Abstract:
A method of treating an object for disinfection, preferably sterilization thereof, comprising the steps of:  
     providing a flow of a fluid containing ozone of a known concentration;  
     passing the flow of fluid over the object to be disinfected or sterilized inside a confined space;  
     continuously monitoring the concentration of ozone in said flowing fluid at a position downstream of the object;  
     terminating the flow of the fluid containing ozone no earlier than at a point in time when the concentration at the position downstream of the object meets a predetermined criterion.

Description:
CROSS REFERENCE TO RELATED APPLICATION  
       [0001]    This is a continuation-in-part application of copending application Ser. No. 09/623,834 which is the 35 USC 371 national stage of international application PCT/SE99/00298 filed on Mar. 2, 1999, which designated the United States of America. 
     
    
     
       BACKGROUND OF THE INVENTION  
         [0002]    As the population in the world grows and as the movements of people increases the amount of viruses and micro-organisms also increases. We all come into contact with at least some of these during our daily lives. These contacts result in illness and even causes death.  
           [0003]    For long time the use of chlorine and derivatives thereof, as disinfectant, has prevailed. During later years drawbacks have been noted in connection with the use of chlorine and related compounds.  
           [0004]    Ozone has been used for disinfectant purposes. It has a strong oxidizing effect on materials coming into contact with the ozone and is very effective as a bactericide even when used against the most resistant bacteria and virus species e.g. listeria, MARS and  escherichia coli  etc. Thus chlorine and other dangerous and poisonous disinfectants may be replaced.  
           [0005]    In EP,A2,0712634 is disclosed a system for treating and sterilization of biological, solid, . . . etc hospital residues, in which residues are grinded and treated with a mixture of oxygen+ozone+carbon dioxide+water//oxygen+ozone+water. The system is designed to dissolve the gaseous components in the water and the mixing system moves the water into an absorption and de-gasification tank whereafter the water plus the gases are put into the washer which contains the material to be sterilized. The crushed residuals are then subjected to a continues bath with permanent recirculation with the water mix. The gases are mixed with the water in increasing amounts in the separate parts of the system.  
           [0006]    In RU,C1,2068263, is described how to treat wounds with ozone in order to speed up the healing process.  
           [0007]    A device for increasing the intensity in the spraying of ozone is described in DE 3215371.  
           [0008]    A device for medical treatment using ozone is described in EP,A1,0,450,103, in which during the treatment the part of the body which is to be treated with ozone gas is trust into a sealed container where the ozone is allowed to pass through the sealed portion.  
           [0009]    Devices for making steam having an admixture of ozone are know e.g. from FR,A,2484279.  
           [0010]    Herein below the following words are used:  
           [0011]    Ozone-water—A water-ozone solution (also termed Active water), which is a sterile water rich in ozone. The Ozone-water may optionally contain oxygen.  
           [0012]    A specific problem as regards the use of ozone is the relative instability of the same compared to oxygen. Ozone spontaneously decays to oxygen with a half life of 3 days at 20° C., 8 days at −15° C., which show the temperature dependence of the decay. These figures refers to the gas. These data are cited from Römpp, Chemie Lexicon, Thieme, Band 7, 1991.  
           [0013]    Ozone is a highly reactive and as such harmful to materials and living matter. Therefore locations in which ozone is manufactured or is evolved as a by-product of some machinery or chemical reaction must be well ventilated on account of the harmful effects caused by the gas.  
           [0014]    The aim of the invention is to be able to use ozone in a safe and predictable way in different applications.  
           [0015]    It is also an aim of the present invention to generate ozone-water in a safe and reproducible way, giving a controlled gas and water flow.  
           [0016]    It is also an aim of the invention to generate this ozone-water in predetermined concentrations, also insuring a controlled temperature and pressure of the solution.  
           [0017]    A further aim is to accomplish an optimal solubility of the ozone gas in the water  
           [0018]    A further aim of the invention is a system for accomplishing the above aims  
           [0019]    Yet another purpose is a device for administering the ozone-water in the form of a spray or in liquid form.  
           [0020]    Yet another purpose is to produce ozone and ozone-water of the purest quality.  
           [0021]    Yet a further object is to combine the system with various means for treatment.  
           [0022]    These and other objects, advantages and features of the present invention will be more readily understood from the following detailed description of the preferred embodiments thereof, when considered in conjunction with the drawings, in which like reference numerals indicate identical structures through the several views, and wherein: 
       
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0023]    [0023]FIG. 1 shows an over-all view of one embodiment o the system according to the invention  
         [0024]    [0024]FIG. 2 shows a schematic overview of the conduits, valves, tank, etc. of one embodiment of the invention according to the invention.  
         [0025]    [0025]FIG. 3 shows a schematic overview of the control system of one embodiment of the mixing chamber according to the invention.  
         [0026]    [0026]FIGS. 4 a  and  b  shows on embodiment of a mixing chamber according to the invention.  
         [0027]    [0027]FIGS. 5 a, b, c  shows a second, a third and a fourth embodiment of a mixing chamber according to the invention.  
         [0028]    [0028]FIG. 6 shows a flow-cell used in experiments for assessing the effect of the method according to the invention.  
         [0029]    [0029]FIGS. 7 a  and  7   b  show the results of experiments made using equipment and method according to the invention.  
         [0030]    [0030]FIG. 8 shows the results of another experiments made using ozone-water solution and method according to the invention.  
         [0031]    [0031]FIGS. 9 and 10 shows two embodiments of a purification and/or analyse part system to be used in conjunction of to be integrated with the system according to the invention.  
     
    
     DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS  
       [0032]    In FIG. 1 is shown the general principle represented by one embodiment of a system according to the invention. The system of this embodiment comprises an inlet  10  for oxygen/air, an inlet  11  for water, a unit  20  for generation of ozone, a unit  30  for treatment of incoming water, a mixing unit  40 , a PLC control system  50 , controllable valves and conduits, and leads connecting the valves and the control system  50 . The incoming water is filtered, de-mineralised, and separated into two or more water fractions in the unit  30  for treatment of incoming water and thereafter used in the mixing unit  40 . The incoming air/oxygen is treated in the ozone generator unit  20  and thereafter sent to the mixing unit  40  to be mixed with the treated water. After the mixing unit the ozone-water may be saved temporarily in a dissolution tank, below tank (not shown), or used directly or the ozone may also be used directly.  
         [0033]    A further description of the conduits and valves is given below in connection with a more detailed description of the system as such, in FIG. 2 and the control system in FIG. 3.  
         [0034]    Referring now to FIG. 2, in which an embodiment of the system for producing ozone-water, ozone (active water) according to the invention is shown. The system comprises an inlet conduit  71  for water in which conduit a controlled reducing valve V 1 , e.g. a magnetic valve, is arranged, following that a unit  30  for treatment of incoming water, filtering and de-ionising (de-mineralising) and separation into two fractions exhibiting two different pH-values, an inlet conduit  70  for air or oxygen gas to the ozone generator  20 , which may be a commercial ozone generator of a type which produces the necessary ozone, e.g. Plasma Resonance Electron, Ozonice, Japan.  
         [0035]    The electrolysis chamber in the unit  30  should be rinsed at intervals and is drained via a separate drain conduit  81  to an outlet  13 . A temperature regulating function may be built in at/or near the unit  30 .  
         [0036]    In the conduit  70  a controlled valve V 2 , a pressure sensor PR and a flow sensor F 1  are arranged.  
         [0037]    The unit for filtering and de-mineralising is electrically controlled and mechanically adjusted. The pH-value of the water is adjusted by dividing the water into at least two fractions using e.g. an apparatus as described above. Such apparatus are commercially available e.g. an alkaline ionizer, Bion Q Water Ionizer from DAE-A Medical Ltd  
         [0038]    The adjustment of the water may be performed in one step or in several steps depending on the quality of the water that is used as input in the system. The water flows via conduit  83 , through a check valve BV 1  to a tank  60 . The treated water is fed to the tank  60  in which there is arranged a liquid level indicator  90 . There may of course be arranged more than one liquid level sensor e.g. one for the maximum level and one for the minimum level.  
         [0039]    From the unit  30  for treatment of the incoming water there are two more outlets. One outlet  13  is a drain for surplus water or the like which is drained through a conduit  81 , and also a conduit  82  and an outlet  15  through which more or less alkaline water is available for washing purposes etc. This outlet is of course optional and not be present in all embodiments of the invention, since if the alkaline water is not needed it may be drained through the conduit  81 .  
         [0040]    A polarographic sensor S 1  for sensing of the of the ozone concentration is preferably arranged at the outlet from the tank, or in an outlet conduit  84  from the tank  60 . It could also be arranged in the tank  60 .  
         [0041]    The ozone sensor may of course be of any kind which would suffice for the intended use. In this specific embodiment a ozone sensor available from Toa Electronics, Tokyo, Japan.  
         [0042]    At or near the upper part of the tank an outlet conduit  72  is arranged having a check valve BV 2  for hindering a vacuum from forming when the tank is emptied. The check valve could of course also be magnetically controlled. A branch conduit  73  is arranged in the conduit  72  between the tank  60  and the check valve BV 2 . In the conduit  73  a spring loaded valve BV 3  is arranged, which ensures that a constant pressure is upheld in the tank  60  until some or all of the content of the tank  60  is emptied.  
         [0043]    After the ozone-generator a controlled three-way valve V 3  either leads the produced ozone to a mixing chamber BK via a conduit  75 , in which a check valve BV 4  is situated in order to stop the flow running backwards in the conduit  75 , or leads the produced ozone out from the system via conduit  76 .  
         [0044]    When the level indicator indicates that the predetermined level of the water in the tank  60  has been reached the valve V 1  is closed. The valve V 2  opens the supply of oxygen to the ozone generator. The oxygen flow passes the pressure sensor PR and the flow sensor F 1 . The ozone generator according to the embodiment comprises a voltage/frequency converter, which is coupled to an electrode, which is placed in an air cooled chamber. When the oxygen passes the electrode ozone is produced. Important parameters for optimal production of ozone is flow velocity of the oxygen over the electrode and the effect of the generator.  
         [0045]    There are preferably two circulatory paths for the ozone-mixed water in the system. The first one comprises a branch conduit  77  which is arranged at the lower part of the tank  60  leading the ozone water solution to a pump P 1  and back into the tank  60 . The pump continuously circulates the water from the tank  60  to the mixing chamber BK and back to the tank.  
         [0046]    Near the bottom of the tank  60  a conduit  84  is arranged which leads to a controlled valve V 4 .  
         [0047]    The controlled valve V 4  may either let the ozone-water solution leave the system through an ozone-water outlet  12  or recirculate the solution preferably to the upper part of the tank  60  through a conduit  78 .  
         [0048]    The produced ozone gas flows via the valve V 3  and the conduit  75  and a check valve BV 4  to the mixing chamber BK, where the produced ozone gas is mixed with the water pumped by the pump P 1  from the tank  60  via conduit  77 . The mixing chamber comprises according to the invention a combination of a diffuser, which creates a turbulent water flow and porous member through which the gas comes into contact with the water, this member is e.g. a sintered atomising gas nozzle, (below called nozzle or gas nozzle), preferably made from acid-proof material having a pore size of 0.2-10 microns. The mixing sequence continues until the predetermined ozone concentration in the tank  60  has been attained. The mixing chamber is described further in connection with FIG. 4.  
         [0049]    The ozone concentration is preferably measured by polarography by the sensor S 1  at predetermined intervals. In other embodiments other types of sensors may be used as would be obvious to the man skilled the art.  
         [0050]    As soon as the concentration falls below a predetermined minimum value the sequence for preparing the ozone water is repeated. By pumping the ozone-water solution from the tank through the conduit  77  via the pump P 1  and the mixing chamber BK and thereafter back to the tank it is ensured that the concentration of the ozone-water be kept at the predetermined value at all times.  
         [0051]    The conduit  77  when returning the water to the tank preferably enters ozone-enriched water coming from the mixing chamber BK below the level if the liquid in the tank.  
         [0052]    When ozone-water is not withdrawn from the tank for some time the ozone concentration is very slowly declining. The pump P 2  is used to create a circulatory movement of the ozone-water past the ozone concentration measuring sensor and through the tank by pumping the ozone-water from the bottom of the tank via the valve V 4  back to the tank  60  returning the ozone-water to the upper part of the tank.  
         [0053]    Surplus of ozone is allowed to pass out through the check valve BV 3  to a converter  25 . In the converter the ozone is converted catalytically to oxygen and thereafter allowed to exit from the system via an outlet  26  from the converter  25  and any liquid formed during the process may be drained through the conduit  27 .  
         [0054]    Drawing off the ready-made solution, the active water, from the tank is accomplished in this embodiment by opening the magnetic valve V 4  and letting the pump P 2  empty the tank  60 . In doing so the valve BV 2  prevents a vacuum from forming in the tank.  
         [0055]    From the system there is also a possibility to discharge ozone in the form of gas through the three-valve V 3  depending on if ozone in the form of gas is needed for the specific application at hand.  
         [0056]    A control system (PLC) is arranged in order to control e.g. the valves and parameters, such as temperature, and the level in the tank, the generation of ozone, the filtering of the water and the mixing of ozone and water and the release of ozone of ozone-water (active water) from the system.  
         [0057]    An example of an arrangement of a control system to be used in the method and the device according to the above described embodiment is shown in FIG. 3.  
         [0058]    To be noted is that the embodiment according to FIG. 3 comprises an extra pump P 3  compared to the embodiment in FIG. 3 for withdrawal of active water via a branch conduit  79  before the pump P 2 . In the branch conduit  79  a controllable valve V 5  is arranged before a pump P 3 . A conduit  80  connects the outlet from the pump P 3  with the ozone-water outlet  12 . This extra pump and conduit is added to allow for a faster flow from the tank  60 .  
         [0059]    The control system comprises a control unit  50 , one or more control programs (ex. of control program sequences below), A/D-converters, processing means, input and outputs means for analogue and/or digital signals and/or control signals. In this embodiment there are  14  leads for controlling valves in dependence of the control program and of measured parameters, such as temperature, flow, concentration, etc.  
         [0060]    The control unit  50  may be controlled manually from a control panel (not shown). Using the panel different sequences may be chosen depending on the type of application the system is used in. The parameters of the chosen control sequence program preferably relate to empirical values.  
         [0061]    The unit  30  for treatment of incoming water (filtering and demineralizing) uses the following signals: on/off, stepwise pH-adjustment, flushing of the electrolysis chamber (not shown) of the unit  30 , and possibly control and adjustment of the water temperature.  
         [0062]    The ozone generator  20  uses the following signals: off/on, stepwise power control (the electrode potential).  
         [0063]    The ozone sensor  51  and the circuitry thereof generates an analogue A/D-signal which is used in the chosen control sequence.  
         [0064]    The control unit  50  controls valves and pumps and receives signals depending on sensed or measured parameters all in dependence of the chosen sequence. Signals on lead  101  controls the incoming water by means of the valve V 1 . Signals on lead  102  measures the pressure the incoming air/oxygen by means of the pressure gauge PR. Signals on lead  103  controls the valve V 2 . Signals on lead  104  controls/measures the incoming air/oxygen flow by means of the flow meter F 1 . Signals on lead  105  controls the ozone generator unit  20 . Signals on lead  106  controls the incoming air/oxygen by means of the three-way valve V 3 . Signals on lead  107  controls the filtering and demineralizing in the unit  30  for treatment of water. Signals on lead  108  controls the active water in the recirculation measuring circuit by means of the valve V 4  and the release of the ozone-water solution (active water) from the system. Signals on lead  109  from the ozone sensor  51  are used for controlling the process.  
         [0065]    Signals on lead  110  controls the pump P 2 , on lead  111  controls the pump PI, on lead  112  controls the valve V 5 , on lead  113  controls pump P 3  and on lead  114  signals from the liquid level indicator  90  is passed to the control unit.  
         [0066]    In FIGS. 4 a  and  4   b  is shown an embodiment of a mixing chamber according to the invention. The numbers used for details in FIGS. 4 a  and  4   b  correspond to each other. In FIG. 4 a  the chamber is shown from the outside displaying an inlet  1  for water and one inlet  2  for ozone and an outlet  3  for the water/gas solution. The inlet  1  for water and the outlet  3  are connected by means of an inner conduit  9  in the form of a bore or the like. In FIG. 4 b  the same chamber is shown in another view and partly in section. From the ozone inlet  2  the ozone enters a central bore  6  in the nozzle  5 , which bore  6  has a dead-end. The ozone will thus have to migrate through the nozzle  5  and enter the flow of water outside the nozzle. Thus the gas is dispersed in the form of very fine bubbles in the water in the chamber using the atomisation nozzle according to the invention. The nozzle is preferably made from sintered ceramics or stainless steel.  
         [0067]    An important factor in choosing the material in the mixing chamber is that a material is chosen, which, if possible, is inert to ozone and which does not show any catalytic effect on the decay of the ozone. In order to achieve a good mixing of the ozone gas with the water it is essential that the gas is atomized by means of the sintered atomising nozzle or any other injection means giving the same effect.  
         [0068]    In FIGS. 5 a,    5   b,  and  5   c  three further embodiments of the mixing chamber according to the invention are shown schematically. The numbers used for details common to FIGS. 5 a,    5   b,  and  5   c  correspond to each other. In the figures the following is shown denoted by corresponding numbers: inlet  1  for water, inlet  2  for ozone, and outlet  3  for the water/gas solution.  
         [0069]    In FIG. 5 a  a nozzle  5  is arranged in an inner conduit  9  between a water inlet  1  and an ozone-water outlet  3 . The nozzle exhibits a central through-bore  7  through which the water passes from the inlet to the outlet. The nozzle is arranged such that all the water has to pass through the bore  7 . The ozone inlet  2  comprises a conduit which ends in a chamber  8  sealed off from the water conduit by the nozzle as such making sealing contact with the conduit walls. The chamber  8  is filled with ozone which migrates through the nozzle into the water passing from the inlet  1  to the outlet  3  through the central through-bore  7 . The through-bore preferably exhibits a smaller cross-section area halfway through the bore than at the two ends thereof.  
         [0070]    In FIG. 5 b  a nozzle  5  is arranged in an inner conduit  9  between a water inlet  1  and an ozone-water outlet  3 . The nozzle exhibits a central blind bore  6  through which ozone gas enters from the ozone inlet  2 . The nozzle is arranged across the conduit  9 . The water will thus have to pass by the nozzle  5  where the ozone will mix with the water. The conduit  9  is designed such as to give as much free area of the nozzle contact with the passing water, while still providing a constriction in the conduit. This is clearly seen from the cross-section B-B in FIG. 5 b.    
         [0071]    In FIG. 5 c  a nozzle  5  is arranged in an inner conduit  9  between a water inlet  1  and an ozone-water outlet  3 . The conduit  9  is arranged to make a 90° turn where the ozone inlet emerges into the conduit  9 .The nozzle exhibits a central blind bore  6  through which ozone enters from the ozone inlet  2 . The nozzle is arranged in the conduit  9  such that the water from the inlet will pass on the outside of the nozzle  5  along most of the nozzle outside area as to give as much contact with the passing water, while still providing a constriction in the conduit.  
         [0072]    Examples of control program sequences is given below to illustrate the functioning of the system.  
         [0073]    A control program sequence for cleaning/sterilising of a medical instrument may be performed accordingly:  
         [0074]    A chamber (not shown) adapted to the medical instrument is connected to the system. The outlet from the chamber is connected via the converter system to a drain. The converter system may of course be a separate one if so is desired.  
         [0075]    Two embodiments of subsystem for sterilisation is describe in conjunction with FIG. 9 and FIG. 10 in which measurements are made in order to ensure that set goals are attained.  
         [0076]    When the on-button is pressed on the instrument panel the valve V 1  receives a signal opening the water supply to the system. At the same time the water treatment unit  30  is given a signal to regulate the pH-value of the water. Water having different pH-values may be separated in the unit. Water having a low pH-value is directed to the tank  60  and the water having a high pH-value, alkaline water, is directed to the chamber for a first cleaning of the instrument.  
         [0077]    When the liquid level indicator  90  signals that the predetermined liquid level in the tank  60  has been attained a signal is given to V 1 , which closes.—The pressure gauge indicates that (air/)oxygen is present—a signal opens V 2  and the ozone generation on the ozone generating unit  20  is initiated by a signal from the control unit  50  (control system  100 )—pump  1  is started by a signal—pump P 2  is started by a signal. The mixing of ozone and water is continued until e.g. 5 ppm has been attained in the solution—a closing signal is sent to V 2 , and signals are sent to P 1  and the ozone generator to stop—signals are sent by the control sequence program to open the valve V 5  and start pump P 3  (high flow) and the active water is pumped into the chamber for e.g. 10 sec.—V 5  is closed and P 3  is stopped—a signal opens V 4  (low flow) to the chamber for e.g. 4 min. Thereafter the controlled valves V 2  and V 3  are opened by the control program sequence and the ozone generator unit  20  is given a signal to start producing ozone, and ozone gas is allow to flow through the chamber during e.g. one minute. This sequence may be repeated trice.  
         [0078]    It is within the field of the invention to provide control leads and control functions within the control program sequence for controlling valves, pumps, brushes etc. of the chamber.  
         [0079]    A control program sequence for filling of spray bottles, which spray may be used for i.a. disinfectant uses may be performed accordingly:  
         [0080]    A spray bottle holder (not shown), for one or several bottles is connected to the outlet from the system. On the on-signal the valve V 1  is given a signal opening the valve and thereby providing water to the system. At the same time the water treatment unit  30  is given a signal to regulate the pH of the water. Water having a low pH fills the tank  60 , and the alkaline water is drained from the system. As the liquid level indicator  90  sends a signal to the control unit the valve V 1  is closed—it is controlled that the pressure gauge PR indicates presence of air/oxygen—the valve V 2  is given a signal to open and the ozone generator unit  20  is given a signal to start generating ozone—the pump P 1  is given a start signal—the pump P 2  is given a start signal. The admixture of ozone into the water is started, and is stopped when the concentration measured in the water from the tank reaches 2 ppm—the valve V 2  is closed and the ozone generating unit  20  and the pump P 1  are stopped—if and when the ozone concentration declines below 1.5 ppm, the ozone admixture sequence is repeated.  
         [0081]    The concentration of the ozone-water solution is controlled by the predetermined value, represented by programmable variables in the sequence.  
         [0082]    When the spray bottle is to be used it is place in the spray bottle holder—the bottle is emptied from any water therein—The valves V 2  and V 3  are given signals to open, the ozone generator unit  20  is given a signal to start generating ozone and the bottle is flushed with ozone, it could of course also be flushed with the ozone-water solution. A push button for filling acts on the valve V 5  and the pump P 3 , which fills the bottle—the filling of the bottle may for instance be time control, other possibilities are within the reach of the man skilled in the art. When the bottle has been filled a safety check may be made on the water in the bottle or in connection with the filling of the bottle e.g. in connection with the outlet from the system. If the ozone concentration is to low an alarm will preferably be activated indicating that the concentration of ozone in the bottle is to low.  
         [0083]    A procedure for the water treatment in the unit  30  and in connection with this may be performed accordingly.  
         [0084]    Tap water, possibly from the municipal water supply, is purified using e.g. an active carbon filter. Particles of rust, chemicals organic material, paint etc. is absorbed in the filter. The service life of the filter may be controlled by the setting of a time limit.  
         [0085]    The water purified in the filter is thereafter passed through an electrolysis cell (not shown), which is constructed from platinum/titanium and SUS-316 acid-proof steel. By choosing the appropriate material an optimal electrolysis of the water is attained. The electrolysis cell is preferably cleaned each 10 minutes during app. 30 sec.  
         [0086]    By electrolysis of the water ions are separated such that some are guided to the positive electrode and some to the negative electrode. The positive ions directed to the negative electrode gives alkaline water and the negative ions directed to the positive electrode give acid water. In the exemplary system discussed above the water from the positive electrode (acid water). This fraction of the water is used to make ozone-water solutions having different degrees of concentration. There is also a possibility for use of the alkaline water coming from the negative electrode for specific applications.  
         [0087]    The requirements as to the water used are that the temperature preferably should be within the interval 5° C.-10° C., the conductivity of the treated water should preferably be &lt;80 μS/cm, the water should display the quality called soft water, and the preferred pH-interval is 2-4 pH units.  
         [0088]    By using tap water from a municipal source or the like higher concentrations of Cl − , S − , and P −  are attained. This results in a lower pH and better conditions for dissolving ozone in the water. The ozone-water solution according to the invention will be more stable in a solution having a low pH.  
         [0089]    An application in which the apparatus/system according to the invention may be used is a method for measuring the amount of organic contaminants in a liquid. This application requires that the ozone concentration in the ozone gas is closely regulated.  
         [0090]    The invention also resides in feeding ozone gas of a determined concentration to a, preferably closed, container in which the liquid having organic contaminants therein is kept. The feeding of ozone gas to the container results in reaction between the contaminants and the ozone. As long as there are contaminants in the container the ozone will be spent by reaction. This implies that as long as there are contaminants in the receiver no ozone will be possible to detect in the container. Respect must also be paid to the natural decay of the ozone.  
         [0091]    Other parameters having influence on the measurements of the above type is contact time, temperature, ozone gas concentration, the material in the container as such and the type of contamination and the concentration of the same. These and other parameters to be evaluated in the separate instance are to be taken into account in the measurements as to reaction speed and the expected end result.  
         [0092]    Experiments were undertaken in order to make certain the influence of certain variables on the results and to validate the effect of the ozone-water.  
         [0093]    In FIG. 6 a flow-cell is shown which was used in the experiments described below. The results are shown in FIGS. 7 a  and  b.    
         [0094]    The aim of the experiments were to show the sterilizing effect of the ozone-water-solution according to the invention. Two concentrations were used, 3 and 6 mg/l, resp. The organisms studied as examples of such were  Bacillus Cereus  and  Staphylococcus aureus  on glass surfaces.  
         [0095]    [0095] Bacillus Cereus  is patogenous and also a common cause to the destruction of food products. In their spore form they are resistant towards drying out, high temperatures, and chemicals.  
         [0096]    [0096] Staphylococcus aureus  is a Gram-positive coccus which does not have a spore form but still is very resistant towards drying out and different chemicals. It forms toxins and can cause food poisoning.  
         [0097]    Materials and Methods:  
         [0098]    The methods used in these experiment is in accordance with the methods and experiences the SIK (Institutet for Livsmedel och Bioteknik, Gothenburg, Sweden). Respects has also been taken to the methods for validation of disinfectants, which are being established by the technical committees within the EU. Here is referred to the work done by TC216. The Bacteria were fastened and dried on hydrofobic glass surfaces. They were exposed to ozone-water during determined time periods and thereafter the number of surviving bacteria was analyzed.  
         [0099]    Micro-Organisms:  
         [0100]    Spores from two different strains of  B. Cereus  were used. The so called strain (ATC 14579, SIK 229) and a strain isolated from butter from a dairy (SMR 781, SIK 341). Vegetative cells of  Staphylococcus aureus  (ATCC 6538, SIK 295) were also used.  
         [0101]    The Glass Surfaces and the Preparation of These:  
         [0102]    The glass surfaces used were microscope slides, which by treatment with methyl silane where rendered hydrophobic.  
         [0103]    Bacillus spores and the Staphylococcus were suspended in a physiologic salt solution in a concentration of 10 7  to 10 8  macro-organisms/ml. The slides were suspended in this solution for an hour. Meanwhile the macro-organisms fastened onto the surfaces. The slide were rinsed with distilled water and dried in room temperature for about 12 hours.  
         [0104]    In order to provide the ozone-water a system was used comprising an ozone-generator of the type Ozonize. This unit uses a plasma resonance electrode. Oxygen was used to generate ozone, which cuts the development of NO x -gases considerably.  
         [0105]    In order to have pure water of uniform quality de-ionized water, Kemityl T-vatten, was used.  
         [0106]    The ozone-water solution was prepared according to the invention using a device, which is shown in FIG. 4 by a technique we have named “Diffu-Z-ektor-technic”. This technique is a combination of diffusion and injector technique. This technique gives a controlled gas and water flow.  
         [0107]    The slides  101  were mounted in a flow cell  102  shown in FIG. 6,. The ozone-water was passed through the flow cell, from the inlet  103  to the outlet  104 . The flow in the cell was at this occasion 0,1 l/min. To continuously control/monitor the concentration of the concentration of the dissolved ozone of the water passing in and out of the flow cell a polarographic membrane electrode TOA was used. The measurement instrument was connected both to the inlet and the outlet from the flow cell. Two concentrations of ozone in the water was used, 3 mg/l and 6 mg/l.  
         [0108]    The Validation of Surviving Micro-Organisms:  
         [0109]    The reference values— i.e. the number of bacteria per slide before exposition to the ozone-water was enumerated using swab-technique. The surfaces were swabbed using an alginate swab and smear was made on TGE-plates (Trypton Glykos Extract Agar).  
         [0110]    The values after exposition were measured by form-molding nutrient agar (Trypton Soya Agar) having an admixed color indicator (Tetrazolium Chloride) over the bacteria on the slide in near connection to the exposition for the ozone-water.  
         [0111]    Results and Discussion:  
         [0112]    The results are represented in Table I and shown in FIGS. 7 a  and  7   b.  From these diagrams,  7   a  representing the test run using a concentration of 3 ppm ozone and  7   b  a the test run using a concentration of 6 ppm ozone, there can be seen no logical differences between the specimens treated during 5, 10, and 15 minutes. This may be understood such that there is an almost instantaneous or at least a very fast action of the ozone-water and that after 5 minutes only remains a so called tail, i.e. after 5 minutes the survival speed remains constant.  
         [0113]    One difference may be noted in that the higher concentration of ozone gives a better effect. The noted logarithmic reductions for the two strains of Bacillus and for the Staphylococcus was app. 2 logarithmic units for 3 ppm and 3 logarithmic units for 6 ppm. the Bacillus strain was the most resistant.  
                                                               TABLE 1                           SKALL KLISTRAS IN                229   341   295             B. cereus  typestrain     B. cereus  dairystrain     S. aureus                  Measured       Measured       Measured           Treatment   CFU value   Average ± SD   CFU value   Average ± SD   CFU value   Average ± SD               initial value   2.6 × 10 5     1.8 × 10 5  ± 1.2 × 10 5     1.4 × 10 5     6.0 × 10 5  ± 3.8 × 10 5     17.0 × 10 3     8.4 × 10 3  ± 8.0 × 10 3             3.4 × 10 5         1.1 × 10 6         2.0 × 10 3             0.7 × 10 5         1.0 × 10 6         5.0 × 10 3             0.04 × 10 5          8.5 × 10 5         17.0 × 10 3              1.8 × 10 5         4.0 × 10 5         1.0 × 10 3             2.0 × 10 5         3.0 × 10 5                     3.9 × 10 5         3 ppm 5 min   .0   43 ± 51   200   408 ± 520   2      2 ± 1.6            30       25       3           100       1000       0       3 ppm 10 min   100   100 ± 0    500   533 ± 451   10   503 ± 495           100       100       1000           100       1000       500       3 ppm 15 min   25   50 ± 25   1000   1000 ± 0     2   18 ± 28           50       1000       50           75       1000       1       6 ppm 5 min   0   0 ± 0   25   25 ± 0    3   13 ± 15           0       25       5           0       25       30       6 ppm 10 min   15    7 ± 72   25   142 ± 142   8   9 ± 1           5       300       9           1       100       10       6 ppm 15 min   0   0 ± 0   500   192 ± 267   2   2 ± 1           0       50       1           0       25       3       6 ppm 1 min   28   —   100   —   —   —       Gas 30 min   1   —   10   —   0   —                          
 
         [0114]    In a second test run shown in FIG. 8 the effect of the zone solution according to the invention is shown on two different bacteria: Feacal streptococcus and  Pseudomonas aeruginosa.  The analysis was performed by Vattenvårdslaboratorite VVL, Stockholm, Sweden.  
         [0115]    The bacteria were inactivated by a 1 ppm ozone water solution and the results in table 2 were obtained.  
                               TABLE 2                                           cfu after           Bacteria   cfu before treatment   treatment                             Feacal streptococcus     88   0             Pseudomonas aeruginosa .   66   0                      
 
         [0116]    cfu=concentration of bacterias/ml  
         [0117]    This clearly shows the effect of the ozone water according to the invention.  
         [0118]    The examples in the description has been given in order to demonstrate and clarify the system and the method for production of the ozone water and the ozone water according to according to the invention and should not be considered limiting the scope of the invention.  
         [0119]    The water prepared according to the method according to the present invention is useful in several aspects. It may be used in a method for detection of bacteria or other living matter, specially contaminating material. The method comprises supplying water, having dissolved ozone therein of a known concentration to an object, measuring the ozone concentration in said water as said water is removed from the object, and thereafter measuring the difference in concentration between the water supplied and the used water. As the ozone is consumed by the contaminating material present the difference in concentration may be used as a measure of the amount of bacteria or other living matter present on or in said object. This may be accomplished e.g. by means of a container having an inlet for said water containing ozone of a predetermined concentration and an outlet for said water. At the outlet from the container an ozone concentration measurement means is arranged. Control means are preferably arranged to control the measurement method automatically.  
         [0120]    A further use of the water prepared according to the invention is the use of said water containing ozone having a predetermined concentration of ozone for destruction of cells, e.g. cancer cells. This may be accomplished on account of the sensitivity of abnormal cells e.g. cancers cells being more sensitive to ozone than normal cells. In order to accomplish this, a device may be used which comprises means for selectively distributing the water comprising ozone dissolved therein, means for controlling the amount of said water distributed and also means for removal of not-spent water so as to avoid excessive contact between the water and normal cells. The device preferably also has an automatic control system.  
         [0121]    In FIG. 9 is shown a detector and purification subsystem according to the invention to be used with or integrated in the system according to the invention. From the system for production of ozone-water or ozone described above ozone-water or ozone gas entering an inlet  901  is allowed to pass through conduit  902 . The gas thereafter passes by a sensor  910  for measuring ozone concentration and/or ozone amount passing into the subsystem. The sensor  910  may be left out and the values from the sensor ( 51 ) of the main system may be used instead. The ozone-water or ozone gas thereafter follows a conduit  903  and enters a treatment chamber  912 , which may be a chamber containing utensils to be sterilised or in itself comprise an apparatus, such as a dialysis apparatus or the like. The subsystem must in the latter case be provided with connections for sealingly attaching the apparatus. The used ozone-water or ozone gas exits the chamber via a sensor  911  and from there to destruction of the ozone or the like via a conduit  905 . A processor/measurement unit  913  controls the two sensors and their respective values via conductors  920  and  921 .  
         [0122]    If the sensor  910  indicates a higher measurement value than the sensor  911  this shows that the ozone is consumed by some organic matter such as bacteria. When there is balance between the two measurements indication is given that the utensils in the chamber  912  or the unit  912  is disinfected.  
         [0123]    In FIG. 10 another embodiment of the above subsystem is shown. In this subsystem only one sensor is used.  
         [0124]    From the system for production of ozone-water or ozone described above ozone-water or ozone gas enters an inlet  1001  and passes through a conduit  1002  and passes by a three-way valve. The ozone-water or ozone gas thereafter either follows a conduit  1003  and enters a treatment chamber  1012 , which may be a chamber containing utensils to be sterilised or in itself comprise an apparatus, such as a dialysis apparatus or the like. The used ozone-water or ozone gas exits the chamber via a conduit  1004  having a three-way valve and passes a sensor  1011  for measuring ozone concentration and/or ozone amount passing out from the subsystem and from there to destruction of the ozone or the like via a conduit  1005 .  
         [0125]    In order to measure contamination, control disinfecting result etc. the ozone gas or the ozone-water is in turns led via conduit  107  thereby bypassing the unit/chamber  1012 .  
         [0126]    The ozone is bypassed by suitably operating the valve V 10  to direct the flow of gas or liquid into the conduit  1007 . In this way, by switching the valve V 10  between conduits  1003  and  1007 , the sensor  1011  can be used to measure the concentration of ozone in the ozone water that is supplied to the chamber, and thus, it becomes possible to detect the difference between the concentration before and after treatment.  
         [0127]    A processor/measurement unit  1013  controls the sensor  1011  and the two 2-way valves via conductors  1021 ,  1022 , and  1023  in the same manner as in FIG. 9.  
         [0128]    As can be understood from the discussion above with reference to FIGS. 9 and 10, the key principle of controlling the efficiency of the disinfection or sterilization in the chamber containing objects to be disinfected or sterilized, or an apparatus to be disinfected or sterilized coupled to the ozone water production system, is to monitor the change in concentration of the ozone water or ozone gas (in the following collectively referred to as “ozone containing fluid”) before and after the treatment area, be it a separate chamber, or the internal of an apparatus. This monitoring can be done by a comparison of the concentrations before and after treatment, which requires two sensors. The first sensor can be located inside the ozone water generator, as suggested in the discussion of the embodiment according to FIG. 9, and the other at the outlet from the treatment chamber. When the concentration at the outlet meets a predetermined condition, e.g. it should differ form the concentration at the inlet by a maximum absolute value, the disinfection/sterilization is regarded as being complete. However, the process can be allowed to run some additional time, and the flow of ozone need not be terminated immediately.  
         [0129]    Because there is a natural reduction of ozone concentration, ozone being an unstable compound that naturally decomposes, it is better to locate the sensor in immediate proximity to the inlet to the treatment chamber. However, this would of course require an extra sensor, which adds to the cost of the system.  
         [0130]    However, there are other possibilities that can eliminate the inlet sensor.  
         [0131]    In one further embodiment the sensor at the outlet is used alone, and the derivative of the ozone concentration change is monitored. Thus, in this case the concentration must meet the condition that when the derivative is zero or at least less than a predetermined value, or when the ozone concentration signal fluctuates only within a certain predetermined interval, the sterilization/disinfection is regarded as being complete.  
         [0132]    The derivative can be calculated by continuously sampling data from the sensor, and let the control unit process the data according to suitable algorithms known per se, so as to produce a concentration curve and from that curve determine the derivative at each point in time.  
         [0133]    A simpler variant is to measure the difference between consecutive data, possibly after forming a running average over a few data points, and when the difference is stable within some fluctuation interval, the sterilization is regarded to be completed.  
         [0134]    In some instances when the internal channel system of an apparatus, e.g. a dialysis apparatus, is to be disinfected or sterilized (in this case, the channels form the object to be disinfected sterilized, more precisely the inner walls of the channels), the length of the tubings etc renders it difficult to monitor the ozone consumption during the treatment procedure in an accurate manner. Therefore, in accordance with a further embodiment of the invention, the treatment (sterilization or disinfection) is carried out in a plurality of steps or segments. This is schematically illustrated in FIG. 11.  
         [0135]    Thus, the apparatus for the production of ozone water is the same as for the other embodiments disclosed previously. However, as can be seen in FIG. 11, the treatment may be carried out in separate segments A, B and C. These segments could be fractions of tubing lengths in a dialysis apparatus, or separate segments of internal channels or passages in such an apparatus. Of course any kind of apparatus that needs to be sterilized or disinfected could be connected to the treatment system. In this case, i.e. where the ozone generation and detection system is connected to an apparatus and not to a dedicated treatment chamber for sterilization/disinfection, there must be provided means on that apparatus for connecting the ozone water supply tubings, and tubings for removal of ozone water, associated with the production apparatus.  
         [0136]    The operation of this multi stage treatment process is as follows.  
         [0137]    The apparatus or system that requires internal disinfection or sterilization, should be adapted so as to be connectable to the ozone generation and monitoring system according to the invention. This is done by segmenting the internal channels and passages in appropriate stretches of tubing or channels, such that treatment of the entire segment can be done in a reasonable time. If too long passages are exposed to the ozone the time needed may be so long that in fact the construction materials may be adversely affected by the ozone.  
         [0138]    Let us assume that the apparatus has been segmented in three segments as shown in FIG. 11. Then, the valve V 30  is set to feed the ozone water from the generator  1100  through conduit  1102  and into segments A, B and C. In the shown embodiment the generator system sensor  1109  is used to measure the initial ozone concentration, and a sensor  1110  is used to measure the concentration of spent ozone water at the outlet from A.  
         [0139]    When detectors  1109  and  1110  signals that segment A has been properly sterilized, valve V 30  is set to direct the liquid into conduit  1105  and further valve V 40  is set to direct the liquid into segment B via conduit  1103 , thus the flow now is through segments B and C only. The measuring procedure is repeated, now by means of sensor  1111  (in conjunction with sensor  1109  of course), until also segment B is properly sterilized. Valve V 40  is set to direct the flow into conduit  1106 , and valve V 50  directs the flow into conduit  1104 . The procedure is then also repeated for segment C, using sensor  1112  to detect the status of sterilization/disinfection.  
         [0140]    It is also possible to build a system having a plurality of sterilization chambers A, B, C for conducting consecutive treatment of a plurality of devices or objects.