Abstract:
Benzothiazolone Derivatives represented by the formula: ##STR1## in which X is halogen, A is alkylene and R is alkoxy, amino, substituted amino, carboxyalkyl or carboxyalkylene, which are useful as antiallergic or antiinflammatory agents.

Description:
BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     This invention relates to novel benzothiazolinone derivatives, their production and pharmaceutical composition. The compounds of the invention are useful as anti-allergic or anti-inflammatory agents. 
     2. Description of the Prior Art 
     Certain 3-substituted-carbonyl(lower)alkyl-2-benzothiazolinones such as 5-chloro-3-(4-hydroxypiperidinocarbonylmethyl)benzothiazoline-2-one are known as anti-allergic agents (see U.S. Pat. No. 3,755,327). 
     SUMMARY OF THE INVENTION 
     This invention provides a benzothiazolinone derivative of the formula (I): ##STR2## in which A is a lower alkylene group; X is a halogen atom, R is the group --OR 1 , --NR 2  R 3  or --R 4  --COOH wherein R 1  is a lower alkyl group, R 2  and R 3  are hydrogen atom or a lower alkyl group and R 4  is a lower alkylene or alkenylene group, and pharmaceutically acceptable salt thereof. 
     The invention also provides the production and phamaceutical composition of the above mentioned compounds. 
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     In the above formula (I), the lower alkylene group for the symbols A and R 4  means a straight chain or branched chain alkylene group containing 1 to 6 carbon atoms, such as methylene, propylene, trimethylene, tetramethylene, pentamethylene or hexamethylene. The lower alkenylene group for the symbol R 4  means a straight chain or branched chain alkenylene group containing 2 to 6 carbon atoms, such as vinylene, propenylene, butenylene, pentenylene, hexenylene or 2-methyl-3-butenylene. The lower alkyl group for the symbols R 1 , R 2  and R 3  means a straight chain or branched chain alkyl group containing 1 to 6 carbon atoms, such as methyl, ethyl, propyl, butyl, pentyl, hexyl, isopropyl, isobutyl or tertiary butyl. The halogen atom for the symbol X may be fluorine, chlorine, bromine or iodine atom. 
     A preferred alkylene group is methylene group and a preferred halogen atom is chlorine atom which can exist at the 4, 5, 6 or 7th position of the benzothiazoline ring. Preferred examples of the group --OCO--R in the formula (I) are ethoxycarbonyloxy, N-methylcarbamoyloxy, N-ethylcarbamoyloxy N,N-dimethylcarbamoyloxy, carbamoyloxy, carboxyethylcarbonyloxy and carboxyvinylcarbonyloxy, which are preferred to be at the 3 or 4th position of the piperidine ring. 
     Examples of the pharmaceutically acceptable salts of the benzothiazolinone derivatives (I) include alkali metal salts such as sodium salt and potassium salt, alkaline earth metal salts such as calcium salt and magnesium salt and ammonium salt. 
     The benzothiazoline derivatives (I) can be prepared by the following methods. 
     Preparation method 1: ##STR3## 
     Preparation method 2: ##STR4## 
     Preparation method 3: ##STR5## 
     Preparation method 4: ##STR6## 
     In the above reaction formulae, the symbols A, X, R 1 , R 2 , R 3  and R 4  have the same meanings as defined above, Ra 2  is a lower alkyl group and Z is a halogen atom. 
     Details of the above mentioned methods are as follows. 
     Preparation method 1: 
     The benzothiazolinone derivatives (Ia) can be prepared by reacting the starting compound (II) or its salt with the starting compound (III). 
     The compound (II) can be prepared by the method disclosed in U.S. Pat. No. 3,755,327. 
     Examples of the salts of the starting compound (II) include alkali metal salts such as sodium salt, potassium salt and lithium salt. 
     Examples of the halogen atoms for Z of the starting compounds (III) include the same atoms as defined above. 
     This reaction can be generally carried out in a solvent such as pyridine, dimethylaniline, trimethylamine, dioxane, tetrahydrofuran or N,N-dimethylformamide with warming or heating. 
     Preparation method 2: 
     The benzothiazolinone derivatives (Ib) can be prepared by reacting the starting compound (II) or its salt with the starting compound (IV). 
     This reaction can be generally carried out in a solvent such as pyridine, tetrahydrofuran, dioxane or N,N-dimethylformamide with warming or heating. 
     Preparation method 3: 
     The benzothiazolinone derivative (Ic) can be prepared by reacting the starting compound (II) or its salt with phosgene or trichloromethylchloroformate to obtain the intermediate (V), followed by the reaction with the starting compound (VI) or its salt. 
     Examples of the salts of the starting compound (VI) include salts with inorganic acids such as hydrochloric acid, nitric acid and sulphuric acid. 
     The first stage in the above reactions can be generally carried out in a solvent such as dioxane, tetrahydrofuran or N,N-dimethylformamide with warming or heating. 
     The second stage can be also generally carried out in a solvent such as dioxane, tetrahydrofuran or N,N-dimethylformamide with warming or heating. When the salt of the starting compound (VI) is used in this stage, the reaction is carried out in the presence of a base such as sodium hydroxide, potassium hydroxide, pyridine, trimethylamine, triethylamine or 4-dimethylaminopyridine. 
     Preparation method 4: 
     The benzothiazolinone derivative (Id) and its salt can be prepared by reacting the starting compound (II) with the starting compound (VII) or its reactive derivative. 
     Examples of the reactive derivatives of the starting materials (VII) include acid halides, acid active esters, acid anhydride and the like. 
     This reaction can be generally carried out in the presence of a base such as sodium hydroxide, potassium hydroxide, pyridine, trimethylamine, triethylamine or 4-dimethylaminopyridine, in a solvent such as tetrahydrofuran, dioxane or N,N-dimethylformamide with warming or heating. 
     The object compounds obtained by the above methods can be isolated and purified in a conventional manner. 
     The benzothiazoline derivatives (Ia), (Ib), (Ic), and (Id) all are included in the object compounds (I). Consequently, the salts of the benzothiazolinone derivatives (I) as examplified above are applicable to the salts of the benzothiazolinone derivatives (Id). 
     The benzothiazolinone derivatives (I) and pharmaceutically acceptable salts thereof may be administered in the form of conventional pharmaceutical preparations suitable for oral or parenteral administration or topical application, such as capsules, granules, powders, tablets, ointments, syrups, injections, suppositories, aerosols, inhalants or eye drops. These pharmaceutical preparations may be prepared in a conventional method, using conventional additives for preparations. 
     Examples of the additives for preparations include carrieres such as sucrose, lactose, glucose, starch, D-mannitol, D-sorbitol, crystalline cellulose, hydroxypropyl starch, talc, sodium hydrogenphosphate and calcium carbonate; binders such as methylcellulose, ethylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, polyvinyl pyrrolidone, methylmethacrylate-methacrylic acid copolymer, gelatin, gum arabic, sucrose and starch; disintegrating agents such as starch, carboxymethyl cellulose or its calcium salt, hydroxypropyl cellulose with lower degree of substitution, croscarmellose sodium A type (Ac-Di-Sol), hydroxypropyl starch, crystalline cellulose and sodium lauryl sulfate; lubricants such as magnesium stearate, calcium stearate, talc and hydrated silicon dioxide; corrigents such as citric acid, menthol, ammonium glycyrrhetinate, glycine and orange powders; preservatives such as sodium benzoate, sodium hydrogen sulfite, methyl p-oxybenzoate and propyl p-oxybenzoate; stabilizers such as citric acid, sodium citrate and acetic acid; suspending agents such as methyl cellulose, polyvinyl pyrrolidone, carboxymethyl cellulose or its calsium salt and crystalline cellulose; dispersing agents such as polysorbate 80, Emalgen 408 (surfactant) and Emasol 310 (surfactant); solvents such as water; and bases such as cocoa butter, polyethyleneglycol, witepsol, white petrolatum and carboxyvinyl polymer. 
     These additives can be suitably chosen depending on the nature of the preparations. 
     The administration dosages of the benzothiazolinone derivatives and salts thereof are varied considerably taken into account the age and symptom of the subject and the manner for administration. When administered by oral route, injection or inhalation, the most suitable dosage can be chosen from the range of 0.1 to 1000 mg/kg/day, preferably 0.25 to 200 mg/kg/day. When administered topically by ointment, inhalation, etc, the dosage is determined considerably taken into account absorption by the tissue of the active ingredient from the preparation. Examples of dosage units of tablets are 50, 100, 200 mg, etc.; injections in intravenous route 12.5, 25 mg, etc. and that in subcutaneous route or in intramuscle route 15, 30 mg, etc.; suppository about 500 mg; and ointment about 100 to 2000 mg. 
     In addition to the compounds which will be described in the following examples, interesting compounds of the invention may be listed as follows. 
     4-chloro-3-(4-ethoxycarbonyloxypiperidinocarbonylmethyl)benzothiazolin-2-one, 
     6-chloro-3-(4-ethoxycarbonyloxypiperidinocarbonylmethyl)benzothiazolin-2-one, 
     7-chloro-3-(4-ethoxycarbonyloxypiperidinocarbonylmethyl)benzothiazolin-2-one, 
     4-chloro-3-[4-(N-methylcarbamoyloxy)piperdinocarbonylmethyl]benzothiazolin-2-one, 
     6-chloro-3-[4-(N-methylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one, 
     7-chloro-3-[4-(N-methylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one, 
     4-chloro-3-[4-(N-ethylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one, 
     6-chloro-3-[4-(N-ethylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one, 
     7-chloro-3-[4-(N-ethylcarbamoyloxy)piperdinocarbonylmethyl]benzothiazolin-2-one, 
     4-chloro-3-(4-carbamoyloxypiperidinocarbonylmethyl)benzothiazolin-2-one, 
     6-chloro-3-(4-carbamoyloxypiperidinocarbonylmethyl)benzothiazolin-2-one, 
     7-chloro-3-(4-carbamoyloxypiperidinocarbonylmethyl)benzothiazolin-2-one, 
     4-chloro-3-[4-(N,N-dimethylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one, 
     6-chloro-3-[4-(N,N-dimethylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one, 
     7-chloro-3-[4-(N,N-dimethylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one, 
     3-[1-(4-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-3-yl-oxycarbonyl]propionic acid, 
     3-[1-(6-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-3-yl-oxycarbonyl]propionic acid, 
     3-[1-(7-chloro-2-oxobenzothiazolin-3yl-acetyl)piperidin-3-yl-oxycarbonyl]propionic acid, 
     3-[1-(4-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]propionic acid, 
     3-[1-(6-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]propionic acid, 
     3-[1-(7-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]propionic acid, 
     3-[1-(4-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]acrylic acid, 
     3-[1-(6-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]acrylic acid and 
     3-[1-(7-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]acrylic acid, and sodium or potassium salt thereof when carboxyl group is contained in the molecule of the above-mentioned compounds. 
     (Effect) 
     The antiallergic and anti-inflammatory activities of the benzothiazolinone derivative (I) of the invention are described below. 
     TEST EXAMPLE 1 
     Antagonism to passive cutaneous anaphylaxis (P.C.A.) in rat: 
     Test method 
     Antiserum was prepared by the following method. One mg of egg albumin emulsified in 0.5 ml of Diphtheria-Pertussis-Tetanus Combined Vaccine (Tanabe Seiyaku Company Ltd.) and 0.5 ml of Freund&#39;s incomplete adjuvant (Seikagaku-Kogyo Company Ltd.) was used as the rat reaginic antiserum antigen against egg albumin. The emulsion was given subcutaneously in a single dose of 1 ml divided equally (0.25 ml) to the four foot pads of male SLC Wistar-strain rats aged 7 weeks, each weighing 170 to 190 g. Blood samples were collected 12 days after injection and centrifuged at 3000 r.p.m. at 4  C. for 15 minutes. The antisera thus obtained were stored at -20° C. 
     Groups consisting of five SLC Wistar-strain rats weighing 150 to 170 g were used. The rat reaginic antiserum against egg albumin was used at a dilution 1:4. The animals were sensitized with 0.1 ml of the antiserum injected intracutaneously on the depilated backs of the rats. Fortyeight hours after the injection of the antiserum, 1 ml of the mixture of egg albumin (5 mg) and Evan&#39;s blue (5 mg) in physiological saline (1 ml) was injected intravenously to cause the P.C.A. reaction. Thirty minutes after the injection of the antigen, animals were sacrified and the skin was removed. The blue spots which occured due to the P.C.A. reaction were measured their long and short diameters and the areas of the spots were found from the average of the values of the diameters. Each test compound was given orally with 16 mg/kg and intravenously with 4 mg/kg, 1 hour and 5 minutes before injection for P.C.A. reaction respectively. The reaction inhibitory ratio of the group administered the test compound was calculated from the ratio of the areas of the control group and the group administered the test compound. Test compounds are as follows. 
     Compound A: 3-[1-(5-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]propionic acid, 
     Compound B: 5-chloro-3-(4-ethoxycarbonyloxypiperidinocarbonylmethyl)benzothiazolin-2-one, 
     Compound C: 5-chloro-3-[4-(N-ethylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one, and 
     Compound D: 5-chloro-3-[4-(N,N-dimethylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one. 
     Test results 
     The results are shown by the following Table 1. 
     Table 1 [P.C.A. reaction inhibitory ratio of rat (%)] 
     
         ______________________________________                       IntraveneousTest compound       Oral administration                       administration______________________________________A           50.9            42.2B           50.7            44.2C           45.1            41.6D           40.3            47.8______________________________________ 
    
     TEST EXAMPLE 2 
     Antagonism to acute edema at sole of hind leg in rat: 
     Groups consisting of five SLC Wistar-strain rats weighing 150 to 170 g were used. 
     Each test compound was given orally with 200 mg/kg and intravenously with 64 mg/kg, 30 minutes and five minutes respectively before subcutaneous injection of a prophlogistic substance (1 ml of 1% λ-carrageenin solution) into the sole of one hind leg. A volume of the sole was measured with time. The edema ratio of sole edema was calculated in comparison between the volumes of the sole before and after injection of the prophlogistic substance. The inhibition ratio was calculated from the difference of the edema ratio between the substance administered group and the control group. 
     Test compounds 
     The compounds A and C in the test example 1 were used. 
     Results 
     The results are shown by the following Table 2. 
     Table 2 Inhibitory ratio (%) of sole edema in rat 
     
         ______________________________________Administrating route        Oral            IntravenousMeasuring time (hr.)        1       2       3     1     2______________________________________Compound A   24.5    62.3    69.4  83.2  24.2Compound C   --      --      --    64.9  52.9______________________________________ 
    
     As clear from the above test results, the benzothiazolinone derivatives (I) of the present invention have excellent antiallergic and anti-inflammatory properties. 
     The benzothiazolinone derivatives (I) and their salts of the present invention possess not only antiallergic and anti-inflammatory properties but also blood platelets clotting inhibiting property and are useful as drugs for treating various diseases, e.g., bronchial asthma, allergic coryza, inflammatory diseases, dermatic diseases such as urticaria, or diseases caused by thrombus formed in brain and heart. Further, the benzothiazolinone derivatives (Id) show good water solubility and can be conveniently used for preparing liquid preparations such as a collunarium or an injection. 
     (Examples) 
    
    
     This invention is illustrated in further detail by the following examples. 
     EXAMPLE 1 
     Preparation of 5-chloro-3-(4-ethoxycarbonyloxypiperidinocarbonylmethyl)benzothiazolin-2-one: 
     5-Chloro-3-(4-hydroxypiperidinocarbonylmethyl)benzothiazolin-2-one (3.2 g) was dissolved in dry pyridine (15 ml) and the solution was cooled. After ethyl chlorocarbonate (1.5 g) was dropwise added to the solution, the resultant was stirred for 8 hours at 80° C. Pyridine was evaporated off under vacuum from the reaction mixture and the residue was recrystallized from a mixture of chloroform and ethanol to yield the title compound as colorless crystals (3.2 g). mp 196°-197° C. 
     Elemental analysis for C 17  H 19  N 2  O 5  SCl 
     Calcd (%) C; 51.19, H; 4.80, N; 7.02. Found (%) C; 50.89, H; 4.50, N; 7.05. 
     NMR(CDCl 3 ) 
     δ: 
     1.35(3H, t, J=7 Hz), 1.55-2.30(4H, bs), 3.20-3.95(5H, bs), 4.22(2H, q, J=7 Hz), 4.69(2H, s), 6.85-8.30(3H, m) 
     EXAMPLE 2 
     Preparation of 5-chloro-3-[4-(N-methylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one: 
     5-Chloro-3-(4-hydroxypiperidinocarbonylmethyl)benzothiazolin-2-one (3.2 g) was dissolved in dry pyridine (15 ml) and the solution was cooled. After methyl isocyanate (0.9 g) was dropwise added to the solution, the resultant was stirred for 15 hours at 30° C. Pyridine was evaporated off under vacuum from the reaction mixture and the residue was recrystallized from methanol to yield the title compound as colorless crystals (2.7 g). mp 202°-203° C. 
     Elemental analysis for C 16  H 18  N 3  O 4  SCl 
     Calcd (%) C; 50.07, H; 4.73, N; 10.95. Found (%) C; 49,98, H; 4,50, N; 10.99. 
     NMR(CDCl 3 ) 
     δ: 
     1.50-2.20(4H, bs), 2.75(3H, d, J=5 Hz), 3.10-3.90(5H, bs), 4.60(2H, s), 6.70-7.15 (4H, m) 
     EXAMPLE 3 
     Preparation of 5-chloro-3[4-(N-ethylcarbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one: 5-Chloro-3-(4-hydroxypiperidinocarbonylmethyl)benzothiazolin-2-one (3.2 g) was dissolved in dry pyridine (15 ml) and the solution was cooled. After ethyl isocyanate (0.9 g) was dropwise added to the solution, the resultant was stirred for 10 hours at 50° C. Pyridine was evaporated off under vacuum from the reaction mixture and the residue was recrystallized from ethanol to yield the title compound as colorless crystals (3.0 g). mp 210°-211° C. 
     Elemental analysis for C 17  H 20  N 3  O 4  SCl 
     Calcd. (%) C; 51.32, H; 5.07, N; 10.56. Found (%) C; 51.11, H; 4.74, N; 10.58. 
     NMR(CDCl 3 ) 
     δ: 
     1.15(3H, t, J-7 Hz), 1.50-2.20(4H, bs), 3.00-4.00(7H, bs), 4.65(2H, s), 6.80-7.25(4H, m) 
     EXAMPLE 4 
     Preparation of 5-chloro-3-(4-carbamoyloxypiperidinocarbonylmethyl)benzothiazolin-2-one: 
     A solution of 5-chloro-3-(4-hydroxypiperidinocarbonylmethyl)benzothiazolin-2-one (3.2 g) in dry dioxane (70 ml) was gradually dropped in a phosgene solution in dry dioxane (10 ml), under cooling. The mixture was left for two hours at room temperature. After cooling, to the mixture was gradually dropwise added 28% aqueous ammonia (5 ml) and the resultant was stirred for two hours at room temperature. Dioxane was evaporated off under vacuum from the reaction mixture. The residue was washed with water and then recrystallized from a mixture of dioxane and ethanol to yield the title compound as colorless crystals (2.8 g). m.p. 210°-212° C. 
     Elemental analysis for C 15  H 16  N 3  O 4  SCl 
     Calcd. (%) C; 48.72, H; 4.36, N; 11.36. Found (%) C; 48.51, H; 4.23, N; 11.39. 
     NMR(DMSO-d 6 ) 
     δ: 
     1.10-2.30(4H, bs), 2.80-4.20(5H, bs), 4.97(2H, s), 6.49(2H, s), 7.00-7.90(3H, m) 
     EXAMPLE 5 
     Preparation of 5-chloro-3-[4-(N,N-dimethyl carbamoyloxy)piperdinocarbonylmethyl]benzothizolin-2-one: 
     A solution of 5-chloro-3-[4-hydroxypiperidinocarbonylmethyl]benzothiazolin-2-one (3.2 g) in dry tetrahydrofuran (60 ml) was cooled, to which a solution of trichloromethylchloroformate (0.61 ml) in dry tetrahydrofuran (2 ml) and a solution of triethylamine (1.4 ml) in dry tetrahydrofuran (4 ml) were dropwise added. After stirring for two hours at 60° C., the mixture was left overnight. After cooling the mixture, 50% aqueous dimethylamine (1.5 ml) was dropwise added to the mixture under stirring, followed by stirring for two hours at room temperature. Tetrahydrofuran was evaporated off under vacuum from the reaction mixture, and the residue was washed with water and then recrystallized from methanol to yield the title compound as colorless crystals (2.1 g). m.p. 148°-150° C. 
     Elemental analysis for C 17  H 20  N 3  O 4  SCl 
     Calcd. C; 51.32, H; 5.07, H; 10.56. Found: C; 51.11, H; 4.88, N; 10.68. 
     NMR(CDCl 3 ) 
     δ: 
     1.50-2.20(4H, bs), 2.90(6H, s), 3.20-4.00 (4H, bs), 4.66(2H, s), 4.75-5.10(1H, bs), 6.80-7.40(3H, m) 
     EXAMPLE 6 
     Preparation of 3-[1-(5-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-3-yl-oxycarbonyl]propionic acid: 
     A solution of 5-chloro-3-(3-hydroxypiperidinocarbonylmethyl)benzothiazolin-2-one (3.2 g) in dry pyridine (2.5 ml) and dry tetrahydrofuran (50 ml) was cooled. To the solution was dropwise added a solution of succinic anhydride (1.1 g) in dry tetrahydrofuran (5 ml) under stirring. The mixture was refluxed for two hours and then left overnight. The solvent was evaporated off under vacuum from the reaction mixture. The residue was dissolved in 5% aqueous sodium bicarbonate (20 ml). The resultant solution was cooled and adjusted to pH 2 with 1% hydrochloric acid. The precipitated crystals were collected by filtration, washed with water and then recrystallized from isopropyl alcohol to yield the title compound as colorless crystals (2.0 g). m.p. 131°-133° C. 
     Elemental analysis for C 18  H 19  N 2  O 6  SCl 
     Calcd. C; 50.65, H; 4.49, N; 6.56, Found: C; 50.39, H; 4.25, N; 6.77. 
     NMR(DMSO-d 6 ) 
     δ: 
     1.40-2.20(4H, bs), 2.62(4H, s), 3.00-4.50(5H, bs), 4.95(2H, s), 6.90-7.90(3H, m), 11.90(1H, bs) 
     The above compound (1.0 g) was dissolved in a solution of sodium bicarbonate (0.2 g) in water (10 ml) and water was distilled off under vacuum at below 50° C. to yield sodium salt of the title compound as colorless crystals. 
     NMR(DMSO-d 6 ) 
     δ: 
     1.40-2.20(4H, bs), 2.60(4H, s), 3.00-4.50(5H, bs), 4.93(2H, s), 6.90-7.90(3H, m) 
     EXAMPLE 7 
     Preparation of 3-[1-(5-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]propionic acid: 
     A solution of 5-chloro-3-(4-hydroxypiperidinocarbonylmethyl)benzothiazolin-2-one (3.2 g) in dry pyridine (2.5 ml) and dry tetrahydrofuran (50 ml) was cooled. To the solution was dropwise added a solution of succinic anhydride (1.1 g) in dry tetrahydrofuran (5 ml) under stirring. The mixture was refluxed for 2 hours and then left overnight. The reaction mixture was treated in the same manner as that in Example 6 to yield the title compound as colorless crystals (2.5 g). 
     m.p. 154°-156° C. 
     Elemental analysis for C 18  H 19  N 2  O 6  SCl 
     Calcd. (%) C; 50.65, H; 4.49, N; 6.56. Found (%) C; 50.54, H; 4.37, N; 6.53. 
     NMR(DMSO-d 6 ) 
     δ: 
     1.25-2.20(4H, bs), 2.53(4H, s), 3.10-4.00(5H, bs), 4.96(2H, s), 7.15-7.85(3H, m), 11.90(1H, bs) 
     The above compound was treated with sodium bicarbonate in the same manner as that in Example 6 to yield sodium salt of the title compound as colorless crystals. 
     NMR(DMSO-d 6 ) 
     δ: 
     1.25-2.20(4H, bs), 2.50(4H, s), 3.10-4.00(5H, bs), 4.92(2H, s), 7.15-7.85(3H, m) 
     EXAMPLE 8 
     Preparation of 3-[1-(5-chloro-2-oxobenzothiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]acrylic acid: 
     A solution of 5-chloro-3-(4-hydroxypiperidinocarbonylmetyl)benzothiazolin-2-one (3.2 g) in dry pyridine (2.5 ml) and dry tetrahydrofuran (50 ml) was cooled, to which a solution of maleic anhydride (1.0 g) in dry tetrahydrofuran (5 ml) was gradually dropwise added under stirring. The mixture was refluxed for two hours and then left overnight. The solvent was evaporated off under vacuum from the reaction mixture, and the residue was dissolved in 5% aqueous sodium bicarbonate (20 ml). The solution was cooled and adjusted to pH 2 with 1% hydrochloric acid. The precipitated crystals were collected by filtration, washed with water and then subjected to chromatography on silica gel eluting with ethanol/benzene (1:1). The solvent was evaporated off under vacuum from the eluate at below 50° C. The residue was recrystallized from isopropyl alcohol to yield the title compound as colorless crystals (2.1 g). m.p. 197°-198° C. 
     Elemental analysis for C 18  H 17  N 2  O 6  SCl 
     Calcd. (%) C; 50.89, H; 4.03, N; 6.59. Found: (%) C; 50.63, H; 4.21, N; 6.72. 
     NMR(DMSO-d 6 ) 
     δ: 
     1.50-2.30(4H, bs), 3.10-4.10(5H, bs), 4.95(2H, s), 5.87(1H, d, J=12 Hz), 6.55 (1H, d, J=12 Hz), 7.10-7.80(3H, m), 11.90(1H, bs) 
     The above compound was treated with sodium bicarbonate in the same manner as that in Example 6 to yield sodium salt of the title compound as colorless crystals. 
     NMR(DMSO-d 6 ) 
     δ: 
     1.50-2.30(4H, bs), 3.10-4.10(5H, bs), 4.97(2H, s), 5.89(1H, d, J=12 Hz), 6.57(1H, d, J=12 Hz), 7.10-7.80(3H, m) 
     EXAMPLE 9 
     
         ______________________________________3-[1-(5-chloro-2-oxobenzo                500    (parts by weight)thiazolin-3-yl-acetyl)piperidin-4-yl-oxycarbonyl]propionic acid (referredto as Compound A hereafter)Lactose              9250   &#34;Hydroxypropyl cellulose                200    &#34;Strach                50    &#34;______________________________________ 
    
     The above ingredients were blended and granulated in a conventional manner into granules. 
     EXAMPLE 10 
     
         ______________________________________5-Chloro-3-[4-(N,N--dimethyl              500     (parts by weight)carbamoyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one(referred to as Compound Dhereafter)Lactose            8500    &#34;Hydroxypropyl cellulose              200     &#34;Strach             500     &#34;Crystalline cellulose              300     &#34;______________________________________ 
    
     The above ingredients were blended and granulated in a conventional manner into small grains. 
     EXAMPLE 11 
     
         ______________________________________Compound A       500    (parts by weight)D-mannitol       1987   &#34;Magnesium stearate             3     &#34;______________________________________ 
    
     The above ingredients were blended and filled in hard gelatin-capsules, in a conventional manner, to give capsules, each of which contains 50 mg of Compound A. 
     EXAMPLE 12 
     
         ______________________________________Compound A        500    (parts by weight)Sucrose           9250   &#34;Citric acid       20     &#34;Hydroxypropyl cellulose             200    &#34;Sodium benzoate   50     &#34;______________________________________ 
    
     The above ingredients was blended in a conventional manner to make a dry-syrup. 
     EXAMPLE 13 
     
         ______________________________________5-Chloro-3-[4-(N--ethylcarba-              10000   (parts by weight)moyloxy)piperidinocarbonylmethyl]benzothiazolin-2-one (referred toas Compound C hereafter)Lactose            3000    &#34;Croscarmellose sodium A type(Ac--Di-Sol)       3000    &#34;Hydroxypropyl cellulose              1800    &#34;Magnesium stearate  200    &#34;______________________________________ 
    
     The above ingredients were blended and compressed, in a conventional manner, into tablets, each of which contains 100 mg of Compound C. 
     EXAMPLE 14 
     
         ______________________________________Compound A        20000    (parts by weight)Lactose           10400    &#34;Hydroxypropyl cellulose withlower degree of substitutionHydroxypropyl cellulose             1800     &#34;Magnesium stearate              200     &#34;______________________________________ 
    
     The above ingredients were blended and compressed, in a conventional manner, into tablets, each of which contains 200 mg of Compound A and then the tablets were coated with film-coating, in a conventional manner, to give film-coated tablets. 
     EXAMPLE 15 
     
         ______________________________________5-Chloro-3-(4-ethoxycarbonyloxy              5000    (parts by weight)piperidinocarbonylmethyl)benzothiazolin-2-one(referred to as Compound B)Lactose            4200    &#34;Hydroxypropyl cellulose with              1100    &#34;lower degree of substitutionHydroxypropyl cellulose               600    &#34;Magnesium stearate  100    &#34;______________________________________ 
    
     The above ingredients were blended and compressed, in a conventional manner, into tablets, each of which contains 50 mg of Compound B, and then the tablets were subjected to sugar-coating. 
     EXAMPLE 16 
     
         ______________________________________Compound A      25000     (parts by weight)Lactose         14600     &#34;Crystalline cellulose           4700      &#34;Ethyl cellulose  600      &#34;Hydroxypropyl cellulose           1800      &#34;Magnesium stearate            100      &#34;______________________________________ 
    
     The above ingredients were blended and granuled, in a conventional manner, into fast-soluble granules and sustained release granules. 
     These granules were compressed into tablets, each containing 250 mg of Compound A, and the tablets were subjected to film-coating in a conventional manner, to give film-coated sustained release tablets. 
     EXAMPLE 17 
     
         ______________________________________Compound A       1000   (parts by weight)Sodium bicarbonate            84     &#34;Citric acid      20     &#34;Methyl p-oxybenzoate            25     &#34;Propyl p-oxybenzoate            15     &#34;Sucrose          4500   &#34;______________________________________ 
    
     The above ingredients were dissolved in purified water to make a syrup of which total amount is 10,000 parts by weight. 
     EXAMPLE 18 
     
         ______________________________________Compound D       1000   (parts by weight)Citric acid      10     &#34;Crystalline cellulose            800    &#34;Methyl p-oxybenzoate            25     &#34;Propyl p-oxybenzoate            15     &#34;Sucrose          4500   &#34;______________________________________ 
    
     The above ingredients were suspended or dissolved in purified water to make a syrup of which total amount is 10,000 part by weight. 
     EXAMPLE 19 
     
         ______________________________________Compound A        2500   (parts by weight)Sodium hydroxide  40     &#34;Methyl p-oxybenzoate             10     &#34;Polysorbate 80    10     &#34;Lidocaine hydrochloride             50     &#34;______________________________________ 
    
     The above ingredients were dissolved in distilled water for injection to make injectable solution of which total amount is 10,000 parts by weight. 
     EXAMPLE 20 
     
         ______________________________________Compound A      500    (parts by weight)White petrolatum           9025   &#34;Sorbitan trioleate           475    &#34;______________________________________ 
    
     The above ingredients were blended and kneaded into ointment. 
     EXAMPLE 21 
     
         ______________________________________Compound A        5000   (parts by weight)White petrolatum 40000   &#34;Sorbitan sesquioleate             5000   &#34;Cetanol          18000   &#34;Lauromacrogol     500    &#34;Butyl p-oxylenzoate             100    &#34;______________________________________ 
    
     The above ingredients were blended and kneaded into ointment. 
     EXAMPLE 22 
     
         ______________________________________Compound A     250    (parts by weight)Lactic acid    250    &#34;______________________________________ 
    
     The above ingredients were blended and filled in capsules to give capsules, for inhalation, each of which contains 25 mg of Compound A. 
     EXAMPLE 23 
     
         ______________________________________Compound A       100    (parts by weight)Sodium bicarbonate            10     &#34;Polysorbate 80   40     &#34;Methyl p-oxybenzoate            26     &#34;Propyl p-oxybenzoate            14     &#34;______________________________________ 
    
     The above ingredients were dissolved in sterile purified water to make eyedrop of which total amount is 10,000 parts by weight.