Abstract:
The present invention relates to compositions and methods for enhancing the effect of vaccines in animals, such as domestic, sport, or pet species, and humans. More particularly, the use of Ribavirin as an adjuvant to a vaccine protocol and compositions having Ribavirin and an antigen are described.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application is a continuation of U.S. application Ser. No. 09/705,547 having a filing date of Nov. 3, 2000, which claims the benefit of priority of U.S. provisional patent application No. 60/229,175, filed Aug. 29, 2000; both of which are hereby expressly incorporated by reference in their entireties. 
    
    
     FIELD OF THE INVENTION 
     The present invention relates to compositions and methods for enhancing the effect of vaccines in animals, such as domestic, sport, or pet species, and humans. More particularly, preferred embodiments concern the use of Ribavirin as an adjuvant and compositions having Ribavirin and an antigen. 
     BACKGROUND OF THE INVENTION 
     The use of vaccines to prevent disease in humans, farm livestock, sports animals, and household pets is a common practice. Frequently, however, the antigen used in a vaccine is not sufficiently immunogenic to raise the antibody titre to levels that are sufficient to provide protection against subsequent challenge or to maintain the potential for mounting these levels over extended time periods. Further, many vaccines are altogether deficient in inducing cell-mediated immunity, which is a primary immune defense against bacterial and viral infection. A considerable amount of research is currently focussed on the development of more potent vaccines and ways to enhance the immunogenicity of antigen-containing preparations. (See e.g., U.S. Pat. Nos. 6,056,961; 6,060,068; 6,063,380; and Li et al.,  Science  288:2219-2222 (2000)). 
     Notorious among such “weak” vaccines are hepatitis B vaccines. For example, recombinant vaccines against hepatitis B virus such as Genhevacb (Pasteur Merieux Serums et Vaccines, 58, Avenue Leclerc 69007 Lyon, France), Engerixb (Smith, Kline and Symbol French), and Recombivaxhb (Merck, Sharp, and Dhome) are effective only after at least three injections at 0, 30, and 60 or 180 days, followed by an obligatory booster after one year. (Chedid et al., U.S. Pat. No. 6,063,380). Additionally, many subjects receiving these vaccines respond poorly, if at all. Because many regions of the world are endemic for HBV infection, the poorly immunogenic character of existing HBV vaccines has become an extremely serious problem. 
     To obtain a stronger, humoral and/or cellular response, it is common to administer a vaccine in a material that enhances the immune response of the patient to the antigen present in the vaccine. The most commonly used adjuvants for vaccine protocols are oil preparations and alum. (Chedid et al., U.S. Pat. No. 6,063,380). A greater repertoire of safe and effective adjuvants is needed. 
     Nucleoside analogs have been widely used in anti-viral therapies due to their capacity to reduce viral replication. (Hosoya et al.,  J. Inf. Dis ., 168:641-646 (1993)). Ribavirin (1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide) is a synthetic guanosine analog that has been used to inhibit RNA and DNA virus replication. (Huffman et al.,  Antimicrob. Agents. Chemother ., 3:235 (1973); Sidwell et al.,  Science , 177:705 (1972)). Ribavirin has been shown to be a competitive inhibitor of inositol mono-phosphate (IMP) dehydrogenase (IMPDH), which converts IMP to IMX (which is then converted to GMP). De Clercq,  Anti viral Agents: characteristic activity spectrum depending on the molecular target with which they interact , Academic press, Inc., New York N.Y., pp. 1-55 (1993). Intracellular pools of GTP become depleted as a result of long term Ribavirin treatment. 
     In addition to antiviral activity, investigators have observed that a few guanosine analogs have an effect on the immune system. (U.S. Pat. Nos. 6,063,772 and 4,950,647). Ribavirin has been shown to inhibit functional humoral immune responses (Peavy et al.,  J. Immunol ., 126:861-864 (1981); Powers et al.,  Antimicrob. Agents. Chemother ., 22:108-114 (1982)) and IgE-mediated modulation of mast cell secretion. (Marquardt et al.,  J. Pharmacol. Exp. Therapeutics , 240:145-149 (1987)). Some investigators report that a daily oral therapy of Ribavirin has an immune modulating effect on humans and mice. (Hultgren et al.,  J. Gen. Virol ., 79:2381-2391 (1998) and Cramp et al.,  Gastron. Enterol ., 118:346-355 (2000)). Nevertheless, the current understanding of the effects of Ribavirin on the immune system is in its infancy. 
     SUMMARY OF THE INVENTION 
     It has been discovered that Ribavirin can be used as an adjuvant to enhance an immune response to an antigen. Embodiments described herein include “strong” vaccine preparations that comprise an antigen and Ribavirin. Generally, these preparations have an amount of Ribavirin that is sufficient to enhance an immune response to the antigen. Other aspects of the invention include methods of enhancing the immune response of an animal, including a human, to an antigen. By one approach, for example, an animal in need of a potent immune response to an antigen is identified and then is provided an amount of Ribavirin together with the antigen that is effective to enhance an immune response in the animal. In some methods, the Ribavirin and the antigen are provided in combination and in others, the Ribavirin and the antigen are provided separately. Thus, several embodiments concern the manufacture and use of vaccine preparations having Ribavirin and an antigen. 
     Preferred vaccine compositions comprise Ribavirin and a hepatitis viral antigen. The antigen can be a peptide or nucleic acid-based (e.g., a RNA encoding a peptide antigen or a construct that expresses a peptide antigen when introduced to a subject). HBV antigens that are suitable include, for example, hepatitis B surface antigen (HBsAg), hepatitis core antigen (HBcAg), hepatitis e antigen (HBeAg), and nucleic acids encoding these molecules. Compositions having Ribavirin and an antigen from the hepatitis A virus (HAV) or Ribavirin and a nucleic acid encoding an antigen from HAV are also embodiments. Still further, compositions having Ribavirin and an antigen from the hepatitis C virus (HCV) or Ribavirin and a nucleic acid encoding an antigen from HCV are embodiments. 
     Furthermore, compositions having a mixture of the antigens above are embodiments of the present invention. For example, some compositions comprise a HBV antigen, a HAV antigen, and Ribavirin or a HBV antigen, a HCV antigen, and Ribavirin or a HAV antigen, a HCV antigen, and Ribavirin or a HBV antigen, a HAV antigen, a HCV antigen, and Ribavirin. Other embodiments comprise Ribavirin and a nucleic acid encoding a mixture of the antigens described above. Some embodiments also include other adjuvants, binders, emulsifiers, carriers, and fillers, as known in the art, including, but not limited to, alum, oil, and other compounds that enhance an immune response. 
     Preferred methods involve providing an animal in need with a sufficient amount of Ribavirin and a hepatitis viral antigen (e.g., HBV antigen, HAV antigen, HCV antigen a nucleic acid encoding one of these antigens or any combination thereof). Accordingly, one embodiment includes identifying an animal in need of an enhanced immune response to a hepatitis viral antigen (e.g., an animal at risk or already infected with a hepatitis infection) and providing to said animal an amount of Ribavirin that is effective to enhance an immune response to the hepatitis viral antigen. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 is a graph showing the humoral response to 10 and 100 μg recombinant Hepatitis C virus (HCV) non structural 3 protein (NS3), as determined by mean end point titres, when a single dose of 1 mg of Ribavirin was co-administered. 
     FIG. 2 is a graph showing the humoral response to 20 μg recombinant Hepatitis C virus (HCV) non structural 3 protein (NS3), as determined by mean end point titres, when a single dose of 0.1, 1.0, or 10 mg of Ribavirin was co-administered. 
     FIG. 3 is a graph showing the effects of a single dose of 1 mg Ribavirin on NS3-specific lymph node proliferative responses, as determined by in vitro recall responses. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     It has been discovered that compositions comprising Ribavirin and an antigen can boost an animal&#39;s immune response to the antigen. That is, Ribavirin can be used as an “adjuvant,” which for the purposes of this disclosure, refers to a compound that has the ability to enhance the immune response to a particular antigen. Such adjuvant activity is manifested by a significant increase in immune-mediated protection against the antigen, and was demonstrated by an increase in the titer of antibody raised to the antigen and an increase in proliferative T cell responses. 
     Several vaccine preparations that comprise Ribavirin and an antigen are described herein. Vaccine formulations containing Ribavirin can vary according to the amount of Ribavirin, the form of Ribavirin, and the type of antigen. The antigen can be a peptide or a nucleic acid (e.g., a RNA encoding a peptide antigen or a construct that expresses a peptide antigen when introduced into a subject). Preferred vaccine formulations comprise Ribavirin and a hepatitis viral antigen (e.g., HBV antigen, HAV antigen, HCV antigen, a nucleic acid encoding these molecules, or any combination thereof). 
     Methods of enhancing the immune response of an animal, including humans, to an antigen are also described herein. One method, for example, involves identifying an animal in need of an enhanced immune response to an antigen and providing the animal the antigen and an amount of Ribavirin that is effective to enhance an immune response to the antigen. Preferred methods involve providing the animal in need with Ribavirin and a hepatitis antigen (e.g., HBV antigen, HAV antigen, HCV antigen, a nucleic acid encoding these molecules, or any combination thereof). The section below describes the manufacture of vaccines having Ribavirin and an antigen in greater detail. 
     Vaccines Containing Ribavirin 
     The vaccines comprise Ribavirin and an antigen and may contain other ingredients including, but not limited to, adjuvants, binding agents, excipients such as stabilizers (to promote long term storage), emulsifiers, thickening agents, salts, preservatives, solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents and the like. These vaccine preparations are suitable for treatment of animals either as a preventive measure to avoid a disease or condition or as a therapeutic to treat animals already afflicted with a disease or condition. 
     The vaccine compositions can be manufactured in accordance with conventional methods of galenic pharmacy to produce medicinal agents for administration to animals, e.g., mammals including humans. Ribavirin can be obtained from commercial suppliers (e.g., Sigma and ICN). Ribavirin and/or the antigen can be formulated into the vaccine with and without modification. For example, the Ribavirin and/or antigen can be modified or derivatized to make a more stable molecule and/or a more potent adjuvant. By one approach, the stability of Ribavirin and/or an antigen can be enhanced by coupling the molecules to a support such as a hydrophilic polymer (e.g., polyethylene glycol). 
     Many more Ribavirin derivatives can be generated using conventional techniques in rational drug design and combinatorial chemistry. For example, Molecular Simulations Inc. (MSI), as well as many other suppliers, provide software that allows one of skill to build a combinatorial library of organic molecules. The C2.Analog Builder program, for example, can be integrated with MSI&#39;s suite of Cerius2 molecular diversity software to develop a library of Ribavirin derivatives that can be used with the embodiments described herein. (See e.g., http://msi.com/life/products/cerius2/index.html, herein expressly incorporated by reference in its entirety). 
     By one approach, the chemical structure of Ribavirin is recorded on a computer readable media and is accessed by one or more modeling software application programs. The C2.Analog Builder program in conjunction with C2Diversity program allows the user to generate a very large virtual library based on the diversity of R-groups for each substituent position, for example. Compounds having the same structure as the modeled Ribavirin derivatives created in the virtual library are then made using conventional chemistry or can be obtained from a commercial source. 
     The newly manufactured Ribavirin derivatives are then screened in characterization assays, which determine the extent of adjuvant activity of the molecule and/or the extent of its ability to modulate of an immune response. Some characterization assays may involve virtual drug screening software, such as C2.Ludi. C2.Ludi is a software program that allows a user to explore databases of molecules (e.g., Ribavirin derivatives) for their ability to interact with the active site of a protein of interest (e.g., RAC2 or another GTP binding protein). Based upon predicted interactions discovered with the virtual drug screening software, the Ribavirin derivatives can be prioritized for further characterization in conventional assays that determine adjuvant activity and/or the extent of a molecule to modulate an immune response. 
     Example 1 describes a characterization assay that was used to evaluate the adjuvant activity of Ribavirin. 
     EXAMPLE 1 
     This characterization assay can be used with any Ribavirin derivative or combinations of Ribavirin derivatives to determine the extent of adjuvant activity of the particular vaccine formulation. Accordingly, groups of three to five Balb/c mice (BK Universal, Uppsala, Sweden) were immunized i.p or s.c. (e.g., at the base of the tail) with 10 μg or 100 μg of recombinant hepatitis C virus non-structural 3 (NS3) protein. The rNS3 was dissolved in phosphate buffered saline (PBS) alone or PBS containing 1 mg Ribavirin (obtained from ICN, Costa Mesa, Calif.). Mice were injected with a total volume of 100 μl per injection. 
     At two and four weeks following i.p. immunization, all mice were bled by retro-orbital sampling. Serum samples were collected and analyzed for the presence of antibodies to rNS3. To determine the antibody titer, an enzyme immunoassay (EIA) was performed. (See e.g., Hultgren et al.,  J Gen Virol . 79:2381-91 (1998) and Hultgren et al.,  Clin. Diagn. Lab. Immunol . 4:630-632 (1997), both of which are herein expressly incorporated by reference in their entireties). The antibody levels were recorded as the highest serum dilution giving an optical density at 405 nm more than twice that of non-immunized mice. 
     Mice that received 10 μg or 100 μg rNS3 mixed with 1 mg Ribavirin in PBS displayed consistently higher levels of NS3 antibodies. The antibody titer that was detected by EIA at two weeks post-immunization is shown in FIG.  1 . The vaccine formulations having 1 mg of Ribavirin and either 10 μg or 100 μg of rNS3 induced a significantly greater antibody titer than the vaccine formulations composed of only rNS3. This data provides evidence that Ribavirin has an adjuvant effect on the humoral immune response of an animal and thus, enhances the immune response to the antigen. 
     The example below describes experiments that were performed to determine the amount of Ribavirin that was needed to elicit an adjuvant effect. 
     EXAMPLE 2 
     To determine the dose of Ribavirin that is required to provide an adjuvant effect, the following experiments were performed. Groups of mice (three per group) were immunized with a 20 μg rNS3 alone or a mixture of 20 μg rNS3 and 0.1 mg, 1 mg, or 10 mg Ribavirin. The levels of antibody to the antigen were then determined by EIA. The mean endpoint titers at weeks 1 and 3 were plotted and are shown in FIG.  2 . It was discovered that the adjuvant effect provided by Ribavirin had different kinetics depending on the dose of Ribavirin provided. For example, low doses (&lt;1 mg) of Ribavirin were found to enhance antibody levels at week one but not at week three, whereas, higher doses (1-10 mg) were found to enhance antibody levels at week three. These data further verify that Ribavirin can be administered as an adjuvant and establish that that the dose of Ribavirin can modulate the kinetics of the adjuvant effect. 
     The example below describes another characterization assay that was performed to evaluate the ability of Ribavirin to modulate a cellular immune response. 
     EXAMPLE 3 
     This characterization assay can be used with any Ribavirin derivative or combinations of Ribavirin derivatives to determine the extent that a particular vaccine formulation modulates a cellular immune response. To determine CD4 +  T cell responses to Ribavirin-containing vaccine, groups of mice were immunized s.c. with either 100 μg rNS3 in PBS or 100 μg rNS3 and 1 mg Ribavirin in PBS. The mice were sacrificed ten days post-immunization and their lymph nodes were harvested and drained. In vitro recall assays were then performed. (See e.g., Hultgren et al.,  J Gen Virol . 79:2381-91 (1998) and Hultgren et al.,  Clin. Diagn. Lab. Immunol . 4:630-632 (1997), both of which are herein expressly incorporated by reference in their entireties). The amount of CD4 +  T cell proliferation was determined at 96 h of culture by the incorporation of [ 3 H] thymidine. 
     As shown in FIG. 2, mice that were immunized with 100 μg rNS3 mixed with 1 mg Ribavirin had a much greater T cell proliferative response than mice that were immunized with 100 μg rNS3 in PBS. This data provides evidence that Ribavirin can enhance a cellular immune response (e.g., by promoting the effective priming of T cells). 
     The example below describes the use of Ribavirin in conjunction with a commercial vaccine preparation. 
     EXAMPLE 4 
     The adjuvant effect of Ribavirin was also tested when mixed with two doses of a commercially available vaccine containing HBsAg and alum. (Engerix, SKB). Approximately 0.2 μg or 2 μg of Engerix vaccine was mixed with either PBS or 1 mg Ribavirin in PBS and the mixtures were injected intra peritoneally into groups of mice (three per group). A booster containing the same mixture was given on week four and all mice were bled on week six. The serum samples were diluted from 1:60 to 1:37500 and the dilutions were tested by EIA, as described above, except that purified human HBsAg (kindly provided by Professor DL Peterson, Commonwealth University, Va.) was used as the solid phase antigen. As shown in TABLE 1, vaccine formulations having Ribavirin enhanced the response to 2 μg of an existing vaccine despite the fact that the vaccine already contained alum. That is, by adding Ribavirin to a suboptimal vaccine dose (i.e., one that does not induce detectable antibodies alone) antibodies became detectable, providing evidence that the addition of Ribavirin allows for the use of lower antigen amounts in a vaccine formulation without compromising the immune response. 
     
       
         
               
               
             
               
               
               
             
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 1 
               
             
             
               
                   
                   
               
               
                   
                 End point antibody titer to HBsAg in EIA 
               
             
          
           
               
                   
                 0.02 μg Engerix 
                 0.2 μg Engerix 
               
             
          
           
               
                   
                 No Ribavirin 
                 1 mg Ribavirin 
                 No Ribavirin 
                 1 mg Ribavirin 
               
             
          
           
               
                 Week 
                 #1 
                 #2 
                 #3 
                 #1 
                 #2 
                 #3 
                 #1 
                 #2 
                 #3 
                 #1 
                 #2 
                 #3 
               
               
                   
               
               
                 6 
                 &lt;60 
                 &lt;60 
                 &lt;60 
                 &lt;60 
                 &lt;60 
                 &lt;60 
                 &lt;60 
                 &lt;60 
                 &lt;60 
                 300 
                 60 
                 &lt;60 
               
               
                   
               
             
          
         
       
     
     Any antigen that can be used to generate an immune response in an animal can be combined with Ribavirin so as to prepare the vaccines described herein. That is, antigens that can be incorporated into such a vaccine comprise bacterial antigens, fungal antigens, plant antigens, mold antigens, viral antigens, cancer cell antigens, toxin antigens, chemical antigens, and self-antigens. Although many of these antigens are molecules that induce a significant immune response without an adjuvant, Ribavirin can be administered in conjunction with or combined with “strong” or “weak” antigens to enhance the immune response. In addition, the use of Ribavirin as an adjuvant may allow for the use of lower amounts of vaccine antigens while retaining immunogenicity. 
     Preferred embodiments comprise Ribavirin and a viral antigen. Preferred viral antigens are hepatitis viral antigens. Vaccines can comprise, for example, Ribavirin and an HBV antigen, HAV antigen, HCV antigen or any combination of these antigens. Preferred viral antigens include hepatitis B surface antigen (HBsAg), hepatitis core antigen (HBcAg), and hepatitis E antigen (HBeAg). 
     For example, HCV vaccine embodiments comprise Ribavirin and a HCV peptide of at least 3 consecutive amino acids of SEQ. ID. No.: 1. That is, a vaccine embodiment can have Ribavirin and a HCV peptide with a length of at least 3-10 consecutive amino acids, 10-50 consecutive amino acids, 50-100 consecutive amino acids, 100-200 consecutive amino acids, 200-400 consecutive amino acids, 400-800 consecutive amino acids, 800-1200 consecutive amino acids, 1200-1600 consecutive amino acids, 1600-2000 consecutive amino acids, 2000-2500 consecutive amino acids, and 2500-3011 consecutive amino acids of SEQ ID. No. 1. Preferred HCV vaccines comprise Ribavirin and a peptide of at least 3 consecutive amino acids of HCV core protein (SEQ. ID. No. 2), HCV E1 protein (SEQ. ID. No. 3), HCV E2 protein (SEQ. ID. No. 4), HCV NS2 (SEQ. ID. No. 5), HCV NS3 (SEQ. ID. No. 6), HCV NS4A (SEQ. ID. No. 7), HCV NS4B (SEQ. ID. No. 8), or HCV NS5A/B (SEQ. ID. No. 9). That is, preferred HCV vaccines can comprise Ribavirin and a peptide with a length of at least 3-10 consecutive amino acids, 10-50 consecutive amino acids, 50-100 consecutive amino acids, 100-200 consecutive amino acids, 200-400 consecutive amino acids, 400-800 consecutive amino acids, and 800-1040 consecutive amino acids of any one of (SEQ. ID. Nos. 2-9). 
     Similarly, preferred HBV vaccine embodiments comprise Ribavirin and a HBV peptide of at least 3 consecutive amino acids of HBsAg (SEQ. ID. No.: 10) or HBcAg and HBeAg (SEQ. ID. No. 11). That is, a vaccine embodiment can have Ribavirin and a HBV peptide with a length of at least 3-10 consecutive amino acids, 10-50 consecutive amino acids, 50-100 consecutive amino acids, 100-150 consecutive amino acids, 150-200 consecutive amino acids, and 200-226 consecutive amino acids of either SEQ. ID. No. 10 or SEQ. ID. No. 11. Further, preferred HAV embodiments comprise Ribavirin and a HAV peptide with a length of at least 3-10 consecutive amino acids, 10-50 consecutive amino acids, 50-100 consecutive amino acids, 100-200 consecutive amino acids, 200-400 consecutive amino acids, 400-800 consecutive amino acids, 800-1200 consecutive amino acids, 1200-1600 consecutive amino acids, 1600-2000 consecutive amino acids, and 2000-2227 consecutive amino acids of SEQ ID. No. 12. 
     In addition to peptide antigens, nucleic acid-based antigens can be used in the vaccine compositions described herein. Various nucleic acid-based vaccines are known and it is contemplated that these compositions and approaches to immunotherapy can be augmented by introducing Ribavirin (See e.g., U.S. Pat. No. 5,589,466, herein expressly incorporated by reference in its entirety). 
     By one approach, for example, a gene encoding a polypeptide antigen of interest is cloned into an expression vector capable of expressing the polypeptide when introduced into a subject. The expression construct is introduced into the subject in a mixture of Ribavirin or in conjunction with Ribavirin (e.g., Ribavirin is administered shortly after the expression construct at the same site). Alternatively, RNA encoding a polypeptide antigen of interest is provided to the subject in a mixture with Ribavirin or in conjunction with Ribavirin. Where the polynucleotide is to be DNA, promoters suitable for use in various vertebrate systems are well known. For example, for use in murine systems, suitable strong promoters include RSV LTR, MPSV LTR, SV40 IEP, and metallothionein promoter. In humans, on the other hand, promoters such as CMV IEP can be used. All forms of DNA, whether replicating or non-replicating, which do not become integrated into the genome, and which are expressible, can be used. 
     Preferred nucleic acid-based antigens include a nucleotide sequence of at least 9 consecutive nucleotides of HCV (SEQ. ID. No. 13), HBV (SEQ. ID. No.: 14), or HAV (SEQ. ID. No. 15). That is, a nucleic acid based antigen can comprise at least 9-25 consecutive nucleotides, 25-50 consecutive nucleotides, 50-100 consecutive nucleotides, 100-200 consecutive nucleotides, 200-500 consecutive nucleotides, 500-1000 consecutive nucleotides, 1000-2000 consecutive nucleotides, 2000-4000 consecutive nucleotides, 4000-8000 consecutive nucleotides, and 8000-9416 consecutive nucleotides of any one of SEQ. ID. Nos.: 13-15 or an RNA that corresponds to these sequences. 
     The example below describes one approach for using a nucleic acid-based antigen in conjunction with Ribavirin. 
     EXAMPLE 5 
     The following describes an approach to immunize an animal with a vaccine comprising a nucleic acid-based antigen and Ribavirin. Five to six week old female and male Balb/C mice are anesthetized by intraperitoneal injection with 0.3 ml of 2.5% Avertin. A 1.5 cm incision is made on the anterior thigh, and the quadriceps muscle is directly visualized. One group of mice are injected with approximately 20 μg of an expression construct having the gp-120 gene, driven by a cytomegalovirus (CMV) promotor and second group of mice are injected with approximately 5 μg of capped in vitro transcribed RNA (e.g., SP6, T7, or T3 (Ambion)) encoding gp-120. These two groups are controls. A third group of mice is injected with approximately 20 μg of the expression vector having the gp-120 gene and the CMV promoter mixed with 1 mg of Ribavirin and a fourth group of mice is injected with approximately 5 μg of capped in vitro transcribed RNA mixed with 1 mg Rbavirin. The vaccines are injected in 0.1 ml of solution (PBS) in a 1 cc syringe through a 27 gauge needle over one minute, approximately 0.5 cm from the distal insertion site of the muscle into the knee and about 0.2 cm deep. A suture is placed over the injection site for future localization, and the skin is then closed with stainless steel clips. 
     Blood samples are obtained prior to the injection (Day 0) and up to more than 40 days post injection. The serum from each sample is serially diluted and assayed in a standard ELISA technique assay for the detection of antibody, using recombinant gp-120 protein made in yeast as the antigen. Both IgG and IgM antibodies specific for gp-120 will be detected in all samples, however, groups three and four, which contained the Ribavirin, will exhibit a greater immune response to the gp-120 as measured by the amount and/or titer of antibody detected in the sera. 
     Many other ingredients can be present in the vaccine. For example, the Ribavirin and antigen can be employed in admixture with conventional excipients (e.g., pharmaceutically acceptable organic or inorganic carrier substances suitable for parenteral, enteral (e.g., oral) or topical application that do not deleteriously react with the Ribavirin and/or antigen). Suitable pharmaceutically acceptable carriers include, but are not limited to, water, salt solutions, alcohols, gum arabic, vegetable oils, benzyl alcohols, polyetylene glycols, gelatine, carbohydrates such as lactose, amylose or starch, magnesium stearate, talc, silicic acid, viscous paraffin, perfume oil, fatty acid monoglycerides and diglycerides, pentaerythritol fatty acid esters, hydroxy methylcellulose, polyvinyl pyrrolidone, etc. Many more suitable carriers are described in  Remmington&#39;s  Pharmaceutical Sciences, 15th Edition, Easton:Mack Publishing Company, pages 1405-1412 and 1461-1487(1975) and The National  Formulary  XIV, 14th Edition, Washington, American Pharmaceutical Association (1975), herein expressly incorporated by reference in their entireties. Vaccines can be sterilized and if desired mixed with auxiliary agents, e.g., lubricants, preservatives, stabilizers, wetting agents, emulsifiers, salts for influencing osmotic pressure, buffers, coloring, flavoring and/or aromatic substances and the like that do not deleteriously react with Ribavirin or the antigen. 
     The effective dose and method of administration of a particular vaccine formulation can vary based on the individual patient and the type and stage of the disease, as well as other factors known to those of skill in the art. Therapeutic efficacy and toxicity of the vaccines can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., ED50 (the dose therapeutically effective in 50% of the population). The data obtained from cell culture assays and animal studies can be used to formulate a range of dosage for human use. The dosage of the vaccines lies preferably within a range of circulating concentrations that include the ED50 with no toxicity. The dosage varies within this range depending upon the type of Ribavirin derivative and antigen, the dosage form employed, the sensitivity of the patient, and the route of administration. 
     Since Ribavirin has been on the market for several years, many dosage forms and routes of administration are known. All known dosage forms and routes of administration can be provided within the context of the embodiments described herein. Preferably, an amount of Ribavirin that is effective to enhance an immune response to an antigen in an animal can be considered to be an amount that is sufficient to achieve a blood serum level of antigen approximately 0.25-12.5 μg/ml in the animal, preferably, about 2.5 μg/ml. In some embodiments, the amount of Ribavirin is determined according to the body weight of the animal to be given the vaccine. Accordingly, the amount of Ribavirin in a vaccine formulation can be from about 0.1-6.0 mg/kg body weight. That is, some embodiments have an amount of Ribavirin that corresponds to approximately 0.1-1.0 mg/kg, 1.1-2.0 mg/kg, 2.1-3.0 mg/kg, 3.1-4.0 mg/kg, 4.1-5.0 mg/kg, 5.1, and 6.0 mg/kg body weight of an animal. More conventionally, the vaccines contain approximately 0.25 mg-2000 mg of Ribavirin. That is, some embodiments have approximately 250 μg, 500 μg, 1 mg, 25 mg, 50 mg, 100 mg, 150 mg, 200 mg, 250 mg, 300 mg, 350 mg, 400 mg, 450 mg, 500 mg, 550 mg, 600 mg, 650 mg, 700 mg, 750 mg, 800 mg, 850 mg, 900 mg, 1 g, 1.1 g, 1.2 g, 1.3 g, 1.4 g, 1.5 g, 1.6 g, 1.7 g, 1.8 g, 1.9 g, and 2 g of Ribavirin. 
     Vaccines comprising various antigens and amounts of these antigens have been provided to animals for many years. Thus, conventional vaccine preparations can be modified by adding an amount of Ribavirin that is sufficient to enhance an immune response to the antigen. That is, existing conventional vaccine formulations can be modified by simply adding Ribavirin to the preparation or by administering the conventional vaccine in conjunction with Ribavirin (e.g., shortly before or after providing the antigen). As one of skill in the art will appreciate, the amount of antigens in a vaccine can vary depending on the type of antigen and its immunogenicity. The amount of antigens in the vaccines can vary accordingly. Nevertheless, as a general guide, the vaccines can have approximately 0.25 mg-2000 mg of an antigen (e.g., a hepatitis viral antigen). 
     In some approaches described herein, the exact amount of Ribavirin and/or antigen is chosen by the individual physician in view of the patient to be treated. Further, the amounts of Ribavirin can be added in combination to or separately from the same or equivalent amount of antigen and these amounts can be adjusted during a particular vaccination protocol so as to provide sufficient levels in light of patient-specific or antigen-specific considerations. In this vein, patient-specific and antigen-specific factors that can be taken into account include, but are not limited to, the severity of the disease state of the patient, age, and weight of the patient, diet, time and frequency of administration, drug combination(s), reaction sensitivities, and tolerance/response to therapy. 
     Routes of administration of the vaccines described herein include, but are not limited to, transdermal, parenteral, gastrointestinal, transbronchial, and transalveolar. Transdermal administration can be accomplished by application of a cream, rinse, gel, etc. capable of allowing Ribavirin and antigen to penetrate the skin. Parenteral routes of administration include, but are not limited to, electrical or direct injection such as direct injection into a central venous line, intravenous, intramuscular, intraperitoneal, intradermal, or subcutaneous injection. Gastrointestinal routes of administration include, but are not limited to, ingestion and rectal. Transbronchial and transalveolar routes of administration include, but are not limited to, inhalation, either via the mouth or intranasally. 
     Compositions having Ribavirin and an antigen that are suitable for transdermal administration include, but are not limited to, pharmaceutically acceptable suspensions, oils, creams, and ointments applied directly to the skin or incorporated into a protective carrier such as a transdermal device (“transdermal patch”). Examples of suitable creams, ointments, etc. can be found, for instance, in the Physician&#39;s Desk Reference. Examples of suitable transdermal devices are described, for instance, in U.S. Pat. No. 4,818,540 issued Apr. 4, 1989 to Chinen, et al., herein expressly incorporated by reference in its entirety. 
     Compositions having Ribavirin and an antigen that are suitable for parenteral administration include, but are not limited to, pharmaceutically acceptable sterile isotonic solutions. Such solutions include, but are not limited to, saline, phosphate buffered saline and oil preparations for injection into a central venous line, intravenous, intramuscular, intraperitoneal, intradermal, or subcutaneous injection. 
     Compositions having Ribavirin and an antigen that are suitable for transbronchial and transalveolar administration include, but not limited to, various types of aerosols for inhalation. Devices suitable for transbronchial and transalveolar administration of these are also embodiments. Such devices include, but are not limited to, atomizers and vaporizers. Many forms of currently available atomizers and vaporizers can be readily adapted to deliver vaccines having Ribavirin and an antigen. 
     Compositions having Ribavirin and an antigen that are suitable for gastrointestinal administration include, but not limited to, pharmaceutically acceptable powders, pills or liquids for ingestion and suppositories for rectal administration. 
     Once the vaccine comprising Ribavirin and an antigen has been obtained, it can be administered to a subject in need to treat or prevent diseases. The next section describes methods that employ the vaccines described above. 
     Methods of use of Vaccines that Contain Ribavirin 
     The vaccines containing Ribavirin and an antigen can be used to treat and prevent a vast spectrum of diseases and can enhance the immune response of an animal to an antigen. As one of skill in the art will appreciate conventional vaccines have been administered to subjects in need of treatment or prevention of bacterial diseases, viral diseases, fungal diseases, and cancer. Because the vaccines described herein include conventional vaccines, which have been modified by the addition of Ribavirin, the methods described herein include the treatment and prevention of a disease using a vaccine that comprises an antigen and Ribavirin. 
     Preferred embodiments concern methods of treating or preventing hepatitis infection. In these embodiments, an animal in need is provided a hepatitis antigen (e.g., a peptide antigen or nucleic acid-based antigen) and an amount of Ribavirin sufficient to exhibit an adjuvant activity in said animal. Accordingly, an animal can be identified as one in need by using currently available diagnostic testing or clinical evaluation. The range of hepatitis viral antigens that can be used with these embodiments is diverse. Preferred hepatitis viral antigens include an HBV antigen, an HAV antigen, an HCV antigen, nucleic acids encoding these antigens, or any combination thereof. Highly preferred embodiments include an HBV antigen selected from the group consisting of hepatitis B surface antigen (HBsAg), hepatitis core antigen (HBcAg), and hepatitis E antigen (HBeAg), in particular, the peptide and nucleic acid-based antigens describes supra. The Ribavirin and antigen can be provided separately or in combination, and other adjuvants (e.g., oil, alum, or other agents that enhance an immune response) can also be provided to the animal in need. Thus, preferred embodiments include methods of treating or preventing hepatitis in an animal (e.g., HBV) by identifying an infected animal or an animal at risk of infection and providing said animal a hepatitis antigen (e.g., HBsAg, HBcAg, and HBeAg) and an amount of Ribavirin sufficient to exhibit adjuvant activity. 
     Other embodiments include methods of enhancing an immune response to an antigen by providing an animal in need with an amount of Ribavirin that is effective to enhance said immune response. In these embodiments, an animal in need of an enhanced immune response to an antigen is identified by using currently available diagnostic testing or clinical evaluation. Oftentimes these individuals will be suffering from a disease (e.g., bacterial, fungal, mold, viral, or cancer) or are at risk from contracting the disease. However, an animal in need of an enhanced immune response can be an animal that has been poisoned (e.g., bit by a poisonous insect or animal) or that has been exposed to a toxin or other toxic compound. Once identified, these animals are provided an appropriate antigen and an amount of Ribavirin effective to enhance an immune response in the animal. 
     As above, the hepatitis viral antigens that can be used with these embodiments include, but are not limited to, an HBV antigen, an HAV antigen, an HCV antigen, a nucleic acid encoding these molecules, or any combination thereof. Highly preferred embodiments include an HBV antigen selected from the group consisting of hepatitis B surface antigen (HBsAg), hepatitis core antigen (HBcAg), and hepatitis E antigen (HBeAg), in particular, the peptide and nucleic acid-based antigens described supra. The Ribavirin and antigen can be provided separately or in combination, and other adjuvants (e.g., oil, alum, or other agents that enhance an immune response) can also be provided to the animal in need. Thus, preferred embodiments include methods of enhancing an immune response to a hepatitis antigen (e.g., HBV) by identifying an animal in need and providing the animal a hepatitis antigen (e.g., HBsAg, HBcAg, and HBeAg) and an amount of Ribavirin that is effective to enhance an immune response in the animal. 
     Although the invention has been described with reference to embodiments and examples, it should be understood that various modifications can be made without departing from the spirit of the invention. Accordingly, the invention is limited only by the following claims. All references cited herein are hereby expressly incorporated by reference. 
     
       
         
               
             
           
               
                   
               
             
             
               
                                    
               
               
                 #             SEQUENCE LISTING  
               
               
                   
               
               
                   
               
               
                 &lt;160&gt; NUMBER OF SEQ ID NOS: 15  
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 1  
               
               
                 &lt;211&gt; LENGTH: 3011  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus sequenc  
               
               
                 #e  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 1  
               
               
                   
               
               
                 Met Ser Thr Asn Pro Lys Pro Gln Arg Lys Th  
               
               
                 #r Lys Arg Asn Thr Asn  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Arg Arg Pro Gln Asp Val Lys Phe Pro Gly Gl  
               
               
                 #y Gly Gln Ile Val Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Gly Val Tyr Leu Leu Pro Arg Arg Gly Pro Ar  
               
               
                 #g Leu Gly Val Arg Ala  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Thr Arg Lys Thr Ser Glu Arg Ser Gln Pro Ar  
               
               
                 #g Gly Arg Arg Gln Pro  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Ile Pro Lys Ala Arg Arg Pro Glu Gly Arg Th  
               
               
                 #r Trp Ala Gln Pro Gly  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Tyr Pro Trp Pro Leu Tyr Gly Asn Glu Gly Cy  
               
               
                 #s Gly Trp Ala Gly Trp  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Leu Leu Ser Pro Arg Gly Ser Arg Pro Ser Tr  
               
               
                 #p Gly Pro Thr Asp Pro  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Arg Arg Arg Ser Arg Asn Leu Gly Lys Val Il  
               
               
                 #e Asp Thr Leu Thr Cys  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Gly Phe Ala Asp Leu Met Gly Tyr Ile Pro Le  
               
               
                 #u Val Gly Ala Pro Leu  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Gly Gly Ala Ala Arg Ala Leu Ala His Gly Va  
               
               
                 #l Arg Val Leu Glu Asp  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Gly Val Asn Tyr Ala Thr Gly Asn Leu Pro Gl  
               
               
                 #y Cys Ser Phe Ser Ile  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Phe Leu Leu Ala Leu Leu Ser Cys Leu Thr Va  
               
               
                 #l Pro Ala Ser Ala Tyr  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Gln Val Arg Asn Ser Ser Gly Leu Tyr His Va  
               
               
                 #l Thr Asn Asp Cys Pro  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Asn Ser Ser Val Val Tyr Glu Ala Ala Asp Al  
               
               
                 #a Ile Leu His Thr Pro  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Gly Cys Val Pro Cys Val Arg Glu Gly Asn Al  
               
               
                 #a Ser Arg Cys Trp Val  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gl  
               
               
                 #y Lys Leu Pro Thr Thr  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Gln Leu Arg Arg His Ile Asp Leu Leu Val Gl  
               
               
                 #y Ser Ala Thr Leu Cys  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Se  
               
               
                 #r Val Phe Leu Val Gly  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Gln Leu Phe Thr Phe Ser Pro Arg His His Tr  
               
               
                 #p Thr Thr Gln Asp Cys  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gl  
               
               
                 #y His Arg Met Ala Trp  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Asn Met Met Met Asn Trp Ser Pro Thr Ala Al  
               
               
                 #a Leu Val Val Ala Gln  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Me  
               
               
                 #t Ile Ala Gly Ala His  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Trp Gly Val Leu Ala Gly Ile Lys Tyr Phe Se  
               
               
                 #r Met Val Gly Asn Trp  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Ala Lys Val Leu Val Val Leu Leu Leu Phe Al  
               
               
                 #a Gly Val Asp Ala Glu  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Thr His Val Thr Gly Gly Asn Ala Gly Arg Th  
               
               
                 #r Thr Ala Gly Leu Val  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Il  
               
               
                 #e Gln Leu Ile Asn Thr  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Asn Gly Ser Trp His Ile Asn Ser Thr Ala Le  
               
               
                 #u Asn Cys Asn Glu Ser  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Ty  
               
               
                 #r Gln His Lys Phe Asn  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cy  
               
               
                 #s Arg Arg Leu Thr Asp  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Al  
               
               
                 #a Asn Gly Ser Gly Leu  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pr  
               
               
                 #o Arg Pro Cys Gly Ile  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Val Pro Ala Lys Ser Val Cys Gly Pro Val Ty  
               
               
                 #r Cys Phe Thr Pro Ser  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Pro Val Val Val Gly Thr Thr Asp Arg Ser Gl  
               
               
                 #y Ala Pro Thr Tyr Ser  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Trp Gly Ala Asn Asp Thr Asp Val Phe Val Le  
               
               
                 #u Asn Asn Thr Arg Pro  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Me  
               
               
                 #t Asn Ser Thr Gly Phe  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Thr Lys Val Cys Gly Ala Pro Pro Cys Val Il  
               
               
                 #e Gly Gly Val Gly Asn  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Ar  
               
               
                 #g Lys Tyr Pro Glu Ala  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Thr Tyr Ser Arg Cys Gly Ser Gly Pro Arg Il  
               
               
                 #e Thr Pro Arg Cys Met  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pr  
               
               
                 #o Cys Thr Ile Asn Tyr  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gl  
               
               
                 #y Val Glu His Arg Leu  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Ar  
               
               
                 #g Cys Asp Leu Glu Asp  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Le  
               
               
                 #u Ser Thr Thr Gln Trp  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pr  
               
               
                 #o Ala Leu Ser Thr Gly  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Leu Ile His Leu His Gln Asn Ile Val Asp Va  
               
               
                 #l Gln Tyr Leu Tyr Gly  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Val Gly Ser Ser Ile Ala Ser Trp Ala Ile Ly  
               
               
                 #s Trp Glu Tyr Val Val  
               
               
                 705                 7  
               
               
                 #10                 7  
               
               
                 #15                 7  
               
               
                 #20  
               
               
                   
               
               
                 Leu Leu Phe Leu Leu Leu Ala Asp Ala Arg Va  
               
               
                 #l Cys Ser Cys Leu Trp  
               
               
                                 725   
               
               
                 #               730   
               
               
                 #               735  
               
               
                   
               
               
                 Met Met Leu Leu Ile Ser Gln Ala Glu Ala Al  
               
               
                 #a Leu Glu Asn Leu Val  
               
               
                             740       
               
               
                 #           745       
               
               
                 #           750  
               
               
                   
               
               
                 Ile Leu Asn Ala Ala Ser Leu Ala Gly Thr Hi  
               
               
                 #s Gly Leu Val Ser Phe  
               
               
                         755           
               
               
                 #       760           
               
               
                 #       765  
               
               
                   
               
               
                 Leu Val Phe Phe Cys Phe Ala Trp Tyr Leu Ly  
               
               
                 #s Gly Arg Trp Val Pro  
               
               
                     770               
               
               
                 #   775               
               
               
                 #   780  
               
               
                   
               
               
                 Gly Ala Val Tyr Ala Leu Tyr Gly Met Trp Pr  
               
               
                 #o Leu Leu Leu Leu Leu  
               
               
                 785                 7  
               
               
                 #90                 7  
               
               
                 #95                 8  
               
               
                 #00  
               
               
                   
               
               
                 Leu Ala Leu Pro Gln Arg Ala Tyr Ala Leu As  
               
               
                 #p Thr Glu Val Ala Ala  
               
               
                                 805   
               
               
                 #               810   
               
               
                 #               815  
               
               
                   
               
               
                 Ser Cys Gly Gly Val Val Leu Val Gly Leu Me  
               
               
                 #t Ala Leu Thr Leu Ser  
               
               
                             820       
               
               
                 #           825       
               
               
                 #           830  
               
               
                   
               
               
                 Pro Tyr Tyr Lys Arg Tyr Ile Ser Trp Cys Me  
               
               
                 #t Trp Trp Leu Gln Tyr  
               
               
                         835           
               
               
                 #       840           
               
               
                 #       845  
               
               
                   
               
               
                 Phe Leu Thr Arg Val Glu Ala Gln Leu His Va  
               
               
                 #l Trp Val Pro Pro Leu  
               
               
                     850               
               
               
                 #   855               
               
               
                 #   860  
               
               
                   
               
               
                 Asn Val Arg Gly Gly Arg Asp Ala Val Ile Le  
               
               
                 #u Leu Thr Cys Val Val  
               
               
                 865                 8  
               
               
                 #70                 8  
               
               
                 #75                 8  
               
               
                 #80  
               
               
                   
               
               
                 His Pro Ala Leu Val Phe Asp Ile Thr Lys Le  
               
               
                 #u Leu Leu Ala Ile Phe  
               
               
                                 885   
               
               
                 #               890   
               
               
                 #               895  
               
               
                   
               
               
                 Gly Pro Leu Trp Ile Leu Gln Ala Ser Leu Le  
               
               
                 #u Lys Val Pro Tyr Phe  
               
               
                             900       
               
               
                 #           905       
               
               
                 #           910  
               
               
                   
               
               
                 Val Arg Val Gln Gly Leu Leu Arg Ile Cys Al  
               
               
                 #a Leu Ala Arg Lys Ile  
               
               
                         915           
               
               
                 #       920           
               
               
                 #       925  
               
               
                   
               
               
                 Ala Gly Gly His Tyr Val Gln Met Ala Ile Il  
               
               
                 #e Lys Leu Gly Ala Leu  
               
               
                     930               
               
               
                 #   935               
               
               
                 #   940  
               
               
                   
               
               
                 Thr Gly Thr Cys Val Tyr Asn His Leu Ala Pr  
               
               
                 #o Leu Arg Asp Trp Ala  
               
               
                 945                 9  
               
               
                 #50                 9  
               
               
                 #55                 9  
               
               
                 #60  
               
               
                   
               
               
                 His Asn Gly Leu Arg Asp Leu Ala Val Ala Va  
               
               
                 #l Glu Pro Val Val Phe  
               
               
                                 965   
               
               
                 #               970   
               
               
                 #               975  
               
               
                   
               
               
                 Ser Arg Met Glu Thr Lys Leu Ile Thr Trp Gl  
               
               
                 #y Ala Asp Thr Ala Ala  
               
               
                             980       
               
               
                 #           985       
               
               
                 #           990  
               
               
                   
               
               
                 Cys Gly Asp Ile Ile Asn Gly Leu Pro Val Se  
               
               
                 #r Ala Arg Arg Gly Gln  
               
               
                         995           
               
               
                 #       1000           
               
               
                 #      1005  
               
               
                   
               
               
                 Glu Ile Leu Leu Gly Pro Ala Asp Gly Met Va  
               
               
                 #l Ser Lys Gly Trp Arg  
               
               
                     1010              
               
               
                 #   1015               
               
               
                 #  1020  
               
               
                   
               
               
                 Leu Leu Ala Pro Ile Thr Ala Tyr Ala Gln Gl  
               
               
                 #n Thr Arg Gly Leu Leu  
               
               
                 1025                1030  
               
               
                 #                1035  
               
               
                 #               1040  
               
               
                   
               
               
                 Gly Cys Ile Ile Thr Ser Leu Thr Gly Arg As  
               
               
                 #p Lys Asn Gln Val Glu  
               
               
                                 1045  
               
               
                 #               1050   
               
               
                 #              1055  
               
               
                   
               
               
                 Gly Glu Val Gln Ile Val Ser Thr Ala Thr Gl  
               
               
                 #n Thr Phe Leu Ala Thr  
               
               
                             1060      
               
               
                 #           1065       
               
               
                 #          1070  
               
               
                   
               
               
                 Cys Ile Asn Gly Val Cys Trp Thr Val Tyr Hi  
               
               
                 #s Gly Ala Gly Thr Arg  
               
               
                         1075          
               
               
                 #       1080           
               
               
                 #      1085  
               
               
                   
               
               
                 Thr Ile Ala Ser Pro Lys Gly Pro Val Ile Gl  
               
               
                 #n Thr Tyr Thr Asn Val  
               
               
                     1090              
               
               
                 #   1095               
               
               
                 #  1100  
               
               
                   
               
               
                 Asp Gln Asp Leu Val Gly Trp Pro Ala Pro Gl  
               
               
                 #n Gly Ser Arg Ser Leu  
               
               
                 1105                1110  
               
               
                 #                1115  
               
               
                 #               1120  
               
               
                   
               
               
                 Thr Pro Cys Thr Cys Gly Ser Ser Asp Leu Ty  
               
               
                 #r Leu Val Thr Arg His  
               
               
                                 1125  
               
               
                 #               1130   
               
               
                 #              1135  
               
               
                   
               
               
                 Ala Asp Val Ile Pro Val Arg Arg Arg Gly As  
               
               
                 #p Ser Arg Gly Ser Leu  
               
               
                             1140      
               
               
                 #           1145       
               
               
                 #          1150  
               
               
                   
               
               
                 Leu Ser Pro Arg Pro Ile Ser Tyr Leu Lys Gl  
               
               
                 #y Ser Ser Gly Gly Pro  
               
               
                         1155          
               
               
                 #       1160           
               
               
                 #      1165  
               
               
                   
               
               
                 Leu Leu Cys Pro Thr Gly His Ala Val Gly Le  
               
               
                 #u Phe Arg Ala Ala Val  
               
               
                     1170              
               
               
                 #   1175               
               
               
                 #  1180  
               
               
                   
               
               
                 Cys Thr Arg Gly Val Ala Lys Ala Val Asp Ph  
               
               
                 #e Ile Pro Val Glu Asn  
               
               
                 1185                1190  
               
               
                 #                1195  
               
               
                 #               1200  
               
               
                   
               
               
                 Leu Glu Thr Thr Met Arg Ser Pro Val Phe Th  
               
               
                 #r Asp Asn Ser Ser Pro  
               
               
                                 1205  
               
               
                 #               1210   
               
               
                 #              1215  
               
               
                   
               
               
                 Pro Ala Val Pro Gln Ser Phe Gln Val Ala Hi  
               
               
                 #s Leu His Ala Pro Thr  
               
               
                             1220      
               
               
                 #           1225       
               
               
                 #          1230  
               
               
                   
               
               
                 Gly Ser Gly Lys Ser Thr Lys Val Pro Ala Al  
               
               
                 #a Tyr Ala Ala Lys Gly  
               
               
                         1235          
               
               
                 #       1240           
               
               
                 #      1245  
               
               
                   
               
               
                 Tyr Lys Val Leu Val Leu Asn Pro Ser Val Al  
               
               
                 #a Ala Thr Leu Gly Phe  
               
               
                     1250              
               
               
                 #   1255               
               
               
                 #  1260  
               
               
                   
               
               
                 Gly Ala Tyr Met Ser Lys Ala His Gly Val As  
               
               
                 #p Pro Asn Ile Arg Thr  
               
               
                 1265                1270  
               
               
                 #                1275  
               
               
                 #               1280  
               
               
                   
               
               
                 Gly Val Arg Thr Ile Thr Thr Gly Ser Pro Il  
               
               
                 #e Thr Tyr Ser Thr Tyr  
               
               
                                 1285  
               
               
                 #               1290   
               
               
                 #              1295  
               
               
                   
               
               
                 Gly Lys Phe Leu Ala Asp Ala Gly Cys Ser Gl  
               
               
                 #y Gly Ala Tyr Asp Ile  
               
               
                             1300      
               
               
                 #           1305       
               
               
                 #          1310  
               
               
                   
               
               
                 Ile Ile Cys Asp Glu Cys His Ser Thr Asp Al  
               
               
                 #a Thr Ser Ile Ser Gly  
               
               
                         1315          
               
               
                 #       1320           
               
               
                 #      1325  
               
               
                   
               
               
                 Ile Gly Thr Val Leu Asp Gln Ala Glu Thr Al  
               
               
                 #a Gly Ala Arg Leu Val  
               
               
                     1330              
               
               
                 #   1335               
               
               
                 #  1340  
               
               
                   
               
               
                 Val Leu Ala Thr Ala Thr Pro Pro Gly Ser Va  
               
               
                 #l Thr Val Ser His Pro  
               
               
                 1345                1350  
               
               
                 #                1355  
               
               
                 #               1360  
               
               
                   
               
               
                 Asn Ile Glu Glu Val Ala Leu Ser Thr Thr Gl  
               
               
                 #y Glu Ile Pro Phe Tyr  
               
               
                                 1365  
               
               
                 #               1370   
               
               
                 #              1375  
               
               
                   
               
               
                 Gly Lys Ala Ile Pro Leu Glu Val Ile Lys Gl  
               
               
                 #y Gly Arg His Leu Ile  
               
               
                             1380      
               
               
                 #           1385       
               
               
                 #          1390  
               
               
                   
               
               
                 Phe Cys His Ser Lys Lys Lys Cys Asp Glu Le  
               
               
                 #u Ala Ala Lys Leu Val  
               
               
                         1395          
               
               
                 #       1400           
               
               
                 #      1405  
               
               
                   
               
               
                 Ala Leu Gly Ile Asn Ala Val Ala Tyr Tyr Ar  
               
               
                 #g Gly Leu Asp Val Ser  
               
               
                     1410              
               
               
                 #   1415               
               
               
                 #  1420  
               
               
                   
               
               
                 Val Ile Pro Thr Ser Gly Asp Val Val Val Va  
               
               
                 #l Ser Thr Asp Ala Leu  
               
               
                 1425                1430  
               
               
                 #                1435  
               
               
                 #               1440  
               
               
                   
               
               
                 Met Thr Gly Phe Thr Gly Asp Phe Asp Ser Va  
               
               
                 #l Ile Asp Cys Asn Thr  
               
               
                                 1445  
               
               
                 #               1450   
               
               
                 #              1455  
               
               
                   
               
               
                 Cys Val Thr Gln Thr Val Asp Phe Ser Leu As  
               
               
                 #p Pro Thr Phe Thr Ile  
               
               
                             1460      
               
               
                 #           1465       
               
               
                 #          1470  
               
               
                   
               
               
                 Glu Thr Thr Thr Leu Pro Gln Asp Ala Val Se  
               
               
                 #r Arg Thr Gln Arg Arg  
               
               
                         1475          
               
               
                 #       1480           
               
               
                 #      1485  
               
               
                   
               
               
                 Gly Arg Thr Gly Arg Gly Lys Pro Gly Ile Ty  
               
               
                 #r Arg Phe Val Ala Pro  
               
               
                     1490              
               
               
                 #   1495               
               
               
                 #  1500  
               
               
                   
               
               
                 Gly Glu Arg Pro Ser Gly Met Phe Asp Ser Se  
               
               
                 #r Val Leu Cys Glu Cys  
               
               
                 1505                1510  
               
               
                 #                1515  
               
               
                 #               1520  
               
               
                   
               
               
                 Tyr Asp Ala Gly Cys Ala Trp Tyr Glu Leu Th  
               
               
                 #r Pro Ala Glu Thr Thr  
               
               
                                 1525  
               
               
                 #               1530   
               
               
                 #              1535  
               
               
                   
               
               
                 Val Arg Leu Arg Ala Tyr Met Asn Thr Pro Gl  
               
               
                 #y Leu Pro Val Cys Gln  
               
               
                             1540      
               
               
                 #           1545       
               
               
                 #          1550  
               
               
                   
               
               
                 Asp His Leu Gly Phe Trp Glu Gly Val Phe Th  
               
               
                 #r Gly Leu Thr His Ile  
               
               
                         1555          
               
               
                 #       1560           
               
               
                 #      1565  
               
               
                   
               
               
                 Asp Ala His Phe Leu Ser Gln Thr Lys Gln Se  
               
               
                 #r Gly Glu Asn Phe Pro  
               
               
                     1570              
               
               
                 #   1575               
               
               
                 #  1580  
               
               
                   
               
               
                 Tyr Leu Val Ala Tyr Gln Ala Thr Val Cys Al  
               
               
                 #a Arg Ala Gln Ala Pro  
               
               
                 1585                1590  
               
               
                 #                1595  
               
               
                 #               1600  
               
               
                   
               
               
                 Pro Pro Ser Trp Asp Gln Met Arg Lys Cys Le  
               
               
                 #u Ile Arg Leu Lys Pro  
               
               
                                 1605  
               
               
                 #               1610   
               
               
                 #              1615  
               
               
                   
               
               
                 Thr Leu His Gly Pro Thr Pro Leu Leu Tyr Ar  
               
               
                 #g Leu Gly Ala Val Gln  
               
               
                             1620      
               
               
                 #           1625       
               
               
                 #          1630  
               
               
                   
               
               
                 Asn Glu Val Thr Leu Thr His Pro Ile Thr Ly  
               
               
                 #s Tyr Ile Met Thr Cys  
               
               
                         1635          
               
               
                 #       1640           
               
               
                 #      1645  
               
               
                   
               
               
                 Met Ser Ala Asp Leu Glu Val Val Thr Ser Th  
               
               
                 #r Trp Val Leu Val Gly  
               
               
                     1650              
               
               
                 #   1655               
               
               
                 #  1660  
               
               
                   
               
               
                 Gly Val Leu Ala Ala Leu Ala Ala Tyr Cys Le  
               
               
                 #u Ser Thr Gly Cys Val  
               
               
                 1665                1670  
               
               
                 #                1675  
               
               
                 #               1680  
               
               
                   
               
               
                 Val Ile Val Gly Arg Ile Val Leu Ser Gly Ly  
               
               
                 #s Pro Ala Ile Ile Pro  
               
               
                                 1685  
               
               
                 #               1690   
               
               
                 #              1695  
               
               
                   
               
               
                 Asp Arg Glu Val Leu Tyr Gln Glu Phe Asp Gl  
               
               
                 #u Met Glu Glu Cys Ser  
               
               
                             1700      
               
               
                 #           1705       
               
               
                 #          1710  
               
               
                   
               
               
                 Gln His Leu Pro Tyr Ile Glu Gln Gly Met Me  
               
               
                 #t Leu Ala Glu Gln Phe  
               
               
                         1715          
               
               
                 #       1720           
               
               
                 #      1725  
               
               
                   
               
               
                 Lys Gln Lys Ala Leu Gly Leu Leu Gln Thr Al  
               
               
                 #a Ser Arg His Ala Glu  
               
               
                     1730              
               
               
                 #   1735               
               
               
                 #  1740  
               
               
                   
               
               
                 Val Ile Thr Pro Ala Val Gln Thr Asn Trp Gl  
               
               
                 #n Lys Leu Glu Val Phe  
               
               
                 1745                1750  
               
               
                 #                1755  
               
               
                 #               1760  
               
               
                   
               
               
                 Trp Ala Lys His Met Trp Asn Phe Ile Ser Gl  
               
               
                 #y Ile Gln Tyr Leu Ala  
               
               
                                 1765  
               
               
                 #               1770   
               
               
                 #              1775  
               
               
                   
               
               
                 Gly Leu Ser Thr Leu Pro Gly Asn Pro Ala Il  
               
               
                 #e Ala Ser Leu Met Ala  
               
               
                             1780      
               
               
                 #           1785       
               
               
                 #          1790  
               
               
                   
               
               
                 Phe Thr Ala Ala Val Thr Ser Pro Leu Thr Th  
               
               
                 #r Gly Gln Thr Leu Leu  
               
               
                         1795          
               
               
                 #       1800           
               
               
                 #      1805  
               
               
                   
               
               
                 Phe Asn Ile Leu Gly Gly Trp Val Ala Ala Gl  
               
               
                 #n Leu Ala Ala Pro Gly  
               
               
                     1810              
               
               
                 #   1815               
               
               
                 #  1820  
               
               
                   
               
               
                 Ala Ala Thr Ala Phe Val Gly Ala Gly Leu Al  
               
               
                 #a Gly Ala Ala Leu Asp  
               
               
                 1825                1830  
               
               
                 #                1835  
               
               
                 #               1840  
               
               
                   
               
               
                 Ser Val Gly Leu Gly Lys Val Leu Val Asp Il  
               
               
                 #e Leu Ala Gly Tyr Gly  
               
               
                                 1845  
               
               
                 #               1850   
               
               
                 #              1855  
               
               
                   
               
               
                 Ala Gly Val Ala Gly Ala Leu Val Ala Phe Ly  
               
               
                 #s Ile Met Ser Gly Glu  
               
               
                             1860      
               
               
                 #           1865       
               
               
                 #          1870  
               
               
                   
               
               
                 Val Pro Ser Thr Glu Asp Leu Val Asn Leu Le  
               
               
                 #u Pro Ala Ile Leu Ser  
               
               
                         1875          
               
               
                 #       1880           
               
               
                 #      1885  
               
               
                   
               
               
                 Pro Gly Ala Leu Ala Val Gly Val Val Phe Al  
               
               
                 #a Ser Ile Leu Arg Arg  
               
               
                     1890              
               
               
                 #   1895               
               
               
                 #  1900  
               
               
                   
               
               
                 Arg Val Gly Pro Gly Glu Gly Ala Val Gln Tr  
               
               
                 #p Met Asn Arg Leu Ile  
               
               
                 1905                1910  
               
               
                 #                1915  
               
               
                 #               1920  
               
               
                   
               
               
                 Ala Phe Ala Ser Arg Gly Asn His Val Ser Pr  
               
               
                 #o Thr His Tyr Val Pro  
               
               
                                 1925  
               
               
                 #               1930   
               
               
                 #              1935  
               
               
                   
               
               
                 Glu Ser Asp Ala Ala Ala Arg Val Thr Ala Il  
               
               
                 #e Leu Ser Ser Leu Thr  
               
               
                             1940      
               
               
                 #           1945       
               
               
                 #          1950  
               
               
                   
               
               
                 Val Thr Gln Leu Leu Arg Arg Leu His Gln Tr  
               
               
                 #p Ile Ser Ser Glu Cys  
               
               
                         1955          
               
               
                 #       1960           
               
               
                 #      1965  
               
               
                   
               
               
                 Thr Thr Pro Cys Ser Gly Ser Trp Leu Arg As  
               
               
                 #p Ile Trp Asp Trp Ile  
               
               
                     1970              
               
               
                 #   1975               
               
               
                 #  1980  
               
               
                   
               
               
                 Cys Glu Val Leu Ser Asp Phe Lys Thr Trp Le  
               
               
                 #u Lys Ala Lys Leu Met  
               
               
                 1985                1990  
               
               
                 #                1995  
               
               
                 #               2000  
               
               
                   
               
               
                 Pro Gln Leu Pro Gly Ile Pro Phe Val Ser Cy  
               
               
                 #s Gln Arg Gly Tyr Arg  
               
               
                                 2005  
               
               
                 #               2010   
               
               
                 #              2015  
               
               
                   
               
               
                 Gly Val Trp Arg Gly Asp Gly Ile Met His Th  
               
               
                 #r Arg Cys His Cys Gly  
               
               
                             2020      
               
               
                 #           2025       
               
               
                 #          2030  
               
               
                   
               
               
                 Ala Glu Ile Thr Gly His Val Lys Asn Gly Th  
               
               
                 #r Met Arg Ile Val Gly  
               
               
                         2035          
               
               
                 #       2040           
               
               
                 #      2045  
               
               
                   
               
               
                 Pro Arg Thr Cys Lys Asn Met Trp Ser Gly Th  
               
               
                 #r Phe Phe Ile Asn Ala  
               
               
                     2050              
               
               
                 #   2055               
               
               
                 #  2060  
               
               
                   
               
               
                 Tyr Thr Thr Gly Pro Cys Thr Pro Leu Pro Al  
               
               
                 #a Pro Asn Tyr Lys Phe  
               
               
                 2065                2070  
               
               
                 #                2075  
               
               
                 #               2080  
               
               
                   
               
               
                 Ala Leu Trp Arg Val Ser Ala Glu Glu Tyr Va  
               
               
                 #l Glu Ile Arg Arg Val  
               
               
                                 2085  
               
               
                 #               2090   
               
               
                 #              2095  
               
               
                   
               
               
                 Gly Asp Phe His Tyr Val Ser Gly Met Thr Th  
               
               
                 #r Asp Asn Leu Lys Cys  
               
               
                             2100      
               
               
                 #           2105       
               
               
                 #          2110  
               
               
                   
               
               
                 Pro Cys Gln Ile Pro Ser Pro Glu Phe Phe Th  
               
               
                 #r Glu Leu Asp Gly Val  
               
               
                         2115          
               
               
                 #       2120           
               
               
                 #      2125  
               
               
                   
               
               
                 Arg Leu His Arg Phe Ala Pro Pro Cys Lys Pr  
               
               
                 #o Leu Leu Arg Glu Glu  
               
               
                     2130              
               
               
                 #   2135               
               
               
                 #  2140  
               
               
                   
               
               
                 Val Ser Phe Arg Val Gly Leu His Glu Tyr Pr  
               
               
                 #o Val Gly Ser Gln Leu  
               
               
                 2145                2150  
               
               
                 #                2155  
               
               
                 #               2160  
               
               
                   
               
               
                 Pro Cys Glu Pro Glu Pro Asp Val Ala Val Le  
               
               
                 #u Thr Ser Met Leu Thr  
               
               
                                 2165  
               
               
                 #               2170   
               
               
                 #              2175  
               
               
                   
               
               
                 Asp Pro Ser His Ile Thr Ala Glu Ala Ala Gl  
               
               
                 #y Arg Arg Leu Ala Arg  
               
               
                             2180      
               
               
                 #           2185       
               
               
                 #          2190  
               
               
                   
               
               
                 Gly Ser Pro Pro Ser Met Ala Ser Ser Ser Al  
               
               
                 #a Ser Gln Leu Ser Ala  
               
               
                         2195          
               
               
                 #       2200           
               
               
                 #      2205  
               
               
                   
               
               
                 Pro Ser Leu Lys Ala Thr Cys Thr Ala Asn Hi  
               
               
                 #s Asp Ser Pro Asp Ala  
               
               
                     2210              
               
               
                 #   2215               
               
               
                 #  2220  
               
               
                   
               
               
                 Glu Leu Ile Glu Ala Asn Leu Leu Trp Arg Gl  
               
               
                 #n Glu Met Gly Gly Asn  
               
               
                 2225                2230  
               
               
                 #                2235  
               
               
                 #               2240  
               
               
                   
               
               
                 Ile Thr Arg Val Glu Ser Glu Asn Lys Val Va  
               
               
                 #l Ile Leu Asp Ser Phe  
               
               
                                 2245  
               
               
                 #               2250   
               
               
                 #              2255  
               
               
                   
               
               
                 Asp Pro Leu Val Ala Glu Glu Asp Glu Arg Gl  
               
               
                 #u Val Ser Val Pro Ala  
               
               
                             2260      
               
               
                 #           2265       
               
               
                 #          2270  
               
               
                   
               
               
                 Glu Ile Leu Arg Lys Ser Arg Arg Phe Ala Pr  
               
               
                 #o Ala Leu Pro Val Trp  
               
               
                         2275          
               
               
                 #       2280           
               
               
                 #      2285  
               
               
                   
               
               
                 Ala Arg Pro Asp Tyr Asn Pro Leu Leu Val Gl  
               
               
                 #u Thr Trp Lys Lys Pro  
               
               
                     2290              
               
               
                 #   2295               
               
               
                 #  2300  
               
               
                   
               
               
                 Asp Tyr Glu Pro Pro Val Val His Gly Cys Pr  
               
               
                 #o Leu Pro Pro Pro Arg  
               
               
                 2305                2310  
               
               
                 #                2315  
               
               
                 #               2320  
               
               
                   
               
               
                 Ser Pro Pro Val Pro Pro Pro Arg Lys Lys Ar  
               
               
                 #g Thr Val Val Leu Thr  
               
               
                                 2325  
               
               
                 #               2330   
               
               
                 #              2335  
               
               
                   
               
               
                 Glu Ser Thr Leu Pro Thr Ala Leu Ala Glu Le  
               
               
                 #u Ala Thr Lys Ser Phe  
               
               
                             2340      
               
               
                 #           2345       
               
               
                 #          2350  
               
               
                   
               
               
                 Gly Ser Ser Ser Thr Ser Gly Ile Thr Gly As  
               
               
                 #p Asn Thr Thr Thr Ser  
               
               
                         2355          
               
               
                 #       2360           
               
               
                 #      2365  
               
               
                   
               
               
                 Ser Glu Pro Ala Pro Ser Gly Cys Pro Pro As  
               
               
                 #p Ser Asp Val Glu Ser  
               
               
                     2370              
               
               
                 #   2375               
               
               
                 #  2380  
               
               
                   
               
               
                 Tyr Ser Ser Met Pro Pro Leu Glu Gly Glu Pr  
               
               
                 #o Gly Asp Pro Asp Leu  
               
               
                 2385                2390  
               
               
                 #                2395  
               
               
                 #               2400  
               
               
                   
               
               
                 Ser Asp Gly Ser Trp Ser Thr Val Ser Ser Gl  
               
               
                 #y Ala Asp Thr Glu Asp  
               
               
                                 2405  
               
               
                 #               2410   
               
               
                 #              2415  
               
               
                   
               
               
                 Val Val Cys Cys Ser Met Ser Tyr Ser Trp Th  
               
               
                 #r Gly Ala Leu Val Thr  
               
               
                             2420      
               
               
                 #           2425       
               
               
                 #          2430  
               
               
                   
               
               
                 Pro Cys Ala Ala Glu Glu Gln Lys Leu Pro Il  
               
               
                 #e Asn Ala Leu Ser Asn  
               
               
                         2435          
               
               
                 #       2440           
               
               
                 #      2445  
               
               
                   
               
               
                 Ser Leu Leu Arg His His Asn Leu Val Tyr Se  
               
               
                 #r Thr Thr Ser Arg Ser  
               
               
                     2450              
               
               
                 #   2455               
               
               
                 #  2460  
               
               
                   
               
               
                 Ala Cys Gln Arg Lys Lys Lys Val Thr Phe As  
               
               
                 #p Arg Leu Gln Val Leu  
               
               
                 2465                2470  
               
               
                 #                2475  
               
               
                 #               2480  
               
               
                   
               
               
                 Asp Ser His Tyr Gln Asp Val Leu Lys Glu Va  
               
               
                 #l Lys Ala Ala Ala Ser  
               
               
                                 2485  
               
               
                 #               2490   
               
               
                 #              2495  
               
               
                   
               
               
                 Lys Val Lys Ala Asn Leu Leu Ser Val Glu Gl  
               
               
                 #u Ala Cys Ser Leu Ala  
               
               
                             2500      
               
               
                 #           2505       
               
               
                 #          2510  
               
               
                   
               
               
                 Pro Pro His Ser Ala Lys Ser Lys Phe Gly Ty  
               
               
                 #r Gly Ala Lys Asp Val  
               
               
                         2515          
               
               
                 #       2520           
               
               
                 #      2525  
               
               
                   
               
               
                 Arg Cys His Ala Arg Lys Ala Val Ala His Il  
               
               
                 #e Asn Ser Val Trp Lys  
               
               
                     2530              
               
               
                 #   2535               
               
               
                 #  2540  
               
               
                   
               
               
                 Asp Leu Leu Glu Asp Ser Val Thr Pro Ile As  
               
               
                 #p Thr Thr Ile Met Ala  
               
               
                 2545                2550  
               
               
                 #                2555  
               
               
                 #               2560  
               
               
                   
               
               
                 Lys Asn Glu Val Phe Cys Val Gln Pro Glu Ly  
               
               
                 #s Gly Gly Arg Lys Pro  
               
               
                                 2565  
               
               
                 #               2570   
               
               
                 #              2575  
               
               
                   
               
               
                 Ala Arg Leu Ile Val Phe Pro Asp Leu Gly Va  
               
               
                 #l Arg Val Cys Glu Lys  
               
               
                             2580      
               
               
                 #           2585       
               
               
                 #          2590  
               
               
                   
               
               
                 Met Ala Leu Tyr Asp Val Val Ser Lys Leu Pr  
               
               
                 #o Leu Ala Val Met Gly  
               
               
                         2595          
               
               
                 #       2600           
               
               
                 #      2605  
               
               
                   
               
               
                 Ser Ser Tyr Gly Phe Gln Tyr Ser Pro Gly Gl  
               
               
                 #n Arg Val Glu Phe Leu  
               
               
                     2610              
               
               
                 #   2615               
               
               
                 #  2620  
               
               
                   
               
               
                 Val Gln Ala Trp Lys Ser Lys Lys Thr Pro Me  
               
               
                 #t Gly Leu Ser Tyr Asp  
               
               
                 2625                2630  
               
               
                 #                2635  
               
               
                 #               2640  
               
               
                   
               
               
                 Thr Arg Cys Phe Asp Ser Thr Val Thr Glu Se  
               
               
                 #r Asp Ile Arg Thr Glu  
               
               
                                 2645  
               
               
                 #               2650   
               
               
                 #              2655  
               
               
                   
               
               
                 Glu Ala Ile Tyr Gln Cys Cys Asp Leu Asp Pr  
               
               
                 #o Gln Ala Arg Val Ala  
               
               
                             2660      
               
               
                 #           2665       
               
               
                 #          2670  
               
               
                   
               
               
                 Ile Lys Ser Leu Thr Glu Arg Leu Tyr Val Gl  
               
               
                 #y Gly Pro Leu Thr Asn  
               
               
                         2675          
               
               
                 #       2680           
               
               
                 #      2685  
               
               
                   
               
               
                 Ser Arg Gly Glu Asn Cys Gly Tyr Arg Arg Cy  
               
               
                 #s Arg Ala Ser Arg Val  
               
               
                     2690              
               
               
                 #   2695               
               
               
                 #  2700  
               
               
                   
               
               
                 Leu Thr Thr Ser Cys Gly Asn Thr Leu Thr Ar  
               
               
                 #g Tyr Ile Lys Ala Arg  
               
               
                 2705                2710  
               
               
                 #                2715  
               
               
                 #               2720  
               
               
                   
               
               
                 Ala Ala Cys Arg Ala Ala Gly Leu Gln Asp Cy  
               
               
                 #s Thr Met Leu Val Cys  
               
               
                                 2725  
               
               
                 #               2730   
               
               
                 #              2735  
               
               
                   
               
               
                 Gly Asp Asp Leu Val Val Ile Cys Glu Ser Al  
               
               
                 #a Gly Val Gln Glu Asp  
               
               
                             2740      
               
               
                 #           2745       
               
               
                 #          2750  
               
               
                   
               
               
                 Ala Ala Ser Leu Arg Ala Phe Thr Glu Ala Me  
               
               
                 #t Thr Arg Tyr Ser Ala  
               
               
                         2755          
               
               
                 #       2760           
               
               
                 #      2765  
               
               
                   
               
               
                 Pro Pro Gly Asp Pro Pro Gln Pro Glu Tyr As  
               
               
                 #p Leu Glu Leu Ile Thr  
               
               
                     2770              
               
               
                 #   2775               
               
               
                 #  2780  
               
               
                   
               
               
                 Ser Cys Ser Ser Asn Val Ser Val Ala His As  
               
               
                 #p Gly Ala Gly Lys Arg  
               
               
                 2785                2790  
               
               
                 #                2795  
               
               
                 #               2800  
               
               
                   
               
               
                 Val Tyr Tyr Leu Thr Arg Asp Pro Thr Thr Pr  
               
               
                 #o Leu Ala Arg Ala Ala  
               
               
                                 2805  
               
               
                 #               2810   
               
               
                 #              2815  
               
               
                   
               
               
                 Trp Glu Thr Ala Arg His Thr Pro Val Asn Se  
               
               
                 #r Trp Leu Gly Asn Ile  
               
               
                             2820      
               
               
                 #           2825       
               
               
                 #          2830  
               
               
                   
               
               
                 Ile Met Phe Ala Pro Thr Leu Trp Ala Arg Me  
               
               
                 #t Ile Leu Met Thr His  
               
               
                         2835          
               
               
                 #       2840           
               
               
                 #      2845  
               
               
                   
               
               
                 Phe Phe Ser Val Leu Ile Ala Arg Asp Gln Le  
               
               
                 #u Glu Gln Ala Leu Asn  
               
               
                     2850              
               
               
                 #   2855               
               
               
                 #  2860  
               
               
                   
               
               
                 Cys Glu Ile Tyr Gly Ala Cys Tyr Ser Ile Gl  
               
               
                 #u Pro Leu Asp Leu Pro  
               
               
                 2865                2870  
               
               
                 #                2875  
               
               
                 #               2880  
               
               
                   
               
               
                 Pro Ile Ile Gln Arg Leu His Gly Leu Ser Al  
               
               
                 #a Phe Ser Leu His Ser  
               
               
                                 2885  
               
               
                 #               2890   
               
               
                 #              2895  
               
               
                   
               
               
                 Tyr Ser Pro Gly Glu Ile Asn Arg Val Ala Al  
               
               
                 #a Cys Leu Arg Lys Leu  
               
               
                             2900      
               
               
                 #           2905       
               
               
                 #          2910  
               
               
                   
               
               
                 Gly Val Pro Pro Leu Arg Ala Trp Arg His Ar  
               
               
                 #g Ala Trp Ser Val Arg  
               
               
                         2915          
               
               
                 #       2920           
               
               
                 #      2925  
               
               
                   
               
               
                 Ala Arg Leu Leu Ala Arg Gly Gly Lys Ala Al  
               
               
                 #a Ile Cys Gly Lys Tyr  
               
               
                     2930              
               
               
                 #   2935               
               
               
                 #  2940  
               
               
                   
               
               
                 Leu Phe Asn Trp Ala Val Arg Thr Lys Leu Ly  
               
               
                 #s Leu Thr Pro Ile Thr  
               
               
                 2945                2950  
               
               
                 #                2955  
               
               
                 #               2960  
               
               
                   
               
               
                 Ala Ala Gly Arg Leu Asp Leu Ser Gly Trp Ph  
               
               
                 #e Thr Ala Gly Tyr Ser  
               
               
                                 2965  
               
               
                 #               2970   
               
               
                 #              2975  
               
               
                   
               
               
                 Gly Gly Asp Ile Tyr His Ser Val Ser His Al  
               
               
                 #a Arg Pro Arg Trp Phe  
               
               
                             2980      
               
               
                 #           2985       
               
               
                 #          2990  
               
               
                   
               
               
                 Trp Phe Cys Leu Leu Leu Leu Ala Ala Gly Va  
               
               
                 #l Gly Ile Tyr Leu Leu  
               
               
                         2995          
               
               
                 #       3000           
               
               
                 #      3005  
               
               
                   
               
               
                 Pro Asn Arg  
               
               
                     3010  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 2  
               
               
                 &lt;211&gt; LENGTH: 182  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus core  
               
               
                 #protein sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 2  
               
               
                   
               
               
                 Met Ser Thr Asn Pro Lys Pro Gln Arg Lys Th  
               
               
                 #r Lys Arg Asn Thr Asn  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Arg Arg Pro Gln Asp Val Lys Phe Pro Gly Gl  
               
               
                 #y Gly Gln Ile Val Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Gly Val Tyr Leu Leu Pro Arg Arg Gly Pro Ar  
               
               
                 #g Leu Gly Val Arg Ala  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Thr Arg Lys Thr Ser Glu Arg Ser Gln Pro Ar  
               
               
                 #g Gly Arg Arg Gln Pro  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Ile Pro Lys Ala Arg Arg Pro Glu Gly Arg Th  
               
               
                 #r Trp Ala Gln Pro Gly  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Tyr Pro Trp Pro Leu Tyr Gly Asn Glu Gly Cy  
               
               
                 #s Gly Trp Ala Gly Trp  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Leu Leu Ser Pro Arg Gly Ser Arg Pro Ser Tr  
               
               
                 #p Gly Pro Thr Asp Pro  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Arg Arg Arg Ser Arg Asn Leu Gly Lys Val Il  
               
               
                 #e Asp Thr Leu Thr Cys  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Gly Phe Ala Asp Leu Met Gly Tyr Ile Pro Le  
               
               
                 #u Val Gly Ala Pro Leu  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Gly Gly Ala Ala Arg Ala Leu Ala His Gly Va  
               
               
                 #l Arg Val Leu Glu Asp  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Gly Val Asn Tyr Ala Thr Gly Asn Leu Pro Gl  
               
               
                 #y Cys Ser Phe Ser Ile  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Phe Leu Leu Ala Leu Leu  
               
               
                             180  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 3  
               
               
                 &lt;211&gt; LENGTH: 197  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus E1 p  
               
               
                 #rotein sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 3  
               
               
                   
               
               
                 Ser Cys Leu Thr Val Pro Ala Ser Ala Tyr Gl  
               
               
                 #n Val Arg Asn Ser Ser  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Gly Leu Tyr His Val Thr Asn Asp Cys Pro As  
               
               
                 #n Ser Ser Val Val Tyr  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Glu Ala Ala Asp Ala Ile Leu His Thr Pro Gl  
               
               
                 #y Cys Val Pro Cys Val  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Arg Glu Gly Asn Ala Ser Arg Cys Trp Val Al  
               
               
                 #a Val Thr Pro Thr Val  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Ala Thr Arg Asp Gly Lys Leu Pro Thr Thr Gl  
               
               
                 #n Leu Arg Arg His Ile  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Asp Leu Leu Val Gly Ser Ala Thr Leu Cys Se  
               
               
                 #r Ala Leu Tyr Val Gly  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Asp Leu Cys Gly Ser Val Phe Leu Val Gly Gl  
               
               
                 #n Leu Phe Thr Phe Ser  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Pro Arg His His Trp Thr Thr Gln Asp Cys As  
               
               
                 #n Cys Ser Ile Tyr Pro  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Gly His Ile Thr Gly His Arg Met Ala Trp As  
               
               
                 #n Met Met Met Asn Trp  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ser Pro Thr Ala Ala Leu Val Val Ala Gln Le  
               
               
                 #u Leu Arg Ile Pro Gln  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Ala Ile Met Asp Met Ile Ala Gly Ala His Tr  
               
               
                 #p Gly Val Leu Ala Gly  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Ile Lys Tyr Phe Ser Met Val Gly Asn Trp Al  
               
               
                 #a Lys Val Leu Val Val  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Leu Leu Leu Phe Ala  
               
               
                         195  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 4  
               
               
                 &lt;211&gt; LENGTH: 350  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus E2 p  
               
               
                 #rotein sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 4  
               
               
                   
               
               
                 Gly Val Asp Ala Glu Thr His Val Thr Gly Gl  
               
               
                 #y Asn Ala Gly Arg Thr  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Thr Ala Gly Leu Val Gly Leu Leu Thr Pro Gl  
               
               
                 #y Ala Lys Gln Asn Ile  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Gln Leu Ile Asn Thr Asn Gly Ser Trp His Il  
               
               
                 #e Asn Ser Thr Ala Leu  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Asn Cys Asn Glu Ser Leu Asn Thr Gly Trp Le  
               
               
                 #u Ala Gly Leu Phe Tyr  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Gln His Lys Phe Asn Ser Ser Gly Cys Pro Gl  
               
               
                 #u Arg Leu Ala Ser Cys  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Arg Arg Leu Thr Asp Phe Ala Gln Gly Trp Gl  
               
               
                 #y Pro Ile Ser Tyr Ala  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Asn Gly Ser Gly Leu Asp Glu Arg Pro Tyr Cy  
               
               
                 #s Trp His Tyr Pro Pro  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Arg Pro Cys Gly Ile Val Pro Ala Lys Ser Va  
               
               
                 #l Cys Gly Pro Val Tyr  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Cys Phe Thr Pro Ser Pro Val Val Val Gly Th  
               
               
                 #r Thr Asp Arg Ser Gly  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ala Pro Thr Tyr Ser Trp Gly Ala Asn Asp Th  
               
               
                 #r Asp Val Phe Val Leu  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Asn Asn Thr Arg Pro Pro Leu Gly Asn Trp Ph  
               
               
                 #e Gly Cys Thr Trp Met  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Asn Ser Thr Gly Phe Thr Lys Val Cys Gly Al  
               
               
                 #a Pro Pro Cys Val Ile  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Gly Gly Val Gly Asn Asn Thr Leu Leu Cys Pr  
               
               
                 #o Thr Asp Cys Phe Arg  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Lys Tyr Pro Glu Ala Thr Tyr Ser Arg Cys Gl  
               
               
                 #y Ser Gly Pro Arg Ile  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Thr Pro Arg Cys Met Val Asp Tyr Pro Tyr Ar  
               
               
                 #g Leu Trp His Tyr Pro  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Cys Thr Ile Asn Tyr Thr Ile Phe Lys Val Ar  
               
               
                 #g Met Tyr Val Gly Gly  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Val Glu His Arg Leu Glu Ala Ala Cys Asn Tr  
               
               
                 #p Thr Arg Gly Glu Arg  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Cys Asp Leu Glu Asp Arg Asp Arg Ser Glu Le  
               
               
                 #u Ser Pro Leu Leu Leu  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Ser Thr Thr Gln Trp Gln Val Leu Pro Cys Se  
               
               
                 #r Phe Thr Thr Leu Pro  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Ala Leu Ser Thr Gly Leu Ile His Leu His Gl  
               
               
                 #n Asn Ile Val Asp Val  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Gln Tyr Leu Tyr Gly Val Gly Ser Ser Ile Al  
               
               
                 #a Ser Trp Ala Ile Lys  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Trp Glu Tyr Val Val Leu Leu Phe Leu Leu Le  
               
               
                 #u Ala Asp Ala  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 5  
               
               
                 &lt;211&gt; LENGTH: 315  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus NS2  
               
               
                 #protein sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 5  
               
               
                   
               
               
                 Arg Val Cys Ser Cys Leu Trp Met Met Leu Le  
               
               
                 #u Ile Ser Gln Ala Glu  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Ala Ala Leu Glu Asn Leu Val Ile Leu Asn Al  
               
               
                 #a Ala Ser Leu Ala Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Thr His Gly Leu Val Ser Phe Leu Val Phe Ph  
               
               
                 #e Cys Phe Ala Trp Tyr  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Leu Lys Gly Arg Trp Val Pro Gly Ala Val Ty  
               
               
                 #r Ala Leu Tyr Gly Met  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Trp Pro Leu Leu Leu Leu Leu Leu Ala Leu Pr  
               
               
                 #o Gln Arg Ala Tyr Ala  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Leu Asp Thr Glu Val Ala Ala Ser Cys Gly Gl  
               
               
                 #y Val Val Leu Val Gly  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Leu Met Ala Leu Thr Leu Ser Pro Tyr Tyr Ly  
               
               
                 #s Arg Tyr Ile Ser Trp  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Cys Met Trp Trp Leu Gln Tyr Phe Leu Thr Ar  
               
               
                 #g Val Glu Ala Gln Leu  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 His Val Trp Val Pro Pro Leu Asn Val Arg Gl  
               
               
                 #y Gly Arg Asp Ala Val  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ile Leu Leu Thr Cys Val Val His Pro Ala Le  
               
               
                 #u Val Phe Asp Ile Thr  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Lys Leu Leu Leu Ala Ile Phe Gly Pro Leu Tr  
               
               
                 #p Ile Leu Gln Ala Ser  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Leu Leu Lys Val Pro Tyr Phe Val Arg Val Gl  
               
               
                 #n Gly Leu Leu Arg Ile  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Cys Ala Leu Ala Arg Lys Ile Ala Gly Gly Hi  
               
               
                 #s Tyr Val Gln Met Ala  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Ile Ile Lys Leu Gly Ala Leu Thr Gly Thr Cy  
               
               
                 #s Val Tyr Asn His Leu  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Ala Pro Leu Arg Asp Trp Ala His Asn Gly Le  
               
               
                 #u Arg Asp Leu Ala Val  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Ala Val Glu Pro Val Val Phe Ser Arg Met Gl  
               
               
                 #u Thr Lys Leu Ile Thr  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Trp Gly Ala Asp Thr Ala Ala Cys Gly Asp Il  
               
               
                 #e Ile Asn Gly Leu Pro  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Val Ser Ala Arg Arg Gly Gln Glu Ile Leu Le  
               
               
                 #u Gly Pro Ala Asp Gly  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Met Val Ser Lys Gly Trp Arg Leu Leu Ala Pr  
               
               
                 #o Ile Thr Ala Tyr Ala  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Gln Gln Thr Arg Gly Leu Leu Gly Cys Ile Il  
               
               
                 #e  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 6  
               
               
                 &lt;211&gt; LENGTH: 613  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus NS3  
               
               
                 #protein sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 6  
               
               
                   
               
               
                 Thr Ser Leu Thr Gly Arg Asp Lys Asn Gln Va  
               
               
                 #l Glu Gly Glu Val Gln  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Ile Val Ser Thr Ala Thr Gln Thr Phe Leu Al  
               
               
                 #a Thr Cys Ile Asn Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Val Cys Trp Thr Val Tyr His Gly Ala Gly Th  
               
               
                 #r Arg Thr Ile Ala Ser  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Pro Lys Gly Pro Val Ile Gln Thr Tyr Thr As  
               
               
                 #n Val Asp Gln Asp Leu  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Val Gly Trp Pro Ala Pro Gln Gly Ser Arg Se  
               
               
                 #r Leu Thr Pro Cys Thr  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Cys Gly Ser Ser Asp Leu Tyr Leu Val Thr Ar  
               
               
                 #g His Ala Asp Val Ile  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Pro Val Arg Arg Arg Gly Asp Ser Arg Gly Se  
               
               
                 #r Leu Leu Ser Pro Arg  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Pro Ile Ser Tyr Leu Lys Gly Ser Ser Gly Gl  
               
               
                 #y Pro Leu Leu Cys Pro  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Thr Gly His Ala Val Gly Leu Phe Arg Ala Al  
               
               
                 #a Val Cys Thr Arg Gly  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Val Ala Lys Ala Val Asp Phe Ile Pro Val Gl  
               
               
                 #u Asn Leu Glu Thr Thr  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Met Arg Ser Pro Val Phe Thr Asp Asn Ser Se  
               
               
                 #r Pro Pro Ala Val Pro  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Gln Ser Phe Gln Val Ala His Leu His Ala Pr  
               
               
                 #o Thr Gly Ser Gly Lys  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Ser Thr Lys Val Pro Ala Ala Tyr Ala Ala Ly  
               
               
                 #s Gly Tyr Lys Val Leu  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Val Leu Asn Pro Ser Val Ala Ala Thr Leu Gl  
               
               
                 #y Phe Gly Ala Tyr Met  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Ser Lys Ala His Gly Val Asp Pro Asn Ile Ar  
               
               
                 #g Thr Gly Val Arg Thr  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Ile Thr Thr Gly Ser Pro Ile Thr Tyr Ser Th  
               
               
                 #r Tyr Gly Lys Phe Leu  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Ala Asp Ala Gly Cys Ser Gly Gly Ala Tyr As  
               
               
                 #p Ile Ile Ile Cys Asp  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Glu Cys His Ser Thr Asp Ala Thr Ser Ile Se  
               
               
                 #r Gly Ile Gly Thr Val  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Leu Asp Gln Ala Glu Thr Ala Gly Ala Arg Le  
               
               
                 #u Val Val Leu Ala Thr  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Ala Thr Pro Pro Gly Ser Val Thr Val Ser Hi  
               
               
                 #s Pro Asn Ile Glu Glu  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Val Ala Leu Ser Thr Thr Gly Glu Ile Pro Ph  
               
               
                 #e Tyr Gly Lys Ala Ile  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Pro Leu Glu Val Ile Lys Gly Gly Arg His Le  
               
               
                 #u Ile Phe Cys His Ser  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Lys Lys Lys Cys Asp Glu Leu Ala Ala Lys Le  
               
               
                 #u Val Ala Leu Gly Ile  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Asn Ala Val Ala Tyr Tyr Arg Gly Leu Asp Va  
               
               
                 #l Ser Val Ile Pro Thr  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Ser Gly Asp Val Val Val Val Ser Thr Asp Al  
               
               
                 #a Leu Met Thr Gly Phe  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Thr Gly Asp Phe Asp Ser Val Ile Asp Cys As  
               
               
                 #n Thr Cys Val Thr Gln  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Thr Val Asp Phe Ser Leu Asp Pro Thr Phe Th  
               
               
                 #r Ile Glu Thr Thr Thr  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Leu Pro Gln Asp Ala Val Ser Arg Thr Gln Ar  
               
               
                 #g Arg Gly Arg Thr Gly  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Arg Gly Lys Pro Gly Ile Tyr Arg Phe Val Al  
               
               
                 #a Pro Gly Glu Arg Pro  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Ser Gly Met Phe Asp Ser Ser Val Leu Cys Gl  
               
               
                 #u Cys Tyr Asp Ala Gly  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Cys Ala Trp Tyr Glu Leu Thr Pro Ala Glu Th  
               
               
                 #r Thr Val Arg Leu Arg  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Ala Tyr Met Asn Thr Pro Gly Leu Pro Val Cy  
               
               
                 #s Gln Asp His Leu Gly  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Phe Trp Glu Gly Val Phe Thr Gly Leu Thr Hi  
               
               
                 #s Ile Asp Ala His Phe  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Leu Ser Gln Thr Lys Gln Ser Gly Glu Asn Ph  
               
               
                 #e Pro Tyr Leu Val Ala  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Tyr Gln Ala Thr Val Cys Ala Arg Ala Gln Al  
               
               
                 #a Pro Pro Pro Ser Trp  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Asp Gln Met Arg Lys Cys Leu Ile Arg Leu Ly  
               
               
                 #s Pro Thr Leu His Gly  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Pro Thr Pro Leu Leu Tyr Arg Leu Gly Ala Va  
               
               
                 #l Gln Asn Glu Val Thr  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Leu Thr His Pro Ile Thr Lys Tyr Ile Met Th  
               
               
                 #r Cys Met Ser Ala Asp  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Leu Glu Val Val Thr  
               
               
                     610  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 7  
               
               
                 &lt;211&gt; LENGTH: 54  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus NS4A  
               
               
                 #protein sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 7  
               
               
                   
               
               
                 Ser Thr Trp Val Leu Val Gly Gly Val Leu Al  
               
               
                 #a Ala Leu Ala Ala Tyr  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Cys Leu Ser Thr Gly Cys Val Val Ile Val Gl  
               
               
                 #y Arg Ile Val Leu Ser  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Gly Lys Pro Ala Ile Ile Pro Asp Arg Glu Va  
               
               
                 #l Leu Tyr Gln Glu Phe  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Asp Glu Met Glu Glu Cys  
               
               
                     50  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 8  
               
               
                 &lt;211&gt; LENGTH: 260  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus NS4B  
               
               
                 #protein sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 8  
               
               
                   
               
               
                 Ser Gln His Leu Pro Tyr Ile Glu Gln Gly Me  
               
               
                 #t Met Leu Ala Glu Gln  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Phe Lys Gln Lys Ala Leu Gly Leu Leu Gln Th  
               
               
                 #r Ala Ser Arg His Ala  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Glu Val Ile Thr Pro Ala Val Gln Thr Asn Tr  
               
               
                 #p Gln Lys Leu Glu Val  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Phe Trp Ala Lys His Met Trp Asn Phe Ile Se  
               
               
                 #r Gly Ile Gln Tyr Leu  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Ala Gly Leu Ser Thr Leu Pro Gly Asn Pro Al  
               
               
                 #a Ile Ala Ser Leu Met  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Ala Phe Thr Ala Ala Val Thr Ser Pro Leu Th  
               
               
                 #r Thr Gly Gln Thr Leu  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Leu Phe Asn Ile Leu Gly Gly Trp Val Ala Al  
               
               
                 #a Gln Leu Ala Ala Pro  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Gly Ala Ala Thr Ala Phe Val Gly Ala Gly Le  
               
               
                 #u Ala Gly Ala Ala Leu  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Asp Ser Val Gly Leu Gly Lys Val Leu Val As  
               
               
                 #p Ile Leu Ala Gly Tyr  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Gly Ala Gly Val Ala Gly Ala Leu Val Ala Ph  
               
               
                 #e Lys Ile Met Ser Gly  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Glu Val Pro Ser Thr Glu Asp Leu Val Asn Le  
               
               
                 #u Leu Pro Ala Ile Leu  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Ser Pro Gly Ala Leu Ala Val Gly Val Val Ph  
               
               
                 #e Ala Ser Ile Leu Arg  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Arg Arg Val Gly Pro Gly Glu Gly Ala Val Gl  
               
               
                 #n Trp Met Asn Arg Leu  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Ile Ala Phe Ala Ser Arg Gly Asn His Val Se  
               
               
                 #r Pro Thr His Tyr Val  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Pro Glu Ser Asp Ala Ala Ala Arg Val Thr Al  
               
               
                 #a Ile Leu Ser Ser Leu  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Thr Val Thr Gln Leu Leu Arg Arg Leu His Gl  
               
               
                 #n Trp Ile Ser Ser Glu  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Cys Thr Thr Pro  
               
               
                             260  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 9  
               
               
                 &lt;211&gt; LENGTH: 1040  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus NS5A/B  
               
               
                 #protein sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 9  
               
               
                   
               
               
                 Cys Ser Gly Ser Trp Leu Arg Asp Ile Trp As  
               
               
                 #p Trp Ile Cys Glu Val  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Leu Ser Asp Phe Lys Thr Trp Leu Lys Ala Ly  
               
               
                 #s Leu Met Pro Gln Leu  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Pro Gly Ile Pro Phe Val Ser Cys Gln Arg Gl  
               
               
                 #y Tyr Arg Gly Val Trp  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Arg Gly Asp Gly Ile Met His Thr Arg Cys Hi  
               
               
                 #s Cys Gly Ala Glu Ile  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Thr Gly His Val Lys Asn Gly Thr Met Arg Il  
               
               
                 #e Val Gly Pro Arg Thr  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Cys Lys Asn Met Trp Ser Gly Thr Phe Phe Il  
               
               
                 #e Asn Ala Tyr Thr Thr  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Gly Pro Cys Thr Pro Leu Pro Ala Pro Asn Ty  
               
               
                 #r Lys Phe Ala Leu Trp  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Arg Val Ser Ala Glu Glu Tyr Val Glu Ile Ar  
               
               
                 #g Arg Val Gly Asp Phe  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 His Tyr Val Ser Gly Met Thr Thr Asp Asn Le  
               
               
                 #u Lys Cys Pro Cys Gln  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ile Pro Ser Pro Glu Phe Phe Thr Glu Leu As  
               
               
                 #p Gly Val Arg Leu His  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Arg Phe Ala Pro Pro Cys Lys Pro Leu Leu Ar  
               
               
                 #g Glu Glu Val Ser Phe  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Arg Val Gly Leu His Glu Tyr Pro Val Gly Se  
               
               
                 #r Gln Leu Pro Cys Glu  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Pro Glu Pro Asp Val Ala Val Leu Thr Ser Me  
               
               
                 #t Leu Thr Asp Pro Ser  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 His Ile Thr Ala Glu Ala Ala Gly Arg Arg Le  
               
               
                 #u Ala Arg Gly Ser Pro  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Pro Ser Met Ala Ser Ser Ser Ala Ser Gln Le  
               
               
                 #u Ser Ala Pro Ser Leu  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Lys Ala Thr Cys Thr Ala Asn His Asp Ser Pr  
               
               
                 #o Asp Ala Glu Leu Ile  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Glu Ala Asn Leu Leu Trp Arg Gln Glu Met Gl  
               
               
                 #y Gly Asn Ile Thr Arg  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Val Glu Ser Glu Asn Lys Val Val Ile Leu As  
               
               
                 #p Ser Phe Asp Pro Leu  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Val Ala Glu Glu Asp Glu Arg Glu Val Ser Va  
               
               
                 #l Pro Ala Glu Ile Leu  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Arg Lys Ser Arg Arg Phe Ala Pro Ala Leu Pr  
               
               
                 #o Val Trp Ala Arg Pro  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Asp Tyr Asn Pro Leu Leu Val Glu Thr Trp Ly  
               
               
                 #s Lys Pro Asp Tyr Glu  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Pro Pro Val Val His Gly Cys Pro Leu Pro Pr  
               
               
                 #o Pro Arg Ser Pro Pro  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Val Pro Pro Pro Arg Lys Lys Arg Thr Val Va  
               
               
                 #l Leu Thr Glu Ser Thr  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Leu Pro Thr Ala Leu Ala Glu Leu Ala Thr Ly  
               
               
                 #s Ser Phe Gly Ser Ser  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Ser Thr Ser Gly Ile Thr Gly Asp Asn Thr Th  
               
               
                 #r Thr Ser Ser Glu Pro  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Ala Pro Ser Gly Cys Pro Pro Asp Ser Asp Va  
               
               
                 #l Glu Ser Tyr Ser Ser  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Met Pro Pro Leu Glu Gly Glu Pro Gly Asp Pr  
               
               
                 #o Asp Leu Ser Asp Gly  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Ser Trp Ser Thr Val Ser Ser Gly Ala Asp Th  
               
               
                 #r Glu Asp Val Val Cys  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Cys Ser Met Ser Tyr Ser Trp Thr Gly Ala Le  
               
               
                 #u Val Thr Pro Cys Ala  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Ala Glu Glu Gln Lys Leu Pro Ile Asn Ala Le  
               
               
                 #u Ser Asn Ser Leu Leu  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Arg His His Asn Leu Val Tyr Ser Thr Thr Se  
               
               
                 #r Arg Ser Ala Cys Gln  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Arg Lys Lys Lys Val Thr Phe Asp Arg Leu Gl  
               
               
                 #n Val Leu Asp Ser His  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Tyr Gln Asp Val Leu Lys Glu Val Lys Ala Al  
               
               
                 #a Ala Ser Lys Val Lys  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Ala Asn Leu Leu Ser Val Glu Glu Ala Cys Se  
               
               
                 #r Leu Ala Pro Pro His  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Ser Ala Lys Ser Lys Phe Gly Tyr Gly Ala Ly  
               
               
                 #s Asp Val Arg Cys His  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Ala Arg Lys Ala Val Ala His Ile Asn Ser Va  
               
               
                 #l Trp Lys Asp Leu Leu  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Glu Asp Ser Val Thr Pro Ile Asp Thr Thr Il  
               
               
                 #e Met Ala Lys Asn Glu  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Val Phe Cys Val Gln Pro Glu Lys Gly Gly Ar  
               
               
                 #g Lys Pro Ala Arg Leu  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Ile Val Phe Pro Asp Leu Gly Val Arg Val Cy  
               
               
                 #s Glu Lys Met Ala Leu  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Tyr Asp Val Val Ser Lys Leu Pro Leu Ala Va  
               
               
                 #l Met Gly Ser Ser Tyr  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Gly Phe Gln Tyr Ser Pro Gly Gln Arg Val Gl  
               
               
                 #u Phe Leu Val Gln Ala  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Trp Lys Ser Lys Lys Thr Pro Met Gly Leu Se  
               
               
                 #r Tyr Asp Thr Arg Cys  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Phe Asp Ser Thr Val Thr Glu Ser Asp Ile Ar  
               
               
                 #g Thr Glu Glu Ala Ile  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Tyr Gln Cys Cys Asp Leu Asp Pro Gln Ala Ar  
               
               
                 #g Val Ala Ile Lys Ser  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Leu Thr Glu Arg Leu Tyr Val Gly Gly Pro Le  
               
               
                 #u Thr Asn Ser Arg Gly  
               
               
                 705                 7  
               
               
                 #10                 7  
               
               
                 #15                 7  
               
               
                 #20  
               
               
                   
               
               
                 Glu Asn Cys Gly Tyr Arg Arg Cys Arg Ala Se  
               
               
                 #r Arg Val Leu Thr Thr  
               
               
                                 725   
               
               
                 #               730   
               
               
                 #               735  
               
               
                   
               
               
                 Ser Cys Gly Asn Thr Leu Thr Arg Tyr Ile Ly  
               
               
                 #s Ala Arg Ala Ala Cys  
               
               
                             740       
               
               
                 #           745       
               
               
                 #           750  
               
               
                   
               
               
                 Arg Ala Ala Gly Leu Gln Asp Cys Thr Met Le  
               
               
                 #u Val Cys Gly Asp Asp  
               
               
                         755           
               
               
                 #       760           
               
               
                 #       765  
               
               
                   
               
               
                 Leu Val Val Ile Cys Glu Ser Ala Gly Val Gl  
               
               
                 #n Glu Asp Ala Ala Ser  
               
               
                     770               
               
               
                 #   775               
               
               
                 #   780  
               
               
                   
               
               
                 Leu Arg Ala Phe Thr Glu Ala Met Thr Arg Ty  
               
               
                 #r Ser Ala Pro Pro Gly  
               
               
                 785                 7  
               
               
                 #90                 7  
               
               
                 #95                 8  
               
               
                 #00  
               
               
                   
               
               
                 Asp Pro Pro Gln Pro Glu Tyr Asp Leu Glu Le  
               
               
                 #u Ile Thr Ser Cys Ser  
               
               
                                 805   
               
               
                 #               810   
               
               
                 #               815  
               
               
                   
               
               
                 Ser Asn Val Ser Val Ala His Asp Gly Ala Gl  
               
               
                 #y Lys Arg Val Tyr Tyr  
               
               
                             820       
               
               
                 #           825       
               
               
                 #           830  
               
               
                   
               
               
                 Leu Thr Arg Asp Pro Thr Thr Pro Leu Ala Ar  
               
               
                 #g Ala Ala Trp Glu Thr  
               
               
                         835           
               
               
                 #       840           
               
               
                 #       845  
               
               
                   
               
               
                 Ala Arg His Thr Pro Val Asn Ser Trp Leu Gl  
               
               
                 #y Asn Ile Ile Met Phe  
               
               
                     850               
               
               
                 #   855               
               
               
                 #   860  
               
               
                   
               
               
                 Ala Pro Thr Leu Trp Ala Arg Met Ile Leu Me  
               
               
                 #t Thr His Phe Phe Ser  
               
               
                 865                 8  
               
               
                 #70                 8  
               
               
                 #75                 8  
               
               
                 #80  
               
               
                   
               
               
                 Val Leu Ile Ala Arg Asp Gln Leu Glu Gln Al  
               
               
                 #a Leu Asn Cys Glu Ile  
               
               
                                 885   
               
               
                 #               890   
               
               
                 #               895  
               
               
                   
               
               
                 Tyr Gly Ala Cys Tyr Ser Ile Glu Pro Leu As  
               
               
                 #p Leu Pro Pro Ile Ile  
               
               
                             900       
               
               
                 #           905       
               
               
                 #           910  
               
               
                   
               
               
                 Gln Arg Leu His Gly Leu Ser Ala Phe Ser Le  
               
               
                 #u His Ser Tyr Ser Pro  
               
               
                         915           
               
               
                 #       920           
               
               
                 #       925  
               
               
                   
               
               
                 Gly Glu Ile Asn Arg Val Ala Ala Cys Leu Ar  
               
               
                 #g Lys Leu Gly Val Pro  
               
               
                     930               
               
               
                 #   935               
               
               
                 #   940  
               
               
                   
               
               
                 Pro Leu Arg Ala Trp Arg His Arg Ala Trp Se  
               
               
                 #r Val Arg Ala Arg Leu  
               
               
                 945                 9  
               
               
                 #50                 9  
               
               
                 #55                 9  
               
               
                 #60  
               
               
                   
               
               
                 Leu Ala Arg Gly Gly Lys Ala Ala Ile Cys Gl  
               
               
                 #y Lys Tyr Leu Phe Asn  
               
               
                                 965   
               
               
                 #               970   
               
               
                 #               975  
               
               
                   
               
               
                 Trp Ala Val Arg Thr Lys Leu Lys Leu Thr Pr  
               
               
                 #o Ile Thr Ala Ala Gly  
               
               
                             980       
               
               
                 #           985       
               
               
                 #           990  
               
               
                   
               
               
                 Arg Leu Asp Leu Ser Gly Trp Phe Thr Ala Gl  
               
               
                 #y Tyr Ser Gly Gly Asp  
               
               
                         995           
               
               
                 #       1000           
               
               
                 #      1005  
               
               
                   
               
               
                 Ile Tyr His Ser Val Ser His Ala Arg Pro Ar  
               
               
                 #g Trp Phe Trp Phe Cys  
               
               
                     1010              
               
               
                 #   1015               
               
               
                 #  1020  
               
               
                   
               
               
                 Leu Leu Leu Leu Ala Ala Gly Val Gly Ile Ty  
               
               
                 #r Leu Leu Pro Asn Arg  
               
               
                 1025                 
               
               
                 #1030                103  
               
               
                 #5                1040  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 10  
               
               
                 &lt;211&gt; LENGTH: 226  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis B virus S an  
               
               
                 #tigen (HBsAg) sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 10  
               
               
                   
               
               
                 Met Glu Asn Ile Thr Ser Gly Phe Leu Gly Pr  
               
               
                 #o Leu Leu Val Leu Gln  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Ala Gly Phe Phe Leu Leu Thr Arg Ile Leu Th  
               
               
                 #r Ile Pro Gln Ser Leu  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Asp Ser Trp Trp Thr Ser Leu Asn Phe Leu Gl  
               
               
                 #y Gly Thr Thr Val Cys  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Leu Gly Gln Asn Ser Gln Ser Pro Thr Ser As  
               
               
                 #n His Ser Pro Thr Ser  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Cys Pro Pro Thr Cys Pro Gly Tyr Arg Trp Me  
               
               
                 #t Cys Leu Arg Arg Phe  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Ile Ile Phe Leu Phe Ile Leu Leu Leu Cys Le  
               
               
                 #u Ile Phe Leu Leu Val  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Leu Leu Asp Tyr Gln Gly Met Leu Pro Val Cy  
               
               
                 #s Pro Leu Ile Pro Gly  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Ser Ser Thr Thr Ser Thr Gly Pro Cys Arg Th  
               
               
                 #r Cys Met Thr Thr Ala  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Gln Gly Thr Ser Met Tyr Pro Ser Cys Cys Cy  
               
               
                 #s Thr Lys Pro Ser Asp  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Gly Asn Cys Thr Cys Ile Pro Ile Pro Ser Se  
               
               
                 #r Trp Ala Phe Gly Lys  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Phe Leu Trp Glu Trp Ala Ser Ala Arg Phe Se  
               
               
                 #r Trp Leu Ser Leu Leu  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Val Pro Phe Val Gln Trp Phe Val Gly Leu Se  
               
               
                 #r Pro Thr Val Trp Leu  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Ser Val Ile Trp Met Met Trp Tyr Trp Gly Pr  
               
               
                 #o Ser Leu Tyr Ser Ile  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Leu Ser Pro Phe Leu Pro Leu Leu Pro Ile Ph  
               
               
                 #e Phe Cys Leu Trp Val  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Tyr Ile  
               
               
                 225  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 11  
               
               
                 &lt;211&gt; LENGTH: 212  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis B virus C an  
               
               
                 #tigen and e antigen  
               
               
                       (HBcAg/HBeAg) sequence  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 11  
               
               
                   
               
               
                 Met Gln Leu Phe His Leu Cys Leu Ile Ile Se  
               
               
                 #r Cys Ser Cys Pro Thr  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Val Gln Ala Ser Lys Leu Cys Leu Gly Trp Le  
               
               
                 #u Trp Gly Met Asp Ile  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Asp Pro Tyr Lys Glu Phe Gly Ala Thr Val Gl  
               
               
                 #u Leu Leu Ser Phe Leu  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Pro Ser Asp Phe Phe Pro Ser Val Arg Asp Le  
               
               
                 #u Leu Asp Thr Ala Ser  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Ala Leu Tyr Arg Glu Ala Leu Glu Ser Pro Gl  
               
               
                 #u His Cys Ser Pro His  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 His Thr Ala Leu Arg Gln Ala Ile Leu Cys Tr  
               
               
                 #p Gly Glu Leu Met Thr  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Leu Ala Thr Trp Val Gly Val Asn Leu Glu As  
               
               
                 #p Pro Ala Ser Arg Asp  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Leu Val Val Ser Tyr Val Asn Thr Asn Met Gl  
               
               
                 #y Leu Lys Phe Arg Gln  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Leu Leu Trp Phe His Ile Ser Cys Leu Thr Ph  
               
               
                 #e Gly Arg Glu Thr Val  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ile Glu Tyr Leu Val Ser Phe Gly Val Trp Il  
               
               
                 #e Arg Thr Pro Pro Ala  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Tyr Arg Pro Pro Asn Ala Pro Ile Leu Ser Th  
               
               
                 #r Leu Pro Glu Thr Thr  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Val Val Arg Arg Arg Gly Arg Ser Pro Arg Ar  
               
               
                 #g Arg Thr Pro Ser Pro  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Arg Arg Arg Arg Ser Gln Ser Pro Arg Arg Ar  
               
               
                 #g Arg Ser Gln Ser Arg  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Glu Ser Gln Cys  
               
               
                     210  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 12  
               
               
                 &lt;211&gt; LENGTH: 2227  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis A virus sequenc  
               
               
                 #e  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 12  
               
               
                   
               
               
                 Met Asn Met Ser Lys Gln Gly Ile Phe Gln Th  
               
               
                 #r Val Gly Ser Gly Leu  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Asp His Ile Leu Ser Leu Ala Asp Ile Glu Gl  
               
               
                 #u Glu Gln Met Ile Gln  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Ser Val Asp Arg Thr Ala Val Thr Gly Ala Se  
               
               
                 #r Tyr Phe Thr Ser Val  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Asp Gln Ser Ser Val His Thr Ala Glu Val Gl  
               
               
                 #y Ser His Gln Ile Glu  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Pro Leu Lys Thr Ser Val Asp Lys Pro Gly Se  
               
               
                 #r Lys Lys Thr Gln Gly  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Glu Lys Phe Phe Leu Ile His Ser Ala Asp Tr  
               
               
                 #p Leu Thr Thr His Ala  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Leu Phe His Glu Val Ala Lys Leu Asp Val Va  
               
               
                 #l Lys Leu Leu Tyr Asn  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Glu Gln Phe Ala Val Gln Gly Leu Leu Arg Ty  
               
               
                 #r His Thr Tyr Ala Arg  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Phe Gly Ile Glu Ile Gln Val Gln Ile Asn Pr  
               
               
                 #o Thr Pro Phe Gln Gln  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Gly Gly Leu Ile Cys Ala Met Val Pro Gly As  
               
               
                 #p Gln Ser Tyr Gly Ser  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Ile Ala Ser Leu Thr Val Tyr Pro His Gly Le  
               
               
                 #u Leu Asn Cys Asn Ile  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Asn Asn Val Val Arg Ile Lys Val Pro Phe Il  
               
               
                 #e Tyr Thr Arg Gly Ala  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Tyr His Phe Lys Asp Pro Gln Tyr Pro Val Tr  
               
               
                 #p Glu Leu Thr Ile Arg  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Val Trp Ser Glu Leu Asn Ile Gly Thr Gly Th  
               
               
                 #r Ser Ala Tyr Thr Ser  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Leu Asn Val Leu Ala Arg Phe Thr Asp Leu Gl  
               
               
                 #u Leu His Gly Leu Thr  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Pro Leu Ser Thr Gln Met Met Arg Asn Glu Ph  
               
               
                 #e Arg Val Ser Thr Thr  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Glu Asn Val Val Asn Leu Ser Asn Tyr Glu As  
               
               
                 #p Ala Arg Ala Lys Met  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Ser Phe Ala Leu Asp Gln Glu Asp Trp Lys Se  
               
               
                 #r Asp Pro Ser Gln Gly  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Gly Gly Ile Lys Ile Thr His Phe Thr Thr Tr  
               
               
                 #p Thr Ser Ile Pro Thr  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Leu Ala Ala Gln Phe Pro Phe Asn Ala Ser As  
               
               
                 #p Ser Val Gly Gln Gln  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Ile Lys Val Ile Pro Val Asp Pro Tyr Phe Ph  
               
               
                 #e Gln Met Thr Asn Thr  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Asn Pro Asp Gln Lys Cys Ile Thr Ala Leu Al  
               
               
                 #a Ser Ile Cys Gln Met  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Phe Cys Phe Trp Arg Gly Asp Leu Val Phe As  
               
               
                 #p Phe Gln Val Phe Pro  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Thr Lys Tyr His Ser Gly Arg Leu Leu Phe Cy  
               
               
                 #s Phe Val Pro Gly Asn  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Glu Leu Ile Asp Val Thr Gly Ile Thr Leu Ly  
               
               
                 #s Gln Ala Thr Thr Ala  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Pro Cys Ala Val Met Asp Ile Thr Gly Val Gl  
               
               
                 #n Ser Thr Leu Arg Phe  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Arg Val Pro Trp Ile Ser Asp Thr Pro Tyr Ar  
               
               
                 #g Val Asn Arg Tyr Thr  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Lys Ser Ala His Gln Lys Gly Glu Tyr Thr Al  
               
               
                 #a Ile Gly Lys Leu Ile  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Val Tyr Cys Tyr Asn Arg Leu Thr Ser Pro Se  
               
               
                 #r Asn Val Ala Ser His  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Val Arg Val Asn Val Tyr Leu Ser Ala Ile As  
               
               
                 #n Leu Glu Cys Phe Ala  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Pro Leu Tyr His Ala Met Asp Val Thr Thr Gl  
               
               
                 #n Val Gly Asp Asp Ser  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Gly Gly Phe Ser Thr Thr Val Ser Thr Glu Gl  
               
               
                 #n Asn Val Pro Asp Pro  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Gln Val Gly Ile Thr Thr Met Arg Asp Leu Ly  
               
               
                 #s Gly Lys Ala Asn Arg  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Gly Lys Met Asp Val Ser Gly Val Gln Ala Pr  
               
               
                 #o Arg Gly Ser Tyr Gln  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Gln Gln Leu Asn Asp Pro Val Leu Ala Lys Ly  
               
               
                 #s Val Pro Glu Thr Phe  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Pro Glu Leu Lys Pro Gly Glu Ser Arg His Th  
               
               
                 #r Ser Asp His Met Ser  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Ile Tyr Lys Phe Met Gly Arg Ser His Phe Le  
               
               
                 #u Cys Thr Phe Thr Phe  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Asn Ser Asn Asn Lys Glu Tyr Thr Phe Pro Il  
               
               
                 #e Thr Leu Ser Ser Thr  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Ser Asn Pro Pro His Gly Leu Pro Ser Thr Le  
               
               
                 #u Arg Trp Phe Phe Asn  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Leu Phe Gln Leu Tyr Arg Gly Pro Leu Asp Le  
               
               
                 #u Thr Ile Ile Ile Thr  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Gly Ala Thr Asp Val Asp Gly Met Ala Trp Ph  
               
               
                 #e Thr Pro Val Gly Leu  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Ala Val Asp Pro Trp Val Glu Lys Glu Ser Al  
               
               
                 #a Leu Ser Ile Asp Tyr  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Lys Thr Ala Leu Gly Ala Val Arg Phe Asn Th  
               
               
                 #r Arg Arg Thr Gly Asn  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Ile Gln Ile Arg Leu Pro Trp Tyr Ser Tyr Le  
               
               
                 #u Tyr Ala Val Ser Gly  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Ala Leu Asp Gly Leu Gly Asp Lys Thr Asp Se  
               
               
                 #r Thr Phe Gly Leu Phe  
               
               
                 705                 7  
               
               
                 #10                 7  
               
               
                 #15                 7  
               
               
                 #20  
               
               
                   
               
               
                 Leu Phe Glu Ile Ala Asn Tyr Asn His Ser As  
               
               
                 #p Glu Tyr Leu Ser Phe  
               
               
                                 725   
               
               
                 #               730   
               
               
                 #               735  
               
               
                   
               
               
                 Ser Cys Tyr Leu Ser Val Thr Glu Gln Ser Gl  
               
               
                 #u Phe Tyr Phe Pro Arg  
               
               
                             740       
               
               
                 #           745       
               
               
                 #           750  
               
               
                   
               
               
                 Ala Pro Leu Asn Ser Asn Ala Met Leu Ser Th  
               
               
                 #r Glu Ser Met Met Ser  
               
               
                         755           
               
               
                 #       760           
               
               
                 #       765  
               
               
                   
               
               
                 Arg Ile Ala Ala Gly Asp Leu Glu Ser Ser Va  
               
               
                 #l Asp Asp Pro Arg Ser  
               
               
                     770               
               
               
                 #   775               
               
               
                 #   780  
               
               
                   
               
               
                 Glu Glu Asp Arg Arg Phe Glu Ser His Ile Gl  
               
               
                 #u Cys Arg Lys Pro Tyr  
               
               
                 785                 7  
               
               
                 #90                 7  
               
               
                 #95                 8  
               
               
                 #00  
               
               
                   
               
               
                 Lys Glu Leu Arg Leu Glu Val Gly Lys Gln Ar  
               
               
                 #g Leu Lys Tyr Ala Gln  
               
               
                                 805   
               
               
                 #               810   
               
               
                 #               815  
               
               
                   
               
               
                 Glu Glu Leu Ser Asn Glu Val Leu Pro Pro Pr  
               
               
                 #o Arg Lys Met Lys Gly  
               
               
                             820       
               
               
                 #           825       
               
               
                 #           830  
               
               
                   
               
               
                 Leu Phe Ser Gln Ala Lys Ile Ser Leu Phe Ty  
               
               
                 #r Thr Glu Glu His Glu  
               
               
                         835           
               
               
                 #       840           
               
               
                 #       845  
               
               
                   
               
               
                 Ile Met Lys Phe Ser Trp Arg Gly Val Thr Al  
               
               
                 #a Asp Thr Arg Ala Leu  
               
               
                     850               
               
               
                 #   855               
               
               
                 #   860  
               
               
                   
               
               
                 Arg Arg Phe Gly Phe Ser Leu Ala Ala Gly Ar  
               
               
                 #g Ser Val Trp Thr Leu  
               
               
                 865                 8  
               
               
                 #70                 8  
               
               
                 #75                 8  
               
               
                 #80  
               
               
                   
               
               
                 Glu Met Asp Ala Gly Val Leu Thr Gly Arg Le  
               
               
                 #u Ile Arg Leu Asn Asp  
               
               
                                 885   
               
               
                 #               890   
               
               
                 #               895  
               
               
                   
               
               
                 Glu Lys Trp Thr Glu Met Lys Asp Asp Lys Il  
               
               
                 #e Val Ser Leu Ile Glu  
               
               
                             900       
               
               
                 #           905       
               
               
                 #           910  
               
               
                   
               
               
                 Lys Phe Thr Ser Asn Lys Tyr Trp Ser Lys Va  
               
               
                 #l Asn Phe Pro His Gly  
               
               
                         915           
               
               
                 #       920           
               
               
                 #       925  
               
               
                   
               
               
                 Met Leu Asp Leu Glu Glu Ile Ala Ala Asn Se  
               
               
                 #r Lys Asp Phe Pro Asn  
               
               
                     930               
               
               
                 #   935               
               
               
                 #   940  
               
               
                   
               
               
                 Met Ser Glu Thr Asp Leu Cys Phe Leu Leu Hi  
               
               
                 #s Trp Leu Asn Pro Lys  
               
               
                 945                 9  
               
               
                 #50                 9  
               
               
                 #55                 9  
               
               
                 #60  
               
               
                   
               
               
                 Lys Ile Asn Leu Ala Asp Arg Met Leu Gly Le  
               
               
                 #u Ser Gly Val Gln Glu  
               
               
                                 965   
               
               
                 #               970   
               
               
                 #               975  
               
               
                   
               
               
                 Ile Lys Glu Gln Gly Val Gly Leu Ile Ala Gl  
               
               
                 #u Cys Arg Thr Phe Leu  
               
               
                             980       
               
               
                 #           985       
               
               
                 #           990  
               
               
                   
               
               
                 Asp Ser Ile Ala Gly Thr Leu Lys Ser Met Me  
               
               
                 #t Phe Gly Phe His His  
               
               
                         995           
               
               
                 #       1000           
               
               
                 #      1005  
               
               
                   
               
               
                 Ser Val Thr Val Glu Ile Ile Asn Thr Val Le  
               
               
                 #u Cys Phe Val Lys Ser  
               
               
                     1010              
               
               
                 #   1015               
               
               
                 #  1020  
               
               
                   
               
               
                 Gly Ile Leu Leu Tyr Val Ile Gln Gln Leu As  
               
               
                 #n Gln Asp Glu His Ser  
               
               
                 1025                1030  
               
               
                 #                1035  
               
               
                 #               1040  
               
               
                   
               
               
                 His Ile Ile Gly Leu Leu Arg Val Met Asn Ty  
               
               
                 #r Ala Asp Ile Gly Cys  
               
               
                                 1045  
               
               
                 #               1050   
               
               
                 #              1055  
               
               
                   
               
               
                 Ser Val Ile Ser Cys Gly Lys Val Phe Ser Ly  
               
               
                 #s Met Leu Glu Thr Val  
               
               
                             1060      
               
               
                 #           1065       
               
               
                 #          1070  
               
               
                   
               
               
                 Phe Asn Trp Gln Met Asp Ser Arg Met Met Gl  
               
               
                 #u Leu Arg Thr Gln Ser  
               
               
                         1075          
               
               
                 #       1080           
               
               
                 #      1085  
               
               
                   
               
               
                 Phe Ser Asn Trp Leu Arg Asp Ile Cys Ser Gl  
               
               
                 #y Ile Thr Ile Phe Lys  
               
               
                     1090              
               
               
                 #   1095               
               
               
                 #  1100  
               
               
                   
               
               
                 Ser Phe Lys Asp Ala Ile Tyr Trp Leu Tyr Th  
               
               
                 #r Lys Leu Lys Asp Phe  
               
               
                 1105                1110  
               
               
                 #                1115  
               
               
                 #               1120  
               
               
                   
               
               
                 Tyr Glu Val Asn Tyr Gly Lys Lys Lys Asp Il  
               
               
                 #e Leu Asn Ile Leu Lys  
               
               
                                 1125  
               
               
                 #               1130   
               
               
                 #              1135  
               
               
                   
               
               
                 Asp Asn Gln Gln Lys Ile Glu Lys Ala Ile Gl  
               
               
                 #u Glu Ala Asp Asn Phe  
               
               
                             1140      
               
               
                 #           1145       
               
               
                 #          1150  
               
               
                   
               
               
                 Cys Ile Leu Gln Ile Gln Asp Val Glu Lys Ph  
               
               
                 #e Asp Gln Tyr Gln Lys  
               
               
                         1155          
               
               
                 #       1160           
               
               
                 #      1165  
               
               
                   
               
               
                 Gly Val Asp Leu Ile Gln Lys Leu Arg Thr Va  
               
               
                 #l His Ser Met Ala Gln  
               
               
                     1170              
               
               
                 #   1175               
               
               
                 #  1180  
               
               
                   
               
               
                 Val Asp Pro Asn Leu Gly Val His Leu Ser Pr  
               
               
                 #o Leu Arg Asp Cys Ile  
               
               
                 1185                1190  
               
               
                 #                1195  
               
               
                 #               1200  
               
               
                   
               
               
                 Ala Arg Val His Gln Lys Leu Lys Asn Leu Gl  
               
               
                 #y Ser Ile Asn Gln Ala  
               
               
                                 1205  
               
               
                 #               1210   
               
               
                 #              1215  
               
               
                   
               
               
                 Met Val Thr Arg Cys Glu Pro Val Val Cys Ty  
               
               
                 #r Leu Tyr Gly Lys Arg  
               
               
                             1220      
               
               
                 #           1225       
               
               
                 #          1230  
               
               
                   
               
               
                 Gly Gly Gly Lys Ser Leu Thr Ser Ile Ala Le  
               
               
                 #u Ala Thr Lys Ile Cys  
               
               
                         1235          
               
               
                 #       1240           
               
               
                 #      1245  
               
               
                   
               
               
                 Lys His Tyr Gly Val Glu Pro Glu Lys Asn Il  
               
               
                 #e Tyr Thr Lys Pro Val  
               
               
                     1250              
               
               
                 #   1255               
               
               
                 #  1260  
               
               
                   
               
               
                 Ala Ser Asp Tyr Trp Asp Gly Tyr Ser Gly Gl  
               
               
                 #n Leu Val Cys Ile Ile  
               
               
                 1265                1270  
               
               
                 #                1275  
               
               
                 #               1280  
               
               
                   
               
               
                 Asp Asp Ile Gly Gln Asn Thr Thr Asp Glu As  
               
               
                 #p Trp Ser Asp Phe Cys  
               
               
                                 1285  
               
               
                 #               1290   
               
               
                 #              1295  
               
               
                   
               
               
                 Gln Leu Val Ser Gly Cys Pro Met Arg Leu As  
               
               
                 #n Met Ala Ser Leu Glu  
               
               
                             1300      
               
               
                 #           1305       
               
               
                 #          1310  
               
               
                   
               
               
                 Glu Lys Gly Arg His Phe Ser Ser Pro Phe Il  
               
               
                 #e Ile Ala Thr Ser Asn  
               
               
                         1315          
               
               
                 #       1320           
               
               
                 #      1325  
               
               
                   
               
               
                 Trp Ser Asn Pro Ser Pro Lys Thr Val Tyr Va  
               
               
                 #l Lys Glu Ala Ile Asp  
               
               
                     1330              
               
               
                 #   1335               
               
               
                 #  1340  
               
               
                   
               
               
                 Arg Arg Leu His Phe Lys Val Glu Val Lys Pr  
               
               
                 #o Ala Ser Phe Phe Lys  
               
               
                 1345                1350  
               
               
                 #                1355  
               
               
                 #               1360  
               
               
                   
               
               
                 Asn Pro His Asn Asp Met Leu Asn Val Asn Le  
               
               
                 #u Ala Lys Thr Asn Asp  
               
               
                                 1365  
               
               
                 #               1370   
               
               
                 #              1375  
               
               
                   
               
               
                 Ala Ile Lys Asp Met Ser Cys Val Asp Leu Il  
               
               
                 #e Met Asp Gly His Asn  
               
               
                             1380      
               
               
                 #           1385       
               
               
                 #          1390  
               
               
                   
               
               
                 Ile Ser Leu Met Asp Leu Leu Ser Ser Leu Va  
               
               
                 #l Met Thr Val Glu Ile  
               
               
                         1395          
               
               
                 #       1400           
               
               
                 #      1405  
               
               
                   
               
               
                 Arg Lys Gln Asn Met Ser Glu Phe Met Glu Le  
               
               
                 #u Trp Ser Gln Gly Ile  
               
               
                     1410              
               
               
                 #   1415               
               
               
                 #  1420  
               
               
                   
               
               
                 Ser Asp Asp Asp Asn Asp Ser Ala Val Ala Gl  
               
               
                 #u Phe Phe Gln Ser Phe  
               
               
                 1425                1430  
               
               
                 #                1435  
               
               
                 #               1440  
               
               
                   
               
               
                 Pro Ser Gly Glu Pro Ser Asn Trp Lys Leu Se  
               
               
                 #r Ser Phe Phe Gln Ser  
               
               
                                 1445  
               
               
                 #               1450   
               
               
                 #              1455  
               
               
                   
               
               
                 Val Thr Asn His Lys Trp Val Ala Val Gly Al  
               
               
                 #a Ala Val Gly Ile Leu  
               
               
                             1460      
               
               
                 #           1465       
               
               
                 #          1470  
               
               
                   
               
               
                 Gly Val Leu Val Gly Gly Trp Phe Val Tyr Ly  
               
               
                 #s His Phe Ser Arg Lys  
               
               
                         1475          
               
               
                 #       1480           
               
               
                 #      1485  
               
               
                   
               
               
                 Glu Glu Glu Pro Ile Pro Ala Glu Gly Val Ty  
               
               
                 #r His Gly Val Thr Lys  
               
               
                     1490              
               
               
                 #   1495               
               
               
                 #  1500  
               
               
                   
               
               
                 Pro Lys Gln Val Ile Lys Leu Asp Ala Asp Pr  
               
               
                 #o Val Glu Ser Gln Ser  
               
               
                 1505                1510  
               
               
                 #                1515  
               
               
                 #               1520  
               
               
                   
               
               
                 Thr Leu Glu Ile Ala Gly Leu Val Arg Lys As  
               
               
                 #n Leu Val Gln Phe Gly  
               
               
                                 1525  
               
               
                 #               1530   
               
               
                 #              1535  
               
               
                   
               
               
                 Val Gly Glu Lys Asn Gly Cys Val Arg Trp Va  
               
               
                 #l Met Asn Ala Leu Gly  
               
               
                             1540      
               
               
                 #           1545       
               
               
                 #          1550  
               
               
                   
               
               
                 Val Lys Asp Asp Trp Leu Leu Val Pro Ser Hi  
               
               
                 #s Ala Tyr Lys Phe Glu  
               
               
                         1555          
               
               
                 #       1560           
               
               
                 #      1565  
               
               
                   
               
               
                 Lys Asp Tyr Glu Met Met Glu Phe Tyr Phe As  
               
               
                 #n Arg Gly Gly Thr Tyr  
               
               
                     1570              
               
               
                 #   1575               
               
               
                 #  1580  
               
               
                   
               
               
                 Tyr Ser Ile Ser Ala Gly Asn Val Val Ile Gl  
               
               
                 #n Ser Leu Asp Val Gly  
               
               
                 1585                1590  
               
               
                 #                1595  
               
               
                 #               1600  
               
               
                   
               
               
                 Phe Gln Asp Val Val Leu Met Lys Val Pro Th  
               
               
                 #r Ile Pro Lys Phe Arg  
               
               
                                 1605  
               
               
                 #               1610   
               
               
                 #              1615  
               
               
                   
               
               
                 Asp Ile Thr Gln His Phe Ile Lys Lys Gly As  
               
               
                 #p Val Pro Arg Ala Leu  
               
               
                             1620      
               
               
                 #           1625       
               
               
                 #          1630  
               
               
                   
               
               
                 Asn Arg Leu Ala Thr Leu Val Thr Thr Val As  
               
               
                 #n Gly Thr Pro Met Leu  
               
               
                         1635          
               
               
                 #       1640           
               
               
                 #      1645  
               
               
                   
               
               
                 Ile Ser Glu Gly Pro Leu Lys Met Glu Glu Ly  
               
               
                 #s Ala Thr Tyr Val His  
               
               
                     1650              
               
               
                 #   1655               
               
               
                 #  1660  
               
               
                   
               
               
                 Lys Lys Asn Asp Gly Thr Thr Val Asp Leu Th  
               
               
                 #r Val Asp Gln Ala Trp  
               
               
                 1665                1670  
               
               
                 #                1675  
               
               
                 #               1680  
               
               
                   
               
               
                 Arg Gly Lys Gly Glu Gly Leu Pro Gly Met Cy  
               
               
                 #s Gly Gly Ala Leu Val  
               
               
                                 1685  
               
               
                 #               1690   
               
               
                 #              1695  
               
               
                   
               
               
                 Ser Ser Asn Gln Ser Ile Gln Asn Ala Ile Le  
               
               
                 #u Gly Ile His Val Ala  
               
               
                             1700      
               
               
                 #           1705       
               
               
                 #          1710  
               
               
                   
               
               
                 Gly Gly Asn Ser Ile Leu Val Ala Lys Leu Va  
               
               
                 #l Thr Gln Glu Met Phe  
               
               
                         1715          
               
               
                 #       1720           
               
               
                 #      1725  
               
               
                   
               
               
                 Gln Asn Ile Asp Lys Lys Ile Glu Ser Gln Ar  
               
               
                 #g Ile Met Lys Val Glu  
               
               
                     1730              
               
               
                 #   1735               
               
               
                 #  1740  
               
               
                   
               
               
                 Phe Thr Gln Cys Ser Met Asn Val Val Ser Ly  
               
               
                 #s Thr Leu Phe Arg Lys  
               
               
                 1745                1750  
               
               
                 #                1755  
               
               
                 #               1760  
               
               
                   
               
               
                 Ser Pro Ile His His His Ile Asp Lys Thr Me  
               
               
                 #t Ile Asn Phe Pro Ala  
               
               
                                 1765  
               
               
                 #               1770   
               
               
                 #              1775  
               
               
                   
               
               
                 Ala Met Pro Phe Ser Lys Ala Glu Ile Asp Pr  
               
               
                 #o Met Ala Met Met Leu  
               
               
                             1780      
               
               
                 #           1785       
               
               
                 #          1790  
               
               
                   
               
               
                 Ser Lys Tyr Ser Leu Pro Ile Val Glu Glu Pr  
               
               
                 #o Glu Asp Tyr Lys Glu  
               
               
                         1795          
               
               
                 #       1800           
               
               
                 #      1805  
               
               
                   
               
               
                 Ala Ser Val Phe Tyr Gln Asn Lys Ile Val Gl  
               
               
                 #y Lys Thr Gln Leu Val  
               
               
                     1810              
               
               
                 #   1815               
               
               
                 #  1820  
               
               
                   
               
               
                 Asp Asp Phe Leu Asp Leu Asp Met Ala Ile Th  
               
               
                 #r Gly Ala Pro Gly Ile  
               
               
                 1825                1830  
               
               
                 #                1835  
               
               
                 #               1840  
               
               
                   
               
               
                 Asp Ala Ile Asn Met Asp Ser Ser Pro Gly Ph  
               
               
                 #e Pro Tyr Val Gln Glu  
               
               
                                 1845  
               
               
                 #               1850   
               
               
                 #              1855  
               
               
                   
               
               
                 Lys Leu Thr Lys Arg Asp Leu Ile Trp Leu As  
               
               
                 #p Glu Asn Gly Leu Leu  
               
               
                             1860      
               
               
                 #           1865       
               
               
                 #          1870  
               
               
                   
               
               
                 Leu Gly Val His Pro Arg Leu Ala Gln Arg Il  
               
               
                 #e Leu Phe Asn Thr Val  
               
               
                         1875          
               
               
                 #       1880           
               
               
                 #      1885  
               
               
                   
               
               
                 Met Met Glu Asn Cys Ser Asp Leu Asp Val Va  
               
               
                 #l Phe Thr Thr Cys Pro  
               
               
                     1890              
               
               
                 #   1895               
               
               
                 #  1900  
               
               
                   
               
               
                 Lys Asp Glu Leu Arg Pro Leu Glu Lys Val Le  
               
               
                 #u Glu Ser Lys Thr Arg  
               
               
                 1905                1910  
               
               
                 #                1915  
               
               
                 #               1920  
               
               
                   
               
               
                 Ala Ile Asp Ala Cys Pro Leu Asp Tyr Thr Il  
               
               
                 #e Leu Cys Arg Met Tyr  
               
               
                                 1925  
               
               
                 #               1930   
               
               
                 #              1935  
               
               
                   
               
               
                 Trp Gly Pro Ala Ile Ser Tyr Phe His Leu As  
               
               
                 #n Pro Gly Phe His Thr  
               
               
                             1940      
               
               
                 #           1945       
               
               
                 #          1950  
               
               
                   
               
               
                 Gly Val Ala Ile Gly Ile Asp Pro Asp Arg Gl  
               
               
                 #n Trp Asp Glu Leu Phe  
               
               
                         1955          
               
               
                 #       1960           
               
               
                 #      1965  
               
               
                   
               
               
                 Lys Thr Met Ile Arg Phe Gly Asp Val Gly Le  
               
               
                 #u Asp Leu Asp Phe Ser  
               
               
                     1970              
               
               
                 #   1975               
               
               
                 #  1980  
               
               
                   
               
               
                 Ala Phe Asp Ala Ser Leu Ser Pro Phe Met Il  
               
               
                 #e Arg Glu Ala Gly Arg  
               
               
                 1985                1990  
               
               
                 #                1995  
               
               
                 #               2000  
               
               
                   
               
               
                 Ile Met Ser Glu Leu Ser Gly Thr Pro Ser Hi  
               
               
                 #s Phe Gly Thr Ala Leu  
               
               
                                 2005  
               
               
                 #               2010   
               
               
                 #              2015  
               
               
                   
               
               
                 Ile Asn Thr Ile Ile Tyr Ser Lys His Leu Le  
               
               
                 #u Tyr Asn Cys Cys Tyr  
               
               
                             2020      
               
               
                 #           2025       
               
               
                 #          2030  
               
               
                   
               
               
                 His Val Cys Gly Ser Met Pro Ser Gly Ser Pr  
               
               
                 #o Cys Thr Ala Leu Leu  
               
               
                         2035          
               
               
                 #       2040           
               
               
                 #      2045  
               
               
                   
               
               
                 Asn Ser Ile Ile Asn Asn Ile Asn Leu Tyr Ty  
               
               
                 #r Val Phe Ser Lys Ile  
               
               
                     2050              
               
               
                 #   2055               
               
               
                 #  2060  
               
               
                   
               
               
                 Phe Gly Lys Ser Pro Val Phe Phe Cys Gln Al  
               
               
                 #a Leu Arg Ile Leu Cys  
               
               
                 2065                2070  
               
               
                 #                2075  
               
               
                 #               2080  
               
               
                   
               
               
                 Tyr Gly Asp Asp Val Leu Ile Val Phe Ser Ar  
               
               
                 #g Asp Val Gln Ile Asp  
               
               
                                 2085  
               
               
                 #               2090   
               
               
                 #              2095  
               
               
                   
               
               
                 Asn Leu Asp Leu Ile Gly Gln Lys Ile Val As  
               
               
                 #p Glu Phe Lys Lys Leu  
               
               
                             2100      
               
               
                 #           2105       
               
               
                 #          2110  
               
               
                   
               
               
                 Gly Met Thr Ala Thr Ser Ala Asp Lys Asn Va  
               
               
                 #l Pro Gln Leu Lys Pro  
               
               
                         2115          
               
               
                 #       2120           
               
               
                 #      2125  
               
               
                   
               
               
                 Val Ser Glu Leu Thr Phe Leu Lys Arg Ser Ph  
               
               
                 #e Asn Leu Val Glu Asp  
               
               
                     2130              
               
               
                 #   2135               
               
               
                 #  2140  
               
               
                   
               
               
                 Arg Ile Arg Pro Ala Ile Ser Glu Lys Thr Il  
               
               
                 #e Trp Ser Leu Met Ala  
               
               
                 2145                2150  
               
               
                 #                2155  
               
               
                 #               2160  
               
               
                   
               
               
                 Trp Gln Arg Ser Asn Ala Glu Phe Glu Gln As  
               
               
                 #n Leu Glu Asn Ala Gln  
               
               
                                 2165  
               
               
                 #               2170   
               
               
                 #              2175  
               
               
                   
               
               
                 Trp Phe Ala Phe Met His Gly Tyr Glu Phe Ty  
               
               
                 #r Gln Lys Phe Tyr Tyr  
               
               
                             2180      
               
               
                 #           2185       
               
               
                 #          2190  
               
               
                   
               
               
                 Phe Val Gln Ser Cys Leu Glu Lys Glu Met Il  
               
               
                 #e Glu Tyr Arg Leu Lys  
               
               
                         2195          
               
               
                 #       2200           
               
               
                 #      2205  
               
               
                   
               
               
                 Ser Tyr Asp Trp Trp Arg Met Arg Phe Tyr As  
               
               
                 #p Gln Cys Phe Ile Cys  
               
               
                     2210              
               
               
                 #   2215               
               
               
                 #  2220  
               
               
                   
               
               
                 Asp Leu Ser  
               
               
                 2225  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 13  
               
               
                 &lt;211&gt; LENGTH: 9416  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis C virus sequenc  
               
               
                 #e  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 13  
               
               
                   
               
               
                 gccagccccc tgatgggggc gacactccac catgaatcac tcccctgtga gg  
               
               
                 #aactactg     60  
               
               
                   
               
               
                 tcttcacgca gaaagcgtct agccatggcg ttagtatgag tgtcgtgcag cc  
               
               
                 #tccaggac    120  
               
               
                   
               
               
                 cccccctccc gggagagcca tagtggtctg cggaaccggt gagtacaccg ga  
               
               
                 #attgccag    180  
               
               
                   
               
               
                 gacgaccggg tcctttcttg gataaacccg ctcaatgcct ggagatttgg gc  
               
               
                 #gtgccccc    240  
               
               
                   
               
               
                 gcaagactgc tagccgagta gtgttgggtc gcgaaaggcc ttgtggtact gc  
               
               
                 #ctgatagg    300  
               
               
                   
               
               
                 gtgcttgcga gtgccccggg aggtctcgta gaccgtgcac catgagcacg aa  
               
               
                 #tcctaaac    360  
               
               
                   
               
               
                 ctcaaagaaa aaccaaacgt aacaccaacc gtcgcccaca ggacgtcaag tt  
               
               
                 #cccgggtg    420  
               
               
                   
               
               
                 gcggtcagat cgttggtgga gtttacttgt tgccgcgcag gggccctaga tt  
               
               
                 #gggtgtgc    480  
               
               
                   
               
               
                 gcgcgacgag gaagacttcc gagcggtcgc aacctcgagg tagacgtcag cc  
               
               
                 #tatcccca    540  
               
               
                   
               
               
                 aggcacgtcg gcccgagggc aggacctggg ctcagcccgg gtacccttgg cc  
               
               
                 #cctctatg    600  
               
               
                   
               
               
                 gcaatgaggg ttgcgggtgg gcgggatggc tcctgtctcc ccgtggctct cg  
               
               
                 #gcctagct    660  
               
               
                   
               
               
                 ggggccccac agacccccgg cgtaggtcgc gcaatttggg taaggtcatc ga  
               
               
                 #taccctta    720  
               
               
                   
               
               
                 cgtgcggctt cgccgacctc atggggtaca taccgctcgt cggcgcccct ct  
               
               
                 #tggaggcg    780  
               
               
                   
               
               
                 ctgccagggc cctggcgcat ggcgtccggg ttctggaaga cggcgtgaac ta  
               
               
                 #tgcaacag    840  
               
               
                   
               
               
                 ggaaccttcc tggttgctct ttctctatct tccttctggc cctgctctct tg  
               
               
                 #cctgactg    900  
               
               
                   
               
               
                 tgcccgcttc agcctaccaa gtgcgcaatt cctcggggct ttaccatgtc ac  
               
               
                 #caatgatt    960  
               
               
                   
               
               
                 gccctaactc gagtgttgtg tacgaggcgg ccgatgccat cctgcacact cc  
               
               
                 #ggggtgtg   1020  
               
               
                   
               
               
                 tcccttgcgt tcgcgagggt aacgcctcga ggtgttgggt ggcggtgacc cc  
               
               
                 #cacggtgg   1080  
               
               
                   
               
               
                 ccaccaggga cggcaaactc cccacaacgc agcttcgacg tcatatcgat ct  
               
               
                 #gcttgtcg   1140  
               
               
                   
               
               
                 ggagcgccac cctctgctcg gccctctacg tgggggacct gtgcgggtct gt  
               
               
                 #ctttcttg   1200  
               
               
                   
               
               
                 ttggtcaact gtttaccttc tctcccaggc accactggac gacgcaagac tg  
               
               
                 #caattgtt   1260  
               
               
                   
               
               
                 ctatctatcc cggccatata acgggtcatc gcatggcatg gaatatgatg at  
               
               
                 #gaactggt   1320  
               
               
                   
               
               
                 cccctacggc agcgttggtg gtagctcagc tgctccgaat cccacaagcc at  
               
               
                 #catggaca   1380  
               
               
                   
               
               
                 tgatcgctgg cgcccactgg ggagtcctgg cgggcataaa gtatttctcc at  
               
               
                 #ggtgggga   1440  
               
               
                   
               
               
                 actgggcgaa ggtcctggta gtgctgctgc tatttgccgg cgtcgacgcg ga  
               
               
                 #aacccacg   1500  
               
               
                   
               
               
                 tcaccggggg aaatgccggc cgcaccacgg ctgggcttgt tggtctcctt ac  
               
               
                 #accaggcg   1560  
               
               
                   
               
               
                 ccaagcagaa catccaactg atcaacacca acggcagttg gcacatcaat ag  
               
               
                 #cacggcct   1620  
               
               
                   
               
               
                 tgaactgcaa tgaaagcctt aacaccggct ggttagcagg gctcttctat ca  
               
               
                 #gcacaaat   1680  
               
               
                   
               
               
                 tcaactcttc aggctgtcct gagaggttgg ccagctgccg acgccttacc ga  
               
               
                 #ttttgccc   1740  
               
               
                   
               
               
                 agggctgggg tcctatcagt tatgccaacg gaagcggcct cgacgaacgc cc  
               
               
                 #ctactgct   1800  
               
               
                   
               
               
                 ggcactaccc tccaagacct tgtggcattg tgcccgcaaa gagcgtgtgt gg  
               
               
                 #cccggtat   1860  
               
               
                   
               
               
                 attgcttcac tcccagcccc gtggtggtgg gaacgaccga caggtcgggc gc  
               
               
                 #gcctacct   1920  
               
               
                   
               
               
                 acagctgggg tgcaaatgat acggatgtct tcgtccttaa caacaccagg cc  
               
               
                 #accgctgg   1980  
               
               
                   
               
               
                 gcaattggtt cggttgtacc tggatgaact caactggatt caccaaagtg tg  
               
               
                 #cggagcgc   2040  
               
               
                   
               
               
                 ccccttgtgt catcggaggg gtgggcaaca acaccttgct ctgccccact ga  
               
               
                 #ttgcttcc   2100  
               
               
                   
               
               
                 gcaaatatcc ggaagccaca tactctcggt gcggctccgg tcccaggatt ac  
               
               
                 #acccaggt   2160  
               
               
                   
               
               
                 gcatggtcga ctacccgtat aggctttggc actatccttg taccatcaat ta  
               
               
                 #caccatat   2220  
               
               
                   
               
               
                 tcaaagtcag gatgtacgtg ggaggggtcg agcacaggct ggaagcggcc tg  
               
               
                 #caactgga   2280  
               
               
                   
               
               
                 cgcggggcga acgctgtgat ctggaagaca gggacaggtc cgagctcagc cc  
               
               
                 #gttgctgc   2340  
               
               
                   
               
               
                 tgtccaccac acagtggcag gtccttccgt gttctttcac gaccctgcca gc  
               
               
                 #cttgtcca   2400  
               
               
                   
               
               
                 ccggcctcat ccacctccac cagaacattg tggacgtgca gtacttgtac gg  
               
               
                 #ggtagggt   2460  
               
               
                   
               
               
                 caagcatcgc gtcctgggcc attaagtggg agtacgtcgt tctcctgttc ct  
               
               
                 #tctgcttg   2520  
               
               
                   
               
               
                 cagacgcgcg cgtctgttcc tgcttgtgga tgatgttact catatcccaa gc  
               
               
                 #ggaggcgg   2580  
               
               
                   
               
               
                 ctttggagaa cctcgtaata ctcaatgcag catccctggc cgggacgcat gg  
               
               
                 #tcttgtgt   2640  
               
               
                   
               
               
                 ccttcctcgt gttcttctgc tttgcgtggt atctgaaggg taggtgggtg cc  
               
               
                 #cggagcgg   2700  
               
               
                   
               
               
                 tctacgccct ctacgggatg tggcctctcc tcctgctcct gctggcgttg cc  
               
               
                 #tcagcggg   2760  
               
               
                   
               
               
                 catacgcact ggacacggag gtggccgcgt cgtgtggcgg cgttgttctt gt  
               
               
                 #cgggttaa   2820  
               
               
                   
               
               
                 tggcgctgac tctgtcgcca tattacaagc gctatatcag ctggtgcatg tg  
               
               
                 #gtggcttc   2880  
               
               
                   
               
               
                 agtattttct gaccagagta gaagcgcaac tgcacgtgtg ggttcccccc ct  
               
               
                 #caacgtcc   2940  
               
               
                   
               
               
                 ggggggggcg cgatgccgtc atcttactca cgtgtgtagt acacccggcc ct  
               
               
                 #ggtatttg   3000  
               
               
                   
               
               
                 acatcaccaa actactcctg gccatcttcg gacccctttg gattcttcaa gc  
               
               
                 #cagtttgc   3060  
               
               
                   
               
               
                 ttaaagtccc ctacttcgtg cgcgttcaag gccttctccg gatctgcgcg ct  
               
               
                 #agcgcgga   3120  
               
               
                   
               
               
                 agatagccgg aggtcattac gtgcaaatgg ccatcatcaa gttaggggcg ct  
               
               
                 #tactggca   3180  
               
               
                   
               
               
                 cctgtgtgta taaccatctc gctcctcttc gagactgggc gcacaacggc ct  
               
               
                 #gcgagatc   3240  
               
               
                   
               
               
                 tggccgtggc tgtggaacca gtcgtcttct cccgaatgga gaccaagctc at  
               
               
                 #cacgtggg   3300  
               
               
                   
               
               
                 gggcagatac cgccgcgtgc ggtgacatca tcaacggctt gcccgtctct gc  
               
               
                 #ccgtaggg   3360  
               
               
                   
               
               
                 gccaggagat actgcttggg ccagccgacg gaatggtctc caaggggtgg ag  
               
               
                 #gttgctgg   3420  
               
               
                   
               
               
                 cgcccatcac ggcgtacgcc cagcagacga gaggcctcct agggtgtata at  
               
               
                 #caccagcc   3480  
               
               
                   
               
               
                 tgactggccg ggacaaaaac caagtggagg gtgaggtcca gatcgtgtca ac  
               
               
                 #tgctaccc   3540  
               
               
                   
               
               
                 agaccttcct ggcaacgtgc atcaatgggg tatgctggac tgtctaccac gg  
               
               
                 #ggccggaa   3600  
               
               
                   
               
               
                 cgaggaccat cgcatcaccc aagggtcctg tcatccagac gtataccaat gt  
               
               
                 #ggatcaag   3660  
               
               
                   
               
               
                 acctcgtggg ctggcccgct cctcaaggtt cccgctcatt gacaccctgc ac  
               
               
                 #ctgcggct   3720  
               
               
                   
               
               
                 cctcggacct ttacctggtc acgaggcacg ccgatgtcat tcccgtgcgc cg  
               
               
                 #gcgaggtg   3780  
               
               
                   
               
               
                 atagcagggg tagcctgctt tcgccccggc ccatttccta cttgaaaggc tc  
               
               
                 #ctcggggg   3840  
               
               
                   
               
               
                 gtccgctgtt gtgccccacg ggacacgccg tgggcctatt cagggccgcg gt  
               
               
                 #gtgcaccc   3900  
               
               
                   
               
               
                 gtggagtggc taaggcggtg gactttatcc ctgtggagaa cctagagaca ac  
               
               
                 #catgagat   3960  
               
               
                   
               
               
                 ccccggtgtt cacggacaac tcctctccac cagcagtgcc ccagagcttc ca  
               
               
                 #ggtggccc   4020  
               
               
                   
               
               
                 acctgcatgc tcccaccggc agcggtaaga gcaccaaggt cccggctgcg ta  
               
               
                 #cgcagcca   4080  
               
               
                   
               
               
                 agggctacaa ggtgttggtg ctcaacccct ctgttgctgc aacactgggc tt  
               
               
                 #tggtgctt   4140  
               
               
                   
               
               
                 acatgtccaa ggcccatggg gttgatccta atatcaggac cggggtgaga ac  
               
               
                 #aattacca   4200  
               
               
                   
               
               
                 ctggcagccc catcacgtac tccacctacg gcaagttcct tgccgacgcc gg  
               
               
                 #gtgctcag   4260  
               
               
                   
               
               
                 gaggtgctta tgacataata atttgtgacg agtgccactc cacggatgcc ac  
               
               
                 #atccatct   4320  
               
               
                   
               
               
                 cgggcatcgg cactgtcctt gaccaagcag agactgcggg ggcgagactg gt  
               
               
                 #tgtgctcg   4380  
               
               
                   
               
               
                 ccactgctac ccctccgggc tccgtcactg tgtcccatcc taacatcgag ga  
               
               
                 #ggttgctc   4440  
               
               
                   
               
               
                 tgtccaccac cggagagatc cccttttacg gcaaggctat ccccctcgag gt  
               
               
                 #gatcaagg   4500  
               
               
                   
               
               
                 ggggaagaca tctcatcttc tgccactcaa agaagaagtg cgacgagctc gc  
               
               
                 #cgcgaagc   4560  
               
               
                   
               
               
                 tggtcgcatt gggcatcaat gccgtggcct actaccgcgg tcttgacgtg tc  
               
               
                 #tgtcatcc   4620  
               
               
                   
               
               
                 cgaccagcgg cgatgttgtc gtcgtgtcga ccgatgctct catgactggc tt  
               
               
                 #taccggcg   4680  
               
               
                   
               
               
                 acttcgactc tgtgatagac tgcaacacgt gtgtcactca gacagtcgat tt  
               
               
                 #tagccttg   4740  
               
               
                   
               
               
                 accctacctt taccattgag acaaccacgc tcccccagga tgctgtctcc ag  
               
               
                 #gactcaac   4800  
               
               
                   
               
               
                 gccggggcag gactggcagg gggaagccag gcatctatag atttgtggca cc  
               
               
                 #gggggagc   4860  
               
               
                   
               
               
                 gcccctccgg catgttcgac tcgtccgtcc tctgtgagtg ctatgacgcg gg  
               
               
                 #ctgtgctt   4920  
               
               
                   
               
               
                 ggtatgagct cacgcccgcc gagactacag ttaggctacg agcgtacatg aa  
               
               
                 #caccccgg   4980  
               
               
                   
               
               
                 ggcttcccgt gtgccaggac catcttggat tttgggaggg cgtctttacg gg  
               
               
                 #cctcactc   5040  
               
               
                   
               
               
                 atatagatgc ccactttcta tcccagacaa agcagagtgg ggagaacttt cc  
               
               
                 #ttacctgg   5100  
               
               
                   
               
               
                 tagcgtacca agccaccgtg tgcgctaggg ctcaagcccc tcccccatcg tg  
               
               
                 #ggaccaga   5160  
               
               
                   
               
               
                 tgcggaagtg tttgatccgc cttaaaccca ccctccatgg gccaacaccc ct  
               
               
                 #gctataca   5220  
               
               
                   
               
               
                 gactgggcgc tgttcagaat gaagtcaccc tgacgcaccc aatcaccaaa ta  
               
               
                 #catcatga   5280  
               
               
                   
               
               
                 catgcatgtc ggccgacctg gaggtcgtca cgagcacctg ggtgctcgtt gg  
               
               
                 #cggcgtcc   5340  
               
               
                   
               
               
                 tggctgctct ggccgcgtat tgcctgtcaa caggctgcgt ggtcatagtg gg  
               
               
                 #caggatcg   5400  
               
               
                   
               
               
                 tcttgtccgg gaagccggca attatacctg acagggaggt tctctaccag ga  
               
               
                 #gttcgatg   5460  
               
               
                   
               
               
                 agatggaaga gtgctctcag cacttaccgt acatcgagca agggatgatg ct  
               
               
                 #cgctgagc   5520  
               
               
                   
               
               
                 agttcaagca gaaggccctc ggcctcctgc agaccgcgtc ccgccatgca ga  
               
               
                 #ggttatca   5580  
               
               
                   
               
               
                 cccctgctgt ccagaccaac tggcagaaac tcgaggtctt ttgggcgaag ca  
               
               
                 #catgtgga   5640  
               
               
                   
               
               
                 atttcatcag tgggatacaa tacttggcgg gcctgtcaac gctgcctggt aa  
               
               
                 #ccccgcca   5700  
               
               
                   
               
               
                 ttgcttcatt gatggctttt acagctgccg tcaccagccc actaaccact gg  
               
               
                 #ccaaaccc   5760  
               
               
                   
               
               
                 tcctcttcaa catattgggg gggtgggtgg ctgcccagct cgccgccccc gg  
               
               
                 #tgccgcta   5820  
               
               
                   
               
               
                 ccgcctttgt gggcgctggc ttagctggcg ccgcactcga cagcgttgga ct  
               
               
                 #ggggaagg   5880  
               
               
                   
               
               
                 tcctcgtgga cattcttgca ggctatggcg cgggcgtggc gggagctctt gt  
               
               
                 #ggcattca   5940  
               
               
                   
               
               
                 agatcatgag cggtgaggtc ccctccacgg aggacctggt caatctgctg cc  
               
               
                 #cgccatcc   6000  
               
               
                   
               
               
                 tctcacctgg agcccttgca gtcggtgtgg tctttgcatc aatactgcgc cg  
               
               
                 #gcgtgttg   6060  
               
               
                   
               
               
                 gcccgggcga gggggcagtg caatggatga accggctaat agccttcgcc tc  
               
               
                 #ccggggga   6120  
               
               
                   
               
               
                 accatgtttc ccccacacac tacgtgccgg agagcgatgc agccgcccgc gt  
               
               
                 #cactgcca   6180  
               
               
                   
               
               
                 tactcagcag cctcactgta acccagctcc tgaggcgact gcatcagtgg at  
               
               
                 #aagctcgg   6240  
               
               
                   
               
               
                 agtgtaccac tccatgctcc ggttcctggc taagggacat ctgggactgg at  
               
               
                 #atgcgagg   6300  
               
               
                   
               
               
                 tgctgagcga ctttaagacc tggctgaaag ccaagctcat gccacaactg cc  
               
               
                 #tgggattc   6360  
               
               
                   
               
               
                 cctttgtgtc ctgccagcgc gggtataggg gggtctggcg aggagacggc at  
               
               
                 #tatgcaca   6420  
               
               
                   
               
               
                 ctcgctgcca ctgtggagct gagatcactg gacatgtcaa aaacgggacg at  
               
               
                 #gaggatcg   6480  
               
               
                   
               
               
                 tcggtcctag gacctgcaag aacatgtgga gtgggacgtt cttcattaat gc  
               
               
                 #ctacacca   6540  
               
               
                   
               
               
                 cgggcccctg tactcccctt cctgcgccga actataagtt cgcgctgtgg ag  
               
               
                 #ggtgtctg   6600  
               
               
                   
               
               
                 cagaggaata cgtggagata aggcgggtgg gggacttcca ctacgtatcg gg  
               
               
                 #catgacta   6660  
               
               
                   
               
               
                 ctgacaatct caaatgcccg tgccagatcc catcgcccga atttttcaca ga  
               
               
                 #attggacg   6720  
               
               
                   
               
               
                 gggtgcgcct acataggttt gcgccccctt gcaagccctt gctgcgggag ga  
               
               
                 #ggtatcat   6780  
               
               
                   
               
               
                 tcagagtagg actccacgag tacccggtgg ggtcgcaatt accttgcgag cc  
               
               
                 #cgaaccgg   6840  
               
               
                   
               
               
                 acgtagccgt gttgacgtcc atgctcactg atccctccca tataacagca ga  
               
               
                 #ggcggccg   6900  
               
               
                   
               
               
                 ggagaaggtt ggcgagaggg tcaccccctt ctatggccag ctcctcggct ag  
               
               
                 #ccagctgt   6960  
               
               
                   
               
               
                 ccgctccatc tctcaaggca acttgcaccg ccaaccatga ctcccctgac gc  
               
               
                 #cgagctca   7020  
               
               
                   
               
               
                 tagaggctaa cctcctgtgg aggcaggaga tgggcggcaa catcaccagg gt  
               
               
                 #tgagtcag   7080  
               
               
                   
               
               
                 agaacaaagt ggtgattctg gactccttcg atccgcttgt ggcagaggag ga  
               
               
                 #tgagcggg   7140  
               
               
                   
               
               
                 aggtctccgt acccgcagaa attctgcgga agtctcggag attcgcccca gc  
               
               
                 #cctgcccg   7200  
               
               
                   
               
               
                 tctgggcgcg gccggactac aaccccctgc tagtagagac gtggaaaaag cc  
               
               
                 #tgactacg   7260  
               
               
                   
               
               
                 aaccacctgt ggtccatggc tgcccgctac cacctccacg gtcccctcct gt  
               
               
                 #gcctccgc   7320  
               
               
                   
               
               
                 ctcggaaaaa gcgtacggtg gtcctcaccg aatcaaccct acctactgcc tt  
               
               
                 #ggccgagc   7380  
               
               
                   
               
               
                 ttgccaccaa aagttttggc agctcctcaa cttccggcat tacgggcgac aa  
               
               
                 #tacgacaa   7440  
               
               
                   
               
               
                 catcctctga gcccgcccct tctggctgcc cccccgactc cgacgttgag tc  
               
               
                 #ctattctt   7500  
               
               
                   
               
               
                 ccatgccccc cctggagggg gagcctgggg atccggatct cagcgacggg tc  
               
               
                 #atggtcga   7560  
               
               
                   
               
               
                 cggtcagtag tggggccgac acggaagatg tcgtgtgctg ctcaatgtct ta  
               
               
                 #ttcctgga   7620  
               
               
                   
               
               
                 caggcgcact cgtcaccccg tgcgctgcgg aggaacaaaa actgcccatc aa  
               
               
                 #cgcactga   7680  
               
               
                   
               
               
                 gcaactcgtt gctacgccat cacaatctgg tgtattccac cacttcacgc ag  
               
               
                 #tgcttgcc   7740  
               
               
                   
               
               
                 aaaggaagaa gaaagtcaca tttgacagac tgcaagttct ggacagccat ta  
               
               
                 #ccaggacg   7800  
               
               
                   
               
               
                 tgctcaagga ggtcaaagca gcggcgtcaa aagtgaaggc taacttgcta tc  
               
               
                 #cgtagagg   7860  
               
               
                   
               
               
                 aagcttgcag cctggcgccc ccacattcag ccaaatccaa gtttggctat gg  
               
               
                 #ggcaaaag   7920  
               
               
                   
               
               
                 acgtccgttg ccatgccaga aaggccgtag cccacatcaa ctccgtgtgg aa  
               
               
                 #agaccttc   7980  
               
               
                   
               
               
                 tggaagacag tgtaacacca atagacacta ccatcatggc caagaacgag gt  
               
               
                 #tttctgcg   8040  
               
               
                   
               
               
                 ttcagcctga gaaggggggt cgtaagccag ctcgtctcat cgtgttcccc ga  
               
               
                 #cctgggcg   8100  
               
               
                   
               
               
                 tgcgcgtgtg cgagaagatg gccctgtacg acgtggttag caagctcccc tt  
               
               
                 #ggccgtga   8160  
               
               
                   
               
               
                 tgggaagctc ctacggattc caatactcac caggacagcg ggttgaattc ct  
               
               
                 #cgtgcaag   8220  
               
               
                   
               
               
                 cgtggaagtc caagaagacc ccgatggggc tctcgtatga tacccgctgt tt  
               
               
                 #tgactcca   8280  
               
               
                   
               
               
                 cagtcactga gagcgacatc cgtacggagg aggcaattta ccaatgttgt ga  
               
               
                 #cctggacc   8340  
               
               
                   
               
               
                 cccaagcccg cgtggccatc aagtccctca ctgagaggct ttatgttggg gg  
               
               
                 #ccctctta   8400  
               
               
                   
               
               
                 ctaattcaag gggggaaaac tgcggctacc gcaggtgccg cgcgagcaga gt  
               
               
                 #actgacaa   8460  
               
               
                   
               
               
                 ctagctgtgg taacaccctc actcgctaca tcaaggcccg ggcagcctgt cg  
               
               
                 #agccgcag   8520  
               
               
                   
               
               
                 ggctccagga ctgcaccatg ctcgtgtgtg gcgacgactt agtcgttatc tg  
               
               
                 #tgaaagtg   8580  
               
               
                   
               
               
                 cgggggtcca ggaggacgcg gcgagcctga gagccttcac ggaggctatg ac  
               
               
                 #caggtact   8640  
               
               
                   
               
               
                 ccgccccccc cggggacccc ccacaaccag aatacgactt ggagcttata ac  
               
               
                 #atcatgct   8700  
               
               
                   
               
               
                 cctccaacgt gtcagtcgcc cacgacggcg ctggaaagag ggtctactac ct  
               
               
                 #tacccgtg   8760  
               
               
                   
               
               
                 accctacaac ccccctcgcg agagccgcgt gggagacagc aagacacact cc  
               
               
                 #agtcaatt   8820  
               
               
                   
               
               
                 cctggctagg caacataatc atgtttgccc ccacactgtg ggcgaggatg at  
               
               
                 #actgatga   8880  
               
               
                   
               
               
                 cccacttctt tagcgtcctc atagccaggg atcagcttga acaggctctc aa  
               
               
                 #ctgcgaga   8940  
               
               
                   
               
               
                 tctacggagc ctgctactcc atagaaccac tggatctacc tccaatcatt ca  
               
               
                 #aagactcc   9000  
               
               
                   
               
               
                 atggcctcag cgcattttca ctccacagtt actctccagg tgaaattaat ag  
               
               
                 #ggtggccg   9060  
               
               
                   
               
               
                 catgcctcag aaaacttggg gtcccgccct tgcgagcttg gagacaccgg gc  
               
               
                 #ctggagcg   9120  
               
               
                   
               
               
                 tccgcgctag gcttctggcc agaggaggca aggctgccat atgtggcaag ta  
               
               
                 #cctcttca   9180  
               
               
                   
               
               
                 actgggcagt aagaacaaag ctcaaactca ctccgataac ggccgctggc cg  
               
               
                 #gctggact   9240  
               
               
                   
               
               
                 tgtccggctg gttcacggct ggctacagcg ggggagacat ttatcacagc gt  
               
               
                 #gtctcatg   9300  
               
               
                   
               
               
                 cccggccccg ctggttctgg ttttgcctac tcctgcttgc tgcaggggta gg  
               
               
                 #catctacc   9360  
               
               
                   
               
               
                 tcctccccaa ccgatgaaga ttgggctaac cactccaggc caataggcca tt  
               
               
                 #ccct       9416  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 14  
               
               
                 &lt;211&gt; LENGTH: 3182  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis B virus sequenc  
               
               
                 #e  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 14  
               
               
                   
               
               
                 aattccacaa ccttccacca aactctgcaa gatcccagag tgagaggcct gt  
               
               
                 #atttccct     60  
               
               
                   
               
               
                 gctggtggct ccagttcagg aacagtaaac cctgttctga ctactgcctc tc  
               
               
                 #ccttatcg    120  
               
               
                   
               
               
                 tcaatcttct cgaggattgg ggaccctgcg ctgaacatgg agaacatcac at  
               
               
                 #caggattc    180  
               
               
                   
               
               
                 ctaggacccc ttctcgtgtt acaggcgggg tttttcttgt tgacaagaat cc  
               
               
                 #tcacaata    240  
               
               
                   
               
               
                 ccgcagagtc tagactcgtg gtggacttct ctcaattttc tagggggaac ta  
               
               
                 #ccgtgtgt    300  
               
               
                   
               
               
                 cttggccaaa attcgcagtc cccaacctcc aatcactcac caacctcttg tc  
               
               
                 #ctccaact    360  
               
               
                   
               
               
                 tgtcctggtt atcgctggat gtgtctgcgg cgttttatca tcttcctctt ca  
               
               
                 #tcctgctg    420  
               
               
                   
               
               
                 ctatgcctca tcttcttgtt ggttcttctg gactatcaag gtatgttgcc cg  
               
               
                 #tttgtcct    480  
               
               
                   
               
               
                 ctaattccag gatcctcaac aaccagcacg ggaccatgcc ggacctgcat ga  
               
               
                 #ctactgct    540  
               
               
                   
               
               
                 caaggaacct ctatgtatcc ctcctgttgc tgtaccaaac cttcggacgg aa  
               
               
                 #attgcacc    600  
               
               
                   
               
               
                 tgtattccca tcccatcatc ctgggctttc ggaaaattcc tatgggagtg gg  
               
               
                 #cctcagcc    660  
               
               
                   
               
               
                 cgtttctcct ggctcagttt actagtgcca tttgttcagt ggttcgtagg gc  
               
               
                 #tttccccc    720  
               
               
                   
               
               
                 actgtttggc tttcagttat atggatgatg tggtattggg ggccaagtct gt  
               
               
                 #acagcatc    780  
               
               
                   
               
               
                 ttgagtccct ttttaccgct gttaccaatt ttcttttgtc tttgggtata ca  
               
               
                 #tttaaacc    840  
               
               
                   
               
               
                 ctaacaaaac aaagagatgg ggttactctc taaattttat gggttatgtc at  
               
               
                 #tggatgtt    900  
               
               
                   
               
               
                 atgggtcctt gccacaagaa cacatcatac aaaaaatcaa agaatgtttt ag  
               
               
                 #aaaacttc    960  
               
               
                   
               
               
                 ctattaacag gcctattgat tggaaagtat gtcaacgaat tgtgggtctt tt  
               
               
                 #gggttttg   1020  
               
               
                   
               
               
                 ctgccccttt tacacaatgt ggttatcctg cgttgatgcc tttgtatgca tg  
               
               
                 #tattcaat   1080  
               
               
                   
               
               
                 ctaagcaggc tttcactttc tcgccaactt acaaggcctt tctgtgtaaa ca  
               
               
                 #atacctga   1140  
               
               
                   
               
               
                 acctttaccc cgttgcccgg caacggccag gtctgtgcca agtgtttgct ga  
               
               
                 #cgcaaccc   1200  
               
               
                   
               
               
                 ccactggctg gggcttggtc atgggccatc agcgcatgcg tggaaccttt tc  
               
               
                 #ggctcctc   1260  
               
               
                   
               
               
                 tgccgatcca tactgcggaa ctcctagccg cttgttttgc tcgcagcagg tc  
               
               
                 #tggagcaa   1320  
               
               
                   
               
               
                 acattatcgg gactgataac tctgttgtcc tatcccgcaa atatacatcg tt  
               
               
                 #tccatggc   1380  
               
               
                   
               
               
                 tgctaggctg tgctgccaac tggatcctgc gcgggacgtc ctttgtttac gt  
               
               
                 #cccgtcgg   1440  
               
               
                   
               
               
                 cgctgaatcc tgcggacgac ccttctcggg gtcgcttggg actctctcgt cc  
               
               
                 #ccttctcc   1500  
               
               
                   
               
               
                 gtctgccgtt ccgaccgacc acggggcgca cctctcttta cgcggactcc cc  
               
               
                 #gtctgtgc   1560  
               
               
                   
               
               
                 cttctcatct gccggaccgt gtgcacttcg cttcacctct gcacgtcgca tg  
               
               
                 #gagaccac   1620  
               
               
                   
               
               
                 cgtgaacgcc caccaaatat tgcccaaggt cttacataag aggactcttg ga  
               
               
                 #ctctcagc   1680  
               
               
                   
               
               
                 aatgtcaacg accgaccttg aggcatactt caaagactgt ttgtttaaag ac  
               
               
                 #tgggagga   1740  
               
               
                   
               
               
                 gttgggggag gagattaggt taaaggtctt tgtactagga ggctgtaggc at  
               
               
                 #aaattggt   1800  
               
               
                   
               
               
                 ctgcgcacca gcaccatgca actttttcac ctctgcctaa tcatctcttg tt  
               
               
                 #catgtcct   1860  
               
               
                   
               
               
                 actgttcaag cctccaagct gtgccttggg tggctttggg gcatggacat cg  
               
               
                 #acccttat   1920  
               
               
                   
               
               
                 aaagaatttg gagctactgt ggagttactc tcgtttttgc cttctgactt ct  
               
               
                 #ttccttca   1980  
               
               
                   
               
               
                 gtacgagatc ttctagatac cgcctcagct ctgtatcggg aagccttaga gt  
               
               
                 #ctcctgag   2040  
               
               
                   
               
               
                 cattgttcac ctcaccatac tgcactcagg caagcaattc tttgctgggg gg  
               
               
                 #aactaatg   2100  
               
               
                   
               
               
                 actctagcta cctgggtggg tgttaatttg gaagatccag cgtctagaga cc  
               
               
                 #tagtagtc   2160  
               
               
                   
               
               
                 agttatgtca acactaatat gggcctaaag ttcaggcaac tcttgtggtt tc  
               
               
                 #acatttct   2220  
               
               
                   
               
               
                 tgtctcactt ttggaagaga aacagttata gagtatttgg tgtctttcgg ag  
               
               
                 #tgtggatt   2280  
               
               
                   
               
               
                 cgcactcctc cagcttatag accaccaaat gcccctatcc tatcaacact tc  
               
               
                 #cggagact   2340  
               
               
                   
               
               
                 actgttgtta gacgacgagg caggtcccct agaagaagaa ctccctcgcc tc  
               
               
                 #gcagacga   2400  
               
               
                   
               
               
                 aggtctcaat cgccgcgtcg cagaagatct caatctcggg aatctcaatg tt  
               
               
                 #agtattcc   2460  
               
               
                   
               
               
                 ttggactcat aaggtgggga actttactgg gctttattct tctactgtac ct  
               
               
                 #gtctttaa   2520  
               
               
                   
               
               
                 tcctcattgg aaaacaccat cttttcctaa tatacattta caccaagaca tt  
               
               
                 #atcaaaaa   2580  
               
               
                   
               
               
                 atgtgaacag tttgtaggcc cactcacagt taatgagaaa agaagattgc aa  
               
               
                 #ttgattat   2640  
               
               
                   
               
               
                 gcctgccagg ttttatccaa aggttaccaa atatttacca ttggataagg gt  
               
               
                 #attaaacc   2700  
               
               
                   
               
               
                 ttattatcca gaacatctag ttaatcatta cttccaaact agacactatt ta  
               
               
                 #cacactct   2760  
               
               
                   
               
               
                 atggaaggcg ggtatattat ataagagaga aacaacacat agcgcctcat tt  
               
               
                 #tgtgggtc   2820  
               
               
                   
               
               
                 accatattct tgggaacaag atctacagca tggggcagaa tctttccacc ag  
               
               
                 #caatcctc   2880  
               
               
                   
               
               
                 tgggattctt tcccgaccac cagttggatc cagccttcag agcaaacacc gc  
               
               
                 #aaatccag   2940  
               
               
                   
               
               
                 attgggactt caatcccaac aaggacacct ggccagacgc caacaaggta gg  
               
               
                 #agctggag   3000  
               
               
                   
               
               
                 cattcgggct gggtttcacc ccaccgcacg gaggcctttt ggggtggagc cc  
               
               
                 #tcaggctc   3060  
               
               
                   
               
               
                 agggcatact acaaactttg ccagcaaatc cgcctcctgc ctccaccaat cg  
               
               
                 #ccagtcag   3120  
               
               
                   
               
               
                 gaaggcagcc taccccgctg tctccacctt tgagaaacac tcatcctcag gc  
               
               
                 #catgcagt   3180  
               
               
                   
               
               
                 gg                   
               
               
                 #                   
               
               
                 #                   
               
               
                 #            3182  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 15  
               
               
                 &lt;211&gt; LENGTH: 7478  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: Hepatitis A virus sequenc  
               
               
                 #e  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 15  
               
               
                   
               
               
                 ttcaagaggg gtctccggag gtttccggag cccctcttgg aagtccatgg tg  
               
               
                 #aggggact     60  
               
               
                   
               
               
                 tgatacctca ccgccgtttg cctaggctat aggctaaatt tccctttccc tg  
               
               
                 #tccctccc    120  
               
               
                   
               
               
                 ttatttccct ttgttttgct tgtaaatatt aattcctgca ggttcagggt tc  
               
               
                 #tttaatct    180  
               
               
                   
               
               
                 gtttctctat aagaacactc aattttcacg ctttctgtct tctttcttcc ag  
               
               
                 #ggctctcc    240  
               
               
                   
               
               
                 ccttgcccta ggctctggcc gttgcgcccg gcggggtcaa ctccatgatt ag  
               
               
                 #catggagc    300  
               
               
                   
               
               
                 tgtaggagtc taaattgggg acgcagatgt ttgggacgtc accttgcagt gt  
               
               
                 #taacttgg    360  
               
               
                   
               
               
                 ctctcatgaa cctctttgat cttccacaag gggtaggcta cgggtgaaac ct  
               
               
                 #cttaggct    420  
               
               
                   
               
               
                 aatacttcta tgaagagatg ctttggatag ggtaacagcg gcggatattg gt  
               
               
                 #gagttgtt    480  
               
               
                   
               
               
                 aagacaaaaa ccattcaacg ccggaggact ggctctcatc cagtggatgc at  
               
               
                 #tgagtgga    540  
               
               
                   
               
               
                 ttgattgtca gggctgtctc taggtttaat ctcagacctc tctgtgctta gg  
               
               
                 #gcaaacac    600  
               
               
                   
               
               
                 catttggcct taaatgggat cctgtgagag ggggtccctc cattgacagc tg  
               
               
                 #gactgttc    660  
               
               
                   
               
               
                 tttggggcct tatgtggtgt ttgcctctga ggtactcagg ggcatttagg tt  
               
               
                 #tttcctca    720  
               
               
                   
               
               
                 ttcttaaaca ataatgaata tgtccaaaca aggaattttc cagactgttg gg  
               
               
                 #agtggcct    780  
               
               
                   
               
               
                 tgaccacatc ctgtctttgg cagatattga ggaagagcaa atgattcagt cc  
               
               
                 #gttgatag    840  
               
               
                   
               
               
                 gactgcagtg actggagctt cttacttcac ttctgtggac caatcttcag tt  
               
               
                 #catactgc    900  
               
               
                   
               
               
                 tgaggttggc tcacatcaaa ttgaaccttt gaaaacctct gttgataaac ct  
               
               
                 #ggttctaa    960  
               
               
                   
               
               
                 gaaaactcag ggggaaaagt ttttcctgat tcattctgct gattggctca ct  
               
               
                 #acacatgc   1020  
               
               
                   
               
               
                 tctctttcat gaagttgcaa aattggatgt ggtgaaacta ctgtataatg ag  
               
               
                 #cagtttgc   1080  
               
               
                   
               
               
                 cgtccaaggt ttgttgagat accatacata tgcaagattt ggcattgaga tt  
               
               
                 #caagttca   1140  
               
               
                   
               
               
                 gataaatccc acaccctttc agcaaggagg actaatttgt gccatggttc ct  
               
               
                 #ggtgacca   1200  
               
               
                   
               
               
                 aagttatggt tcaatagcat ccttgactgt ttatcctcat ggtctgttaa at  
               
               
                 #tgcaatat   1260  
               
               
                   
               
               
                 caacaatgta gttagaataa aggttccatt tatttatact agaggtgctt at  
               
               
                 #cattttaa   1320  
               
               
                   
               
               
                 agatccacag tacccagttt gggaattgac aatcagagtt tggtcagagt tg  
               
               
                 #aatattgg   1380  
               
               
                   
               
               
                 aacaggaact tcagcttaca cttcactcaa tgttttagct aggtttacag at  
               
               
                 #ttggagtt   1440  
               
               
                   
               
               
                 gcatggatta actcctcttt ctacacagat gatgagaaat gaatttaggg tc  
               
               
                 #agtactac   1500  
               
               
                   
               
               
                 tgaaaatgtt gtaaatttgt caaattatga agatgcaagg gcaaaaatgt ct  
               
               
                 #tttgcttt   1560  
               
               
                   
               
               
                 ggatcaggaa gattggaagt ctgatccttc ccaaggtggt ggaattaaaa tt  
               
               
                 #actcattt   1620  
               
               
                   
               
               
                 tactacctgg acatccattc caaccttagc tgctcagttt ccatttaatg ct  
               
               
                 #tcagattc   1680  
               
               
                   
               
               
                 agttggacaa caaattaaag ttattccagt ggacccatac tttttccaaa tg  
               
               
                 #acaaacac   1740  
               
               
                   
               
               
                 taatcctgat caaaaatgta taactgcctt ggcctctatt tgtcagatgt tc  
               
               
                 #tgcttttg   1800  
               
               
                   
               
               
                 gaggggagat cttgtttttg attttcaggt ttttccaacc aaatatcatt ca  
               
               
                 #ggtagact   1860  
               
               
                   
               
               
                 gttgttttgt tttgttcctg ggaatgagtt aatagatgtt actggaatta ca  
               
               
                 #ttaaaaca   1920  
               
               
                   
               
               
                 ggcaactact gctccttgtg cagtgatgga cattacagga gtgcagtcaa cc  
               
               
                 #ttgagatt   1980  
               
               
                   
               
               
                 tcgtgttcct tggatttctg atacacctta tcgagtgaat aggtacacga ag  
               
               
                 #tcagcaca   2040  
               
               
                   
               
               
                 tcaaaaaggt gagtacactg ccattgggaa gcttattgtg tattgttata ac  
               
               
                 #agactgac   2100  
               
               
                   
               
               
                 ttctccttct aatgttgcct ctcatgttag agttaatgtt tatctttcag ca  
               
               
                 #attaattt   2160  
               
               
                   
               
               
                 ggaatgtttt gctcctcttt accatgctat ggatgttact acacaggttg ga  
               
               
                 #gatgattc   2220  
               
               
                   
               
               
                 aggaggtttc tcaacaacag tttctacaga gcagaatgtt cctgatcccc aa  
               
               
                 #gttgggat   2280  
               
               
                   
               
               
                 aacaaccatg agggatttaa aaggaaaagc caatagggga aagatggatg tt  
               
               
                 #tcaggagt   2340  
               
               
                   
               
               
                 gcaagcacct cgtgggagct atcagcaaca attgaacgat ccagttttag ca  
               
               
                 #aagaaagt   2400  
               
               
                   
               
               
                 acctgagaca tttcctgaat tgaagcctgg agagtccaga catacatcag at  
               
               
                 #cacatgtc   2460  
               
               
                   
               
               
                 tatttataaa ttcatgggaa ggtctcattt tttgtgcact tttactttca at  
               
               
                 #tcaaataa   2520  
               
               
                   
               
               
                 taaagagtac acatttccaa taaccctgtc ttcgacttct aatcctcctc at  
               
               
                 #ggtttacc   2580  
               
               
                   
               
               
                 atcaacatta aggtggttct tcaatttgtt tcagttgtat agaggaccat tg  
               
               
                 #gatttaac   2640  
               
               
                   
               
               
                 aattataatc acaggagcca ctgatgtgga tggtatggcc tggtttactc ca  
               
               
                 #gtgggcct   2700  
               
               
                   
               
               
                 tgctgtcgac ccttgggtgg aaaaggagtc agctttgtct attgattata aa  
               
               
                 #actgccct   2760  
               
               
                   
               
               
                 tggagctgtt agatttaata caagaagaac aggaaacatt caaattagat tg  
               
               
                 #ccgtggta   2820  
               
               
                   
               
               
                 ttcttatttg tatgccgtgt ctggagcact ggatggcttg ggggataaga ca  
               
               
                 #gattctac   2880  
               
               
                   
               
               
                 atttggattg tttctattcg agattgcaaa ttacaatcat tctgatgaat at  
               
               
                 #ttgtcctt   2940  
               
               
                   
               
               
                 cagttgttat ttgtctgtca cagagcaatc agagttctat tttcctagag ct  
               
               
                 #ccattaaa   3000  
               
               
                   
               
               
                 ttcaaatgct atgttgtcca ctgaatccat gatgagtaga attgcagctg ga  
               
               
                 #gacttgga   3060  
               
               
                   
               
               
                 gtcatcagtg gatgatccca gatcagagga ggatagaaga tttgagagtc at  
               
               
                 #atagaatg   3120  
               
               
                   
               
               
                 taggaaacca tacaaagaat tgagactgga ggttgggaaa caaagactca aa  
               
               
                 #tatgctca   3180  
               
               
                   
               
               
                 ggaagagtta tcaaatgaag tgcttccacc tcctaggaaa atgaaggggt ta  
               
               
                 #ttttcaca   3240  
               
               
                   
               
               
                 agctaaaatt tctctttttt atactgagga gcatgaaata atgaagtttt ct  
               
               
                 #tggagagg   3300  
               
               
                   
               
               
                 agtgactgct gatactaggg ctttgagaag atttggattc tctctggctg ct  
               
               
                 #ggtagaag   3360  
               
               
                   
               
               
                 tgtgtggact cttgaaatgg atgctggagt tcttactgga agattgatca ga  
               
               
                 #ttgaatga   3420  
               
               
                   
               
               
                 tgagaaatgg acagaaatga aggatgataa gattgtttca ttaattgaaa ag  
               
               
                 #ttcacaag   3480  
               
               
                   
               
               
                 caataaatat tggtctaaag tgaattttcc acatggaatg ttggatcttg aa  
               
               
                 #gaaattgc   3540  
               
               
                   
               
               
                 tgccaattct aaggattttc caaatatgtc tgagacagat ttgtgtttcc tg  
               
               
                 #ttacattg   3600  
               
               
                   
               
               
                 gctaaatcca aagaaaatca atttagcaga tagaatgctt ggattgtctg ga  
               
               
                 #gtgcagga   3660  
               
               
                   
               
               
                 aattaaggaa cagggtgttg gactgatagc agagtgtaga actttcttgg at  
               
               
                 #tctattgc   3720  
               
               
                   
               
               
                 tgggactttg aaatctatga tgtttgggtt tcatcattct gtgactgttg aa  
               
               
                 #attataaa   3780  
               
               
                   
               
               
                 tactgtgctt tgttttgtta agagtggaat cctgctttat gtcatacaac aa  
               
               
                 #ttgaacca   3840  
               
               
                   
               
               
                 agatgaacac tctcacataa ttggtttgtt gagagttatg aattatgcag at  
               
               
                 #attggctg   3900  
               
               
                   
               
               
                 ttcagttatt tcatgtggta aagttttttc caaaatgtta gaaacagttt tt  
               
               
                 #aattggca   3960  
               
               
                   
               
               
                 aatggattct agaatgatgg agctgaggac tcagagcttc tctaattggt ta  
               
               
                 #agagatat   4020  
               
               
                   
               
               
                 ttgttcagga attactattt ttaaaagttt taaggatgcc atatattggt ta  
               
               
                 #tatacaaa   4080  
               
               
                   
               
               
                 attgaaggat ttttatgaag taaattatgg caagaaaaag gatattctta at  
               
               
                 #attctcaa   4140  
               
               
                   
               
               
                 agataatcag caaaaaatag aaaaagccat tgaagaagca gacaattttt gc  
               
               
                 #attttgca   4200  
               
               
                   
               
               
                 aattcaagat gtagagaaat ttgatcagta tcagaaaggg gttgatttaa ta  
               
               
                 #caaaagct   4260  
               
               
                   
               
               
                 gagaactgtc cattcaatgg cgcaagttga ccccaatttg ggggttcatt tg  
               
               
                 #tcacctct   4320  
               
               
                   
               
               
                 cagagattgc atagcaagag tccaccaaaa gctcaagaat cttggatcta ta  
               
               
                 #aatcaggc   4380  
               
               
                   
               
               
                 catggtaaca agatgtgagc cagttgtttg ctatttgtat ggcaaaagag gg  
               
               
                 #ggagggaa   4440  
               
               
                   
               
               
                 aagcttgact tcaattgcat tggcaaccaa aatttgtaaa cactatggtg tt  
               
               
                 #gaacctga   4500  
               
               
                   
               
               
                 gaaaaatatt tacaccaaac ctgtggcctc agattattgg gatggatata gt  
               
               
                 #ggacaatt   4560  
               
               
                   
               
               
                 agtttgcatt attgatgata ttggccaaaa cacaacagat gaagattggt ca  
               
               
                 #gatttttg   4620  
               
               
                   
               
               
                 tcaattagtg tcaggatgcc caatgagatt gaatatggct tctctagagg ag  
               
               
                 #aagggcag   4680  
               
               
                   
               
               
                 acatttttcc tctcctttta taatagcaac ttcaaattgg tcaaatccaa gt  
               
               
                 #ccaaaaac   4740  
               
               
                   
               
               
                 agtttatgtt aaggaagcaa ttgatcgtag gcttcatttt aaggttgaag tt  
               
               
                 #aaacctgc   4800  
               
               
                   
               
               
                 ttcatttttt aaaaatcctc acaatgatat gttgaatgtt aatttggcca aa  
               
               
                 #acaaatga   4860  
               
               
                   
               
               
                 tgcaattaag gacatgtctt gtgttgattt aataatggat ggacacaata tt  
               
               
                 #tcattgat   4920  
               
               
                   
               
               
                 ggatttactt agttccttag tgatgacagt tgaaattagg aaacagaata tg  
               
               
                 #agtgaatt   4980  
               
               
                   
               
               
                 catggagttg tggtctcagg gaatttcaga tgatgacaat gatagtgcag tg  
               
               
                 #gctgagtt   5040  
               
               
                   
               
               
                 tttccagtct tttccatctg gtgaaccatc aaattggaag ttatctagtt tt  
               
               
                 #ttccaatc   5100  
               
               
                   
               
               
                 tgtcactaat cacaagtggg ttgctgtggg agctgcagtt ggcattcttg ga  
               
               
                 #gtgcttgt   5160  
               
               
                   
               
               
                 gggaggatgg tttgtgtata agcatttttc ccgcaaagag gaagaaccaa tt  
               
               
                 #ccagctga   5220  
               
               
                   
               
               
                 aggggtttat catggcgtga ctaagcccaa acaagtgatt aaattggatg ca  
               
               
                 #gatccagt   5280  
               
               
                   
               
               
                 agagtcccag tcaactctag aaatagcagg attagttagg aaaaatctgg tt  
               
               
                 #cagtttgg   5340  
               
               
                   
               
               
                 agttggtgag aaaaatggat gtgtgagatg ggtcatgaat gccttaggag tg  
               
               
                 #aaggatga   5400  
               
               
                   
               
               
                 ttggttgtta gtaccttctc atgcttataa atttgaaaag gattatgaaa tg  
               
               
                 #atggagtt   5460  
               
               
                   
               
               
                 ttacttcaat agaggtggaa cttactattc aatttcagct ggtaatgttg tt  
               
               
                 #attcaatc   5520  
               
               
                   
               
               
                 tttagatgtg ggatttcaag atgttgtttt aatgaaggtt cctacaattc cc  
               
               
                 #aagtttag   5580  
               
               
                   
               
               
                 agatattact caacacttta ttaagaaagg agatgtgcct agagccttaa at  
               
               
                 #cgcttggc   5640  
               
               
                   
               
               
                 aacattagtg acaaccgtta atggaactcc tatgttaatt tctgagggac ca  
               
               
                 #ttaaagat   5700  
               
               
                   
               
               
                 ggaagaaaaa gccacttatg ttcataagaa gaatgatggt actacagttg at  
               
               
                 #ttgactgt   5760  
               
               
                   
               
               
                 agatcaggca tggagaggaa aaggtgaagg tcttcctgga atgtgtggtg gg  
               
               
                 #gccctagt   5820  
               
               
                   
               
               
                 gtcatcaaat cagtccatac agaatgcaat tttgggtatt catgttgctg ga  
               
               
                 #ggaaattc   5880  
               
               
                   
               
               
                 aattcttgtg gcaaagctgg ttactcaaga aatgtttcaa aacattgata ag  
               
               
                 #aaaattga   5940  
               
               
                   
               
               
                 aagtcagaga ataatgaaag tggaatttac tcaatgttca atgaatgtag tc  
               
               
                 #tccaaaac   6000  
               
               
                   
               
               
                 gctttttaga aagagtccca ttcatcacca cattgataaa accatgatta at  
               
               
                 #tttcctgc   6060  
               
               
                   
               
               
                 agctatgcct ttctctaaag ctgaaattga tccaatggct atgatgttgt cc  
               
               
                 #aaatattc   6120  
               
               
                   
               
               
                 attacctatt gtggaggaac cagaggatta caaggaagct tcagtttttt at  
               
               
                 #caaaacaa   6180  
               
               
                   
               
               
                 aatagtaggc aagactcagc tagttgatga ctttttagat cttgatatgg ct  
               
               
                 #attacagg   6240  
               
               
                   
               
               
                 ggctccaggc attgatgcta tcaatatgga ttcatctcct gggtttcctt at  
               
               
                 #gttcaaga   6300  
               
               
                   
               
               
                 aaaattgacc aaaagagatt taatttggtt ggatgaaaat ggtttgctgt ta  
               
               
                 #ggagttca   6360  
               
               
                   
               
               
                 cccaagattg gcccagagaa ttttatttaa tactgtcatg atggaaaatt gt  
               
               
                 #tctgactt   6420  
               
               
                   
               
               
                 agatgttgtt tttacaactt gtccaaaaga tgaattgaga ccattagaga aa  
               
               
                 #gttttgga   6480  
               
               
                   
               
               
                 atcaaaaaca agagccattg atgcttgtcc tttggattat acaattctat gt  
               
               
                 #cgaatgta   6540  
               
               
                   
               
               
                 ttggggtcca gctatcagtt atttccattt gaatccaggg tttcacacag gt  
               
               
                 #gttgctat   6600  
               
               
                   
               
               
                 tggcatagat cctgatagac agtgggatga attatttaaa acaatgataa ga  
               
               
                 #tttggaga   6660  
               
               
                   
               
               
                 tgttggtctt gatttagatt tctctgcttt tgatgccagt cttagtccat tt  
               
               
                 #atgattag   6720  
               
               
                   
               
               
                 ggaagcaggt agaatcatga gtgaattatc tggaacacca tctcattttg ga  
               
               
                 #acagctct   6780  
               
               
                   
               
               
                 tatcaatact atcatttatt ctaaacatct gctgtacaac tgttgttatc at  
               
               
                 #gtttgtgg   6840  
               
               
                   
               
               
                 ttcaatgcct tctgggtctc cttgcacagc tttgttgaat tcaattatta at  
               
               
                 #aatattaa   6900  
               
               
                   
               
               
                 tctgtattat gtgttttcta aaatatttgg aaagtctcca gttttctttt gt  
               
               
                 #caagcttt   6960  
               
               
                   
               
               
                 gaggatcctt tgttacggag atgatgtttt gatagttttt tccagagatg tt  
               
               
                 #caaattga   7020  
               
               
                   
               
               
                 caatcttgac ttgattggac agaaaattgt agatgagttc aaaaaacttg gc  
               
               
                 #atgacagc   7080  
               
               
                   
               
               
                 cacctcagct gataaaaatg tgcctcaact gaagccagtt tcagaattga ct  
               
               
                 #tttctcaa   7140  
               
               
                   
               
               
                 aagatctttc aatttggtgg aggatagaat tagacctgca atttcagaaa ag  
               
               
                 #acaatttg   7200  
               
               
                   
               
               
                 gtctttgatg gcttggcaga gaagtaacgc tgagtttgag cagaatttag aa  
               
               
                 #aatgctca   7260  
               
               
                   
               
               
                 gtggtttgct tttatgcatg gctatgagtt ctatcagaaa ttttattatt tt  
               
               
                 #gttcagtc   7320  
               
               
                   
               
               
                 ctgtttggag aaagagatga tagaatatag acttaaatct tatgattggt gg  
               
               
                 #agaatgag   7380  
               
               
                   
               
               
                 attttatgac cagtgtttca tttgtgacct ttcatgattt gtttaaacaa at  
               
               
                 #tttcttac   7440  
               
               
                   
               
               
                 tctttctgag gtttgtttat ttcttttgtc cgctaact       
               
               
                 #                   
               
               
                 #   7478