Abstract:
Disclosed are a deodorizer using an autotrophic microbe, and a method for preparing the same. The deodorizer is prepared through a culturing step for amplifying an autotrophic microbe of interest, a sterilizing and purifying step for removing impurities and harmful microbes other than the autotrophic microbe, and a harvesting step for collecting the filtered autotrophic microbes. The microbial deodorizes is effective for removing odorous emissions attributed to ammonia, trimethylamine, formaldehyde, and hydrogen sulfide and is safe to the body and the environment including air and water.

Description:
CROSS-REFERENCE TO RELATED U.S. APPLICATIONS 
       [0001]    Not applicable. 
       STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT 
       [0002]    Not applicable. 
       NAMES OF PARTIES TO A JOINT RESEARCH AGREEMENT 
       [0003]    Not applicable. 
         [0004]    REFERENCE TO AN APPENDIX SUBMITTED ON COMPACT DISC 
         [0005]    Not applicable. 
       BACKGROUND OF THE INVENTION 
       [0006]    1. Field of the Invention 
         [0007]    The present invention relates to a deodorizer using an autotrophic microbe. More particularly, the present invention relates to a microbial deodorizer which is effective for removing odorous emissions attributed to ammonia, trimethylamine, formaldehyde, and hydrogen sulfide and which is safe to the body and the environment including air and water because it undergoes a sterilization and purification step when boiled at 99° C. for 5 min. 
         [0008]    2. Description of Related Art Including Information Disclosed Under 37 CFR 1.97 and 147 CFR 1.98. 
         [0009]    Foul odor from living environments, such as domestic sewage, excreta, furniture, facilities, constructs, pets, etc. is generally accounted for by nitrogen compounds, sulfides, aldehydes, and other carbon compounds. Representative among the causes of bad odor are ammonia, trimethylamine, formaldehyde, hydrogen sulfide, and hydrocarbons. Typically, air purifiers, chemical deodorizers, and microbial deodorizers are used to remove foul odors. 
         [0010]    Due to their artificial and synthetic chemicals, chemical deodorants however, emit ingredients detrimental to the body, causing the production of air pollutants. That is, chemical deodorizers, although effective for deodorization, destroy the virtuous cycle of the natural ecosystem in a long-term aspect. 
         [0011]    Microbial deodorizers exhibit rather adverse effects because intensive sterilization for eliminating contaminants or putrefactive microbes, which may be introduced or generated during manufacturing processes, cannot be carried out lest the main microbes would be killed. 
         [0012]    For example, Korean Patent No. 10-1114185 discloses a deodorant composition comprising a fermentation product fermented from fermentation materials including a rice bran extract, a sugar and solar salt by an effective microorganism (EM), plus the plant surfactant cocobetaine and a hot water extract of rosemary, and a manufacturing method thereof. 
         [0013]    Because it is based on a heterotrophic microorganism, this deodorant composition cannot be subjected to a sterilization process for removing detrimental impurities or microbes other than the effective microorganism. Thus, it is not recommended to elicit the deodorization effect by applying the deodorant composition directly to living bodies, such as humans, animals or plants. 
         [0014]    Korean Patent. No. 10-0958064 addresses a microbial agent for wastewater purification and a purifying device comprising the same, which are configured to improve both foul odor and water quality, with the aim of solving problems with organic wastewater disposal such as high expense and low efficiency, and coping with strengthened environmental regulations. 
         [0015]    This microbial agent is produced without any sterilization or purification process for removing detrimental microbes or impurities other than working microbes, so that it is not recommended to be directly sprayed to air, surroundings, or human or animal bodies. 
         [0016]    Keeping in mind the above problems encountered in the prior art, the present invention has been made by selectively utilizing autotrophic microbes that are believed to purify the atmosphere of the proto-earth to establish an environmental background for the appearance of higher organisms, such as humans, animals and plants, that is, by culturing the autotrophic microbes, and performing sterilization and purification to remove impurities and microorganisms other than the microbes of interest to afford an environment-friendly microbial deodorizer product which can effectively remove odorous emissions from household sources in sympathy with the virtuous cycle of the ecosystem and which is harmless to the body and the environment. 
       PRIOR ART DOCUMENTS 
     Patent Documents 
       [0017]    1. Korean Patent No. 10-114185 
         [0018]    2. Korean Patent No. 10-0958064 
       BRIEF SUMMARY OF THE INVENTION 
       [0019]    It is therefore an object of the present invention to provide an environment-friendly microbial deodorizer using an autotrophic microbe which can effectively remove various odorous emissions from living environment sources, in harmony with the virtuous cycle of the ecosystem without harm to the body and the environment. 
         [0020]    It is another object of the present invention to provide a process for manufacturing the environment-friendly microbial deodorizer. 
         [0021]    To achieve the above objects, the microbial deodorizer using an autotrophic microbe in accordance with the present invention is manufactured by a process comprising a screening step (S1), a culturing (S2-1) and mixing (S2-2) step, a sterilizing step (S3), a purifying step (S4), and a harvesting step (S5). 
         [0022]    In the screening step (S1), a purple sulfur bacterial strain, found in soil, is selected from the group consisting of  Allochromaium palmeri, Ectothiorhodosinus mongolicus, Halochromatium roseum,  and a combination thereof. 
         [0023]    The second step may be divided into sub-steps of culturing (S2-1) and mixing (S2-2). The autotrophic microbial strain selected in the screening step is cultured in a medium, adjusted to a pH of 6.0-7.0, containing potassium phosphate, magnesium sulfate, sodium chloride, ammonium chloride, and calcium chloride in an incubator at 43-45° C. for 190-210 hrs under the light of 5000 Lux or higher (S2-1), the autotrophic bacteria amplified above are adjusted to a density of 4-5×10 5  cfu/ml with, as needed, a distilled water (S2-2). 
         [0024]    Next, the sterilizing step (S3) is carried out by boiling the diluted culture of the autotrophic bacteria at 99° C. for 5 min so as to remove other bacteria and contaminants which could be introduced during the above steps. 
         [0025]    After the sterilization, the microbial culture is cooled to room temperature in a clean room and incubated for 48-60 hrs without feeding an additional medium thereto, followed by filtration (purifying step S4). 
         [0026]    Finally, the autotrophic microbe filtrate is maintained at a density of 4-5×10 5  cfu/ml and processed into a commercial product (harvesting and commercializing step (S5). 
         [0027]    An examination was made of the ability of the microbial deodorizer of the present invention to deodorize ammonia, trimethylamine, formamide, and hydrogen sulfur by the Korea Conformity Laboratories. 
         [0028]    The microbial deodorizer of the present invention was found to have faster and improved deodorization, compared to conventional microbial agents prepared without sterilization and purification, as demonstrated by the testing of the authorized office (Tables 1-8). 
         [0029]    As described hereinbefore, the present invention is characterized by the sterilization and purification of microbial agents, for example, those of Korea Patent No. 10-0958064, in order to make them harmless to the body and the environment without degrading the deodorizing ability. In a fish bowl filled with the microbial deodorizer of the present invention, gold fish were safely grown for three months ( FIG. 3 ). In addition, the microbial deodorizer of the present invention was found to be safe to the body and the environment because of no detection of heavy metals and other harmful substances therefrom, as described in a test report prepared by the Korea Conformity Laboratories (Table 9). 
     
    
     
       BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS 
         [0030]    The above and other objects, features and advantages of the present invention will be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings, in which: 
           [0031]      FIG. 1  is a schematic view illustrating the degradation of odorous sources into non-odorous compounds by autotrophic microbes according to the present invention. 
           [0032]      FIG. 2  is a flow chart, showing the manufacturing process of a microbial deodorizer of the present invention in which an autotrophic microbe is screened (S1), cultured and mixed (S2), sterilized (S3), purified (S4), and harvested (S5). 
           [0033]      FIG. 3  is a photograph illustrating the safety of the microbial deodorizer which underwent the sterilizing (S3) and purifying step (S4), as demonstrated by the healthy growth of gold fish exposed thereto. 
           [0034]      FIG. 4  is a picture taken of a commercial product of the autotrophic microbe according to the present invention. 
           [0035]      FIG. 5  is a graph showing deodorizing effects on ammonia gas of two microbial agents which are prepared by conducting up to the culturing step (S2), and up to the sterilizing (S3) and purifying step (S4), respectively. 
           [0036]      FIG. 6  is a graph showing deodorizing effects on trimethylamine gas of two microbial agents which are prepared by conducting up to the culturing step (S2), and up to the sterilizing (S3) and purifying step (S4), respectively. 
           [0037]      FIG. 7  is a graph showing deodorizing effects on formaldehyde gas of two microbial agents which are prepared by conducting up to the culturing step (S2), and up to the sterilizing (S3) and purifying step (S4), respectively. 
           [0038]      FIG. 8  is a graph showing deodorizing effects on hydrogen sulfide gas of two microbial agents which are prepared by conducting up to the culturing step (S2), and up to the sterilizing (S3) and purifying step (S4), respectively. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0039]    Below, a detailed description will be given of the present invention. 
         [0040]    In accordance with an aspect thereof, the present invention addresses a microbial deodorizer. 
         [0041]    For use in the microbial deodorizer of the present invention, a purple sulfur bacterial strain is selected from  Allochromatium palmeri, Ectothiorhodosinus mongolicus, Halochromatium roseum,  and a combination thereof. (Screening step S1). 
         [0042]    That is, the three bacterial strains may be employed alone, or in combination in the microbial deodorizer of the present invention. 
         [0043]    For use as a culture medium for the bacterial strains, potassium phosphate, magnesium sulfate, sodium chloride, ammonium chloride, and calcium chloride are mixed and dissolved in pure water and the solution is adjusted to a pH of 6.0-7.0. The bacterial strains are cultured in the medium in an incubator at 43-45° C. for 190-210 hrs under the light of 5000 Lux or higher until they are grown to a density of 4-5×10 5  cfu/ml or greater (Culturing step S2-1 and mixing step S2-2). 
         [0044]    Nitrogen, sulfur, aldehyde and hydrocarbon compounds, such as ammonia, trimethylamine, formaldehyde, hydrogen sulfide, etc. are predominantly responsible for odorous emissions from surrounding environment sources, such as household sewage, facilities, constructions, pets, etc. 
         [0045]    Nitrogen, sulfur, and carbon compounds are fermented or degraded by the autotrophic microbes of the present invention, as exemplified as follows. 
         [0000]      NH 4   + →NO 2 →NO 3 →N2
 
         [0000]      H 2 S→SO or SO 4   30  
 
         [0000]      C 6 H 12 O 6 →CH 3 COOH or Alcohol→CH 4  or CO 2 +H 2 O
 
         [0046]    In addition to ammonia and hydrogen sulfide, there are other nitrogen and sulfur compounds that emit foul odors in living environments, examples include methylamine, ethylamine, dimethylamine, trimethylamine, isobutylamine, isoamylamin, phenylamine, putrescine, and cadaverine as nitrogen compounds, and methyl mercaptan, ethyl mercaptan (C 2 H 3 SH), dimethyl sulfide ((C 3 ) 2 S), diethyl sulfide ((C 2 H 3 ) 2 S), and dimethyl disulfide (CH 3 S═SCH 3 ) as sulfide compounds. These nitrogen and sulfur compounds are removed through the degradation mechanism above. 
         [0047]    Among other carbon compounds serving as sources of odorous emissions are aldehydes and ketones (formalin, acetaldehyde, butylaldebyde, acrolein, acetone, acrylaldehyde), aliphatic acids (butyric acid, lactic acid, etc.), hydrocarbons (styrene, butyrene, etc.), chlorohydrocarbons (trichloroethylene, tetrachloroethylene, acrylic acid ester, acetic acid ester). These can be degraded in a similar mode of mechanism. 
         [0048]    The break-down products concomitantly produced during the removal of odorous ingredients by the purple sulfur bacteria act to inhibit the growth of the putrefactive and pathogenic microorganisms activated by various pollution sources. 
         [0049]    As well as functioning to deodorize odorous emissions from air and water, the microbial agent of the present invention is safe to the body and the environment, as manufactured through sterilization and purification steps for removing impurities and harmful microorganisms from the culture medium in which the autotrophic microbes of the present invention are cultured. 
         [0050]    These effects of the present invention were evaluated as follows. 
         [0051]    A conventional microbial agent prepared by the screening step (S1) and the culturing and mixing step (S2) was analyzed for deodorizing ability. 
         [0052]    Separately, a microbial agent prepared by the method comprising the screening step (S1) to the purification step (S4) was subjected to a deodorization test, and a safety test for fish. 
         [0053]    For deodorization testing of the microbial agents, a detection tube method was performed according to KS I2218 in the Korea Conformity Laboratories. 
         [0054]    The microbial agent prepared by the culturing (S2-1) and mixing step (S2-2) were examined for deodorizing performance against ammonia, trimethylamine, formamide and hydrogen sulfide (Example 1). 
         [0055]    The inventive microbial agent prepared by further conducting the sterilizing, step (S3) in which the microbe culture was boiled at 99° C. on a hot plate for 5 min and then cooled to room temperature, and the purifying step (S4) was also evaluated for ability to deodorize ammonia, trimethylamine, formamide, and hydrogen sulfide (Example 2). 
         [0056]    As will be elucidated hereinafter, the microbial agent was found to deodorize the odorous emissions more fast with higher efficiency when prepared by conducting the screening step (S1) to the purifying step (S4) than the screening step (S1) and the culturing (S2-1) and mixing step (S2-2) only. 
         [0057]      FIG. 3  shows fishes exposed to the microbe culture prepared following up to the sterilizing step (S3) and the purifying step (S4), demonstrating that the microbial deodorizer prepared by the method of the present invention is safe for use in living environments. 
         [0058]      FIG. 4  is a picture of a commercial product containing the microbial deodorizer of the present invention. 
         [0059]    As delineated hitherto, the present invention provides a deodorizer comprising a microbial agent. The processes of manufacturing the deodorizer are illustrated in detail in  FIG. 2 , but are not limited to those shown in the flow chart. 
         [0060]    A better understanding of the present invention may be obtained, through the following examples which are set forth to illustrate, but are not to be construed as limiting the present invention. 
       EXAMPLE 1 
       [0061]    A microbial agent prepared by conducting the screening step (S1) and the culturing (S2-1) and mixing (S2-2) step was measured for deodorizing performance against ammonia (Table 1), trimethylamine (Table 2), formaldehyde (Table 3), and hydrogen sulfide (Table 4). 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Deodorizing Effect of Microbial Agent Prepared by a Process Conducted 
               
               
                 to the Culturing and Mixing Step (S2) on Ammonia Gas 
               
             
          
           
               
                   
                 Test Result 
               
             
          
           
               
                 Test Item 
                 Blank Conc. 
                 Sample Conc. 
                 Deodorization 
               
               
                 Ammonia NH 3   
                 (μmol/mol) 
                 (μmol/mol) 
                 Rate (%) 
               
               
                   
               
             
          
           
               
                  0 min 
                 50 
                 50 
                 0.0 
               
               
                 30 min 
                 49 
                 4 
                 93.9 
               
               
                 60 min 
                 49 
                 2 
                 95.9 
               
               
                 90 min 
                 49 
                 2 
                 95.9 
               
               
                 120 min  
                 48 
                 1 
                 97.9 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Deodorizing Effect of Microbial Agent Prepared by a Process Conducted 
               
               
                 to the Culturing and Mixing Step (S2) on Triethylamine Gas 
               
             
          
           
               
                 Test Item 
                 Test Result 
               
             
          
           
               
                 Triethylamine 
                 Blank Conc. 
                 Sample Conc. 
                 Deodorization 
               
               
                 (CH 3 ) 3 N 
                 (μmol/mol) 
                 (μmol/mol) 
                 Rate (%) 
               
               
                   
               
             
          
           
               
                  0 min 
                 50 
                 50 
                 0.0 
               
               
                 30 min 
                 49 
                 5 
                 89.8 
               
               
                 60 min 
                 49 
                 4 
                 91.8 
               
               
                 90 min 
                 49 
                 4 
                 91.8 
               
               
                 120 min  
                 48 
                 3 
                 93.8 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Deodorizing Effect of Microbial Agent Prepared by a Process Conducted 
               
               
                 to the Culturing and Mixing Step (S2) on Formaldehyde Gas 
               
             
          
           
               
                 Test Item 
                 Test Result 
               
             
          
           
               
                 Formaldehyde 
                 Blank Conc. 
                 Sample Conc. 
                 Deodorization 
               
               
                 HCHO 
                 (μmol/mol) 
                 (μmol/mol) 
                 Rate (%) 
               
               
                   
               
             
          
           
               
                  0 min 
                 50 
                 50 
                 0.0 
               
               
                 30 min 
                 49 
                 10 
                 79.6 
               
               
                 60 min 
                 49 
                 10 
                 79.6 
               
               
                 90 min 
                 49 
                 10 
                 79.6 
               
               
                 120 min  
                 48 
                 9 
                 81.2 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Deodorizing Effect of Microbial Agent Prepared 
               
               
                 by a Process Conducted to the Culturing and 
               
               
                 Mixing Step (S2) on Hydrogen Sulfide Gas 
               
             
          
           
               
                 Test Item 
                 Test Result 
                   
               
             
          
           
               
                 Hydrogen 
                 Blank Conc. 
                 Sample Conc. 
                 Deodorization 
               
               
                 Sulfide H 2 S 
                 (μmol/mol) 
                 (μmol/mol) 
                 Rate (%) 
               
               
                   
               
             
          
           
               
                  0 min 
                 50 
                 50 
                 0.0 
               
               
                 30 min 
                 49 
                 39 
                 20.4 
               
               
                 60 min 
                 49 
                 38 
                 22.4 
               
               
                 90 min 
                 49 
                 38 
                 22.4 
               
               
                 120 min  
                 48 
                 37 
                 22.9 
               
               
                   
               
             
          
         
       
     
       EXAMPLE 2 
       [0062]    After the microbe culture was boiled at 99° C. on a hot plate for 5 min and cooled to room temperature in the sterilizing step (S3) and then filtered (S4) after the culturing and mixing step (S2), it was tested for ability to remove ammonia (Table 5), trimethylamine (Table 6), formaldehyde (Table 7), and hydrogen sulfide (Table 8). 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Deodorizing Effect of Microbial Agent Prepared 
               
               
                 by a Process Conducted to the Sterilizing (S3) 
               
               
                 and the Purifying Step (S4) on Ammonia Gas 
               
             
          
           
               
                   
                 Test Result 
               
             
          
           
               
                 Test Item 
                 Blank Conc. 
                 Sample Conc. 
                 Deodorization 
               
               
                 Ammonia NH 3   
                 (μmol/mol) 
                 (μmol/mol) 
                 Rate (%) 
               
               
                   
               
             
          
           
               
                  0 min 
                 50 
                 50 
                 0.0 
               
               
                 30 min 
                 49 
                 2 
                 95.9 
               
               
                 60 min 
                 49 
                 1 
                 98.0 
               
               
                 90 min 
                 49 
                 N.D 
                 100.0 
               
               
                 120 min  
                 48 
                 N.D 
                 100.0 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Deodorizing Effect of Microbial Agent Prepared by 
               
               
                 a Process Conducted to the Sterilizing (S3) and 
               
               
                 the Purifying Step (S4) on Trimethylamine Gas 
               
             
          
           
               
                 Test Item 
                 Test Result 
               
             
          
           
               
                 Trimethylamine 
                 Blank Conc. 
                 Sample Conc. 
                 Deodorization 
               
               
                 (CH 3 ) 3 N 
                 (μmol/mol) 
                 (μmol/mol) 
                 Rate (%) 
               
               
                   
               
             
          
           
               
                  0 min 
                 50 
                 50 
                 0.0 
               
               
                 30 min 
                 49 
                 2 
                 95.9 
               
               
                 60 min 
                 49 
                 1 
                 98.0 
               
               
                 90 min 
                 49 
                 N.D 
                 100.0 
               
               
                 120 min  
                 48 
                 N.D 
                 100.0 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 Deodorizing Effect of Microbial Agent Prepared 
               
               
                 by a Process Conducted to the Sterilizing (S3) 
               
               
                 and the Purifying Step (S4) on Formaldehyde Gas 
               
             
          
           
               
                 Test Item 
                 Test Result 
               
             
          
           
               
                 Formaldehyde 
                 Blank Conc. 
                 Sample Conc. 
                 Deodorization 
               
               
                 HCHO 
                 (μmol/mol) 
                 (μmol/mol) 
                 Rate (%) 
               
               
                   
               
             
          
           
               
                  0 min 
                 50 
                 50 
                 0.0 
               
               
                 30 min 
                 49 
                 7 
                 85.7 
               
               
                 60 min 
                 49 
                 6 
                 87.8 
               
               
                 90 min 
                 49 
                 6 
                 87.8 
               
               
                 120 min  
                 48 
                 5 
                 89.6 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 Deodorizing Effect of Microbial Agent Prepared by 
               
               
                 a Process Conducted to the Sterilizing (S3) and 
               
               
                 the Purifying Step (S4) on Hydrogen Sulfide Gas 
               
             
          
           
               
                 Test Item 
                 Test Result 
                   
               
             
          
           
               
                 Hydrogen 
                 Blank Conc. 
                 Sample Conc. 
                 Deodorization 
               
               
                 Sulfide H 2 S 
                 (μmol/mol) 
                 (μmol/mol) 
                 Rate (%) 
               
               
                   
               
             
          
           
               
                  0 min 
                 50 
                 50 
                 0.0 
               
               
                 30 min 
                 49 
                 30 
                 38.8 
               
               
                 60 min 
                 49 
                 22 
                 55.1 
               
               
                 90 min 
                 49 
                 18 
                 63.3 
               
               
                 120 min  
                 48 
                 17 
                 64.6 
               
               
                   
               
             
          
         
       
     
         [0063]    The test results are also depicted in  FIGS. 5 to 8 . 
         [0064]    As can be seen in  FIG. 5 , both the microbial agents which were respectively prepared by conducting the process before the third step, and up to the third step in which the microbe culture was boiled at 99° C. on a hot plate for 5 min (S3), and the purifying step (S4) exhibited 98% or more deodorization of ammonia gas. 
         [0065]    As can be seen in  FIG. 6 , the microbial agents which was prepared by conducting the process up to the third step in which the microbe culture was boiled at 99° C. on a hot plate for 5 min (S3), and the purifying step (S4) exhibited 99% or more deodorization of trimethylamine gas. 
         [0066]    As can be seen in  FIG. 7 , both the microbial agents which were respectively prepared by conducting the process before the third step, and up to the third step in which the microbe culture was boiled at 99° C. on a hot plate for 5 min (S3) and the purifying step (S4) exhibited 98% or more deodorization of ammonia gas, with an improvement of the inventive microbial agent over the conventional microbial agent by 5-6%. 
         [0067]    As can be seen in  FIG. 8 , the microbial agent which was prepared by conducting the process to the third step in which the microbe culture was boiled at 99° C. on a hot plate for 5 min (S3), and the purifying step (S4) was improved in the deodorization of ammonia gas by as great as 200%, compared to the microbial agent which was prepared by conducting the process prior to the third step. 
         [0068]    As is apparent from  FIG. 3 , the microbe culture prepared following the sterilizing step (S3) and the purifying step (S4) is safe to fish. Also the microbial deodorizer of the present invention is demonstrated as being sate in terms of heavy metal and harmful ingredients, as indicated on the test report (Table 9) flow the Korea Conformity Laboratories. 
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 Test Report on Content of Heavy Metals and Harmful Components 
               
               
                 in Microbial Deodorizer Prepared by Process Conducted to 
               
               
                 the Sterilizing Step (S3) and Purifying Step (S4) 
               
             
          
           
               
                 Test 
                 Report 
                 Test 
                   
                   
               
               
                 Item 
                 No. 
                 Method 
                 Unit 
                 Result 
               
               
                   
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Naphthalene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Acenaphthylene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Acenaphtent 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Fluorene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Phenanthrene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Anthracene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Fluoranthrene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Pyrene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 Limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Benzo (a) 
                   
                 2008 
                   
                 (Detection 
               
               
                 fluoranthene 
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Chrysene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Benzo (b) 
                   
                 2008 
                   
                 (Detection 
               
               
                 fluoranthene 
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Benzo (k) 
                   
                 2008 
                   
                 (Detection 
               
               
                 fluoranthene 
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Benzo [a] pyrene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Dibenzo [a,h] 
                   
                 2008 
                   
                 (Detection 
               
               
                 anthracene 
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Ideno [1,2,3-cd] 
                   
                 2008 
                   
                 (Detection 
               
               
                 pyrene 
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 PAHS - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Benzo [g,h,i] 
                   
                 2008 
                   
                 (Detection 
               
               
                 perylene 
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 VACs - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Benzene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 VACs - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Toluene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 VACs - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Ethylbenzene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 VACs - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Xylene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 VACs - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 1,4-dichloro- 
                   
                 2008 
                   
                 (Detection 
               
               
                 benzene 
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 VACs - 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 Styrene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 Dichloro- 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 methane 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 Chloroform 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                   
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 Carbon 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 tetrachloride 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 1,1,1-trichloro- 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 ethylene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 1,1-dichloro- 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 ethylene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 Trichloro- 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 ethylene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 Tetrachloro- 
                 CT13-33902 
                 KS M 0027: 
                 % 
                 Not detected 
               
               
                 ethylene 
                   
                 2008 
                   
                 (Detection 
               
               
                   
                   
                   
                   
                 limit 
               
               
                   
                   
                   
                   
                 0.0005) 
               
               
                 Cd 
                 CT13-33903 
                 KS M 0032: 
                 mg/kg 
                 Not detected 
               
               
                   
                   
                 2009 
                   
                 (Limit of 
               
               
                   
                   
                   
                   
                 quantification 
               
               
                   
                   
                   
                   
                 0.02) 
               
               
                 Cu 
                 CT13-33903 
                 KS M 0032: 
                 mg/kg 
                 Not detected 
               
               
                   
                   
                 2009 
                   
                 (Limit of 
               
               
                   
                   
                   
                   
                 quantification 
               
               
                   
                   
                   
                   
                 0.03) 
               
               
                 Pb 
                 CT13-33903 
                 KS M 0032: 
                 mg/kg 
                 Not detected 
               
               
                   
                   
                 2009 
                   
                 (Limit of 
               
               
                   
                   
                   
                   
                 quantification 
               
               
                   
                   
                   
                   
                 0.20) 
               
               
                 As 
                 CT13-33903 
                 KS M 0032: 
                 mg/kg 
                 Not detected 
               
               
                   
                   
                 2009 
                   
                 (Limit of 
               
               
                   
                   
                   
                   
                 quantification 
               
               
                   
                   
                   
                   
                 0.25) 
               
               
                 Hg 
                 CT13-33903 
                 KS M 0032: 
                 mg/kg 
                 Not detected 
               
               
                   
                   
                 2009 
                   
                 (Limit of 
               
               
                   
                   
                   
                   
                 quantification 
               
               
                   
                   
                   
                   
                 0.01) 
               
               
                 Cr 
                 CT13-33903 
                 KS M 0032: 
                 mg/kg 
                 Not detected 
               
               
                   
                   
                 2009 
                   
                 (Limit of 
               
               
                   
                   
                   
                   
                 quantification 
               
               
                   
                   
                   
                   
                 0.01) 
               
               
                 Zn 
                 CT13-33903 
                 KS M 0032: 
                 mg/kg 
                 Not detected 
               
               
                   
                   
                 2009 
                   
                 (Limit of 
               
               
                   
                   
                   
                   
                 quantification 
               
               
                   
                   
                   
                   
                 0.07) 
               
               
                 Ni 
                 CT13-33903 
                 KS M 0032: 
                 mg/kg 
                 Not detected 
               
               
                   
                   
                 2009 
                   
                 (Limit of 
               
               
                   
                   
                   
                   
                 quantification 
               
               
                   
                   
                   
                   
                 0.35) 
               
               
                   
               
             
          
         
       
     
         [0069]    Prepared through a culturing step for amplifying, an autotrophic microbe of interest, and a sterilizing and purifying step for removing impurities and harmful microbes other than the autotrophic microbe, as described hitherto, the microbial deodorizer of the present invention is safe to the body and the environment as well as able to remove various odorous emissions from household sources in harmony with the virtuous cycle of the ecosystem. Thus, if does not work in an artificial or chemical manner, but in a natural manner, so as to recover the nature and environment. 
         [0070]    The microbial deodorizer can be applied to various odorous sources including a bathtub, a toilet, a wardrobe, a refrigerator, a sink, a shoes cabinet, a drain, an automobile interior, a pet and its feces. Additionally, it can neutralize secondhand smoke, and odors associated with new furniture, freshly painted surfaces in offices, a sanatoriums, schools, public wash rooms, restaurants, hospitals, etc. 
         [0071]    Although the preferred embodiments of the present invention have been disclosed for illustrative purposes, those skilled in the art will appreciate that various modifications, additions and substitutions are possible, without departing from the scope and spirit of the invention as disclosed in the accompanying claims.