Abstract:
The present invention relates to the field of a medicine for treating diseases associated with inflammation, immunity or infection, and in particular, to glycyrrhetinic acid-30-amide derivatives of general formula I and their preparation, and a pharmaceutical composition containing the same. Said derivatives and composition exhibit anti-inflammatory, analgesic, anti-allergic, cough-preventing, liver-protecting and anti-viral properties, 
     
       
                 
         
             
             
         
       
     
     wherein each group is as defined in the description.

Description:
TECHNICAL FIELD  
       [0001]    The present invention relates to the pharmaceutical field associated with inflammation, immunity or infection, and in particular, to glycyrrhetinic acid-30-amide derivatives exhibiting anti-inflammatory, analgesic, anti-allergic, cough-preventing, liver-protecting and anti-viral properties and their preparation processes, and pharmaceutical compositions containing them. 
       BACKGROUND OF THE INVENTION 
       [0002]    Glycyrrhizic acid and glycyrrhetinic acid have effects in relieving or eliminating inflammation, pain, allergy, and ulcer, protecting against virus, improving immunity, protecting liver and the like. Glycyrrhizic acid in injection form is now widely used for the clinical treatment of hepatitis; Carbenoxolone Sodium and zinc glycyrrhizate are used for treating gastric ulcer; and glycyrrhetinic acid in injection form is used for treating Addison&#39;s disease. Glycyrrhetinic acid, however, has a chemical structure partially similar to adrenal cortical hormone and thus produces side effects, typical of hormon pharmaceuticals, mainly aldosterone-like effects while used clinically in a large amount. These side effects lead to sodium retention and increased potassium excretion, thus resulting in edema, hypertension, hypopotassemia, etc. See Liang Qing, Pseudo-aldosteronosis caused by glycyrrhizic acid, Chinese Traditional and Herbal Drugs Communication, 1979, 6: 45-46; Wu P., Zhang Y, Liu Y, Effects of glycyrrhizin on production of vascular aldosterone and corticosterone, Horm—Res., 1999, 51 (4): 189-192. 
       SUMMARY OF THE INVENTION 
       [0003]    It is an object of the invention to provide a compound having general formula I and a pharmaceutically acceptable salt thereof, which displays higher pharmaceutical activity and reduced side effects, and thus overcome the defects and the disadvantages of the prior art. 
         [0004]    It is another object of the invention to provide a process for preparing the compound of general formula I and a pharmaceutically acceptable salt thereof. 
         [0005]    It is still another object of the invention to provide a pharmaceutical composition containing the compound of general formula I or a pharmaceutically acceptable salt thereof as active ingredient and a pharmaceutically acceptable carrier(s), excipient(s) or diluent(s), and use thereof in anti-inflammation, analgesia, anti-allergy, prevention of cough, protection of liver, anti-virus, etc. 
         [0006]    The invention will be described in details below in view of the object thereof. 
         [0007]    The compound of general formula I of the invention has the following structure: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0008]    wherein
       R 1  is halogen, —OH, —OR 1 ′, —OCOR 1 ′, —OCOCH 2 CH 2 COOH, —OCOCH 2 CH 2 COOR 1 ′, —NH 2 , —NHR 1 ′, —N(R 1 ′) 2 , —NHCOR 1 ′, —O(CH 2 ) 1-3 COOH, or —O(CH 2 ) 1-3 COOR 1 ′, wherein R 1 ′ is C 1 -C 5 -alkyl;   R 2  is H;
           —CH 2 R 2 ′, wherein R 2 ′ is hydrogen, halogen, hydroxyl, cyano, carboxyl, C 1 -C 8 -alkoxy, C 1 -C 5 -alkyl, or halogen-substituted C 1 -C 5 -alkyl;   phenyl, or phenyl which is mono- or poly-substituted by halogen, hydroxyl, cyano,   carboxyl, carboxy-C 1 -C 3 -alkyl, C 1 -C 8 -alkyl, amino, nitro, C 1 -C 8 -alkylamino or di(C 1 -C 8 -alkyl)amino, C 1 -C 8 -alkoxy, C 1 -C 5 -alkyl optionally substituted by halogen, or C 1 -C 8 -alkylcarbonyl; or   5- or 6-membered heterocyclic group containing sulphur, oxygen or nitrogen as heteroatom, or 5- or 6-membered heterocyclic group which is mono- or poly-substituted by halogen, hydroxyl, cyano, carboxyl, carboxy-C 1 -C 3 -alkyl, C 1 -C 8 -alkyl, amino, nitro, C 1 -C 8 -alkoxy, or C 1 -C 8 -alkylcarbonyl group;   
           X is CH 2  or C═O; and       
 
         [0016]    hydrogen in position 18 can be in R- or S-stereoisomer. 
         [0017]    Preferred compound of formula I or a pharmaceutically acceptable salt thereof are those wherein
       R 1  is fluoro, chloro, bromo, —OH, —OR 1 ′, —OCOR 1 ′, —OCOCH 2 CH 2 COOH, —OCOCH 2 CH 2 COOR 1 ′, —NH 2 , —NHR 1 ′, —N(R 1 ′) 2 , —NHCOR 1 , —OCH 2 COOH or —OCH 2 COOR 1 ′, wherein R 1 ′ is —CH 3 , -CH 2 CH 3 , —CH 2 CH 2 CH 3  or —CH(CH 3 ) 2 ;   R 2  is H;
           CH 2 R 2 ′, wherein R 2 ′ is hydrogen, fluoro, chloro, bromo, chloromethyl, chloroethyl,   hydroxyl, cyano, carboxyl, methoxy, ethoxy, n-propoxy, iso-propoxy, methyl, ethyl, n-propyl or iso-propyl group;   phenyl, or phenyl which is mono- or di-substituted by fluoro, chloro, bromo, hydroxyl,   cyano, carboxyl, carboxymethyl, amino, nitro, methoxy, ethoxy, iso-propoxy, methylamino, ethylamino, isopropylamino, butylamino, dimethylamino, diethylamino, methyl, ethyl, n-propyl, iso-propyl, acetyl, propionyl, or trifluoromethyl group; or imidazolyl, pyridinyl, oxazolyl, isoxazolyl, furyl, thiazolyl, pyrazolyl, thienyl, pyrrolyl,   pyridazinyl, pyrimidinyl, or pyrazinyl, or imidazolyl, pyridinyl, oxazolyl, isoxazolyl,   furyl, thiazolyl, pyrazolyl, thienyl, pyrrolyl, pyridazinyl, pyrimidinyl, or pyrazinyl which is each mono- or di-substituted by fluoro, chloro, bromo, hydroxyl, cyano, carboxyl, carboxymethyl, amino, nitro, methoxy, ethoxy, iso-propoxy, methylamino, ethylamino, isopropylamino, butylamino, methyl, ethyl, n-propyl, iso-propyl, acetyl, propionyl, or trifluoromethyl group;   
           X is CH 2  or C═O; and       
 
         [0027]    hydrogen in position 18 is in R (18-β isomer) or S (18-α isomer) configuration. 
         [0028]    More preferred compounds of formula I are shown in the following table. In the table, all the compounds are in the form of 18-β isomers (naturally occurring configuration) unless indicated otherwise. 
         [0000]    
       
         
               
               
             
           
               
                   
               
               
                 No. 
                 Nomenclature of the compound 
               
               
                   
               
             
             
               
                 G1 
                 N-[(3-p-hydroxylphenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide 
               
               
                 G2 
                 N-[(3-p-methylphenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide 
               
               
                 G3 
                 N-[(3-p-fluorophenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide 
               
               
                 G4 
                 N-[(3-o-chlorophenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide 
               
               
                 G5 
                 N-[(3-p-methoxyphenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide 
               
               
                 G6 
                 N-[(3-o-methoxyphenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide 
               
               
                 G7 
                 N-[(3-methyl-isoxazol-5-yl) methyl]-glycyrrhetinamide 
               
               
                 G8 
                 18-α, N-[(3-p-chlorophenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide 
               
               
                 G9 
                 N-[(3-p-trifluoromethylphenyl-isoxazol-5-yl)methyl]-glycyrrhetinamide 
               
               
                 G10 
                 N-[(3-phenyl-isoxazol-5-yl)methyl]-glycyrrhetinamide 
               
               
                 DG1 
                 N-[(3-p-hydroxylphenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG2 
                 N-[(3-p-methylphenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG3 
                 N-[(3-p-fluorophenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG4 
                 N-[(3-o-chlorophenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG5 
                 N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-chloro-11-deoxy-glycyrrhetinamide 
               
               
                 DG6 
                 N-[(3-o-methoxyphenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG7 
                 N-[(3-methyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG8 
                 N-[(3-m-chlorophenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG9 
                 N-[(3-p-acetylphenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG10 
                 18-α, N-[(3-p-nitrophenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG11 
                 N-[(3-(4-pyridin)yl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG12 
                 N-{[3-(4-chloroimidazol)-5-yl-isoxazol-5-yl]methyl}-11-deoxy-glycyrrhetinamide 
               
               
                 DG13 
                 N-{[3-(2,4-dichlorphenyl)-isoxazol-5-yl]methyl}-11-deoxy-glycyrrhetinamide 
               
               
                 DG14 
                 N-{[3-(2,4-dimethoxyphenyl)-isoxazol-5-yl]methyl}-11-deoxy-glycyrrhetinamide 
               
               
                 DG15 
                 N-[(3-p-trifluoromethylphenyl-isoxazol-5-yl) methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 DG16 
                 N-[(3-phenyl-isoxazol-5-yl)methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 RG1 
                 N-[(3-o-methoxyphenyl-isoxazol-5-yl) methyl]-3-acetoxy-glycyrrhetinamide 
               
               
                 RG2 
                 N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]-3-carboxymethoxy-glycyrrhetinamide 
               
               
                 RG3 
                 N-[(3-o-chlorophenyl-isoxazol-5-yl) methyl]-3-ethoxy-glycyrrhetinamide 
               
               
                 ADG1 
                 N-[(3-p-methoxyphenyl-isoxazol-5-yl) methyl]-3-amino-11-deoxy-glycyrrhetinamide 
               
               
                 ADG2 
                 N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-diethylamino-11-deoxy-glycyrrhetinamide 
               
               
                 ADG3 
                 N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-acetylamino-11-deoxy-glycyrrhetinamide 
               
               
                 YRG1 
                 Sodium {N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]glycyrrhetinamide-3-oxy}- 
               
               
                   
                 acetate 
               
               
                 YADG1 
                 N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-ammonium-11-deoxy-glycyrrhetinamide 
               
               
                   
                 hydrochloride 
               
               
                 YADG2 
                 N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-ammonium-11-deoxy-glycyrrhetinamide 
               
               
                   
                 acetate 
               
               
                 YADG3 
                 Triethylammonium {N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]glycyrrhetinamide-3-oxy}- 
               
               
                   
                 acetate 
               
               
                   
               
             
          
         
       
     
         [0029]    The compound of formula I according to the invention can be synthesized through the following steps: 
         [0030]    1. Reacting a compound of formula IIa with zinc-amalgam in the presence of dioxane and hydrochloric acid to obtain a compound of formula IIb, which can optionally be further converted to a compound of formula IIc: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0031]    2. Reacting a compound of formula IIIa, R 2 C═NOH, with an N-halosuccimide or sodium hypochlorite, and then, in the presence of a base (e.g. triethylamine, TEA), with propargyl amine to obtain a compound of formula IIId in a direct way; or 
         [0032]    reacting a compound of formula IIIa, R 2 C═NOH, with an N-halosuccimide or sodium hypochlorite, and then, in the presence of a base (e.g. TEA, etc.), with propargyl alcohol to produce a compound of formula IIIb, which is subjected to bromination to produce a compound of formula IIIc, which is in turn subjected to an aminolysis reaction to obtain a compound of formula IIId; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0033]    wherein, R 2  is as defined above, and the compounds of formula IIIa are commercially available or can be prepared by known processes; 
         [0034]    3. Reacting a compound of formula IIId with the compound of formula IIIa or IIIb to obtain a compound of formula Ia, 
         [0000]    
       
                 
         
             
             
         
       
     
         [0035]    wherein X and R 2  are as defined above; or 
         [0036]    reacting the compound of formula IIId with the compound of formula IIc to obtain a compound of formula Ib 
         [0000]    
       
                 
         
             
             
         
       
     
         [0037]    wherein X and R 2  are as defined above. 
         [0038]    By using the above compounds of formula I (Ia, or Ib), other compounds of formula I can be prepared by reacting compound Ia with an acyl halide, a bromoalkane, a carboxy-containing compound, or other reagents, or by reacting compound Ib with ammonia, an amine, an alcohol or other reagents. 
         [0039]    The pharmaceutically acceptable salts of the compounds of formula I of the invention include, but not being limited to, for example, sodium, potassium or calcium salt, which are formed with a basic compound such as sodium hydroxide, potassium hydroxide, calcium hydroxide, sodium carbonate, etc.; those formed with a suitable organic base such as methylamine, triethylamine or meglumine; those with an inorganic acid such as hydrochloric acid, sulphuric acid, nitric acid, phosphoric acid and the like; or those with an organic acid such as formic acid, acetic acid, citric acid, oxalic acid, fumaric acid, maleic acid, an amino acid and the like. 
         [0040]    The compounds of formula I and the salts thereof can be used as active ingredient in, for example, an anti-inflammatory agent, an anti-allergic agent, an analgesic agent, a cough-preventing agent, an anti-ulcer agent, an immunity-improving agent, a liver-protecting agent or an anti-virus agent. 
         [0041]    The compounds of formula I and the pharmaceutically acceptable salts thereof can be formulated into a pharmaceutical composition with one or more pharmaceutically acceptable carriers, excipients, or diluents. The composition can be prepared into different formulations, for example, a solid oral formulation, a liquid oral formulation, or an injection formulation. 
         [0042]    Said solid or liquid oral formulations include tablets, dispersible tablets, sugar-coated formulations, granules, dry powder, capsules and solution. 
         [0043]    For solid oral formulations, lactose or starch can be used as carrier; gelatin, methylcellulose or polyvinylpyrrolidone can be used as binder; starch, sodium carboxymethycellulose, or microcrystalline cellulose can be used as disintegrant; talc powder, silica gel colloid, stearin, calcium stearate or magnesium stearate can be used as anti-blocking agent or lubricant. 
         [0044]    The solid oral formulation can be prepared by mixing the active ingredient, a carrier and optionally a part of a disintegrant to yield a mixture; granulating the mixture with an aqueous solution, an alcoholic solution, or an aqueous alcoholic solution of the binder in an appropriate device; drying the resulting granules; and subsequently adding the remaining disintegrant, a lubricant and an anti-blocking agent to produce the formulation. 
         [0045]    The compounds of the invention can also be administered parenterally. Preferably, the parenteral administration is achieved by injection. 
         [0046]    The compounds of formula I have a broad range of effective dosage. For example, the dosage can be about 0. 1 mg/Kg to 500 mg/Kg of body weight per day. For an adult, the most preferable dosage is in the range between 1 mg/kg and 50 mg/kg of body weight, in one dose or several doses. The actual dosage of the compounds of formula I can be determined by doctors, depending on the conditions of the patient being treated, including physical condition, administration route, age, body weight, individual drug reaction, severity of symptoms and the like. 
         [0047]    The compounds of formula I were tested for their biological activities by the following methods. 
         [0048]    (1) Anti-Inflammatory Effect 
         [0049]    Male or female healthy ICR mice, each weighing 18-20 g, were grouped randomly into model, positive control and test groups based on body weight, with 8-10 animals for each group. The positive group was treated by hydrocortisone in a dose of 40 mg/kg of body weight. 
         [0050]    The compound of formula I to be tested was administered intragastrically at a concentration of 2 mg/ml in a 1% CMCNa solution, while a 1% CMCNa solution alone was administered to the model group in the same amount. 30 min after administration, 50 μl of xylene solution was added dropwise into the right ear of each mouse to cause an inflammation reaction. 30 min later, the mice were killed by breaking the cervical vertebrae. Both ears were excised immediately from the mices, and punched in the same position with a puncher having a diameter of 0.6 cm. The obtained ear piece was weighed. For each mouse, the weight ratio of swollen ear to normal ear was regarded as an indication for the degree of swelling. Biostatistical analysis was performed using the Student&#39;s t test. 
         [0051]    (2) Analgesic Effect 
         [0052]    Male or female healthy ICR mice, each weighing 18-22 g, were used. Each mouse was injected intraperitoneally with 0.6% acetic acid in a dose of 0.2 ml one day before the test. The mice with the writhing response occurring in the range of 10-50 times were selected. The selected mice were grouped randomly into model, positive control and test groups based on the occurrence of the writhing response, with 10 animals for each group. The positive group was treated with aspirin in a dose of 50 mg/kg of body weight. 
         [0053]    After fasting for at least 12 h, the animals were intragastrically administrated: the animals in model group are administered only with 1% CMCNa in the same amount. 1 h after administration, the mice were intraperitoneally injected with 0.6% acetic acid in a dose of 0.2 ml per animal. After 5 min, the occurrence of the writhing responses for one mouse was recorded during a period of 15 minutes. Based on the occurrence recorded, the inhibition rate of the active ingredient for writhing response was calculated. 
         [0000]      Inhibition rate=(means of the writhing occurrence for model group−means of the writhing occurrence for test group)/means of writhing occurrence for model group×100%. 
         [0054]    (3) Cough-Prevention 
         [0055]    Male or female healthy ICR mice, each weighing 18-22 g, were used. They were grouped randomly into model, positive control and test groups based on body weight, with 10 animals for each group. The positive group was treated by codeine phosphate in a dose of 50 mg/kg of body weight. 
         [0056]    After fasting for at least 12 h, the animals were intragastrically administrated: the animals in model group are administered only with 1% CMCNa in the same amount. To start the test, 1 h after administration, the mice were placed in a 500 ml container, into which a cotton ball sucking 0.3 ml of ammonia was added to cause coughing. The occurrence of cough was observed within 5 min. 
         [0000]      Inhibition rate of cough=(the cough occurrence for model group−the cough occurrence for test group)/the cough occurrence for model group×100%. 
         [0057]    (4) Aldosterone-Like Side Effects 
         [0058]    Three compounds of formula I of the invention were selected to examine their subacute toxicity within one month, with dexamethasone and glycyrrhetinic acid as control. All the animals died when administrated with dexamethasone in a dose of 300 mg/kg of body weight for one week, whereas no death was observed in the case of glycyrrhetinic acid and the compound of formula I of the invention under the same conditions. Furthermore, the level of aldosterone in blood plasma was determined. The level will decrease in a negative feedback way when an agent having aldosterone-like effects is administrated. The test confirmed the aldosterone-like effects of glycyrrhetinic acid, but such effects were not shown in the case of the compound of formula I of the invention. 
         [0059]    The results demonstrate that the compounds of formula I of the invention have high activities in anti-inflammation, analgesia, and cough-prevention. They also eliminate the aldosterone-like effects typical of glycyrrhetinic acid. The compounds of formula I of the invention exhibit improved safety and activities. 
         [0060]    In addition, results show that the compounds of formula I of the invention have the following effects: 
         [0061]    (1) For paracetamol/hepatitis mice models, GPT was decreased when DG6 was administrated in a dose of 100 mg/kg, which shows that the compound can protect liver. 
         [0062]    (2) For Influenza virus A-infected mice models, death rate caused by the virus infection was decreased when DG4 was administrated in a dose of 100 mg/kg. 
     
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0063]    The invention is described in detail by reference to the following examples. It is to be understood that the examples are intended to illustrate the invention and not to limit it. In light of the teaching of the invention, those skilled in the art can make various variations and modifications, which will be surely within the scope of claims of the application. 
         [0064]    Apparatus and Reagents 
         [0065]    BRUKER AV400 NMR spectrometer (CDCl 3  or DMSO-d 6  as internal standard). Glycyrrhetinic acid and other chemical reagents used are commercially available. 
       EXAMPLE 1  
     Preparation of 11-deoxyglycyrrhetinic acid 
       [0066]    
       
                 
         
             
             
         
       
     
         [0067]    The title compound was prepared by the process as described in CA, 1984, 100, 68568e. The crude product was recrystallized from acetic acid to obtain a colorless acicular crystal. Yield: 80.86%. m.p.: 329-331□. 
         [0068]      1 HNMR (400 MHz, CDCL 3 ) δ ppm: 0.66 (s, 3H), 0.67 (s, 3H), 0.85 (s, 6H), 0.88 (s, 3H), 0.93 (m, 2H), 1.01 (m, 3H), 1.05 (s, 3H), 1.35 (m, 5H), 1.55 (m, 8H), 1.85 (m, 8H), 3.01 (m, 1H), 5.15 (s, 1H).  13 CNMR (100 MHz, CDCL 3 ) δ ppm: 181.08 (—C═O), 145.23, 125.08, 68.25 (C 3 ), 64.59, 56.87, 47.38, 45.32, 45.22, 43.85, 43.52, 42.51, 39.82, 38.25, 36.25, 33.72, 32.83, 32.22, 28.79, 28.37, 27.97, 27.72, 25.30, 24.75, 19.89, 18.78, 16.87, 15.78, 15.41. 
       EXAMPLE 2  
     Preparation of 3-chloro-glycyrrhetinic acid chloride 
       [0069]    
       
                 
         
             
             
         
       
     
         [0070]    5 mmol of glycyrrhetinic acid was added to a dried erlenmeyer flask equipped with a magnetic stirrer, and then 50 ml of dichlorosulfoxide was added. The reaction mixture was stirred at room temperature and then at a slightly elevated temperature, until TLC showed no starting material remaining on TLC testing. The resulting mixture containing 3-chloro-glycyrrhetinic acid chloride was evaporated under reduced pressure to recover dichlorosulfoxide. The product was prepared immediately before use and introduced in the next step without purification. 
       EXAMPLE 3  
     Preparation of 3-chloro-11-deoxy glycyrrhetinic acid chloride 
       [0071]    
       
                 
         
             
             
         
       
     
         [0072]    3-chloro-11-deoxyglycyrrhetinic acid chloride was prepared as described in example 2 except using 11-deoxyglycyrrhetinic acid (the product of example 1) instead of glycyrrhetinic acid. It was prepared immediately before use and introduced in the next step without purification. 
       EXAMPLE 4  
     Preparation of 3-p-chlorophenyl-5-aminomethyl-isoxazole 
       [0073]    
       
                 
         
             
             
         
       
     
         [0074]    1.56 g (10 mmol) of p-chlorobenzaldoxime was dissolved in 40 ml of dried dichloromethane in a erlenmeyer flask equipped with a magnetic stirrer, and 1.7 g (12mmol) of N-chlorosuccinimide was added slowly. The reaction mixture was stirred until completely dissolved. The system was heated slightly for 20 min. Then, 0.56 g (10 mmol) of propargyl amine was introduced and 1.2 g (12mmol) of triethylamine was added dropwise, which caused the emission of white smog. The reaction mixture was heated under reflux for 2 h, and then purified by column chromatography on silica gel using petroleum ether (b.p. 60-90° C.)—ethyl acetate (v:v=4:1) as eluant, to obtain 2.3 g of the product as yellow solid. The yield was 62%. 
         [0075]      1 H NMR (CDCl 3 ), δ (ppm): 2.8 (s), 1H; 4.8 (s), 2H; 6.5 (s), 1H; 7.2-7.8 (m) 
       EXAMPLES 5-21  
       [0076]    The compounds of formula IIId shown below were prepared as described in example 4 except using different compounds of formula IIIa instead of p-chlorobenzaldoxime in each case. 
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                   
               
               
                 Example 
                   
                   
               
               
                 No. 
                 Compound IIIa 
                 Compound IIId 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 5 
                 o-chlorobenzaldoxime 
                 3-o-chlorophenyl-5-aminomethyl-isoxazole 
               
               
                 6 
                 o-methoxybenzaldoxime 
                 3-o-methoxyphenyl-5-aminomethyl-isoxazole 
               
               
                 7 
                 m-chlorobenzaldoxime 
                 3-m-chlorophenyl-5-aminomethyl-isoxazole 
               
               
                 8 
                 acetaldoxime 
                 3-methyl-5-aminomethyl-isoxazole 
               
               
                 9 
                 p-acetyl benzaldoxime 
                 3-p-acetylphenyl-5-aminomethyl-isoxazole 
               
               
                 10 
                 p-nitrobenzaldoxime 
                 3-p-nitrophenyl-5-aminomethyl-isoxazole 
               
               
                 11 
                 p-trifluoromethylbenzaldoxime 
                 3-p-trifluoromethylphenyl-5-aminomethyl-isoxazole 
               
               
                 12 
                 p-hydroxylbenzaldoxime 
                 3-p-hydroxylphenyl-5-aminomethyl-isoxazole 
               
               
                 13 
                 p-chlorobenzaldoxime 
                 3-p-chlorophenyl-5-aminomethyl-isoxazole 
               
               
                 14 
                 p-methoxybenzaldoxime 
                 3-p-methoxyphenyl-5-aminomethyl-isoxazole 
               
               
                 15 
                 p-methylbenzaldoxime 
                 3-p-methylphenyl-5-aminomethyl-isoxazole 
               
               
                 16 
                 p-fluorobenzaldoxime 
                 3-p-fluorophenyl-5-aminomethyl-isoxazole 
               
               
                 17 
                 4-pyridyl-formaldoxime 
                 3-(4-pyridin)yl-5-aminomethyl-isoxazole 
               
               
                 18 
                 benzaldoxime 
                 3-phenyl-5-aminomethyl-isoxazole 
               
               
                 19 
                 4-chloro-5-formylimidazole 
                 3-(4-chloroimidazol-5-yl)-5-aminomethyl-isoxazole 
               
               
                   
                 aldoxime 
               
               
                 20 
                 2,4-dichlorobenzaldoxime 
                 3-(2,4-dichlorophenyl)-5-aminomethyl-isoxazole 
               
               
                 21 
                 2,4-dimethoxybenzaldoxime 
                 3-(2,4-dimethoxyphenyl)-5-aminomethyl-isoxazole 
               
               
                   
               
             
          
         
       
     
       EXAMPLE 22  
     Preparation of N-[(3-p-methylphenyl-isoxazol-5-yl)methyl]-11-deoxyglycyrrhetinamide (DG2 ) 
       [0077]    
       
                 
         
             
             
         
       
     
         [0078]    0.5 mmol of 11-deoxyglycyrrhetinic acid (the product of example 1) and 0.55 mmol of 1-hydroxybenzotrizole (HOBt) were dissolved in a mixed solution of 8 ml of dichloromethane and 2 ml of DMF. The mixture was stirred at room temperature for 10 min and then in an ice bath. A solution of 0.55 mmol of N,N′-dicyclohexylcarbodiimide (DCC) in 6 ml of dichloromethane was added dropwise to the system above. The system was stirred in an ice bath for 30 min. Then a solution of 0.75 mmol of 3-p-methylphenyl-5-aminomethyl-isoxazole (the product of example 15) in 6 ml of dichloromethane was added dropwise to the system. After being stirred in an ice bath for 2 h, the system was allowed to warm up to room temperature. The reaction continued until TLC showed the termination of the reaction. At the end of the reaction, the precipitated solid (DCU) was filtered off. The filtrate was concentrated to dryness, and the resulting crude product was taken up in a small amount of the solvent. Column chromatography using a gradient elution (ethyl acetate:petroleum ether (60-90         ) 1:5-1:2, V/V) yielded N-[(3-p-methylphenyl-isoxazol-5-yl)methyl]-11-deoxyglycyrrhetinamide as white powder. m.p: 248-250          Yield: 33.02%. 
         [0079]      1 H-NMR (400 MHz DMSO-d 6 ) δ ppm: 0.68 (6H, d), 0.86-0.89 (1H, m), 1.04-1.11 (6H, d), 1.29 (6H, m), 1.38 (6H, m), 1.60-1.95 (8H, m), 1.98 (1H, t, 18β-H), 2.35 (3H, s, Ar—CH3). 2.00 (1H, dt, C3-H),4.30-4.46 (2H, m), 5.18 (1H, s,          12-H), 6.66 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]      7 . 30 -7.70 (4H, m, Ar—H), 8.24 (1H, brs, —NH—). 
       EXAMPLES 23-47  
       [0080]    The compounds of formula Ia shown below were prepared as described in example 22, except using different compounds of formula IIId instead of 3-p-methylphenyl-5-aminomethyl-isoxazole of example 4 in the reaction with compound IIa or IIb. 
         [0000]    
       
         
               
               
               
               
               
               
             
           
               
                   
               
               
                 Example 
                   
                   
                   
                   
                   
               
               
                 No. 
                 IIId 
                 IIa, IIb 
                 Compound Ia 
                 No. 
                 m.p. □ 
               
               
                   
               
             
             
               
                 23 
                 3-p-hydroxylphenyl- 
                 glycyrrhetinic acid 
                 N-[(3-p-hydroxylphenyl-isoxazol-5-yl) 
                 G1 
                 208-210 
               
               
                   
                 5-aminomethyl-isoxazole 
                   
                 methyl]-glycyrrhetinamide 
               
               
                 24 
                 3-p-methylphenyl-5- 
                 glycyrrhetinic acid 
                 N-[(3-p-methylphenyl-isoxazol-5-yl) 
                 G2 
                 249-250 
               
               
                   
                 aminomethyl-isoxazole 
                   
                 methyl]-glycyrrhetinamide 
               
               
                 25 
                 3-p-fluorophenyl-5- 
                 glycyrrhetinic acid 
                 N-[(3-p-fluorophenyl-isoxazol-5-yl) 
                 G3 
                 243-244 
               
               
                   
                 aminomethyl-isoxazole 
                   
                 methyl]-glycyrrhetinamide 
               
               
                 26 
                 3-o-chlorophenyl-5- 
                 glycyrrhetinic acid 
                 N-[(3-o-chlorophenyl-isoxazol-5-yl) 
                 G4 
                 214-217 
               
               
                   
                 aminomethyl-isoxazole 
                   
                 methyl]-glycyrrhetinamide 
               
               
                 27 
                 3-p-methoxyphenyl- 
                 glycyrrhetinic acid 
                 N-[(3-p-methoxyphenyl-isoxazol-5-yl) 
                 G5 
                 247-249 
               
               
                   
                 5-aminomethyl-isoxazole 
                   
                 methyl]-glycyrrhetinamide 
               
               
                 28 
                 3-o-methoxyphenyl- 
                 glycyrrhetinic acid 
                 N-[(3-o-methoxyphenyl-isoxazol-5-yl) 
                 G6 
                 278-280 
               
               
                   
                 5-aminomethyl-isoxazole 
                   
                 methyl]-glycyrrhetinamide 
               
               
                 29 
                 3-methyl-5-aminomethyl- 
                 glycyrrhetinic acid 
                 N-[(3-methyl-isoxazol-5-yl)methyl]- 
                 G7 
                 156-159 
               
               
                   
                 isoxazole 
                   
                 glycyrrhetinamide 
               
               
                 30 
                 3-p-chlorophenyl-5- 
                 18-α, glycyrrhetinic 
                 18-α, 
                 G8 
                 203-205 
               
               
                   
                 aminomethyl-isoxazole 
                 acid 
                 N-[(3-p-chlorophenyl-isoxazol-5-yl) 
               
               
                   
                   
                   
                 methyl]-glycyrrhetinamide 
               
               
                 31 
                 3-p-trifluoromethyl 
                 glycyrrhetinic acid 
                 N-[(3-p-trifluoromethylphenyl-isoxazol- 
                 G9 
                 175-176 
               
               
                   
                 phenyl-5-aminomethyl- 
                   
                 5-yl)methyl]-glycyrrhetinamide 
               
               
                   
                 isoxazole 
               
               
                 32 
                 3-phenyl-5-aminomethyl- 
                 glycyrrhetinic acid 
                 N-[(3-phenyl-isoxazol-5-yl)methyl]- 
                 G10 
                 214-216 
               
               
                   
                 isoxazole 
                   
                 glycyrrhetinamide 
               
               
                 33 
                 3-p-hydroxylphenyl- 
                 11-deoxy- 
                 N-[(3-p-hydroxylphenyl-isoxazol-5-yl) 
                 DG1 
                 214-218 
               
               
                   
                 5-aminomethyl-isoxazole 
                 glycyrrhetinic acid 
                 methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 34 
                 3-p-methylphenyl-5- 
                 11-deoxy- 
                 N-[(3-p-methylphenyl-isoxazol-5-yl) 
                 DG2 
                 248-250 
               
               
                   
                 aminomethyl-isoxazole 
                 glycyrrhetinic acid 
                 methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 35 
                 3-p-fluorophenyl-5- 
                 11-deoxy- 
                 N-[(3-p-fluorophenyl-isoxazol-5-yl) 
                 DG3 
                 254-256 
               
               
                   
                 aminomethyl-isoxazole 
                 glycyrrhetinic acid 
                 methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 36 
                 3-o-chlorophenyl-5- 
                 11-deoxy- 
                 N-[(3-o-chlorophenyl-isoxazol-5-yl) 
                 DG4 
                 245-248 
               
               
                   
                 aminomethyl-isoxazole 
                 glycyrrhetinic acid 
                 methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 37 
                 3-o-methoxyphenyl- 
                 11-deoxy- 
                 N-[(3-o-methoxyphenyl-isoxazol-5-yl) 
                 DG6 
                 266-268 
               
               
                   
                 5-aminomethyl-isoxazole 
                 glycyrrhetinic acid 
                 methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 38 
                 3-methyl-5-aminomethyl- 
                 11-deoxy- 
                 N-[(3-methyl-isoxazol-5-yl)methyl]- 
                 DG7 
                 209-211 
               
               
                   
                 isoxazole 
                 glycyrrhetinic acid 
                 11-deoxy-glycyrrhetinamide 
               
               
                 39 
                 3-m-chlorophenyl-5- 
                 11-deoxy- 
                 N-[(3-m-chlorophenyl-isoxazol-5-yl) 
                 DG8 
                 215-218 
               
               
                   
                 aminomethyl-isoxazole 
                 glycyrrhetinic acid 
                 methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 40 
                 3-p-acetylphenyl-5- 
                 11-deoxy- 
                 N-[(3-p-acetylphenyl-isoxazol-5-yl) 
                 DG9 
                 231-235 
               
               
                   
                 aminomethyl-isoxazole 
                 glycyrrhetinic acid 
                 methyl]-11-deoxy-glycyrrhetinamide 
               
               
                 41 
                 3-p-nitrophenyl-5-aminomethyl- 
                 18-α,11-deoxy- 
                 18-α, 
                 DG10 
                 245-248 
               
               
                   
                 isoxazole 
                 glycyrrhetinic acid 
                 N-[(3-p-nitrophenyl-isoxazol-5-yl)methyl]- 
               
               
                   
                   
                   
                 11-deoxy-glycyrrhetinamide 
               
               
                 42 
                 3-(4-pyridin)yl-5-aminomethyl- 
                 11-deoxy- 
                 N-[(3-(4-pyridin)yl-isoxazol-5-yl)methyl]- 
                 DG11 
                 245-247 
               
               
                   
                 isoxazole 
                 glycyrrhetinic acid 
                 11-deoxy-glycyrrhetinamide 
               
               
                 43 
                 3-(4-chloroimidazol- 
                 11-deoxy- 
                 N-{[3-(4-chloroimidazol)-5-yl-isoxazol- 
                 DG12 
                 210-212 
               
               
                   
                 5-yl)- 
                 glycyrrhetinic acid 
                 5-yl]methyl}-11-deoxy-glycyrrhetinamide 
               
               
                   
                 5-aminomethyl-isoxazole 
               
               
                 44 
                 3-(2,4-dichlorophenyl)- 
                 11-deoxy- 
                 N-{[3-(2,4-dichlorophenyl)-isoxazol- 
                 DG13 
                 232-235 
               
               
                   
                 5-aminomethyl- 
                 glycyrrhetinic acid 
                 5-yl]methyl}-11-deoxy-glycyrrhetinamide 
               
               
                   
                 isoxazole 
               
               
                 45 
                 3-(2,4-dimethoxy 
                 11-deoxy- 
                 N-{[3-(2,4-dimethoxyphenyl)-isoxazol- 
                 DG14 
                 189-192 
               
               
                   
                 phenyl)-5-aminomethyl- 
                 glycyrrhetinic acid 
                 5-yl]methyl}-11-deoxy-glycyrrhetinamide 
               
               
                   
                 isoxazole 
               
               
                 46 
                 3-p-trifluoromethyl 
                 11-deoxy- 
                 N-[(3-p-trifluoromethylphenyl-isoxazol- 
                 DG15 
                 260-262 
               
               
                   
                 phenyl-5-aminomethyl- 
                 glycyrrhetinic acid 
                 5-yl)methyl]-11-deoxy-glycyrrhetinamide 
               
               
                   
                 isoxazole 
               
               
                 47 
                 3-phenyl-5-aminomethyl- 
                 11-deoxy- 
                 N-[(3-phenyl-isoxazol-5-yl)methyl]- 
                 DG16 
                 245-247 
               
               
                   
                 isoxazole 
                 glycyrrhetinic acid 
                 11-deoxy-glycyrrhetinamide 
               
               
                   
               
             
          
         
       
     
         [0081]    Some of the compounds in the above table were characterized by the following  1 H-NMR data. 
         [0082]    G2:  1 H-NMR (400 MHz, CDCl 3 , TMS) δ: 0.80-0.89 (6H, m), 1.00-1.10 (6H, m), 1.13-1.21 (12H, m), 1.31-1.48 (8H, m), 1.53-1.58 (2H, m), 1.68-2.03 (6H, m), 2.03-2.07 (1H, t, 18β-H), 2.33 (s, 1H, C 9 —H), 2.39 (s, 3H, Ar—CH 3 ), 2.78-2.81 (1H, d), 3.20-3.24 (t, 1H, C 3 —H), 4.59-4.66 (2H, m), 5.69 (s, 1H,            12 -H), 6.10 (1H, brs, —NH—), 6.44 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 24 -7.67 (4H, m, Ar—H). 
       [0083]    G3:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.69-0.71 (6H, m), 0.91 (6H, t), 1.03-1.24 (12H, m), 1.29-1.35 (8H, m), 1.50 (d, 2H), 1.66-1.92 (6H, m), 2.07-2.09 (1H, t, 18β-H), 2.32 (s, 1H, C 9 —H), 2.57-2.60 (d, 1H, —OH), 3.00-3.02 (m, 1H, C 3 —H), 4.45-4.50 (2H, m), 5.51 (s, 1H,            12 -H), 6.75 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 33  (t, 2H, Ar—H), 7.89 (t, 2H, Ar—H), 8.31 (t, 1H, —NH—). 
       [0084]    G5:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.69-0.71 (d, 6H,), 0.91 (6H, m), 1.03-1.12 (12H, m), 1.29-1.35 (8H, m), 1.50 (2H, d), 1.66-1.91 (6H, m), 2.09 (t, 1H, 18β-H), 2.32 (s, 1H, C 9 —H), 2.57 (d, 1H, —OH), 3.00 (dt, 1H, C3-H), 3.81 (s, 3H, —OCH3), 4.44 (m, 2H), 5.52 (s, 1H,            2 -H), 5.67 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 03 -7.05 (d, 2H, Ar—H), 7.75-7.77 (d, 2H, Ar—H), 8.29 (t, 1H, —NH—). 
       [0085]    G6:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.68-0.71 (d, 6H), 0.90-0.92 (6H, m), 1.11-1.16 (12H, m), 1.29-1.35 (8H, m), 1.49-1.52 (2H, d), 1.67-1.98 (6H, m), 2.06-2.09 (t, 1H, 18β-H), 2.57-2.61 (d, 1H), 2.99-3.02 (m, 1H, C 3 —H), 3.81 (s, 3H, —OCH 3 ), 4.43-4.48 (m, 2H), 5.51 (s, 1H,            2 -H), 5.58 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 01 -7.73 (m, 4H, Ar—H), 8.29-8.31 (t, 1H, Hz,—NH—). 
       [0086]    G9:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.70-0.71 ( 6H, d ), 0.77-0.81 (1H, m), 0.82-0.84 (5H, m), 0.85-0.93 (6H, d), 1.02-1.09 (6H, d), 1.31-1.35 (8H, m), 1.51-1.66 (6H, m) 2.08 (2H, m), 2.31 (1H, s), 2.51-2.52 (1H, d), 3.00-3.03 (1H, m), 4.31 (1H, s), 4.49-4.54 (2H, m) 5.53 (s, 1H,           2 -H), 6.89 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 86 -7.88 (2H, d, Ar—H), 8.07-8.09 (2H, d, Ar—H), 8.33-8.36 (1H, t, NH). 
       [0087]    G10:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.69-0.71 (d, 6H), 0.91-0.97 (m, 6H), 1.03-1.08 (m, 12H), 1.30-1.35 (m, 8H), 1.50 (m, 2H), 1.66-1.92 (m, 6H), 2.07-2.09 (m, 1H, 18β-H), ,2.31 (s, 1H, C9-H), 2.57-2.60 (d, 1H, —OH), 3.01-3.02 (m, 1H, C 3 —H), 4.45-4.50 (m, 2H), 5.52 (s, 1H,            12 -H), 6.74 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 49 -7.83 (m, 5H, Ar), 8.31 (t, 1H, —NH—). 
       [0088]    DG2:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.68 (6H, d), 0.86-0.89 (1H, m), 1.04-1.11 (6H, d), 1.29 (6H, m), 1.38 (6H, m), 1.60-1.95 (8H, m), 1.98 (1H, t, 18β-H), 2.35 (3H, s, Ar—CH 3 ), 2.99 (1H, dt, C 3 —H), 4.30-4.46 (2H, m), 5.18 (1H, s,            12 -H), 6.66 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]      7 . 30 -7.70 (4H, m, Ar—H), 8.24 (1H, brs, —NH—). 
         [0089]    DG3:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.68 (6H, d), 0.81-0.89 (m, 12H), 1.04 (s, 3H), 1.11 (d, 3H), 1.19-1.24 (6H, m), 1.29-1.35 (6H, m), 1.45-1.52 (8H, m), 1.99 (t, 1H, 18β-H), 2.99 (dt, 1H, C 3 —H), 4.41-4.47 (2H, m), 5.18 (s, 1H,           12 -H), 6.74 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 32 -7.90 (4H, m, Ar—H), 8.24 (t, 1H, —NH—). 
       [0090]    DG4:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.67-0.69 (6H, d), 0.81-0.92 (12H, m), 1.05 (3H, s), 1.11 (3H, s), 1.29-1.52 (6H, m), 1.79-1.87 (7H, m), 2.09 (1H, s), 2.99-3.01 (1H, m), 4.29-4.30 (1H, m), 4.42-4.48 (2H, m), 5.18 (1H, s,            12 -H), 6.76 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 56 -7.58 (2H,  d, Ar—H), 7.85-7.87 (2H, d, Ar—H), 8.22-8.25 (1H, t, —NH—). 
       [0091]    DG6:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.68-0.70 (6H, d), 0.85-0.91 (12H, m), 1.64-1.11 (6H, m), 1.90 (1H, t, 18β-H), 1.23-1.29 (6H, m), 1.44-1.52 (6H, m), 1.60-1.81 (8H, m), 2.98-3.00 (1H, dt, C 3 —H), 4.39-4.46 (2H, m), 5.20 (1H, s,            12 -H), 6.56 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 01 -7.73 (4H, m, Ar—H), 8.21-8.24 (1H, t, —NH—). 
       [0092]    DG15:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.69-0.74 (6H, d), 0.77-0.95 (12H, m), 1.06-1.11 (6H, d), 1.27-1.52 (6H, m), 1.77-1.79 (6H, m), 1.84-2.51 (8H, m), 3.01 (1H, m), 4.29-4.30 (1H, m) 4.46-4.47 (2H, m), 5.18 (1H, s,            12 -H), 6.86 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 86 -7.88 (2H, d, Ar—H), 8.06-8.08 (2H, d, Ar—H), 8.26 (1H, t, —NH—). 
       [0093]    DG16:  1 H-NMR (400 MHz, DMSO-d 6 , TMS) δ: 0.67-0.70 (d, 6H), 0.85-0.96 (m, 12H), 1.05 (s, 3H), 1.12 (s, 3H), 1.23-1.35 (m, 6H), 1.45-1.53 (m, 6H), 1.80-1.88 (m, 7H), 1.99 (m, 1H), 3.00 (brs, 1H), 4.30 (s, 1H), 4.42-4.49 (m, 2H), 5.19 (s, 1H,            12 -H), 6.72 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 49 -7.51 (t, 3H, Ar—H), 7.81-7.83 (t, 2H, Ar—H), 8.24 (t, 1H, —NH—). 
     EXAMPLE 48  
     Preparation of N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-chloro-11-deoxyglycyrrhetinamide  (DG5) 
       [0094]    
       
                 
         
             
             
         
       
     
         [0095]    0.5 mmol of 3-chloro-11-deoxyglycyrrhetinic acid (the product of example 3) was dissolved in a mixed solution of 8 ml of dichloromethane and 2 ml of DMF and stirred in an ice bath for 30 min. Then, a solution of 0.5 mmol of 3-p-methoxyphenyl-5-aminomethyl-isoxazole (the product of example 14) in 6 ml of dichloromethane was added dropwise to the system above. 0.5 mmol of potassium carbonate was added in several portions. After being stirred in an ice bath for 2 h, the system was allowed to warm up to the room temperature. The reaction continued until TLC showed the termination of the reaction. Afterwards the precipitated solid was filtered off. The filtrate was concentrated to dryness, and the residue was taken up in a small amount of the solvent. Column chromatography using a gradient elution (ethyl acetate:petroleum ether (60-90         ) 1:5-1:2, V/V) yielded N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-chloro-11-deoxyglycyrrhetinamide as white powder. m.p.:240-245         . 
         [0096]      1 H-NMR (400 MHz, DMSO-d 6 ) δ ppm: 0.68 (6H, d), 0.86-0.89 (1H, m), 1.06-1.14 (6H, d), 131 (6H, m), 1.39 (6H, m), 1.62-1.98 (8H, m), 1.99 (1H, t, 18β-H), 2.36 (3H, s, Ar—CH 3 ), 3.27 (1H, dt, C 3 —H), 4.32-4.48 (2H, m), 5.20 (1H, s,            2 -H), 6.64 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]      7 . 32 -7.72 (4H, m, Ar—H), 8.26 (1H, brs, —NH—). 
       EXAMPLE 49  
     Preparation of N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]-3-acetoxy-glycyrrhetinamide (RG1) 
       [0097]    20 ml of tetrahydrofuran was added to 0.5 mmol of N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]-glycyrrhetinamide (the product of example 28) and then an aqueous solution of 1 mmol of potassium carbonate was added. The system was cooled to 0         . Acetyl chloride (1.2 mol) was added dropwise at 0° C., and the reaction was continued until TLC showed no starting material remaining. After the addition of water, the system was extracted with dichloromethane. The removing of the solvent yielded N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]-3-acetoxy-glycyrrhetinamide. 
         [0098]      1 H-NMR (400 MHz DMSO-d 6 ) δ ppm: 0.68-0.71 (d, 6H,), 0.90-0.92 (6H, m), 1.11-1.16 (12H, m), 1.29-1.35 (8H, m), 1.49-1.52 (2H, d), 1.67-1.98 (6H, m), 2.01-2.09 (t, 4H, 18β-H), 2.57-2.61 (d, 1H), 2.99-3.02 (m, 1H, C 3 —H), 3.82 (s, 3H, —OCH 3 ), 4.43-4.49 (m, 2H), 5.53 (s, 1H,            2 -H), 5.59 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 03 -7.76 (m, 4H, Ar—H), 8.29-8.31 (t, 1H, Hz, —NH—). 
     EXAMPLE 50  
     Preparation of N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]-3-carboxymethoxy-glycyrrhetinamide  (RG2) 
       [0099]    20 ml of tetrahydrofuran was added to 1 mmol of N-[(3-o-methoxyphenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide (the product of example 28), and an aqueous solution of 1 mol of potassium carbonate was added. The system was cooled to 0° C. Ethyl bromoacetate (1.2 mol) was added dropwise at 0° C., and the reaction was continued at room temperature until TLC showed no starting material remaining. After the addition of water and then an aqueous solution of 1 mol of potassium carbonate, the reaction was stirred at a slightly elevated temperature. After the end of deesterification, the solution was acidified and extracted with dichloromethane. The removing of the solvent yielded N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]-3-carboxymethoxy-glycyrrhetinamide. 
         [0100]      1 H-NMR (400 MHz, DMSO-d 6 ) δ ppm: 0.68-0.71 (d, 6H,), 0.90-0.92 (6H, m), 1.11-1.16 (12H, m), 1.29-1.35 (8H, m), 1.49-1.52 (2H, d), 1.67-1.98 (6H, m), 2.06-2.09 (t, 1H, 18β-H), 2.57-2.61 (d, 1H), 2.99-3.02 (m, 1H, C 3 —H), 3.81 (s, 3H, —OCH 3 ), 4.01 (s, 2H, —COCH 3 O—), 4.46-4.49 (m, 2H), 5.54 (s, 1H,            12 -H), 5.57 
         [0000]    
       
                 
         
             
             
         
       
     
         7 . 11 -7.83 (m, 4H, Ar—H), 8.39-8.41 (t, 1H, Hz, —NH—). 
     EXAMPLE 51 
     Preparation of N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-amino-11-deoxy glycyrrhetinamide (ADG1) 
       [0101]    0.5 mmol of N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-choloro-11-deoxy glycyrrhetinamide (the product of example 48) was dissolved in 8 ml of dichloromethane. Ammonia gas was introduced with stirring at room temperature, and if necessary, at a slightly elevated temperature. The reaction mixture was stirred until TLC showed the termination of the reaction. Afterwards, the precipitated solid was filtered off. The filtrate was concentrated to dryness, and the residue was recrystallized from a mixed solution of ethanol and water to yield N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-amino-I 1-deoxyglycyrrhetinamide. 
         [0102]      1 H-NMR (400 MHz, DMSO-d 6 ) δ ppm: 0.68 (6H, d), 0.86-0.89 (1H, m), 1.06-1.14 (6H, d), 1.31 (6H, m), 1.39 (6H, m), 1.66-1.99 (8H, m), 1.99 (1H, t, 18β-H), 2.36 (3H, s, Ar—CH3), 2.67 (1H, dt, C3-H), 4.32-4.48 (2H, m), 5.20 (1H, s,          12-H), 6.64 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]      7 . 32 -7.72 (4H, m, Ar—H), 8.26 (1H, brs, —NH—). 
       EXAMPLE 52  
     Preparation of N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-diethylamino-11-deoxyglycyrrhetinamide  (ADG2 ) 
       [0103]    0.5 mmol of the product of example 51 was dissolved in 10 ml of dichloromethane. 1.2 mmol of diethylamine was added. Then the reaction mixture was stirred at room temperature and a solution of 1.2 mmol of ethyl bromide in dichloromethane was added dropwise. The reaction mixture was stirred until TLC showed the termination of the reaction. Afterwards the precipitated solid was filtered off. The filtrate was concentrated to dryness and the residue was recrystallized from a mixed solution of ethanol and water to yield N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-diethylamino-11-deoxyglycyrrhetinamide. 
         [0104]      1 H-NMR (400 MHz, DMSO-d 6 ) δ ppm: 0.68 (6H, d), 0.86-0.89 (1H, m), 1.01-1.18 (12H), 1.32 (6H, m), 1.39 (6H, m), 1.66-1.99 (8H, m), 2.01 (1H, t, 18β-H), 2.33 (3H, s, Ar—CH 3 ), 2.35-2.65 (5H, C 3 —H and the methylene in diethylamino group ), 4.32-4.48 (2H, m), 5.21 (1H, s,            12 -H), 6.63 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]      7 . 32 -7.75 (4H, m, Ar—H), 8.27 (1H, brs, —NH—) 
       EXAMPLE 53  
     Preparation of N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-acetylamino-11-deoxyglycyrrhetinamide  (ADG3) 
       [0105]    0.5 mmol of the product of example 51 was dissolved in 10 ml of dichloromethane. 0.6 mmol of triethylamine was added. Then the reaction mixture was stirred at room temperature and 0.6 mmol of acetyl chloride was added dropwise. The reaction mixture was stirred until TLC showed the termination of the reaction. Afterwards, the resultant liquid was concentrated to dryness, and the residue was recrystallized from a mixed solution of ethanol and water to yield N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-acetylamino-11-deoxyglycyrrhetinamide. 
         [0106]      1 H-NMR (400 MHz, DMSO-d 6 ) δ ppm: 0.69 (6H, d), 0.87-0.91 (1H, m), 1.03-1.21 (6H), 1.33 (6H, m), 1.39 (6H, m), 1.66-1.99 (8H, m), 1.99-2.03 (4H, 18β-H and the hydrogen in acetylamino group), 2.36 (3H, s, Ar—CH 3 ), 2.33-2.41 (1H, C 3 —H), 4.32-4.48 (2H, m) 5.22 (1H, s,            2 -H), 6.66 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]      7 . 34 -7.74 (4H, m, Ar—H), 8.28 (1H, brs, —NH—). 
       EXAMPLE 54  
     Preparation of N-[(3-o-chlorophenyl-isoxazol-5-yl)methyl]-3-ethoxy-glycyrrhetinamide (RG3) 
       [0107]    20 ml of tetrahydrofuran was added to 1 mmol of N-[(3-o-chlorophenyl-isoxazol-5-yl) methyl]-glycyrrhetinamide (the product of example 26), and then an aqueous solution of 1 mol of potassium carbonate was added. The system was cooled to 0         . Ethyl bromide (1.2 mol) was added dropwise at 0         . After the addition, the reaction was continued at room temperature until TLC showed no starting material remaining. The reaction mixture was extracted with dichloromethane, followed by the recovery of the solvent to yield N-[(3-o-chlorophenyl-isoxazol-5-yl)methyl]-3-ethoxy-glycyrrhetinamide. Yield: 35%. m.p.: 198-201         . 
       EXAMPLE 55 
     Preparation of Sodium {N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]glycyrrhetinamide-3-oxy}acetate (YRG1) 
       [0108]    5 ml of an aqueous solution containing 0.55 mmol of NaOH was added to 0.5 mmol of N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]-3-carboxymethoxy-glycyrrhetinamide (the product of example 50). The mixture was stirred at a slightly elevated temperature until dissolution. An appropriate amount of ethanol was added, and the system was allowed to stand at 0          for crystallization. The resulting crystal was filtered out and dried to yield Sodium {N-[(3-o-methoxyphenyl-isoxazol-5-yl)methyl]glycyrrhetinamide-3-oxy}acetate Yield: about 60%. 
       EXAMPLE 56  
     Preparation of the salt of triethylammonium {N-[(3-o-methoxyphenyl-isoxazol-5-yl)-methyl]glycyrrhetinamide-3-oxy}acetate (YADG3) 
       [0109]    10 ml of dichloromethane was added to 0.5 mmol of N-[(3-o-methoxyphenyl-isoxazol-5-yl) methyl]-3-carboxymethoxy-glycyrrhetinamide (the product of example 50), followed by the addition of 0.55 mmol of triethylamine. The reaction mixture was stirred under reflux for 1 h and allowed to cool to room temperature for crystallization. The resulting crystal was filtered out, and dried to yield the salt of triethylammonium {N-[(3-o-methoxyphenyl-isoxazol-5-yl)-methyl]glycyrrhetinamide-3-oxy}acetate. Yield: about 5 0%. 
       EXAMPLE 57  
     Preparation of N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-ammonium-11-deoxyglycyrrhetinamide  hydrochloride (YADG1) 
       [0110]    10 ml of a 5% aqueous solution of HCl was added to 0.5 mmol of N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-amino-11-deoxyglycyrrhetinamide (the product of example 51). The mixture was stirred at a slightly elevated temperature to give a solution. An appropriate amount of ethanol was added, and the system was allowed to stand at 0          for crystallization. The resulting crystal was filtered out, and dried to yield N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-ammonium-11-deoxyglycyrrhetinamide hydrochloride. Yield: about 65%. 
       EXAMPLE 58  
     Preparation of N-[(3-p-methoxyphenyl-isoxazol-5-yl)methyl]-3-ammonium-11-deoxyglycyrrhetinamide  acetate (YADG2) 
       [0111]    10 ml of dichloromethane was added to 0.5 mmol of N-[(3-p-methoxyphenyl-isoxazol-5-yl) methyl]-3-amino-11-deoxyglycyrrhetinamide (the product of example 51), followed by the addition of 2 ml of acetic acid. The system was stirred under reflux for 1 h and then allowed to cool to the room temperature for crystallization. The resulting crystal was filtered out, and dried to yield N-[(3-p-methxoyphenyl-isoxazol-5-yl)methyl]-3-ammonium-11-deoxyglycyrrhetinamide acetate. Yield: about 60%. 
       PHARMACEUTICAL PREPARATION EXAMPLES  
     Example 1  
       [0112]    Preparation of a tablet comprising the compound of the invention as active ingredient in an amount of 100 mg per tablet: 
         [0000]    
       
         
               
               
             
               
               
               
             
           
               
                   
                   
               
               
                   
                 Amount per tablet 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Test sample G4 
                 100 mg  
               
               
                   
                 Microcrystalline cellulose 
                 55 mg  
               
               
                   
                 Starch 
                 45 mg  
               
               
                   
                 Hydroxymethylcellulose 
                 4 mg 
               
               
                   
                 Sodium carboxymethyl starch 
                 5 mg 
               
               
                   
                 Magnesium stearate 
                 1 mg 
               
               
                   
                 Talc powder 
                 1 mg 
               
               
                   
                   
               
             
          
         
       
     
         [0113]    All the ingredients were used in the above-mentioned amounts. The active ingredient, starch and cellulose were sieved and mixed sufficiently. The mixture powder was mixed with an aqueous hydroxymethylcellulose solution and then sieved to obtain wet granules. The granules were dried at 50-60° C. The pre-sieved sodium carboxymethyl starch, magnesium stearate and talc powder were added to the granules and then pressed to obtain tablets. 
       Example 2 
       [0114]      
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
             
               
               
               
               
             
           
               
                   
               
               
                 Preparation of injection 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 Test RG2 
                 100 
                 mg 
               
               
                   
                 Sodium citrate 
                 50 
                 mg 
               
               
                   
                 PEG3000 
                 10 
                 mg 
               
             
          
           
               
                   
                 Sodium hydroxide 
                 an appropriate amount 
               
             
          
           
               
                   
                 Distilled water 
                 10 
                 ml 
               
               
                   
                   
               
             
          
         
       
     
         [0115]    The mixture was adjusted to pH 7.5-8.5, and then filtered. The filtrate at a concentration of 1 mg/ml was divided into aliquots of 2 ml per ampoule and then sterilized to yield injections. 
         [0116]    The activity data of the compounds of formula I of the invention are shown below. 
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                   
               
               
                 Data of Anti-inflammatory Activity 
               
             
          
           
               
                   
                   
                   
                 Swelling degree of the swollen 
               
               
                   
                 Group 
                 Dose 
                 ear model (%) (  x  ± sd) 
               
               
                   
                   
               
               
                   
                 model 
                 — 
                 116.1 ± 33.4   
               
               
                   
                 hydrocortisone 
                 40 mg/kg 
                 55.7 ± 35.4** 
               
               
                   
                 G2 
                 40 mg/kg 
                 68.6 ± 29.5** 
               
               
                   
                 G3 
                 40 mg/kg 
                 60.8 ± 27.9** 
               
               
                   
                 G6 
                 40 mg/kg 
                 53.4 ± 35.2** 
               
               
                   
                 DG2 
                 40 mg/kg 
                 80.2 ± 18.8*  
               
               
                   
                 DG3 
                 40 mg/kg 
                 56.1 ± 28.3** 
               
               
                   
                 DG6 
                 40 mg/kg 
                 64.4 ± 29.2** 
               
               
                   
                   
               
               
                   
                 n: 8-10; 
               
               
                   
                 Note: 
               
               
                   
                 *p &lt; 0.05, 
               
               
                   
                 **p &lt; 0.01 compared to model group 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                   
               
               
                 Data of Analgesic Activity 
               
             
          
           
               
                   
                   
                 Writhing occurrence 
                 Inhibition rate of 
               
               
                 Group 
                 Dose (mg/kg) 
                 (  x  ± s) 
                 writhing response (%) 
               
               
                   
               
               
                 model 
                 50 
                 20.33 ± 17.52 
                 — 
               
               
                 G1 
                 50 
                 16.33 ± 10.57 
                 19.7 
               
               
                 DG4 
                 50 
                  6.56 ± 6.19* 
                 67.8 
               
               
                 DG6 
                 50 
                  8.00 ± 6.48* 
                 60.7 
               
               
                 DG3 
                 50 
                 18.33 ± 13.56 
                 9.8 
               
               
                 DG7 
                 50 
                 15.78 ± 12.43 
                 22.4 
               
               
                 DG8 
                 50 
                  15.89 ± 16.67* 
                 21.9 
               
               
                 aspirin 
                 50 
                  5.44 ± 5.87* 
                 73.2 
               
               
                   
               
               
                 n = 10; 
               
               
                 *p &lt; 0.05, 
               
               
                 **p &lt; 0.01, compared to model group 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                   
               
               
                 Data of Cough-preventing Activity 
               
             
          
           
               
                   
                   
                 Dose 
                 Cough occurrence 
                 Inhibition rate of 
               
               
                   
                 Group 
                 (mg/kg) 
                 (  x  ± sd) 
                 cough (%) 
               
               
                   
                   
               
               
                   
                 model 
                 50 
                 27.80 ± 11.70 
                 — 
               
               
                   
                 G1 
                 50 
                 28.20 ± 15.39 
                 −1.44 
               
               
                   
                 DG4 
                 50 
                 18.80 ± 7.04* 
                 32.37 
               
               
                   
                 DG6 
                 50 
                 22.60 ± 9.56  
                 18.71 
               
               
                   
                 DG3 
                 50 
                 17.70 ± 7.10* 
                 36.33 
               
               
                   
                 DG7 
                 50 
                 20.40 ± 14.31 
                 26.62 
               
               
                   
                 DG8 
                 50 
                 17.90 ± 8.21* 
                 35.61 
               
               
                   
                 RG1 
                 50 
                 17.64 ± 6.34* 
                 36.55 
               
               
                   
                 codeine 
                 50 
                  8.40 ± 8.95** 
                 69.78 
               
               
                   
                 phosphate 
               
               
                   
                   
               
               
                   
                 n = 10; 
               
               
                   
                 *p &lt; 0.05, 
               
               
                   
                 **p &lt; 0.01, compared to model group 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
           
               
                   
               
               
                 Data of Aldosterone-like Side Effects 
               
             
          
           
               
                   
                   
                 Aldosterone level in blood plasma 
               
               
                 Group 
                 Dose (mg/kg) 
                 (ng/ml) (  x  ± sd) 
               
               
                   
               
               
                 blank 
                 — 
                 705.2 ± 464.9 
               
               
                 glycyrrhetinic 
                 300 mg/kg 
                  68.3 ± 12.5* 
               
               
                 acid 
               
               
                 DG3 
                 300 mg/kg 
                 971.8 ± 359.1 
               
               
                 DG8 
                 300 mg/kg 
                 890.4 ± 220.7 
               
               
                 DG4 
                 300 mg/kg 
                 987.8 ± 342.2 
               
               
                   
               
               
                 n = 8; 
               
               
                 *p &lt; 0.05 compared to model group