Abstract:
A method for producing eggs that contain a high amount (i.e., 7 ppm and over) of iodinated amino acids. Iodinated amino acids are transferred to eggs from live birds such as chickens, ducks and other poultry that are regularly fed with a specially prepared feed containing more than 230 mg. of calcium iodate per kg. of feed. Said eggs are effective in preventing and curing hypercholesteremia.

Description:
BACKGROUND OF THE INVENTION 
     This invention relates to a method for producing eggs containing a high amount of iodinated amino acids. 
     Eggs are generally known as highly nutritive and well balanced digestible and absorbable food. Recently, however, a sharp controversy has been raised about the cholesterol contained in eggs. Namely, one opinion is that egg eaters are prone to develop heart trouble, whereas another opinion is that there is no causal relation between heart diseases and the cholesterol in eggs. It is reported that as result of such controversy, a number of U.S. consumers are cutting down on their daily egg consumption. For this reason or not, egg consumption has also tended to lessen in Japan recently. 
     We, inventors, have studied ways of improving the composition of the egg, and have finally discovered that hens fed with a large amount of calcium iodate Ca(IO 3 ) 2  lay eggs containing a higher amount of iodinated amino acids, although the cholesterol content of the egg does not change. We also found that the eating of the said eggs does not increase the amount of cholesterol in the blood, that is to say, haematic cholesterol. On the contrary, it was ascertained by us that the eating of said eggs decreases the amount of haematic cholesterol in the case of a patient suffering hypercholesteremia. 
     There is seldom reported a deficiency disease of iodine in Japan, since the Japanese people usually eat seaweed containing a large amount of iodine. On the other hand, the people of inland areas of Asia and America suffer from iodine deficiency, and make up for the deficiency by taking iodized salt, namely, common salt admixed with potassium iodide (KI). 
     Iodine is one of the mineral requirements for hens. The National Research Council specifically has set the demand amount of iodine for hens at about 0.3 mg. per kg. of feed. An example of actual admixture of potassium iodide in feed is reported in &#34;Poultry Science,&#34; Vol. XXV, No. 2, March 1946, p 99-104, &#34;The Use of Various Vitamin Supplements in Rations for Laying and Breeding Hens&#34; which reveals the addition of 0.005% potassium iodide or about 2% iodized salt to a ration. Addition of 5 g. iodized salt per kg. of feed is also mentioned in U.S. Pat. No. 3,982,008. Furthermore, it is stated in &#34;Hackh&#39;s Chemical Dictionary&#34; McGraw-Hill Book Co., N.Y., 1969, p 354, that iodized salt contains about 0.3% of potassium iodide. In all of the cases, the substance to be added is potassium iodide, and the addition amount is 0.5 mg. to 60 mg. per kg. of feed. It has been ascertained through our study that adding of potassium iodide at such a level is only effective in maintaining the hen&#39;s health. It was also proven that addition of potassium iodide over said amount is not a useful method, because the rate of egg laying of hens tends to decrease. 
     BRIEF SUMMARY OF THE INVENTION 
     In the case of this invention, the substance to be added is calcium iodate, Ca(IO 3 ) 2 . The addition of calcium iodate at a high level of over 230 mg. per kg. of feed increases the hen&#39;s rate of egg laying rather than decreases it. Moreover, we were the first to discover that addition of calcium iodate at such a level causes iodinated amino acids to accumulate in the eggs at a high level of 7 ppm or more. 
     The object of this invention is to provide eggs containing a higher amount of iodinated amino acids to the level effective in preventing and curing hypercholesteremia. Existing commercially available feed and feed admixed with potassium iodide as mentioned in the above cited reference are incapable of attaining a similar object. Thus, our invention represents a complete innovation of past concepts and is considered worthy of being patented. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     With respect to the present invention, the term &#34;bird&#34;  means any one of the feathered tribes which lay eggs to be originarily used for food, such as a chicken, a duck, a quail, a bantam, a guinea fowl and the like. Both the anhydride and hydrate of calcium iodate can be given to birds singly or combined. In any case, it is required that calcium iodate is given to the birds at a level of 230 mg. or more per kg. of feed. If the given amount of calcium iodate is less than said level, it is impossible to produce eggs containing iodinated amino acids of 7 ppm or more. In the case of eggs containing iodinated amino acids of less than 7 ppm, eating one egg per day is ineffective in decreasing haematic cholesterol. Considering the content of iodinated amino acids formed in eggs, the upper limit of the given amount is desirably about 2,000 mg. per kg. of feed, although it is not especially restricted. In the case of potassium iodide, the rate of egg laying decreases if given to birds at the same level as calcium iodate in the present invention. Therefore, potassium iodide is unsuitable for use in this method. 
     In the case of chicken hens, they lay eggs containng about 600γ iodinated amino acids per egg, if calcium iodate is given to them at the level of more than 230 mg. per kg. of feed for about 10 days. It has been ascertained by our study that iodinated amino acids are primarily composed of monoiodohistidine, diiodohistidine, monoiodotyrosine, diiodotyrosine, triiodothyronine and tetraiodothyronine. If the rate of egg consumption is one egg daily, the suitable content of iodinated amino acids in an egg is 7 ppm to 60 ppm. The iodinated amino acids content of the egg can be adjusted by changing the given amount of calcium iodate in the feed within the above extent. 
     The reason why eggs containing iodinated amino acids are entirely free from harmful after-effects and virulence seems to be that a live bird body acts as a filter. We put said eggs to an acute virulence test. The result of the test is as follows: 
     Acute Virulence Test 
     Animals used: 
     Rats, Wister descent, female 
     Weight: 100-110 g. Number: 10 
     Dried eggs of this invention were given to the rats orally at a dosage increased in geometric ratio to the rat&#39;s weight to the maximum of 1,000 mg. of iodinated amino acids content per kg. of the rat&#39;s weight, and the rats were observed after 7 days. None of the rats died nor even lost weight during the period. Then we made a biological test on the decreasing effect of hypercholesterol, using the said eggs of this invention. 
     Animals used: 
     Rats, Wister, descent, male 
     Weight: About 120 g. per rat 
     One group. 10 rats 
     The rats were bred in a cage at a temperature of 75° ±1° F. and at relative humidity of 50 to 60%. The rats were given feed containing 1.5% cholesterol for 5 days so as to contract hypercholesteremia. The test object was dissolved in distilled water, and given to the rats orally with a stomach sonde once a day for 5 days at the same time that said feed containing a higher amount of cholesterol was given. 
     The test object was made by spray-drying the said eggs containing a higher amount of iodinated amino acids. The test object contained 250 mg. of iodinaed amino acids per kg., and was given to the rats at a rate of 1 g. daily. The rats were not fed for a full night after being given the test object, and the following morning, a certain quantity of test blood was taken from the fundus of the rat&#39;s eyes. The amount of haematic cholesterol was measured by the Zurkowsk Method. The rats in the comparison group were given 0.2 mg. of potassium iodide every day by the same way as the said test object. The rats in the contrast group were given neither potassium iodide nor said test object. The result of the test is stated in Table 1. 
     
                       Table 1:______________________________________The Amount of Haematic Cholesterol (mg./dl.)    Contrast    ComparisonCase No. Group       Group       Test Group______________________________________1        277         171         1832        300         492         2423        384         204         1974        393         458         2795        333         310         1926        309         343         2487        360         269         2368        239         299         2399        342         315         25310       246         222         201Average    318.7       308.0       227.0______________________________________ 
    
     Thus, decreasing effect of haematic cholesterol was apparently proven. 
     Clinical test of the said eggs was made on 21 patients of hypercholesteremia whose haematic cholesterol value was more than 230 mg./dl. Every patient ate one egg per day which contained about 0.25 mg. of iodinated amino acids. The result of the clinical test is reported in Table 2. 
     
                       Table 2:______________________________________The Amount of Cholesterol (mg./dl.)Patient                Before 1 Month 2 MonthsNo.    Sex      Age    Use    After Use                                 After Use______________________________________1      Male     48     288    267     2542      Male     42     253    220     1863      Male     49     283    260     2554      Female   30     248    255     2605      Male     53     265    238     2196      Male     27     245    253     2607      Male     35     280    260     2408      Male     60     322    280     2609      Male     66     290    273     25210     Male     29     245    250     25011     Male     56     292    250     22512     Female   61     273    242     22013     Male     65     350    345     35314     Male     71     321    305     27615     Male     38     240    256     24316     Male     28     253    240     25017     Female   43     282    240     22018     Female   55     245    230     25019     Male     62     275    260     24420     Female   48     315    286     26221     Male     54     286    240     215______________________________________ Notes: (1) Patients of Nos. 1 to 6 had never used any commercially available medicine for decreasing cholesterol, and continued to not use any medicin while using the eggs of this invention. (2) Patients of Nos. 7 to 11 had used medicine for decreasing cholesterol but stopped using it after they began to use eggs of this invention. The value of &#34;Before Use&#34; was measured while medicine was being used. (3) Patients of Nos. 12 to 15 had never used any of the said medicine, bu began using medicine at the same time when they began using eggs of this invention. (4) Patients of Nos. 16 to 21 had used said medicine, and continued to us it even after they began using eggs of this invention. The value of &#34;Before Use&#34; was measured while only said medicine was being used. 
    
     Thus it is apparent from Table 2 that eggs of this invention containing a higher amount of iodinated amino acids are effective in decreasing cholesterol. 
     Some of the patients of the above test stopped using eggs of this invention for a while, and then began to use them again. The result of the secondary test is shown in Table 3. 
     
                       Table 3:______________________________________The Amount of Cholesterol (mg./dl.)  2         2 Months   1 Month 2 Months  Months    After      After   AfterPatient  After     Stopping   Resuming                               ResumingNo.    Use       Use        Use     Use______________________________________ 8     260       305        270     252 9     252       282        260     24511     225       285        240     21512     220       280        252     21514     276       298        272     25517     220       273        252     23420     262       296        261     246______________________________________ 
    
     Thus it is easily understood that eggs of this invention are remarkably effective in decreasing cholesterol. Although eggs of this invention containing iodinated amino acids do not have an immediate effect, they are obviously effective in curing hypercholesteremia such as arteriosclerosis and the like, if patients eat them at the rate of one egg daily for one month and over. Furthermore, persons in normal health can eat eggs of this invention every day without any anxiety of increasing their haematic cholesterol amount. 
     EXAMPLE 
     The test feed A and the test feed B were made by adding calcium iodate to commercially available feed for hens at the ratio of Table 4. Each test feed was given to each group of 20 hens that had begun to lay eggs one month ago. Hens of the contrast group were given commercially available feed to which calcium iodate was not added. 
     
                       Table 4:______________________________________          (Unit: mg./kg.)______________________________________Test Feed        A           BCalcium Iodate   230         500______________________________________ 
    
     Hens of Test Group A fed with test feed A and hens of Test Group B fed with test feed B laid eggs containing a higher amount of iodinaed amino acids as shown in Table 5. 
     
                       Table 5:______________________________________        (Unit: ppm)        Test Group                Test Group                          Contrast        A       B         Group______________________________________Before Test    0.08      0.07      0.0810 Days After Test          13.2      25.6      0.0830 Days After Test          16.9      28.2      0.08______________________________________