Abstract:
To gain a better understanding of brain tumor angiogenesis, new techniques for isolating brain endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from brain ECS derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed genes that were specifically elevated in tumor-associated brain endothelium. These results confirm that neoplastic and normal endothelium in human brains are distinct at the molecular level, and have significant implications for the development of anti-angiogenic therapies in the future.

Description:
[0001]    This application claims the benefit of provisional application Ser. Nos. 60/403,390 filed Aug. 15, 2002 and 60/458,978 filed Apr. 1, 2003. The disclosures of each are expressly incorporated herein. 
     
    
     TECHNICAL FIELD OF THE INVENTION 
       [0002]    This invention is related to the area of angiogenesis and anti-angiogenesis. In particular, it relates to genes which are characteristically expressed in brain glioma endothelial cells. 
       BACKGROUND OF THE INVENTION 
       [0003]    Brain cancers represent an infrequent but deadly form of cancer that has seen little improvement in survivability over the last 30 years. Tumor excision followed by therapies relying on outdated cytotoxins and radiation inevitably results in a diminished quality of life. Gliomas represent the most common brain neoplasms with highly vascular and invasive characteristics defining gliomas as one of the most aggressive tumors known. Classifications of gliomas derive from both the cellular origin and staged aggressiveness. Derived from either astrocytes or oligodendrocytes, astrocytomas and oligodendrogliomas constitute the most common types of gliomas. As is common to other tumor type classifications, glioma increases in aggressiveness from the first to third stages of disease with stage IV, gliobastoma multiforme, being the most aggressive. Moreover, glioblastoma tumors constitute one of the most vascular tumors known. 
         [0004]    Vascular permeability within the brain is limited in comparison to other organs. Similarly, the accessibility of brain malignancies to immune surveillance was thought to be restricted as well although more recent evidence suggests the brain is not wholly immunologically privileged. This so called “blood-brain barrier” is thought to derive primarily from a combination of brain-specific capillary transport systems and astrocyte-regulated cross-talk with the endothelial cell-based vasculature (for reviews, see Bart, J., Groen, H. J., Hendrikse, N. H., van der Graaf, W. T., Vaalburg, W., and de Vries, E. G. (2000). The blood-brain barrier and oncology: new insights into function and modulation. Cancer Treat Rev 26, 449-62.) The presence of tight junctions and an observed high electrical resistance both contribute to restricted transvascular molecular exchange. The existence of a therapeutically impermeable vasculature has resulted in a comparatively limited amount of work aimed at intervening in brain malignancies and other CNS diseases. Defining proteins preferentially expressed on either normal or diseased brain endothelial cells holds promise for expanding CNS therapeutic regimens. 
         [0005]    The vascular microenvironment within gliomas has been studied primarily through morphological, circulatory and perfusion based experiments (for review see Vajkoczy, P., and Menger, M. D. (2000). Vascular microenvironment in gliomas. J Neurooncol 50, 99-108; and Bart, J., Groen, H. J., Hendrikse, N. H., van der Graaf, W. T., Vaalburg, W., and de Vries, E. G. (2000). The blood-brain barrier and oncology: new insights into function and modulation. Cancer Treat Rev 26, 449-62.) These studies demonstrate profound changes in vascualture architecture associated with tumor progression. Increased fenestrations, malperfusion, hyperpermeability, and reduced leukocyte-EC interaction are all phenotypic observations linked to glioma microvasculature Bernsen, H. J., Rijken, P. F., Oostendorp, T., and van der Kogel, A. J. (1995). Vascularity and perfusion of human gliomas xenografted in the athymic nude mouse. Br J Cancer 71, 721-6; Vick, N. A., and Bigner, D. D. (1972). Microvascular abnormalities in virally-induced canine brain tumors. Structural bases for altered blood-brain barrier function. J Neurol Sci 17, 29-39; and Hobbs, S. K., Monsky, W. L., Yuan, F., Roberts, W. G., Griffith, L., Torchilin, V. P., and Jain, R. K. (1998). Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci USA 95, 4607-12. It is also suggested that higher grade gliomas utilize intussuceptive capillary growth to a much larger degree than earlier staged gliomas that primarily utilize both sprouting an cooption to advance vessel growth. Vajkoczy, P., Schilling, L., Ullrich, A., Schmiedek, P., and Menger, M. D. (1998). Characterization of angiogenesis and microcirculation of high-grade glioma: an intravital multifluorescence microscopic approach in the athymic nude mouse. J Cereb Blood Flow Metab 18, 510-20. The molecular characterization of glioma ECs has thus far been limited to the evaluation of common growth factors or previously defined brain EC transporters. Holash, J., Maisonpierre, P. C., Compton, D., Boland, P., Alexander, C. R., Zagzag, D., Yancopoulos, G. D., and Wiegand, S. J. (1999). Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF. Science 284, 1994-8; Guerin, C., Wolff, J. E., Laterra, J., Drewes, L. R., Brem, H., and Goldstein, G. W. (1992). Vascular differentiation and glucose transporter expression in rat gliomas: effects of steroids. Aim Neurol 31, 481-7. 
         [0006]    To date, global gene expression profiles from endothelial cell-specific populations is limited to normal and tumorigenic colon tissue. St Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K. E., Montgomery, E., Lal, A., Riggins, G. J., Lengauer, C., Vogelstein, B., and Kinzler, K. W. (2000). Genes expressed in human tumor endothelium. Science 289, 1197-202. There is a need in the art for analysis of endothelial cells from other tissue, so that diagnostic and therapeutic for non-colonic tumors can be developed. 
       SUMMARY OF THE INVENTION 
       [0007]    According to one embodiment of the invention a method is provided to aid in diagnosing glioma. An expression product of at least one gene in a first brain tissue sample suspected of being neoplastic is detected. The at least one gene is selected from the group consisting of signal sequence receptor, delta (translocon-associated protein delta); DC2 protein; KIAA0404 protein; symplekin; Huntingtin interacting protein I; plasmalemma vesicle associated protein; KIAA0726 gene product; latexin protein; transforming growth factor, beta 1; hypothetical protein FLJ22215; Rag C protein; hypothetical protein FLJ23471; N-myristoyltransferase 1; hypothetical protein dJ1181N3.1; ribosomal protein L27; secreted protein, acidic, cysteine-rich (osteonectin); Hs 111988; Hs 112238; laminin, alpha 5; protective protein for beta-galactosidase (galactosialidosis); Melanoma associated gene; Melanoma associated gene; E3 ubiquitin ligase SMURF1; collagen, type N, alpha 1; collagen, type IV, alpha 1; collagen, type IV, alpha 1; insulin-like growth factor binding protein 7; gene predicted from cDNA with a complete coding sequence; Thy-1 cell surface antigen; Hs 127824; GTP binding protein 2;  Homo sapiens  mRNA; cDNA DKFZp586D0918 (from clone DKFZp586D0918); cutaneous T-cell lymphoma-associated tumor antigen se20-4; differentially expressed nucleolar TGF-beta1 target protein (DENTT); dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive); smoothelin; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); putative translation initiation factor; retinoic acid induced 14; matrix metalloproteinase 9 (gelatinase B, 92 kD gelatinase, 92 kD type IV collagenase); Lutheran blood group (Auberger b antigen included); stanniocalcin 2; nuclear factor (erythroid-derived 2)-like 2; protein tyrosine phosphatase, non-receptor type 1; integrin, alpha 10; collagen, type VI, alpha 2; chromosome 21 open reading frame 25; CDC37 (cell division cycle 37,  S. cerevisiae , homolog); Hs 16450; Rho guanine nucleotide exchange factor (GEF) 7; creatine kinase, brain; hypothetical protein FLJ10297; hypothetical protein FLJ10350; TNF-induced protein; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein); cofilin 1 (non-muscle); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); v-ets avian erythroblastosis virus E26 oncogene homolog 1; protease, cysteine, 1 (legumain); ribosomal protein L13; chromosome 22 open reading frame 5; zinc finger protein 144 (Mel-18); degenerative spermatocyte (homolog  Drosophila ; lipid desaturase); eukaryotic translation initiation factor 2C, 2; mitochondrial ribosomal protein 145; prostate tumor over expressed gene 1; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5 kD, B14.5a); glioma endothelial marker 1 precursor; NS1-binding protein; ribosomal protein L38; tuftelin-interacting protein; HLA class II region expressed gene KE2; translocase of inner mitochondrial membrane 17 homolog A (yeast); sudD (suppressor of bimD6,  Aspergillus nidulans ) homolog; heparan sulfate proteoglycan 2 (perlecan); SEC24 ( S. cerevisiae ) related gene family, member A; NADH dehydrogenase (ubiquinone) Fe—S protein 7 (20 kD) (NADH-coenzyme Q reductase); DNA segment on chromosome X and Y (unique) 155 expressed sequence; annexin A2;  Homo sapiens  clone 24670 mRNA sequence; hypothetical protein; matrix metalloproteinase 10 (stromelysin 2); KIAA 1049 protein; G protein-coupled receptor; hypothetical protein FLJ20401; matrix metalloproteinase 14 (membrane-inserted); KIAA0470 gene product; solute carrier family 29 (nucleoside transporters), member 1; stanniocalcin 1; stanniocalcin 1; stanniocalcin 1; tumor suppressor deleted in oral cancer-related 1; tumor suppressor deleted in oral cancer-related 1; apolipoprotein C—I; glutathione peroxidase 4 (phospholipid hydroperoxidase); Hs 272106; transcription factor binding to IGHM enhancer 3; hypothetical protein DKFZp762A227; hypothetical protein FLJ22362; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); PRO0628 protein; melanoma-associated antigen recognised by cytotoxic T lymphocytes; LOC88745;  Homo sapiens  beta-1,3-galactosyltransferase-6 (B3GALT6) mRNA, complete cds; sprouty (Drosophila) homolog 4; sprouty (Drosophila) homolog 4;  Homo sapiens  mRNA; cDNA DKFZp434E1515 (from clone DKFZp434E1515); coactosin-like protein; hypothetical protein FLJ21865; Hs296234; KIAA0685 gene product; hypothetical protein FLJ10980; ribosomal protein L10; ribosomal protein S19; Hs 299251; Huntingtin interacting protein K;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 50374; Hs 311780; Hs 212191; v-akt murine thymoma viral oncogene homolog 2; Hs 328774; transducin-like enhancer of split 2, homolog of  Drosophila  E(sp1); KIAA1870 protein; ribosomal protein L10a; peptidylprolyl isomerase A (cyclophilin A); Hs 344224; hypothetical protein FLJ23239; hypothetical protein DKFZp761H221; KIAA1887 protein;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 701679;  Homo sapiens  cDNA FLJ30634 fis, clone CTONG2002453;  Homo sapiens  cDNA FLJ32203 fis, clone PLACE6003038, weakly similar to ZINC FINGER PROTEIN 84;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 1035904; hypothetical protein L0057333; myosin ID; plexin B2; lectin, galactoside-binding, soluble, 8 (galectin 8); double ring-finger protein, Dorfin; DKFZP434B168 protein; LIM domain binding 2; integrin beta 4 binding protein; synaptopodin; Hs 54828; insulin induced gene 1; acetyl LDL receptor; SREC; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); hypothetical protein FLJ22329; schwannomin-interacting protein 1; PTEN induced putative kinase 1; myosin X;  Homo sapiens  cDNA FLJ32424 fis, clone SKMUS2000954, moderately similar to  Homo sapiens  F-box protein Fbx25 (FBX25) 97; golgi phosphoprotein 1; splicing factor, arginine/serine-rich 6; laminin, gamma 3; cysteine-rich protein 2; U6 snRNA-associated Sm-like protein LSm7; hypothetical protein FLJ10707;  Homo sapiens , Similar to RIKEN cDNA 2310012N15 gene, clone IMAGE:3342825, mRNA, partial cds; macrophage migration inhibitory factor (glycosylation-inhibiting factor); ubiquinol-cytochrome c reductase hinge protein; gap junction protein, alpha 1, 43 kD (connexin 43); dihydropyrimidinase-like 3; aquaporin 1 (channel-forming integral protein, 28 kD); protein expressed in thyroid; macrophage myristoylated alanine-rich C kinase substrate; procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers-Danlos syndrome type VI); protease, serine, 11 (IGF binding); 24-dehydrocholesterol reductase; collagen, type IV, alpha 2; profilin 1; apolipoprotein D; hyaluronoglucosaminidase 2; hypothetical protein FLJ22678; quiescin Q6; ras homolog gene family, member A; ras homolog gene family, member A; plasminogen activator, urokinase; insulin-like growth factor binding protein 3; uridine phosphorylase; KIAA0638 protein; B7 homolog 3; lamin A/C; lamin A/C; lamin A/C; regulator of G-protein signalling 12; proteasome (prosome, macropain) 26S subunit, non-ATPase, 8;  Homo sapiens , Similar to RIKEN cDNA 5730528L13 gene, clone MGC:17337 IMAGE:4213591, mRNA, complete cds; prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy); laminin, alpha 4; transcription elongation factor A (SII), 1; lectin, galactoside-binding, soluble, 3 binding protein; ribosomal protein S16; glycophorin C (Gerbich blood group); endothelin receptor type B; serine (or cysteine) proteinase inhibitor, Glade E (nexin, plasminogen activator inhibitor type 1), member 1; biglycan; small nuclear ribonucleoprotein polypeptide B″; transmembrane 4 superfamily member 2; TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28 kD; lysyl oxidase-like 2; SRY (sex determining region Y)-box 4; SOX4 SRY (sex determining region Y)-box 4; SRY (sex determining region Y)-box 4; actin related protein 2/3 complex, subunit 2 (34 kD);  Homo sapiens  cDNA: FLJ23507 fis, clone LNG03128; hypothetical protein FLJ12442; Fas (TNFRSF6)-associated via death domain; mitogen-activated protein kinase kinase kinase 11; TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal); insulin receptor; cell membrane glycoprotein, 110000M(r) (surface antigen);  Homo sapiens  cDNA FLJ11863 fis, clone HEMBA1006926; jagged 1 (Alagille syndrome); KIAA0304 gene product; pre-B-cell leukemia transcription factor 2;  Homo sapiens  cDNA FLJ31238 fis, clone KIDNE2004864; p53-induced protein; complement component 1, q subcomponent, receptor 1; complement component 1, q subcomponent, receptor 1; apolipoprotein E; chemokine (C—C motif) ligand 3; coagulation factor II (thrombin) receptor-like 3; coagulation factor III (thromboplastin, tissue factor); collagen, type I, alpha 1; collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant); C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; cystatin C (amyloid angiopathy and cerebral hemorrhage); endoplasmic reticulum associated protein 140 kDa; ESTs; ESTs; ESTs, Highly similar to hypothetical protein FLJ10350 [ Homo sapiens] [H.sapiens ]; ESTs, Highly similar to ITB1_HUMAN Integrin beta-1 precursor (Fibronectin receptor beta subunit) (CD29) (Integrin VLA-4 beta subunit) [ H.sapiens ]; ESTs, Weakly similar to hypothetical protein FLJ20489 [ Homo sapiens] [H.sapiens ]; ESTs, Weakly similar to T17346 hypothetical protein DKFZp586O1624.1—human (fragment) [ H.sapiens ]; ESTs, Weakly similar to T21371 hypothetical protein F25H8.3— Caenorhabditis elegans [C.elegans] ; eukaryotic translation initiation factor 4A, isoform 1; heme oxygenase (decycling) 1; Hermansky-Pudlak syndrome 4;  Homo sapiens  cDNA FLJ34888 fis, clone NT2NE2017332;  Homo sapiens  cDNA FLJ39848 fis, clone SPLEN2014669;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 1977059;  Homo sapiens , clone IMAGE:4845226, mRNA; hypothetical protein FLJ22329; hypothetical protein FLJ32205; hypothetical protein MGC4677; inhibin, beta B (activin AB beta polypeptide); insulin-like growth factor binding protein 5; junction plakoglobin; KIAA0620 protein; KIAA0943 protein; likely ortholog of rat vacuole membrane protein 1; Lysosomal-associated multispanning membrane protein-5; major histocompatibility complex, class I, B; major histocompatibility complex, class I, C; matrix Gla protein; matrix metalloproteinase 1 (interstitial collagenase); microtubule-associated protein 1 light chain 3 beta; nerve growth factor receptor (TNFR superfamily, member 16); ribosomal protein S9; ring finger protein 40; S100 calcium binding protein, beta (neural); sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; SPARC-like 1 (mast9, hevin); tumor necrosis factor, alpha-induced protein 3; UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5; von Willebrand factor; v-akt murine thymoma vial oncogene homolog 2; cyclin-dependent kinase (cdc2-like) 10; ortholog mouse myocytic induction/differentiation originator; brain-specific angiogenesis inhibitor 1; EGF-TM7 latrophilin-related protein; sema domain; integrin, alpha 5; likely ortholog of mouse fibronectin type III; Lutheran blood group (Auberger b antigen included); SSR4, TRAPD; nerve growth factor receptor (TNFR superfamily, member 16); insulin-like growth factor binding protein; leukemia inhibitory factor; protein tyrosine phosphatase, nonreceptor type I; and  Homo sapiens , clone IMAGE:3908182, mRNA, partial cds. Expression of the at least one gene in the first brain tissue sample is compared to expression of the at least one gene in a second brain tissue sample which is normal. Increased expression of the at least one gene in the first brain tissue sample relative to the second tissue sample identifies the first brain tissue sample as likely to be neoplastic. According to another embodiment of the invention a method is provided of treating a glioma. Cells of the glioma are contacted with an antibody. The antibody specifically binds to an extracellular epitope of a protein selected from the group consisting of plasmalemma associated protein; KIAA0726 gene product; osteonectin: laminin, alpha 5; collagen, type IV, alpha 1; insulin-like growth factor binding protein 7; Thy-1 cell surface antigen; dysferlin, limb girdle muscular dystrophy 2B; integrin, alpha 5; matrix metalloproteinase 9; Lutjheran blood group, integrink, alpha 10, collagen, type VI, alpha 2; glioma endothelial marker 1 precursor; translocase of inner mitochondrial membrane 17 homolog A; heparan sulfate proteoglycan 2; annexin A2; matrix metalloproteinase 10; G protein-coupled receptor; matrix metalloproteinase 14; solute carrier family 29, member 1; CD59 antigen p18-20; KIAA 1870 protein; plexin B2; lectin, glactoside-binding, soluble, 8; integrin beta 4 binding protein; acetyl LDL receptor; laminin, gamma 3; macrophage migration inhibitory factor; gap junction protein, alpha 1, 43 kD; aquaporin 1; protease, serine, 11; collagen, type IV, alpha 2; apolipoprotein D; plasminogen activator, urokinase; insulin-like growth factor binding protein 3; regulator of G-protein signaling 12; prosaposin; laminin, alpha 4; lectin, galactoside-binding, soluble, 3 binding protein; glycophorin C; endothelin receptor type B; biglycan; transmembrane 4 superfamily member 2; lysyl osidase-like 2; TEK tyrosine kinase, endothelial; insulin receptore; cell membrane glycoprotein, 110000M(r); jagged 1; plasmalemma vesicle associated protein; TEM13, Thy-1 cell surface antigen; coagulation factor II (thrombin) receptor-like 3; dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive); sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); likely ortholog of rat vacuole membrane protein 1; nerve growth factor receptor (TNFR superfamily, member 16); degenerative spermatocyte homolog, lipid desaturase (Drosophila); TEM1, endosialin; heme oxygenase (decycling) 1; G protein-coupled receptor; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; matrix metalloproteinase 14 (membrane-inserted); solute carrier family 29 (nucleoside transporters), member 1; likely ortholog of mouse embryonic epithelial gene 1; major histocompatibility complex, class I, C; likely ortholog of mouse fibronectin type III repeat containing protein 1; sprouty homolog 4 (Drosophila); KIAA0620 protein; coagulation factor III (thromboplastin, tissue factor); aquaporin 1 (channel-forming integral protein, 28 kDa); major histocompatibility complex, class I, B; Lysosomal-associated multispanning membrane protein-5; endothelin receptor type B; insulin receptor; complement component 1, q subcomponent, receptor 1; brain-specific angiogenesis inhibitor 1; EGF-TM7 latrophilin-related protein; sema domain; integrin, alpha 5; likely ortholog of mouse fibronectin type III; Lutheran blood group (Auberger b antigen included); SSR4, TRAPD; nerve growth factor receptor (TNFR superfamily, member 16) and complement component 1, q subcomponent, receptor 1. Immune destruction of cells of the glioma is thereby triggered. 
         [0008]    According to still another embodiment of the invention a method is provided for identifying a test compound as a potential anti-cancer or anti-glioma drug. A test compound is contacted with a cell which expresses at least one gene selected from the group consisting of: signal sequence receptor, delta (translocon-associated protein delta); DC2 protein; KIAA0404 protein; symplekin; Huntingtin interacting protein I; plasmalemma vesicle associated protein; KIAA0726 gene product; latexin protein; transforming growth factor, beta 1; hypothetical protein FLJ22215; Rag C protein; hypothetical protein FLJ23471; N-myristoyltransferase 1; hypothetical protein dJ1181N3.1; ribosomal protein L27; secreted protein, acidic, cysteine-rich (osteonectin); Hs 111988; Hs 112238; laminin, alpha 5; protective protein for beta-galactosidase (galactosialidosis); Melanoma associated gene; Melanoma associated gene; E3 ubiquitin ligase SMURF1; collagen, type IV, alpha 1; collagen, type IV, alpha 1; collagen, type IV, alpha 1; insulin-like growth factor binding protein 7; gene predicted from cDNA with a complete coding sequence; Thy-1 cell surface antigen; Hs 127824; GTP binding protein 2;  Homo sapiens  mRNA; cDNA DKFZp586D0918 (from clone DKFZp586D0918); cutaneous T-cell lymphoma-associated tumor antigen se20-4; differentially expressed nucleolar TGF-beta1 target protein (DENTT); dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive); smoothelin; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); putative translation initiation factor; retinoic acid induced 14; matrix metalloproteinase 9 (gelatinase B, 92 kD gelatinase, 92 kD type IV collagenase); Lutheran blood group (Auberger b antigen included); stanniocalcin 2; nuclear factor (erythroid-derived 2)-like 2; protein tyrosine phosphatase, non-receptor type 1; integrin, alpha 10; collagen, type VI, alpha 2; chromosome 21 open reading frame 25; CDC37 (cell division cycle 37,  S. cerevisiae , homolog); Hs 16450; Rho guanine nucleotide exchange factor (GEF) 7; creatine kinase, brain; hypothetical protein FLJ10297; hypothetical protein FLJ10350; TNF-induced protein; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein); cofilin 1 (non-muscle); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); v-ets avian erythroblastosis virus E26 oncogene homolog 1; protease, cysteine, 1 (legumain); ribosomal protein L13; chromosome 22 open reading frame 5; zinc finger protein 144 (Mel-18); degenerative spermatocyte (homolog  Drosophila ; lipid desaturase); eukaryotic translation initiation factor 2C, 2; mitochondrial ribosomal protein L45; prostate tumor over expressed gene 1; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5 kD, B 14.5a); glioma endothelial marker 1 precursor; NS1-binding protein; ribosomal protein L38; tuftelin-interacting protein; HLA class II region expressed gene KE2; translocase of inner mitochondrial membrane 17 homolog A (yeast); sudD (suppressor of bimD6,  Aspergillus nidulans ) homolog; heparan sulfate proteoglycan 2 (perlecan); SEC24 ( S. cerevisiae ) related gene family, member A; NADH dehydrogenase (ubiquinone) Fe—S protein 7 (20 kD) (NADH-coenzyme Q reductase); DNA segment on chromosome X and Y (unique) 155 expressed sequence; annexin A2;  Homo sapiens  clone 24670 mRNA sequence; hypothetical protein; matrix metalloproteinase 10 (stromelysin 2); KIAA1049 protein; G protein-coupled receptor; hypothetical protein FLJ20401; matrix metalloproteinase 14 (membrane-inserted); KIAA0470 gene product; solute carrier family 29 (nucleoside transporters), member 1; stanniocalcin 1; stanniocalcin 1; stanniocalcin 1; tumor suppressor deleted in oral cancer-related 1; tumor suppressor deleted in oral cancer-related 1; apolipoprotein C—I; glutathione peroxidase 4 (phospholipid hydroperoxidase); Hs 272106; transcription factor binding to IGHM enhancer 3; hypothetical protein DKFZp762A227; hypothetical protein FLJ22362; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, 030, EL32 and G344); PRO0628 protein; melanoma-associated antigen recognised by cytotoxic T lymphocytes; LOC88745;  Homo sapiens  beta-1,3-galactosyltransferase-6 (B3GALT6) mRNA, complete cds; sprouty (Drosophila) homolog 4; sprouty (Drosophila) homolog 4;  Homo sapiens  mRNA; cDNA DKFZp434E1515 (from clone DKFZp434E1515); coactosin-like protein; hypothetical protein FLJ21865; Hs296234; KIAA0685 gene product; hypothetical protein FLJ10980; ribosomal protein L10; ribosomal protein S19; Hs 299251; Huntingtin interacting protein K;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 50374; Hs 311780; Hs 212191; v-akt murine thymoma viral oncogene homolog 2; Hs 328774; transducin-like enhancer of split 2, homolog of  Drosophila  E(sp1); KIAA1870 protein; ribosomal protein L10a; peptidylprolyl isomerase A (cyclophilin A); Hs 344224; hypothetical protein FLJ23239; hypothetical protein DKFZp761H221; KIAA1887 protein;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 701679;  Homo sapiens  cDNA F1130634 fis, clone CTONG2002453;  Homo sapiens  cDNA FLJ32203 fis, clone PLACE6003038, weakly similar to ZINC FINGER PROTEIN 84;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 1035904; hypothetical protein L0057333; myosin ID; plexin B2; lectin, galactoside-binding, soluble, 8 (galectin 8); double ring-finger protein, Dorfin; DKFZP434B168 protein; LIM domain binding 2; integrin beta 4 binding protein; synaptopodin; Hs 54828; insulin induced gene 1; acetyl LDL receptor; SREC; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); hypothetical protein FLJ22329; schwannomin-interacting protein 1; PTEN induced putative kinase 1; myosin X;  Homo sapiens  cDNA FLJ32424 fis, clone SKMUS2000954, moderately similar to  Homo sapiens  F-box protein Fbx25 (FBX25) 97; golgi phosphoprotein 1; splicing factor, arginine/serine-rich 6; laminin, gamma 3; cysteine-rich protein 2; U6 snRNA-associated Sm-like protein LSm7; hypothetical protein FLJ10707;  Homo sapiens , Similar to RIKEN cDNA 2310012N15 gene, clone IMAGE:3342825, mRNA, partial cds; macrophage migration inhibitory factor (glycosylation-inhibiting factor); ubiquinol-cytochrome c reductase hinge protein; gap junction protein, alpha 1, 43 kD (connexin 43); dihydropyrimidinase-like 3; aquaporin 1 (channel-forming integral protein, 28 kD); protein expressed in thyroid; macrophage myristoylated alanine-rich C kinase substrate; procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers-Danlos syndrome type VI); protease, serine, 11 (IGF binding); 24-dehydrocholesterol reductase; collagen, type IV, alpha 2; profilin 1; apolipoprotein D; hyaluronoglucosaminidase 2; hypothetical protein FLJ22678; quiescin Q6; ras homolog gene family, member A; ras homolog gene family, member A; plasminogen activator, urokinase; insulin-like growth factor binding protein 3; uridine phosphorylase; KIAA0638 protein; B7 homolog 3; lamin A/C; lamin A/C; lamin A/C; regulator of G-protein signalling 12; proteasome (prosome, macropain) 26S subunit, non-ATPase, 8;  Homo sapiens , Similar to RIKEN cDNA 5730528L13 gene, clone MGC:17337 IMAGE:4213591, mRNA, complete cds; prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy); laminin, alpha 4; transcription elongation factor A (SII), 1; lectin, galactoside-binding, soluble, 3 binding protein; ribosomal protein S16; glycophorin C (Gerbich blood group); endothelin receptor type B; serine (or cysteine) proteinase inhibitor, Glade E (nexin, plasminogen activator inhibitor type 1), member 1; biglycan; small nuclear ribonucleoprotein polypeptide B″; transmembrane 4 superfamily member 2; TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28 kD; lysyl oxidase-like 2; SRY (sex determining region Y)-box 4; SOX4 SRY (sex determining region Y)-box 4; SRY (sex determining region Y)-box 4; actin related protein 2/3 complex, subunit 2 (34 kD);  Homo sapiens  cDNA: FLJ23507 fis, clone LNG03128; hypothetical protein FLJ12442; Fas (TNFRSF6)-associated via death domain; mitogen-activated protein kinase kinase kinase 11; TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal); insulin receptor; cell membrane glycoprotein, 110000M(r) (surface antigen);  Homo sapiens  cDNA FLJ11863 fis, clone HEMBA1006926; jagged 1 (Alagille syndrome); KIAA0304 gene product; pre-B-cell leukemia transcription factor 2;  Homo sapiens  cDNA FLJ31238 fis, clone KIDNE2004864; p53-induced protein; complement component 1, q subcomponent, receptor 1; complement component 1, q subcomponent, receptor 1; apolipoprotein E; chemokine (C—C motif) ligand 3; coagulation factor II (thrombin) receptor-like 3; coagulation factor III (thromboplastin, tissue factor); collagen, type I, alpha 1; collagen, type IR, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant); C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; cystatin C (amyloid angiopathy and cerebral hemorrhage); endoplasmic reticulum associated protein 140 kDa; ESTs; ESTs; ESTs, Highly similar to hypothetical protein FLJ10350 [ Homo sapiens] [H.sapiens ]; ESTs, Highly similar to ITB1 HUMAN Integrin beta-1 precursor (Fibronectin receptor beta subunit) (CD29) (Integrin VLA-4 beta subunit) [ H.sapiens ]; ESTs, Weakly similar to hypothetical protein FLJ20489 [ Homo sapiens] [H.sapiens ]; ESTs, Weakly similar to T17346 hypothetical protein DKFZp586O1624.1-human (fragment) [ H.sapiens ]; ESTs, Weakly similar to T21371 hypothetical protein F25H8.3— Caenorhabditis elegans [C.elegans] ; eukaryotic translation initiation factor 4A, isoform 1; heme oxygenase (decycling) 1; Hermansky-Pudlak syndrome 4;  Homo sapiens  cDNA FLJ34888 fis, clone NT2NE2017332;  Homo sapiens  cDNA FLJ39848 fis, clone SPLEN2014669;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 1977059;  Homo sapiens , clone IMAGE:4845226, mRNA; hypothetical protein FLJ22329; hypothetical protein FLJ32205; hypothetical protein MGC4677; inhibin, beta B (activin AB beta polypeptide); insulin-like growth factor binding protein 5; junction plakoglobin; KIAA0620 protein; KIAA0943 protein; likely ortholog of rat vacuole membrane protein 1; Lysosomal-associated multispanning membrane protein-5; major histocompatibility complex, class I, B; major histocompatibility complex, class I, C; matrix Gla protein; matrix metalloproteinase 1 (interstitial collagenase); microtubule-associated protein 1 light chain 3 beta; nerve growth factor receptor (TNFR superfamily, member 16); ribosomal protein S9; ring finger protein 40; S100 calcium binding protein, beta (neural); sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; SPARC-like 1 (mast9, hevin); tumor necrosis factor, alpha-induced protein 3; UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5; von Willebrand factor; v-akt murine thymoma vial oncogene homolog 2; cyclin-dependent kinase (cdc2-like) 10; ortholog mouse myocytic induction/differentiation originator; brain-specific angiogenesis inhibitor 1; EGF-TM7 latrophilin-related protein; sema domain; integrin, alpha 5; likely ortholog of mouse fibronectin type III; Lutheran blood group (Auberger b antigen included); SSR4, TRAPD; nerve growth factor receptor (TNFR superfamily, member 16); insulin-like growth factor binding protein; leukemia inhibitory factor; protein tyrosine phosphatase, nonreceptor type I; and  Homo sapiens , clone IMAGE:3908182, mRNA, partial cds. An expression product of the at least one gene is monitored. The test compound is identified as a potential anti-cancer drug if it decreases the expression of the at least one gene. 
         [0009]    According to yet another embodiment of the invention a method is provided to aid in diagnosing glioma. An mRNA of at least one gene in a first brain tissue sample suspected of being neoplastic is detected. The at least one gene is identified by a tag selected from the group consisting of SEQ ID NO: 1-32. Expression of the at least one gene in the first brain tissue sample is compared to expression of the at least one gene in a second brain tissue sample which is normal. If increased expression of the at least one gene in the first brain tissue sample relative to the second tissue sample if found, the first brain tissue sample is identified as likely to be neoplastic. 
         [0010]    Another embodiment of the invention is a method of identifying a test compound as a potential anti-cancer or anti-glioma drug. A test compound is contacted with a cell. The cell expresses an mRNA of at least one gene identified by a tag selected from the group consisting of SEQ ID NO: 1-32. An mRNA of the at least one gene is monitored. The test compound is identified as a potential anti-cancer drug if it decreases the expression of at least one gene. 
         [0011]    Still another embodiment of the invention is a method to induce an immune response to glioma. A protein or nucleic acid encoding a protein is administered to a mammal, preferably a human. The protein is selected from the group consisting of: signal sequence receptor, delta (translocon-associated protein delta); DC2 protein; KIAA0404 protein; symplekin; Huntingtin interacting protein I; plasmalemma vesicle associated protein; KIAA0726 gene product; latexin protein; transforming growth factor, beta 1; hypothetical protein FLJ22215; Rag C protein; hypothetical protein FLJ23471; N-myristoyltransferase 1; hypothetical protein dJ1181N3.1; ribosomal protein L27; secreted protein, acidic, cysteine-rich (osteonectin); Hs 111988; Hs 112238; laminin, alpha 5; protective protein for beta-galactosidase (galactosialidosis); Melanoma associated gene; Melanoma associated gene; E3 ubiquitin ligase SMURF1; collagen, type IV, alpha 1; collagen, type IV, alpha 1; collagen, type IV, alpha 1; insulin-like growth factor binding protein 7; gene predicted from cDNA with a complete coding sequence; Thy-1 cell surface antigen; Hs 127824; GTP binding protein 2;  Homo sapiens  mRNA; cDNA DKFZp586D0918 (from clone DKFZp586D0918); cutaneous T-cell lymphoma-associated tumor antigen se20-4; differentially expressed nucleolar TGF-beta1 target protein (DENTT); dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive); smoothelin; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); putative translation initiation factor; retinoic acid induced 14; matrix metalloproteinase 9 (gelatinase B, 92 kD gelatinase, 92 kD type IV collagenase); Lutheran blood group (Auberger b antigen included); stanniocalcin 2; nuclear factor (erythroid-derived 2)-like 2; protein tyrosine phosphatase, non-receptor type 1; integrin, alpha 10; collagen, type VI, alpha 2; chromosome 21 open reading frame 25; CDC37 (cell division cycle 37,  S. cerevisiae , homolog); Hs 16450; Rho guanine nucleotide exchange factor (GEF) 7; creatine kinase, brain; hypothetical protein FLJ10297; hypothetical protein FLJ10350; TNF-induced protein; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein); cofilin 1 (non-muscle); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated); v-ets avian erythroblastosis virus E26 oncogene homolog 1; protease, cysteine, 1 (legumain); ribosomal protein L13; chromosome 22 open reading frame 5; zinc finger protein 144 (Mel-18); degenerative spermatocyte (homolog  Drosophila ; lipid desaturase); eukaryotic translation initiation factor 2C, 2; mitochondrial ribosomal protein L45; prostate tumor over expressed gene 1; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5 kD, B14.5a); glioma endothelial marker 1 precursor; NS1-binding protein; ribosomal protein L38; tuftelin-interacting protein; HLA class II region expressed gene KE2; translocase of inner mitochondrial membrane 17 homolog A (yeast); sudD (suppressor of bimD6,  Aspergillus nidulans ) homolog; heparan sulfate proteoglycan 2 (perlecan); SEC24 ( S. cerevisiae ) related gene family, member A; NADH dehydrogenase (ubiquinone) Fe—S protein 7 (20 kD) (NADH-coenzyme Q reductase); DNA segment on chromosome X and Y (unique) 155 expressed sequence; annexin A2;  Homo sapiens  clone 24670 mRNA sequence; hypothetical protein; matrix metalloproteinase 10 (stromelysin 2); KIAA1049 protein; G protein-coupled receptor; hypothetical protein FLJ20401; matrix metalloproteinase 14 (membrane-inserted); KIAA0470 gene product; solute carrier family 29 (nucleoside transporters), member 1; stanniocalcin 1; stanniocalcin 1; stanniocalcin 1; tumor suppressor deleted in oral cancer-related 1; tumor suppressor deleted in oral cancer-related 1; apolipoprotein C—I; glutathione peroxidase 4 (phospholipid hydroperoxidase); Hs 272106; transcription factor binding to IGHM enhancer 3; hypothetical protein DKFZp762A227; hypothetical protein FLJ22362; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 163A5, EJ16, EJ30, EL32 and G344); PRO0628 protein; melanoma-associated antigen recognised by cytotoxic T lymphocytes; LOC88745;  Homo sapiens  beta-1,3-galactosyltransferase-6 (B3GALT6) mRNA, complete cds; sprouty (Drosophila) homolog 4; sprouty (Drosophila) homolog 4;  Homo sapiens  mRNA; cDNA DKFZp434E1515 (from clone DKFZp434E1515); coactosin-like protein; hypothetical protein FLJ21865; Hs296234; KIAA0685 gene product; hypothetical protein FLJ10980; ribosomal protein L10; ribosomal protein S19; Hs 299251; Huntingtin interacting protein K;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 50374; Hs 311780; Hs 212191; v-akt murine thymoma viral oncogene homolog 2; Hs 328774; transducin-like enhancer of split 2, homolog of  Drosophila  E(sp1); KIAA1870 protein; ribosomal protein L10a; peptidylprolyl isomerase A (cyclophilin A); Hs 344224; hypothetical protein FLJ23239; hypothetical protein DKFZp761H221; KIAA1887 protein;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 701679;  Homo sapiens  cDNA FLJ30634 fis, clone CTONG2002453;  Homo sapiens  cDNA FLJ32203 fis, clone PLACE6003038, weakly similar to ZINC FINGER PROTEIN 84;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 1035904; hypothetical protein L0057333; myosin ID; plexin B2; lectin, galactoside-binding, soluble, 8 (galectin 8); double ring-finger protein, Dorfin; DKFZP434B 168 protein; LIM domain binding 2; integrin beta 4 binding protein; synaptopodin; Hs 54828; insulin induced gene 1; acetyl LDL receptor; SREC; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); hypothetical protein FLJ22329; schwannomin-interacting protein 1; PTEN induced putative kinase 1; myosin X;  Homo sapiens  cDNA FLJ32424 fis, clone SKMUS2000954, moderately similar to  Homo sapiens  F-box protein Fbx25 (FBX25) 97; golgi phosphoprotein 1; splicing factor, arginine/serine-rich 6; laminin, gamma 3; cysteine-rich protein 2; U6 snRNA-associated Sm-like protein LSm7 hypothetical protein FLJ10707;  Homo sapiens , Similar to RIKEN cDNA 2310012N15 gene, clone IMAGE:3342825, mRNA, partial cds; macrophage migration inhibitory factor (glycosylation-inhibiting factor); ubiquinol-cytochrome c reductase hinge protein; gap junction protein, alpha 1, 43 kD (connexin 43); dihydropyrimidinase-like 3; aquaporin 1 (channel-forming integral protein, 28 kD); protein expressed in thyroid; macrophage myristoylated alanine-rich C kinase substrate; procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers-Danlos syndrome type VI); protease, serine, 11 (IGF binding); 24-dehydrocholesterol reductase; collagen, type IV, alpha 2; profilin 1; apolipoprotein D; hyaluronoglucosaminidase 2; hypothetical protein FLJ22678; quiescin Q6; ras homolog gene family, member A; ras homolog gene family, member A; plasminogen activator, urokinase; insulin-like growth factor binding protein 3; uridine phosphorylase; KIAA0638 protein; B7 homolog 3; lamin A/C; lamin A/C; lamin A/C; regulator of G-protein signalling 12; proteasome (prosome, macropain) 26S subunit, non-ATPase, 8;  Homo sapiens , Similar to RIKEN cDNA 5730528L13 gene, clone MGC:17337 IMAGE:4213591, mRNA, complete cds; prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy); laminin, alpha 4; transcription elongation factor A (SII), 1; lectin, galactoside-binding, soluble, 3 binding protein; ribosomal protein S16; glycophorin C (Gerbich blood group); endothelin receptor type B; serine (or cysteine) proteinase inhibitor, Glade E (nexin, plasminogen activator inhibitor type 1), member 1; biglycan; small nuclear ribonucleoprotein polypeptide B″; transmembrane 4 superfamily member 2; TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 28 kD; lysyl oxidase-like 2; SRY (sex determining region Y)-box 4; SOX4 SRY (sex determining region Y)-box 4; SRY (sex determining region Y)-box 4; actin related protein 2/3 complex, subunit 2 (34 kD);  Homo sapiens  cDNA: FLJ23507 fis, clone LNG03128; hypothetical protein FLJ12442; Fas (TNFRSF6)-associated via death domain; mitogen-activated protein kinase kinase kinase 11; TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal); insulin receptor; cell membrane glycoprotein, 110000M(r) (surface antigen);  Homo sapiens  cDNA FLJ11863 fis, clone HEMBA1006926; jagged 1 (Alagille syndrome); KIAA0304 gene product; pre-B-cell leukemia transcription factor 2;  Homo sapiens  cDNA FLJ31238 fis, clone KIDNE2004864; p53-induced protein; complement component 1, q subcomponent, receptor 1; complement component 1, q subcomponent, receptor 1; apolipoprotein E; chemokine (C—C motif) ligand 3; coagulation factor II (thrombin) receptor-like 3; coagulation factor III (thromboplastin, tissue factor); collagen, type I, alpha 1; collagen, type III alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant); C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; cystatin C (amyloid angiopathy and cerebral hemorrhage); endoplasmic reticulum associated protein 140 kDa; ESTs; ESTs; ESTs, Highly similar to hypothetical protein FLJ10350 [ Homo sapiens] [H.sapiens ]; ESTs, Highly similar to ITB1_HUMAN Integrin beta-1 precursor (Fibronectin receptor beta subunit) (CD29) (Integrin VLA-4 beta subunit) [ H.sapiens ]; ESTs, Weakly similar to hypothetical protein FLJ20489 [ Homo sapiens] [H.sapiens ]; ESTs, Weakly similar to T17346 hypothetical protein DKFZp58601624.1-human (fragment) [ H.sapiens ]; ESTs, Weakly similar to T21371 hypothetical protein F25H8.3— Caenorhabditis elegans [C.elegans] ; eukaryotic translation initiation factor 4A, isoform 1; heme oxygenase (decycling) 1; Hermansky-Pudlak syndrome 4;  Homo sapiens  cDNA FLJ34888 fis, clone NT2NE2017332;  Homo sapiens  cDNA FLJ39848 fis, clone SPLEN2014669;  Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 1977059;  Homo sapiens , clone IMAGE:4845226, mRNA; hypothetical protein FLJ22329; hypothetical protein FLJ32205; hypothetical protein MGC4677; inhibin, beta B (activin AB beta polypeptide); insulin-like growth factor binding protein 5; junction plakoglobin; KIAA0620 protein; KIAA0943 protein; likely ortholog of rat vacuole membrane protein 1; Lysosomal-associated multispanning membrane protein-5; major histocompatibility complex, class I, B; major histocompatibility complex, class I, C; matrix Gla protein; matrix metalloproteinase 1 (interstitial collagenase); microtubule-associated protein 1 light chain 3 beta; nerve growth factor receptor (TNFR superfamily, member 16); ribosomal protein S9; ring finger protein 40; S100 calcium binding protein, beta (neural); sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; SPARC-like 1 (mast9, hevin); tumor necrosis factor, alpha-induced protein 3; UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3; UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5; von Willebrand factor; v-akt murine thymoma vial oncogene homolog 2; cyclin-dependent kinase (cdc2-like) 10; ortholog mouse myocytic induction/differentiation originator; brain-specific angiogenesis inhibitor 1; EGF-TM7 latrophilin-related protein; sema domain; integrin, alpha 5; likely ortholog of mouse fibronectin type III; Lutheran blood group (Auberger b antigen included); SSR4, TRAPD; nerve growth factor receptor (TNFR superfamily, member 16); insulin-like growth factor binding protein; leukemia inhibitory factor; protein tyrosine phosphatase, nonreceptor type I; and  Homo sapiens , clone IMAGE:3908182, mRNA, partial cds. An immune response to the protein is thereby induced. 
         [0012]    The present invention thus provides the art with methods of diagnosing and treating gliomas and other brain tumors. 
     
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0013]    Using SAGE (Serial Analysis of Gene Expression) profiling, this study was able to identify previously unrecognized, angiogenesis-specific markers that discriminate between non-proliferative and pathologic endothelial cells. We identified 255 human genes that were expressed at significantly higher levels in brain tumor endothelium than in normal brain endothelium. See Table 1. We have named these markers GEMs (glioma endothelial markers). Any of the GEMs disclosed in any of the tables can be used in the methods of the present invention, according to the discretion of the skilled artisan. 
         [0014]    ECs represent only a minor fraction of the total cells within normal or tumor tissues, and only those EC transcripts expressed at the highest levels would be expected to be represented in libraries constructed from unfractionated tissues. The genes described in the current study should therefore provide a valuable resource for basic and clinical studies of human brain angiogenesis in the future. Genes which have been identified as expressed more in glioma endothelial cells than in normal brain endothelial cells (GEMs) include those which correspond to tags shown in SEQ ID NOS: 1-32. The tags correspond to the segment of the cDNA that is 3′ of the 3′ most restriction endonuclease site for the restriction enzyme NlaIII which was used as the anchoring enzyme. The tag shown is the same strand as the mRNA. Other such genes are listed in Tables 1 and 2. 
         [0000]    
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                   
                   
                   
                 SEQ 
                   
                   
               
               
                 StdTag 
                 SEQ 
                 LongTag 
                 ID 
                 Function 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 AAACCATTCT 
                 1 
                 AAACCATTCTCCTCCGC 
                 256 
                   
                   
               
               
                   
               
               
                 AAGGCAGGGA 
                 2 
                 AAGGCAGGGAGGGAGGG 
                 257 
               
               
                   
               
               
                 ACACAGCAAG 
                 1 
                 ACACAGCAAGACGAGAA 
                 258 
               
               
                   
               
               
                 AGCTGGAGTC 
                 4 
                 AGCTGGAGTCCTAGGCA 
                 259 
               
               
                   
               
               
                 AGCTGGCACC 
                 5 
                 AGCTGGCACCAGAGCCC 
                 260 
               
               
                   
               
               
                 ATAAATGAGG 
                 6 
                 ATAAATGAGGTAAGGTC 
                 261 
               
               
                   
               
               
                 CAAGCACCCC 
                 7 
                 CAAGCACCCCCGTTCCA 
                 262 
               
               
                   
               
               
                 CACTACCCAC 
                 8 
                 CACTACCCACCAGACGC 
                 263 
               
               
                   
               
               
                 CACTACTCAC 
                 9 
                 CACTACTCACCAGGCGC 
                 264 
               
               
                   
               
               
                 CCCACCTCCA 
                 10 
                 CCCACCTCCAGTCCAGC 
                 265 
               
               
                   
               
               
                 CCCGCCTCTT 
                 11 
                 CCCGCCTCTTCACGGGC 
                 266 
               
               
                   
               
               
                 CCTCAGATGT 
                 12 
                 CCTCAGATGTTTGAAAA 
                 267 
               
               
                   
               
               
                 CGCTACTCAC 
                 13 
                 CGCTACTCACCAGACGC 
                 268 
               
               
                   
               
               
                 CTAAGACCTC 
                 14 
                 CTAAGACCTCACCAGTC 
                 269 
               
               
                   
               
               
                 CTAAGACTTC 
                 15 
                 CTAAGACTTCACCGGTC 
                 270 
               
               
                   
               
               
                 GAGTGGGTGC 
                 16 
                 GAGTGGGTGCAGCCTCC 
                 271 
               
               
                   
               
               
                 GGGACAGCTG 
                 17 
                 GGGACAGCTGTCTGTGG 
                 272 
               
               
                   
               
               
                 GGGTTGGCTT 
                 18 
                 GGGTTGGCTTGAAACCA 
                 273 
               
               
                   
               
               
                 GTAAGTGTAC 
                 19 
                 GTAAGTGTACTGGAAGT 
                 274 
               
               
                   
               
               
                 GTAAGTGTAC 
                 20 
                 GTAAGTGTACTGGTAAG 
                 275 
               
               
                   
               
               
                 GTAAGGGGTAA 
                 21 
                 GTAGGGGTAAAAGGAGG 
                 276 
               
               
                   
               
               
                 TAACCACTGC 
                 22 
                 TAACCACTGCACTTTCC 
                 277 
               
               
                   
               
               
                 TACTGCTCGG 
                 23 
                 TACTGCTCGGAGGTCGG 
                 278 
               
               
                   
               
               
                 TCAGGCTGAA 
                 24 
                 TCAGGCTGAAGTCAGGC 
                 279 
               
               
                   
               
               
                 TCCATACACC 
                 25 
                 TCCATACACCTATCCCC 
                 280 
               
               
                   
               
               
                 TCCTTTTAAA 
                 26 
                 TCCTTTTAAAACAAAAC 
                 281 
               
               
                   
               
               
                 TGATTAAGGT 
                 27 
                 TGATTAAGGTCGGCGCT 
                 282 
               
               
                   
               
               
                 TGGTATCACA 
                 28 
                 TGGTATCACACAAGGGG 
                 283 
               
               
                   
               
               
                 TGGTGTATGC 
                 29 
                 TGGTGTATGCATCGGGG 
                 284 
               
               
                   
               
               
                 TGTCACTGGG 
                 30 
                 TGTCACTGGGCAGGCGG 
                 285 
               
               
                   
               
               
                 TGTGGGAGGC 
                 31 
                 TGTGGGAGGCTGATGGG 
                 286 
               
               
                   
               
               
                 TTTAACGGCC 
                 32 
                 TTTAACGGCCGCGGTAC 
                 287 
               
               
                   
               
               
                 GCTCTCTATG 
                 33 
                 GCTCTCTATGCTGACGT 
                 288 
                 sign sequence receptor, delta (translocon-associated protein delta) 
               
               
                   
               
               
                 AGAATGAAAC 
                 34 
                 AGAATGAAACTGCCGGG 
                 289 
                 DC2 protein 
               
               
                   
               
               
                 AAGTGGAATA 
                 35 
                 AAGTGGAATAAACTGCC 
                 290 
                 KIAA0404 protein 
               
               
                   
               
               
                 GATGACGACT 
                 36 
                 GATGACGACTCGGGGCTT 
                 291 
                 symplekin; Huntingtin interacting protein I 
               
               
                   
               
               
                 CCCTTTCACA 
                 37 
                 CCCTTTCACACACACTT 
                 292 
                 plasmalemma vesicle associated protein 
               
               
                   
               
               
                 TCCTGGGGCA 
                 38 
                 TCCTGGGGCAGGGGCGG 
                 293 
                 KIAA0726 gene product 
               
               
                   
               
               
                 TCTATTGATG 
                 39 
                 TCTATTGATGTGTATGC 
                 294 
                 latexin protein 
               
               
                   
               
               
                 GGGGCTGTAT 
                 40 
                 GGGGCTGTATTTAAGGA 
                 295 
                 transforming growth factor, beta 1 
               
               
                   
               
               
                 CCCAGGACAC 
                 41 
                 CCCAGGACACCAGCTGG 
                 296 
                 hypothetical protein FLJ22215 
               
               
                   
               
               
                 GGAGCTGCTG 
                 42 
                 GGAGCTGCTGCTTGTGG 
                 297 
                 Rag C protein 
               
               
                   
               
               
                 TGGACAGCAG 
                 43 
                 TGGACAGCAGGGACCTG 
                 298 
                 hypothetical protein FLJ23471 
               
               
                   
               
               
                 TCTGGGAACA 
                 44 
                 TCTGGGAACAGGGACGG 
                 299 
                 N-myristoyltransferase 1 
               
               
                   
               
               
                 CCTGTGTATG 
                 145 
                 CCTGTGTATGTGTGTAA 
                 300 
                 hypothetical protein dJ1181N3.1 
               
               
                   
               
               
                 GGCAAGAAGA 
                 46 
                 GGCAAGAAGAAGATCGC 
                 301 
                 ribosomal protein L27 
               
               
                   
               
               
                 AAATGCTTGG 
                 47 
                 AAATGCTTGGAGGTGAA 
                 302 
                 secreted protein, acidic, cysteine-rich (osteonectin) 
               
               
                   
               
               
                 CTAAAAACCT 
                 48 
                 CTAAAAACCTTATGACA 
                 303 
                 secreted protein, acidic, cysteine-rich (osteonectin) 
               
               
                   
               
               
                 GAGCATTGCA 
                 49 
                 GAGCATTGCACCACCCG 
                 304 
                 secreted protein, acidic, cysteine-rich (osteonectin) 
               
               
                   
               
               
                 GGTGGACACG 
                 50 
                 GGTGGACACGGATCTGC 
                 305 
                 secreted protein, acidic, cysteine-rich (osteonectin) 
               
               
                   
               
               
                 GCTCCTGAGC 
                 51 
                 GCTCCTGAGCCCCGGCC 
                 306 
                 ESTs, Weakly similar to 165992 gene MLL protein [ H.sapiens ] 
               
               
                   
               
               
                 AAGAAGTGGA 
                 52 
                 AAGAAGTGGAGATTGTC 
                 307 
                 ESTs 
               
               
                   
               
               
                 TGGGAAGTGG 
                 53 
                 TGGGAAGTGGGCTCCTT 
                 308 
                 maternally expressed 3 
               
               
                   
               
               
                 ACTCGCTCTG 
                 54 
                 ACTCGCTCTGTGGAGGT 
                 309 
                 laminin, alpha 5 
               
               
                   
               
               
                 TTTCAGGGGA 
                 55 
                 TTTCAGGGGAGGGGGAA 
                 310 
                 protective protein for beta-galactosidase (galactosialidosis) 
               
               
                   
               
               
                 ACAACGTCCA 
                 56 
                 ACAACGTCCAGCTGGTG 
                 311 
                 Melanoma associated gene 
               
               
                   
               
               
                 GTCTCAGTGC 
                 57 
                 GTCTCAGTGCTGAGGCG 
                 312 
                 Melanoma associated gene 
               
               
                   
               
               
                 CCCCCTGCCC 
                 58 
                 CCCCCTGCCCCTCTGCC 
                 313 
                 E3 ubiquitin ligase SMURF1 
               
               
                   
               
               
                 AGAAACCACG 
                 59 
                 AGAAACCACGGAAATGG 
                 314 
                 collagen, type IV, alpha 1 
               
               
                   
               
               
                 GACCGCAGGA 
                 60 
                 GACCGCAGGAGGGCAGA 
                 315 
                 collagen, type IV, alpha 1 
               
               
                   
               
               
                 GTGCTACTTC 
                 61 
                 GTGCTACTTCTTCTTCT 
                 316 
                 collagen, type IV, alpha 1 
               
               
                   
               
               
                 GATAACTACA 
                 62 
                 GATAACTACATTACCTG 
                 317 
                 insulin-like growth factor binding protein 7 
               
               
                   
               
               
                 TGGCTGTGAC 
                 63 
                 TGGCTGTGACTGTGACT 
                 318 
                 gene predicted from cDNA with a complete coding sequence 
               
               
                   
               
               
                 GAGTGAGACC 
                 64 
                 GAGTGAGACCCAGGAGC 
                 319 
                 Thy-1 cell surface antigen 
               
               
                   
               
               
                 GAGTGGCTAC 
                 65 
                 GAGTGGCTACCCGCCGC 
                 320 
                 ESTs, Weakly similar to T28770 hypothetical protein W03D2.1- 
               
               
                   
                   
                   
                   
                 
                   Caenorhabditis elegans 
                 
               
               
                   
               
               
                 GACTCAGGGA 
                 66 
                 GACTCAGGGATTTGTTG 
                 321 
                 GTP binding protein 2 
               
               
                   
               
               
                 GTTATATGCC 
                 67 
                 GTTATATGCCCGGGAGA 
                 322 
                   Homo sapiens  mRNA; cDNA DKFZp586D0918 (from clone 
               
               
                   
               
               
                 GAGGCGCTGC 
                 68 
                 GAGGCGCTGCTGCCACC 
                 323 
                 cutaneous T-cell lymphoma-associated tumor antigen se20-4; 
               
               
                   
                   
                   
                   
                 differentially expressed nucleolar TGF-beta 1 target protein 
               
               
                   
                   
                   
                   
                 (DENTT) 
               
               
                   
               
               
                 GAGCTCTGAG 
                 69 
                 GAGCTCTGAGATCACCC 
                 324 
                 dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive) 
               
               
                   
               
               
                 GCCAGCCAGT 
                 70 
                 GCCAGCCAGTGGCAAGC 
                 325 
                 Smoothelin 
               
               
                   
               
               
                 ATGGCAACAG 
                 71 
                 ATGGCAACAGATCTGGA 
                 326 
                 integrin, alpha 5 (fibronectin receptor, alpha polypeptide) 
               
               
                   
               
               
                 AAGGAGTTAC 
                 72 
                 AAGGAGTTACACTAGTC 
                 327 
                 putative translation initiation factor 
               
               
                   
               
               
                 TCCCACAAGG 
                 73 
                 TCCCACAAGGCTGCTTG 
                 328 
                 retinoic acid induced 14 
               
               
                   
               
               
                 TAAATCCCCA 
                 74 
                 TAAATCCCCACTGGGAC 
                 329 
                 matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 
               
               
                   
                   
                   
                   
                 92kD type IV collagenase) 
               
               
                   
               
               
                 CCCGCCCCCG 
                 75 
                 CCCGCCCCCGCCTTCCC 
                 330 
                 Lutheran blood group (Auberger b antigen included) 
               
               
                   
               
               
                 CCCGAGGCAG 
                 76 
                 CCCGAGGCAGAGTCGGG 
                 331 
                 stanniocalcin 2 
               
               
                   
               
               
                 CTACGTGATG 
                 77 
                 CTACGTGATGAAGATGG 
                 332 
                 nuclear factor (erythroid-derived 2)-like 2 
               
               
                   
               
               
                 ATGGGTTTGC 
                 78 
                 ATGGGTTTGCATTTTAG 
                 333 
                 protein tyrosine phosphatase, non-receptor type 1 
               
               
                   
               
               
                 GGCATTGTCT 
                 79 
                 GGCATTGTCTCTGTTTC 
                 334 
                 integrin, alpha 10 
               
               
                   
               
               
                 GTGCTAAGCG 
                 80 
                 GTGCTAAGCGGGCCCGG 
                 335 
                 collagen, type VI, alpha 2 
               
               
                   
               
               
                 ACCGTTTGCA 
                 81 
                 ACCGTTTGCATTCGAAA 
                 336 
                 chromosome 21 open reading frame 25 
               
               
                   
               
               
                 CAGCGCTGCA 
                 82 
                 CAGCGCTGCATTGACTC 
                 337 
                 CDC37 (cell division cycle 37,  S. cerevisiae,  homolog) 
               
               
                   
               
               
                 GAAGACACTT 
                 83 
                 GAAGACACTTGGTTTGA 
                 338 
                 ESTs 
               
               
                   
               
               
                 CGCTGGGCGT 
                 84 
                 CGCTGGGCGTCTGGGAC 
                 339 
                 Rho guanine nucleotide exchange factor (GEF) 7 
               
               
                   
               
               
                 CACCCCTGAT 
                 85 
                 CACCCCTGATGTTCGCC 
                 340 
                 creatine kinase, brain 
               
               
                   
               
               
                 GCCCCCCTGC 
                 86 
                 GCCCCCCTGCCCCGTGC 
                 341 
                 hypothetic protein FLJ10297 
               
               
                   
               
               
                 CCCCCTGCCC 
                 87 
                 CCCCCTGCCCTCGCCTG 
                 342 
                 hypothetical protein FLJ10350 
               
               
                   
               
               
                 AGCATAAAAA 
                 88 
                 AGCATAAAAATGCGTGC 
                 343 
                 TNF-induced protein 
               
               
                   
               
               
                 GGGCTGGACG 
                 89 
                 GGGCTGGACGGCTGCGT 
                 344 
                 tumor necrosis factor receptor superfamily, member 12 
               
               
                   
                   
                   
                   
                 (translocating chain-association membrane protein) 
               
               
                   
               
               
                 CTGCCAACTT 
                 90 
                 CTGCCAACTTCTAACCG 
                 345 
                 cofillin 1 (non-muscle) 
               
               
                   
               
               
                 AAGTGGATAG 
                 91 
                 AAGTGGATAGATACTTC 
                 346 
                 splicing factor proline/glutamine rich (polypyrimidine tract- 
               
               
                   
                   
                   
                   
                 binding protein-associated) 
               
               
                   
               
               
                 CGTACTGAGC 
                 92 
                 CGTACTGAGCGCTTTGG 
                 347 
                 splicing factor proline/glutamine rich (polypyrimdine tract- 
               
               
                   
                   
                   
                   
                 binding protein-associated) 
               
               
                   
               
               
                 CCGCTTACTC 
                 93 
                 CCGCTTACTCTGTTGGG 
                 348 
                 v-ets avian erythroblastosis virus E26 oncogene homolog 1 
               
               
                   
               
               
                 GGGGCTTCTG 
                 94 
                 GGGGCTTCTGTAGCCCC 
                 349 
                 protease, cysteine, 1 (legumain) 
               
               
                   
               
               
                 CCCGTCCGGA 
                 95 
                 CCCGTCCGGAACGTCTA 
                 350 
                 ribosomal protein L13 
               
               
                   
               
               
                 AGTTCCACCA 
                 96 
                 AGTTCCACCAGAAAGCC 
                 351 
                 chromosome 22 open reading frame 5 
               
               
                   
               
               
                 GGCCTCCAGC 
                 97 
                 GGCCTCCAGCCACCCAC 
                 352 
                 zinc finger protein 144 (Mel-18) 
               
               
                   
               
               
                 GGAGGCTGAG 
                 98 
                 GGAGGCTGAGGTGGGAG 
                 353 
                 degenerative spermatocyte (homolog  Drosophila ; lipid desaturase) 
               
               
                   
               
               
                 CAGAGGCGTC 
                 99 
                 CAGAGGCGTCCGCAGGT 
                 354 
                 eukaryotic translation initiation factor 2C, 2 
               
               
                   
               
               
                 GACCAGCCTT 
                 100 
                 GACCAGCCTTCAGATGG 
                 355 
                 mitochondrial ribosomal protein L45 
               
               
                   
               
               
                 GAGGATGGTG 
                 101 
                 GAGGATGGTGTCCTGAG 
                 356 
                 prostate tumor over expressed gene 1 
               
               
                   
               
               
                 TCGTCGCAGA 
                 102 
                 TCGTCGCAGAAGGCGCT 
                 357 
                 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 
               
               
                   
                   
                   
                   
                 (14.5kD; B14.5a) 
               
               
                   
               
               
                 GGGGCTGCCC 
                 103 
                 GGGGCTGCCCAGCTGGA 
                 358 
                 tumor endothelial marker 1 precursor 
               
               
                   
               
               
                 CTGTACATAC 
                 104 
                 CTGTACATACTTTTTGG 
                 359 
                 NS1-binding protein 
               
               
                   
               
               
                 GCGACGAGGC 
                 105 
                 GCGACGAGGCGCGCTGG 
                 360 
                 ribosomal protein L38 
               
               
                   
               
               
                 GCCAAGTGAA 
                 106 
                 GCCAAGTGAACTGTGGC 
                 361 
                 tuftelin-interacting protein 
               
               
                   
               
               
                 AAGATAAACT 
                 107 
                 AAGATAAACTCTGGGCC 
                 362 
                 HLA class II region expressed gene KE2 
               
               
                   
               
               
                 GAGAGTGTAC 
                 108 
                 GAGAGTGTACTGGCACT 
                 363 
                 translocase of inner mitochondrial membrane 17 homolog A (yeast) 
               
               
                   
               
               
                 CCACTGCAGT 
                 109 
                 CCACTGCACTCCGGCCT 
                 364 
                 sudD (suppressor of bimD6,  Aspergillus nidulans ) homolog 
               
               
                   
               
               
                 CCACCCTCAC 
                 110 
                 CCACCCTCACACACACA 
                 365 
                 heparan sulfate proteoglycan 2 (perlecan) 
               
               
                   
               
               
                 CAGACCATTG 
                 111 
                 CAGACCATTGTTTGATC 
                 366 
                 SEC24 ( S. cerevisiae ) related gene family, member A 
               
               
                   
               
               
                 GGGATTTCTG 
                 112 
                 GGGAGCTGCGCCAACGG 
                 367 
                 NADH dehydrogenase (ubiquinone) Fe-S protein 7 (20kD) 
               
               
                   
                   
                   
                   
                 (NADH-coenzyme Q reductase) 
               
               
                   
               
               
                 GGGATTTCTG 
                 113 
                 GGGATTTCTGTGTCTGC 
                 368 
                 DNA segment on chromosome X and Y (unique) 155 expressed sequence 
               
               
                   
               
               
                 CTTCCAGCTA 
                 114 
                 CTTCCAGCTAACAGGTC 
                 369 
                 annexin A2 
               
               
                   
               
               
                 CAGAAACAGA 
                 115 
                 CAGAAACAGACTGGGGG 
                 370 
                   Homo sapiens  clone 24670 mRNA sequence 
               
               
                   
               
               
                 TCTGTGCTCA 
                 116 
                 TCTGTGCTCAGGAAGAG 
                 371 
                 hypothetical protein 
               
               
                   
               
               
                 TGCAATAGGT 
                 117 
                 TGCAATAGGTGAGAGAA 
                 372 
                 matrix metalloproteinase 10 (stromelysin 2) 
               
               
                   
               
               
                 ATGGCCAACT 
                 118 
                 ATGGCCAACTTCCACCT 
                 373 
                 KIAA1049 protein 
               
               
                   
               
               
                 TCACACAGTG 
                 119 
                 TCACACAGTGCCTGTCG 
                 374 
                 G protein-coupled receptor 
               
               
                   
               
               
                 GGCTTAGGAT 
                 120 
                 GGCTTAGGATGTGAATG 
                 375 
                 hypothetical protein FLJ20401 
               
               
                   
               
               
                 GGGAGGGGTG 
                 121 
                 GGGAGGGGTGGGGGTG 
                 376 
                 matrix metalloproteinase 14 (membrane-inserted) 
               
               
                   
               
               
                 GAAGTAGAAG 
                 122 
                 GAAGTAGAAGGTAAGGA 
                 377 
                 KIAA0470 gene product 
               
               
                   
               
               
                 CACCCTGTAC 
                 123 
                 CACCCTGTAGAGTTGCC 
                 378 
                 solute carrier family 29 (nucleoside transporters), member 1 
               
               
                   
               
               
                 ATGTTTACAA 
                 124 
                 ATGTTTACAAGATGGCG 
                 379 
                 stanniocalcin 1 
               
               
                   
               
               
                 CAAACTGGTC 
                 125 
                 CAAACTGGTCTAGGTCA 
                 380 
                 stanniocalcin 1 
               
               
                   
               
               
                 GTAATGACAG 
                 126 
                 GTAATGACAGATGCAAG 
                 381 
                 stanniocalcin 1 
               
               
                   
               
               
                 ACCTGCCGAC 
                 127 
                 ACCTGCCGACAGTGTTG 
                 382 
                 tumor suppressor deleted in oral cancer-related 1 
               
               
                   
               
               
                 TGATGCGCGC 
                 128 
                 TGATGCGCGCTTTGTTG 
                 383 
                 tumor suppressor deleted in oral cancer-related 1 
               
               
                   
               
               
                 TGGCCCCAGG 
                 129 
                 TGGCCCCAGGTGCCACC 
                 384 
                 apolipoprotein C-I 
               
               
                   
               
               
                 GCCTGCTGGG 
                 130 
                 GCCTGCTGGGCTTGGCT 
                 385 
                 glutathione peroxidase 4 (phospholipid hydroperoxidase) 
               
               
                   
               
               
                 TGCCTGTGGT 
                 131 
                 TGCCTGTGGTCCCAGCT 
                 86 
                 ESTs 
               
               
                   
               
               
                 GAGGGTATAC 
                 132 
                 GAGGGTATACTGAGGGG 
                 387 
                 transcription factor binding to IGHM enhancer 3 
               
               
                   
               
               
                 GGAGCCAGCT 
                 133 
                 GGAGCCAGCTGACCTGC 
                 388 
                 hypothetical protein DKFZp762A227 
               
               
                   
               
               
                 GAGCCTCAGG 
                 134 
                 GAGCCTCAGGTGCTCCC 
                 389 
                 hypothetical protein FLJ22362 
               
               
                   
               
               
                 TACTTCACAT 
                 135 
                 TACTTCACATACAGTGC 
                 390 
                 CD59 antigen p18-20 (antigen identified by monoclonal antibodies 
               
               
                   
                   
                   
                   
                 16.3A5, EJ16, EJ30, EL32 and G344) 
               
               
                   
               
               
                 TAATCCCAGC 
                 136 
                 TAATCCCAGCACTTTGG 
                 391 
                 PRO0628 protein 
               
               
                   
               
               
                 CACCTTCCAG 
                 137 
                 CACCTTCCAGCCCGGGG 
                 392 
                 melanoma-associated antigen recognised by cytotoxic T lymphocytes 
               
               
                   
               
               
                 GAGTCTGTTC 
                 138 
                 GAGTCTGTTCGTGACTC 
                 393 
                 LOC88745 
               
               
                   
               
               
                 GGATTTTGGT 
                 139 
                 GGATTTTGGTCTCTGTC 
                 394 
                   Homo sapiens  beta-1, 3-galactosyltransferase-6 (B3GALT6) mRNA 
               
               
                   
               
               
                 TGCCTGTAGT 
                 140 
                 TGCCTGTAGTCCTAGTT 
                 395 
                 sprouty ( Drosophila ) homolog 4 
               
               
                   
               
               
                 TTACAAACAG 
                 141 
                 TTACAAACAGAAAAGCT 
                 396 
                 sprouty ( Drosophila ) homolog 4 
               
               
                   
               
               
                 TCTTCTTTCA 
                 142 
                 TCTTCTTTCAGAATGGG 
                 397 
                   Homo sapiens  mRNA; cDNA DKFZp434E1515 (from clone 
               
               
                   
               
               
                 AGCACATTTG 
                 143 
                 AGCACATTTGATATAGC 
                 398 
                 coactosin-like protein 
               
               
                   
               
               
                 CAGGGCTCGC 
                 144 
                 CAGGGCTCGCGTGCGGG 
                 399 
                 hypothetical protein FLJ21865 
               
               
                   
               
               
                 GCTGGTCCCA 
                 145 
                 GCTGGTCCCAGGGCCAG 
                 400 
                 ESTs, Weakly similar to T31613 hypothetical protein 
               
               
                   
                   
                   
                   
                 Y50E8A.i- Caenorhabditis elegans  [ C.elegans ] 
               
               
                   
               
               
                 TCCACGCCCT 
                 146 
                 TCCACGCCCTTCCTGGC 
                 401 
                 K1AA0685 gene product 
               
               
                   
               
               
                 TTGCAATAGC 
                 147 
                 TTGCAATAGCAAAACCC 
                 402 
                 hypothetical protein FLJ10980 
               
               
                   
               
               
                 AGGGCTTCCA 
                 148 
                 AGGGCTTCCAATGTGCT 
                 403 
                 ribosomal protein L10 
               
               
                   
               
               
                 CTGGGTTAAT 
                 149 
                 CTGGGTTAATAAATTGC 
                 404 
                 ribosomal protein S19 
               
               
                   
               
               
                 AACCTGGGAG 
                 150 
                 AACCTGGGAGGTGGAGG 
                 405 
                 ESTs 
               
               
                   
               
               
                 GGCAACGTGG 
                 151 
                 GGCAACGTGGTAGAGGC 
                 406 
                 Huntingtin iteracting protein K 
               
               
                   
               
               
                 GGATGCGCAG 
                 152 
                 GGATGCGCAGGGGAGGC 
                 A07 
                   Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 50374 
               
               
                   
               
               
                 CACCTGTAGT 
                 153 
                 CACCTGTAGTCCTAGCT 
                 408 
                 EST 
               
               
                   
               
               
                 GTGGTGGGCG 
                 154 
                 GTGGTGGGCGCCTGTAG 
                 409 
                 EST 
               
               
                   
               
               
                 GCAGGGTGGG 
                 155 
                 GCAGGGTGGGGAGGGG 
                 410 
                 v-akt murine thymoma viral oncogene homolog 2 
               
               
                   
               
               
                 CAAGCATCCC 
                 156 
                 CAAGCATCCCCGTTCCA 
                 411 
                 EST 
               
               
                   
               
               
                 TGGGGGCCGA 
                 157 
                 TGGGGGCCGATGGGCAG 
                 412 
                 transducin-like enhancer of split 2, homolog of  Drosophila  E(sp1) 
               
               
                   
               
               
                 TCAGTGTATT 
                 158 
                 TCAGTGTATTAAAACCC 
                 413 
                 KIAA1870 protein 
               
               
                   
               
               
                 GGCAAGCCCC 
                 159 
                 GGCAAGCCCCAGCGCCT 
                 414 
                 ribosomal protein L10a 
               
               
                   
               
               
                 CCTAGCTGGA 
                 160 
                 CCTAGCTGGATTGCAGA 
                 415 
                 peptidylprolyl isomerase A (cyclophilin A) 
               
               
                   
               
               
                 GCAAAACCGT 
                 161 
                 GCAAAACCCTGCTCTCC 
                 416 
                 ESTs, Weakly similar to ubiquitous TPR motif, Y isoform [ H.sapiens ] 
               
               
                   
               
               
                 GCTGGTTCCT 
                 162 
                 GCTGGTTCCTGAGTGGC 
                 417 
                 hypothetical protein FLJ23239 
               
               
                   
               
               
                 GCACCTCAGC 
                 163 
                 GCACCTCAGCCAGGGGT 
                 418 
                 hypothetical protein DKFZp761H221 
               
               
                   
               
               
                 ACCAGCTGTC 
                 164 
                 ACCAGCTGTCCAGGGGC 
                 419 
                 KIAA1887 protein 
               
               
                   
               
               
                 TTTGAATCAG 
                 165 
                 TTTGAATCAGTGCTAGA 
                 420 
                   Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 701679 
               
               
                   
               
               
                 AGACTAGGGG 
                 166 
                 AGACTAGGGGCCGGAGC 
                 421 
                   Homo sapiens  cDNA FLJ30634 fis, clone CTONG2002453 
               
               
                   
               
               
                 AGCTCAGTGA 
                 167 
                 AGCTCAGTGAGAAGGGC 
                 422 
                   Homo sapiens  cDNA FLJ32203 fis, clone PLACE6003038, weakly similar 
               
               
                   
                   
                   
                   
                 to ZINC FINGER PROTEIN 84 
               
               
                   
               
               
                 GGCCAACATT 
                 168 
                 GGCCAACATTTGGTCCA 
                 423 
                   Homo sapiens  mRNA full length insert cDNA clone EUROIMAGE 1035904 
               
               
                   
               
               
                 TTTGTGGGCA 
                 169 
                 TTTGTGGGCAGTCAGGC 
                 424 
                 hypothetical protein LOC57333 
               
               
                   
               
               
                 ATTGTAGACA 
                 170 
                 ATTGTAGACAATGAGGG 
                 425 
                 myonsin ID 
               
               
                   
               
               
                 CCCTAGGTTG 
                 171 
                 CCCTAGGTTGGGCCCCT 
                 426 
                 plexin B2 
               
               
                   
               
               
                 AAATCACCAA 
                 172 
                 AATACAATCAAGGC 
                 427 
                 lectin, galactoside-binding, soiuble, 8 (galectin 8) 
               
               
                   
               
               
                 GGCTGCAGTC 
                 173 
                 GGCTGCAGTCTTCTTCC 
                 428 
                 double ring-finger protein, Dorfin 
               
               
                   
               
               
                 GTGGCAGGCG 
                 174 
                 GTGGCAGGCGCCTGTAG 
                 429 
                 DKFZP434B168 protein 
               
               
                   
               
               
                 TAAAGGCACA 
                 175 
                 TAAAGGCACAGTGGCTC 
                 430 
                 LIM domain binding 2 
               
               
                   
               
               
                 GGCTCCTGGC 
                 176 
                 GGCTCCTGGCTCTGGAC 
                 431 
                 integrin beta 4 binding protein 
               
               
                   
               
               
                 ATATTAGGAA 
                 177 
                 ATATTAGGAAGTCGGGG 
                 432 
                 Synaptopodin 
               
               
                   
               
               
                 GCTTCAGTGG 
                 178 
                 GCTTCAGTGGGGGAGAG 
                 433 
                 ESTs 
               
               
                   
               
               
                 TGATTAAAAC 
                 179 
                 TGATTAAAACAAGTTGC 
                 434 
                 insulin induced gene 1 
               
               
                   
               
               
                 AGCCACCACG 
                 180 
                 AGCCACCACGCCTGGTC 
                 435 
                 acetyl LDL receptor; SREC 
               
               
                   
               
               
                 GGCGGCTGCA 
                 181 
                 GGCGGCTGCAGAGCCTG 
                 436 
                 excision repair cross-complementing rodent repair deficiency, 
               
               
                   
                   
                   
                   
                 complementation group 1 (includes overlapping antisense sequence) 
               
               
                   
               
               
                 TGTTTGGGGG 
                 182 
                 TGTTTGGGGGCTTTTAG 
                 437 
                 hypothetical protein FLJ22329 
               
               
                   
               
               
                 CCTGCCTCGT 
                 183 
                 CCTGCCTCGTAGTGAAG 
                 438 
                 schwannomin-interactiing protein 1 
               
               
                   
               
               
                 AGGCCTGGGC 
                 184 
                 AGGCCTGGGCCTCTGCG 
                 439 
                 PTEN induced putative kinase 1 
               
               
                   
               
               
                 CAAAACTGTT 
                 185 
                 CAAAACTGTTTGTTGGC 
                 440 
                 myosin X 
               
               
                   
               
               
                 GAGAGGACAT 
                 186 
                 GAGAGGACATTGGAGGG 
                 441 
                   Homo sapiens  cDNA FLJ32424 fis, clone SKMUS2000954, moderately 
               
               
                   
                   
                   
                   
                 similar to  Homo sapiens  F-box protein Fbx25 (FBX25) 97 
               
               
                   
               
               
                 GAGTTAGGCA 
                 187 
                 GAGTTAGGCACTTCCTG 
                 442 
                 golgi phosphoprotein 1 
               
               
                   
               
               
                 CCGTAGTGCC 
                 188 
                 CCGTAGTGCCTTTATGG 
                 443 
                 splicing factor, arginine/serine-rich 6 
               
               
                   
               
               
                 CATAAACGGG 
                 189 
                 CATAAACGGGCACACCC 
                 444 
                 laminin, gamma 3 
               
               
                   
               
               
                 TCCCTGGCAG 
                 190 
                 TCCCTGGCAGAGGGCTT 
                 445 
                 cysteine-rich protein 2 
               
               
                   
               
               
                 GAGGCCATCC 
                 191 
                 GAGGCCATCCCCAACCC 
                 446 
                 U6 snRNA-associated Sm-like protein LSm7 
               
               
                   
               
               
                 TTGCCTGGGA 
                 192 
                 TTGCCTGGGATGCTGGT 
                 447 
                 hypothetical protein FLJ10707 
               
               
                   
               
               
                 CTGTCAGCGG 
                 193 
                 CTGTCAGCGGCTGCCCC 
                 448 
                   Homo sapiens,  Similar to RIKEN cDNA 2310012N15 gene, clone 
               
               
                   
                   
                   
                   
                 IMAGE:3342825, mRNA, partial cds 
               
               
                   
               
               
                 AACGCGGCCA 
                 194 
                 AACGCGGCCAATGTGGG 
                 449 
                 macrophage migration inhibitory factor (glycosylation-inhibiting 
               
               
                   
                   
                   
                   
                 factor) 
               
               
                   
               
               
                 GGTTTGGCTT 
                 195 
                 GGTTTGGCTTAGGCTGG 
                 450 
                 ubiqtanol-cytochrome c reductase hinge protein 
               
               
                   
               
               
                 GATTTTTGTG 
                 196 
                 GATTTTTGTGGTGTGGG 
                 451 
                 gap junction protein, alpha 1, 43kd (connexin 43) 
               
               
                   
               
               
                 GGCTGCCCTG 
                 197 
                 GGCTGCCCTGGGCAGCC 
                 452 
                 dihydropyrimidinase-like 3 
               
               
                   
               
               
                 ATGGCAACAG 
                 198 
                 ATGGAAAGAAACCAA 
                 453 
                 aquaporin 1 (channel-forming integral protein, 28kD) 
               
               
                   
               
               
                 CGCTGTGGGG 
                 199 
                 CGCTGTGGGGTGCAGAC 
                 454 
                 protein expressed in thyroid 
               
               
                   
               
               
                 GGCAGCCAGA 
                 200 
                 GGCAGCCAGAGCTCCAA 
                 455 
                 macrophage myristoylated alanine-rich C kinase substrate 
               
               
                   
               
               
                 AGAGCAAACC 
                 201 
                 AGAGCAAACCGTAGTCC 
                 456 
                 procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine 
               
               
                   
                   
                   
                   
                 hydroxylase, Ehlers-Danlos syndrome type VI) 
               
               
                   
               
               
                 TTTCCCTCAA 
                 202 
                 TTTCCCTCAAAGACTCT 
                 457 
                 protease, serine, 11 (IGF binding) 
               
               
                   
               
               
                 TCCCCGTGGC 
                 203 
                 TCCCCGTGGCTGTGGGG 
                 458 
                 24-dehydrocholesterol reductase 
               
               
                   
               
               
                 TTCTCCCAAA 
                 204 
                 TTCTCCCAAATACCGTT 
                 459 
                 collagen, type IV, alpha 2 
               
               
                   
               
               
                 GGCTGGGGGC 
                 205 
                 GGCTGGGGGCCAGGGCT 
                 460 
                 profilin 1 
               
               
                   
               
               
                 CCCTACCCTG 
                 206 
                 CCCTACCCTGTTACCTT 
                 461 
                 apolipoprotein D 
               
               
                   
               
               
                 TAGGACCCTG 
                 207 
                 TAGGACCCTGCAGGGGG 
                 462 
                 hyaluronoglucosaminidase 2 
               
               
                   
               
               
                 GTTTTTGCTT 
                 208 
                 GTTTTTGCTTCAGCGGC 
                 463 
                 hypothetical protein FLJ22678 
               
               
                   
               
               
                 CTTGATTCCC 
                 209 
                 CTTGATTCCCACGCTAC 
                 464 
                 quiescin Q6 
               
               
                   
               
               
                 GCTTGGCTCC 
                 210 
                 GCTTGGCTCCCAAAGGG 
                 465 
                 ras homolog gene family, member A 
               
               
                   
               
               
                 GGTGGCACTC 
                 211 
                 GGTGGCATCAGTCTCT 
                 466 
                 ras homolog gene family, member A 
               
               
                   
               
               
                 ACCTGTGACC 
                 212 
                 ACCTGTGACCAGCACTG 
                 467 
                 plasminogen activator, urokinase 
               
               
                   
               
               
                 ACTGAGGAAA 
                 213 
                 ACTGAGGAAAGGAGCTC 
                 468 
                 insulin-like growth factor binding protein 3 
               
               
                   
               
               
                 TGCAGCGCCT 
                 214 
                 TGCAGCGCCTGCGGCCT 
                 469 
                 uridine phosphorylase 
               
               
                   
               
               
                 CTGGGGGGAA 
                 215 
                 CTGGGGGGAAGGGACTG 
                 470 
                 KIAA0638 protein 
               
               
                   
               
               
                 GTGCTATTCT 
                 216 
                 GTGCTATTCTGGGGCTG 
                 471 
                 B7 homolog 3 
               
               
                   
               
               
                 GGAGGGGGCT 
                 217 
                 GGAGGGGGCTTGAAGCC 
                 472 
                 lamin A/C 
               
               
                   
               
               
                 GTGCCTGAGA 
                 218 
                 GTGCCTGAGAGGCAGGC 
                 473 
                 lamin A/C 
               
               
                   
               
               
                 TCACAGGGTC 
                 219 
                 TCACAGGGTCCCCGGGG 
                 474 
                 lamin A/C 
               
               
                   
               
               
                 GGGCTCCCTG 
                 220 
                 GGGCTCCCTGGCCCTGG 
                 475 
                 regulator of G-protein signalling 12 
               
               
                   
               
               
                 GCCCCAGGTA 
                 221 
                 GCCCCAGGTAGGGGGAC 
                 476 
                 proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 
               
               
                   
               
               
                 GAAAGTGGCT 
                 222 
                 GAAAGTGGCTGTCCTGG 
                 477 
                   Homo sapiens,  Similar to RIKEN cDNA 5730528L13 gene, clone 
               
               
                   
                   
                   
                   
                 MGC:17337 IMAGE:4213591, mRNA, complete cds 
               
               
                   
               
               
                 TCCCTGGCTG 
                 223 
                 TCCCTGGCTGTTGAGGC 
                 478 
                 prosaposin (variant Gaucher disease and variant metachromatic 
               
               
                   
               
               
                 ACAGAGCACA 
                 224 
                 ACAGAGCACAGCTGCCC 
                 479 
                 laminin, alpha 4 
               
               
                   
               
               
                 CTTTGCACTC 
                 225 
                 CTTTGCACTCTCCTTTG 
                 480 
                 transcription elongation factor A (SII), 1 
               
               
                   
               
               
                 ATGCTCCCTG 
                 226 
                 ATGCTCCTGAGGAGCT 
                 481 
                 lectin, galactoside-binding, soluble, 3 binding protein 
               
               
                   
               
               
                 CCGTCCAAGG 
                 227 
                 CCGTCCAAGGGTCCGCT 
                 482 
                 ribosomal protein S16 
               
               
                   
               
               
                 GGGCCCCCTG 
                 228 
                 GGGCCCCCTGGGCAGTG 
                 483 
                 glycophorin C (Gerbich blood group) 
               
               
                   
               
               
                 CTTATGCTGC 
                 229 
                 CTTATGCTGCTGGTGCC 
                 484 
                 endothelin receptor type B 
               
               
                   
               
               
                 GGTTATTTTG 
                 230 
                 GGTTATTTTGGAGTGTA 
                 485 
                 serine (or cysteine) proteinase inhibor, dade E (nexin, 
               
               
                   
                   
                   
                   
                 plasminogen activator inhibitor type 1), member 1 
               
               
                   
               
               
                 GCCTGTCCCT 
                 231 
                 GCCTGTCCCTCCAAGAC 
                 486 
                 Biglycan 
               
               
                   
               
               
                 AAGATGAGGG 
                 232 
                 AAGATGAGGGGGCAGGC 
                 487 
                 small nuclear ribonucleoprotein polypeptide B″ 
               
               
                   
               
               
                 CCAACAAGAA 
                 233 
                 CCAACAAGAATGCATTG 
                 488 
                 transmembrane 4 superfamily member 2 
               
               
                   
               
               
                 AAGGATGCGG 
                 234 
                 AAGGATGCGGTGATGGC 
                 489 
                 TAF11 RNA polymerase II, TATA box binding protein (TBP)-associated 
               
               
                   
                   
                   
                   
                 factor, 28 kD 
               
               
                   
               
               
                 TGTCATCACA 
                 235 
                 TGTCATCACAGACACTT 
                 490 
                 lysyl oxidase-like 2 
               
               
                   
               
               
                 CAGGCTTTTT 
                 236 
                 CAGGCTTTTTGGCTTCC 
                 491 
                 SRY (sex determining region Y)-box 4 
               
               
                   
               
               
                 TCAAGTTCAC 
                 237 
                 TCAAGTTCACTGCCTGT 
                 492 
                 SOX4 SRY (sex determining region Y)-box 4 
               
               
                   
               
               
                 TCCCTGGGCA 
                 238 
                 TCCCTGGGCAGCTTGAG 
                 493 
                 SRY (sex determining region Y)-box 4 
               
               
                   
               
               
                 CAGGAGTTCA 
                 239 
                 CAGGAGTTCAAAGAAGG 
                 494 
                 actin, related protein 2/3 complex, subunit 2 (34 kD) 
               
               
                   
               
               
                 CAGGTGGTTC 
                 240 
                 CAGGTGGTTCTGCCATC 
                 495 
                   Homo sapiens  cDNA: FLJ23507 fis, clone LNG03128 
               
               
                   
               
               
                 GCCCACATCC 
                 241 
                 GCCCACATCCGCTGAGG 
                 496 
                 hypothetical protein FLJ12442 
               
               
                   
               
               
                 GCTGGGGTGG 
                 242 
                 GCTGGGGTGGGGGTGG 
                 497 
                 Fas (TNFRSF6)-associated via death domain 
               
               
                   
               
               
                 GACCTCCTGC 
                 243 
                 GACCTCCTGCCCTGGGG 
                 498 
                 mitogen-activated protein kinase kinase kinase 11 
               
               
                   
               
               
                 AGTGAATAAA 
                 244 
                 AGTGAATAAATGTCTTG 
                 499 
                 TEK tyrosine kinase, endothelial (venous malformations, multiple 
               
               
                   
                   
                   
                   
                 cutaneous and mucosal) 
               
               
                   
               
               
                 AAGGTTCTTC 
                 245 
                 AAGGTTCTTCTCAAGGG 
                 500 
                 insulin receptor 
               
               
                   
               
               
                 AGCCTGGACT 
                 246 
                 AGCCTGGACTGAGCCAC 
                 501 
                 cell membrane glycoprotein, 110000M(r) (surface antigen) 
               
               
                   
               
               
                 CAACCCAGAT 
                 247 
                 CAACCCAGATTGGGGTG 
                 502 
                   Homo sapiens  cDNA FLJ11863 fis, clone HEMBA1006926 
               
               
                   
               
               
                 TGCTTCTGCC 
                 248 
                 TGCTTCTGCCACCCTGC 
                 503 
                 jagged 1 (Alagille syndrome) 
               
               
                   
               
               
                 CAGGTGACAA 
                 249 
                 CAGGTGACAAGGGCCCT 
                 504 
                 KIAA0304 gene product 
               
               
                   
               
               
                 GGCCGGGGGC 
                 250 
                 GGCCGGGGGCAGTTCTC 
                 505 
                 pre-B-cell leukemia transcription factor 2 
               
               
                   
               
               
                 GTGCGCTAGG 
                 251 
                 GTGCGCTAGGGCCCCGG 
                 506 
                   Homo sapiens  cDNA FLJ31238 fis, clone KIDNE2004864 
               
               
                   
               
               
                 AGGCTGTCCA 
                 252 
                 AGGCTGTCCAGGGTCTG 
                 507 
                 p53-induced protein 
               
               
                   
               
               
                 TGTTATGTCC 
                 253 
                 TGTTATGTCCATTTTGC 
                 508 
                 complement component 1, q subcomponent, receptor 1 
               
               
                   
               
               
                 TTTCCCAAAC 
                 254 
                 TTTCCCAAACTGTGAGG 
                 509 
                 complement component 1, q subcomponent, receptor 1 
               
               
                   
               
               
                 GGGGATGGGG 
                 255 
                 GGGGATGGGGTACTGCC 
                 510 
                   Homo sapiens,  clone IMAGE:3908182, mRNA, partial cds 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 2 
               
               
                   
               
               
                 SEQ ID NO: 
                 Unigene ID 
                 OMIMID 
                 gene symbol 
                 locuslink id 
                 Cellular Component 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 33 
                 Hs.102135 
                 300090 
                 SS 
                 6748 
                 endoplasmic reticulum, membrane 
               
               
                 34 
                 Hs.103180 
               
               
                 35 
                 Hs.105850 
                   
                 KIAA0404 
                 23130 
               
               
                 36 
                 Hs.107019 
                 602388 
                 SPK 
                 8189 
                 cytoplasm, nucleoplasm 
               
               
                 37 
                 Hs.107125 
                   
                   
                   
                 membrane 
               
               
                 38 
                 Hs.107809 
                   
                 KIAA0726 
                 9746 
                 membrane 
               
               
                 39 
                 Hs.109276 
               
               
                 40 
                 Hs.1103 
                 190180 
                 TGFB1 
                 7040 
               
               
                 41 
                 Hs.110443 
               
               
                 42 
                 Hs.110950 
               
               
                 43 
                 Hs.110964 
               
               
                 44 
                 Hs.111039 
                 160993 
                 NMT1 
                 4836 
               
               
                 45 
                 Hs.11114 
                   
                 DJ1181N3 
                 58476 
               
               
                 46 
                 Hs.111611 
                   
                 RPL27 
                 6155 
                 intracellular, ribosome 
               
               
                 47 
                 Hs.111779 
                 182120 
                 SPARC 
                 6678 
                 basement membrane 
               
               
                 48 
                 Hs.111779 
                 182120 
                 SPARC 
                 6678 
                 basement membrane 
               
               
                 49 
                 Hs.111779 
                 182120 
                 SPARC 
                 6678 
                 basement membrane 
               
               
                 50 
                 Hs.111779 
                 182120 
                 SPARC 
                 6678 
                 basement membrane 
               
               
                 51 
                 Hs.111988 
               
               
                 52 
                 Hs.112238 
               
               
                 53 
                 Hs.112844 
               
               
                 54 
                 Hs.11669 
                 601033 
                 LAMA5 
                 3911 
                 basement lamina 
               
               
                 55 
                 Hs.118126 
                 256540 
                 PPGB 
                 5476 
                 endoplasmic reticulum, lysosome 
               
               
                 56 
                 Hs.118893 
                 600134 
                 D2S448 
                 7837 
                 cellular_component unknown 
               
               
                 57 
                 Hs.118893 
                 600134 
                 D2S448 
                 7837 
                 cellular_component unknown 
               
               
                 58 
                 Hs.119120 
                 605568 
                 SMURF1 
                 57154 
                 intracellular 
               
               
                 59 
                 Hs.119129 
                 120130 
                 COL4A1 
                 1282 
                 collagen 
               
               
                 60 
                 Hs.119129 
                 120130 
                 COL4A1 
                 1282 
                 collagen 
               
               
                 61 
                 Hs.119129 
                 120130 
                 COL4A1 
                 1282 
                 collagen 
               
               
                 62 
                 Hs.119206 
                 602867 
                 IGFBP7 
                 3490 
                 extracellular 
               
               
                 63 
                 Hs.124 
               
               
                 64 
                 Hs.125359 
                 188230 
                 THY1 
                 7070 
                 integral plasma membrane protein 
               
               
                 65 
                 Hs.127824 
               
               
                 66 
                 Hs.13011 
                   
                 GTPBP2 
                 54676 
               
               
                 67 
                 Hs.13350 
               
               
                 68 
                 Hs.136164 
               
               
                 69 
                 Hs.143897 
                 603009 
                 DYSF 
                 8291 
                 plasma membrane 
               
               
                 70 
                 Hs.149098 
                 602127 
                 SMTN 
                 6525 
                 actin cytoskeleton 
               
               
                 71 
                 Hs.149609 
                 135620 
                 ITGA5 
                 3678 
                 cytoskeleton, extracellular matrix, 
               
               
                 72 
                 Hs.150580 
                   
                 SUI1 
                 10209 
                 cellular_component unknown 
               
               
                 73 
                 Hs.15165 
               
               
                 74 
                 Hs.151738 
                 120361 
                 MMP9 
                 4318 
                 extracellular matrix, extracellular space 
               
               
                 75 
                 Hs.155048 
                 111200 
                 LU 
                 4059 
                 integral plasma membrane protein, 
               
               
                 76 
                 Hs.155223 
                 603665 
                 STC2 
                 8614 
               
               
                 77 
                 Hs.155396 
                 600492 
                 NFE2L2 
                 4780 
                 nucleus 
               
               
                 78 
                 Hs.155894 
                 176885 
                 PTPN1 
                 5770 
                 cytoplasm, soluble fraction 
               
               
                 79 
                 Hs.158237 
                 604042 
                 ITGA10 
                 8515 
                 cytoskeleton, extracellular matrix, 
               
               
                 80 
                 Hs.159263 
                 120240 
                 COL6A2 
                 1292 
                 extracellular matrix 
               
               
                 81 
                 Hs.16007 
               
               
                 82 
                 Hs.160958 
                 605065 
                 CDC37 
                 11140 
               
               
                 83 
                 Hs.16450 
               
               
                 84 
                 Hs.172813 
                 605477 
                 P85SPR 
                 8874 
               
               
                 85 
                 Hs.173724 
                 123280 
                 CKB 
                 1152 
                 cytoplasm 
               
               
                 86 
                 Hs.173739 
               
               
                 87 
                 Hs.177596 
               
               
                 88 
                 Hs.17839 
                   
                 GG2 
                 25816 
               
               
                 89 
                 Hs.180338 
                 603366 
                 TNFRSF12 
                 8718 
                 integral plasma membrane protein, 
               
               
                 90 
                 Hs.180370 
                 661442 
                 CFL1 
                 1072 
                 cytoskeleton, nucleus 
               
               
                 91 
                 Hs.180610 
                 605199 
                 SFPQ 
                 6421 
                 nucleus 
               
               
                 92 
                 Hs.180610 
                 605199 
                 SFPQ 
                 6421 
                 nucleus 
               
               
                 93 
                 Hs.18063 
                 164720 
                 ETS1 
                 2113 
                 nucleus 
               
               
                 94 
                 Hs.18069 
                 602620 
                 PRSC1 
                 5641 
               
               
                 95 
                 Hs.180842 
                 113703 
                 RPL13 
                 6137 
                 cytosolic ribosome, intracellular 
               
               
                 96 
                 Hs.182626 
               
               
                 97 
                 Hs.184669 
                 600346 
                 ZNF144 
                 7703 
                 nucleus 
               
               
                 98 
                 Hs.185973 
                   
                 DEGS 
                 8560 
                 endoplasmic reticulum, integral plasma 
               
               
                 99 
                 Hs.193053 
                 606229 
                 EIF2C2 
                 27161 
                 cellular_component unknown 
               
               
                 100 
                 Hs.19347 
                   
                 MRPL45 
                 84311 
                 mitochondrion 
               
               
                 101 
                 Hs.19555 
               
               
                 102 
                 Hs.19561 
                 602139 
                 NDUFA7 
                 4701 
                 membrane fraction, mitochondrion, 
               
               
                 103 
                 Hs.195727 
                 606064 
                 TEM1 
                 57124 
                 extracellular matrix 
               
               
                 104 
                 Hs.197298 
                   
                 NS1 
                 10625 
                 spliceosome, transcription factor 
               
               
                 105 
                 Hs.2017 
                 604182 
                 RPL38 
                 6169 
                 60S ribosomal subunit, intracellular, 
               
               
                 106 
                 Hs.20225 
               
               
                 107 
                 Hs.205736 
                 605660 
                 HKE2 
                 10471 
                 prefoldin 
               
               
                 108 
                 Hs.20716 
                 605057 
                 TIM17 
                 10440 
                 integral plasma membrane protein, 
               
               
                 109 
                 Hs.209061 
                 603579 
                 SUDD 
                 8780 
               
               
                 110 
                 Hs.211573 
                 142461 
                 HSPG2 
                 3339 
                 basement membrane, extracellular 
               
               
                 111 
                 Hs.211612 
                   
                 SEC24A 
                 10802 
                 COPII vesicle coat, endoplasmic 
               
               
                 112 
                 Hs.211914 
                 601825 
                 NDUFS7 
                 4727 
                 mitochondrion, NADH dehydrogenase 
               
               
                 113 
                 Hs.21595 
                 312095 
                 DXYS155E 
                 8227 
                 cellular_component unknown 
               
               
                 114 
                 Hs.217493 
                 151740 
                 ANXA2 
                 302 
                 plasma membrane, soluble fraction 
               
               
                 115 
                 Hs.21906 
               
               
                 116 
                 Hs.22129 
               
               
                 117 
                 Hs.2258 
                 185260 
                 MMP10 
                 4319 
                 extracellular matrix, extracellular space 
               
               
                 118 
                 Hs.227835 
               
               
                 119 
                 Hs.23016 
                   
                 RDC1 
                 57007 
                 integral membrane protein, membrane 
               
               
                 120 
                 Hs.233955 
               
               
                 121 
                 Hs.2399 
                 600754 
                 MMP14 
                 4323 
                 extracellular matrix, integral plasma 
               
               
                 122 
                 Hs.25132 
               
               
                 123 
                 Hs.25450 
                 602193 
                 SLC29A1 
                 2030 
                 integral plasma membrane protein, 
               
               
                 124 
                 Hs.25590 
                 601185 
                 STC1 
                 6781 
               
               
                 125 
                 Hs.25590 
                 601185 
                 STC1 
                 6781 
               
               
                 126 
                 Hs.25590 
                 601185 
                 STC1 
                 6781 
               
               
                 127 
                 Hs.25664 
                   
                 DOC 
                 10263 
               
               
                 128 
                 Hs.25664 
                   
                 DOC 
                 10263 
               
               
                 129 
                 Hs.268571 
                 107710 
                 APOC1 
                 341 
               
               
                 130 
                 Hs.2706 
                 138322 
                 GPX4 
                 2879 
                 mitochondrion 
               
               
                 131 
                 Hs.272106 
               
               
                 132 
                 Hs.274184 
                 314310 
                 TFE3 
                 7030 
                 nucleus 
               
               
                 133 
                 Hs.274453 
               
               
                 134 
                 Hs.27836 
               
               
                 135 
                 Hs.278573 
                 107271 
                 CD59 
                 966 
                 membrane fraction, plasma membrane 
               
               
                 136 
                 Hs.278941 
               
               
                 137 
                 Hs.279869 
                 604853 
                 MAAT1 
                 10573 
               
               
                 138 
                 Hs.283636 
               
               
                 139 
                 Hs.284284 
               
               
                 140 
                 Hs.285814 
               
               
                 141 
                 Hs.285814 
               
               
                 142 
                 Hs.287830 
               
               
                 143 
                 Hs.289092 
                   
                 CLP 
                 23406 
                 intracellular 
               
               
                 144 
                 Hs.29288 
               
               
                 145 
                 Hs.296234 
               
               
                 146 
                 Hs.296406 
               
               
                 147 
                 Hs.29716 
               
               
                 148 
                 Hs.29797 
                 312173 
                 RPL10 
                 6134 
                 60S ribosomal subunit, intracellular, 
               
               
                 149 
                 Hs.298262 
                 603474 
                 RPS19 
                 6223 
                 40S ribosomal subunit, intracellular, 
               
               
                   
                   
                   
                   
                   
                 ribosome 
               
               
                 150 
                 Hs.299257 
               
               
                 151 
                 Hs.300954 
               
               
                 152 
                 Hs.302741 
               
               
                 153 
                 Hs.311780 
               
               
                 154 
                 Hs.312191 
               
               
                 155 
                 Hs.326445 
                 164731 
                 AKT2 
                 208 
               
               
                 156 
                 Hs.327884 
               
               
                 157 
                 Hs.332173 
                 601041 
                 TLE2 
                 7089 
                 nucleus 
               
               
                 158 
                 Hs.334604 
                   
                 KIAA1870 
                 85301 
                 collagen 
               
               
                 159 
                 Hs.334895 
                   
                 RPL10A 
                 4736 
                 60S ribosomal subunit, intracellular, 
               
               
                 160 
                 Hs.342389 
                 123840 
                 PPIA 
                 5478 
                 cytoplasm 
               
               
                 161 
                 Hs.344224 
               
               
                 162 
                 Hs.34516 
               
               
                 163 
                 Hs.347297 
               
               
                 164 
                 Hs.348428 
               
               
                 165 
                 Hs.348967 
               
               
                 166 
                 Hs.350065 
               
               
                 167 
                 Hs.351706 
               
               
                 168 
                 Hs.36353 
               
               
                 169 
                 Hs.39619 
                   
                 LOC57333 
                 57333 
               
               
                 170 
                 Hs.39871 
                 606539 
                 MYO1D 
                 4642 
                 myosin 
               
               
                 171 
                 Hs.3989 
                 604293 
                 PLXNB2 
                 23654 
                 membrane 
               
               
                 172 
                 Hs.4082 
                 606099 
                 LGALS8 
                 3964 
                 extracellular space 
               
               
                 173 
                 Hs.48320 
                   
                 DORFIN 
                 25897 
                 centrosome 
               
               
                 174 
                 Hs.48604 
               
               
                 175 
                 Hs.4980 
                 603450 
                 LDB2 
                 9079 
                 nucleus 
               
               
                 176 
                 Hs.5215 
                 602912 
                 ITGB4BP 
                 3692 
                 extrinsic plasma membrane protein, 
               
               
                 177 
                 Hs.5307 
               
               
                 178 
                 Hs.54828 
               
               
                 179 
                 Hs.56205 
                 602055 
                 INSIG1 
                 3638 
               
               
                 180 
                 Hs.57735 
                   
                 SREC 
                 8578 
                 membrane 
               
               
                 181 
                 Hs.59544 
                 126380 
                 ERCC1 
                 2067 
                 nucleus 
               
               
                 182 
                 Hs.61478 
               
               
                 183 
                 Hs.61490 
               
               
                 184 
                 Hs.6163 
                   
                 PINK1 
                 65018 
               
               
                 185 
                 Hs.61638 
               
               
                 186 
                 Hs.61661 
               
               
                 187 
                 Hs.6831 
               
               
                 188 
                 Hs.6891 
                 601944 
                 SFRS6 
                 6431 
                 nucleus 
               
               
                 189 
                 Hs.69954 
                 604349 
                 LAMC3 
                 10319 
                 extracellular matrix, membrane 
               
               
                 190 
                 Hs.70327 
                 601183 
                 CRIP2 
                 1397 
               
               
                 191 
                 Hs.70830 
                   
                 LOC51690 
                 51690 
                 nucleus, small nucleolar 
               
               
                 192 
                 Hs.7187 
               
               
                 193 
                 Hs.7247 
               
               
                 194 
                 Hs.73798 
                 153620 
                 MIF 
                 4282 
                 extracellular space 
               
               
                 195 
                 Hs.73818 
                   
                 UQCRH 
                 7388 
                 mitochondrial electron transport chain 
               
               
                 196 
                 Hs.74471 
                 121014 
                 GJA1 
                 2697 
                 connexon, integral plasma membrane 
               
               
                 197 
                 Hs.74566 
                 601168 
                 DPYSL3 
                 1809 
               
               
                 198 
                 Hs.74602 
                 107776 
                 AQP1 
                 358 
                 integral plasma membrane protein, 
               
               
                 199 
                 Hs.7486 
               
               
                 200 
                 Hs.75061 
                   
                 MLP 
                 65108 
               
               
                 201 
                 Hs.75093 
                 153454 
                 PLOD 
                 5351 
                 endoplasmic reticulum 
               
               
                 202 
                 Hs.75111 
                 602194 
                 PRSS11 
                 5654 
                 extracellular space 
               
               
                 203 
                 Hs.75616 
               
               
                 204 
                 Hs.75617 
                 120090 
                 COL4A2 
                 1284 
                 collagen, collagen type IV 
               
               
                 205 
                 Hs.75721 
                 176610 
                 PFN1 
                 5216 
                 actin cytoskeleton 
               
               
                 206 
                 Hs.75736 
                 107740 
                 APOD 
                 347 
                 extracellular space 
               
               
                 207 
                 Hs.76873 
                 603551 
                 HYAL2 
                 8692 
                 lysosome 
               
               
                 208 
                 Hs.7718 
               
               
                 209 
                 Hs.77266 
                 603120 
                 QSCN6 
                 5768 
               
               
                 210 
                 Hs.77273 
                 165390 
                 ARHA 
                 387 
                 cytoskeleton 
               
               
                 211 
                 Hs.77273 
                 165390 
                 ARHA 
                 387 
                 cytoskeleton 
               
               
                 212 
                 Hs.77274 
                 191840 
                 PLAU 
                 5328 
                 extracellular space 
               
               
                 213 
                 Hs.77326 
                 146732 
                 IGFBP3 
                 3486 
                 extracellular space 
               
               
                 214 
                 Hs.77573 
                 191730 
                 UP 
                 7378 
               
               
                 215 
                 Hs.77864 
               
               
                 216 
                 Hs.77873 
                 605715 
                 B7 
                 80381 
                 cellular_component unknown 
               
               
                 217 
                 Hs.77886 
                 150330 
                 LMNA 
                 4000 
                 lamin, nuclear lamina, nucleus 
               
               
                 218 
                 Hs.77886 
                 150330 
                 LMNA 
                 4000 
                 lamin, nuclear lamina, nucleus 
               
               
                 219 
                 Hs.77886 
                 150330 
                 LMNA 
                 4000 
                 lamin, nuclear lamina, nucleus 
               
               
                 220 
                 Hs.78281 
                 602512 
                 RGS12 
                 6002 
                 extrinsic plasma membrane protein, 
               
               
                 221 
                 Hs.78466 
                   
                 PSMD8 
                 5714 
                 19S proteasome regulatory particle 
               
               
                 222 
                 Hs.78531 
               
               
                 223 
                 Hs.78575 
                 176801 
                 PSAP 
                 5660 
                 extracellular space, integral membrane 
               
               
                 224 
                 Hs.78672 
                 600133 
                 LAMA4 
                 3910 
                 basement lamina 
               
               
                 225 
                 Hs.78869 
                 601425 
                 TCEA1 
                 6917 
                 nucleus 
               
               
                 226 
                 Hs.79339 
                 600626 
                 LGALS3BP 
                 3959 
                 extracellular space, membrane 
               
               
                 227 
                 Hs.80617 
                 603675 
                 RPS16 
                 6217 
                 40S ribosomal subunit, intracellular, 
               
               
                 228 
                 Hs.81994 
                 110750 
                 GYPC 
                 2995 
                 integral plasma membrane protein, 
               
               
                 229 
                 Hs.82002 
                 131244 
                 EDNRB 
                 1910 
                 integral plasma membrane protein, 
               
               
                 230 
                 Hs.82085 
                 173360 
                 SERPINE1 
                 5054 
               
               
                 231 
                 Hs.821 
                 301870 
                 BGN 
                 633 
                 extracellular matrix 
               
               
                 232 
                 Hs.82575 
                 603520 
                 SNRPB2 
                 6629 
                 nucleus, snRNP U2e 
               
               
                 233 
                 Hs.82749 
                 300096 
                 TM4SF2 
                 7102 
                 integral plasma membrane protein, 
               
               
                 234 
                 Hs.83126 
                 600772 
                 TAF2I 
                 6882 
                 nucleus, TFIID complex 
               
               
                 235 
                 Hs.83354 
                   
                 LOXL2 
                 4017 
                 extracellular space, membrane 
               
               
                 236 
                 Hs.83484 
                 184430 
                 SOX4 
                 6659 
                 nucleus 
               
               
                 237 
                 Hs.83484 
               
               
                 238 
                 Hs.83484 
                 184430 
                 SOX4 
                 6659 
                 nucleus 
               
               
                 239 
                 Hs.83583 
                 604224 
                 ARPC2 
                 10109 
                 actin cytoskeleton, Arp2/3 protein 
               
               
                 240 
                 Hs.84063 
               
               
                 241 
                 Hs.84753 
               
               
                 242 
                 Hs.86131 
                 602457 
                 FADD 
                 8772 
                 cytoplasm 
               
               
                 243 
                 Hs.89449 
                 600050 
                 MAP3K11 
                 4296 
               
               
                 244 
                 Hs.89640 
                 600221 
                 TEK 
                 7010 
                 integral plasma membrane protein, 
               
               
                 245 
                 Hs.89695 
                 147670 
                 INSR 
                 3643 
                 integral plasma membrane protein, 
               
               
                 246 
                 Hs.90107 
                   
                 GP110 
                 11047 
                 integral plasma membrane protein, 
               
               
                 247 
                 Hs.9096 
               
               
                 248 
                 Hs.91143 
                 601920 
                 JAG1 
                 182 
                 membrane 
               
               
                 249 
                 Hs.92236 
                   
                 KIAA0304 
                 9757 
                 nucleus 
               
               
                 250 
                 Hs.93728 
                 176311 
                 PBX2 
                 5089 
                 nucleus 
               
               
                 251 
                 Hs.9408 
               
               
                 252 
                 Hs.96908 
                   
                 PIG11 
                 9537 
               
               
                 253 
                 Hs.97199 
                 120577 
                 C1QR 
                 22918 
                 integral plasma membrane protein, 
               
               
                 254 
                 Hs.97199 
                 120577 
                 C1QR 
                 22918 
                 integral plasma membrane protein, 
               
               
                 255 
                 Hs.99093 
               
               
                   
               
             
          
         
       
     
         [0015]    Isolated and purified nucleic acids, according to the present invention are those which are not linked to those genes to which they are linked in the human genome. Moreover, they are not present in a mixture such as a library containing a multitude of distinct sequences from distinct genes. They may be, however, linked to other genes such as vector sequences or sequences of other genes to which they are not naturally adjacent. Tags disclosed herein, because of the way that they were made, represent sequences which are 3′ of the 3′ most restriction enzyme recognition site for the tagging enzyme used to generate the SAGE tags. In this case, the tags are 3′ of the most 3′ most NlaIII site in the cDNA molecules corresponding to mRNA. Nucleic acids corresponding to tags may be RNA, cDNA, or genomic DNA, for example. Such corresponding nucleic acids can be determined by comparison to sequence databases to determine sequence identities. Sequence comparisons can be done using any available technique, such as BLAST, available from the National Library of Medicine, National Center for Biotechnology Information. Tags can also be used as hybridization probes to libraries of genomic or cDNA to identify the genes from which they derive. Thus, using sequence comparisons or cloning, or combinations of these methods, one skilled in the art can obtain full-length nucleic acid sequences. Genes corresponding to tags will contain the sequence of the tag at the 3′ end of the coding sequence or of the 3′ untranslated region (UTR), 3′ of the 3′ most recognition site in the cDNA for the restriction endonuclease which was used to make the tags. The nucleic acids may represent either the sense or the anti-sense strand. Nucleic acids and proteins although disclosed herein with sequence particularity, may be derived from a single individual. Allelic variants which occur in the population of humans are included within the scope of such nucleic acids and proteins. Those of skill in the art are well able to identify allelic variants as being the same gene or protein. Given a nucleic acid, one of ordinary skill in the art can readily determine an open reading frame present, and consequently the sequence of a polypeptide encoded by the open reading frame and, using techniques well known in the art, express such protein in a suitable host. Proteins comprising such polypeptides can be the naturally occurring proteins, fusion proteins comprising exogenous sequences from other genes from humans or other species, epitope tagged polypeptides, etc. Isolated and purified proteins are not in a cell, and are separated from the normal cellular constituents, such as nucleic acids, lipids, etc. Typically the protein is purified to such an extent that it comprises the predominant species of protein in the composition, such as greater than 50, 60 70, 80, 90, or even 95% of the proteins present. 
         [0016]    Using the proteins according to the invention, one of ordinary skill in the art can readily generate antibodies which specifically bind to the proteins. Such antibodies can be monoclonal or polyclonal. They can be chimeric, humanized, or totally human. Any functional fragment or derivative of an antibody can be used including Fab, Fab′, Fab2, Fab′2, and single chain variable regions. So long as the fragment or derivative retains specificity of binding for the endothelial marker protein it can be used. Antibodies can be tested for specificity of binding by comparing binding to appropriate antigen to binding to irrelevant antigen or antigen mixture under a given set of conditions. If the antibody binds to the appropriate antigen at least 2, 5, 7, and preferably 10 times more than to irrelevant antigen or antigen mixture then it is considered to be specific. 
         [0017]    Techniques for making such partially to fully human antibodies are known in the art and any such techniques can be used. According to one particularly preferred embodiment, fully human antibody sequences are made in a transgenic mouse which has been engineered to express human heavy and light chain antibody genes. Multiple strains of such transgenic mice have been made which can produce different classes of antibodies. B cells from transgenic mice which are producing a desirable antibody can be fused to make hybridoma cell lines for continuous production of the desired antibody. See for example, Nina D. Russel, Jose R. F. Corvalan, Michael L. Gallo, C. Geoffrey Davis, Liise-Anne Pirofski. Production of Protective Human Antipneumococcal Antibodies by Transgenic Mice with Human Immunoglobulin Loci  Infection and Immunity  April 2000, p. 1820-1826; Michael L. Gallo, Vladimir E. Ivanov, Aya Jakobovits, and C. Geoffrey Davis. The human immunoglobulin loci introduced into mice: V (D) and J gene segment usage similar to that of adult humans  European Journal of Immunology  30: 534-540, 2000; Larry L. Green. Antibody engineering via genetic engineering of the mouse: XenoMouse strains are a vehicle for the facile generation of therapeutic human monoclonal antibodies  Journal of Immunological Methods  231 11-23, 1999; Yang X-D, Corvalan J R F, Wang P, Roy CM-N and Davis C G. Fully Human Anti-interleukin-8 Monoclonal Antibodies: Potential Therapeutics for the Treatment of Inflammatory Disease States.  Journal of Leukocyte Biology  Vol. 66, pp 401-410 (1999); Yang X-D, Jia X-C, Corvalan J R F, Wang P, C G Davis and Jakobovits A. Eradication of Established Tumors by a Fully Human Monoclonal Antibody to the Epidermal Growth Factor Receptor without Concomitant Chemotherapy.  Cancer Research  Vol. 59, Number 6, pp 1236-1243 (1999); Jakobovits A. Production and selection of antigen-specific fully human monoclonal antibodies from mice engineered with human Ig loci.  Advanced Drug Delivery Reviews  Vol. 31, pp: 33-42 (1998); Green L and Jakobovits A. Regulation of B cell development by variable gene complexity in mice reconstituted with human immunoglobulin yeast artificial chromosomes.  J. Exp. Med . Vol. 188, Number 3, pp: 483-495 (1998); Jakobovits A. The long-awaited magic bullets: therapeutic human monoclonal antibodies from transgenic mice.  Exp. Opin. Invest. Drugs  Vol. 7(4), pp: 607-614 (1998); Tsuda H, Maynard-Currie K, Reid L, Yoshida T, Edamura K, Maeda N, Smithies O, Jakobovits A. Inactivation of Mouse HPRT locus by a 203-bp retrotransposon insertion and a 55-kb gene-targeted deletion: establishment of new HPRT-Deficient mouse embryonic sGEM cell lines.  Genomics  Vol. 42, pp: 413-421 (1997); Sherman-Gold, R. Monoclonal Antibodies: The Evolution from &#39;80s Magic Bullets To Mature, Mainstream Applications as Clinical Therapeutics.  Genetic Engineering  News Vol. 17, Number 14 (August 1997); Mendez M, Green L, Corvalan J, Jia X-C, Maynard-Currie C, Yang X-d, Gallo M, Louie D, Lee D, Erickson K, Luna J, Roy C, Abderrahim H, Kirschenbaum F, Noguchi M, Smith D, Fukushima A, Hales J, Finer M, Davis C, Zsebo K, Jakobovits A. Functional transplant of megabase human immunoglobulin loci recapitulates human antibody response in mice.  Nature Genetics  Vol. 15, pp: 146-156 (1997); Jakobovits A. Mice engineered with human immunoglobulin YACs: A new technology for production of fully human antibodies for autoimmunity therapy.  Weir&#39;s Handbook of Experimental Immunology, The Integrated Immune System  Vol. IV, pp: 194.1-194.7 (1996); Jakobovits A. Production of fully human antibodies by transgenic mice.  Current Opinion in Biotechnology  Vol. 6, No. 5, pp: 561-566 (1995); Mendez M, Abderrahim H, Noguchi M, David N, Hardy M, Green L, Tsuda H, Yoast S, Maynard-Currie C, Garza D, Gemmill R, Jakobovits A, Klapholz S. Analysis of the structural integrity of YACs comprising human immunoglobulin genes in yeast and in embryonic sGEM cells.  Genomics  Vol. 26, pp: 294-307 (1995); Jakobovits A. YAC Vectors: Humanizing the mouse genome.  Current Biology  Vol. 4, No. 8, pp: 761-763 (1994); Arbones M, Ord D, Ley K, Ratech H, Maynard-Curry K, Otten G, Capon D, Tedder T. Lymphocyte homing and leukocyte rolling and migration are impaired in L-selectin-deficient mice.  Immunity  Vol. 1, No. 4, pp: 247-260 (1994); Green L, Hardy M, Maynard-Curry K, Tsuda H, Louie D, Mendez M, Abderrahim H, Noguchi M, Smith D, Zeng Y, et. al. Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs.  Nature Genetics  Vol. 7, No. 1, pp: 13-21 (1994); Jakobovits A, Moore A, Green L, Vergara G, Maynard-Curry K, Austin H, Klapholz S. Germ-line transmission and expression of a human-derived yeast artificial chromosome.  Nature  Vol. 362, No. 6417, pp: 255-258 (1993); Jakobovits A, Vergara G, Kennedy J, Hales J, McGuinness R, Casentini-Borocz D, Brenner D, Otten G. Analysis of homozygous mutant chimeric mice: deletion of the immunoglobulin heavy-chain joining region blocks B-cell development and antibody production.  Proceedings of the National Academy of Sciences USA  Vol. 90, No. 6, pp: 2551-2555 (1993); Kucherlapati et al., U.S. Pat. No. 6,1075,181. 
         [0018]    Antibodies can also be made using phage display techniques. Such techniques can be used to isolate an initial antibody or to generate variants with altered specificity or avidity characteristics. Single chain Fv can also be used as is convenient. They can be made from vaccinated transgenic mice, if desired. Antibodies can be produced in cell culture, in phage, or in various animals, including but not limited to cows, rabbits, goats, mice, rats, hamsters, guinea pigs, sheep, dogs, cats, monkeys, chimpanzees, apes. 
         [0019]    Antibodies can be labeled with a detectable moiety such as a radioactive atom, a chromophore, a fluorophore, or the like. Such labeled antibodies can be used for diagnostic techniques, either in vivo, or in an isolated test sample. Antibodies can also be conjugated, for example, to a pharmaceutical agent, such as chemotherapeutic drug or a toxin. They can be linked to a cytokine, to a ligand, to another antibody. Suitable agents for coupling to antibodies to achieve an anti-tumor effect include cytokines, such as interleukin 2 (IL-2) and Tumor Necrosis Factor (TNF); photosensitizers, for use in photodynamic therapy, including aluminum (III) phthalocyanine tetrasulfonate, hematoporphyrin, and phthalocyanine; radionuclides, such as iodine-131 ( 131 I), yttrium-90 ( 90 Y), bismuth-212 ( 212 Bi), bismuth-213 ( 213 Bi), technetium-99m ( 99m Tc), rhenium-186 ( 186 Re), and rhenium-188 ( 188 Re); antibiotics, such as doxorubicin, adriamycin, daunorubicin, methotrexate, daunomycin, neocarzinostatin, and carboplatin; bacterial, plant, and other toxins, such as diphtheria toxin, pseudomonas exotoxin A, staphylococcal enterotoxin A, abrin-A toxin, ricin A (deglycosylated ricin A and native ricin A), TGF-alpha toxin, cytotoxin from chinese cobra (naja naja atra), and gelonin (a plant toxin); ribosome inactivating proteins from plants, bacteria and fungi, such as restrictocin (a ribosome inactivating protein produced by  Aspergillus restrictus ), saporin (a ribosome inactivating protein from  Saponaria officinalis ), and RNase; tyrosine kinase inhibitors; ly207702 (a difluorinated purine nucleoside); liposomes containing antitumor agents (e.g., antisense oligonucleotides, plasmids which encode for toxins, methotrexate, etc.); and other antibodies or antibody fragments, such as F(ab). 
         [0020]    Those of skill in the art will readily understand and be able to make such antibody derivatives, as they are well known in the art. The antibodies may be cytotoxic on their own, or they may be used to deliver cytotoxic agents to particular locations in the body. The antibodies can be administered to individuals in need thereof as a form of passive immunization. 
         [0021]    Characterization of extracellular regions for the cell surface and secreted proteins from the protein sequence is based on the prediction of signal sequence, transmembrane domains and functional domains. Antibodies are preferably specifically immunoreactive with membrane associated proteins, particularly to extracellular domains of such proteins or to secreted proteins. Such targets are readily accessible to antibodies, which typically do not have access to the interior of cells or nuclei. However, in some applications, antibodies directed to intracellular proteins may be useful as well. Moreover, for diagnostic purposes, an intracellular protein may be an equally good target since cell lysates may be used rather than a whole cell assay. 
         [0022]    Computer programs can be used to identify extracellular domains of proteins whose sequences are known. Such programs include SMART software (Schultz et al., Proc. Natl. Acad. Sci. USA 95: 5857-5864, 1998) and Pfam software (BaGEMan et al., Nucleic acids Res. 28: 263-266, 2000) as well as PSORTII. Typically such programs identify transmembrane domains; the extracellular domains are identified as immediately adjacent to the transmembrane domains. Prediction of extracellular regions and the signal cleavage sites are only approximate. It may have a margin of error + or −5 residues. Signal sequence can be predicted using three different methods (Nielsen et al,  Protein Engineering  10:1-6, 1997, Jagla et. al, Bioinformatics 16: 245-250, 2000, Nakai, K. and Horton, P. Trends in Biochem. Sci. 24:34-35, 1999) for greater accuracy. Similarly transmembrane (TM) domains can be identified by multiple prediction methods. (Pasquier, et. al, Protein Eng. 12:381-385, 1999, Sonnhammer et al., In Proc. of Sixth Int. Conf. on Intelligent Systems for Molecular Biology, p. 175-182, Ed J. Glasgow, T. Littlejohn, F. Major, R. Lathrop, D. Sankoff, and C. Sensen Menlo Park, Calif.: AAAI Press, 1998, Klein, et. al, Biochim. Biophys. Acta, 815:468, 1985, Nakai and Kanehisa Genomics, 14: 897-911, 1992). In ambiguous cases, locations of functional domains in well characterized proteins are used as a guide to assign a cellular localization. 
         [0023]    Putative functions or functional domains of novel proteins can be inferred from homologous regions in the database identified by BLAST searches (Altschul et. al. Nucleic Acid Res. 25: 3389-3402, 1997) and/or from a conserved domain database such as Pfam (BaGEMan et.al, Nucleic Acids Res. 27:260-262 1999) BLOCKS (Henikoff, et. al, Nucl. Acids Res. 28:228-230, 2000) and SMART (Ponting, et. al, Nucleic Acid Res. 27, 229-232, 1999). Extracellular domains include regions adjacent to a transmembrane domain in a single transmembrane domain protein (out-in or type I class). For multiple transmembrane domains proteins, the extracellular domain also includes those regions between two adjacent transmembrane domains (in-out and out-in). For type II transmembrane domain proteins, for which the N-terminal region is cytoplasmic, regions following the transmembrane domain is generally extracellular. Secreted proteins on the other hand do not have a transmembrane domain and hence the whole protein is considered as extracellular. 
         [0024]    Membrane associated proteins can be engineered to delete the transmembrane domains, thus leaving the extracellular portions which can bind to ligands. Such soluble forms of transmembrane receptor proteins can be used to compete with natural forms for binding to ligand. Thus such soluble forms act as inhibitors. and can be used therapeutically as anti-angiogenic agents, as diagnostic tools for the quantification of natural ligands, and in assays for the identification of small molecules which modulate or mimic the activity of a GEM:ligand complex. 
         [0025]    Alternatively, the endothelial markers themselves can be used as vaccines to raise an immune response in the vaccinated animal or human. For such uses, a protein, or immunogenic fragment of such protein, corresponding to the intracellular, extracellular or secreted GEM of interest is administered to a subject. The immogenic agent may be provided as a purified preparation or in an appropriately expressing cell. The administration may be direct, by the delivery of the immunogenic agent to the subject, or indirect, through the delivery of a nucleic acid encoding the immunogenic agent under conditions resulting in the expression of the immunogenic agent of interest in the subject. The GEM of interest may be delivered in an expressing cell, such as a purified population of glioma endothelial cells or a populations of fused glioma endothelial and dendritic cells. Nucleic acids encoding the GEM of interest may be delivered in a viral or non-viral delivery vector or vehicle. Non-human sequences encoding the human GEM of interest or other mammalian homolog can be used to induce the desired immunologic response in a human subject. For several of the GEMs of the present invention, mouse, rat or other ortholog sequences are described herein or can be obtained from the literature or using techniques well within the skill of the art. 
         [0026]    Endothelial cells can be identified using the markers which are disclosed herein as being endothelial cell specific. These include the human markers identified by SEQ ID NOS: 1-510. Antibodies specific for such markers can be used to identify such cells, by contacting the antibodies with a population of cells containing some endothelial cells. The presence of cross-reactive material with the antibodies identifies particular cells as endothelial. Similarly, lysates of cells can be tested for the presence of cross-reactive material. Any known format or technique for detecting cross-reactive material can be used including, immunoblots, radioimmunoassay, ELISA, immunoprecipitation, and immunohistochemistry. In addition, nucleic acid probes for these markers can also be used to identify endothelial cells. Any hybridization technique known in the art including Northern blotting, RT-PCR, microarray hybridization, and in situ hybridization can be used. 
         [0027]    One can identify glioma endothelial cells for diagnostic purposes, testing cells suspected of containing one or more GEMs. One can test both tissues and bodily fluids of a subject. For example, one can test a patient&#39;s blood for evidence of intracellular and membrane associated GEMs, as well as for secreted GEMs. Intracellular and/or membrane associated GEMs may be present in bodily fluids as the result of high levels of expression of these factors and/or through lysis of cells expressing the GEMs. 
         [0028]    Populations of various types of endothelial cells can also be made using the antibodies to endothelial markers of the invention. The antibodies can be used to purify cell populations according to any technique known in the art, including but not limited to fluorescence activated cell sorting. Such techniques permit the isolation of populations which are at least 50, 60, 70, 80, 90, 92, 94, 95, 96, 97, 98, and even 99% the type of endothelial cell desired, whether normal, tumor, or pan-endothelial. Antibodies can be used to both positively select and negatively select such populations. Preferably at least 1, 5, 10, 15, 20, or 25 of the appropriate markers are expressed by the endothelial cell population. 
         [0029]    Populations of endothelial cells made as described herein, can be used for screening drugs to identify those suitable for inhibiting the growth of tumors by virtue of inhibiting the growth of the tumor vasculature. 
         [0030]    Populations of endothelial cells made as described herein, can be used for screening candidate drugs to identify those suitable for modulating angiogenesis, such as for inhibiting the growth of tumors by virtue of inhibiting the growth of endothelial cells, such as inhibiting the growth of the tumor or other undesired vasculature, or alternatively, to promote the growth of endothelial cells and thus stimulate the growth of new or additional large vessel or microvasculature. 
         [0031]    Inhibiting the growth of endothelial cells means either regression of vasculature which is already present, or the slowing or the absence of the development of new vascularization in a treated system as compared with a control system. By stimulating the growth of endothelial cells, one can influence development of new (neovascularization) or additional vasculature development (revascularization). A variety of model screening systems are available in which to test the angiogenic and/or anti-angiogenic properties of a given candidate drug. Typical tests involve assays measuring the endothelial cell response, such as proliferation, migration, differentiation and/or intracellular interaction of a given candidate drug. By such tests, one can study the signals and effects of the test stimuli. Some common screens involve measurement of the inhibition of heparanase, endothelial tube formation on Matrigel, scratch induced motility of endothelial cells, platelet-derived growth factor driven proliferation of vascular smooth muscle cells, and the rat aortic ring assay (which provides an advantage of capillary formation rather than just one cell type). 
         [0032]    Drugs can be screened for the ability to mimic or modulate, inhibit or stimulate, growth of tumor endothelium cells and/or normal endothelial cells. Drugs can be screened for the ability to inhibit tumor endothelium growth but not normal endothelium growth or survival. Similarly, human cell populations, such as normal endothelium populations or glioma endothelial cell populations, can be contacted with test substances and the expression of glioma endothelial markers and/or normal endothelial markers determined. Test substances which decrease the expression of glioma endothelial markers (GEMs) are candidates for inhibiting angiogenesis and the growth of tumors. In cases where the activity of a GEM is known, agents can be screened for their ability to decrease or increase the activity. 
         [0033]    For those glioma endothelial markers identified as containing transmembrane regions, it is desirable to identify drug candidates capable of binding to the GEM receptors found at the cell surface. For some applications, the identification of drug candidates capable of blocking the GEM receptor from its native ligand will be desired. For some applications, the identification of a drug candidate capable of binding to the GEM receptor may be used as a means to deliver a therapeutic or diagnostic agent. For other applications, the identification of drug candidates capable of mimicking the activity of the native ligand will be desired. Thus, by manipulating the binding of a transmembrane GEM receptor:ligand complex, one may be able to promote or inhibit further development of endothelial cells and hence, vascularization. 
         [0034]    For those glioma endothelial markers identified as being secreted proteins, it is desirable to identify drug candidates capable of binding to the secreted GEM protein. For some applications, the identification of drug candidates capable of interfering with the binding of the secreted GEM it is native receptor. For other applications, the identification of drug candidates capable of mimicking the activity of the native receptor will be desired. Thus, by manipulating the binding of the secreted GEM:receptor complex, one may be able to promote or inhibit further development of endothelial cells, and hence, vascularization. 
         [0035]    Expression can be monitored according to any convenient method. Protein or mRNA can be monitored. Any technique known in the art for monitoring specific genes&#39; expression can be used, including but not limited to ELISAs, SAGE, microarray hybridization, Western blots. Changes in expression of a single marker may be used as a criterion for significant effect as a potential pro-angiogenic, anti-angiogenic or anti-tumor agent. However, it also may be desirable to screen for test substances which are able to modulate the expression of at least 5, 10, 15, or 20 of the relevant markers, such as the tumor or normal endothelial markers. Inhibition of GEM protein activity can also be used as a drug screen. Human and mouse GEMS can be used for this purpose. 
         [0036]    Test substances for screening can come from any source. They can be libraries of natural products, combinatorial chemical libraries, biological products made by recombinant libraries, etc. The source of the test substances is not critical to the invention. The present invention provides means for screening compounds and compositions which may previously have been overlooked in other screening schemes. Nucleic acids and the corresponding encoded proteins of the markers of the present invention can be used therapeutically in a variety of modes. GEMs can be used to stimulate the growth of vasculature, such as for wound healing or to circumvent a blocked vessel. The nucleic acids and encoded proteins can be administered by any means known in the art. Such methods include, using liposomes, nanospheres, viral vectors, non-viral vectors comprising polycations, etc. Suitable viral vectors include adenovirus, retroviruses, and sindbis virus. Administration modes can be any known in the art, including parenteral, intravenous, intramuscular, intraperitoneal, topical, intranasal, intrarectal, intrabronchial, etc. 
         [0037]    Specific biological antagonists of GEMs can also be used to therapeutic benefit. For example, antibodies, T cells specific for a GEM, antisense to a GEM, and ribozymes specific for a GEM can be used to restrict, inhibit, reduce, and/or diminish tumor or other abnormal or undesirable vasculature growth. Such antagonists can be administered as is known in the art for these classes of antagonists generally. Anti-angiogenic drugs and agents can be used to inhibit tumor growth, as well as to treat diabetic retinopathy, rheumatoid arthritis, psoriasis, polycystic kidney disease (PKD), and other diseases requiring angiogenesis for their pathologies. 
         [0038]    Mouse counterparts to human GEMS can be used in mouse cancer models or in cell lines or in vitro to evaluate potential anti-angiogenic or anti-tumor compounds or therapies. Their expression can be monitored as an indication of effect. Mouse GEMs can be used as antigens for raising antibodies which can be tested in mouse tumor models. Mouse GEMs with transmembrane domains are particularly preferred for this purpose. Mouse GEMs can also be used as vaccines to raise an immunological response in a human to the human ortholog. 
         [0039]    The above disclosure generally describes the present invention. All references disclosed herein are expressly incorporated by reference. A more complete understanding can be obtained by reference to the following specific examples which are provided herein for purposes of illustration only, and are not intended to limit the scope of the invention. 
       Example 1 
       [0040]    In this study we employ SAGE transcript profiling to derive the transcriptomes from normal and neoplastic brain tissue. Moreover, we have employed a new version of SAGE, long SAGE, allowing for the derivation of 21 by SAGE tags. These longer tags allow for the direct interrogation of genomic DNA, identifying unique locations of cell-specific transcription. Endothelial cells from normal brain and different stages of gliomas were expression profiled and compared to each other and to the colon endothelial cell data. Distinct sets of genes define global tumor and normal endothelial cell markers as well as defining glioma-specific endothelial markers. This expanded tumor endothelial cell database will likely provide further insights into the complex regulatory mechanisms governing tumor angiogenesis. 
       Example 2 
       [0041]    Tissue procurement and endothelial cell isolation. Five separate brain tissue samples (Table 1) were resected and immediately subjected to endothelial cell isolation with slight modifications to the protocol described previously. St Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K. E., Montgomery, E., Lal, A., Riggins, G. J., Lengauer, C., Vogelstein, B., and Kinzler, K. W. (2000). Genes expressed in human tumor endothelium. Science 289, 1197-202. 
         [0042]    Briefly, samples were surgically excised and submerged in DMEM. The samples were minced into 2 centimeter cubes and subjected to tissue digestion with a collagenase cocktail. Samples were mixed at 37° C. until dissolved. Cells were spun down and washed two times with PBS/BSA and filtered through successive nylon mesh filters of 250, 100 and 40 microns. Samples were resuspended in PBS/BSA and applied to a 30% Percoll gradient centrifuging for 15 minutes at 800 g. 5 ml off the top of the percoll gradient was diluted in 50 ml DMEM and cells pelleted, washed with PBS and resuspended in 3 ml PBS/BSA. Cells were filtered through falcon blue top filter tubes, spun down and resuspended in 1 ml PBS/BSA. 100 microliters of prewashed ant-CD45 magnetic beads (Dynal) were added and the solution allowed to gently mix for ten minutes. Bead-bound cells were discarded and the supernatant transferred to a fresh microcentrifuge tube. 10 microliters of P1H12 mAB (1:100) (Brain N1, T1, and T2 samples) or UEA-I lectin (Brain N2 and T3 samples) was added and the samples were mixed gently at 4° C. for 45 minutes. Cells were pelleted and washed 3 times in PBS/BSA and resuspended in 500 microliters PBS/BSA. Prewashed goat anti-mouse M450 dynabeads were added to each tube and allowed to mix for 15 minutes at 4° C. Bead-bound cells were washed 8 times with PBS/BSA and resuspended in a final volume of 500 microliters PBS. Cells were counted and frozen at −70° C. prior to RNA extraction. 
       Example 3 
       [0043]    RNA isolation and SAGE library generation. RNA was isolated from the selected cells and initially subjected to RT-PCR analysis to determine the relative abundance of specific, known endothelial cell markers. The microSAGE protocol St Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K. E., Montgomery, E., Lal, A., Riggins, G. J., Lengauer, C., Vogelstein, B., and Kinzler, K. W. (2000). Genes expressed in human tumor endothelium. Science 289, 1197-202 (server www, domain name sagenet.org, directory sage protocol) was used to generate high-quality longSAGE libraries employing the tagging enzyme MmeI instead of BsmFI. 21 base tags were defined by capillary sequencing using a combination of an ABI 3700 and ABI 3100. The sample descriptions and sequencing depth are shown in Table 3. 
       Example 4 
       [0044]    Data analysis. Long SAGE tags derived from the brain endothelial samples were reduced to short tags to allow for the integration of colon endothelial SAGE data. Aggregate short tags were derived from the long tags. Any short tag counts that had more than one corresponding long tag representative were summed and the counts represented as one short tag. Both sequencing errors and legitimate long tag derivatives contribute to the generation of multiple long tags. For transcript and genome mapping, differential long tags were employed. Differential gene expression was evaluated as follows: For the two normal brain samples, either the maximum or minimum value was used for determining tumor/normal and normal/tumor ratios, respectively. For the three brain tumor samples, the median value was used for the tumor/normal whereas the maximum value was used for the normal/tumor ratios. A two parameter family of beta distributions was used to assess the probability of observing two fold differences in the observed SAGE tag abundances. Chen, H., Centola, M., Altschul, S. F., and Metzger, H. (1998). Characterization of gene expression in resting and activated mast cells. J Exp Med 188, 1657-68. 
       Example 5 
       [0045]    The following provides a detailed protocol useful for isolating brain endothelial cells. All steps were done at 4° C. in cold room and in centrifuge except digestion.
   1) Take sample from operating room and submerge in known volume of DMEM+ in 50 ml conical tube to measure tumor volume by displacement. Cut off 2 small pieces of tumor on dry ice and store at ±70° C. for mRNA extraction/immunohistochemistry/in situ analysis.   2) Take sample from conical and place in small amount of DMEM+in 10 cm Tissue Culture dish in hood. Mince specimen into 2 mm cubes with sterile scalpel.   3) Transfer minced specimen to small autoclaved erlenmeyer flask and add 5× volume of digestion cocktail. Sample volumes &gt;5 ml should be split into multiple flasks.   4) Mix in bacterial shaker or in 37° C. room on rotating shaker for 45 minutes or until sample is dissolved. Titrate with 10 ml piper every 15 minutes. Once a good cell suspension is obtained, remove and transfer to 50 ml conical.   
 
         [0050]    Remainder of protocol done at 4° C.
   5) Spin down at 1500 RPM (600×g) at 4° C. for 5 minutes.   6) Wash 2× with PBS/BSA and spin down again. Pool samples.   7) Filter through Nylon Mesh (250, 100, 40 micron).   8) Spin down.   9) Resuspend n PBS/BSA at ½ the original tumor volume.   10) Apply sample in 500 ul aliquots to preformed 30% Percoll gradient (Gradients needed=volume of original sample).   11) Spin at 1750 RPM (800 g) for 15 minutes.   12) Remove top 5 ml Percoll from each tube and dilute with DMEM to 50 ml volume.   13) Pellet cells in centrifuge at 1500 RPM. Pool pelleted cells.   14) Wash 2× with PBS/BSA and resuspend in 3 ml PBS/BSA.   15) Filter through Falcon Blue Top Filter tube.   16) Spin down and resuspend in 1 ml PBS/BSA in a 1.5 ml microcentrigufe tube.   17) Add 100 μl of prewashed anti-CD45 beads (hematopoietic depletion) to solution and rotate end over end in cold room for ten minutes. [For brain tissue isolation, an additional negative selection with BerEP4 epithelial depletion is not needed]   18) Remove bead-bound cells and transfer supernatant to a fresh microcentrifuge tube. Save bead-bound sample by freezing at ±70° C. Repeat extraction to ensure complete removal of all beads.   19) Add 10 ul of P1H12 mAb (1:100) to cells and mix in cold room with end-over-end rotation for 45 minutes. [As an alternative, selection using UEA1 lectin also provides quality endothelial cell selection.]   20) Pellet cells and wash 3× with PBS/BSA.   21) Resuspend cells in 500 ul of PBS/BSA.   22) Divide sample into four 1.5 ml microcentrifuge tubes (125 ul per tube) and bring volume up to 800 ul. Add 50 ul of prewashed goat anti-mouse M450 dynabeads to each tube.   23) Rotate tubes in cold room for 15 minutes.   24) Separate with magnet and save supernatant as staining control, tumor/brain fraction.   25) Rinse 8× with PBS/BSA.   26) Pool beads into single microcentrifuge tube.   27) Resuspend final cells in 500 ul plain PBS.   28) Take 5 ul of solution and combine with 5 ul of Magic DAPI and count on hemacytometer.   29) Remove 10 k cells for staining for quality control based on hemacytometer results   30) Separate beads again and freeze remainder at −70° C. for mRNA extraction.   
 
       Example 6 
       [0077]    This example describes the preparation of SAGE tags from mRNA extracted from brain endothelial cells. The preparation is described with reference to standard SAGE tag preparation procedures as are known in the art.
       All of the template was used in the PCR SAGE ditag step. Usually we take only a small portion of our template, dilute it and perform ˜300 PCR reactions. For these libraries we used all of our material, diluted it and performed ˜1200 PCR reactions.
           During the post-amplified PCR product purification step we normally do a standard large volume phenol/chloroform extraction and remove the aqueous layer which contains the product of interest. For these libraries we used Eppendorfs Phase Lock product which creates a physical barrier between the aqueous and organic layers thereby decreasing the amount of product you leave behind. This product was used for all P/C extractions in the second half of the protocol.   Digesting the amplified PCR products with NlaIII to release the ditag of interest is usually done in one reaction. For these libraries I divided the material into thirds and performed 3 NlaIII reactions in the hopes of yielding more released ditag.   Due to the low amount of material, upon entering the concatemer and digested pZERO ligation reaction, I modified the recipe for this reaction to accommodate this. Standard reaction calls for 6 ul of concatemers, 2 ul of 5× ligase buffer, 1 ul digested pZERO vector, and 1 ul of high concentrate ligase. I modified it to 6 ul of concatemers, 2 ul of 5× ligase buffer, 0.3-0.5 ul of digested pZERO vector, 1 ul high concentrate ligase and filled the missing volume with water. My intention was to favor the concatemer to pZERO ligation reaction relative to the competing pZERO to pZERO ligation reaction.   
           Most gels during the procedure showed weak amounts of product for visualization and the concatemer gels showed no visible product via the naked eye (we cut out certain fractions regardless).       
 
       Example 7 
       [0083]    Microarray Analysis. Custom 50 nucleotide oligomer arrays were constructed containing 606 unique gene elements. The 606 genes were derived from tumor and normal induced genes from both colon and brain data (328 genes), as well as 278 genes from both literature reviews and housekeeping genes. Arrays were interrogated with Cy3 and Cy5 dye-swapped labelled aRNA samples comparing HMVECs grown on plastic, collagen, fibrin, or Matrigel. 
       Example 8 
       [0084]    In situ Hybridizations and Immunohistochemistry. In situ hybridizations for PV I, VEGFR2 and vWF were carried out as described previously (10). Co-staining of PV1 and CD31 was carried out as follows: Four 500 nucleotide riboprobe fragments specific for PV1 were transcribed and used to probe formalin fixed 5 micron tissue sections. Final detection of the bound riboprobes were delayed until after the CD31 IHC staining. After PV1 hybridization and washing, tissue sections were fixed for 20 minutes in 4% formaldehyde. After a brief rinse in TBS, antigen retrieval was carried out using DAKO target retrieval solution (DAKO, Cat#S1699) according to manufacturer&#39;s instructions. After a five minute wash in TBS, slides were digested with Proteinase K at 20 ng/ml in TBS for 20 minutes at 37T, then blocked for 20 minutes at room temperature in block (10% Goat serum/0.5% Casein/0.05% Tween-20/PBS). Slides were incubated with DAKO CD31 (Cat#M0823) at a final concentration of 1 microgram/slide in block solution, for 60 minutes at room temperature. After two 5 minute TBST (DAKO, Cat#S3306) washes at room temperature, PV1 riboprobe and CD31 antibody were detected with Streptavidin-Cy2 (Jackson ImmunoResearch, Cat#016-220-084) at 5 micrograms/slide for the PV1 riboprobe, and goat anti-mouse-Cy3 (Jackson ImmunoResearch, Cat# 115-165-146) at 2.5 micrograms/slide for CD3 1, for 60 minutes at room temperature. After three Ywashes in TBST, the slides were mounted with antifade medium containing DAPI nuclear counter-stain, cover-slipped and stored at −20′C until viewing. Single images of DAPI, Cy2 and Cy3 images were acquired separately on a Zeiss Axioplan at 40× with a Hammamatsu camera, then merged together to form a composite image using universal imaging metamorph software, and stored at −20C until viewing. 
       Example 9 
       [0085]    Capillary-like tubule formation assay. The formation of capillary-like tubular structures was assessed in Matrigel-coated multiwell plates essentially as described previously (12). Briefly, 300 microliters of Matrigel (BD, Bedford, Mass.) was added to each well of a 24 well plate and allowed to polymerize at 37′C for 30 minutes. HMVECs (BioWhittaker) were infected with adenovius harboring Tem.1 or GFP gene or empty vector (EV) for 67 hours at 300 MOI (Multiplicity Of Infection). Cells were then seeded at a density of 30×103 cells/well in 500 microliters EGM-2 medium with supplements (BioWhittaker) in Matrigel-coated plates and incubated at 37′C for 24 hours and viewed using a Nikon Eclipse TE200 microscope under a phase contrast and photographed. Images were analyzed using software Scion Image (Scion Corporation, Frederick, Md.) under the mode of integrated density. 
       Example 9 
       [0086]    Cell Proliferation Assay. HMVEC proliferation was assessed by the Cell Titer-Glo Luminescent Cell Viability Assay (Promega, Madison, Wis.) in 96-well cell culture plates. HMVECs were seeded at 2,000 cells per well in 100 microliters medium and plates were incubated at 37′C for 48 hours. Reagent was added to each well according to manufacture&#39;s instruction, and fluorescence was measured using the Millipore CytoFluor2350. 
       Example 10 
       [0087]    Five independent endothelial cell populations were purified from glioma tumor tissue and normal brain tissue. In this study, the tissue defined as normal is derived from patients with epilepsy who have undergone a temporal lobectomy. The samples are summarized in Table 3. Samples N1, T1 and T2 were ultimately P1H12-selected and samples N2 and T3 were UEA-I selected. Prior to SAGE analysis, each sample was assessed for the relative mRNA abundance for vWF, Glial fibrillary acidic protein (GFAP) and EF1 by RT-PCR. Abundant levels of vWF and the control housekeeper EF1, and low levels of the glial cell-specific gene GFAP suggested the cell population was primarily endothelial (data not shown). SAGE analysis was performed to a depth of approximately 50,000 tags (Table 3). For data analysis, each SAGE project was normalized to exactly 50,000 tags. Pairwise comparisons between expression data derived from tumor samples selected with P1H12 or UEA-I showed correlation coefficients around 80%, slightly higher than a comparison between two tumor samples both selected with P1H12. This suggests that selecting endothelial cells with either P1H12 or UEA-I results in highly similar cell populations. Moreover, nearly half of the tumor specific markers revealed in this study are induced 4 fold in each of the normal samples used, suggesting the normal samples are similar populations as well. With this in mind, we felt that combining data for the two normal samples and for the three tumor samples was appropriate. 
         [0000]                                      TABLE 3                   Samples used in this study.                    Tags           Sample   Description   Generated   EC Selection               Brain N1   Normal temporal lobectomy ECs   43,000   P1H12       Brain N2   Normal temporal lobectomy ECs   49,000   UEA-I       Brain T1   Grade IV Glioma Ecs   46,000   P1H12       Brain T2   Grade III Glioma Ecs   50,000   P1H12       Brain T3   Grade IV Glioma Ecs   58,000   UEA-I       Colon N*   Normal colon Ecs   96,000   P1H12       Colon T*   Tumor colon Ecs   96,000   P1H12       Fetal Brain   Normal bulk   204,000    —       Fetal Kidney   Normal bulk    50,000+   —                    
Genes specific for endothelial cells showed expression levels consistent with the previously examined colon endothelial SAGE data (Table 4). Additionally, markers specific for epithelial, hematopoeitic or glial cells showed limited or no expression in the brain endothelial libraries suggesting little contamination from non-endothelial cell populations (Table 4). Finally, the data generated here allow for the derivation of alt gene EC prediction class of which 6 have been previously described as EC-specific (Huminiecki, L., and Bicknell, R. (2000). In silico cloning of novel endothelial-specific genes. Genome Res 10, 1796-806.) (data not shown). This provides further evidence of pure EC populations used for this study.
 
         [0000]    
       
         
               
             
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Cell specificity markers. 
               
             
          
           
               
                 Gene 
                 Specificity 
                 Colon N 
                 Colon T 
                 Brain N1 
                 Brain N2 
                 Brain T1 
                 Brain T2 
                 Brain T3 
               
               
                   
               
             
          
           
               
                 Hevin 
                 EC 
                 161 
                 69 
                 51 
                 99 
                 223 
                 121 
                 48 
               
               
                 VWF 
                 EC 
                 35 
                 33 
                 12 
                 53 
                 37 
                 51 
                 110 
               
               
                 Tie2/Tek 
                 EC 
                 4 
                 2 
                 2 
                 4 
                 1 
                 4 
                 3 
               
               
                 CD34 
                 EC 
                 5 
                 2 
                 3 
                 10 
                 12 
                 4 
                 11 
               
               
                 CD14 
                 Hematopoeitic 
                 1 
                 1 
                 1 
                 2 
                 0 
                 0 
                 1 
               
               
                 CK8 
                 Epithelial 
                 1 
                 2 
                 0 
                 0 
                 2 
                 1 
                 1 
               
               
                 GLUT1 
                 Brain EC 
                 0 
                 1 
                 8 
                 37 
                 2 
                 25 
                 8 
               
               
                 GFAP 
                 Glial 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
                 0 
               
               
                   
               
             
          
         
       
     
         [0088]    Genes expressed preferentially in glioma derived endothelial cells as opposed to normal endothelial cells are potentially involved in regulating angiogenesis-dependent tumor growth. Specific parameters for the sorting of SAGE data and the layering of additional statistical filters allowed for a conservative estimate of legitimate differentially expressed genes (see Methods). Excluding mitochondrial genes, 131 genes were observed to be induced in the glioma endothelial cells based on a four fold induction ratio. Only 14 genes can be entertained as glioma-specific when additional statistical filters are applied (Table 5). In this case, a two fold parameter family of distributions was used to establish a 90% probability of observing at least a 2 fold difference in values. Only one of these twelve genes, apolipoprotein D, shows higher expression in the stage La glioma than at least one of the stage IV tumors. This suggests that many of the highly induced glioma endothelial genes revealed in this analysis may be involved in later stages of angiogenesis where the initiation of vascular sprouting has already occurred or are glioma type specific showing representation in the astrocytoma and not oligodendroglioma-derived ECs. Less highly induced genes, or genes primarily induced in the less aggressive tumor stage, may be more reflective of angiogenesis initiation. Several genes regulating extracellular matrix architecture are revealed as highly induced in this study. HSPG2 (perlecan), several type IV collagen transcript variants, and matrix metalloprotease 14 (MMP14) have all been shown to play a role in remodeling the extracelullar matrix. Interestingly, other genes that play roles in either cellular signaling or cell-cell communication are also highly expressed exclusively in glioma-associated endothelial cells. Melanoma associated antigen (MG50), endothelin receptor, the G-protein coupled receptor RDC-1, and integrin αV are all cell surface proteins previously demonstrated to play a role in signaling cascades. Although the endothelin receptor, RDC-1 and integrin αV have previously been shown to regulate angiogenesis, MG50 does not have an association with angiogenesis. Moreover, MG50 was previously shown to be selectively associated with several types of tumor cells with a function yet to be defined. It is noteworthy that the p53-induced, brain-specific angiogenesis inhibitor (BAI-1) was expressed to significant levels but restricted to the earlier stage tumor present in this study (data not shown). It is possible that the loss of expression of BAI-1 in the later tumor stages reflects the need to more aggressively advance vascular development. Other than the detection of a differential HEYL SAGE tag, no other colon endothelial markers were observed to be preferentially expressed in the grade III tumor. In total, of the 14 tumor induced genes listed, 12 are either present on the cell surface or secreted. The localization of the remaining two gene products has yet to be determined as these genes remain uncharacterized. Finally, it is noteworthy that only a select few genes show significant (&gt;2 tags) expression in a fetal brain library where angiogenesis is expected to be robust. 
         [0089]    In contrast to the highly biased localization of glioma-induced endothelial cell gene expression defined above, genes that are induced in the normal endothelial cells relative to glioma endothelial cells show a radically different cellular distribution. Twenty-one genes are induced 4 fold or greater in the normal endothelial cells. Filtering for genes with a 50% or greater chance of having greater than 2 fold difference in transcript abundance reduces this list to 14 genes (Table 6). Protein products predicted for these 14 genes show a range of cellular localizations with 4 gene products being intracellular, 5 being integral membrane proteins, 3 extracellular, and one each either secreted, on the cell surface or a nuclear membrane receptor. Several of these genes have functions consistent with either tumor suppressor or anti-angiogenic functions. These anti-proliferative functions have been ascribed to the early growth response gene 1 (EGR1), BTG2, Fruppel-like factor 4 (KLF4), and the serine protease inhibitor SPINT2 although associations with angiogenesis are limited to SPINT2. The down-regulation of these genes in each of the three glioma tumors suggests that these genes may function to encode proteins with anti-angiogenic properties. Both SPINT2 and BTG2 are secreted and may act via paracrine mechanisms. Also noteworthy is the preferential expression of the secreted protein MT1A as this metalothionein may serve as an antioxidant potentially attenuating DNA damage within adjacent cells. Interestingly, EGR1 and KLF4′ encode transcription factors suggesting that some part of the anti-angiogenic pathway revealed here may be initiated by these gene products. With the exception of MT1A, none of the above genes show differential expression in colon tumor ECs and may therefore be glioma-specific EC markers. 
         [0090]    The specificity of gene expression for tumor EC subtypes is important to define and can be addressed with the glioma EC data integrated with data obtained previously for colon EC populations. A limited number of genes are preferentially expressed in both brain and colon normal EC populations. In contrast, 16 genes were induced at least 4 fold in both colon and brain tumor EC fractions. 12 of these genes also met the criteria of having a greater than 50% chance of being at least 2 fold differential (Table 7). The majority of these genes (7) are collagen transcripts. However, tumor endothalial marker 1 (TEM1), THY1, and RDC-1 also show consistent induction in the different tumor EC cells. This limited conservation of tumor-induced EC expression suggests highly specific EC expression profiles dependent on the tissue source. TEM1 expression has been validated on tissue arrays harboring tissue slices from astrocytomas (data not shown). 
         [0091]    Defining the specificity of gene expression to particular cell types can assist in determining function and designing therapeutics. Our non-endothelial cell SAGE database currently contains 76 libraries encoding 255,000 unique SAGE transcripts. The epithelial cell lines derive from lung, ovary, kidney, prostate, breast, colon, pancreas. Additional non-epithelial sources include cardiomyocytes, melanocytes, glioblastoma and monocytes. Genes which show induction in glioma ECs and demonstrate a restricted expression in non-EC cells may be ideal targets for anti-angiogenic therapies. Allowing for 1 or fewer tags in any non-EC library and at least a four-fold induction in glioma ECs yielded only 5 genes (Table 8). Some of these genes are likely not EC-specific due to the relatively limited number of cell types included within the non-EC database. However, both PV-1 and Plexin A2 (PLXNA2) are interesting genes with potential functional relevance to angiogenesis regulation. 
         [0092]    The SAGE tag that defines PLXNA2 falls outside of the current mRNA boundaries residing 3′ of the ultimate exon. RT-PCR results, however, have confirmed transcription of mRNA containing this tag in the tumor samples used to derive the SAGE data. Plexins share homology with the scatter factor/hepatocyte growth factor (SF/HGF) family of receptors encoded by the MET gene family [Tamagnone, L., Artigiani, S., Chen, H., He, Z., Ming, G. I., Song, H., Chedotal, A., Winberg, M. L., Goodman, C. S., Poo, M., Tessier-Lavigne, M., and Comoglio, P. M. (1999). Plexins are a large family of receptors for transmembrane, secreted, and GPI-anchored semaphorins in vertebrates. Cell 99, 71-80.] Earlier results have demonstrated a link between SF/HGF expression and increase tumorigencity [Bowers, D. C., Fan, S., Walter, K. A., Abounader, R., Williams, J. A., Rosen, E. M., and Laterra, J. (2000). Scatter factor/hepatocyte growth factor protects against cytotoxic death in human glioblastoma via phosphatidylinositol 3-kinase- and AKT-dependent pathways. Cancer Res 60, 4277-83.] Moreover, SF/HGF promotes this increased tumorigencity with concordant stimulation in angiogenesis [Lamszus, K., Laterra, J., Westphal, M., and Rosen, E. M. (1999). Scatter factor/hepatocyte growth factor (SF/HGF) content and function in human gliomas. Int J Dev Neurosci 17, 517-30.] In vivo targeting of SF/HGF was demonstrated to inhibit glioma growth and angiogenesis [Abounader, R., Lal, B., Luddy, C., Koe, G., Davidson, B., Rosen, E. M., and Laterra, J. (2002). In vivo targeting of SF/HGF and c-met expression via U1snRNA/ribozymes inhibits glioma growth and angiogenesis and promotes apoptosis. Faseb J 16, 108-10.]. Plexins are known to function as coreceptors with neuropilin 1 functioning as a receptor for semaphorin and, in turn, regulating neuronal guidance and cell association [Tamagnone, 1999, supra]. As neuropilin-1 and Plexin association can serve to receive signals from semaphorins to guide neuronal growth, it is conceivable that a Plexin-neuropilin association may regulate angiogenic growth in a manner analogous to KDR-neuropilin complexes signaling VEGF responses. Plexin A2 shows very low level expression in colon ECs and is not differentially induced in colon tumor ECs. It is noteoworthy that another plexin, plexin B2 (PLXNB2), also showed a five fold increase in glioma EC expression but did not make the statistical threshold demanded for Table 8. Plexin B2 was previously shown to be differentially induced in brain tumors [Shinoura, N., Shamraj, O. I., Hugenholz, H., Zhu, J. G., McBlack, P., Warnick, R., Tew, J. J., Wani, M. A., and Menon, A. G. (1995). Identification and partial sequence of a cDNA that is differentially expressed in human brain tumors. Cancer Lett 89, 215-21.] The upregulation of plexins in glioma ECs allows for a hypothesis whereby SF/HGF directly stimulates EC migration and proliferation. The novel discovery of a consistently upregulated level of Plexin A2 in gliomas requires further evidence for a functional link between tumor levels of plexin A2 and angiogenesis regulation, particularly in the brain. 
         [0093]    PV-1 (also called PLVAP for plasmallema vesicle associated protein), is a recently discovered type II integral membrane glycoprotein shown to colocalize with caveolin-1. Stan, R. V., Arden, K. C., and Palade, G. E. (2001). cDNA and protein sequence, genomic organization, and analysis of cis regulatory elements of mouse and human PLVAP genes. Genomics 72, 304-13. Interestingly, this protein was the first to be shown to localize to the stomatal diaphragms and transendothelial channels within caveolae. The specific function of PV-1 remains unknown. PV-1 is expressed at substantial levels in colon ECs but is not expressed differentially between normal and tumor colon ECs. The upregulation of this caveolae-associated protein in gliomas may provide a means for specifically targeting glioma-associated endothelial cells as well as potentially providing a therapeutic delivery mechanism to the underlying tumorigenic cells (Marx, J. (2001). Caveolae: a once-elusive structure gets some respect. Science 294, 1862-5.)) 
         [0094]    From this study there is also the potential to define brain EC specific genes irrespective of function or differential expression in normal or tumor tissue. Applying the same criteria as that applied for defining EC restricted glioma induced genes, only two genes, TNFα-induced protein 3 and JUNB, show consistent expression in the brain EC samples but severely limited expression in non-EC databases. 
         [0095]    The blood brain barrier within brain capillary endothelial cells results in a restricted diffusion of both small and large molecules as compared to non-brain EC junction complexes. As a result of this, brain capillary ECs facilitate molecular exchange via a tightly regulated, or catalyzed transport system. Any differential expression of catalyzed membrane transporters between normal and tumor tissue may provide a means to selectively deliver therapies to tumor cells. The insulin receptor (IR) has been known for some time to be a marker for brain capillary ECs and to facilitate delivery of drugs. One of the most highly induced, glioma-specific genes in this study is the IR (Table 8). The high induction of IR transcripts in gliomas was not previously recognized and may provide a selective delivery mechanism to cancer cells as these receptors are also proposed to reside within caveolae structures [Smith, R. M., Jarret, L. (1988). Lab. Invest. 58, 613-629.] Overall, very few transporters showed a differential induction in glioma-associated ECs as compared to their normal counterpart (Table 9). This is counter to previous suggestions linking altered expression of transporters with histologic grade of CNS tumors [Guerin, C., Wolff, J. E., Laterra, J., Drewes, L. R., Brem, H., and Goldstein, G. W. (1992). Vascular differentiation and glucose transporter expression in rat gliomas: effects of steroids. Ann Neurol 31, 481-7.] Only one other gene, SLC1A5 Solute carrier family 1 member 5 (neutral amino acid transporter), showed a greater than 4 fold induction in glioma-derived ECs. It should be stated, however, that the standard SAGE tag for integrin αV is shared with aquaporin. Long tag derivations of these two genes revealed that both integrin αV and aquaporin are induced in glioma ECs. Aquaporin may play a role in caveolae swelling that accompanies VEGF stimulated EC growth [Roberts, W. G., and Palade, G. E. (1997). Neovasculature induced by vascular endothelial growth factor is fenestrated. Cancer Res 57, 765-72.] Only one membrane transporter, Na+/K+ transporting ATP1A2 ATPase, was reciprocally repressed in glioma-derived ECs. It remains possible that certain transporters were missed in this analysis due to incorrect functional assignment. Nonetheless, the low number of differentially regulated transport facilitators suggests a small number of these genes need to be transcriptionally activated to accommodate any necessary increase in protein abundance required for tumor growth. 
         [0096]    Table 10 shows genes induced in glioma endothelial cells but not in colon tumor or breast tumor endothelial cells. 
         [0097]    Table 11 shows genes which encode transporters which are repressed in glioma endothelial cells. 
         [0098]    Table 12 shows genes which encode proteins which are localized to the nucleus of both brain and colon tumor endothelial cells. 
         [0099]    Table 13 shows genes which encode proteins which are localized to the cytoplasm of both brain and colon tumor endothelial cells. 
         [0100]    Table 14 shows genes which encode proteins which are extracellular from both brain and colon tumor endothelial cells. 
         [0101]    Table 15 shows genes which encode proteins which are localized to the membrane of both brain and colon tumor endothelial cells. 
         [0102]    Table 16 shows genes which encode proteins which are induced in both brain and colon tumor endothelial cells. 
         [0103]    Table 17 shows additional tumor endothelial markers in brain. 
         [0104]    Table 18 shows tumor endothelial markers in the brain which are cytoplasmic. 
         [0105]    Table 19 shows tumor endothelial markers in the brain which are nuclear. 
         [0106]    Table 20 shows tumor endothelial markers in the brain which are membrane associated. 
         [0107]    Table 21 shows tumor endothelial markers in the brain which are extracellular. 
         [0108]    Table 22 shows tumor endothelial markers in the brain which are unsorted with respect to cellular localization. 
       REFERENCES 
       [0000]    
       
         Abounader, R., Lal, B., Luddy, C., Koe, G., Davidson, B., Rosen, E. M., and Laterra, J. (2002). In vivo targeting of SF/HGF and c-met expression via U1snRNA/ribozymes inhibits glioma growth and angiogenesis and promotes apoptosis. Faseb J 16, 108-10. 
         Bart, J., Groen, H. J., Hendrikse, N. H., van der Graaf, W. T., Vaalburg, W., and de Vries, E. G. (2000). The blood-brain barrier and oncology: new insights into function and modulation. Cancer Treat Rev 26, 449-62. 
         Bernsen, H. J., Rijken, P. F., Oostendorp, T., and van der Kogel, A. J. (1995). Vascularity and perfusion of human gliomas xenografted in the athymic nude mouse. Br J Cancer 71, 721-6. 
         Bowers, D. C., Fan, S., Walter, K. A., Abounader, R., Williams, J. A., Rosen, E. M., and Laterra, J. (2000). Scatter factor/hepatocyte growth factor protects against cytotoxic death in human glioblastoma via phosphatidylinositol 3-kinase- and AKT-dependent pathways. Cancer Res 60, 4277-83. 
         Chen, H., Centola, M., Altschul, S. F., and Metzger, H. (1998). Characterization of gene expression in resting and activated mast cells. J Exp Med 188, 1657-68. 
         Guerin, C., Wolff, J. E., Laterra, J., Drewes, L. R., Brem, H., and Goldstein, G. W. (1992). Vascular differentiation and glucose transporter expression in rat gliomas: effects of steroids. Ann Neurol 31, 481-7. 
         Hobbs, S. K., Monsky, W. L., Yuan, F., Roberts, W. G., Griffith, L., Torchilin, V. P., and Jain, R. K. (1998). Regulation of transport pathways in tumor vessels: role of tumor type and microenvironment. Proc Natl Acad Sci USA 95, 4607-12. 
         Holash, J., Maisonpierre, P. C., Compton, D., Boland, P., Alexander, C. R., Zagzag, D., Yancopoulos, G. D., and Wiegand, S. J. (1999). Vessel cooption, regression, and growth in tumors mediated by angiopoietins and VEGF. Science 284, 1994-8. 
         Huminiecki, L., and Bicknell, R. (2000). In silico cloning of novel endothelial-specific genes. Genome Res 10, 1796-806. 
         Lamszus, K., Laterra, J., Westphal, M., and Rosen, E. M. (1999). Scatter factor/hepatocyte growth factor (SF/HGF) content and function in human gliomas. Int J Dev Neurosci 17, 517-30. 
         Marx, J. (2001). Caveolae: a once-elusive structure gets some respect. Science 294, 1862-5. 
         Roberts, W. G., and Palade, G. E. (1997). Neovasculature induced by vascular endothelial growth factor is fenestrated. Cancer Res 57, 765-72. 
         Shinoura, N., Shamraj, O, I., Hugenholz, H., Zhu, J. G., McBlack, P., Warnick, R., Tew, J. J., Wani, M. A., and Menon, A. G. (1995). Identification and partial sequence of a cDNA that is differentially expressed in human brain tumors. Cancer Lett 89, 215-21. 
         Smith, R. M., Janet, L. (1988). Lab. Invest. 58, 613-629. 
         St Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K. E., Montgomery, E., Lal, A., Riggins, G. J., Lengauer, C., Vogelstein, B., and Kinzler, K. W. (2000). Genes expressed in human tumor endothelium. Science 289, 1197-202. 
         Stan, R. V., Arden, K. C., and Palade, G. E. (2001). cDNA and protein sequence, genomic organization, and analysis of cis regulatory elements of mouse and human PLVAP genes. Genomics 72, 304-13. 
         Tamagnone, L., Artigiani, S., Chen, H., He, Z., Ming, G. I., Song, H., Chedotal, A., Winberg, M. L., Goodman, C. S., Poo, M., Tessier-Lavigne, M., and Comoglio, P. M. (1999). Plexins are a large family of receptors for transmembrane, secreted, and GPI-anchored semaphorins in vertebrates. Cell 99, 71-80. 
         Vajkoczy, P., and Menger, M. D. (2000). Vascular microenvironment in gliomas. J Neurooncol 50, 99-108. 
         Vajkoczy, P., Schilling, L., Ullrich, A., Schmiedek, P., and Menger, M. D. (1998). Characterization of angiogenesis and microcirculation of high-grade glioma: an intravital multifluorescence microscopic approach in the athymic nude mouse. J Cereb Blood Flow Metab 18, 510-20. 
         Vick, N. A., and Bigner, D. D. (1972). Microvascular abnormalities in virally-induced canine brain tumors. Structural bases for altered blood-brain barrier function. J Neurol Sci 17, 29-39. 
       
     
         [0000]    
       
         
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 5 
               
               
                   
               
               
                 T/N 
                 T/N prob. 
                 SAGE Tag 
                 UG ID 
                 UG description 
                 localization 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 17 
                 95 
                 GTCTCAGTGC 
                 116893 
                 Melanoma associated gene MG50 
                 surface/secreted 
                   
               
               
                   
               
               
                 14 
                 90 
                 CTTATGCTGC 
                 82002 
                 endothelin receptor type B 
                 surface 
               
               
                   
               
               
                 13 
                 99 
                 CCACCCTCAC 
                 211573 
                 HSPG2 Perlecan 
                 extracellular 
               
               
                   
               
               
                 13 
                 94 
                 GTGCTACTTC 
                 119129 
                 collagen, type IV, alpha 1 
                 extracellular 
               
               
                   
               
               
                 12 
                 98 
                 GAGTGAGACC 
                 345843 
                 Thy-1 cell surface anbgen 
                 surface 
               
               
                   
               
               
                 10 
                 94 
                 ATGGCAACAG 
                 149609 
                 ITGA5 integrin alpha 5 (Fn receptor) receptor 
                 surface 
               
               
                   
               
               
                 9 
                 91 
                 TCACACAGTG 
                 23016 
                 G protein-coupled receptor RDC-1 
                 surface 
               
               
                   
               
               
                 8 
                 100 
                 GACCGCAGG 
                 119129 
                 collagen, type IV, alpha 1 
                 extracellular 
               
               
                   
               
               
                 8 
                 97 
                 GGGAGGGGTG 
                 239 
                 matrix metalloproteinase 14 (membrane-inserted) 
                 extracellular 
               
               
                   
               
               
                 7 
                 99 
                 CCCTACCCTG 
                 75736 
                 apolipoprotein D 
                 extracellular 
               
               
                   
               
               
                 8 
                 97 
                 TTCTCCCAAA 
                 75617 
                 collagen, type IV, alpha 2 
                 extracellular 
               
               
                   
               
               
                 6 
                 98 
                 GGATGCGCAG 
                 302741 
                   Homo sapiens  mRNA full length insert cDNA clone EU 
               
               
                   
               
               
                 5 
                 98 
                 GTGCTAAGCG 
                 159263 
                 collagen, type VI, alpha 2 Exon 1 
                 extracellular 
               
               
                   
               
               
                 4 
                 93 
                 CCCAGGACAC 
                 110443 
                   Homo sapiens  cDNA: FLJ22215 fis, clone HRC01580. 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 6 
               
               
                   
               
               
                 Brain 
                 Brain 
                   
                   
                   
                   
                   
               
               
                 N/T 
                 N/T prob 
                 SAGE Tag 
                 UG ID 
                 UG description 
                 Localization 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 9 
                 72 
                 TAGTTGGAAA 
                 1119 
                 nuclear receptor subfamily 4, group A, member 1 NR4A1 
                 nuclear 
                   
               
               
                   
                   
                   
                   
                   
                 membrane 
               
               
                   
               
               
                 9 
                 72 
                 AAGGGCGCGG 
                 1378 
                 annexin A3 ANXA3 
                 membrane 
               
               
                   
               
               
                 9 
                 72 
                 AGCTGTGCCA 
                 348254 
                 metallothionein 1A (functional) MT1A 
                 extracellular 
               
               
                   
               
               
                 7 
                 60 
                 ACAAAATCAA 
                 110613 
                 nuclear pore complex interacting protein SMG-1 
                 membrane 
               
               
                   
               
               
                 6 
                 68 
                 GCCTGCAGTC 
                 31439 
                 serine prolease inhibitor, Kunitz type, 2 SPINT2 
                 extracellutar 
               
               
                   
               
               
                 6 
                 52 
                 ACCAGGTCCA 
                 5167 
                 solute carrier family 5 (sodium-dependent vitamin 
                 membrane 
               
               
                   
                   
                   
                 334549 
               
               
                   
               
               
                 6 
                 52 
                 GGCTAATTAT 
                 34114 
                 ATPase. Na+/K+ transporting. alpha 2 (+) polypepti 
                 membrane 
               
               
                   
               
               
                 6 
                 75 
                 TTTAAATAGC 
                 7934 
                 KLF4 Kruppel-like factor 4 (gut) 
                 intracellular 
               
               
                   
               
               
                 5 
                 81 
                 CAGTTCATTA 
                 326035 
                 early growth response 1 EGR1 
                 intracellular 
               
               
                   
               
               
                 5 
                 61 
                 CTGCCGTGAC 
                 75462 
                 BTG family, member 2 BTG2 
                 extracellular 
               
               
                   
               
               
                 5 
                 65 
                 TTTTAACTTA 
                 160483 
                 erythrocyle membrane protein band 7.2 (stomatin) 
                 membrane 
               
               
                   
               
               
                 4 
                 77 
                 TAGAAACCGG 
                 8997 
                 heat shock 70kD protein 1A HSP70 
                 intracellular 
               
               
                   
               
               
                 4 
                 77 
                 CTTCTTGCC 
                 272572 
                 hemoglobin, alpha 2 
                 intracellular 
               
               
                   
                   
                   
                 347939 
               
               
                   
               
               
                 4 
                 53 
                 TAGAAAAAAT 
                 8906 
                 syntaxin 7 
                 surface 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 7 
               
               
                   
               
               
                 Brain 
                 colon 
                   
                   
                   
                   
                   
               
               
                 T/N 
                 T/N 
                 SAGE Tag 
                 UG ID 
                 UG description 
                 localization 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 13 
                 4 
                 GTGCTAGTTC 
                 119129 
                 collagen, type IV, alpha 1 
                 extracellular 
                   
               
               
                   
               
               
                 12 
                 16 
                 GAGTGAGACC 
                 125359 
                 Thy-1 cell surface antigen 
                 surface 
               
               
                   
               
               
                 9 
                 4 
                 TCACACAGTG 
                 23016 
                 G protein-coupled receplor RDC-1 
                 surface 
               
               
                   
               
               
                 8 
                 6 
                 GACCGCAGGA 
                 119129 
                 collagen, type IV, alpha 1 
                 extracellular 
               
               
                   
               
               
                 8 
                 13 
                 GGGAGGGGTG 
                 2399 
                 matrix metalloproteinase 14 (membrane-inserted) 
                 extracellular 
               
               
                   
               
               
                 7 
                 14 
                 GGGGCTGCCC 
                 195727 
                 tumor endothleial marker 1 precursor 
                 surface 
               
               
                   
               
               
                 6 
                 4 
                 TTCTCCCAAA 
                 75617 
                 collagen, type IV, alpha 2 
                 extracellular 
               
               
                   
               
               
                 6 
                 18 
                 CCACAGGGGA 
                 119571 
                 collagen, type III, alpha 1 (Ehlers-Danlos syndrom 
                 extracellular 
               
               
                   
               
               
                 6 
                 9 
                 TCAAGTTCAC 
                 351928 
                   Homo sapiens  mRNA full length insert cDNA Euroimage 1977059 
               
               
                   
               
               
                 5 
                 10 
                 ACCAAAAACC 
                 172928 
                 collagen, type I, alpha 1 
                 extracellular 
               
               
                   
               
               
                 4 
                 7 
                 GATCAGGCCA 
                 119571 
                 collagen, type III, alpha 1 (Ehlers-Danlos syndrom 
                 extracellular 
               
               
                   
               
               
                 4 
                 4 
                 AGAAACCACG 
                 119129 
                 collagen, type IV, alpha 1 
                 extracellular 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 8 
               
               
                   
               
               
                 Brain 
                 Brain 
                   
                 non-EC 
                   
                   
                   
                   
               
               
                 T/N 
                 T/N prob 
                 ShortTag 
                 count 
                 UG ID 
                 UG description 
                 Localization 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 9 
                 83 
                 AAGGTTCTTC 
                 1 
                 89695 
                 insulin receptor 
                 surface 
                   
               
               
                   
               
               
                 7 
                 74 
                 CCCTTTCACA 
                 1 
                 107125 
                 PV1 
                 surface 
               
               
                   
               
               
                 6 
                 75 
                 AGACTAGGGG 
                 1 
                 350065 
                 Plexin A2 
                 surface 
               
               
                   
               
               
                 4 
                 69 
                 CATAAACGGG 
                 1 
                 69954 
                 laminin, gamma 3 
                 extracellular 
               
               
                   
               
               
                 4 
                 53 
                 GGCCAACATT 
                 1 
                 36353 
                 Homo sapiens mRNA full length insert cDNA clone EU 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 9 
               
               
                   
               
               
                 Short Tag 
                 Long Tag 
                 UG ID 
                 UG Description 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 GTACGTCCCA 
                 GTACGTCCCACCCTGTC 
                 183556 
                 solute carrier family 1 (neutral amino acid transp 
                   
               
               
                   
               
               
                 GCAATTTAAC 
                 GCAATTTAACCACATTT 
                 83974 
                 solute carrier family 21 (prostaglandin transporte 
               
               
                   
               
               
                 AGGTGCGGGG 
                 AGGTGCGGGGGGCAGAC 
                 165439 
                 arsA (bacterial) arsenite transporter, ATP-binding 
               
               
                   
               
               
                 TTTGGGGCTG 
                 TTTGGGGCTGGCCTCAC 
                 7476 
                 ATPase, H+ transporting, lysosomal (vacuolar proto 
               
               
                   
               
               
                 CACCCTGTAC 
                 CACCCTGTACAGTTGCC 
                 25450 
                 solute carrier family 29 (nucleoside transporters) 
               
               
                   
               
               
                 GGGTGGGCGT 
                 GGGTGGGCGTGCAGGGA 
                 278378 
                 karyopherin beta 2b, transportin 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 gliona_tem_only_with_tag 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 Long Tag 
                 Std Tag 
                 Localization 
               
               
                   
               
             
          
           
               
                 Hs.101382 
                 tumor necrosis factor, alpha-induced protein 2 
                 ACTCAGCCCGGCTGATG 
                 ACTCAGCCCG 
                 cytoplasmic 
                   
               
               
                   
               
               
                 Hs.102135 
                 signal sequence receptor, delta (translocon- 
                 GCTCTCTATGCTGACGT 
                 GCTCTCTATG 
                 membrane 
               
               
                   
                 associated protein delta) 
               
               
                   
               
               
                 Hs.103180 
                 DC2 protein 
                 AGAATGAAACTGCCGGG 
                 AGAATGAAAC 
                 membrane 
               
               
                   
               
               
                 Hs.105850 
                 KIAA0404 protein 
                 AAGTGGAATAAACTGCC 
                 AAGTGGAATA 
                 nuclear 
               
               
                   
               
               
                 Hs.10784 
                 chromosome 6 open reading frame 37 
                 TTTGAATCAGTGCTAGA 
                 TTTGAATCAG 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.110802 
                 von Willebrand factor 
                 TTCTGCTCTTGTGCCCT 
                 TTCTGCTCTT 
                 extracellular 
               
               
                   
               
               
                 Hs.112844 
                 maternally expressed 3 
                 TGGGAAGTGGGCTCCTT 
                 TGGGAAGTGG 
                 mitochondria 
               
               
                   
               
               
                 Hs.11607 
                 hypothetical protein FLJ32205 
                 TGGGCCCGTGTCTGGCC 
                 TGGGCCCGTG 
                 mitochondria 
               
               
                   
               
               
                 Hs.118893 
                 Melanoma associated gene 
                 ACAACGTCCAGCTGGTG 
                 ACAACGTCCA 
                 extracellular 
               
               
                   
               
               
                 Hs.119120 
                 E3 ubiquitin ligase SMURF1 
                 CCCCCTGCCCCTCTGCC 
                 CCCCCTGCCC 
                 mitochondria 
               
               
                   
               
               
                 Hs.121849 
                 microtubule-associated protein 1 light chain 3 
                 GTCTATGCCTCCCAGGA 
                 GTCTATGCCT 
                 nuclear 
               
               
                   
                 beta 
               
               
                   
               
               
                 Hs.124915 
                 hypothetical protein MGC2601 
                 GGCTGGAGCCGCTTTGG 
                 GGCTGGAGCC 
                 extracellular 
               
               
                   
               
               
                 Hs.129780 
                 tumor necrosis factor receptor superfamily, 
                 CATACCTCCTGCCCCGC 
                 CATACCTCCT 
                 membrane 
               
               
                   
                 member 4 
               
               
                   
               
               
                 Hs.135084 
                 cystatin C (amyloid angiopathy and cerebral 
                 TGCCTGCACCAGGAGAC 
                 TGCCTGCACC 
                 extracellular 
               
               
                   
                 hemorrhage) 
               
               
                   
               
               
                 Hs.136414 
                 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl- 
                 TTCCTTGTAATCAAAGA 
                 TTCCTTGTAA 
                 extracellular 
               
               
                   
                 transferase 5 
               
               
                   
               
               
                 Hs.137574 
                 coagulation factor II (thrombin) receptor-like 3 
                 TGGCGGCAGAGGCAGAG 
                 TGGCGGCAGA 
                 membrane 
               
               
                   
               
               
                 Hs.148932 
                 sema domain, transmembrane domain (TM), and 
                 CCACGTGGCTGGCTGGG 
                 CCACGTGGCT 
                 membrane 
               
               
                   
                 cytoplasmic domain, (semaphorin) 6B 
               
               
                   
               
               
                 Hs.149152 
                 rhophilin 1 
                 CTGGAGGCTGCCTCGGG 
                 CTGGAGGCTG 
                 nuclear 
               
               
                   
               
               
                 Hs.149609 
                 integrin, alpha 5 (fibronectin receptor, alpha 
                 ATGGCAACAGATCTGGA 
                 ATGGCAACAG 
                 membrane 
               
               
                   
                 polypeptide) 
               
               
                   
               
               
                 Hs.151761 
                 KIAA0100 gene product 
                 GGTCCCCTACCCTTCCC 
                 GGTCCCCTAC 
                 nuclear 
               
               
                   
               
               
                 Hs.155048 
                 Lutheran blood group (Auberger b antigen 
                 CCCGCCCCCGCCTTCCC 
                 CCCGCCCCCG 
                 membrane 
               
               
                   
                 included) 
               
               
                   
               
               
                 Hs.155223 
                 stanniocalcin 2 
                 CCCGAGGCAGAGTCGGG 
                 CCCGAGGCAG 
                 extracellular 
               
               
                   
               
               
                 Hs.155396 
                 nuclear factor (erythroid-derived 2)-like 2 
                 CTACGTGATGAAGATGG 
                 CTACGTGATG 
                 nuclear 
               
               
                   
               
               
                 Hs.155894 
                 protein tyrosine phosphatase, non-receptor type 1 
                 ATGGGTTTGCATTTTAG 
                 ATGGGTTTGC 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.155939 
                 inositol polyphosphate-5-phosphatase, 145kDa 
                 ATGGAAGTCTGCGTAAC 
                 ATGGAAGTCT 
                 nuclear 
               
               
                   
               
               
                 Hs.156351 
                 hypothetical protein FLJ23471 
                 TGGACAGCAGGGACCTG 
                 TGGACAGCAG 
                 nuclear 
               
               
                   
               
               
                 Hs.1600 
                 chaperonin containing TCP1, subunit 5 (epsilon) 
                 TCATAGAAACCTTGATT 
                 TCATAGAAAC 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.160958 
                 CDC37 cell division cycle 37 homolog 
                 CAGCGCTGCATTGACTC 
                 CAGCGCTGCA 
                 cytoplasmic 
               
               
                   
                 ( S. cerevisiae ) 
               
               
                   
               
               
                 Hs.165983 
                 zinc finger protein 335 
                 CTGGGTGCCCCAGCCTG 
                 CTGGGTGCCC 
                 nuclear 
               
               
                   
               
               
                 Hs.169401 
                 apolipoprotein E 
                 CGACCCCACGCCACCCC 
                 CGACCCCACG 
                 extracellular 
               
               
                   
               
               
                 Hs.172813 
                 Rho guanine nucleotide exchange factor (GEF) 7 
                 CGCTGGGCGTCTGGGAC 
                 CGCTGGGCGT 
                 nuclear 
               
               
                   
               
               
                 Hs.1735 
                 inhibin, beta B (activin AB beta polypeptide) 
                 ATTAGTCAGAAACTGCC 
                 ATTAGTCAGA 
                 extracellular 
               
               
                   
               
               
                 Hs.180324 
                 insulin-like growth factor binding protein 5 
                 GATAGCACAGTTGTCAG 
                 GATAGCACAG 
                 extracellular 
               
               
                   
               
               
                 Hs.180610 
                 splicing factor proline/glutamine rich 
                 CGTACTGAGCGCTTTGG 
                 CGTACTGAGC 
                 nuclear 
               
               
                   
                 (polypyrimidine tract binding protein associated) 
               
               
                   
               
               
                 Hs.18069 
                 legumain 
                 GGGGCTTCTGTAGCCCC 
                 GGGGCTTCTG 
                 extracellular 
               
               
                   
               
               
                 Hs.180842 
                 ribosomal protein L13 
                 CCCGTCCGGAACGTCTA 
                 CCCGTCCGGA 
                 nuclear 
               
               
                   
               
               
                 Hs.180920 
                 ribosomal protein S9 
                 CCAGTGGCCCGGAGCTG 
                 CCAGTGGCCC 
                 mitochondria 
               
               
                   
               
               
                 Hs.182248 
                 sequestosome 1 
                 ACTGTACTCCAGCCTAG 
                 ACTGTACTCC 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.1827 
                 nerve growth factor receptor (TNFR superfamily, 
                 AGCTCCAGACCCCCAGC 
                 AGCTCCAGAC 
                 membrane 
               
               
                   
                 member 16) 
               
               
                   
               
               
                 Hs.184245 
                 SMART/HDAC1 associated repressor protein 
                 GACTCGCAGACACCGGG 
                 GACTCGCAGA 
                 nuclear 
               
               
                   
               
               
                 Hs.184669 
                 zinc finger protein 144 (Mel-18) 
                 GGCCTCCAGCCACCCAC 
                 GGCCTCCAGC 
                 nuclear 
               
               
                   
               
               
                 Hs.19347 
                 mitochondrial ribosomal protein L45 
                 GACCAGCCTTCAGATGG 
                 GACCAGCCTT 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.194654 
                 brain-specific angiogenesis inhibitor 1 
                 GCCCCCAGGGGCAGGAC 
                 GCCCCCAGGG 
                 membrane 
               
               
                   
               
               
                 Hs.19555 
                 prostate tumor over expressed gene 1 
                 GAGGATGGTGTCCTGAG 
                 GAGGATGGTG 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.195851 
                 actin, alpha 2, smooth muscle, aorta 
                 AAGATCAAGATCATTGC 
                 AAGATCAAGA 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.201671 
                 SRY (sex determining region Y)-box 13 
                 AGCACAGGGTCGGGGGG 
                 AGCACAGGGT 
                 membrane 
               
               
                   
               
               
                 Hs.20225 
                 tuftelin interacting protein 11 
                 GCCAAGTGAACTGTGGC 
                 GCCAAGTGAA 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.202833 
                 heme oxygenase (decycling) 1 
                 CGTGGGTGGGGAGGGAG 
                 CGTGGGTGGG 
                 membrane 
               
               
                   
               
               
                 Hs.20976 
                   Homo sapiens  cDNA FLJ34888 fis, clone 
                 CTCCCCTATGGACTGGC 
                 CTCCCCTATG 
               
               
                   
                 NT2NE2017332 
               
               
                   
               
               
                 Hs.211600 
                 tumor necrosis factor, alpha-induced protein 3 
                 AGTATGAGGAAATCTCT 
                 AGTATGAGGA 
                 nuclear 
               
               
                   
               
               
                 Hs.212680 
                 tumor necrosis factor receptor superfamily, 
                 GCCCCCTTCCTCCCTTG 
                 GCCCCCTTCC 
                 membrane 
               
               
                   
                 member 18 
               
               
                   
               
               
                 Hs.21595 
                 DNA segment on chromosome X and Y (unique) 155 
                 GGGATTTCTGTGTCTGC 
                 GGGATTTCTG 
                 nuclear 
               
               
                   
                 expressed sequence 
               
               
                   
               
               
                 Hs.217493 
                 annexin A2 
                 CTTCCAGCTAACAGGTC 
                 CTTCCAGCTA 
                 nuclear 
               
               
                   
               
               
                 Hs.2250 
                 leukemia inhibitory factor (cholinergic 
                 GCCTTGGGTGACAAATT 
                 GCCTTGGGTG 
                 extracellular 
               
               
                   
                 differentiation factor) 
               
               
                   
               
               
                 Hs.23131 
                 kinesin family member C3 
                 GCCTCCCGCCACGGGGC 
                 GCCTCCCGCG 
                 nuclear 
               
               
                   
               
               
                 Hs.2340 
                 junction plakoglobin 
                 GTGTGGGGGGCTGGGGG 
                 GTGTGGGGGG 
                 nuclear 
               
               
                   
               
               
                 Hs.234726 
                 serine (or cysteine) proteinase inhibitor, clade 
                 GACTCTTCAGTCTGGAG 
                 GACTCTTCAG 
                 extracellular 
               
               
                   
                 A (alpha-1 antiproteinase, antitrypsin), 
               
               
                   
                 member 3 
               
               
                   
               
               
                 Hs.236516 
                 C-type (calcium dependent, carbohydrate- 
                 GCCACACCCACCGCCCC 
                 GCCACACCCA 
                 membrane 
               
               
                   
                 recognition domain) lectin, superfamily member 9 
               
               
                   
               
               
                 Hs.240443 
                 multiple endocrine neoplasia I 
                 CCAGGGCAACAGAATGA 
                 CCAGGGCAAC 
                 nuclear 
               
               
                   
               
               
                 Hs.25450 
                 solute carrier family 29 (nucleoside 
                 CACCCTGTACAGTTGCC 
                 CACCCTGTAC 
                 membrane 
               
               
                   
                 transporters), member 1 
               
               
                   
               
               
                 Hs.25590 
                 stanniocalcin 1 
                 GACGAATATGAATGTCA 
                 GACGAATATG 
                 extracellular 
               
               
                   
               
               
                 Hs.25590 
                 stanniocalcin 1 
                 CAAACTGGTCTAGGTCA 
                 CAAACTGGTC 
                 extracellular 
               
               
                   
               
               
                 Hs.25590 
                 stanniocalcin 1 
                 GTAATGACAGATGCAAG 
                 GTAATGACAG 
                 extracellular 
               
               
                   
               
               
                 Hs.268571 
                 apolipoprotein C-I 
                 TGGCCCCAGGTGCCACC 
                 TGGCCCCAGG 
                 extracellular 
               
               
                   
               
               
                 Hs.272927 
                 Sec23 homolog A ( S. cerevisiae ) 
                 AACACAATCATATGATG 
                 AACACAATCA 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.274184 
                 transcription factor binding to IGHM enhancer 3 
                 GAGGGTATACTGAGGGG 
                 GAGGGTATAC 
                 nuclear 
               
               
                   
               
               
                 Hs.274453 
                 likely ortholog of mouse embryonic epithelial 
                 GGAGCCAGCTGACCTGC 
                 GGAGCCAGCT 
                 membrane 
               
               
                   
                 gene 1 
               
               
                   
               
               
                 Hs.278361 
                 likely ortholog of mouse fibronectin type III 
                 GAGCCTCAGGTGCTCCC 
                 GAGCCTCAGG 
                 membrane 
               
               
                   
                 repeat containing protein 
               
               
                   
               
               
                 Hs.278573 
                 CD59 antigen p18-20 (antigen identified by 
                 TACTTCACATACAGTGC 
                 TACTTCACAT 
                 extracellular 
               
               
                   
                 monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 
               
               
                   
                 and G344) 
               
               
                   
               
               
                 Hs.286035 
                 myosin XVB, pseudogene 
                 CGGTGGGACCACCCTGC 
                 CGGTGGGACC 
                 nuclear 
               
               
                   
               
               
                 Hs.286035 
                 myosin XVB, pseudogene 
                 GGAGAAACAGCTGCTGA 
                 GGAGAAACAG 
                 nuclear 
               
               
                   
               
               
                 Hs.288203 
                   Homo sapiens , clone IMAGE:4845226, mRNA 
                 GCTCAGGTCTGCCGGGG 
                 GCTCAGGTCT 
               
               
                   
               
               
                 Hs.288991 
                 TNFAIP3 interacting protein 2 
                 TCTGCACTGAGAAACTG 
                 TCTGCACTGA 
                 nuclear 
               
               
                   
               
               
                 Hs.296406 
                 KIAA0685 gene product 
                 TCCACGCCCTTCCTGGC 
                 TCCACGCCCT 
                 nuclear 
               
               
                   
               
               
                 Hs.29716 
                 hypothetical protein FLJ10980 
                 TTGCAATAGCAAAACCC 
                 TTGCAATAGC 
                 nuclear 
               
               
                   
               
               
                 Hs.297753 
                 vimentin 
                 TCCAAATCGATGTGGAT 
                 TCCAAATCGA 
                 mitochondria 
               
               
                   
               
               
                 Hs.29797 
                 ribosomal protein L10 
                 AGGGCTTCCAATGTGCT 
                 AGGGCTTCCA 
                 mitochondrial 
               
               
                   
               
               
                 Hs.299257 
                 STs, Weakly similar to hypothetical protein 
                 AACCTGGGAGGTGGAGG 
                 AACCTGGGAG 
               
               
                   
                 FLJ20489 [ Homo sapiens ] [ H.sapiens ] 
               
               
                   
               
               
                 Hs.301242 
                 likely ortholog of mouse myocytic induction/ 
                 GGCCAACATTTGGTCCA 
                 GGCCAACATT 
                 cytoplasmic 
               
               
                   
                 differentiation originator 
               
               
                   
               
               
                 Hs.301685 
                 KIAA0620 protein 
                 GGGGCTGGAGGGGGGCA 
                 GGGGCTGGAG 
                 membrane 
               
               
                   
               
               
                 Hs.302741 
                 Homo sapiens mRNA full length insert cDNA clone 
                 GGATGCGCAGGGGAGGC 
                 GGATGCGCAG 
               
               
                   
                 EUROIMAGE 50374 
               
               
                   
               
               
                 Hs.318751 
                 ESTs, Weakly similar to T21371 hypothetical 
                 GAAGACACTTGGTTTGA 
                 GAAGACACTT 
               
               
                   
                 protein F25H8.3  Caenorhabditis elegans   
               
               
                   
                 [ C.elegans ] 
               
               
                   
               
               
                 Hs.321231 
                 UDP-Gal:betaGlcNAc beta 1,4-galactosyl- 
                 GAGAGAAGAGTGATCTG 
                 GAGAGAAGAG 
                 extracellular 
               
               
                   
                 transferase, polypeptide 3 
               
               
                   
               
               
                 Hs.326445 
                 v-akt murine thymoma viral oncogene homolog 2 
                 GCAGGGTGGGGAGGGGT 
                 GCAGGGTGGG 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.334604 
                 KIAA1870 protein 
                 TCAGTGTATTAAAACCC 
                 TCAGTGTATT 
                 extracellular 
               
               
                   
               
               
                 Hs.339283 
                 endoplasmic reticulum associated protein 140 kDa 
                 ATACTATAATTGTGAGA 
                 ATACTATAAT 
                 nuclear 
               
               
                   
               
               
                 Hs.34516 
                 ceramide kinase 
                 GCTGGTTCCTGAGTGGC 
                 GCTGGTTCCT 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.348000 
                 ESTs, Weakly similar to hypothetical protein 
                 AGCCACTGCGCCCGGCC 
                 AGCCACTGCG 
               
               
                   
                 FLJ20489 [ Homo sapiens] [   H.sapiens ] 
               
               
                   
               
               
                 Hs.350065 
                 hypothetical protein FLJ30634 
                 AGACTAGGGGCCGGAGC 
                 AGACTAGGGG 
                 nuclear 
               
               
                   
               
               
                 Hs.352535 
                 KIAA0943 protein 
                 GGGACAGCTGTCTGTGG 
                 GGGACAGCTG 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.352949 
                 ESTs, Weakly similar to hypothetical protein 
                 AACCCAGGAGGCGGAGC 
                 AACCCAGGAG 
               
               
                   
                 FLJ20489 [ Homo sapiens ] [ H.sapiens ] 
               
               
                   
               
               
                 Hs.353002 
                 ESTs 
                 CAGCCTGAGGCTCTTGG 
                 CAGCCTGAGG 
               
               
                   
               
               
                 Hs.353193 
                 LOC124402 
                 CCTCCCCTGCACCTGGG 
                 CCTCCCCTGG 
                 nuclear 
               
               
                   
               
               
                 Hs.363027 
                   Homo sapiens  cDNA FLJ39848 fis, clone 
                 GCTTCAGTGGGGGAGAG 
                 GCTTCAGTGG 
               
               
                   
                 SPLEN2014669 
               
               
                   
               
               
                 Hs.367653 
                 hypothetical protein FLJ22329 
                 TGTTTGGGGGCTTTTAG 
                 TGTTTGGGGG 
                 extracellular 
               
               
                   
               
               
                 Hs.373548 
                   Homo sapiens  cDNA: FLJ22720 fis, clone HS114320 
                 TTTTAAATTAGGTTTTG 
                 TTTTAAATTA 
               
               
                   
               
               
                 Hs.374415 
                 ESTs 
                 ATCTCAAAGATACACAG 
                 ATCTCAAAGA 
               
               
                   
               
               
                 Hs.39619 
                 hypothetical protein LOC57333 
                 TTTGTGGGCAGTCAGG 
                 CTTTGTGGGCA 
                 extracellular 
               
               
                   
               
               
                 Hs39871 
                 myosin ID 
                 ATTGTAGACAATGAGGG 
                 ATTGTAGACA 
                 nuclear 
               
               
                   
               
               
                 Hs.400429 
                 ESTs 
                 GCAAAACCCTGCTCTCC 
                 GCAAAACCCT 
               
               
                   
               
               
                 Hs.401975 
                 ESTs, Weakly similar to TI7346 hypothetical 
                 GTCTCAGTGCTGAGGCG 
                 GTCTCAGTGC 
               
               
                   
                 protein DKPZp586O1624.1 - human (fragment) 
               
               
                   
                 [ H.sapiens ] 
               
               
                   
               
               
                 Hs.405289 
                 ESTs, Weakly similar to hypothetical protein 
                 AGCCACTGTGCCCGGCC 
                 AGCCACTGTG 
               
               
                   
                 FLJ20378 [ Homo sapiens ] [ H.sapiens ] 
               
               
                   
               
               
                 Hs.406068 
                 ubiquitin-conjugating enzyme E2M (UBC12 homolog, 
                 TGATTAAGGTCGGCGCT 
                 TGATTAAGGT 
                 nuclear 
               
               
                   
                 yeast) 
               
               
                   
               
               
                 Hs.406507 
                 sprouty homolog 4 ( Drosophila ) 
                 TTACAAACAGAAAAGCT 
                 TTACAAACAG 
                 extracellular 
               
               
                   
               
               
                 Hs.41716 
                 endothelial cell-specific molecule 1 
                 TTTATTATTGTTCAATA 
                 TTTATTATTG 
                 extracellular 
               
               
                   
               
               
                 Hs.45006 
                 hypothetical protein DKFZp547N157 
                 CGGGCCTCAGGTGGCAG 
                 CGGCCCTCAG 
                 nuclear 
               
               
                   
               
               
                 Hs.4980 
                 LIM domain binding 2 
                 TAAAGGCACAGTGGCTC 
                 TAAAGGCACA 
                 nuclear 
               
               
                   
               
               
                 Hs.5307 
                 synaptopodin 
                 ATATTAGGAAGTCGGGG 
                 ATATTAGGAA 
                 nuclear 
               
               
                   
               
               
                 Hs.56205 
                 insulin induced gene 1 
                 TGATTAAAACAAGTTGC 
                 TGATTAAAAC 
                 membrane 
               
               
                   
               
               
                 Hs.57958 
                 EGF-TM7-latrophilin-related protein 
                 TTGTGCACGCATCAGTG 
                 TTGTGCAGGC 
                 membrane 
               
               
                   
               
               
                 Hs.61490 
                 schwannomin interacting protein 1 
                 CCTGCCTCGTAGTGAAG 
                 CCTGCCTCGT 
                 nuclear 
               
               
                   
               
               
                 Hs.61638 
                 myosin X 
                 CAAAACTGTTTGTTGGCC 
                 CAAAACTGTT 
                 nuclear 
               
               
                   
               
               
                 Hs.62192 
                 coagulation factor III (thromboplastin, tissue 
                 TAGGAAAGTAAAATGGA 
                 TAGGAAAGTA 
                 membrane 
               
               
                   
                 factor) 
               
               
                   
               
               
                 Hs.65238 
                 ring finger protein 40 
                 CTCCATCGGCTGTGAGG 
                 CTCCATCGGC 
                 nuclear 
               
               
                   
               
               
                 Hs.6657 
                 Hermansky-Pudlak syndrome 4 
                 CAAGCATCCCCGTTCCA 
                 CAAGCATCCC 
                 nuclear 
               
               
                   
               
               
                 Hs.6631 
                 golgi complex associated protein 1, 60kDa 
                 GAGTTAGGCACTTCCTG 
                 GAGTTAGGCA 
                 nuclear 
               
               
                   
               
               
                 Hs.69954 
                 laminin, gamma 3 
                 CATAAACGGGCACACCC 
                 CATAAACGGG 
                 extracellular 
               
               
                   
               
               
                 Hs.7187 
                 hypothetical protein FLJ10707 
                 TTGCCTGGGATGCTGGT 
                 TTGCCTGGGA 
                 nuclear 
               
               
                   
               
               
                 Hs.73798 
                 macrophage migration inhibitory factor 
                 AACGCGGCCAATGTGGG 
                 AACGCGGCCA 
                 cytoplasmic 
               
               
                   
                 (glycosylation-inhibiting factor) 
               
               
                   
               
               
                 Hs.73818 
                 ubiquinol-cytochrome c reductase hinge protein 
                 GGTTTGGCTTAGGCTGG 
                 GGTTTGGCTT 
                 nuclear 
               
               
                   
               
               
                 Hs.74471 
                 gap junction protein, alpha 1, 43kDa 
                 GATTTTTGTGGTGTGGG 
                 GATTTTTGTG 
                 membrane 
               
               
                   
                 (connexin 43) 
               
               
                   
               
               
                 Hs.74566 
                 dihydropyrimidinase-like 3 
                 GGCTGCCCTGGGCAGCC 
                 GGCTGCCCTG 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.74602 
                 aquaporin 1 (channel-forming integral protein, 
                 ATGGCAACAGAAACCAA 
                 ATGGCAACAG 
                 membrane 
               
               
                   
                 28kDa) 
               
               
                   
               
               
                 Hs.75093 
                 procoilagen-lysine, 2-oxoglutarate 5-dioxygenase 
                 AGAGCAAACCGTAGTCC 
                 AGAGCAAACC 
                 extracellular 
               
               
                   
                 (lysine hydroxylase, Ehiers-Danlos syndrome type 
               
               
                   
                 VI) 
               
               
                   
               
               
                 Hs.75445 
                 SPARC-like 1 (mastg, hevin) 
                 TGCACTTCAAGAAAATG 
                 TGCACTTCAA 
                 extracellular 
               
               
                   
               
               
                 Hs.75736 
                 apolipoprotein D 
                 CCCTACCCTGTTACCTT 
                 CCCTACCCTG 
                 extracellular 
               
               
                   
               
               
                 Hs.76353 
                 serine (or cysteine) proteinase inhibitor, clade 
                 GGAAAAATGTTGGMTG 
                 GGAAAAATGT 
                 extracellular 
               
               
                   
                 A (alpha-1 antiproteinase, antitrypsin), member 5 
               
               
                   
               
               
                 Hs.7718 
                 hypothetical protein FLJ22678 
                 GTTTTTGCTTCAGCGGC 
                 GTTTTTGCTT 
                 extracellular 
               
               
                   
               
               
                 Hs.77313 
                 cyclin-dependent kinase (CDC2-like) 10 
                 GAGGACCCAACAGGAGG 
                 GAGGACCCAA 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.77326 
                 insulin-like growth factor binding protein 3 
                 ACTGAGGAAAGGAGCTC 
                 ACTGAGGAAA 
                 extracellular 
               
               
                   
               
               
                 Hs.77573 
                 uridine phosphorylase 
                 TGCAGCGCCTGCGGCCT 
                 TGCAGCGCCT 
                 nuclear 
               
               
                   
               
               
                 Hs.77864 
                 K1AA0638 protein 
                 CTGGGGGGAAGGGACTG 
                 CTGGGGGGAA 
                 nuclear 
               
               
                   
               
               
                 Hs.77886 
                 lamin A/C 
                 GTGCCTGAGAGGCAGGC 
                 GTGCCTGAGA 
                 nuclear 
               
               
                   
               
               
                 Hs.77886 
                 lamin A/C 
                 TCACAGGGTCCCCGGGG 
                 TCACAGGGTC 
                 nuclear 
               
               
                   
               
               
                 Hs.77866 
                 lamin A/C 
                 GGAGGGGGCTTGAAGCC 
                 GGAGGGGGCT 
                 nuclear 
               
               
                   
               
               
                 Hs.78056 
                 cathepsin L 
                 GGAGGAATTCATCTTCA 
                 GGAGGAATTC 
                 extracellular 
               
               
                   
               
               
                 Hs.78531 
                 similar to RIKEN cDNA 5730528L13 gene 
                 GAAAGTGGCTGTCCTGG 
                 GAAAGTGGCT 
                 nuclear 
               
               
                   
               
               
                 Hs.78575 
                 prosaposin (variant Gaucher disease and variant 
                 TCCCTGGCTGTTGAGGC 
                 TCCCTGGCTG 
                 extracellular 
               
               
                   
                 metachromatic leukodystrophy) 
               
               
                   
               
               
                 Hs.82575 
                 small nuclear ribonucleoprotein polypeptide B″ 
                 AAGATGAGGGGGCAGGC 
                 AAGATGAGGG 
                 nuclear 
               
               
                   
               
               
                 Hs.82749 
                 transmembrane 4 superfamily member 2 
                 CCAACAAGAATGCATTG 
                 CCAACAAGAA 
                 membrane 
               
               
                   
               
               
                 Hs.83126 
                 TAF11 RNA polymerase II, TATA box binding protein 
                 AAGGATGCGGTGATGGC 
                 AAGGATGCGG 
                 nuclear 
               
               
                   
                 (TBP)-associated factor, 28kDa 
               
               
                   
               
               
                 Hs.83169 
                 matrix metalloproteinase 1 (interstitial 
                 TGCAGTCACTGGTGTCA 
                 TGCAGTCACT 
                 extracllular 
               
               
                   
                 collagenase) 
               
               
                   
               
               
                 Hs.83384 
                 S100 calcium binding protein, beta (neural) 
                 GCCGTGTAGACCCTAAC 
                 GCCGTGTAGA 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.83484 
                 SRY (sex determining region Y)-box 4 
                 CAGGCTTTTTGGCTTCC 
                 CAGGCTTTTT 
                 nuclear 
               
               
                   
               
               
                 Hs.83464 
                 SRY (sex determining region Y)-box 4 
                 TCCCTGGGCAGCTTCAG 
                 TCCCTGGGCA 
                 nuclear 
               
               
                   
               
               
                 Hs.63727 
                 cleavage and polyadenylation specific factor 1, 
                 GAGCGCAGCGAGCTAGC 
                 GAGCGCAGCG 
                 nuclear 
               
               
                   
                 160kDa 
               
               
                   
               
               
                 Hs.84063 
                   Homo sapiens  cDNA: FLJ23507 fis, clone LNG03128 
                 CAGGTGGTTCTGCCATC 
                 CAGGTGGTTC 
               
               
                   
               
               
                 Hs.84753 
                 hypothetical protein FLJ12442 
                 GCCCACATCCGCTGAGG 
                 GCCCACATCC 
                 cytoplasmic 
               
               
                   
               
               
                 Hs.89695 
                 insulin receptor 
                 AAGGTTCTTCTCAAGGG 
                 AAGGTTCTTC 
                 membrane 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 11 
               
             
             
               
                   
               
               
                 Glioma Repressed in Transporters 
               
             
          
           
               
                 Short Tag 
                 Long Tag 
                 UG ID 
                 UG Description 
               
               
                   
               
             
          
           
               
                 GGCTAATTAT** 
                 GGCTAATTATCATCAAT 
                 34114 
                 ATPase, Na+/K+ transporting alpha 2(+) polypeptide 
                   
               
               
                   
               
               
                 CAAAAATAAA 
                 CAAAAATAAAAGCCGA 
                 30246 
                 solute carrier family 19 (thiamine transporter), m 
               
               
                   
                   
                   
                 *Transport* 
               
               
                   
               
               
                 **Also present in Glioma repressed list 
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 12 
               
             
             
               
                   
               
               
                 Nuclear Brain and Colon Proteins 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.149098 
                 smoothelin 
                 602127 
                 NP_599031 
               
               
                 Hs.197298 
                 NS1-binding protein 
                   
                 AAG43485 
               
               
                 Hs.337986 
                 hypothetical protein MGC4677 
                   
                 NP_443103 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 13 
               
             
             
               
                   
               
               
                 Cytoplasmic Brain/Colon Proteins 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.327412 
                 TEM 15, COL3A1,  Homo   
                   
                   
               
               
                   
                   sapiens  clone FLC1492 
               
               
                   
                 PRO3121 mRNA, complete cds 
               
               
                 Hs.75721 
                 profilin 1 
                 176610 
                 NP_005013 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 14 
               
             
             
               
                   
               
               
                 Extracellular Colon/Brain Proteins 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.1103 
                 transforming growth factor, beta 
                 190180 
                 NP_000651 
               
               
                   
                 1 (Camuratl-Engelmann 
               
               
                   
                 disease) 
               
               
                 Hs.111779 
                 secreted protein, acidic, 
                 182120 
                 NP_003109 
               
               
                   
                 cysteine-rich (osteonectin) 
               
               
                 Hs.119129 
                 collagen, type IV, alpha 1 
                 120130 
                 NP_001836 
               
               
                 Hs.119571 
                 collagen, type III, alpha 1 
                 120180 
                 NP_000081 
               
               
                   
                 (Ehlers-Danlos syndrome type 
               
               
                   
                 IV, autosomal dominant 
               
               
                 Hs.151738 
                 matrix metalloproteinase 9 
                 120361 
                 NP_004985 
               
               
                   
                 (gelatinase B, 92 kDa 
               
               
                   
                 gelatinase, 92 kDa type IV 
               
               
                   
                 collagenase) 
               
               
                 Hs.159263 
                 collagen, type VI, alpha 2 
                 120240 
                 NP_001840 
               
               
                 Hs.172928 
                 collagen, type I, alpha 1 
                 120150 
                 NP_000079 
               
               
                 Hs.179573 
                 TEM 40, COL1A2 alt polyA; 
                 120160 
                 NP_000080 
               
               
                   
                 involved in tissue remodeling 
               
               
                 Hs.75617 
                 collagen, type IV, alpha 2 
                 120090 
                 NP_001837 
               
               
                 Hs.78672 
                 laminin, alpha 4 
                 600133 
                 NP_002281 
               
               
                 Hs.821 
                 biglycan 
                 301870 
                 NP_001702 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 15 
               
             
             
               
                   
               
               
                 Membrane Brain/Colon Proteins 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.125359 
                 TEM 13, Thy-1 cell surface 
                 188230 
                 NP_006279 
               
               
                   
                 antigen 
               
               
                 Hs.185973 
                 degenerative spermatocyte 
                   
                 NP_003667 
               
               
                   
                 homolog, lipid desaturase 
               
               
                   
                 ( Drosophila ) 
               
               
                 Hs.195727 
                 TEM 1, endosialin 
                 606064 
                 NP_065137 
               
               
                 Hs.23016 
                 G protein-coupled receptor 
               
               
                 Hs.2399 
                 matrix metalloproteinase 14 
                 600754 
                 NP_004986 
               
               
                   
                 (membrane-inserted) 
               
               
                 Hs.285814 
                 sprouty homolog 4 ( Drosophilia ) 
                   
                 AAK00653 
               
               
                 Hs.82002 
                 endothelin receptor type B 
                 131244 
                 NP_000106 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 16 
               
             
             
               
                   
               
               
                 Brain and Colon Proteins 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.1103 
                 transforming growth factor, beta 1 (Camurati- 
                 190180 
                 NP_000651 
               
               
                   
                 Engelmann disease) 
               
               
                 Hs.111779 
                 secreted protein, acidic, cysteine-rich 
                 182120 
                 NP_003109 
               
               
                   
                 (osteonectin) 
               
               
                 Hs.119129 
                 collagen, type IV, alpha 1 
                 120130 
                 NP_001836 
               
               
                 Hs.119571 
                 collagen, type III, alpha 1 (Ehlers-Danios 
                 120180 
                 NP_000081 
               
               
                   
                 syndrome type IV, autosomal dominant) 
               
               
                 Hs.125359 
                 TEM 13, Thy-1 cell surface antigfen 
                 188230 
                 NP_006279 
               
               
                 Hs.149098 
                 smoothelin 
                 602127 
                 NP_599031 
               
               
                 Hs.151738 
                 matrix metalloproteinase 9 (gelatinase B, 
                 120361 
                 NP_004985 
               
               
                   
                 92 kDa gelatinase, 92 kDa type IV collagenase) 
               
               
                 Hs.159263 
                 collagen, type VI, alpha 2 
                 120240 
                 NP_001840 
               
               
                 Hs.172928 
                 collagen, type 1, alpha 1 
                 120150 
                 NP_000079 
               
               
                 Hs.179573 
                 TEM 40, COL1A2 alt polyA; involved in tissue 
                 120160 
                 NP_000080 
               
               
                   
                 remodeling 
               
               
                 Hs.185973 
                 degenerative spermatocyte homolog, lipid 
                   
                 NP_003667 
               
               
                   
                 desaturase ( Drosophila ) 
               
               
                 Hs.195727 
                 TEM 1, endosialin 
                 606064 
                 NP_065137 
               
               
                 Hs.197298 
                 NS1-binding protein 
                   
                 AAG43485 
               
               
                 Hs.23016 
                 G protein-coupled receptor 
               
               
                 Hs.2399 
                 matrix metalloproteinase 14 (membrane- 
                 600754 
                 NP_004986 
               
               
                   
                 inserted) 
               
               
                 Hs.285814 
                 sprouty homolog 4 ( Drosophila ) 
                   
                 AAK00653 
               
               
                 Hs.327412 
                 TEM15, COL311,  Homo sapiens  clone 
               
               
                   
                 FLC1492 PRO3121 mRNA, complete cds 
               
               
                 Hs.337986 
                 hypotehtical protein MGC4677 
                   
                 NP_443103 
               
               
                 Hs.351928 
                   Homo sapiens  mRNA full length insert cDNA 
               
               
                   
                 clone EUROIMAGE 1977059 
               
               
                 Hs.356096 
                 ESTs, Highly similar to hypothetical protein 
               
               
                   
                 FLJ10350 [ Homo sapiens ] [ H. sapiens ] 
               
               
                 Hs.75617 
                 collagen, type IV, alpha 2 
                 120090 
                 NP_001837 
               
               
                 Hs.75721 
                 profilin 1 
                 176610 
                 NP_005013 
               
               
                 Hs.78672 
                 laminin, alpha 4 
                 600133 
                 NP_002281 
               
               
                 Hs.82002 
                 endothelin receptor type B 
                 131244 
                 NP_000106 
               
               
                 Hs.821 
                 biglycan 
                 301870 
                 NP_001702 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 17 
               
             
             
               
                   
               
               
                 Additional Tumor Endothelial Markers in Brain 
               
             
          
           
               
                 Unigene ID 
                 Function 
                   
               
               
                   
               
               
                 Hs.326445 
                 v-akt murine thymoma vial oncogene homolog 2 
                 Protein Kinase 
               
               
                 Hs.77313 
                 cyclin-dependent kinase (cdc2-like) 10 
                 Protein Kinase 
               
               
                 Hs.301242 
                 ortholog mouse myocytic 
                 Non-Protein Kinase 
               
               
                   
                 induction/differntiation originator 
               
               
                 Hs.194654 
                 brain-specific angiogenesis inhibitor 1 
                 Membrane GPCR 
               
               
                 Hs.57958 
                 EGF-RM7 latrophilin-related protein 
                 Membrane GPCR 
               
               
                 Hs.148932 
                 sema domain 
                 Receptors with Short Cytoplasmic Tail 
               
               
                 Hs.149609 
                 integrin, alpha 5 
                 Receptors with Short Cytoplasmic Tail 
               
               
                 Hs.27836 
                 likely ortholog of mouse fibronectin type III 
                 Receptors with Short Cytoplasmic Tail 
               
               
                 Hs.155048 
                 Lutheran blood group (Auberger b antigen 
                 Receptors with Short Cytoplasmic Tail 
               
               
                   
                 included) 
               
               
                 Hs.102135 
                 SSR4, TRAPD 
                 Receptors with Short Cytoplasmic Tail 
               
               
                 Hs.1827 
                 nerve growth factor receptor (TNFR 
                 Membrane Receptor 
               
               
                   
                 superfamily, member 16) 
               
               
                 Hs.41716 
                 insulin-like growth factor binding protein 
                 Extracellular Growth Factors &amp; Cytokine 
               
               
                 Hs.2250 
                 leukemia inhibitor factor 
                 Extracellular Growth Factors &amp; Cytokine 
               
               
                 Hs.155894 
                 protein typrosine phosphatase, nonreceptor 
                 Cell-Selective Phosphatase 
               
               
                   
                 type I 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 18 
               
             
             
               
                   
               
               
                 Cytoplasmic GEMs 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.111611 
                 ribosomal protein L27 
                 607526 
                 NP_000979 
               
               
                 Hs.160958 
                 CDC37 cell division cycle 37 
                 605065 
                 NP_008996 
               
               
                   
                 homolog ( S. cerevisiae ) 
               
               
                 Hs.327412 
                 TEM15, COLI3A1,  Homo   
               
               
                   
                   sapiens  clone FLC1492 
               
               
                   
                 PRO3121 mRNA, complete cds 
               
               
                 Hs.34516 
                 ceramide kinase 
                   
                 NP_073603 
               
               
                 Hs.352535 
                 KIAA0943 protein 
                   
                 BAA76787 
               
               
                 Hs.61661 
                 F-box only protein 32 
                 606604 
                 NP_478136 
               
               
                 Hs.73798 
                 macrophage migration 
                 153620 
                 NP_002406 
               
               
                   
                 inhibitory factor (glycosylation- 
               
               
                   
                 inhibiting factor) 
               
               
                 Hs.75721 
                 profilin 1 
                 176610 
                 NP_005013 
               
               
                 Hs.83384 
                 S100 calcium binding protein, 
                 176990 
                 NP_006263 
               
               
                   
                 beta (neural) 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 19 
               
             
             
               
                   
               
               
                 Nuclear GEMs 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.105850 
                 KIAA0404 protein 
                   
                 BAA23700 
               
               
                 Hs.110443 
                 hypothetical protein FLJ22215 
                   
                 NP_073745 
               
               
                 Hs.121849 
                 microtubule-associated protein 1 light 
                   
                 NP_073729 
               
               
                   
                 chain 3 beta 
               
               
                 Hs.129673 
                 eukaryotic translation initiation factor 
                 602641 
                 NP_001407 
               
               
                   
                 4A, isoform 1 
               
               
                 Hs.149098 
                 smoothelin 
                 602127 
                 NP_599031 
               
               
                 Hs.155396 
                 nuclear factor (erythroid-derived 2)-like 2 
                 600492 
                 NP_006155 
               
               
                 Hs.172813 
                 Rho guanine nucleotide exchange factor 
                 605477 
                 NP_663788 
               
               
                   
                 (GEF) 7 
               
               
                 Hs.197298 
                 NS1-binding protein 
                   
                 AAG43485 
               
               
                 Hs.211600 
                 tumor necrosis factor, alpha-induced protein 3 
                 191163 
                 NP_006281 
               
               
                 Hs.217493 
                 annexin A2 
                 151740 
                 — 
               
               
                 Hs.2340 
                 junction plakoglobin 
                 173325 
                 NP_002221 
               
               
                 Hs.274184 
                 transcription factor binding to IGHM 
                 314310 
                 NP_006512 
               
               
                   
                 enhancer 3 
               
               
                 Hs.286035 
                 myosin XVB, pseudogene 
               
               
                 Hs.332173 
                 transducin-like enhancer of split 2 
                 601041 
                 NP_003251 
               
               
                   
                 (E(sp1) homolog,  Drosophila ) 
               
               
                 Hs.337986 
                 hypothetical protein MGC4677 
                   
                 NP_443103 
               
               
                 Hs.339283 
                 endoplasmic reticulum associated 
               
               
                   
                 protein 140 kDa 
               
               
                 Hs.350065 
                 hypothetical protein FLJ30634 
                   
                 NP_694559 
               
               
                 Hs.65238 
                 ring finger protein 40 
                   
                 NP_055586 
               
               
                 Hs.6657 
                 Hermansky-Pudlak syndrome 4 
                 606682 
                 BAB33337 
               
               
                 Hs.75061 
                 MARCKS-like protein 
                 602940 
                 NP_075385 
               
               
                 Hs.77573 
                 uridine phosphorylase 
                 191730 
                 NP_003355 
               
               
                 Hs.77886 
                 lamin A/C 
                 150330 
                 NP_005563 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 20 
               
             
             
               
                   
               
               
                 Membrane GEMs 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.107125 
                 plasmalemina vesicle 
                   
                 NP_112600 
               
               
                   
                 associated protein 
               
               
                 Hs.125359 
                 TEM13, Thy-1 cell 
                 188230 
                 NP_006279 
               
               
                   
                 surface antigen 
               
               
                 Hs.137574 
                 coagulation factor II 
                 602779 
                 NP_003941 
               
               
                   
                 (thrombin) receptor-like 3 
               
               
                 Hs.143897 
                 dysterlin, limb girdle 
                 603009 
                 NP_003485 
               
               
                   
                 muscular dystrophy 2B 
               
               
                   
                 (autosomal recessive) 
               
               
                 Hs.148932 
                 sema domain, 
                   
                 NP_115484 
               
               
                   
                 transmembrane domain 
               
               
                   
                 (TM), and cytoplasmic 
               
               
                   
                 domain, (semaphorin) 6B 
               
               
                 Hs.149609 
                 integrin, alpha 5 
                 135620 
                 NP_002196 
               
               
                   
                 (fibronectin receptor, 
               
               
                   
                 alpha polypeptide) 
               
               
                 Hs.166254 
                 likely ortholog of rat 
                   
                 NP_112200 
               
               
                   
                 vacuole membrane 
               
               
                   
                 protein 1 
               
               
                 Hs.1827 
                 nerve growth factor 
                 162010 
                 NP_002498 
               
               
                   
                 receptor (TNFR 
               
               
                   
                 superfamily, member 16) 
               
               
                 Hs.185973 
                 degenerative 
                   
                 NP_003667 
               
               
                   
                 spermatocyte homolog, 
               
               
                   
                 lipid desaturase 
               
               
                   
                 ( Drosophila ) 
               
               
                 Hs.195727 
                 TEM1, endosialin 
                 606064 
                 NP_065137 
               
               
                 Hs.202833 
                 heme oxygenase 
                 141250 
                 NP_002124 
               
               
                   
                 (decycling) 1 
               
               
                 Hs.23016 
                 G protein-coupled 
               
               
                   
                 receptor 
               
               
                 Hs.236516 
                 C-type (calcium 
                   
                 NP_055173 
               
               
                   
                 dependent, carbohydrate- 
               
               
                   
                 recognition domain) 
               
               
                   
                 lectin, superfamily 
               
               
                   
                 member 9 
               
               
                 Hs.2399 
                 matrix metalloproteinase 
                 600754 
                 NP_004986 
               
               
                   
                 14 (membrane-inserted) 
               
               
                 Hs.25450 
                 solute carrier family 29 
                 602193 
                 NP_004946 
               
               
                   
                 (nucleoside transporters), 
               
               
                   
                 member 1 
               
               
                 Hs.274453 
                 likely ortholog of mouse 
                   
                 NP_060081 
               
               
                   
                 embryonic epithelial gene 1 
               
               
                 Hs.277477 
                 major histocompatibility 
                 142840 
                 NP_002108 
               
               
                   
                 complex, class I, C 
               
               
                 Hs.27836 
                 likely ortholog of mouse 
                   
                 NP_073734 
               
               
                   
                 fibronectin type III repeat 
               
               
                   
                 containing protein 1 
               
               
                 Hs.285814 
                 sprouty homolog 4 
                   
                 AAK00653 
               
               
                   
                 ( Drosophila ) 
               
               
                 Hs.301685 
                 KIAA0620 protein 
                   
                 BAA31595 
               
               
                 Hs.62192 
                 coagulation factor III 
                 134390 
                 NP_001984 
               
               
                   
                 (thromboplastin, tissue 
               
               
                   
                 factor) 
               
               
                 Hs.74602 
                 aquaporin 1 (channel- 
                 110450 
                 AAH22486 
               
               
                   
                 forming integral protein, 
               
               
                   
                 28 kDa) 
               
               
                 Hs.77961 
                 major histocompatibility 
                 142830 
                 NP_005505 
               
               
                   
                 complex, class I, B 
               
               
                 Hs.79356 
                 Lysosomal-associated 
                 601476 
                 NP_006753 
               
               
                   
                 multispanning membrane 
               
               
                   
                 protein-5 
               
               
                 Hs.82002 
                 endothelin receptor type B 
                 131244 
                 NP_000106 
               
               
                 Hs.89695 
                 insulin receptor 
                 147670 
                 NP_000199 
               
               
                 Hs.97199 
                 complement component 
                 120577 
                 NP_036204 
               
               
                   
                 1, q subcomponent, 
               
               
                   
                 receptor 1 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 21 
               
             
             
               
                   
               
               
                 Extracellular GEMS 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.1103 
                 transforming growth factor, 
                 190180 
                 NP_000651 
               
               
                   
                 beta 1 (Camurati-Engelmann 
               
               
                   
                 disease) 
               
               
                 Hs.110802 
                 von Willebrand factor 
                 193400 
                 NP_000543 
               
               
                 Hs.111779 
                 secreted protein, acidic, 
                 182120 
                 NP_003109 
               
               
                   
                 cysteine-rich (osleonectin) 
               
               
                 Hs.119129 
                 collagen, type IV, alpha 1 
                 120130 
                 NP_001836 
               
               
                 Hs.119571 
                 collagen, type III, alpha 1 
                 120180 
                 NP_000081 
               
               
                   
                 (Ehlers-Danlos syndrome type 
               
               
                   
                 IV, autosomal dominant) 
               
               
                 Hs.135084 
                 cystatin C (amyloid angiopathy 
                 604312 
                 NP_000090 
               
               
                   
                 and cerebral hemorrhage) 
               
               
                 Hs.136414 
                 UDP-GlcNAc:betaGal beta-1,3- 
                   
                 NP_114436 
               
               
                   
                 N- 
               
               
                   
                 acetylglucosaminyltransferase 5 
               
               
                 Hs.151738 
                 matrix metalloproteinase 9 
                 120361 
                 NP_004985 
               
               
                   
                 (gelatinase B, 92 kDa 
               
               
                   
                 gelatinase, 92 kDa type IV 
               
               
                   
                 collagenase) 
               
               
                 Hs.159263 
                 collagen, type VI, alpha 2 
                 120240 
                 NP_001840 
               
               
                 Hs.169401 
                 apolipoprotein E 
                 107741 
                 NP_000032 
               
               
                 Hs.172928 
                 collagen, type I, alpha 1 
                 120150 
                 NP_000079 
               
               
                 Hs.1735 
                 inhibin, beta B (activin AB beta 
                 147390 
                 NP_002184 
               
               
                   
                 polypeptide) 
               
               
                 Hs.179573 
                 TEM40, COL1A2 all polyA; 
                 120160 
                 NP_000080 
               
               
                   
                 involved in tissue remodeling 
               
               
                 Hs.180324 
                 insulin-like growth factor 
                 146734 
               
               
                   
                 binding protein 5 
               
               
                 Hs.18069 
                 legumain 
                 602620 
                 NP_005597 
               
               
                 Hs.211573 
                 heparan sulfate proteoglycan 2 
                 142461 
                 NP_005520 
               
               
                   
                 (perlecan) 
               
               
                 Hs.25590 
                 stanniocalcin 1 
                 601185 
                 NP_003146 
               
               
                 Hs.268571 
                 apolipoprotein C-I 
                 107710 
               
               
                 Hs.321231 
                 UDP-Gal:betaGlcNAc beta 1,4- 
                 604014 
                 NP_003770 
               
               
                   
                 galactosyltransferase, 
               
               
                   
                 polypeptide 3 
               
               
                 Hs.365706 
                 matrix Gla protein 
                 154870 
                 NP_000891 
               
               
                 Hs.367653 
                 hypothetical protein FLJ22329 
               
               
                 Hs.69954 
                 laminin, gamma 3 
                 604349 
                 NP_006050 
               
               
                 Hs.73817 
                 chemokine (C-C motif) ligand 3 
                 182283 
                 NP_002974 
               
               
                 Hs.75111 
                 protease, serine, 11 (IGF 
                 602194 
                 NP_002766 
               
               
                   
                 binding) 
               
               
                 Hs.75445 
                 SPARC-like 1 (mast9, hevin) 
                 606041 
                 NP_004675 
               
               
                 Hs.75617 
                 collagen, type IV, alpha 2 
                 120090 
                 NP_001837 
               
               
                 Hs.75736 
                 apolipoprotein D 
                 107740 
                 NP_001638 
               
               
                 Hs.7718 
                 hypothetical protein FLJ22678 
                   
                 NP_078812 
               
               
                 Hs.77326 
                 insulin-like growth factor 
                 146732 
                 NP_000589 
               
               
                   
                 binding protein 3 
               
               
                 Hs.78575 
                 prosaposin (variant Gaucher 
                 176801 
                 NP_002769 
               
               
                   
                 disease and variant 
               
               
                   
                 metachromatic 
               
               
                   
                 leukodystrophy) 
               
               
                 Hs.78672 
                 laminin, alpha 4 
                 600133 
                 NP_002281 
               
               
                 Hs.82085 
                 serine (or cysteine) proteinase 
                 173360 
                 NP_000593 
               
               
                   
                 inhibitor, clade E (nexin, 
               
               
                   
                 plasminogen activator inhibitor 
               
               
                   
                 type 1), member 1 
               
               
                 Hs.821 
                 biglycan 
                 301870 
                 NP_001702 
               
               
                 Hs.83169 
                 matrix metalloproteinase 1 
                 120353 
                 NP_002412 
               
               
                   
                 (interstitial collagenase) 
               
               
                 Hs.90107 
                 adhesion regulating molecule 1 
                   
                 NP_008933 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 22 
               
             
             
               
                   
               
               
                 Brain tumor markers unsorted 
               
             
          
           
               
                 Unigene ID 
                 Function 
                 OMIMID 
                 Protein 
               
               
                   
               
               
                 Hs.105850 
                 KIAA0404 protein 
                   
                 BAA23700 
               
               
                 Hs.107125 
                 plasmalemma vesicle 
                   
                 NP_112600 
               
               
                   
                 associated protein 
               
               
                 Hs.1103 
                 transforming growth factor, 
                 190180 
                 NP_000651 
               
               
                   
                 beta 1 (Camurati-Engelmann 
               
               
                   
                 disease) 
               
               
                 Hs.110443 
                 hypothetical protein FLJ22215 
                   
                 NP_073745 
               
               
                 Hs.110802 
                 von Willebrand factor 
                 193400 
                 NP_000543 
               
               
                 Hs.111611 
                 ribosomal protein L27 
                 607526 
                 NP_000979 
               
               
                 Hs.111779 
                 secreted protein, acidic, 
                 182120 
                 NP_003109 
               
               
                   
                 cysteine-rich (osteonectin) 
               
               
                 Hs.11607 
                 hypothetical protein FLJ32205 
                   
                 NP_689774 
               
               
                 Hs.119129 
                 collagen, type IV, alpha 1 
                 120130 
                 NP_001836 
               
               
                 Hs.119571 
                 collagen, type III, alpha 1 
                 120180 
                 NP_000081 
               
               
                   
                 (Ehlers-Danlos syndrome type 
               
               
                   
                 IV, autosomal dominant) 
               
               
                 Hs.121849 
                 microtubule-associated 
                   
                 NP_073729 
               
               
                   
                 protein 1 light chain 3 beta 
               
               
                 Hs.125359 
                 TEM13, Thy-1 cell surface 
                 188230 
                 NP_006279 
               
               
                   
                 antigen 
               
               
                 Hs.127824 
                 ESTs, Weakly similar to 
               
               
                   
                 CA28_HUMAN Collagen 
               
               
                   
                 alpha 2(VIII) chain 
               
               
                   
                 (Endothelial collagen) 
               
               
                   
                 [ H. sapiens ] 
               
               
                 Hs.129673 
                 eukaryotic translation initiation 
                 602641 
                 NP_001407 
               
               
                   
                 factor 4A, isoform 1 
               
               
                 Hs.135084 
                 cystatin C (amyloid 
                 604312 
                 NP_000090 
               
               
                   
                 angiopathy and cerebral 
               
               
                   
                 hemorrhage) 
               
               
                 Hs.136414 
                 UDP-GlcNAc:betaGal beta- 
                   
                 NP_114436 
               
               
                   
                 1,3-N- 
               
               
                   
                 acetylglucosaminyltransferase 5 
               
               
                 Hs.137574 
                 coagulation factor II 
                 602779 
                 NP_003941 
               
               
                   
                 (thrombin) receptor-like 3 
               
               
                 Hs.143897 
                 dysferlin, limb girdle muscular 
                 603009 
                 NP_003485 
               
               
                   
                 dystrophy 2B (autosomal 
               
               
                   
                 recessive) 
               
               
                 Hs.148932 
                 sema domain, 
                   
                 NP_115484 
               
               
                   
                 transmembrane domain (TM), 
               
               
                   
                 and cytoplasmic domain, 
               
               
                   
                 (semaphorin) 6B 
               
               
                 Hs.149098 
                 smoothelin 
                 602127 
                 NP_599031 
               
               
                 Hs.149609 
                 integrin, alpha 5 (fibronectin 
                 135620 
                 NP_002196 
               
               
                   
                 receptor, alpha polypeptide) 
               
               
                 Hs.151738 
                 matrix metalloproteinase 9 
                 120361 
                 NP_004985 
               
               
                   
                 (gelatinase B, 92 kDa 
               
               
                   
                 gelatinase, 92 kDa type IV 
               
               
                   
                 collagenase) 
               
               
                 Hs.155396 
                 nuclear factor (erythroid- 
                 600492 
                 NP_006155 
               
               
                   
                 derived 2)-like 2 
               
               
                 Hs.159263 
                 collagen, type VI, alpha 2 
                 120240 
                 NP_001840 
               
               
                 Hs.160958 
                 CDC37 cell division cycle 37 
                 605065 
                 NP_008996 
               
               
                   
                 homolog ( S. cerevisiae ) 
               
               
                 Hs.166254 
                 likely ortholog of rat vacuole 
                   
                 NP_112200 
               
               
                   
                 membrane protein 1 
               
               
                 Hs.169401 
                 apolipoprotein E 
                 107741 
                 NP_000032 
               
               
                 Hs.172813 
                 Rho guanine nucleotide 
                 605477 
                 NP_663788 
               
               
                   
                 exchange factor (GEF) 7 
               
               
                 Hs.172928 
                 collagen, type I, alpha 1 
                 120150 
                 NP_000079 
               
               
                 Hs.1735 
                 inhibin, beta B (activin AB 
                 147390 
                 NP_002184 
               
               
                   
                 beta polypeptide) 
               
               
                 Hs.179573 
                 TEM40, COL1A2 alt polyA; 
                 120160 
                 NP_000080 
               
               
                   
                 involved in tissue remodeling 
               
               
                 Hs.180324 
                 insulin-like growth factor 
                 146734 
               
               
                   
                 binding protein 5 
               
               
                 Hs.18069 
                 legumain 
                 602620 
                 NP_005597 
               
               
                 Hs.180920 
                 ribosomal protein S9 
                 603631 
               
               
                 Hs.1827 
                 nerve growth factor receptor 
                 162010 
                 NP_002498 
               
               
                   
                 (TNFR superfamily, member 
               
               
                   
                 16) 
               
               
                 Hs.185973 
                 degenerative spermatocyte 
                   
                 NP_003667 
               
               
                   
                 homolog, lipid desaturase 
               
               
                   
                 ( Drosophila ) 
               
               
                 Hs.195727 
                 TEM1, endosialin 
                 606064 
                 NP_065137 
               
               
                 Hs.197298 
                 NS1-binding protein 
                   
                 AAG43485 
               
               
                 Hs.202833 
                 heme oxygenase (decycling) 1 
                 141250 
                 NP_002124 
               
               
                 Hs.20976 
                   Homo sapiens  cDNA 
               
               
                   
                 FLJ34888 fis, clone 
               
               
                   
                 NT2NE2017332 
               
               
                 Hs.211573 
                 heparan sulfate proteoglycan 
                 142461 
                 NP_005520 
               
               
                   
                 2 (perlecan) 
               
               
                 Hs.211600 
                 tumor necrosis factor, alpha- 
                 191163 
                 NP_006281 
               
               
                   
                 induced protein 3 
               
               
                 Hs.217493 
                 annexin A2 
                 151740 
               
               
                 Hs.23016 
                 G protein-coupled receptor 
               
               
                 Hs.2340 
                 junction plakoglobin 
                 173325 
                 NP_002221 
               
               
                 Hs.236516 
                 C-type (calcium dependent, 
                   
                 NP_055173 
               
               
                   
                 carbohydrate-recognition 
               
               
                   
                 domain) lectin, superfamily 
               
               
                   
                 member 9 
               
               
                 Hs.2399 
                 matrix metalloproteinase 14 
                 600754 
                 NP_004986 
               
               
                   
                 (membrane-inserted) 
               
               
                 Hs.25450 
                 solute carrier family 29 
                 602193 
                 NP_004946 
               
               
                   
                 (nucleoside transporters). 
               
               
                   
                 member 1 
               
               
                 Hs.25590 
                 stanniocalcin 1 
                 601185 
                 NP_003146 
               
               
                 Hs.268571 
                 apolipoprotein C-I 
                 107710 
               
               
                 Hs.274184 
                 transcription factor binding to 
                 314310 
                 NP_006512 
               
               
                   
                 IGHM enhancer 3 
               
               
                 Hs.274453 
                 likely ortholog of mouse 
                   
                 NP_060081 
               
               
                   
                 embryonic epithelial gene 1 
               
               
                 Hs.277477 
                 major histocompatibility 
                 142840 
                 NP_002108 
               
               
                   
                 complex, class I, C 
               
               
                 Hs.27836 
                 likely ortholog of mouse 
                   
                 NP_073734 
               
               
                   
                 fibronectin type III repeat 
               
               
                   
                 containing protein 1 
               
               
                 Hs.285814 
                 sprouty homolog 4 
                   
                 AAK00653 
               
               
                   
                 ( Drosophila ) 
               
               
                 Hs.286035 
                 myosin XVB, pseudogene 
               
               
                 Hs.288203 
                   Homo sapiens , clone 
               
               
                   
                 IMAGE: 4845226, mRNA 
               
               
                 Hs.29797 
                 ribosomal protein L10 
                 312173 
                 NP_115617 
               
               
                 Hs.299257 
                 ESTs, Weakly similar to 
               
               
                   
                 hypothetical protein FLJ20489 
               
               
                   
                 [ Homo sapiens ] [ H. sapiens ] 
               
               
                 Hs.301685 
                 KIAA0620 protein 
                   
                 BAA31595 
               
               
                 Hs.302741 
                   Homo sapiens  mRNA full 
               
               
                   
                 length insert cDNA clone 
               
               
                   
                 EUROIMAGE 50374 
               
               
                 Hs.318751 
                 ESTs, Weakly similar to 
               
               
                   
                 T21371 hypothetical protein 
               
               
                   
                 F25H8.3 -  Caenorhabditis   
               
               
                   
                   elegans  [ C. elegans ] 
               
               
                 Hs.321231 
                 UDP-Gal:betaGlcNAc beta 
                 604014 
                 NP_003770 
               
               
                   
                 1,4-galactosyltransferase, 
               
               
                   
                 polypeptide 3 
               
               
                 Hs.327412 
                 TEM15, COL3A1,  Homo   
               
               
                   
                   sapiens  clone FLC1492 
               
               
                   
                 PRO3121 mRNA, complete 
               
               
                   
                 cds 
               
               
                 Hs.332173 
                 transducin-like enhancer of 
                 601041 
                 NP_003251 
               
               
                   
                 split 2 (E(sp1) homolog, 
               
               
                   
                   Drosophila ) 
               
               
                 Hs.337986 
                 hypothetical protein MGC4677 
                   
                 NP_443103 
               
               
                 Hs.339283 
                 endoplasmic reticulum 
               
               
                   
                 associated protein 140 kDa 
               
               
                 Hs.34516 
                 ceramide kinase 
                   
                 NP_073603 
               
               
                 Hs.350065 
                 hypothetical protein FLJ30634 
                   
                 NP_694559 
               
               
                 Hs.351928 
                   Homo sapiens  mRNA full 
               
               
                   
                 length insert cDNA clone 
               
               
                   
                 EUROIMAGE 1977059 
               
               
                 Hs.352535 
                 KIAA0943 protein 
                   
                 BAA76787 
               
               
                 Hs.352949 
                 ESTs, Weakly similar to 
               
               
                   
                 hypothetical protein FLJ20489 
               
               
                   
                 [ Homo sapiens ] [ H. sapiens ] 
               
               
                 Hs.356096 
                 ESTs, Highly similar to 
               
               
                   
                 hypothetical protein FLJ10350 
               
               
                   
                 [ Homo sapiens ] [ H. sapiens ] 
               
               
                 Hs.363027 
                   Homo sapiens  cDNA 
               
               
                   
                 FLJ39848 fis, clone 
               
               
                   
                 SPLEN2014669 
               
               
                 Hs.365706 
                 matrix Gla protein 
                 154870 
                 NP_000891 
               
               
                 Hs.367653 
                 hypothetical protein FLJ22329 
               
               
                 Hs.374415 
                 ESTs 
               
               
                 Hs.380983 
                 ESTs, Highly similar to 
               
               
                   
                 ITB1_HUMAN Integrin beta-1 
               
               
                   
                 precursor (Fibronectin 
               
               
                   
                 receptor beta subunit) (CD29) 
               
               
                   
                 (Integrin VLA-4 beta subunit) 
               
               
                   
                 [ H. sapiens ] 
               
               
                 Hs.400429 
                 ESTs 
               
               
                 Hs.401975 
                 ESTs, Weakly similar to 
               
               
                   
                 T17346 hypothetical protein 
               
               
                   
                 DKFZp586O1624.1 - human 
               
               
                   
                 (fragment) [ H. sapiens ] 
               
               
                 Hs.61661 
                 F-box only protein 32 
                 606604 
                 NP_478136 
               
               
                 Hs.62192 
                 coagulation factor III 
                 134390 
                 NP_001984 
               
               
                   
                 (thromboplastin, tissue factor) 
               
               
                 Hs.65238 
                 ring finger protein 40 
                   
                 NP_055586 
               
               
                 Hs.6657 
                 Hermansky-Pudlak syndrome 4 
                 606682 
                 BAB33337 
               
               
                 Hs.69954 
                 laminin, gamma 3 
                 604349 
                 NP_006050 
               
               
                 Hs.73798 
                 macrophage migration 
                 153620 
                 NP_002406 
               
               
                   
                 inhibitory factor (glycosylation- 
               
               
                   
                 inhibiting factor) 
               
               
                 Hs.73817 
                 chemokine (C-C motif) ligand 3 
                 182283 
                 NP_002974 
               
               
                 Hs.74602 
                 aquaporin 1 (channel-forming 
                 110450 
                 AAH22486 
               
               
                   
                 integral protein, 28 kDa) 
               
               
                 Hs.75061 
                 MARCKS-like protein 
                 602940 
                 NP_075385 
               
               
                 Hs.75111 
                 protease, serine, 11 (IGF 
                 602194 
                 NP_002766 
               
               
                   
                 binding) 
               
               
                 Hs.75445 
                 SPARC-like 1 (mast9, hevin) 
                 606041 
                 NP_004675 
               
               
                 Hs.75617 
                 collagen, type IV, alpha 2 
                 120090 
                 NP_001837 
               
               
                 Hs.75721 
                 profilin 1 
                 176610 
                 NP_005013 
               
               
                 Hs.75736 
                 apolipoprotein D 
                 107740 
                 NP_001638 
               
               
                 Hs.7718 
                 hypothetical protein FLJ22678 
                   
                 NP_078812 
               
               
                 Hs.77326 
                 insulin-like growth factor 
                 146732 
                 NP_000589 
               
               
                   
                 binding protein 3 
               
               
                 Hs.77573 
                 uridine phosphorylase 
                 191730 
                 NP_003355 
               
               
                 Hs.77886 
                 lamin A/C 
                 150330 
                 NP_005563 
               
               
                 Hs.77961 
                 major histocompatibility 
                 142830 
                 NP_005505 
               
               
                   
                 complex, class I, B 
               
               
                 Hs.78575 
                 prosaposin (variant Gaucher 
                 176801 
                 NP_002769 
               
               
                   
                 disease and variant 
               
               
                   
                 metachromatic 
               
               
                   
                 leukodystrophy) 
               
               
                 Hs.78672 
                 laminin, alpha 4 
                 600133 
                 NP_002281 
               
               
                 Hs.79356 
                 Lysosomal-associated 
                 601476 
                 NP_006753 
               
               
                   
                 multispanning membrane 
               
               
                   
                 protein-5 
               
               
                 Hs.82002 
                 endothelin receptor type B 
                 131244 
                 NP_000106 
               
               
                 Hs.82085 
                 serine (or cysteine) proteinase 
                 173360 
                 NP_000593 
               
               
                   
                 inhibitor, clade E (nexin, 
               
               
                   
                 plasminogen activator 
               
               
                   
                 inhibitor type 1), member 1 
               
               
                 Hs.821 
                 biglycan 
                 301870 
                 NP_001702 
               
               
                 Hs.83169 
                 matrix metalloproteinase 1 
                 120353 
                 NP_002412 
               
               
                   
                 (interstitial collagenase) 
               
               
                 Hs.83384 
                 S100 calcium binding protein, 
                 176990 
                 NP_006263 
               
               
                   
                 beta (neural) 
               
               
                 Hs.84063 
                   Homo sapiens  cDNA: 
               
               
                   
                 FLJ23507 fis, clone 
               
               
                   
                 LNG03128 
               
               
                 Hs.89695 
                 insulin receptor 
                 147670 
                 NP_000199 
               
               
                 Hs.90107 
                 adhesion regulating molecule 1 
                   
                 NP_008933 
               
               
                 Hs.97199 
                 complement component 1, q 
                 120577 
                 NP_036204 
               
               
                   
                 subcomponent, receptor 1