Abstract:
Recombinant polypeptides are disclosed that are useful for diagnosing American trypanosomiasis, or Chagas disease, a disease caused by the infectious agent  Trypanosoma cruzi . Preferably, DNA sequences encoding the recombinant proteins are placed in plasmid vectors to be expressed in an organism.

Description:
[0001]    This application claims priority from U.S. Provisional Application No. 60/430,654, filed Dec. 4, 2002, hereby incorporated by reference in its entirety. 
     
    
     
       BACKGROUND OF THE INVENTION  
         [0002]    1. Field of the Invention  
           [0003]    The present invention relates to recombinant polypeptides that are useful for diagnosing American trypanosomiasis, or Chagas disease. Chagas disease is caused by the infectious agent  Trypanosoma cruzi . More particularly, the invention relates to specific combinations of recombinant  T. cruzi  polypeptides, synthesized using genetic engineering techniques, and to constructs and processes for producing the recombinant polypeptides, and to an assay and kit for detecting  T. cruzi  infection which employs the recombinant polypeptides.  
           [0004]    2. Background  
           [0005]    Chagas disease is a zoonosis caused by the protozoan parasite,  Trypanosoma cruzi . This organism is primarily transmitted through contact with its triatomine insect vectors, but transmission by transfusion of contaminated blood and congenital transmission also are important. Historically Chagas disease has been a public health problem in all of Latin America, with the exception of the Caribbean nations. The World Health Organization estimates that 16-18 million persons are chronically infected with  T. cruzi , and that 45,000 deaths occur each year due to the illness. Infection with  T. cruzi  is life-long and specific drug treatment lacks efficacy and often causes serious side effects. Ten to thirty percent of  T. cruzi -infected persons develop chronic symptomatic Chagas disease, and the burden of disability and mortality in the endemic countries is enormous.  
           [0006]    An estimated 80,000 to 100,000  T. cruzi -infected persons now live in the United States. These immigrants pose a risk for transfusion-associated transmission of the parasite here and in other countries to which Latin Americans have emigrated. Eight such cases have been reported in the United States, Canada, and Europe, all of which occurred in immunosuppressed patients in whom acute  T. cruzi  infection was diagnosed because of the fulminant course of the illness. Most transfusions are given to immunocompetent patients in whom acute Chagas disease would be a mild illness, and thus it is reasonable to assume that many other undetected instances of transfusion-associated transmission of  T. cruzi  have occurred in the United States and other industrialized nations. The question of whether blood donated in the United States should be screened serologically for antibodies to  T. cruzi  has been considered for at least a decade by both public and private entities involved in blood banking. A panel of experts convened in early 2000 by the American Red Cross to consider this issue recommended unanimously that our blood supply be screened serologically. Implementation of such a recommendation, however, is not an option currently because no test for  T. cruzi  infection has been cleared by the FDA for screening donated blood.  
           [0007]    Diagnosis of  T. cruzi  infection presents problems. Demographic and clinical data are suggestive at best. Parasitologic tests, e.g., xenodianosis, hemoculture and PCR are insensitive. Other serologic tests are generally insensitive and lack specificity, as false positive reactions often occur with specimens from patients having infectious diseases, such as leishmaniasis, syphilis, or malaria; autoimmune diseases; and other parasitic and non-parasitic illinesses.  
           [0008]    Such conventional tests include indirect immunofluorescence (IIF), indirect hemagglutination (IHA), and complement fixation (CF) tests, as well as enzyme-linked immunosorbent assays (ELISA or EIA). Due to the lack of sensitivity and specify of the three commonly used assays, when a sample has a positive result from any, the blood must be discarded. Table I shows that in a major Brazilian blood bank (Hemocentro, Sao Paulo, Brazil), up to 3.43% of blood donations fall into this category.  
                                                 TABLE I                                   IIF   IHA   CF   % w/Results                           +   +   +   0.68%           +   −   +   0.71%           +   +   −           −   +   +           +   −   −   2.04%           −   +   −           −   −   +                    TOTAL:   3.43%                      
 
           [0009]    Commercially available ELISAs include lysate-based tests such as the Chagas Enzyme Immunoassay (EIA), available from Abbott Laboratories of Abbott Park, Ill. (the subject of FDA 510(k) Premarket Notification No. K933716, herein incorporated by reference in its entirety); the Chagas&#39; IgG ELISA, available from Meridian Bioscience, Inc. of Cincinnati, Ohio, and its predecessor, Gull Laboratories (the subject of FDA 510(k) Premarket Notification No. K911233, herein incorporated by reference in its entirety); and the Chagas&#39; kit (EIA method), available from Hemagen Diagnostics, Inc., of Waltham, Mass. (the subject of FDA 510(k) Premarket Notification No. K930272, herein incorporated by reference in its entirely). However, because these tests have less than optimal sensitivities and specificities, their use for screening donated blood would fail to detect some  T. cruzi -infected units and also would cause substantial numbers of otherwise usable units to be discarded needlessly.  
           [0010]    One of the present inventors has previously developed a radioimmune precipitation assay (RIPA), described in Kirchhoff L V, Gam A A, Gusmao R D, Goldsmith R S, Rezende J M, Rassi A. “Increased specificity of serodiagnosis of Chagas&#39; disease by detection of antibody to the 72 and 90 kDa glycoproteins of  Trypanosoma cruzi .” J Infect Dis 1987;155:561-564, herein incorporated by reference in its entirety. This test is considered the benchmark against which other tests are measured, and it is the only current option for confirmatory testing in the United States. Unfortunately, the RIPA costs $175 per assay, and at that price, screening the approximately 13 million units of blood donated each year would cost over $2 billion.  
           [0011]    Therefore, the present inventors have further developed recombinant assays for detection of  T. cruzi  infection. A typical recombinant polypeptide and method for assaying is described by them in U.S. Pat. No. 5,876,734, No. 6,228,601, and PCT Publication No. WO 95/25797, each of which is herein incorporated by reference in its entirety. Such assays for  T. cruzi  infection based on recombinant antigens, in contrast to those utilizing native antigens (e.g., the conventional lysate-based assays), as discussed above, will be more accurate, i.e., the sensitivity and specificity will be higher.  
           [0012]    Furthermore, the recombinant assays of the invention present manufacturing advantages over the materials for the RIPA and conventional tests. Once the molecular biology has been completed, the recombinant antigens are produced in  Escherichia coli , thus eliminating completely any biohazard associated with growing the parasites in liquid culture. This is a substantive advantage, as many cases of laboratory-acquired  T. cruzi  infection have been reported. Additionally, recombinant antigens produced in  E. coli  are much easier to purify, quantitate, and standardize than antigen lysates produced in liquid cultures of parasites, thus facilitating the manufacture of a consistent product and simplifying compliance with governmental regulations. A final advantage lies in the fact that several of the recombinant proteins presented in this application are comprised of two to four distinct protein segments derived from separate  T. cruzi  genes. This use of hybrid recombinant proteins also facilitates manufacture of an assay in that several antigenically distinct proteins are obtained in a single purification, quantitation, and standardization run.  
         SUMMARY OF THE INVENTION  
         [0013]    The present invention utilizes recombinant proteins for detecting  T. cruzi  infected blood. The invention utilizes specific polypeptide sequences that correspond to fusion proteins FP3, FP4, FP5, FP6, FP7, FP8, FP9 and FP10 as described below. Isolated polynucleotides that encode the inventive polypeptides according to the present invention are also utilized, as are cells transformed with a recombinant plasmid that expresses a polypeptide according to the invention. The present invention is similar to that which is described in U.S. Pat. No. 5,876,734, herein incorporated by reference in its entirety. However, the present invention replaces the proteins in the process with the recombinant proteins of this invention to achieve similar or superior results.  
           [0014]    The present invention also provides a method for detecting the presence of antibodies to  T. cruzi  in an individual, comprising the steps of contacting a putative anti- T. cruzi  antibody-containing sample from an individual with a polypeptide according to the invention that is typically attached or conjugated to a carrier molecule or attached or conjugated to a solid phase; allowing anti- T. cruzi  and other antibodies in said sample to bind to said polypeptide; washing away unbound anti- T. cruzi  antibodies; and adding a compound that enables detection of the anti- T. cruzi  antibodies which are specifically bound to the polypeptide. The compound that enables detection of the anti- T. cruzi  antibodies may be selected from the group consisting of a colorometric agent, a fluorescent agent, a chemiluminescent agent and a radionucleotide.  
           [0015]    Also provided in accordance with the present invention is a kit for diagnosing the presence of anti- T. cruzi  antibodies in a sample, comprising a container in which a polypeptide according to the invention is attached or conjugated to a carrier molecule or attached or conjugated to a solid phase; and directions for carrying out the method according to the invention. The kit additionally may comprise a container of a compound that binds to anti- T. cruzi  antibodies and that renders said antibodies detectable.  
           [0016]    Other objects, features and advantages of the present invention will become apparent from the following detailed description. It should be understood, however, that the detailed description and the specific examples, while indicating preferred embodiments of the invention, are given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description. 
       
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0017]    [0017]FIG. 1 is a description of the prior art.  
         [0018]    [0018]FIGS. 1 a - 1   h  are schematic representations of the recombinant proteins utilized in the invention.  
         [0019]    [0019]FIG. 2 is a bar graph showing reactivity of various blood specimens with recombinant proteins used alone or in combination as target antigens in ELISAs. 
     
    
     DETAILED DESCRIPTION OF THE INVENTION  
       [0020]    [0020]FIGS. 1 a - 1   h  represent the recombinant proteins of the invention, with the various letters indicating known protein sequences, as follows. The Figs. are schematic diagrams of the recombinant  T. cruzi  proteins, comprised of segments A through L. Solid segments (A, C, D, F, H, I, and K) represent nonrepetitive proteins having amino acid sequences that are unrelated to each other. Saw-tooth segments (B, E, G, J, and L) represent repetitive proteins having amino acid sequences that are unrelated to each other and unrelated to those of the nonrepetitive proteins. The relative sizes and numbers of repeats in the repetitive proteins are roughly represented in the Figs. The sizes and shapes of the nonrepetitive segments bear no relation to the actual proteins.  
         [0021]    The following information refers to FIGS. 1 and 1 a - 1   h  in which the recombinant proteins Ag15, FP3, FP4, FP5, FP6, FP7, FP8, FP9 and FP10 are depicted schematically. These proteins are derived from  T. cruzi , the protozoan parasite that causes Chagas disease, and are formed from of proteins A through L as indicated, and defined herein. There are no substantive amino acid similarities among proteins A through L. Similarly there are no substantive DNA sequence similarities among the segments that encode proteins A through L. The  T. cruzi  DNA sequences that encode proteins A through L were cloned in combination into pGEX and pET plasmid vectors, such as pET-32a. Strains of  Escherichia coli  were transfected with the recombinant vectors bearing the  T. cruzi  DNA sequences, and the bacteria were incubated in liquid culture under conditions favoring synthesis of the recombinant proteins. The latter proteins were subsequently affinity-purified and then used as target antigens in ELISAs. ELISAs in which proteins Ag15, FP3, FP4, FP5, FP6, FP7, FP8, FP9, and FP10, alone or in combination are employed as target antigens are useful as sensitive and specific detectors of anti- T. cruzi  antibodies in blood specimens obtained from persons who are chronically infected with this parasite. The detection of such antibodies is the primary means of identifying persons who are chronically infected with  T. cruzi.    
         [0022]    The following paragraphs contain information relating to the naming, localization, and function of proteins A through L, as well as the corresponding GenBank accession numbers of the sequences to which they are related and relevant publications.  
         [0023]    It should be noted that the  T. cruzi  gene segments that encode protein segments A through L generally are shortened versions of the native coding regions. In this context, the constructs that encode single segments (i.e., FP5 and FP9), as well as all the others that encode more than one segment, are all unique, because, even if the individual components from which the various recombinant proteins of this invention are known, the segments of the invention have not been combined previously as described herein.  
         [0024]    Protein AB. This hybrid recombinant protein, also designated Ag15 [SEQ ID NO. 2] in FIG. 1, is derived from the TCR27 gene of  T. cruzi  [SEQ ID NO. 1]. Protein A is the amino terminal nonrepetitive portion of the TCR27 protein, and Protein B is comprised of approximately 18 of the 14 amino acid repeats that make up the central portion of the TCR27 protein. The two native TCR27 genes sequenced contained approximately 69 and 105 of the 14-amino acid repeats.  
         [0025]    Nucleotide sequence data that include the Ag15 DNA sequence were deposited with GenBank and EMBL databases by Keiko Otsu, John E. Donelson, and Louis V. Kirchhoff with the accession number L04603 and are described in U.S. Pat. No. 5,876,734 and No. 6,228,601, issued to Louis V. Kirchhoff and Keiko Otsu (each of which is herein incorporated by reference in its entirety). These references also present DNA and inferred protein sequences that include the Ag15 DNA and inferred protein sequences. The Ag15 DNA and inferred protein sequences are additionally presented in Otsu K, Donelson J E, Kirchhoff L V. “Interruption of a  Trypanosoma cruzi  gene encoding a protein containing 14-amino acid repeats by targeted insertion of the neomycin phosphotransferase gene.” Mol Biochem Parasitol 1993;57:317-330, herein incorporated by reference in its entirety.  
         [0026]    Protein C. This is a calcium binding protein of  T. cruzi , initially called 1F8 and later designated the flagellar calcium binding protein (FCaBP) [SEQ ID NO 4]. The accession number of the original 1 F8 DNA sequence [SEQ ID NO 3] deposited in GenBank is K03278. The Protein C DNA and inferred protein sequences are presented in Gonzalez A, Lemer T J, Huecas M, Sosa-Pineda B, Nogueira N, Lizardi P M. “Apparent generation of a segmented mRNA from two separate tandem gene families in  Trypanosoma cruzi .” Nucleic Acids Res 1985;13(16):5789-804, herein incorporated by reference in its entirety.  
         [0027]    [0027]FIG. 1 a  shows a first protein (FP3) [SEQ ID NO. 22] in accordance with the invention. Specifically, FP3 corresponds essentially to the combination of Ag15 (FIG. 1), and by Protein C. The DNA sequence encoding FP3 [SEQ ID NO 21], also essentially corresponds to the sequences coding for Ag15 and Protein C.  
         [0028]    Protein D. This is the protein core of a surface glycoprotein of  T. cruzi  that is referred to as GP72 [SEQ ID NO 6]. The accession number of the original gp72 DNA sequence [SEQ ID NO 5] deposited in GenBank is M65021. The Protein D DNA and inferred protein sequences are presented in Cooper R, Inverso J A, Espinosa M, Nogueira N, Cross G A. “Characterization of a candidate gene for GP72, an insect stage-specific antigen of  Trypanosoma cruzi .” Mol Biochem Parasitol 1991;49(1):45-59, herein incorporated by reference in its entirety.  
         [0029]    [0029]FIG. 1 b  shows a second protein (FP4) [SEQ ID NO 8] in accordance with the invention. The DNA sequence [SEQ ID NO 7] that encodes Protein DABC which is a single continuous coding region, essentially corresponds to the DNA sequences from which it was constructed.  
         [0030]    Protein E. This is a segment of the flagellar repetitive protein (FRA) [SEQ ID NO 10] of  T. cruzi  comprised of approximately nine repeats consisting of 68 amino acids each, shown as FIG. 1 c  (FP5). The accession number of the original Protein E DNA sequence [SEQ ID NO 9] deposited in GenBank is J04015. The Protein E DNA and inferred protein sequences are presented in Lafaille J J, Linss J, Krieger M A, Souto-Padron T, de Souza W, Goldenberg S. “Structure and expression of two  Trypanosoma cruzi  genes encoding antigenic proteins bearing repetitive epitopes.” Mol Biochem Parasitol 1989; 35(2): 127-136, herein incorporated by reference in its entirety.  
         [0031]    Protein FGH. This is a protein [SEQ ID NO 12] encoded by a modified version of the  T. cruzi  TCR39 gene that was artificially constructed [SEQ ID NO 11], shown as FIG. 1 e  (FP7). The modification entailed reducing the length of the central portion of the TCR39 gene that encodes the 12-amino acid repeats. Protein F is the amino terminal nonrepetitive segment of the TCR39 protein. Protein G is comprised of approximately 13 of the 12-amino acid repeats that make up the central portion of the TCR39 protein. Protein H is the carboxy terminal nonrepetitive segment of the TCR39 protein. The accession number of the original, i.e., the unmodified, Protein FGH DNA sequence deposited in GenBank is U15616. The TCR39 DNA and inferred protein sequences, which include the entire Protein FGH sequences, are presented in Gruber A, Zingales B. “ Trypanosoma cruzi : characterization of two recombinant antigens with potential application in the diagnosis of Chagas&#39; disease.” Exp Parasitol 1993;76(1):1-12, herein incorporated by reference in its entirety.  
         [0032]    [0032]FIG. 1 d  shows another hybrid recombinant protein (FP6, Protein FGHE) [SEQ ID NO 14] in accordance with the invention. The DNA sequence that encodes Protein FGHE [SEQ ID NO 13], which is a single continuous coding region, essentially corresponds to the DNA sequences from which it was constructed.  
         [0033]    Protein IJK. This is a protein [SEQ ID NO 16] encoded by a modified version of the  T. cruzi  shed acute phase antigen (SAPA) gene that was artificially constructed [SEQ ID NO 15], as shown in FIG. 1 f  (FP8). The modification entailed reducing the length of the central portion of the SAPA gene that consists of 12-amino acid repeats. Protein I is the amino terminal nonrepetitive segment of the SAPA protein. Protein J is comprised of approximately nine of the 12-amino acid repeats that make up the central portion of the SAPA protein. Protein K is the carboxy terminal nonrepetitive segment of the SAPA protein. The accession number of the original, i.e., the unmodified, Protein IJK DNA sequence deposited in Gen Bank is J03985. The SAPA DNA and protein sequences, which include the entire Protein IJK sequences, are presented in Affranchino J L, Pollevick G D, Frasch A C C. “The expression of the major shed  Trypanosoma cruzi  antigen results from the developmentally-regulated transcription of a small gene family.” FEBS Lett 1991;280:316-320, herein incorporated by reference in its entirety.  
         [0034]    Protein L. This is a microtubule-associated repetitive protein (MAP) [SEQ ID NO 18] of  T. cruzi  that is comprised of approximately five repeats consisting of 38 amino acids each, as depicted in FIG. 1 g  (FP9). The accession number of the original Protein L DNA sequence [SEQ ID NO 17] deposited in GenBank is S68286. The Protein L DNA and inferred protein sequences are presented in Kemer N, Liegeard P, Levin M J, Hontebeyrie-Joskowicz M. “ Trypanosoma cruzi : antibodies to a MAP-like protein in chronic Chagas&#39; disease cross-react with mammalian cytoskeleton.” Experimental Parasitology 1991;73(4):451-459, herein incorporated by reference in its entirety.  
         [0035]    [0035]FIG. 1 h  shows another hybrid recombinant protein (FP10, Protein IJKL) [SEQ ID NO 20] in accordance with the invention. The DNA sequence that encodes Protein IJKL [SEQ ID NO 19], which is a single continuous coding region, essentially corresponds to the DNA sequences from which it was constructed.  
         [0036]    Additionally, combinations of the various recombinant proteins depicted in the Figs. may be used. While it is possible to combine one or more of the recombinant proteins to form longer recombinant proteins, typically more than one recombinant protein is used simultaneously. For example, simultaneous uses of FP4 and FP5, FP5 and FP6, as well as FP4 and FP6, and combinations using more than two recombinant proteins (e.g., FP4, FP6 and FP10) are considered within the scope of the present invention. It is believed that the sensitivity and specificity of the assays according to the invention are sufficient to meet FDA standards for screening the blood supply of the United States.  
         [0037]    Additionally, as described in U.S. Pat. No. 6,228,601 (herein incorporated by reference in its entirety), polypeptides need not correspond exactly over their entire lengths to be considered within the scope of the invention. For example, a wide variety of polypeptides which contain at least one epitope embodied in the polypeptides of the invention can be used in accordance with the present invention. Based on the nucleotide sequences, polypeptide molecules also can be produced (1) that include sequence variations, relative to the naturally-occurring sequences, (2) that have one or more amino acids truncated from the naturally-occurring sequences and variations thereof, or (3) that contain the naturally-occurring sequences and variations thereof as part of a longer sequence.  
         [0038]    In this description, polypeptide molecules in categories (1), (2) and (3) are said to “correspond” to the amino acid sequences of the recombinant proteins of the invention. Such polypeptides also are referred to as “variants.” The category of variants within the present invention includes, for example, fragments and muteins of proteins A though L, as well as larger molecules that consist essentially at least one protein sequence A through L, alone or in combination with other proteins A to L.  
         [0039]    In this regard, a molecule that “consists essentially of” protein A to L, alone or in combination with any other proteins A to L, is one that is immunoreactive with samples from persons infected with  T. cruzi , but that does not react with samples from patients with leishmaniasis, schistosomiasis, and other parasitic and infectious diseases, with samples from patients with autoimmune disorders and other illnesses, and with specimens from normal persons.  
         [0040]    A “mutein” is a polypeptide that is homologous to the protein to which it corresponds, and that retains the basic functional attribute—the ability to react selectively with samples from persons infected with  T. cruzi  —of the corresponding region. For purposes of this description, “homology” between two sequences connotes a likeness short of identity indicative of a derivation of the first sequence from the second. In particular, a polypeptide is “homologous” to the corresponding protein if a comparison of amino acid sequences between the polypeptide and the corresponding region reveals an identity of greater than 40%, preferably greater than 50% and more preferably 70%. Such sequence comparisons can be performed via known algorithms, such as those described in Pearson W R, Lipman D J. “Improved tools for biological sequence comparison.” Proc Natl Acad Sci USA 1988;85(8):2444-2448, herein incorporated by reference in its entirety, which are readily implemented by computer.  
         [0041]    A fragment of a protein of the invention is a molecule in which one or more amino acids are truncated from that protein. Muteins and fragments can be produced, in accordance with the present invention, by known de novo synthesis techniques.  
         [0042]    Also exemplary of variants within the present invention are molecules that are longer than a protein of the invention, but that contain the region or a mutein thereof within the longer sequence. For example, a variant may include a futher fusion partner in addition to the protein of the invention. Such a fusion partner may allow easier purification of recombinantly-produced polypeptides. For example, use of a glutathione-S-transferase (26 kilodaltons, GST) fusion partner allows purification of recombinant polypeptides on glutathione agarose beads.  
         [0043]    The portion of the sequence of a such molecule other than that portion of the sequence corresponding to the region may or may not be homologous to the sequence of a protein of the invention.  
         [0044]    It will be appreciated that polypeptides shorter than the corresponding protein of the invention but that retain the ability to react selectively with samples from persons infected with  T. cruzi  are suitable for use in the present invention. Thus, variants may be of the same length, longer than or shorter than the protein of the invention, and also include sequences in which there are amino acid substitutions of the parent sequence. These variants must retain the ability to react selectively with samples from persons infected with  T. cruzi.    
         [0045]    In one embodiment, the assay of the invention uses FP4 as target antigen. Table II compares the results obtained by testing 45 pre-screened Argentinean specimens in an  
                                                               TABLE II                                       RIPA                    +   −                            FP4 ELISA   +   9   0               −   0   36                      
 
         [0046]    FP4 ELISA with those obtained by RIPA testing.  
         [0047]    The data in Table II show that in this group of specimens, the sensitivity and specificity of the FP4 ELISA were both 100%  
         [0048]    Similarly, the performance of an FP4+FP6 ELISA in comparison to RIPA was  
                                                               TABLE III                                       RIPA                    +   −                            FP4 + FP6 ELISA   +   10   1               −   0   78                      
 
         [0049]    assessed by testing 89 pre-selected Guatemalan specimens.  
         [0050]    The data shown in Table III indicate that in this group of samples, the sensitivity of the FP4+FP6 ELISA was 100% and the specificity was 98.7%.  
         [0051]    As shown in FIG. 2, in a FP4+FP6 ELISA, performed using standard procedures, a group of previously characterized RIPA-positive samples from several Chagas-endemic countries gave a mean reactivity (absorbance) of 2.99. Thus FP4+FP6 is the preferred embodiment among the recombinant proteins tested alone and in combination in that experiment.  
         [0052]    It should be apparent that embodiments other than those specifically described above may come within the spirit and scope of the present invention, such as recombinant proteins comprised of different combinations and/or spatial arrangements of proteins A to L. Hence, the present invention is not limited by the above description.  
     
       
       
         1 
         
           
             50  
           
           
             1  
             1521  
             DNA  
             T. CRUZI  
             
               CDS  
               (1)..(1521)  
             
           
            1 

tat ggc ccg agc tgt ggt gct tga gga tgg agc gct tta cgt ggc gga       48 
Tyr Gly Pro Ser Cys Gly Ala     Gly Trp Ser Ala Leu Arg Gly Gly 
1               5                       10                  15 

caa tgc caa caa cct cgt tcg aga aat ctc caa tgg cgt tgt cac ttc       96 
Gln Cys Gln Gln Pro Arg Ser Arg Asn Leu Gln Trp Arg Cys His Phe 
                20                  25                  30 

gtt tat tac gga agg act gct ggg ccc atc gta cat caa acc gta cag      144 
Val Tyr Tyr Gly Arg Thr Ala Gly Pro Ile Val His Gln Thr Val Gln 
            35                  40                  45 

ccg tac aaa tgg cgc tca tga ctt gtt tgt gtc gga cac ggg caa atc      192 
Pro Tyr Lys Trp Arg Ser     Leu Val Cys Val Gly His Gly Gln Ile 
        50                      55                  60 

acg cat cat ttt tgc ccc acc tca gaa aaa aac gtt cat cac agt gtt      240 
Thr His His Phe Cys Pro Thr Ser Glu Lys Asn Val His His Ser Val 
        65                  70                  75 

tat aac agg att cca gcc gga tgt tct tca aat tag cga gaa gag tcg      288 
Tyr Asn Arg Ile Pro Ala Gly Cys Ser Ser Asn     Arg Glu Glu Ser 
    80                  85                      90 

ttt gat gtt tgc cat ctg caa ttc cac gaa aat tct tgc gat taa tat      336 
Phe Asp Val Cys His Leu Gln Phe His Glu Asn Ser Cys Asp     Tyr 
    95                  100                 105 

gca ggg agc cac aac ccc gaa gga gta ctg gca agt tgg aaa tgc gga      384 
Ala Gly Ser His Asn Pro Glu Gly Val Leu Ala Ser Trp Lys Cys Gly 
    110                 115                 120 

ctg cat ggg cta tca gag ttc cct cat gct cac gac cga gga gga taa      432 
Leu His Gly Leu Ser Glu Phe Pro His Ala His Asp Arg Gly Gly 
125                 130                 135 

act cct cta cta cgg cat att aaa tgg aac ccc atc cat cat gtc ttt      480 
Thr Pro Leu Leu Arg His Ile Lys Trp Asn Pro Ile His His Val Phe 
140                 145                 150                 155 

acc cgc cac caa aac gaa gac gga agc acc cag aat ttg ccc gga tgt      528 
Thr Arg His Gln Asn Glu Asp Gly Ser Thr Gln Asn Leu Pro Gly Cys 
                160                 165                 170 

gtt gtt gca gtg gcc aca tgg gcc cat tgt ttc gct tgt gaa tat taa      576 
Val Val Ala Val Ala Thr Trp Ala His Cys Phe Ala Cys Glu Tyr 
            175                 180                 185 

caa aca tgc att tta cgt tgt tac cgc ctc caa tgt ata cat tgt aca      624 
Gln Thr Cys Ile Leu Arg Cys Tyr Arg Leu Gln Cys Ile His Cys Thr 
            190                 195                 200 

tga tgg ctc gta tca tcc gac tgg atc cat ggc cca gct cca aca ggc      672 
    Trp Leu Val Ser Ser Asp Trp Ile His Gly Pro Ala Pro Thr Gly 
            205                 210                 215 

aga aaa taa tat cac taa ttc caa aaa aga aat gac aaa gct acg aga      720 
Arg Lys     Tyr His     Phe Gln Lys Arg Asn Asp Lys Ala Thr Arg 
            220                     225                 230 

aaa agt gaa aaa ggc cga gaa aga aaa att gga cgc cat taa ccg ggc      768 
Lys Ser Glu Lys Gly Arg Glu Arg Lys Ile Gly Arg His     Pro Gly 
            235                 240                     245 

aac caa gct gga aga gga acg aaa cca agc gta caa agc agc aca caa      816 
Asn Gln Ala Gly Arg Gly Thr Lys Pro Ser Val Gln Ser Ser Thr Gln 
            250                 255                 260 

ggc aga gga gga aaa ggc taa aac att tca acg cct tat aac att tga      864 
Gly Arg Gly Gly Lys Gly     Asn Ile Ser Thr Pro Tyr Asn Ile 
        265                     270                 275 

gtc gga aaa tat taa ctt aaa gaa aag gcc aaa tga cgc agt ttc aaa      912 
Val Gly Lys Tyr     Leu Lys Glu Lys Ala Lys     Arg Ser Phe Lys 
            280                     285                     290 

tcg gga taa gaa aaa aaa ttc tga aac cgc aaa aac tga cga agt aga      960 
Ser Gly     Glu Lys Lys Phe     Asn Arg Lys Asn     Arg Ser Arg 
                    295                     300 

gaa aca gag ggc ggc tga ggc tgc caa ggc cgt gga gac gga gaa gca     1008 
Glu Thr Glu Gly Gly     Gly Cys Gln Gly Arg Gly Asp Gly Glu Ala 
    305                     310                 315 

gag ggc agc tga ggc cac gaa ggt tgc cga agc gga gaa gcg gaa ggc     1056 
Glu Gly Ser     Gly His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly 
    320                     325                 330 

agc tga ggc cgc caa ggc cgt gga gac gga gaa gca gag ggc agc tga     1104 
Ser     Gly Arg Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser 
        335                 340                 345 

agc cac gaa ggt tgc cga agc gga gaa gca gaa ggc agc tga ggc cgc     1152 
Ser His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly Ser     Gly Arg 
        350                 355                 360 

caa ggc cgt gga gac gga gaa gca gag ggc agc tga agc cac gaa ggt     1200 
Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser     Ser His Glu Gly 
        365                 370                     375 

tgc cga agc gga gaa gca gag ggc agc tga agc cat gaa ggt tgc cga     1248 
Cys Arg Ser Gly Glu Ala Glu Gly Ser     Ser His Glu Gly Cys Arg 
        380                 385                     390 

agc gga gaa gca gaa ggc agc tga ggc cgc caa ggc cgt gga gac gga     1296 
Ser Gly Glu Ala Glu Gly Ser     Gly Arg Gln Gly Arg Gly Asp Gly 
        395                     400                 405 

gaa gca gag ggc agc tga agc cac gaa ggt tgc cga agc gga gaa gca     1344 
Glu Ala Glu Gly Ser     Ser His Glu Gly Cys Arg Ser Gly Glu Ala 
        410                     415                 420 

gaa ggc agc tga ggc cgc caa ggc cgt gga gac gga gaa gca gag ggc     1392 
Glu Gly Ser     Gly Arg Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly 
        425                     430                 435 

agc tga agc cac gaa ggt tgc cga agc gga gaa gca gaa ggc agc tga     1440 
Ser     Ser His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly Ser 
            440                 445                 450 

ggc cgc caa ggc cgt gga gac gga gaa gca gag ggc agc tga agc cac     1488 
Gly Arg Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser     Ser His 
            455                 460                     465 

gaa ggt tgc cga agc gga gaa gga tat cga tcc                         1521 
Glu Gly Cys Arg Ser Gly Glu Gly Tyr Arg Ser 
            470                 475 

 
           
             2  
             7  
             PRT  
             T. CRUZI  
           
            2 

Tyr Gly Pro Ser Cys Gly Ala 
1               5 

 
           
             3  
             46  
             PRT  
             T. CRUZI  
           
            3 

Gly Trp Ser Ala Leu Arg Gly Gly Gln Cys Gln Gln Pro Arg Ser Arg 
1               5                   10                  15 

Asn Leu Gln Trp Arg Cys His Phe Val Tyr Tyr Gly Arg Thr Ala Gly 
            20                  25                  30 

Pro Ile Val His Gln Thr Val Gln Pro Tyr Lys Trp Arg Ser 
        35                  40                  45 

 
           
             4  
             36  
             PRT  
             T. CRUZI  
           
            4 

Leu Val Cys Val Gly His Gly Gln Ile Thr His His Phe Cys Pro Thr 
1               5                   10                  15 

Ser Glu Lys Asn Val His His Ser Val Tyr Asn Arg Ile Pro Ala Gly 
            20                  25                  30 

Cys Ser Ser Asn 
        35 

 
           
             5  
             18  
             PRT  
             T. CRUZI  
           
            5 

Arg Glu Glu Ser Phe Asp Val Cys His Leu Gln Phe His Glu Asn Ser 
1               5                   10                  15 

Cys Asp 

 
           
             6  
             32  
             PRT  
             T. CRUZI  
           
            6 

Tyr Ala Gly Ser His Asn Pro Glu Gly Val Leu Ala Ser Trp Lys Cys 
1               5                   10                  15 

Gly Leu His Gly Leu Ser Glu Phe Pro His Ala His Asp Arg Gly Gly 
            20                  25                  30 

 
           
             7  
             47  
             PRT  
             T. CRUZI  
           
            7 

Thr Pro Leu Leu Arg His Ile Lys Trp Asn Pro Ile His His Val Phe 
1               5                   10                  15 

Thr Arg His Gln Asn Glu Asp Gly Ser Thr Gln Asn Leu Pro Gly Cys 
            20                  25                  30 

Val Val Ala Val Ala Thr Trp Ala His Cys Phe Ala Cys Glu Tyr 
        35                  40                  45 

 
           
             8  
             16  
             PRT  
             T. CRUZI  
           
            8 

Gln Thr Cys Ile Leu Arg Cys Tyr Arg Leu Gln Cys Ile His Cys Thr 
1               5                   10                  15 

 
           
             9  
             17  
             PRT  
             T. CRUZI  
           
            9 

Trp Leu Val Ser Ser Asp Trp Ile His Gly Pro Ala Pro Thr Gly Arg 
1               5                   10                  15 

Lys 

 
           
             10  
             23  
             PRT  
             T. CRUZI  
           
            10 

Phe Gln Lys Arg Asn Asp Lys Ala Thr Arg Lys Ser Glu Lys Gly Arg 
1               5                   10                  15 

Glu Arg Lys Ile Gly Arg His 
            20 

 
           
             11  
             24  
             PRT  
             T. CRUZI  
           
            11 

Pro Gly Asn Gln Ala Gly Arg Gly Thr Lys Pro Ser Val Gln Ser Ser 
1               5                   10                  15 

Thr Gln Gly Arg Gly Gly Lys Gly 
            20 

 
           
             12  
             8  
             PRT  
             T. CRUZI  
           
            12 

Asn Ile Ser Thr Pro Tyr Asn Ile 
1               5 

 
           
             13  
             4  
             PRT  
             T. CRUZI  
           
            13 

Val Gly Lys Tyr 
1 

 
           
             14  
             6  
             PRT  
             T. CRUZI  
           
            14 

Leu Lys Glu Lys Ala Lys 
1               5 

 
           
             15  
             6  
             PRT  
             T. CRUZI  
           
            15 

Arg Ser Phe Lys Ser Gly 
1               5 

 
           
             16  
             4  
             PRT  
             T. CRUZI  
           
            16 

Glu Lys Lys Phe 
1 

 
           
             17  
             4  
             PRT  
             T. CRUZI  
           
            17 

Asn Arg Lys Asn 
1 

 
           
             18  
             8  
             PRT  
             T. CRUZI  
           
            18 

Arg Ser Arg Glu Thr Glu Gly Gly 
1               5 

 
           
             19  
             13  
             PRT  
             T. CRUZI  
           
            19 

Gly Cys Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             20  
             13  
             PRT  
             T. CRUZI  
           
            20 

Gly His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             21  
             13  
             PRT  
             T. CRUZI  
           
            21 

Gly Arg Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             22  
             13  
             PRT  
             T. CRUZI  
           
            22 

Ser His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             23  
             13  
             PRT  
             T. CRUZI  
           
            23 

Gly Arg Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             24  
             13  
             PRT  
             T. CRUZI  
           
            24 

Ser His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             25  
             13  
             PRT  
             T. CRUZI  
           
            25 

Ser His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             26  
             13  
             PRT  
             T. CRUZI  
           
            26 

Gly Arg Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             27  
             13  
             PRT  
             T. CRUZI  
           
            27 

Ser His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             28  
             13  
             PRT  
             T. CRUZI  
           
            28 

Gly Arg Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             29  
             13  
             PRT  
             T. CRUZI  
           
            29 

Ser His Glu Gly Cys Arg Ser Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             30  
             13  
             PRT  
             T. CRUZI  
           
            30 

Gly Arg Gln Gly Arg Gly Asp Gly Glu Ala Glu Gly Ser 
1               5                   10 

 
           
             31  
             13  
             PRT  
             T. CRUZI  
           
            31 

Ser His Glu Gly Cys Arg Ser Gly Glu Gly Tyr Arg Ser 
1               5                   10 

 
           
             32  
             42  
             DNA  
             T CRUZI  
             
               misc_feature  
               (1)..(4)  
               n is a, c, g, or t  
             
           
            32 

nnnnctatta ttgatacagt ttctgtacta tattggttgt gc                        42 

 
           
             33  
             3749  
             DNA  
             T CRUZI  
             
               CDS  
               (833)..(2575)  
             
             
               sig_peptide  
               (833)..(937)  
             
             
               mat_peptide  
               (938)..(2575)  
             
           
            33 

ccccctcgag gtcgacctgc aggtcaacgg atcttacctg agtacaaaag gtcaagtgag     60 

cggtcaaaag gatgtatata tacatatata accataaggg aaacatttgg gcatttaact    120 

gcctttacat ttcccttttc cttcaatatc ttgtttgttt gtttttggtt tctataggaa    180 

attttaggat ccggccagcg gcataggaga ttattctctt ttttattaat tgcttaatgc    240 

gttggtctgt gtgtgtgttg gttcccttgt gcgagctcac ggggcctaat tatgattgtt    300 

gcgcatatgc atatatatat atatatatat acatgtgtgt gtgtgtgtat atgtacgttt    360 

gttggtttgc cgctgtactc ccgcctgcgt gtgtctgtct ctctctctgt gtgtgtgatg    420 

ggctgcttct ctttcttttg ttgcgtccct ttattattat tatttttttt tcttctctcc    480 

cacttctctc cccgtgtggt gcacgcacag taaagataga gggagaaata gagcgagtgt    540 

ttgtatcagt gtctccgttg cggctggtac tggtagaagg agaagaatag aagaaggaga    600 

aaaaaaaaaa aaaaaaaaaa aaaagagaga gagagagaga agggcgaacg agaaaaaaga    660 

agaagaaaca tttgagaagg aattggaacg aaaattgtaa gaggaagcaa aaaaaaaaaa    720 

aaaaagtgtg tgtgtgtgag agagagagag agaggaagcc aataataata aaaagcaaac    780 

aaaaaagcaa aaacaaaaat atttgtagac cggacgtccc gtcttggacg tg atg ttt    838 
                                                           Met Phe 
                                                          -35 

tca aaa agg acg tcg cca gca ccc ttc cgt gcg ctc ctg ctg ccg gtc      886 
Ser Lys Arg Thr Ser Pro Ala Pro Phe Arg Ala Leu Leu Leu Pro Val 
            -30                 -25                 -20 

gtg gtg gtg gtg gtg gtg gtg gtg gca tct gtg gcc ctc cct gca gga      934 
Val Val Val Val Val Val Val Val Ala Ser Val Ala Leu Pro Ala Gly 
        -15                 -10                 -5 

gcg cag ttt gat tta agg cag cag cag ctg gtt ata cag gat ttc ttc      982 
Ala Gln Phe Asp Leu Arg Gln Gln Gln Leu Val Ile Gln Asp Phe Phe 
-1  1               5                   10                  15 

atc agt cgc tcc tgc gca gga tgt tca cag ggg caa acc gat ggc cca     1030 
Ile Ser Arg Ser Cys Ala Gly Cys Ser Gln Gly Gln Thr Asp Gly Pro 
                20                  25                  30 

agc ggt gcc ggc aca ctc ttc act gcc gcc ggt ggt tcg ctt ggc aaa     1078 
Ser Gly Ala Gly Thr Leu Phe Thr Ala Ala Gly Gly Ser Leu Gly Lys 
            35                  40                  45 

gat gct tcc acg ctg ctg ttg tgt gac caa ggt ggt ggt ggc tcc agc     1126 
Asp Ala Ser Thr Leu Leu Leu Cys Asp Gln Gly Gly Gly Gly Ser Ser 
        50                  55                  60 

gtg cgt ttg gtg aac aaa tcc ggc att ttc acc ctt gcc ggt agt aaa     1174 
Val Arg Leu Val Asn Lys Ser Gly Ile Phe Thr Leu Ala Gly Ser Lys 
    65                  70                  75 

acg acg cgt ggc aat caa aat ggt ccg gcg gcg acg gca ctc ttc aac     1222 
Thr Thr Arg Gly Asn Gln Asn Gly Pro Ala Ala Thr Ala Leu Phe Asn 
80                  85                  90                  95 

atg ccc cga gct gtg gtg ctt gag gat gga gcg ctt tac gtg gcg gac     1270 
Met Pro Arg Ala Val Val Leu Glu Asp Gly Ala Leu Tyr Val Ala Asp 
                100                 105                 110 

agt gcc aac aac ctc gtt cga gaa atc tcc aat ggc att gtc act tcg     1318 
Ser Ala Asn Asn Leu Val Arg Glu Ile Ser Asn Gly Ile Val Thr Ser 
            115                 120                 125 

ttt att acg gag gga ctg ctg ggc cca tcg tac atc aaa ccg tac agc     1366 
Phe Ile Thr Glu Gly Leu Leu Gly Pro Ser Tyr Ile Lys Pro Tyr Ser 
        130                 135                 140 

cgt cca aat ggc gcc cat gac ttg ttt gtg tcg gac acg ggc aaa tct     1414 
Arg Pro Asn Gly Ala His Asp Leu Phe Val Ser Asp Thr Gly Lys Ser 
    145                 150                 155 

cgc atc att ttt gcc cca ctt cag aaa caa acg ttc atc aca gtg ttt     1462 
Arg Ile Ile Phe Ala Pro Leu Gln Lys Gln Thr Phe Ile Thr Val Phe 
160                 165                 170                 175 

ata aca gga ttc cag ccg gat gtt ctt caa att agc gag aag agt cgt     1510 
Ile Thr Gly Phe Gln Pro Asp Val Leu Gln Ile Ser Glu Lys Ser Arg 
                180                 185                 190 

ttg atg ttt gcc atc tgc aat tcc acg aaa att ctt tcg att aat atg     1558 
Leu Met Phe Ala Ile Cys Asn Ser Thr Lys Ile Leu Ser Ile Asn Met 
            195                 200                 205 

cag gga gcc aca acc ccg aag gat tac tgg caa gtt gga aat gcg gac     1606 
Gln Gly Ala Thr Thr Pro Lys Asp Tyr Trp Gln Val Gly Asn Ala Asp 
        210                 215                 220 

tgc atg ggc tat cag agt tct ctc atg ctc acg acc gag gag gat aaa     1654 
Cys Met Gly Tyr Gln Ser Ser Leu Met Leu Thr Thr Glu Glu Asp Lys 
    225                 230                 235 

ctc ctc tac tac ggc ata tta aat gga acc cca tcc atc atg tct tta     1702 
Leu Leu Tyr Tyr Gly Ile Leu Asn Gly Thr Pro Ser Ile Met Ser Leu 
240                 245                 250                 255 

ccc gcc acc aaa acg aag acg gaa gca ccc aga att tgc ccg gat gtg     1750 
Pro Ala Thr Lys Thr Lys Thr Glu Ala Pro Arg Ile Cys Pro Asp Val 
                260                 265                 270 

ttg ttg cgg tgg cca cat ggg ccc att gtt tcg ctt gtg aat att aac     1798 
Leu Leu Arg Trp Pro His Gly Pro Ile Val Ser Leu Val Asn Ile Asn 
            275                 280                 285 

aaa cat gca ttt tac gtt gtt acc gcc tcc aat gta tac att gta cat     1846 
Lys His Ala Phe Tyr Val Val Thr Ala Ser Asn Val Tyr Ile Val His 
        290                 295                 300 

gat ggc tct tat cat ccg act gtg acg ccg aca cct cct ctg aca ccg     1894 
Asp Gly Ser Tyr His Pro Thr Val Thr Pro Thr Pro Pro Leu Thr Pro 
    305                 310                 315 

acg cct aca cca gaa gtg aca ccc aca cct act gtg acc ccg acg cct     1942 
Thr Pro Thr Pro Glu Val Thr Pro Thr Pro Thr Val Thr Pro Thr Pro 
320                 325                 330                 335 

aca ccg gaa gtg aca ccg aca ccg cca gtg act ccg agc ccc acc atc     1990 
Thr Pro Glu Val Thr Pro Thr Pro Pro Val Thr Pro Ser Pro Thr Ile 
                340                 345                 350 

aca atc cac cgg ggt ttt gct gtg gca gcc ttt cct gcc caa agt ctt     2038 
Thr Ile His Arg Gly Phe Ala Val Ala Ala Phe Pro Ala Gln Ser Leu 
            355                 360                 365 

cca atc gaa gac ccg cgg ctt atg cat gaa ctg ctt tct tgg tta atg     2086 
Pro Ile Glu Asp Pro Arg Leu Met His Glu Leu Leu Ser Trp Leu Met 
        370                 375                 380 

aag gat gta ggg att gcg ttc gaa tcc acg gac ttt ttt gcc gta ttt     2134 
Lys Asp Val Gly Ile Ala Phe Glu Ser Thr Asp Phe Phe Ala Val Phe 
    385                 390                 395 

cct cca gat aga gag gtt ttg gtg ccc ggt tat gta aat gtc tcc acc     2182 
Pro Pro Asp Arg Glu Val Leu Val Pro Gly Tyr Val Asn Val Ser Thr 
400                 405                 410                 415 

tgg aat aac ttg acg gtg cta ttc aac ttt gac cgc acc att gtc atc     2230 
Trp Asn Asn Leu Thr Val Leu Phe Asn Phe Asp Arg Thr Ile Val Ile 
                420                 425                 430 

acg gaa tat ttc act cca gag ggc atg tct tca gag gag gga cag gcc     2278 
Thr Glu Tyr Phe Thr Pro Glu Gly Met Ser Ser Glu Glu Gly Gln Ala 
            435                 440                 445 

cga ctc ttc gct tcg ccg tgg tac tgg acg aga aat ttc ctt gat tca     2326 
Arg Leu Phe Ala Ser Pro Trp Tyr Trp Thr Arg Asn Phe Leu Asp Ser 
        450                 455                 460 

tta aag aaa aca gta gct tgg aag gac ttg gag gcg ttt tgc atg gtc     2374 
Leu Lys Lys Thr Val Ala Trp Lys Asp Leu Glu Ala Phe Cys Met Val 
    465                 470                 475 

aac tgt gtt gaa cac tgt gag aca atg aca ttc cat aag tca gaa tgt     2422 
Asn Cys Val Glu His Cys Glu Thr Met Thr Phe His Lys Ser Glu Cys 
480                 485                 490                 495 

gta ggc tac gtc cgg ccc cca gta tgc aac gac gtc tgt gtg ggg gcg     2470 
Val Gly Tyr Val Arg Pro Pro Val Cys Asn Asp Val Cys Val Gly Ala 
                500                 505                 510 

gta gtg tcc tcc gtg gtg ctt ggc gcc aca ggt atc gca ctc att gca     2518 
Val Val Ser Ser Val Val Leu Gly Ala Thr Gly Ile Ala Leu Ile Ala 
            515                 520                 525 

ctg atg gtt gga agt tcg gcg aac tta cgg agc gct gtg att ctt gtt     2566 
Leu Met Val Gly Ser Ser Ala Asn Leu Arg Ser Ala Val Ile Leu Val 
        530                 535                 540 

cca ccc atg tagattttgt ccccacactt tggagaaagg tgggaaatga             2615 
Pro Pro Met 
    545 

cttcagaaat tgaaattaga aggaaccaac aacacaagaa gcaagcgaag gtgaaaacaa   2675 

cgggaagaag aagaagaaga aaaaaaaaaa aagaaaagaa aaaaatgggg ggctgagtgg   2735 

ggaaaagaga aagaaaagaa gtgtgcgtgt aaccgtgtgt gtgtgtgccg gggaaaaaga   2795 

agaaacacaa aagatttctt ttttgttttt tgttttaatg gtgcaaagag ggaaacaaga   2855 

aagcgaaggg tgcatgtgtg tctgtagata tataaaaata aacatatgcc cccgcatgta   2915 

ttttaccgtt ggcagttccg tggcttcttt tttttttttt tttttgtatt tttgttattt   2975 

tttcctctta tttcttcgtg tgtgtgtgta tgtattatta ttcttttttg ttttttgttt   3035 

gtttgtttgt ttttacctac tcatctgcct tcattttttt ttttgtgtgt tttcactcag   3095 

cccctctctc tttctctctt cttcttctct cttcatgcgt gtatttccgc atggagtgga   3155 

aaaggaacgg ctgggagcga ttgtgatggt gcttgtgttg gaggtgtggc tatgcgagta   3215 

gtggagatgc atgtatgtat gtatatatgt ggtttggtgt atatatttaa atattatatg   3275 

ttgttgttgt tgctgtccga ctctcggggg acgtacaccg acctacttac ttacagagag   3335 

agagagagag aggaagagaa tgagagaaaa ggggggcgtg tggtgtgttc tgtattcatt   3395 

gaagagcgca aaaataaata aaaataaaat aataaaatga gggagagaga agggaggagg   3455 

aaacagcaga ggaatttgta tgccatcgtt gtgactaatt tttcataagg actctgtgat   3515 

ggccctgtta accacgtcca ctgcagtaga cgagtcaaaa ttgactgcga gtgttacgcc   3575 

aactgtacgt ctgtctccct cgtgctgtac gtgtgcaagt aagtacgtgt gtgcactgtg   3635 

cgtgtgcgtg tgtgtgtgtg tcaagggcgc cttttacgtg tctgtgcgct tgagtgggga   3695 

ggggagaaga ggaggagaga cgaagaaaga aagaaagaaa aaagcgggcg gcgc         3749 

 
           
             34  
             581  
             PRT  
             T CRUZI  
           
            34 

Met Phe Ser Lys Arg Thr Ser Pro Ala Pro Phe Arg Ala Leu Leu Leu 
-35                 -30                 -25                 -20 

Pro Val Val Val Val Val Val Val Val Val Ala Ser Val Ala Leu Pro 
                -15                 -10                 -5 

Ala Gly Ala Gln Phe Asp Leu Arg Gln Gln Gln Leu Val Ile Gln Asp 
        -1  1               5                   10 

Phe Phe Ile Ser Arg Ser Cys Ala Gly Cys Ser Gln Gly Gln Thr Asp 
    15                  20                  25 

Gly Pro Ser Gly Ala Gly Thr Leu Phe Thr Ala Ala Gly Gly Ser Leu 
30                  35                  40                  45 

Gly Lys Asp Ala Ser Thr Leu Leu Leu Cys Asp Gln Gly Gly Gly Gly 
                50                  55                  60 

Ser Ser Val Arg Leu Val Asn Lys Ser Gly Ile Phe Thr Leu Ala Gly 
            65                  70                  75 

Ser Lys Thr Thr Arg Gly Asn Gln Asn Gly Pro Ala Ala Thr Ala Leu 
        80                  85                  90 

Phe Asn Met Pro Arg Ala Val Val Leu Glu Asp Gly Ala Leu Tyr Val 
    95                  100                 105 

Ala Asp Ser Ala Asn Asn Leu Val Arg Glu Ile Ser Asn Gly Ile Val 
110                 115                 120                 125 

Thr Ser Phe Ile Thr Glu Gly Leu Leu Gly Pro Ser Tyr Ile Lys Pro 
                130                 135                 140 

Tyr Ser Arg Pro Asn Gly Ala His Asp Leu Phe Val Ser Asp Thr Gly 
            145                 150                 155 

Lys Ser Arg Ile Ile Phe Ala Pro Leu Gln Lys Gln Thr Phe Ile Thr 
        160                 165                 170 

Val Phe Ile Thr Gly Phe Gln Pro Asp Val Leu Gln Ile Ser Glu Lys 
    175                 180                 185 

Ser Arg Leu Met Phe Ala Ile Cys Asn Ser Thr Lys Ile Leu Ser Ile 
190                 195                 200                 205 

Asn Met Gln Gly Ala Thr Thr Pro Lys Asp Tyr Trp Gln Val Gly Asn 
                210                 215                 220 

Ala Asp Cys Met Gly Tyr Gln Ser Ser Leu Met Leu Thr Thr Glu Glu 
            225                 230                 235 

Asp Lys Leu Leu Tyr Tyr Gly Ile Leu Asn Gly Thr Pro Ser Ile Met 
        240                 245                 250 

Ser Leu Pro Ala Thr Lys Thr Lys Thr Glu Ala Pro Arg Ile Cys Pro 
    255                 260                 265 

Asp Val Leu Leu Arg Trp Pro His Gly Pro Ile Val Ser Leu Val Asn 
270                 275                 280                 285 

Ile Asn Lys His Ala Phe Tyr Val Val Thr Ala Ser Asn Val Tyr Ile 
                290                 295                 300 

Val His Asp Gly Ser Tyr His Pro Thr Val Thr Pro Thr Pro Pro Leu 
            305                 310                 315 

Thr Pro Thr Pro Thr Pro Glu Val Thr Pro Thr Pro Thr Val Thr Pro 
        320                 325                 330 

Thr Pro Thr Pro Glu Val Thr Pro Thr Pro Pro Val Thr Pro Ser Pro 
    335                 340                 345 

Thr Ile Thr Ile His Arg Gly Phe Ala Val Ala Ala Phe Pro Ala Gln 
350                 355                 360                 365 

Ser Leu Pro Ile Glu Asp Pro Arg Leu Met His Glu Leu Leu Ser Trp 
                370                 375                 380 

Leu Met Lys Asp Val Gly Ile Ala Phe Glu Ser Thr Asp Phe Phe Ala 
            385                 390                 395 

Val Phe Pro Pro Asp Arg Glu Val Leu Val Pro Gly Tyr Val Asn Val 
        400                 405                 410 

Ser Thr Trp Asn Asn Leu Thr Val Leu Phe Asn Phe Asp Arg Thr Ile 
    415                 420                 425 

Val Ile Thr Glu Tyr Phe Thr Pro Glu Gly Met Ser Ser Glu Glu Gly 
430                 435                 440                 445 

Gln Ala Arg Leu Phe Ala Ser Pro Trp Tyr Trp Thr Arg Asn Phe Leu 
                450                 455                 460 

Asp Ser Leu Lys Lys Thr Val Ala Trp Lys Asp Leu Glu Ala Phe Cys 
            465                 470                 475 

Met Val Asn Cys Val Glu His Cys Glu Thr Met Thr Phe His Lys Ser 
        480                 485                 490 

Glu Cys Val Gly Tyr Val Arg Pro Pro Val Cys Asn Asp Val Cys Val 
    495                 500                 505 

Gly Ala Val Val Ser Ser Val Val Leu Gly Ala Thr Gly Ile Ala Leu 
510                 515                 520                 525 

Ile Ala Leu Met Val Gly Ser Ser Ala Asn Leu Arg Ser Ala Val Ile 
                530                 535                 540 

Leu Val Pro Pro Met 
            545 

 
           
             35  
             2151  
             DNA  
             T CRUZI  
             
               CDS  
               (1)..(2151)  
             
           
            35 

atg gcc cga gct gtg gtg ctt gag gat gga gcg ctt tac gtg gcg gac       48 
Met Ala Arg Ala Val Val Leu Glu Asp Gly Ala Leu Tyr Val Ala Asp 
1               5                   10                  15 

aat gcc aac aac ctc gtt cga gaa atc tcc aat ggc gtt gtc act tcg       96 
Asn Ala Asn Asn Leu Val Arg Glu Ile Ser Asn Gly Val Val Thr Ser 
            20                  25                  30 

ttt att acg gaa gga ctg ctg ggc cca tcg tac atc aaa ccg tac agc      144 
Phe Ile Thr Glu Gly Leu Leu Gly Pro Ser Tyr Ile Lys Pro Tyr Ser 
        35                  40                  45 

cgt aca aat ggc gct cat gac ttg ttt gtg tcg gac acg ggc aaa tca      192 
Arg Thr Asn Gly Ala His Asp Leu Phe Val Ser Asp Thr Gly Lys Ser 
    50                  55                  60 

cgc atc att ttt gcc cca cct cag aaa aaa acg ttc atc aca gtg ttt      240 
Arg Ile Ile Phe Ala Pro Pro Gln Lys Lys Thr Phe Ile Thr Val Phe 
65                  70                  75                  80 

ata aca gga ttc cag ccg gat gtt ctt caa att agc gag aag agt cgt      288 
Ile Thr Gly Phe Gln Pro Asp Val Leu Gln Ile Ser Glu Lys Ser Arg 
                85                  90                  95 

ttg atg ttt gcc atc tgc aat tcc acg aaa att ctt gcg att aat atg      336 
Leu Met Phe Ala Ile Cys Asn Ser Thr Lys Ile Leu Ala Ile Asn Met 
            100                 105                 110 

cag gga gcc aca acc ccg aag gag tac tgg caa gtt gga aat gcg gac      384 
Gln Gly Ala Thr Thr Pro Lys Glu Tyr Trp Gln Val Gly Asn Ala Asp 
        115                 120                 125 

tgc atg ggc tat cag agt tcc ctc atg ctc acg acc gag gag gat aaa      432 
Cys Met Gly Tyr Gln Ser Ser Leu Met Leu Thr Thr Glu Glu Asp Lys 
    130                 135                 140 

ctc ctc tac tac ggc ata tta aat gga acc cca tcc atc atg tct tta      480 
Leu Leu Tyr Tyr Gly Ile Leu Asn Gly Thr Pro Ser Ile Met Ser Leu 
145                 150                 155                 160 

ccc gcc acc aaa acg aag acg gaa gca ccc aga att tgc ccg gat gtg      528 
Pro Ala Thr Lys Thr Lys Thr Glu Ala Pro Arg Ile Cys Pro Asp Val 
                165                 170                 175 

ttg ttg cag tgg cca cat ggg ccc att gtt tcg ctt gtg aat att aac      576 
Leu Leu Gln Trp Pro His Gly Pro Ile Val Ser Leu Val Asn Ile Asn 
            180                 185                 190 

aaa cat gca ttt tac gtt gtt acc gcc tcc aat gta tac att gta cat      624 
Lys His Ala Phe Tyr Val Val Thr Ala Ser Asn Val Tyr Ile Val His 
        195                 200                 205 

gat ggc tcg tat cat ccg act gga tcc atg gcc cag ctc caa cag gca      672 
Asp Gly Ser Tyr His Pro Thr Gly Ser Met Ala Gln Leu Gln Gln Ala 
    210                 215                 220 

gaa aat aat atc act aat tcc aaa aaa gaa atg aca aag cta cga gaa      720 
Glu Asn Asn Ile Thr Asn Ser Lys Lys Glu Met Thr Lys Leu Arg Glu 
225                 230                 235                 240 

aaa gtg aaa aag gcc gag aaa gaa aaa ttg gac gcc att aac cgg gca      768 
Lys Val Lys Lys Ala Glu Lys Glu Lys Leu Asp Ala Ile Asn Arg Ala 
                245                 250                 255 

acc aag ctg gaa gag gaa cga aac caa gcg tac aaa gca gca cac aag      816 
Thr Lys Leu Glu Glu Glu Arg Asn Gln Ala Tyr Lys Ala Ala His Lys 
            260                 265                 270 

gca gag gag gaa aag gct aaa aca ttt caa cgc ctt ata aca ttt gag      864 
Ala Glu Glu Glu Lys Ala Lys Thr Phe Gln Arg Leu Ile Thr Phe Glu 
        275                 280                 285 

tcg gaa aat att aac tta aag aaa agg cca aat gac gca gtt tca aat      912 
Ser Glu Asn Ile Asn Leu Lys Lys Arg Pro Asn Asp Ala Val Ser Asn 
    290                 295                 300 

cgg gat aag aaa aaa aat tct gaa acc gca aaa act gac gaa gta gag      960 
Arg Asp Lys Lys Lys Asn Ser Glu Thr Ala Lys Thr Asp Glu Val Glu 
305                 310                 315                 320 

aaa cag agg gcg gct gag gct gcc aag gcc gtg gag acg gag aag cag     1008 
Lys Gln Arg Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln 
                325                 330                 335 

agg gca gct gag gcc acg aag gtt gcc gaa gcg gag aag cgg aag gca     1056 
Arg Ala Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Arg Lys Ala 
            340                 345                 350 

gct gag gcc gcc aag gcc gtg gag acg gag aag cag agg gca gct gaa     1104 
Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu 
        355                 360                 365 

gcc acg aag gtt gcc gaa gcg gag aag cag aag gca gct gag gcc gcc     1152 
Ala Thr Lys Val Ala Glu Ala Glu Lys Gln Lys Ala Ala Glu Ala Ala 
    370                 375                 380 

aag gcc gtg gag acg gag aag cag agg gca gct gaa gcc acg aag gtt     1200 
Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu Ala Thr Lys Val 
385                 390                 395                 400 

gcc gaa gcg gag aag cag agg gca gct gaa gcc atg aag gtt gcc gaa     1248 
Ala Glu Ala Glu Lys Gln Arg Ala Ala Glu Ala Met Lys Val Ala Glu 
                405                 410                 415 

gcg gag aag cag aag gca gct gag gcc gcc aag gcc gtg gag acg gag     1296 
Ala Glu Lys Gln Lys Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu 
            420                 425                 430 

aag cag agg gca gct gaa gcc acg aag gtt gcc gaa gcg gag aag cag     1344 
Lys Gln Arg Ala Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Gln 
        435                 440                 445 

aag gca gct gag gcc gcc aag gcc gtg gag acg gag aag cag agg gca     1392 
Lys Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala 
    450                 455                 460 

gct gaa gcc acg aag gtt gcc gaa gcg gag aag cag aag gca gct gag     1440 
Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Gln Lys Ala Ala Glu 
465                 470                 475                 480 

gcc gcc aag gcc gtg gag acg gag aag cag agg gca gct gaa gcc acg     1488 
Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu Ala Thr 
                485                 490                 495 

aag gtt gcc gaa gcg gag aag gat atc gat ccc atg ggt gct tgt ggg     1536 
Lys Val Ala Glu Ala Glu Lys Asp Ile Asp Pro Met Gly Ala Cys Gly 
            500                 505                 510 

tcg aag gac tcg acg agc gac aag ggg ttg gcg agc gat aag gac ggc     1584 
Ser Lys Asp Ser Thr Ser Asp Lys Gly Leu Ala Ser Asp Lys Asp Gly 
        515                 520                 525 

aag aac gcc aag gac cgc aag gaa gcg tgg gag cgc att cgc cag gcg     1632 
Lys Asn Ala Lys Asp Arg Lys Glu Ala Trp Glu Arg Ile Arg Gln Ala 
    530                 535                 540 

att cct cgt gag aag acc gcc gag gca aaa cag cgc cgc atc gag ctc     1680 
Ile Pro Arg Glu Lys Thr Ala Glu Ala Lys Gln Arg Arg Ile Glu Leu 
545                 550                 555                 560 

ttc aag aag ttc gac aag aac gag acc ggg aag ctg tgc tac gat gag     1728 
Phe Lys Lys Phe Asp Lys Asn Glu Thr Gly Lys Leu Cys Tyr Asp Glu 
                565                 570                 575 

gtg cac agc ggc tgc ctc gag gtg ctg aag ttg gac gag ttc acg ccg     1776 
Val His Ser Gly Cys Leu Glu Val Leu Lys Leu Asp Glu Phe Thr Pro 
            580                 585                 590 

cga gtg cgc gac atc acg aag cgt gca ttc gac aag gcg agg gcc ctg     1824 
Arg Val Arg Asp Ile Thr Lys Arg Ala Phe Asp Lys Ala Arg Ala Leu 
        595                 600                 605 

ggc agc aag ctg gag aac aag ggc tcc gag gac ttt gtt gaa ttt ctg     1872 
Gly Ser Lys Leu Glu Asn Lys Gly Ser Glu Asp Phe Val Glu Phe Leu 
    610                 615                 620 

gag ttc cgt ctg atg ctg tgc tac atc tac gac ttc ttc gag ctg acg     1920 
Glu Phe Arg Leu Met Leu Cys Tyr Ile Tyr Asp Phe Phe Glu Leu Thr 
625                 630                 635                 640 

gtg atg ttc gac gag att gac gcc tcc ggc aac atg ctg gtt gac gag     1968 
Val Met Phe Asp Glu Ile Asp Ala Ser Gly Asn Met Leu Val Asp Glu 
                645                 650                 655 

gag gag ttc aag cgc gcc gtg ccc aag ctt gag gcg tgg ggc gcc aag     2016 
Glu Glu Phe Lys Arg Ala Val Pro Lys Leu Glu Ala Trp Gly Ala Lys 
            660                 665                 670 

gtc gag gat ccc gcg gcg ctg ttc aag gag ctc gat aag aac ggc act     2064 
Val Glu Asp Pro Ala Ala Leu Phe Lys Glu Leu Asp Lys Asn Gly Thr 
        675                 680                 685 

ggg tcc gtg acg ttc gac gag ttt gct gcg tgg gct tct gca gtc aaa     2112 
Gly Ser Val Thr Phe Asp Glu Phe Ala Ala Trp Ala Ser Ala Val Lys 
    690                 695                 700 

ctg gac gcc gac ggc gac ccg gac aac gtg ccg gat atc                 2151 
Leu Asp Ala Asp Gly Asp Pro Asp Asn Val Pro Asp Ile 
705                 710                 715 

 
           
             36  
             717  
             PRT  
             T CRUZI  
           
            36 

Met Ala Arg Ala Val Val Leu Glu Asp Gly Ala Leu Tyr Val Ala Asp 
1               5                   10                  15 

Asn Ala Asn Asn Leu Val Arg Glu Ile Ser Asn Gly Val Val Thr Ser 
            20                  25                  30 

Phe Ile Thr Glu Gly Leu Leu Gly Pro Ser Tyr Ile Lys Pro Tyr Ser 
        35                  40                  45 

Arg Thr Asn Gly Ala His Asp Leu Phe Val Ser Asp Thr Gly Lys Ser 
    50                  55                  60 

Arg Ile Ile Phe Ala Pro Pro Gln Lys Lys Thr Phe Ile Thr Val Phe 
65                  70                  75                  80 

Ile Thr Gly Phe Gln Pro Asp Val Leu Gln Ile Ser Glu Lys Ser Arg 
                85                  90                  95 

Leu Met Phe Ala Ile Cys Asn Ser Thr Lys Ile Leu Ala Ile Asn Met 
            100                 105                 110 

Gln Gly Ala Thr Thr Pro Lys Glu Tyr Trp Gln Val Gly Asn Ala Asp 
        115                 120                 125 

Cys Met Gly Tyr Gln Ser Ser Leu Met Leu Thr Thr Glu Glu Asp Lys 
    130                 135                 140 

Leu Leu Tyr Tyr Gly Ile Leu Asn Gly Thr Pro Ser Ile Met Ser Leu 
145                 150                 155                 160 

Pro Ala Thr Lys Thr Lys Thr Glu Ala Pro Arg Ile Cys Pro Asp Val 
                165                 170                 175 

Leu Leu Gln Trp Pro His Gly Pro Ile Val Ser Leu Val Asn Ile Asn 
            180                 185                 190 

Lys His Ala Phe Tyr Val Val Thr Ala Ser Asn Val Tyr Ile Val His 
        195                 200                 205 

Asp Gly Ser Tyr His Pro Thr Gly Ser Met Ala Gln Leu Gln Gln Ala 
    210                 215                 220 

Glu Asn Asn Ile Thr Asn Ser Lys Lys Glu Met Thr Lys Leu Arg Glu 
225                 230                 235                 240 

Lys Val Lys Lys Ala Glu Lys Glu Lys Leu Asp Ala Ile Asn Arg Ala 
                245                 250                 255 

Thr Lys Leu Glu Glu Glu Arg Asn Gln Ala Tyr Lys Ala Ala His Lys 
            260                 265                 270 

Ala Glu Glu Glu Lys Ala Lys Thr Phe Gln Arg Leu Ile Thr Phe Glu 
        275                 280                 285 

Ser Glu Asn Ile Asn Leu Lys Lys Arg Pro Asn Asp Ala Val Ser Asn 
    290                 295                 300 

Arg Asp Lys Lys Lys Asn Ser Glu Thr Ala Lys Thr Asp Glu Val Glu 
305                 310                 315                 320 

Lys Gln Arg Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln 
                325                 330                 335 

Arg Ala Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Arg Lys Ala 
            340                 345                 350 

Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu 
        355                 360                 365 

Ala Thr Lys Val Ala Glu Ala Glu Lys Gln Lys Ala Ala Glu Ala Ala 
    370                 375                 380 

Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu Ala Thr Lys Val 
385                 390                 395                 400 

Ala Glu Ala Glu Lys Gln Arg Ala Ala Glu Ala Met Lys Val Ala Glu 
                405                 410                 415 

Ala Glu Lys Gln Lys Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu 
            420                 425                 430 

Lys Gln Arg Ala Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Gln 
        435                 440                 445 

Lys Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala 
    450                 455                 460 

Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Gln Lys Ala Ala Glu 
465                 470                 475                 480 

Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu Ala Thr 
                485                 490                 495 

Lys Val Ala Glu Ala Glu Lys Asp Ile Asp Pro Met Gly Ala Cys Gly 
            500                 505                 510 

Ser Lys Asp Ser Thr Ser Asp Lys Gly Leu Ala Ser Asp Lys Asp Gly 
        515                 520                 525 

Lys Asn Ala Lys Asp Arg Lys Glu Ala Trp Glu Arg Ile Arg Gln Ala 
    530                 535                 540 

Ile Pro Arg Glu Lys Thr Ala Glu Ala Lys Gln Arg Arg Ile Glu Leu 
545                 550                 555                 560 

Phe Lys Lys Phe Asp Lys Asn Glu Thr Gly Lys Leu Cys Tyr Asp Glu 
                565                 570                 575 

Val His Ser Gly Cys Leu Glu Val Leu Lys Leu Asp Glu Phe Thr Pro 
            580                 585                 590 

Arg Val Arg Asp Ile Thr Lys Arg Ala Phe Asp Lys Ala Arg Ala Leu 
        595                 600                 605 

Gly Ser Lys Leu Glu Asn Lys Gly Ser Glu Asp Phe Val Glu Phe Leu 
    610                 615                 620 

Glu Phe Arg Leu Met Leu Cys Tyr Ile Tyr Asp Phe Phe Glu Leu Thr 
625                 630                 635                 640 

Val Met Phe Asp Glu Ile Asp Ala Ser Gly Asn Met Leu Val Asp Glu 
                645                 650                 655 

Glu Glu Phe Lys Arg Ala Val Pro Lys Leu Glu Ala Trp Gly Ala Lys 
            660                 665                 670 

Val Glu Asp Pro Ala Ala Leu Phe Lys Glu Leu Asp Lys Asn Gly Thr 
        675                 680                 685 

Gly Ser Val Thr Phe Asp Glu Phe Ala Ala Trp Ala Ser Ala Val Lys 
    690                 695                 700 

Leu Asp Ala Asp Gly Asp Pro Asp Asn Val Pro Asp Ile 
705                 710                 715 

 
           
             37  
             1836  
             DNA  
             T CRUZI  
             
               CDS  
               (1)..(1836)  
             
           
            37 

atg gag cag gag cgc agg cag ctg ctc gag aag gac ccg cgc agg aac       48 
Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15 

gcg aag gag atc gct gcg ctt gag gag agc atg aat gcc cgc gca cag       96 
Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30 

gag ctg gca cgc gag aag aag ctt gct gac cgc gcg ttc ctc gac cag      144 
Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45 

aag ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc gac gac gac agc      192 
Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60 

gac ttt gtt gct atg gag cag gag cgc agg cag ctg ctc gag aag gac      240 
Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp 
65                  70                  75                  80 

ccg cgc agg aac gcg aag gag atc gct gcg ctt gag gag agc atg aat      288 
Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn 
                85                  90                  95 

gcc cgc gca cag gag ctg gca cgc gag aag aag ctt gct gac cgc gcg      336 
Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala 
            100                 105                 110 

ttc ctc gac cag aag ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc      384 
Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu 
        115                 120                 125 

gac gac gac agc gac ttt gtt gct atg gag cag gag cgc agg cag ctg      432 
Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu 
    130                 135                 140 

ctc gag aag gac ccg cgc agg aac gcg aag gag atc gct gcg ctt gag      480 
Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu 
145                 150                 155                 160 

gag agc atg aat gcc cgc gca cag gag ctg gca cgc gag aag aag ctt      528 
Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu 
                165                 170                 175 

gct gac cgc gcg ttc ctc gac cag aag ccg gag ggc gtg ccg ctg cga      576 
Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg 
            180                 185                 190 

gag ctg ccg ctc gac gac gac agc gac ttt gtt gct atg gag cag gag      624 
Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu 
        195                 200                 205 

cgc agg cag ctg ctc gag aag gac ccg cgc agg aac gcg aag gag atc      672 
Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile 
    210                 215                 220 

gct gcg ctt gag gag agc atg aat gcc cgc gca cag gag ctg gca cgc      720 
Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg 
225                 230                 235                 240 

gag aag aag ctt gct gac cgc gcg ttc ctc gac cag aag ccg gag ggc      768 
Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly 
                245                 250                 255 

gtg ccg ctg cga gag ctg ccg ctc gac gac gac agc gac ttt gtt gct      816 
Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala 
            260                 265                 270 

atg gag cag gag cgc agg cag ctg ctc gag aag gac ccg cgc agg aac      864 
Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
        275                 280                 285 

gcg aag gag atc gct gcg ctt gag gag agc atg aat gcc cgc gca cag      912 
Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
    290                 295                 300 

gag ctg gca cgc gag aag aag ctt gct gac cgc gcg ttc ctc gac cag      960 
Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
305                 310                 315                 320 

aag ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc gac gac gac agc     1008 
Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
                325                 330                 335 

gac ttt gtt gct atg gag cag gag cgc agg cag ctg ctc gag aag gac     1056 
Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp 
            340                 345                 350 

ccg cgc agg aac gcg aag gag atc gct gcg ctt gag gag agc atg aat     1104 
Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn 
        355                 360                 365 

gcc cgc gca cag gag ctg gca cgc gag aag aag ctt gct gac cgc gcg     1152 
Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala 
    370                 375                 380 

ttc ctc gac cag aag ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc     1200 
Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu 
385                 390                 395                 400 

gac gac gac agc gac ttt gtt gct atg gag cag gag cgc agg cag ctg     1248 
Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu 
                405                 410                 415 

ctc gag aag gac ccg cgc agg aac gcg aag gag atc gct gcg ctt gag     1296 
Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu 
            420                 425                 430 

gag agc atg aat gcc cgc gca cag gag ctg gca cgc gag aag aag ctt     1344 
Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu 
        435                 440                 445 

gct gac cgc gcg ttc ctc gac cag aag ccg gag ggc gtg ccg ctg cga     1392 
Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg 
    450                 455                 460 

gag ctg ccg ctc gac gac gac agc gac ttt gtt gct atg gag cag gag     1440 
Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu 
465                 470                 475                 480 

cgc agg cag ctg ctc gag aag gac ccg cgc agg aac gcg aag gag atc     1488 
Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile 
                485                 490                 495 

gct gcg ctt gag gag agc atg aat gcc cgc gca cag gag ctg gca cgc     1536 
Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg 
            500                 505                 510 

gag aag aag ctt gct gac cgc gcg ttc ctc gac cag aag ccg gag ggc     1584 
Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly 
        515                 520                 525 

gtg ccg ctg cga gag ctg ccg ctc gac gac gac agc gac ttt gtt gct     1632 
Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala 
    530                 535                 540 

atg gag cag gag cgc agg cag ctg ctc gag aag gac ccg cgc agg aac     1680 
Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
545                 550                 555                 560 

gcg aag gag atc gct gcg ctt gag gag agc atg aat gcc cgc gca cag     1728 
Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
                565                 570                 575 

gag ctg gca cgc gag aag aag ctt gct gac cgc gcg ttc ctc gac cag     1776 
Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
            580                 585                 590 

aag ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc gac gac gac agc     1824 
Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
        595                 600                 605 

gac ttt gtt gct                                                     1836 
Asp Phe Val Ala 
    610 

 
           
             38  
             612  
             PRT  
             T CRUZI  
           
            38 

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
1               5                   10                  15 

Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
            20                  25                  30 

Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
        35                  40                  45 

Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
    50                  55                  60 

Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp 
65                  70                  75                  80 

Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn 
                85                  90                  95 

Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala 
            100                 105                 110 

Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu 
        115                 120                 125 

Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu 
    130                 135                 140 

Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu 
145                 150                 155                 160 

Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu 
                165                 170                 175 

Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg 
            180                 185                 190 

Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu 
        195                 200                 205 

Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile 
    210                 215                 220 

Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg 
225                 230                 235                 240 

Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly 
                245                 250                 255 

Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala 
            260                 265                 270 

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
        275                 280                 285 

Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
    290                 295                 300 

Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
305                 310                 315                 320 

Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
                325                 330                 335 

Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp 
            340                 345                 350 

Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn 
        355                 360                 365 

Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala 
    370                 375                 380 

Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu 
385                 390                 395                 400 

Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu 
                405                 410                 415 

Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu 
            420                 425                 430 

Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu 
        435                 440                 445 

Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg 
    450                 455                 460 

Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu 
465                 470                 475                 480 

Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile 
                485                 490                 495 

Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg 
            500                 505                 510 

Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly 
        515                 520                 525 

Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala 
    530                 535                 540 

Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn 
545                 550                 555                 560 

Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln 
                565                 570                 575 

Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln 
            580                 585                 590 

Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser 
        595                 600                 605 

Asp Phe Val Ala 
    610 

 
           
             39  
             621  
             DNA  
             T CRUZI  
             
               CDS  
               (1)..(621)  
             
           
            39 

ttt aat cct tct acg gac aaa ttg aag cta aac caa caa aat aag cct       48 
Phe Asn Pro Ser Thr Asp Lys Leu Lys Leu Asn Gln Gln Asn Lys Pro 
1               5                   10                  15 

cat att gca aat aat aaa caa aaa aca aca ctc gaa aaa act caa aca       96 
His Ile Ala Asn Asn Lys Gln Lys Thr Thr Leu Glu Lys Thr Gln Thr 
            20                  25                  30 

gaa caa aaa aca gcg cca ttt gga cag ggc gca gca ggg tgg aca aaa      144 
Glu Gln Lys Thr Ala Pro Phe Gly Gln Gly Ala Ala Gly Trp Thr Lys 
        35                  40                  45 

cca tca cca ttt gga cag gcc gca gca ggt gac aaa cca cca cca ttt      192 
Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
    50                  55                  60 

gga cag gcc gca gca ggt gac aaa cca cca cca ttt gga cag gcc gca      240 
Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe Gly Gln Ala Ala 
65                  70                  75                  80 

gca ggt gac aaa cca tca cta ttt gga cag gcc gca gca ggt gac aaa      288 
Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala Ala Gly Asp Lys 
                85                  90                  95 

cca tca cca ttt gga cag gcc gca gca ggt gac aaa cca cca cca ttt      336 
Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
            100                 105                 110 

gga cag gcc gca gca ggt gac aaa cca tca cta ttt gga cag gcc gca      384 
Gly Gln Ala Ala Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala 
        115                 120                 125 

gca ggt gac aaa cca tca cca ttt gga cag gcc gca gca ggt gac aaa      432 
Ala Gly Asp Lys Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys 
    130                 135                 140 

cca cca cca ttt gga cag gcc gca gca ggt gac aaa cca cca cca ttt      480 
Pro Pro Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
145                 150                 155                 160 

gga cag gcc gca gca ggt gac aaa cca tca cta ttt gga cag gcc gca      528 
Gly Gln Ala Ala Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala 
                165                 170                 175 

gca ggt gac aaa cca tca cca ttt gga cag gga act gcg ttt gat gcc      576 
Ala Gly Asp Lys Pro Ser Pro Phe Gly Gln Gly Thr Ala Phe Asp Ala 
            180                 185                 190 

tct cga agc act gtg ttt gcg aat gcg cct ggt gtt gcc cag gtg          621 
Ser Arg Ser Thr Val Phe Ala Asn Ala Pro Gly Val Ala Gln Val 
        195                 200                 205 

 
           
             40  
             207  
             PRT  
             T CRUZI  
           
            40 

Phe Asn Pro Ser Thr Asp Lys Leu Lys Leu Asn Gln Gln Asn Lys Pro 
1               5                   10                  15 

His Ile Ala Asn Asn Lys Gln Lys Thr Thr Leu Glu Lys Thr Gln Thr 
            20                  25                  30 

Glu Gln Lys Thr Ala Pro Phe Gly Gln Gly Ala Ala Gly Trp Thr Lys 
        35                  40                  45 

Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
    50                  55                  60 

Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe Gly Gln Ala Ala 
65                  70                  75                  80 

Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala Ala Gly Asp Lys 
                85                  90                  95 

Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
            100                 105                 110 

Gly Gln Ala Ala Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala 
        115                 120                 125 

Ala Gly Asp Lys Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys 
    130                 135                 140 

Pro Pro Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
145                 150                 155                 160 

Gly Gln Ala Ala Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala 
                165                 170                 175 

Ala Gly Asp Lys Pro Ser Pro Phe Gly Gln Gly Thr Ala Phe Asp Ala 
            180                 185                 190 

Ser Arg Ser Thr Val Phe Ala Asn Ala Pro Gly Val Ala Gln Val 
        195                 200                 205 

 
           
             41  
             1845  
             DNA  
             T CRUZI  
             
               CDS  
               (1)..(1845)  
             
           
            41 

ttt aat cct tct acg gac aaa ttg aag cta aac caa caa aat aag cct       48 
Phe Asn Pro Ser Thr Asp Lys Leu Lys Leu Asn Gln Gln Asn Lys Pro 
1               5                   10                  15 

at att gca aat aat aaa caa aaa aca aca ctc gaa aaa act caa aca        96 
His Ile Ala Asn Asn Lys Gln Lys Thr Thr Leu Glu Lys Thr Gln Thr 
            20                  25                  30 

gaa caa aaa aca gcg cca ttt gga cag ggc gca gca ggg tgg aca aaa      144 
Glu Gln Lys Thr Ala Pro Phe Gly Gln Gly Ala Ala Gly Trp Thr Lys 
        35                  40                  45 

cca tca cca ttt gga cag gcc gca gca ggt gac aaa cca cca cca ttt      192 
Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
    50                  55                  60 

gga cag gcc gca gca ggt gac aaa cca cca cca ttt gga cag gcc gca      240 
Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe Gly Gln Ala Ala 
65                  70                  75                  80 

gca ggt gac aaa cca tca cta ttt gga cag gcc gca gca ggt gac aaa      288 
Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala Ala Gly Asp Lys 
                85                  90                  95 

cca tca cca ttt gga cag gcc gca gca ggt gac aaa cca cca cca ttt      336 
Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
            100                 105                 110 

gga cag gcc gca gca ggt gac aaa cca tca cta ttt gga cag gcc gca      384 
Gly Gln Ala Ala Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala 
        115                 120                 125 

gca ggt gac aaa cca tca cca ttt gga cag gcc gca gca ggt gac aaa      432 
Ala Gly Asp Lys Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys 
    130                 135                 140 

cca cca cca ttt gga cag gcc gca gca ggt gac aaa cca cca cca ttt      480 
Pro Pro Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
145                 150                 155                 160 

gga cag gcc gca gca ggt gac aaa cca tca cta ttt gga cag gcc gca      528 
Gly Gln Ala Ala Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala 
                165                 170                 175 

gca ggt gac aaa cca tca cca ttt gga cag gga act gcg ttt gat gcc      576 
Ala Gly Asp Lys Pro Ser Pro Phe Gly Gln Gly Thr Ala Phe Asp Ala 
            180                 185                 190 

tct cga agc act gtg ttt gcg aat gcg cct ggt gtt gcc cag gtg atg      624 
Ser Arg Ser Thr Val Phe Ala Asn Ala Pro Gly Val Ala Gln Val Met 
        195                 200                 205 

gag cag gag cgc agg cag ctg ctc gag aag gac ccg cgc agg aac gcg      672 
Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala 
    210                 215                 220 

aag gag atc gct gcg ctt gag gag agc atg aat gcc cgc gca cag gag      720 
Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu 
225                 230                 235                 240 

ctg gca cgc gag aag aag ctt gct gac cgc gcg ttc ctc gac cag aag      768 
Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys 
                245                 250                 255 

ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc gac gac gac agc gac      816 
Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp 
            260                 265                 270 

ttt gtt gct atg gag cag gag cgc agg cag ctg ctc gag aag gac ccg      864 
Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro 
        275                 280                 285 

cgc agg aac gcg aag gag atc gct gcg ctt gag gag agc atg aat gcc      912 
Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala 
    290                 295                 300 

cgc gca cag gag ctg gca cgc gag aag aag ctt gct gac cgc gcg ttc      960 
Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe 
305                 310                 315                 320 

ctc gac cag aag ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc gac     1008 
Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp 
                325                 330                 335 

gac gac agc gac ttt gtt gct atg gag cag gag cgc agg cag ctg ctc     1056 
Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu 
            340                 345                 350 

gag aag gac ccg cgc agg aac gcg aag gag atc gct gcg ctt gag gag     1104 
Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu 
        355                 360                 365 

agc atg aat gcc cgc gca cag gag ctg gca cgc gag aag aag ctt gct     1152 
Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala 
    370                 375                 380 

gac cgc gcg ttc ctc gac cag aag ccg gag ggc gtg ccg ctg cga gag     1200 
Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu 
385                 390                 395                 400 

ctg ccg ctc gac gac gac agc gac ttt gtt gct atg gag cag gag cgc     1248 
Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu Arg 
                405                 410                 415 

agg cag ctg ctc gag aag gac ccg cgc agg aac gcg aag gag atc gct     1296 
Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile Ala 
            420                 425                 430 

gcg ctt gag gag agc atg aat gcc cgc gca cag gag ctg gca cgc gag     1344 
Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu 
        435                 440                 445 

aag aag ctt gct gac cgc gcg ttc ctc gac cag aag ccg gag ggc gtg     1392 
Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val 
    450                 455                 460 

ccg ctg cga gag ctg ccg ctc gac gac gac agc gac ttt gtt gct atg     1440 
Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala Met 
465                 470                 475                 480 

gag cag gag cgc agg cag ctg ctc gag aag gac ccg cgc agg aac gcg     1488 
Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala 
                485                 490                 495 

aag gag atc gct gcg ctt gag gag agc atg aat gcc cgc gca cag gag     1536 
Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu 
            500                 505                 510 

ctg gca cgc gag aag aag ctt gct gac cgc gcg ttc ctc gac cag aag     1584 
Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys 
        515                 520                 525 

ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc gac gac gac agc gac     1632 
Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp 
    530                 535                 540 

ttt gtt gct atg gag cag gag cgc agg cag ctg ctc gag aag gac ccg     1680 
Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro 
545                 550                 555                 560 

cgc agg aac gcg aag gag atc gct gcg ctt gag gag agc atg aat gcc     1728 
Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala 
                565                 570                 575 

cgc gca cag gag ctg gca cgc gag aag aag ctt gct gac cgc gcg ttc     1776 
Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe 
            580                 585                 590 

ctc gac cag aag ccg gag ggc gtg ccg ctg cga gag ctg ccg ctc gac     1824 
Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp 
        595                 600                 605 

gac gac agc gac ttt gtt gct                                         1845 
Asp Asp Ser Asp Phe Val Ala 
    610                 615 

 
           
             42  
             615  
             PRT  
             T CRUZI  
           
            42 

Phe Asn Pro Ser Thr Asp Lys Leu Lys Leu Asn Gln Gln Asn Lys Pro 
1               5                   10                  15 

His Ile Ala Asn Asn Lys Gln Lys Thr Thr Leu Glu Lys Thr Gln Thr 
            20                  25                  30 

Glu Gln Lys Thr Ala Pro Phe Gly Gln Gly Ala Ala Gly Trp Thr Lys 
        35                  40                  45 

Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
    50                  55                  60 

Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe Gly Gln Ala Ala 
65                  70                  75                  80 

Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala Ala Gly Asp Lys 
                85                  90                  95 

Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
            100                 105                 110 

Gly Gln Ala Ala Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala 
        115                 120                 125 

Ala Gly Asp Lys Pro Ser Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys 
    130                 135                 140 

Pro Pro Pro Phe Gly Gln Ala Ala Ala Gly Asp Lys Pro Pro Pro Phe 
145                 150                 155                 160 

Gly Gln Ala Ala Ala Gly Asp Lys Pro Ser Leu Phe Gly Gln Ala Ala 
                165                 170                 175 

Ala Gly Asp Lys Pro Ser Pro Phe Gly Gln Gly Thr Ala Phe Asp Ala 
            180                 185                 190 

Ser Arg Ser Thr Val Phe Ala Asn Ala Pro Gly Val Ala Gln Val Met 
        195                 200                 205 

Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala 
    210                 215                 220 

Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu 
225                 230                 235                 240 

Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys 
                245                 250                 255 

Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp 
            260                 265                 270 

Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro 
        275                 280                 285 

Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala 
    290                 295                 300 

Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe 
305                 310                 315                 320 

Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp 
                325                 330                 335 

Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu 
            340                 345                 350 

Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu 
        355                 360                 365 

Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala 
    370                 375                 380 

Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu 
385                 390                 395                 400 

Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala Met Glu Gln Glu Arg 
                405                 410                 415 

Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala Lys Glu Ile Ala 
            420                 425                 430 

Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu Leu Ala Arg Glu 
        435                 440                 445 

Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys Pro Glu Gly Val 
    450                 455                 460 

Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp Phe Val Ala Met 
465                 470                 475                 480 

Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro Arg Arg Asn Ala 
                485                 490                 495 

Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala Arg Ala Gln Glu 
            500                 505                 510 

Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe Leu Asp Gln Lys 
        515                 520                 525 

Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp Asp Asp Ser Asp 
    530                 535                 540 

Phe Val Ala Met Glu Gln Glu Arg Arg Gln Leu Leu Glu Lys Asp Pro 
545                 550                 555                 560 

Arg Arg Asn Ala Lys Glu Ile Ala Ala Leu Glu Glu Ser Met Asn Ala 
                565                 570                 575 

Arg Ala Gln Glu Leu Ala Arg Glu Lys Lys Leu Ala Asp Arg Ala Phe 
            580                 585                 590 

Leu Asp Gln Lys Pro Glu Gly Val Pro Leu Arg Glu Leu Pro Leu Asp 
        595                 600                 605 

Asp Asp Ser Asp Phe Val Ala 
    610                 615 

 
           
             43  
             858  
             DNA  
             T CRUZI  
             
               CDS  
               (1)..(858)  
             
           
            43 

gat cca acg tat cgt ttt gca aac cac gcg ttc acg ctg gtg gcg tcg       48 
Asp Pro Thr Tyr Arg Phe Ala Asn His Ala Phe Thr Leu Val Ala Ser 
1               5                   10                  15 

gtg acg att cac gag gtt ccg agc gtc gcg agt cct ttg ctg ggt gcg       96 
Val Thr Ile His Glu Val Pro Ser Val Ala Ser Pro Leu Leu Gly Ala 
            20                  25                  30 

agc ctg gac tct tct ggt ggc aaa aaa ctc ctg ggg ctc tcg tac gac      144 
Ser Leu Asp Ser Ser Gly Gly Lys Lys Leu Leu Gly Leu Ser Tyr Asp 
        35                  40                  45 

gag aag cac cag tgg cag cca ata tac gga tca acg ccg gtg acg ccg      192 
Glu Lys His Gln Trp Gln Pro Ile Tyr Gly Ser Thr Pro Val Thr Pro 
    50                  55                  60 

acc gga tcg tgg gag atg ggt aag agg tac cac gtg gtt ctt acg atg      240 
Thr Gly Ser Trp Glu Met Gly Lys Arg Tyr His Val Val Leu Thr Met 
65                  70                  75                  80 

gcg aat aaa att ggc tcc gtg tac att gat gga gaa cct ctg gag ggt      288 
Ala Asn Lys Ile Gly Ser Val Tyr Ile Asp Gly Glu Pro Leu Glu Gly 
                85                  90                  95 

tca ggg cag acc gtt gtg cca gac gag agg acg cct gac atc tcc cac      336 
Ser Gly Gln Thr Val Val Pro Asp Glu Arg Thr Pro Asp Ile Ser His 
            100                 105                 110 

ttc tac gtt ggc ggg tat gga agg agt gat atg cca acc ata agc cac      384 
Phe Tyr Val Gly Gly Tyr Gly Arg Ser Asp Met Pro Thr Ile Ser His 
        115                 120                 125 

gtg acg gtg aat aat gtt ctt ctt tac aac cgt cag ctg aat gcc gag      432 
Val Thr Val Asn Asn Val Leu Leu Tyr Asn Arg Gln Leu Asn Ala Glu 
    130                 135                 140 

gag atc agg acc ttg ttc ttg agc cag gac ctg att ggc acg gaa gca      480 
Glu Ile Arg Thr Leu Phe Leu Ser Gln Asp Leu Ile Gly Thr Glu Ala 
145                 150                 155                 160 

cac atg ggc agc agc agc ggc agc agt gcc cac ggt acg ccc tcg att      528 
His Met Gly Ser Ser Ser Gly Ser Ser Ala His Gly Thr Pro Ser Ile 
                165                 170                 175 

ccc gtt gac agc agt gcc cac ggt aca ccc tcg act ccc gtt gac agc      576 
Pro Val Asp Ser Ser Ala His Gly Thr Pro Ser Thr Pro Val Asp Ser 
            180                 185                 190 

agt gcc cac ggt acg ccc tcg act ccc gtt gac agc agt gcc cac ggt      624 
Ser Ala His Gly Thr Pro Ser Thr Pro Val Asp Ser Ser Ala His Gly 
        195                 200                 205 

aca ccc tcg act ccc gtt gac agc agt gcc cac ggt aca ccc tcg act      672 
Thr Pro Ser Thr Pro Val Asp Ser Ser Ala His Gly Thr Pro Ser Thr 
    210                 215                 220 

ccc gtt gac agc agt gcc cac ggt aag ccc tcg act ccc gct gac agc      720 
Pro Val Asp Ser Ser Ala His Gly Lys Pro Ser Thr Pro Ala Asp Ser 
225                 230                 235                 240 

agt gcc cac agt acg ccc tcg act ccc gct gac agc agt gcc cac agt      768 
Ser Ala His Ser Thr Pro Ser Thr Pro Ala Asp Ser Ser Ala His Ser 
                245                 250                 255 

acg ccc tca att ccc gct gac agc agt gcc cac agt acg ccc tca gct      816 
Thr Pro Ser Ile Pro Ala Asp Ser Ser Ala His Ser Thr Pro Ser Ala 
            260                 265                 270 

ccc gct gac aac ggc gcc aat ggt acg gtt ttg att ttg tcg              858 
Pro Ala Asp Asn Gly Ala Asn Gly Thr Val Leu Ile Leu Ser 
        275                 280                 285 

 
           
             44  
             286  
             PRT  
             T CRUZI  
           
            44 

Asp Pro Thr Tyr Arg Phe Ala Asn His Ala Phe Thr Leu Val Ala Ser 
1               5                   10                  15 

Val Thr Ile His Glu Val Pro Ser Val Ala Ser Pro Leu Leu Gly Ala 
            20                  25                  30 

Ser Leu Asp Ser Ser Gly Gly Lys Lys Leu Leu Gly Leu Ser Tyr Asp 
        35                  40                  45 

Glu Lys His Gln Trp Gln Pro Ile Tyr Gly Ser Thr Pro Val Thr Pro 
    50                  55                  60 

Thr Gly Ser Trp Glu Met Gly Lys Arg Tyr His Val Val Leu Thr Met 
65                  70                  75                  80 

Ala Asn Lys Ile Gly Ser Val Tyr Ile Asp Gly Glu Pro Leu Glu Gly 
                85                  90                  95 

Ser Gly Gln Thr Val Val Pro Asp Glu Arg Thr Pro Asp Ile Ser His 
            100                 105                 110 

Phe Tyr Val Gly Gly Tyr Gly Arg Ser Asp Met Pro Thr Ile Ser His 
        115                 120                 125 

Val Thr Val Asn Asn Val Leu Leu Tyr Asn Arg Gln Leu Asn Ala Glu 
    130                 135                 140 

Glu Ile Arg Thr Leu Phe Leu Ser Gln Asp Leu Ile Gly Thr Glu Ala 
145                 150                 155                 160 

His Met Gly Ser Ser Ser Gly Ser Ser Ala His Gly Thr Pro Ser Ile 
                165                 170                 175 

Pro Val Asp Ser Ser Ala His Gly Thr Pro Ser Thr Pro Val Asp Ser 
            180                 185                 190 

Ser Ala His Gly Thr Pro Ser Thr Pro Val Asp Ser Ser Ala His Gly 
        195                 200                 205 

Thr Pro Ser Thr Pro Val Asp Ser Ser Ala His Gly Thr Pro Ser Thr 
    210                 215                 220 

Pro Val Asp Ser Ser Ala His Gly Lys Pro Ser Thr Pro Ala Asp Ser 
225                 230                 235                 240 

Ser Ala His Ser Thr Pro Ser Thr Pro Ala Asp Ser Ser Ala His Ser 
                245                 250                 255 

Thr Pro Ser Ile Pro Ala Asp Ser Ser Ala His Ser Thr Pro Ser Ala 
            260                 265                 270 

Pro Ala Asp Asn Gly Ala Asn Gly Thr Val Leu Ile Leu Ser 
        275                 280                 285 

 
           
             45  
             701  
             DNA  
             T CRUZI  
             
               CDS  
               (1)..(699)  
             
           
            45 

act cat gac gcg tac agg ccc gtt gat ccc tcg gcg tac aag cgc gcc       48 
Thr His Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala 
1               5                   10                  15 

ttg ccg cag gaa gag caa gag gat gtg ggg ccg cgc cac gtt gat ccc       96 
Leu Pro Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro 
            20                  25                  30 

gac cac ttc cgc tcg acc tcg acg act cat gac gcg tac agg ccc gtt      144 
Asp His Phe Arg Ser Thr Ser Thr Thr His Asp Ala Tyr Arg Pro Val 
        35                  40                  45 

gat ccc tcg gcg tac aag cgc gcc ttg ccg cag gaa gag caa gag gat      192 
Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp 
    50                  55                  60 

gtg ggg ccg cgc cac gtt gat ccc gac cac ttc cgc tcg acg act cat      240 
Val Gly Pro Arg His Val Asp Pro Asp His Phe Arg Ser Thr Thr His 
65                  70                  75                  80 

gac gcg tac agg ccc gtt gat ccc tcg gcg tac aag cgc gcc ttg ccg      288 
Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro 
                85                  90                  95 

cag gaa gag caa gag gat gtg ggg ccg cgc cac gtt gat ccc gac cac      336 
Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His 
            100                 105                 110 

ttc cgc tcg acc tcg acg act cat gac gcg tac agg ccc gtt gat ccc      384 
Phe Arg Ser Thr Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro 
        115                 120                 125 

tcg gcg tac aag cgc gcc ttg ccg cag gaa gag caa gag gat gtg ggg      432 
Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly 
    130                 135                 140 

ccg cgc cac gtt gat ccc gac cac ttc cgc tcg acc tcg acg act cat      480 
Pro Arg His Val Asp Pro Asp His Phe Arg Ser Thr Ser Thr Thr His 
145                 150                 155                 160 

gac gcg tac agg ccc gtt gat ccc tcg gcg tac aag cgc gcc ttg ccg      528 
Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro 
                165                 170                 175 

cag gaa gag caa gag gat gtg ggg ccg cgc cac gtt gat ccc gac cac      576 
Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His 
            180                 185                 190 

ttc cgc tcg acg act cat gac gcg tac agg ccc gtt gat ccc tcg gcg      624 
Phe Arg Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala 
        195                 200                 205 

tac aag cgc gcc ttg ccg cag gaa gag caa gag gat gtg ggg ccg cgc      672 
Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly Pro Arg 
    210                 215                 220 

cac gtt gat ccc gac cac ttc cgc tcg ac                               701 
His Val Asp Pro Asp His Phe Arg Ser 
225                 230 

 
           
             46  
             233  
             PRT  
             T CRUZI  
           
            46 

Thr His Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala 
1               5                   10                  15 

Leu Pro Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro 
            20                  25                  30 

Asp His Phe Arg Ser Thr Ser Thr Thr His Asp Ala Tyr Arg Pro Val 
        35                  40                  45 

Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp 
    50                  55                  60 

Val Gly Pro Arg His Val Asp Pro Asp His Phe Arg Ser Thr Thr His 
65                  70                  75                  80 

Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro 
                85                  90                  95 

Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His 
            100                 105                 110 

Phe Arg Ser Thr Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro 
        115                 120                 125 

Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly 
    130                 135                 140 

Pro Arg His Val Asp Pro Asp His Phe Arg Ser Thr Ser Thr Thr His 
145                 150                 155                 160 

Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro 
                165                 170                 175 

Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His 
            180                 185                 190 

Phe Arg Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala 
        195                 200                 205 

Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly Pro Arg 
    210                 215                 220 

His Val Asp Pro Asp His Phe Arg Ser 
225                 230 

 
           
             47  
             1557  
             DNA  
             T CRUZI  
             
               CDS  
               (1)..(1557)  
             
           
            47 

gat cca acg tat cgt ttt gca aac cac gcg ttc acg ctg gtg gcg tcg       48 
Asp Pro Thr Tyr Arg Phe Ala Asn His Ala Phe Thr Leu Val Ala Ser 
1               5                   10                  15 

gtg acg att cac gag gtt ccg agc gtc gcg agt cct ttg ctg ggt gcg       96 
Val Thr Ile His Glu Val Pro Ser Val Ala Ser Pro Leu Leu Gly Ala 
            20                  25                  30 

agc ctg gac tct tct ggt ggc aaa aaa ctc ctg ggg ctc tcg tac gac      144 
Ser Leu Asp Ser Ser Gly Gly Lys Lys Leu Leu Gly Leu Ser Tyr Asp 
        35                  40                  45 

gag aag cac cag tgg cag cca ata tac gga tca acg ccg gtg acg ccg      192 
Glu Lys His Gln Trp Gln Pro Ile Tyr Gly Ser Thr Pro Val Thr Pro 
    50                  55                  60 

acc gga tcg tgg gag atg ggt aag agg tac cac gtg gtt ctt acg atg      240 
Thr Gly Ser Trp Glu Met Gly Lys Arg Tyr His Val Val Leu Thr Met 
65                  70                  75                  80 

gcg aat aaa att ggc tcc gtg tac att gat gga gaa cct ctg gag ggt      288 
Ala Asn Lys Ile Gly Ser Val Tyr Ile Asp Gly Glu Pro Leu Glu Gly 
                85                  90                  95 

tca ggg cag acc gtt gtg cca gac gag agg acg cct gac atc tcc cac      336 
Ser Gly Gln Thr Val Val Pro Asp Glu Arg Thr Pro Asp Ile Ser His 
            100                 105                 110 

ttc tac gtt ggc ggg tat gga agg agt gat atg cca acc ata agc cac      384 
Phe Tyr Val Gly Gly Tyr Gly Arg Ser Asp Met Pro Thr Ile Ser His 
        115                 120                 125 

gtg acg gtg aat aat gtt ctt ctt tac aac cgt cag ctg aat gcc gag      432 
Val Thr Val Asn Asn Val Leu Leu Tyr Asn Arg Gln Leu Asn Ala Glu 
    130                 135                 140 

gag atc agg acc ttg ttc ttg agc cag gac ctg att ggc acg gaa gca      480 
Glu Ile Arg Thr Leu Phe Leu Ser Gln Asp Leu Ile Gly Thr Glu Ala 
145                 150                 155                 160 

cac atg ggc agc agc agc ggc agc agt gcc cac ggt acg ccc tcg att      528 
His Met Gly Ser Ser Ser Gly Ser Ser Ala His Gly Thr Pro Ser Ile 
                165                 170                 175 

ccc gtt gac agc agt gcc cac ggt aca ccc tcg act ccc gtt gac agc      576 
Pro Val Asp Ser Ser Ala His Gly Thr Pro Ser Thr Pro Val Asp Ser 
            180                 185                 190 

agt gcc cac ggt acg ccc tcg act ccc gtt gac agc agt gcc cac ggt      624 
Ser Ala His Gly Thr Pro Ser Thr Pro Val Asp Ser Ser Ala His Gly 
        195                 200                 205 

aca ccc tcg act ccc gtt gac agc agt gcc cac ggt aca ccc tcg act      672 
Thr Pro Ser Thr Pro Val Asp Ser Ser Ala His Gly Thr Pro Ser Thr 
    210                 215                 220 

ccc gtt gac agc agt gcc cac ggt aag ccc tcg act ccc gct gac agc      720 
Pro Val Asp Ser Ser Ala His Gly Lys Pro Ser Thr Pro Ala Asp Ser 
225                 230                 235                 240 

agt gcc cac agt acg ccc tcg act ccc gct gac agc agt gcc cac agt      768 
Ser Ala His Ser Thr Pro Ser Thr Pro Ala Asp Ser Ser Ala His Ser 
                245                 250                 255 

acg ccc tca att ccc gct gac agc agt gcc cac agt acg ccc tca gct      816 
Thr Pro Ser Ile Pro Ala Asp Ser Ser Ala His Ser Thr Pro Ser Ala 
            260                 265                 270 

ccc gct gac aac ggc gcc aat ggt acg gtt ttg att ttg tcg act cat      864 
Pro Ala Asp Asn Gly Ala Asn Gly Thr Val Leu Ile Leu Ser Thr His 
        275                 280                 285 

gac gcg tac agg ccc gtt gat ccc tcg gcg tac aag cgc gcc ttg ccg      912 
Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro 
    290                 295                 300 

cag gaa gag caa gag gat gtg ggg ccg cgc cac gtt gat ccc gac cac      960 
Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His 
305                 310                 315                 320 

ttc cgc tcg acc tcg acg act cat gac gcg tac agg ccc gtt gat ccc     1008 
Phe Arg Ser Thr Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro 
                325                 330                 335 

tcg gcg tac aag cgc gcc ttg ccg cag gaa gag caa gag gat gtg ggg     1056 
Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly 
            340                 345                 350 

ccg cgc cac gtt gat ccc gac cac ttc cgc tcg acg act cat gac gcg     1104 
Pro Arg His Val Asp Pro Asp His Phe Arg Ser Thr Thr His Asp Ala 
        355                 360                 365 

tac agg ccc gtt gat ccc tcg gcg tac aag cgc gcc ttg ccg cag gaa     1152 
Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu 
    370                 375                 380 

gag caa gag gat gtg ggg ccg cgc cac gtt gat ccc gac cac ttc cgc     1200 
Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His Phe Arg 
385                 390                 395                 400 

tcg acc tcg acg act cat gac gcg tac agg ccc gtt gat ccc tcg gcg     1248 
Ser Thr Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala 
                405                 410                 415 

tac aag cgc gcc ttg ccg cag gaa gag caa gag gat gtg ggg ccg cgc     1296 
Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly Pro Arg 
            420                 425                 430 

cac gtt gat ccc gac cac ttc cgc tcg acc tcg acg act cat gac gcg     1344 
His Val Asp Pro Asp His Phe Arg Ser Thr Ser Thr Thr His Asp Ala 
        435                 440                 445 

tac agg ccc gtt gat ccc tcg gcg tac aag cgc gcc ttg ccg cag gaa     1392 
Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu 
    450                 455                 460 

gag caa gag gat gtg ggg ccg cgc cac gtt gat ccc gac cac ttc cgc     1440 
Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His Phe Arg 
465                 470                 475                 480 

tcg acg act cat gac gcg tac agg ccc gtt gat ccc tcg gcg tac aag     1488 
Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys 
                485                 490                 495 

cgc gcc ttg ccg cag gaa gag caa gag gat gtg ggg ccg cgc cac gtt     1536 
Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val 
            500                 505                 510 

gat ccc gac cac ttc cgc tcg                                         1557 
Asp Pro Asp His Phe Arg Ser 
        515 

 
           
             48  
             519  
             PRT  
             T CRUZI  
           
            48 

Asp Pro Thr Tyr Arg Phe Ala Asn His Ala Phe Thr Leu Val Ala Ser 
1               5                   10                  15 

Val Thr Ile His Glu Val Pro Ser Val Ala Ser Pro Leu Leu Gly Ala 
            20                  25                  30 

Ser Leu Asp Ser Ser Gly Gly Lys Lys Leu Leu Gly Leu Ser Tyr Asp 
        35                  40                  45 

Glu Lys His Gln Trp Gln Pro Ile Tyr Gly Ser Thr Pro Val Thr Pro 
    50                  55                  60 

Thr Gly Ser Trp Glu Met Gly Lys Arg Tyr His Val Val Leu Thr Met 
65                  70                  75                  80 

Ala Asn Lys Ile Gly Ser Val Tyr Ile Asp Gly Glu Pro Leu Glu Gly 
                85                  90                  95 

Ser Gly Gln Thr Val Val Pro Asp Glu Arg Thr Pro Asp Ile Ser His 
            100                 105                 110 

Phe Tyr Val Gly Gly Tyr Gly Arg Ser Asp Met Pro Thr Ile Ser His 
        115                 120                 125 

Val Thr Val Asn Asn Val Leu Leu Tyr Asn Arg Gln Leu Asn Ala Glu 
    130                 135                 140 

Glu Ile Arg Thr Leu Phe Leu Ser Gln Asp Leu Ile Gly Thr Glu Ala 
145                 150                 155                 160 

His Met Gly Ser Ser Ser Gly Ser Ser Ala His Gly Thr Pro Ser Ile 
                165                 170                 175 

Pro Val Asp Ser Ser Ala His Gly Thr Pro Ser Thr Pro Val Asp Ser 
            180                 185                 190 

Ser Ala His Gly Thr Pro Ser Thr Pro Val Asp Ser Ser Ala His Gly 
        195                 200                 205 

Thr Pro Ser Thr Pro Val Asp Ser Ser Ala His Gly Thr Pro Ser Thr 
    210                 215                 220 

Pro Val Asp Ser Ser Ala His Gly Lys Pro Ser Thr Pro Ala Asp Ser 
225                 230                 235                 240 

Ser Ala His Ser Thr Pro Ser Thr Pro Ala Asp Ser Ser Ala His Ser 
                245                 250                 255 

Thr Pro Ser Ile Pro Ala Asp Ser Ser Ala His Ser Thr Pro Ser Ala 
            260                 265                 270 

Pro Ala Asp Asn Gly Ala Asn Gly Thr Val Leu Ile Leu Ser Thr His 
        275                 280                 285 

Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro 
    290                 295                 300 

Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His 
305                 310                 315                 320 

Phe Arg Ser Thr Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro 
                325                 330                 335 

Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly 
            340                 345                 350 

Pro Arg His Val Asp Pro Asp His Phe Arg Ser Thr Thr His Asp Ala 
        355                 360                 365 

Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu 
    370                 375                 380 

Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His Phe Arg 
385                 390                 395                 400 

Ser Thr Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala 
                405                 410                 415 

Tyr Lys Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly Pro Arg 
            420                 425                 430 

His Val Asp Pro Asp His Phe Arg Ser Thr Ser Thr Thr His Asp Ala 
        435                 440                 445 

Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys Arg Ala Leu Pro Gln Glu 
    450                 455                 460 

Glu Gln Glu Asp Val Gly Pro Arg His Val Asp Pro Asp His Phe Arg 
465                 470                 475                 480 

Ser Thr Thr His Asp Ala Tyr Arg Pro Val Asp Pro Ser Ala Tyr Lys 
                485                 490                 495 

Arg Ala Leu Pro Gln Glu Glu Gln Glu Asp Val Gly Pro Arg His Val 
            500                 505                 510 

Asp Pro Asp His Phe Arg Ser 
        515 

 
           
             49  
             1521  
             DNA  
             T CRUZI  
             
               CDS  
               (1)..(1521)  
             
           
            49 

atg gcc cga gct gtg gtg ctt gag gat gga gcg ctt tac gtg gcg gac       48 
Met Ala Arg Ala Val Val Leu Glu Asp Gly Ala Leu Tyr Val Ala Asp 
1               5                   10                  15 

aat gcc aac aac ctc gtt cga gaa atc tcc aat ggc gtt gtc act tcg       96 
Asn Ala Asn Asn Leu Val Arg Glu Ile Ser Asn Gly Val Val Thr Ser 
            20                  25                  30 

ttt att acg gaa gga ctg ctg ggc cca tcg tac atc aaa ccg tac agc      144 
Phe Ile Thr Glu Gly Leu Leu Gly Pro Ser Tyr Ile Lys Pro Tyr Ser 
        35                  40                  45 

cgt aca aat ggc gct cat gac ttg ttt gtg tcg gac acg ggc aaa tca      192 
Arg Thr Asn Gly Ala His Asp Leu Phe Val Ser Asp Thr Gly Lys Ser 
    50                  55                  60 

cgc atc att ttt gcc cca cct cag aaa aaa acg ttc atc aca gtg ttt      240 
Arg Ile Ile Phe Ala Pro Pro Gln Lys Lys Thr Phe Ile Thr Val Phe 
65                  70                  75                  80 

ata aca gga ttc cag ccg gat gtt ctt caa att agc gag aag agt cgt      288 
Ile Thr Gly Phe Gln Pro Asp Val Leu Gln Ile Ser Glu Lys Ser Arg 
                85                  90                  95 

ttg atg ttt gcc atc tgc aat tcc acg aaa att ctt gcg att aat atg      336 
Leu Met Phe Ala Ile Cys Asn Ser Thr Lys Ile Leu Ala Ile Asn Met 
            100                 105                 110 

cag gga gcc aca acc ccg aag gag tac tgg caa gtt gga aat gcg gac      384 
Gln Gly Ala Thr Thr Pro Lys Glu Tyr Trp Gln Val Gly Asn Ala Asp 
        115                 120                 125 

tgc atg ggc tat cag agt tcc ctc atg ctc acg acc gag gag gat aaa      432 
Cys Met Gly Tyr Gln Ser Ser Leu Met Leu Thr Thr Glu Glu Asp Lys 
    130                 135                 140 

ctc ctc tac tac ggc ata tta aat gga acc cca tcc atc atg tct tta      480 
Leu Leu Tyr Tyr Gly Ile Leu Asn Gly Thr Pro Ser Ile Met Ser Leu 
145                 150                 155                 160 

ccc gcc acc aaa acg aag acg gaa gca ccc aga att tgc ccg gat gtg      528 
Pro Ala Thr Lys Thr Lys Thr Glu Ala Pro Arg Ile Cys Pro Asp Val 
                165                 170                 175 

ttg ttg cag tgg cca cat ggg ccc att gtt tcg ctt gtg aat att aac      576 
Leu Leu Gln Trp Pro His Gly Pro Ile Val Ser Leu Val Asn Ile Asn 
            180                 185                 190 

aaa cat gca ttt tac gtt gtt acc gcc tcc aat gta tac att gta cat      624 
Lys His Ala Phe Tyr Val Val Thr Ala Ser Asn Val Tyr Ile Val His 
        195                 200                 205 

gat ggc tcg tat cat ccg act gga tcc atg gcc cag ctc caa cag gca      672 
Asp Gly Ser Tyr His Pro Thr Gly Ser Met Ala Gln Leu Gln Gln Ala 
    210                 215                 220 

gaa aat aat atc act aat tcc aaa aaa gaa atg aca aag cta cga gaa      720 
Glu Asn Asn Ile Thr Asn Ser Lys Lys Glu Met Thr Lys Leu Arg Glu 
225                 230                 235                 240 

aaa gtg aaa aag gcc gag aaa gaa aaa ttg gac gcc att aac cgg gca      768 
Lys Val Lys Lys Ala Glu Lys Glu Lys Leu Asp Ala Ile Asn Arg Ala 
                245                 250                 255 

acc aag ctg gaa gag gaa cga aac caa gcg tac aaa gca gca cac aag      816 
Thr Lys Leu Glu Glu Glu Arg Asn Gln Ala Tyr Lys Ala Ala His Lys 
            260                 265                 270 

gca gag gag gaa aag gct aaa aca ttt caa cgc ctt ata aca ttt gag      864 
Ala Glu Glu Glu Lys Ala Lys Thr Phe Gln Arg Leu Ile Thr Phe Glu 
        275                 280                 285 

tcg gaa aat att aac tta aag aaa agg cca aat gac gca gtt tca aat      912 
Ser Glu Asn Ile Asn Leu Lys Lys Arg Pro Asn Asp Ala Val Ser Asn 
    290                 295                 300 

cgg gat aag aaa aaa aat tct gaa acc gca aaa act gac gaa gta gag      960 
Arg Asp Lys Lys Lys Asn Ser Glu Thr Ala Lys Thr Asp Glu Val Glu 
305                 310                 315                 320 

aaa cag agg gcg gct gag gct gcc aag gcc gtg gag acg gag aag cag     1008 
Lys Gln Arg Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln 
                325                 330                 335 

agg gca gct gag gcc acg aag gtt gcc gaa gcg gag aag cgg aag gca     1056 
Arg Ala Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Arg Lys Ala 
            340                 345                 350 

gct gag gcc gcc aag gcc gtg gag acg gag aag cag agg gca gct gaa     1104 
Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu 
        355                 360                 365 

gcc acg aag gtt gcc gaa gcg gag aag cag aag gca gct gag gcc gcc     1152 
Ala Thr Lys Val Ala Glu Ala Glu Lys Gln Lys Ala Ala Glu Ala Ala 
    370                 375                 380 

aag gcc gtg gag acg gag aag cag agg gca gct gaa gcc acg aag gtt     1200 
Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu Ala Thr Lys Val 
385                 390                 395                 400 

gcc gaa gcg gag aag cag agg gca gct gaa gcc atg aag gtt gcc gaa     1248 
Ala Glu Ala Glu Lys Gln Arg Ala Ala Glu Ala Met Lys Val Ala Glu 
                405                 410                 415 

gcg gag aag cag aag gca gct gag gcc gcc aag gcc gtg gag acg gag     1296 
Ala Glu Lys Gln Lys Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu 
            420                 425                 430 

aag cag agg gca gct gaa gcc acg aag gtt gcc gaa gcg gag aag cag     1344 
Lys Gln Arg Ala Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Gln 
        435                 440                 445 

aag gca gct gag gcc gcc aag gcc gtg gag acg gag aag cag agg gca     1392 
Lys Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala 
    450                 455                 460 

gct gaa gcc acg aag gtt gcc gaa gcg gag aag cag aag gca gct gag     1440 
Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Gln Lys Ala Ala Glu 
465                 470                 475                 480 

gcc gcc aag gcc gtg gag acg gag aag cag agg gca gct gaa gcc acg     1488 
Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu Ala Thr 
                485                 490                 495 

aag gtt gcc gaa gcg gag aag gat atc gat ccc                         1521 
Lys Val Ala Glu Ala Glu Lys Asp Ile Asp Pro 
            500                 505 

 
           
             50  
             507  
             PRT  
             T CRUZI  
           
            50 

Met Ala Arg Ala Val Val Leu Glu Asp Gly Ala Leu Tyr Val Ala Asp 
1               5                   10                  15 

Asn Ala Asn Asn Leu Val Arg Glu Ile Ser Asn Gly Val Val Thr Ser 
            20                  25                  30 

Phe Ile Thr Glu Gly Leu Leu Gly Pro Ser Tyr Ile Lys Pro Tyr Ser 
        35                  40                  45 

Arg Thr Asn Gly Ala His Asp Leu Phe Val Ser Asp Thr Gly Lys Ser 
    50                  55                  60 

Arg Ile Ile Phe Ala Pro Pro Gln Lys Lys Thr Phe Ile Thr Val Phe 
65                  70                  75                  80 

Ile Thr Gly Phe Gln Pro Asp Val Leu Gln Ile Ser Glu Lys Ser Arg 
                85                  90                  95 

Leu Met Phe Ala Ile Cys Asn Ser Thr Lys Ile Leu Ala Ile Asn Met 
            100                 105                 110 

Gln Gly Ala Thr Thr Pro Lys Glu Tyr Trp Gln Val Gly Asn Ala Asp 
        115                 120                 125 

Cys Met Gly Tyr Gln Ser Ser Leu Met Leu Thr Thr Glu Glu Asp Lys 
    130                 135                 140 

Leu Leu Tyr Tyr Gly Ile Leu Asn Gly Thr Pro Ser Ile Met Ser Leu 
145                 150                 155                 160 

Pro Ala Thr Lys Thr Lys Thr Glu Ala Pro Arg Ile Cys Pro Asp Val 
                165                 170                 175 

Leu Leu Gln Trp Pro His Gly Pro Ile Val Ser Leu Val Asn Ile Asn 
            180                 185                 190 

Lys His Ala Phe Tyr Val Val Thr Ala Ser Asn Val Tyr Ile Val His 
        195                 200                 205 

Asp Gly Ser Tyr His Pro Thr Gly Ser Met Ala Gln Leu Gln Gln Ala 
    210                 215                 220 

Glu Asn Asn Ile Thr Asn Ser Lys Lys Glu Met Thr Lys Leu Arg Glu 
225                 230                 235                 240 

Lys Val Lys Lys Ala Glu Lys Glu Lys Leu Asp Ala Ile Asn Arg Ala 
                245                 250                 255 

Thr Lys Leu Glu Glu Glu Arg Asn Gln Ala Tyr Lys Ala Ala His Lys 
            260                 265                 270 

Ala Glu Glu Glu Lys Ala Lys Thr Phe Gln Arg Leu Ile Thr Phe Glu 
        275                 280                 285 

Ser Glu Asn Ile Asn Leu Lys Lys Arg Pro Asn Asp Ala Val Ser Asn 
    290                 295                 300 

Arg Asp Lys Lys Lys Asn Ser Glu Thr Ala Lys Thr Asp Glu Val Glu 
305                 310                 315                 320 

Lys Gln Arg Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln 
                325                 330                 335 

Arg Ala Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Arg Lys Ala 
            340                 345                 350 

Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu 
        355                 360                 365 

Ala Thr Lys Val Ala Glu Ala Glu Lys Gln Lys Ala Ala Glu Ala Ala 
    370                 375                 380 

Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu Ala Thr Lys Val 
385                 390                 395                 400 

Ala Glu Ala Glu Lys Gln Arg Ala Ala Glu Ala Met Lys Val Ala Glu 
                405                 410                 415 

Ala Glu Lys Gln Lys Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu 
            420                 425                 430 

Lys Gln Arg Ala Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Gln 
        435                 440                 445 

Lys Ala Ala Glu Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala 
    450                 455                 460 

Ala Glu Ala Thr Lys Val Ala Glu Ala Glu Lys Gln Lys Ala Ala Glu 
465                 470                 475                 480 

Ala Ala Lys Ala Val Glu Thr Glu Lys Gln Arg Ala Ala Glu Ala Thr 
                485                 490                 495 

Lys Val Ala Glu Ala Glu Lys Asp Ile Asp Pro 
            500                 505