Abstract:
The present invention is to obtain novel anti-inflammatory agents having decreased antibacterial activity and increased anti-inflammatory action, and is psedoerythromycin derivatives represented by the following general formula [I],  
                         
 
     wherein R1 and R2 are same or different and each represents H, alkyl, alkynyl, acyl or sulfonyl, in which these groups may optionally have substituents, and Me indicates methyl.

Description:
CROSS REFERENCE TO RELATED APPLICATION  
       [0001]    This application is a Divisional of U.S. patent application Ser. No. 10/088,965 filed on Jul. 25, 2002. application Ser. No. 10/088,965 is the national phase of PCT International Application No. PCT/JP00/05503 filed on Aug. 17, 2000 under 35 U.S.C. § 371. 
     
    
     
       BACKGROUND OF THE INVENTION  
         [0002]    1. Field of the Invention  
           [0003]    The present invention relates to novel pseudoerythromycin derivatives or salt thereof.  
           [0004]    2. Description of Related Art  
           [0005]    Erythromycin (hereinafter sometimes designates as EM) has been used for long time as 14-membered macrolide antibiotic for treatment of infectious disease caused by Gram-positive bacteria. During past ten and several years, erythromycin has known to improve long-term chronic inflammatory diseases such as diffuse panbronchiolitis and bronchial asthma, except for therapeutic action to bacterial infectious diseases. (Kudo, Shoji et al., Studies of clinical results on long term small administration of erythromycin for diffuse panbronchiolitis-Treatment results for 4 years, J. Japan. Thorac. Dis. Assoc., 25:632-642, 1987).  
           [0006]    Erythromycin is an antibiotic and has antibacterial action which is not always required for treatment of inflammatory diseases. Consequently, resistant bacteria are generated in patients who are administered antibiotics, as a result, it causes deterioration for treatment of infectious disease in the other occasion.  
           [0007]    As described above, Kudo, Shoji et al. demonstrated that diffuse panbronchiolitis could be improved by long term small administration of erythromycin (Kudo, Shoji et al., Studies of clinical results on long term small administration of erythromycin for diffuse panbronchiolitis-Treatment results for 4 years, J. Japan. Thorac. Dis. Assoc., 25:632-642, 1987).  
         SUMARRY AND OBJECT OF THE INVENTION  
         [0008]    Recently, actions other than antibiotic activity of erythromycin is noted, as a result, usefulness other than pulmonary region, for example not limited in diffuse panbronchiolitis but for chronic sinusitis and Crohn&#39;s disease are reported. The mechanism of action of erythromycin for chronic disease such as diffuse panbronchiolitis is thought to be the result of original antibacterial action. Research studies are now ongoing, and indicate the antiinflammatory action mediated by immune inflammatory cells in the penumbral chronic respiratory tract inflammation.  
           [0009]    For example, the studies indicate the regulation for migration of neutrophils to infectious region by direct action, and the regulation for migration of neutrophils or for release of active oxygen from neutrophils by indirect action through mediators or cytokines. Further, erythromycin has an action to lymphocytes, macrophages and mast cells to regulate their proliferation or cytokine production, or to induce differentiation. (Kudo, Shoji Ed., Supervisors: Shimizu, Kihachiro and Omura Satoshi “Inflammation, Immunity and Macrolides Up to Date”, Iyaku Journal Inc., Osaka, 1996)  
           [0010]    As explained above, 14-membered macrolides are thought to cure chronic respiratory diseases as a result of exhibiting immune regulation and antiinflammatory action.  
           [0011]    We have aimed at the promoting action of erythromycin for differentiation-induction from monocyte to macrophage (N. Keicho, S. Kudoh, H. Yotsumoto, K. Akagawa, “Erythromycin promotes monocyte to macrophage differentiation”, J. Antibiotics, 47, 80-89, 1994), and tried to synthesize erythromycin derivatives for the purpose of creating the derivatives having disappeared antibacterial action and enhanced promoting action for differentiation-induction.  
           [0012]    The present invention relates to a novel pseudoerythromycin derivative represented by the general formula [I],  
                         
 
           [0013]    wherein R 1  and R 2  are same or different and each represents H, alkyl, alkynyl, acyl, or sulfonyl, in which these groups may optionally have substituents, and Me indicates methyl.  
           [0014]    Further, the present invention relates to a novel pseudoerythromycin derivative represented by the general formula [II],  
                         
 
           [0015]    wherein R is heterocyclic containing N which may optionally have substituents, and Me indicates methyl.  
           [0016]    The present invention further relates to a novel pseudo erythromycin derivative represented by the general formula [III],  
                         
 
           [0017]    wherein R 3  is 0 or NOH, and Me indicates methyl.  
           [0018]    The invention further relates to a novel pseudoerythromycin derivative represented by the general formula [IV],  
                         
 
           [0019]    wherein R 1  and R 2  are same or different and each represents H or methyl, R 3  and R 4  represent H, hydroxyl or amino, and Me indicates methyl.  
           [0020]    The present invention further relates to a novel pseudo erythromycin derivative represented by the general formula [V],  
                         
 
           [0021]    wherein R 1  and R 2  are same or different and each represents H or methyl, and Me indicates methyl.  
           [0022]    Synthetic methods of various erythromycin derivatives are, for example, illustrated in the synthetic scheme as shown in FIG. 1. Namely, erythromycin A is treated with ice-cold acetic acid according to the references: (a) I. O. Kibwage, R. Busson, G. Janssen, J. Hoogmartens, H. Vanderhaeghe, Translactonization of Erythromycins, J. Org. Chem., 52, 990-996, 1987, (b) H. A. Kirst, J. A. Wind, J. W. Paschal, Synthesis of Ring-Constracted Derivatives of Erythromycin, J. Org. Chem., 52, 4359-4362, 1987, introducing to erythromycin A enol ether (EM 201), subsequently refluxing in methanol with heating in the presence of potassium carbonate to introduce pseudoerythromycin A enol ether (EM701) (known compound).  
           [0023]    The product was treated with iodine and sodium acetate according to the reference (L. A. Friberg, U.S. Pat. No. 3,725,385) to obtain de-N-methyl-pseudoerythromycin A enol ether (EM703) (known compound). The compound was further treated with iodine and sodium methoxide to obtain bis(de-N-methyl)-pseudo erythromycin A enol ether (EM721) (novel compound). Alkylation, acylation and sulfonylation using EM703 and EM721 resulted to synthesize various derivatives through bis-de(3′-N-methyl)-3′-N-ethyl-8, 9-anhydro-pseudoerythromycin A 6, 9-hemiketal (EM722).  
           [0024]    The synthetic scheme of compounds of the present invention is illustrated in FIG. 1. Namely, the compounds can be obtained by the synthetic route of: erythromycin A (EMA)→erythromycin A enol ether (EM201)→pseudoerythromycin A enol ether (EM701)→de-N-methyl-pseudoerythromycin A enol ether (EM703)→bis (de-N-methyl)-pseudoerythromycin A enol ether (EM721).  
           [0025]    In order to confirm enhancing effect for differentiation-induction of the compounds of the present invention, the enhancing effect for differentiation-induction from human monocyte to macrophage was assayed. Method is performed as follows.  
           [0026]    THP-1 cells were collected from cultured liquid by centrifugation, adjusted the concentration to 2×10 5  cells/ml using medium (RPMI 1640) and distributed into the 48-well plate at 500 μl/well. PMA solution 10 μl and sample solution 5 μl were added in each well, stirred with mild shaking and incubated at 37° C. for 72-96 hours under 5% CO 2 . Further MTT 0.5 mg/ml solution was added at 300 μl/well, and incubated at 37° C. for 3 hours under 5% CO 2 . Supernatant solution was suctioned using the injection tube, added DMSO 500 μl using automatic continuous injector to dissolve formazan completely and transferred each 100 μl to the 96-well plate. The optical absorption was measured using the plate-reader.  
           [0027]    Results of the enhancing effect for differentiation-induction from human monocyte to macrophage measured by the above assay method are shown in Table 1.  
                                                                                                                                                                                                                                                             TABLE 1                           Structure of EM703 analogous derivatives and activities in THP-1/Mφ system                Others   Treated conc. (μM)                EM   R 1     R 2     0.3   1   3   10   30   ED 50  (μM)*                    703   Me   H   +   +   +   +   /   0.3       721   H   H   NT   NT   −   +   /   10       722   Et   H   −   +   +   ++   /   1       723   Et   Et   −   +   +       /   1       724   Allyl   H   −   +   +   ++   /   1       725   Allyl   Allyl   NT   −   ±   +   /   3       726   Ac   H   −   −   −   −   −   −       727   Ms   Me   −   +   +   +   +   1       728   CH 2 C≡CH   H   −   +   +   +   +   1       729   CH 2 C≡CH   CH 2 C≡CH   −   ±   ±   ±   /   1       730   Pr   H   +   +   +   /   /   0.3       731   Pr   Pr   −   −   +   /   /   3       732   Bn   H   +   +   +   +   /   0.3       733   Bn   Bn   −   ±   ±   /   /   1                                                        734                                 −   ±   +   +   /   1               735                                 −   ±   ++   /   1                    736   i-Pr   H   −   ±   +   ++   /   1       737   Me   Me decladinose   NT   NT   −   +   /   10       738   C 6 H 13     H   −   ±   +   /   /   1       739   C 6 H 13     C 6 H 13     −   ±   +   +   /   1       740   C 2 H 4 F   Me   ±   ±   +   +   +   0.3       742   CH 2 CN   Me   −   −   −   +   +   10       743   Me   Me Cl2oxime   NT   −   ±   −   /   −       744   C 3 H 6 OH   Me   NT   −   −   −   /   −       745   C 2 H 4 OAc   Me   −   −   ++   ++   ++   3       746   Me   Me Cl2MeCHOH   −   ±   +   +   +   1                                                        747                                 NT   NT   −   ±   ++   10               748                                 −   ±   ++   ++   /   1                    749   (CH 2 ) 10 Br   (CH 2 ) 10 Br   NT   ±   +   +   /insolule   1       750   Me   Me Cl2MeCHNH 2     NT   −   −   ±   /   10       751   H   Me Cl2MeCHOH   ±   ±   +   +   /   0.3       754   Me   H decladinose   NT   −   −   NT   +   30       EM   Me   Ml   NT   −   ±   +   +   3       CAM   Me   Ml   NT   NT   −   +   −   10       EM oxim   Me   Me C9oxime   NT   −   ±   ±   ++   3                  
 
           [0028]    In Table 1: Me:methyl; Pr:propyl; Et:ethyl; Ac:acetyl; and Ms:methanesulfonyl. *ED 50 :Drug concentration (μM) required for 50% differentiation-induction of THP in Mφ.  
           [0029]    In Table 1, indicated activity is represented in comparison with enhancing action for differentiation-induction of EM 100 μM, and symbols are: ++: enhanced 100% or more; +: enhanced 50-100%; ±: enhanced 25-50%; −: no activity; /: expressed cytotoxicity; and NT: not tested or under assessment.  
           [0030]    As shown in Table 1, since the smaller the value of ED 50  (μM) (minimum drug concentration required for 50% differentiation-induction from THP-1 to Me), the stronger the differentiation-induction activity, it was found that the compounds of the present invention have enhancing action for differentiation-induction from THP-1 to Mφ.  
           [0031]    Next, the suppressive effect of the compound of the present invention (EM703) against bleomycin-induced pulmonary fibrosis was examined (hereinafter sometimes designates bleomycin as BLM).  
           [0032]    A sample suspended in 5% gum arabic was orally administered, 50 mg/kg/day for 17 days (from day-3 to day-13), and bleomycin, 100 mg/kg, was administered from tail vein in day-0. on day-28, animals were sacrificed under anesthesia and fibrosis of the lungs was compared with non-administered mice. Suppressive effects are shown in Table 2.  
           [0033]    References:  
           [0034]    Azuma A., Furuta T., Enomoto T., Hashimoto Y., Uematsu K., Nukariya N., Murata A., Kudoh S., Preventive effect of erythromycin on experimental bleomycin-induced acute lung injury in rats Thorax 53, 186-189, 1998  
                                     TABLE TWO                           [Administration schedule]                                                         Results: Hydroxyproline levels in tissue                        Assay result   Weight conversion           Group       (μmol/l)   (μmol/g)                       Cont       440   4.0           BLM   1   785   7.1           BLM   2   733   6.4           EM703   1   552   5.0           EM703   2   489   4.6           EM703   3   591   5.4           BLM + EM703   1   583   5.2           BLM + EM703   2   495   4.5           BLM + EM703   3   437   4.4           BLM + EM703   4   314   2.9           BLM + EM703   5                                                                                  
 
           [0035]    As indicated above, hydroxyproline is an index of lung fibrosis and higher value indicates hyperfibrosis. Hydroxyproline level, an index for lung injury, in BLM administered group was reduced in a group of BLM+EM703.  
           [0036]    Next, the suppressive effect of the compound EM703 against pneumonia caused by influenza viral infection was examined.  
           [0037]    Sample was dissolved in physiological saline containing 1% DMSO and amount corresponding to oral dosage of the small administration for long-term therapy was administered from day-1 to day-6 of the infection to mice influenza pneumonia model (0.3 mg and 0.03 mg/mice), once a day, intraperitoneally. Results were compared with control group which was given only solvent.  
           [0038]    Reference:  
           [0039]    Sato K., Suga M., Akaike T. et al., Therapeutic effect of erythromycin on influenza virus-induced lung injury in mice. Am. J. Respir Crit. Care Med. 157, 853-859, 1998.  
           [0040]    Results are shown in FIG. 2 and FIG. 3. In this system, mice developed pneumonia and almost died about 20 days after infection. Contrary to that, as shown in FIG. 2, administration of EM703, 0.3 mg/mice, cured pneumonia and 40% of mice were survived. Further, as shown in FIG. 3, mice without administration of drugs (control) indicated significant decrease of body weight due to pneumonia, but administration of EM703 indicated to increase body weight from day-10. This indicates suppressive effect against pneumonia and result to cure pneumonia.  
           [0041]    As described above, the compound of the present invention shows suppressive effect against influenza virus-induced pneumonia. 
       
    
    
     BRIEF DESCRIPTION OF THE FIGURES  
       [0042]    [0042]FIG. 1 shows an example of the synthetic scheme of the compound of the present invention.  
         [0043]    [0043]FIG. 2 is a graph of the suppressive effect against pneumonia showing relationship between numbers of day after infection due to influenza virus infection and survival rates of the compound of the present invention.  
         [0044]    [0044]FIG. 3 is a graph showing suppressive effect of the compound of the present invention on bleomycin-induced pulmonary fibrosis. 
     
    
     DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS  
       [0045]    The present invention is explained by illustrating referential examples and examples, but is not limited within these examples.  
       REFERENTIAL EXAMPLE 1  
       [0046]    Synthesis of EM701  
         [0047]    Glacial acetic acid solution of erythromycin A (12.4 g, 16.9 mmol) was stirred at room temperature for 2 hours, added slowly aqueous sodium hydrogen carbonate and neutralized. The reaction mixture was extracted with chloroform, dehydrated the organic layer with sodium sulfate, filtered off the sodium sulfate and removed the solvent by distillation to obtain crude substance. The crude substance was purified with silica gel chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM201 (7.7 g, 63%). Subsequently, potassium carbonate (1.4 g, 10.6 mmol) was added to the methanol solution (100 ml) of EM 201 (7.6 g, 10.6 mmol) and refluxed for 2 hours. After distilled off the solvent, the residue was dissolved in aqueous sodium hydrogen carbonate and extracted with chloroform. The mixture was dehydrated with sodium sulfate, filtered and removed the sodium sulfate, then the obtained crude substance was purified by silica gel chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM701 (5.9 g, 78%, white powder).  
       EXAMPLE 1  
       [0048]    Synthesis of De(3′-N-Methyl)-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM703)  
                         
 
         [0049]    Sodium acetate (3.9 g, 48.5 mmol) and iodine (2.5 g, 9.7 mmol) were added in this order to methanol (52.0 mL)-water (13.0 mL) solution of EM701 (6.9 g, 9.7 mmol) at room temperature, and stirred at 50° C. for 3 hours. During the stirring, 1N aqueous solution of sodium hydroxide was added to maintain at pH 8-9 continuously. After confirming the completion of the reaction by TLC, the reaction mixture was diluted with aqueous ammonia (7.5 mL)-water (200 mL), and extracted with dichloromethane. After dehydrating the organic layer with sodium sulfate, the sodium sulfate was removed by filtration and distilled off the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM703 (4.8 g, Yield: 70%, white powder). EM703: m. p.: 177-180° C.  
         [0050]    Example 1 is a known compound. This is shown at line 703 in Table 1.  
       EXAMPLE 2  
       [0051]    Synthesis of Bis-De(3′-N-Methyl)-8, 9-Anhydro-Pseudo Erythromycin A 6, 9-Hemiketal (EM721)  
                         
 
         [0052]    Sodium (4.5 g, 1.67 mmol) was added in methanol (15 mL) to prepare methanol solution of sodium methoxide, and EM703 (195.4 mg, 0.279 mmol) and iodine (353.6 mg, 1.393 mmol) were added in this order at 0° C. and stirred for 3 hours. After confirming completion of the reaction by TLC, sodium thiosulfate (0.8 g), aqueous ammonia (0.5 mL) and water (80 mL) were added and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM721 (166.3 mg, Yield: 87%, white powder).  
                                                                     EM721:   m. p.: 134-136° C.           IR (KBr) ν:   3467.4, 2973.7, 2935.1, 2879.2, 1700.9,               1637.3, 1457.9, 1380.8, 1265.1, 1166.7,               1126.2, 1079.9, 1037.5, 1016.3 cm −1 .           HRMS (FAB) m/z:   C 35 H 61 NO 12 Na [M + Na] +                  Calculated   710.4091,           Found   710.4060.                      
 
       EXAMPLE 3  
       [0053]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-Ethyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM722)  
                         
 
         [0054]    N,N-Diisopropylethylamine (26.6 μL, 0.153 mmol) and ethyl iodide (12.2 μL, 0.153 mmol) were added to dimethylformamide (1.0 mL) solution of EM721 (21.0 mg, 0.0305 mmol) and stirred at room temperature for 4 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM722 (7.0 mg, Yield: 32%, white powder).  
                                                                     EM722:   m. p.: 124-126° C.           IR (KBr) ν:   3471.6, 2933.2, 1704.8, 1457.9, 1378.9,               1263.1, 1166.7, 1128.2, 1074,2, 1037.5,               1018.2 cm −1 .           HRMS (FAB) m/z:   C 37 H 65 NO 12 Na [M + Na] +                  Calculated   738.4404           Found   738.4393.                      
 
       EXAMPLE 4  
       [0055]    Synthesis of Bis-De(3′-N-Methyl)-3′, 3′-N, N-Diethyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM723)  
                         
 
         [0056]    N,N-Diisopropylethylamine (26.6 μL, 0.153 mmol) and ethyl iodide (12.2 μL, 0.153 mmol) were added to dimethylformamide (1.0 mL) solution of EM721 (21.0 mg, 0.0305 mmol) and stirred at room temperature for 4 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM723 (10.3 mg, Yield: 45%, white powder).  
                                                                     EM723:   m. p.: 165-168° C.           IR (KBr) ν:   3473.7, 2935.1, 1699.0, 1382.7, 1317.1,               1267.0, 1166.7, 1126.2, 1108.9, 1078.0,               1016.3 cm −1 .           HRMS (FAB) m/z:   C 39 H 69 NO 12 Na [M + Na] +                  Calculated   766.4717           Found   766.4710.                      
 
       EXAMPLE 5  
       [0057]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-Allyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM724)  
                         
 
         [0058]    Allyl bromide (148.3 μL, 1.714 mmol) was added to dichloromethane (5.7mL) solution of EM721 (117.8 mg, 0.171 mmol) and N,N-Diisopropylethylamine (298.6 μL, 1.714 mmol) at 0° C. and stirred at room temperature for 2 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM724 (21.9 mg, Yield: 30%, white powder) was obtained.  
                                                                     EM724:   m. p.: 106-109° C.           IR (KBr) ν:   3448.8, 2971.8, 2933.2, 1718.3, 1637.3,               1380.8, 1265.1, 1166.7, 1126.2, 1078.0,               1037.5, 1016.3 cm −1 .           HRMS (FAB) m/z:   C 38 H 65 NO 12 Na [M + N] +                  Calculated   750.4404,           Found   750.4420.                      
 
       EXAMPLE 6  
       [0059]    Synthesis of Bis-De(3′-N-Methyl)-3′, 3′-N, N-Diallyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM725)  
                         
 
         [0060]    Allyl bromide (148.3 μL, 1.714 mmol) was added to dichloromethane (5.7 mL) solution of EM721 (117.8 mg, 0.171 mmol) and N,N-Diisopropylethylamine (298.6 μL, 1.714 mmol) at 0° C., stirred at room temperature for 2 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM725 (64.3 mg, Yield: 59%, white powder).  
                                                                     EM725:   m. p.: 140-142° C.           IR (KBr) ν:   3471.7, 2971.8, 2927.4, 1700.9, 1637.3,               1380.8, 1317.1, 1265.1, 1166.7, 1124.3,               1114.7, 1049.1, 1016.3 cm −1 .           HRMS (FAB) m/z:   C 41 H 69 NO 12 Na [M + Na] +                  Calculated   790.4717           Found   790.4716.                      
 
       EXAMPLE 7  
       [0061]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-Acetyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM726)  
                         
 
         [0062]    Acetic anhydride (8.4 μL, 0.0759 mmol) was added to dichloromethane (1.6 mL) solution of EM721 (34.8 mg, 0.0506 mmol) at 0° C., stirred for 10 minutes and further stirred at room temperature for 30 minutes. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol=100:1→20:1) to obtain EM726 (33.4 mg, Yield: 91%, white powder).  
                                                                     EM726:   m. p.: 137-139° C.           IR (KBr) ν:   3417.2, 2973.7, 2935.1, 1699.0, 1454.1,               1376.9, 1317.1, 1268.9, 1166,7, 1124.3,               1076.1, 1033.7, 1018.2, 1000.9 cm −1 .           HRMS (FAB)m/z:   C 37 H 63 NO 13 Na [M + Na] +                  Calculated   752.4197           Found   752.4202.                      
 
       EXAMPLE 8  
       [0063]    Synthesis of De(3′-N-Methyl)-3′-N-Sulfonyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM727)  
                         
 
         [0064]    Methane sulfonyl chloride (9.3 μL, 0.249 mmol) was added to dichloromethane (4.2 ml) solution of EM703 (87.6 mg, 0.125 mmol) at 0° C. and stirred for 3 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol=100:1→20:1) to obtain EM727 (37.2 mg, Yield: 91%, white powder).  
                                                                     EM727:   m. p.: 225-228° C.           IR (KBr) ν:   3497.6, 2973.7, 2935.1, 1704.8, 1463.7,               1380.8, 1326.8, 1319.1, 1265,1, 1166.7,               1141.7, 1074.2, 1041.4, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 37 H 65 NO 14 SNa [M + Na] +                  Calculated   802.4023           Found   802.3995.                      
 
       EXAMPLE 9  
       [0065]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-Propargyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM728)  
                         
 
         [0066]    3-Bromopropine (137.8 μL, 1.546 mmol) was added to dichloromethane (5.2 mL) solution of EM721 (106.3 mg, 0.155 mmol) and N,N-Diisopropylethylamine (269.3 μL, 1.546 mmol), and stirred at room temperature for 24 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM728 (41.3 mg, Yield: 37%, white powder).  
                                                                     EM728:   m. p.: 113-115° C.           IR (KBr) ν:   3413.0, 2973.7, 2935.1, 1706.8, 1457.9,               1382.7, 1263.1, 1166.7, 1126,2, 1078.0,               1039.4, 1016.5 cm −1 .           HRMS (FAB)m/z:   C 38 H 63 NO 12 Na [M + Na] +                  Calculated   748.4248           Found   748.4260.                      
 
       EXAMPLE 10  
       [0067]    Synthesis of Bis-De(3′-N-Methyl)-3′, 3′-N, N-Di-Propargyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM729)  
                         
 
         [0068]    3-Bromopropine (137.8 μL, 1.546 mmol) was added to dichloromethane (5.2mL) solution of EM721 (106.3 mg, 0.155 mmol) and N,N-Diisopropylethylamine (269.3 μL, 1.546 mmol) and stirred at room temperature for 24 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05) to obtain EM729 (27.9 mg, Yield: 24%, white powder).  
                                                     EM729:   m. p.: 123-125° C.       IR (KBr) ν:   3415.0, 3309.2, 2971.8, 2933.2, 2877.3,           1706.7, 1457.9, 1375.0, 1263.1, 1166.7,           1116.6, 1072.2, 1049.1, 1035.6, 1016.3 cm −1 .       HRMS (FAB)m/z:   C 41 H 65 NO 12 Na [M + Na] +                  Calculated   786.4404           Found   786.4404.                      
 
       EXAMPLE 11  
       [0069]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-Propyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM730)  
                         
 
         [0070]    N,N-Diisopropylethylamine (59.6 μL, 0.342 mmol) and 1-iodopropane (33.3 μL, 0.342 mmol) were added in this order to acetinitrile (2.3 mL) solution of EM721 (23.5 mg, 0.0342 mmol) and refluxed at 80° C. for 20 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM730 (5.7 mg, Yield: 23%, white powder).  
                                                                     EM730:   m. p.: 109-111° C.           IR (KBr) ν:   3435.0, 2971.8, 2935.1, 2879.2, 1706.7,               1459.8, 1380.8, 1263.1, 1166.7, 1126.2,               1078.0, 1035.6, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 38 H 67 NO 12 Na [M + Na] +                  Calculated   752.4560           Found   752.4564.                      
 
       EXAMPLE 12  
       [0071]    Synthesis of Bis-De(3′-N-Methyl)-3′, 3′-N, N-Di-Propyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM731)  
                         
 
         [0072]    N,N-Diisopropylethylamine (59.6 μL, 0.342 mmol) and 1-iodopropane (33.3 μL, 0.342 mmol) were added in this order to acetinitrile (2.3 mL) solution of EM721 (23.5 mg, 0.0342 mmol) and refluxed at 80° C. for 20 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM731 (12.0 mg, Yield: 40%, white powder).  
                                                     EM731:   m. p.: 148-151° C.       IR (KBr) ν:   3435.0, 2964.1, 2933.2, 2873.4, 1706.7,           1457.9, 1376.9, 1319.1, 1263.1, 1166.7,           1110.8, 1081.9, 1049.1, 1035.6, 1016.3 cm −1 .       HRMS (FAB)m/z:   C 41 H 73 NO 12 Na [M + Na] +                  Calculated   794.5030           Found   794.5005.                      
 
       EXAMPLE 13  
       [0073]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-Benzyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM732)  
                         
 
         [0074]    Benzyl chloride (297.3 μL, 2.584 mmol) was added to dichloromethane (4.3 mL) solution of EM721 (88.8 mg, 0.129 mmol) and N,N-diisopropylethylamine (450.1 μL, 2.584 mmol) at room temperature and stirred for 96 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM732 (49.9 mg, Yield: 50%, white powder).  
                                                                     EM732:   m. p.: 126-128° C.           IR (KBr) ν:   3410.0, 2971.8, 2935.1, 1706.7, 1456.0,               1378.9, 1263.1, 1166.7, 1124.3, 1078.0,               1049.1, 1039.4, 1016.3, 983.5, 937.2,               808.0, 752.1 cm −1 .           HRMS (FAB)m/z:   C 42 H 67 NO 12 Na [M + Na] +                  Calculated   800.4560           Found   800.4565.                      
 
       EXAMPLE 14  
       [0075]    Synthesis of Bis-De(3′-N-Methyl)-3′, 3′-N,N-Di-Benzyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM733)  
                         
 
         [0076]    N,N-Diisopropylethylamine (135.9 μL, 0.780 mmol) and benzyl chloride (89.7 μL, 0.780 mmol) were added in this order to acetinitrile (1.3 mL) solution of EM721 (26.8 mg, 0.0390 mmol) and refluxed at 80° C. for 60 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM733 (19.6 mg, Yield: 58%, white powder).  
                                                     EM733:   m. p.: 149-152° C.       IR (KBr) ν:   3420.6, 2969.8, 2935.1, 1700.9, 1454.1,           1375.0, 1324.9, 1263.1, 1166.7, 1116.6,           1076.1, 1049.1, 1016.3, 752.1, 700.0 cm −1 .       HRMS (FAB)m/z:   C 49 H 73 NO 12 Na [M + Na] +                  Calculated   890.5030           Found   890.5032                      
 
       EXAMPLE 15  
       [0077]    Synthesis of De(3′-Dimethylamino)-3′-Piperidino-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM734)  
                         
 
         [0078]    N,N-Diisopropylethylamine (42.5 μL, 0.244 mmol) and 1,5-dibromopentane (33.2 μL, 0.244 mmol) were added in this order to acetinitrile (4.9 mL) solution of EM721 (16.8 mg, 0.0244 mmol) and refluxed at 80° C. for 24 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM734 (13.3 mg, Yield: 72%, white powder).  
                                                                     EM734:   m. p.: 128-130° C.           IR (KBr) ν:   3420.0, 2971.8, 2935.1, 2858.0, 1710.6,               1454.1, 1380.8, 1319.1, 1263.1, 1164.8,               1110.8, 1074.2, 1047.2, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 40 H 70 NO 12  [M + Na] +                  Calculated   756.4897           Found   756.4901                      
 
       EXAMPLE 16  
       [0079]    Synthesis of De(3′-Dimethylamino)-3′-Pyrrolidino-8, 9-Anhydro-Pseudoerythromycin A 6, 9-hemiketal (EM735)  
                         
 
         [0080]    N,N-diisopropylethylamine (40.2 μL, 0.231 mmol) and 1,4-dibromobutane (27.6 μL, 0.231 mmol) were added in this order toacetinitrile (4.6 mL) solution of EM721 (15.9 mg, 0.0231 mmol) and refluxed at 80° C. for 24 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:1:0.1) to obtain EM735 (11.9 mg, Yield: 70%, white powder).  
                                                                     EM735:   m. p.: 127-129° C.           IR (KBr) ν:   3420.0, 2971.8, 2937.1, 1702.8, 1457.9,               1382.7, 1265.1, 1166.7, 1124.3, 10761.1,               1049.1, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 39 H 68 NO 12  [M + Na] +                  Calculated   742.4741           Found   742.4743                      
 
       EXAMPLE 17  
       [0081]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-(2-Propyl)-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM736)  
                         
 
         [0082]    N,N-Diisopropylethylamine (459.2 μL, 2.636 mmol) and 2-bromopropane (247.5 μL, 2.636 mmol) were added in this order to acetinitrile (4.4 mL) solution of EM721 (90.6 mg, 0.132 mmol) and stirred at 80° C. for 72 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:1:0.1) to obtain EM736 (25.3 mg, Yield: 26%, white powder). The raw material EM721 was recovered 47.1 mg (Yield: 52%).  
                                                                     EM736:   m. p.: 102-104° C.           IR (KBr) ν:   3420.0, 2971.8, 2933.2, 2877.3, 1718.3,               1459.8, 1380.8, 1263.1, 1166.7, 1126.2,               1078.0, 1049.1, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 38 H 67 NO 12 Na [M + Na] +                  Calculated   752.4560           Found   752.4576.                      
 
         [0083]    [0083] 
         [0084]    Example 17 is a known compound. This is shown at line 736 in Table 1.  
       EXAMPLE 18  
       [0085]    Synthesis of De(3-O-Cladinosyl)-8, 9-Anhydro-Pseudo Erythromycin A 6, 9-Hemiketal (EM737)  
                         
 
         [0086]    p-toluenesulfonic acid monohydrate (80.3 μL, 0.422 mmol) was added to dimethylformamide (5.6 mL) solution of EM701 (201.6 mg, 0.282 mmol) and stirred at 50° C. for 8 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water, adjusted to pH 8.0 by adding saturated aqueous sodium hydrogen carbonate and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM737 (84.7 mg, Yield: 54%, white powder)  
                                                                     EM737:   m. p.: 109-111° C.           IR (KBr) ν:   3486.7, 2973.7, 2937.1, 2877.3, 1708.6,               1631.5, 1457.9, 1382.7, 1265.1, 1164.8,               1110.8, 1076.1, 1039.4 cm −1 .           HRMS (FAB)m/z:   C 29 H 52 NO 9  [M + Na] +                  Calculated   558.3641           Found   558.3616                      
 
       EXAMPLE 19  
       [0087]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-Hexyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM738)  
                         
 
         [0088]    N,N-Diisopropylethylamine (408.5 μL, 2.345 mmol) and 1-bromohexane (328.7 μL, 2.345 mmol) were added in this order to acetinitrile (3.9 mL) solution of EM721 (80.6 mg, 0.117 mmol) and stirred at 60 ° C. for 24 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM738 (33.7 mg, Yield: 45%, white powder). The raw material EM721 was recovered 24.6 mg (Yield: 31%).  
                                                     EM738:   m. p.: 115-118° C.       IR (KBr) ν:   3430.3, 2969.8, 2933.2, 2858.0, 1712.5,           1459.8, 1378.9, 1317.1, 1263.1, 1166.7,           1126.2, 1078.0, 1047.2, 1039.4, 1016.3 cm −1 .       HRMS (FAB)m/z:   C 41 H 74 NO 12  [M + Na] +                  Calculated   772.5210           Found   772.5214.                      
 
       EXAMPLE 20  
       [0089]    Synthesis of Bis-De(3′-N-Methyl)-3′, 3′-N, N-Dihexyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM739)  
                         
 
         [0090]    N,N-Diisopropylethylamine (116.0 μL, 0.666 mmol) and 1-bromohexane (93.6 μL, 0.666 mmol) were added in this order to acetinitrile (1.1 mL) solution of EM721 (22.9 mg, 0.0333 mmol) and stirred at 60° C. for 72 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM739 (20.1 mg, Yield: 71%, white powder).  
                                                                     EM739:   m. p.: 158-160° C.           IR (KBr) ν:   3490.0, 2958.3, 2931.3, 2871.5, 2858.0,               1702.8, 1459.8, 1376.9, 1319.1, 1265.1,               1166.7, 1126.2, 1083.8, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 47 H 86 NO 12  [M + H] +                  Calculated   856.6149           Found   856.6132.                      
 
       EXAMPLE 21  
       [0091]    Synthesis of Bis-De(3′-N-Methyl)-3′-N-(2-Fluoroethyl)-8, 9-Anhydro-Pseudoerythromycin A 6, 9-hemiketal (EM740)  
                         
 
         [0092]    N,N-Diisopropylethylamine (347.7 μL, 1.996 mmol) and 1-bromo-2-fluoroethane (148.6 μL, 1.996 mmol) were added to dimethylformamide (3.3 mL) solution of EM703 (70.0 mg, 0.0998 mmol) at room temperature and stirred for 48 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM740 (36.0 mg, Yield: 48%, white powder). The raw material EM703 was recovered 25.5 mg (Yield: 36%).  
                                                                     EM740:   m. p.: 138-140° C.           IR (KBr) ν:   3480.8, 2973.7, 2937.1, 2879.2, 1704.8,               1457.9, 1376.9, 1319.1, 1265.1, 1166.7,               1126.2, 1114.7, 1076.1, 1049.1, 1035.6,               1016.3 cm −1 .           HRMS (FAB)m/z:   C 38 H 66 NO 12 Fna [M + Na] +                  Calculated   770.4467           Found   770.4469.                      
 
       EXAMPLE 22  
       [0093]    Synthesis of De(3′-N-Methyl)-3′-Cyanomethyl-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM742)  
                         
 
         [0094]    N,N-Diisopropylethylamine (320.9 μL, 1.847 mmol) and bromo acetinitrile (128.3 μL, 1.847 mmol) were added to dimethylformamide (3.1 mL) solution of EM703 (64.6 mg, 0.0921 mmol) at room temperature and stirred for 4 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM742 (53.1 mg, Yield: 78%, white powder).  
                                                                         EM742:   m. p.: 110-112° C.           IR (KBr) ν:   3485.5, 2973.7, 2935.1, 2863.8, 1702.8,               1456.0, 1382.7, 1319.1, 1265.1, 1166.7,               1126.2, 1074.2, 1037.5, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 38 H 64 N 2 O 12 Na [M + Na] +                      Calculated   763.4356               Found   763.4377.                      
 
       REFERENTIAL EXAMPLE 2  
       [0095]    Synthesis of De(12-Hydroxy)-De[12-(1-Hydroxypropyl)]-12-Oxo-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM705)  
                         
 
         [0096]    Lead tetraacetate (508.0 mg, 1.136 mmol) was added to dichloromethane (24.0 ml) solution of EM701 (508.0 mg, 0.701 mmol) and stirred at room temperature for 40 minutes. After confirming completion of the reaction by TLC, the reaction mixture was diluted with saturated brine-aqueous saturated sodium hydrogen carbonate (1:1, v/v) and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01) to obtain EM705 (282.7 mg, Yield: 61%, white powder).  
                                                                         EM705:   m. p.: 108-112° C.           IR (KBr) ν:   3488, 2972, 2883, 1740, 1724, 1458, 1379,               1244, 1165, 1107, 1093, 1076, 1055, 1034,               1016 cm −1 .           HRMS (FAB):   C 34 H 58 NO 11  [M + H] +                      Calculated   656.4010               Found   656.4021.                      
 
       EXAMPLE 23  
       [0097]    Synthesis of De(12-Hydroxy)-De[12-(1-Hydroxypropyl)]-12-Hydroxyoxime-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM743) and the Salt Thereof  
                         
 
         [0098]    Pyridine (0.9 mL) was slowly added at 0° C. to ethanol (0.9 mL) solution of EM705 (116.5 mg, 0.1781 mmol) and hydroxylamine hydrochloride (32.0 mg, 0.533 mmol) and stirred for 3 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01→10:1:0.05 ) to obtain EM743 (114.5 mg, Yield: 96%, white powder).  
                                                     EM743:   m. p.: 141-143° C.       IR (KBr) ν:   3485.8, 2971.8, 2937.1, 2883.1, 1737.5,           1459.8, 1378.9, 1255.4, 1247.7, 1166.7,           1112.7, 1089.6, 1076.1, 1037.5, 1014.4 cm −1 .       HRMS (FAB)m/z:   C 34 H 59 N 2 O 11  [M + H] +                  Calculated   671.4112           Found   671.4108.                  
 
       EXAMPLE 24  
       [0099]    Synthesis of De[(3′-N-Methyl)-[3′-N-(3-Hydroxy-1-Propyl)]-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM744)  
                         
 
         [0100]    N,N-Diisopropylethylamine (338.3 μL, 1.942 mmol) and 3-bromo-1-propanol (175.6 μL, 1.942 mmol) were added to dimethylformamide (3.3 mL) solution of EM703 (68.1 mg, 0.0971 mmol) at room temperature and stirred for 48 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM744 (27.7 mg, Yield: 38%, white powder). The raw material EM703 was recovered 22.5 mg (Yield: 33%).  
                                                                         EM744:   m. p.: 142-145° C.           IR (KBr) ν:   3478.8, 2973.7, 2937.1, 2877.3, 1700.9,               1635.3, 1459.8, 1403.9, 1382.7, 1317.1,               1267.0, 1166.7, 1126.2, 1114.7, 1076.1,               1049.1, 1035.6, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 39 H 69 NO 13 Na  [M + Na] +                      Calculated   782.4666               Found   782.4667.                      
 
       EXAMPLE 25  
       [0101]    Synthesis of De(3′-N-Methyl)-3′-N-(2-Acetoxyethyl)-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM745)  
                         
 
         [0102]    N,N-Diisopropylethylamine (106.8 μL, 0.613 mmol) and 2-bromoethylacetate (67.6 μL, 0.613 mmol) were added to dimethylformamide (1.0 mL) solution of EM703 (21.5 mg, 0.0307 mmol) at room temperature and stirred for 48 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM745 (6.0 mg, Yield: 25%, white powder).  
                                                                         EM745:   m. p.: 131-133° C.           IR (KBr) ν:   3500.2, 3477.0, 2973.7, 2937.1, 2877.3,               1735.6, 1700.9, 1457.9, 1376.9, 1319.1,               1265.1, 1166.7, 1126.2, 1078.0, 1037.5,               1016.3 cm −1 .           HRMS (FAB)m/z:   C 40 H 69 NO 14 Na  [M + Na] +                      Calculated   810.4615               Found   810.4629                      
 
       EXAMPLE 26  
       [0103]    Synthesis of De[12-(Hydroxypropyl)]-8, 9-Anhydro-Pseudo Erythromycin A 6, 9-Hemiketal (EM746)  
                         
 
         [0104]    Sodium borohydride (21.8 mg, 0,575 mmol) was added to methanol (2.9 mL) solution of EM705 (37.7 mg, 0.0575 mmol) at −78° C. and stirred for 30 minutes. Temperature of the reaction mixture was increased to 0° C. and further stirred for 30 minutes. After confirming completion of the reaction by TLC, the reaction was terminated by adding acetone (0.5 ml). The reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM746 (35.8 mg, Yield: 95%, white powder)  
                                                                         EM746:   m. p.: 116-118° C.           IR (KBr) ν:   3457.7, 2971.3, 2939.0, 1731.8, 1631.5,               1457.9, 1378.9, 1265.1, 1166.7, 1110.8,               1078.0, 1041.4, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 34 H 59 NO 11 Na [M + Na] +                      Calculated   680.3963               Found   680.3963                      
 
       EXAMPLE 27  
       [0105]    Synthesis of De(3′-Dimethylamino)-3′-Morpholino-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM747)  
                         
 
         [0106]    N,N-Diisopropylethylamine (45.8 μL, 0,263 mmol) and bis(2-bromoethyl) ether (33.1 μL, 0.263mmol) were added in this order to acetinitrile (2.6 mL) solution of EM721 (18.1 mg, 0.0263 mmol) and stirred at 80° C. for 24 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM747 (12.0 mg, Yield: 60%, white powder).  
                                                     EM747:   m. p.: 139-142° C.       IR (KBr) ν:   3452.0, 2971.8, 2937.1, 2865.7, 1700.9,           1646.9, 1457.9, 1380.8, 1319.1, 1265.1,           1166.7, 1110.8, 1072.2, 1049.1, 1016.3 cm −1 .       HRMS (FAB)m/z:   C 39 H 67 NO 13 Na [M + Na] +                  Calculated   780.4510           Found   780.4529                  
 
       EXAMPLE 28  
       [0107]    Synthesis of De(3′-Dimethylamino)-3′-[Hexahydro-1(1H)-Azepinyl]-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM748)  
                         
 
         [0108]    N,N-Diisopropylethylamine (49.5 μL, 0,284 mmol) and 1,6-dibromohexane (43.6 μL, 0.284 mmol) were added in this order to acetinitrile (2.8 ml) solution of EM721 (19.5 mg, 0.0284 mmol) and stirred at 80° C. for 24 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM748 (11.7 mg, Yield: 54%, white powder).  
                                                     EM748:   m. p.: 120-123° C.       IR (KBr) ν:   3430.7, 2971.8, 2933.2, 2858.0, 1708.6,           1629.6, 1457.9, 1378.9, 1319.1, 1263.1,           1166.7, 1112.7, 1083.8, 1047.2, 1016.3 cm −1 .       HRMS (FAB)m/z:   C 41 H 72 NO 12  [M + H] +                  Calculated   770.5054           Found   770.5062.                  
 
       EXAMPLE 29  
       [0109]    Synthesis of Bis-De(3′-N-Methyl)-3′, 3′-N, N-Di-(10-Bromo-1-Decanyl)-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM749)  
                         
 
         [0110]    N,N-Diisopropylethylamine (45.6 μL, 0,262 mmol) and 1,10-dibromodecane (58.9 μL, 0.262 mmol) were added in this order to acetinitrile (2.6 mL) solution of EM721 (18.0 mg, 0.0262 mmol) and refluxed at 80° C. for 36 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=20:1:0.1) to obtain EM749 (14.9 mg, Yield: 51%, white powder).  
                                                                         EM749:   m. p.: 132-134° C.           IR (KBr) ν:   3448.1, 2929.3, 1700.9, 1629.6, 1459.8,               1375.0, 1319.1, 1267.0, 1166.7, 1126.2,               1081.9, 1049.1, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 55 H 100 NO 12 Br 2  [M + H] +                      Calculated   1126               Found   1126.                      
 
       EXAMPLE 30  
       [0111]    Synthesis of De(12-Hydroxy)-De[12-(Hydroxypropyl)]-12-Amino-8,9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM750)  
                         
 
         [0112]    Molybdenum oxide (IV) (10.0 mg, 0,0694 mmol) and sodium borohydride (10.5 mg, 0.277 mmol) were added to ethanol (2.3 mL) solution of EM743 (15.5 mg, 0.0231 mmol) at 0° C. and stirred for 4 hours. After confirming completion of the reaction by TLC, the reaction was terminated by adding acetone (0.5 mL), and the reaction mixture was diluted with saturated brine-aqueous saturated sodium hydrogen carbonate (1:1, v/v) and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:1:0.1) to obtain EM750 (13.4 mg, Yield: 88%, white powder).  
                                                                         EM750:   m. p.: 104-107° C.           IR (KBr) ν:   3448.1, 2971.8, 2935.1, 1729.8, 1629.6,               1457.9, 1378.9, 1259.3, 1166.7, 1114.7,               1078.0, 1039.4, 1016.3 cm −1 .           HRMS (FAB)m/z:   C 34 H 60 N 2 O 10 Na [M + Na] +                      Calculated   679.4145               Found   679.4117.                      
 
       REFERENTIAL EXAMPLE 3  
       [0113]    Synthesis of De (3′-N-Methyl)-De (12-Hydroxy)-De-[12-(1-Hydroxy Propyl)]-12-Oxo-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM706)  
                         
 
         [0114]    Lead tetraacetate (508.0 mg, 1.136 mmol) was added to dichloromethane (24.0 ml) solution of EM701 (508.0 mg, 0.701 mmol) and stirred at room temperature for 40 minutes. After confirming completion of the reaction by TLC, the reaction mixture was diluted with saturated brine-aqueous saturated sodium hydrogen carbonate (1:1, v/v) and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=10:0.5:0.01) to obtain EM706 (71.6 mg, Yield: 16%, white powder).  
                                                     EM706:   m. p.: 176-179° C.       IR (KBr) ν:   3468, 2966, 2852, 2360, 1736, 1718, 1558,           1462, 1379, 1246, 1165, 1126, 1099, 1076,           1038, 1016 cm −1 .       HRMS (FAB)m/z:   C 33 H 56 NO 11  [M + H] +                  Calculated   642.3853           Found   642.3866.                      
 
       EXAMPLE 31  
       [0115]    Synthesis of De(3′-N-Methyl)-De[12-(1-Hydroxypropyl)]-8, 9-Anhydro-Pseudoerythromycin A 6, 9-Hemiketal (EM751)  
                         
 
         [0116]    Sodium borohydride (22.9 mg, 0.605 mmol) was added to methanol (3.0 mL) solution of EM706 (38.8 mg, 0.0605 mmol) at 0° C. and stirred for 1 hour. After confirming completion of the reaction by TLC, the reaction was terminated by adding acetone (0.5 mL), and the reaction mixture was diluted with saturated brine-aqueous saturated sodium hydrogen carbonate (1:1, v/v) and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM751 (31.4 mg, Yield: 81%, white powder).  
                                                                     EM751:   m. p.: 123-125° C.           IR (KBr) ν:   3504.0, 2448.1, 2971.8, 2935.1, 1729.8,               1664.3, 1594.8, 1457.9, 1378.9, 1334.1,               1265.1, 1166.7, 1126.2, 1078.0, 1041.4,               1016 cm −1 .           HRMS (FAB)m/z:   C 33 H 58 NO 11  [M + H] +                  Calculated   644.3987           Found   644.4011                      
 
       EXAMPLE 32  
       [0117]    Synthesis of De(3-O-Cladinosyl)-De(3′-N-Methyl)-8, 9-Anhydrous-Pseudoerythromycin A 6, 9-Hemiketal (EM754)  
                         
 
         [0118]    p-toluenesulfonic acid monohydrate (53.9 mg, 0.283 mmol) was added to dimethylformamide (3.8 mL) solution of EM703 (132.4 mg, 0.189 mmol) and stirred at 50° C. for 6 hours. After confirming completion of the reaction by TLC, the reaction mixture was diluted with water, adjusted to pH 8 by adding saturated aqueous sodium hydrogen carbonate and extracted with dichloromethane. The organic layer was dehydrated by adding sodium sulfate, filtered to remove the sodium sulfate, and removed the solvent to obtain crude substance. The crude substance was purified by silica gel column chromatography (chloroform:methanol:aqueous ammonia=15:1:0.1) to obtain EM754 (50.2 mg, Yield: 49%, white powder).  
                                                     EM754:   m. p.: 218-221° C.       IR (KBr) ν:   3432.7, 2969.8, 2927.4, 2858.0, 1708.6,           1629.6, 1457.9, 1405.9, 1380.8, 1319.1,           1270.9, 1232.3, 1130.1, 1078.0, 1039.4 cm −1 .       HRMS (FAB)m/z:   C 28 H 49 NO 9 Na [M + Na] +                  Calculated   566.3305           Found   566.3311.                      
 
       EFFECT OF THE INVENTION  
       [0119]    Novel pseudoerythromycin of the present invention has decreased antibacterial activity and increased antiinflammatory action, and is expected as the novel antiinflammatory agent.