Abstract:
A recipe of Chinese herbal composite used in horticulture to kill pests. The practical applications of this recipe include pest killing, germ and virus eradication of vegetables, fruit trees, flowers and economical plants. This recipe is composed of  Gleditisia Sinensis, Sophora flavescens, Aloe vera, Stemona tuberosa, Brucea javanica  and  Dioscorea collettis  vice versa. We prepare it with modern principles and scientific methods to emulsion spray. Those so called modern and scientific are the emulsion processes, micronization, permeability and the multiply potency it possessed. Several laboratory and field test proved that this invention had definite effect on the past killing, germ eradiation and virus inhibition. This invention is belongs to non toxic Chinese herbal preparation. The spray does not harm or cause toxic effect as it touch human skin, eyes, nasal mucosa, lip, mouth and tongue. It does not accumulate in human body and pollute environment.

Description:
BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates generally to medicine, and more particularly to a Chinese herbal composite recipe and its preparation which used in horticulture to kill pests. 
     2. Description of the Related Art 
     The progress in material civilization in these 2 or 3 centuries bring many positive values to human beings. At the same time, there are many severe negative effects appear in ecosystem and environment. The population of the world increased and the horticulture and agriculture plants which feed human must increasing production. Therefore, under the negative changes and effects of the ecosystem, such crops must face the disturbance of disease, toxicity and pest and made it to produce difficultly. 
     The chemical agricultural chemicals propagate, because it&#39;s definitely killing effects and easily usage. It becomes the standby of production in farmers and always the most popular in the market. Until the mid of the twentieth centuries, the techniques of test and examination propagate. The senses of environment and pollution arose and proved gradually that such chemical toxins will remain toxin and pollution. They endanger human health and natural environment. The developed countries prohibit their production domestically. From Jan. 1, 2004, the European Allies will stop selling 320 kind of agricultural chemicals officially and import agricultural side products which polluted by such residues. In fact, the entrepreneurs knew that trend early and devote themselves in production and selling of low toxicity agricultural chemicals. They spent many capitals in research and development and the real effects of such low toxic products are far from those and make the resistance of virus and pest easily. 
     There are many records about agricultural pest killing, germ eradiation and toxin inhibition in the Chinese medical books. The pest killing plants, such as the  Buddleia officinalis, Veratrum mentzeanum, Skimmia japonica, Euphorbia fischeriana  steud,  Sapindus mukorossii  Gaertn, tobacco,  Pyrethrum cineraefolium, Chalcanthite, Stemona tuberosa, Gleditsia sinensis, Melia azedarach  and vice versa. Therefore, the potent Chinese herbs do not according to the specifications of environment protection and sanitation. By the actual experiments, we can kill pests by 6:1 preparations if we can use non toxic Chinese herbs. It shows the conditional situations in economic benefits and usage applications. Therefore, we need some breakthrough in the idea to develop non toxic agricultural chemicals in Chinese herbs. 
     SUMMARY OF THE INVENTION 
     The objective of this invention is to provide a Chinese herbal composite recipe used in horticulture plants to kill pests, eradiate germs and in inhibit viruses. The modern and scientific extraction and preparation methods will augment 6 times and more than it&#39;s original potency. It will open the new applications and values of Chinese medicine. I hope it can replace synthetic agricultural chemicals and maintain agriculture profit, human health and avoid environment pollution and poisoning. 
     To achieve the objectives mentioned above of this invention, I supply the transformation mechanism, drug dispensing, drug preparation method, pharmacology description, range and method of application, caution, transformation and interior experiments and preliminarily field experiments. 
    
    
     DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS 
     The transformation mechanism called in this invention is the mechanism which to achieve more potency. It includes the theories used in traditional Chinese medicine, emulsion and permeability and micronization processes and vice versa. 
     Under the idea of avoiding poisonous Chinese herbs, the potency of organic Chinese herbs is hard to compete with the synthetic agricultural chemicals and pure chemicals. 
     However, it&#39;s principle of application is different to that of the synthetic agricultural chemicals and pure chemicals. It is not based on poison killing principle but on the theories of traditional Chinese medicine not to harm the body to eradiate germs or killing Ascarid, tape worm, hook worm, Nematode and inhibit poisons to heal the disease. 
     To increase potency on the original statement, the inventor analyzed the effective components of these drugs especially. To make them have multiply effects in assemble and preparation. I take reasonable concentration extraction to avoid the losses of alkaloids and necessary ingredients. Especially, I take more advanced and scientific grinding and colloid milling machine than original preparation method to perform micronized and special emulsion process. 
     The effects of pesticide, germicide and toxin inhibition of Chinese herbs is to promote drug entered from the derma and mucosa into the bodies of warms, this is the first step. This will cause the environment inside the body and the tissues change dramatically. To achieve such goals, the most important thing is the micronization of drugs and it&#39;s permeability except the composition of drugs and it does multiply effects. Therefore, this preparation uses some emulsion process to make water soluble emulsion because of the water soluble emulsion is easily absorbed by skin and mucosa. 
     The tension between liquid and gas is called surface tension. The liquids which do not intermingle each other, like the Mosla chinensis oil used in this preparation (the main ingredients are thymol, carvacreol and p-cymene), turpentine oil and the tension between the percolate is called the surface tension. The emulsified agents like yellow gelatin, Tragacantha, gum Arabic are belonging to surface active agents. They can reduce the surface tension between oil and percolate and down to 10 dyne/cm. The objective of emulsion is to make the oily effective ingredients into small droplets by emulsion agents and colloidal mill which supply energy from the outside world. That will make it micronized and more easily penetrate into worm bodies and to increase the absorption rate by derma and mucosa of worms and germs. That will change the interior environ of germs and worms dramatically. 
     As mentioned above, this is the mechanism of drug achieve effect and multiply potency. To prove this point of mechanism, the inventor performs the interior pest killing experiments (see interior experiment I) of transformation mechanism. That proved the turnover effect with emulsion added. 
     The Chinese compose recipe spray used in horticulture is prepared by Chinese herbs listed below, then I concentrated extraction and make powder by ratio with some definitely ratio percolate. Then I pulverized it by grinding and sieving with emulsified agents added.
     (1) Concentrated Chinese powder and ratio.
         Gleditsia sinensis  80-120 gm  Stemona tuberosa  80-120 gm     Sophora flavescens  80-120 gm  Brucea iavanica  80-120 gm     Aloe vera  80-120 gm   
       (2) Percolate and ratio.
       distilled water 600-800 ml alcohol 150-250 ml   glycerin 80-120 ml   
       (3) Emulsified agents used in original 3000ml of solution.
       Yellow gelatin or Gum arabic 40-60gm.   Turpentine oil 40-60 ml.   Distilled  Mosla chinensis  oil 30-50 ml.
 
Preparation
   (1) Take concentrated Chinese composite recipe 1000 gm then passed milling machine for 2 to 3 times and soak for 2 hours. The first time percolate is 1200 ml. The second percolate is 1000 ml after soaking for 2 hours. Add distilled water 1200 ml and soak for 4 hours then percolate to get the third percolate. Combine these three percolates for later use.   (2) Take yellow gelatin or gum Arabic 50 gm and mix turpentine oil and  Mosla chinensis  oil 50 ml, respectively. Put them into colloid mill for high speed grinding than add distilled water 100 ml. Continue grinding for 3 to 5 minutes and repeat for 3 times.   (3) Adding 50 gm of Mirabilite and 3 gm of benzoic acid. Mixing them with (1) and (2). Passing them in golden mill and grinding 2 to 3 times.
 
Pharmacology
   1 . Gleditsia sinensis : Pesticide, germicide, toxin inhibition; contain gledinin and alkanes. It will increase permeability with other drugs.   
       

     2 . Sophora flavescens : It has broad spectrum germicide effect and can help other drugs to kill pests.
         3 . Aloe vera : Pesticide, germicide, the lauric acid and myristic acid is helpful for mirabilite to promote solution penetration.   4 . Stemona tuberosa : Anti-microbe, pesticide, killing flied and mosquito and other 10 or more agricultural pests.   5 . Brucea javanica : Pesticide. The effect is enhanced by the gledinin and sodium sulfate (Mirabilite).   6 . Dioscorea collettii : Germicide and pesticide. The important ingredient is saponin unit.   7 . Mosla chinensis : It is extracted with distilled method. It contains pesticide and germicidal effects of essential oils like carvacrol, thymol and p-cymene. They make this preparation more delicate. I use it as the emulsion oil and make micronized material accomplish the assumed effect.   8. Turpentine oil: Germ resistance, germicide; emulsion oil.   9. Yellow gelatin: Emulsifying agent, protect plants.   10. Mirabilite: Working with other drugs to produce help and multiply effects to increase permeability and achieve pest and bacteria killing effects. (Please see drug description sheet for detail information.)
 
Range and Method of Application
       1. This spray is applicable to economic crops, fruit trees, flowers, vegetables Lima bean. It also applies to general agricultural damages caused by pests, germs and viruses on crops.   2. Take this spray and dilute to certain ratios of water then spray on stems and leaves of crops or pests by manual or mechanical sprayer.   3. Depend on the order and families of pests, germs and viruses and the severity to determinate the dilution ratio.
       Dilute 15 to 30 times for every pests, germs and toxins.   Dilute 30 to 60 times need 24 to 48 hours to kill or repel them.   Dilute 60 to 150 times has prevention effect.   
       4. The preparation is a contact pesticide. It shows eradiation effect on larva, adult and eggs. These pest and diseases can not produce resistance to this preparation. It do not block environ biological chains. You do not have to increase dilution ratio gradually. The prevention period can be extended. It is harmless to animals and human.
 
Caution
   1. This invention is a topically Chinese medicine pest and germ killing agent. It is prohibited to take orally. It may cause gastrointestinal symptoms when take orally. You can purchase rare green bean powder, rare glycyrrhizin powder and talcum powder 6 gm, respectively. Mixing 6 gm of cold water with these things and take it. It will show some laxative effect or you should consult medical assistance.   2. The diluents of this preparation are harmless to animals and human. It do not cause any harm as contact with skin, eyes, nose, mouth and tongue.   3. This preparation should store in cool and dry places to prevent deterioration caused by high temperature.
 
Addendum
   1. This horticultural Chinese composite spray is a quantitized concentrated Chinese extract powder. It is prepared by certain ingredients and ratio of percolate then dissolved in special emulsified agents.   2. This invention is the best practice of the Chinese composite recipe shown above. It can produce sufficient effect without adding  Aloe . On the other side, the extraction of this recipe all using  Dioscorea collettii  powder as adjuvant but other drugs will do, too.   3. The feature of this preparation&#39;s re-extraction percolate is composed of the following ration of liquids.   4. Distilled water 60-80%, alcohol 15-25 % and glycerin 8-12 %.   5. The feature of this preparation is the emulsion process. It adds yellow gelatin   6. The other feature of this invention is adding 30 to 120 gm of Mirabilite (sodium sulfate) in to 3000 ml of original solution.   7. The feature of this preparation retains the right as application of environmental sanitation. That is to say, this invention and feature of preparation can be sprayed on the living environment to keep the sanitation of environ.
 
Drug Description Datasheet
   

     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 1 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Zaolia 
                 Official name 
                 
                   Gleditsia sinensis 
                 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume IV pp 480 
               
               
                   
                 (China Sheng-Ghai scientific technology 
               
               
                   
                 publication) 
               
               
                 Growing district, 
                 Grow in northeast, north south of China and 
               
               
                 collect season, 
                 Si-chuan, Qui-zhou province. Collect fruits 
               
               
                 parts 
                 and dry as they ripen and darken in autumn. 
               
               
                 Preparation, 
                 (1) Take 12 kg of  Gleditsia sinensis  and 
               
               
                 extraction process, 
                 soak in water for 6 hours. Collect percolate 
               
               
                 method 
                 for 2 times then evaporate the residue water to 
               
               
                   
                 get 5 liters of fluid extract. Adding 2 kg 
               
               
                   
                   Dioscorea collettii  of powder and stir well. 
               
               
                   
                 Drying it in 45° C. then pulverized by passing 
               
               
                   
                 #120 sieve to make fine powder. Collect and 
               
               
                   
                 store them for later use. 
               
               
                 Effective 
                 The capsules contain tri-terpene saponin that is 
               
               
                 ingredient 
                 gledinin saponin. Its basic saponin unit is 
               
               
                   
                 gledinin, gledinin saponin, wax alcohol, 
               
               
                   
                 novacosane, nor-septacosane, stigmasterol, 
               
               
                   
                 sitosterol and tannin. 
               
               
                 Original recorded 
                 Germicide effect. The  Gleditsia sinensis  extract 
               
               
                 pharmacological 
                 show fungus inhibition effect on  T. violaceum   
               
               
                 effects 
                 and  Norcardis asteroids  in skin.  Fructus   
               
               
                   
                   Gleditsiae abnormalis  can kill larva of 
               
               
                   
                 nematode and show hemolysis in vitro. The 
               
               
                   
                 main feature is local mucosa stimulation effect. 
               
               
                   
                 In central system, it can cause them convulsion 
               
               
                   
                 and paralysis then death with breathe 
               
               
                   
                 exhaustion. 
               
               
                 Pharmacological 
                 Pesticide and germicide objectives are achieved 
               
               
                 applications, 
                 by the ingredients contain in the  Gleditsia   
               
               
                 purposes 
                   sinensis  such as saponins, alkanes and alcohols. 
               
               
                   
                 They can cooperate with other drugs to 
               
               
                   
                 promote penetration effect. 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 2 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Kushen 
                 Official name 
                 
                   Sophora flavescens 
                 
               
               
                   
                   
                   
                 Ait 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume IV pp 634 (China 
               
               
                   
                 Sheng-Ghai scientific technology publication) 
               
               
                 Growing district, 
                 Grow in everywhere in China. Take all plants in 
               
               
                 collect season, 
                 September to October and remove the upper 
               
               
                 parts 
                 part, base of stem and fibrous roots. Cut fibrous 
               
               
                   
                 roots into single strand. Wash out dirt then dry or 
               
               
                   
                 bake it. 
               
               
                 Preparation, 
                 Take 12 kg of  Sophora flavescens  and soak in 
               
               
                 extraction process, 
                 water for 3 hours. Collect percolate for 2 times 
               
               
                 method 
                 then evaporate the residue water to get 5 liters of 
               
               
                   
                 fluid extract. Adding 2 kg  Dioscorea collettii  of 
               
               
                   
                 powder and stir well. Drying it in 60° C. then 
               
               
                   
                 pulverized by passing #120 sieve to make fine 
               
               
                   
                 powder. Collect and store them for later use. 
               
               
                 Effective 
                 The roots contain 13 alkaloids including matrine 
               
               
                 ingredient 
                 and many flavonoids mixtures. Otherwise, the 
               
               
                   
                 roots contain tri-terpene saponin, soy saponin and 
               
               
                   
                 kurarinone. 
               
               
                 Original recorded 
                 The in vitro experiments show that 1% matrine 
               
               
                 pharmacological 
                 has apparent bacteria inhibition effects on  
               
               
                 effects 
                 Shigella,  E. Coli , Proteus,  B-streptococcus  and 
               
               
                   
                   Staphylococcus aureaus . The total flavones of 
               
               
                   
                   Sophora flavescens  and their ethanol extract have 
               
               
                   
                 killing or growth inhibition effects on  Giardia   
               
               
                   
                   lamblia  Stiles in vitro. 
               
               
                 Pharmacological 
                 It can use specific alkaloids and saponins to 
               
               
                 applications, 
                 achieve goals of killing pests and bacteria. The 
               
               
                 purposes 
                 inventor observed 20 concentrated Chinese 
               
               
                   
                 medicine drugs powder all using starch as 
               
               
                   
                 adjuvant for 20 years and found that the  Sophora   
               
               
                   
                   flavescens  did not eat into by worms and not 
               
               
                   
                 change its quality. The package still remains 
               
               
                   
                 intact. It shows the effect of germicide. 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 3 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Luhui 
                 Official name 
                   Aloe vera  L. var chinensis 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume VIII pp 51 (China 
               
               
                   
                 Sheng-Ghai scientific technology publication) 
               
               
                 Growing district, 
                 Grow in Fu-jiann, Taiwan, Kuang-tong, Si-chuan, 
               
               
                 collect season, 
                 Yun-nan. Gather the middle and lower parts of 
               
               
                 parts 
                 leaves by batches separately. Cut the leaves open 
               
               
                   
                 and collect liquids then drying it. The other way is 
               
               
                   
                 to cut it and add the same part of water then boiling 
               
               
                   
                 for 2 to 3 hours. Filtering, concentrating and drying. 
               
               
                 Preparation, 
                 Purchase pure and clean Aloe concentrate blocks in 
               
               
                 extraction process, 
                 the market. Pulverized before use. 
               
               
                 method 
               
               
                 Effective 
                 The leaves contain 11 acids such as Alesin, Aloe 
               
               
                 ingredient 
                 tannin, lauric acid, myristic acid and vice versa. 
               
               
                   
                 There are some polysaccharides and sugar polymers. 
               
               
                 Original recorded 
                 Pesticide, germicide. 
               
               
                 pharmacological 
               
               
                 effects 
               
               
                 Pharmacological 
                 Lauric acid and the sodium sulfate in the Mirabilite 
               
               
                 applications, 
                 can cooperative together to increase penetration 
               
               
                 purposes 
                 ability into worm body. 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 4 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Baibu 
                 Official name 
                   Stemona tuberose  Lour 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume VIII pp 189 (China 
               
               
                   
                 Sheng-Ghai scientific technology publication) 
               
               
                 Growing district, 
                   Stemona tuberose  grows in southeast province of 
               
               
                 collect season, 
                 China, Hu-nan, Hu-peh and Si-chuan. Collect them 
               
               
                 parts 
                 after 3 years of planting. Remove tubers after 
               
               
                   
                 wilting of upper parts of shoot before spring. 
               
               
                   
                 Remove fine roots and boiling in hot water to 
               
               
                   
                 well done then drying or baking them. 
               
               
                 Preparation, 
                 Take 12 kg of  Sophora flavescens and soak in   
               
               
                 extraction process, 
                 water for 3 hours. Collect percolate for 2 times 
               
               
                 method 
                 then evaporate the residue water to get 5 liters of 
               
               
                   
                 fluid extract. Adding 2 kg  Dioscorea collettii  of 
               
               
                   
                 powder and stir well. Drying it in 60° C. then 
               
               
                   
                 pulverized by passing #120 sieve to make fine 
               
               
                   
                 powder. Collect and store them for later use. 
               
               
                 Effective 
                 The  Stemona tuberose  contains 13 alkaloids 
               
               
                 ingredient 
                 including syemonine, tuberostemonine and 
               
               
                   
                 isotuberostemonine. It also contains sugar, fats, 
               
               
                   
                 proteins, ash and 6 different acids including oxalic 
               
               
                   
                 acid. 
               
               
                 Original recorded 
                 1. Anti-microbe. 
               
               
                 pharmacological 
                 2. Anti-parasite: In vitro tests show that 50% 
               
               
                 effects 
                 solution can kill pin worm in 20 hours. The 
               
               
                   
                 tuberostemonine can paralyze Ascarid. 
               
               
                   
                 3. Insect killing effects: The  Stemona tuberose   
               
               
                   
                 water solution and alcohol solution has effects on 
               
               
                   
                 lice and cause eggs of lice not hatch. It also show 
               
               
                   
                 contact killing effect on 10 or more pests including 
               
               
                   
                 maggot, larva of mosquito, bed bug, aphids, larva 
               
               
                   
                 of  Chilo simples  and spider. 
               
               
                 Pharmacological 
                 Pesticide, germicide and anti-viral effects. 
               
               
                 applications, 
               
               
                 purposes 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 5 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Yadanzi 
                 Official name 
                 
                   Brucea jananica 
                 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume V pp7 (China 
               
               
                   
                 Sheng-Ghai scientific technology publication) 
               
               
                 Growing district, 
                 Grow mainly in Fu-kien, Taiwan, Hai-nan, Kwang- 
               
               
                 collect season, 
                 dong, Kwang-si, Yun-nan and Kwei-chow. The 
               
               
                 parts 
                 fruits ripen in autumn. Collect fruits as the skin of 
               
               
                   
                 fruits darken then cleaning and drying. 
               
               
                 Preparation, 
                 Take 12 kg of  Brucea jananica  and soak in water 
               
               
                 extraction process, 
                 for 6 hours. Collect percolate for 2 times then 
               
               
                 method 
                 evaporate the residue water to get 5 liters of fluid 
               
               
                   
                 extract. Adding 2 kg  Dioscorea collettii  of powder 
               
               
                   
                 and stir well. Drying it in 45° C. then pulverized by 
               
               
                   
                 passing #120 sieve to make fine powder. Collect and 
               
               
                   
                 store them for later use. 
               
               
                 Effective 
                 It contains more than 30 ingredients which structural 
               
               
                 ingredient 
                 similar to bitter ingredients of quassin. There are 
               
               
                   
                 several glycosides including lignan of  Hemerocallis   
               
               
                   
                   fulva , vanillic acid and  Hypericum . It also contains 
               
               
                   
                 oleic acid, triglyceride and oil of  Brucea jananica . 
               
               
                 Original recorded 
                 Anti-malaria and kill plasmodium and amoeba. 
               
               
                 pharmacological 
                 The crude extract can repel nematodes and tape 
               
               
                 effects 
                 worms in large intestine. It also has repelling effects 
               
               
                   
                 on pin worm and Ascarid. 
               
               
                 Pharmacological 
                 It shows multiply effects with  Mirabilite  and 
               
               
                 applications, 
                   Gleditisia sinensis  and demonstrates the features 
               
               
                 purposes 
                 pesticide effect. 
               
               
                   
               
             
          
         
       
     
                                                         NO: 6                                Name   Beixie   Official name     Discorea collettii  Hook                    Dependence   China Pharmacopoeia Volume VIII pp 231 (China           Sheng-Ghai scientific technology publication)       Growing district,   Grow mainly in Che-kiang, Anh-wei, Jiang-si, Hu-       collect season,   nan. Digging the root stem in autumn and winter       parts   then remove the fibrous roots. Cleaning the dirt and           slice for druying.       Preparation,   Take 10 kg of  Discorea collettii  and pulverized by       extraction process,   passing #100 sieves to make fine powder as the       method   adjuvant of collecting fluid extracts.       Effective   It contains 9 steroids. Dios - genin, Yamogenin, Δ       ingredient   3,5-deoxy-tigogenin, Δ3,5-deoxy-neotigogenin,           Diosgenin palmitate, Yamogenin palmitate,           β-Sitoterol, a pair of Epimerides and two tzi-gan.       Original recorded   The water extract has insect killing effects. It shows       pharmacological   effective ingredients is  discorea saponin . It has       effects   anti-fungal effect to Trichophyton and  Giardia               lamblia  but no effect to bacteria. The  Dioscorea               collettii  has the effect of fish poison but not           apparently as tested.       Pharmacological   Pesticide and as adjuvant.       applications,       purposes                    
Drug Description Datasheet
 
     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 7 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Howgining 
                 Official name 
                 
                   Mosla 
                 
               
               
                   
                   
                   
                   chinensis  Maxim. 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume VII pp 91 (China 
               
               
                   
                 Shang-Ghai scientific technology publication) 
               
               
                 Growing district, 
                 It grows in east China, Taiwan, Kwei-chow. The 
               
               
                 collect season, 
                 flowers bloom in autumn and winter. Collect it as 
               
               
                 parts 
                 the stems and leaves prosper. Drying under shade 
               
               
                   
                 and pack into small bundles. 
               
               
                 Preparation, 
                 Take 12 kg of  Mosla chinensis  and chop into fine 
               
               
                 extraction process, 
                 stripes. Place them into distillation barrel and press 
               
               
                 method 
                 firmly. Using heat to perform partial distillation in 
               
               
                   
                 order to get the essential oil about 240 ml. Remove 
               
               
                   
                 the remainder and store the essential oil in the 
               
               
                   
                 bottle for later use. 
               
               
                 Effective 
                 The  Mosla chinensis  is called the Shi-xiang-ru. The 
               
               
                 ingredient 
                 total plant contains volatile oil 2% including 
               
               
                   
                 carvacrol 71.64%, p-cymene 10.10%, p-isopropyl 
               
               
                   
                 phenyl methyl alcohol 5%, β-pinene 1.52%, 
               
               
                   
                 terpineol 1.23%, thymol 1.40%, caryophyllene 
               
               
                   
                 1.36%, β-farnesene 0.25%, limonene 0.15%. 
               
               
                 Original recorded 
                 The  Mosla chinensis  volatile oil possesses stronger 
               
               
                 pharmacological 
                 broad spectrum germicide effect. The main 
               
               
                 effects 
                 effective anti-bacterial ingredients are thymol, 
               
               
                   
                 carvacrol and p-cymene. 
               
               
                 Pharmacological 
                 Used as emulsifying oil. It can potentiate the 
               
               
                 applications, 
                 pesticide, germicide potency of essential oils and  
               
               
                 purposes 
                 bring other pest and germ killing agents&#39; potency. 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 8 Emulsifying oil 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Songlievou 
                 Official name 
                 Pinus oil 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume II pp 305 (China 
               
               
                   
                 Shang-Ghai scientific technology publication) 
               
               
                 Growing district, 
                 It grows mainly in Kwang-dong and other 
               
               
                 collect season, 
                 provinces. We get the volatile oils distilled or 
               
               
                 parts 
                 extracted from the oily resin. 
               
               
                 Preparation, 
                 Purchase authentic pure and clean turpentine for 
               
               
                 extraction process, 
                 later use. 
               
               
                 method 
               
               
                 Effective 
                 It contains resin acids, fatty acids, mono-terpines 
               
               
                 ingredient 
                 and semi-terpines 
               
               
                 Original recorded 
                 1. Anti-microbe effect. In vitro experiments show 
               
               
                 pharmacological 
                 stronger anti-fungal effects ( Candida albicans ) on 
               
               
                 effects 
                   Tinea corporis  and dermatophytosis It has certain 
               
               
                   
                 degree of germ inhibition effects on  Staphylococcus   
               
               
                   
                   aureaus  and  E. Coli . It is used ad emulsifying oil 
               
               
                   
                 in this preparation. 
               
               
                 Pharmacological 
                 Emulsifying agent, germicide and parasite repelling. 
               
               
                 applications, 
               
               
                 purposes 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 9 Emulsifying agent 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Yellow gelatin 
                 Official 
                 
                   Colla corii 
                 
               
               
                   
                   
                 name 
                 Bon&#39;s 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume IX pp 680 (China 
               
               
                   
                 Sheng-Ghai scientific technology publication) 
               
               
                 Growing district, 
                 Chop dried yellow ox leather into small cubes. 
               
               
                 collect season, 
                 Adding them into water and soak for 2 days. You 
               
               
                 parts 
                 must change water and stirring well frequently. 
               
               
                   
                 Cleaning it as the leather soften and put into copper 
               
               
                   
                 pot. Boiling it gently with 5 times of water. Adding 
               
               
                   
                 water frequently and filtering supernatant for every 
               
               
                   
                 24 hours. Repeat these for 3 times. Precipitate the 
               
               
                   
                 supernatant with alunite and pour the supernatant. 
               
               
                   
                 Re-heat and condense in the copper pot till the 
               
               
                   
                 liquid do not absorb by filtering paper. Adding some 
               
               
                   
                 yellow wine or candy sugar to collect the gelatin. 
               
               
                   
                 Pour it into the plate and cool. Chop it into small 
               
               
                   
                 pieces for drying. 
               
               
                 Preparation, 
                 Take pure clear yellow gelatin from market and 
               
               
                 extraction process, 
                 pulverized into powder. 
               
               
                 method 
               
               
                 Effective 
                 Collagen in the leather of the yellow ox is not  
               
               
                 ingredient 
                 soluble in water. Boiling with water for a long 
               
               
                   
                 period can transform collagen into water soluble 
               
               
                   
                 yellow gelatin. It contains nitrogen, sugar, multiple 
               
               
                   
                 vitamins and helpful to plant growth. 
               
               
                 Original recorded 
               
               
                 pharmacological 
               
               
                 effects 
               
               
                 Pharmacological 
                 Promote growth of plants. It is used as emulsifying 
               
               
                 applications, 
                 agent in this invention. 
               
               
                 purposes 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
             
           
               
                   
               
               
                 NO: 10 Agumenter 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Name 
                 Puxiao 
                 Official name 
                 Mirabilite 
               
               
                   
               
             
          
           
               
                 Dependence 
                 China Pharmacopoeia Volume I pp 269 (China 
               
               
                   
                 Shang-Ghai scientific technology publication) 
               
               
                 Growing district, 
               
               
                 collect season, 
               
               
                 parts 
               
               
                 Preparation, 
                 Purchase authentic Mirabilite for later use. 
               
               
                 extraction process, 
               
               
                 method 
               
               
                 Effective 
                 Hydrated sodium sulfate (Na2SO4-10H2O) mainly  
               
               
                 ingredient 
                 with trace sodium chloride and inorganic elements 
               
               
                   
                 such as calcium, magnesium and potassium. Use it 
               
               
                   
                 to penetrate and soften. 
               
               
                 Original recorded 
               
               
                 pharmacological 
               
               
                 effects 
               
               
                 Pharmacological 
                 To produce cooperative or additional effect with 
               
               
                 applications, 
                 drugs such as  Aloe vera ,  Gleditsia sinensis , 
               
               
                 purposes 
                   Brucea javanica  and vice versa. It can promote 
               
               
                   
                 pesticide, germicide and penetration effects. 
               
               
                   
               
             
          
         
       
     
     Field experiment example I Notolophus  Australia posticus, Chlorita flavescens  and tea red spider in tea tree farm. 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Objective of experiment 
                 The effect of this invention 
               
               
                   
                   
                 is applied to the pest in the 
               
               
                   
                   
                 tree farm. 
               
               
                   
                 Time and place 
                 Apr. 13, 2003. #1123 in 
               
               
                   
                   
                 Xin-kai Section, Liu-gui, 
               
               
                   
                   
                 Kaohsiung county. 
               
               
                   
                 Environment 
                 There are 20 acres of dry 
               
               
                   
                   
                 land with elevation of 800 
               
               
                   
                   
                 meters. Tea trees grow in 
               
               
                   
                   
                 1983 then betel trees in 1990. 
               
               
                   
                 Status of 
                 1. Many diverse of tea trees 
               
               
                   
                 pest damage 
                 and back of old leaves found 
               
               
                   
                   
                 larva of red spiders and 
               
               
                   
                   
                 some old leaves fallen. 
               
               
                   
                   
                 2. The fresh leaves have 
               
               
                   
                   
                 bite wounds. Shaking the bush 
               
               
                   
                   
                 and found  Chlorita flavescens   
               
               
                   
                   
                 flying everywhere. The 
               
               
                   
                   
                 situation is getting worse. 
               
               
                   
                   
                 3. The down-left area of this 
               
               
                   
                   
                 tea tree farm appear larva of 
               
               
                   
                   
                   Notolophus Australia posticus . 
               
               
                   
                 Pest name 
                 1. Tea red spider is called 
               
               
                   
                 and brief 
                 tea mite. It&#39;s very tiny. The 
               
               
                   
                 description 
                 female is ellipse shaped and 
               
               
                   
                   
                 length is 0.34 mm. The male 
               
               
                   
                   
                 one is small shield shaped. 
               
               
                   
                   
                 The color is purple-red or 
               
               
                   
                   
                 deep red. The color in the 
               
               
                   
                   
                 back is faint. There are loose 
               
               
                   
                   
                 white fine hairs in the body. 
               
               
                   
                   
                 The legs are pale-red color. 
               
               
                   
                   
                 The larvas have 3 pairs of les 
               
               
                   
                   
                 and the adult has 4 pairs of 
               
               
                   
                   
                 legs. They damage old tea tree 
               
               
                   
                   
                 leaves and make them 
               
               
                   
                   
                 wither and fall. 
               
               
                   
                   
                 2.  Chlorita flavescens  is 
               
               
                   
                   
                 called tea cicada. It is fragile. 
               
               
                   
                   
                 The body length is 1 to 3 
               
               
                   
                   
                 mm with pale green and 
               
               
                   
                   
                 transparent body. The fore- 
               
               
                   
                   
                 wing is yellow only. They 
               
               
                   
                   
                 suck the juice of fresh leaves 
               
               
                   
                   
                 and block the development 
               
               
                   
                   
                 of tea tree leaves. The damages 
               
               
                   
                   
                 leaves are shrinking with 
               
               
                   
                   
                 faint-yellow color and the 
               
               
                   
                   
                 rim of leaves become brown. 
               
               
                   
                   
                 The leaves scroll into 
               
               
                   
                   
                 ship shape and fallen. 
               
               
                   
                   
                 3.  Notolophus Australia   
               
               
                   
                   
                   posticus  is called red-head 
               
               
                   
                   
                 poison caterpillar or stinky 
               
               
                   
                   
                 dog. The body length is 22 to 
               
               
                   
                   
                 30 mm. The body color is 
               
               
                   
                   
                 variable depend on seasons. In 
               
               
                   
                   
                 summer, the head is red and 
               
               
                   
                   
                 the body is in light yellow. 
               
               
                   
                   
                 There are may yellow and 
               
               
                   
                   
                 white long hairs all over the 
               
               
                   
                   
                 worm body. It will cause 
               
               
                   
                   
                 allergic itch and pain as 
               
               
                   
                   
                 touched human skin. They eat 
               
               
                   
                   
                 leaves of tea trees and 
               
               
                   
                   
                 damage severely in March to May. 
               
               
                   
                 General 
                 Use 100 times of water with 
               
               
                   
                 status of 
                 1 part of this preparation. 
               
               
                   
                 application 
                 Apply it with mobile sprayer. 
               
               
                   
                   
                 There are about 2000 tea 
               
               
                   
                   
                 trees. The original solution 
               
               
                   
                   
                 of this preparation used is 3 
               
               
                   
                   
                 liters and 157 liters of water. 
               
               
                   
                 Effect 
                 Inspect at afternoon of Apr. 
               
               
                   
                 evaluation 
                 14, 2004. There are no tea 
               
               
                   
                   
                 mites found and no  Chlorita   
               
               
                   
                   
                   flavescens  flying as shaking 
               
               
                   
                   
                 bushes. The  Notolophus Australia   
               
               
                   
                   
                   posticus  died in the ground. 
               
               
                   
                   
                 Inspect at afternoon of Apr. 
               
               
                   
                   
                 20, 2004. The shoots of tea 
               
               
                   
                   
                 trees grow normally and no 
               
               
                   
                   
                 pests seen. 
               
               
                   
                 postscript 
                 Actually, from the beginning 
               
               
                   
                   
                 of this tea tree farm 
               
               
                   
                   
                 opened. I always using 
               
               
                   
                   
                 Chinese medicine powder to 
               
               
                   
                   
                 dilute 150 times and proved 
               
               
                   
                   
                 effective. Now, I improved 
               
               
                   
                   
                 it into emulsion and dilute 
               
               
                   
                   
                 into 100 times and the effect 
               
               
                   
                   
                 more apparently. 
               
               
                   
                   
               
             
          
         
       
     
     Field experiment example II  Pesudaulacaspis pentagonai  in magnolia tree. 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Objective of 
                 The effects of this invention 
               
               
                   
                 experiment 
                 apply to the  Pesudaulacaspis   
               
               
                   
                   
                   pentagonai  in magnolia tree. 
               
               
                   
                 Time and 
                 Nov. 24, 2003. #1123 in 
               
               
                   
                 place 
                 Xin-kai Section, Liu-qui, 
               
               
                   
                   
                 Kaohsiung county. 
               
               
                   
                 Environment 
                 The magnolia tree is 20 years 
               
               
                   
                   
                 old and grew in front of the 
               
               
                   
                   
                 house. 
               
               
                   
                 Status of 
                 There are 2 magnolia trees 
               
               
                   
                 pest damage 
                 in these experiments. They 
               
               
                   
                   
                 have damaged by  Pesudaulacaspis   
               
               
                   
                   
                   pentagonai  for many 
               
               
                   
                   
                 years. The middle sections 
               
               
                   
                   
                 of the stem always show 
               
               
                   
                   
                 gray-white appearance. This 
               
               
                   
                   
                 appearance may extend to the 
               
               
                   
                   
                 twig and make the upper part 
               
               
                   
                   
                 green leaves of the damaged 
               
               
                   
                   
                 section withering. The blooms 
               
               
                   
                   
                 are weak and small. The 
               
               
                   
                   
                 twigs died at last. The trees 
               
               
                   
                   
                 is so big and good drainage 
               
               
                   
                   
                 and fresh air make them live 
               
               
                   
                   
                 till today. I can fell the 
               
               
                   
                   
                 humidity of larva and odor as 
               
               
                   
                   
                 touch the surface of the stem 
               
               
                   
                   
                 with hands. 
               
               
                   
                 Pest name 
                 
                   Pesudaulacaspis pentagonai 
                 
               
               
                   
                 and brief 
                 Targioni-Tozzett is hosted on 
               
               
                   
                 description 
                   Melia azedarach , prune, plum, 
               
               
                   
                   
                 peach, almond tree, apple 
               
               
                   
                   
                 tree, magnolia tree and 
               
               
                   
                   
                   Hibiiscus mutabilis . The worms 
               
               
                   
                   
                 attach itself on the base of 
               
               
                   
                   
                 the stems first. The damages 
               
               
                   
                   
                 skin form gray-white or powder 
               
               
                   
                   
                 like sign. The larva and 
               
               
                   
                   
                 female suck juice and extend 
               
               
                   
                   
                 to the tip of twigs. The 
               
               
                   
                   
                 damaged twigs withering, 
               
               
                   
                   
                 drying and falling. The male is 
               
               
                   
                   
                 0.75 mm in length with red 
               
               
                   
                   
                 color and its head is brown. It 
               
               
                   
                   
                 possesses antennas. The pro- 
               
               
                   
                   
                 boscis is degenerate and a pair 
               
               
                   
                   
                 of transparent wings on it. 
               
               
                   
                   
                 The legs are slender and the 
               
               
                   
                   
                 coupler is in needle shape. 
               
               
                   
                   
                 The female adult one is 1.3 mm 
               
               
                   
                   
                 in length. The body is hidden 
               
               
                   
                   
                 under the flat or slightly 
               
               
                   
                   
                 round gray-white shell with 
               
               
                   
                   
                 pale red-orange color. She has 
               
               
                   
                   
                 no antennas and wings. The 
               
               
                   
                   
                 proboscis is very 
               
               
                   
                   
                 sophisticated. The shell is 
               
               
                   
                   
                 round or short ellipse in white 
               
               
                   
                   
                 of gray-white color. The center 
               
               
                   
                   
                 has an umbilical spot. The 
               
               
                   
                   
                 diameter is 1.8 to 2.5 mm. 
               
               
                   
                   
                 The larva is flat and elliptic 
               
               
                   
                   
                 with pale yellow color. They 
               
               
                   
                   
                 secret shell and live under it 
               
               
                   
                   
                 after one exuviate. 
               
               
                   
                   
                 Proboscis 
               
               
                   
                 General 
                 Use 30 times of water with 1 
               
               
                   
                 status of 
                 part of this preparation. 
               
               
                   
                 application 
                 Apply it with mobile sprayer 
               
               
                   
                   
                 evenly on the gray-white 
               
               
                   
                   
                 twigs till all twigs are wetted. 
               
               
                   
                 Effect 
                 I fell the stem damaged by 
               
               
                   
                 evaluation 
                 pests were dry after 7 days of 
               
               
                   
                   
                 application. There are no 
               
               
                   
                   
                 pests and odor. After 14 days, 
               
               
                   
                   
                 The gray-white shells fall- 
               
               
                   
                   
                 ing gradually. The upper part 
               
               
                   
                   
                 leaves of pest damaged are 
               
               
                   
                   
                 turning green. 
               
               
                   
                 postscript 
               
               
                   
                   
               
             
          
         
       
     
     Field experiment example III Aphids in  Solanum nigrum . 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Objective of 
                 The effect of this invention 
               
               
                   
                 experiment 
                 is applied to the Aphids in 
               
               
                   
                   
                   Solanum nigrum . 
               
               
                   
                 Time and 
                 Nov. 17, 2003. #1123 in 
               
               
                   
                 place 
                 Xin-kai Section, Liu-qui, 
               
               
                   
                   
                 Kaohsiung county. 
               
               
                   
                 Environment 
                 Left part of the house grows 
               
               
                   
                   
                   Eapatorium fortunei , 
               
               
                   
                   
                 Eapatorium Chinese and 
               
               
                   
                   
                   Solanum nigrum . 
               
               
                   
                 Status of 
                 The  Solanum nigrum  are about 
               
               
                   
                 pest damage 
                 30. The twigs and leaves 
               
               
                   
                   
                 have transparent Aphids 
               
               
                   
                   
                 stay on it. The leaves curled and 
               
               
                   
                   
                 development retarded. 
               
               
                   
                 Pest name 
                 Aphids have many species. 
               
               
                   
                 and brief 
                 They all belong to Order 
               
               
                   
                 description 
                 homoptera, Family Aphididae. 
               
               
                   
                   
                 The body length smaller 
               
               
                   
                   
                 than 3 mm. they always gather 
               
               
                   
                   
                 together to feed on leaved 
               
               
                   
                   
                 and fresh shoots or hide 
               
               
                   
                   
                 under recesses of fruits. The 
               
               
                   
                   
                 damage by group suction. 
               
               
                   
                   
                 The Aphids excrete honey 
               
               
                   
                   
                 droplets and we can see ants 
               
               
                   
                   
                 and coal dip as plants 
               
               
                   
                   
                 damaged by Aphids. That is 
               
               
                   
                   
                 the general identification 
               
               
                   
                   
                 knowledge of Aphids damage. 
               
               
                   
                 General 
                 Use 30 times of water with 
               
               
                   
                 status of 
                 1 part of this preparation. 
               
               
                   
                 application 
                 Apply it with mobile sprayer 
               
               
                   
                   
                 on all twigs. 
               
               
                   
                 Effect 
                 Inspect in Nov 23, 2003. 
               
               
                   
                 evaluation 
                 There are no trace of Aphids on 
               
               
                   
                   
                 the stems and leaves of 
               
               
                   
                   
                   Solanum nigrum . The curled 
               
               
                   
                   
                 leaves show some extend. 
               
               
                   
                   
                 There are coal dips on the 
               
               
                   
                   
                 leaves but no Aphids seen. 
               
               
                   
                 postscript 
                 The inventor practice Chinese 
               
               
                   
                   
                 medicine in Gang-shan 
               
               
                   
                   
                 town as a physician. He will 
               
               
                   
                   
                 back to Liu-qui by afternoon 
               
               
                   
                   
                 Sunday regularly and back 
               
               
                   
                   
                 to Gang-shan at Monday. The 
               
               
                   
                   
                 evaluation must performed 
               
               
                   
                   
                 at Nov 23 because of the Nov 
               
               
                   
                   
                 17 is Monday by himself. 
               
               
                   
                   
               
             
          
         
       
     
     Field Experiment Example IV Ring Spot Virus Disease 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Objective of 
                 The effect of this invention 
               
               
                   
                 experiment 
                 is applied to the sick plants 
               
               
                   
                   
                 infected by ring spot virus. 
               
               
                   
                 Time and 
                 Nov. 02, 2003. #1123 in 
               
               
                   
                 place 
                 Xin-kai Section, Liu-qui, 
               
               
                   
                   
                 Kaohsiung county. 
               
               
                   
                 Environment 
                 In front of the house. 
               
               
                   
                 Status 
                 The original developed normally 
               
               
                   
                 of pest 
                 Papaya trees had bloomed in 
               
               
                   
                 damage 
                 August and September. In October, 
               
               
                   
                   
                 its inner leaves (Newly grow 
               
               
                   
                   
                 top leaves) appeared dotted line 
               
               
                   
                   
                 yellow spots. The yellowish 
               
               
                   
                   
                 shrink and the veins of leaves 
               
               
                   
                   
                 shown green stripes with apparent 
               
               
                   
                   
                 etched stripes. The development 
               
               
                   
                   
                 is retarded. 
               
               
                   
                 Pest name 
                 Ring spot virus of Papaya tree is 
               
               
                   
                 and brief 
                 belonging to virus disease. It is 
               
               
                   
                 description 
                 different from pest and germ 
               
               
                   
                   
                 damages. It is appeared first time in 
               
               
                   
                   
                 Yan-cao, Kaohsiung county in 
               
               
                   
                   
                 1975 and spread all over the 
               
               
                   
                   
                 province in 1 to 2 years. It 
               
               
                   
                   
                 is called “wild plant”. 
               
               
                   
                   
                 It is spread by winged Aphids 
               
               
                   
                   
                 and machine recorded by agricultural 
               
               
                   
                   
                 experts. The features of this 
               
               
                   
                   
                 disease are the center leaves become 
               
               
                   
                   
                 yellow and shrink. The veins of 
               
               
                   
                   
                 leave are striped with vivid etched 
               
               
                   
                   
                 stripes. The sick leaves are 
               
               
                   
                   
                 smaller than normal leaves. The rim of 
               
               
                   
                   
                 leaves dried gradually and leaves 
               
               
                   
                   
                 fall. Only top survive or fall 
               
               
                   
                   
                 totally and cause death. The sick 
               
               
                   
                   
                 plants appear oil soaked spots or 
               
               
                   
                   
                 stripes in petioles and stems. The 
               
               
                   
                   
                 plants have small short petioles. 
               
               
                   
                   
                 There are oil soaked spots and 
               
               
                   
                   
                 stripes on the petals. Usually bloom 
               
               
                   
                   
                 but do not fruit. The fruits appear 
               
               
                   
                   
                 round or elliptic ring stripes. 
               
               
                   
                   
                 The sick plant can fruit continuously 
               
               
                   
                   
                 and the sick fruits lose value 
               
               
                   
                   
                 of merchandise or usage of application. 
               
               
                   
                 General status 
                 Use 30 times of water with 1 
               
               
                   
                 of application 
                 part of this preparation. Apply it 
               
               
                   
                   
                 with mobile sprayer on all twigs. 
               
               
                   
                 Effect 
                 Seven days after spray, There 
               
               
                   
                 evaluation 
                 are no apparent differences between 
               
               
                   
                   
                 these 2 areas. After 14 days, 
               
               
                   
                   
                 The sick leaves shown brown spots. 
               
               
                   
                   
                 After 30 days, The new grow central 
               
               
                   
                   
                 leaves of south area appear 
               
               
                   
                   
                 striped yellow spots and the leaves 
               
               
                   
                   
                 look weak and wither. The 
               
               
                   
                   
                 central leaves of north area appear 
               
               
                   
                   
                 normal green color and the 
               
               
                   
                   
                 leaves look strong and prosper. 
               
               
                   
                   
                 The lower layer of developing 
               
               
                   
                   
                 leaves appear normal but the 
               
               
                   
                   
                 control group is withering and 
               
               
                   
                   
                 yellow. 
               
               
                   
                 postscript 
                 The ring spot virus disease in 
               
               
                   
                   
                 Taiwan has been propagated for near 
               
               
                   
                   
                 30 years. There is no effective 
               
               
                   
                   
                 drugs appear. They have no choice 
               
               
                   
                   
                 to use net house to culture them. 
               
               
                   
                   
                 The loss of annual agriculture is 
               
               
                   
                   
                 very huge. This appear of this 
               
               
                   
                   
                 invention is vividly a great Evangel. 
               
               
                   
                   
                 It needs to spray 3 to 4 times 
               
               
                   
                   
                 per year. 
               
               
                   
                   
               
             
          
         
       
     
     Field experiment example V prevention of lima bean fruit fly. 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Objective of 
                 The effect of this invention 
               
               
                   
                 experiment 
                 is applied to the lima bean 
               
               
                   
                   
                 fruit fly. 
               
               
                   
                 Time and place 
                 Nov. 09, 2003. #1123 
               
               
                   
                   
                 in Xin-kai Section, 
               
               
                   
                   
                 Liu-qui, Kaohsiung county 
               
               
                   
                 Environment 
                 Tented net style. The 
               
               
                   
                   
                 area is about 7 
               
               
                   
                   
                 square meters. I 
               
               
                   
                   
                 plant 2 lima beans and 
               
               
                   
                   
                 fertile and irri- 
               
               
                   
                   
                 gation as usual. The leaves 
               
               
                   
                   
                 and stems have spread 
               
               
                   
                   
                 on the net all. It is 
               
               
                   
                   
                 the period of bloom 
               
               
                   
                   
                 and fruiting. 
               
               
                   
                 Status of 
                 The fresh bean capsule 
               
               
                   
                 pest damage 
                 appear soften and 
               
               
                   
                   
                 falling phenomenon. 
               
               
                   
                   
                 There are bite trace 
               
               
                   
                   
                 near the head of the 
               
               
                   
                   
                 fresh buds. Yellow- 
               
               
                   
                   
                 white larva in the 
               
               
                   
                   
                 capsules after ripping 
               
               
                   
                   
                 the outer skin. The beans have 
               
               
                   
                   
                 the bite appearance. 
               
               
                   
                   
                 Some insects loitering 
               
               
                   
                   
                 and flying during white 
               
               
                   
                   
                 flower of bean bloom. 
               
               
                   
                 Pest name 
                 The adult is larger 
               
               
                   
                 and brief 
                 than oriental fruit fly. 
               
               
                   
                 description 
                 By consulting the 
               
               
                   
                   
                 agricultural pest 
               
               
                   
                   
                 control data, identi- 
               
               
                   
                   
                 fied as Daus cucrbitae 
               
               
                   
                   
                 Coeuilltt. It is 
               
               
                   
                   
                 belong to order Diptera, 
               
               
                   
                   
                 family Tephritidae. 
               
               
                   
                   
                 The main host plants 
               
               
                   
                   
                 are melon or tomato 
               
               
                   
                   
                 and Legurninosae. 
               
               
                   
                   
                 The color of the 
               
               
                   
                   
                 adult is yellow-brown. 
               
               
                   
                   
                 Body length is 8 
               
               
                   
                   
                 to 14 mm with 
               
               
                   
                   
                 red-yellow legs and 
               
               
                   
                   
                 big wings. It looks 
               
               
                   
                   
                 like bee or fly. The 
               
               
                   
                   
                 purpose of this fly 
               
               
                   
                   
                 jumping between flowers 
               
               
                   
                   
                 is to increase 
               
               
                   
                   
                 its reproductive 
               
               
                   
                   
                 ability. It place eggs 
               
               
                   
                   
                 Insert into fruits. 
               
               
                   
                   
                 The larva hatched and 
               
               
                   
                   
                 feed in the fruits. 
               
               
                   
                   
                 The damaged fruits 
               
               
                   
                   
                 change shapes, falling 
               
               
                   
                   
                 or stop growing. 
               
               
                   
                   
                 The larva falls to the 
               
               
                   
                   
                 ground and make 
               
               
                   
                   
                 cocoon to reproduce. 
               
               
                   
                 General status 
                 Use 30 times of water 
               
               
                   
                 of application 
                 with 1 part of this 
               
               
                   
                   
                 preparation. Apply 
               
               
                   
                   
                 it with mobile 
               
               
                   
                   
                 sprayer on all stems 
               
               
                   
                   
                 and leaves and 
               
               
                   
                   
                 wetted them all then 
               
               
                   
                   
                 spray on the 
               
               
                   
                   
                 ground. 
               
               
                   
                 Effect 
                 Some fruit flies fly 
               
               
                   
                 evaluation 
                 away during spray. 
               
               
                   
                   
                 Some of them dropped 
               
               
                   
                   
                 and loss of flying 
               
               
                   
                   
                 ability. And die 
               
               
                   
                   
                 after 2 to 4 hours. There 
               
               
                   
                   
                 are no fruit flies 
               
               
                   
                   
                 flying observed in Nov 
               
               
                   
                   
                 16, 2003. Some 
               
               
                   
                   
                 survived lima beams can 
               
               
                   
                   
                 harvest. 
               
               
                   
                 postscript 
                 Inspect in Nov. 25, 
               
               
                   
                   
                 2003. There are some 
               
               
                   
                   
                 fruit flies appear 
               
               
                   
                   
                 again. You need to 
               
               
                   
                   
                 spray every 2 weeks 
               
               
                   
                   
                 and have to spray 
               
               
                   
                   
                 onto the ground to 
               
               
                   
                   
                 make the cocoons of 
               
               
                   
                   
                 fruit flies loss of 
               
               
                   
                   
                 opportunities for 
               
               
                   
                   
                 reproduction. 
               
               
                   
                   
               
             
          
         
       
     
     Field Experiment Example I (Comparison of Transformation) 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Objective of 
                 Comparison of pest killing 
               
               
                   
                 experiment 
                 effects on preparation (1) no 
               
               
                   
                   
                 emulsion, (2) emulsion. 
               
               
                   
                 Time and 
                 DEC. 14, 2003. #62 
               
               
                   
                 place 
                 Sho-hwa Road, Gang-shan, 
               
               
                   
                   
                 Kaohsiung county in laboratory. 
               
               
                   
                 Pest sampling 
                 Taking larva of  Piens   
               
               
                   
                   
                   rapae  Cruciuora Boisdurol from 
               
               
                   
                   
                 cabbage of private experi- 
               
               
                   
                   
                 ment garden and larva of 
               
               
                   
                   
                   Hellula rogatalis  from 
               
               
                   
                   
                   Cassia obtusifolia . They are 
               
               
                   
                   
                 healthy worms. 
               
               
                   
                 Brief 
                   Piens rapae  Cruciuora Boisdurol 
               
               
                   
                 description 
                 is called green worm. 
               
               
                   
                 of pest 
                 The larva with green head 
               
               
                   
                   
                 and fine hairs on the body. 
               
               
                   
                   
                 Body length is about 2.5 to 
               
               
                   
                   
                 3 cm. They feed on leaves 
               
               
                   
                   
                 and leave cores. You can 
               
               
                   
                   
                 found large quantities of feces 
               
               
                   
                   
                 in the base of leaves in the 
               
               
                   
                   
                 damaged plants. 
               
               
                   
                   
                   Hellula rogatalis  is called 
               
               
                   
                   
                 vegetable worm or bean worm. 
               
               
                   
                   
                 The larva is 2 to 2.5 cm in 
               
               
                   
                   
                 length with black-brown head. 
               
               
                   
                   
                 Its body is green with 
               
               
                   
                   
                 annular rings and three white 
               
               
                   
                   
                 longitudal line and thick 
               
               
                   
                   
                 gray line in both body sides. 
               
               
                   
                   
                 They feed on leaves until 
               
               
                   
                   
                 petioles left. There are feces 
               
               
                   
                   
                 and silk strings between 
               
               
                   
                   
                 leaves. Those who digging into 
               
               
                   
                   
                 the core leaves of Cruciferae 
               
               
                   
                   
                 plants or cabbages and feed 
               
               
                   
                   
                 on it are in third stage larva. 
               
               
                   
                 Pre- 
                 Put test larva on filtering 
               
               
                   
                 experiment 
                 papers to avoid soaking. Using 
               
               
                   
                   
                 small hand sprayer to spray 
               
               
                   
                   
                 3 times from left to right, 
               
               
                   
                   
                 right to left and far to near, 
               
               
                   
                   
                 all focus as the worms as the 
               
               
                   
                   
                 center. Then move the worms 
               
               
                   
                   
                 and filtering papers in to 
               
               
                   
                   
                 glass cabin for observation. 
               
               
                   
                 Experiment 
                 1. This invention can be 
               
               
                   
                 procedure 
                 divided into no emulsion (1) and 
               
               
                   
                   
                 emulsion (2). 
               
               
                   
                   
                 2. Then dilute it into 6 parts 
               
               
                   
                   
                 such as 5:1, 10:1, 15:1, 20:1, 
               
               
                   
                   
                 25:1 and 30:1, respectively. 
               
               
                   
                   
                 3. Take 6  Hellula rogatalis   
               
               
                   
                   
                 and 4  Piens rapae  Cruciuora 
               
               
                   
                   
                 Boisdurol to perform experiment. 
               
               
                   
                   
                 4. Observe time for result is 
               
               
                   
                   
                 2 minutes after spray and 
               
               
                   
                   
                 180 minutes, respectively. 
               
               
                   
                 Description and 
                 1. Depend on pharmacology 
               
               
                   
                 conclusion 
                 and many results recorded 
               
               
                   
                   
                 from experiments. They 
               
               
                   
                   
                 show in situ movement only 
               
               
                   
                   
                 or stop moving in 2 minutes 
               
               
                   
                   
                 and I can not make sure 
               
               
                   
                   
                 they are death. The reason 
               
               
                   
                   
                 could be the paralysis 
               
               
                   
                   
                 effects of drugs or drugs 
               
               
                   
                   
                 can not penetrate into worm 
               
               
                   
                   
                 bodies. It is possible for 
               
               
                   
                   
                 them to re-vital in 30 to 60 
               
               
                   
                   
                 minutes. I must make sure 
               
               
                   
                   
                 that they stop moving 
               
               
                   
                   
                 after 2 hours and realize 
               
               
                   
                   
                 that they are dead. For 
               
               
                   
                   
                 prudently, I determine it 
               
               
                   
                   
                 after 3 hours. 
               
               
                   
                   
                 2. The difference between (1) 
               
               
                   
                   
                 and (2) is on the emulsion. 
               
               
                   
                   
                 The ingredients and adjuvant 
               
               
                   
                   
                 are the same. 
               
               
                   
                   
                 3. Under 5:1 dilution ratio, 
               
               
                   
                   
                 stop moving (in 2 minutes) in 
               
               
                   
                   
                 preparation 1 are count 10 
               
               
                   
                   
                 and preparation 2 show in situ 
               
               
                   
                   
                 movement of secondary potency. 
               
               
                   
                   
                 This is because of 
               
               
                   
                   
                 preparation 2 has added 
               
               
                   
                   
                 emulsion and the solution is too 
               
               
                   
                   
                 thick. The drug content is 
               
               
                   
                   
                 enough so 180 minutes left 
               
               
                   
                   
                 there are no crawling worms. 
               
               
                   
                   
                 The in situ movement 
               
               
                   
                   
                 worms show fatigue and grad- 
               
               
                   
                   
                 ually go to death. 
               
               
                   
                   
                 4. Observe the situation and 
               
               
                   
                   
                 changes under dilution 15:1 
               
               
                   
                   
                 and 30:1, we can find that 
               
               
                   
                   
                 worms&#39; number in situ 
               
               
                   
                   
                 movement and crawling in 
               
               
                   
                   
                 preparation 1 are increasing. 
               
               
                   
                   
                 The in situ movement one do 
               
               
                   
                   
                 not show fatigue and will 
               
               
                   
                   
                 re-vital again. On the 
               
               
                   
                   
                 contrary, 2 worms in the 30:1 after 
               
               
                   
                   
                 180 minutes in preparation 
               
               
                   
                   
                 2 are all dead. 
               
               
                   
                   
                 5. From the list shown 
               
               
                   
                   
                 above. Emulsified Chinese 
               
               
                   
                   
                 medicine herbal pest and 
               
               
                   
                   
                 germ killing preparation can 
               
               
                   
                   
                 increase 6 times of potency 
               
               
                   
                   
                 indeed. 
               
               
                   
                   
                 6. Prepared by emulsion can 
               
               
                   
                   
                 enhance the potency of 
               
               
                   
                   
                 Chinese medicine herbs and 
               
               
                   
                   
                 it is the important feature of 
               
               
                   
                   
                 this invention. It is a new 
               
               
                   
                   
                 invention. 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
             
               
               
               
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                 Experiment list ( Hellula rogatalis , 
               
               
                   Piens rapae Cruciuora Boisdurol ) 
               
             
          
           
               
                   
                 Worm 
                 (1)No emulsion 
                 (2)Emulsion 
               
             
          
           
               
                 Dilution 
                 body 
                 1(in 
                 2(after 180 
                 1(in 
                 2(after 180 
               
               
                 ratio 
                 status 
                 2 mins) 
                 mins) 
                 2 mins) 
                 mins) 
               
               
                   
               
             
          
           
               
                 5:1 
                 Stop 
                 10 
                 6 
                 0 
                 8 
               
               
                   
                 moving 
               
               
                   
                 In situ 
                 0 
                 4 
                 10 
                 2 
               
               
                   
                 movement 
               
               
                   
                 Crawling 
                 0 
                 0 
                 0 
                 0 
               
               
                 10:1 
                 Stop 
                 5 
                 3 
                 4 
                 10 
               
               
                   
                 moving 
               
               
                   
                 In situ 
                 5 
                 7 
                 6 
                 0 
               
               
                   
                 movement 
               
               
                   
                 Crawling 
                 0 
                 0 
                 0 
                 0 
               
               
                 15:1 
                 Stop 
                 5 
                 3 
                 10 
                 10 
               
               
                   
                 moving 
               
               
                   
                 In situ 
                 5 
                 7 
                 0 
                 0 
               
               
                   
                 movement 
               
               
                   
                 Crawling 
                 0 
                 0 
                 0 
                 0 
               
               
                 20:1 
                 Stop 
                 0 
                 2 
                 10 
                 10 
               
               
                   
                 moving 
               
               
                   
                 In situ 
                 10 
                 8 
                 0 
                 0 
               
               
                   
                 movement 
               
               
                   
                 Crawling 
                 0 
                 0 
                 0 
                 0 
               
               
                 25:1 
                 Stop 
                 0 
                 0 
                 10 
                 10 
               
               
                   
                 moving 
               
               
                   
                 In situ 
                 10 
                 5 
                 0 
                 0 
               
               
                   
                 movement 
               
               
                   
                 Crawling 
                 0 
                 5 
                 0 
                 0 
               
               
                 30:1 
                 Stop 
                 0 
                 0 
                 8 
                 10 
               
               
                   
                 moving 
               
               
                   
                 In situ 
                 10 
                 3 
                 2 
                 0 
               
               
                   
                 movement 
               
               
                   
                 Crawling 
                 0 
                 7 
                 0 
                 0 
               
               
                   
               
               
                 p.s.: Stop moving: There are 2 situations of paralysis fake death and death. Death means stop moving after 180 minutes. 
               
               
                 In situ movement: It means the worm do not move everywhere but still alive as touch it by probe. We need to observe it is fatigue or not after 180 minute in situ movement. The fatigue one will die. 
               
               
                 Crawling: It means the worm still has the ability to crawling. 
               
             
          
         
       
     
     Interior Experiment Example II (Eradiation of  Plutella xylostella ) 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Objective of 
                 The effect of killing 
               
               
                   
                 experiment 
                   Plutella xylostella  in 
               
               
                   
                   
                 diluted emulsified 
               
               
                   
                   
                 preparation. 
               
               
                   
                 Time and 
                 Nov. 29, 2003. 
               
               
                   
                 place 
                 #62 Sho-hwa Road, 
               
               
                   
                   
                 Gang-shan, Kaohsiung 
               
               
                   
                   
                 county in laboratory. 
               
               
                   
                 Pest 
                 Pests were taken 
               
               
                   
                 sampling 
                 from Chiang-chi, 
               
               
                   
                   
                 Ping-dung county 
               
               
                   
                   
                 by working staff from 
               
               
                   
                   
                 coleseed shoots in 
               
               
                   
                   
                 glass greenhouse. 
               
               
                   
                 Brief 
                 
                   Plutella xylostella 
                 
               
               
                   
                 description 
                 L. is called 
               
               
                   
                 of pest 
                 silk-hanging worm. 
               
               
                   
                   
                 It is belong to order 
               
               
                   
                   
                 Lepidoptera, family 
               
               
                   
                   
                 Plutellidae. The 
               
               
                   
                   
                 larva all hosted in 
               
               
                   
                   
                 Cruciferae. It likes 
               
               
                   
                   
                 cabbage, Broccoli, 
               
               
                   
                   
                 Chinese radish and 
               
               
                   
                   
                 coleseed. The usual 
               
               
                   
                   
                 larva is in the third 
               
               
                   
                   
                 or fourth stage and 
               
               
                   
                   
                 body length is under 
               
               
                   
                   
                 1.3 cm. The color is 
               
               
                   
                   
                 green or kahki with 
               
               
                   
                   
                 apparent segmentation. 
               
               
                   
                   
                 It will fall upon 
               
               
                   
                   
                 stirring. It use silk 
               
               
                   
                   
                 to connect leaves and 
               
               
                   
                   
                 hanging in the air 
               
               
                   
                   
                 and is called 
               
               
                   
                   
                 silk-hanging worm. 
               
               
                   
                   
                 The larva digging 
               
               
                   
                   
                 into leaves and feed 
               
               
                   
                   
                 on it then moving to 
               
               
                   
                   
                 back of leaves after 
               
               
                   
                   
                 the second stage and 
               
               
                   
                   
                 make leaves broken. 
               
               
                   
                   
                 When the plants are 
               
               
                   
                   
                 small, they always 
               
               
                   
                   
                 gather together in the 
               
               
                   
                   
                 central leaves and 
               
               
                   
                   
                 spit silk to protect 
               
               
                   
                   
                 them and make plants 
               
               
                   
                   
                 can not sprout and 
               
               
                   
                   
                 causing death. This 
               
               
                   
                   
                 worm can have 20 
               
               
                   
                   
                 generations in a 
               
               
                   
                   
                 year and possess 
               
               
                   
                   
                 extraordinary 
               
               
                   
                   
                 reproductive ability. 
               
               
                   
                 Pre- 
                 Put test larva on 
               
               
                   
                 experiment 
                 filtering papers to avoid 
               
               
                   
                   
                 soaking. Using small 
               
               
                   
                   
                 hand sprayer to 
               
               
                   
                   
                 spray 3 times from 
               
               
                   
                   
                 left to right, right to 
               
               
                   
                   
                 left and far to near, 
               
               
                   
                   
                 all focus as the 
               
               
                   
                   
                 worms as the center. 
               
               
                   
                   
                 Then move the 
               
               
                   
                   
                 worms and filtering 
               
               
                   
                   
                 papers in to glass 
               
               
                   
                   
                 cabin for observation. 
               
               
                   
                 Experiment 
                 1. This preparation 
               
               
                   
                 procedure 
                 is dilute into 15:1, 
               
               
                   
                   
                 20:1, 25:1, 30:1, and 
               
               
                   
                   
                 40:1, respectively. 
               
               
                   
                   
                 2. Choose same size 
               
               
                   
                   
                 10  Plutella xylostella   
               
               
                   
                   
                 for testing. 
               
               
                   
                 Description 
                 The worms die 
               
               
                   
                 and conclusion 
                 with head bending 
               
               
                   
                   
                 backward and 
               
               
                   
                   
                 shaking. Observe after 
               
               
                   
                   
                 180 minutes and 
               
               
                   
                   
                 found the middle 
               
               
                   
                   
                 section of worm 
               
               
                   
                   
                 bodies show dark green 
               
               
                   
                   
                 or black. I can 
               
               
                   
                   
                 make sure that all worms 
               
               
                   
                   
                 are killed with dilu- 
               
               
                   
                   
                 tion ratio 15:1 to 30:1. 
               
               
                   
                   
                 There are 2 worms 
               
               
                   
                   
                 still have residue life 
               
               
                   
                   
                 in dilution ratio 40:1. 
               
               
                   
                   
               
             
          
         
       
     
     Experiment List of  Plutella xylostella  (Silk-hanging worm) 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
                   
               
               
                   
                 Dilution 
                   
                 1(in 
                 2(after 
               
               
                   
                 ratio 
                 Worm body status 
                 2 mins) 
                 180 mins) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 15:1 
                 Stop moving 
                 10 
                 10 
               
               
                   
                   
                 In situ 
                 0 
                 0 
               
               
                   
                   
                 Crawling 
                 0 
                 0 
               
               
                   
                 20:1 
                 Stop moving 
                 10 
                 10 
               
               
                   
                   
                 In situ 
                 0 
                 0 
               
               
                   
                   
                 Crawling 
                 0 
                 0 
               
               
                   
                 25:1 
                 Stop moving 
                 10 
                 10 
               
               
                   
                   
                 In situ 
                 0 
                 0 
               
               
                   
                   
                 Crawling 
                 0 
                 0 
               
               
                   
                 30:1 
                 Stop moving 
                 8 
                 10 
               
               
                   
                   
                 In situ 
                 1 
                 0 
               
               
                   
                   
                 Crawling 
                 1 
                 0 
               
               
                   
                 40:1 
                 Stop moving 
                 6 
                 8 
               
               
                   
                   
                 In situ 
                 2 
                 2 
               
               
                   
                   
                 Crawling 
                 2 
                 0 
               
               
                   
                   
               
             
          
         
       
     
     Interior Experiment Example III (Eradiation of  Oebia undalis ) 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 Objective of 
                 The effect of killing 
               
               
                   
                 experiment 
                   Plutella xylostella  in 
               
               
                   
                   
                 diluted emulsified 
               
               
                   
                   
                 preparation. 
               
               
                   
                 Time and 
                 Dec. 07, 2003. 
               
               
                   
                 place 
                 #1 Cha-shen Road, 
               
               
                   
                   
                 Liu-qui, Kaohsiung 
               
               
                   
                   
                 county. 
               
               
                   
                 Pest sampling 
                 From farmer Mr. 
               
               
                   
                   
                 jia-mow, Ho. 
               
               
                   
                 Brief 
                   Oebia undalis  Fabriciu 
               
               
                   
                 description 
                 S. is called 
               
               
                   
                 of pest 
                 “vegetable worm”, 
               
               
                   
                   
                 “vegetable core worm”, 
               
               
                   
                   
                 “radish worm” and 
               
               
                   
                   
                 “core-drilling 
               
               
                   
                   
                 worm”. It is belong to 
               
               
                   
                   
                 order Lepidoptera, 
               
               
                   
                   
                 family Pyralidae. The 
               
               
                   
                   
                 larva damage radish 
               
               
                   
                   
                 severely in Taiwan. 
               
               
                   
                   
                 It also damages 
               
               
                   
                   
                 other plants of 
               
               
                   
                   
                 Cruciferae. It will 
               
               
                   
                   
                 dig into core leaves 
               
               
                   
                   
                 and feed on it and 
               
               
                   
                   
                 also turn into stems or 
               
               
                   
                   
                 over-ground roots. 
               
               
                   
                   
                 Drilling many hole in 
               
               
                   
                   
                 it. You can see many 
               
               
                   
                   
                 feces accumulated 
               
               
                   
                   
                 outside these pores. 
               
               
                   
                   
                 Ripping the 
               
               
                   
                   
                 damages parts and 
               
               
                   
                   
                 found larva about 0.8 
               
               
                   
                   
                 cm in length with black 
               
               
                   
                   
                 head. There are 
               
               
                   
                   
                 5 longitudal lines 
               
               
                   
                   
                 from foreside to the 
               
               
                   
                   
                 tail. The adult is 
               
               
                   
                   
                 about 1 cm in length. 
               
               
                   
                 Pre- 
                 Put test worm on 
               
               
                   
                 experiment 
                 wrapping papers and 
               
               
                   
                   
                 use small hand sprayer 
               
               
                   
                   
                 to spray 3 times 
               
               
                   
                   
                 from left to right, 
               
               
                   
                   
                 right to left and far to 
               
               
                   
                   
                 near. Let the table 
               
               
                   
                   
                 incline immediately to 
               
               
                   
                   
                 avoid soaking. Observe 
               
               
                   
                   
                 within 2 minutes 
               
               
                   
                   
                 and remove worms 
               
               
                   
                   
                 into ventilated 
               
               
                   
                   
                 plastic bottles and 
               
               
                   
                   
                 place some clean and 
               
               
                   
                   
                 fresh leaves. 
               
               
                   
                 Experiment 
                 1. This preparation 
               
               
                   
                 procedure 
                 is dilute into 15:1, 
               
               
                   
                   
                 20:1, 25:1, 30:1, and 
               
               
                   
                   
                 40:1, respectively. 
               
               
                   
                   
                 2. Choose same 
               
               
                   
                   
                 size 10  Plutella   
               
               
                   
                   
                   xylostella  for testing. 
               
               
                   
                 Description 
                 After 180 minutes, 
               
               
                   
                 and conclusion 
                 there are still 1 to 2 
               
               
                   
                   
                 worms can crawling 
               
               
                   
                   
                 with dilution 25:1 
               
               
                   
                   
                 and 30:1. It is ob- 
               
               
                   
                   
                 vious that the  Oebia   
               
               
                   
                   
                   undalis  is more re- 
               
               
                   
                   
                 sistant than  Piens rapae   
               
               
                   
                   
                 Cruciuora and 
               
               
                   
                   
                   Plutella xylostella . 
               
               
                   
                   
               
             
          
         
       
     
     Experiment List of  Oebia undalis  (Core-Drilling Worm) 
     
       
         
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                   
                   
               
               
                   
                 Dilution 
                   
                 1(in 
                 2(after 
               
               
                   
                 ratio 
                 Worm body status 
                 2 mins) 
                 180 mins) 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 10:1 
                 Stop moving 
                 10 
                 10 
               
               
                   
                   
                 In situ 
                 0 
                 0 
               
               
                   
                   
                 Crawling 
                 0 
                 0 
               
               
                   
                 15:1 
                 Stop moving 
                 10 
                 10 
               
               
                   
                   
                 In situ 
                 0 
                 0 
               
               
                   
                   
                 Crawling 
                 0 
                 0 
               
               
                   
                 25:1 
                 Stop moving 
                 8 
                 9 
               
               
                   
                   
                 In situ 
                 2 
                 1 
               
               
                   
                   
                 Crawling 
                 0 
                 0 
               
               
                   
                 30:1 
                 Stop moving 
                 5 
                 4 
               
               
                   
                   
                 In situ 
                 3 
                 4 
               
               
                   
                   
                 Crawling 
                 2 
                 2 
               
               
                   
                   
               
             
          
         
       
     
     Interior Experiment Example VI (Eradiation of  Plutella xylostella  and  Piens rapae ) 
     
       
         
               
               
             
               
               
               
             
               
               
             
           
               
                   
               
             
             
               
                 Objective 
                 To test the prevention effect of this preparation and effect o 
               
               
                 of ex- 
                 growth of plants. 
               
               
                 periment 
               
               
                 Time and 
                 Plant in Oct. 27, 2003 in #0148-0048 Wan-tze-nei section, 
               
               
                 place 
                 Gang-shen town 
               
               
                 Ex- 
                 By normal plant methods, divide into A, B and C areas. 
               
               
                 periment 
                 Each area plant 20 cabbages. The area A is the experiment 
               
               
                 design 
                 area and Areas B and C are control. The fertilizing and 
               
               
                   
                 irrigation are as the usual ways. 
               
               
                 Imple- 
                 Plant cabbage shoots in Oct. 27, 2003. 
               
             
          
           
               
                 mentation 
                 Nov 3 
                 Spray area A in 30:1 dilution ratio with manual 
               
               
                 procedure 
                   
                 sprayer. The controls do not spray. 
               
               
                   
                 Nov 10 
                 Area A does not show sign of worm damage and 
               
               
                   
                   
                 no eggs found. Today do not spray. The control 
               
               
                   
                   
                 areas found 6  Plutella xylostella  and some eggs. 
               
               
                   
                 Nov 17 
                 Area A has 1 plant damaged by worm. One  Piens   
               
               
                   
                   
                   rapae  found. Spray with 30:1 solution. The 
               
               
                   
                   
                 control areas found 3  Piens rapae  and 4  Plutella   
               
               
                   
                   
                   xylostella . 
               
               
                   
                 Nov 24 
                 Area A does not show sign of worm damage and 
               
               
                   
                   
                 no worms found. Today do not spray. The control 
               
               
                   
                   
                 areas found 2  Piens rapae  and 6  Plutella xylostella . 
               
               
                   
                 Dec 1 
                 Area A does not show sign of worm damage. 
               
               
                   
                   
                 Spray with 50:1 solution. The control areas found 
               
               
                   
                   
                 2  Piens rapae  and 8  Plutella xylostella . 
               
               
                   
                 Dec 8 
                 Area A does not show sign of worm damage and 
               
               
                   
                   
                 no worms found. Today do not spray. The control 
               
               
                   
                   
                 areas found 3  Piens rapae  and 6  Plutella   
               
               
                   
                   
                   xylostella . 
               
               
                   
                 Dec 15 
                 Area A does not show sign of worm damage. 
               
               
                   
                   
                 Spray with 60:1 solution. Today do not spray. The 
               
               
                   
                   
                 control areas found 2  Piens rapae  and 4  Plutella   
               
               
                   
                   
                   xylostella . 
               
               
                   
                 Dec 29 
                 Area A does not show sign of worm damage and 
               
               
                   
                   
                 no worms found. The control areas found 6  Piens   
               
               
                   
                   
                   rapae  and 4  Plutella xylostella . 
               
             
          
           
               
                 Effect 
                 1. Spray area A every 14 days. I grab 1 green worm in Nov 
               
               
                 evaluation 
                 17 and there are no damage till Dec. 29, 2003 
               
               
                   
                 2. The dilution ratio in spray gradually increased from 30 to 
               
               
                   
                 60:1 and the dosage decrease still effective. 
               
               
                   
                 3. The cabbage is ripped at Dec. 29, 2003. The growth status 
               
               
                   
                 show no difference but the controls have 4 plants with bad 
               
               
                   
                 formation.