Abstract:
The present invention provides novel isolated and purified nucleic acid (RNA or DNA) encoding, or complementary to, a canine PepT1 (cPepT1). The present invention also provide a method for determining canine PepT1-transportability of a peptide, or method for determining a peptide with beneficial nutritional property in an animal. The present invention further provides a dietary composition for an animal comprising a peptide identified by the method described above.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
     This application claims the benefit of U.S. Provisional Application Ser. No. 60/273,263, filed Mar. 2, 2001, under 35 U.S.C. 119(e) and U.S. Provisional Application Ser. No. 60/344,088, filed Dec. 26, 2001, under 35 U.S.C. 119(e). 
    
    
     BACKGROUND OF THE INVENTION 
     In dogs, it is thought that the ability to absorb essential amino acids such as tryptophan and leucine may be limiting to cellular metabolism. Recent research designed to characterize the amino acid absorption capacity of the brush border (lumen facing) membranes of dog enterocytes suggests that peptide absorption may be particularly important given the relatively low amount of free amino acid transport capacity that was observed. Buddington R K, Paulsen D B. Development of the Canine and Feline Gastrointestinal Tract. In: Reinhart G A, Carey D P, eds.  Recent Advances in Canine and Feline Nutrition, Vol. II:  1998  Iams Nutrition Symposium Proceedings . Wilmington: Orange Frazer Press, 1998; 195-215. Data collected from studies designed to understand the quantitative importance of free versus peptide amino acids in other monogastric animals strongly indicates that peptide-bound amino acids account for the majority of amino acids absorbed by enterocytes from the intestinal lumen (Matthews, D M.  Protein Absorption, Development and Present State of the Subject , New York: Wiley-Liss, 1991.) and that the rate of peptide-derived amino acid absorption is faster than that by equivalent amounts of free amino acids. Ohkohchi N, Andoh T, Ohi R, Mori S. Defined formula diets alter characteristics of the intestinal transport of amino acid and peptide in growing rats.  J Pediatr Gastroenterol Nutr  1990 May; 10(4):490-6. 
     Two types of peptide transporters have been cloned from monogastric animals. Liang R, Fei Y J, Prasad P D, Ramamoorthy S, Han H, Yang-Feng T L, Hediger M A, Ganapathy V, Leibach F H. Human intestinal H+/peptide cotransporter. Cloning, functional expression, and chromosomal localization.  J Biol Chem  1995 Mar. 24; 270(12):6456-63. Liu W, Liang R, Ramamoorthy S, Fei Y J, Ganapathy M E, Hediger M A, Ganapathy V, Leibach F H. Molecular cloning of PEPT 2, a new member of the H+/peptide cotransporter family, from human kidney.  Biochim Biophys Acta  1995 May 4; 1235(2):461-6. PepT1 is an H + -dependent, low-affinity (mM), high-velocity, transporter that is predominately localized primarily to the brush border membranes of mature enterocytes of intestinal villi. PepT2 is an H + -dependent, high-affinity (μM), low-velocity, transporter that is expressed in the greatest abundance in the apical membranes of renal proximal tubular epithelial cells. An important feature of the peptide transporters is their ability to recognize and transport most di- and tripeptides, albeit with a range of relative affinities for different peptides. In addition, both transporters recognize the β-lactam antibiotics, and carboxyl-terminal modified free amino acids. The physiologic functions of these transporters are thought to be to absorb di- and tripeptides from the digesta and from the blood, respectively. Although molecular evidence has not been acquired, there is strong biochemical evidence for a different peptide transport protein that functions in the basolateral membrane of these cells. Saito H, Inui K I. Dipeptide transporters in apical and basolateral membranes of the human intestinal cell line Caco-2 . Am J Physiol  1993 August; 265(2 Pt 1):G289-94. Thwaites D T, Brown C D, Hirst B H, Simmons N L. Transepithelial glycylsarcosine transport in intestinal Caco-2 cells mediated by the expression of H + -coupled carriers at both the apical and basal membranes.  J Biol Chem  1993 April 15; 268(11):7640-2. 
     Research with Caco-2 cells indicates that PepT1 transporter mRNA, protein, and activity increases in a manner consistent with a direct effect of increased extracellular substrate concentrations. Walker D, Thwaites D T, Simmons N L, Gilbert H J, Hirst B H. Substrate upregulation of the human small intestinal peptide transporter, hPepT1.  J Physiol  1998 Mar. 15; 507(Pt 3):697-706. In contrast to mRNAs for essential amino acid transporters, intestinal studies show that the expression of peptide transporter mRNA increases in response to increased dietary protein. Erickson R H, Gum J R Jr, Lindstrom M M, McKean D, Kim Y S. Regional expression and dietary regulation of rat small intestinal peptide and amino acid transporter mRNAs.  Biochem Biophys Res Commun  1995 Nov. 2; 216(1):249-57. Similarly, expression in intestinal mucosa of PepT1 mRNA and protein increases in response to tissue trauma, whereas the MRNA for essential amino acid transporters decreases. Tanaka H, Miyamoto K I, Morita K, Haga H, Segawa H, Shiraga T, Fujioka A, Kuoda T, Taketani Y, Hisano S, Fukui Y, Kitagawa K, Takeda E. Regulation of the PepT1 peptide transporter in the rat small intestine in response to 5-fluorouracil-induced injury.  Gastroenterology  1998 April; 114(4):714-23. 
     Few studies have been conducted to evaluate the potential for the dog to absorb quantitatively significant amounts of essential amino acids in the form of small peptides, and whether this capacity can be regulated by substrate supply. Accordingly, there is still a need to evaluate the potential for the absorption of peptide-bound leucine and tryptophan by putative canine peptide transporters. It would thus be desirable to provide the nucleic acid sequence encoding canine PepT1. It would also be desirable to provide mRNA transcripts corresponding to cPepT1. It would further be desirable to characterize the function of cPepT1 by GlySar uptake and identify di- and tripeptides well recognized by cPepT1, as well as characterize the effect of supplemental peptide substrate on the transport capacity of canine PepT1 (cPepT1). 
     SUMMARY OF THE INVENTION 
     The present invention provides novel isolated and purified nucleic acids (RNA or DNA) encoding, or complementary to, canine PepT1 (cPepT1). The nucleic acid may be SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:20 or may be a nucleic acid that hybridizes under moderate or stringent hybridization conditions to any of these sequences. Also provided are peptides encoded by these nucleic acids, such as SEQ ID NO: 13 or SEQ ID NO:21. 
     The present invention also provides a method for determining canine PepT1-transportability of a peptide, or method for determining a peptide with beneficial nutritional property in an animal, comprising providing an immortalized kidney distal tubule epithelial (Madin-Darby Canine Kidney (MDCK)) cell and a peptide having 2 to 10 amino acids, and determining the amount of the peptide transported into the cell, wherein the amount correlates with the canine PepT1-transportability of the peptide. A peptide with beneficial nutritional properties in an animal is a peptide that contains at least one essential amino acid that is absorbed at a rate higher than the rate of the amino acid if it were free rather than in a peptide-bound form. The peptide may be a dipeptide, tripeptide, or tetrapeptide such as, for example, GlySar, GlyGly, AlaHis, β-AlaHis (carnosine), GlnGln, GlyMet, LeuMet, LeuTrp, MetLeu, MetMet, MetPhe, MetPro, TrpLeu, TrpTrp, GlnGlu, MetGlu, MetLys, TrpGly, MetGlyMetMet (SEQ ID NO:10), TrpGlyGly, LeuArg, ArgLeu, GlyLeu, or ArgTrp. The cell used in the method may be in medium at a pH of between about 5 and 8; or at a pH of about 5.5 to 7.5, or even at about 6 to 6.5. The peptide may be present at a concentration of about 10 nm to about 50 mM. 
     The characterization of GlySar uptake by immortalized MDCK cells demonstrates that MDCK cells express PepT1-like activity, confirming detection of PepT1 MRNA expression by MDCK cells and the use of MDCK cells as a model to characterize the biochemical function of canine PepT1. 
     The cPepT1 of the present invention is also capable of recognizing a variety of di- and tripeptides, including those that contain the essential amino acids leucine and tryptophan, considered to be of especial importance to canine nutrition. In addition, H + -dependent peptide transport in cultured MDCK cells can be stimulated by at least two of PepT1 substrates, GlySar and carnosine. Moreover, H + -dependent uptake of GlySar by MDCK is sensitive to nutrient deprivation and Insulin-like Growth factor I (IGF-I). 
     The present invention further provides a dietary composition with improved nutritional benefit for an animal comprising at least one peptide identified by the method described above. 
     The present invention provides a process for altering the absorption of essential amino acids in an animal, such as a dog, comprising the steps of feeding the animal a diet containing the dietary composition described above; and maintaining the animal on the diet for a sufficient period of time to allow the composition to be absorbed by the digestive system of the animal. The diet may comprise about 20 to about 30% crude protein, about 10 to about 20% fat, and about 3 to about 10% dietary fiber. 
     As used herein, the term “cPepT1” includes variants or biologically active or inactive fragments of this transport protein. A “variant” of the polypeptide is a cPepT1 protein that is not completely identical to a native cPepT1 protein. A variant cPepT1 protein can be obtained by altering the amino acid sequence by insertion, deletion or substitution of one or more amino acid. The amino acid sequence of the protein is modified, for example by substitution, to create a polypeptide having substantially the same or improved qualities as compared to the native polypeptide. The substitution may be a conserved substitution. A “conserved substitution” is a substitution of an amino acid with another amino acid having a similar side chain. A conserved substitution would be a substitution with an amino acid that makes the smallest change possible in the charge of the amino acid or size of the side chain of the amino acid (alternatively, in the size, charge or kind of chemical group within the side chain) such that the overall peptide retains its spacial conformation but has altered biological activity. For example, common conserved changes might be Asp to Glu, Asn or Gln; His to Lys, Arg or Phe; Asn to Gln, Asp or Glu and Ser to Cys, Thr or Gly. Alanine is commonly used to substitute for other amino acids. The 20 common amino acids can be grouped as follows: alanine, valine, leucine, isoleucine, proline, phenylalanine, tryptophan and methionine having nonpolar side chains; glycine, serine, threonine, cystine, tyrosine, asparagine and glutamine having uncharged polar side chains; aspartate and glutamate having acidic side chains; and lysine, arginine, and histidine having basic side chains. Stryer, L.  Biochemistry  (2d edition) W. H. Freeman and Co. San Francisco (1981), p. 14-15; Lehninger, A.  Biochemistry  (2d ed., 1975), p. 73-75. It is known to those of skill in the art that transport of other, less common, amino acids such as hydroxylysine, or derivatives of any one of the 20 common amino acids listed above would also be within the scope of this invention. 
     It is known that variant polypeptides can be obtained based on substituting certain amino acids for other amino acids in the polypeptide structure in order to modify or improve biological activity. For example, through substitution of alternative amino acids, small conformational changes may be conferred upon a polypeptide that result in increased bioactivity. Alternatively, amino acid substitutions in certain polypeptides may be used to provide residues that may then be linked to other molecules to provide peptide-molecule conjugates that retain sufficient properties of the starting polypeptide to be useful for other purposes. 
     One can use the hydropathic index of amino acids in conferring interactive biological function on a polypeptide, wherein it is found that certain amino acids may be substituted for other amino acids having similar hydropathic indices and still retain a similar biological activity. Alternatively, substitution of like amino acids may be made on the basis of hydrophilicity. It is noted that substitutions can be made based on the hydrophilicity assigned to each amino acid. In using either the hydrophilicity index or hydropathic index, which assigns values to each amino acid, it is preferred to conduct substitutions of amino acids where these values are ±2, with ±1 being particularly preferred, and those with in ±0.5 being the most preferred substitutions. 
     The variant cPepT1 protein comprises at least seven amino acid residues, preferably about 20 to about 700 residues, and more preferably about 50 to about 700 residues, wherein the variant cPepT1 protein has at least 50%, preferably at least about 80%, and more preferably at least about 90% but less than 100%, contiguous amino acid sequence homology or identity to the amino acid sequence of a corresponding native cPepT1 protein. 
     The amino acid sequence of the variant cPepT1 protein corresponds essentially to the native cPepT1 protein amino acid sequence. As used herein “correspond essentially to” refers to a polypeptide sequence that will elicit an absorption value substantially the same as the absorption stimulated by native cPepT1 protein. Such absorption may be at least 60% of the level generated by native cPepT1 protein, and may even be at least 80% of the level generated by native cPepT1 protein. 
     A variant of the invention may include amino acid residues not present in the corresponding native cPepT1 protein, or may include deletions relative to the corresponding native cPepT1 protein. A variant may also be a truncated “fragment” as compared to the corresponding native cPepT1 protein, i.e., only a portion of a full-length protein. cPepT1 protein variants also include peptides having at least one D-amino acid. 
     The cPepT1 protein of the present invention may be expressed from an isolated nucleic acid (DNA or RNA) sequence encoding the cPepT1 protein. Amino acid changes from the native to the variant cPepT1 protein may be achieved by changing the codons of the corresponding nucleic acid sequence. “Recombinant” is defined as a peptide or nucleic acid produced by the processes of genetic engineering. It should be noted that it is well-known in the art that, due to the redundancy in the genetic code, individual nucleotides can be readily exchanged in a codon, and still result in an identical amino acid sequence. The terms “protein,” “peptide” and “polypeptide” are used interchangeably herein. 
    
    
     BRIEF DESCRIPTION OF THE FIGURES 
     FIG. 1 is a photograph of an electrophoresis gel showing the partial length canine PepT1 cDNA reaction products generated by reverse transcription-polymerase chain reaction (RT-PCR) methodology. Partial length canine PepT1 (cPepT1, about 783 bp) cDNAs were generated by reverse transcription-polymerase chain reaction (RT-PCR) methodology. RT-PCR reaction products were generated using mRNA isolated from canine jejunal epithelium and two different PCR primer sets. Gel contents are as follows: lane 1, 1 Kb molecular weight DNA ladder; lane 2, negative control PCR reaction (lacks Taq polymerase); lane 3, PCR reaction products using primer set 4 (corresponding to base pairs 83 to 863 of rabbit PepT1); lane 4, PCR reaction products using primer set 10˜780 bp cDNA product using primer set 10 (corresponding to base pairs 85 to 861 of rabbit PepT1). Note the reaction products in lanes 3 and 4 of about 780 base pairs. 
     FIGS. 2A and 2B are photographs of agarose gels showing the representative results of restriction analyses of pCR®II/cPepT1 plasmids generated by TA-cloning of primer set 4derived RT-PCR cDNA. Restriction analyses of pCR®II/cPepT1 plasmids generated by TA-cloning of primer set 4-derived RT-PCR cDNA are shown in these figures. Data are representative of four cDNA-containing plasmids from a total of fifty-six “positive” bacterial colonies selected by blue/white screening. TA-clones were amplified, pCR®II/cDNA vectors isolated, and Xho I and Kpn I endonucleases restriction products size-separated through 1.2% agarose gels. FIG. 2A is a photograph of an agarose gel showing representative results of the PCR-based analyses of TA-clone 26. In particular, analyses of pCR®II/cPepT1-26 (TA-clone 26) are shown; lane 1, 1 DNA size standard; lane 2, minus endonuclease-restriction control (uncut pCR®II plasmid); lane 3, positive restriction control (Xho I restriction of empty pCR®II vector); lane 4, uncut pCR®II/cPepT1-26 (Clone 26); lane 5, Xho I- and Kpn I-restricted Clone 26. Note that empty pCR®II vector is 3.9 kb in size and that lane 5 contains a product of about 780 bp. FIG. 2B is a photograph of an agarose gel showing representative results of the PCR-based analyses of TA-clone 4 and 6. In particular, analyses of TA-clone 4 and 6 are shown; lane 1, DNA size standard; lane 2, uncut pCR®II/cPepT1-4 (TA-clone 4); lane 3, Xho I- and Kpn I-restricted pCR®II/cPepT1-4; lane 4, uncut pCR®II/cPepT1-6 (TA-clone 6); lane 5, Xho I- and Kpn I-restricted pCR®II/cPepT1-6. Note that empty pCR®II vector is 3.9 kb in size and that lane 3 does not contain a product of about 780 bp, whereas lane 5 does. 
     FIGS. 3A and 3B are photographs showing the representative results of Northern blot identification of cPepT1 mRNA expression by canine tissues and MDCK cells using canine intestinal epithelium-derived RT-PCR cDNA. Arrangement of RNA isolated from tissue or cell homogenates on both blots is as follows: lane 1, kidney (animal #1031A); lane 2, kidney (animal K-9-1); lane 3, MDCK cells; lane 4, jejunal epithelium (animal K-9-4). FIG. 3A is a photograph showing the Northern blot identification of A + RNA (3 μg/lane) that was hybridized with [ 32 P]-cPepT1-26 cDNA. FIG. 3B is a photograph showing the Northern blot identification of total RNA (20 μg/lane) that was hybridized with [ 32 P]-cPepT1-6 cDNA. 
     FIG. 4 is a photograph showing the representative results of Northern blot identification of cPepT1 mRNA expression in canine tissues using full-length rabbit PepT1 cDNA. Ten μg total RNA (lane 1) or 6 μg A + RNA (lanes 2 to 5) were isolated from liver and kidney tissues from three animals. Lane 1, liver (animal #1042A); lane 2, liver (animal #1008A); lane 3, kidney (animal #1008A); lane 4, liver (animal #1031A); lane 5, kidney (animal #1031A). 
     FIG. 5 is the partial-length nucleic acid sequence of canine PepT1 cDNA of the present invention that was cloned from MDCK cells (SEQ ID NO:9). The 381 base pairs of TA clone PepT1-6R-20 shares 79% homology to base pairs 259 to 640 of rabbit PepT1 (GenBank acc no. 473375). 
     FIG. 6 is a graph illustrating the influence of extracellular GlySar concentrations on GlySar uptake by confluent MDCK cells in pH 6.0 media. By graphical evaluation, an apparent K m  of about 4 mM was demonstrated. Each data point is the mean of 5 to 6 observations and all coefficients of variation were less than 15%. 
     FIG. 7 is a graph illustrating the protein content of MDCK cells cultured in DMEM or LHM. Values are the means±SD of protein content of wells (n=12) of MDCK cells after seeding at 60,000 or 120,000 cells/well, culture for 1 d in DMEM, and then culture in DMEM or LHM for 1, 2, 3, or 5 d (Days 2, 3, 4 and 6, respectively). Protein content was determined by the method of Lowry, using bovine serum albumin as the standard. 
     FIG. 8 is a graph illustrating GlySar (2.88 μM) uptake in pH 6.0 or pH 7.4 buffer by MDCK cells cultured in DMEM or LHM. Uptake was measured in the absence (pH 7.4) or presence (pH 6.0) of an extracellular-to-intracellular H +  gradient. 
     FIG. 9 is a graph illustrating H + -dependent [ 3 H]-GlySar (2.88 μM) uptake by MDCK cells cultured in DMEM or LHM. Values were calculated as the difference in GlySar uptake in the presence (pH 6.0 uptake buffer) and absence (pH 7.4 uptake buffer) of an extracellular-to-intercellular H +  proton gradient. 
     FIG. 10 is a graph illustrating pH-dependent GlySar uptake by MDCK cells seeded at 60,000 cells/well and cultured in LHM for 2 days. pH-dependent GlySar (2.88 uM) uptake by MDCK cells cultured with standard conditions. Values represent the H + -dependent GlySar uptake means±SD of wells (n=16) of MDCK cells, calculated as the difference from GlySar uptake in the presence of pH 6.0 or 7.4 buffers. 
     FIG. 11 is a graph illustrating the effect of time on GlySar uptake (100 μM) by MDCK cells. By-minute time course for GlySar (uptake by MDCK cells cultured with standard conditions. Mean±SD GlySar uptake wells of cells (n=6) were assayed at 3.75, 7.5, 15, 30, 60, or 120 min. 
     FIG. 12 is a graph illustrating the effect of GlySar concentration on MDCK cells seeded at 60K/well grown in LHM. The graph indicates the K m  characterization (1.0 mM) of H + -dependent GlySar uptake by MDCK cells. Each value represents the mean±SD uptake of GlySar by wells (n=8) of MDCK cells cultured using standard conditions. 
     FIG. 13 is a graph illustrating the inhibition of peptide uptake by MDCK cells with antibiotics. The mean±SD are the uptake of GlySar by wells (n=5-8) of MDCK cells in the absence or presence of GlySar (1 mM) Penicillin-G (3 mM), cefadroxil (30 μM), or cefadroxil (3 mM). 
     FIG. 14 is a graph illustrating the inhibition of peptide uptake by MDCK cells with Gly-containing peptides. The mean±SD uptake of GlySar by wells (n=7-8) of MDCK cells in the absence or presence of indicated competitor substrates (1 mM). 
     FIG. 15 is a graph illustrating the inhibition of 100 μM GlySar uptake by 1 mM TrpLeu, LeuTrp, Leu, or Trp in the absence (pH 7.5) and presence (no pH designation) of a proton gradient and 1 mM of indicated substrates. Values are the mean±SD uptake of GlySar by wells (n=7-8) of MDCK cells, 
     FIG. 16 is a graph illustrating the inhibition of 100 μM GlySar uptake by MDCK cells in the absence (pH 7.5) and presence (no pH designation) of a proton gradient and 1 mM of Trp-containing peptides. Values are the mean±SD uptake of GlySar by wells (n=7-8) of MDCK cells. 
     FIG. 17 is a graph illustrating the inhibition of 100 μM GlySar uptake by MDCK cells in the absence (pH 7.5) and presence (no pH designation) of a proton gradient and 100 μM of Trp-containing peptides. Values are the mean±SD uptake of GlySar by wells (n=8) of MDCK cells. 
     FIG. 18 is a graph illustrating the IC 50  inhibition of H + -dependent GlySar uptake by TrpLeu and TrpTrp. K, values were determined for inhibition of H + -dependent 100 μM GlySar uptake by MDCK cells in the presence of 0, 0.025, 0.1, 0.4, or 1.6 mM TrpTrp or TrpLeu. Values are the mean±SD uptake of GlySar by wells (n=6-8) of MDCK cells. 
     FIG. 19 is a graph illustrating substrate (10 mM) regulation of protein content of MDCK cells cultured in DMEM. In particular, the influence of 10 mM carnosine, glycylphenylalanine (GlyPhe), Phe, or Gly supplementation of DMEM on protein content of MDCK cells was measured. 
     FIG. 20 is a graph illustrating substrate (10 mM) regulation of GlySar uptake by MDCK cells cultured in DMEM. In particular, the influence of 10 mM carnosine, glycylphenylalanine (GlyPhe), Phe, or Gly supplementation of DMEM on H + -dependent uptake of [ 3 H]Glycylsarcosine (GlySar) by MDCK cells was measured. 
     FIG. 21 is a graph illustrating substrate (10 mM) regulation of protein content of MDCK cells cultured in DMEM. In particular, the influence of 10 mM glycylsarcosine (GlySar), glycylproline (GlyPro), glycylphenylalanine (GlyPhe), or carnosine of DMEM on protein content of MDCK cells was measured. 
     FIG. 22 is a graph illustrating substrate (10 mM) regulation of GlySar uptake by MDCK cells cultured in DMEM. In particular, the influence of 10 mM glycylsarcosine (GlySar), glycylproline (GlyPro), glycylphenylalanine (GlyPhe), or carnosine on H + -dependent uptake of [ 3 H]Glycylsarcosine (GlySar) by MDCK cells was measured. 
     FIG. 23 is a graph illustrating the influence of DMEM, nutrient depleted, dexamethasone (Dex), or insulin (ins) on H + -dependent uptake of [ 3 H]Glycylsarcosine (GlySar) by MDCK cells. 
     FIG. 24 is a graph illustrating influence of IGF-I on H + -dependent uptake of [ 3 H]Glycylsarcosine (GlySar) by MDCK cells. 
    
    
     DEFINITIONS 
     The term “gene” is used broadly to refer to any segment of nucleic acid associated with a biological function. Thus, genes include coding sequences and/or the regulatory sequences required for their expression. For example, gene refers to a nucleic acid fragment that expresses mRNA, functional RNA, or specific protein, including regulatory sequences. Genes also include nonexpressed DNA segments that, for example, form recognition sequences for other proteins. Genes can be obtained from a variety of sources, including cloning from a source of interest or synthesizing from known or predicted sequence information, and may include sequences designed to have desired parameters. 
     The term “native gene” refers to gene that is present in the genome of an untransformed cell. 
     “Naturally occurring” is used to describe an object that can be found in nature as distinct from being artificially produced by man. For example, a protein or nucleotide sequence present in an organism (including a virus), which can be isolated from a source in nature and which has not been intentionally modified by man in the laboratory, is naturally occurring. 
     A “marker gene” encodes a selectable or screenable trait. 
     The term “chimeric gene” refers to any gene that contains 1) DNA sequences, including regulatory and coding sequences, that are not found together in nature, or 2) sequences encoding parts of proteins not naturally adjoined, or 3) parts of promoters that are not naturally adjoined. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or comprise regulatory sequences and coding sequences derived from the same source, but arranged in a manner different from that found in nature. 
     A “transgene” refers to a gene that has been introduced into the genome by transformation and is stably maintained. Transgenes may include, for example, genes that are either heterologous or homologous to the genes of a particular cell to be transformed. Additionally, transgenes may comprise native genes inserted into a non-native organism, or chimeric genes. The term “endogenous gene” refers to a native gene in its natural location in the genome of an organism. A “foreign” gene refers to a gene not normally found in the host organism but that is introduced by gene transfer. 
     The terms “protein,” “peptide” and “polypeptide” are used interchangeably herein. 
     Expression cassettes will comprise the transcriptional initiation region of the invention linked to a nucleotide sequence of interest. Such an expression cassette is provided with a plurality of restriction sites for insertion of the gene of interest to be under the transcriptional regulation of the regulatory regions. The expression cassette may additionally contain selectable marker genes. 
     The transcriptional cassette will include in the 5′-3′ direction of transcription, a transcriptional and translational initiation region, a DNA sequence of interest, and a transcriptional and translational termination region. The termination region may be native with the transcriptional initiation region, may be native with the DNA sequence of interest, or may be derived from another source. 
     An oligonucleotide for use in probing or amplification reactions may be about 30 or fewer nucleotides in length (e.g., 9, 12, 15, 18, 20, 21 or 24, or any number between 9 and 30). Generally specific primers are upwards of 14 nucleotides in length For optimum specificity and cost effectiveness, primers of 16-24 nucleotides in length may be preferred. Those skilled in the art are well versed in the design of primers for use processes such as PCR. If required, probing can be done with entire restriction fragments of the gene disclosed herein which may be 100&#39;s or even 1000&#39;s of nucleotides in length. 
     “Coding sequence” refers to a DNA or RNA sequence that codes for a specific amino acid sequence and excludes the non-coding sequences. It may constitute an “uninterrupted coding sequence”, i.e., lacking an intron, such as in a cDNA or it may include one or more introns bounded by appropriate splice junctions. An “intron” is a sequence of RNA which is contained in the primary transcript but which is removed through cleavage and re-ligation of the RNA within the cell to create the mature mRNA that can be translated into a protein. 
     The terms “open reading frame” and “ORF” refer to the amino acid sequence encoded between translation initiation and termination codons of a coding sequence. The terms “initiation codon” and “termination codon” refer to a unit of three adjacent nucleotides (‘codon’) in a coding sequence that specifies initiation and chain termination, respectively, of protein synthesis (mRNA translation). 
     A “functional RNA” refers to an antisense RNA, ribozyme, or other RNA that is not translated. 
     The term “RNA transcript” refers to the product resulting from RNA polymerase catalyzed transcription of a DNA sequence. When the RNA transcript is a perfect complementary copy of the DNA sequence, it is referred to as the primary transcript or it may be a RNA sequence derived from post-transcriptional processing of the primary transcript and is referred to as the mature RNA. “Messenger RNA” (mRNA) refers to the RNA that is without introns and that can be translated into protein by the cell. “cDNA” refers to a single- or a double-stranded DNA that is complementary to and derived from mRNA. 
     “Regulatory sequences” and “suitable regulatory sequences” each refer to nucleotide sequences located upstream (5′ non-coding sequences), within, or downstream (3′ non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences include enhancers, promoters, translation leader sequences, introns, and polyadenylation signal sequences. They include natural and synthetic sequences as well as sequences which may be a combination of synthetic and natural sequences. As is noted above, the term “suitable regulatory sequences” is not limited to promoters. 
     “5′ non-coding sequence” refers to a nucleotide sequence located 5′ (upstream) to the coding sequence. It is present in the fully processed mRNA upstream of the initiation codon and may affect processing of the primary transcript to mRNA, mRNA stability or translation efficiency. (Turner et al.,  Molecular Biotechnology,  3:225 (1995)). 
     “3′ non-coding sequence” refers to nucleotide sequences located 3′ (downstream) to a coding sequence and include polyadenylation signal sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3′ end of the mRNA precursor. 
     The term “translation leader sequence” refers to that DNA sequence portion of a gene between the promoter and coding sequence that is transcribed into RNA and is present in the fully processed mRNA upstream (5′ ) of the translation start codon. The translation leader sequence may affect processing of the primary transcript to mRNA, mRNA stability or translation efficiency. 
     The term “mature” protein refers to a post-translationally processed polypeptide without its signal peptide. “Precursor” protein refers to the primary product of translation of an mRNA. “Signal peptide” refers to the amino terminal extension of a polypeptide, which is translated in conjunction with the polypeptide forming a precursor peptide and which is required for its entrance into the secretory pathway. The term “signal sequence” refers to a nucleotide sequence that encodes the signal peptide. 
     The term “intracellular localization sequence” refers to a nucleotide sequence that encodes an intracellular targeting signal. An “intracellular targeting signal” is an amino acid sequence that is translated in conjunction with a protein and directs it to a particular sub-cellular compartment. “Endoplasmic reticulum (ER) stop transit signal” refers to a carboxy-terminal extension of a polypeptide, which is translated in conjunction with the polypeptide and causes a protein that enters the secretory pathway to be retained in the ER. “ER stop transit sequence” refers to a nucleotide sequence that encodes the ER targeting signal. 
     “Promoter” refers to a nucleotide sequence, usually upstream (5′) to its coding sequence, that controls the expression of the coding sequence by providing the recognition for RNA polymerase and other factors required for proper transcription. “Promoter” includes a minimal promoter that is a short DNA sequence comprised of a TATA-box and other sequences that serve to specify the site of transcription initiation, to which regulatory elements are added for control of expression. “Promoter” also refers to a nucleotide sequence that includes a minimal promoter plus regulatory elements that is capable of controlling the expression of a coding sequence or functional RNA. This type of promoter sequence consists of proximal and more distal upstream elements, the latter elements often referred to as enhancers. Accordingly, an “enhancer” is a DNA sequence which can stimulate promoter activity and may be an innate element of the promoter or a heterologous element inserted to enhance the level or tissue specificity of a promoter. It is capable of operating in both orientations (normal or flipped), and is capable of functioning even when moved either upstream or downstream from the promoter. Both enhancers and other upstream promoter elements bind sequence-specific DNA-binding proteins that mediate their effects. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even be comprised of synthetic DNA segments. A promoter may also contain DNA sequences that are involved in the binding of protein factors which control the effectiveness of transcription initiation in response to physiological or developmental conditions. 
     The “initiation site” is the position surrounding the first nucleotide that is part of the transcribed sequence, which is also defined as position +1. With respect to this site all other sequences of the gene and its controlling regions are numbered. Downstream sequences (i.e. further protein encoding sequences in the 3′ direction) are denominated positive, while upstream sequences (mostly of the controlling regions in the 5′ direction) are denominated negative. 
     Promoter elements, particularly a TATA element, that are inactive or that have greatly reduced promoter activity in the absence of upstream activation are referred to as “minimal or core promoters.” In the presence of a suitable transcription factor, the minimal promoter functions to permit transcription. A “minimal or core promoter” thus consists only of all basal elements needed for transcription initiation, e.g., a TATA box and/or an initiator. 
     “Inducible promoter” refers to those regulated promoters that can be turned on in one or more cell types by an external stimulus, such as a chemical, light, hormone, stress, or a pathogen. 
     “Operably-linked” refers to the association of nucleic acid sequences on single nucleic acid fragment so that the function of one is affected by the other. For example, a regulatory DNA sequence is said to be “operably linked to” or “associated with” a DNA sequence that codes for an RNA or a polypeptide if the two sequences are situated such that the regulatory DNA sequence affects expression of the coding DNA sequence (i.e., that the coding sequence or functional RNA is under the transcriptional control of the promoter). Coding sequences can be operably-linked to regulatory sequences in sense or antisense orientation. 
     “Expression” refers to the transcription and/or translation of an endogenous gene or a transgene in cells. For example, in the case of antisense constructs, expression may refer to the transcription of the antisense DNA only. In addition, expression refers to the transcription and stable accumulation of sense (mRNA) or functional RNA. Expression may also refer to the production of protein. 
     The analysis of transcription start points in practically all promoters has revealed that there is usually no single base at which transcription starts, but rather a more or less clustered set of initiation sites, each of which accounts for some start points of the mRNA. Since this distribution varies from promoter to promoter the sequences of the reporter mRNA in each of the populations would differ from each other. Since each mRNA species is more or less prone to degradation, no single degradation rate can be expected for different reporter mRNAs. It has been shown for various eukaryotic promoter sequences that the sequence surrounding the initiation site (‘initiator’) plays an important role in determining the level of RNA expression directed by that specific promoter. This includes also part of the transcribed sequences. The direct fusion of promoter to reporter sequences would therefore lead to much suboptimal levels of transcription. 
     A commonly used procedure to analyze expression patterns and levels is through determination of the ‘steady state’ level of protein accumulation in a cell. Commonly used candidates for the reporter gene, known to those skilled in the art are 9-glucuronidase (GUS), growth hormone (GH), Chloramphenicol Acetyl Transferase (CAT) and proteins with fluorescent properties, such as Green Fluorescent Protein (GFP) from  Aequora victoria . In principle, however, many more proteins are suitable for this purpose, provided the protein does not interfere with essential cell functions. For quantification and determination of localization a number of tools are suited. Detection systems can readily be created or are available which are based on e.g. immunochemical, enzymatic, fluorescent detection and quantification. Protein levels can be determined in cell extracts or in intact tissue using in situ analysis of protein expression. 
     Generally, individual transformed lines with one chimeric promoter reporter construct will vary in their levels of expression of the reporter gene. Also frequently observed is the phenomenon that such transformants do not express any detectable product (RNA or protein). The variability in expression is commonly ascribed to position effects, although the molecular mechanisms underlying this inactivity are usually not clear. 
     “Non-specific expression” refers to constitutive expression or low level, basal (‘leaky’) expression in nondesired cells or tissues from a ‘regulated promoter’. 
     “Antisense inhibition” refers to the production of antisense RNA transcripts capable of suppressing the expression of protein from an endogenous gene or a transgene. 
     “Co-suppression” and “transwitch” each refer to the production of sense RNA transcripts capable of suppressing the expression of identical or substantially similar transgene or endogenous genes (U.S. Pat. No. 5,231,020). 
     “Homologous to” refers to the similarity between the nucleotide sequence of two nucleic acid molecules or between the amino acid sequences of two protein molecules. Estimates of such homology are provided by either DNA-DNA or DNA-RNA hybridization under conditions of stringency as is well understood by those skilled in the art (as described in Haines and Higgins (eds.), Nucleic Acid Hybridization, IRL Press, Oxford, U.K.), or by the comparison of sequence similarity between two nucleic acids or proteins. 
     The term “substantially similar” refers to nucleotide and amino acid sequences that represent equivalents of the instant inventive sequences. For example, altered nucleotide sequences which simply reflect the degeneracy of the genetic code but nonetheless encode amino acid sequences that are identical to the inventive amino acid sequences are substantially similar to the inventive sequences. In addition, amino acid sequences that are substantially similar to the instant sequences are those wherein overall amino acid identity is 95% or greater to the instant sequences. Modifications to the instant invention that result in equivalent nucleotide or amino acid sequences is well within the routine skill in the art. Moreover, the skilled artisan recognizes that equivalent nucleotide sequences encompassed by this invention can also be defined by their ability to hybridize, under stringent conditions (0.1×SSC, 0.1% SDS, 65° C.), with the nucleotide sequences that are within the literal scope of the instant claims. 
     “Transgene activation system” refers to the expression system comprised of an inactive transgene and a chimeric site-specific recombinase gene, functioning together, to effect transgene expression in a regulated manner. The specificity of the recombination will be determined by the specificity of regulated promoters as well as the use of wild-type or mutant site-specific sequences. Both elements of the system can be chromosomally integrated and inherited independently. 
     “Target gene” refers to a gene on the replicon that expresses the desired target coding sequence, Functional RNA, or protein. The target gene is not essential for replicon replication. Additionally, target genes may comprise native non-viral genes inserted into a non-native organism, or chimeric genes, and will be under the control of suitable regulatory sequences. Thus, the regulatory sequences in the target gene may come from any source, including the virus. 
     “Transcription Stop Fragment” refers to nucleotide sequences that contain one or more regulatory signals, such as polyadenylation signal sequences, capable of terminating transcription. Examples include the 3′ non-regulatory regions of genes encoding nopaline synthase and the small subunit of ribulose bisphosphate carboxylase. 
     “Translation Stop Fragment” refers to nucleotide sequences that contain one or more regulatory signals, such as one or more termination codons in all three frames, capable of terminating translation. Insertion of a translation stop fragment adjacent to or near the initiation codon at the 5′ end of the coding sequence will result in no translation or improper translation. Excision of the translation stop fragment by site-specific recombination will leave a site-specific sequence in the coding sequence that does not interfere with proper translation using the initiation codon. 
     “Blocking fragment” refers to a DNA fragment that is flanked by site specific sequences that can block the transcription and/or the proper translation of a coding sequence resulting in an inactive transgene. When the blocking fragment contains polyadenylation signal sequences and other sequences encoding regulatory signals capable of terminating transcription, it can block the transcription of a coding sequence when placed in the 5′ non-translated region, i.e., between the transcription start site and the ORF. When inserted in the coding sequence a blocking fragment can block proper translation by disrupting its open reading frame. DNA rearrangement by site-specific recombination can restore transcription and/or proper translatability. For example, excision of the blocking fragment by site-specific recombination leaves behind a site-specific sequence that allows transcription and/or proper translatability. A Transcription or Translational Stop Fragment will be considered a blocking fragment. 
     The terms “in cis” and “in trans” refer to the presence of DNA elements, such as the viral origin of replication and the replication protein(s) gene, on the same DNA molecule or on a different DNA molecule, respectively. 
     The terms “cis-acting sequence” and “cis-acting element” refer to DNA or RNA sequences whose functions require them to be on the same molecule. An example of a cis-acting sequence on the replicon is the viral replication origin. 
     The terms “trans-acting sequence” and “trans-acting element” refer to DNA or RNA sequences whose function does not require them to be on the same molecule. 
     “Cis-acting viral sequences” refers to viral sequences necessary for viral replication (such as the replication origin) and in cis orientation. 
     “Transactivating gene” refers to a gene encoding a transactivating protein. It can encode a viral replication protein(s) or a site-specific replicase. It can be a natural gene, for example, a viral replication gene, or a chimeric gene, for example, when regulatory sequences are operably-linked to the open reading frame of a site-specific recombinase or a viral replication protein. “Transactivating genes” may be chromosomally integrated or transiently expressed. 
     “Wild-type” refers to the normal gene, virus, or organism found in nature without any known mutation. 
     “Genome” refers to the complete genetic material of an organism. The term “nucleic acid” refers to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form, composed of monomers (nucleotides) containing a sugar, phosphate and a base which is either a purine or pyrimidine. Unless specifically limited, the term encompasses nucleic acids containing known analogs of natural nucleotides which have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions) and complementary sequences as well as the sequence explicitly indicated. Specifically, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al.,  Nucleic Acid Res.,  19, 5081 (1991); Ohtsuka et al.,  J. Biol. Chem.,  260, 2605 (1985); Rossolini et al.,  Mol. Cell. Probes,  8, 91 (1994)). A “nucleic acid fragment” is a fraction of a given nucleic acid molecule. In higher animals, deoxyribonucleic acid (DNA) is the genetic material while ribonucleic acid (RNA) is involved in the transfer of information contained within DNA into proteins. A “genome” is the entire body of genetic material contained in each cell of an organism. The term “nucleotide sequence” refers to a polymer of DNA or RNA which can be single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases capable of incorporation into DNA or RNA polymers. The terms “nucleic acid” or “nucleic acid sequence” may also be used interchangeably with gene, cDNA, DNA and RNA encoded by a gene. 
     The invention encompasses isolated or substantially purified nucleic acid or protein compositions. In the context of the present invention, an “isolated” or “purified” DNA molecule or an “isolated” or “purified” polypeptide is a DNA molecule or polypeptide that, by the hand of man, exists apart from its native environment and is therefore not a product of nature. An isolated DNA molecule or polypeptide may exist in a purified form or may exist in a non-native environment such as, for example, a transgenic host cell. For example, an “isolated” or “purified” nucleic acid molecule or protein, or biologically active portion thereof, is substantially free of other cellular material, or culture medium when produced by recombinant techniques, or substantially free of chemical precursors or other chemicals when chemically synthesized. Preferably, an “isolated” nucleic acid is free of sequences (preferably protein encoding sequences) that naturally flank the nucleic acid (i.e., sequences located at the 5′ and 3′ ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated nucleic acid molecule can contain less than about 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb, or 0.1 kb of nucleotide sequences that naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived. A protein that is substantially free of cellular material includes preparations of protein or polypeptide having less than about 30%, 20%, 10%, 5%, (by dry weight) of contaminating protein. When the protein of the invention, or biologically active portion thereof, is recombinantly produced, preferably culture medium represents less than about 30%, 20%, 10%, or 5% (by dry weight) of chemical precursors or non-protein-of-interest chemicals. Fragments and variants of the disclosed nucleotide sequences and proteins or partial-length proteins encoded thereby are also encompassed by the present invention. By “fragment” is intended a portion of the nucleotide sequence or a portion of the amino acid sequence, and hence a portion of the polypeptide or protein, encoded thereby. Alternatively, fragments of a nucleotide sequence that are useful as hybridization probes generally do not encode fragment proteins retaining biological activity. Thus, fragments of a nucleotide sequence may range from at least about 9 nucleotides, about 12 nucleotides, about 20 nucleotides, about 50 nucleotides, about 100 nucleotides or more. 
     By “variants” is intended substantially similar sequences. For nucleotide sequences, variants include those sequences that, because of the degeneracy of the genetic code, encode the identical amino acid sequence of the native protein. Naturally occurring allelic variants such as these can be identified with the use of well-known molecular biology techniques, as, for example, with polymerase chain reaction (PCR) and hybridization techniques. Variant nucleotide sequences also include synthetically derived nucleotide sequences, such as those generated, for example, by using site-directed mutagenesis which encode the native protein, as well as those that encode a polypeptide having amino acid substitutions. Generally, nucleotide sequence variants of the invention will have at least 40, 50, 60, to 70%, e.g., preferably 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, to 79%, generally at least 80%, e.g., 81%-84%, at least 85%, e.g., 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, to 98% sequence identity to the native nucleotide sequence. 
     By “variant” polypeptide is intended a polypeptide derived from the native protein by deletion (so-called truncation) or addition of one or more amino acids to the N-terminal and/or C-terminal end of the native protein; deletion or addition of one or more amino acids at one or more sites in the native protein; or substitution of one or more amino acids at one or more sites in the native protein. Such variants may results form, for example, genetic polymorphism or from human manipulation. Methods for such manipulations are generally known in the art. 
     Thus, the polypeptides of the invention may be altered in various ways including amino acid substitutions, deletions, truncations, and insertions. Methods for such manipulations are generally known in the art. For example, amino acid sequence variants of the polypeptides can be prepared by mutations in the DNA. Methods for mutagenesis and nucleotide sequence alterations are well known in the art. See, for example, Kunkel,  Proc. Natl. Acad. Sci. USA,  82, 488 (1985); Kunkel et al.,  Methods in Enzymol.,  154, 367 (1987); U.S. Pat. No.4,873,192; Walker and Gaastra, eds.,  Techniques in Molecular Biology , MacMillan Publishing Company, New York (1983) and the references cited therein. Guidance as to appropriate amino acid substitutions that do not affect biological activity of the protein of interest may be found in the model of Dayhoff et al.,  Atlas of Protein Sequence and Structure , Natl. Biomed. Res. Found., Washington, C.D. (1978), herein incorporated by reference. Conservative substitutions, such as exchanging one amino acid with another having similar properties, are preferred. 
     Thus, the genes and nucleotide sequences of the invention include both the naturally occurring sequences as well as mutant forms. Likewise, the polypeptides of the invention encompass both naturally occurring proteins as well as variations and modified forms thereof. Such variants will continue to possess the desired activity. The deletions, insertions, and substitutions of the polypeptide sequence encompassed herein are not expected to produce radical changes in the characteristics of the polypeptide. However, when it is difficult to predict the exact effect of the substitution, deletion, or insertion in advance of doing so, one skilled in the art will appreciate that the effect will be evaluated by routine screening assays. 
     “Expression cassette” as used herein means a DNA sequence capable of directing expression of a particular nucleotide sequence in an appropriate host cell, comprising a promoter operably linked to the nucleotide sequence of interest which is operably linked to termination signals. It also typically comprises sequences required for proper translation of the nucleotide sequence. The coding region usually codes for a protein of interest but may also code for a functional RNA of interest, for example antisense RNA or a nontranslated RNA, in the sense or antisense direction. The expression cassette comprising the nucleotide sequence of interest may be chimeric, meaning that at least one of its components is heterologous with respect to at least one of its other components. The expression cassette may also be one which is naturally occurring but has been obtained in a recombinant form useful for heterologous expression. The expression of the nucleotide sequence in the expression cassette may be under the control of a constitutive promoter or of an inducible promoter which initiates transcription only when the host cell is exposed to some particular external stimulus. In the case of a multicellular organism, the promoter can also be specific to a particular tissue or organ or stage of development. 
     The proteins of the invention may be altered in various ways including amino acid substitutions, deletions, truncations, and insertions. Methods for such manipulations are generally known in the art. Methods for mutagenesis and nucleotide sequence alterations are well known in the art. See, for example, Kunkel,  Proc. Natl. Acad. Sci. USA,  82:488-492 (1985); Kunkel et al.,  Methods in Enzymol.  154:367-382 (1987); U.S. Pat. No. 4,873,192; Walker and Gaastra, eds. (1983)  Techniques in Molecular Biology  (MacMillan Publishing Company, New York) and the references cited therein. Guidance as to appropriate amino acid substitutions that do not affect biological activity of the protein of interest may be found in the model of Dayhoffet al. (1978)  Atlas of Protein Sequence and Structure  (Natl. Biomed. Res. Found., Washington, D.C.), herein incorporated by reference. Conservative substitutions, such as exchanging one amino acid with another having similar properties, may be preferred. 
     Thus, the genes and nucleotide sequences of the invention include both the naturally occurring sequences as well as mutant forms. Likewise, the proteins of the invention encompass both naturally occurring proteins as well as variations and modified forms thereof. Such variants will continue to possess the desired disease resistance activity. Obviously, the mutations that will be made in the DNA encoding the variant must not place the sequence out of reading frame and preferably will not create complementary regions that could produce secondary mRNA structure. See, EP Patent Application Publication No. 75,444. 
     The deletions, insertions, and substitutions of the protein sequence encompassed herein are not expected to produce radical changes in the characteristics of the protein. However, when it is difficult to predict the exact effect of the substitution, deletion, or insertion in advance of doing so, one skilled in the art will appreciate that the effect will be evaluated by routine screening assays. Hybridization of such sequences may be carried out under stringent conditions. 
     “Stringent hybridization conditions” and “stringent hybridization wash conditions” in the context of nucleic acid hybridization experiments such as Southern and Northern hybridization are sequence dependent, and are different under different environmental parameters. Longer sequences hybridize specifically at higher temperatures. An extensive guide to the hybridization of nucleic acids is found in Tijssen,  Laboratory Techniques in Biochemistry and Molecular biology—Hybridization with Nucleic Acid Probes , page 1, chapter 2 “Overview of principles of hybridization and the strategy of nucleic acid probe assays” Elsevier, New York (1993). Generally, highly stringent hybridization and wash conditions are selected to be about 5° C. lower than the thermal melting point (T m ) for the specific sequence at a defined ionic strength and pH. Typically, under “stringent conditions” a probe will hybridize to its target subsequence, but to no other sequences. For example, by “stringent conditions” or “stringent hybridization conditions” is intended conditions under which a probe will hybridize to its target sequence to a detectably greater degree than to other sequences (e.g., at least 2- fold over background). By controlling the stringency of the hybridization and/or washing conditions, target sequences that are 100% complementary to the probe can be identified (homologous probing). Alternatively, stringency conditions can be adjusted to allow some mismatching in sequences so that lower degrees of similarity are detected (heterologous probing). Generally, a probe is less than about 1000 nucleotides in length, preferably less than 500 nucleotides in length. 
     Typically, stringent conditions will be those in which the salt concentration is less than about 1.5 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C. for short probes (e.g., 10 to 50 nucleotides) and at least about 60° C. for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide. 
     Exemplary low stringency conditions include hybridization with a buffer solution of 30 to 35% formamide, 1 M NaCl, 1% SDS (sodium dodecyl sulphate) at 37° C., and a wash in 1× to 2×SSC (20×SSC=3.0 M NaCl/0.3 M trisodium citrate) at 50 to 55° C. Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1.0 M NaCl, 1% SDS at 37° C., and a wash in 0.5× to 1×SSC at 55 to 60° C. Exemplary high stringency conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60 to 65° C. 
     Specificity is typically the function of post-hybridization washes, the critical factors being the ionic strength and temperature of the final wash solution. For DNA-DNA hybrids, the T m  can be approximated from the equation of Meinkoth and Wahl  Anal. Biochem.  138:267-284 (1984); T m 81.5°C.+16.6 (log M) +0.41 (%GC) −0.61 (% form) −500/L; where M is the molarity of monovalent cations, %GC is the percentage of guanosine and cytosine nucleotides in the DNA, % form is the percentage of formamide in the hybridization solution, and L is the length of the hybrid in base pairs. The T m  is the temperature (under defined ionic strength and pH) at which 50% of a complementary target sequence hybridizes to a perfectly matched probe. 
     Very stringent conditions are selected to be equal to the T m  for a particular probe. An example of stringent hybridization conditions for hybridization of complementary nucleic acids which have more than 100 complementary residues on a filter in a Southern or Northern blot is 50% formamide with 1 mg of heparin at 42° C., with the hybridization being carried out overnight. An example of highly stringent conditions is 0.15 M NaCl at 72° C. for about 15 minutes. An example of stringent wash conditions is a 0.2×SSC wash at 65° C. for 15 minutes (see, Sambrook, infra, for a description of SSC buffer). Often, a high stringency wash is preceded by a low stringency wash to remove background probe signal. An example of medium stringency for a duplex of, e.g., more than 100 nucleotides, is 1×SSC at 45° C. for 15 minutes. An example low stringency wash for a duplex of, e.g., more than 100 nucleotides, is 4-6×SSC at 40° C. for 15 minutes. For short probes (e.g., about 10 to 50 nucleotides), stringent conditions typically involve salt concentrations of less than about 1.0 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other slats) at pH 7.0 to 8.3, and the temperature is typically at least about 30° C. Stringent conditions can also be achieved with the addition of destabilizing agents such as formamide. In general, a signal to noise ratio of 2× (or higher) than that observed for an unrelated probe in the particular hybridization assay indicates detection of a specific hybridization. Nucleic acids that do not hybridize to each other under stringent conditions are still substantially identical if the proteins that they encode are substantially identical. This occurs, e.g., when a copy of a nucleic acid is created using the maximum codon degeneracy permitted by the genetic code. 
     The following are examples of sets of hybridization/wash conditions that may be used to clone homologous nucleotide sequences that are substantially identical to reference nucleotide sequences of the present invention: a reference nucleotide sequence preferably hybridizes to the reference nucleotide sequence in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 2×SSC, 0.1% SDS at 50° C., more desirably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 1×SSC, 0.1% SDS at 50° C., more desirably still in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 0.5×SSC, 0.1% SDS at 50° C., preferably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 50° C., more preferably in 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO 4 , 1 mM EDTA at 50° C. with washing in 0.1×SSC, 0.1% SDS at 65° C. 
     T m  is reduced by about 1° C. for each 1% of mismatching; thus, T m , hybridization, and/or wash conditions can be adjusted to hybridize to sequences of the desired identity. For example, if sequences with &gt;90% identity are sought, the T m  can be decreased 10° C. Generally, stringent conditions are selected to be about 5° C. lower than the thermal melting point (T m ) for the specific sequence and its complement at a defied ionic strength and pH. However, severely stringent conditions can utilize a hybridization and/or wash at 1, 2, 3, or 4° C. lower than the thermal melting point (T m ); moderately stringent conditions can utilize a hybridization and/or wash at 6, 7, 8, 9, or 10° C. lower than the thermal melting point (T m ); low stringency conditions can utilize a hybridization and/or wash at 11, 12, 13, 14, 15, or 20° C. lower than the thermal melting point (T m ). Using these parameters, hybridization and wash compositions, and desired T, those of ordinary skill will understand that variations in the stringency of hybridization and/or wash solutions are inherently described. If the desired degree of mismatching results in a T of less than 45° C. (aqueous solution) or 32° C. (formamide solution), it is preferred to increase the SSC concentration so that a higher temperature can be used. An extensive guide to the hybridization of nucleic acids is found in Tijssen (1993) Laboratory Techniques in Biochemistry and Molecular Biology-Hybridization with Nucleic Acid Probes, Part 1, Chapter 2 (Elsevier, New York); and Ausubel et al., eds. (1995) Current Protocols in Molecular Biology, Chapter 2 (Greene Publishing and Wiley—Interscience, New York). See Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual (2d ed., Cold Spring Harbor Laboratory Press, Plainview, N.Y.). 
     Generally, stringent conditions are selected to be about 5° C. lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. However, stringent conditions encompass temperatures in the range of about 1° C. to about 20° C., depending upon the desired degree of stringency as otherwise qualified herein. Nucleic acids that do not hybridize to each other under stringent conditions are still substantially identical if the polypeptides they encode are substantially identical. This may occur, e.g., when a copy of a nucleic acid is created using the maximum codon degeneracy permitted by the genetic code. One indication that two nucleic acid sequences are substantially identical is when the polypeptide encoded by the first nucleic acid is immunologically cross reactive with the polypeptide encoded by the second nucleic acid. 
     “Vector” is defined to include, inter alia, any plasmid, cosmid, or phage in double or single stranded linear or circular form which may or may not be self transmissible or mobilizable, and which can transform prokaryotic or eukaryotic host either by integration into the cellular genome or exist extrachromosomally (e.g. autonomous replicating plasmid with an origin of replication). 
     Specifically included are shuttle vectors by which is meant a DNA vehicle capable, naturally or by design, of replication in two different host organisms, which may be selected from actinomycetes and related species, bacteria and eucaryotic (e.g. higher cell, mammalian, yeast or fungal cells). 
     Preferably the nucleic acid in the vector is under the control of, and operably linked to, an appropriate promoter or other regulatory elements for transcription in a host cell such as a microbial, e.g. bacterial, or animal cell. The vector may be a bi-functional expression vector which functions in multiple hosts. In the case of genomic DNA, this may contain its own promoter or other regulatory elements and in the case of cDNA this may be under the control of an appropriate promoter or other regulatory elements for expression in the host cell. 
     “Cloning vectors” typically contain one or a small number of restriction endonuclease recognition sites at which foreign DNA sequences can be inserted in a determinable fashion without loss of essential biological function of the vector, as well as a marker gene that is suitable for use in the identification and selection of cells transformed with the cloning vector. Marker genes typically include genes that provide tetracycline resistance, hygromycin resistance or ampicillin resistance. 
     “Operably linked” means joined as part of the same nucleic acid molecule, suitably positioned and oriented for transcription to be initiated from the promoter. DNA operably linked to a promoter is “under transcriptional initiation regulation” of the promoter. 
     “Chimeric” is used to indicate that a DNA sequence, such as a vector or a gene, is comprised of more than one DNA sequences of distinct origin with are fused together by recombinant DNA techniques resulting in a DNA sequence, which does not occur naturally. 
     The terms “heterologous DNA sequence,” “exogenous DNA segment” or “heterologous nucleic acid,” as used herein, each refer to a sequence that originates from a source foreign to the particular host cell or, if from the same source, is modified from its original form. Thus, a heterologous gene in a host cell includes a gene that is endogenous to the particular host cell but has been modified through, for example, the use of DNA shuffling. The terms also include non-naturally occurring multiple copies of a naturally occurring DNA sequence. Thus, the terms refer to a DNA segment that is foreign or heterologous to the cell, or homologous to the cell but in a position within the host cell nucleic acid in which the element is not ordinarily found. Exogenous DNA segments are expressed to yield exogenous polypeptides. 
     A “homologous” DNA sequence is a DNA sequence that is naturally associated with a host cell into which it is introduced. 
     The following terms are used to describe the sequence relationships between two or more nucleic acids or polynucleotides: (a) “reference sequence”, (b) “comparison window”, (c) “sequence identity”, (d) “percentage of sequence identity”, and (e) “substantial identity”. 
     As used herein, “reference sequence” is a defined sequence used as a basis for sequence comparison. A reference sequence may be a subset or the entirety of a specified sequence; for example, as a segment of a full length cDNA or gene sequence, or the complete cDNA or gene sequence. 
     As used herein, “comparison window” makes reference to a contiguous and specified segment of a polynucleotide sequence, wherein the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. Generally, the comparison window is at least 20 contiguous nucleotides in length, and optionally can be 30, 40, 50, 100, or longer. Those of skill in the art understand that to avoid a high similarity to a reference sequence due to inclusion of gaps in the polynucleotide sequence a gap penalty is typically introduced and is subtracted from the number of matches. 
     Methods of alignment of sequences for comparison are well known in the art. Thus, the determination of percent identity between any two sequences can be accomplished using a mathematical algorithm. Preferred, non-limiting examples of such mathematical algorithms are the algorithm of Myers and Miller,  CABIOS  4:11-17 (1988); the local homology algorithm of Smith et al.  Adv. Appl. Math.  2:482 (1981); the homology alignment algorithm of Needleman and Wunsch  J. Mol. Biol.  48:443-453 (1970); the search-for-similarity-method of Pearson and Lipman  Proc. Natl. Acad. Sci.  85:2444-2448 (1988); the algorithm of Karlin and Altschul,  Proc. Nath. Acad Sci. USA  872264 (1990), modified as in Karlin and Altschul,  Proc. Nath. Acad. Sci. USA  90:5873-5877 (1993). 
     Computer implementations of these mathematical algorithms can be utilized for comparison of sequences to determine sequence identity. Such implementations include, but are not limited to: CLUSTAL in the PC/Gene program (available from Intelligenetics, Mountain View, Calif.); the ALIGN program (Version 2.0) and GAP, BESTFIT, BLAST, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Version 8 (available from Genetics Computer Group (GCG), 575 Science Drive, Madison, Wis., USA). Alignments using these programs can be performed using the default parameters. The CLUSTAL program is well described by Higgins et al.  Gene  73:237 244 (1988); Higgins et al.  CABIOS  5:151-153 (1989); Corpet et al.  Nucleic Acids Res.  16:10881-90 (1988); Huang et al.  CABIOS  8:155-65 (1992); and Pearson et al.  Meth. Mol. Biol.  24:307-331 (1994). The ALIGN program is based on the algorithm of Myers and Miller, supra. The BLAST programs of Altschul et al.,  J. Mol. Biol.  215:403 (1990), are based on the algorithm of Karlin and Altschul supra. To obtain gapped alignments for comparison purposes, Gapped BLAST (in BLAST 2.0) can be utilized as described in Altschul et al.  Nucleic Acids Res.  25:3389 (1997). Alternatively, PSI-BLAST (in BLAST 2.0) can be used to perform an iterated search that detects distant relationships between molecules. See Altschul et al., supra. When utilizing BLAST, Gapped BLAST, PSI-BLAST, the default parameters of the respective programs (e.g. BLASTN for nucleotide sequences, BLASTX for proteins) can be used. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, a cutoff of 100, M=5, N=−4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff &amp; Henikoff,  Proc. Natl. Acad. Sci. USA.  89, 10915 (1989)). See http:/www.ncbi.n1m.nih.gov. Alignment may also be performed manually by inspection. 
     For purposes of the present invention, comparison of nucleotide sequences for determination of percent sequence identity disclosed herein is preferably made using the BlastN program (version 1.4.7 or later) with its default parameters or any equivalent program. By “equivalent program” is intended any sequence comparison program that, for any two sequences in question, generates an alignment having identical nucleotide or amino acid residue matches and an identical percent sequence identity when compared to the corresponding alignment generated by the preferred program. 
     As used herein, “sequence identity” or “identity” in the context of two nucleic acid or polypeptide sequences makes reference to the residues in the two sequences that are the same when aligned for maximum correspondence over a specified comparison window. When percentage of sequence identity is used in reference to proteins it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity) and therefore do not change the functional properties of the molecule. When sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences that differ by such conservative substitutions are said to have “sequence similarity” or “similarity.” Means for making this adjustment are well known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, e.g., as implemented in the program PC/GENE (Intelligenetics, Mountain View, Calif.). 
     As used herein, “percentage of sequence identity” means the value determined by comparing two optimally aligned sequences over a comparison window, wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison, and multiplying the result by 100 to yield the percentage of sequence identity. 
     The term “substantial identity” of polynucleotide sequences means that a polynucleotide comprises a sequence that has at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, or 79%, preferably at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, or 89%, more preferably at least 90%, 91%, 92%, 93%, or 94%, and most preferably at least 95%, 96%, 97%, 98%, or 99% sequence identity, compared to a reference sequence using one of the alignment programs described using standard parameters. One of skill in the art will recognize that these values can be appropriately adjusted to determine corresponding identity of proteins encoded by two nucleotide sequences by taking into account codon degeneracy, amino acid similarity, reading frame positioning, and the like. Substantial identity of amino acid sequences for these purposes normally means sequence identity of at least 70%, more preferably at least 80%, 90%, and most preferably at least 95%. 
     Another indication that nucleotide sequences are substantially identical is if two molecules hybridize to each other under stringent conditions. Generally, stringent conditions are selected to be about 5° C. lower than the thermal melting point (T m ) for the specific sequence at a defined ionic strength and pH. However, stringent conditions encompass temperatures in the range of about 1° C. to about 20° C., depending upon the desired degree of stringency as otherwise qualified herein. Nucleic acids that do not hybridize to each other under stringent conditions are still substantially identical if the polypeptides they encode are substantially identical. This may occur, e.g., when a copy of a nucleic acid is created using the maximum codon degeneracy permitted by the genetic code. One indication that two nucleic acid sequences are substantially identical is when the polypeptide encoded by the first nucleic acid is immunologically cross reactive with the polypeptide encoded by the second nucleic acid. 
     The term “substantial identity” in the context of a peptide indicates that a peptide comprises a sequence with at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, or 79%, preferably 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, or 89%, more preferably at least 90%, 91%, 92%, 93%, or 94%, or even more preferably, 95%, 96%, 97%, 98% or 99%, sequence identity to the reference sequence over a specified comparison window. Preferably, optimal alignment is conducted using the homology alignment algorithm of Needleman and Wunsch  J. Mol. Biol.  48:443-453 (1970). An indication that two peptide sequences are substantially identical is that one peptide is immunologically reactive with antibodies raised against the second peptide. Thus, a peptide is substantially identical to a second peptide, for example, where the two peptides differ only by a conservative substitution. 
     DETAILED DESCRIPTION OF THE INVENTION 
     This invention relates to peptide amino acid absorption in the dog, and more particularly, to separate, whole or partial-length, complementary DNA encoding putative canine low-affinity, high-capacity H + /peptide transport proteins (cPepT1), mRNA transcripts corresponding to cPepT1, characterization of cPepT1 by glycylsarcosine (GlySar) uptake, identification of dipeptides, tripeptides, and tetrapeptides well recognized by cPepT1, and the effect of supplemental peptide substrate on the transport capacity of cPepT1. 
     The invention also provides a pet food composition comprising at least one dipeptide, tripeptide, or tetrapeptide that provides enhanced uptake of amino acids by PepT1. A typical canine diet for use in the present invention may also, for example, contain about 20 to about 30% crude protein, about 10 to about 20% fat, and about 10% total dietary fiber. However, no specific ratios or percentages of these or other nutrients are required. 
     The inventors have discovered a method for identifying peptides (e.g. dipeptides, tripeptides, or tetrapeptides) that increase transport of amino acids by PepT1 using MDCK cells, particularly when incubated with lactalbumin hydrolysate and assayed at optimum time post-seeding, as indicated in Example 2. 
     In order that the invention may be more readily understood, reference is made to the following examples which are intended to illustrate the invention, but not limit the scope thereof. 
     EXAMPLE 1 
     Generation of Partial-length Canine PepT1 cDNA 
     Partial Cloning of Canine PepT1 (cPepT1) from Small Intestinal Epithelium 
     Initial attempts (over 150) to partially clone the putative canine PepT1 cDNA by reverse transcriptase-polymerase chain reaction (RT-PCR) methodology failed. The source of mRNA was canine liver tissue that had been frozen for about 6 months (supplied by Dr. Randal Buddington, Mississippi State University) and oligomer primers were based on the rabbit PepT1 sequence. Subsequently, frozen canine “mid” small intestine (jejunal) tissue segments became available (supplied by Dr. Buddington) and a partial length cDNA of about 780 base pairs (bp) was cloned by RT-PCR. Total RNA was isolated from jejunal epithelium scraped from intestinal sections using a standard acidic phenol-chloroform protocol. One μg of mRNA was isolated from total RNA using POLY A TRACT SYSTEM® (Promega, Madison, Wis.) and reversed transcribed using murine leukemia virus reverse transcriptase (Perkin Elmer, Foster City, Calif.) and oligo(dT) primers (Gibco BRL, Grand Island, N.Y.). Successful PCR reactions were 50 μL and contained 1 μM MgCl 2  and Taq polymerase (Perkin Elmer). Twenty-five thermal cycles of 94° C. for 1 min, 40° C. for 45 sec, and 72° C. for 1 min were used. The cycles were preceded by a 55 sec denaturization of the RT product at 95° C., followed by a 10 min extension of RT-PCR products at 72° C. More than 150 RT-PCR reactions testing ten different primer sets were required to achieve this protocol. The resulting cDNA using Primer Set 4 (FIG. 1) was TA-cloned into the pCR®II vector (Invitrogen, Carlsbad, Calif.), plasmid-containing colonies selected by blue/white screening, and amplified following instructions of the manufacturer. Restriction analyses of recovered pCR®II/cDNA plasmids revealed that four of fifty-six clones contained cDNA consistent with rabbit PepT1 cDNA (FIG.  2 ). 
     Northern Blot Analysis of cPepT1 Expression in Dog Tissue and MDCK Cells 
     The potential expression of cPepT1 mRNA by canine kidney, small intestinal epithelium, and immortalized kidney distal tubule epithelial cells (Madin-Darby Canine Kidney, MDCK) was evaluated by Northern analyses using cDNA derived from canine jejunal epithelium (FIG.  3 ). RNA were subjected to 1% gel electrophoresis in the presence of 0.02 M formaldehyde, transferred by downward capillary action to 0.45-μm nylon membranes (Hybond-N, Amersham, Arlington Heights, Ill.), and covalently cross-linked by ultra-violet light. cDNA were randomly labeled with [ 32 P]-CTP using a kit (Gibco BRL), purified through Sephadex-50 columns (Amersham Pharmacia, Piscataway, N.J.), and hybridized with blots at 56° C. for 18 h. The blots were then washed 2 times at 56° C. for 15 min and once at 57° C. for 10 min. Autoradiographs were exposed to blots at 80° C. for 24 h and the size of the transcript determined by regression of hybridized bands against the migration distance of 18S (1.9 kb) and 28S (4.9 kb) RNA. 
     Each canine tissue-derived cDNA (TA-clone 26, FIG. 3A; TA-clone 6, FIG. 3B) hybridized to three mRNA species in dog kidney, dog small intestinal epithelium, and MDCK cells. To confirm identification of PepT1 mRNA by these canine cDNAs, RNA isolated from dog kidney and liver tissues were probed for expression of PepT1 mRNA using a full-length rabbit PepT1 cDNA (FIG. 4; rabbit PepT1 cDNA supplied by Drs. F. Leibach and V. Ganapathy, Medical College of Georgia). The results also demonstrated the expression of the same three PepT1 mRNA species by dog tissues, indicating that the full-length rabbit PepT1 cDNA and the cDNA derived from canine tissue in the present study identified the same transcripts. The mean/SD of transcript sizes calculated from these three blots were 4.2/0.22, 2.75/0.26, and 1.46/0.42 kb, respectively. Collectively, these data indicate that liver, intestinal epithelial, and MDCK cells express the same size and number of PepT1 transcripts. In comparison, various tissues of chicken, sheep, cow, pig, rabbit, rat, human, and Caco2 cells are reported to express a single transcript, with the principle difference in size being between chicken (1.9) and mammalian species (2.8, 2.8, 2.9, 2.9, 3.0, 3.1, 2.9, respectively 
     Partial Cloning and Sequence Identification of Canine PepT1 (cPepT1) cDNA from MDCK Cells 
     To confirm the positive Northern analysis, identification of PepT1 mRNA expression using cDNA generated from dog small intestinal epithelium, RT-PCR methodologies were used to generate a PepT1 cDNA from MDCK cells. The target cDNA region was a subset of the cDNA generated by RT-PCR from canine small intestine (bp 83 to 887 of rabbit PepT1). Accordingly, PCR primers that corresponded to bp 259 to 619 of rabbit PepT1 (GenBank acc. no. U06467) were used to generate a partial-length “canine PepT1” (cPepT1) cDNA from mRNA isolated from MDCK cells. RNA was collected from cells that were plated at 30,000 cm 2  on rat tail collagen-coated dishes and cultured for 3 days in 10% fetal calf serum/DMEM. Reverse transcription of 5 μg of total RNA by SUPERSCRIPT®II reverse transcriptase (Gibco-BRL) was performed using random and oligo(dT) primers, per instructions of the manufacturer (Gibco-BRL). All PCR reactions contained 2 mM MgCl 2  and thermal cycling using Taq polymerase included 30 cycles at 94° C. for 2 min, 55° C. for 1 min, and 72° C. for 2 min. The cycles were preceded by a 10 min denaturization of the RT product at 94° C., followed by a 10 min extension of RT-PCR products at 72° C. More than one hundred RT-PCR reactions were required to achieve this protocol. 
     The resulting cDNA of about 380 bp was TA-cloned, into the site of pCR®II vector (as described above), amplified, bacterial colonies evaluated by blue/white screening, and pCR®II/cDNA plasmids evaluated for cDNA by Eco RI/Pst I restriction analysis (as described above). Restriction analyses of recovered plasmids revealed that six of thirty-six clones contained cDNA consistent with rabbit PepT1 cDNA. Two of the confirmed plasmids were amplified in bacteria, recovered, and sent for sequencing by the University of Florida DNA Sequencing Core Facility (Gainesville). Sequence comparisons of this 380 bp cDNA (FIG. 5) to PepT1 sequences of other species using BLAST 2.0.14. software (blast@ncbi.nlm.nih.gov) revealed that the canine sequence shares sequence homology of 79% to rabbit (bp 259 to 640; GenBank acc. no. 473375), 83% to rat (bp 213 to 593; GenBank acc. no. D50664.1), 83% to mouse (bp 213 to 589; GenBank acc. no. AF205540), and 87% to human (bp 285 to 665; GenBank acc. no. 473375 and U13173) PepT1 sequences. 
     Demonstration of PepT1-like Transport Activity in MDCK Cells 
     As seen in FIGS. 3 and 5, MDCK cells express a canine homolog of mammalian PepT1 mRNA. Potential expression of PepT1 transport activity (H + -dependent, dipeptide inhibitable, low-affinity dipeptide transport) by confluent MDCK cells was evaluated using whole-cell transport techniques and glycylsarcosine (GlySar) as a model dipeptide substrate. Cells were seeded at 60,000 cells/cm 2  into 24-well trays that had been coated with rat tail collagen or poly-L-lysine and cultured (95%O 2 :5% CO 2  at 37° C.) for 3 d in media consisting of Dulbecco&#39;s Modified Eagle Medium/10% fetal calf serum/1% antimicrobial antibacterial medium. Absorption (pmols/mg protein) of [ 3 H]-glycyl-L-sarcosine (GlySar, 6 mCi/mL, Moravek Biochemicals, Brea, Calif.) was determined using the 24-well cluster tray method and representative scintillation counting. Before transport, cells were incubated at 37° C. for 30 min in 25 mM Hepes/Tris (pH 7.5), 140 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl 2 , 0.8 mM MgSO 4 , and 5 mM glucose (uptake buffer) to normalize intracellular amino acid and peptide pools. Transport was initiated by the addition of 0.25 mL of uptake buffer that contained 2.88 μm GlySar. After 30 min of uptake at 37° C., transport was terminated by rapid washing of cells with 4×2 mL 4° C. uptake buffer. Cellular protein was precipitated with 10% trichloroacetic acid and the supernatant recovered and counted to determine radioactivity ( 3 H) content. Cellular protein was then solubilized in 0.2 N NaOH and 0.2% SDS and quantified by the Lowry procedure, using bovine serum albumin as a standard. The amount of H + -dependent GlySar absorbed was calculated as the difference between uptake in pH 6.0 and pH 7.5 uptake buffers. The amount of competitor substrate-inhibitable GlySar uptake was calculated as the quotient of GlySar uptake in the absence and presence of 10 mM competitor substrate (dipeptide or amino acid) multiplied by 100%. 
     GlySar uptake in the presence of an intracellularly H +  gradient (extracellular pH of 6.0) was 2.3-fold higher in cells plated on collagen, and 1.7-fold higher when grown on poly-L-lysine, than uptake in pH 7.5 medium (Table 1). H + -dependent uptake of GlySar by MDCK cells was inhibited by 88 or 92% by the presence of 10 mM LeuTrp or TrpLeu when grown on collagen, and 87 or 92% when grown on poly-lysine, respectively (Table 1). 
     
       
         
               
             
               
               
               
               
               
             
               
             
               
               
               
               
               
             
               
             
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 Influence of extracellular pH and competitor substrates on uptake of 
               
               
                 [ 3 H]-glycylsarcosine by MDCK cells cultured on collagen- or 
               
               
                 poly-L-lysine-coated trays. 
               
               
                 Cells were cultured as described in text and uptake compared in 
               
               
                 pH 7.5 or 6.0 media that contained 2.88 [ 3 H]-glycylsarcosine 
               
               
                 for 30 min. 
               
             
          
           
               
                   
                   
                   
                 Glysylsarcosine 
                 % 
               
               
                   
                   
                   
                 uptake pmol 
                 inhibition 
               
               
                   
                 Extracellular 
                 Competitor 
                 30 min −1  mg −1   
                 of pH 6.0 
               
               
                 n 
                 pH 
                 substrate (mM) 
                 protein 
                 uptake 
               
               
                   
               
             
          
           
               
                 Collagen-coated 
               
             
          
           
               
                 5 
                 7.5 
                 none 
                 19.9 ± 2.80 
                 na 1   
               
               
                 5 
                 6.0 
                 none 
                 65.3 ± 7.95 
                 100 
               
               
                 5 
                 6.0 
                 LeuTrp (10) 
                 7.68 ± 1.37 
                 11.7 
               
               
                 5 
                 6.0 
                 TrpLeu (10) 
                 5.21 ± 0.39 
                 8.0 
               
               
                 4 
                 6.0 
                 Leucine (10) 
                 63.0 ± 4.00 
                 96.3 
               
             
          
           
               
                 Poly-L-lysine-coated 
               
             
          
           
               
                 4 
                 7.5 
                 none 
                 15.52 ± 1.06  
                 na 
               
               
                 5 
                 6.0 
                 none 
                 42.31 ± 4.03  
                 100 
               
               
                 5 
                 6.0 
                 LeuTrp (10) 
                 5.50 ± 0.58 
                 13.0 
               
               
                 5 
                 6.0 
                 TrpLeu (10) 
                 3.44 ± 0.27 
                 8.1 
               
               
                 4 
                 6.0 
                 Leucine (10) 
                 41.93 ± 2.70  
                 100 
               
               
                   
               
               
                   1 na, not applicable  
               
             
          
         
       
     
     To preliminarily characterize the kinetic parameters of peptide transport by MDCK cells, the uptake of GlySar in media that contained pH 6.0 and 0.00064, 0.0025, 0.010, 0.04, 0.160, 0.640, 2.56, or 10.2 mM of GlySar was measured (FIG.  6 ). Total GlySar uptake was by a relatively low-affinity mechanism (apparent K m  of about 4.0 mM) and high uptake velocity. Collectively, these characteristics of GlySar uptake are consistent with functional activity of PepT1 expressed by other species, as opposed to high-affinity, H + -dependent uptake by PepT2 (μm K m ). Accordingly, it is concluded that MDCK cells possess PepT1-like activity, consistent with detection of PepT1 mRNA by RT-PCR (FIGS. 1,  2 ,  5 ) and Northern blot analyses (FIGS. 3,  4 ). 
     Summary of Example 1 
     Separate partial-length canine PepT1 cDNAs (cPepT1) were generated by RT-PCR analyses from dog small intestinal epithelium (n=2; FIGS. 1,  2 ) and immortalized canine kidney cells (MDCK cells, n=1). The MDCK cDNA was sequenced (FIG. 5) and found to share 79 to 87% sequence identity with PepT1 mRNA expressed by other mammalian species. Northern blot analyses using the intestinal epithelium-derived RT-PCR cDNA confirmed expression of canine PepT1 (cPepT1) by dog tissues (liver, n=3; kidney, n=3; small intestine n=1) and MDCK cells (n=2). The identification of mRNA transcripts corresponding to PepT1 using partial-length canine-derived PepT1 cDNA (FIG. 3) was confirmed by hybridization to full-length rabbit cDNA (FIG.  4 ). Characterization of GlySar uptake by MDCK cells demonstrated that MDCK cells express PepT1-like activity (Table 1, FIG.  5 ), confirming detection of PepT1 mRNA expression by MDCK cells and use of MDCK cells as a model to characterize the function of canine PepT1. 
     EXAMPLE 2 
     Experimental Model of MDCK Cells for Evaluating the Effects of Various Peptide and Drug Substrates, and Hormones and/or Growth Factors, on the Expression of PepT1 Activity 
     Example 1 above showed that (1) a canine homolog of PepT1 (cPepT1) mRNA cloned from epithelia of the mid small intestine (jejunum) shares high sequence identity with PepT1 expressed by several other species, (2) canine liver, kidney, and jejunal epithelium express a similar pattern of cPepT1 mRNA, and (3) MDCK cells are capable of H + -dependent peptide uptake. Accordingly, MDCK cells are an appropriate model to evaluate the biochemical characteristics of cPepT1. The specific goals of this research were to (1) characterize the functional activity of low-affinity H + -dependent GlySar uptake (PepT1 activity) by MDCK cells and (2) identify di- and tripeptides that are well recognized by cPepT1 (cPepT1), especially those that contain tryptophan and leucine. 
     Previous research (Brandsch et al., 1994, Biochem J. 299:253-260) briefly reported that H + -dependent peptide uptake by MDCK cells was greater when cells were grown in a medium that contained lactalbumin hydrolysate (LHM) versus one that contained free amino acids (DMEM). Therefore, in an attempt to establish the most sensitive model possible for evaluating peptide transport systems in MDCK cells, the potential influences of LHM (peptide-containing) versus DMEM (peptide-lacking) media, and subconfluent versus confluent initial cell plating densities were compared. MDCK cells were seeded at either 60,000 cells/well (subconfluent) or 120,000 cells/well (confluent) in DMEM and, after 1 d, cultured in DMEM or LHM media for 1, 2, 3, or 5 d. The amount of protein (index of cell growth) and GlySar uptake (index of peptide uptake capacity) expressed by each well of cells was then determined. As seen in FIG. 7, the amount of cellular protein increased (P&lt;0.05) for both seeding densities and media with time of culture. A time×media interaction was observed, which reflects the greater protein content of cells grown in DMEM at day 6, as compared to those grown in LHM. At days 2, 3, or 4, however, no difference in protein content was observed. 
     The uptake of [ 3 H]-GlySar (2.88 μM, 5 μCi/mL) by the MDCK cells described in FIG. 7 was measured in the presence (pH 6.0 uptake buffer) and absence (pH 7.4 uptake buffer) of an extracellular-to-intercellular H +  (proton) gradient. A representative graph (FIG. 8) compares the uptake of GlySar by cells seeded at 60,000/well and cultured in the LHM or DMEM. For both culture media, GlySar uptake in the presence of pH 6.0 was greater (P&lt;0.01) than that in pH 7.4 buffer and displayed a quadratic (P&lt;0.01) response to length of culture, reflecting a buffer×day of culture interaction (P&lt;0.01). DMEM-cultured cells seeded at 120,000/well displayed almost identical uptake characteristics as just described for cells seeded at 60,000/well. In contrast, GlySar uptake in the presence of pH 6.0 buffer at day 3 by LHM-cultured cells was only 28% larger (quantitatively) than that observed by DMEM-cultured cells seeded at 60,000/well. 
     To further refine the analysis of media influence on the peptide transport capacity of MDCK cells plated at 60,000 or 120,000 cells per well, the H + -dependent GlySar uptake was calculated as the arithmetic difference between uptake in pH 6.0 and pH 7.4 buffers (FIG.  9 ). Despite the comparable protein contents of cells observed at day 3 (FIG.  7 ), cells seeded at 60,000 and grown in LHM media demonstrated about 60% greater capacity for GlySar uptake as did cells grown in DMEM (FIG. 9; day×media interaction, P&lt;0.01). For all cells, the capacity for GlySar uptake per mg of cellular protein was decreased at day 6. This difference was the result of a lesser uptake at pH 6.0 by the LHM-cultured cells, and not the result of a larger pH 7.4 uptake. 
     The results of this experiment indicate that culturing cells in media that contains peptides does not increase growth rate but does increase the capacity for peptide uptake if cells are seeded at 60,000/well and cultured for 2 days in LHM. As such, these data are consistent with the induction of PepT1 expression by culture peptide-containing medium and describe an optimal set of culture conditions for characterizing H + -dependent peptide transport activity of the canine PepT1 transporter. These data also confirm, and more thoroughly describe, the stimulating effect of LHM versus DMEM media on peptide transport proteins that was initially reported by Brandsch et al. (1994). 
     Using the maximal uptake-stimulating culture parameters determined in Experiment 3, the effect of an extracellular-to-intracellular pH gradient on GlySar uptake was further evaluated to determine a pH level at which maximal GlySar uptake could be achieved, but which would replicate physiologic conditions (FIG.  10 ). As expected, the presence of a pH gradient stimulated (P&lt;0.001) H + -dependent GlySar uptake, in a quadratic (P&lt;0.01) fashion. Uptake at pH 5.5 or 6.0 was about 2.7 times greater than that achieved at pH 7.5. These results are consistent with the data in FIGS. 8 and 9 and known H + -dependence of mammalian peptide transport proteins. Accordingly, the use pH 6.0 buffers for the characterization of H + -dependent GlySar uptake was incorporated into the standard experimental conditions. 
     To determine the appropriate time period to measure initial (linear) rates of GlySar uptake, a by-minute time-course experiment was performed. As seen in FIG. 11, H + -dependent GlySar (100 uM) uptake increased linearly for 1 h and then slowed (quadratic response, P&lt;0.01). GlySar uptake in pH 6.0 buffer at 3.75, 7.5, 15, 30, 60 and 120 min was about 2, 2.1, 2.25, 2.65, 2.79, and 2.62 times more (P&lt;0.001), respectively, than uptake from pH 7.4 buffer. Because uptake was proportional to time of uptake through 1 h, future experiments were conducted using a 30-min time period. 
     To confirm that H + -dependent GlySar uptake was saturable, and therefore mediated, the uptake of GlySar from pH 6.0 and 7.4 uptake buffers containing 0.025, 0.1, 0.4, 1.6, 6.4, or 25.6 mM GlySar was evaluated (FIG.  12 ). Uptake of GlySar was greatest (P&lt;0.001) from the pH 6.0 buffers, at all concentrations. H + -dependent GlySar uptake was saturable, consistent with an apparent K m  for GlySar of about 1.1 mM. These values are consistent with our preliminary trials that estimated a K m  of 1.1 mM for GlySar uptake by MDCK cells using only pH 6.0 uptake buffer and indicate that H + -dependent GlySar uptake is predominately, if not completely, a result of low affinity (mM) H + /peptide cotransporter activity (PepT1). As a comparative value, the reported K m  of for GlySar uptake by the PepT1-expressing Caco-2 cells also is 1.1 mM. It is of interest also to note that GlySar uptake in the absence of a pH gradient (pH 7.4 buffers) also displayed linear (P&lt;0.01) and quadratic (P&lt;0.001) components, (1) reflects that the pH “7.4” buffer was in fact slightly acidic, (2) represents the activity of the putative basalateral peptide transporter running in “reverse”, or (3) indicates the presence of a non-characterized peptide transport system. As a result of this experiment, subsequent H + -dependent peptide transport trials were conducted using 100 μM GlySar, a value well below the K m  but one that will result in increased transport activity, and thus, sensitivity. 
     Characteristic hallmarks of low affinity H + /peptide cotransport activity, classically defined using membrane vesicles of several species, and more recently by functional expression studies using human, rat, and rabbit PepT1 cDNA, is the recognition of some, but not all, β-lactam antibiotics. In addition, PepT1 recognition of cefadroxil is low (the K l  of cefadroxil inhibition of GlySar uptake by PepT1 is 3 mM), whereas recognition of cefadroxil by PepT2 is high (the K i  of cefadroxil inhibition of PepT2 transport of GlySar is 30 μM). To determine whether MDCK cPepT1 activity shared these functional features, the uptake of 100 μM GlySar in the absence and presence of pH 7.5 and pH 6.0 buffer, and, in pH 6.0 buffers, the presence of 1 mM additional GlySar (self-inhibitor control), 3 mM Penicillin-G, 30 μM cefadroxil, or 3 mM cefadroxil was compared (FIG.  13 ). H + -dependent GlySar uptake was not inhibited by penicillin-G or 30 μM cefadroxil, but was inhibited about 76% by 3 mM cefadroxil. As expected, the presence of 1 mM GlySar self-inhibited 100 μM GlySar uptake by 64%. These results indicate that H+-dependent uptake of GlySar by MDCK cells is by PepT1 activity. 
     Other hallmarks of PepT1 function are the decreased ability of Gly-containing peptides to inhibit GlySar, in proportion to their length, and sensitivity to inhibition by carnosine (β-Ala-His). To determine if cPepT1 activity behaves as reported for other PepT1 activities, the relative abilities of 1 mM Gly ([ 3 H]-Gly free amino acid control), GlyGly, [Gly] 4 , or [Gly] 5  to inhibit H + -dependent 100 μM GlySar was determined (FIG.  14 ). Gly (5.0%) and [Gly] 5  (7.3%) did not influence uptake, whereas GlyGly inhibited and [Gly] 4  tended to inhibit uptake by 63 and 23%, respectively. This pattern of Gly-containing peptides to inhibit GlySar uptake in an inverse proportion to the number of glycyl residues in the canine MDCK cell model is consistent with PepT1 activities reported for other species. Similarly, GlySar uptake was inhibited 50% by 1 mM carnosine (data not shown but listed in Table 2 below). 
     Together with the molecular identification of PepT1 mRNA expression in MDCK cells using full-length rabbit cDNA and our canine RT-PCR product (See Example 1 data), the above biochemical characterization data indicate that H + -dependent GlySar uptake activity in MDCK cells is consistent with the low-affinity, high-capacity of the PepT1 transport protein. Collectively, the above experiments resulted in the generation of an experimental regimen for the culture and determination of H + -dependent peptide transport activity in MDCK cells, with which to evaluate the relative substrate preferences of canine PepT1 (cPepT1). 
     Accordingly, the following general regimen was used to perform a series of experiments that evaluated the relative abilities of candidate di-(primarily) and tri-peptides to inhibit GlySar uptake by endogenously expressed cPepT1 in MDCK cells: 
     1. Sixty thousand cells/well were plated into collagen-coated 24-well trays and cultured at 37° C. in an atmosphere of 95% air/5% CO 2  in DMEM/10% FCS that contained antibiotics for 1 day. 
     2. The media was removed and cells were cultured in LHM/10% FCS/antibiotics for 1 day. 
     3. The media was removed and cells cultured in LHM/10% FCS (no antibiotics) for 20 h. 
     4. The media was removed and cells cultured for 30 min in air at 37° C. in depletion medium (25 mM Hepes/Tris (pH 7.5), 140 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl2, 0.8 mM MgSO4, and 5 mM glucose, to normalize intracellular nutrient pools. 
     5. Transport was initiated by replacing depletion medium with uptake medium (depletion medium adjusted to pH 6.0 or kept at pH 7.4) that contained 100 μM GlySar (at a specific activity of 5 μCi/mL, with [ 3 H]-GlySar supplying 2.88% of total GlySar substrate) and (or) 1 mM of inhibiting peptide. 
     An inhibitory substrate concentration of 1 mM was selected because the literature indicates that typical K m  values for PepT1 ranges from 0.5 to 5 mM. Therefore, by selecting an inhibitor concentration of 1 mM (not expected to completely inhibit uptake), our goal was to more finely delineate the relative abilities of candidate inhibitors than if the typical 5 mM inhibitor concentration (expected to achieve close to 100% inhibition of GlySar uptake) was used. Candidate peptides were selected based on their containing Trp, Leu, Met, and (or) Arg, substrates. In total, 23 inhibitory peptides and 2 drug compounds were screened using this protocol. 
     To determine the potential of Trp and Leu absorption as dipeptides by cPepT1, the ability of TrpLeu versus LeuTrp dipeptides to inhibit 100 μM GlySar uptake was evaluated (FIG.  15 ). The presence of either TrpLeu or LeuTrp in the pH 6.0 uptake buffer abolished H + -dependent GlySar uptake by 117% or 114%, respectively. In contrast, neither Leu nor Trp significantly influenced H + -dependent GlySar uptake. These results indicate that a lesser concentration of inhibitor would be required to delineate the relative recognition of TrpLeu and LeuTrp by cPepT1. With regard to the mechanism of H + -independent GlySar uptake observed throughout these experiments, it is of interest to note that TrpLeu and LeuTrp inhibited H + -independent GlySar uptake by 36% and 46%, respectively. 
     To further evaluate the potential of Trp to be absorbed in the form of peptides by cPepT1, the ability of TrpTrp, TrpGly, and TrpGlyGly to inhibit GlySar uptake was compared (FIG.  16 ). As observed for TrpLeu (FIG.  15 ), TrpTrp abolished H + -dependent GlySar uptake and inhibited H + -independent uptake by about 22%. TrpGly abolished H + -dependent GlySar uptake but did not influence H + -independent uptake. The tripeptide TrpGlyGly also significantly inhibited GlySar uptake, but to a lesser extent (73%) than did TrpTrp or TrpGly. 
     To determine the relative potential of other amino acids (Met, Arg, Lys, Phe, for example) to be absorbed in the peptide-bound form, additional GlySar competitive inhibition experiments were conducted using the above-described regimen and a variety candidate peptides at 1 mM. The results of these experiments are summarized in Table 2, which also includes those experiments described in FIGS. 13,  14 ,  15 , and  16  for comparative purposes. 
     
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 Influence of 1 mM extracellular peptides and antibiotics on 100 μM 
               
               
                 glycylsarcosine (GlySar) uptake 1  by MDCK cells. 
               
             
          
           
               
                   
                 Extracellular 
                 % inhibition of 
                   
               
               
                   
                 Substrate 
                 H + -dependent 
               
               
                   
                 (1 mM) 
                 GlySar uptake 4   
                 n 
               
               
                   
                   
               
             
          
           
               
                   
                 Positive control (model) substrates 
                   
                   
               
               
                   
                 GlyGly 
                 89 
                 8 
               
               
                   
                 [Gly] 4   
                 19 
                 8 
               
               
                   
                 [Gly] 5   
                 9 
                 8 
               
               
                   
                 Carnosine (β-AlaHis) 
                 50 
                 8 
               
               
                   
                 Penicillin-G 
                 0 
                 8 
               
               
                   
                 Cefadroxil 2   
                 0 
                 6 
               
               
                   
                 Cefadroxil 3   
                 59 
                 5 
               
               
                   
                 Treatment substrates 
               
               
                   
                 100% inhibition 
               
               
                   
                 GlnGln 
                 100 
                 8 
               
               
                   
                 GlyLeu 
                 115 
                 8 
               
               
                   
                 GlyMet 
                 114 
                 8 
               
               
                   
                 LeuMet 
                 114 
                 8 
               
               
                   
                 LeuTrp 
                 113 
                 8 
               
               
                   
                 MetLeu 
                 122 
                 8 
               
               
                   
                 MetMet 
                 100 
                 8 
               
               
                   
                 MetPhe 
                 100 
                 8 
               
               
                   
                 MetPro 
                 100 
                 8 
               
               
                   
                 TrpLeu 
                 116 
                 8 
               
               
                   
                 TrpTrp 
                 119 
                 7 
               
               
                   
                 &lt;100% inhibition 
               
               
                   
                 GlnGlu 
                 83 
                 8 
               
               
                   
                 MetGlu 
                 93 
                 8 
               
               
                   
                 MetLys 
                 88 
                 8 
               
               
                   
                 TrpGly 
                 88 
                 7 
               
               
                   
                 MetGlyMetMet (SEQ ID NO:10) 
                 50 
                 8 
               
               
                   
                 TrpGlyGly 
                 33 
                 7 
               
               
                   
                 LeuArg 
                 32 
                 8 
               
               
                   
                 ArgLeu 
                 32 
                 8 
               
               
                   
                   
               
               
                   
                   1 pmol mg −1  protein 30 min −1   
               
               
                   
                   2 30 μM  
               
               
                   
                   3 3 mM  
               
               
                   
                   4 That portion of the % inhibition value that exceeds 100, likely represents the ability to inhibit H + -independent GlySar uptake.  
               
             
          
         
       
     
     The inhibitors are listed within groupings in order of their relative ability to inhibit 100 μM of GlySar uptake. In addition to the listed peptides, the constituent free amino acids were tested within the appropriate experiment to evaluate whether the peptide-bound or free amino acid was responsible for any affect on GlySar uptake. As expected, the presence of 1 mM constituent free amino acid did not influence GlySar uptake. Inhibition percentages of 50% indicate that the inhibitor substrate was recognized at least as well as was GlySar, given that the K m  of GlySar was determined to be about 1 mM (FIG. 12) and that the substrate was present at 1 mM. Of the 19 treatment peptides evaluated, eleven abolished H + -dependent GlySar uptake, with seven of these also displaying the ability to inhibit H + -independent GlySar uptake. Of the remaining eight peptides tested, four displayed greater than 80% inhibition while four inhibited GlySar uptake by 50% or less. These results indicate that a wide variety of peptides of nutritionally important constituent amino acids are recognized by cPepT1. 
     Overall, the observation that cPepT1 activity was sensitive to a number of substrates is typical of PepT1 function. However, what was surprising was the large number of peptides that completely inhibited GlySar uptake. To establish a more sensitive relative inhibitory order among peptides that inhibited GlySar uptake by more than 80%, and, therefore, a more accurate potential for recognition, fourteen peptides were re-screened for their ability to inhibit 100 μM GlySar uptake using the same cell culture and transport regimen but using only 10% of the previous inhibitor concentration (100 μM). The data from an experiment to directly compare the ability of 100 μM Trp-containing peptides are shown in FIG.  17 . All Trp-containing peptides inhibited H + -dependent GlySar uptake. However, TrpLeu inhibited more (92%) than did LeuTrp (58%), TrpTrp (62%), or TrpGly (45%). These values and the results of other experiments comparing the relative ability of Leu-, Met-, and Arg-containing peptides are listed in Table 3. 
     
       
         
               
             
               
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Influence of 100 μM extracellular peptides on 100 μM glycylsarcosine 
               
               
                 (GlySar) uptake 1  by MDCK cells. 
               
             
          
           
               
                   
                 Extracellular 
                 % inhibition 
                   
               
               
                   
                 substrate 
                 of H + -dependent 
               
               
                   
                 (100 μM) 2   
                 GlySar uptake 
                 n 
               
               
                   
                   
               
             
          
           
               
                   
                 Trp-containing peptides 
                   
                   
               
               
                   
                 TrpLeu 
                 92 
                 8 
               
               
                   
                 TrpTrp 
                 62 
                 8 
               
               
                   
                 LeuTrp 
                 58 
                 8 
               
               
                   
                 TrpGly 
                 45 
                 8 
               
               
                   
                 Leu-containing peptides 
               
               
                   
                 TrpLeu 
                 94 
                 8 
               
               
                   
                 LeuMet 
                 80 
                 8 
               
               
                   
                 MetLeu 
                 77 
                 8 
               
               
                   
                 GlyLeu 
                 65 
                 8 
               
               
                   
                 Met-containing peptides 
               
               
                   
                 MetMet 
                 85 
                 8 
               
               
                   
                 MetPhe 
                 84 
                 8 
               
               
                   
                 MetGlu 
                 31 
                 8 
               
               
                   
                 MetLys 
                 30 
                 8 
               
               
                   
                 Arg-containing peptides 
               
               
                   
                 ArgLeu 
                 49 
                 8 
               
               
                   
                 LeuArg 
                 8.9 
                 8 
               
               
                   
                 ArgTrp 
                 8.9 
                 8 
               
               
                   
                   
               
               
                   
                   1 pmol mg −1  protein 30 min −1   
               
               
                   
                   2 Data are grouped by experiment  
               
             
          
         
       
     
     Overall, four of the peptides inhibited GlySar uptake by at least 80%, six by more than 40%, and four less than 40%, thus establishing a relative ranking for recognition by cPepT1. Among the five Trp-containing peptides (FIG. 17, Table 3), TrpLeu demonstrated the greatest ability to inhibit GlySar uptake. TrpLeu also demonstrated the greatest ability to inhibit GlySar uptake (94%) among the Leu-containing peptides. Among the Met-containing substrates that were directly compared within the same experiment, the neutral peptides, MetMet and MetPhe, inhibited more GlySar uptake than did the anionic (MetGlu) or cationic (MetLys) carboxyl residues. Interestingly, as a group the Arg peptides demonstrated the least inhibitory ability, seemingly in keeping with the apparent lesser recognition by PepT1 of substrates with charged residues. However, it is of interest to note that 100 μM ArgLeu demonstrated a much greater ability to inhibit GlySar uptake than did LeuArg (49 versus 8.9%). 
     To confirm the relative ranking of TrpLeu&gt;TrpTrp inhibition of GlySar (Tables 2 and 3), Michaelis-Menton constants for substrate inhibition (K i ) of GlySar uptake by TrpLeu and TrpTrp were generated by graphical analyses of IC 50  experiments (FIG.  18 ). In keeping with the results achieved in the 100 μM-inhibition studies, TrpLeu inhibited GlySar uptake at lower concentrations than did TrpTrp (K l =0.2 versus 0.75 μM, respectively). 
     Collectively, the results of cPepT1 competitive inhibition trials using MDCK cells indicate that TrpLeu is better recognized by cPepT1 than any other tested peptide. The results also indicate that a number of Trp-, Leu, and Met-containing peptides also are well recognized by cPepT 1. Ultimately, in the intestinal environment, it is the combination of recognition by the transporter and relative resistance of the peptide to luminal and membrane-bound peptidases that will determine how much of a given peptide will be absorbed. In this regard, there is some evidence to suggest that Gly-X peptides are more resistant than other peptides, especially by blood and renal peptidases. If so, then GlyLeu may be a better candidate substrate than TrpLeu to supply Leu. Similarly, tripeptides, as a group, are thought to be relatively resistant to hydrolysis. Thus, more TrpGlyGly may prove to be absorbed in larger amounts by the intestine than TrpLeu. 
     An important result of this set of experiments was the establishment of a sensitive experimental regimen/model to evaluate potential affecters of peptide transport capacity. Accordingly, this experimental model of MDCK cells grown in LHM affords an opportunity to evaluate the effects of various peptide and drug substrates, and hormones and (or) growth factors, on the expression of PepT1. 
     Thus, the culture of MDCK cells in LHM versus DMEM results in an increase of H + -dependent GlySar uptake (K m =1.1 mM) that is consistent with mammalian PepT1-like activity. Using this stimulated model, the ability of twenty-three di- and tripeptides at 1 mM, and fourteen at 100 μM, extracellular concentrations were screened for their ability to inhibit 100 μM GlySar uptake, as an indicator of recognition by PepT1. Of the Trp- and (or) Leu-containing peptides evaluated, TrpLeu (K i =0.2 μM) and LeuTrp (K l =0.75 μM) demonstrated the greatest ability to inhibit GlySar uptake, with TrpLeu demonstrating a relatively higher affinity (lower K l ) for PepT1. Of the Met-containing peptides evaluated, four (MetMet, MetPhe, LeuMet, MetLeu) appear particularly well recognized by PepT1. In contrast, as a group, Arg-containing peptides displayed the least inhibition of PepT1 activity. Overall, these results indicate that ePepT1 is capable of recognizing a variety of di- and tripeptides, including, for example, those that contain leucine and tryptophan. 
     EXAMPLE 3 
     Experimental Model to Determine whether the H + /peptide Transport Capacity Expressed by MDCK Cells Is Sensitive to Substrate Regulation 
     Trial 1 
     Examples 1 and 2 above demonstrated that Madin-Darby canine kidney (MDCK) cells express PepT1 mRNA and characterized H + -dependent biochemical properties. Therefore, MDCK cells were chosen as the experimental model to determine whether the H + /peptide transport capacity expressed by MDCK cells is sensitive to substrate regulation. Research from Example 2 demonstrated that MDCK cells grown in lactalbumin hydrolysate medium (LHM) had elevated levels of peptide uptake capacity. Accordingly, to avoid potential confounding effects of the peptide-containing LHM and individual treatment peptides, DMEM (contains no peptides) and not LHM was selected as the appropriate medium to test the influence of extracellular peptides on canine PepT1 functional capacity of MDCK cells. GlyPhe was selected as a substrate because it has been reported to increase brush border membrane content of PepT1, (Shiraga T, Miyamoto K, Tanaka H, Yamamoto H, Taketani Y, Morita K, Tamai I, Tsuji A, Takada E. Cellular and molecular mechanisms of dietary regulation on rat intestinal H+/peptide transporter PepT1 . Gastroenterology  1999; 116:354-362), whereas Phe and Gly were tested as constituent free amino acid treatment controls. Carnosine was selected because of its high content in meat-based diets. 
     Cell culture. 
     All cells were plated (60,000/2 cm 2  well) and cultured (95% air/5% CO 2 , 37° C.) for 24 h in Dulbecco&#39;s Modified Eagle Media/10% fetal calf serum (FCS)/1%Antibiotic/Antimicrobial solution (ABAM) (DMEM media). Following these initial common culture conditions, cells then were cultured in DMEM, or DMEM that contained 10 mM of Camosine, GlyPhe, Phe, or Gly. Media were changed every 24 h. Media treatments (n=8) were as follows: 
     DMEM 
     DMEM+10 mM Carnosine 
     DMEM+10 mM GlyPhe 
     DMEM+10 mM Phe 
     DMEM+1mM G1 
     Uptake measurements. 
     The measurement of [ 3 H]Glysarcosine uptake was performed by using a 24-well cluster tray method (Kilberg M S. Measurement of amino acid transport by hepatocytes in suspension and monolayer culture.  Methods Enzym  1989; 173:564-575. Matthews J C, Aslanian A, McDonald K K, Yang W, Malandro M S, Novak D A, Kilberg M S. An expression system for mammalian amino acid transport using a stably maintained episomal vector.  Anal Biochem  1997; 254:208-214), and used in Examples 1 and 2. Cells were cultured for 30 min in air at 37° C. in depletion medium (25 mM Hepes/Tris (pH 7.5), 140 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl2, 0.8 mM MgSO 4 , and 5 mM glucose), to normalize intracellular nutrient pools before transport. The transport assays are initiated by replacing depletion medium with uptake medium (Depletion medium adjusted to pH 6.0) that contained 100 μM GlySar (5 μCi/mL, with [ 3 H]-GlySar supplying 2.88% of total GlySar). After a 30 minute incubation period, transport was terminated with four rinses of 4° C. depletion medium (pH 7.5). Two hundred and twenty μL of 10% trichloroacetic acid was added to each well, and the radioactivity of the supernatant quantified by liquid scintillation counting. The cells of each well are solubilized in 0.2 N NaOH/0.2% SDS and the protein quantified by using the modified Lowry assay, using bovine serum as a standard. Id. Peptide uptake will be reported as pmol* mg −1  protein* 30 min −1 . Uptake measurements were taken after 24, 48, and 72 hours of culture in treatment media. 
     Results. 
     The previous research characterizing H + -dependent peptide transport by MDCK cells (Example 2 above) clearly showed that transport velocity is dependent on protein content. Therefore, to make a valid comparison of various treatment parameters on GlySar uptake, the protein content of compared treatment groups must not differ. Accordingly, the influence of culture media on MDCK cellular protein was evaluated (FIG.  19 ). All media treatments supported cellular growth from 1 to 3 d and no difference in protein content among treatments was observed. Similarly, no difference in uptake velocity (capacity) was observed among treatment groups, for any culture period (FIG.  20 ). 
     Trial 2 
     The results from Trial 1 suggest that either canine PepT1 is not sensitive to substrate regulation or that the substrates and(or) stimulation time were inadequate to influence H + -dependent peptide uptake in MDCK cells. Again, DMEM was selected as the basal medium to allow the effect of individual peptides on peptide transport activity to be evaluated. To evaluate the latter two possibilities, a second trial was conducted that included a culture period of 9 d. GlySar was added as another potential affecter of H + -dependent peptide transport capacity because 10 mM GlySar it is reported capable of stimulating increased PepT1 activity (Adibi S. The oligopeptide transporter PepT1 in human intestine: biology and function.  Gastroenterology  1997; 113:332-340) in Caco-2 cells. GlyPro was added as a treatment because of its high content in muscle tissue, thus is likely to be abundant in meat-based diets. 
     Cell culture. 
     The MDCK cell line was maintained as described previously in the Methods section of Trial 1. Following initial and common culture conditions, cells were cultured in DMEM, or DMEM that contained 10 mM GlySar, GlyPro, GlyPhe, or Carnosine. Media were changed every 24 h. Media treatments (n=8) were as follows: 
     DMEM 
     DMEM+10 mM GlySar 
     DMEM+10 mM GlyPro 
     DMEM+10 mM GlyPhe 
     DMEM+10 mM Carnosine 
     Uptake measurements. 
     The measurement of [ 3 H]Glysarcosine uptake was performed by using the 24-well cluster tray method as previously described in the Methods section of Trial 1. Peptide uptake will be reported as pmol* mg −1  protein* 30 min −1 . Uptake measurements were taken after 4, 12, 24, 36, 72, 120, 168, and 216 hours of culture in treatment media. 
     Results. 
     Protein content in all treatment groups increased linearly from 4 to 216 h (9 d) of culture, for all treatment groups (FIG.  21 ). However, within a culture period, protein contents of treatment groups did not differ. Over the 216-h culture period, protein increased about 4.5 times, from about 40 to 220 μg/well. In contrast to Trial 1 results, media treatment did influence GlySar uptake capacity (FIG.  22 ). In addition, a treatment×time effect was observed that represents differences in the time of culture required for GlySar and carnosine treatment stimulation of GlySar uptake capacity. Specifically, GlySar containing DMEM culture treatment resulted in an increase in GlySar uptake capacity of about 30% over DMEM control media by 24 h of culture time. This level of increase was maintained through 216 h. In contrast, culture in carnosine-containing media did not result in a significant (23%) increase of GlySar uptake capacity over that by DMEM-cultured cells until 72 h of culture. This stimulation then steadily increased to 291% over 216 h of culture. The nature of stimulated uptake between the two peptide substrates also differed. That is, the magnitude of carnosine-stimulated GlySar uptake was essentially constant from 72 to 216 h, whereas that for GlySar culture decreased during this period. Collectively, these data indicate that H + -dependent peptide transport in cultured MDCK cells can be stimulated by at least two of PepT1 substrates, GlySar and carnosine. 
     Trial 3 
     The data from Trial 2 indicate that H + -dependent GlySar uptake capacity by fed MDCK cells can be upregulated by the inclusion of 10 mM GlySar for at least 24 h and 10 mM carnosine for at least 72 h. It is of equal interest to understand if H + -dependent GlySar uptake capacity is sensitive to nutrient deprivation and(or) stimulation by glucocorticoids. A preliminary study indicates that fasting increases the expression of PepT1 in rat small intestine epithelia. Thamotharan M, Bawani S, Zhou X, Adibi S. Functional and molecular expression of intestinal oligopeptide transporter (PepT1) after a brief fast.  Metabolism  1999; 48:681-684. 
     To initiate investigation of potential influence of fasting and glucocorticoids on MDCK cells expression of GlySar uptake capacity, the H + -dependent uptake of GlySar was evaluated over a 72 period of nutrient deprived or fed and cultured with dexamethasone (Dex) and compared to that by cells cultured in DMEM or DMEM that contained insulin (negative control) (Trial 3A). The “nutrient deprived” treatment actually contained 5 mM glucose and appropriate salts to ensure adequate basal metabolic conditions. 
     Although recruitment of PepT1 protein and activity appears sensitive to insulin-stimulated recruitment from cytosolic vesicles in Caco-2 cells (Thamotharan M, Bawani S, Zhou X, Adibi S. Hormonal regulation of oligopeptide transporter PepT1 after a brief fast.  Am J Physiol  1999; 276:C821-826, MDCK cells are reported to be insensitive to insulin, likely as an inability to express the insulin receptor. Hofmann C, Crettaz M, Bruns P, Hessel P, Hadawi G. Cellular responses elicited by insulin mimickers in cells lacking detectable plasma membrane insulin receptors.  J Cell Biol  1985; 27:401-414. In contrast to the lack of insulin sensitivity, IGF-I is known to stimulate DNA synthesis and cell proliferation in MDCK cells. Sukegawa I, Hizuka N, Takano K, Asakawa K, Shizume K. Characterization of IGF-1 receptors on MDCK cell line.  Endocrinol Japan  1987; 34(3):339-346. Mouzon S H, Kahn R. Insulin-like growth factor-mediated phosphorylation and proto-ontogeny induction in MDCK cells.  Mol Endocrinol  1991; 5:51-60. The understanding that MDCK cells are apparently insensitive to insulin stimulation yet are sensitive to IGF-I stimulation appears to be a paradox given that the supraphysiologic levels of both substrates employed in the perspective studies and the known ability of insulin to cross react with the IGF-I receptor. Accordingly, another trial (Trial 3B) was conducted to evaluate the influence of increasing IGF-I concentrations on H + -dependent GlySar uptake by MDCK of the same plating stock. 
     Trial 3A 
     Cell culture. 
     MDCK cells were maintained as described in Trial 1, except that cells were cultured for only 1 d before transport trials were performed. Following initial and common culture conditions, cells were cultured in a “nutrient depleted” buffer (Hepes/Tris (pH 7.5),140 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl 2 , 0.8 mM MgSO 4 ) that contained 5 mM glucose as an energy source, but that lacked amino acid or vitamin sources. In contrast, cells cultured in DMEM, or DMEM that contained 5 nM Dex, 500 nM Dex, 5 nM insulin, or 500 nM insulin, were adequately nourished. Media treatments (n=4) were as follows: 
     Nutrient Depleted 
     DHEM 
     DMEM+5 nM Dex 
     DMEM+500 nM Dex 
     DMEM+5 nM Insulin 
     DMM+500 nM Insulin 
     Uptake measurements. 
     The measurement of [ 3 H]Glysarcosine uptake was performed by using the 24-well cluster tray method as previously described in the Methods section of Trial 1. Peptide uptake is reported as pmol* mg −1  protein*30 min −1 . Uptake measurements were taken after 30 min and 4 h of culture in treatment media. 
     Trial 3B 
     Trial 3B was conducted in the same manner as described for Trail 3A, except that cells were cultured in DMEM or DMEM that contained 1 nM IGF-1, 5 nM IGF-1, 25 nM IGF-1, or 100 nM IGF-1. Uptake measurements were taken after 30 min and 4 h of culture time. Media treatments (n=4) were as follows: 
     DMEM (pH 6 measurement) 
     DMEM (pH 7.5 measurement) 
     DMEM+1 nM IGF-1 
     DMEM+5 nM IGF-1 
     DMEM+25 nM IGF-1 
     DMEM+100 nM IGF-1 
     Results. 
     Protein content of the treatments within Trails 3A or 3B did not differ. After 4 h of culture, however, the capacity for H + -dependent peptide uptake was reduced 35% in cells deprived of nutrients but adequate in energy (FIG.  23 ). In contrast, dexamethasone had no effect on GlySar uptake. As expected, and consistent with the concept that MDCK cells are insulin-insensitive, the presence of insulin for 4 h had no effect on GlySar uptake capacity. Similarly, culture of cells with increasing amounts of IGF-I elicited no significant stimulation of H + -dependent GlySar uptake (FIG.  24 ). Quantitatively, however, 1 to 25 nM of IGF-I tended to increase GlySar uptake capacity by 10 to 15%. 
     Given the noted restrictions of Trail 3, and the low number of observations (n=4) results from trial 3A and 3B suggest that H + -dependent uptake of GlySar by MDCK is sensitive to nutrient deprivation and, perhaps, IGF-I. 
     EXAMPLE 4 
     PepT1 Sequence 
     Clone12 (5 th  Round; SEQ ID NO:11) Primer Pair is GSP3-4; GSP3-1R Using Regular RT-PCR 
     
       
         catcttcttcatcgtggtcaatgagttctgtgaaaga ttttcctactatggaatgagagcactcctgattctgtacttcagacg  
       
     
     
       
         gttcatcgggtgggacgataatctgtccacgg ccatctaccacacgtttgtggctctgtgctacctgacgccgatcctcgg  
       
     
     
       
         cgcactgatcgcagactcctggctgggaaagttcaa gacaatcgtgtcactctccattgtctacacaattggacaggcggt  
       
     
     
       
         cactgcagtaagctcaattaatgacctcacagactat aacaaagatggaactcctgacaatctgtccgtgcatgtggcact  
       
     
     
       
         gtccatgattggcctggccctgatagctctgggaa ctggaggaataaagccctgtgtgtctgcatttggtggagaccagtt  
       
     
     
       
         tgaagagggccaggaaaaacaaagaaacagattc ttttccatcttttatttggccattaatgctggaagcttgatttccactat  
       
     
     
       
         tgtcactcccatgctcagagttcacgaatgt ggaatttacagtcagaaagcttgttacccactggcatttggggttcctgctg  
       
     
     
       
         ctctcatggccgtatctctgattgtatt tgtcattggcagtggaatgtacaagaagttt cagccccagggtaatgtcatgggt  
       
     
     
       
         aaagttgtcaagtgcattggttttgccct caaaaataggtttaggcaccggagtaagcagtttcccaagagggagcactgg  
       
     
     
       
         ctggactgggctaaagagaaatac gatgagcggctcatctctcaaattaagatggtcacaaaagtgatgttcttgtacatcc  
       
     
     
       
         cactcccaatgttctgggccctgtttgacc agcagggctccaggtggacactgcaagcaacagctatgagtgggaaaatt  
       
     
     
       
         ggacttcttgaagttcagccagatcagat gcagactgtgaatgccatcttgattgtcgtcatggtccccatcatggatgccg  
       
     
     
       
         tggtgtaccctctgattgcaaaatgt ggcttcaatttcacctccttgaagaggatgacagttggaatgttcctggcttccatgg  
       
     
     
       
         ccttcgtgatggcggcgattgttcagctgg aaattgataaaactcttccagtcttcccc aaacaaaatgaagtccaaatcaa  
       
     
     
       
         agtactgaatataggaaatggtgccat gaatgtatcttttcctggagcggtggtgacag ttagccaaatgagtcaatcagat  
       
     
     
       
         ggatttatgacttttgatgtagacaaac tgacaagtataaacatttcttccactggatcaccagtcattccagtgacttataact  
       
     
     
       
         ttgagcagggccatcgccataccc ttctagtatgggcccccaataattaccgagtggtaaaggatggccttaaccagaa  
       
     
     
       
         gccagaaaaagggag 
       
     
     Amplification Conditions 
     
       
         
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
                   
               
               
                   
                 Initiale 
                 Denatur- 
                 An- 
                 Amplifi- 
                   
                   
               
               
                   
                 Denaturat 
                 ation 
                 nealing 
                 cation 
                 Extension 
                 Cooling 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                 Temp 
                 94° C. 
                 94° C. 
                 55° C. 
                 72° C. 
                 72° C. 
                 4° C. 
               
               
                 Min. 
                 10 min 
                 2 min 
                 1.5 min 
                 2 min 
                 10 min 
                 inf. 
               
             
          
           
               
                 Cycle 
                 1 
                 35 
                 1 
               
               
                   
               
             
          
         
       
     
     Clone37 Beginning (6 th  Round; SEQ ID NO:12) Primer Pair Is GSP3-9; AUAP Using 3′RACE Protocol 
     gccatcgccatacccttctagtatgggccccc aataattaccgagtggtaaaggatggcc ttaaccagaagccagaaaa aggagaaaatggaatcagatttataaatagtctt aatgagagcctcaacatcaccatgggcgacaaagtttatgtgaatgtc accagtcacaatgccagcgagtatcagttctttt ctttgggcacaaaaaacattacaataagttcaacacaacagatctcac aaaattgtacaaaagttctccaatcatccaaccttgaa tttggtagtgcatatacctatgtaatcggaacgcagagcactggc tgccctgaatgcatatgtttgaagatattcaccc aacacagttaacatggctctgcagatcccgcagtacttcctcatcacc tgcggcgaggtggttttctctgtcacaggact ggagttctcatattctcaggccccctccaacatgaagtcggtgcttcagg cgggatggctgctgacagtggcttgttggcaa catcattgtgctcattgtggcaggagcaggccagttcagtgaacagtg ggctgaatacatcctatttgcggcattgctt ctggttgtctgtgtaatatttgccat catggcccggttttacacttacgtcaatc cagcagagattg 
     Amplification Conditions 
     
       
         
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
                   
               
               
                   
                 Initiale 
                 Denatur- 
                 An- 
                 Amplifi- 
                   
                   
               
               
                   
                 Denaturat 
                 ation 
                 nealing 
                 cation 
                 Extension 
                 Cooling 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                 Temp 
                 94° C. 
                 94° C. 
                 52° C. 
                 72° C. 
                 72° C. 
                 4° C. 
               
               
                 Min. 
                 10 
                 2 min 
                 1.5 min 
                 2 
                 10 
                 inf. 
               
             
          
           
               
                 Cycle 
                 1 
                 30 
                 1 
               
               
                   
               
             
          
         
       
     
     Merge Sequence (SEQ ID NO:8) Is 
     catcttcttcatcgtggtcaatgagttgt gaaagattttcctactatggaatgagagca ctcctgattctgtacttcagacgg ttcatcgggtgggacgataatctgtccacgg rcatctaccacacgtttgtggctctgt gctacctgacgccgatcctcggc gcactgatcgcagactcctggctgggaaag ttcaagacaatcgtgtcactctccattgtctacacaattggacaggcggtc actgcagtaagctcaattaatgacctc acagactataacaaagatggaactcc tgacaatctgtccgtgcatgtggcactgt ccatgattggcctggccctgatagctct gggaactggaggaataaagccctgtg tgtctgcatttgtggagaccagtttg aagagggccaggaaaaacaaagaaacag attcttttccatcttttantggccatt aatgctggaagcttgatttccactattg tcactcccatgctcagagttcacgaatgt ggaatttacagtcagaaagcttgtta cccactggcatttggggttcctgctgct ctcatggccgtatctctgattgtatttgtca ttggcagtggaatgtacaagaagtttcag ccccagggtaatgtcatgggtaa agttgtcaagtgcattggttttgccctc aaaaataggtttaggcaccggagt aagcagtttcccaagagggagcactggct ggactgggctaaagagaaatacgatgag cggctcatctctcaaattaagatggtcac aaaagtgatgttcttgtacatccc actcccaatgttctgggccctgtttgacc agcagggctccaggtggacactgcaagc aacagctatgagtgggaaaattg gacttcttgaagttcagccagatcagat gcagactgtgaatgccatcttgattgtc gtcatggtccccatcatggatgccgt ggtgtaccctctgattgcaaaatgtggc ttcaatttcacctccttgaagaggatg acagttggaatgttcctggcttccatgg ccttcgtgatggcggcgattgttcagct ggaaattgataaaactcttccagtctt ccccaaacaaaatgaagtccaaatcaa agtactgaatataggaaatggtgccatg aatgtatcttttcctggagcggtggtg acagttagccaaatgagtcaatcagat ggatttatgacttttgatgtagacaaac tgacaagtataaacatttcttccact ggatcaccagtcattccagtgacttataact ttgagcagggccatcgccatacccftctag tatgggcccccaataattaccgag tggtaaaggatggccttaaccagaa gccagaaaaaggagaaaatggaatcaga tttataaatagtcttaatgagag cctcaacatcaccatgggcgacaaagttt atgtgaatgtcaccagtcacaatgcc agcgagtatcagttcttttcttt gggcacaaaaaacattacaataagttcaacacaa cagatctcacaaaattgtacaaaagttct ccaatcatccaaccttgaatttgg tagtgcatatacctatgtaatcggaacgca gagcactggctgccctgaattgcatat gtttgaagatatttcacccaacacag ttaacatggctctgcagatcccgcagtac ttcctcatcacctgcggcgaggtggttttct ctgtcacaggactggagttctcatattct caggccccctccaacatgaagtc ggtgcttcaggcgggatggctgctgacagtggct  tgttggcaacatcattgtgtctcattgtggcaggagcaggccagtt   cagtgaaacagtgggctgaatacatcctatttgcggcattgcttct   ggttgtctgtgtaatatttgccatcatggcccggtttt   acacttacgtcaatccagcagagattg   
     
       
         
               
             
               
               
               
             
               
             
               
               
               
             
               
             
               
               
               
               
               
             
               
             
               
               
             
               
             
               
             
               
               
               
             
               
             
               
               
               
             
               
             
               
               
             
               
               
             
               
             
               
             
               
               
             
               
             
               
             
               
               
               
             
               
             
               
               
               
             
               
             
               
               
               
             
               
             
               
               
               
             
               
             
               
               
               
             
               
             
               
               
               
             
               
             
               
               
               
             
               
             
               
               
             
               
             
               
             
               
               
               
             
               
             
               
               
               
             
           
               
                 Multiple Alignment of Nucleotide Full Length Sequences 
               
             
          
           
               
                 Sequence 1: 
                 XM_007063Homosapiens 
                 3045 bp 
               
               
                   
               
               
                 Sequence 2: 
                 AY027496Ovis 
                 2829 bp 
               
               
                   
               
               
                 Sequence 3: 
                 D50306Rat 
                 2900 bp 
               
               
                   
               
               
                 Sequence 4: 
                 NM_053079Musmusculus 
                 3128 bp 
               
               
                   
               
               
                 Sequence 5: 
                 U13707Oryctolaguscunic 
                 2709 bp 
               
               
                   
               
               
                 Sequence 6: 
                 AY029615Gallusgallus 
                 2914 bp 
               
               
                   
               
               
                 Sequence 7: 
                 SequencetosubmitGenbak 
                 1840 bp 
               
               
                   
               
             
          
           
               
                 Start of Pairwise alignments 
               
               
                   
               
               
                 Aligning . . . 
               
               
                   
               
               
                 Sequences (4:5) Aligned. Score: 65 
               
               
                   
               
               
                 Sequences (1:2) Aligned. Score: 65 
               
               
                   
               
               
                 Sequences (2:3) Aligned. Score: 66 
               
               
                   
               
               
                 Sequences (3:4) Aligned. Score: 88 
               
               
                   
               
               
                 Sequences (4:6) Aligned. Score: 48 
               
               
                   
               
               
                 Sequences (2:4) Aligned. Score: 64 
               
               
                   
               
               
                 Sequences (1:3) Aligned. Score: 67 
               
               
                   
               
               
                 Sequences (3:5) Aligned. Score: 66 
               
               
                   
               
               
                 Sequences (4:7) Aligned. Score: 80 
               
               
                   
               
               
                 Sequences (2:5) Aligned. Score: 77 
               
               
                   
               
               
                 Sequences (3:6) Aligned. Score: 48 
               
               
                   
               
               
                 Sequences (5:6) Aligned. Score: 51 
               
               
                   
               
               
                 Sequences (1:4) Aligned. Score: 76 
               
               
                   
               
               
                 Sequences (3:7) Aligned. Score: 81 
               
               
                   
               
               
                 Sequences (5:7) Aligned. Score: 79 
               
               
                   
               
               
                 Sequences (2:6) Aligned. Score: 50 
               
               
                   
               
               
                 Sequences (6:7) Aligned. Score: 70 
               
               
                   
               
               
                 Sequences (1:5) Aligned. Score: 67 
               
               
                   
               
               
                 Sequences (2:7) Aligned. Score: 83 
               
               
                   
               
               
                 Sequences (1:6) Aligned. Score: 49 
               
               
                   
               
               
                 Sequences (1:7) Aligned. Score: 85 
               
               
                   
               
               
                 Guide tree        file created: 
               
               
                   
               
               
                 [/net/nfs0/vol1/production/w3nobody/tmp/999267.834538-239427.dnd] 
               
               
                   
               
               
                 Start of Multiple Alignment 
               
               
                   
               
               
                 There are 6 groups 
               
               
                   
               
               
                 Aligning . . . 
               
               
                   
               
             
          
           
               
                 Group 1: 
                 Sequences: 2 
                 Score: 48218 
               
               
                   
               
               
                 Group 2: 
                 Sequences: 3 
                 Score: 43200 
               
               
                   
               
               
                 Group 3: 
                 Sequences: 2 
                 Score: 42027 
               
               
                   
               
               
                 Group 4: 
                 Sequences: 5 
                 Score: 39817 
               
               
                   
               
               
                 Group 5: 
                 Sequences: 6 
                 Score: 30418 
               
               
                   
               
               
                 Group 6: 
                 Sequences: 7 
                 Score: 33857 
               
               
                   
               
             
          
           
               
                 Alignment Score 249395 
               
               
                   
               
               
                 CLUSTAL-Alignment file created 
               
               
                   
               
               
                 [/net/nfs0/vol1/production/w3nobody/tmp/999267.834538-239427.aln] 
               
               
                   
               
               
                 Your Multiple Sequence Alignment: 
               
               
                   
               
               
                 999267.834538-239427.aln 
               
               
                   
               
               
                 CLUSTAL W (1.81) multiple sequence alignment 
               
               
                   
               
             
          
           
               
                 D50306Rat 
                 -----------------------------------CTGAACTCCTGCTTG 
                 15 
                 (SEQ ID NO:3) 
                   
               
               
                   
               
               
                 NM_053079Musmusculus 
                 -------------------------------------------------- 
                   
                 (SEQ ID NO:4) 
               
               
                   
               
               
                 XM_007063Homosapiens 
                 -------------------------------------------------- 
                   
                 (SEQ ID NO:1) 
               
               
                   
               
               
                 AY027496Ovis 
                 -----GAAACAACATCTTTAGCACGGATTCCTCCCACCTGGACTCCTCGC 
                 45 
                 (SEQ ID NO:2) 
               
               
                   
               
               
                 U13707Oryctolaguscunic 
                 -------------------------------------------------- 
                   
                 (SEQ ID NO:5) 
               
               
                   
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
                   
                 (SEQ ID NO:7) 
               
               
                   
               
               
                 AY029615Gallusgallus 
                 GCTCTCTGTCCGTCCCTCGGTCCCTCCGTCCCTCCGTCCCCGCGCGGCCG 
                 50 
                 (SEQ ID NO:6) 
               
               
                   
               
               
                 D50306Rat 
                 CCAGTCGCCGGTCAGGAGCCTCGGAGCCGCCACAATGGGGATGTCCAAGT 
                 65 
               
               
                 NM_053079Musmusculus 
                 ---GTCGCCCGTCCGGAGCCTTGGAGCCACCACAATGGGGATGTCCAAGT 
                 47 
               
               
                 XM_007063Homosapiens 
                 --------------------------------------GAATGTCCAAAT 
                 12 
               
               
                 AY027496Ovis 
                 TCGCCAGTCGCAGGGAGCCCTCGGAGCCGCCAGCATGGGAATGTCCGTGC 
                 95 
               
               
                 U13707Oryctolaguscunic 
                 ------------------------------CACCATGGGAATGTCTAAGT 
                 20 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 CCAGCAGCGTGCCGGCCCCATGGCTGCAAAAAGTAAGAGTAAGGGCCGAT 
                 100 
               
               
                   
               
               
                 D50306Rat 
                 CT---CGGGGTTGCTTTGGCTACCCATTGAGCATCTTCTTCATCGTGGTC 
                 112 
               
               
                 NM_053079Musmusculus 
                 CT---CGGGGTTGCTTCGGTTACCCGTTGAGCATCTTCTTCATCGTGGTC 
                 94 
               
               
                 XM_007063Homosapiens 
                 CA---CACAGTTTCTTTGGTTATCCCCTGAGCATCTTCTTCATCGTGGTC 
                 59 
               
               
                 AY027496Ovis 
                 CG---AAGAGCTGCTTCGGTTACCCCTTAAGCATCTTCTTCATCGTGGTC 
                 142 
               
               
                 U13707Oryctolaguscunic 
                 CA---CTGAGCTGCTTCGGCTATCCCCTGAGCATCTTCTTCATCGTGGTC 
                 67 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------CATCTTCTTCATCGTGGTC 
                 19 
               
               
                 AY029615Gallusgallus 
                 CAGTGCCGAACTGCTTTGGCTACCCCTTGAGCATCTTCTTCATCGTCATC 
                 150 
               
               
                   
                                                ***************  ** 
               
               
                   
               
               
                 D50306Rat 
                 AATGAATTCTGTGAAAGATTCTCCTACTATGGGATGCGAGCTCTCCTGGT 
                 162 
               
               
                 NM_053079Musmusculus 
                 AATGAATTCTGTGAAAGATTCTCCTACTATGGCATGCGAGCACTCCTGGT 
                 144 
               
               
                 XM_007063Homosapiens 
                 AATGAGTTTTGCGAAAGATTTTCCTACTATGGAATGCGAGCAATCCTGAT 
                 109 
               
               
                 AY027496Ovis 
                 AATGAGTTCTGCGAAAGGTTCTCTTACTATGGAATGAGAGCACTCCTGAT 
                 192 
               
               
                 U13707Oryctolaguscunic 
                 AATGAGTTCTGCGAAAGGTTCTCCTACTATGGGATGAGAGCACTCCTGAT 
                 117 
               
               
                 SequencetosubmitGenbak 
                 AATGAGTTCTGTGAAAGATTTTCCTACTATGGAATGCGAGCAATCCTGAT 
                 69 
               
               
                 AY029615Gallusgallus 
                 AATGAGTTCTGCGAGAGGTTCTCCTACTATGGCATGCGAGCAATGCTCGT 
                 200 
               
               
                   
                 ***** ** ** ** ** ** ** ******** *** ****  * **  * 
               
               
                   
               
               
                 D50306Rat 
                 TCTGTACTTCAGGAACTTCCTTGGCTGGGATGATGACCTCTCCACGGCCA 
                 212 
               
               
                 NM_053079Musmusculus 
                 TCTGTACTTCAGGAACTTCCTCGGCTGGGACGACAATCTCTCCACGGCCA 
                 194 
               
               
                 XM_007063Homosapiens 
                 TCTGTACTTCACAAATTTCATCAGCTGGGATGATAACCTGTCCACCGCCA 
                 159 
               
               
                 AY027496Ovis 
                 CCTGTACTTCCAACGTTTCCTGGGCTGGAACGACAACCTGGGCACCGCCA 
                 242 
               
               
                 U13707Oryctolaguscunic 
                 TCTGTACTTCAGAAACTTCATCGGCTGGGACGACAACCTGTCCACGGTCA 
                 167 
               
               
                 SequencetosubmitGenbak 
                 TCTGTACTTCAGACGGTTCATCGGGTGGGACGATAATCTGTCCACGGCCA 
                 119 
               
               
                 AY029615Gallusgallus 
                 ATTGTATTTCAAGTACTTCCTGCGGTGGGATGACAACTTTTCTACAGCCA 
                 250 
               
               
                   
                   **** ***      *** *  * *** * **  *  *    ** * ** 
               
               
                   
               
               
                 D50306Rat 
                 TCTACCATACGTTTGTTGCCCTCTGCTACCTGACTCCAATTCTTGGAGCT 
                 262 
               
               
                 NM_053079Musmusculus 
                 TTTACCATACGTTCGTTGCCCTCTGCTACCTGACTCCAATTCTTGGAGCT 
                 244 
               
               
                 XM_007063Homosapiens 
                 TCTACCATACGTTTGTGGCTCTGTGCTACCTGACGCCAATTCTCGGAGCT 
                 209 
               
               
                 AY027496Ovis 
                 TCTATCACACGTTCGTCGCCCTGTGCTACCTGACGCCCATCCTCGGAGCT 
                 292 
               
               
                 U13707Oryctolaguscunic 
                 TCTACCACACGTTCGTCGCGCTGTGCTACCTCACGCCCATTCTCGGAGCT 
                 217 
               
               
                 SequencetosubmitGenbak 
                 TCTACCACACGTTTGTGGCTCTGTGCTACCTGACGCCGATCCTCGGCGCA 
                 169 
               
               
                 AY029615Gallusgallus 
                 TCTACCACACGTTTGTTGCTCTGTGCTACTTGACGCCCATCCTGGGAGCG 
                 300 
               
               
                   
                 * ** ** ***** ** ** ** ****** * ** ** ** ** ** ** 
               
               
                   
               
               
                 D50306Rat 
                 CTGATCGCAGACTCGTGGCTGGGGAAGTTCAAGACAATTGTCTCACTATC 
                 312 
               
               
                 NM_053079Musmusculus 
                 CTGATCGCAGACTCGTGGCTGGGGAAGTTCAAGACAATTGTTTCACTATC 
                 294 
               
               
                 XM_007063Homosapiens 
                 CTTATCGCCGACTCGTGGCTGGGAAAGTTCAAGACCATTGTGTCGCTCTC 
                 259 
               
               
                 AY027496Ovis 
                 CTCATCGCCGACTCCTGGCTGGGGAAGTTCAAGACGATCGTGTCGCTGTC 
                 342 
               
               
                 U13707Oryctolaguscunic 
                 CTCATCGCCGACGCGTGGCTGGGGAAGTTCAAGACCATCGTGTGGCTGTC 
                 267 
               
               
                 SequencetosubmitGenbak 
                 CTGATCGCAGACTCCTGGCTGGGAAAGTTCAAGACAATCGTGTCACTCTC 
                 219 
               
               
                 AY029615Gallusgallus 
                 CTCATTGCAGACTCATGGCTGGGAAAGTTTAAGACCATTGTCTCCCTGTC 
                 350 
               
               
                   
                 ** ** ** *** * ******** ***** ***** ** ** *  ** ** 
               
               
                   
               
               
                 D50306Rat 
                 CATCGTCTACACGATCGGACAGGCCGTCATCTCAGTGAGCTCAATTAATG 
                 362 
               
               
                 NM_053079Musmusculus 
                 CATCGTCTACACGATTGGACAAGCAGTCATCTCGGTGAGCTCAATTAATG 
                 344 
               
               
                 XM_007063Homosapiens 
                 CATTGTCTACACAATTGGACAAGCAGTCACCTCAGTAAGCTCCATTAATG 
                 309 
               
               
                 AY027496Ovis 
                 CATCGTCTACACCATTGGGCAGGTAGTCATCGCTGTGAGCTCAATTAATG 
                 392 
               
               
                 U13707Oryctolaguscunic 
                 CATCGTCTACACCATCGGACAAGCAGTCACCTCCCTCAGCTCCGTCAATG 
                 317 
               
               
                 SequencetosubmitGenbak 
                 CATTGTCTACACAATTGGACAGGCGGTCACTGCAGTAAGCTCAATTAATG 
                 269 
               
               
                 AY029615Gallusgallus 
                 CATTGTCTATACAATTGGGCAGGCAGTCATGGCTGTAAGCTCCATAAACG 
                 400 
               
               
                   
                 *** ***** ** ** ** ** *  ****   *  * *****  * ** * 
               
               
                   
               
               
                 D50306Rat 
                 ACCTTACAGACCATGACCACGACGGCAGTCCTAACAACCTTCCTTTGCAC 
                 412 
               
               
                 NM_053079Musmusculus 
                 ACCTCACAGACCACGACCACAATGGCAGTCCTGACAGCCTTCCCGTGCAC 
                 394 
               
               
                 XM_007063Homosapiens 
                 ACCTCACAGACCACAACCATGATGGCACCCCCGACAGCCTTCCTGTGCAC 
                 359 
               
               
                 AY027496Ovis 
                 ACCTCACTGACTTCAACCATGATGGAACCCCAAACAATATTTCTGTGCAC 
                 442 
               
               
                 U13707Oryctolaguscunic 
                 AGCTCACAGACAACAACCATGACGGGACCCCCGACAGCCTCCCTGTGCAC 
                 367 
               
               
                 SequencetosubmitGenbak 
                 ACCTCACAGACTATAACAAAGATGGAACTCCTGACAATCTGTCCGTGCAT 
                 319 
               
               
                 AY029615Gallusgallus 
                 ACATGACAGATCAAAACAGAGATGGCAATCCTGATAATATTGCGGTGCAC 
                 450 
               
               
                   
                 *  * ** **     **    * ** *  **  * *   *  *  **** 
               
               
                   
               
               
                 D50306Rat 
                 GTAGCACTGTCCATGATCGGCCTGGCCCTGATAGCCCTTGGTACAGGAGG 
                 462 
               
               
                 NM_053079Musmusculus 
                 GTAGCACTGTCCATGGTTGGCCTGGCCCTGATAGCCCTTGGTACAGGAGG 
                 444 
               
               
                 XM_007063Homosapiens 
                 GTGGTGCTGTCCTTGATCGGCCTGGCCCTGATAGCTCTCGGGACTGGAGG 
                 409 
               
               
                 AY0274696Ovis 
                 GTGGCACTCTCCATGATTGGCCTGGTCCTGATAGCTCTGGGTACCGGAGG 
                 492 
               
               
                 U13707Oryctolaguscunic 
                 GTGGCGGTGTGCATGATCGGCCTGCTCCTGATAGCCCTCGGGACAGGAGG 
                 417 
               
               
                 SequencetosubmitGenbak 
                 GTGGCACTGTCCATGATTGGCCTGGCCCTGATAGCTCTGGGAACTGGAGG 
                 369 
               
               
                 AY029615Gallusgallus 
                 ATTGCCCTGTCTATGACTGGCTTGATTCTCATCGCGCTTGGAACTGGTGG 
                 500 
               
               
                   
                  * *   * *   **   *** **   ** ** ** ** ** ** ** ** 
               
               
                   
               
               
                 D50306Rat 
                 AATCAAGCCCTGTGTGTCTGCATTTGGTGGCGATCAGTTTGAAGAGGGTC 
                 512 
               
               
                 NM_053079Musmusculus 
                 AATCAAGCCCTGTGTGTCTGCGTTTGGTGGCGATCAGTTTGAAGAGGGTC 
                 494 
               
               
                 XM_007063Homosapiens 
                 AATCAAACCCTGTGTGTCTGCGTTTGGTGGAGATCAGTTTGAAGAGGGCC 
                 459 
               
               
                 AY027496Ovis 
                 GATAAAGCCTTGCGTGTCTGCATTTGGCGGAGATCAGTTTGAAGAGGGCC 
                 542 
               
               
                 U13707Oryctolaguscunic 
                 AATCAAGCCCTGTGTGTCTGCCTTTGGCGGCGATCAGTTTGAGGAGGGCC 
                 467 
               
               
                 SequencetosubmitGenbak 
                 AATAAAGCCCTGTGTGTCTGCATTTGGTGGAGACCAGTTTGAAGAGGGCC 
                 419 
               
               
                 AY029615Gallusgallus 
                 GATCAAACCTTGTGTCTCAGCATTTGGTGGGGATCAGTTTGAAGAACATC 
                 550 
               
               
                   
                  ** ** ** ** ** ** ** ***** ** ** ******** **    * 
               
               
                   
               
               
                 D50306Rat 
                 AGGAAAAACAGCGAAACCGGTTCTTTTCCATCTTTTATTTGGCTATCAAC 
                 562 
               
               
                 NM_053079Musmusculus 
                 AGGAAAAACAGCGAAACCGGTTCTTTTCCATCTTTTATTTGGCTATCAAC 
                 544 
               
               
                 XM_007063Homosapiens 
                 AGGAGAAACAAAGAAACAGATTTTTTTCCATCTTTTACTTGGCTATTAAT 
                 509 
               
               
                 AY027496Ovis 
                 AGGAAAAGCAAAGGAACAGATTTTTTTCCATCTTTTATTTGGCCATTAAT 
                 592 
               
               
                 U13707Oryctolaguscunic 
                 AGGAAAAGCAAAGAAACCGGTTTTTTTCCATCTTTTACTTGGCCATTAAC 
                 517 
               
               
                 SequencetosubmitGenbak 
                 AGGAAAAACAAAGAAACAGATTCTTTTCCATCTTTTATTTGGCCATTAAT 
                 469 
               
               
                 AY029615Gallusgallus 
                 AGGAAAAACAAAGAAGTAGATTCTTCTCTATCTTTTATTTGTCCATTAAT 
                 600 
               
               
                   
                 **** ** **  * *   * ** ** ** ******** *** * ** ** 
               
               
                   
               
               
                 D50306Rat 
                 GCAGGAAGCCTGCTCTCCACGATCATCACTCCCATACTCAGAGTTCAGCA 
                 612 
               
               
                 NM_053079Musmusculus 
                 GGGGGAAGCCTGCTCTCCACGATCATCACTCCCATACTCAGAGTTCAACA 
                 594 
               
               
                 XM_007063Homosapiens 
                 GCTGGAAGTTTGCTTTCCACAATCATCACACCCATGCTCAGAGTTCAACA 
                 559 
               
               
                 AY027496Ovis 
                 GCTGGAAGTTTGCTTTCTACTATCATCACCCCCATGCTCAGAGTTCAGGT 
                 642 
               
               
                 U13707Oryctolaguscunic 
                 GCTGGGAGTCTGCTGTCCACAATCATCACCCCCATGGTCAGAGTTCAACA 
                 567 
               
               
                 SequencetosubmitGenbak 
                 GCTGGAAGCTTGATTTCCACTATTGTCACTCCCATGCTCAGAGTTCACGA 
                 519 
               
               
                 AY029615Gallusgallus 
                 GCTGGAAGTCTCATATCCACTATAATCACCCCAATTCTCAGAGCTCAAGA 
                 650 
               
               
                   
                 *  ** **  *  * ** ** **  **** ** **  ****** *** 
               
               
                   
               
               
                 D50306Rat 
                 GTGCGGAATCCACAGCCAACAAGCTTGTTACCCACTGGCCTTTGGGGTTC 
                 662 
               
               
                 NM_053079Musmusculus 
                 GTGCGGAATCCACAGTCAACAAGCTTGTTACCCACTGGCCTTCGGGGTTC 
                 644 
               
               
                 XM_007063Homosapiens 
                 ATGTGGAATTCACAGTAAACAAGCTTGTTACCCACTGGCCTTTGGGGTTC 
                 609 
               
               
                 AY027496Ovis 
                 ATGCGGAATTCACAGTAAGCAAGCTTGTTACCCCCTGGCCTTTGGGGTTC 
                 692 
               
               
                 U13707Oryctolaguscunic 
                 ATGTGGAATTCACGTTAAACAAGCTTGCTACCCACTGGCCTTTGGGATTC 
                 617 
               
               
                 SequencetosubmitGenbak 
                 ATGTGGAATTTACAGTCAGAAAGCTTGTTACCCACTGGCATTTGGGGTTC 
                 569 
               
               
                 AY029615Gallusgallus 
                 ATGTGGCATTCACAGCAGACAGCAGTGCTACCCGCTGGCATTTGGAGTTC 
                 700 
               
               
                   
                  ** ** **  **       *    ** ***** ***** ** **  *** 
               
               
                   
               
               
                 D50306Rat 
                 CGGCAGCTCTCATGGCTGTTGCCCTAATTGTGTTTGTCCTCGGCAGTGGA 
                 712 
               
               
                 NM_053079Musmusculus 
                 CAGCGGCTCTCATGGCTGTTGCCCTAATTGTGTTTGTCCTTGGCAGTGGA 
                 694 
               
               
                 XM_007063Homosapiens 
                 CTGCTGCTCTCATGGCTGTAGCCCTGATTGTGTTTGTCCTTGGCAGTGGG 
                 659 
               
               
                 AY027496Ovis 
                 CTGCTGCACTCATGGCTGTATCTCTGATCGTGTTTGTCATTGGCAGTGGA 
                 742 
               
               
                 U13707Oryctolaguscunic 
                 CTGCTATCCTCATGGCTGTATCCCTGATCGTGTTCATCATCGGCAGTGGG 
                 667 
               
               
                 SequencetosubmitGenbak 
                 CTGCTGCTCTCATGGCCGTATCTCTGATTGTATTTGTCATTGGCAGTGGA 
                 619 
               
               
                 AY029615Gallusgallus 
                 CCGCTGCCCTCATGGCTGTTTCATTAGTTGTGTTCATAGCTGGAAGTGGA 
                 750 
               
               
                   
                 * **    ******** **  *  *  * ** **  *    ** ***** 
               
               
                   
               
               
                 D50306Rat 
                 ATGTACAAGAAGTTTCAGCCCCAGGGCAACATCATGGGCAAAGTGGCCAA 
                 762 
               
               
                 NM_053079Musmusculus 
                 ATGTACAAGAAGTTCCAGCCCCAGGGCAACATCATGGGCAAAGTGGCCAA 
                 744 
               
               
                 XM_007063Homosapiens 
                 ATGTACAAGAAGTTCAAGCCACAGGGCAACATCATGGGTAAAGTGGCCAA 
                 709 
               
               
                 AY027496Ovis 
                 ATGTACAAGAAGGTCCAGCCCCAGGGTAACATCATGTCTAAAGTTGCCAG 
                 792 
               
               
                 U13707Oryctolaguscunic 
                 ATGTACAAGAAGTTCAAGCCGCAGGGGAACATCCTGAGCAAAGTGGTGAA 
                 717 
               
               
                 SequencetosubmitGenbak 
                 ATGTACAAGAAGTTTCAGCCCCAGGGTAATGTCATGGGTAAAGTTGTCAA 
                 669 
               
               
                 AY029615Gallusgallus 
                 ATGTACAAAAAAGTTCAACCGCAAGGCAATATAATGGTTCGAGTTTGTAA 
                 800 
               
               
                   
                 ******** **  *  * ** ** ** **  *  **     ***    * 
               
               
                   
               
               
                 D50306Rat 
                 GTGCATTGGCTTTGCCATCAAAAACAGGTTTCGGCACCGAAGTAAGGCAT 
                 812 
               
               
                 NM_053079Musmusculus 
                 GTGCATTGGTTTTGCCATCAAAAACAGGTTTCGGCACCGAAGTAAGGCAT 
                 794 
               
               
                 XM_007063Homosapiens 
                 GTGCATCGGTTTTGCCATCAAAAATAGATTTAGGCATCGGAGTAAGGCAT 
                 759 
               
               
                 AY027496Ovis 
                 GTGCATTGGGTTTGCCATCAAAAATAGGATTAGCCATCGGAGTAAGAAAT 
                 842 
               
               
                 U13707Oryctolaguscunic 
                 GTGCATCTGCTTTGCCATCAAAAATAGGTTTAGGCACCGCAGTAAGCAGT 
                 767 
               
               
                 SequencetosubmitGenbak 
                 GTGCATTGGTTTTGCCCTCAAAAATAGGTTTAGGCACCGGAGTAAGCAGT 
                 719 
               
               
                 AY029615Gallusgallus 
                 ATGCATTGGATTTGCCATTAAAAACAGGTTTCGGCATCGCAGCAAAGAGT 
                 850 
               
               
                   
                  *****  * ****** * ***** **  ** * ** ** ** **    * 
               
               
                   
               
               
                 D50306Rat 
                 TTCCCAAGAGGGAACACTGGCTGGACTGGGCTAAAGAGAAATACGATGAG 
                 862 
               
               
                 NM_053079Musmusculus 
                 ATCCCAAGAGGGAGCACTGGCTGGACTGGGCTAAAGAGAAATACGACGAG 
                 844 
               
               
                 XM_007063Homosapiens 
                 TTCCCAAGAGGGAGCACTGGCTGGACTGGGCTAAAGAGAAATACGATGAG 
                 809 
               
               
                 AY027496Ovis 
                 TTCCTAAGAGGGAGCACTGGCTGGACTGGGCTAGCGAGAAATATGATGAG 
                 892 
               
               
                 U13707Oryctolaguscunic 
                 TTCCCAAGAGGGCGCACTGGCTGGACTGGGCTAAGGAGAAATACGACGAG 
                 817 
               
               
                 SequencetosubmitGenbak 
                 TTCCCAAGAGGGAGCACTGGCTGGACTGGGCTAAAGAGAAATACGATGAG 
                 769 
               
               
                 AY029615Gallusgallus 
                 ATCCCAAAAGAGAGCACTGGCTAGACTGGGCAAGCGAGAAGTATGATAAA 
                 900 
               
               
                   
                  *** ** ** *  ******** ******** *  ***** ** **  * 
               
               
                   
               
               
                 D50306Rat 
                 AGGCTCATCTCGCAGATTAAGATGGTGACGAAGGTGATGTTCCTGTACAT 
                 912 
               
               
                 NM_053079Musmusculus 
                 CGGCTCATCTCACAGATTAAGATGGTCACGAAGGTGATGTTCCTGTTCAT 
                 894 
               
               
                 XM_007063Homosapiens 
                 CGGCTCATCTCCCAAATTAAGATGGTTACGAGGGTGATGTTCCTGTATAT 
                 859 
               
               
                 AY027496Ovis 
                 CGGCTCATCTCTCAAATTAAGATGGTTACAAGGGTGATGTTCCTGTACAT 
                 942 
               
               
                 U13707Oryctolaguscunic 
                 CGGCTTATCGCGCAGATCAAGATGGTTACGAGGGTGCTGTTCCTGTACAT 
                 867 
               
               
                 SequencetosubmitGenbak 
                 CGGCTCATCTCTCAAATTAAGATGGTCACAAAAGTGATGTTCTTGTACAT 
                 819 
               
               
                 AY029615Gallusgallus 
                 CGACTGATTGCTCAGACCAAGATGGTGTTGAAGGTGCTTTTCCTTTACAT 
                 950 
               
               
                   
                  * ** **  * ** *  ********    *  *** * *** * *  ** 
               
               
                   
               
               
                 D50306Rat 
                 TCCCCTCCCCATGTTTTGGGCCTTGTTTGACCAGCAGGGTTCCAGGTGGA 
                 962 
               
               
                 NM_053079Musmusculus 
                 CCCACTCCCCATGTTCTGGGGCCTGTTTGACCAACAAGGGTCCAGATGGA 
                 944 
               
               
                 XM_007063Homosapiens 
                 TCCACTCCCAATGTTCTGGGCCTTGTTTGACCAGCAGGGCTCCAGGTGGA 
                 909 
               
               
                 AY027496Ovis 
                 TCCTCTCCCCATGTTCTGGGCCTTGTTTGATCAGCAGGGCTCCAGGTGGA 
                 992 
               
               
                 U13707Oryctolaguscunic 
                 CCCACTCCCCATGTTCTGGGCCTTGTTTGATCAGCAGGGTTCCAGATGGA 
                 917 
               
               
                 SequencetosubmitGenbak 
                 CCCACTCCCAATGTTCTGGGCCCTGTTTGACCAGCAGGGCTCCAGGTGGA 
                 869 
               
               
                 AY029615Gallusgallus 
                 CCCTCTCCCGATGTTCTGGGCACTTTTTGACCAGCAGGGATCGAGATGGA 
                 1000 
               
               
                   
                  ** ***** ***** ****   * ***** ** ** ** ** ** **** 
               
               
                   
               
               
                 D50306Rat 
                 CACTGCAAGCAACGACCATGACTGGGAAAATTGGAACAATTGAGATTCAG 
                 1012 
               
               
                 NM_053079Musmusculus 
                 CACTGCAAGCAACGACCATGAATGGGAAAATTGGAGCAAATGAAATTCAG 
                 994 
               
               
                 XM_007063Homosapiens 
                 CACTGCAGGCAACAACTATGTCCGGGAAAATCGGAGCTCTTGAAATTCAG 
                 959 
               
               
                 AY027496Ovis 
                 CACTGCAAGCAACGACCATGAGTGGGAAGATTGGAATCATTGAAATCCAG 
                 1042 
               
               
                 U13707Oryctolaguscunic 
                 CGCTGCAAGCGACGACCATGTCCGGGAGAATTGGAATCCTTGAAATTCAG 
                 967 
               
               
                 SequencetosubmitGenbak 
                 CACTGCAAGCAACAGCTATGAGTGGGAAAATTGGACTTCTTGAAGTTCAG 
                 919 
               
               
                 AY029615Gallusgallus 
                 CACTGCAAGCCACAACTATGGATGGGGACTTTGGAGCTATGCAGATTCAG 
                 1050 
               
               
                   
                 * ***** ** **  * ***   ***    * ***       *  * *** 
               
               
                   
               
               
                 D50306Rat 
                 CCGGACCAGATGCAGACGGTGAACGCCATCTTGATTGTCATCATGGTCCC 
                 1062 
               
               
                 NM_053079Musmusculus 
                 CCGGACCAGATGCAGACGGTGAATGCCATCCTGAATGTCAACAATGGCCC 
                 1044 
               
               
                 XM — 007063Homosapiens 
                 CCCGATCAGATGCAGACCGTGAACGCCATCCTGATCGTGATCATGGTCCC 
                 1009 
               
               
                 AY027496Ovis 
                 CCGGATCAGATGCAGACGGTGAACGCCATCCTGATCGTCGTCATGGTCCC 
                 1092 
               
               
                 U13707Oryctolaguscunic 
                 CCGGATCAGATGCAGACTGTGAACACCATCTTGATTATTATCCTGGTCCC 
                 1017 
               
               
                 SequencetosubmitGenbak 
                 CCAGATCAGATGCAGACTGTGAATGCCATCTTGATTGTCGTCATGGTCCC 
                 969 
               
               
                 AY029615Gallusgallus 
                 CCAGACCAAATGCAGACTGTCAATCCAATCCTGATTATAATAATGGTCCC 
                 1100 
               
               
                   
                 ** ** ** ******** ** **  * *** ***   *       * *** 
               
               
                   
               
               
                 D50306Rat 
                 CATTGTGGACGCCGTGGTGTATCCGCTCATTGCAAAATGTGGTTTCAACT 
                 1112 
               
               
                 NM_053079Musmusculus 
                 CAATGTGGACGCCGTTGTGTACCGCTCAATTGCAAAATGTGGTTTCAACT 
                 1094 
               
               
                 XM_007063Homosapiens 
                 GATCTTCGATGCTGTGCTGTACCCTCTCATTGCAAAATGTGGCTTCAATT 
                 1059 
               
               
                 AY027496Ovis 
                 CATCGTGGATGCCGTGGTATATCCTCTGATCGCAAAGTGTGGTTTAAATT 
                 1142 
               
               
                 U13707Oryctolaguscunic 
                 CATCATGGACGCCGTGGTGTATCCTCTGATTGCAAAGTGTGGCCTCAACT 
                 1067 
               
               
                 SequencetosubmitGenbak 
                 CATCATGGATGCCGTGGTGTACCCTCTGATTGCAAAATGTGGCTTCAATT 
                 1019 
               
               
                 AY029615Gallusgallus 
                 AGTTGTAGATGCTGTGATTTATCCTTTAATCCAGAAATGCAAGATCAATT 
                 1150 
               
               
                   
                      * ** ** **  * ** *     **    ** **     * ** * 
               
               
                   
               
               
                 D50306Rat 
                 TCACCTCCCTGAAGAAGATGACCGTTGGGATGTTCCTGGCATCCATGGCC 
                 1162 
               
               
                 NM_053079Musmusculus 
                 TCACATCCCTGAAGAAGATGACTGTTGGGATGTTCCTGGCGTCCATGGCC 
                 1144 
               
               
                 XM_007063Homosapiens 
                 TCACCTCCTTGAAGAAGATGGCAGTTGGCATGGTCCTGGCCTCCATGGCC 
                 1109 
               
               
                 AY027496Ovis 
                 TCACCTCCCTGAAGAAGATGACCGTCGGCATGTTTCTGGCCTCCATGGCT 
                 1192 
               
               
                 U13707Oryctolaguscunic 
                 TCACCTCTCTGAAGAAGATGACGATTGGGATGTTCCTGGCTTCCATGGCC 
                 1117 
               
               
                 SequencetosubmitGenbak 
                 TCACCTCCTTGAAGAGGATGACAGTTGGAATGTTCCTGGCTTCCATGGCC 
                 1069 
               
               
                 AY029615Gallusgallus 
                 TTACGCCCCTGAGGAGGATCACTGTTGGCATGTTCCTTGCTGGTCTGGCT 
                 1200 
               
               
                   
                 * **  *  *** ** ***  *  * ** *** * ** **     **** 
               
               
                   
               
               
                 D50306Rat 
                 TTTGTGGTGGCTGCAATTGTGCAGGTGGAAATCGATAAAACTCTTCCAGT 
                 1212 
               
               
                 NM_053079Musmusculus 
                 TTTGTGGTGGCTGCAATTGTGCAGGTGGAAATCGATAAAACTCTTCCAGT 
                 1194 
               
               
                 XM_007063Homosapiens 
                 TTTGTGGTGGCTGCCATCGTGCAGGTGGAAATCGATAAAACTCTTCCAGT 
                 1159 
               
               
                 AY027496Ovis 
                 TTCGTGGCAGCTGCCATCGTGCAGGTGGACATTGACAAAACTCTGCCCGT 
                 1242 
               
               
                 U13707Oryctolaguscunic 
                 TTCGTGGCAGCTGCAATCCTGCAGGTGGAAATCGATAAAACTCTTCCTGT 
                 1167 
               
               
                 SequencetosubmitGenbak 
                 TTCGTGATGGCGGCGATTGTTCAGCTGGAAATTGATAAAACTCTTCCAGT 
                 1119 
               
               
                 AY029615Gallusgallus 
                 TTCGTTGCTGCTGCTCTTTTGCAAGTGCAAATAGATAAAACTCTTCCAGT 
                 1250 
               
               
                   
                 ** **    ** **  *  * **  ** * ** ** ******** ** ** 
               
               
                   
               
               
                 D50306Rat 
                 CTTCCCCAGCGGAAATCAAGTTCAAATTAAGGTCTTGAACATTGGAAACA 
                 1262 
               
               
                 NM_053079Musmusculus 
                 CTTCCCTGGTGGAAATCAAGTCCAAATTAAGGTCTTGAACATCGGAAACA 
                 1244 
               
               
                 XM_007063Homosapiens 
                 CTTCCCCAAAGGAAACGAAGTCCAAATTAAAGTTTTGAATATAGGAAACA 
                 1209 
               
               
                 AY027496Ovis 
                 CTTCCCCAAAGGAAATGAAGTCCAAATCAAAGTCCTGAATATAGGAAATA 
                 1292 
               
               
                 U13707Oryctolaguscunic 
                 CTTCCCCAAAGCCAATGAAGTCCAAATTAAAGTTCTGAATGTAGGAAGTG 
                 1217 
               
               
                 SequencetosubmitGenbak 
                 CTTCCCCAAACAAAATGAAGTCCAAATCAAAGTACTGAATATAGGAAATG 
                 1169 
               
               
                 AY029615Gallusgallus 
                 TTTCCCTGCAGCTGGACAGGCCCAAATCAAAATAATAAATCTAGGTGATA 
                 1300 
               
               
                   
                  *****           * *  ***** **  *  * **  * ** 
               
               
                   
               
               
                 D50306Rat 
                 ATGACATGGCCGTGTATTTTCCTGGAAAGAATGTGACAGTTGCCCAAATG 
                 1312 
               
               
                 NM_053079Musmusculus 
                 ATAACATGACCGTGCATTTTCCTGGAAATAGTGTGACGCTTGCCCAAATG 
                 1294 
               
               
                 XM_007063Homosapiens 
                 ATACCATGAATATATCTCTTCCTGGAGAGATGGTGACACTTGGCCCAATG 
                 1259 
               
               
                 AY027496Ovis 
                 ATAGCATGACCGTGTCTTTTCCCGGAACGACAGTGACATGTGACCAGATG 
                 1342 
               
               
                 U13707Oryctolaguscunic 
                 AGAACATGATCATCTCTCTTCCTGGGCAGACGGTGACGCTCAACCAGATG 
                 1267 
               
               
                 SequencetosubmitGenbak 
                 GTGCCATGAATGTATCTTTTCCTGGAGCGGTGGTGACAGTTAGCCAAATG 
                 1219 
               
               
                 AY029615Gallusgallus 
                 GCAATGCGAATGT-TACATTTCTGCCTAATCTTCAGAACGTGACTGTCCT 
                 1349 
               
               
                   
                        *    *     ** * *                   * 
               
               
                   
               
               
                 D50306Rat 
                 TCTCA---GACAGACACATT-CATGACTTTCGATGTAGACCAGCTGACAA 
                 1358 
               
               
                 NM_053079Musmusculus 
                 TCTCA---GACAGACACGTT-CATGACTTTCGATATAGACAAGCTGACAA 
                 1340 
               
               
                 XM_007063Homosapiens 
                 TCTCA---AACAAATGCATT-TATGACTTTTGATGTAAACAAACTGACAA 
                 1305 
               
               
                 AY027496Ovis 
                 TCTCA---AACAAACGGATT-TCTGACTTTCAACGTAGACAACCT---AA 
                 1385 
               
               
                 U13797Oryctolaguscunic 
                 TCTCA---AACGAATGAATT-CATGACTTTCAATGAAGACACACTGACAA 
                 1313 
               
               
                 SequencetosubmitGenbak 
                 AGTCA---ATCAGATGGATT-TATGACTTTTGATGTAGACAAACTGACAA 
                 1265 
               
               
                 AY029615Gallusgallus 
                 TCCCATGGAGTCAACAGGCTACAGGATGTTTGAGTCTTCCCAGCTAAAAT 
                 1399 
               
               
                   
                    **        *     *    **  **  *      *   **   * 
               
               
                   
               
               
                 D50306Rat 
                 GCATAAACGTGTCTTCTCCCGG-ATCTCCAGGCGTCACCACGGTAGCTCA 
                 1407 
               
               
                 NM_053079Musmusculus 
                 GCATAAACATATCTTCCTCTGG-ATCCCCAGGAGTCACCACAGTAGCTCA 
                 1389 
               
               
                 XM_007063Homosapiens 
                 GGATAAACATTTCTTCTCCTGG-ATCACCAG---TCACTGCTGTAACTGA 
                 1351 
               
               
                 AY027496Ovis 
                 GTATAAACATTTCTTCTACTGG-AACACCAG---TCACTCCAGTAACTCA 
                 1431 
               
               
                 U13707Oryctolaguscunic 
                 GCATAAACATCACTTCC---GG-ATCACAAG---TCACCATGATCACACC 
                 1356 
               
               
                 SequencetosubmitGenbak 
                 GTATAAACATTTCTTCCACTGG-ATCACCAG---TCATTCCAGTGACTTA 
                 1311 
               
               
                 AY029615Gallusgallus 
                 CTGTAATGGTAAATTTTGGGAGTGAGAGTAGAAGTGAAAATATCGACTCA 
                 1449 
               
               
                   
                    ***   *   **      *       **   * *         * 
               
               
                   
               
               
                 D50306Rat 
                 -TGAGTTTGAGCCGGGTCACCGGCACACCCTTCTAGTGTGGGGCCCCAAT 
                 1456 
               
               
                 NM_053079Musmusculus 
                 -TGATTTTGAGCAGGGTCACCGGCACAACCTTCTAGTGTGGGAACCCAGT 
                 1438 
               
               
                 XM_007063Homosapiens 
                 -CGACTTCAAGCAGGGCCAACGCCACACGCTTCTAGTGTGGGCCCCCAAT 
                 1400 
               
               
                 AY027496Ovis 
                 -TAACTTTGAGTCCGGCCATCGCCATACCCTTCTCGTCTGGGCCCCAAGT 
                 1480 
               
               
                 U13707Oryctolaguscunic 
                 -CAGCCTTGAGGCAGGCCAGCGCCACACCCTGCTGGTGTGGGCCCCCAAT 
                 1405 
               
               
                 SequencetosubmitGenbak 
                 -TAACTTTGAGCAGGGCCATCGCCATACCCTTCTAGTATGGGCCCCCAAT 
                 1360 
               
               
                 AY029615Gallusgallus 
                 ATAAGCAGCAATACGCATACTGTCACCATCAAGAATGCAGCAGCCGGCAT 
                 1499 
               
               
                   
                          *    *   *  * **    *         *    *    * 
               
               
                   
               
               
                 D50306Rat 
                 CTATACCGTGTGGTAAA-AGACGGTCTTAACCAAAAGCCAGAGAAAGGGG 
                 1505 
               
               
                 NM_053079Musmusculus 
                 CAATACCGTGTGGTAAA-AGATGGTCCTAACCAAAAGCCAGAGAAAGGGG 
                 1487 
               
               
                 XM_007063Homosapiens 
                 CACTACCAGGTGGTAAA-GGATGGTCTTAACCAGAAGCCAGAAAAAGGGG 
                 1449 
               
               
                 AY027496Ovis 
                 AACTACCAAGTGGTAAA-AGATGGCCTTAACCAGAAGCCAGAAAAAGGGA 
                 1529 
               
               
                 U13707Oryctolaguscunic 
                 AACTACCGAGTGGTCAA-TGACGGCCTGACCCAGAAGTCAGACAAAGGAG 
                 1454 
               
               
                 SequencetosubmitGenbak 
                 AATTACCGAGTGGTAAA-GGATGGCCTTAACCAGAAGCCAGAAAAAGGAG 
                 1409 
               
               
                 AY029615Gallusgallus 
                 TGTTTCTAGCTTGCGGTCTGATAATTTCACATCAAAACCAGAAGAAGGAA 
                 1549 
               
               
                   
                    * *    * *      **       *     **  ****  **** 
               
               
                   
               
               
                 D50306Rat 
                 AGAACGGAATCAGATTCGTCAGCACCCTTAACGAGATGATCACCATCAAA 
                 1555 
               
               
                 NM_053079Musmusculus 
                 AGAACGGAATCAGGTTTGTCAACACCCTTAACGAGATGGTCACCAACAAA 
                 1537 
               
               
                 XM_007063Homosapiens 
                 AAAATGGAATCAGATTTGTAAATACTTTTAACGAGCTCATCACCATCACA 
                 1499 
               
               
                 AY027496Ovis 
                 GAAATGGAATCAGATTCGTTAATGCTTTTGGCGAGAGCTTCGGCGTCACA 
                 1579 
               
               
                 U13707Oryctolaguscunic 
                 AAAATGGAATCAGGTTTGTGAACACTTACAGCCAGCCCATCAACGTCACG 
                 1504 
               
               
                 SequencetosubmitGenbak 
                 AAAATGGAATCAGATTTATAAATAGTCTTAATGAGAGCCTCAACATCACC 
                 1459 
               
               
                 AY029615Gallusgallus 
                 AGAATCTAGTCAGGTTTGTAAATAATTTGCCTCAGACAGTCAACATCACT 
                 1599 
               
               
                   
                   **   * **** **  * *            **    **  *  ** 
               
               
                   
               
               
                 D50306Rat 
                 ATGAGTGGAAAAGTGTACGAAAATGTCACCAGTCACAG-CGCCAGCAACT 
                 1604 
               
               
                 NM_053079Musmusculus 
                 ATGAGTGGGAAAGTATATGAAAAATTCACAAGTCACAA-CGCCAGCGGCT 
                 1586 
               
               
                 XM_007063Homosapiens 
                 ATGAGTGGGAAAGTTTATGCAAACATCAGCAGCTACAA-TGCCAGCACAT 
                 1548 
               
               
                 AY027496Ovis 
                 ATGGATGGGGAAGTTTACAACAATGTCTCCGGTCACAA-TGCCAGTGAAT 
                 1628 
               
               
                 U13707Oryctolaguscunic 
                 ATGAGCGGGAAAGTTTACGAACACATCGCCAGCTACAA-TGCCAGCGAGT 
                 1553 
               
               
                 SequencetosubmitGenbak 
                 ATGGGCGACAAAGTTTATGTGAATGTCACCAGTCACAA-TGCCAGCGAGT 
                 1508 
               
               
                 AY029G15Gallusgallus 
                 ATGGGTGACACGACTTTTG-GAATACTGGAAGAGACAAGTATCAGTAATT 
                 1648 
               
               
                   
                 ***   *        *      *        *  ***     ***    * 
               
               
                   
               
               
                 D50306Rat 
                 ATCAGTTTTTCCCTTCTGGCCAAAAAGACTACACAATAAACACCACAGA- 
                 1653 
               
               
                 NM_053079Musmusculus 
                 ACAAGTTCCTCCCTTCTGGCGAAAAGCAGTACACAATAAACACCACGGC- 
                 1635 
               
               
                 XM_007063Homosapiens 
                 ACCAGTTTTTTCCTTCTGGCATAAAAGGCTTCACAATAAGCTCAACAGA- 
                 1597 
               
               
                 AY027496Ovis 
                 ATCTTTTTTTCTCTTCTGGCGTAAAGAGCTTCACAATAAACTCACCAGA- 
                 1677 
               
               
                 U13707Oryctolaguscunic 
                 ATCAGTTTTTCACTTCTGGAGTAAAGGGCTTCACCGTCAGCTCGGCAGG- 
                 1602 
               
               
                 SequencetosubmitGenbak 
                 ATCAGTTCTTTTCTTTGGGCACAAAAAACATTACAATAAGTTCAACACAA 
                 1558 
               
               
                 AY029615Gallusgallus 
                 ACAGTCCGTTCTCAGGAGGAAGAACATATGATATAGTGATAACTGCAGG- 
                 1697 
               
               
                   
                 *        *  *    **   **        *   * *   *  * 
               
               
                   
               
               
                 D50306Rat 
                 --GATTGCACCAAACTGTTCATCTGATTTTAAATCTTCCAACCTTGACTT 
                 1701 
               
               
                 NM_053079Musmusculus 
                 --GGTGGCACCAACCTGTCTAACTGATTTTAAATCTTCCAACCTTGACTT 
                 1683 
               
               
                 XM_007063Homosapiens 
                 --GATTCCGCCACAATGTCAACCTAATTTCAATACTTTCTACCTTGAATT 
                 1645 
               
               
                 AY027496Ovis 
                 --GATTTCACAACAGTGTGAAAAACAGTTCAAAACATCCTACCTTGAATT 
                 1725 
               
               
                 U13707Oryctolaguscunic 
                 --CATCTCGGAGCAGTGCAGGCGGGACTTTGAGTCTCCGTACCTGGAGTT 
                 1650 
               
               
                 SequencetosubmitGenbak 
                 CAGATCTCACAAAATTGTACAAAAGTTCTCCAATCATCCAACCTTGAATT 
                 1608 
               
               
                 AY029615Gallusgallus 
                 -----TTCAACTAATTGCAAACC-AACTTCAGAG-----AAATTAGGATA 
                 1736 
               
               
                   
                        *       **           *           *  * *  * 
               
               
                   
               
               
                 D50306Rat 
                 CGGCAGCGCGTACACCTACGTGATCAGAAGTAGGGCGAGTGATGGCTGCC 
                 1751 
               
               
                 NM_053079Musmusculus 
                 TGGCAGCGCGTATACCTACGTGATCCGA---AGGGCGAGTGATGGCTGCC 
                 1730 
               
               
                 XM_007063Homosapiens 
                 TGGTAGTGCTTATACCTATATAGTCCAA---AGGAAGAATGACAGCTGCC 
                 1692 
               
               
                 AY027496Ovis 
                 TGGTAGTGCGTTTACCTATGTAATCAGC---AGAAAGAGTGACGGTTGCC 
                 1772 
               
               
                 U13707Oryctolaguscunic 
                 TGGCAGCGCGTACACGTACCTGATCACG---AGCCAGGCTACTGGCTGCC 
                 1697 
               
               
                 SequencetosubmitGenbak 
                 TGGTAGTGCATATACCTATGTAATCGGA---ACGCAGAGCACTGGCTGCC 
                 1655 
               
               
                 AY029615Gallusgallus 
                 TGGTGGTGCTTATACGATCGTAATTAAT---GAGTGTTCTGGAGATGTGA 
                 1783 
               
               
                   
                  **  * ** *  **     *  * 
               
               
                   
               
               
                 D50306Rat 
                 TGGAAGTGAAGGAATTCGAAGACATCCCACCCAACACGGTGAACATGGCC 
                 1801 
               
               
                 NM_053079Musmusculus 
                 TGGAAGTGAAGGAATTTGAAGACATCCCACCCAACACTGTGAACATGGCT 
                 1780 
               
               
                 XM_007063Homosapiens 
                 CTGAAGTGAAGGTGTTTGAAGATATTTCAGCCAACACAGTTAACATGGCT 
                 1742 
               
               
                 AY027496Ovis 
                 CCGAACCAAAGATTTTCGAAGACATCTCCCCCAACACAGTCAGCATGGCT 
                 1822 
               
               
                 U13707Oryctolaguscunic 
                 CCCAAGTGACGGAGTTTGAAGATATTCCGCCCAACACAATGAACATGGCT 
                 1747 
               
               
                 SequencetosubmitGenbak 
                 CTGAATTGCATATGTTTGAAGATATTTCACCCAACACAGTTAACATGGCT 
                 1705 
               
               
                 AY029615Gallusgallus 
                 CTCAATTAAGATACATTGAAGATATCCAACCCAATACAGTCCATATGGCT 
                 1833 
               
               
                   
                    **          * ***** **     **** **  *    ***** 
               
               
                   
               
               
                 D50306Rat 
                 CTGCAGATCCCACAGTACTTCCTCCTCACCTGCGGCGAGGTGGTCTTCTC 
                 1851 
               
               
                 NM_053079Musmusculus 
                 CTGCAGATCCCACAGTACTTCCTTCTCACCTGCGGCGAGGTGGTCTTCTC 
                 1830 
               
               
                 XM_007063Homosapiens 
                 CTGCAAATCCCGCAGTATTTTCTTCTCACCTGTGGCGAAGTGGTCTTCTC 
                 1792 
               
               
                 AY027496Ovis 
                 CTGCAGATCCCCCAGTACTTCCTCCTCACCTGTGGCGAGGTGGTCTTCTC 
                 1872 
               
               
                 U13707Oryctolaguscunic 
                 TGGCAAATCCCACAGTACTTCCTCATCACCTCTGGCGAGGTGGTCTTCTC 
                 1797 
               
               
                 SequencetosubmitGenbak 
                 CTGCAGATCCCGCAGTACTTCCTCATCACCTGCGGCGAGGTGGTTTTCTC 
                 1755 
               
               
                 AY029615Gallusgallus 
                 TGGCAGATCCCTCAGTATTTCATACTTACATGTGGAGAAGTAGTCTTCTC 
                 1883 
               
               
                   
                   *** ***** ***** **  *  * ** *  ** ** ** ** ***** 
               
               
                   
               
               
                 D50306Rat 
                 TGTCACAGGACTGGAGTTCTCCTATTCCCAGGCCCCGTCTAACATGAAGT 
                 1901 
               
               
                 NM_053079Musmusculus 
                 TGTCACAGGACTGGAGTTCTCTTATTCCCAGGCTCCGTCTAACATGAAGT 
                 1880 
               
               
                 XM_007063Homosapiens 
                 TGTCACGGGATTGGAATTCTCATATTCTCAGGCTCCTTCCAACATGAAGT 
                 1842 
               
               
                 AY027496Ovis 
                 CATCACCGGCCTGGAGTTCTCCTATTCTCAGGCTCCTTCCAACATGAAGT 
                 1922 
               
               
                 U13707Oryctolaguscunic 
                 CATCACGGGCCTGGAGTTCTCCTATTCTCAGGCTCCTTCCAACATGAAGT 
                 1847 
               
               
                 SequencetosubmitGenbak 
                 TGTCACAGGACTGGAGTTCTCATATTCTCAGGCCCCCTCCAACATGAAGT 
                 1805 
               
               
                 AY029615Gallusgallus 
                 TGTCACTGGGCTGGAGTTTTCATACTCACAGGCACCATCTAATATGAAGT 
                 1933 
               
               
                   
                   **** **  **** ** ** ** ** ***** ** ** ** ******* 
               
               
                   
               
               
                 D50306Rat 
                 CCGTGCTTCAGGCAGgATGGCTTCTAACCGTGGCCATCGGTAATATCATT 
                 1951 
               
               
                 NM_053079Musmusculus 
                 CCGTGCTTCAGGCAGGCTGGCTTCTAACTGTGGCGGTCGGCAATATCATT 
                 1930 
               
               
                 XM_007063Homosapiens 
                 CGGTGCTTCAGGCAGGATGGCTGCTGACCGTGGCTGTTGGCAACATCATT 
                 1892 
               
               
                 AY027496Ovis 
                 CGGTACTTCAGGCAGGATGGCTGTTGACCGTGGCCGTTGGCAACATCATC 
                 1972 
               
               
                 U13707Oryctolaguscunic 
                 CGGTGCTGCAGGACCGGTGGCTGCTGACGGTGGCTGTGGGCAACATCATT 
                 1897 
               
               
                 SequencetosubmitGenbak 
                 CGGTGCTTCAGGCGGGATGGCTGCTGACAGTGGCT--------------- 
                 1840 
               
               
                 AY029615Gallusgallus 
                 CAGTGCTGCAAGCAGGATGGCTGCTAACAGTGGCTGTCGGTAACATAATT 
                 1983 
               
               
                   
                 * ** ** ** *   * *****  * ** ***** 
               
               
                   
               
               
                 D50306Rat 
                 GTCCTCATTGTGGCTGAGGCAGGCCACTTCGACAAACAGTGGGCTGAGTA 
                 2001 
               
               
                 NM_053079Musmusculus 
                 GTGCTCATCGTGGCAGGGGCGGGGCACTTCCCCAAACAGTGGGCTGAGTA 
                 1980 
               
               
                 XM_007063Homosapiens 
                 GTGCTCATCGTGGCAGGGGCAGGCCAGTTCAGCAAACAGTGGGCCGAGTA 
                 1942 
               
               
                 AY027496Ovis 
                 GTGCTTATTGTGGCAGGAGCAGGCCAGTTCAGTGAACAGTGGGCCGAGTA 
                 2022 
               
               
                 U13707Oryctolaguscunic 
                 GTGCTCATCGTGGCCGGCGCGGGCCAGATCAACAAGCAGTGGGCCGAGTA 
                 1947 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 GTCCTTATCGTGGCTGGAGCATCCAAACTCAGTGAGCAGTGGGCAGAATA 
                 2033 
               
               
                   
               
               
                 D50306Rat 
                 TGTTCTGTTCGCCTCCTTGCTCCTGGTCGTCTGCATCATATTTGCCATTA 
                 2051 
               
               
                 NM_053079Musmusculus 
                 CATTCTGTTTGCCTCATTGCTTCTGGTTGTCTGCGTGATATTCGCCATCA 
                 2030 
               
               
                 XM_007063Homosapiens 
                 CATTCTATTTGCCGCGTTGCTTCTGGTCGTCTGTGTAATTTTTGCCATCA 
                 1992 
               
               
                 AY027496Ovis 
                 CGTTCTGTTTGCGGCATTGCTTCTGGTCGTCTGCATAATATTTGCCATCA 
                 2072 
               
               
                 U13707Oryctolaguscunic 
                 CATCCTCTTTGCCGCCCTGCTCCTGGTCGTCTGTGTCATATTTGCCATCA 
                 1997 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 TGTTCTCTTTGCTGCCTTGCTTTTTGCAGTTTGCATTATTTTTGCTGTCA 
                 2083 
               
               
                   
               
               
                 D50306Rat 
                 TGGCCCGATTCTACACCTACATCAACCCAGCAGAGATCGAGGCACAGTTC 
                 2101 
               
               
                 NM_053079Musmusculus 
                 TGGCTCGATTCTACACCTACATCAACCCAGCAGAGATTGAAGCACAGTTT 
                 2080 
               
               
                 XM_007063Homosapiens 
                 TGGCTCGGTTCTATACTTACATCAACCCAGCGGAGATCGAAGCTCAATTT 
                 2042 
               
               
                 AY027496Ovis 
                 TGGCTCGATTCTATACGTATGTCAACCCCGCAGAGATTGAAGCTCAGTTT 
                 2122 
               
               
                 U13707Oryctolaguscunic 
                 TGGCTCGATTCTATACGTATGTCAACCCGGCCGAGATCGAGGCTCAGTTT 
                 2047 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 TGGCATATTTTTATACATATACTGATCCAAATGAGGTTGAAGCCCAACTT 
                 2133 
               
               
                   
               
               
                 D50306Rat 
                 GATGAGGATGAGAAGAAAAAGGGCGTAGGGAAGGAA---AACCCGTATTC 
                 2148 
               
               
                 NM_053079Musmusculus 
                 GATGAGGATGAGAAGAAAAAGGGCATAGGAAAGGAA---AACCCGTATTC 
                 2127 
               
               
                 XM_007063Homosapiens 
                 GATGAGGATGAAAAGAAAAACAGACTGGAAAAGAGT---AACCCATATTT 
                 2089 
               
               
                 AY027496Ovis 
                 GATGAGGATGACAAGGAGGATGACCTGGAAAAGAGT---AACCCATACGC 
                 2169 
               
               
                 U13707Oryctolaguscunic 
                 GAAGAAGATGAGAAGAAAAAGAACCCAGAAAAGAAC---GACCTCTACCC 
                 2094 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 GATGAAGAAGAAAAGAAGAAACAAATAAAACAGGATCCAGACTTGCACGG 
                 2183 
               
               
                   
               
               
                 D50306Rat 
                 CTCG---TTGGAACCTGTCTCACAGACAAACATGTGAAGATCAGAAAGCA 
                 2195 
               
               
                 NM_053079Musmusculus 
                 TTCA---TTGGAACCAGTCTCACAGACAAATATGTGAAGGGCAGAAGGCA 
                 2174 
               
               
                 XM_007063Homosapiens 
                 CATG---TCAGGGGCCAATTCACAGAAACAGATGTGAAGGTCAGGAGGCA 
                 2136 
               
               
                 AY027496Ovis 
                 CAAG---CTGGACTTCGTCTCACAGACACAAATGTGAATGTCAGGAAGCA 
                 2216 
               
               
                 U13707Oryctolaguscunic 
                 CTCC---GTGGCGCCCGTCTCACAGACACAGATGTGA--GTCTGGAGGCG 
                 2139 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 AAAAGAATCTGAAGCTGTCTCTCAGATGTAGAAG-GTGTATTCAAGAGCA 
                 2232 
               
               
                   
               
               
                 D50306Rat 
                 AGTGGAGAACATACCAAGTC--CAGCATTCACCATGACCTCTGCCC--AA 
                 2241 
               
               
                 NM_053079Musmusculus 
                 AATTGGAGAAAGATCAAGTT--CAACATGAGCCCTGACCTCTGTCC--AA 
                 2220 
               
               
                 XM_007063Homosapiens 
                 AGTGGAGGATGGACTGGGCC--C-GCAGATGCCCTGACCTCTGCCCCCAG 
                 2183 
               
               
                 AY027496Ovis 
                 AGCGGACGC-GGGGCTGGGC--CAGGGTGTGCCCAGGGGTCTGTCCCATG 
                 2263 
               
               
                 U13707Oryctolaguscunic 
                 -GTGTAGGA-GGCCCACGCC--TGGCGTGCACTGTGACCTCTGTCCGA-G 
                 2184 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 TTTGTAAATCATGGTAGCCTGTTAACTGTCCCTGCAATAACAGGAATCAG 
                 2282 
               
               
                   
               
               
                 D50306Rat 
                 GGGACAGGACCCTCCACCACAGAGTCCTTGCTGGAGAAAGACTTCAGACA 
                 2291 
               
               
                 NM_053079Musmusculus 
                 GGGACAGGACACTCCACCACAGAGTCCCTGATGGAGAAAGACCTCAGAAG 
                 2270 
               
               
                 XM_007063Homosapiens 
                 GTAGCAGGACACTCCATTGGATGGCCCCTGATG-AGGAAGACTTCAGAAT 
                 2232 
               
               
                 AY027496Ovis 
                 GGGGCAGGACACTCTGTTGGGTGGCCTCTGATG-GGGAAGACTTCAGAAC 
                 2312 
               
               
                 U13707Oryctolaguscunic 
                 GGCGCAGGACGTACCCCTGGGCAGCCCCGGAAG-GGGAGGACTTGAGAAC 
                 2233 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 GGTATTGCTGACATCACTGGGTAATATACCTTGTGGGAGAGACTAAGAAA 
                 2332 
               
               
                   
               
               
                 D50306Rat 
                 TGTGAGCCAAAATAATAACAAAGCAGGTTTTCAGGCTGACGGCTGTGAAT 
                 2341 
               
               
                 NM_053079Musmusculus 
                 TGTGAGCCAGAATAATAACAAAGCAGGTTTTCTAACCAACAGCTGTGAAC 
                 2320 
               
               
                 XM_007063Homosapiens 
                 TGGGAACTAAACCATGAATGC--TATTTTCTTTTTTCTTTTTCTTTTCTT 
                 2280 
               
               
                 AY027496Ovis 
                 TGTGGACCAAACCAAGACAGC--TGCTTTCTC-AGCAGCCGGCAATGAAC 
                 2359 
               
               
                 U13707Oryctolaguscunic 
                 TGTGAACCAGACCACGAAAGC--TATGTTCTG-AGCAGCCAGTGATGAGT 
                 2280 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 CACTGTTCTGACTTAACATAC---AGCCTCTTGGGAAGCAAGACGAAATG 
                 2379 
               
               
                   
               
               
                 D50306Rat 
                 CTGAAACTCTAGGGGAGCCTTTTT-------------------------- 
                 2365 
               
               
                 NM_053079Musmusculus 
                 CTGAAACTCTAGGGGAGCCTTTTTTATTTAAAAAAATTTTTTTTTTAATT 
                 2370 
               
               
                 XM_007063Homosapiens 
                 TTTTTTTTTT-------TTTTTTTTTTTGAGACAGAGTTTTGCTCTTGTT 
                 2323 
               
               
                 AY027496Ovis 
                 CTGAAACTCCAAAAGACGTCCTTTT------------------------- 
                 2384 
               
               
                 U13707Oryctolaguscunic 
                 CCAAAACTCTGAAAGAAATCTTGTT------------------------- 
                 2305 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 ATTAATCTCTTGTACAGAAGCTGGC------------------------- 
                 2404 
               
               
                   
               
               
                 D50306Rat 
                 -------------------------------------------------- 
               
               
                 NM_053079Musmusculus 
                 TTTTAAATTTTTTTTATTTTTTATTTTTTTTGCTTGTTTGTTTGTTTCGA 
                 2420 
               
               
                 XM_007063Homosapiens 
                 GTCCAGGCTGGAGTGCAATGGCACGATCTCAGCTCACTGC---------A 
                 2364 
               
               
                 AY027496Ovis 
                 -------------------------------------------------- 
               
               
                 U13707Oryctolaguscunic 
                 -------------------------------------------------- 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 -------------------------------------------------- 
               
               
                   
               
               
                 D50306Rat 
                 -------------------------------------------------- 
               
               
                 NM_053079Musmusculus 
                 GACAGGGTTTCTCGTGTGTAGCCCTTGGTTGTCCTGGAACTCACTCTGTA 
                 2470 
               
               
                 XM_007063Homosapiens 
                 ACCTCCGCCTCCCAGGTTCAAGTAATTCTCCTGCCTCAGCCTCCCGAGTG 
                 2414 
               
               
                 AY027496Ovis 
                 -------------------------------------------------- 
               
               
                 U13707Oryctolaguscunic 
                 -------------------------------------------------- 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 -------------------------------------------------- 
               
               
                   
               
               
                 D50306Rat 
                 -------------------------------------------------- 
               
               
                 NM_053079Musmusculus 
                 GACCAGACTGGCCTCAAACTCAGAAATCCACCTGCCCCTGCCCCTGCCCC 
                 2520 
               
               
                 XM_007063Homosapiens 
                 GCTGGGATTAGCGGCA---------------------------------- 
                 2430 
               
               
                 AY027496Ovis 
                 -------------------------------------------------- 
               
               
                 U13707Oryctolaguscunic 
                 -------------------------------------------------- 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 -------------------------------------------------- 
               
               
                   
               
               
                 D50306Rat 
                 -------------------------------------------------- 
               
               
                 NM_053079Musmusculus 
                 TGCCCCTGCCCCTGCCCCTGCCTCTGCCTCTGCCTCCCAAGTGCTGGATT 
                 2570 
               
               
                 XM_007063Homosapiens 
                 ------TGCACCACCACGCCCAGCTATTTTTGTATTTTTAGTAGAGAT-- 
                 2472 
               
               
                 AY027496Ovis 
                 -------------------------------------------------- 
               
               
                 U13707Oryctolaguscunic 
                 -------------------------------------------------- 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 -------------------------------------------------- 
               
               
                   
               
               
                 D50306Rat 
                 -----------------------------AATTTGTTTTTCTTGAGACAA 
                 2386 
               
               
                 NM_053079Musmusculus 
                 TGGAGGCATGCACCACCATGCCCAGCTATAATTTTTTTTTTTTAAGACAG 
                 2620 
               
               
                 XM_007063Homosapiens 
                 ---GGGGTTTCACCATGTTGGCCAGG-ATGGTCTCGATCTCTTGACCTGG 
                 2518 
               
               
                 AY027496Ovis 
                 -------------------------------------------------- 
               
               
                 U13707Oryctolaguscunic 
                 -------------------------------------------------- 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 -------------------------------------------------- 
               
               
                   
               
               
                 D50306Rat 
                 GGTATCTCTGTGTAACCCTGGCTATCCTGGAACTCACTCTATAGACCAGG 
                 2436 
               
               
                 NM_053079Musmusculus 
                 GGATTCTCTGTATAAGCCTGACTGCCCTGGAACTTGCTCTATAGACCAGG 
                 2670 
               
               
                 XM_007063Homosapiens 
                 TGA---TCTGCCCACCTCGGCCTGCCAAAGTGCTGGGATTACAGGCTTGA 
                 2565 
               
               
                 AY027496Ovis 
                 -------------------------------------------------- 
               
               
                 U13707Oryctolaguscunic 
                 -------------------------------------------------- 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 -----------------------ATCCTGAGGAAACTCCTGCAGAATTTG 
                 2431 
               
               
                   
               
               
                 D50306Rat 
                 CTGGCCTCGAACTCACAGATATCTGTCTGCCTCTGCCTCCTAAGTACTGG 
                 2486 
               
               
                 NM_053079Musmusculus 
                 CTGGCCTTGAACTCACAGAGATCTGCCTGCCTCTTCCTCCTAAGTACTGG 
                 2720 
               
               
                 XM_007063Homosapiens 
                 GCTACCGCGCCCGGCCGTGAACGCTATTTTCTAAGCAGCC--AGCAGTGA 
                 2613 
               
               
                 AY027496Ovis 
                 ------------------------GTTTGTTTGTTTTTAG--AGAAGTCT 
                 2408 
               
               
                 U13707Oryctolaguscunic 
                 ------------------------G------------------AAAGTCT 
                 2313 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 CACTCTTAAAATGTACCTCAAGCTCAATACCATAGCATTA-AAATATTGA 
                 2480 
               
               
                   
               
               
                 D50306Rat 
                 GATTCAAGGCATGTACGGCAACTGCCCAGCTAAAATATTATTTATAACAT 
                 2536 
               
               
                 NM_053079Musmusculus 
                 GATTTCAGGCATGCACCACAACTGCCCAGCTAAAATATTATTTATAATAT 
                 2770 
               
               
                 XM_007063Homosapiens 
                 ATCTAAAACTCTGGAAGAAGTCTTCTGTTTGAAAGGCTTATTTAAGCCAC 
                 2663 
               
               
                 AY027496Ovis 
                 TATTTAAAGCGCACAC-ACACGCACACGCACACA-------------CAT 
                 2444 
               
               
                 U13707Oryctolaguscunic 
                 TATTTAAAACACACAC-ACACACACACACACACA-------------CAC 
                 2349 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 AATTGCACTTGGCACTATTAGACACTCTAAAAAGATGTATTTT----TAT 
                 2526 
               
               
                   
               
               
                 D50306Rat 
                 GCACTTTCTGGGTTTTTTGTTTTTAAAACATACTTTTTTTTTTAACACTG 
                 2586 
               
               
                 NM_053079Musmusculus 
                 GCACTTTCTGG----TTTGTTTTTG--------TTTTTCTTTTAA-ACTG 
                 2807 
               
               
                 XM_007063Homosapiens 
                 ACGTACACACA-----CTGTCTTAGA-------GTACTGTGAGCCCACCC 
                 2701 
               
               
                 AY027496Ovis 
                 GCACACACACA------CACTTTTAT----------AAGAGTCCATACTC 
                 2478  
               
               
                 U13707Oryctolaguscunic 
                 ACACACTTTTC------CAACACTG------------ACAGCCTAC---C 
                 2378 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 ACTGTATTTCAATTTTATAATGTGGAGGGGTGGGGAAAAAGGTGTTGCCA 
                 2576 
               
               
                   
               
               
                 D50306Rat 
                 GGCCATTTCTAACATTTCTGCCACAGAAGTGGATTTAGCTCAGATTAA-- 
                 2634 
               
               
                 NM_053079Musmusculus 
                 GGCTGTATCTTACATTTCTGCCACAGAAATGAACTTAGCTCAGATTAACT 
                 2857 
               
               
                 XM_007063Homosapiens 
                 CACATTGGTCATCTTCCCTATCACACAAATGATGTTATTTTGGACTAGCT 
                 2751 
               
               
                 AY027496Ovis 
                 TGCCTGAACTCCTTTTCCTAACACACAAATAAAGTTATTTTGGACTAACT 
                 2528 
               
               
                 U13707Oryctolaguscunic 
                 CATGTTAACTCCTTCTCTACCAATGCAAATGCTGTTATTTTGGACTAACT 
                 2428 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 AGAAATAGTAATTGAAGCCAAACTGTCTGCGTGACCCTTCTAGCCTCACT 
                 2626 
               
               
                   
               
               
                 D50306Rat 
                 -----TTTTGAAAAGGTAACAGTACTGTTTTTTT-----------TCCTT 
                 2668 
               
               
                 NM_053079Musmusculus 
                 T--AATTTTGAAAAGGCAATAGTATTGTTTTTT---------------CT 
                 2890 
               
               
                 XM_007063Homosapiens 
                 T--AATTTTGAAATGGTAACAAAGTTTCCTATTCCATACTGTTCATTTCT 
                 2799 
               
               
                 AY027496Ovis 
                 TGAATTTTTGAAATGGTGGCCAAGCTCCATACGT-----------GCATT 
                 2567 
               
               
                 U13707Oryctolaguscunic 
                 T-AATTTTGAACACTGTT-CTATGTTGCTTGTAT-----------TC--T 
                 2463 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 GTTACTTGAAAGCAGGTCAC-ATGTGCCTTAAATT---------CTTTTC 
                 2666 
               
               
                   
               
               
                 D50306Rat 
                 AATGCTCTTA-TGAAAACAATGTTGAA-----------------TTTACA 
                 2700 
               
               
                 NM_053079Musmusculus 
                 AACAGTTTTA-TGAAAACAATATTGAA-----------------TTTACA 
                 2922 
               
               
                 XM_007063Homosapiens 
                 AATACTCTTA-CGAAAACTATTCTAAAGGAGGCAGGAGCCAAGGCCAAAA 
                 2848 
               
               
                 AY027496Ovis 
                 CGCACACTCTGTGCAAACAATGTTAAAGGAGGCAAAAAGTGA----ATGG 
                 2613 
               
               
                 U13707Oryctolaguscunic 
                 AACATCCTTAGGAAAGGCAATGTTAAGAGAGGCAGGAGGCAATGCCAAAG 
                 2513 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 TATGTCCTTA---AGAATAATAGGAGAAAG----------------GTTC 
                 2697 
               
               
                   
               
               
                 D50306Rat 
                 GAGGGCTT-------TTTTAGCAGTGTGTAGTGAGTGTCAGCTGATTCGA 
                 2743 
               
               
                 NM_053079Musmusculus 
                 GAGGGCTT-------TTTTAATAGTGTGTAATGAGTATCAACTGATTCAA 
                 2965 
               
               
                 XM_007063Homosapiens 
                 GTGAACGTACAGG--TTTGAAATGGCTGTGATAAGGACCAGCTGGTATTA 
                 2896 
               
               
                 AY027496Ovis 
                 TTGGGGCTTTTGA-ATAGTACGTGTTCATAATAAGGACCGGCTGGTATTA 
                 2662 
               
               
                 U13707Oryctolaguscunic 
                 TTGAATATGTAGGTGTCAGAATGGTATATACCACATATTACTTAGTATTA 
                 2563 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 TTAGATTTC------TCAGATTAAAATGT-CTCTGCTCCACATAGCAGGA 
                 2740 
               
               
                   
               
               
                 D50306Rat 
                 GCTAATAACCTTACCTCGGGGTTTTT----------------------GT 
                 2771 
               
               
                 NM_053079Musmusculus 
                 GCTAATTGCTTTACCTTGGGGTTTTTTTGTTTGTTTGTTTGTTTGTTTGT 
                 3015 
               
               
                 XM_007063Homosapiens 
                 ACTGATAACTTTACCTTTGGGTTTTT----------------------GT 
                 2924 
               
               
                 AY027496Ovis 
                 ACTGATAACTCTACCTTCTGTTTTTA------------------------ 
                 2688 
               
               
                 U13707Oryctolaguscunic 
                 ACTGAAAACCTCAACTTTGAGGTTTT------------------------ 
                 2589 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 ACTTGGACATGCACTGTGATGTGCTT----------------------T 
                 2767 
               
               
                   
               
               
                 D50306Rat 
                 TTCTTTGTTTTCCTGGTCTCCTTTGCCTGACCTCTTTTTAAATTATGTGT 
                 2821 
               
               
                 NM_053079Musmusculus 
                 TTGTTTGTTTTTCTAGTCTCCTTTGCCTTACCTCTTTTTAAATTATGTGT 
                 3065 
               
               
                 XM_007063Homosapiens 
                 TATTTTGTTTTTCTAGTCCCT--------ACCTGTGTTTAAATTATGGAT 
                 2966 
               
               
                 AY027496Ovis 
                 -GTTCTGTTTTT-CCATTCCCT-------ACCTCTTTGTAAATTATGGAT 
                 2729 
               
               
                 U13707Oryctolaguscunic 
                 -GTTCTATTTTTTCCACTCCTT-------ACCTCTTTTTAACCTGTGGAC 
                 2631 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 ATGTGCCTATTATTAACTGCCCATTGGTATGTTCTTAATTAATTGTGT-T 
                 2816 
               
               
                   
               
               
                 D50306Rat 
                 AA---TTCAAAAGACTATTCAAGTGAT-GGTTAGTCATGAGTCGT--GAC 
                 2865 
               
               
                 NM_053079Musmusculus 
                 AA---TTCAAAAGACTA----------------GTCATGAGTTGT--GAA 
                 3094 
               
               
                 XM_007063Homosapiens 
                 AA---CTCGAAAGACAGCTCAGGTGAA-GGCCAGTAATGATTTTTTTGAA 
                 3012 
               
               
                 AY027496Ovis 
                 TAACCTTTGAAAAACCACTCAGGTAAA-GGCAAGTCATGATTTTT--GGA 
                 2776 
               
               
                 U13707Oryctolaguscunic 
                 AA--CTCAAAAGGACCACTCAGATAAA-GGCCAGTAAAGATTTTT--TTT 
                 2676 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 AA----TGGGATGTCCACTGAGGTGAACAGACAATGGCAAATTATATTTT 
                 2862 
               
               
                   
               
               
                 D50306Rat 
                 GTTTGACTGGTGTGAAGTAAATTCTTGTTCTTAAG--------------- 
                 2900 
               
               
                 NM_053079Musmusculus 
                 GTTTCACTGGTCTGAAATAAATTCTAGTTCTTAA---------------- 
                 3128 
               
               
                 XM_007063Homosapiens 
                 GTTTCAATGGTGTGAAATAAATTTCTGTTCTTA----------------- 
                 3045 
               
               
                 AY027496Ovis 
                 GTCTCAACGGTATGAAATAAACTCTCATTCTCAAGAAAAAAAAAAAAAAA 
                 2826 
               
               
                 U13707Oryctolaguscunic 
                 GCCGTTTTG--ATGAAATAAAATAATGTTCCTAAG--------------- 
                 2709 
               
               
                 SequencetosubmitGenbak 
                 -------------------------------------------------- 
               
               
                 AY029615Gallusgallus 
                 GAATAACCACCAAGAATAAAACTTGTGTTGTAACAAAAAAAAAAAAAAAA 
                 2912 
               
               
                   
               
               
                 D50306Rat 
                 --- 
               
               
                 NM_053079Musmusculus 
                 --- 
               
               
                 XM_007063Homosapiens 
                 --- 
               
               
                 AY027496Ovis 
                 AAA 
                 2829 
               
               
                 U13707Oryctolaguscunic 
                 --- 
               
               
                 SequencetosubmitGenbak 
                 --- 
               
               
                 AY029615Gallusgallus 
                 AA- 
                 2914 
               
               
                   
               
             
          
           
               
                 Alignment of Nucleotide Full Length Sequence of Canine and Human 
               
             
          
           
               
                 Sequence 1: SequencetosubmitGenbank 
                 1840 bp 
               
               
                   
               
               
                 Sequence 2: XM_007063Homosapiens 
                 3045 bp 
               
               
                   
               
             
          
           
               
                 Start of Pairwise alignments 
               
               
                   
               
               
                 Aligning. . .  
               
               
                   
               
               
                 Sequences (1:2) Aligned. Score: 85 
               
               
                   
               
               
                 Guide tree file created: 
               
               
                   
               
               
                 [/net/nfs0/vol1/production/w3nobody/tmp/305133.88341-239044.dnd] 
               
               
                   
               
               
                 Start of Multiple Alignment 
               
               
                   
               
               
                 There are 1 groups 
               
               
                   
               
               
                 Aligning. . .  
               
               
                   
               
               
                 Group 1: Sequences: 2 Score:31290 
               
               
                   
               
               
                 Alignment Score 10725 
               
               
                   
               
               
                 CLUSTAL-Alignment file created 
               
               
                   
               
               
                 [/net/nfs0/vol1/production/w3nobody/tmp/305133.88341-239044.aln] 
               
               
                   
               
               
                 Your Multiple Sequence Alignment: 
               
               
                   
               
               
                 305133.88341-239044.aln 
               
               
                   
               
               
                 CLUSTAL W (1.81) multiple sequence alignment 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:7) 
               
             
          
           
               
                 SequencetosubmitGenbank 
                 ----------------------------------------CATCTTCTTC 
                 10 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:1) 
               
             
          
           
               
                 XM_007063Homosapiens 
                 GAATGTCCAAATCACACAGTTTCTTTGGTTATCCCCTGAGCATCTTCTTC 
                 50 
               
               
                   
                                                         ********** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 ATCGTGGTCAATGAGTTCTGTGAAAGATTTTCCTACTATGGAATGAGAGC 
                 60 
               
               
                 XM_007063Homosapiens 
                 ATCGTGGTCAATGAGTTTTGCGAAAGATTTTCCTACTATGGAATGCGAGC 
                 100 
               
               
                   
                 ***************** ** ************************ **** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 ACTCCTGATTCTGTACTTCAGACGGTTCATCGGGTGGGACGATAATCTGT 
                 110 
               
               
                 XM_007063Homosapiens 
                 AATCCTGATTCTGTACTTCACAAATTTCATCAGCTGGGATGATAACCTGT 
                 150 
               
               
                   
                 * ****************** *   ****** * ***** ***** **** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CCACGGCCATCTACCACACGTTTGTGGCTCTGTGCTACCTGACGCCGATC 
                 160 
               
               
                 XM_007063Homosapiens 
                 CCACCGCCATCTACCATACGTTTGTGGCTCTGTGCTACCTGACGCCAATT 
                 200 
               
               
                   
                 **** *********** ***************************** ** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CTCGGCGCACTGATCGCAGACTCCTGGCTGGGAAAGTTCAAGACAATCGT 
                 210 
               
               
                 XM_007063Homosapiens 
                 CTCGGAGCTCTTATCGCCGACTCGTGGCTGGGAAAGTTCAAGACCATTGT 
                 250 
               
               
                   
                 ***** ** ** ***** ***** ******************** ** ** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GTCACTCTCCATTGTCTACACAATTGGACAGGCGGTCACTGCAGTAAGCT 
                 260 
               
               
                 XM_007063Homosapiens 
                 GTCGCTCTCCATTGTCTACACAATTGGACAAGCAGTCACCTCAGTAAGCT 
                 300 
               
               
                   
                 *** ************************** ** *****  ********* 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CAATTAATGACCTCACAGACTATAACAAAGATGGAACTCCTGACAATCTG 
                 310 
               
               
                 XM_007063Homosapiens 
                 CCATTAATGACCTCACAGACCACAACCATGATGGCACCCCCGACAGCCTT 
                 350 
               
               
                   
                 * ****************** * *** * ***** ** ** ****  ** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 TCCGTGCATGTGGCACTGTCCATGATTGGCCTGGCCCTGATAGCTCTGGG 
                 360 
               
               
                 XM_007063Homosapiens 
                 CCTGTGCACGTGGTGCTGTCCTTGATCGGCCTGGCCCTGATAGCTCTCGG 
                 400 
               
               
                   
                  * ***** ****  ****** **** ******************** ** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 AACTGGAGGAATAAAGCCCTGTGTGTCTGCATTTGGTGGAGACCAGTTTG 
                 410 
               
               
                 XM_007063Homosapiens 
                 GACTGGAGGAATCAAACCCTGTGTGTCTGCGTTTGGTGGAGATCAGTTTG 
                 450 
               
               
                   
                  *********** ** ************** *********** ******* 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 AAGAGGGCCAGGAAAAACAAAGAAACAGATTCTTTTCCATCTTTTATTTG 
                 460 
               
               
                 XM_007063Homosapiens 
                 AAGAGGGCCAGGAGAAACAAAGAAACAGATTTTTTTCCATCTTTTACTTG 
                 500 
               
               
                   
                 ************* ***************** ************** *** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GCCATTAATGCTGGAAGCTTGATTTCCACTATTGTCACTCCCATGCTCAG 
                 510 
               
               
                 XM_007063Homosapiens 
                 GCTATTAATGCTGGAAGTTTGCTTTCCACAATCATCACACCCATGCTCAG 
                 550 
               
               
                   
                 ** ************** *** ******* **  **** *********** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 AGTTCACGAATGTGGAATTTACAGTCAGAAAGCTTGTTACCCACTGGCAT 
                 560 
               
               
                 XM_007063Homosapiens 
                 AGTTCAACAATGTGGAATTCACAGTAAACAAGCTTGTTACCCACTGGCCT 
                 600 
               
               
                   
                 ******  *********** ***** *  ******************* * 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 TTGGGGTTCCTGCTGCTCTCATGGCCGTATCTCTGATTGTATTTGTCATT 
                 610 
               
               
                 XM_007063Homosapiens 
                 TTGGGGTTCCTGCTGCTCTCATGGCTGTAGCCCTGATTGTGTTTGTCCTT 
                 650 
               
               
                   
                 ************************* *** * ******** ****** ** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GGCAGTGGAATGTACAAGAAGTTTCAGCCCCAGGGTAATGTCATGGGTAA 
                 660 
               
               
                 XM_007063Homosapiens 
                 GGCAGTGGGATGTACAAGAAGTTCAAGCCACAGGGCAACATCATGGGTAA 
                 700 
               
               
                   
                 ******** **************  **** ***** **  ********** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 AGTTGTCAAGTGCATTGGTTTTGCCCTCAAAAATAGGTTTAGGCACCGGA 
                 710 
               
               
                 XM_007063Homosapiens 
                 AGTGGCCAAGTGCATCGGTTTTGCCATCAAAAATAGATTTAGGCATCGGA 
                 750 
               
               
                   
                 *** * ********* ********* ********** ******** **** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GTAAGCAGTTTCCCAAGAGGGAGCACTGGCTGGACTGGGCTAAAGAGAAA 
                 760 
               
               
                 XM_007063Homosapiens 
                 GTAAGGCATTTCCCAAGAGGGAGCACTGGCTGGACTGGGCTAAAGAGAAA 
                 800 
               
               
                   
                 *****   ****************************************** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 TACGATGAGCGGCTCATCTCTCAAATTAAGATGGTCACAAAAGTGATGTT 
                 810 
               
               
                 XM_007063Homosapiens 
                 TACGATGAGCGGCTCATCTCCCAAATTAAGATGGTTACGAGGGTGATGTT 
                 850 
               
               
                   
                 ******************** ************** ** *  ******** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CTTGTACATCCCACTCCCAATGTTCTGGGCCCTGTTTGACCAGCAGGGCT 
                 860 
               
               
                 XM_007063Homosapiens 
                 CCTGTATATTCCACTCCCAATGTTCTGGGCCTTGTTTGACCAGCAGGGCT 
                 900 
               
               
                   
                 * **** ** ********************* ****************** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CCAGGTGGACACTGCAAGCAACAGCTATGAGTGGGAAAATTGGACTTCTT 
                 910 
               
               
                 XM_007063Homosapiens 
                 CCAGGTGGACACTGCAGGCAACAACTATGTCCGGGAAAATCGGAGCTCTT 
                 950 
               
               
                   
                 **************** ****** *****   ******** ***  **** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GAAGTTCAGCCAGATCAGATGCAGACTGTGAATGCCATCTTGATTGTCGT 
                 960 
               
               
                 XM_007063Homosapiens 
                 GAAATTCAGCCCGATCAGATGCAGACCGTGAACGCCATCCTGATCGTGAT 
                 1000 
               
               
                   
                 *** ******* ************** ***** ****** **** **  * 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CATGGTCCCCATCATGGATGCCGTGGTGTACCCTCTGATTGCAAAATGTG 
                 1010 
               
               
                 XM_007063Homosapiens 
                 CATGGTCCCGATCTTCGATGCTGTGCTGTACCCTCTCATTGCAAAATGTG 
                 1050 
               
               
                   
                 ********* *** * ***** *** ********** ************* 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GCTTCAATTTCACCTCCTTGAAGAGGATGACAGTTGGAATGTTCCTGGCT 
                 1060 
               
               
                 XM_007063Homosapiens 
                 GCTTCAATTTCACCTCCTTGAAGAAGATGGCAGTTGGCATGGTCCTGGCC 
                 1100 
               
               
                   
                 ************************ **** ******* *** ******* 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 TCCATGGCCTTCGTGATGGCGGCGATTGTTCAGCTGGAAATTGATAAAAC 
                 1110 
               
               
                 XM_007063Homosapiens 
                 TCCATGGCCTTTGTGGTGGCTGCCATCGTGCAGGTGGAAATCGATAAAAC 
                 1150 
               
               
                   
                 *********** *** **** ** ** ** *** ******* ******** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 TCTTCCAGTCTTCCCCAAACAAAATGAAGTCCAAATCAAAGTACTGAATA 
                 1160 
               
               
                 XM_007063Homosapiens 
                 TCTTCCAGTCTTCCCCAAAGGAAACGAAGTCCAAATTAAAGTTTTGAATA 
                 1200 
               
               
                   
                 *******************  *** *********** *****  ****** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 TAGGAAATGGTGCCATGAATGTATCTTTTCCTGGAGCGGTGGTGACAGTT 
                 1210 
               
               
                 XM_007063Homosapiens 
                 TAGGAAACAATACCATGAATATATCTCTTCCTGGAGAGATGGTGACACTT 
                 1250 
               
               
                   
                 *******   * ******** ***** ********* * ******** ** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 AGCCAAATGAGTCAATCAGATGGATTTATGACTTTTGATGTAGACAAACT 
                 1260 
               
               
                 XM_007063Homosapiens 
                 GGCCCAATGTCTCAAACAAATGCATTTATGACTTTTGATGTAAACAAACT 
                 1300 
               
               
                   
                  *** ****  **** ** *** ******************* ******* 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GACAAGTATAAACATTTCTTCCACTGGATCACCAGTCATTCCAGTGACTT 
                 1310 
               
               
                 XM_007063Homosapiens 
                 GACAAGGATAAACATTTCTTCTCCTGGATCACCAGTCACTGCTGTAACTG 
                 1350 
               
               
                   
                 ****** **************  *************** * * ** *** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 ATAACTTTGAGCAGGGCCATCGCCATACCCTTCTAGTATGGGCCCCCAAT 
                 1360 
               
               
                 XM_007063Homosapiens 
                 ACGACTTCAAGCAGGGCCAACGCCACACGCTTCTAGTGTGGGCCCCCAAT 
                 1400 
               
               
                   
                 *  ****  ********** ***** ** ******** ************ 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 AATTACCGAGTGGTAAAGGATGGCCTTAACCAGAAGCCAGAAAAAGGAGA 
                 1410 
               
               
                 XM_007063Homosapiens 
                 CACTACCAGGTGGTAAAGGATGGTCTTAACCAGAAGCCAGAAAAAGGGGA 
                 1450 
               
               
                   
                  * ****  ************** *********************** ** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 AAATGGAATCAGATTTATAAATAGTCTTAATGAGAGCCTCAACATCACCA 
                 1460 
               
               
                 XM_007063Homosapiens 
                 AAATGGAATCAGATTTGTAAATACTTTTAACGAGCTCATCACCATCACAA 
                 1500 
               
               
                   
                 **************** ****** * **** ***  * *** ****** * 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 TGGGCGACAAAGTTTATGTGAATGTCACCAGTCACAATGCCAGCGAGTAT 
                 1510 
               
               
                 XM_007063Homosapiens 
                 TGAGTGGGAAAGTTTATGCAAACATCAGCAGCTACAATGCCAGCACATAC 
                 1550 
               
               
                   
                 ** * *  **********  **  *** ***  ***********   ** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CAGTTCTTTTCTTTGGGCACAAAAAACATTACAATAAGTTCAACACAACA 
                 1560 
               
               
                 XM_007063Homosapiens 
                 CAGTTTTTTCCTTCTGGCATAAAAGGCTTCACAATAAGCTCAACAG---A 
                 1597 
               
               
                   
                 ***** *** ***  **** ****  * * ******** ******    * 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GATCTCACAAAATTGTACAAAAGTTCTCCAATCATCCAACCTTGAATTTG 
                 1610 
               
               
                 XM_007063Homosapiens 
                 GATTCCGCCACAATGTCAACCTAATTTCAATACTTTCTACCTTGAATTTG 
                 1647 
               
               
                   
                 ***  * * * * ***  *     * ** *  * * * ************ 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GTAGTGCATATACCTATGTAATCGGAACGCAGAGCACTGGCTGCCCTGAA 
                 1660 
               
               
                 XM_007063Homosapiens 
                 GTAGTGCTTATACCTATATAGTCCAAAGGAAGAATGACAGCTGCCCTGAA 
                 1697 
               
               
                   
                 ******* ********* ** **  ** * ***      *********** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 TTGCATATGTTTGAAGATATTTCACCCAACACAGTTAACATGGCTCTGCA 
                 1710 
               
               
                 XM_007063Homosapiens 
                 GTGAAGGTGTTTGAAGATATTTCAGCCAACACAGTTAACATGGCTCTGCA 
                 1747 
               
               
                   
                  ** *  ***************** ************************* 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 GATCCCGCAGTACTTCCTCATCACCTGCGGCGAGGTGGTTTTCTCTGTCA 
                 1760 
               
               
                 XM_007063Homosapiens 
                 AATCCCGCAGTATTTTCTTCTCACCTGTGGCGAAGTGGTCTTCTCTGTCA 
                 1797 
               
               
                   
                  *********** ** **  ******* ***** ***** ********** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CAGGACTGGAGTTCTCATATTCTCAGGCCCCCTCCAACATGAAGTCGGTG 
                 1810 
               
               
                 XM_007063Homosapiens 
                 CGGGATTGGAATTCTCATATTCTCAGGCTCCTTCCAACATGAAGTCGGTG 
                 1847 
               
               
                   
                 * *** **** ***************** ** ****************** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 CTTCAGGCGGGATGGCTGCTGACAGTGGCT-------------------- 
                 1840 
               
               
                 XM_007063Homosapiens 
                 CTTCAGGCAGGATGGCTGCTGACCGTGGCTGTTGGCAACATCATTGTGCT 
                 1897 
               
               
                   
                 ******** ************** ****** 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CATCGTGGCAGGGGCAGGCCAGTTCAGCAAACAGTGGGCCGAGTACATTC 
                 1947 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 TATTTGCCGCGTTGCTTCTGGTCGTCTGTGTAATTTTTGCCATCATGGCT 
                 1997 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CGGTTCTATACTTACATCAACCCAGCGGAGATCGAAGCTCAATTTGATGA 
                 2047 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 GGATGAAAAGAAAAACAGACTGGAAAAGAGTAACCCATATTTCATGTCAG 
                 2097 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 GGGCCAATTCACAGAAACAGATGTGAAGGTCAGGAGGCAAGTGGAGGATG 
                 2147 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 GACTGGGCCCGCAGATGCCCTGACCTCTGCCCCCAGGTAGCAGGACACTC 
                 2197 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CATTGGATGGCCCCTGATGAGGAAGACTTCAGAATTGGGAACTAAACCAT 
                 2247 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 GAATGCTATTTTCTTTTTTCTTTTTCTTTTCTTTTTTTTTTTTTTTTTTT 
                 2297 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 TTTTGAGACAGAGTTTTGCTCTTGTTGTCCAGGCTGGAGTGCAATGGCAC 
                 2347 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 GATCTCAGCTCACTGCAACCTCCGCCTCCCAGGTTCAAGTAATTCTCCTG 
                 2397 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CCTCAGCCTCCCGAGTGGCTGGGATTAGCGGCATGCACCACCACGCCCAG 
                 2447 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CTATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGAT 
                 2497 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 GGTCTCGATCTCTTGACCTGGTGATCTGCCCACCTCGGCCTGCCAAAGTG 
                 2547 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CTGGGATTACAGGCTTGAGCTACCGCGCCCGGCCGTGAACGCTATTTTCT 
                 2597 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 AAGCAGCCAGCAGTGAATCTAAAACTCTGGAAGAAGTCTTCTGTTTGAAA 
                 2647 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 GGCTTATTTAAGCCACACGTACACACACTGTCTTAGAGTACTGTGAGCCC 
                 2697 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 ACCCCACATTGGTCATCTTCCCTATCACACAAATGATGTTATTTTGGACT 
                 2747 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 AGCTTAATTTTGAAATGGTAACAAAGTTTCCTATTCCATACTGTTGATTT 
                 2797 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CTAATACTCTTACGAAAACTATTCTAAAGGAGGCAGGAGCCAAGGCCAAA 
                 2847 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 AGTGAACGTACAGGTTTGAAATGGCTGTGATAAGGACCAGCTGGTATTAA 
                 2897 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CTGATAACTTTACCTTTGGGTTTTTGTTATTTTGTTTTTCTAGTCCCTAC 
                 2947 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 -------------------------------------------------- 
               
               
                 XM_007063Homosapiens 
                 CTGTGTTTAAATTATGGATAACTCGAAAGACAGCTCAGGTGAAGGCCAGT 
                 2997 
               
               
                   
               
               
                 SequencetosubmitGenbank 
                 ------------------------------------------------ 
               
               
                 XM_007063Homosapiens 
                 AATGATTTTTTTGAAGTTTCAATGGTGTGAAATAAATTTCTGTTCTTA 
                 3045 
               
               
                   
               
             
          
           
               
                 Protein Sequence of Canine 
               
               
                   
               
             
          
           
               
                 5′3′ Frame 2 
                   
               
               
                   
               
               
                 catcttcttcatcgtggtcaatgagttctgtgaaagattttcctactatggaatgagagca 
                 (SEQ ID NO:8) 
               
               
                   I  F  F  I  V  V  N  E  F  C  E  R  F  S  Y  Y  G  M  R  A  
                 (SEQ ID NO:13) 
               
               
                   
               
               
                 ctcctgattctgtacttcagacggttcatcgggtgggacgataatctgtccacggccatc 
               
               
                  L  L  I  L  Y  F  R  R  F  I  G  W  D  D  N  L  S  T  A  I 
               
               
                   
               
               
                 taccacacgtttgtggctctgtgctacctgacgccgatcctcggcgcactgatcgcagac 
               
               
                  Y  H  T  F  V  A  L  C  Y  L  T  P  I  L  G  A  L  I  A  D 
               
               
                   
               
               
                 tcctggctgggaaagttcaagacaatcgtgtcactctccattgtctacacaattggacag 
               
               
                  S  W  L  G  K  F  K  T  I  V  S  L  S  I  V  Y  T  I  G  Q 
               
               
                   
               
               
                 gcggtcactgcagtaagctcaattaatgacctcacagactataacaaagatggaactcct 
               
               
                  A  V  T  A  V  S  S  I  N  D  L  T  D  Y  N  K  D  G  T  P 
               
               
                   
               
               
                 gacaatctgtccgtgcatgtggcactgtccatgattggcctggccctgatagctctggga 
               
               
                  D  N  L  S  V  H  V  A  L  S  M  I  G  L  A  L  I  A  L  G 
               
               
                   
               
               
                 actggaggaataaagccctgtgtgtctgcatttggtggagaccagtttgaagagggccag 
               
               
                  T  G  G  I  K  P  C  V  S  A  F  G  G  D  Q  F  E  E  G  Q 
               
               
                   
               
               
                 gaaaaacaaagaaacagattcttttccatcttttatttggccattaatgctggaagcttg 
               
               
                  E  K  Q  R  N  R  F  F  S  I  F  Y  L  A  I  N  A  G  S  L 
               
               
                   
               
               
                 atttccactattgtcactcccatgctcagagttcacgaatgtggaatttacagtcagaaa 
               
               
                  I  S  T  I  V  T  P  M  L  R  V  H  E  C  G  I  Y  S  Q  K 
               
               
                   
               
               
                 gcttgttacccactggcatttggggttcctgctgctctcatggccgtatctctgattgta 
               
               
                  A  C  Y  P  L  A  F  G  V  P  A  A  L  M  A  V  S  L  I  V 
               
               
                   
               
               
                 tttgtcattggcagtggaatgtacaagaagtttcagccccagggtaatgtcatgggtaaa 
               
               
                  F  V  I  G  S  G  M  Y  K  K  F  Q  P  Q  G  N  V  M  G  K 
               
               
                   
               
               
                 gttgtcaagtgcattggttttgccctcaaaaataggtttaggcaccggagtaagcagttt 
               
               
                  V  V  K  C  I  G  F  A  L  K  N  R  F  R  H  R  S  K  Q  F 
               
               
                   
               
               
                 cccaagagggagcactggctggactgggctaaagagaaatacgatgagcggctcatctct 
               
               
                  P  K  R  E  H  W  L  D  W  A  K  E  K  Y  D  E  R  L  I  S 
               
               
                   
               
               
                 caaattaagatggtcacaaaagtgatgttcttgtacatcccactcccaatgttctgggcc 
               
               
                  Q  I  K  M  V  T  K  V  M  F  L  Y  I  P  L  P  M  F  W  A 
               
               
                   
               
               
                 ctgtttgaccagcagggctccaggtggacactgcaagcaacagctatgagtgggaaaatt 
               
               
                  L  F  D  Q  Q  G  S  R  W  T  L  Q  A  T  A  M  S  G  K  I 
               
               
                   
               
               
                 ggacttcttgaagttcagccagatcagatgcagactgtgaatgccatcttgattgtcgtc 
               
               
                  G  L  L  E  V  Q  P  D  Q  M  Q  T  V  N  A  I  L  I  V  V 
               
               
                   
               
               
                 atggtccccatcatggatgccgtggtgtaccctctgattgcaaaatgtggcttcaatttc 
               
               
                  M  V  P  I  M  D  A  V  V  Y  P  L  I  A  K  C  G  F  N  F 
               
               
                   
               
               
                 acctccttgaagaggatgacagttggaatgttcctggcttccatggccttcgtgatggcg 
               
               
                  T  S  L  K  R  M  T  V  G  M  F  L  A  S  M  A  F  V  M  A 
               
               
                   
               
               
                 gcgattgttcagctggaaattgataaaactcttccagtcttccccaaacaaaatgaagtc 
               
               
                  A  I  V  Q  L  E  I  D  K  T  L  P  V  F  P  K  Q  N  E  V 
               
               
                   
               
               
                 caaatcaaagtactgaatataggaaatggtgccatgaatgtatcttttcctggagcggtg 
               
               
                  Q  I  K  V  L  N  I  G  N  G  A  M  N  V  S  F  P  G  A  V 
               
               
                   
               
               
                 gtgacagttagccaaatgagtcaatcagatggatttatgacttttgatgtagacaaactg 
               
               
                  V  T  V  S  Q  M  S  Q  S  D  G  F  M  T  F  D  V  D  K  L 
               
               
                   
               
               
                 acaagtataaacatttcttccactggatcaccagtcattccagtgacttataactttgag 
               
               
                  T  S  I  N  I  S  S  T  G  S  P  V  I  P  V  T  Y  N  F  E 
               
               
                   
               
               
                 cagggccatcgccatacccttctagtatgggcccccaataattaccgagtggtaaaggat 
               
               
                  Q  G  H  R  H  T  L  L  V  W  A  P  N  N  Y  R  V  V  K  D 
               
               
                   
               
               
                 ggccttaaccagaagccagaaaaaggagaaaatggaatcagatttataaatagtcttaat 
               
               
                  G  L  N  Q  K  P  E  K  G  E  N  G  I  R  F  I  N  S  L  N 
               
               
                   
               
               
                 gagagcctcaacatcaccatgggcgacaaagtttatgtgaatgtcaccagtcacaatgcc 
               
               
                  E  S  L  N  I  T  M  G  D  K  V  Y  V  N  V  T  S  H  N  A 
               
               
                   
               
               
                 agcgagtatcagttcttttctttgggcacaaaaaacattacaataagttcaacacaacag 
               
               
                  S  E  Y  Q  F  F  S  L  G  T  K  N  I  T  I  S  S  T  Q  Q 
               
               
                   
               
               
                 atctcacaaaattgtacaaaagttctccaatcatccaaccttgaatttggtagtgcatat 
               
               
                  I  S  Q  N  C  T  K  V  L  Q  S  S  N  L  E  F  G  S  A  Y 
               
               
                   
               
               
                 acctatgtaatcggaacgcagagcactggctgccctgaattgcatatgtttgaagatatt 
               
               
                  T  Y  V  I  G  T  Q  S  T  G  C  P  E  L  H  M  F  E  D  I 
               
               
                   
               
               
                 tcacccaacacagttaacatggctctgcagatcccgcagtacttcctcatcacctgcggc 
               
               
                  S  P  N  T  V  N  M  A  L  Q  I  P  Q  Y  F  L  I  T  C  G 
               
               
                   
               
               
                 gaggtggttttctctgtcacaggactggagttctcatattctcaggccccctccaacatg 
               
               
                  E  V  V  F  S  V  T  G  L  E  F  S  Y  S  Q  A  P  S  N  M 
               
               
                   
               
               
                 aagtcggtgcttcaggcgggatggctgctgacagtggcttgttggcaacatcattgtgct 
               
               
                  K  S  V  L  Q  A  G  W  L  L  T  V  A  C  W  Q  H  H  C  A 
               
               
                   
               
               
                 cattgtggcaggagcaggccagttcagtgaacagtgggctgaatacatcctatttgcggc 
               
               
                  H  C  G  R  S  R  P  V  Q  -  T  V  G  -  I  H  P  I  C  G 
               
               
                   
               
               
                 attgcttctggttgtctgtgtaatatttgccatcatggcccggttttacacttacgtcaa 
               
               
                  I  A  S  G  C  L  C  N  I  C  H  H  G  P  V  L  H  L  R  Q 
               
               
                   
               
               
                 tccagcagagattg 
               
               
                  S  S  R  D   
               
               
                   
               
             
          
           
               
                 5′3′ Frame 2 
                 (SEQ ID NO:13) 
               
               
                   
               
             
          
           
               
                 IFFIVVNEFCERFSYYGMRALLILYFRRFIGWDDNLSTAIYHTFVALCYLTPILGALIAD 
               
               
                   
               
               
                 SWLGKFKTIVSLSIVYTIGQAVTAVSSINDLTDYNKDGTPDNLSVHVALSMIGLALIALG 
               
               
                   
               
               
                 TGGIKPCVSAFGGDQFEEGQEKQRNRFFSIFYLAINAGSLISTIVTPMLRVHECGIYSQK 
               
               
                   
               
               
                 ACYPLAFGVPAALMAVSLIVFVIGSGMYKKFQPQGNVMGKVVKCIGFALKNRFRHRSKQF 
               
               
                   
               
               
                 PKREHWLDWAKEKYDERLISQIKMVTKVMFLYIPLPMFWALFDQQGSRWTLQATAMSGKI 
               
               
                   
               
               
                 GLLEVQPDQMQTVNAILIVVMVPIMDAVVYPLIAKCGFNFTSLKRMTVGMFLASMAFVMA 
               
               
                   
               
               
                 AIVQLEIDKTLPVFPKQNEVQIKVLNIGNGAMNVSFPGAVVTVSQMSQSDGFMTFDVDKL 
               
               
                   
               
               
                 TSINISSTGSPVIPVTYNFEQGHRHTLLVWAPNNYRVVKDGLNQKPEKGENGIRFINSLN 
               
               
                   
               
               
                 ESLNITMGDKVYVNVTSHNASEYQFFSLGTKNITISSTQQISQNCTKVLQSSNLEFGSAY 
               
               
                   
               
               
                 TYVIGTQSTGCPELHMFEDISPNTVNMALQIPQYFLITCGEVVFSVTGLEFSYSQAPSNM 
               
               
                   
               
               
                 KSVLQAGWLLTVACWQHHCAHCGRSRPVQ-TVG-IHPICGIASGCLCNICHHGPVLHLRQ 
               
               
                   
               
               
                 SSRD 
               
               
                   
               
             
          
           
               
                 Multiple Alignment of Amino-Acid Sequences 
               
               
                   
               
             
          
           
               
                 Sequence 1: Caninesubmitted 
                 662 aa 
               
               
                   
               
               
                 Sequence 2: XM_007063HomosapiensProteinSeq 
                 706 aa 
               
               
                   
               
               
                 Sequence 3: DS0306RatProteinSequence 
                 710 aa 
               
               
                   
               
               
                 Sequence 4: NM_053079MusmusculusProteinSeq 
                 709 aa 
               
               
                   
               
               
                 Sequence 5: AY027496Ovis 
                 707 aa 
               
               
                   
               
               
                 Sequence 6: U13707OryctolaguscunicProteinS 
                 707 aa 
               
               
                   
               
               
                 Sequence 7: AY029615GallusgallusProteinSeq 
                 714 aa 
               
               
                   
               
             
          
           
               
                 Start of Pairwise alignments 
               
               
                   
               
               
                 Aligning . . .  
               
               
                   
               
               
                 Sequences (1:2) Aligned. Score: 76 
               
               
                   
               
               
                 Sequences (2:3) Aligned. Score: 84 
               
               
                   
               
               
                 Sequences (3:4) Aligned. Score: 91 
               
               
                   
               
               
                 Sequences (4:5) Aligned. Score: 80 
               
               
                   
               
               
                 Sequences (1:3) Aligned. Score: 77 
               
               
                   
               
               
                 Sequences (2:4) Aligned. Score: 83 
               
               
                   
               
               
                 Sequences (3:5) Aligned. Score: 82 
               
               
                   
               
               
                 Sequences (4:6) Aligned. Score: 76 
               
               
                   
               
               
                 Sequences (1:4) Aligned. Score: 75 
               
               
                   
               
               
                 Sequences (2:5) Aligned. Score: 82 
               
               
                   
               
               
                 Sequences (3:6) Aligned. Score: 77 
               
               
                   
               
               
                 Sequences (4:7) Aligned. Score: 63 
               
               
                   
               
               
                 Sequences (1:5) Aligned. Score: 77 
               
               
                   
               
               
                 Sequences (2:6) Aligned. Score: 80 
               
               
                   
               
               
                 Sequences (1:6) Aligned. Score: 72 
               
               
                   
               
               
                 Sequences (3:7) Aligned. Score: 64 
               
               
                   
               
               
                 Sequences (5:6) Aligned. Score: 77 
               
               
                   
               
               
                 Sequences (1:7) Aligned. Score: 60 
               
               
                   
               
               
                 Sequences (2:7) Aligned. Score: 63 
               
               
                   
               
               
                 Sequences (6:7) Aligned. Score: 61 
               
               
                   
               
               
                 Sequences (5:7) Aligned. Score: 64 
               
               
                   
               
               
                 Guide tree file created: 
               
               
                   
               
               
                 [/net/nfs0/vol1/production/w3nobody/tmp/936042.678539-441485.dnd] 
               
               
                   
               
               
                 Start of Multiple Alignment 
               
               
                   
               
               
                 There are 6 groups 
               
               
                   
               
               
                 Aligning. . .  
               
               
                   
               
               
                 Group 1: Sequences: 2 Score:14016 
               
               
                   
               
               
                 Group 2: Sequences: 2 Score:14858 
               
               
                   
               
               
                 Group 3: Sequences: 4 Score:13893 
               
               
                   
               
               
                 Group 4: Sequences: 5 Score:14022 
               
               
                   
               
               
                 Group 5: Sequences: 6 Score:12718 
               
               
                   
               
               
                 Group 6: Sequences: 7 Score:12338 
               
               
                   
               
               
                 Alignment Score 68091 
               
               
                   
               
               
                 CLUSTAL-Alignment file created 
               
               
                   
               
               
                 [/net/nfs0/vol1/production/w3nobody/tmp/936042.678539-441485.aln] 
               
               
                   
               
               
                 Your Multiple Sequence Alignment: 
               
               
                   
               
               
                 936042.678539-441485.aln 
               
               
                   
               
               
                 CLUSTAL W (1.81) multiple sequence alignment 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:14) 
               
             
          
           
               
                 XM_007063HomosapiensProteinSeq 
                 ---MSKSHS-----FFGYPLSIFFIVVNEFCERFSYYGMRAILILYFTNF 
                 42 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:18) 
               
             
          
           
               
                 U13707OryctolaguscunicProteinS 
                 -MGMSKSLS-----CFGYPLSIFFIVVNEFCERFSYYGMRALLILYFRNF 
                 44 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:15) 
               
             
          
           
               
                 D50306RatProteinSequence 
                 -MGMSKSRG-----CFGYPLSIFFIVVNEFCERFSYYGMRALLVLYFRNF 
                 44 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:16) 
               
             
          
           
               
                 NM_053079MusmusculusProteinSeq 
                 -MGMSKSRG-----CFGYPLSIFFIVVNEFCERFSYYGMRALLVLYFRNF 
                 44 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:17) 
               
             
          
           
               
                 AY027496Ovis 
                 -MGMSVPKS-----CFGYPLSIFFIVVNEFCERFSYYGMRALLILYFQRF 
                 44 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:13) 
               
             
          
           
               
                 Caninesubmitted 
                 ---------------------IFFIVVNEFCERFSYYGMRALLILYFRRF 
                 29 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:19) 
               
             
          
           
               
                 Ay029615GallusgallusProteinSeq 
                 MAAKSKSKGRSVPNCFGYPLSIFFIVINEFCERFSYYGMRAVLVLYFKYF 
                 50 
               
               
                   
                                      *****:*             :*:* 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 ISWDDNLSTAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIG 
                 92 
               
               
                 U13707OryctolaguscunicProteinS 
                 IGWDDNLSTVIYHTFVALCYLTPILGALIADAWLGKFKTIVWLSIVYTIG 
                 94 
               
               
                 D50306RatProteinSequence 
                 LGWDDDLSTAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIG 
                 94 
               
               
                 NM_053079MusmusculusProteinSeq 
                 LGWDDNLSTAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIG 
                 94 
               
               
                 AY027496Ovis 
                 LGWNDNLGTAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIG 
                 94 
               
               
                 Caninesubmitted 
                 IGWDDNLSTAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIG 
                 79 
               
               
                 AY029615GallusgallusProteinSeq 
                 LRWDDNFSTATYHIFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIG 
                 100 
               
               
                   
                 : *:*::.*.*********************:********* ******** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 QAVTSVSSINDLTDHNHDGTPDSLPVHVVLSLIGLALIALGTGGIKPCVS 
                 142 
               
               
                 U13707OryctolaguscunicProteinS 
                 QAVTSLSSVNELTDNNHDGTPDSLPVHVAVCMIGLLLIALGTGGIKPCVS 
                 144 
               
               
                 D50306RatProteinSequence 
                 QAVTSVSSINDLTDHDHDGSPNNLPLHVALSMIGLALIALGTGGIKPCVS 
                 144 
               
               
                 NM_053079MusmusculusProteinSeq 
                 QAVISVSSINDLTDHDHNGSPDSLPVHVALSMVGLALIALGTGGIKPCVS 
                 144 
               
               
                 AY027496Ovis 
                 QVVTAVSSINDLTDFNHDGTPNNISVHVALSMIGLVLIALGTGGIKPCVS 
                 144 
               
               
                 Caninesubmitted 
                 QAVTAVSSTNDLTDYNKDGTPDNLSVHLALSMIGLALIALGTGGIKPCVS 
                 129 
               
               
                 AY029615GallusgallusProteinSeq 
                 QAVMAVSSINDMTDQNRDGNPDNIAVHIALSMTGLILIALGTGGIKPCVS 
                 150 
               
               
                   
                 *.* ::**:*::** :::*.*:.:.:*:.:.: ** ************** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 AFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTIITPMLRVQQCGIHSK 
                 192 
               
               
                 U13707OryctolaguscunicProteinS 
                 AFGGDQFEEGQEKQRNRFFSTFYLAINAGSLLSTIITPMLRVQQCGIHSK 
                 194 
               
               
                 D50306RatProteinSequence 
                 AFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTIITPILRVQQCGIHSQ 
                 194 
               
               
                 NM_053079MusmusculusProteinSeq 
                 AFGGDQFEEGQEKQRNRFFSIFYLAINGGSLLSTIITPILRVQQCGIHSQ 
                 194 
               
               
                 AY027496Ovis 
                 AFGGDQFEEGQEKQRNRFFSIFYLAINAGSLLSTIITPMLRVQVCGIHSK 
                 194 
               
               
                 Caninesubmitted 
                 AFGGDQFEEGQEKQRNRFFSTFYLAINAGSLISTIVTPMLRVHECGIYSQ 
                 179 
               
               
                 AY029615GallusgallusProteinSeq 
                 AFGGDQFEEHQEKQRSRFFSIFYLSINAGSLISTIITPILRAQECGIHSR 
                 200 
               
               
                   
                 ********* *****.********:**.***:***:**::*.: ***: : 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 QACYPLAFGVPAALMAVALTVFVLGSGMYKKFKPQGNIMGKVAKCIGFAI 
                 242 
               
               
                 U13707OryctolaguscunicProteinS 
                 QACYPLAFGIPAILMAVSLIVFIIGSGMYKKFKPQGNILSKVVKCICFAI 
                 244 
               
               
                 D50306RatProteinSequence 
                 QACYPLAFGVPAALMAVALIVFVLGSGMYKKFQPGNIMGKVAKRCIGFAI 
                 244 
               
               
                 NM_053079MusmusculusProteinSeq 
                 QACYPLAFGVPAALMAVALIVFVLGSGMYKKFQPQGNIMGKVAKCIGFAI 
                 244 
               
               
                 AY027496Ovis 
                 QACYPLAFGVPAALMAVSLIVEVIGSGMYKKFQPGNIMSKVARKCIGFAI 
                 244 
               
               
                 Caninesubmitted 
                 KACYPLAFGVPAALMAVSLIVFVIGSGMYKKFQPQGNVMGKVVKCIGFAL 
                 229 
               
               
                 AY029615GallusgallusProteinSeq 
                 QQCYPLAFGVPAALMAVSLVVFIAGSGMYKKVQPQGNIMVRVCKCIGFAI 
                 250 
               
               
                   
                 : *******:** ****:*:**: *******.:****:: :* :** **: 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 KNRFRHRSKAFPKREHWLDWAKEKYDERLISQIKMVTRVMFLYIPLPMFW 
                 292 
               
               
                 U13707OryctolaguscunicProteinS 
                 KNRFRHRSKQFPKRAHWLDWAKEKYDERLIAQIKMVTRVLFLYIPLPMFW 
                 294 
               
               
                 D50306RatProteinSequence 
                 KNRFRHRSKAFPKREHWLDWAKEKYDERLISQIKMVTKVMFLYIPLPMFW 
                 294 
               
               
                 NM_053079MusmusculusProteinSeq 
                 KNRFRHRSKAYPKREHWLDWAKEKYDERLISQIKMVTKVMFLFIPLPMFW 
                 294 
               
               
                 AY027496Ovis 
                 KNRISHRSKKFPKREHWLDWASEKYDERLISQIKMVTRVMFLYIPLPMFW 
                 294 
               
               
                 Caninesubmitted 
                 KNRFRHRSKQFPKREHWLDWAKEKYDERLISQIKMVTKVMFLYIPLPMFW 
                 279 
               
               
                 AY029615GallusgallusProteinSeq 
                 KNRFRHRSKEYPKREHWLDWASEKYDKRLIAQTKMVLKVLFLYIPLPMFW 
                 300 
               
               
                   
                 ***: **** :*** ******.****:***:* *** :*:**:******* 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 ALFDQQGSRWTLQATTMSGKTGALEIQPDQMQTVNAILIVIMVPIFDAVL 
                 342 
               
               
                 U13707OryctolaguscunicProteinS 
                 ALFDQQGSRWTLQATTMSGRIGILEIQPDQMQTVNTILIIILVPIMDAVV 
                 344 
               
               
                 D50306RatProteinSequence 
                 ALFDQQGSRWTLQATTMTGKIGTTEIQPDQMQTVNAILIVIMVPIVDAVV 
                 344 
               
               
                 NM_053079MusmusculusProteinSeg 
                 GLFDQQGSRWTLQATTMNGKIGANEIQPDQMQTVNAILNVNNGPNVDAVV 
                 344 
               
               
                 AY027496Ovis 
                 ALFDQQGSRWTLQATTMSGKIGIIEIQPDQMQTVNAILIVVMVPIVDAVV 
                 344 
               
               
                 Caninesubmitted 
                 ALFDQQGSRWTLQATANSGKIGLLEVQPDQMQTVNAILIVVMVPIMDAVV 
                 329 
               
               
                 AY029615GallusgallusProteinSeq 
                 ALFDQQGSRWTLQATTMDGDFGAMQIQPDQMQTVNPILIIIMVPVVDAVI 
                 350 
               
               
                   
                 .**************:* * :*  ::*********.** :   * .***: 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 YPLTAKCGFNFTSLKKMAVGMVLASMAFVVAAIVQVEIDKTLPVFPKGNE 
                 392 
               
               
                 U13707OryctolaguscunicProteinS 
                 YPLIAKCGLNFTSLKKMTIGMFLASMAFVAAAILQVEIDKTLPVFPKANE 
                 394 
               
               
                 D50306RatProteinSequence 
                 YPLIAKCGFNFTSLKKMTVGMFLASMAFVVAAIVQVEIDKTLPVFPSGNQ 
                 394 
               
               
                 NM_053079MusmusculusProteinSeq 
                 YRSTAKCGFNFTSLKKMTVGMFLASMAFVVAAIVQVWIDKTLPVEPGGNQ 
                 394 
               
               
                 AY027496Ovis 
                 YPLIAKCGLNFTSLKKMTVGMFLASMAFVAAAIVQVDIDKTLPVFPKGNE 
                 394 
               
               
                 Caninesubmitted 
                 YPLIAKCGFNFTSLKRMTVGMFLASMAFVMAAIVQLEIDKTLPVFPKQNE 
                 379 
               
               
                 AY029615GallusgallusProteinSeq 
                 YPLIQKCKINFTPLRRITVGMFLAGLAFVAAALLQVQIDKTLPVFPAAGQ 
                 400 
               
               
                   
                 *  * ** :***.*:::::**.**.:*** **::*::*********  .: 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 VQIKVLNIGNNTMNISLPG--EMVTLGPMSQTNAFMTFDVNKLTRINISS 
                 440 
               
               
                 U13707OryctolaguscunicProteinS 
                 VQIKVLNVGSENMIISLPG--QTVTLNQMSQTNEFMTFNEDTLTSINITS 
                 442 
               
               
                 D50306RatProteinSequence 
                 VQIKVLNIGNNDMAVYFPG--KNVTVAQMSQTDTFMTFDVDQLTSINVSS 
                 442 
               
               
                 NM_053079MusmusculusProteinSeq 
                 VQIKVLNIGNNNMTVHFPG--NSVTLAQMSQTDTFMTFDIDKLTSINISS 
                 442 
               
               
                 AY027496Ovis 
                 VQIKVLNIGNNSMTVSFPG--TTVTCDQMSQTNGFLTFNVDNLS-INISS 
                 441 
               
               
                 Caninesubmitted 
                 VQIKVLNIGNGAMNVSFPG--AVVTVSQMSQSDGFMTFDVDKLTSINISS 
                 427 
               
               
                 AY029615GallusgallusProteinSeq 
                 AQIKTINLGDSNANVTFLPNLQNVTVLPMESTG-YRMFESSQLKSVMVNF 
                 449 
               
               
                   
                 .***::*:*.    : :      **   *..:. :  *: . *. : :. 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 PGSP-VTAVTDDFKQGQRHTLLVWAPNHYQVVK-DGLNQKPEKGENGIRF 
                 488 
               
               
                 U13707OryctolaguscunicProteins 
                 -GSQ-VTMITPSLEAGQRHTLLVWAPNNYRVVN-DGLTQKSDKGENGIRF 
                 489 
               
               
                 D50306RatProteinSequence 
                 PGSPGVTTVAHEFEPGHRHTLLVWGPNLYRVVK-DGLNQKPEKGENGIRF 
                 491 
               
               
                 NM_053079MusmusculusProteinSeq 
                 SGSPGVTTVAHDFEQGHRHNLLVWEPSQYRVVK-DGPNQKPEKGRNGIRF 
                 491 
               
               
                 AY027496Ovis 
                 TGTP-VTPVTHNFESGHRHTLLVWAPSNYQVVK-DGLNQKPEKGRNGIRF 
                 489 
               
               
                 Caninesubmitted 
                 TGSP-VIPVTYNFEQGHRHTLLVWAPNNYRVVK-DGLNQKPEKGENGIRF 
                 475 
               
               
                 AY029615GallusgallusProteinSeq 
                 GSESRSENTDSISSNTHTVTTKNAAAGIVSSLRSDNFTSKPEEGKNLVRF 
                 499 
               
               
                   
                  .      :    .  :  .:    ..    :. *. ..*.::*.* :** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 VNTFNELTTITMSGKVYANISSYNASTYQFFPSGIKGFTISSTE-IPPQC 
                 537 
               
               
                 U13707OryctolaguscunicProteinS 
                 VNTYSQPTNVTMSGKVYEHIASYNASEYQFFTSGVKGFTVSSAG-ISEQC 
                 538 
               
               
                 D50306RatProteinSeguence 
                 VSTLNEMITTKMSGKVYENVTSHSASNYQFFPSGQKDYTINTTE-IAPNC 
                 540 
               
               
                 NM_053079MusmusculusProteinSeq 
                 VNTLNEMVTNKMSGKVYEKFTSHNASGYKFLPSGEKQYTINTTA-VAPTC 
                 540 
               
               
                 AY027496Ovis 
                 VNAFGESFGVTMDGEVYNNVSGHNASEYLFFSSGVKSFTINSPE-ISQQC 
                 538 
               
               
                 Caninesubmitted 
                 INSLNESLNITMGDKVYVNVTSHNASEYQFFSLGTKNITISSTQQISQNC 
                 525 
               
               
                 AY029615GallusgallusProteinSeq 
                 VNNLPQTVNITMGDTTFGILEETSISNYSPFSGGRTYDIVITAG--STNC 
                 547 
               
               
                   
                 :.   : .  .*.. .:  .   . * *  :. * .   : :.   .  * 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 QPNFNTFYLEFGSAYTYTVQ-RKNDSCPEVKVFEDISANTVNMALQIPQY 
                 586 
               
               
                 U13707OryctolaguscunicProteinS 
                 RRDFESPYLEFGSAYTYLIT-SQATGCPQVTEFEDIPPNTMNMAWQIPQY 
                 587 
               
               
                 D50306RatProteinSequence 
                 SSDFKSSNLDFGSAYTYVTRSRASDGCLEVKEFEDIPPNTVNMALQIPQY 
                 590 
               
               
                 NM_053079MusmusculusProteinSeq 
                 LTDFKSSNLDFGSAYTYVIR-RASDGCLEVKEFEDIPPNTVNMALQIPQY 
                 589 
               
               
                 AY027496Ovis 
                 EKQFKTSYLEFGSAFTYVIS-RKSDGCPEPKIFEDISPNTVSMALQIPQY 
                 587 
               
               
                 Caninesubmitted 
                 TKVLQSSNLEFGSAYTYVIG-TQSTGCPELHMFEDISPNTVNMALQIPQY 
                 574 
               
               
                 AY029615GallusgallusProteinSeq 
                 KP--TSEKLGYGGAYTTVTN-ECSGDCTQLRYIEDIQPNTVHMAWQIPQY 
                 594 
               
               
                   
                      :  * :*.*:* ::      .  :   :*** .**: ** ***** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 FLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAG 
                 636 
               
               
                 U13707OryctolaguscunicProteinS 
                 FLITSGEVVFSITGLEFSYSQAPSNMKSVLQDRWLLTVAVGNIIVLIVAG 
                 637 
               
               
                 D50306RatProteinSequence 
                 FLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAIGNIIVLIVAE 
                 640 
               
               
                 NM_053079MusmusculusProteinSeq 
                 FLLTCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAIGNIIVLIVAG 
                 639 
               
               
                 AY027496Ovis 
                 FLLTCGEVVFSITGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAG 
                 637 
               
               
                 Caninesubmitted 
                 FLITCGEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVACWQHHCAHCGR 
                 624 
               
               
                 AY029615GallusgallusProteinSeq 
                 FILTCCEVVFSVTGLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAG 
                 644 
               
               
                   
                 *::*.******:*******************  ******  :      . 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 AGQFSKQWAEYILFAALLLVVCVIFAIMARFYTYINPAEIEAQFDEDEKK 
                 686 
               
               
                 U13707OryctolaguscunicProteinS 
                 AGQINKQWAEYILFAALLLVVCVIFAIMARFYTYVNPAEIEAQFEEDEKK 
                 687 
               
               
                 D50306RatProteinSequence 
                 AGHFDKQWAEYVLFASLLLVVCIIFAIMARFYTYINPAEIEAQFDEDEKK 
                 690 
               
               
                 NM_053079MusmusculusProteinSeq 
                 AGHFPKQWAEYILFASLLLVVCVIFAIMARFYTYINPAEIEAQFDEDEKK 
                 689 
               
               
                 AY027496Ovis 
                 AGQFPKQWAEYVLFAALLLVVCIIFAIMARFYTYVNPAEIEAQFDEDDKE 
                 687 
               
               
                 Caninesubmitted 
                 SRPVQ-TVG-----------IHPICGIASGCLCNICHHGPVLHLRQSSRD 
                 662 
               
               
                 AY029615GallusgallusProteinSeq 
                 ASKLSEQWAEYVLFAALLFAVCIIFAVMAYFYTYTDPNEVEAQLDEEEKK 
                 694 
               
               
                   
                 :  .    .           :  * .: :             :: :..:. 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 NRLEKSNPYFMSGANSQKQM 
                 706 
               
               
                 U13707OryctolaguscunicProteinS 
                 KNPEKNDLYPSVAPVSQTQM 
                 707 
               
               
                 D50306RatProteinSequence 
                 KGVGKENPYSSLEPVSQTNM 
                 710 
               
               
                 NM_053079MusmusculusProteinSeq 
                 KGIGKENPYSSLEPVSQTQM 
                 709 
               
               
                 AY027496Ovis 
                 DDLEKSNPYAKLDFVSQTQM 
                 707 
               
               
                 Caninesubmitted 
                 -------------------- 
               
               
                 AY029615GallusgallusProteinSeq 
                 KQIKQDPDLHGKESEAVSQM 
                 714 
               
               
                   
               
             
          
           
               
                 Alignment of Amino-Acid Sequences for Canine and Human 
               
               
                   
               
             
          
           
               
                 Sequence format is Pearson 
                   
               
               
                   
               
               
                 Sequence 1: XM_007063HomosapiensProteinSeq 
                 706 aa 
               
               
                   
               
               
                 Sequence 2: Caninesubmittedclone37 
                 662 aa 
               
               
                   
               
             
          
           
               
                 Start of Pairwise alignments 
               
               
                   
               
               
                 Aligning. . .  
               
               
                   
               
               
                 Sequences (1:2) Aligned. Score: 76 
               
               
                   
               
               
                 Guide tree file created: 
               
               
                   
               
               
                 [/net/nfs0/vol1/production/w3nobody/tmp/789481.229198-238519.dnd] 
               
               
                   
               
               
                 Start of Multiple Alignment 
               
               
                   
               
               
                 There are 1 groups 
               
               
                   
               
               
                 Aligning. . .  
               
               
                   
               
               
                 Group 1: Sequences: 2 Score:12826 
               
               
                   
               
               
                 Alignment Score 3129 
               
               
                   
               
               
                 CLUSTAL-Alignment file created 
               
               
                   
               
               
                 [/net/nfs0/vol1/production/w3nobody/tmp/789481.229198-238519.aln] 
               
               
                   
               
               
                 Your Multiple Sequence Alignment: 
               
               
                   
               
               
                 789481.229198-238519.aln 
               
               
                   
               
               
                 CLUSTAL W (1.81) multiple sequence alignment 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:14) 
               
             
          
           
               
                 XM_007063HomosapiensProteinSeq 
                 MSKSHSFFGYPLSIFFIVVNEFCERFSYYGMRAILILYFTNFISWDDNLS 
                 50 
               
               
                   
               
             
          
           
               
                 (SEQ ID NO:13) 
               
             
          
           
               
                 Caninesubmittedclone37 
                 -------------IFFIVVNEFCERFSYYGMRALLILYFRRFIGWDDNLS 
                 37 
               
               
                   
                              ********************:***** .**.****** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 TAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIGQAVTSVSS 
                 100 
               
               
                 Caninesubmittedclone37 
                 TAIYHTFVALCYLTPILGALIADSWLGKFKTIVSLSIVYTIGQAVTAVSS 
                 87 
               
               
                   
                 **********************************************:*** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 INDLTDHNHDGTPDSLPVHVVLSLIGLALIALGTGGIKPCVSAFGGDQFE 
                 150 
               
               
                 Caninesubmittedclone37 
                 INDLTDYNKDGTPDNLSVHVALSMIGLALIALGTGGIKPCVSAFGGDQFE 
                 137 
               
               
                   
                 ******:*:*****.*.***.**:************************** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 EGQEKQRNRFFSIFYLAINAGSLLSTIITPMLRVQQCGIHSKQACYPLAF 
                 200 
               
               
                 Caninesubmittedclone37 
                 EGQEKQRNRFFSIFYLAINAGSLISTIVTPMLRVHECGIYSQKACYPLAF 
                 187 
               
               
                   
                 ***********************:***:******::***:*::******* 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 GVPAALMAVALIVFVLGSGMYKKFKPQGNIMGKVAKCIGFAIKNRFRHRS 
                 250 
               
               
                 Caninesubmittedclone37 
                 GVPAALMAVSLIVFVIGSGMYKKFQPQGNVMGKVVKCIGFALKNRFRHRS 
                 237 
               
               
                   
                 *********:*****:********:****:****.******:******** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 KAFPKREHWLDWAKEKYDERLISQIKMVTRVMFLYIPLPMFWALFDQQGS 
                 300 
               
               
                 Caninesubmittedclone37 
                 KQFPKREHWLDWAKEKYDERLISQIKMVTKVMFLYIPLPMFWALFDQQGS 
                 287 
               
               
                   
                 * ***************************:******************** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 RWTLQATTMSGKIGALEIQPDQMQTVNAILIVIMVPIFDAVLYPLIAKCG 
                 350 
               
               
                 Caninesubmittedclone37 
                 RWTLQATAMSGKIGLLEVQPDQMQTVNAILIVVMVPIMDAVVYPLIAKCG 
                 337 
               
               
                   
                 *******:****** **:**************:****:***:******** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 FNFTSLKKMAVGMVLASMAFVVAAIVQVEIDKTLPVFPKGNEVQIKVLNI 
                 400 
               
               
                 Caninesubmittedclone37 
                 FNFTSLKRMTVGMFLASMAFVMAAIVQLEIDKTLPVFPKQNEVQIKVLNI 
                 387 
               
               
                   
                 *******:*:***.*******:*****:*********** ********** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 GNNTMNISLPGEMVTLGPMSQTNAFMTFDVNKLTRINISSPGSPVTAVTD 
                 450 
               
               
                 Caninesubmittedclone37 
                 GNGAMNVSFPGAVVTVSQMSQSDGFMTFDVDKLTSINISSTGSPVIPVTY 
                 437 
               
               
                   
                 **.:**:*:** :**:. ***::.******:*** *****.**** .** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 DFKQGQRHTLLVWAPNHYQVVKDGLNQKPEKGENGIRFVNTFNELITITM 
                 500 
               
               
                 Caninesubmittedclone37 
                 NFEQGHRHTLLVWAPNNYRVVKDGLNQKPEKGENGIRFINSLNESLNITM 
                 487 
               
               
                   
                 :*:**:**********:*:*******************:*::** :.*** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 SGKVYANISSYNASTYQFFPSGIKGFTISST-EIPPQCQPNFNTFYLEFG 
                 549 
               
               
                 Caninesubmittedclone37 
                 GDKVYVNVTSHNASEYQFFSLGTKNITISSTQQISQNCTKVLQSSNLEFG 
                 537 
               
               
                   
                 ..***.*::*:*** ****. * *.:***** :*. :*   :::  **** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 SAYTYIVQRKNDSCPEVKVFEDISANTVNMALQIPQYFLLTCGEVVFSVT 
                 599 
               
               
                 Caninesubmittedclone37 
                 SAYTYVIGTQSTGCPELHMFEDISPNTVNMALQIPQYFLITCGEVVFSVT 
                 587 
               
               
                   
                 *****::  :. .***:::*****.**************:********** 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 GLEFSYSQAPSNMKSVLQAGWLLTVAVGNIIVLIVAGAGQFSKQWAEYIL 
                 649 
               
               
                 Caninesubmittedclone37 
                 GLEFSYSQAPSNMKSVLQAGWLLTVACWQHHCAHCGRSRPVQ-TVG---- 
                 632 
               
               
                   
                 **************************  :      . :  ..   . 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 FAALLLVVCVIFAIMARFYTYINPAEIEAQFDEDEKKNRLEKSNPYFMSG 
                 699 
               
               
                 Caninesubmittedclone37 
                 -------IHPICGIASGCLCNICHHGPVLHLRQSSRD------------- 
                 662 
               
               
                   
                        :  * .* :     *       :: :..:. 
               
               
                   
               
               
                 XM_007063HomosapiensProteinSeq 
                 ANSQKQM 
                 706 
               
               
                 Caninesubmittedclone37 
                 ------- 
               
             
          
         
       
     
     After analyzing the protein sequence and performing alignment with other species, the underlined, italicized was removed for submission to Genbank. 
     Sequence to Submit to Genbank (SEQ ID NO:7) 
     catcttcttcatcgtggtcaatgagttctgtgaaa gattttcctactatggaatgagagca ctcctgattctgtacttcagacgg ttcatcgggtgggacgataatctgtcca cggccatctaccacacgtttgtggctct gtgctacctgacgccgatcctcggc gcactgatcgcagactcctggctgggaa agttcaagacaatcgtgtcactct ccattgtctacacaattggacaggcggtc actgcagtaagctcaattaatga cctcacagactataacaaagatgg aactcctgacaatctgtccgtgcatgtggcactgt ccatgattggcctggccctgatag ctctgggaaactggaggaataaag ccctgtgtgtctgcatttggtggagaccagtttg aagagggccaggaaaaacaaag aaacagattcttttccatcttttattt ggccattaatgctggaagcttgatttccacctattg tcactcccatgctcagagttcacgaat gtggaatttacagtcagaaagcttgtt acccactggcatttggggttcctgctgct ctcatggccgtatctctgattgtattt gtcattggcagtggaatgtacaagaag tttcagccccagggtaatgtcatgggtaa agttgtcaagtgcattggttttgccct caaaaataggtttaggcaccggagtaa gcagtttcccaagagggagcactggct ggactgggctaaagagaaatacgatga gcggctcatctctcaaattaagatggt cacaaaagtgatgttcttgtacatccc actcccaatgttctgggccctgtttga ccagcagggctccaggtggacactgc aagcaacagctatgagtgggaaaattg gacttcttgaagttcagccagatc agatgcagactgtgaatgccatcttgattgtcgtcatggtccccatcatggatgccgt ggtgtaccctctgattgcaaaatgt ggcttcaatttcacctccttgaaga ggatgacagttggaatgttcctggcttccatgg ccttcgtgatggcggcgattgttca gctggaaattgataaaactcttcc agtcttccccaaacaaaatgaagtccaaatcaa agtactgaatataggaaatggtgcc atgaatgtatcttttcctggagcgg tggtgacagttagccaaatgagtcaatcagat ggatttatgacttttgatgtagaca aactgacaagtataaacatttcttcc actggatcaccagtcattccagtgacttataact ttgagcagggccatcgccatacccttct agtatgggcccccaataattaccgagt ggtaaaggatggccttaaccagaag ccagaaaaaggagaaaatggaatcaga tttataaatagtcttaatgagagcctc aacatcaccatgggcgacaaagtttat gtgaatgtcaccagtcacaatgccag cgagtatcagttcttttctttgggcac aaaaaacattacaataagttcaacacaac agatctcacaaaattgtacaaaagttct ccaatcatccaaccttgaatttggtagt gcatatacctatgtaatcggaacgcag agcactggctgccctgaattgcatatgttt gaagatatttcacccaacacagttaa catggctctgcagatcccgcagtactt cctcatcacctgcggcgaggtggtttt ctctgtcacaggactggagttct catattctcaggccccctccaacatgaagtcg gtgcttcaggcgggatggctgctgacagtggcts 
     Canine PepT1 Nucleotide Sequence (SEQ ID NO:20) 
     atgggcatgtccaagtcatatggttgc tttggttaccccttgagcatcttcttcat cgtggtcaatgagttctgtgaaagatttt cctactatggaatgagagcactcctgatt ctgtacttcagacggttcatcgggt gggacgataatctgtccacggccatcta ccacacgtttgtggctctgtgctacct gacgccgatcctcggcgcactgat cgcagactcctggctgggaaagttcaaga caatcgtgtcactctccattgtct acacaattggacaggcggtcact gcagtaagctcaattaatgacctcacagactataa caaagatggaactcctgacaatct gtccgtgcatgtggcactgtccat gattggcctggccctgatagctctgggaactgg aggaataaagccctgtgtgtctg catttggtggagaccagtttgaa gagggccaggaaaaacaaagaaacagattctttt ccatcttttatttggccattaatgctgg aagcttgatttccactattgtcactccc atgctcagagttcacgaatgtggaatttac agtcagaaagcttgttacccactggca tttggggttcctgctgctctcatggcc gtatctctgattgtatttgtcattggcagt ggaatgtacaagaagtttcagcccca gggtaatgtcatgggtaaagttgtca agtgcattggttttgccctcaaaaataggt ttaggcaccggagtaagcagtttcccaag agggagcactggctggactgggctaaa gagaaatacgatgagcggctca tctctcaaattaagatggtcacaaaagt gatgttcttgtacatcccactcccaatg ttctgggccctgtttgaccagcagggc tccaggtggacactgcaagcaacagctat gagtgggaaaattggacttcttgaagtt cagccagatcagatgcagactgt gaatgccatcttgattgtcgtcatggt cccatcatggatgccgtggtgtaccc tctGattgcaaaatgtggcttcaatttca cctccttgaagaggatgacagttggaat gttcctggcttccatggccttcgtgat ggcggcgattgttcagctggaaattga taaaactcttccagtcttccccaaac aaaatgaagtccaaatcaaagtactg aatataggaaatggtgccatgaatgtatctt ttcctggagcggtggtgacagttagcc aaatgagtcaatcagatggatttatga cttttgatgtagacaaactgacaagtat aaacatttcttccactggatcaccagt cattccagtgacttataactttgagc agggccatcgccatacccttctagtatggg cccccaataattaccgagtggtaaa ggatggccttaaccagaagccagaa aaaggagaaaatggaatcagatttataaat agtcttaatgagagcctcaacatca ccatgggcgacaaagtttatgtg aatgtcaccagtcacaatgccagcgagtatcag ttctttctttgggcacaaaaaaca ttacaataagttcaacacaacaga tctcacaaaattgtacaaaagttctccaatcatcc aaccttgaatttggtagtgcatat acctatgtaatcggaacgcagag cactggctgccctgaattgcatatgtttgaagatat ttcacccaacacagttaacat ggctctgcagatcccgcagtactt cctcatcacctgcggcgaggtggttttctctgtcaca ggactggagttatcatattctca ggccccctccaacatgaagtcggt gcttcaggcgggatggctgctgacagtggctgtt ggcaacatcattgtgctcattgtg gcaggagcaggccagttcagtgaa cagtgggctgaatacatcctatttgcggcattg cttctggttgtctgtgtaatattt gccatcatggcccggttttacactt acgtcaatccagcagagattgaagctcagtttgacg acgatgagaaaaagaacctggaaaa gatgaatgtatattccacggtaactccggtctcacagacacagatg 
     Canine PepT1 Amino Acid Sequence (SEQ ID NO:21) 
     MGMSKSYGCFGYPLSIFFIVVNEF CERFSYYGMRALLILYFRRFIGWDDNLS TAIYHTFVALCYLTPILGALIADS WLGKFKTIVSLSIVYTIGQAVTAVSSINDL TDYNKDGTPDNLSVHVALSMIG LALIALGTGGIKPCVSAFGGDQFEEGQEK QRNRFFSIFYLAINAGSLISTI VTPMLRVHECGIYSQKACYPLAFGVPAALMA VSLIVFVIGSGMYKKFQPQGN VMGKVVKCIGFALKNRFRHRSKQFPKREH WLDWAKEKYDERLISQIKMV TKVMFLYIPLPMFWALFDQQGSRWTLQATA MSGKIGLLEVQPDQMQTVNAI LIVVMVPIMDAVVYPLIAKCGFNFTSLKRM TVGMFLASMAFVMAAIVQL EIDKTLPVFPKQNEVQIK VLNIGNGAMNVSFP GAVVTVSQMSQSDGFMIFDVDKLTS INISSTGSPVIPVTYNFEQGHRHTLLV WAPNNYRVVKDGLNQKPEKGENG IRFINSLNESLNITMGDKVYVNVTSHN ASEYQFFSLGTKNITISSTQQIS QNCTKVLQSSNLEFGSAYTYVIGTQSTGCPE LHMFEDISPNTVNMALQIPQYFLI TCGEVVFSVTGLEFSYSQAPSNMKSVLQ AGWLLTVAVGNIIVLIVAGAGQF SEQWAEYILFAALLLVVCVIFAIMARFYT YVNPAEIEAQFDDDEKKNLEKMNVYSTVTPVSQTQM 
     All publications, patents and patent documents are incorporated by reference herein, as though individually incorporated by reference. The invention has been described with reference to various specific and preferred embodiments and techniques. However, it should be understood that many variations and modifications may be made while remaining within the scope of the invention. 
     
       
         
               
             
           
               
                   
               
             
             
               
                                    
               
               
                 #             SEQUENCE LISTING  
               
               
                   
               
               
                   
               
               
                 &lt;160&gt; NUMBER OF SEQ ID NOS: 21  
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 1  
               
               
                 &lt;211&gt; LENGTH: 3045  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Homo sapiens  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 1  
               
               
                   
               
               
                 gaatgtccaa atcacacagt ttctttggtt atcccctgag catcttcttc at  
               
               
                 #cgtggtca     60  
               
               
                   
               
               
                 atgagttttg cgaaagattt tcctactatg gaatgcgagc aatcctgatt ct  
               
               
                 #gtacttca    120  
               
               
                   
               
               
                 caaatttcat cagctgggat gataacctgt ccaccgccat ctaccatacg tt  
               
               
                 #tgtggctc    180  
               
               
                   
               
               
                 tgtgctacct gacgccaatt ctcggagctc ttatcgccga ctcgtggctg gg  
               
               
                 #aaagttca    240  
               
               
                   
               
               
                 agaccattgt gtcgctctcc attgtctaca caattggaca agcagtcacc tc  
               
               
                 #agtaagct    300  
               
               
                   
               
               
                 ccattaatga cctcacagac cacaaccatg atggcacccc cgacagcctt cc  
               
               
                 #tgtgcacg    360  
               
               
                   
               
               
                 tggtgctgtc cttgatcggc ctggccctga tagctctcgg gactggagga at  
               
               
                 #caaaccct    420  
               
               
                   
               
               
                 gtgtgtctgc gtttggtgga gatcagtttg aagagggcca ggagaaacaa ag  
               
               
                 #aaacagat    480  
               
               
                   
               
               
                 ttttttccat cttttacttg gctattaatg ctggaagttt gctttccaca at  
               
               
                 #catcacac    540  
               
               
                   
               
               
                 ccatgctcag agttcaacaa tgtggaattc acagtaaaca agcttgttac cc  
               
               
                 #actggcct    600  
               
               
                   
               
               
                 ttggggttcc tgctgctctc atggctgtag ccctgattgt gtttgtcctt gg  
               
               
                 #cagtggga    660  
               
               
                   
               
               
                 tgtacaagaa gttcaagcca cagggcaaca tcatgggtaa agtggccaag tg  
               
               
                 #catcggtt    720  
               
               
                   
               
               
                 ttgccatcaa aaatagattt aggcatcgga gtaaggcatt tcccaagagg ga  
               
               
                 #gcactggc    780  
               
               
                   
               
               
                 tggactgggc taaagagaaa tacgatgagc ggctcatctc ccaaattaag at  
               
               
                 #ggttacga    840  
               
               
                   
               
               
                 gggtgatgtt cctgtatatt ccactcccaa tgttctgggc cttgtttgac ca  
               
               
                 #gcagggct    900  
               
               
                   
               
               
                 ccaggtggac actgcaggca acaactatgt ccgggaaaat cggagctctt ga  
               
               
                 #aattcagc    960  
               
               
                   
               
               
                 ccgatcagat gcagaccgtg aacgccatcc tgatcgtgat catggtcccg at  
               
               
                 #cttcgatg   1020  
               
               
                   
               
               
                 ctgtgctgta ccctctcatt gcaaaatgtg gcttcaattt cacctccttg aa  
               
               
                 #gaagatgg   1080  
               
               
                   
               
               
                 cagttggcat ggtcctggcc tccatggcct ttgtggtggc tgccatcgtg ca  
               
               
                 #ggtggaaa   1140  
               
               
                   
               
               
                 tcgataaaac tcttccagtc ttccccaaag gaaacgaagt ccaaattaaa gt  
               
               
                 #tttgaata   1200  
               
               
                   
               
               
                 taggaaacaa taccatgaat atatctcttc ctggagagat ggtgacactt gg  
               
               
                 #cccaatgt   1260  
               
               
                   
               
               
                 ctcaaacaaa tgcatttatg acttttgatg taaacaaact gacaaggata aa  
               
               
                 #catttctt   1320  
               
               
                   
               
               
                 ctcctggatc accagtcact gctgtaactg acgacttcaa gcagggccaa cg  
               
               
                 #ccacacgc   1380  
               
               
                   
               
               
                 ttctagtgtg ggcccccaat cactaccagg tggtaaagga tggtcttaac ca  
               
               
                 #gaagccag   1440  
               
               
                   
               
               
                 aaaaagggga aaatggaatc agatttgtaa atacttttaa cgagctcatc ac  
               
               
                 #catcacaa   1500  
               
               
                   
               
               
                 tgagtgggaa agtttatgca aacatcagca gctacaatgc cagcacatac ca  
               
               
                 #gttttttc   1560  
               
               
                   
               
               
                 cttctggcat aaaaggcttc acaataagct caacagagat tccgccacaa tg  
               
               
                 #tcaaccta   1620  
               
               
                   
               
               
                 atttcaatac tttctacctt gaatttggta gtgcttatac ctatatagtc ca  
               
               
                 #aaggaaga   1680  
               
               
                   
               
               
                 atgacagctg ccctgaagtg aaggtgtttg aagatatttc agccaacaca gt  
               
               
                 #taacatgg   1740  
               
               
                   
               
               
                 ctctgcaaat cccgcagtat tttcttctca cctgtggcga agtggtcttc tc  
               
               
                 #tgtcacgg   1800  
               
               
                   
               
               
                 gattggaatt ctcatattct caggctcctt ccaacatgaa gtcggtgctt ca  
               
               
                 #ggcaggat   1860  
               
               
                   
               
               
                 ggctgctgac cgtggctgtt ggcaacatca ttgtgctcat cgtggcaggg gc  
               
               
                 #aggccagt   1920  
               
               
                   
               
               
                 tcagcaaaca gtgggccgag tacattctat ttgccgcgtt gcttctggtc gt  
               
               
                 #ctgtgtaa   1980  
               
               
                   
               
               
                 tttttgccat catggctcgg ttctatactt acatcaaccc agcggagatc ga  
               
               
                 #agctcaat   2040  
               
               
                   
               
               
                 ttgatgagga tgaaaagaaa aacagactgg aaaagagtaa cccatatttc at  
               
               
                 #gtcagggg   2100  
               
               
                   
               
               
                 ccaattcaca gaaacagatg tgaaggtcag gaggcaagtg gaggatggac tg  
               
               
                 #ggcccgca   2160  
               
               
                   
               
               
                 gatgccctga cctctgcccc caggtagcag gacactccat tggatggccc ct  
               
               
                 #gatgagga   2220  
               
               
                   
               
               
                 agacttcaga attgggaact aaaccatgaa tgctattttc ttttttcttt tt  
               
               
                 #cttttctt   2280  
               
               
                   
               
               
                 tttttttttt tttttttttt tgagacagag ttttgctctt gttgtccagg ct  
               
               
                 #ggagtgca   2340  
               
               
                   
               
               
                 atggcacgat ctcagctcac tgcaacctcc gcctcccagg ttcaagtaat tc  
               
               
                 #tcctgcct   2400  
               
               
                   
               
               
                 cagcctcccg agtggctggg attagcggca tgcaccacca cgcccagcta tt  
               
               
                 #tttgtatt   2460  
               
               
                   
               
               
                 tttagtagag atggggtttc accatgttgg ccaggatggt ctcgatctct tg  
               
               
                 #acctggtg   2520  
               
               
                   
               
               
                 atctgcccac ctcggcctgc caaagtgctg ggattacagg cttgagctac cg  
               
               
                 #cgcccggc   2580  
               
               
                   
               
               
                 cgtgaacgct attttctaag cagccagcag tgaatctaaa actctggaag aa  
               
               
                 #gtcttctg   2640  
               
               
                   
               
               
                 tttgaaaggc ttatttaagc cacacgtaca cacactgtct tagagtactg tg  
               
               
                 #agcccacc   2700  
               
               
                   
               
               
                 ccacattggt catcttccct atcacacaaa tgatgttatt ttggactagc tt  
               
               
                 #aattttga   2760  
               
               
                   
               
               
                 aatggtaaca aagtttccta ttccatactg ttcatttcta atactcttac ga  
               
               
                 #aaactatt   2820  
               
               
                   
               
               
                 ctaaaggagg caggagccaa ggccaaaagt gaacgtacag gtttgaaatg gc  
               
               
                 #tgtgataa   2880  
               
               
                   
               
               
                 ggaccagctg gtattaactg ataactttac ctttgggttt ttgttatttt gt  
               
               
                 #ttttctag   2940  
               
               
                   
               
               
                 tccctacctg tgtttaaatt atggataact cgaaagacag ctcaggtgaa gg  
               
               
                 #ccagtaat   3000  
               
               
                   
               
               
                 gatttttttg aagtttcaat ggtgtgaaat aaatttctgt tctta    
               
               
                 #                3045  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 2  
               
               
                 &lt;211&gt; LENGTH: 2829  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Ovis aries  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 2  
               
               
                   
               
               
                 gaaacaacat ctttagcacg gattcctccc acctggactc ctcgctcgcc ag  
               
               
                 #tcgcaggg     60  
               
               
                   
               
               
                 agccctcgga gccgccagca tgggaatgtc cgtgccgaag agctgcttcg gt  
               
               
                 #tacccctt    120  
               
               
                   
               
               
                 aagcatcttc ttcatcgtgg tcaatgagtt ctgcgaaagg ttctcttact at  
               
               
                 #ggaatgag    180  
               
               
                   
               
               
                 agcactcctg atcctgtact tccaacgttt cctgggctgg aacgacaacc tg  
               
               
                 #ggcaccgc    240  
               
               
                   
               
               
                 catctatcac acgttcgtcg ccctgtgcta cctgacgccc atcctcggag ct  
               
               
                 #ctcatcgc    300  
               
               
                   
               
               
                 cgactcctgg ctggggaagt tcaagacgat cgtgtcgctg tccatcgtct ac  
               
               
                 #accattgg    360  
               
               
                   
               
               
                 gcaggtagtc atcgctgtga gctcaattaa tgacctcact gacttcaacc at  
               
               
                 #gatggaac    420  
               
               
                   
               
               
                 cccaaacaat atttctgtgc acgtggcact ctccatgatt ggcctggtcc tg  
               
               
                 #atagctct    480  
               
               
                   
               
               
                 gggtaccgga gggataaagc cttgcgtgtc tgcatttggc ggagatcagt tt  
               
               
                 #gaagaggg    540  
               
               
                   
               
               
                 ccaggaaaag caaaggaaca gatttttttc catcttttat ttggccatta at  
               
               
                 #gctggaag    600  
               
               
                   
               
               
                 tttgctttct actatcatca cccccatgct cagagttcag gtatgcggaa tt  
               
               
                 #cacagtaa    660  
               
               
                   
               
               
                 gcaagcttgt taccccctgg cctttggggt tcctgctgca ctcatggctg ta  
               
               
                 #tctctgat    720  
               
               
                   
               
               
                 cgtgtttgtc attggcagtg gaatgtacaa gaaggtccag ccccagggta ac  
               
               
                 #atcatgtc    780  
               
               
                   
               
               
                 taaagttgcc aggtgcattg ggtttgccat caaaaatagg attagccatc gg  
               
               
                 #agtaagaa    840  
               
               
                   
               
               
                 atttcctaag agggagcact ggctggactg ggctagcgag aaatatgatg ag  
               
               
                 #cggctcat    900  
               
               
                   
               
               
                 ctctcaaatt aagatggtta caagggtgat gttcctgtac attcctctcc cc  
               
               
                 #atgttctg    960  
               
               
                   
               
               
                 ggccttgttt gatcagcagg gctccaggtg gacactgcaa gcaacgacca tg  
               
               
                 #agtgggaa   1020  
               
               
                   
               
               
                 gattggaatc attgaaatcc agccggatca gatgcagacg gtgaacgcca tc  
               
               
                 #ctgatcgt   1080  
               
               
                   
               
               
                 cgtcatggtc cccatcgtgg atgccgtggt atatcctctg atcgcaaagt gt  
               
               
                 #ggtttaaa   1140  
               
               
                   
               
               
                 tttcacctcc ctgaagaaga tgaccgtcgg catgtttctg gcctccatgg ct  
               
               
                 #ttcgtggc   1200  
               
               
                   
               
               
                 agctgccatc gtgcaggtgg acattgacaa aactctgccc gtcttcccca aa  
               
               
                 #ggaaatga   1260  
               
               
                   
               
               
                 agtccaaatc aaagtcctga atataggaaa taatagcatg accgtgtctt tt  
               
               
                 #cccggaac   1320  
               
               
                   
               
               
                 gacagtgaca tgtgaccaga tgtctcaaac aaacggattt ctgactttca ac  
               
               
                 #gtagacaa   1380  
               
               
                   
               
               
                 cctaagtata aacatttctt ctactggaac accagtcact ccagtaactc at  
               
               
                 #aactttga   1440  
               
               
                   
               
               
                 gtccggccat cgccataccc ttctcgtctg ggccccaagt aactaccaag tg  
               
               
                 #gtaaaaga   1500  
               
               
                   
               
               
                 tggccttaac cagaagccag aaaaagggag aaatggaatc agattcgtta at  
               
               
                 #gcttttgg   1560  
               
               
                   
               
               
                 cgagagcttc ggcgtcacaa tggatgggga agtttacaac aatgtctccg gt  
               
               
                 #cacaatgc   1620  
               
               
                   
               
               
                 cagtgaatat ctttttttct cttctggcgt aaagagcttc acaataaact ca  
               
               
                 #ccagagat   1680  
               
               
                   
               
               
                 ttcacaacag tgtgaaaaac agttcaaaac atcctacctt gaatttggta gt  
               
               
                 #gcgtttac   1740  
               
               
                   
               
               
                 ctatgtaatc agcagaaaga gtgacggttg ccccgaacca aagattttcg aa  
               
               
                 #gacatctc   1800  
               
               
                   
               
               
                 ccccaacaca gtcagcatgg ctctgcagat cccccagtac ttcctcctca cc  
               
               
                 #tgtggcga   1860  
               
               
                   
               
               
                 ggtggtcttc tccatcaccg gcctggagtt ctcctattct caggctcctt cc  
               
               
                 #aacatgaa   1920  
               
               
                   
               
               
                 gtcggtactt caggcaggat ggctgttgac cgtggccgtt ggcaacatca tc  
               
               
                 #gtgcttat   1980  
               
               
                   
               
               
                 tgtggcagga gcaggccagt tcagtgaaca gtgggccgag tacgttctgt tt  
               
               
                 #gcggcatt   2040  
               
               
                   
               
               
                 gcttctggtc gtctgcataa tatttgccat catggctcga ttctatacgt at  
               
               
                 #gtcaaccc   2100  
               
               
                   
               
               
                 cgcagagatt gaagctcagt ttgatgagga tgacaaggag gatgacctgg aa  
               
               
                 #aagagtaa   2160  
               
               
                   
               
               
                 cccatacgcc aagctggact tcgtctcaca gacacaaatg tgaatgtcag ga  
               
               
                 #agcaagcg   2220  
               
               
                   
               
               
                 gacgcggggc tgggccaggg tgtgcccagg ggtctgtccc atgggggcag ga  
               
               
                 #cactctgt   2280  
               
               
                   
               
               
                 tgggtggcct ctgatgggga agacttcaga actgtggacc aaaccaagac ag  
               
               
                 #ctgctttc   2340  
               
               
                   
               
               
                 tcagcagccg gcaatgaacc tgaaactcca aaagacgtcc ttttgtttgt tt  
               
               
                 #gtttttag   2400  
               
               
                   
               
               
                 agaagtctta tttaaagcgc acacacacgc acacgcacac acatgcacac ac  
               
               
                 #acacactt   2460  
               
               
                   
               
               
                 ttataagagt ccatactctg cctgaactcc ttttcctaac acacaaataa ag  
               
               
                 #ttattttg   2520  
               
               
                   
               
               
                 gactaacttg aatttttgaa atggtggcca agctccatac gtgcattcgc ac  
               
               
                 #actctgtg   2580  
               
               
                   
               
               
                 caaacaatgt taaaggaggc aaaaagtgaa tggttggggc ttttgaatag ta  
               
               
                 #cgtgttca   2640  
               
               
                   
               
               
                 taataaggac cggctggtat taactgataa ctctaccttc tgtttttagt tc  
               
               
                 #tgtttttc   2700  
               
               
                   
               
               
                 cattccctac ctctttgtaa attatggatt aacctttgaa aaaccactca gg  
               
               
                 #taaaggca   2760  
               
               
                   
               
               
                 agtcatgatt tttggagtct caacggtatg aaataaactc tcattctcaa ga  
               
               
                 #aaaaaaaa   2820  
               
               
                   
               
               
                 aaaaaaaaa                 
               
               
                 #                   
               
               
                 #                   
               
               
                 #       2829  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 3  
               
               
                 &lt;211&gt; LENGTH: 2900  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Rattus norvegicus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 3  
               
               
                   
               
               
                 ctgaactcct gcttgccagt cgccggtcag gagcctcgga gccgccacaa tg  
               
               
                 #gggatgtc     60  
               
               
                   
               
               
                 caagtctcgg ggttgctttg gctacccatt gagcatcttc ttcatcgtgg tc  
               
               
                 #aatgaatt    120  
               
               
                   
               
               
                 ctgtgaaaga ttctcctact atgggatgcg agctctcctg gttctgtact tc  
               
               
                 #aggaactt    180  
               
               
                   
               
               
                 ccttggctgg gatgatgacc tctccacggc catctaccat acgtttgttg cc  
               
               
                 #ctctgcta    240  
               
               
                   
               
               
                 cctgactcca attcttggag ctctgatcgc agactcgtgg ctggggaagt tc  
               
               
                 #aagacaat    300  
               
               
                   
               
               
                 tgtctcacta tccatcgtct acacgatcgg acaggccgtc atctcagtga gc  
               
               
                 #tcaattaa    360  
               
               
                   
               
               
                 tgaccttaca gaccatgacc acgacggcag tcctaacaac cttcctttgc ac  
               
               
                 #gtagcact    420  
               
               
                   
               
               
                 gtccatgatc ggcctggccc tgatagccct tggtacagga ggaatcaagc cc  
               
               
                 #tgtgtgtc    480  
               
               
                   
               
               
                 tgcatttggt ggcgatcagt ttgaagaggg tcaggaaaaa cagcgaaacc gg  
               
               
                 #ttcttttc    540  
               
               
                   
               
               
                 catcttttat ttggctatca acgcaggaag cctgctctcc acgatcatca ct  
               
               
                 #cccatact    600  
               
               
                   
               
               
                 cagagttcag cagtgcggaa tccacagcca acaagcttgt tacccactgg cc  
               
               
                 #tttggggt    660  
               
               
                   
               
               
                 tccggcagct ctcatggctg ttgccctaat tgtgtttgtc ctcggcagtg ga  
               
               
                 #atgtacaa    720  
               
               
                   
               
               
                 gaagtttcag ccccagggca acatcatggg caaagtggcc aagtgcattg gc  
               
               
                 #tttgccat    780  
               
               
                   
               
               
                 caaaaacagg tttcggcacc gaagtaaggc atttcccaag agggaacact gg  
               
               
                 #ctggactg    840  
               
               
                   
               
               
                 ggctaaagag aaatacgatg agaggctcat ctcgcagatt aagatggtga cg  
               
               
                 #aaggtgat    900  
               
               
                   
               
               
                 gttcctgtac attcccctcc ccatgttttg ggccttgttt gaccagcagg gt  
               
               
                 #tccaggtg    960  
               
               
                   
               
               
                 gacactgcaa gcaacgacca tgactgggaa aattggaaca attgagattc ag  
               
               
                 #ccggacca   1020  
               
               
                   
               
               
                 gatgcagacg gtgaacgcca tcttgattgt catcatggtc cccattgtgg ac  
               
               
                 #gccgtggt   1080  
               
               
                   
               
               
                 gtatccgctc attgcaaaat gtggtttcaa cttcacctcc ctgaagaaga tg  
               
               
                 #accgttgg   1140  
               
               
                   
               
               
                 gatgttcctg gcatccatgg cctttgtggt ggctgcaatt gtgcaggtgg aa  
               
               
                 #atcgataa   1200  
               
               
                   
               
               
                 aactcttcca gtcttcccca gcggaaatca agttcaaatt aaggtcttga ac  
               
               
                 #attggaaa   1260  
               
               
                   
               
               
                 caatgacatg gccgtgtatt ttcctggaaa gaatgtgaca gttgcccaaa tg  
               
               
                 #tctcagac   1320  
               
               
                   
               
               
                 agacacattc atgactttcg atgtagacca gctgacaagc ataaacgtgt ct  
               
               
                 #tctcccgg   1380  
               
               
                   
               
               
                 atctccaggc gtcaccacgg tagctcatga gtttgagccg ggtcaccggc ac  
               
               
                 #acccttct   1440  
               
               
                   
               
               
                 agtgtggggc cccaatctat accgtgtggt aaaagacggt cttaaccaaa ag  
               
               
                 #ccagagaa   1500  
               
               
                   
               
               
                 aggggagaac ggaatcagat tcgtcagcac ccttaacgag atgatcacca tc  
               
               
                 #aaaatgag   1560  
               
               
                   
               
               
                 tggaaaagtg tacgaaaatg tcaccagtca cagcgccagc aactatcagt tt  
               
               
                 #ttcccttc   1620  
               
               
                   
               
               
                 tggccaaaaa gactacacaa taaacaccac agagattgca ccaaactgtt ca  
               
               
                 #tctgattt   1680  
               
               
                   
               
               
                 taaatcttcc aaccttgact tcggcagcgc gtacacctac gtgatcagaa gt  
               
               
                 #agggcgag   1740  
               
               
                   
               
               
                 tgatggctgc ctggaagtga aggaattcga agacatccca cccaacacgg tg  
               
               
                 #aacatggc   1800  
               
               
                   
               
               
                 cctgcagatc ccacagtact tcctcctcac ctgcggcgag gtggtcttct ct  
               
               
                 #gtcacagg   1860  
               
               
                   
               
               
                 actggagttc tcctattccc aggccccgtc taacatgaag tccgtgcttc ag  
               
               
                 #gcaggatg   1920  
               
               
                   
               
               
                 gcttctaacc gtggccatcg gtaatatcat tgtcctcatt gtggctgagg ca  
               
               
                 #ggccactt   1980  
               
               
                   
               
               
                 cgacaaacag tgggctgagt atgttctgtt cgcctccttg ctcctggtcg tc  
               
               
                 #tgcatcat   2040  
               
               
                   
               
               
                 atttgccatt atggcccgat tctacaccta catcaaccca gcagagatcg ag  
               
               
                 #gcacagtt   2100  
               
               
                   
               
               
                 cgatgaggat gagaagaaaa agggcgtagg gaaggaaaac ccgtattcct cg  
               
               
                 #ttggaacc   2160  
               
               
                   
               
               
                 tgtctcacag acaaacatgt gaagatcaga aagcaagtgg agaacatacc aa  
               
               
                 #gtccagca   2220  
               
               
                   
               
               
                 ttcaccatga cctctgccca agggacagga ccctccacca cagagtcctt gc  
               
               
                 #tggagaaa   2280  
               
               
                   
               
               
                 gacttcagac atgtgagcca aaataataac aaagcaggtt ttcaggctga cg  
               
               
                 #gctgtgaa   2340  
               
               
                   
               
               
                 tctgaaactc taggggagcc tttttaattt gtttttcttg agacaaggta tc  
               
               
                 #tctgtgta   2400  
               
               
                   
               
               
                 accctggcta tcctggaact cactctatag accaggctgg cctcgaactc ac  
               
               
                 #agatatct   2460  
               
               
                   
               
               
                 gtctgcctct gcctcctaag tactgggatt caaggcatgt acggcaactg cc  
               
               
                 #cagctaaa   2520  
               
               
                   
               
               
                 atattattta taacatgcac tttctgggtt ttttgttttt aaaacatact tt  
               
               
                 #ttttttta   2580  
               
               
                   
               
               
                 acactgggcc atttctaaca tttctgccac agaagtggat ttagctcaga tt  
               
               
                 #aattttga   2640  
               
               
                   
               
               
                 aaaggtaaca gtactgtttt ttttccttaa tgctcttatg aaaacaatgt tg  
               
               
                 #aatttaca   2700  
               
               
                   
               
               
                 gagggctttt ttagcagtgt gtagtgagtg tcagctgatt cgagctaata ac  
               
               
                 #cttacctc   2760  
               
               
                   
               
               
                 ggggtttttg tttctttgtt ttcctggtct cctttgcctg acctcttttt aa  
               
               
                 #attatgtg   2820  
               
               
                   
               
               
                 taattcaaaa gactattcaa gtgatggtta gtcatgagtc gtgacgtttg ac  
               
               
                 #tggtgtga   2880  
               
               
                   
               
               
                 agtaaattct tgttcttaag             
               
               
                 #                   
               
               
                 #                 290  
               
               
                 #0  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 4  
               
               
                 &lt;211&gt; LENGTH: 3128  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Mus musculus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 4  
               
               
                   
               
               
                 gtcgcccgtc cggagccttg gagccaccac aatggggatg tccaagtctc gg  
               
               
                 #ggttgctt     60  
               
               
                   
               
               
                 cggttacccg ttgagcatct tcttcatcgt ggtcaatgaa ttctgtgaaa ga  
               
               
                 #ttctccta    120  
               
               
                   
               
               
                 ctatggcatg cgagcactcc tggttctgta cttcaggaac ttcctcggct gg  
               
               
                 #gacgacaa    180  
               
               
                   
               
               
                 tctctccacg gccatttacc atacgttcgt tgccctctgc tacctgactc ca  
               
               
                 #attcttgg    240  
               
               
                   
               
               
                 agctctgatc gcagactcgt ggctggggaa gttcaagaca attgtttcac ta  
               
               
                 #tccatcgt    300  
               
               
                   
               
               
                 ctacacgatt ggacaagcag tcatctcggt gagctcaatt aatgacctca ca  
               
               
                 #gaccacga    360  
               
               
                   
               
               
                 ccacaatggc agtcctgaca gccttcccgt gcacgtagca ctgtccatgg tt  
               
               
                 #ggcctggc    420  
               
               
                   
               
               
                 cctgatagcc cttggtacag gaggaatcaa gccctgtgtg tctgcgtttg gt  
               
               
                 #ggcgatca    480  
               
               
                   
               
               
                 gtttgaagag ggtcaggaaa aacagcgaaa ccggttcttt tccatctttt at  
               
               
                 #ttggctat    540  
               
               
                   
               
               
                 caacggggga agcctgctct ccacgatcat cactcccata ctcagagttc aa  
               
               
                 #cagtgcgg    600  
               
               
                   
               
               
                 aatccacagt caacaagctt gttacccact ggccttcggg gttccagcgg ct  
               
               
                 #ctcatggc    660  
               
               
                   
               
               
                 tgttgcccta attgtgtttg tccttggcag tggaatgtac aagaagttcc ag  
               
               
                 #ccccaggg    720  
               
               
                   
               
               
                 caacatcatg ggcaaagtgg ccaagtgcat tggttttgcc atcaaaaaca gg  
               
               
                 #tttcggca    780  
               
               
                   
               
               
                 ccgaagtaag gcatatccca agagggagca ctggctggac tgggctaaag ag  
               
               
                 #aaatacga    840  
               
               
                   
               
               
                 cgagcggctc atctcacaga ttaagatggt cacgaaggtg atgttcctgt tc  
               
               
                 #atcccact    900  
               
               
                   
               
               
                 ccccatgttc tggggcctgt ttgaccaaca agggtccaga tggacactgc aa  
               
               
                 #gcaacgac    960  
               
               
                   
               
               
                 catgaatggg aaaattggag caaatgaaat tcagccggac cagatgcaga cg  
               
               
                 #gtgaatgc   1020  
               
               
                   
               
               
                 catcctgaat gtcaacaatg gccccaatgt ggacgccgtt gtgtaccgct ca  
               
               
                 #attgcaaa   1080  
               
               
                   
               
               
                 atgtggtttc aacttcacat ccctgaagaa gatgactgtt gggatgttcc tg  
               
               
                 #gcgtccat   1140  
               
               
                   
               
               
                 ggcctttgtg gtggctgcaa ttgtgcaggt ggaaatcgat aaaactcttc ca  
               
               
                 #gtcttccc   1200  
               
               
                   
               
               
                 tggtggaaat caagtccaaa ttaaggtctt gaacatcgga aacaataaca tg  
               
               
                 #accgtgca   1260  
               
               
                   
               
               
                 ttttcctgga aatagtgtga cgcttgccca aatgtctcag acagacacgt tc  
               
               
                 #atgacttt   1320  
               
               
                   
               
               
                 cgatatagac aagctgacaa gcataaacat atcttcctct ggatccccag ga  
               
               
                 #gtcaccac   1380  
               
               
                   
               
               
                 agtagctcat gattttgagc agggtcaccg gcacaacctt ctagtgtggg aa  
               
               
                 #cccagtca   1440  
               
               
                   
               
               
                 ataccgtgtg gtaaaagatg gtcctaacca aaagccagag aaaggggaga ac  
               
               
                 #ggaatcag   1500  
               
               
                   
               
               
                 gtttgtcaac acccttaacg agatggtcac caacaaaatg agtgggaaag ta  
               
               
                 #tatgaaaa   1560  
               
               
                   
               
               
                 attcacaagt cacaacgcca gcggctacaa gttcctccct tctggcgaaa ag  
               
               
                 #cagtacac   1620  
               
               
                   
               
               
                 aataaacacc acggcggtgg caccaacctg tctaactgat tttaaatctt cc  
               
               
                 #aaccttga   1680  
               
               
                   
               
               
                 ctttggcagc gcgtatacct acgtgatccg aagggcgagt gatggctgcc tg  
               
               
                 #gaagtgaa   1740  
               
               
                   
               
               
                 ggaatttgaa gacatcccac ccaacactgt gaacatggct ctgcagatcc ca  
               
               
                 #cagtactt   1800  
               
               
                   
               
               
                 ccttctcacc tgcggcgagg tggtcttctc tgtcacagga ctggagttct ct  
               
               
                 #tattccca   1860  
               
               
                   
               
               
                 ggctccgtct aacatgaagt ccgtgcttca ggcaggctgg cttctaactg tg  
               
               
                 #gcggtcgg   1920  
               
               
                   
               
               
                 caatatcatt gtgctcatcg tggcaggggc ggggcacttc cccaaacagt gg  
               
               
                 #gctgagta   1980  
               
               
                   
               
               
                 cattctgttt gcctcattgc ttctggttgt ctgcgtgata ttcgccatca tg  
               
               
                 #gctcgatt   2040  
               
               
                   
               
               
                 ctacacctac atcaacccag cagagattga agcacagttt gatgaggatg ag  
               
               
                 #aagaaaaa   2100  
               
               
                   
               
               
                 gggcatagga aaggaaaacc cgtattcttc attggaacca gtctcacaga ca  
               
               
                 #aatatgtg   2160  
               
               
                   
               
               
                 aagggcagaa ggcaaattgg agaaagatca agttcaacat gagccctgac ct  
               
               
                 #ctgtccaa   2220  
               
               
                   
               
               
                 gggacaggac actccaccac agagtccctg atggagaaag acctcagaag tg  
               
               
                 #tgagccag   2280  
               
               
                   
               
               
                 aataataaca aagcaggttt tctaaccaac agctgtgaac ctgaaactct ag  
               
               
                 #gggagcct   2340  
               
               
                   
               
               
                 tttttattta aaaaaatttt ttttttaatt ttttaaattt tttttatttt tt  
               
               
                 #attttttt   2400  
               
               
                   
               
               
                 tgcttgtttg tttgtttcga gacagggttt ctcgtgtgta gcccttggtt gt  
               
               
                 #cctggaac   2460  
               
               
                   
               
               
                 tcactctgta gaccagactg gcctcaaact cagaaatcca cctgcccctg cc  
               
               
                 #cctgcccc   2520  
               
               
                   
               
               
                 tgcccctgcc cctgcccctg cctctgcctc tgcctcccaa gtgctggatt tg  
               
               
                 #gaggcatg   2580  
               
               
                   
               
               
                 caccaccatg cccagctata attttttttt tttaagacag ggattctctg ta  
               
               
                 #taagcctg   2640  
               
               
                   
               
               
                 actgccctgg aacttgctct atagaccagg ctggccttga actcacagag at  
               
               
                 #ctgcctgc   2700  
               
               
                   
               
               
                 ctcttcctcc taagtactgg gatttcaggc atgcaccaca actgcccagc ta  
               
               
                 #aaatatta   2760  
               
               
                   
               
               
                 tttataatat gcactttctg gtttgttttt gtttttcttt taaactgggc tg  
               
               
                 #tatcttac   2820  
               
               
                   
               
               
                 atttctgcca cagaaatgaa cttagctcag attaacttaa ttttgaaaag gc  
               
               
                 #aatagtat   2880  
               
               
                   
               
               
                 tgttttttct aacagtttta tgaaaacaat attgaattta cagagggctt tt  
               
               
                 #ttaatagt   2940  
               
               
                   
               
               
                 gtgtaatgag tatcaactga ttcaagctaa ttgctttacc ttggggtttt tt  
               
               
                 #tgtttgtt   3000  
               
               
                   
               
               
                 tgtttgtttg tttgtttgtt tgtttttcta gtctcctttg ccttacctct tt  
               
               
                 #ttaaatta   3060  
               
               
                   
               
               
                 tgtgtaattc aaaagactag tcatgagttg tgaagtttca ctggtctgaa at  
               
               
                 #aaattcta   3120  
               
               
                   
               
               
                 gttcttaa                 
               
               
                 #                   
               
               
                 #                   
               
               
                 #        3128  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 5  
               
               
                 &lt;211&gt; LENGTH: 2709  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Oryctolagus cuniculus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 5  
               
               
                   
               
               
                 caccatggga atgtctaagt cactgagctg cttcggctat cccctgagca tc  
               
               
                 #ttcttcat     60  
               
               
                   
               
               
                 cgtggtcaat gagttctgcg aaaggttctc ctactatggg atgagagcac tc  
               
               
                 #ctgattct    120  
               
               
                   
               
               
                 gtacttcaga aacttcatcg gctgggacga caacctgtcc acggtcatct ac  
               
               
                 #cacacgtt    180  
               
               
                   
               
               
                 cgtcgcgctg tgctacctca cgcccattct cggagctctc atcgccgacg cg  
               
               
                 #tggctggg    240  
               
               
                   
               
               
                 gaagttcaag accatcgtgt ggctgtccat cgtctacacc atcggacaag ca  
               
               
                 #gtcacctc    300  
               
               
                   
               
               
                 cctcagctcc gtcaatgagc tcacagacaa caaccatgac gggacccccg ac  
               
               
                 #agcctccc    360  
               
               
                   
               
               
                 tgtgcacgtg gcggtgtgca tgatcggcct gctcctgata gccctcggga ca  
               
               
                 #ggaggaat    420  
               
               
                   
               
               
                 caagccctgt gtgtctgcct ttggcggcga tcagtttgag gagggccagg aa  
               
               
                 #aagcaaag    480  
               
               
                   
               
               
                 aaaccggttt ttttccatct tttacttggc cattaacgct gggagtctgc tg  
               
               
                 #tccacaat    540  
               
               
                   
               
               
                 catcaccccc atggtcagag ttcaacaatg tggaattcac gttaaacaag ct  
               
               
                 #tgctaccc    600  
               
               
                   
               
               
                 actggccttt gggattcctg ctatcctcat ggctgtatcc ctgatcgtgt tc  
               
               
                 #atcatcgg    660  
               
               
                   
               
               
                 cagtgggatg tacaagaagt tcaagccgca ggggaacatc ctgagcaaag tg  
               
               
                 #gtgaagtg    720  
               
               
                   
               
               
                 catctgcttt gccatcaaaa ataggtttag gcaccgcagt aagcagtttc cc  
               
               
                 #aagagggc    780  
               
               
                   
               
               
                 gcactggctg gactgggcta aggagaaata cgacgagcgg cttatcgcgc ag  
               
               
                 #atcaagat    840  
               
               
                   
               
               
                 ggttacgagg gtgctgttcc tgtacatccc actccccatg ttctgggcct tg  
               
               
                 #tttgatca    900  
               
               
                   
               
               
                 gcagggttcc agatggacgc tgcaagcgac gaccatgtcc gggagaattg ga  
               
               
                 #atccttga    960  
               
               
                   
               
               
                 aattcagccg gatcagatgc agactgtgaa caccatcttg attattatcc tg  
               
               
                 #gtccccat   1020  
               
               
                   
               
               
                 catggacgcc gtggtgtatc ctctgattgc aaagtgtggc ctcaacttca cc  
               
               
                 #tctctgaa   1080  
               
               
                   
               
               
                 gaagatgacg attgggatgt tcctggcttc catggccttc gtggcagctg ca  
               
               
                 #atcctgca   1140  
               
               
                   
               
               
                 ggtggaaatc gataaaactc ttcctgtctt ccccaaagcc aatgaagtcc aa  
               
               
                 #attaaagt   1200  
               
               
                   
               
               
                 tctgaatgta ggaagtgaga acatgatcat ctctcttcct gggcagacgg tg  
               
               
                 #acgctcaa   1260  
               
               
                   
               
               
                 ccagatgtct caaacgaatg aattcatgac tttcaatgaa gacacactga ca  
               
               
                 #agcataaa   1320  
               
               
                   
               
               
                 catcacttcc ggatcacaag tcaccatgat cacacccagc cttgaggcag gc  
               
               
                 #cagcgcca   1380  
               
               
                   
               
               
                 caccctgctg gtgtgggccc ccaataacta ccgagtggtc aatgacggcc tg  
               
               
                 #acccagaa   1440  
               
               
                   
               
               
                 gtcagacaaa ggagaaaatg gaatcaggtt tgtgaacact tacagccagc cc  
               
               
                 #atcaacgt   1500  
               
               
                   
               
               
                 cacgatgagc gggaaagttt acgaacacat cgccagctac aatgccagcg ag  
               
               
                 #tatcagtt   1560  
               
               
                   
               
               
                 tttcacttct ggagtaaagg gcttcaccgt cagctcggca ggcatctcgg ag  
               
               
                 #cagtgcag   1620  
               
               
                   
               
               
                 gcgggacttt gagtctccgt acctggagtt tggcagcgcg tacacgtacc tg  
               
               
                 #atcacgag   1680  
               
               
                   
               
               
                 ccaggctact ggctgccccc aagtgacgga gtttgaagat attccgccca ac  
               
               
                 #acaatgaa   1740  
               
               
                   
               
               
                 catggcttgg caaatcccac agtacttcct catcacctct ggcgaggtgg tc  
               
               
                 #ttctccat   1800  
               
               
                   
               
               
                 cacgggcctg gagttctcct attctcaggc tccttccaac atgaagtcgg tg  
               
               
                 #ctgcagga   1860  
               
               
                   
               
               
                 ccggtggctg ctgacggtgg ctgtgggcaa catcattgtg ctcatcgtgg cc  
               
               
                 #ggcgcggg   1920  
               
               
                   
               
               
                 ccagatcaac aagcagtggg ccgagtacat cctctttgcc gccctgctcc tg  
               
               
                 #gtcgtctg   1980  
               
               
                   
               
               
                 tgtcatattt gccatcatgg ctcgattcta tacgtatgtc aacccggccg ag  
               
               
                 #atcgaggc   2040  
               
               
                   
               
               
                 tcagtttgaa gaagatgaga agaaaaagaa cccagaaaag aacgacctct ac  
               
               
                 #ccctccgt   2100  
               
               
                   
               
               
                 ggcgcccgtc tcacagacac agatgtgagt ctggaggcgg tgtaggaggc cc  
               
               
                 #acgcctgg   2160  
               
               
                   
               
               
                 cgtgcactgt gacctctgtc cgagggcgca ggacgtaccc ctgggcagcc cc  
               
               
                 #ggaagggg   2220  
               
               
                   
               
               
                 aggacttgag aactgtgaac cagaccacga aagctatgtt ctgagcagcc ag  
               
               
                 #tgatgagt   2280  
               
               
                   
               
               
                 ccaaaactct gaaagaaatc ttgttgaaag tcttatttaa aacacacaca ca  
               
               
                 #cacacaca   2340  
               
               
                   
               
               
                 cacacacaca cacacttttc caacactgac agcctaccca tgttaactcc tt  
               
               
                 #ctctacca   2400  
               
               
                   
               
               
                 atgcaaatgc tgttattttg gactaactta attttgaaca ctgttctatg tt  
               
               
                 #gcttgtat   2460  
               
               
                   
               
               
                 tctaacatcc ttaggaaagg caatgttaag agaggcagga ggcaatgcca aa  
               
               
                 #gttgaata   2520  
               
               
                   
               
               
                 tgtaggtgtc agaatggtat ataccacata ttacttagta ttaactgaaa ac  
               
               
                 #ctcaactt   2580  
               
               
                   
               
               
                 tgaggttttg ttctattttt tccactcctt acctcttttt aacctgtgga ca  
               
               
                 #actcaaaa   2640  
               
               
                   
               
               
                 ggaccactca gataaaggcc agtaaagatt ttttttgccg ttttgatgaa at  
               
               
                 #aaaataat   2700  
               
               
                   
               
               
                 gttcctaag                 
               
               
                 #                   
               
               
                 #                   
               
               
                 #       2709  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 6  
               
               
                 &lt;211&gt; LENGTH: 2914  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Gallus gallus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 6  
               
               
                   
               
               
                 gctctctgtc cgtccctcgg tccctccgtc cctccgtccc cgcgcggccg cc  
               
               
                 #agcagcgt     60  
               
               
                   
               
               
                 gccggcccca tggctgcaaa aagtaagagt aagggccgat cagtgccgaa ct  
               
               
                 #gctttggc    120  
               
               
                   
               
               
                 taccccttga gcatcttctt catcgtcatc aatgagttct gcgagaggtt ct  
               
               
                 #cctactat    180  
               
               
                   
               
               
                 ggcatgcgag ctgtgctcgt attgtatttc aagtacttcc tgcggtggga tg  
               
               
                 #acaacttt    240  
               
               
                   
               
               
                 tctacagcca tctaccacac gtttgttgct ctgtgctact tgacgcccat cc  
               
               
                 #tgggagcg    300  
               
               
                   
               
               
                 ctcattgcag actcatggct gggaaagttt aagaccattg tctccctgtc ca  
               
               
                 #ttgtctat    360  
               
               
                   
               
               
                 acaattgggc aggcagtcat ggctgtaagc tccataaacg acatgacaga tc  
               
               
                 #aaaacaga    420  
               
               
                   
               
               
                 gatggcaatc ctgataatat tgcggtgcac attgccctgt ctatgactgg ct  
               
               
                 #tgattctc    480  
               
               
                   
               
               
                 atcgcgcttg gaactggtgg gatcaaacct tgtgtctcag catttggtgg gg  
               
               
                 #atcagttt    540  
               
               
                   
               
               
                 gaagaacatc aggaaaaaca aagaagtaga ttcttctcta tcttttattt gt  
               
               
                 #ccattaat    600  
               
               
                   
               
               
                 gctggaagtc tcatatccac tataatcacc ccaattctca gagctcaaga at  
               
               
                 #gtggcatt    660  
               
               
                   
               
               
                 cacagcagac agcagtgcta cccgctggca tttggagttc ccgctgccct ca  
               
               
                 #tggctgtt    720  
               
               
                   
               
               
                 tcattagttg tgttcatagc tggaagtgga atgtacaaaa aagttcaacc gc  
               
               
                 #aaggcaat    780  
               
               
                   
               
               
                 ataatggttc gagtttgtaa atgcattgga tttgccatta aaaacaggtt tc  
               
               
                 #ggcatcgc    840  
               
               
                   
               
               
                 agcaaagagt atcccaaaag agagcactgg ctagactggg caagcgagaa gt  
               
               
                 #atgataaa    900  
               
               
                   
               
               
                 cgactgattg ctcagaccaa gatggtgttg aaggtgcttt tcctttacat cc  
               
               
                 #ctctcccg    960  
               
               
                   
               
               
                 atgttctggg cactttttga ccagcaggga tcgagatgga cactgcaagc ca  
               
               
                 #caactatg   1020  
               
               
                   
               
               
                 gatggggact ttggagctat gcagattcag ccagaccaaa tgcagactgt ca  
               
               
                 #atccaatc   1080  
               
               
                   
               
               
                 ctgattataa taatggtccc agttgtagat gctgtgattt atcctttaat cc  
               
               
                 #agaaatgc   1140  
               
               
                   
               
               
                 aagatcaatt ttacgcccct gaggaggatc actgttggca tgttccttgc tg  
               
               
                 #gtctggct   1200  
               
               
                   
               
               
                 ttcgttgctg ctgctctttt gcaagtgcaa atagataaaa ctcttccagt tt  
               
               
                 #tccctgca   1260  
               
               
                   
               
               
                 gctggacagg cccaaatcaa aataataaat ctaggtgata gcaatgcgaa tg  
               
               
                 #ttacattt   1320  
               
               
                   
               
               
                 ctgcctaatc ttcagaacgt gactgtcctt cccatggagt caacaggcta ca  
               
               
                 #ggatgttt   1380  
               
               
                   
               
               
                 gagtcttccc agctaaaatc tgtaatggta aattttggga gtgagagtag aa  
               
               
                 #gtgaaaat   1440  
               
               
                   
               
               
                 atcgactcaa taagcagcaa tacgcatact gtcaccatca agaatgcagc ag  
               
               
                 #ccggcatt   1500  
               
               
                   
               
               
                 gtttctagct tgcggtctga taatttcaca tcaaaaccag aagaaggaaa ga  
               
               
                 #atctagtc   1560  
               
               
                   
               
               
                 aggtttgtaa ataatttgcc tcagacagtc aacatcacta tgggtgacac ga  
               
               
                 #cttttgga   1620  
               
               
                   
               
               
                 atactggaag agacaagtat cagtaattac agtccgttct caggaggaag aa  
               
               
                 #catatgat   1680  
               
               
                   
               
               
                 atagtgataa ctgcaggttc aactaattgc aaaccaactt cagagaaatt ag  
               
               
                 #gatatggt   1740  
               
               
                   
               
               
                 ggtgcttata cgatcgtaat taatgagtgt tctggagatg tgactcaatt aa  
               
               
                 #gatacatt   1800  
               
               
                   
               
               
                 gaagatatcc aacccaatac agtccatatg gcttggcaga tccctcagta tt  
               
               
                 #tcatactt   1860  
               
               
                   
               
               
                 acatgtggag aagtagtctt ctctgtcact gggctggagt tttcatactc ac  
               
               
                 #aggcacca   1920  
               
               
                   
               
               
                 tctaatatga agtcagtgct gcaagcagga tggctgctaa cagtggctgt cg  
               
               
                 #gtaacata   1980  
               
               
                   
               
               
                 attgtcctta tcgtggctgg agcatccaaa ctcagtgagc agtgggcaga at  
               
               
                 #atgttctc   2040  
               
               
                   
               
               
                 tttgctgcct tgctttttgc agtttgcatt atttttgctg tcatggcata tt  
               
               
                 #tttataca   2100  
               
               
                   
               
               
                 tatactgatc caaatgaggt tgaagcccaa cttgatgaag aagaaaagaa ga  
               
               
                 #aacaaata   2160  
               
               
                   
               
               
                 aaacaggatc cagacttgca cggaaaagaa tctgaagctg tctctcagat gt  
               
               
                 #agaaggtg   2220  
               
               
                   
               
               
                 tattcaagag catttgtaaa tcatggtagc ctgttaactg tccctgcaat aa  
               
               
                 #caggaatc   2280  
               
               
                   
               
               
                 agggtattgc tgacatcact gggtaatata ccttgtggga gagactaaga aa  
               
               
                 #cactgttc   2340  
               
               
                   
               
               
                 tgacttaaca tacagcctct tgggaagcaa gacgaaatga ttaatctctt gt  
               
               
                 #acagaagc   2400  
               
               
                   
               
               
                 tggcatcctg aggaaactcc tgcagaattt gcactcttaa aatgtacctc aa  
               
               
                 #gctcaata   2460  
               
               
                   
               
               
                 ccatagcatt aaaatattga aattgcactt ggcactatta gacactctaa aa  
               
               
                 #agatgtat   2520  
               
               
                   
               
               
                 ttttatactg tatttcaatt ttataatgtg gaggggtggg gaaaaaggtg tt  
               
               
                 #gccaagaa   2580  
               
               
                   
               
               
                 atagtaattg aagccaaact gtctgcgtga cccttctagc ctcactgtta ct  
               
               
                 #tgaaagca   2640  
               
               
                   
               
               
                 ggtcacatgt gccttaaatt cttttctatg tccttaagaa taataggaga aa  
               
               
                 #ggttctta   2700  
               
               
                   
               
               
                 gatttctcag attaaaatgt ctctgctcca catagcagga acttggacat gc  
               
               
                 #actgtgat   2760  
               
               
                   
               
               
                 gtgctttatg tgcctattat taactgccca ttggtatgtt cttaattaat tg  
               
               
                 #tgttaatg   2820  
               
               
                   
               
               
                 ggatgtccac tgaggtgaac agacaatggc aaattatatt ttgaataacc ac  
               
               
                 #caagaata   2880  
               
               
                   
               
               
                 aaacttgtgt tgtaacaaaa aaaaaaaaaa aaaa        
               
               
                 #                   
               
               
                 #      2914  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 7  
               
               
                 &lt;211&gt; LENGTH: 1840  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Canis familiaris  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 7  
               
               
                   
               
               
                 catcttcttc atcgtggtca atgagttctg tgaaagattt tcctactatg ga  
               
               
                 #atgagagc     60  
               
               
                   
               
               
                 actcctgatt ctgtacttca gacggttcat cgggtgggac gataatctgt cc  
               
               
                 #acggccat    120  
               
               
                   
               
               
                 ctaccacacg tttgtggctc tgtgctacct gacgccgatc ctcggcgcac tg  
               
               
                 #atcgcaga    180  
               
               
                   
               
               
                 ctcctggctg ggaaagttca agacaatcgt gtcactctcc attgtctaca ca  
               
               
                 #attggaca    240  
               
               
                   
               
               
                 ggcggtcact gcagtaagct caattaatga cctcacagac tataacaaag at  
               
               
                 #ggaactcc    300  
               
               
                   
               
               
                 tgacaatctg tccgtgcatg tggcactgtc catgattggc ctggccctga ta  
               
               
                 #gctctggg    360  
               
               
                   
               
               
                 aactggagga ataaagccct gtgtgtctgc atttggtgga gaccagtttg aa  
               
               
                 #gagggcca    420  
               
               
                   
               
               
                 ggaaaaacaa agaaacagat tcttttccat cttttatttg gccattaatg ct  
               
               
                 #ggaagctt    480  
               
               
                   
               
               
                 gatttccact attgtcactc ccatgctcag agttcacgaa tgtggaattt ac  
               
               
                 #agtcagaa    540  
               
               
                   
               
               
                 agcttgttac ccactggcat ttggggttcc tgctgctctc atggccgtat ct  
               
               
                 #ctgattgt    600  
               
               
                   
               
               
                 atttgtcatt ggcagtggaa tgtacaagaa gtttcagccc cagggtaatg tc  
               
               
                 #atgggtaa    660  
               
               
                   
               
               
                 agttgtcaag tgcattggtt ttgccctcaa aaataggttt aggcaccgga gt  
               
               
                 #aagcagtt    720  
               
               
                   
               
               
                 tcccaagagg gagcactggc tggactgggc taaagagaaa tacgatgagc gg  
               
               
                 #ctcatctc    780  
               
               
                   
               
               
                 tcaaattaag atggtcacaa aagtgatgtt cttgtacatc ccactcccaa tg  
               
               
                 #ttctgggc    840  
               
               
                   
               
               
                 cctgtttgac cagcagggct ccaggtggac actgcaagca acagctatga gt  
               
               
                 #gggaaaat    900  
               
               
                   
               
               
                 tggacttctt gaagttcagc cagatcagat gcagactgtg aatgccatct tg  
               
               
                 #attgtcgt    960  
               
               
                   
               
               
                 catggtcccc atcatggatg ccgtggtgta ccctctgatt gcaaaatgtg gc  
               
               
                 #ttcaattt   1020  
               
               
                   
               
               
                 cacctccttg aagaggatga cagttggaat gttcctggct tccatggcct tc  
               
               
                 #gtgatggc   1080  
               
               
                   
               
               
                 ggcgattgtt cagctggaaa ttgataaaac tcttccagtc ttccccaaac aa  
               
               
                 #aatgaagt   1140  
               
               
                   
               
               
                 ccaaatcaaa gtactgaata taggaaatgg tgccatgaat gtatcttttc ct  
               
               
                 #ggagcggt   1200  
               
               
                   
               
               
                 ggtgacagtt agccaaatga gtcaatcaga tggatttatg acttttgatg ta  
               
               
                 #gacaaact   1260  
               
               
                   
               
               
                 gacaagtata aacatttctt ccactggatc accagtcatt ccagtgactt at  
               
               
                 #aactttga   1320  
               
               
                   
               
               
                 gcagggccat cgccataccc ttctagtatg ggcccccaat aattaccgag tg  
               
               
                 #gtaaagga   1380  
               
               
                   
               
               
                 tggccttaac cagaagccag aaaaaggaga aaatggaatc agatttataa at  
               
               
                 #agtcttaa   1440  
               
               
                   
               
               
                 tgagagcctc aacatcacca tgggcgacaa agtttatgtg aatgtcacca gt  
               
               
                 #cacaatgc   1500  
               
               
                   
               
               
                 cagcgagtat cagttctttt ctttgggcac aaaaaacatt acaataagtt ca  
               
               
                 #acacaaca   1560  
               
               
                   
               
               
                 gatctcacaa aattgtacaa aagttctcca atcatccaac cttgaatttg gt  
               
               
                 #agtgcata   1620  
               
               
                   
               
               
                 tacctatgta atcggaacgc agagcactgg ctgccctgaa ttgcatatgt tt  
               
               
                 #gaagatat   1680  
               
               
                   
               
               
                 ttcacccaac acagttaaca tggctctgca gatcccgcag tacttcctca tc  
               
               
                 #acctgcgg   1740  
               
               
                   
               
               
                 cgaggtggtt ttctctgtca caggactgga gttctcatat tctcaggccc cc  
               
               
                 #tccaacat   1800  
               
               
                   
               
               
                 gaagtcggtg cttcaggcgg gatggctgct gacagtggct      
               
               
                 #                   
               
               
                 #  1840  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 8  
               
               
                 &lt;211&gt; LENGTH: 1995  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Canis familiaris  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 8  
               
               
                   
               
               
                 catcttcttc atcgtggtca atgagttctg tgaaagattt tcctactatg ga  
               
               
                 #atgagagc     60  
               
               
                   
               
               
                 actcctgatt ctgtacttca gacggttcat cgggtgggac gataatctgt cc  
               
               
                 #acggccat    120  
               
               
                   
               
               
                 ctaccacacg tttgtggctc tgtgctacct gacgccgatc ctcggcgcac tg  
               
               
                 #atcgcaga    180  
               
               
                   
               
               
                 ctcctggctg ggaaagttca agacaatcgt gtcactctcc attgtctaca ca  
               
               
                 #attggaca    240  
               
               
                   
               
               
                 ggcggtcact gcagtaagct caattaatga cctcacagac tataacaaag at  
               
               
                 #ggaactcc    300  
               
               
                   
               
               
                 tgacaatctg tccgtgcatg tggcactgtc catgattggc ctggccctga ta  
               
               
                 #gctctggg    360  
               
               
                   
               
               
                 aactggagga ataaagccct gtgtgtctgc atttggtgga gaccagtttg aa  
               
               
                 #gagggcca    420  
               
               
                   
               
               
                 ggaaaaacaa agaaacagat tcttttccat cttttatttg gccattaatg ct  
               
               
                 #ggaagctt    480  
               
               
                   
               
               
                 gatttccact attgtcactc ccatgctcag agttcacgaa tgtggaattt ac  
               
               
                 #agtcagaa    540  
               
               
                   
               
               
                 agcttgttac ccactggcat ttggggttcc tgctgctctc atggccgtat ct  
               
               
                 #ctgattgt    600  
               
               
                   
               
               
                 atttgtcatt ggcagtggaa tgtacaagaa gtttcagccc cagggtaatg tc  
               
               
                 #atgggtaa    660  
               
               
                   
               
               
                 agttgtcaag tgcattggtt ttgccctcaa aaataggttt aggcaccgga gt  
               
               
                 #aagcagtt    720  
               
               
                   
               
               
                 tcccaagagg gagcactggc tggactgggc taaagagaaa tacgatgagc gg  
               
               
                 #ctcatctc    780  
               
               
                   
               
               
                 tcaaattaag atggtcacaa aagtgatgtt cttgtacatc ccactcccaa tg  
               
               
                 #ttctgggc    840  
               
               
                   
               
               
                 cctgtttgac cagcagggct ccaggtggac actgcaagca acagctatga gt  
               
               
                 #gggaaaat    900  
               
               
                   
               
               
                 tggacttctt gaagttcagc cagatcagat gcagactgtg aatgccatct tg  
               
               
                 #attgtcgt    960  
               
               
                   
               
               
                 catggtcccc atcatggatg ccgtggtgta ccctctgatt gcaaaatgtg gc  
               
               
                 #ttcaattt   1020  
               
               
                   
               
               
                 cacctccttg aagaggatga cagttggaat gttcctggct tccatggcct tc  
               
               
                 #gtgatggc   1080  
               
               
                   
               
               
                 ggcgattgtt cagctggaaa ttgataaaac tcttccagtc ttccccaaac aa  
               
               
                 #aatgaagt   1140  
               
               
                   
               
               
                 ccaaatcaaa gtactgaata taggaaatgg tgccatgaat gtatcttttc ct  
               
               
                 #ggagcggt   1200  
               
               
                   
               
               
                 ggtgacagtt agccaaatga gtcaatcaga tggatttatg acttttgatg ta  
               
               
                 #gacaaact   1260  
               
               
                   
               
               
                 gacaagtata aacatttctt ccactggatc accagtcatt ccagtgactt at  
               
               
                 #aactttga   1320  
               
               
                   
               
               
                 gcagggccat cgccataccc ttctagtatg ggcccccaat aattaccgag tg  
               
               
                 #gtaaagga   1380  
               
               
                   
               
               
                 tggccttaac cagaagccag aaaaaggaga aaatggaatc agatttataa at  
               
               
                 #agtcttaa   1440  
               
               
                   
               
               
                 tgagagcctc aacatcacca tgggcgacaa agtttatgtg aatgtcacca gt  
               
               
                 #cacaatgc   1500  
               
               
                   
               
               
                 cagcgagtat cagttctttt ctttgggcac aaaaaacatt acaataagtt ca  
               
               
                 #acacaaca   1560  
               
               
                   
               
               
                 gatctcacaa aattgtacaa aagttctcca atcatccaac cttgaatttg gt  
               
               
                 #agtgcata   1620  
               
               
                   
               
               
                 tacctatgta atcggaacgc agagcactgg ctgccctgaa ttgcatatgt tt  
               
               
                 #gaagatat   1680  
               
               
                   
               
               
                 ttcacccaac acagttaaca tggctctgca gatcccgcag tacttcctca tc  
               
               
                 #acctgcgg   1740  
               
               
                   
               
               
                 cgaggtggtt ttctctgtca caggactgga gttctcatat tctcaggccc cc  
               
               
                 #tccaacat   1800  
               
               
                   
               
               
                 gaagtcggtg cttcaggcgg gatggctgct gacagtggct tgttggcaac at  
               
               
                 #cattgtgc   1860  
               
               
                   
               
               
                 tcattgtggc aggagcaggc cagttcagtg aacagtgggc tgaatacatc ct  
               
               
                 #atttgcgg   1920  
               
               
                   
               
               
                 cattgcttct ggttgtctgt gtaatatttg ccatcatggc ccggttttac ac  
               
               
                 #ttacgtca   1980  
               
               
                   
               
               
                 atccagcaga gattg               
               
               
                 #                   
               
               
                 #                   
               
               
                 #  1995  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 9  
               
               
                 &lt;211&gt; LENGTH: 381  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Canis familiaris  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 9  
               
               
                   
               
               
                 tggctgggga agttcaagac aatcgtgtca ctctccattg tctacacaat tg  
               
               
                 #gacaggcg     60  
               
               
                   
               
               
                 gtcactgcag taagctcaat taatgacctc acagactata acaaagatgg aa  
               
               
                 #ctcctgac    120  
               
               
                   
               
               
                 aatctgtccg tgtatgtggc actgtccatg attggcctgg ccctgatagc tc  
               
               
                 #tgggaact    180  
               
               
                   
               
               
                 ggaggaataa agccctgtgt gtctgcattt ggtggagacc agtttgaaga gg  
               
               
                 #gccaggaa    240  
               
               
                   
               
               
                 aaacaaagaa acagattctt ttccatcttt tatttggcca ttaatgctgg aa  
               
               
                 #gcttgatt    300  
               
               
                   
               
               
                 tccactattg tcactcccat gctcagagtt cacgaatgtg gaatttacag tc  
               
               
                 #agaaagct    360  
               
               
                   
               
               
                 tgctacccac tggcctttgg g            
               
               
                 #                   
               
               
                 #                 381  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 10  
               
               
                 &lt;211&gt; LENGTH: 4  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Artificial Sequence  
               
               
                 &lt;220&gt; FEATURE:  
               
               
                 &lt;223&gt; OTHER INFORMATION: tetrapeptide  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 10  
               
               
                   
               
               
                 Met Gly Met Met  
               
               
                  1  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 11  
               
               
                 &lt;211&gt; LENGTH: 1410  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Canis familiaris  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 11  
               
               
                   
               
               
                 catcttcttc atcgtggtca atgagttctg tgaaagattt tcctactatg ga  
               
               
                 #atgagagc     60  
               
               
                   
               
               
                 actcctgatt ctgtacttca gacggttcat cgggtgggac gataatctgt cc  
               
               
                 #acggccat    120  
               
               
                   
               
               
                 ctaccacacg tttgtggctc tgtgctacct gacgccgatc ctcggcgcac tg  
               
               
                 #atcgcaga    180  
               
               
                   
               
               
                 ctcctggctg ggaaagttca agacaatcgt gtcactctcc attgtctaca ca  
               
               
                 #attggaca    240  
               
               
                   
               
               
                 ggcggtcact gcagtaagct caattaatga cctcacagac tataacaaag at  
               
               
                 #ggaactcc    300  
               
               
                   
               
               
                 tgacaatctg tccgtgcatg tggcactgtc catgattggc ctggccctga ta  
               
               
                 #gctctggg    360  
               
               
                   
               
               
                 aactggagga ataaagccct gtgtgtctgc atttggtgga gaccagtttg aa  
               
               
                 #gagggcca    420  
               
               
                   
               
               
                 ggaaaaacaa agaaacagat tcttttccat cttttatttg gccattaatg ct  
               
               
                 #ggaagctt    480  
               
               
                   
               
               
                 gatttccact attgtcactc ccatgctcag agttcacgaa tgtggaattt ac  
               
               
                 #agtcagaa    540  
               
               
                   
               
               
                 agcttgttac ccactggcat ttggggttcc tgctgctctc atggccgtat ct  
               
               
                 #ctgattgt    600  
               
               
                   
               
               
                 atttgtcatt ggcagtggaa tgtacaagaa gtttcagccc cagggtaatg tc  
               
               
                 #atgggtaa    660  
               
               
                   
               
               
                 agttgtcaag tgcattggtt ttgccctcaa aaataggttt aggcaccgga gt  
               
               
                 #aagcagtt    720  
               
               
                   
               
               
                 tcccaagagg gagcactggc tggactgggc taaagagaaa tacgatgagc gg  
               
               
                 #ctcatctc    780  
               
               
                   
               
               
                 tcaaattaag atggtcacaa aagtgatgtt cttgtacatc ccactcccaa tg  
               
               
                 #ttctgggc    840  
               
               
                   
               
               
                 cctgtttgac cagcagggct ccaggtggac actgcaagca acagctatga gt  
               
               
                 #gggaaaat    900  
               
               
                   
               
               
                 tggacttctt gaagttcagc cagatcagat gcagactgtg aatgccatct tg  
               
               
                 #attgtcgt    960  
               
               
                   
               
               
                 catggtcccc atcatggatg ccgtggtgta ccctctgatt gcaaaatgtg gc  
               
               
                 #ttcaattt   1020  
               
               
                   
               
               
                 cacctccttg aagaggatga cagttggaat gttcctggct tccatggcct tc  
               
               
                 #gtgatggc   1080  
               
               
                   
               
               
                 ggcgattgtt cagctggaaa ttgataaaac tcttccagtc ttccccaaac aa  
               
               
                 #aatgaagt   1140  
               
               
                   
               
               
                 ccaaatcaaa gtactgaata taggaaatgg tgccatgaat gtatcttttc ct  
               
               
                 #ggagcggt   1200  
               
               
                   
               
               
                 ggtgacagtt agccaaatga gtcaatcaga tggatttatg acttttgatg ta  
               
               
                 #gacaaact   1260  
               
               
                   
               
               
                 gacaagtata aacatttctt ccactggatc accagtcatt ccagtgactt at  
               
               
                 #aactttga   1320  
               
               
                   
               
               
                 gcagggccat cgccataccc ttctagtatg ggcccccaat aattaccgag tg  
               
               
                 #gtaaagga   1380  
               
               
                   
               
               
                 tggccttaac cagaagccag aaaaagggag          
               
               
                 #                   
               
               
                 #         1410  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 12  
               
               
                 &lt;211&gt; LENGTH: 670  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Canis familiaris  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 12  
               
               
                   
               
               
                 gccatcgcca tacccttcta gtatgggccc ccaataatta ccgagtggta aa  
               
               
                 #ggatggcc     60  
               
               
                   
               
               
                 ttaaccagaa gccagaaaaa ggagaaaatg gaatcagatt tataaatagt ct  
               
               
                 #taatgaga    120  
               
               
                   
               
               
                 gcctcaacat caccatgggc gacaaagttt atgtgaatgt caccagtcac aa  
               
               
                 #tgccagcg    180  
               
               
                   
               
               
                 agtatcagtt cttttctttg ggcacaaaaa acattacaat aagttcaaca ca  
               
               
                 #acagatct    240  
               
               
                   
               
               
                 cacaaaattg tacaaaagtt ctccaatcat ccaaccttga atttggtagt gc  
               
               
                 #atatacct    300  
               
               
                   
               
               
                 atgtaatcgg aacgcagagc actggctgcc ctgaattgca tatgtttgaa ga  
               
               
                 #tatttcac    360  
               
               
                   
               
               
                 ccaacacagt taacatggct ctgcagatcc cgcagtactt cctcatcacc tg  
               
               
                 #cggcgagg    420  
               
               
                   
               
               
                 tggttttctc tgtcacagga ctggagttct catattctca ggccccctcc aa  
               
               
                 #catgaagt    480  
               
               
                   
               
               
                 cggtgcttca ggcgggatgg ctgctgacag tggcttgttg gcaacatcat tg  
               
               
                 #tgctcatt    540  
               
               
                   
               
               
                 gtggcaggag caggccagtt cagtgaacag tgggctgaat acatcctatt tg  
               
               
                 #cggcattg    600  
               
               
                   
               
               
                 cttctggttg tctgtgtaat atttgccatc atggcccggt tttacactta cg  
               
               
                 #tcaatcca    660  
               
               
                   
               
               
                 gcagagattg                 
               
               
                 #                   
               
               
                 #                   
               
               
                 #       670  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 13  
               
               
                 &lt;211&gt; LENGTH: 662  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Canis familiaris  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 13  
               
               
                   
               
               
                 Ile Phe Phe Ile Val Val Asn Glu Phe Cys Gl  
               
               
                 #u Arg Phe Ser Tyr Tyr  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Gly Met Arg Ala Leu Leu Ile Leu Tyr Phe Ar  
               
               
                 #g Arg Phe Ile Gly Trp  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Asp Asp Asn Leu Ser Thr Ala Ile Tyr His Th  
               
               
                 #r Phe Val Ala Leu Cys  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Tyr Leu Thr Pro Ile Leu Gly Ala Leu Ile Al  
               
               
                 #a Asp Ser Trp Leu Gly  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Lys Phe Lys Thr Ile Val Ser Leu Ser Ile Va  
               
               
                 #l Tyr Thr Ile Gly Gln  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Ala Val Thr Ala Val Ser Ser Ile Asn Asp Le  
               
               
                 #u Thr Asp Tyr Asn Lys  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Asp Gly Thr Pro Asp Asn Leu Ser Val His Va  
               
               
                 #l Ala Leu Ser Met Ile  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Gly Leu Ala Leu Ile Ala Leu Gly Thr Gly Gl  
               
               
                 #y Ile Lys Pro Cys Val  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Ser Ala Phe Gly Gly Asp Gln Phe Glu Glu Gl  
               
               
                 #y Gln Glu Lys Gln Arg  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Asn Arg Phe Phe Ser Ile Phe Tyr Leu Ala Il  
               
               
                 #e Asn Ala Gly Ser Leu  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Ile Ser Thr Ile Val Thr Pro Met Leu Arg Va  
               
               
                 #l His Glu Cys Gly Ile  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Tyr Ser Gln Lys Ala Cys Tyr Pro Leu Ala Ph  
               
               
                 #e Gly Val Pro Ala Ala  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Leu Met Ala Val Ser Leu Ile Val Phe Val Il  
               
               
                 #e Gly Ser Gly Met Tyr  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Lys Lys Phe Gln Pro Gln Gly Asn Val Met Gl  
               
               
                 #y Lys Val Val Lys Cys  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Ile Gly Phe Ala Leu Lys Asn Arg Phe Arg Hi  
               
               
                 #s Arg Ser Lys Gln Phe  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Pro Lys Arg Glu His Trp Leu Asp Trp Ala Ly  
               
               
                 #s Glu Lys Tyr Asp Glu  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Arg Leu Ile Ser Gln Ile Lys Met Val Thr Ly  
               
               
                 #s Val Met Phe Leu Tyr  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Ile Pro Leu Pro Met Phe Trp Ala Leu Phe As  
               
               
                 #p Gln Gln Gly Ser Arg  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Trp Thr Leu Gln Ala Thr Ala Met Ser Gly Ly  
               
               
                 #s Ile Gly Leu Leu Glu  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Val Gln Pro Asp Gln Met Gln Thr Val Asn Al  
               
               
                 #a Ile Leu Ile Val Val  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Met Val Pro Ile Met Asp Ala Val Val Tyr Pr  
               
               
                 #o Leu Ile Ala Lys Cys  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Gly Phe Asn Phe Thr Ser Leu Lys Arg Met Th  
               
               
                 #r Val Gly Met Phe Leu  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Ala Ser Met Ala Phe Val Met Ala Ala Ile Va  
               
               
                 #l Gln Leu Glu Ile Asp  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Lys Thr Leu Pro Val Phe Pro Lys Gln Asn Gl  
               
               
                 #u Val Gln Ile Lys Val  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Leu Asn Ile Gly Asn Gly Ala Met Asn Val Se  
               
               
                 #r Phe Pro Gly Ala Val  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Val Thr Val Ser Gln Met Ser Gln Ser Asp Gl  
               
               
                 #y Phe Met Thr Phe Asp  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Val Asp Lys Leu Thr Ser Ile Asn Ile Ser Se  
               
               
                 #r Thr Gly Ser Pro Val  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Ile Pro Val Thr Tyr Asn Phe Glu Gln Gly Hi  
               
               
                 #s Arg His Thr Leu Leu  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Val Trp Ala Pro Asn Asn Tyr Arg Val Val Ly  
               
               
                 #s Asp Gly Leu Asn Gln  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Lys Pro Glu Lys Gly Glu Asn Gly Ile Arg Ph  
               
               
                 #e Ile Asn Ser Leu Asn  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Glu Ser Leu Asn Ile Thr Met Gly Asp Lys Va  
               
               
                 #l Tyr Val Asn Val Thr  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Ser His Asn Ala Ser Glu Tyr Gln Phe Phe Se  
               
               
                 #r Leu Gly Thr Lys Asn  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Ile Thr Ile Ser Ser Thr Gln Gln Ile Ser Gl  
               
               
                 #n Asn Cys Thr Lys Val  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Leu Gln Ser Ser Asn Leu Glu Phe Gly Ser Al  
               
               
                 #a Tyr Thr Tyr Val Ile  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Gly Thr Gln Ser Thr Gly Cys Pro Glu Leu Hi  
               
               
                 #s Met Phe Glu Asp Ile  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Ser Pro Asn Thr Val Asn Met Ala Leu Gln Il  
               
               
                 #e Pro Gln Tyr Phe Leu  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Ile Thr Cys Gly Glu Val Val Phe Ser Val Th  
               
               
                 #r Gly Leu Glu Phe Ser  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Tyr Ser Gln Ala Pro Ser Asn Met Lys Ser Va  
               
               
                 #l Leu Gln Ala Gly Trp  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Leu Leu Thr Val Ala Cys Trp Gln His His Cy  
               
               
                 #s Ala His Cys Gly Arg  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Ser Arg Pro Val Gln Thr Val Gly Ile His Pr  
               
               
                 #o Ile Cys Gly Ile Ala  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Ser Gly Cys Leu Cys Asn Ile Cys His His Gl  
               
               
                 #y Pro Val Leu His Leu  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Arg Gln Ser Ser Arg Asp  
               
               
                             660  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 14  
               
               
                 &lt;211&gt; LENGTH: 706  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Homo sapien  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 14  
               
               
                   
               
               
                 Met Ser Lys Ser His Ser Phe Phe Gly Tyr Pr  
               
               
                 #o Leu Ser Ile Phe Phe  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Ile Val Val Asn Glu Phe Cys Glu Arg Phe Se  
               
               
                 #r Tyr Tyr Gly Met Arg  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Ala Ile Leu Ile Leu Tyr Phe Thr Asn Phe Il  
               
               
                 #e Ser Trp Asp Asp Asn  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Leu Ser Thr Ala Ile Tyr His Thr Phe Val Al  
               
               
                 #a Leu Cys Tyr Leu Thr  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Pro Ile Leu Gly Ala Leu Ile Ala Asp Ser Tr  
               
               
                 #p Leu Gly Lys Phe Lys  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Thr Ile Val Ser Leu Ser Ile Val Tyr Thr Il  
               
               
                 #e Gly Gln Ala Val Thr  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Ser Val Ser Ser Ile Asn Asp Leu Thr Asp Hi  
               
               
                 #s Asn His Asp Gly Thr  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Pro Asp Ser Leu Pro Val His Val Val Leu Se  
               
               
                 #r Leu Ile Gly Leu Ala  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Leu Ile Ala Leu Gly Thr Gly Gly Ile Lys Pr  
               
               
                 #o Cys Val Ser Ala Phe  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Gly Gly Asp Gln Phe Glu Glu Gly Gln Glu Ly  
               
               
                 #s Gln Arg Asn Arg Phe  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Phe Ser Ile Phe Tyr Leu Ala Ile Asn Ala Gl  
               
               
                 #y Ser Leu Leu Ser Thr  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Ile Ile Thr Pro Met Leu Arg Val Gln Gln Cy  
               
               
                 #s Gly Ile His Ser Lys  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Gln Ala Cys Tyr Pro Leu Ala Phe Gly Val Pr  
               
               
                 #o Ala Ala Leu Met Ala  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Val Ala Leu Ile Val Phe Val Leu Gly Ser Gl  
               
               
                 #y Met Tyr Lys Lys Phe  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Lys Pro Gln Gly Asn Ile Met Gly Lys Val Al  
               
               
                 #a Lys Cys Ile Gly Phe  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Ala Ile Lys Asn Arg Phe Arg His Arg Ser Ly  
               
               
                 #s Ala Phe Pro Lys Arg  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Glu His Trp Leu Asp Trp Ala Lys Glu Lys Ty  
               
               
                 #r Asp Glu Arg Leu Ile  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Ser Gln Ile Lys Met Val Thr Arg Val Met Ph  
               
               
                 #e Leu Tyr Ile Pro Leu  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Pro Met Phe Trp Ala Leu Phe Asp Gln Gln Gl  
               
               
                 #y Ser Arg Trp Thr Leu  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Gln Ala Thr Thr Met Ser Gly Lys Ile Gly Al  
               
               
                 #a Leu Glu Ile Gln Pro  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Asp Gln Met Gln Thr Val Asn Ala Ile Leu Il  
               
               
                 #e Val Ile Met Val Pro  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Ile Phe Asp Ala Val Leu Tyr Pro Leu Ile Al  
               
               
                 #a Lys Cys Gly Phe Asn  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Phe Thr Ser Leu Lys Lys Met Ala Val Gly Me  
               
               
                 #t Val Leu Ala Ser Met  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Ala Phe Val Val Ala Ala Ile Val Gln Val Gl  
               
               
                 #u Ile Asp Lys Thr Leu  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Pro Val Phe Pro Lys Gly Asn Glu Val Gln Il  
               
               
                 #e Lys Val Leu Asn Ile  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Gly Asn Asn Thr Met Asn Ile Ser Leu Pro Gl  
               
               
                 #y Glu Met Val Thr Leu  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Gly Pro Met Ser Gln Thr Asn Ala Phe Met Th  
               
               
                 #r Phe Asp Val Asn Lys  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Leu Thr Arg Ile Asn Ile Ser Ser Pro Gly Se  
               
               
                 #r Pro Val Thr Ala Val  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Thr Asp Asp Phe Lys Gln Gly Gln Arg His Th  
               
               
                 #r Leu Leu Val Trp Ala  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Pro Asn His Tyr Gln Val Val Lys Asp Gly Le  
               
               
                 #u Asn Gln Lys Pro Glu  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Lys Gly Glu Asn Gly Ile Arg Phe Val Asn Th  
               
               
                 #r Phe Asn Glu Leu Ile  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Thr Ile Thr Met Ser Gly Lys Val Tyr Ala As  
               
               
                 #n Ile Ser Ser Tyr Asn  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Ala Ser Thr Tyr Gln Phe Phe Pro Ser Gly Il  
               
               
                 #e Lys Gly Phe Thr Ile  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Ser Ser Thr Glu Ile Pro Pro Gln Cys Gln Pr  
               
               
                 #o Asn Phe Asn Thr Phe  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Tyr Leu Glu Phe Gly Ser Ala Tyr Thr Tyr Il  
               
               
                 #e Val Gln Arg Lys Asn  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Asp Ser Cys Pro Glu Val Lys Val Phe Glu As  
               
               
                 #p Ile Ser Ala Asn Thr  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Val Asn Met Ala Leu Gln Ile Pro Gln Tyr Ph  
               
               
                 #e Leu Leu Thr Cys Gly  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Glu Val Val Phe Ser Val Thr Gly Leu Glu Ph  
               
               
                 #e Ser Tyr Ser Gln Ala  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Pro Ser Asn Met Lys Ser Val Leu Gln Ala Gl  
               
               
                 #y Trp Leu Leu Thr Val  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Ala Val Gly Asn Ile Ile Val Leu Ile Val Al  
               
               
                 #a Gly Ala Gly Gln Phe  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Ser Lys Gln Trp Ala Glu Tyr Ile Leu Phe Al  
               
               
                 #a Ala Leu Leu Leu Val  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Val Cys Val Ile Phe Ala Ile Met Ala Arg Ph  
               
               
                 #e Tyr Thr Tyr Ile Asn  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Pro Ala Glu Ile Glu Ala Gln Phe Asp Glu As  
               
               
                 #p Glu Lys Lys Asn Arg  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Leu Glu Lys Ser Asn Pro Tyr Phe Met Ser Gl  
               
               
                 #y Ala Asn Ser Gln Lys  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Gln Met  
               
               
                 705  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 15  
               
               
                 &lt;211&gt; LENGTH: 710  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Rattus norvegicus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 15  
               
               
                   
               
               
                 Met Gly Met Ser Lys Ser Arg Gly Cys Phe Gl  
               
               
                 #y Tyr Pro Leu Ser Ile  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Phe Phe Ile Val Val Asn Glu Phe Cys Glu Ar  
               
               
                 #g Phe Ser Tyr Tyr Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Met Arg Ala Leu Leu Val Leu Tyr Phe Arg As  
               
               
                 #n Phe Leu Gly Trp Asp  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Asp Asp Leu Ser Thr Ala Ile Tyr His Thr Ph  
               
               
                 #e Val Ala Leu Cys Tyr  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Leu Thr Pro Ile Leu Gly Ala Leu Ile Ala As  
               
               
                 #p Ser Trp Leu Gly Lys  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Phe Lys Thr Ile Val Ser Leu Ser Ile Val Ty  
               
               
                 #r Thr Ile Gly Gln Ala  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Val Ile Ser Val Ser Ser Ile Asn Asp Leu Th  
               
               
                 #r Asp His Asp His Asp  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Gly Ser Pro Asn Asn Leu Pro Leu His Val Al  
               
               
                 #a Leu Ser Met Ile Gly  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Leu Ala Leu Ile Ala Leu Gly Thr Gly Gly Il  
               
               
                 #e Lys Pro Cys Val Ser  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ala Phe Gly Gly Asp Gln Phe Glu Glu Gly Gl  
               
               
                 #n Glu Lys Gln Arg Asn  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Arg Phe Phe Ser Ile Phe Tyr Leu Ala Ile As  
               
               
                 #n Ala Gly Ser Leu Leu  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Ser Thr Ile Ile Thr Pro Ile Leu Arg Val Gl  
               
               
                 #n Gln Cys Gly Ile His  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Ser Gln Gln Ala Cys Tyr Pro Leu Ala Phe Gl  
               
               
                 #y Val Pro Ala Ala Leu  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Met Ala Val Ala Leu Ile Val Phe Val Leu Gl  
               
               
                 #y Ser Gly Met Tyr Lys  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Lys Phe Gln Pro Gln Gly Asn Ile Met Gly Ly  
               
               
                 #s Val Ala Lys Cys Ile  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Gly Phe Ala Ile Lys Asn Arg Phe Arg His Ar  
               
               
                 #g Ser Lys Ala Phe Pro  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Lys Arg Glu His Trp Leu Asp Trp Ala Lys Gl  
               
               
                 #u Lys Tyr Asp Glu Arg  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Leu Ile Ser Gln Ile Lys Met Val Thr Lys Va  
               
               
                 #l Met Phe Leu Tyr Ile  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Pro Leu Pro Met Phe Trp Ala Leu Phe Asp Gl  
               
               
                 #n Gln Gly Ser Arg Trp  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Thr Leu Gln Ala Thr Thr Met Thr Gly Lys Il  
               
               
                 #e Gly Thr Ile Glu Ile  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Gln Pro Asp Gln Met Gln Thr Val Asn Ala Il  
               
               
                 #e Leu Ile Val Ile Met  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Val Pro Ile Val Asp Ala Val Val Tyr Pro Le  
               
               
                 #u Ile Ala Lys Cys Gly  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Phe Asn Phe Thr Ser Leu Lys Lys Met Thr Va  
               
               
                 #l Gly Met Phe Leu Ala  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Ser Met Ala Phe Val Val Ala Ala Ile Val Gl  
               
               
                 #n Val Glu Ile Asp Lys  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Thr Leu Pro Val Phe Pro Ser Gly Asn Gln Va  
               
               
                 #l Gln Ile Lys Val Leu  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Asn Ile Gly Asn Asn Asp Met Ala Val Tyr Ph  
               
               
                 #e Pro Gly Lys Asn Val  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Thr Val Ala Gln Met Ser Gln Thr Asp Thr Ph  
               
               
                 #e Met Thr Phe Asp Val  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Asp Gln Leu Thr Ser Ile Asn Val Ser Ser Pr  
               
               
                 #o Gly Ser Pro Gly Val  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Thr Thr Val Ala His Glu Phe Glu Pro Gly Hi  
               
               
                 #s Arg His Thr Leu Leu  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Val Trp Gly Pro Asn Leu Tyr Arg Val Val Ly  
               
               
                 #s Asp Gly Leu Asn Gln  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Lys Pro Glu Lys Gly Glu Asn Gly Ile Arg Ph  
               
               
                 #e Val Ser Thr Leu Asn  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Glu Met Ile Thr Ile Lys Met Ser Gly Lys Va  
               
               
                 #l Tyr Glu Asn Val Thr  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Ser His Ser Ala Ser Asn Tyr Gln Phe Phe Pr  
               
               
                 #o Ser Gly Gln Lys Asp  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Tyr Thr Ile Asn Thr Thr Glu Ile Ala Pro As  
               
               
                 #n Cys Ser Ser Asp Phe  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Lys Ser Ser Asn Leu Asp Phe Gly Ser Ala Ty  
               
               
                 #r Thr Tyr Val Ile Arg  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Ser Arg Ala Ser Asp Gly Cys Leu Glu Val Ly  
               
               
                 #s Glu Phe Glu Asp Ile  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Pro Pro Asn Thr Val Asn Met Ala Leu Gln Il  
               
               
                 #e Pro Gln Tyr Phe Leu  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Leu Thr Cys Gly Glu Val Val Phe Ser Val Th  
               
               
                 #r Gly Leu Glu Phe Ser  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Tyr Ser Gln Ala Pro Ser Asn Met Lys Ser Va  
               
               
                 #l Leu Gln Ala Gly Trp  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Leu Leu Thr Val Ala Ile Gly Asn Ile Ile Va  
               
               
                 #l Leu Ile Val Ala Glu  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Ala Gly His Phe Asp Lys Gln Trp Ala Glu Ty  
               
               
                 #r Val Leu Phe Ala Ser  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Leu Leu Leu Val Val Cys Ile Ile Phe Ala Il  
               
               
                 #e Met Ala Arg Phe Tyr  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Thr Tyr Ile Asn Pro Ala Glu Ile Glu Ala Gl  
               
               
                 #n Phe Asp Glu Asp Glu  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Lys Lys Lys Gly Val Gly Lys Glu Asn Pro Ty  
               
               
                 #r Ser Ser Leu Glu Pro  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Val Ser Gln Thr Asn Met  
               
               
                 705                 7  
               
               
                 #10  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 16  
               
               
                 &lt;211&gt; LENGTH: 709  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Mus musculus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 16  
               
               
                   
               
               
                 Met Gly Met Ser Lys Ser Arg Gly Cys Phe Gl  
               
               
                 #y Tyr Pro Leu Ser Ile  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Phe Phe Ile Val Val Asn Glu Phe Cys Glu Ar  
               
               
                 #g Phe Ser Tyr Tyr Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Met Arg Ala Leu Leu Val Leu Tyr Phe Arg As  
               
               
                 #n Phe Leu Gly Trp Asp  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Asp Asn Leu Ser Thr Ala Ile Tyr His Thr Ph  
               
               
                 #e Val Ala Leu Cys Tyr  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Leu Thr Pro Ile Leu Gly Ala Leu Ile Ala As  
               
               
                 #p Ser Trp Leu Gly Lys  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Phe Lys Thr Ile Val Ser Leu Ser Ile Val Ty  
               
               
                 #r Thr Ile Gly Gln Ala  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Val Ile Ser Val Ser Ser Ile Asn Asp Leu Th  
               
               
                 #r Asp His Asp His Asn  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Gly Ser Pro Asp Ser Leu Pro Val His Val Al  
               
               
                 #a Leu Ser Met Val Gly  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Leu Ala Leu Ile Ala Leu Gly Thr Gly Gly Il  
               
               
                 #e Lys Pro Cys Val Ser  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ala Phe Gly Gly Asp Gln Phe Glu Glu Gly Gl  
               
               
                 #n Glu Lys Gln Arg Asn  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Arg Phe Phe Ser Ile Phe Tyr Leu Ala Ile As  
               
               
                 #n Gly Gly Ser Leu Leu  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Ser Thr Ile Ile Thr Pro Ile Leu Arg Val Gl  
               
               
                 #n Gln Cys Gly Ile His  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Ser Gln Gln Ala Cys Tyr Pro Leu Ala Phe Gl  
               
               
                 #y Val Pro Ala Ala Leu  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Met Ala Val Ala Leu Ile Val Phe Val Leu Gl  
               
               
                 #y Ser Gly Met Tyr Lys  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Lys Phe Gln Pro Gln Gly Asn Ile Met Gly Ly  
               
               
                 #s Val Ala Lys Cys Ile  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Gly Phe Ala Ile Lys Asn Arg Phe Arg His Ar  
               
               
                 #g Ser Lys Ala Tyr Pro  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Lys Arg Glu His Trp Leu Asp Trp Ala Lys Gl  
               
               
                 #u Lys Tyr Asp Glu Arg  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Leu Ile Ser Gln Ile Lys Met Val Thr Lys Va  
               
               
                 #l Met Phe Leu Phe Ile  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Pro Leu Pro Met Phe Trp Gly Leu Phe Asp Gl  
               
               
                 #n Gln Gly Ser Arg Trp  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Thr Leu Gln Ala Thr Thr Met Asn Gly Lys Il  
               
               
                 #e Gly Ala Asn Glu Ile  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Gln Pro Asp Gln Met Gln Thr Val Asn Ala Il  
               
               
                 #e Leu Asn Val Asn Asn  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Gly Pro Asn Val Asp Ala Val Val Tyr Arg Se  
               
               
                 #r Ile Ala Lys Cys Gly  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Phe Asn Phe Thr Ser Leu Lys Lys Met Thr Va  
               
               
                 #l Gly Met Phe Leu Ala  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Ser Met Ala Phe Val Val Ala Ala Ile Val Gl  
               
               
                 #n Val Glu Ile Asp Lys  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Thr Leu Pro Val Phe Pro Gly Gly Asn Gln Va  
               
               
                 #l Gln Ile Lys Val Leu  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Asn Ile Gly Asn Asn Asn Met Thr Val His Ph  
               
               
                 #e Pro Gly Asn Ser Val  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Thr Leu Ala Gln Met Ser Gln Thr Asp Thr Ph  
               
               
                 #e Met Thr Phe Asp Ile  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Asp Lys Leu Thr Ser Ile Asn Ile Ser Ser Se  
               
               
                 #r Gly Ser Pro Gly Val  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Thr Thr Val Ala His Asp Phe Glu Gln Gly Hi  
               
               
                 #s Arg His Asn Leu Leu  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Val Trp Glu Pro Ser Gln Tyr Arg Val Val Ly  
               
               
                 #s Asp Gly Pro Asn Gln  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Lys Pro Glu Lys Gly Glu Asn Gly Ile Arg Ph  
               
               
                 #e Val Asn Thr Leu Asn  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Glu Met Val Thr Asn Lys Met Ser Gly Lys Va  
               
               
                 #l Tyr Glu Lys Phe Thr  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Ser His Asn Ala Ser Gly Tyr Lys Phe Leu Pr  
               
               
                 #o Ser Gly Glu Lys Gln  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Tyr Thr Ile Asn Thr Thr Ala Val Ala Pro Th  
               
               
                 #r Cys Leu Thr Asp Phe  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Lys Ser Ser Asn Leu Asp Phe Gly Ser Ala Ty  
               
               
                 #r Thr Tyr Val Ile Arg  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Arg Ala Ser Asp Gly Cys Leu Glu Val Lys Gl  
               
               
                 #u Phe Glu Asp Ile Pro  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Pro Asn Thr Val Asn Met Ala Leu Gln Ile Pr  
               
               
                 #o Gln Tyr Phe Leu Leu  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Thr Cys Gly Glu Val Val Phe Ser Val Thr Gl  
               
               
                 #y Leu Glu Phe Ser Tyr  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Ser Gln Ala Pro Ser Asn Met Lys Ser Val Le  
               
               
                 #u Gln Ala Gly Trp Leu  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Leu Thr Val Ala Val Gly Asn Ile Ile Val Le  
               
               
                 #u Ile Val Ala Gly Ala  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Gly His Phe Pro Lys Gln Trp Ala Glu Tyr Il  
               
               
                 #e Leu Phe Ala Ser Leu  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Leu Leu Val Val Cys Val Ile Phe Ala Ile Me  
               
               
                 #t Ala Arg Phe Tyr Thr  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Tyr Ile Asn Pro Ala Glu Ile Glu Ala Gln Ph  
               
               
                 #e Asp Glu Asp Glu Lys  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Lys Lys Gly Ile Gly Lys Glu Asn Pro Tyr Se  
               
               
                 #r Ser Leu Glu Pro Val  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Ser Gln Thr Asn Met  
               
               
                 705  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 17  
               
               
                 &lt;211&gt; LENGTH: 707  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Ovis aries  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 17  
               
               
                   
               
               
                 Met Gly Met Ser Val Pro Lys Ser Cys Phe Gl  
               
               
                 #y Tyr Pro Leu Ser Ile  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Phe Phe Ile Val Val Asn Glu Phe Cys Glu Ar  
               
               
                 #g Phe Ser Tyr Tyr Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Met Arg Ala Leu Leu Ile Leu Tyr Phe Gln Ar  
               
               
                 #g Phe Leu Gly Trp Asn  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Asp Asn Leu Gly Thr Ala Ile Tyr His Thr Ph  
               
               
                 #e Val Ala Leu Cys Tyr  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Leu Thr Pro Ile Leu Gly Ala Leu Ile Ala As  
               
               
                 #p Ser Trp Leu Gly Lys  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Phe Lys Thr Ile Val Ser Leu Ser Ile Val Ty  
               
               
                 #r Thr Ile Gly Gln Val  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Val Ile Ala Val Ser Ser Ile Asn Asp Leu Th  
               
               
                 #r Asp Phe Asn His Asp  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Gly Thr Pro Asn Asn Ile Ser Val His Val Al  
               
               
                 #a Leu Ser Met Ile Gly  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Leu Val Leu Ile Ala Leu Gly Thr Gly Gly Il  
               
               
                 #e Lys Pro Cys Val Ser  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ala Phe Gly Gly Asp Gln Phe Glu Glu Gly Gl  
               
               
                 #n Glu Lys Gln Arg Asn  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Arg Phe Phe Ser Ile Phe Tyr Leu Ala Ile As  
               
               
                 #n Ala Gly Ser Leu Leu  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Ser Thr Ile Ile Thr Pro Met Leu Arg Val Gl  
               
               
                 #n Val Cys Gly Ile His  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Ser Lys Gln Ala Cys Tyr Pro Leu Ala Phe Gl  
               
               
                 #y Val Pro Ala Ala Leu  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Met Ala Val Ser Leu Ile Val Phe Val Ile Gl  
               
               
                 #y Ser Gly Met Tyr Lys  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Lys Val Gln Pro Gln Gly Asn Ile Met Ser Ly  
               
               
                 #s Val Ala Arg Cys Ile  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Gly Phe Ala Ile Lys Asn Arg Ile Ser His Ar  
               
               
                 #g Ser Lys Lys Phe Pro  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Lys Arg Glu His Trp Leu Asp Trp Ala Ser Gl  
               
               
                 #u Lys Tyr Asp Glu Arg  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Leu Ile Ser Gln Ile Lys Met Val Thr Arg Va  
               
               
                 #l Met Phe Leu Tyr Ile  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Pro Leu Pro Met Phe Trp Ala Leu Phe Asp Gl  
               
               
                 #n Gln Gly Ser Arg Trp  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Thr Leu Gln Ala Thr Thr Met Ser Gly Lys Il  
               
               
                 #e Gly Ile Ile Glu Ile  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Gln Pro Asp Gln Met Gln Thr Val Asn Ala Il  
               
               
                 #e Leu Ile Val Val Met  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Val Pro Ile Val Asp Ala Val Val Tyr Pro Le  
               
               
                 #u Ile Ala Lys Cys Gly  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Leu Asn Phe Thr Ser Leu Lys Lys Met Thr Va  
               
               
                 #l Gly Met Phe Leu Ala  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Ser Met Ala Phe Val Ala Ala Ala Ile Val Gl  
               
               
                 #n Val Asp Ile Asp Lys  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Thr Leu Pro Val Phe Pro Lys Gly Asn Glu Va  
               
               
                 #l Gln Ile Lys Val Leu  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Asn Ile Gly Asn Asn Ser Met Thr Val Ser Ph  
               
               
                 #e Pro Gly Thr Thr Val  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Thr Cys Asp Gln Met Ser Gln Thr Asn Gly Ph  
               
               
                 #e Leu Thr Phe Asn Val  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Asp Asn Leu Ser Ile Asn Ile Ser Ser Thr Gl  
               
               
                 #y Thr Pro Val Thr Pro  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Val Thr His Asn Phe Glu Ser Gly His Arg Hi  
               
               
                 #s Thr Leu Leu Val Trp  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Ala Pro Ser Asn Tyr Gln Val Val Lys Asp Gl  
               
               
                 #y Leu Asn Gln Lys Pro  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Glu Lys Gly Arg Asn Gly Ile Arg Phe Val As  
               
               
                 #n Ala Phe Gly Glu Ser  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Phe Gly Val Thr Met Asp Gly Glu Val Tyr As  
               
               
                 #n Asn Val Ser Gly His  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Asn Ala Ser Glu Tyr Leu Phe Phe Ser Ser Gl  
               
               
                 #y Val Lys Ser Phe Thr  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Ile Asn Ser Pro Glu Ile Ser Gln Gln Cys Gl  
               
               
                 #u Lys Gln Phe Lys Thr  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Ser Tyr Leu Glu Phe Gly Ser Ala Phe Thr Ty  
               
               
                 #r Val Ile Ser Arg Lys  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Ser Asp Gly Cys Pro Glu Pro Lys Ile Phe Gl  
               
               
                 #u Asp Ile Ser Pro Asn  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Thr Val Ser Met Ala Leu Gln Ile Pro Gln Ty  
               
               
                 #r Phe Leu Leu Thr Cys  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Gly Glu Val Val Phe Ser Ile Thr Gly Leu Gl  
               
               
                 #u Phe Ser Tyr Ser Gln  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Ala Pro Ser Asn Met Lys Ser Val Leu Gln Al  
               
               
                 #a Gly Trp Leu Leu Thr  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Val Ala Val Gly Asn Ile Ile Val Leu Ile Va  
               
               
                 #l Ala Gly Ala Gly Gln  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Phe Ser Glu Gln Trp Ala Glu Tyr Val Leu Ph  
               
               
                 #e Ala Ala Leu Leu Leu  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Val Val Cys Ile Ile Phe Ala Ile Met Ala Ar  
               
               
                 #g Phe Tyr Thr Tyr Val  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Asn Pro Ala Glu Ile Glu Ala Gln Phe Asp Gl  
               
               
                 #u Asp Asp Lys Glu Asp  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Asp Leu Glu Lys Ser Asn Pro Tyr Ala Lys Le  
               
               
                 #u Asp Phe Val Ser Gln  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Thr Gln Met  
               
               
                 705  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 18  
               
               
                 &lt;211&gt; LENGTH: 707  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Oryctolagus cuniculus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 18  
               
               
                   
               
               
                 Met Gly Met Ser Lys Ser Leu Ser Cys Phe Gl  
               
               
                 #y Tyr Pro Leu Ser Ile  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Phe Phe Ile Val Val Asn Glu Phe Cys Glu Ar  
               
               
                 #g Phe Ser Tyr Tyr Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Met Arg Ala Leu Leu Ile Leu Tyr Phe Arg As  
               
               
                 #n Phe Ile Gly Trp Asp  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Asp Asn Leu Ser Thr Val Ile Tyr His Thr Ph  
               
               
                 #e Val Ala Leu Cys Tyr  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Leu Thr Pro Ile Leu Gly Ala Leu Ile Ala As  
               
               
                 #p Ala Trp Leu Gly Lys  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Phe Lys Thr Ile Val Trp Leu Ser Ile Val Ty  
               
               
                 #r Thr Ile Gly Gln Ala  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Val Thr Ser Leu Ser Ser Val Asn Glu Leu Th  
               
               
                 #r Asp Asn Asn His Asp  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Gly Thr Pro Asp Ser Leu Pro Val His Val Al  
               
               
                 #a Val Cys Met Ile Gly  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Leu Leu Leu Ile Ala Leu Gly Thr Gly Gly Il  
               
               
                 #e Lys Pro Cys Val Ser  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ala Phe Gly Gly Asp Gln Phe Glu Glu Gly Gl  
               
               
                 #n Glu Lys Gln Arg Asn  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Arg Phe Phe Ser Ile Phe Tyr Leu Ala Ile As  
               
               
                 #n Ala Gly Ser Leu Leu  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Ser Thr Ile Ile Thr Pro Met Val Arg Val Gl  
               
               
                 #n Gln Cys Gly Ile His  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Val Lys Gln Ala Cys Tyr Pro Leu Ala Phe Gl  
               
               
                 #y Ile Pro Ala Ile Leu  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Met Ala Val Ser Leu Ile Val Phe Ile Ile Gl  
               
               
                 #y Ser Gly Met Tyr Lys  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Lys Phe Lys Pro Gln Gly Asn Ile Leu Ser Ly  
               
               
                 #s Val Val Lys Cys Ile  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Cys Phe Ala Ile Lys Asn Arg Phe Arg His Ar  
               
               
                 #g Ser Lys Gln Phe Pro  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Lys Arg Ala His Trp Leu Asp Trp Ala Lys Gl  
               
               
                 #u Lys Tyr Asp Glu Arg  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Leu Ile Ala Gln Ile Lys Met Val Thr Arg Va  
               
               
                 #l Leu Phe Leu Tyr Ile  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Pro Leu Pro Met Phe Trp Ala Leu Phe Asp Gl  
               
               
                 #n Gln Gly Ser Arg Trp  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Thr Leu Gln Ala Thr Thr Met Ser Gly Arg Il  
               
               
                 #e Gly Ile Leu Glu Ile  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Gln Pro Asp Gln Met Gln Thr Val Asn Thr Il  
               
               
                 #e Leu Ile Ile Ile Leu  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Val Pro Ile Met Asp Ala Val Val Tyr Pro Le  
               
               
                 #u Ile Ala Lys Cys Gly  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Leu Asn Phe Thr Ser Leu Lys Lys Met Thr Il  
               
               
                 #e Gly Met Phe Leu Ala  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Ser Met Ala Phe Val Ala Ala Ala Ile Leu Gl  
               
               
                 #n Val Glu Ile Asp Lys  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Thr Leu Pro Val Phe Pro Lys Ala Asn Glu Va  
               
               
                 #l Gln Ile Lys Val Leu  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Asn Val Gly Ser Glu Asn Met Ile Ile Ser Le  
               
               
                 #u Pro Gly Gln Thr Val  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Thr Leu Asn Gln Met Ser Gln Thr Asn Glu Ph  
               
               
                 #e Met Thr Phe Asn Glu  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Asp Thr Leu Thr Ser Ile Asn Ile Thr Ser Gl  
               
               
                 #y Ser Gln Val Thr Met  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Ile Thr Pro Ser Leu Glu Ala Gly Gln Arg Hi  
               
               
                 #s Thr Leu Leu Val Trp  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Ala Pro Asn Asn Tyr Arg Val Val Asn Asp Gl  
               
               
                 #y Leu Thr Gln Lys Ser  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Asp Lys Gly Glu Asn Gly Ile Arg Phe Val As  
               
               
                 #n Thr Tyr Ser Gln Pro  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Ile Asn Val Thr Met Ser Gly Lys Val Tyr Gl  
               
               
                 #u His Ile Ala Ser Tyr  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Asn Ala Ser Glu Tyr Gln Phe Phe Thr Ser Gl  
               
               
                 #y Val Lys Gly Phe Thr  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Val Ser Ser Ala Gly Ile Ser Glu Gln Cys Ar  
               
               
                 #g Arg Asp Phe Glu Ser  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Pro Tyr Leu Glu Phe Gly Ser Ala Tyr Thr Ty  
               
               
                 #r Leu Ile Thr Ser Gln  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Ala Thr Gly Cys Pro Gln Val Thr Glu Phe Gl  
               
               
                 #u Asp Ile Pro Pro Asn  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Thr Met Asn Met Ala Trp Gln Ile Pro Gln Ty  
               
               
                 #r Phe Leu Ile Thr Ser  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Gly Glu Val Val Phe Ser Ile Thr Gly Leu Gl  
               
               
                 #u Phe Ser Tyr Ser Gln  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Ala Pro Ser Asn Met Lys Ser Val Leu Gln As  
               
               
                 #p Arg Trp Leu Leu Thr  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Val Ala Val Gly Asn Ile Ile Val Leu Ile Va  
               
               
                 #l Ala Gly Ala Gly Gln  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Ile Asn Lys Gln Trp Ala Glu Tyr Ile Leu Ph  
               
               
                 #e Ala Ala Leu Leu Leu  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Val Val Cys Val Ile Phe Ala Ile Met Ala Ar  
               
               
                 #g Phe Tyr Thr Tyr Val  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Asn Pro Ala Glu Ile Glu Ala Gln Phe Glu Gl  
               
               
                 #u Asp Glu Lys Lys Lys  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Asn Pro Glu Lys Asn Asp Leu Tyr Pro Ser Va  
               
               
                 #l Ala Pro Val Ser Gln  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Thr Gln Met  
               
               
                 705  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 19  
               
               
                 &lt;211&gt; LENGTH: 714  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Gallus gallus  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 19  
               
               
                   
               
               
                 Met Ala Ala Lys Ser Lys Ser Lys Gly Arg Se  
               
               
                 #r Val Pro Asn Cys Phe  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Gly Tyr Pro Leu Ser Ile Phe Phe Ile Val Il  
               
               
                 #e Asn Glu Phe Cys Glu  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Arg Phe Ser Tyr Tyr Gly Met Arg Ala Val Le  
               
               
                 #u Val Leu Tyr Phe Lys  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Tyr Phe Leu Arg Trp Asp Asp Asn Phe Ser Th  
               
               
                 #r Ala Ile Tyr His Thr  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Phe Val Ala Leu Cys Tyr Leu Thr Pro Ile Le  
               
               
                 #u Gly Ala Leu Ile Ala  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Asp Ser Trp Leu Gly Lys Phe Lys Thr Ile Va  
               
               
                 #l Ser Leu Ser Ile Val  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Tyr Thr Ile Gly Gln Ala Val Met Ala Val Se  
               
               
                 #r Ser Ile Asn Asp Met  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Thr Asp Gln Asn Arg Asp Gly Asn Pro Asp As  
               
               
                 #n Ile Ala Val His Ile  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Ala Leu Ser Met Thr Gly Leu Ile Leu Ile Al  
               
               
                 #a Leu Gly Thr Gly Gly  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ile Lys Pro Cys Val Ser Ala Phe Gly Gly As  
               
               
                 #p Gln Phe Glu Glu His  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Gln Glu Lys Gln Arg Ser Arg Phe Phe Ser Il  
               
               
                 #e Phe Tyr Leu Ser Ile  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Asn Ala Gly Ser Leu Ile Ser Thr Ile Ile Th  
               
               
                 #r Pro Ile Leu Arg Ala  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Gln Glu Cys Gly Ile His Ser Arg Gln Gln Cy  
               
               
                 #s Tyr Pro Leu Ala Phe  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Gly Val Pro Ala Ala Leu Met Ala Val Ser Le  
               
               
                 #u Val Val Phe Ile Ala  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Gly Ser Gly Met Tyr Lys Lys Val Gln Pro Gl  
               
               
                 #n Gly Asn Ile Met Val  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Arg Val Cys Lys Cys Ile Gly Phe Ala Ile Ly  
               
               
                 #s Asn Arg Phe Arg His  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Arg Ser Lys Glu Tyr Pro Lys Arg Glu His Tr  
               
               
                 #p Leu Asp Trp Ala Ser  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Glu Lys Tyr Asp Lys Arg Leu Ile Ala Gln Th  
               
               
                 #r Lys Met Val Leu Lys  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Val Leu Phe Leu Tyr Ile Pro Leu Pro Met Ph  
               
               
                 #e Trp Ala Leu Phe Asp  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Gln Gln Gly Ser Arg Trp Thr Leu Gln Ala Th  
               
               
                 #r Thr Met Asp Gly Asp  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Phe Gly Ala Met Gln Ile Gln Pro Asp Gln Me  
               
               
                 #t Gln Thr Val Asn Pro  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Ile Leu Ile Ile Ile Met Val Pro Val Val As  
               
               
                 #p Ala Val Ile Tyr Pro  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Leu Ile Gln Lys Cys Lys Ile Asn Phe Thr Pr  
               
               
                 #o Leu Arg Arg Ile Thr  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Val Gly Met Phe Leu Ala Gly Leu Ala Phe Va  
               
               
                 #l Ala Ala Ala Leu Leu  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Gln Val Gln Ile Asp Lys Thr Leu Pro Val Ph  
               
               
                 #e Pro Ala Ala Gly Gln  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Ala Gln Ile Lys Ile Ile Asn Leu Gly Asp Se  
               
               
                 #r Asn Ala Asn Val Thr  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Phe Leu Pro Asn Leu Gln Asn Val Thr Val Le  
               
               
                 #u Pro Met Glu Ser Thr  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Gly Tyr Arg Met Phe Glu Ser Ser Gln Leu Ly  
               
               
                 #s Ser Val Met Val Asn  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Phe Gly Ser Glu Ser Arg Ser Glu Asn Ile As  
               
               
                 #p Ser Ile Ser Ser Asn  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Thr His Thr Val Thr Ile Lys Asn Ala Ala Al  
               
               
                 #a Gly Ile Val Ser Ser  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Leu Arg Ser Asp Asn Phe Thr Ser Lys Pro Gl  
               
               
                 #u Glu Gly Lys Asn Leu  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Val Arg Phe Val Asn Asn Leu Pro Gln Thr Va  
               
               
                 #l Asn Ile Thr Met Gly  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 Asp Thr Thr Phe Gly Ile Leu Glu Glu Thr Se  
               
               
                 #r Ile Ser Asn Tyr Ser  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Pro Phe Ser Gly Gly Arg Thr Tyr Asp Ile Va  
               
               
                 #l Ile Thr Ala Gly Ser  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Thr Asn Cys Lys Pro Thr Ser Glu Lys Leu Gl  
               
               
                 #y Tyr Gly Gly Ala Tyr  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Thr Ile Val Ile Asn Glu Cys Ser Gly Asp Va  
               
               
                 #l Thr Gln Leu Arg Tyr  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Ile Glu Asp Ile Gln Pro Asn Thr Val His Me  
               
               
                 #t Ala Trp Gln Ile Pro  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Gln Tyr Phe Ile Leu Thr Cys Gly Glu Val Va  
               
               
                 #l Phe Ser Val Thr Gly  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Leu Glu Phe Ser Tyr Ser Gln Ala Pro Ser As  
               
               
                 #n Met Lys Ser Val Leu  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Gln Ala Gly Trp Leu Leu Thr Val Ala Val Gl  
               
               
                 #y Asn Ile Ile Val Leu  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Ile Val Ala Gly Ala Ser Lys Leu Ser Glu Gl  
               
               
                 #n Trp Ala Glu Tyr Val  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Leu Phe Ala Ala Leu Leu Phe Ala Val Cys Il  
               
               
                 #e Ile Phe Ala Val Met  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Ala Tyr Phe Tyr Thr Tyr Thr Asp Pro Asn Gl  
               
               
                 #u Val Glu Ala Gln Leu  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Asp Glu Glu Glu Lys Lys Lys Gln Ile Lys Gl  
               
               
                 #n Asp Pro Asp Leu His  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Gly Lys Glu Ser Glu Ala Val Ser Gln Met  
               
               
                 705                 7  
               
               
                 #10  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 20  
               
               
                 &lt;211&gt; LENGTH: 2124  
               
               
                 &lt;212&gt; TYPE: DNA  
               
               
                 &lt;213&gt; ORGANISM: Canis familiaris  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 20  
               
               
                   
               
               
                 atgggcatgt ccaagtcata tggttgcttt ggttacccct tgagcatctt ct  
               
               
                 #tcatcgtg     60  
               
               
                   
               
               
                 gtcaatgagt tctgtgaaag attttcctac tatggaatga gagcactcct ga  
               
               
                 #ttctgtac    120  
               
               
                   
               
               
                 ttcagacggt tcatcgggtg ggacgataat ctgtccacgg ccatctacca ca  
               
               
                 #cgtttgtg    180  
               
               
                   
               
               
                 gctctgtgct acctgacgcc gatcctcggc gcactgatcg cagactcctg gc  
               
               
                 #tgggaaag    240  
               
               
                   
               
               
                 ttcaagacaa tcgtgtcact ctccattgtc tacacaattg gacaggcggt ca  
               
               
                 #ctgcagta    300  
               
               
                   
               
               
                 agctcaatta atgacctcac agactataac aaagatggaa ctcctgacaa tc  
               
               
                 #tgtccgtg    360  
               
               
                   
               
               
                 catgtggcac tgtccatgat tggcctggcc ctgatagctc tgggaactgg ag  
               
               
                 #gaataaag    420  
               
               
                   
               
               
                 ccctgtgtgt ctgcatttgg tggagaccag tttgaagagg gccaggaaaa ac  
               
               
                 #aaagaaac    480  
               
               
                   
               
               
                 agattctttt ccatctttta tttggccatt aatgctggaa gcttgatttc ca  
               
               
                 #ctattgtc    540  
               
               
                   
               
               
                 actcccatgc tcagagttca cgaatgtgga atttacagtc agaaagcttg tt  
               
               
                 #acccactg    600  
               
               
                   
               
               
                 gcatttgggg ttcctgctgc tctcatggcc gtatctctga ttgtatttgt ca  
               
               
                 #ttggcagt    660  
               
               
                   
               
               
                 ggaatgtaca agaagtttca gccccagggt aatgtcatgg gtaaagttgt ca  
               
               
                 #agtgcatt    720  
               
               
                   
               
               
                 ggttttgccc tcaaaaatag gtttaggcac cggagtaagc agtttcccaa ga  
               
               
                 #gggagcac    780  
               
               
                   
               
               
                 tggctggact gggctaaaga gaaatacgat gagcggctca tctctcaaat ta  
               
               
                 #agatggtc    840  
               
               
                   
               
               
                 acaaaagtga tgttcttgta catcccactc ccaatgttct gggccctgtt tg  
               
               
                 #accagcag    900  
               
               
                   
               
               
                 ggctccaggt ggacactgca agcaacagct atgagtggga aaattggact tc  
               
               
                 #ttgaagtt    960  
               
               
                   
               
               
                 cagccagatc agatgcagac tgtgaatgcc atcttgattg tcgtcatggt cc  
               
               
                 #ccatcatg   1020  
               
               
                   
               
               
                 gatgccgtgg tgtaccctct gattgcaaaa tgtggcttca atttcacctc ct  
               
               
                 #tgaagagg   1080  
               
               
                   
               
               
                 atgacagttg gaatgttcct ggcttccatg gccttcgtga tggcggcgat tg  
               
               
                 #ttcagctg   1140  
               
               
                   
               
               
                 gaaattgata aaactcttcc agtcttcccc aaacaaaatg aagtccaaat ca  
               
               
                 #aagtactg   1200  
               
               
                   
               
               
                 aatataggaa atggtgccat gaatgtatct tttcctggag cggtggtgac ag  
               
               
                 #ttagccaa   1260  
               
               
                   
               
               
                 atgagtcaat cagatggatt tatgactttt gatgtagaca aactgacaag ta  
               
               
                 #taaacatt   1320  
               
               
                   
               
               
                 tcttccactg gatcaccagt cattccagtg acttataact ttgagcaggg cc  
               
               
                 #atcgccat   1380  
               
               
                   
               
               
                 acccttctag tatgggcccc caataattac cgagtggtaa aggatggcct ta  
               
               
                 #accagaag   1440  
               
               
                   
               
               
                 ccagaaaaag gagaaaatgg aatcagattt ataaatagtc ttaatgagag cc  
               
               
                 #tcaacatc   1500  
               
               
                   
               
               
                 accatgggcg acaaagttta tgtgaatgtc accagtcaca atgccagcga gt  
               
               
                 #atcagttc   1560  
               
               
                   
               
               
                 ttttctttgg gcacaaaaaa cattacaata agttcaacac aacagatctc ac  
               
               
                 #aaaattgt   1620  
               
               
                   
               
               
                 acaaaagttc tccaatcatc caaccttgaa tttggtagtg catataccta tg  
               
               
                 #taatcgga   1680  
               
               
                   
               
               
                 acgcagagca ctggctgccc tgaattgcat atgtttgaag atatttcacc ca  
               
               
                 #acacagtt   1740  
               
               
                   
               
               
                 aacatggctc tgcagatccc gcagtacttc ctcatcacct gcggcgaggt gg  
               
               
                 #ttttctct   1800  
               
               
                   
               
               
                 gtcacaggac tggagttctc atattctcag gccccctcca acatgaagtc gg  
               
               
                 #tgcttcag   1860  
               
               
                   
               
               
                 gcgggatggc tgctgacagt ggctgttggc aacatcattg tgctcattgt gg  
               
               
                 #caggagca   1920  
               
               
                   
               
               
                 ggccagttca gtgaacagtg ggctgaatac atcctatttg cggcattgct tc  
               
               
                 #tggttgtc   1980  
               
               
                   
               
               
                 tgtgtaatat ttgccatcat ggcccggttt tacacttacg tcaatccagc ag  
               
               
                 #agattgaa   2040  
               
               
                   
               
               
                 gctcagtttg acgacgatga gaaaaagaac ctggaaaaga tgaatgtata tt  
               
               
                 #ccacggta   2100  
               
               
                   
               
               
                 actccggtct cacagacaca gatg           
               
               
                 #                   
               
               
                 #              2124  
               
               
                   
               
               
                   
               
               
                 &lt;210&gt; SEQ ID NO 21  
               
               
                 &lt;211&gt; LENGTH: 708  
               
               
                 &lt;212&gt; TYPE: PRT  
               
               
                 &lt;213&gt; ORGANISM: Canis familiaris  
               
               
                   
               
               
                 &lt;400&gt; SEQUENCE: 21  
               
               
                   
               
               
                 Met Gly Met Ser Lys Ser Tyr Gly Cys Phe Gl  
               
               
                 #y Tyr Pro Leu Ser Ile  
               
               
                  1               5   
               
               
                 #                10   
               
               
                 #                15  
               
               
                   
               
               
                 Phe Phe Ile Val Val Asn Glu Phe Cys Glu Ar  
               
               
                 #g Phe Ser Tyr Tyr Gly  
               
               
                             20       
               
               
                 #            25       
               
               
                 #            30  
               
               
                   
               
               
                 Met Arg Ala Leu Leu Ile Leu Tyr Phe Arg Ar  
               
               
                 #g Phe Ile Gly Trp Asp  
               
               
                         35           
               
               
                 #        40           
               
               
                 #        45  
               
               
                   
               
               
                 Asp Asn Leu Ser Thr Ala Ile Tyr His Thr Ph  
               
               
                 #e Val Ala Leu Cys Tyr  
               
               
                     50               
               
               
                 #    55               
               
               
                 #    60  
               
               
                   
               
               
                 Leu Thr Pro Ile Leu Gly Ala Leu Ile Ala As  
               
               
                 #p Ser Trp Leu Gly Lys  
               
               
                 65                   
               
               
                 #70                   
               
               
                 #75                   
               
               
                 #80  
               
               
                   
               
               
                 Phe Lys Thr Ile Val Ser Leu Ser Ile Val Ty  
               
               
                 #r Thr Ile Gly Gln Ala  
               
               
                                 85   
               
               
                 #                90   
               
               
                 #                95  
               
               
                   
               
               
                 Val Thr Ala Val Ser Ser Ile Asn Asp Leu Th  
               
               
                 #r Asp Tyr Asn Lys Asp  
               
               
                             100       
               
               
                 #           105       
               
               
                 #           110  
               
               
                   
               
               
                 Gly Thr Pro Asp Asn Leu Ser Val His Val Al  
               
               
                 #a Leu Ser Met Ile Gly  
               
               
                         115           
               
               
                 #       120           
               
               
                 #       125  
               
               
                   
               
               
                 Leu Ala Leu Ile Ala Leu Gly Thr Gly Gly Il  
               
               
                 #e Lys Pro Cys Val Ser  
               
               
                     130               
               
               
                 #   135               
               
               
                 #   140  
               
               
                   
               
               
                 Ala Phe Gly Gly Asp Gln Phe Glu Glu Gly Gl  
               
               
                 #n Glu Lys Gln Arg Asn  
               
               
                 145                 1  
               
               
                 #50                 1  
               
               
                 #55                 1  
               
               
                 #60  
               
               
                   
               
               
                 Arg Phe Phe Ser Ile Phe Tyr Leu Ala Ile As  
               
               
                 #n Ala Gly Ser Leu Ile  
               
               
                                 165   
               
               
                 #               170   
               
               
                 #               175  
               
               
                   
               
               
                 Ser Thr Ile Val Thr Pro Met Leu Arg Val Hi  
               
               
                 #s Glu Cys Gly Ile Tyr  
               
               
                             180       
               
               
                 #           185       
               
               
                 #           190  
               
               
                   
               
               
                 Ser Gln Lys Ala Cys Tyr Pro Leu Ala Phe Gl  
               
               
                 #y Val Pro Ala Ala Leu  
               
               
                         195           
               
               
                 #       200           
               
               
                 #       205  
               
               
                   
               
               
                 Met Ala Val Ser Leu Ile Val Phe Val Ile Gl  
               
               
                 #y Ser Gly Met Tyr Lys  
               
               
                     210               
               
               
                 #   215               
               
               
                 #   220  
               
               
                   
               
               
                 Lys Phe Gln Pro Gln Gly Asn Val Met Gly Ly  
               
               
                 #s Val Val Lys Cys Ile  
               
               
                 225                 2  
               
               
                 #30                 2  
               
               
                 #35                 2  
               
               
                 #40  
               
               
                   
               
               
                 Gly Phe Ala Leu Lys Asn Arg Phe Arg His Ar  
               
               
                 #g Ser Lys Gln Phe Pro  
               
               
                                 245   
               
               
                 #               250   
               
               
                 #               255  
               
               
                   
               
               
                 Lys Arg Glu His Trp Leu Asp Trp Ala Lys Gl  
               
               
                 #u Lys Tyr Asp Glu Arg  
               
               
                             260       
               
               
                 #           265       
               
               
                 #           270  
               
               
                   
               
               
                 Leu Ile Ser Gln Ile Lys Met Val Thr Lys Va  
               
               
                 #l Met Phe Leu Tyr Ile  
               
               
                         275           
               
               
                 #       280           
               
               
                 #       285  
               
               
                   
               
               
                 Pro Leu Pro Met Phe Trp Ala Leu Phe Asp Gl  
               
               
                 #n Gln Gly Ser Arg Trp  
               
               
                     290               
               
               
                 #   295               
               
               
                 #   300  
               
               
                   
               
               
                 Thr Leu Gln Ala Thr Ala Met Ser Gly Lys Il  
               
               
                 #e Gly Leu Leu Glu Val  
               
               
                 305                 3  
               
               
                 #10                 3  
               
               
                 #15                 3  
               
               
                 #20  
               
               
                   
               
               
                 Gln Pro Asp Gln Met Gln Thr Val Asn Ala Il  
               
               
                 #e Leu Ile Val Val Met  
               
               
                                 325   
               
               
                 #               330   
               
               
                 #               335  
               
               
                   
               
               
                 Val Pro Ile Met Asp Ala Val Val Tyr Pro Le  
               
               
                 #u Ile Ala Lys Cys Gly  
               
               
                             340       
               
               
                 #           345       
               
               
                 #           350  
               
               
                   
               
               
                 Phe Asn Phe Thr Ser Leu Lys Arg Met Thr Va  
               
               
                 #l Gly Met Phe Leu Ala  
               
               
                         355           
               
               
                 #       360           
               
               
                 #       365  
               
               
                   
               
               
                 Ser Met Ala Phe Val Met Ala Ala Ile Val Gl  
               
               
                 #n Leu Glu Ile Asp Lys  
               
               
                     370               
               
               
                 #   375               
               
               
                 #   380  
               
               
                   
               
               
                 Thr Leu Pro Val Phe Pro Lys Gln Asn Glu Va  
               
               
                 #l Gln Ile Lys Val Leu  
               
               
                 385                 3  
               
               
                 #90                 3  
               
               
                 #95                 4  
               
               
                 #00  
               
               
                   
               
               
                 Asn Ile Gly Asn Gly Ala Met Asn Val Ser Ph  
               
               
                 #e Pro Gly Ala Val Val  
               
               
                                 405   
               
               
                 #               410   
               
               
                 #               415  
               
               
                   
               
               
                 Thr Val Ser Gln Met Ser Gln Ser Asp Gly Ph  
               
               
                 #e Met Thr Phe Asp Val  
               
               
                             420       
               
               
                 #           425       
               
               
                 #           430  
               
               
                   
               
               
                 Asp Lys Leu Thr Ser Ile Asn Ile Ser Ser Th  
               
               
                 #r Gly Ser Pro Val Ile  
               
               
                         435           
               
               
                 #       440           
               
               
                 #       445  
               
               
                   
               
               
                 Pro Val Thr Tyr Asn Phe Glu Gln Gly His Ar  
               
               
                 #g His Thr Leu Leu Val  
               
               
                     450               
               
               
                 #   455               
               
               
                 #   460  
               
               
                   
               
               
                 Trp Ala Pro Asn Asn Tyr Arg Val Val Lys As  
               
               
                 #p Gly Leu Asn Gln Lys  
               
               
                 465                 4  
               
               
                 #70                 4  
               
               
                 #75                 4  
               
               
                 #80  
               
               
                   
               
               
                 Pro Glu Lys Gly Glu Asn Gly Ile Arg Phe Il  
               
               
                 #e Asn Ser Leu Asn Glu  
               
               
                                 485   
               
               
                 #               490   
               
               
                 #               495  
               
               
                   
               
               
                 Ser Leu Asn Ile Thr Met Gly Asp Lys Val Ty  
               
               
                 #r Val Asn Val Thr Ser  
               
               
                             500       
               
               
                 #           505       
               
               
                 #           510  
               
               
                   
               
               
                 His Asn Ala Ser Glu Tyr Gln Phe Phe Ser Le  
               
               
                 #u Gly Thr Lys Asn Ile  
               
               
                         515           
               
               
                 #       520           
               
               
                 #       525  
               
               
                   
               
               
                 Thr Ile Ser Ser Thr Gln Gln Ile Ser Gln As  
               
               
                 #n Cys Thr Lys Val Leu  
               
               
                     530               
               
               
                 #   535               
               
               
                 #   540  
               
               
                   
               
               
                 Gln Ser Ser Asn Leu Glu Phe Gly Ser Ala Ty  
               
               
                 #r Thr Tyr Val Ile Gly  
               
               
                 545                 5  
               
               
                 #50                 5  
               
               
                 #55                 5  
               
               
                 #60  
               
               
                   
               
               
                 Thr Gln Ser Thr Gly Cys Pro Glu Leu His Me  
               
               
                 #t Phe Glu Asp Ile Ser  
               
               
                                 565   
               
               
                 #               570   
               
               
                 #               575  
               
               
                   
               
               
                 Pro Asn Thr Val Asn Met Ala Leu Gln Ile Pr  
               
               
                 #o Gln Tyr Phe Leu Ile  
               
               
                             580       
               
               
                 #           585       
               
               
                 #           590  
               
               
                   
               
               
                 Thr Cys Gly Glu Val Val Phe Ser Val Thr Gl  
               
               
                 #y Leu Glu Phe Ser Tyr  
               
               
                         595           
               
               
                 #       600           
               
               
                 #       605  
               
               
                   
               
               
                 Ser Gln Ala Pro Ser Asn Met Lys Ser Val Le  
               
               
                 #u Gln Ala Gly Trp Leu  
               
               
                     610               
               
               
                 #   615               
               
               
                 #   620  
               
               
                   
               
               
                 Leu Thr Val Ala Val Gly Asn Ile Ile Val Le  
               
               
                 #u Ile Val Ala Gly Ala  
               
               
                 625                 6  
               
               
                 #30                 6  
               
               
                 #35                 6  
               
               
                 #40  
               
               
                   
               
               
                 Gly Gln Phe Ser Glu Gln Trp Ala Glu Tyr Il  
               
               
                 #e Leu Phe Ala Ala Leu  
               
               
                                 645   
               
               
                 #               650   
               
               
                 #               655  
               
               
                   
               
               
                 Leu Leu Val Val Cys Val Ile Phe Ala Ile Me  
               
               
                 #t Ala Arg Phe Tyr Thr  
               
               
                             660       
               
               
                 #           665       
               
               
                 #           670  
               
               
                   
               
               
                 Tyr Val Asn Pro Ala Glu Ile Glu Ala Gln Ph  
               
               
                 #e Asp Asp Asp Glu Lys  
               
               
                         675           
               
               
                 #       680           
               
               
                 #       685  
               
               
                   
               
               
                 Lys Asn Leu Glu Lys Met Asn Val Tyr Ser Th  
               
               
                 #r Val Thr Pro Val Ser  
               
               
                     690               
               
               
                 #   695               
               
               
                 #   700  
               
               
                   
               
               
                 Gln Thr Gln Met  
               
               
                 705