Abstract:
The present invention relates to a blood separation container for extracting self-platelet. 
     According to the present invention, since a second coupling portion is formed at a release prevention member coupled to a lower portion of a main body in a PRP separation container, and an ascending member having a bolt structure is screwed to the second coupling portion to ascend a lower cover disposed in a lower fluid chamber of the main body, a separate second main body is unnecessary unlike the related art. Therefore, the PRP separation container is easy to carry, manufacturing costs can be reduced to thereby ensure competitive price, and a PRP can be easily separated and extracted.

Description:
CROSS REFERENCE TO PRIOR APPLICATIONS 
     This application is a continuation application under 35 U.S.C. §365(c) of International Application No. PCT/KR2014/003073 (filed on Apr. 9, 2014) designating the United States, which claims priority to Korean Patent Application Nos. 10-2013-0039895 (filed on Apr. 11, 2013), 20-2013-0007002 (filed on Aug. 21, 2013) and 20-2013-0008820 (filed on Oct. 28, 2013), which are all hereby incorporated by reference in their entireties. 
    
    
     TECHNICAL FIELD 
     The present invention relates to a blood separation container for extracting self-platelet. 
     BACKGROUND ART 
     Generally, blood is used as an important index for determining various diseases or health conditions. Platelets abundantly containing growth factors in the blood are used for therapeutic purposes. Blood is composed of red blood cells, white blood cells, and platelets, and the platelets mainly exist in a plasma. The plasma is divided into a platelet rich plasma (PRP) and a platelet poor plasma (PPP). The PRP is transplanted into a pain area, in particular, knees, ligaments, muscles, or the like, and stimulates stem cells to help in creating cells. As such, the PRP has been used for therapeutic purposes. 
     Since the PRP is a small amount of about 1% in a collected blood and its viscosity is high, it is difficult to separate the PRP from red blood cells. Therefore, research has been actively conducted on technologies for extracting a PRP other than red blood cells. Generally, after blood collected from a human body is injected into a PRP separation container, the PRP is extracted using a centrifuge. In the past, a test tube has been commonly used as the PRP separation container. However, since the PRP extraction is inconvenient, a separation container with a fluid chamber divided into two chambers has recently been developed and used. 
     An example of the PRP separation container has been proposed in Korean Patent Registration No. 10-1128163 (registration date: Mar. 12, 2012) by the inventor of the present application and is illustrated in  FIG. 1 . 
       FIG. 1  is a perspective view for describing a PRP separation container according to the related art. 
     As illustrated in  FIG. 1 , the PRP separation container  10  according to the related art includes: a main body  11  defining an internal space divided into an upper fluid chamber  12  and a lower fluid chamber  13 ; an upper cover  15  disposed to seal an upper portion of the upper fluid chamber  12 ; a lower cover  16  disposed to seal a lower portion of the lower fluid chamber  13  and coupled to be ascendable in such a state that an outer peripheral surface of the lower cover  16  comes into close contact with an inner peripheral surface of the lower fluid chamber  13  by an external force applied upward; and a fluid collection part  14  dividing the internal space into the upper fluid chamber  12  and the lower fluid chamber  13  and including a fluid passage protruding upward from a central portion to communicate the upper fluid chamber  12  with the lower fluid chamber  13 . 
     A PRP extracting method using the PRP separation container  10  will be described below. 
     First, blood collected from a human body is injected into the lower fluid chamber  13  by using a syringe, and a centrifugation is performed on the PRP separation container  10 , into which the blood is injected, by using a centrifuge. 
     When the centrifugation is completed, the blood is separated into a red blood cell, a PRP, and a PPP in this order from the lower portion of the lower fluid chamber  13  due to a specific gravity difference. Next, the PPP partially accommodated in the lower fluid chamber  13  is introduced into the upper fluid chamber  12  through a fluid passage  14   a  by ascending the lower cover  16 . 
     After that, plasma accommodated in the lower fluid chamber  13  is introduced into the upper fluid chamber  12  through the fluid passage  14   a  by continuously ascending the lower cover  16 . In a state in which the PRP exists in the fluid passage  14   a , a syringe is inserted into the fluid passage  14   a  through an injection port of the upper cover  15 , and the PRP existing in the fluid passage  14   a  is extracted. In this way, the PRP can be easily separated and extracted. 
     However, in order to ascend the lower cover disposed inside the PRP separation container, the PRP separation container according to the related art, which is proposed in Korean Patent Registration No. 10-1128163, has necessarily need the main body having a relatively complex structure. Accordingly, since it is necessary to separately manufacture the main body, a manufacturing cost of the main body increases and thus a manufacturing cost of the PRP separation container increases. This undermines price competitiveness and the PRP separation container is difficult to carry. 
     On the other hand, recently, fibrin has been extracted by separating PRP and PPP using the above-described PRP separation container and then solidifying the separated PRP and PPP. The fibrin has been used as a base of an implant for a dental treatment, and the demand for the fibrin has been steadily increased. If the demand for the fibrin extends to an orthopedic area, the demand for the fibrin is expected to explosively increase. 
     When the PRP and the PPP are exposed to air, the PRP and the PPP begin to naturally coagulate. However, since a long time is taken until the PRP and the PPP coagulate, a reduction in a coagulation time is most important in terms of productivity. Therefore, in a state in which the PRP and PPP remains in the upper fluid chamber of the PRP separation container, in which the PRP and the PPP are separated from each other, the PRP and the PPP are coagulated by using the centrifuge again. In this manner, the coagulation time of the PRP and the PPP has been reduced to some extent. 
     Although the method of coagulating the PRP and the PPP using the centrifuge as in the related art can reduce the coagulation time of the PRP and PPP to some extent, about 20 minutes to about 30 minutes are taken until the PRP and the PPP coagulate, resulting in a reduction in productivity. Therefore, there is a need for research and development of technologies capable of reducing the coagulation time. 
     CITATION LIST 
     Patent Literature 
     KR 10-1026599 B1, 2011. Mar. 25. pp. 3-6 
     KR 10-2011-0009651 A, 2011. Jan. 28. pp. 3-7 
     KR 10-1128163 B1, 2012. Mar. 12. pp. 6-8, FIGS. 1 to 5 
     SUMMARY OF INVENTION 
     Technical Problem 
     Therefore, the present invention has been made in an effort to solve the problems of the related art and have the following objects. 
     First, an object of the present invention is to provide a blood separation container for extracting self-platelet, which is capable of easily separating and extracting a PRP while reducing manufacturing costs to thereby ensure competitive price. 
     Second, another object of the present invention is to provide a blood separation container for extracting self-platelet, which is capable of rapidly coagulating a PRP and a PPP separated from blood to thereby improve extraction productivity of fibrin. 
     Third, another object of the present invention is to provide a blood separation container for extracting self-platelet, which is capable of improving a collect rate of a buffy coat. 
     Solution to Problem 
     According to an aspect for achieving the above objects, a blood separation container includes: a main body defining an inner space divided into an upper fluid chamber and a lower fluid chamber; an upper cover sealing the upper fluid chamber; a lower cover ascending along an inner peripheral surface of the lower fluid chamber in such a state that an outer peripheral surface of the lower cover comes into close contact with the inner peripheral surface of the lower fluid chamber; a fluid collection part which is formed between the upper fluid chamber and the lower fluid chamber and includes a fluid passage protruding upward from a central portion to communicate the upper fluid chamber and the lower fluid chamber; a release prevention member which is coupled to an outer peripheral surface of the main body and includes a first coupling portion and a second coupling portion that communicates with the first coupling portion, a thread being formed on an inner peripheral surface of the first coupling portion so as to be coupled to a thread formed on a lower outer peripheral surface of the main body, and a thread being formed on an inner peripheral surface of the second coupling portion so as to have a diameter less than a diameter of the first coupling portion; and an ascending member which is inserted into and coupled to the second coupling portion of the release prevention member, a portion of which is screwed to the second coupling portion of the release prevention member in a bolt structure, and which is carried in the lower fluid chamber of the main body and varies in a variation in a volume of the lower fluid chamber by ascending the lower cover coming into close contact with the inner peripheral surface of the lower fluid chamber. 
     According to another aspect for achieving the above objects, a blood separation container includes: a body defining an inner space divided into an upper fluid chamber and a lower fluid chamber; an upper cover sealing the upper fluid chamber; a lower cover ascending along an inner peripheral surface of the lower fluid chamber in such a state that an outer peripheral surface of the lower cover comes into close contact with the inner peripheral surface of the lower fluid chamber; and a fluid collection part which is formed in a funnel shape and includes a fluid passage communicating the lower fluid chamber and the upper fluid chamber, the funnel shape having a mountain-shaped structure that extends from an inner sidewall of the main body to a central portion of the main body at a boundary between the upper fluid chamber and the lower fluid chamber and protrudes toward the upper fluid chamber from the inner sidewall to the central portion, wherein the fluid passage is formed such that an angle of an inlet side in the fluid passage, which is opposed to the lower fluid chamber, is in the range of 70° to 85°. 
     Preferably, the blood separation container may further include a release prevention member coupled to a lower outer peripheral surface of the main body, and the release prevention member may include a first coupling portion and a second coupling portion that communicates with the first coupling portion, a thread being formed on an inner peripheral surface of the first coupling portion so as to be coupled to a thread formed on a lower outer peripheral surface of the main body, and a thread being formed on an inner peripheral surface of the second coupling portion so as to have a diameter less than a diameter of the first coupling portion. 
     Preferably, the blood separation container may further include an ascending member inserted into and coupled to the second coupling portion, and a portion of the ascending member may be screwed to the second coupling portion of the release prevention member in a bolt structure, and the ascending member may be carried in the lower fluid chamber of the main body and vary in a variation in a volume of the lower fluid chamber by ascending the lower cover coming into close contact with the inner peripheral surface of the lower fluid chamber. 
     In addition, according to another aspect for achieving the above objects, a blood separation container includes: a body defining an inner space divided into an upper fluid chamber and a lower fluid chamber; an upper cover sealing the upper fluid chamber; a lower cover ascending along an inner peripheral surface of the lower fluid chamber in such a state that an outer peripheral surface of the lower cover comes into close contact with the inner peripheral surface of the lower fluid chamber; a fluid collection part which is formed between the upper fluid chamber and the lower fluid chamber and includes a fluid passage protruding upward from a central portion to communicate the upper fluid chamber and the lower fluid chamber; and a coagulation catalyst body made of a circular tube including a glass material and inserted into the upper fluid chamber or the lower fluid chamber to function as a catalyst such that a platelet rich plasma (PRP) and a platelet poor plasma (PPP) rapidly coagulate at the time of coagulating the PRP and the PPP by using a centrifuge. 
     Preferably, the blood separation container may further include a release prevention member coupled to a lower outer peripheral surface of the main body, and the release prevention member may include a first coupling portion and a second coupling portion that communicates with the first coupling portion, a thread being formed on an inner peripheral surface of the first coupling portion so as to be coupled to a thread formed on a lower outer peripheral surface of the main body, and a thread being formed on an inner peripheral surface of the second coupling portion so as to have a diameter less than a diameter of the first coupling portion. 
     Preferably, the blood separation container may further include an ascending member inserted into and coupled to the second coupling portion, and wherein a portion of the ascending member may be screwed to the second coupling portion of the release prevention member in a bolt structure, and the ascending member may be carried in the lower fluid chamber of the main body and vary in a variation in a volume of the lower fluid chamber by ascending the lower cover coming into close contact with the inner peripheral surface of the lower fluid chamber. 
     Advantageous Effects of Invention 
     As described above, according to the present invention, the following effects can be obtained. 
     First, according to the present invention, since a second coupling portion is formed at a release prevention member coupled to a lower portion of a main body in a PRP separation container, and an ascending member having a bolt structure is screwed to the second coupling portion to ascend a lower cover disposed in a lower fluid chamber of the main body, a separate second main body is unnecessary unlike the related art. Therefore, the PRP separation container is easy to carry, manufacturing costs can be reduced to thereby ensure competitive price, and a PRP can be easily separated and extracted. 
     Second, according to the present invention, since a secondary centrifugation is performed in a state in which a coagulation catalyst body made of a glass material is inserted into an upper fluid chamber or a lower fluid chamber of the PRP separation container, a coagulation time of a PRP and a PPP can be significantly reduced, thereby improving extraction productivity of fibrin. 
     Third, according to the present invention, by providing the PRP separation container including a buffy coat extraction tube, which extracts a buffy coat generated after a centrifugation and of which an inlet has an angle range of 70° to 80°, a collect rate of the buffy coat can be increased by minimizing a loss of a portion of the buffy coat due to the adhering of the buffy coat to an inner surface of the buffy coat extraction tube during extracting of the buffy coat after the centrifugation. 
    
    
     
       BRIEF DESCRIPTION OF DRAWINGS 
         FIG. 1  is a perspective view of a PRP separation container according to the related art. 
         FIG. 2  is an assembly perspective view of a blood separation container according to an embodiment of the present invention. 
         FIG. 3  is an exploded perspective view of the blood separation container illustrated in  FIG. 2 . 
         FIG. 4  is a cross-sectional perspective view of the blood separation container illustrated in  FIG. 2 . 
         FIG. 5  is a front cross-sectional view of the blood separation container illustrated in  FIG. 2 . 
         FIG. 6  is a flowchart of a PRP and PPP separating and coagulating method according to an embodiment of the present invention. 
     
    
    
     DESCRIPTION OF REFERENCE NUMERALS 
     
         
         
           
               20 : blood separation container 
               21 : main body 
               22 : upper fluid chamber 
               23 : lower fluid chamber 
               24 : fluid collection part 
               24   a : fluid passage 
               25 : upper cover 
               26 : lower cover 
               27 : coagulation catalyst body 
               28 : reinforcement member 
               29 : release prevention member 
               30 : packing member 
               31 : fixing pin 
               32 : ascending member 
           
         
       
    
     DESCRIPTION OF EMBODIMENTS 
     Hereinafter, technical features of the present invention will be described in detail with reference to the accompanying drawings. 
       FIG. 2  is an assembly perspective view of a blood separation container according to an embodiment of the present invention,  FIG. 3  is an exploded perspective view of the blood separation container illustrated in  FIG. 2 ,  FIG. 4  is a cross-sectional perspective view of the blood separation container illustrated in  FIG. 2 , and  FIG. 5  is a front cross-sectional view of the blood separation container illustrated in  FIG. 2 . 
     Referring to  FIGS. 2 to 5 , the blood separation container  20  according to the embodiment of the present invention includes a main body  21  defining an internal space divided into an upper fluid chamber  22  and a lower fluid chamber  23 . 
     The main body  21  has a cylindrical structure and has an opened upper portion and an opened lower portion. The main body  21  may be made of a transparent material or a translucent material that allows a user to see the internal space. 
     The lower fluid chamber  23  of the main body  21  may have a diameter less than a diameter of the upper fluid chamber  22  such that a centrifugation is performed in a state in which the main body  21  is stably accommodated inside a centrifuge. Therefore, a stepped portion is formed at a boundary portion between the upper fluid chamber  22  and the lower fluid chamber  23 . 
     When the main body  21  is accommodated in an accommodation unit of the centrifuge, the stepped portion formed at the boundary portion between the upper fluid chamber  22  and the lower fluid chamber  23  in the main body  21  may be locked to an entrance of the accommodation unit, and accordingly, a lower portion of the main body  21  may be spaced apart from a bottom surface of the accommodation unit in the centrifuge, thereby preventing the lower portion of the main body  21  from colliding with the bottom portion of the accommodation unit in the centrifuge and preventing the lower portion of the main body  21  from being damaged. 
     On the other hand, as illustrated in  FIGS. 2 to 5 , the main body  21  according to the present invention is formed to have a structure in which the upper fluid chamber  22  and the lower fluid chamber  23  have different diameters, but the structure is merely exemplary and the main body  21  according to the present invention is not limited to the structure illustrated in  FIGS. 2 to 5 . For example, the upper fluid chamber  22  and the lower fluid chamber  23  may be formed to have the same diameter. 
     In addition, the blood separation container  20  according to the embodiment of the present invention includes an upper cover  25  coupled to seal an upper opening of the main body  21 , that is, the upper fluid chamber  12 . To this end, in order to seal an opened portion of the upper fluid chamber  22 , a thread  21   a  (see  FIG. 3 ), which is coupled to a thread  25   a  (see  FIG. 5 ) formed on an inner peripheral surface of the upper cover  25 , is formed on an upper outer peripheral surface of the main body  21 . 
     As illustrated in  FIG. 3 , an injection port  25   b  is provided at a central portion of the upper cover  25  so as to inject blood collected from a human body into a fluid passage  24   a  or extract a PRP, fibrin, a buffy coat, or the like separated inside the main body  21 . It is preferable that the injection port  25   b  is sealed with a packing member  30  made of a soft material into which a needle of a syringe is inserted. 
     As illustrated in  FIG. 3 , in order to prevent the packing member  30  from being separated from the upper cover  25  at the time of opening the injection port  25   b , it is preferable that the packing member  30  is disposed to be fixed to an upper portion of the upper cover  25  by a fixing pin  31 . That is, since one side portion of the packing member  30  is disposed to be fixed to the upper cover  25  by the fixing pin  31  that passes through a fixing groove  30   a  defined in the one side portion of the packing member  30  and is inserted into a coupling groove  25   c  defined in the upper cover  25 , a portion of the packing member  30  is fixed to the upper cover  25  to prevent the packing member  30  from being separated from the upper cover  25  even at the time of opening the injection port  25   b , thereby reducing a risk of loss. 
     In addition, as illustrated in  FIGS. 4 and 5 , the blood separation container  20  according to the present invention includes a lower cover  26  that ascends along an inner peripheral surface of the lower fluid chamber  23  in a state in which an outer peripheral surface of the lower cover  26  comes into close contact with the inner peripheral surface of the lower fluid chamber  23  such that blood injected into the lower fluid chamber  23  does leak toward a lower opening of the main body  21 . 
     The lower cover  26  is inserted through the lower opening of the main body  21  to seal the lower fluid chamber  23  and simultaneously, ascends toward a fluid collection part  24  inside the lower fluid chamber  23  in the state in which the outer peripheral surface of the lower cover  26  comes into close contact with the inner peripheral surface of the lower fluid chamber  23  when a strong pressure is applied upward. 
     In addition, as illustrated in  FIGS. 2 and 5 , the blood separation container  20  according to the present invention includes the fluid collection part  24  that is formed between the upper fluid chamber  22  and the lower fluid chamber  23  and includes the fluid passage  24   a  protruding upward from a central portion to communicate the upper fluid chamber  22  and the lower fluid chamber  23 . 
     The fluid collection part  24  is formed to have a mountain-shaped structure protruding upward from an outer portion toward a central portion. The fluid passage  24   a  of the fluid collection part  24  may be disposed spaced a predetermined distance apart from an inner surface (ceiling surface) of the upper cover  25 , a lower portion of the fluid collection part  24  communicates with the lower fluid chamber  23 , and a top portion of the fluid collection part  24  communicates with the upper fluid chamber  22 . 
     As illustrated in  FIG. 5 , the fluid passage  24   a  is formed such that an angle (θ) of an inlet side is in the range of 70° to 85°, preferably 80°, toward the lower fluid chamber  23 . This is because a collect rate of the buffy coat is increased by minimizing a loss of a portion of the buffy coat due to the adhering of the buffy coat to an inner surface of the fluid passage  24   a  during extracting of the buffy coat through the fluid passage  24   a . Actually, when the angle of the inlet side in the fluid passage  24   a  was less than 70°, the collect rate of the buffy coat was significantly decreased. 
     As illustrated in  FIG. 3 , the blood separation container  20  according to the present invention may include a coagulation catalyst body  27  that has a cylindrical structure and is inserted into the upper fluid chamber  22  to function as a catalyst such that a PRP and a PPP rapidly coagulate at the time of coagulating the PRP and the PPP by using the centrifuge. 
     The coagulation catalyst body  27  may be made of a glass material and may be disposed such that an outer peripheral surface of the coagulation catalyst body  27  comes into contact with or is spaced a predetermined distance apart from the inner peripheral surface of the upper fluid chamber  22 . Preferably, in order to prevent the coagulation catalyst body  27  from being damaged due to independent rotation inside the upper fluid chamber  22  during the centrifugation using the centrifuge, the coagulation catalyst body  27  is disposed such that the outer peripheral surface of the catalyst body  27  comes into contact with the inner peripheral surface of the upper fluid chamber  22 . 
     On the other hand, although not illustrated, the coagulation catalyst body  27  according to the present invention may be disposed in the lower fluid chamber  23  instead of the upper fluid chamber  22 . 
     As illustrated in  FIG. 3 , the blood separation container  20  according to the present invention may further include a reinforcement member  28  (see  FIG. 3 ) coupled to a lower surface of the lower cover  26  so as to support the lower cover  26  from an external force applied to a lower portion of the lower cover  26  by an ascending member  32 . 
     In addition, the blood separation container  20  according to the present invention includes a release prevention member  29  screwed to a lower portion of the main body  21  so as to prevent the lower cover  26  from being released from the main body  21  due to a strong rotational force during the centrifugation using the centrifuge. 
     As illustrated in  FIGS. 2 to 4 , the release prevention member  29  includes a first coupling portion  29   a  and a second coupling portion  29   b  that communicates with the first coupling portion  29   a  so as to be coupled, in a bolt structure, to the ascending member  32 , on an outer peripheral surface of which a thread  32 - 2   a  is formed, a thread  29   a - 1  being formed on an inner peripheral surface of the first coupling portion  29   a  so as to be coupled to a thread  21   b  formed on the outer peripheral surface of the main body  21 , and a thread  29   b - 1  being formed on an inner peripheral surface of the second coupling portion  29   b . At this time, the second coupling portion  29   b  is formed to have a diameter less than a diameter of the first coupling portion  29   a  so as not to come into contact with the first coupling portion  29   a  when the ascending member  32  is screwed to penetrate the second coupling portion  29   b.    
     As illustrated in  FIGS. 3 and 4 , the ascending member has a bolt structure. Specifically, the ascending member  32  includes an operation unit  32 - 1  that is operable with a user hand and a coupling unit  32 - 2  that has one side portion integrally coupled to the operation unit  32 - 1  and the other side portion inserted into the second coupling portion  29   b  of the release prevention member  29  and is coupled to the second coupling portion  29   b  of the release prevention member  29  through a male thread formed on an outer peripheral surface of the coupling unit  32 - 2 . 
     The thread  32 - 2   a  is formed on an outer peripheral surface of a region of the ascending member  32  such that a portion of the coupling unit  32 - 2  is coupled to the thread  29   b - 1  formed on the inner peripheral surface of the second coupling portion  29   b  (see  FIG. 3 ) in the release prevention member  29 , the region of the ascending member  32  being inserted into the second coupling portion  29   b . Therefore, the ascending member  32  is inserted into and screwed to the second coupling portion  29   b  of the release prevention member  29 , is coupled to the second coupling portion  29   b  so as to be elevatable, and ascends the lower cover  26  disposed in the lower fluid chamber  23  through an ascending operation to vary a variation in a volume of the lower fluid chamber  23 . 
       FIG. 6  is a flowchart of a PRP and PPP separating and coagulating method according to an embodiment of the present invention. 
     First, referring to  FIGS. 2 to 6 , a blood separation container  20  is prepared (ST 1 ). 
     The preparing of the blood separation container  20  is performed by coupling an upper cover  25  to an upper portion of a main body  21  to seal an upper portion of the upper fluid chamber  22  in a state in which a coagulation catalyst body  27  is inserted into an upper inside of the main body  21 , that is, the upper fluid chamber  22  (or a lower fluid chamber  23 ), and inserting a lower cover  26  to a lower inside of the main body  21 , that is, the lower fluid chamber  23  by press fit to seal a lower portion of the lower fluid chamber  23 . A release prevention member  29  may be coupled to an outer surface of the main body  21 . 
     Next, a sterilization treatment is performed on the blood separation container  20  in the state in which the coagulation catalyst body  27  is inserted into the upper fluid chamber  22  (ST 2 ). 
     Next, blood collected from a human body through an injection port  25   b  of an upper cover  25  is injected into the main body  21 , that is, the lower fluid chamber  23  through a fluid passage  24   a  of a fluid collection part  24  (ST 3 ). 
     Next, after the blood separation container  20 , in which the blood is injected into the lower fluid chamber  23 , is accommodated in a centrifuge, a primary centrifugation is performed on the blood to separate a red blood cell, a PRP, and a PPP inside the lower fluid chamber  23  (ST 4 ). 
     Next, the blood separation container  20 , in which the primary centrifugation is completed, is carried out from the centrifuge, and then, an ascending member  32  is inserted into the lower fluid chamber  23  through a second coupling portion  29   b  of a release prevention member  29 . Next, among the blood cell, the PRP, and the PPP inside the lower fluid chamber  23 , the PRP and the PPP except for the red blood cell are introduced into the upper fluid chamber  22  through the fluid passage  24   a  of the fluid collection part  24  to separate the PRP and the PPP from the red blood cell by rotating the ascending member  32  in a clockwise direction to ascend the lower cover  26  along an inner peripheral surface of the lower fluid chamber  23  (ST 5 ). 
     After that, in a state in which the PRP and the PPP are introduced into the upper fluid chamber  22  and are separated from the red blood cell, after the ascending member  32  is separated from the release prevention member  29 , the blood separation container  20  is accommodated in the centrifuge to perform a secondary centrifugation (ST 6 ). The processes are performed for 3 minutes to 5 minutes to coagulate the PRP and the PPP introduced into the upper fluid chamber  22  to thereby extract fibrin (or a buffy coat). 
     Since the secondary centrifugation performed in step ST 6  is performed in the state in which the coagulation catalyst body  27  made of a glass material is inserted into the upper fluid chamber  22 , a coagulation time of the PRP and the PPP is reduced, as compared with a case where the secondary centrifugation is performed in the absence of the coagulation catalyst body  27 . 
     In order to confirm the coagulation rate of the PRP and the PPP through the secondary centrifugation, the secondary centrifugation was performed in the state in which the coagulation catalyst body  27  was inserted into the upper fluid chamber  22 , and as a result, about 3 minutes to about 5 minutes were taken until the PRP and PPP coagulated. On the contrary, when the secondary coagulation was performed in a state in which the coagulation catalyst body according to the present invention was not inserted into the upper fluid chamber  22 , more time was taken until the PRP and PPP are coagulated. 
     As described above, according to the present invention, since the secondary coagulation is performed in the state in which the coagulation catalyst body  27  is inserted into the upper fluid chamber  22 , the coagulation time of the PRP and PPP may be significantly reduced, thereby improving extraction productivity of the fibrin (or the buffy coat). 
     While the present invention has been shown and described in connection with the exemplary embodiments, it will be apparent to those skilled in the art that modifications and variations can be made without departing from the spirit and scope of the invention as defined by the appended claims.