Abstract:
Broadly this invention provides inbred corn line ZS01602. The methods for producing a corn plant by crossing the inbred line ZS01602 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01602 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01602 with at least one other corn line.

Description:
FIELD OF THE INVENTION 
     This invention is in the field of corn breeding, specifically relating to an inbred corn line designated ZS01602. 
     BACKGROUND OF THE INVENTION 
     The original maize plant was indigenous to the Western Hemisphere. The plants were weedlike and only through the efforts of early breeders was a cultivated crop species developed. The physical traits of maize are such that self pollination or cross pollination can occur. Each plant has a separate male and female flower, the tassel and ear, respectively. Natural pollination occurs when wind transfers pollen from tassel to the silks on the corn ears. This type of pollination contributed to the wide variation of maize varieties present in the Western Hemisphere. 
     The development of a planned breeding program for maize only occurred in the last century. Originally, maize was an open pollinated variety having heterogeneous genotypes. The maize farmer selected uniform ears from the yield of these genotypes and reserved them for planting the next season. The result was a field of maize plants that were segregating for a variety of traits. This type of maize selection lead to at most incremental increases in seed yield. 
     Large increases in seed yield were the result of the development of hybrid corn varieties in planned breeding programs. Hybrids were developed by selecting corn lines and selfing these lines for several generations to develop homozygous pure inbred lines and crossing selected inbred lines with unrelated inbred lines to produce hybrid progeny (F1). Inbred lines can be difficult to produce since the inbreeding process in corn decreases the vigor. However, when two inbred lines are crossed, the hybrid plant evidences greatly increased vigor compared to open pollinated segregating maize plants. An important factor of the homozygosity and the homogeneity of the inbred lines is that the hybrid from any cross will always be the same, and can be reproduced. 
     The ultimate objective of the commercial maize seed companies is to produce high yielding, agronomically sound plants which perform well in certain regions or areas of the Corn Belt. To produce these types of hybrids, the companies must develop inbreds which carry needed traits into the hybrid combination. Hybrids are not uniformly adapted for the Corn Belt, but are specifically adapted for regions of the Corn Belt. Northern regions of the Corn Belt require shorter season hybrids than do southern regions of the Corn Belt. Hybrids that grow well in Colorado and Nebraska soils may not flourish in rich Illinois soil. Thus, a variety of major agronomic traits are important in hybrid combination for the various Corn Belt regions, and have an impact on hybrid performance. 
     Inbred line development and hybrid testing have been emphasized in the past half century in commercial maize production as a means to increase hybrid performance. Inbred development is usually done by pedigree selection. Pedigree selection can be selection in an F 2  population produced from a planned cross of two genotypes (often elite inbred lines), or selection of progeny of synthetic varieties, open pollinated, composite, or backcross populations. This type of selection is effective for highly inheritable traits, but other traits, for example, yield requires replicated test crosses at a variety of stages for accurate selection. 
     Maize breeders select for a variety of traits in inbreds that impact hybrid performance along with selecting for acceptable parental traits. Such traits include yield potential in hybrid combination; dry down; maturity; grain moisture at harvest; greensnap; resistance to root lodging; resistance to stalk lodging; grain quality; disease and insect resistance; ear and plant height; performance in different soil types such as: low level of organic matter, clay, sand, black, high pH, low pH; performance in: wet environments, drought environments, and no tillage conditions. These traits appear to be governed by a complex genetic system that makes selection and breeding of an inbred line extremely difficult. Even if an inbred in hybrid combination has excellent yield (a desired characteristic), it may not be useful because it fails to have acceptable parental traits such as seed yield, seed size, pollen production, good silks, plant height, etc. 
     To illustrate the difficulty of breeding and developing inbred lines, the following example is given. Two inbreds compared for similarity of 29 traits differed significantly for 18 traits between the two lines. If 18 simply inherited single gene traits were polymorphic with gene frequencies of 0.5 in the parental lines, and assuming independent segregation (as would essentially be the case if each trait resided on a different chromosome arm), then the specific combination of these traits as embodied in an inbred would only be expected to become fixed at a rate of one in 262,144 possible homozygous genetic combinations. Selection of the specific inbred combination is also influenced by the specific selection environment on many of these 18 traits which makes the probability of obtaining this one inbred even more remote. Thus, the general procedure of producing a non segregating F 1  generation and self pollinating to produce a F 2  generation that segregates for traits does not easily lead to a useful inbred. Great care and breeder expertise must be used in selection of breeding material to continue to increase yield and agronomics of inbreds and resultant commercial hybrids. 
     SUMMARY OF THE INVENTION 
     The present invention relates to an inbred corn line ZS01602. Specifically, this invention relates to plants and seeds of this line. Additionally, this relates to a method of producing hybrid seed corn from this inbred. More particularly, this invention relates to the unique combination of traits that combine in corn line ZS01602. 
     Generally then, broadly the present invention includes an inbred corn seed designated ZS01602. This seed produces a corn plant. 
     The invention also includes the tissue culture of regenerable cells of ZS01602 wherein the tissue regenerates plants having the genotype of ZS01602. The tissue culture is selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks and stalks, and cells and protoplasts thereof. The corn plant regenerated from ZS01602 having ZS01602&#39;s genotype. 
     The invention extends to hybrid seed produced by planting, in pollinating proximity, seeds of corn inbred lines ZS01602 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; and harvesting seeds produced on plants of the inbred. The hybrid seed produced by hybrid combination of plants of inbred corn seed designated ZS01602 and plants of another inbred line. Hybrid plants grown from this hybrid seed. 
     The invention further includes a method of hybrid F1 production. A first generation (F1) hybrid corn plant produced by the process of planting, in pollinating proximity, seeds of corn inbred lines ZS01602 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; harvesting seeds produced on plants of the inbred; and growing a harvested seed. 
     A tissue culture of the regenerable cells of hybrid plants produced with use of ZS01602 genetic material. A tissue culture of the regenerable cells of the corn plant produced by the method described above. 
     DEFINITIONS 
     In the description and examples which follow, a number of terms are used. In order to provide a clear and consistent understanding of the specifications and claims, including the scope to be given such terms, the following definitions are provided. 
     BL MOIST 
     The moisture percentage of the grain at black layer, i.e., when 50% of the plants per plot have reached physiological maturity. 
     COLD GERM 
     Cold Germ is a measurement of seed germination under cold soil conditions. Data is reported as percent of seed germinating. 
     ECB 
     European corn borer a maize eating insect. ECBI is the first brood generation of European corn borers. ECBII is the second generation of European corn borers. 
     EMERGE 
     The number of emerged plants per plot (planted at the same seedling rate) collected when plants have two fully developed leaves. 
     GI 
     This is a selection index which provides a single quantitative measure of the worth of a hybrid based on four traits. Yield is the primary trait contributing to index values. The GI value is calculated by combining stalk lodging, root lodging, yield and dropped ears according to the attached mathematical formula: 
     
         GI=100+0.5(YLD)-0.09(%STALK LODGE)-0.9(%ROOT LODGE)-2.7(%DROPPED EAR) 
    
     GLS 
     Gray Leaf Spot (Cercospora zeae) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     GW 
     Goss&#39; Wilt (Corynebacterium nebraskense). This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     HEATP10 
     The number of Growing Degree Units (GDU&#39;s) or heat units required for an inbred line or hybrid to have approximately 10 percent of the plants shedding pollen. This trait is measured from the time of planting. Growing Degree Units are calculated by the Barger Method where the GDU&#39;s for a 24 hour period are: ##EQU1## The highest maximum temperature used is 86° F. and the lowest minimum temperature used is 50° F. For each inbred or hybrid it takes a certain number of GDU&#39;s to reach various stages of plant development. 
     HEATBL 
     The number of GDU&#39;s after planting when approximately 50 percent of the inbred or hybrid plants in a plot have grain which has reached physiological maturity (black layer). 
     HEATPEEK 
     The number of GDU&#39;s after planting of an inbred when approximately 50 percent of the plants show visible tassel extension. 
     HEATP50 or HTP50 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 50 percent of the plants shedding pollen. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATP90 
     The number of GDU&#39;s accumulated from planting when the last 100 percent of plants in an inbred or hybrid are still shedding enough viable pollen for pollination to occur. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS10 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 10 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS50 or HTS50 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 50 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS90 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 90 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     MDMV A   
     Maize Dwarf Mosaic Virus strain A. The corn is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     MDMV B   
     Maize Dwarf Mosaic Virus strain B. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible and a &#34;9&#34; being very resistant.* 
     MOISTURE 
     The average percentage grain moisture of an inbred or hybrid at harvest time. 
     NLB 
     Northern Leaf Blight (Exserohilum turcicum) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     PCT TILLER 
     The total number of tillers per plot divided by the total number of plants per plot. 
     PLANT 
     This term includes plant cells, plant protoplasts, plant cell tissue cultures from which corn plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like. 
     PLANT HEIGHT 
     The distance in centimeters from ground level to the base of the tassel peduncle. 
     RM 
     Predicted relative maturity based on the moisture percentage of the grain at harvest. This rating is based on known set of checks and utilizes standard linear regression analyses and is referred to as the Minnesota Relative Maturity Rating System. 
     SHED 
     The volume of pollen shed by the male flower rated on a 1-9 scale where a &#34;1&#34; is a very light pollen shedder, a &#34;4.5&#34; is a moderate shedder, and a &#34;9&#34; is a very heavy shedder. If the Table(s) 3 have reduced the 1-9 shed scale to a 1-3 shed scale then any shed Table 3 can be multiplied by 3 to reach the 1-9 shed scale. 
     SLB 
     Southern Leaf Blight (Bipolaris maydis) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     TWT 
     The measure of the weight of grain in pounds for a one bushel volume adjusted for percent grain moisture. 
     VIGOR 
     Visual rating of 1 to 9 made 2-3 weeks post-emergence where a &#34;1 &#34; indicates very poor early plant development, and a &#34;9&#34; indicates superior plant development. 
     WARM GERM 
     A measurement of seed germination under ideal (warm, moist) conditions. Data is reported as percent of seeds germinating. 
     YIELD (YLD) 
     Actual yield of grain at harvest adjusted to 15.5% moisture. Measurements are reported in bushels per acre. 
     % DROPPED EARS (DE) 
     The number of plants per plot which dropped their primary ear divided by the total number of plants per plot. 
     % LRG FLAT 
     Percentage by weight of shelled corn that passes through a 26/64 inch round screen and a 14/64 inch slot screen, but does not pass through a screen with 20.5/64 inch round openings. 
     % LRG ROUND 
     Percentage by weight of shelled corn that passes through a 26/64 inch round screen, but does not pass through a 14/64 inch slot screen or a screen with 20.5/64 inch round openings. 
     % MED FLAT 
     Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen and a 13/64 inch slotted screen, but does not pass through a screen with 17/64 inch round openings. 
     % MED ROUND 
     Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen, but does not pass through a 13/64 inch slot screen or a screen with 17/64 inch round openings. 
     % SML FLAT 
     Percentage by weight of shelled corn that passes through a 17/64 inch round screen and a 12/64 inch slotted screen, but does not pass through a screen with 15/64 inch round openings. 
     %SML ROUND 
     Percentage by weight of shelled corn that passes through a 17/64 inch round screen, but does not pass through a 12/64 inch slotted screen or a screen with 15/64 inch round openings. 
     % ROOT LODGE (RL) 
     Percentage of plants per plot leaning more that 30 degrees from vertical divided by total plants per plot. 
     % STALK LODGE (SL) 
     Percentage of plants per plot with the stalk broken below the primary ear node divided by the total plants per plot. 
     * Resistant--on a scale of 1-9 with 9 evidencing the trait most strongly: 1-2.9 ratings are susceptible, 3-5.9 ratings are intermediate, and 6-9 ratings are resistant. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     ZS01602 can be used as a female or a male line due to its pollen shed and seed production abilities. This ZS01602 line evidences good general combining ability and specific combining ability especially with Lancasters. This line evidences good stability and heat stress tolerances. 
     The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. Most of the data in the Variety Description information was collected at Slater, Iowa. 
     The inbred has been self-pollinated for a sufficient number of generations to give inbred uniformity. During plant selection in each generation, the uniformity of plant type was selected to ensure homozygosity and phenotypic stability. The line has been increased in isolated farmland environments with data on uniformity and agronomic traits being observed to assure uniformity and stability. No variant traits have been observed or are expected in ZS01602. 
     The best method of producing the invention, ZS01602 which is substantially homozygous, is by planting the seed of ZS01602 which is substantially homozygous and self-pollinating or sib pollinating the resultant plant in an isolated environment, and harvesting the resultant seed or the resultant pollen. 
     
                       TABLE 1______________________________________ZS01602VARIETY DESCRIPTION INFORMATION______________________________________#1      Type: Dent#2      Region Best Adapted: South and southeastern regions______________________________________ 
    
     
         __________________________________________________________________________1995 PROJECT MO PVP TRAITSINBRED ZS01602__________________________________________________________________________                 N  MEAN                        STD.                            T-STAT                                 PROB                                      95%  CI__________________________________________________________________________EAR HEIGHT (CM)       15 53.33                        4.58                            45.13                                 0.0000                                     (51.02, 55.65)LENGTH OF PRIMARY EAR LEAF (CM)                 15 87.12                        2.54                            133.0                                 0.0000                                     (85.84, 88.40)WIDTH OF PRIMARY EAR LEAF (CM)                 15 9.41                        0.44                            82.51                                 0.0000                                     (9.18, 9.63)TOP EAR INTERNODE (CM)                 15 12.50                        1.18                            41.02                                 0.0000                                     (11.90, 13.10)DEGREE OF LEAF ANGLE  15 12.80                        2.01                            24.70                                 0.0000                                     (11.78, 13.82)# OF EARS PER PLANT   15 1.33                        0.49                            10.58                                 0.0000                                     (1.09, 1.58)# OF LEAVES ABOVE TOP EAR                 15 5.67                        0.49                            44.98                                 0.0000                                     (5.42, 5.91)# OF PRIMARY LATERAL TASSEL BRANCHES                 15 4.53                        0.83                            21.06                                 0.0000                                     (4.11, 4.96)PLANT HEIGHT (CM)     15 142.9                        10.94                            50.58                                 0.0000                                     (137.3, 148.4)TASSEL LENGTH (CM)    15 44.87                        1.81                            96.14                                 0.0000                                     (43.95, 45.78)TASSEL BRANCH ANGLE   15 10.47                        4.79                            8.46 0.0000                                      (8.04, 12.89)# OF TILLER PER PLANTS                 15 0.27                        0.46                            2.26 0.0406                                     (0.04, 0.50)WEIGHT PER 100 KERNELS (GM)                 15 22.97                        2.70                            32.97                                 0.0000                                     (21.61, 24.34)EAR LENGTH (CM)       15 14.83                        1.23                            46.54                                 0.0000                                     (14.21, 15.46)EAR WEIGHT (GM)       15 113.0                        15.48                            28.27                                 0.0000                                     (105.2, 120.8)# OF KERNEL ROWS      15 17.20                        2.70                            24.63                                 0.0000                                     (15.83, 18.57)COB DIAMETER AT MID-POINT (MM)                 15 25.13                        0.74                            131.0                                 0.0000                                     (24.76, 25.51)EAR DIAMETER AT MID-POINT (MM)                 15 41.27                        1.39                            115.2                                 0.0000                                     (40.56, 41.97)KERNEL LENGTH (MM)    15 9.60                        0.83                            44.90                                 0.0000                                      (9.18, 10.02)KERNEL THICKNESS (MM) 15 5.27                        0.88                            23.08                                 0.0000                                     (4.82, 5.71)KERNEL WIDTH (MM)     15 7.33                        0.82                            34.79                                 0.0000                                     (6.92, 7.75)% ROUND KERNELS (SHAPE GRADE)                 15 32.80                        12.51                            10.16                                 0.0000                                     (26.47, 39.13)SHANK LENGTH          15 11.20                        1.61                            26.90                                 0.0000                                     (10.38, 12.02)__________________________________________________________________________INBREDMO:ZS01602__________________________________________________________________________#3 MATURITYHEATDAYS UNITS63   1534 FROM PLANTING TO 50% OF PLANTS IN SILK61   1481 FROM PLANTING TO 50% OF PLANTS IN POLLEN 9        FROM 10% TO 90% POLLEN SHED#4 PLANTDATA 3        ANTHOCYANIN OF BRACE ROOTS: 1 = ABSENT 2 = FAINT 3 = MODERATE 4     = DARK#5 LEAFCOLOR/DATA 3/DARK GREEN     LEAF COLOR **MUNSELL CODE-5GY 4/4 3        LEAF SHEATH PUBESCENCE (1 = NONE TO 9 = PEACH FUZZ) 5        MARGINAL WAVES (1 = NONE TO 9 = MANY) 3        LONGITUDINAL CREASES (1 = NONE TO 9 = MANY)#6 TASSELCOLOR/DATA 4        POLLEN SHED (0 = STERILE TO 9 = HEAVY SHEDDER) 6/PALE YELLOW     ANTHER COLOR **MUNSELL CODE-5Y 8/9 2/MEDIUM GREEN     GLUME COLOR **MUNSELL CODE-5GY 5/8 W/S 2.5R 4/8 1        BAR GLUME: 1 = ABSENT 2 = PRESENT#7A EAR (HUSKED DATA)COLOR/DATA 6/PALE YELLOW     SILK COLOR (3 DAYS AFTER EMERGE) **MUNSELL CODE-5Y 8/6 2/MEDIUM GREEN     FRESH HUSK (25 DAYS AFTER 50% SILK) **MUNSELL CODE-5GY 6/621/BUFF   DRY HUSK COLOR (65 DAYS AFTER 50% SILK **MUNSELL CODE-2.5Y 8/3 1        POSITION OF EAR AT DRY HUSK: 1 = UPRIGHT 2 = HORIZONTAL 3 =     PENDENT 5        HUSK TIGHTNESS (1 = VERY LOOSE TO 9 = VERY TIGHT) 4        HUSK EXTENSION AT HARVEST: 1 = EXPOSED EAR 2 = 8 CM 3 = 8-10 CM     4 =&gt; 10 CM#7B EAR (HUSKED DATA)DATA 2        KERNEL ROWS: 1 = INDISTINCT 2 = DISTINCT 1        ROW ALIGNMENT: 1 = STRAIT 2 = SLIGHT CURVE 3 = SPIRAL 1        EAR TAPPER: 1 = STRAIT 2 = AVERAGE 3 = EXTREME#8 KERNEL (DRY)COLOR/DATA 1        ALEURONE COLOR PATTERN: 1 = HOMO 2 = SEG 7/YELLOW ALEURONE COLOR **MUNSELL CODE-2.5Y 8/10 7/YELLOW HARD ENDOSPERM COLOR **MUNSELL CODE-2.5Y 7/10 3        ENDOSPERM TYPE 7/YELLOW CROWN COLOR **MUNSELL CODE-2.5Y 8/10#9 COBCOLOR12/LIGHT RED     COB COLOR **MUNSELL CODE-10R 6/6#11 DISEASE RESISTANCENorthern leaf blight = 4.5NLSr2* = 5.5Gray leaf spot = 4.5Eye = 5GW = 6.5MDMVB = 1__________________________________________________________________________ * Northern Leaf Spot Race Two 
    
     The genetically related line to ZS01602 is Holden&#39;s Inbred #117 . Other lines which are comparable in usage or maturity or genetically are B73 and ZS0441 PVP-0200038. 
     The Munsell code is a reference book of color which is known and used in the industry and by persons with ordinary skill in the art of plant breeding. 
     The purity and homozygosity of inbred ZS01602 is constantly being tracked using isozyme genotypes as shown in Table 2. 
     Isozyme Genotypes for ZS01602 
     Isozyme data were generated for inbred corn line ZS01602 according to procedures known and published in the art. The data in Table 2 gives the electrophoresis data on ZS01602. 
     
                                           TABLE 2__________________________________________________________________________ELECTROPHORESIS RESULTS FOR ZS01602__________________________________________________________________________INBREDACP1    ACP4        ADH           MDH1               MDH2                   PGD1                       PGD2                           PH1                              PGM                                 IDH2__________________________________________________________________________ZS0160211  22  22 22  11  11  11  22 22 22__________________________________________________________________________ 
    
     Inbred and Hybrid Performance of ZS01602 
     The traits and characteristics of inbred corn line ZS01602 are listed to compare with other inbreds and/or in hybrid combination. ZS01602 data shows the characteristics and traits of importance, giving a snapshot of ZS01602. 
     Table 3A shows a comparison between ZS01602 and B73. ZS01602 has significantly lower ear placement than does B73. ZS01602 has higher yield and lower grain moisture at harvest than does B73. ZS01602 flowers slightly earlier than B73 across all pollination and silking data. ZS01602 reaches heat peek at the same number of heat units than does B73. ZS01602 has slightly higher germination under cold testing conditions than does B73. 
     
                                           TABLE 3A__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                 PCT  PLANT                           EAR         EAR   PCTYEAR  INBRED      VIGOR EMERGE                 TILLER                      HEIGHT                           HEIGHT                                SHED   QUALITY                                             BARREN__________________________________________________________________________OVERALL ZS01602      7.0   92.5      172.1                           79.4 7.8 B73  6.1   86.7      180.0                           100  8.0 # EXPTS      4     4         4    4    4 DIFF 0.9   5.8       7.9  20.6 0.3 PROB 0.133 0.230     0.157                           0.064***                                0.638__________________________________________________________________________YEAR  INBRED      HEATP10            HEATP50                 HEATP90                      HEATS10                           HEATS50                                HEATS90__________________________________________________________________________OVERALL ZS01602      1530  1558 1627 1564 1581 1618 B73  1551  1591 1668 1592 1626 1661 # EXPTS      4     4    4    4    4    4 DIFF 21    33   40   28   44   43 PROB 0.319 0.257                 0.186                      0.253                           0.155                                0.157__________________________________________________________________________                 BL   % ROOT                           % STALK                                % DROPPEDYEAR  INBRED      HEATPEEK            HEATBL                 MOIST                      LODGE                           LODGE                                EARS   MOISTURE                                             YIELD__________________________________________________________________________OVERALL ZS01602      1496  2734 31.0                  11.9  110.4 B73  1496  2652 29.5                  13.5  99.0 # EXPTS      4     1    1                     4     4 DIFF 0     82   1.5                   1.5   11.4 PROB 0.995                            0.009*__________________________________________________________________________      WARM  COLD % LRG                      % LRG                           % MED                                % MED  % SML % SMLYEAR  INBRED      GERM  GERM ROUND                      FLAT ROUND                                FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01602      96.6  92.0 11.6 13.1 25.2 36.2   5.0   6.7 B73  97.9  89.1 5.5  6.0  28.2 43.0   7.0   8.8 # EXPTS      4     4    4    4    4    4      4     4 DIFF 1.3   2.9  6.1  7.1  3.0  6.8    2.1   2.2 PROB 0.231 0.575                 0.036**                      0.006*                           0.372__________________________________________________________________________ 
    
     Table 3B compares ZS01602 with Holden&#39;s Inbred #117. ZS01602 has better yield by 22 bushels and has lower grain moisture at harvest than does Holden&#39;s Inbred #117 . ZS01602 has slightly more seedling vigor and is a significantly taller plant with lower ear placement than is inbred Holden&#39;s Inbred #117. ZS01602 has slightly higher cold germination results in testing than does Holden&#39;s Inbred #117. ZS01602 flower at significantly lower numbers of heat units across all silking and pollination data. 
     
                                           TABLE 3B__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                 PCT  PLANT                           EAR         EAR   PCTYEAR  INBRED      VIGOR EMERGE                 TILLER                      HEIGHT                           HEIGHT                                SHED   QUALITY                                             BARREN__________________________________________________________________________OVERALL ZS01602      7.0   92.5      172.1                           79.4 7.8 Holden&#39;s      6.5   89.7      156.5                           82.5 8.0 Inbred #117 # EXPTS      4     4         4    4    4 DIFF 0.5   2.8       15.6 3.2  0.3 PROB 0.423 0.408     0.029**                           0.564                                0.391__________________________________________________________________________YEAR  INBRED      HEATP10            HEATP50                 HEATP90                      HEATS10                           HEATS50                                HEATS90__________________________________________________________________________OVERALL ZS01602      1530  1558 1627 1564 1581 1618 Holden&#39;s      1625  1668 1747 1655 1695 1754 Inbred #117 # EXPTS      4     4    4    4    4    4 DIFF 96    109  120  91   113  136 PROB 0.014**            0.003*                 0.004*                      0.011**                           0.011**                                0.000*__________________________________________________________________________                 BL   % ROOT                           % STALK                                % DROPPEDYEAR  INBRED      HEATPEEK            HEATBL                 MOIST                      LODGE                           LODGE                                EARS   MOISTURE                                             YIELD__________________________________________________________________________OVERALL ZS01602      1496  2734 31.0                  11.9  110.4 Holden&#39;s      1537  2702 32.5                  13.5  87.6 Inbred #117 # EXPTS      4     1    1                     4     4 DIFF 40    32   1.5                   1.5   22.8 PROB 0.113                            0.179__________________________________________________________________________      WARM  COLD % LRG                      % LRG                           % MED                                % MED  % SML % SMLYEAR  INBRED      GERM  GERM ROUND                      FLAT ROUND                                FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01602      96.6  92.0 11.6 13.1 25.2 36.2   5.0   6.7 Holden&#39;s      96.2  84.0 5.1  7.2  32.4 40.7   7.5   5.2 Inbred #117 # EXPTS      4     4    4    4    4    4      4     4 DIFF 0.4   8.0  6.5  5.9  7.2  4.5    2.5   1.5 PROB 0.536 0.227                 0.002*                      0.055***                           0.184__________________________________________________________________________ 
    
     Table 3C is an inbred comparison between ZS01602 and ZS0441. ZS01602 has significantly better pollen shed than does ZS0441 as well as having better emergence of plants. ZS01602 is significantly later to reach HeatS 10 and HeatS50 than is ZS0441. ZS01602 reaches both HeatPEEK and HeatBL significantly later than ZS0441. ZS01602 reaches all three rated levels of pollination (HeatP10-90) approximately the same time as ZS0441. The two inbreds are the same in yield and moisture. 
     
                                           TABLE 3C__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                 PCT  PLANT                           EAR         EAR   PCTYEAR  INBRED      VIGOR EMERGE                 TILLER                      HEIGHT                           HEIGHT                                SHED   QUALITY                                             BARREN__________________________________________________________________________OVERALL ZS01602      6.5   87.7      167.3                           69.1 6.8 ZS0441      6.5   85.7      163.9                           68.7 5.9 # EXPTS      15    15        15   15   13 DIFF 0.1   2.0       3.4  0.4  0.9 PROB 0.862 0.497     0.156                           0.857                                0.008*__________________________________________________________________________YEAR  INBRED      HEATP10            HEATP50                 HEATP90                      HEATS10                           HEATS50                                HEATS90__________________________________________________________________________OVERALL ZS01602      1466  1512 1619 1532 1566 1613 ZS0441      1462  1512 1634 1475 1530 1596 # EXPTS      13    13   13   13   13   13 DIFF 4     0    14   57   36   17 PROB 0.709 0.993                 0.301                      0.001*                           0.024**                                0.379__________________________________________________________________________                 BL   % ROOT                           % STALK                                % DROPPEDYEAR  INBRED      HEATPEEK            HEATBL                 MOIST                      LODGE                           LODGE                                EARS   MOISTURE                                             YIELD__________________________________________________________________________OVERALL ZS01602      1431  2673 31.0                  12.9  78.8 ZS0441      1383  2636 27.5                  12.9  79.1 # EXPTS      13    3    1                     15    15 DIFF 48    36   3.5                   0.0   0.4 PROB 0.000*            0.050**                    0.928__________________________________________________________________________      WARM  COLD % LRG                      % LRG                           % MED                                % MED  % SML % SMLYEAR  INBRED      GERM  GERM ROUND                      FLAT ROUND                                FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01602      95.2  85.2 9.9  10.0 27.0 36.7   6.9   7.7 ZS0441      94.5  80.8 14.7 27.9 24.0 28.4   2.9   1.2 # EXPTS      10    10   13   13   13   13     13    13 DIFF 0.6   4.4  4.8  18.0 2.9  8.3    4.1   6.5 PROB 0.500 0.172                 0.024**                      0.000*                           0.139__________________________________________________________________________ 
    
     Table 4A shows the GCA (general combining ability) estimates of ZS01602 compared with the GCA estimates of the other inbreds. The estimates show the general combining ability is weighted by the number of experiment/location combinations in which the specific hybrid combination occurs. The interpretation of the data for all traits is that a positive comparison is a practical advantage. A negative comparison is a practical disadvantage. The general combining ability of an inbred is clearly evidenced by the results of the general combining ability estimates. This data compares the inbred parent in a number of hybrid combinations to a group of &#34;checks&#34;. The check data is from other companies&#39; hybrids, particularly the leader in the industry and ICI Seeds&#39; commercial products and pre-commercial hybrids which were grown in the same sets and locations. 
     Table 4A shows ZS01602 crossed to 308 different inbreds. ZS01602 in hybrid combination shows an advantage for yield. ZS01602 has a slight disadvantage for moisture at -1.5. ZS01602 has less tendency to have stalk lodging and root lodging or dropped ears than the checks. 
     ZS01602 in random hybrid combinations has the GCA shown in Table 4A. When only hybrids containing Lancaster material are analyzed, it becomes clear that ZS01602 has specific combining ability with this type of heterotic pattern of maize. 
     
                                           TABLE 4A__________________________________________________________________________GCA &amp; SCA ESTIMATESADVANTAGES OVER COMMERCIALS &amp; N&#39;SENT     N93      N94         N95            N  FI V M                     GI                       I VLD                            MST                               % SL                                  % RL                                     % DE                                         TWT                                            POP                                               RM__________________________________________________________________________ZS01602XR =   222      8  73 308               -0.8                  -0.3                     2.6                       3.1                         3.9                            -1.5                               0.6                                  0.1                                     0.0 -0.6                                              14                                               124XH =         39               -1.6                  -0.4                     1.8                       3.5                         2.9                            -1.5                               0.3                                  0.0                                     0.0 -0.7                                              19                                               124XT =         6 0.9                  -0.0                     3.4                       4.1                         6.2                            -1.1                               0.3                                  0.1                                     0.0 -0.4                                            -52                                               124__________________________________________________________________________ XR = GCA ESTIMATE: WEIGHTED BY EXPT XH = GCA ESTIMATE: WEIGHTED BY PARENT2 XT = SAME AS XH, BUT USING ONLY THOSE PARENT2 WITH TWO YEARS OF DATA 
    
     Table 5 shows the yield response of ZS01602 in hybrid combination in comparison with the plants in the environment around it at the same location. ZS01602 in hybrid combination yields well across all yield levels. This type of stability of yield appears throughout years of testing in different environments. 
     
                       TABLE 5______________________________________YIELD RESPONSEHYBRID         YIELD______________________________________ZS01602/Inbred     78      104    130    157  182    209Environment     75      100    125    150  175    200______________________________________ 
    
     Table 6A shows in positive numbers the advantage the ZS01602 hybrid has over two commercially available ICI Seeds&#39; hybrids. ZS01602 hybrid compares favorably with both 8344 and 8315. ZS01602 in hybrid comparison has better yield results and less dropped ears and better stalks than either commercial line. ZS01602 in hybrid combination has a better G index rating than either commercial hybrid. 
     Additionally, ZS01602 has a slight disadvantage in moisture over the two commercials. However, the overall ZS01602 hybrid carries a strong package of agronomic traits. 
     
                                           TABLE 6A__________________________________________________________________________HYBRID SUMMARYZS01602/INBREDPERFORMANCE DATA__________________________________________________________________________          CORN PAIRED COMPARISONSHYBRID YEAR      TESTS          GI ADV                FI                  ADV                     YLD                        ADV                           MOIST                               ADV                                  SL ADV__________________________________________________________________________ZS01602RE    5   145              7 97                   5 102.9                        3.3                           20.9                               -0.7                                   6.0                                     4.68344 RE    5   138   92    99.6 20.2   10.5RE    5   145              7 97                   5 102.9                        3.3                           20.9                               -0.7                                   6.0                                     4.6RE    5   138   92    99.6 20.2   10.5ZS01602RE    5   145             13 97                  12 102.9                        8.6                           20.9                               -0.4                                   6.0                                     9.08315 RE    5   132   85    94.3 20.5   14.9RE    5   145             13 97                  12 102.9                        8.6                           20.9                               -0.4                                   6.0                                     9.0RE    5   132   85    94.3 20.5   14.9__________________________________________________________________________          CORN PAIRED COMPARISONSHYBRID YEAR      TESTS          RL ADV                DE  ADV                       TWT  ADV                               POP   ADV__________________________________________________________________________ZS01602RE    5   1.6               0.9                0.0 0.0        24385 2098344 RE    5   2.5   0.0            24176RE    5   1.6               0.9                0.0 0.0        24385 209RE    5   2.5   0.0            24176ZS01602RE    5   1.6             -0.2                0.0 0.2        24385  -98315 RE    5   1.4   0.2            24394RE    5   1.6             -0.2                0.0 0.2        24385  -9RE    5   1.4   0.2            24394__________________________________________________________________________ 
    
     Table 7A shows the entomology strengths and weakness of ZS01602 as an inbred. 
     
                       TABLE 7A______________________________________                       EAR    PLANT   ECB1     ECB2       DAMAGE INTEGRITYINBRED  RATING   RATING     RATING RATING______________________________________ZS01602 5.8      5.7        1.5    6.3______________________________________ 
    
     The inbred ZS01602 can be employed as the female or the male plant in a hybrid production field. The female has good seed size and abundant seed. The mail is an excellent pollen shedder. Additionally, this inbred carries heat stress tolerance and good disease resistance. 
     In hybrid combination, ZS01602 carries good yield stability over environments into the hybrid. ZS01602 shows good performance in heat and stress and tends to carry this trait into its hybrids. This inbred adapts well across the Southern region when in hybrid combination. This inbred has fairly good general combining and specific combining ability with Lancasters for yield with most lines. ZS01602 is a versatile line. 
     The foregoing is set forth by way of example and is not intended to limit the scope of the invention. 
     This invention also is directed to methods for producing a corn plant by crossing a first parent corn plant with a second parent corn plant wherein the first or second parent corn plant is an inbred corn plant from the line ZS01602. Further, both first and second parent corn plants can come from the inbred corn line ZS01602. A variety of breeding methods can be selected depending on the mode of reproduction, the trait, the condition of the germplasm. Thus, any such methods using the inbred corn line ZS01602 are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, haploid and anther culturing and the like. 
     Various culturing techniques known to those skilled in the art, such as haploid, transformation, and a host of other conventional and unconventional methods are within the scope of the invention. All plants and plant cells produced using inbred corn line ZS01602 are within the scope of this invention. The invention encompasses the inbred corn line used in crosses with other, different, corn inbreds to produce (F1) corn hybrid seeds and plants with the characteristics that make good hybrids. This invention includes cells which upon growth and differentiation produce corn plants having the physiological and morphological characteristics of the inbred line ZS01602. 
     Duncan, from at least 1985-1988 produced literature on plant regeneration from callus. Both inbred and hybrid callus have resulted in regenerated plants at excellent efficiency rates. Somatic embryogenesis has been performed on various maize tissue such as glume which before the 1980&#39;s was considered unusable for this purpose. The prior art clearly teaches the regeneration of plants from various maize tissues. 
     European Patent Application, publication 160,390, describes tissue culture of corn which can be used by those skilled in the art. Corn tissue culture procedures are also described in the literature as early as 1982. 
     A deposit of at least 2500 seeds of the inbred seed of this invention is maintained by ICI Seeds, 2369 330th Street, Slater, Iowa 50244. Access to this deposit will be available during the pendency of this application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. The Applicant made a deposit one Jun.11, 1997, of at least 2500 seeds of Inbred Corn Line ZS01602 with the American Type Culture Collection (ATCC), located at 12301 Parklawn Drive, Rockville, Md. 20852. The ATCC Accession number is 209116. Additionally, the Applicant has satisfied all of the requirements of C.F.R. 1.801-1.809, including providing an indication of the viability of the sample. The ATCC deposit will be maintained in that depository, which is a public depository, for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. 
     Inbreds designated MBS are available from Mike Brayton Seed in Iowa. Inbreds designated SGI are available from Seed Genetic Inc. in New Jersey. Information on some ZS designations may be available from the PVP office. 
     Accordingly, the present invention has been described with some degree of particularity directed to the preferred embodiment of the present invention. It should be appreciated, though, that the present invention is defined by the following claims construed in light of the prior art so that modifications or changes may be made to the preferred embodiment of the present invention without departing from the inventive concepts contained herein.