GAG GAA AGA CAG AGC AAG TGG AGA TTG GAA TTC          525
Arg Ser Lys Leu Glu Glu Arg Gln Ser Lys Trp Arg Leu Glu Phe
                165                 170                 175 
           
           
             
               523 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             2
GACATCAGAT GGATCCAGCA GCGGTCCTCT CGGCTTGTGC ACCACTACCG AAATGGAGTG     60
GACCTGGGGC AGATGGAGGA ATATAAAGGG AGAACAGAAC TGCTCAGGGA TGGTCTCTCT    120
GATGGAAACC TGGATTTGCG CATCACTGCT GTGACCTCCT CTGATAGTGG CTCCTACAGC    180
TGTGCTGTGC AAGATGGTGA TGCCTATGCA GAAGCTGTGG TGAACCTGGA GGTGTCAGAC    240
CCCTTTTCTA TGATCATCCT TTACTGGACA GTGGCTCTGG CTGTGATCAT CACACTTCTG    300
GTTGGGTCAT TTGTCGTCAA TGTTTTTCTC CATAGAAAGA AAGTGGCACA GAGCAGAGAG    360
CTGAAGAGAA AAGATGCAGA GTTGGTGGAG AAAGCTGCAG CATTGGAGAG AAAAGATGCA    420
GAGTTGGCGG AACAAGCAGC GCAATCGAAG CAAAGAGATG CAATGTTGGA CAAACACGTT    480
CTAAAACTGG AGGAAAAGAC AGACGAAGTG GAGATTGGAA TTC                      523 
           
           
             
               634 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             3
CGGTGAACAG ATGGAGAGAA GGAATGCAAA GTTGGAGGCA GCAGCTGTAA AACTGGGACA     60
CAAAGCTAAA GAATCAGAGA AACAGAAATC GGAGCTGAAG GAGCGCCATG AGGAGATGGC    120
AGAACAAACT GAAGCAGTGG TGGTAGAAAC TGAAGAATAG GAAAAACCAT CTGAAGAATC    180
AGATTGAGAG ATGAACTGCG CCTCACAATA AGCACAGGAG TTAAGCTTCT TAGATCAATA    240
ACTGCACAGC ATACAAAACC ACAATAACTC AAACAGAGTA AGGAGGAGCC AGTGTTTGTG    300
TTGAGTGAGA ACACTGCAGT TCTGTCAGCC AAAGCTGCCT GAGGGACCGC CCAATTGAGG    360
GTGTGTGACC TCCAACTCAA ATCCAGTTGG AAGAAAGAAA CCATAGAAAG GAAGGAAAGG    420
GGAGGAAGAC AGAGATCCTG GAAGAGATAT GGGCATTTGG GGAAATAGTG TGATCATGTA    480
TCAGGCTTTG TGGACATCTA ATGAATATGT CATGCTTTTG TAACTACAAG CATGCACGCA    540
GAAACAAAGG TAGAAAACTG CTTTGGGTGT TAGCACTGTT CTCTGTCACT ATATAATAAA    600
GAATACCTGC TGATGGCAAT GGAACAAAAA AAAA                                634 
           
           
             
               1785 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             4
ATCCGTTCGA GCTCTCTCCT CCTACAGCTG CTGCCCTCAT ATTCTCCCCA CACTTCTTCC     60
CCATATTCTT TCCAAATCCT CTTCCCCATC TCCTCCACCG TCTCTTTCTC AGAGTCCTTC    120
CTCTCTCTCC CTAAATTCTT CCCCCCTCCT CTCCTCCAGC ACAGATGCGC TTCACATCGG    180
GATGCAACCA CCCCAGTTTC ACCCTCCCCT GGAGGACCCT CCTGCCTTAT CTCGTGGCTC    240
TGCACCTCCT CCAGCCGGGA TCAGCCCAGC TCAGGGTGGT GGCGCCGAGC CTCCGTGTCA    300
CTGCCATCGT GGGACAGGAT GTCGTGCTGC GCTGCCACTT GTGCCCTTGC AAGGATGCTT    360
GGAGATTGGA CATCAGATGG ATCCTGCAGC GGTCCTCTGG TTTTGTGCAC CACTATCAAA    420
ATGGAGTGGA CCTTGGGCAG ATGGAGGGAT ATAAAGGGAG AACAGAACTG CTCAGGGATG    480
GTCTCTATGA TGGAAACCTG GATTTGCGCA TCACTGCTGT GAGCACCTCC GATAGTGGCT    540
CATACAGCTG TGCTGTGCAG GATGGTGATG GCTATGCAGA CGCTGTGGTG GACCTGGAGG    600
TGTCAGATCC CTTTTCCCAG ATCGTCCATC CCTGGAAGGT GGCTCTGGCT GTGGTCGTCA    660
CAATTCTCGT TGGGTCATTT GTCATCAATG TTTTTCTCTG TAGGAAGAAA GCGGCACAGA    720
GCAGAGAGCT GAGTGAGTCC TTCCAGCCCC TTCCACCACC AAAGTCCCTT TAATGGAACT    780
GATAGAAGAC TGCAGAGTGC TGGGTTTATG CCTTGTGCTG GGGCCATGGG ATCTATGGGA    840
CCTTGGGATG TGTTGGGGCC GTGGGATGTG CTGGGGTCGT GGGATCTGTC AACCCTGATT    900
GATCCACTTC AGAACTCTTG CCCAATCGGT TCCTTCCGAT TCATTTAACT CCTTCTTGAG    960
GCCAAAGTGG TCATTGGCCA CATCCCATAA AAAAGGGTTT GGGGTCAGGG TGTGGGAGCT   1020
GATCGCATGG AAACGTGTCC CCTCTGACCA TGCATTTCAT TTGCTTCTAT TTTGCAGAGA   1080
GAAAAGATGC AGCGTTGGCG GAACTAGATG AGATATCGGG TTTAAGTGCT GAAAATCTGA   1140
AGCAATTAGC TTCAAAACTG AACGAAAATG CTGACGAAGT GGAGGATTGC AATTCAGAGC   1200
TGAAGAAAGA CTGTGAAGAG ATGGGTTCTG GCGTTGGAGA TCTGAAGGAA CTGGCTGCAA   1260
AATTGGAGGA ATATATTGCA GTGAATCGGA GAAGGAATGT AAAGTTGAAT AATATAGCTG   1320
CCAAACTGGC ACAACAAACT AAAGAATTGG AGAAACAGCA TTCACAGTTC CACAGACACT   1380
TTCAGCGTAT GGATTTAAGT GCTGTAAACC AGAAGAAACT GGTTACAAAA CTGGAGGAAC   1440
ACTTTGAATG GATGGAGAGA AGGAATGTAA AGTTGGAGAT ACCAGCTGTA ATACTGGGGC   1500
AACAAGCTAA AGAATCAGAG AAACAGAAAT CGGAGCTGAA GGAGCGCCAT GAGGAGATGG   1560
CAGAACAAAC TGAAGCAGTG GTGGTAGATA CTGAAGAAGC GGAAAAACCA TCTGAAGAAT   1620
TGGATTGAGA GATGAACTGC GCCTCACAGT AACCACAGGA GTTAAGCTTC ATAGATCAAT   1680
GACTGCACAG CATACAAAAA CCACGATACC TCAAACAGAG CAAGGAAATC CACAGCGAGA   1740
ACAAGAGGAG CCAGTGTTTG TGTTGAGTGA GAACACTGCA GTTCT                   1785 
           
           
             
               1816 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             5
TTCTGCCCTC ATATTCTCCC CACACTTCTT CCCCATATTC TTTCCAAATC CTCTTCCCCA     60
TCTCCTCCAT CGTCTCCTTC TCAGAGTCCT TCCTCTCTCT CCCTAAATTC TTCCCCCCTC    120
CTCTTCTCCA GCACAGATGG CCTTCACATC GGGCTGCAAC CACCCCAGTT TCACCCTCCC    180
CTGGAGGACC CTCCTGCCTT ATCTCGTGGC TCTGCACCTC CTCCAGCCGG GATCAGCCCA    240
GATCACGGTG GTGGCACCGA GCCTCCGTGT CACTGCCATC GTGGGACAGG ATGTTGTGCT    300
GCGCTGCCAC TTGTCCCCAT GCAAGGATGT TCGGAATTCA GACATCAGAT GGATCCAGCA    360
GCGGTCCTCT CGGCTTGTGC ACCACTACCG AAATGGAGTG GACCTGGGGC AGATGGAGGA    420
ATATAAAGGG AGAACAGAAC TGCTCAGGGA TGGTCTCTCT GATGGAAACC TGGATTTGCG    480
CATCACTGCT GTGACCTCCT CTGATAGTGG CTCCTACAGC TGTGCTGTGC AAGATGGTGA    540
TGCCTATGCA GAAGCTGTGG TGAACCTGGA GGTGTCAGAC CCCTTTTCTA TGATCATCCT    600
TTACTGGACA GTGGCTCTGG CTGTGATCAT CACACTTCTG GTTGGGTCAT TTGTCGTCAA    660
TGTTTTTCTC CATAGAAAGA AAGTGGCACA GAGCAGAGAG CTGAAGAGAA AAGATGCAGA    720
GTTGGTGGAG AAAGCTGCAG CATTGGAGAG AAAAGATGCA GAGTTGGCGG AACAAGCAGC    780
GCAATCGAAG CAAAGAGATG CAATGTTGGA CAAACACGTT CTAAAACTGG AGGAAAAGAC    840
AGACGAAGTG GAGAACTGGA ATTCAGTGCT GAAAAAAGAC AGTGAAGAGA TGGGTTATGG    900
CTTTGGAGAT CTGAAGAAAC TGGCTGCAGA ACTGGAGAAA CACTCTGAAG AGATGGGGAC    960
AAGGGATTTA AAGTTGGAGC GACTAGCTGC CAAACTGGAA CATCAAACTA AAGAATTGGA   1020
GAAACAGCAT TCACAGTTCC AGAGACACTT TCAGAATATG TATTTAAGTG CTGGAAAACA   1080
GAAGAAAATG GTTACAAAAC TGGAGGAACA CTGTGAATGG ATGGTGAGAA GGAATGTAAA   1140
GTTGGAGATA CCAGCTGTAA AAGTGGGGCA ACAAGCTAAA GAATCAGAGG AACAGAAATC   1200
GGAGCTGAAG GAGCACCATG AGGAGACGGG GCAACAAGCT AAAGAATCAG AGAAACAGAA   1260
ATCGGAGCTG AAGGAGCGCC ATGAGGAGAT GGCAGAACAA ACTGAAGCAG TGGTGGTAGA   1320
AACTGAAGAA TAGGAAAAAC CATCTGAAGA ATTGGATTGA GAGATGAACT GCGCCTCGCA   1380
GTAACCACAG GAGTTAAGCT TCATAGATCA ATAACTGCAC AGCATACAAA ACCACAATAA   1440
CTCAAACAGG GTAAGGAGGA GCCAGTGTTT GTGTTGAGTG AGAACACTGC AGTTCTGTCA   1500
GCCAAAGCTG CCTGAGGGAC CGCCCAATTG AGGGTGTGCG ACCTCCAACT CAAAGCCAAT   1560
TGGAAGAAAG AAACCATAGA AAGGAAGAAA AGGGGAGGAA GACAGAGATC CTGGAAGAGA   1620
TATGGGCATT TGGGGAAATA GTGTGACCAT GTATCAGGCT TTGTGGACAT CTAACGAATA   1680
TGTCATGTTT TTGTAAATAC AAGCATGCAC GCAGAAACAA AGGGAGAAAA CTGCTTTGGG   1740
TGTTAGCACT GTTCTCTGTC CCTATATAAT AAAGAATACC TGCTGATGGC AAAAAAAAAA   1800
AAAAAAAAAA AAAAAA                                                   1816 
           
           
             
               1822 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             6
AAATGAAGAC TTCAGGATCC TTCCATAAAA GCTATCAGTT TGACTTCAGA GAGGGCTATT     60
CTCGGTGTTT GCAAGAAGCT TTCCATCGTC TCCTTCTCAG AGTCCTTCCT CTCTCTCCCT    120
AAATTCTTCC CCCCTCCTCT TCTCCAGCAC AGATGGCCTT CACATCGGGC TGCAACCACC    180
CCAGTTTCAC CCTCCCCTGG AGGACCCTCC TGCCTTATCT CGTGGCTCTG CACCTCCTCC    240
AGCCGGGATC AGCCCAGATC ACGGTGGTGG CACCGAGCCT CCGTGTCACT GCCATCGTGG    300
GACAGGATGT TGTGCTGCGC TGCCACTTGT CCCCATGCAA GGATGTTCGG AATTCAGACA    360
TCAGATGGAT CCAGCAGCGG TCCTCTCGGC TTGTGCACCA CTACCGAAAT GGAGTGGACC    420
TGGGGCAGAT GGAGGAATAT AAAGGGAGAA CAGAACTGCT CAGGGATGGT CTCTCTGATG    480
GAAACCTGGA TTTGCGCATC ACTGCTGTGA CCTCCTCTGA TAGTGGCTCC TACAGCTGTG    540
CTGTGCAAGA TGGTGATGCC TATGCAGAAG CTGTGGTGAA CCTGGAGGTG TCAGACCCCT    600
TTTCTATGAT CATCCTTTAC TGGACAGTGG CTCTGGCTGT GATCATCACA CTTCTGGTTG    660
GGTCATTTGT CGTCAATGTT TTTCTCCATA GAAAGAAAGT GGCACAGAGC AGAGAGCTGA    720
AGAGAAAAGA TGCAGAGTTG GTGGAGAAAG CTGCAGCATT GGAGAGAAAA GATGCAGAGT    780
TGGCGGAACA AGCAGCGCAA TCGAAGCAAA GAGATGCAAT GTTGGACAAA CACGTTCTAA    840
AACTGGAGGA AAAGACAGAC GAAGTGGAGA ATTGGAATTC AGTGCTGAAA AAAGACAGTG    900
AAGAGATGGG TTATGGCTTT GGAGATCTGA AGAAACTGGC TGCAGAACTG GAGAAACACT    960
CTGAAGAGAT GGGGACAAGG GATTTAAAGT TGGAGCGACT AGCTGCCAAA CTGGAACATC   1020
AAACTAAAGA ATTGGAGAAA CAGCATTCAC AGTTCCAGAG ACACTTTCAG AATATGTATT   1080
TAAGTGCTGG AAAACAGAAG AAAATGGTTA CAAAACTGGA GGAACACTGT GAATGGATGG   1140
TGAGAAGGAA TGTAAAGTTG GAGATACCAG CTGTAAAAGT GGGGCAACAA GCTAAAGAAT   1200
CAGAGGAACA GAAATCGGAG CTGAAGGAGC ACCATGAGGA GACGGGGCAA CAAGCTAAAG   1260
AATCAGAGAA ACAGAAATCG GAGCTGAAGG AGCGCCATGA GGAGATGGAA CAAACTGAAG   1320
CAGTGGTGGT AGAAACTGAA GAATAGGAAA AACCATCTGA AGAATTGGAT TGAGAGATGA   1380
ACTGCGCCTC GCAGTAACCA CAGGAGTTAA GCTTCATAGA TCAATAACTG CACAGCATAC   1440
AAAATCACAA TAACTCAAAC AGGGTAAGGA GGAGCCAGTG TTTGTGTTGA GTGAGAACAC   1500
TGCAGTTCTG TCAGCCAAAG CTGCCTGAGG GACCGCCCAA TTGAGGGTGT GCGACCTCCA   1560
ACTCAAAGCC AATTGGAAGA AAGAAACCAT AGAAAGGAAG AAAAGGGGAG GAAGACAGAG   1620
ATCCTGGAAG AGATATGGGC ATTTGGGGAA ATAGTGTGAC CATGTATCAG GCTTTGTGGA   1680
CATCTAACGA ATATGTCATG TTTTTGTAAA TACAAGCATG CACGCAGAAA CAAAGGGAGA   1740
AAACTGCTTT GGGTGTTAGC ACTGTTCTCT GTCCCTATAT AATAAAGAAT ACCTGCTGAT   1800
GGCAATGGAA AAAAAAAAAA AA                                            1822 
           
           
             
               3134 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             7
ATCCGCTCGA GCTCTCTCCT CCTACAGTTT CTGCCCTCAT ATTCTCCCCA CACTTCTTCC     60
CCATATTCTT TCCAAATCCT CTTCCCCATC TCCTCCATCG TCTCCTTCTC AGAGTCCTTC    120
CTCTCTCTCC CTAAATTCTT CCCCCCTCCT CTTCTCCAGC ACAGATGGCC TTCACATCGG    180
GCTGCAACCA CCCCAGTTTC ACCCTCCCCT GGAGGACCCT CCTGCCTTAT CTCGTGGCTC    240
TGCACCTCCT CCAGCCGGGA TCAGCCCAGA TCACGGTGGT GGCACCGAGC CTCCGTGTCA    300
CTGCCATCGT GGGACAGGAT GTTGTGCTGC GCTGCCACTT GTCCCCATGC AAGGATGTTC    360
GGAATTCAGA CATCAGATGG ATCCAGCAGC GGTCCTCTCG GCTTGTGCAC CACTACCGAA    420
ATGGAGTGGA CCTGGGGCAG ATGGAGGAAT ATAAAGGGAG AACAGAACTG CTCAGGGATG    480
GTCTCTCTGA TGGAAACCTG GATTTGCGCA TCACTGCTGT GACCTCCTCT GATAGTGGCT    540
CCTACAGCTG TGCTGTGCAA GATGGTGATG CCTATGCAGA AGCTGTGGTG AACCTGGAGG    600
TGTCAGACCC CTTTTCTATG ATCATCCTTT ACTGGACAGT GGCTCTGGCT GTGATCATCA    660
CACTTCTGGT TGGGTCATTT GTCGTCAATG TTTTTCTCCA TAGAAAGAAA GTGGCACAGA    720
GCAGAGAGCT GAGTGAGTCC TTCCATCCCC ATCCACCAAC CAAAGTCCCT TTAATGGAAC    780
TGACAGCAGA CTGCAGAGTG CTGGGTTATG CCATGTGCTG GGGCCATGAG CTATGTTGAG    840
GCTTTGGAAT GTGTTGGGGT TGTGGGATGT ACTGGGGTCG TGGGATGTGT TATTCCTGGC    900
TGATTCACGT GGAAAAACCT TTCACAATCG GTTCCTTCCA GTTTGTTTAA TTCCTTCTTG    960
GGCCCAAAGT GGTCATTGGA CTCCTCCCAG AAAAAAGGGT TTGGGGTCAG GGTGTGAGAG   1020
CTGATGGCAC GGAAACGTGT CCCCTCTGAC CATGCATTTC ATTTGCTTCT ATTTTGCAGA   1080
GAGAAAAGAT GCAGAGTTGG GTAAGTCTCC TTCCCTAAAG CGAGGGAATT CAGGGTGTCC   1140
CCATGGCATC AGCCGTGGAA TTAGTAGCTG TCCTCTCTGA CAATTCACTG CTCTGCTCTT   1200
TCCTTTCCAG TGGAGAAAGC TGCAGCATTG GGTGAGTTAT ATTCCCCAAG CCAAAGTACT   1260
TTGGGTCTTC CCATTGGAAG TTATTTCCTC AGACCATCCT TTCTGTTGTG TTTGCTTTGG   1320
CATCATGTTA GTAAAATGCC TTCTTGGGAC CAAAGTGGTC ATTGGCCACT TCCCAGAAAA   1380
AAAGGTTTGG GGTCAGGGTG TGGGAGCTGA TGGCATGGAA ACATGTTCCC TCTGACCATG   1440
CATTTCCTTT GCTTCTTTTT CCAGAGAGAA AAGATGCAGA GTTGGCGGAA CAAGCAGCGC   1500
AATCGAGTGA GTCTCCCCCT CCATTTTTAT TATTTTTAAA TGTTCAGCCT CCGGTAGCTG   1560
TGGGATGAGA TGTTCCTCTC ATCATACACT GACTCTGCTT TTCCTTTGCA GAGCAAAGAG   1620
ATGCAATGTT GGACAAACAC GTTCTAAAAC TGGGTGAGTC CTCACTCCCA AATTATAAAG   1680
CAAAGGGTTC TGCCTGTGTG AGCTGTGGGA TCAGACGTTC CTCTCATCGT GCATTGCTTT   1740
TCTCTTTCTT TTTCAGAGGA AAAGACAGAC GAAGTGGAGA ATTGGAATTC AGTGCTGAGT   1800
AAGTTGCAGT CACTGAACTG AGGGAATGTG GGGTCTTCCT AAGGGACTGC GTAGGGGAGA   1860
AGTTCCCATG CACTGCTTTT CTCTTTCTTT TCCAGAAAAA GACAGTGAAG AGATGGGTTA   1920
TGGCTTTGGA GATCTGAGTA AGTCTCCCTC CCAACATGGA AGGAATTTAT GGTCTTAGCA   1980
TGGGATCAGC CATGGGATGA TCATCTGACC CCTCTCATCA TGCAATTCAT ATTTGTTCCT   2040
TTTGCAGAGA AACTGGCTGC AGAACTGGAG AAACACTCTG AAGAGATGGG GACAAGGGAT   2100
TTAAAGTTGG AGCGACTAGC TGCCAAACTG GAACATCAAA CTAAAGAATT GGAGAAACAG   2160
CATTCACAGT TCCAGAGACA CTTTCAGAAT ATGTATTTAA GTGCTGGAAA ACAGAGTAAG   2220
TCTCCCTCCC TGCACAGAAG GAACTTACGG TTTTCCCATG GGATCAGCCA TGGGACGATC   2280
ATCCGACTCT TCTCATCATG AATTTCGTCT TTCTTTCTTT TGCAGAGAAA ATGGTTACAA   2340
AACTGGAGGA ACACTGTGAA TGGATGGTGA GAAGGAATGT AAAGTTGGAG ATACCAGCTG   2400
TAAAAGTGGG GCAACAAGCT AAAGAATCAG AGGAACAGAA ATCGGAGCTG AAGGAGCACC   2460
ATGAGGAGAC GGGGCAACAA GCTAAAGAAT CAGAGAAACA GAAATCGGAG CTGAAGGAGC   2520
GCCATGAGGA GATGGCAGAA CAAACTGAAG CAGTGGTGGT AGAAACTGAA GAATAGGGTG   2580
AGTCTTTCCC AAACCAAAGC AATACGGGGT TTCCCATGGC ATGACAAGCT GTCCCACCTC   2640
AGCATCCGTT CCTTTTTCTT TCTTTTCCAG AAAAACCATC TGAAGAATTG GATTGAGAGA   2700
TGAACTGCGC CTCGCAGTAA CCACAGGAGT TAAGCTTCAT AGATCAATAA CTGCACAGCA   2760
TACAAAACCA CAATAACTCA AACAGGGTAA GGAGGAGCCA GTGTTTGTGT TGAGTGAGAA   2820
CACTGCAGTT CTGTCAGCCA AAGCTGCCTG AGGGACCGCC CAATTGAGGG TGTGCGACCT   2880
CCAACTCAAA GCCAATTGGA AGAAAGAAAC CATAGAAAGG AAGAAAAGGG GAGGAAGACA   2940
GAGATCCTGG AAGAGATATG GGCATTTGGG GAAATAGTGT GACCATGTAT CAGGCTTTGT   3000
GGACATCTAA CGAATATGTC ATGTTTTTGT AAATACAAGC ATGCACGCAG AAACAAAGGG   3060
AGAAAACTGC TTTGGGTGTT AGCACTGTTC TCTGTCCCTA TATAATAAAG AATACCTGCT   3120
GATGGCAAAA AAAA                                                     3134 
           
           
             
               1449 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             8
CGATGTTCGG AATTCAGACA TCAGATGGAT CCAGCTGCGG TCCTCTAGGA TTGTGCACCA     60
CTACCAAAAT GGAGAGGACC TGGATCAGAT GGAGGAATAT GAAGGGAGAA CAGAACTGCT    120
CAGGGATGGT CTCTCTGATG GAAACCTGGA TTTGCGCATC ACTGCTGTGA GCTCCTCTGA    180
CAGTGGCTCG TACAGCTGTG CTGTGCAAGA TGATGATGGC TATGCAGAAG CTGTGGTGAA    240
CCTGGAGGTG TCAGATCCCT TTTCCCAGAT CGTCCATCCC TGGAAGGTGG CTCTGCCTGT    300
GGTCGTCACA ATTCTCGTTG GGTCATTTGT CATCATTGTT TTTCTCTATA GGAAGAAAGT    360
GGCACAGAGC AGAGAGCTGA AGGGAAAAGA TGCAGCACTG GCGGAACTAC CTGCGATATT    420
GGGTGTATGT ACTGCAAATT TGAAGATCCT AGCTTCAAAA CTGATGAAAC AAATGGAAAA    480
ATTGGAGATT CAGAATTCAC TCTTGAAGAA ACGGTATGAG ATTACGGAGG AACTGGCTGC    540
AGATCTGGAG GAACATCTTG CTGAGAAGGA TTTAAGCACT GCAGATCTGA AGCTACTAGC    600
TGCAAAACTG GTGGAACAAA GAGAAGCAGT GGAGGAACGG GATTCACAGC TGAGGAAACA    660
GTATGAAAAG TTGGGTTCGC GTGCTACAAA TCTGAAGACA CAACTTAAAA AGTTGGAGAA    720
CGAAATTGAA GAAGTGGAGA AACACCTTAA AAAGATTGGT ATACGTGCTC CTAATCTGAA    780
GCTACACATG GCAGAACTGG TGGATCAAGC TGAAGCAGTG GAGAAACGGA AATCAGAGCT    840
GAAGAGCTAT TTGACAAATA TAGGTTTACG TGCTGCAGAG CTGAAAAAAT ACATTGCAGC    900
ACTGGAGAAA CGAATTGAAG CATTGGAAAC TAAAGAATTG GAACAACCAT CTAAAGAACA    960
GGATTGAAAG ATGAACTGCG CCTCACAGTA ACCACAGGAG TTAAGCTTCA TAGACTGCAG   1020
ACTGCACAGG ATAGCAACAT CGCCATAACG CAAAGCAAGC AAGGAAATCC ACACGGGGAA   1080
CAAGAGGAGC CAGTGTTTGT ATTGAGTGAG AACACTGCAG TTCTGCAAGC CACAGCTGCC   1140
TGAGGGACCA GCAAACTGAG GGTGTGTGAC CTCCATCTCA AATCCAGTTG GAAGAAAGAC   1200
ACCATAGAAA AGAAGACTAC AAGAGGAAGA CAGAGATCCT GGAAAAGGGA CAGACATTTT   1260
GGGAATGAAC ATGGCCATGT ATCAGGGTTT GAGGAATTCT AATGAATATG TAAGGCTTCT   1320
GGAAATATAA ACATGCACAC AGAAGTAAAG GTAGAAAACT GCTTTGGGTG TTAACACTGT   1380
TCTCTATCAC AATATAATAA AGAAATACCT GCTGATGGCG ATGGAAAAGA AAAAAAAAAA   1440
AAAAAAAAA                                                           1449 
           
           
             
               2217 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             
               CDS 
                252..821 
             
             
               CDS 
                1165..1647 
             
             9
GCTCCTTCTG CATATTCTTC CTGAACTTTT TCTAAATCTT CTTTCCAGAT CTTCTTCCCC     60
ATCTGCTCCA GCACCTCCTC CTTGTATCCC CTTCCCCAAT CTTCCCTTCC CCACCTCCTT    120
CTCCTATCAT CTCTCATCTT TTACCCATTT TCTACCCACC TTCTGCCCCA TCTCCTCCAT    180
CATCTCCTTC TCAGTCTCCT TCCTCTCTCT CCTTTCCCCA ACTCCTCCCC CCCTCCTCTT    240
CTCCAGCACA G ATG CAC TTC ACA TCG GGC TGC AAC CAC CCC AGT TTC ACC     290
             Met His Phe Thr Ser Gly Cys Asn His Pro Ser Phe Thr
               1               5                  10
CTC CCC TGG AGG ACC CTC CTG CCT TAT CTC ATG GCT CTG CAC CTC CTC      338
Leu Pro Trp Arg Thr Leu Leu Pro Tyr Leu Met Ala Leu His Leu Leu
     15                  20                  25
CAG CCG GGA TCA GCC CAG CAA AGG GTG GTG GCA CCG AGC CTC CGT GTC      386
Gln Pro Gly Ser Ala Gln Gln Arg Val Val Ala Pro Ser Leu Arg Val
 30                  35                  40                  45
ACT GCC ATC GTG GGA CAG GAT GTT GTG CTG CGC TGC CAG TTG TCC CCT      434
Thr Ala Ile Val Gly Gln Asp Val Val Leu Arg Cys Gln Leu Ser Pro
                 50                  55                  60
TGC AAG GAA GCT TGG AGA TCA GAC AAC AGA TGG ATC CAG CTG CGG TCC      482
Cys Lys Glu Ala Trp Arg Ser Asp Asn Arg Trp Ile Gln Leu Arg Ser
             65                  70                  75
TCT CGG CTT GTG CAC CAC TAT CAA TAT GGA TTG GAC CTG GGG CAG ATG      530
Ser Arg Leu Val His His Tyr Gln Tyr Gly Leu Asp Leu Gly Gln Met
         80                  85                  90
GAG GAA TAT AAA GGG AGG ACA GAA CTA CTC AGG AAG GGT CTC TCT GAT      578
Glu Glu Tyr Lys Gly Arg Thr Glu Leu Leu Arg Lys Gly Leu Ser Asp
     95                 100                 105
GGA AAC CTG GAT TTG CGC TTC ACT GCT GTG AGC ACC TCC GAT AAT GGC      626
Gly Asn Leu Asp Leu Arg Phe Thr Ala Val Ser Thr Ser Asp Asn Gly
110                 115                 120                 125
TCA TAC AGC TGT GCT GTG CAA GAT GAT GAT GGC TAC GGA GAC GCT GTT      674
Ser Tyr Ser Cys Ala Val Gln Asp Asp Asp Gly Tyr Gly Asp Ala Val
                130                 135                 140
GTG GAG CTG GAG GTG TCA GAT CCC TTT TCC CAG ATC GTC CAT CCC TGG      722
Val Glu Leu Glu Val Ser Asp Pro Phe Ser Gln Ile Val His Pro Trp
            145                 150                 155
AAG GTG GCT CTG GCT GTG GTT GTC ACA ATT CTG GTT GGG TCA TCT GTC      770
Lys Val Ala Leu Ala Val Val Val Thr Ile Leu Val Gly Ser Ser Val
        160                 165                 170
ATC AAT GTT TTT CTC TAT AGA AAG AAA GCT GCA CAG AGC AGA GAG CTG      818
Ile Asn Val Phe Leu Tyr Arg Lys Lys Ala Ala Gln Ser Arg Glu Leu
    175                 180                 185
AGT GAGTCCTTCC AGCACCTTCC ACCACCAAAG TCCCTTTAAT GGAACTGATA           871
Ser
190
GAAGACTGCA GAGTGCTGGG TTTATGCCAT GGGCTGGGGC TGTGGGATCT TTGGGGCTTG    931
GGATGTGTTG GGGCCGTGGG ATGTGCTGGG GTCGTGGGAT CTGTCAATCC TGATTGCTCC    991
TCTTCAGAAC TCTTGCCCAA TCGGTTCCTT CCGATTCATT TAACTCCTTC TTGGACCAAA   1051
GTGGTCATTG GCCTCTTACT AGAAAGAAAA GATTTGGGGT CTGGGTATGG GAGCAGCCAT   1111
GGGATGAGAA GGTGTTCCCT CTGACCATAC ATTTCTTTTG CTTCTATTTT GCA GAG      1167
                                                           Glu
                                                             1
AGA AAA GAT GCA ATG TTG GGT CCC GGT GCT GAA AAG CTG AAG AAA TTA     1215
Arg Lys Asp Ala Met Leu Gly Pro Gly Ala Glu Lys Leu Lys Lys Leu
              5                  10                  15
GCT TCA AAA CTG AAC GAA AAT GCT GAC GAA GTG GAG AAT TGC AAT TTA     1263
Ala Ser Lys Leu Asn Glu Asn Ala Asp Glu Val Glu Asn Cys Asn Leu
         20                  25                  30
GAG CTG AAA AAA GAC TGT GAC GAG ATG AGT TCT GCC GTT GCA GAT CTG     1311
Glu Leu Lys Lys Asp Cys Asp Glu Met Ser Ser Ala Val Ala Asp Leu
     35                  40                  45
AAG AAA TTG GCT GCA GTG ATT TGG ATA TGG GAT TTA AAG TTG TAT AAT     1359
Lys Lys Leu Ala Ala Val Ile Trp Ile Trp Asp Leu Lys Leu Tyr Asn
 50                  55                  60                  65
CTA GCT GCC AAA CTG GGA CAA CAA ACT AAA GAA CTG GAG GAA CAG CAT     1407
Leu Ala Ala Lys Leu Gly Gln Gln Thr Lys Glu Leu Glu Glu Gln His
                 70                  75                  80
TCA CAG TTC CAG GGT CAC TTT CAG CAT ATG GAT TTA AGT GCT GTA AAA     1455
Ser Gln Phe Gln Gly His Phe Gln His Met Asp Leu Ser Ala Val Lys
             85                  90                  95
CAG AAG AAA CTG GTT ACA AAA CTG GAG GAA CAC TGT AAT CAG ATG GTG     1503
Gln Lys Lys Leu Val Thr Lys Leu Glu Glu His Cys Asn Gln Met Val
        100                 105                 110
AGA AGG AAT GTA AAG TTG GAG GCA GCA GCT GTA AAA CTG GGG CAA CAA     1551
Arg Arg Asn Val Lys Leu Glu Ala Ala Ala Val Lys Leu Gly Gln Gln
    115                 120                 125
GCT AAA GAA TCA GAG GAA CAG AAA TCG GAG CTG AAG GAG CGC CAT GAG     1599
Ala Lys Glu Ser Glu Glu Gln Lys Ser Glu Leu Lys Glu Arg His Glu
130                 135                 140                 145
GAG ATG GCA GAA CAA ACT GAA GCA GTG GTG GTA GAT ACT GAA GAA TAG     1647
Glu Met Ala Glu Gln Thr Glu Ala Val Val Val Asp Thr Glu Glu  *
                150                 155                 160
GGTGAGTCTT CCCCAAACCA AAGCAATACG GGGTTTCCCA TGGCATGACA AGCTGTCCCA   1707
CCTCAGCATC CGTTGCTTTT TATTTCTTTT CCAGAAAAAC CATCTGAAGA ATTGGATTGA   1767
GAGATGAACT GCGCCTCACA GTAACCACAG GAGTTAAGCT TCATAGATCA ATTACTACAC   1827
AGCATAAAAA ACCACGATTC CACAAACAGA GCAAGGAAAT CCACAGCGAG AACAAGAGGA   1887
GCCAGTGTTT GTGTTGAGTG AGAACACTGC AGTTCTGTGA GCCAAAGCTG CCTGAGGGAC   1947
CGCCGAACTG AGGGTGTGCG ACCTCCAACT CAAAGCAATT GGAAGAAAGA AACCATAGAA   2007
AGGAAGGAAA GGGGAGGAAG ACAGAGATCC TGGAAGAGAT ATGGGCATTT GGGGAAATAG   2067
TGTGACCATG TATCAGGCTT TGTGGACATC TAATGAGTAT GTAATGCTTA TGGAAGTAGA   2127
AGCATGCACG CAGAAACAAA GGTAGAAAAC TGCTTTGGGT GTTAACACTG TTCTCTGTCA   2187
CTATATAATA AAGAATACCT GCTGATGGCA                                    2217 
           
           
             
               2188 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             10
AAAGGAGTGA GTTGTGTACA GGGGGGTTAA ATGCTTTATA GACAAGAAAG AAATTGCTCT     60
AAAAGAGACT TATTCATCAT CATCATCATC TTCCTCCTCC TCTTCTTCCT CTTCTTCGTC    120
CTCTTCATCC TCTTCGTCTT CGTCCTCATC TTCCTCTTCT TCCTTCTTCT TCTTGCTCTT    180
CTCGGCCTTG GCAACTACTT TTTTGCCTGC ATCAACCTTC CCTTTGGCCC GGTATGCAGC    240
GATATCCTTC TCAGTCTCCT TCCTCTCTCT CCTTGGCCCA ACTCCTCCCC CCTCCTCTTC    300
TCCAGCACAG ATGGCCTTCA CATCGAGCTG CAACCACCCC AGTTTCACCC TCCCCTGGAG    360
GACCCTCCTG CCTTATCTCG TGGCTCTGCA CCACCTCCAG CCGGGATCAG CCCAGCTCAG    420
GGTGGTGGCA CCGAGCCTCC GTGTCACTGC CATTGTGGGA CAGGACGTCG TCTGCGCTGT    480
CACTTGTCTC CTTGCAAGAA TGCTTGGAAT TCAGACATCA GATGGATCCA GCACCGTTCC    540
TCTAGGATTG TGCACCACTA CCAAGACGGA GTGGACCTGG AGCAGATGGA GGAATATAAA    600
GGGAGGACAG AACTGCTCAG GGATGGTCTC TCTGATGGAA ACCTGGATTT GCGCATCACT    660
GCTGTGAGCA CCTCTGATAG TGGCTCATAC AGCTGTGCTG TGCAGGATGA TGATGGCTAT    720
GCAGAAGCTT TGGTGGAGCT GGAGGTGTCA GATCCCTTTT CCCAGATCGT CCATCCCTGG    780
AAGGTGGCTC TGGCTGTGAT CGTCACAATT CTGGTTGGGT CATCGGTCAT CATTGTTTTT    840
CTCTGTAGAA AGAAAGAGAG AAAAGATGGA GAGTTGGCGG AACAAGCTGA AATACTGGAG    900
AGAAAAGATG CAATGTTGAC GGAACAAGCT GAAACACTGG AGAAAAAAGA TGTAATGTTG    960
AAGGAACAAG CTATGATAGC GGAATCAAAT GCTGAAGATC TGAAGAAACT GGCTGCGAAA   1020
CTGGAGAAAC ACTCTGAAGA GATGGGGACA AGGGATTTAA AGTTGGATAA ATTAGCTGCC   1080
AAACTGGAAC ATCAAACTAA AGAATTGGAG AAACAGAAAT CGGAGCTGAA GAGTCACTTT   1140
CAGTATATGG ATTTCAATGC TGGAAAACAG AAGAAAATGG TTACAAAACT GGAGGAACAC   1200
TATGAATGGA TGGTGACAAG GAATGTAAAA TTGGAGATAC CAGCTATAAA AGTGGGGCAA   1260
CAAGCTAAAG AATCAGAGGA ACAGAAATCG GAGCTGAAGG AGCACCATGA GGAGATGGGG   1320
CAACAAGCTA AAGAATCAGA GGAACAGAAA TCGGAGCTGA AGGAGCACCA TGAGGAGATG   1380
GGGCAACAAG CTAAAGAATC AGAGGAACAG AAATCGGAGC TGAAGGAGCA CCATGAGGAG   1440
ATGGGGCAAC AAGCTAAAGA ATCAGAGGAA CAGAAATCGG AGCTGAAGGA GCACCATGAG   1500
GAGATGGGGC AACAAGCTAA AGAATCAGAG GAACAGAAAT CGGAGCTGAA GGAGCACCAT   1560
GAGGAGATGG GGCAACAAGC TAAAGAATCA GAGGAACAGA AATCGGAGCT GAAGGAGCAC   1620
CATGAGGAGA TGGGGCAACA AGCTAAAGAA TCAGAGGAAC AGAAATCGGA GCTGATGGTA   1680
GAAACTGAAG AAGCAGAAAA ACCATCTGAA GAATCAGATT GAGAGATGAA CTGCGCCTCC   1740
CAATAAGCAC AGGAGTTAAG CTTCATAGAT CAATGACTGT ACAGCAAACA AAAACCACGA   1800
TAACTCAAAC AGAGCAAGGA AATCCACAGC GAGAACAAGA AGAGCCAGTG TTTGTGTTGA   1860
GTGAGAACAC TGCAGTTCTG TCAGCCAAAG CTGTCTGAGG GACCGCCAAA TTGAGGGTGT   1920
CGAACCTCCA ACTCAAAGCC AATTGGAAGA AAGAAACCAT AGAAAGGAAG AAAAGGGGAG   1980
GGAGACAGAG ATCCTGGAAA AGATATGGGC ATTTGGGGAA ATAGTGTGAC CATGTATCAG   2040
GCTTTATGGA AATCTAACAA ATATGTCATG GTTTTGTAAA TACAAGCATG CACGCAGAAA   2100
CAAAGGTAGA AAACTGCTTT GGGTGTTAGC ACTGTTCTCT GTCCCTATAT AATAAAGAAT   2160
ACCTGCTGAT GGCAAAAAAA AAAAAAAA                                      2188 
           
           
             
               1487 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             11
TTGCAAGAAT GCTTGGAGCT TAGATATCAG ATGGATCCAG CTGCGGTCCT CTGGTTTTGT     60
GCACCACTAC CGAAATGGAG AGGACCTGGA GCAGATGACA GAATATAAAG GGAGAACAGA    120
ACTGCTCAGG AAGGGTCTTT CTGATGGAAA CCTGGATTTG CGCATCACTG CTGTGAGCAC    180
CTCCGATAGT GGCTCATACA GCTGTGTTGT GCAAGACGAT GATGGCTATG CAGAAGCGTT    240
GGTGGAGCTG GAGGTGTCAG ATCCCTTTTC CCAGATCGTC CATCCCTGGA AGGTGGCTCT    300
GGCTGTGATC GTCACAATTC TGGTTGGGTC ATTTGTCATC ATTGCTTTTC TCTATAGGAA    360
GAAAGCGACA CAGAGCAGAG AGCTGAAAAG AAAAGATGCA ATGTTGGGAA GAAAAGATGC    420
AGTGCTGGAG GAACTACCTG CGATATTAGA TTCAAGTGCT GCAAATCTGA AGATACTAGC    480
TTCAAAACTG GTGAAACAAA CTGAAAAATT GGACATACGG AATTCACTAA TGAAGAAACA    540
GTATGAAATG ACAGAGAAAC AAGCTGCAGA ACTGGAGAAA CACTTAATAA ATACCGATTT    600
AAGTGCTGCA GATCTGAAGA TAGCAGCTGC AAAACTGGAC AAACAAACTG AAGAACTGGA    660
CAAATGGAAA TCAGCACTGA AGATACAATA TGAAAAGTTG GGTTTACGTG CTGCAAATCT    720
GAAGACACAA GTTACAGAAC TGGCGAAACA AACTGAAGAA GTGGAAAATC ACTATGAAGA    780
GATGGGTTTA CGTGCTCCTA ATCTGAAGAA AAATATAGTA GAACTGGAGA AACAAACTGA    840
GCACGTGGAC AATCGGAAAT CAGAGCTGAA GAAACAGTAT GAAAATTTGG CTTCACATGC    900
TTCAGAGCTG AAGAAACAAG CTGAAGTACT GGAGGAACAA GCTGAACAAC TGGAGATTCA    960
GAATTCACTG TTGAAGATAC GCAATAAACA TAGGGAGAGA AAGAATGAAA TGTTGGAGAA   1020
ACAAACTGTA GAACAGGAAC AAACTGAAGA ATGGGCAGAA TCTAAAAAAT CGGTGGTTGA   1080
AACTAAAGAA TTGGAACAAC CATCTAAAGA ACAGGATTGA GAGATGAACT GCGCCTCACA   1140
GTAACCACAG GAGTTAAGCT TCATGGACTG CTGACTGCAC AGGATAGCAA CACCGCCATA   1200
ATGCAAAGCG AGCAAGGAAA TCCACAGCGA AAACAAGAGG AGCCAGTGTT TGTGTTGAGT   1260
GAGAACACTG CAGTTCCATG AGCCAAACCT GCCTGAGGGA CCGCCCAATT GAGGGTGTGC   1320
GACCTCCAAC TCAAAGCCAA TTGGAAGAAA GAAACCATAG AAAGGAAGAC TACAAGAGGA   1380
AGACAGAGAT CCTGGAAAAG GGATAGACAT TTTGGGATTT AACATGGCCA TGTATCAGGG   1440
TTTGAGGAAT TCTAACGTAT ATATAAGGCT TTTGGAAATA TAAACAT                 1487 
           
           
             
               4757 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             12
GGATGATCAT CCGACTCTTC TCATCATAAA TTCGTCTTCT TCTTTGCAGA GAAACTGGTT     60
ACAAAACTGG GTGAGTCCAA CCTCCCAAAC TAAATTAAAA GCAGTCAGAC TTTGTGAGCT    120
GTGGGATGAG ACGTTCTTCT CATCATGTGC TGCTTTCCTT TTACTTTTCC AGAGGAACAC    180
TTTGAATGGA TGGGTGAGTC TCCCCTCCCA AATTAAAAAT GTTGGGGTCT TCCTGTGTGA    240
GCTGTGGGAT GAGCTGTTCC TCCCATCATG CACTGGTTCT AATTTTCCTT TGCAGAGAGA    300
AGGAATGTAA AGTTGGGTGA GTCTTCTTCC CCAACCAAAG GGATTTGGGG TCTTCCATGG    360
GATCAGCCAT GGGATGATAA CCTGAACCTT ATCACATATT TCTTATTTGT TCTTTTTGCA    420
GAGATACCAG ATCTGTAATA CTGGGTGAGT CCTCCCTCCC AAATTAAATA CAAAAGGGGA    480
TCTGCCTGTG TGAGCTGTGG GATGAGATGT TCCTCTCATC ACGCATTATT TTCTCTTTCT    540
TTTCCAGGGC AACAAGCTAA AGAATCAGGT GAGTCTTCTT CCCTGTCCCA AAGGACTATG    600
GGTTTCCCAT GGGATGACAA GCTGTGCCAC CTCCTCACGA GGTGCTTCTT CTTTCTTTTT    660
TGCAGAGAAA CAGAAATCGG AGCTGAGTAA GTTGCAGTCA CTGAACTGAG GGAATGTGGG    720
GTCTTCCCAA AGTCTTGTGT ATGGGATGAA AAATCCCCTC TGACCATGCA CTGCTTTTCT    780
CCTCCTTTGC CAGAGGAGCG CCATGAGGAG ATGGGTGAGT CTCCCCTCCC ATATTAAAAT    840
CGTTGGGGTC TTCCTGTGTG AGCTGTGAGA TGAGATGTTC CTCTCATCAT GCGATGCTTT    900
TCTCTCTTTT CCAGCAGAAC AAACTGAAGC AGTGGGTGAG TCTTTGTCCC CAACCCAAAG    960
GAATATGGGG CAATCCATGG GATGACAAGC TGTCCCATCT CATCGTGCAT TGCTTTCCTA   1020
TTCCTTTTTT CTAGTGGTAG ATACTGAAGA AGCGGGTGAG TCTTTCCCAA ACCAAAGCAA   1080
TACGGGGTTT CCCATGGCAT GACAAGCTGT CCCACCTCAG CATCCGTTGT TTTTCTCTTT   1140
CTTTTCCAGA AAAACCATCT GAAGAATTGG ATTGAGAGAT GAACTGCGCC TCACAGTAAC   1200
CACAGGAGTT AAGCTTCATA GATCAATGAC TGCACAGCAT ACAAAAACCA CGATACCTCA   1260
AACAGAGCAA GGAAATCCAC AGCGAGAACA AGAGGAGCCA GTGTTTGTGT TGAGTGAGAA   1320
CACTGCAGTT CTGTCAGCCA AAGCTGCCTG AGGGACCGCC AAACTGAGGG TGTGCGACCT   1380
CCAACTCAAA GCCAATTGGA AGAAAGAAAC CATAGAAAGG AAGGAAAGGG GAGGAAGACA   1440
GAGATCCTGG AAGAGATATG GGCATTTGGG GAAATAGTGT GACCATGTAT CAGGCTGTGT   1500
GGACATCTAA CGAATATGTC ATGTTTTTGT AAATACAAGC ATGCACTCAG AAACAAAGGT   1560
AGAAAACTGC TTTGGGTGGT AACACTGTTC TCTGTCAAAA TATAATAAAG AATACCTGCT   1620
GATGGTAATG GATCATTGAT TGTGAGCAGT TATTGGGGTT TGGTTCCATG AAACAGGCTG   1680
AGTCTTCTTC CCAGAAACAA AGCAACGTGG GCTCTATCGG ATAACAAGCC GACCCTTCTC   1740
ACCATGCACT GCTATTCCAG CACAACAAGG CTCTCTCCAG GAAGCTAAAA AGGGATAAAA   1800
TAAATTAATA GGAAAGAAAT ACACAAAAAC AAGAAAATTT AAAAAAGAAT ACTCCAAAAA   1860
ATCTATAATT ATTACAATAA AAACTTTAAA AAAACACACC AACCTTCCAC CCTGGGGGAG   1920
CACCAATGAC AGCCTTTTGT GCCCCATCGC GGTTTTATGA GAACAGCCAC ACACTTCAGA   1980
GCTGACCCCG TGAGCCCCAC AGTGGGGGGA CCTCCCACAG TGGGTGGACC TCCTCCACAA   2040
CCACCCCCAT CACTCACATT GAATGCCCAA AGAAACAACA GCCCCAAAGG TTCCTCCTGG   2100
TGCTTCAGCC GCGTGTGTTC CTCATTCTGC TGTGCTGATG GTGATCATTA ACCCAACAGC   2160
TCATTAACCA GGTTATGGCT CAGGTGCGTG CTGCTGAACA AGCTTGGAGC CTAAAATGGT   2220
TCCTGCACAC ATCCCAGGGG ACGGCCCTCC ACCTTTCACT CCCCGCCATT ACAGCTCTCC   2280
TTAATCAGAG GAATACAGAT TCCATGCACT GAGTGCACTG AGCCATCGCC CACCTTCCCT   2340
ACAAACACCT CCTGGTCCCC ACAAACCCTC ACTGTGGGAA GAGGGGCTCT GGGGGGGTCA   2400
CAGGGACAAA CATTTAATAA TTCCTGTATT AATGGTTGAT TAACTTAAAA ATCTGTACTG   2460
ATCAAATAAA CTGCCACCCC TTGGGCATAG CTCAGAGCAT GCTCATGGAG TACAGCCCAC   2520
AGCTTTCCTC TGTGCTAGGG CAATGCTTCT CCTGGGTCCA TGTTCATCCT GGGTGGATGC   2580
AGAGCCCCAG GGTGGTACAT GAAACTGCAA TGGGATGTCA GTGTTCAGAG TTCTCCAACC   2640
GTCTGCCCCA TTGCCAAAGG GGTAAAGTTC CTCGGAGCAG ATTACCACAC CCTGGAGCTG   2700
GGCAAAGGTT GACGCTGGGC AAAGGTAGAA GCTGGGCATA GCTGCACGTT TCCTGCAGCT   2760
CAGGTGAGGG ATTTCTGTCT CTGTGGGGCT CCTTGTAGGG GAAATCCTTG GGGGGTCATC   2820
TGCTCTGCCT CACAGCCTGT GAGGAGCACT GGCACTGCCC AAGGCAGTGG TGGCTGTGCT   2880
CATGGAACTG ATGTTTGAGT GACCCCATCC CCTCCTCTCC TGGTGGCTGT AACCCTCTGG   2940
CCCCTCTCCT CCTACAGCTC CTTCCTGCAT ATTCTTCCTC AACTTTTTCT AAATCTTCTT   3000
TCCAAATCTT CTACCCCATC TGCTCCAGCA CCTCCTTCTC CATCTCCTTC CCCAAACTCC   3060
TCCTTATATC CCCTTCCCCA ATCTCCTTCA CCCACCTCCT TCTCCTATCA TCTTCTCTCA   3120
TCTTTTACCA TTTTCTACCC ACCTTCTGCC CCATCTCCTC CATCATATCC TTCTCAGTCT   3180
CCTTCCTCTC TCTCCTTTCC CCAACTCCTT CCCCCCTCCT CTTCTCCAGC ACAGATGGCC   3240
TTCACATCGA GCTGCAACCA CCCCAGTTTC ACCCTCCCCT GGAGGACCCT CCTGCCTTAT   3300
CTCGTGGCTC TGCACCACCT CCAGCCGGGA TCAGGTAGGG GTCCTGTGGG GCTGCTGTGC   3360
CTGGCACACG TGTTGCTATG GGGTGGGGGA GCCGCCATGG GGCAGGGAGG ACACAAGTCC   3420
AGCCCCCAGC CCCACTTGGG TTTCACTTTC ACTTTGGTAA TTCCATGATA GATGCCATTT   3480
TGGGTAGAAT TTCTGTCTCT TCTTCACCTC TGCCACACGG TGTGAGTGGG CTCCCACCCC   3540
CAGCAATCCT TCCCCCTCTC TCCTGATCCC TCCCCACTGC TTTTACACCA GATGGAGCAC   3600
ACACCAACTC ACCCTGTGCC GCTCCATGCC CCCACATTAA CACAGACACC ATCTCACCAT   3660
CTCTCCGTGC CCTTCGCATT GCCCAGCCCA GCTCAGGGTG GTGGCACCGA GCCTCCGTGT   3720
CACTGCCATT GTGGGACAGG ACGTCGTCTG CGCTGTCACT TGTCTCCTTG CAAGAATGCT   3780
TGGAATTCAG ACATCAGATG GATCCAGCAC CGTTCCTCTA GGATTGTGCA CCACTACCAA   3840
GACGGAGTGG ACCTGGAGCA GATGGAGGAA TATAAAGGGA GGACAGAACT GCTCAGGGAT   3900
GGTCTCTCTG ATGGAAACCT GGATTTGCGC ATCACTGCTG TGAGCACCTC TGATAGTGGC   3960
TCATACAGCT GTGCTGTGCA GGATGATGAT GGCTATGCAG AAGCTTTGGT GGAGCTGGAG   4020
GTGTCAGGTC AGTGGCTGGG GTGACGTCTC CAGGTGTCCC TGGGTTTGTG GGTCCCACCC   4080
AACCTCTGTC CATCCTCATC CTCACGTCCA TGGATGGAGA GCTGAAGGAC AGCAGCCTTT   4140
GGAAGAGGTC AGGGCTGAAT TGTTTTATGA GATGCTGGAA TTAGAGCGGA CACACGGTGT   4200
GATTTGGGGA ATAGACTGCA TGGATGAGGT GGTTGGGTTG GATTTCTGGG ATGGGTTTCT   4260
CCATGTATCA GTGGCAGTGG GCACACGATG CTGAGCAGCT CCTCCGCCTG TGCCAATATG   4320
GGGACGCTGC CATTGTGTGT CACTGTTCCC TGCTCACTGC TCCTTCTGAA CAGGTGAATT   4380
CCGTTACCTT TTCCTTGGGA ACAGGACTAC AAAAAAGGTC TAGGGAAAAG GGTCTAGCAG   4440
GTAGGGACCT TCCACCGAGA CCGACACTAG CAGTGTTAAG ACCAACCCAG TAGCCAGTAG   4500
TAACAAAAAG AGACATCTTT CTTTCCACTC AACTCGTACC TCCCCTACCT CGTGTCCTTC   4560
CACAACACGT ACCTGTCCTT ACCAGCCCCA CCACGACTCG AGTCCAGGTG TCTCCATGTG   4620
TCCTCCTGCT TCCCTCTAAA AAGGACTCTA AGGGTCACGA GTAATTTATT GAAAAGGGAA   4680
AGAAAAACCC TTACTTCCTT CCTTTTTTTC CCCACACCCA CCCTTCTATC CTTACACCGA   4740
CATCCGTCCA CCTTTCA                                                  4757 
           
           
             
               35 amino acids 
               amino acid 
               single 
               Not Relevant 
             
             
               peptide 
             
             N-terminal 
             
               unknown 
             
             13
Pro Ala Val Lys Val Gly Gln Gln Ala Lys Glu Ser Glu Glu Gln Lys
1               5                   10                  15
Ser Glu Glu Met Gly Thr Arg Asp Leu Lys Leu Glu Arg Leu Ala Ala
            20                  25                  30
Lys Leu Glu
        35 
           
           
             
               35 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             N-terminal 
             
               unknown 
             
             14
Pro Ala Val Lys Leu Gly Gln Gln Ala Lys Glu Ser Gly Lys Gln Lys
1               5                   10                  15
Ser Ala Asn Ser Gly Val Ala Asp Leu Lys Glu Leu Ala Ser Glu Leu
            20                  25                  30
Tyr Asp Glu
        35 
           
           
             
               35 amino acids 
               amino acid 
               single 
               linear 
             
             
               peptide 
             
             N-terminal 
             
               unknown 
             
             15
Pro Ala Val Ile Leu Gly Gln Gln Ala Lys Glu Ser Glu Glu Gln Lys
1               5                   10                  15
Ser Glu Gly Ser Gly Val Ala Asp Leu Lys Leu Ala Ala Lys Leu Glu
            20                  25                  30
Tyr Ile Ala
        35 
           
           
             
               7350 base pairs 
               nucleic acid 
               double 
               linear 
             
             
               DNA (genomic) 
             
             
               unknown 
             
             16
CTGGGTCAGA TCTCCCGGCT TCATTTCTCT CCATCCCTGG GGTCCCCTCC TCCCGTCTGA     60
CTGCTGGAGG GCGGATGATC ACCCCCTGTC TGCACCCCTC CCTGCGCTAT CTGCAGCCCT    120
TCAGATGCAC CGCACCCCAT TTGCACTCCC TGCCCCCCCT TTGTACACAT GGGGGGGATA    180
TCAGCCCTCC TCCTTCCACC CACCCGTATC AGAGCCGCTG TGCTGCTGAG GGAGGCGGAT    240
GGGACGGCTG CATCGCTCCC CCTCAGCTTC ACAGAGCTGC TTTGCTGCGG GTTTTGGCTG    300
CAATTCGGAC CCTCTAAGAA TGATCCCTCG TTGTGAGACT CCGCTGCAAA GCTGATCCGT    360
TCGAGCTCTC CTCCTACAGC TGCTGCCCTC ATATTCTCCC CACACTTCTT CCCCATATTC    420
TTTCCAAATC CTCTTCCCCA TCTCCTCCAC CGTCTCTTTC TCAGAGTCCT TCCTCTCTCT    480
CCCTAAATTC TTCCCCCCTC CTCTCCTCCA GCACAGATGC GCTTCACATC GGGATGCAAC    540
CACCCCAGTT TCACCCTCCC CTGGAGGACC CTCCTGCCTT ATCTCGTGGC TCTGCACCTC    600
CTCCAGCCGG GATCAGGTAG GGGTCCTGTG GGGCTGCTGT GCCTGGCACA GGTGTTGCTG    660
TGGGGTGGGG GAGCAGCCAT GGGGCAGGGA GGACCCATGT CCAGCACCCA GCCTCGCTTG    720
GGTTTCTCTT TCACTTGGGC TATTTCATGA AATGTGTGAT TTCGGGTGGA ATTTCTGTCC    780
CTTCTTCACC TCCACCACAC GGTGTGAGTG GGCTCCCACC CCCAGCAATC CTTGCCCACT    840
CCCTCCTGAT CCCTCCCCAC TGCTTTTACA TGGGATGGAG CACACACCAA CTAACCCTGT    900
GCCGCTCCAT GCCCCCACAT TAACACAGCC ACCATCTCAC CATCTCTTCG TGCCCTTCTC    960
ATTGCCCAGC CCAGCTCAGG GTGGTGGCGC CGAGCCTCCG TGTCACTGCC ATCGTGGGAC   1020
AGGATGTCGT GCTGCGCTGC CACTTGTGCC CTTGCAAGGA TGCTTGGAGA TTGGACATCA   1080
GATGGATCCT GCAGCGGTCC TCTGGTTTTG TGCACCACTA TCAAAATGGA GTGGACCTGG   1140
GGCAGATGGA GGAATATAAA GGGAGAACAG AACTGCTCAG GGATGGTCTC TATGATGGAA   1200
ACCTGGATTT GCGCATCACT GCTGTGAGCA CCTCCGATAG TGGCTCATAC AGCTGTGCTG   1260
TGCAGGATGG TGATGGCTAT GCAGACGCTG TGGTGGACCT GGAGGTGTCA GGTCAGTGGC   1320
TGGGGTGATG TCTCCAGGTG TCCCTGGGCT TGTGTGTCCC CTACCGACCT CTGTCCATCC   1380
TCATCCTCAC ATCCTAGGAT GGAGAACTGA AGGACAGCAG CCTTTGGAAG AGCTCAGGGC   1440
TGAACAGCTC CATGAGATGC TGGAGTTGGA TCGGGCACAT GGTGTAATTT GAAAATGGAT   1500
ATGCATGGAT GAGGTGGTTG GGTTGGGTTT CTGGGATGGG TTTCTCCACG TCTCAGTGGC   1560
AGTGGGCACA CGATGCTGAG CAGCTCCTCC GCCTGTGCCA ATATGGGGAC GCTGCCATTG   1620
TGTGTCACTG CTCCCTGGTT GTTGTCCCTT CGGGTTCTGT GATCTCCAGA AGTCGAAGTC   1680
GTGTTTGTCC ACATAAGGCA GTGGAAAAAG GAACCCTTGT CCTGATGTCT TTTCCAGATC   1740
CCTTTTCCCA GATCGTCCAT CCCTGGAAGG TGGCTCTGGC TGTGGTCGTC ACAATTCTCG   1800
TTGGGTCATT TGTCATCAAT GTTTTTCTCT GTAGGAAGAA AGGTGAGCTG AGAGCGGAGG   1860
GGATGGAGCA CAGGGAGGTG TTGTGCATGG ACAGGGATGG TCGGGGTGGT GCTGAGCTCT   1920
GGTGTACAGA GGTACACAGG AGGAGAAAGG GAGATTTTTC CTGACATTCC CACTGCCCAT   1980
TAAATAACAT TGCCTTTCTT TTGGGGAAAT GAAGGAGGAA AAAAAGAAGT GTGGGTGGGC   2040
AGATAGGAAA GTGGGTGGAC CGTGGGGCAG GTGGAAAGGT CCAGACCTCG GGACGTCCCC   2100
AAACCAAGCT GCCCTGCTGA CTACCTCTTC CTCCAATTTG TTTTCCAGCG GCACAGAGCA   2160
GAGAGCTGAG TGAGTCCTTC CAGCCCCTTC CACCACCAAA GTCCCTTTAA TGGAACTGAT   2220
AGAAGACTGC AGAGTGCTGG GTTTATGCCT TGTGCTGGGG CCATGGGATC TATGGGACCT   2280
TGGGATGTGT TGGGGCCGTG GGATGTGCTG GGGTCGTGGG ATCTGTCAAC CCTGATTGAT   2340
CCACTTCAGA ACTCTTGCCC AATCGGTTCC TTCCGATTCA TTTAACTCCT TCTTGAGGCC   2400
AAAGTGGTCA TTGGCCACAT CCCAGAAAAA AGGGTTTGGG GTCAGGGTGT GGGAGCTGAT   2460
CGCATGGAAA CGTGTCCCCT CTGACCATGC ATTTCATTTG CTTCTATTTT GCAGAGAGAA   2520
AACATGCAGC GTTGGGTAAG TCTCCTCCCC ATATGTGAGG GAATTCAGGG TGTCCCCATG   2580
GCATCAGCAG TGGGATGAGC AGCTGTCCGC TCTGACCATG CACTGCTCTG CTCTTTCTTT   2640
TCCAGCGGAA CTAGATGAGA TATCGGGTGA GTCTCCATTC CCAATTGTAT TCTTTCAAAT   2700
GTTCTGCCTT GGGGAGCTGT GGGATAGGAT GTTCTTCTCA CCATGCACTG ATTCTACCTT   2760
TCCATTGCAG GTTTAAGTGC TGAAAATCTG AGTAAGTGTC CCTCCTGACA CTGAAGGAAT   2820
TTGGGGTATT CCCATGGGAT CAGCCATTGA ATGAAAACAT GGCCCCCTCT CTTCATGCAT   2880
TTCCTATTTC TTACCTTTGC AGAGCAATTA GCTTCAAAAC TGAGTGAGTG CTCACTCCCA   2940
AACTCAAAGT AAAGAGAGTC TGCCTGTGTG AGCTGTGGGA TGAGATGTTC CACTCATCGT   3000
GCATTGCTTT TCTCTTTATT TTCCAGACGA AAATGCTGAC GAGTGGGTGA GTCTACATTC   3060
ACTAATGCAA AGAAATATGG GGTCTCCCAA GGGATGACAA GCGTGTCCCG CATCATCATT   3120
TGGTGCTTCT TCTGTCTTTT TTTTTGCAGA GGATTGCAAT TCAGAGCTGA GTAAGTTGCA   3180
GTCACTGAAC TGAGGGAATG TGGGGTCTTC CCAAGGGACA GTGCATGGGA TGAAAAATCC   3240
CCTCTGACCA TGCACTGCTT TTCTCTTTCT TTCCCAGAGA AAGACTGTGA AGAGATGGGT   3300
GAGTCCCCCC CCCCAAAATT AAACGTTGGG GTCCTCATGT GGAGCTGTGG ATGAGATGTC   3360
CTCTCATCAC GCACTGTTTC TACATTTCTT TGCAGGTTCT GGCGTTGCAG ATCTGAGTAA   3420
GTCTCCCCTA CCAGCACGGA AGGAATTTGT GGTCTTCCCA TGGGATCAGC CATGGGACTG   3480
ATCATCTGAG CCCTCTCATC ATGCATTTCA TATTCGTTCC TTTTGCAGAG GAACTGGCTG   3540
CAAAATTGGG TGAGTGTTGC CTCCCAAATT AAATTAAAAA AGGGGGTCTG CCTGGGCTCG   3600
CTGTGGGATA GGATCTTCCT CTCACTGTGT GTTGCTTTTC CCTTTCTTTT CCAGAGGAAT   3660
ATATTGCAGT GAATCGTGAG TCTCCCCTCC GAAATTATAA ATGCTGGGGA AATCTTGTGT   3720
GCGATCGTGG GTAGAGCTCT TCCTCTCATC ATGCACTGTT TCTGCTTTTC CTTTGCAGGG   3780
AGAAGGAATG TAAAGTTGAG TGAGTCTCTC TTCCCAAACC AAACAGATTT GGGGTCTTCC   3840
CATGGGATCA GCCATGGGAT GATAATCTAA CCCTACTCAT CATGCATTTC TTATTGGTTC   3900
CTTTGGCAGA TAATATAGCT GCCAAACTGG GTGAGTCCCC CCTCACAGAT TACATAAAAA   3960
ATGGGGTCTG CCTGTGTGAG CTGTGGGATG AGATGTTCCT CTCATCATGT ACTACTTTTC   4020
TCTTCCTTTT CCAGCACAAC AAACTAAAGA ATTGGGTGAG TCTTCTTTCC CCAAACAAAG   4080
AAATACGGGA TTCCCATGGG ATGACAAGCT GTGCCACCTC ATCATGCCCT GTTTTTTCTG   4140
TCCTTTTTGC AGAGAAACAG CATTCACAGT TCCGTAAGTT GCAGTCACTA AACTGAAGGA   4200
ATGTGGGGTC TTCCCAAAGT CCTGCATACG GGATGAAAAA TCCCCTCTGA CCATGCACTG   4260
CTTTTCTCTT TCTATTCCAG ACAGACACTT TCAGCGTATG GGTGAGTCTC TCCCCCCCAA   4320
ATTAAAAACG CTGGGGGCAT CCTATGGGAG CTGTGGGATG AGATTTTCCT CTCATCACAC   4380
ACTCCTTCTG CTTTTCCATT GCAGATTTAA GTGCTGTAAA CCAGAGTAAG TCTCCCTCCC   4440
TGCACAGAAG GAACTTCCAG TTTTCCCATG GGATCAGCCA TGGGATGATC ATCCGACTCT   4500
TCTCATCATA AATTCGTCTT CTTCTTTGCA GAGAAACTGG TTACAAAACT GGGTGAGTCC   4560
AACCTCCCAA ACTAAATTAA AAGCAGTCAG ACTTTGTGAG CTGTGGGATG AGACGTTCTT   4620
CTCATCATGT GCTGCTTTCC TTTTACTTTT CCAGAGGAAC ACTTTGAATG GATGGGTGAG   4680
TCTCCCCTCC CAAATTAAAA ATGTTGGGGT CTTCCTGTGT GAGCTGTGGG ATGAGCTGTT   4740
CCTCCCATCA TGCACTGGTT CTAATTTTCC TTTGCAGAGA GAAGGAATGT AAAGTTGGGT   4800
GAGTCTTCTT CCCCAACCAA AGGGATTTGG GGTCTTCCAT GGGATCAGCC ATGGGATGAT   4860
AACCTGAACC TTATCACATA TTTCTTATTT GTTCTTTTTG CAGAGATACC AGCTGTAATA   4920
CTGGGTGAGT CCTCCCTCCC AAATTAAATA CAAAAGGGGA TCTGCCTGTG TGAGCTGTGG   4980
GATGAGATGT TCCTCTCATC ACGCATTATT TTCTCTTTCT TTTCCAGGGC AACAAGCTAA   5040
AGAATCAGGT GAGTCTTCTT CCCTGTCCCA AAGGACTATG GGTTTCCCAT GGGATGACAA   5100
GCTGTGCCAC CTCCTCACGA GGTGCTTCTT CTTTCTTTTT TGCAGAGAAA CAGAAATCGG   5160
AGCTGAGTAA GTTGCAGTCA CTGAACTGAG GGAATGTGGG GTCTTCCCAA AGTCTTGTGT   5220
ATGGGATGAA AAATCCCCTC TGACCATGCA CTGCTTTTCT CCTCCTTTGC CAGAGGAGCG   5280
CCATGAGGAG ATGGGTGAGT CTCCCCTCCC ATATTAAAAT CGTTGGGGTC TTCCTGTGTG   5340
AGCTGTGAGA TGAGATGTTC CTCTCATCAT GCGATGCTTT TCTCTCTTTT CCAGCAGAAC   5400
AAACTGAAGC AGTGGGTGAG TCTTTGTCCC CAACCCAAAG GAATATGGGG CAATCCATGG   5460
GATGACAAGC TGTCCCATCT CATCGTGCAT TGCTTTCCTA TTCCTTTTTT CTAGTGGTAG   5520
ATACTGAAGA AGCGGGTGAG TCTTTCCCAA ACCAAAGCAA TACGGGGTTT CCCATGGCAT   5580
GACAAGCTGT CCCACCTCAG CATCCGTTGT TTTTCTCTTT CTTTTCCAGA AAAACCATCT   5640
GAAGAATTGG ATTGAGAGAT GAACTGCGCC TCACAGTAAC CACAGGAGTT AAGCTTCATA   5700
GATCAATGAC TGCACAGCAT ACAAAAACCA CGATACCTCA AACAGAGCAA GGAAATCCAC   5760
AGCGAGAACA AGAGGAGCCA GTGTTTGTGT TGAGTGAGAA CACTGCAGTT CTGTCAGCCA   5820
AAGCTGCCTG AGGGACCGCC AAACTGAGGG TGTGCGACCT CCAACTCAAA GCCAATTGGA   5880
AGAAAGAAAC CATAGAAAGG AAGGAAAGGG GAGGAAGACA GAGATCCTGG AAGAGATATG   5940
GGCATTTGGG GAAATAGTGT GACCATGTAT CAGGCTGTGT GGACATCTAA CGAATATGTC   6000
ATGTTTTTGT AAATACAAGC ATGCACTCAG AAACAAAGGT AGAAAACTGC TTTGGGTGGT   6060
AACACTGTTC TCTGTCAAAA TATAATAAAG AATACCTGCT GATGGTAATG GATCATTGAT   6120
TGTGAGCAGT TATTGGGGTT TGGTTCCATG AAACAGGCTG AGTCTTCTTC CCAGAAACAA   6180
AGCAACGTGG GCTCTATCGG ATAACAAGCC GACCCTTCTC ACCATGCACT GCTATTCCAG   6240
CACAACAAGG CTCTCTCCAG GAAGCTAAAA AGGGATAAAA TAAATTAATA GGAAAGAAAT   6300
ACACAAAAAC AAGAAAATTT AAAAAAGAAT ACTCCAAAAA ATCTATAATT ATTACAATAA   6360
AAACTTTAAA AAAACACACC AACCTTCCAC CCTGGGGGAG CACCAATGAC AGCCTTTTGT   6420
GCCCCATCGC GGTTTTATGA GAACAGCCAC ACACTTCAGA GCTGACCCCG TGAGCCCCAC   6480
AGTGGGGGGA CCTCCCACAG TGGGTGGACC TCCTCCACAA CCACCCCCAT CACTCACATT   6540
GAATGCCCAA AGAAACAACA GCCCCAAAGG TTCCTCCTGG TGCTTCAGCC GCGTGTGTTC   6600
CTCATTCTGC TGTGCTGATG GTGATCATTA ACCCAACAGC TCATTAACCA GGTTATGGCT   6660
CAGGTGCGTG CTGCTGAACA AGCTTGGAGC CTAAAATGGT TCCTGCACAC ATCCCAGGGG   6720
ACGGCCCTCC ACCTTTCACT CCCCGCCATT ACAGCTCTCC TTAATCAGAG GAATACAGAT   6780
TCCATGCACT GAGTGCACTG AGCCATCGCC CACCTTCCCT ACAAACACCT CCTGGTCCCC   6840
ACAAACCCTC ACTGTGGGAA GAGGGGCTCT GGGGGGGTCA CAGGGACAAA CATTTAATAA   6900
TTCCTGTATT AATGGTTGAT TAACTTAAAA ATCTGTACTG ATCAAATAAA CTGCCACCCC   6960
TTGGGCATAG CTCAGAGCAT GCTCATGGAG TACAGCCCAC AGCTTTCCTC TGTGCTAGGG   7020
CAATGCTTCT CCTGGGTCCA TGTTCATCCT GGGTGGATGC AGAGCCCCAG GGTGGTACAT   7080
GAAACTGCAA TGGGATGTCA GTGTTCAGAG TTCTCCAACC GTCTGCCCCA TTGCCAAAGG   7140
GGTAAAGTTC CTCGGAGCAG ATTACCACAC CCTGGAGCTG GGCAAAGGTT GACGCTGGGC   7200
AAAGGTAGAA GCTGGGCATA GCTGCACGTT TCCTGCAGCT CAGGTGAGGG ATTTCTGTCT   7260
CTGTGGGGCT CCTTGTAGGG GAAATCCTTG GGGGGTCATC TGCTCTGCCT CACAGCCTGT   7320
GAGGAGCACT GGCACTGCCC AAGGCAGTGG                                    7350