Abstract:
A sterilization system preferably utilizing two baths in which articles to be sterilized are immersed into a first bath where the articles are conditioned and then into a second bath containing a sterilizing solution where they are sterilized. The ingredients for the sterilization system are stored in hermetically sealed, multicompartment pouches which are opened just prior to use to release and activate the system. The sterilization system utilizes a solution comprising 0.02 to 1% of hypochlorite, 1.4 to 20% of di(alkali metal) phosphate, 0.08% to 21% mono(alkali metal) phosphate, and 0.1 to 0.5% of nonionic surfactant with the proviso that the lower limit of di(alkali metal) phosphate is 4% when the amount of said hypochlorite is from 0.5 to 1%, said percentages being based on weight (in grams) per 100 ml. of solution. The sterilizing solution is highly efficacious while being non-corrosive and having a reduced toxicity and prolonged shelf life, and may be utilized by relatively untrained personnel.

Description:
This is a division, of application Ser. No. 924,018, filed July 12, 1978 now abandoned. 
    
    
     BACKGROUND OF THE INVENTION 
     Chlorine-based disinfectants have played an important role in medical history. As early as the 18th century, the bleaching and disinfecting properties of chlorine were recognized. In 1846, the efficacy was demonstrated of solutions prepared from chloride of lime for prevention of puerperal fever. Shortly thereafter, calcium hypochlorite was used for treating water. World War I prompted investigation of the use of chlorine solutions for cleansing infected wounds. In 1915, Dakin reported experiments concerning the chlorine solution which bears his name (Dakin, H. D.: The antiseptic action of hypochlorites, Brit. Med. J. ii: 809 Dec. 1915). Subsequently, the results of lavaging foul wounds with Dakin&#39;s solution were lauded. 
     Bacteriological studies in this century investigated vital parameters of chlorine solutions. Germicidal action depends on the concentration of hypochlorous acid (Charlton, D. &amp; M. Levine: Germicidal properties of chlorine compounds, Iowa State College Bulletin 35:48, 1937). Both the quantity of chlorine compounds and the pH of the solution determine the concentration of hypochlorous acid. The higher the pH of a chlorinated water solution, the lower the concentration of undissociated hypochlorous acid. At pH 7.5 approximately 50% of the chlorine concentration will exist as undissociated hypochlorous acid (HOCl) while the other 50% will be present as hypochlorite ion (OCl) (White, G.C.: Handbook of Chlorination, N.Y., Van Nostrand Reinhold, 186, 1972). Maximum efficacy of a given concentration of chlorine in solution occurs below pH 5, when all of the chlorine present exists as undissociated hypochlorous acid. Bacteriological tests substantiate that, as hypochlorite solutions are acidified, a marked increase in germicidal action occurs. With pH control, chlorine has proved to be one of the most potent and reliable germicides. As such, it became widely used in water and sewage treatment. 
     Early studies elucidated a number of problems with chlorine distinfectants. Dakin (previously identified) recognized the &#34;highly irritating character&#34; of some chlorine solutions and learned that tissue irritation could be reduced if both the concentration of hypochlorites and the pH were controlled. Dakin discussed the importance of neutralizing the solution by adding acid to the alkaline hypochlorite compound. Dakin&#39;s &#34;neutralized&#34; solution could be continuously applied to wounds without serious irritation. 
     Another problem is that chlorine solutions are very unstable. The effectiveness of these solutions deteriorates significantly within a few hours. This problem depends largely on the pH of the solution. The more alkaline a hypochlorite solution is, the less hypochlorous acid is present, the less effective it is as a sterilant, and the more stable it is. An example of such an extremely stable hypochlorite solution is ordinary household bleach. The more acid such a hypochlorite solution is, the greater is its hypochlorous acid content, the more effective it is as a sterilant, and the greater the instability of the solution. Cullen indicated that the lower limit of pH of Dakin&#39;s solution was about 9, for below that level the solution became too unstable for clinical use (Cullen, G. E. &amp; H. D. Taylor: Relative irritant properties of the chlorine groups of antiseptics, J. Exp. Med. 28:681, 1918). Depending on the initial concentration of hypochlorite, the effective halflife of an acid solution may be only a few hours. A truly neutralized chlorine solution must be mixed immediately prior to use. 
     The high reactivity of chlorine creates another major problem with its use as a sterilant. Early in this century, Carrel recognized a substantial reduction in the germicidal efficacy of chlorine solutions to which blood serum had been added and remarked about the rapid disappearance of hypochlorite in contact with body tissues and fluids (Carrel, A. &amp; G. Dehelly: The treatment of infected wounds, London, Univ. of London Press, 1918). In a more recent study, addition of only 300 ppm of lactose, a natural sugar, was found to significantly reduce the efficacy of a chlorine solution (Rudolf, A. S. &amp; M. Levine: Factors affecting the germicidal efficacy of hypochlorite solutions, Iowa State College Bulletin, 40:35, 1941). Chlorine readily combines with a wide variety of organic and inorganic substances and is thereby inactivated. Reliable use of a chlorine-based germicide requires adequate cleansing of materials to be sterilized to remove such ubiquitous contaminants as protein or grease. That cleansing must be performed with compatible agents. Most common cleansing agents are not compatible because they bind and inactivate chlorine compounds. 
     Chlorine solutions have no inherent wetting or detergent capacity. Therefore, microorganisms which are encased in an air bubble or in an oil drop escape destruction. Addition of a surface active or wetting agent to the chlorine solution can increase penetration and contact. Careful evaluation is required to assure that the agent used is compatible and does not bind and inactivate significant amounts of chlorine. Addition of specific compatible detergents to chlorine solutions has been reported to increase their germicidal efficacy. (Petroff, S. A. &amp; P. Schain: The enhancement of bactericidal properties of well known antiseptics by addition of detergents, Quart. B. Seaview Hosp. 5:378-379, 1940). Such combinations of chlorine compounds with compatible surfactants, though fine germicides, are not now generally used as sterilants because of their corrosive character. 
     The most difficult to solve problem of chlorine-based germicides is that they are highly corrosive. Concentrations of hypochlorous acid sufficient to sterilize standard bacteriological challenges also quickly attack metals, even stainless steel, causing discoloration and pitting. Metal instruments soaked in chlorine solutions tend to be irreversibly damaged. Sharp edges are destroyed and metal surfaces become pitted and darkened. Instrument manufacturers have been known to remark that they dislike having a product exposed to a chemical which magnifies any metallurgical flaw in even the best stainless steel. Corrosion inhibitors have been recommended for use with hypochlorites (Botham, G. H. &amp; G. A. Dummett: Corrosion by commercial sodium hypochlorite and its inhibition, J. Dairy Res. 16:37, 1949). Sodium silicate was found to be effective in an alkaline solution containing 150 ppm available chlorine. The increase of alkalinity, however, substantially decreases germicidal efficacy. Sodium silicate insufficiently retards corrosion in more concentrated hypochlorite solutions. Many other anticorrosive agents are incompatible because they react with and inactivate chlorine. Resolution of this problem is required in order to provide a chlorine sterilant which is safe for instruments. 
     Dr. J. C. Kelsey investigated liquid disinfectants for many years. He developed the &#34;Kelsey-Sykes&#34; test, which is now the British referee bacteriological challenge test. In recent years, Dr. Kelsey recommended sodium hypochlorite solutions as the most efficacious liquid disinfectants. He tried to develop an optimal chlorine solution and published a report of his work in 1974 (J. Clin. Path. 27, 632-638). The chlorine solution which he recommends in that article is highly toxic because of its methanol content and its unadjusted alkaline pH. It is also extremely corrosive. 
     Since Dr. Kelsey retired, Dr. David Coates has carried on the work on hypochlorite solutions. He published a report of his work in 1978 (J. Clin. Path. 31, 148-152). Further experiments indicated that the hypochlorite solutions which he had reported in that publication, were too corrosive for routine use. Therefore, to control the corrosive and tissue-irritating properties of the solution, Dr. Coates reduced the concentrations of alcohol and of available chlorine (to about 200 ppm). He has also learned that buffering the pH of the solution is vital. The chlorine solution which he has most recently recommended, however, contains neither a satisfactory surfactant nor an anticorrosive agent. 
     Despite the long history of efficacious use of chlorine compounds as disinfectants, few chlorine compounds today are used as sterilants in medical practice. None of the existing solutions meets the requirements of an optimal chlorine-based sterilizing system. The ideal characteristics of such a system are the following: 
     1. It must reliably kill the standard U.S. Government bacteriological challenge (called the &#34;AOAC&#34; test), preferably within a contact time of 30 minutes or less. Existing liquid sterilants require contact times of many hours to pass the &#34;AOAC&#34; test. 
     2. It must be completely non-corrosive and non-damaging within the recommended contact time. 
     3. Its pH must be adjusted to and maintained at approximately neutral in order to increase efficacy, reduce toxicity, and avoid the rapid deterioration of potency which occurs in more highly acid chlorine solutions. 
     4. It must assure proper preparation of materials to be sterilized, in a way which avoids contamination by organic material or chemicals which might inactivate the chlorine. 
     5. The system must be so simple that even untrained personnel can prepare and use it without significant error. 
     In order to maintain the pH of a solution within a desired range, buffers are usually used. One of the most common buffers in the neutral range contains phosphates. For buffering purposes, less than 1% of phosphates will suffice. A solution containing 0.2% hypochlorite, buffered at pH 7.5 with phosphates, is an effiacious sterilant. Such a solution, however, is corrosive and has undesirable surface tension characteristics. 
     Many investigators have used standard concentrations of phosphates to buffer chlorine solutions (e.g. Friberg, L. &amp; E. Hammarstrom: The action of available chlorine on bacteria and bacterial viruses, Acta. Pathl. Microbiol. Scand., 38:128, 1956). Phosphates are included in lists of corrosion inhibitors (Uhlig, H. H: The Corrosion Handbook, NY, Wiley &amp; Sons, 906-7 and 913-14, 1948). Concentrations of phosphates under 1% have been added to chlorine-based disinfectants to reduce corrosion (Diversol EX, Brit. Pat. No. 781,708). However, it has not heretofore been known to use concentrations of phosphates over 1% to achieve substantial corrosion control. 
     According to the present invention, it has been determined that concentrations of phosphates in excess of 1% markedly reduce the corrosiveness of the solution. The concentration of phosphates necessary to control corrosion depends on several factors including the concentration of hypochlorite, the pH, the presence of a surface active agent, the contact time, and the type of steel. In a solution at pH 7.5 containing 0.02% sodium hypochlorite and a compatible surface active agent, addition of a total of 1.17% phosphates produces a 93% reduction in the corrosion of carbon steel during 4 hours contact. In a similar solution containing 0.2% sodium hypochlorite, addition of a total of 2.75% phosphates produced a 97% reduction in the corrosion of carbon steel during 2 hours contact. When the concentration of total phosphates in the latter solution was increased to approximately 7%, no notable corrosion of common steel occurred. In this instance, even 24 hours of soaking of common steel produced no visible change. 
     The combination of phosphates in the solution acts as a buffer, resisting alteration in the pH. The concentrations of acid and alkaline phosphates are adjusted to produce approximate neutrality. Under this circumstance, a solution containing as high a hypochlorite concentration as 0.2% exhibits little tendency to tissue irritation. When that solution is repeatedly dropped into the eyes of rabbits, according to directions for the Draize test, no evidence of inflammation occurs (see Example IX). 
     According to the present invention a surface active agent is included in the chlorine solution. Air bubbles on material not only prevent sterilization but also promote corrosion. The addition of a detergent reduces surface tension and helps avoid formation of bubbles. Few detergents, however, are compatible with both chlorine and phosphates and also effective at pH 7.5. Some effective surfactants which do not react with chlorine, for example, combine with the phosphates causing precipitation. A nonionic agent which meets the desired requirements is a 12 carbon alkyldimethyl amine oxide, for example, dimethyl lauryl amine oxide (see U.S. Pat. No. 3,296,145). This amine oxide is available under the following trademarks: Ammonyx LO and Barlox 12. 
     The addition of this amine oxide to the sterilizing system of the present invention increases the efficacy of the solution. Suture loops placed on the surface of a buffered chlorine solution lacking detergent will float. If the loops are forced under the surface, many tiny bubbles are visible on their surfaces. When a solution contains an adequate surfactant, the loops rapidly sink into the solution without visible bubbles. With the addition of the amine oxide, solutions of the present invention exhibit adequate surface tension characteristics (see Example VIII). 
     Inclusion of the surfactant in the sterilizing system of the present invention also measurably enhances corrosion inhibition. For example, when approximately 5% phosphates are added to a solution containing 0.2% hypochlorite at pH 7.5, an 85% reduction in corrosion occurs. When dimethyl lauryl amine oxide is then added to that combination, corrosion virtually ceases. Some surface active agents are listed among corrosion inhibitors (Uhlig, H. H.: The Corrosion Handbook, N.Y., Wiley &amp; Sons, 910-911). However, no previous report is known of potentiation of corrosion inhibition by a combination of phosphates and a surface active spent added to a chlorine-based sterilizing system. 
     In order to avoid inactivation of the germicidal solution by contaminants such as inorganic salts, organic material or incompatible detergents, and assure thorough wetting of the instrument to be sterilized, immersion in a conditioning bath may be added to the sterilizing system. The conditioning bath consists of ample quantities of separate washing composition containing the same amine oxide as well as agents to control corrosion and pH. Thus, all materials to be sterilized would be preconditioned in a solution containing the conditioning composition. Transfer of small amounts of the conditioning solution to the sterilizing solution will not counteract the efficacy of the latter. 
     Shelf-life requirements necessitate that the major components of the sterilizing solution (hypochlorite, phosphates and surfactant) remain separate until mixed for use. To avoid errors in preparation, a compartmented bag was devised which assures complete mixing of all compounds when the bat is opened. A colored indicator added to the component in the center compartment acts as a safeguard. Alteration in the central color of the package warns the user that leakage between compartments has occurred. The indicator color immediately disappears when combined with small quantities of hypochlorite. To complete the sterilizing solution, the three compartments in he package are added to a measured amount of water. 
     Bacteriological tests on a specific formulation of the sterilization system, Solution O (see Examples V-VII) prove it to be a remarkable germicide. High titres of vegetative organisms are killed within minutes. The solution readily passed Great Britain&#39;s referee challenge, the &#34;Kelsey-Sykes&#34; test (Kelsey, J. C. &amp; I. M. Maurer: An improved (1974) Kelsey-Sykes test for disinfectants. Pharm. J., Nov. 30, 1974). The sterilization system of the present invention can reliably pass within 30 minutes the American &#34;AOAC Sporicidal Test&#34; which often represents the most difficult bacteriological hurdle. Although some decay in available chlorine occurs during the 24 hours useful life of the solution, that change is insufficient to affect germicidal potency. A 24-hour old solution also passes the &#34;AOAC Sporicidal Test&#34;. 
     Accordingly, an object of the present invention is to provide a sterilization system which is efficacious as a sterilant, which is non-corrosive and non-damaging, which has no deleterious effects on instruments within the recommended contact time, has little tissue irritating properties, has good wetting capacity, and which is stable for more than twenty-four hours. 
     Another object is to provide a reliable liquid sterilant system operable within a short enough cycle for practical application. 
     Another object is to provide a liquid sterilant of increased efficacy, reduced toxicity and prolonged life in which the potency does not rapidly deteriorate. 
     Another object is to provide a sterilization system which does not become contaminated by the materials being sterilized. 
     A further object is to provide a sterilization system which is so simple that even untrained personnel can prepare and use it without significant chance of error. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 is a schematic view illustrating one embodiment of the sterilization system of the present invention. 
     FIG. 2 is an elevational view of the tri-pouch used in the sterilization system of the present invention. 
     FIG. 3 is a sectional view taken along the lines 3--3 in FIG. 2. 
     FIG. 4 is a sectional view similar to FIG. 3 of an alternate arrangement. 
     FIG. 5 is a sectional view similar to FIG. 3 of a further alternate arrangement. 
    
    
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     Referring to the drawings, FIG. 1 shows an overall view of one embodiment of the sterilizing system of the present invention. The illustrated sterilization system involves the use of two sealed plastic pouches 10 and 12. The entire contents of each pouch 10, 12 are mixed with water in the containers 14, 16 respectively and the contents of the containers 14, 16 are then emptied into wide mouth containers or pans 18, 20 respectively which define two baths in which the sterilization process is performed as will be described. 
     In the illustrated embodiment, FIG. 2, the contents of the first pouch (10) used for the first bath contains cleaning and conditioning agents. The second pouch (12) for the second bath is a tri-pouch in that it consists of three separate, sealed compartments 22, 24, 26 in which one outer compartment (22 or 26) contains a hypochlorite solution, the other outer compartment (22 or 26) contains a buffer and anticorrosive agent, and the intermediate compartment (24) sandwiched there between contains a surfactant or wetting agent preferably with a color indicator. 
     Turning to details of the illustrated embodiment, the tri-pouch (12) may be made from four sheets of plastic film (31, 32, 33, 34,) which may be superimposed one on top of the other and heat sealed together at their edges (36) to form a pouch having the three separate compartments (22, 24, 26). Each of the three separate compartments (22, 24, 26) is completely sealed from one another such that the three liquid components will be kept separate until the moment of use of the sterilization system. 
     The sheets of plastic film (31-34) of the tri-pouch (12) may each be made of a double thickness film as illustrated at 31a, 31b, 34a, 34b respectively in FIG. 3 to provide added integrity to prevent leakage through any pin holes which may occur during manufacture of single thickness plastic film. The term double thickness may refer to what is commercially known as co-extruded or laminated film. 
     The tri-pouch (12) has a portion with converging side seams to form a tapered or funnel-like end portion (38) as shown in the drawings. To release the three liquid components from the tri-pouch (12), it is only necessary to snip off the pointed end of the tri-pouch (as indicated at 40) with a pair of scissors or the like to simultaneously cut through all three compartments. Accordingly, the liquid contents of all three compartments (22, 24, 26) of the tri-pouch will pour out simultaneously, thereby mixing the three components and activating the sterilization system. The contents of the tri-pouch (12) are emptied into the previously mentioned container (16) which contains water or to which water is added. Thereafter the contents of the container (16) is emptied into the pan (20) forming the second bath. The use of the tri-pouch (12) permits storage of the correct and premeasured amounts of the separated liquids until ready for use. 
     As previously indicated, when it is desired to use the sterilization system, the tapered end (34) of the tri-pouch (12) is merely snipped off and the three liquid ingredients will pour out simultaneously to mix during the pouring operation. Thus, the use of the three compartment pouch greatly reduces the chance of a partial mixing and virtually prevents use of less than all of all three of the components of the tri-pouch. 
     The arrangement of the tri-pouch also has the advantage that if either the liquid sterilant or the buffer should leak internally, they would leak into center compartment (24) containing the wetting agent and the color indicator to effect a color change and thereby provide a visual indication that a leak has occurred. By way of example, the color indicator may be chlorophenol red solution which gives the center compartment (24) a reddish purpole color. If the liquid sterilant solution (e.g. hypochlorite) in one of the outer compartments, 22 or 26, were to leak into the center compartment (24), the chlorine/hypochlorite would oxidize the reddish purple color indicator and the package would appear as a pale straw yellow of the hypochlorite solution. Conversely, should the buffer and anticorrosive solution (e.g. phosphate) in the other of the outer compartments, 22 or 26, leak internally, it would cause a precipitate to occur in the center compartment (24). Thus, the absence of the reddish purple color or the presence of the precipitate would be an indication that an internal leak has occurred. The color indicator is thereby able to provide a visual determination of whether or not an internal leak has occurred at any time between the filling of the tri-pouch (12) to the time it is ready for use. Accordingly, assurance is readily provided to the user that no internal leakage has occurred and that the contents of the tri-pouch are safe to use. 
     The walls (31-34) of the tri-pouch (12) are made of a clear plastic film so that the contents can be readily observed. By way of example, the plastic film may be polyethylene, polyethylene-ionomer coextrusion, polyethylenepolyamide laminate, or the like and may have a thickness of from 0.002 in. to 0.020 in., preferably 0.006 in. 
     As an alternate arrangement, the membranes or septa of the tri-pouch could be of a double thickness such as indicated, for example, at 31c and 31d in FIG. 5, with various color indicators interposed between the two thicknesses as indicated at 41, to provide further and easily detected indication of a leak in one of the inner septa. 
     The pouch (10) containing the cleansing and conditioning agents may also be made of a clear plastic film and heat sealed around the edges (44). By way of example, the plastic film may be polyethylene, polyethyleneionomer coextrusion, polyethylene-polyamide laminate, or the like and may have a thickness of from 0.002 in. to 0.020 in., preferably 0.006 in. 
     Returning to FIG. 1, when it is desired to sterilize, the first pouch (10), containing the cleansing and conditioning agent, is opened and emptied into the container (14). Tap water is then added to the container (14) to bring the mixed contents to a given quantity of solution as may be indicated by marks on the container (14). The resulting diluent is then poured into the pan (18) to provide a bath in which the various items to be sterilized may be conditioned prior to immersion in the sterilizing solution. 
     Similarly, the tri-pouch (12) is snipped off at 40 by a pair of scissors as previously described and all three liquid components simultaneously emptied into the container 16. As with the first pouch (10), the container (16) is then filled with clear tap water to a mark which may be provided on the container (16) and the resulting diluent subsequently poured into the second bath (20). 
     The items to be sterilized (44) are first immersed into the first pan (18) wherein such items are conditioned and then immersed into the second pan (20) where they are sterilized. 
     The purpose of the first bath containing the cleansing and conditioning agents is to clean the instruments to be sterilized by removing such ubiquitous organic contaminants as proteins or grease, to dilute and neutralize inorganic contaminants which otherwise might react with the sterilant, and to thoroughly wet the surfaces of the item to be sterilized. This conditioning solution is utilized because the chlorine in the second bath readily combines with a wide variety of orgaic and inorganic substances and is thereby inactivated. Thus, the conditioning in the first bath (10) is performed with a cleaning agent which is compatible with chlorine, a 12 carbon alkyldimethyl amine oxide, for example, dimethyl lauryl amine oxide, which may be obtained, for example, from Onyx Chemical Company, Division of Millmaster Onyx Corp. of Jersey City, N.J., under the name of &#34;Ammonyx LO&#34;, and which is set forth in U.S. Pat. No. 3,296,145. Also contained in the first bath are potassium pyrophosphate and tripotassium phosphate which serve to saponify oily substances, sequester metallic ions, deflocculate particles, act as anticorrosive agents, and aid in the pH control of the solution. Pylam turquoise blue acts as a coloring agent and may be omitted or another coloring agent may be substituted. 
     An example of a specific composition in the first pouch (10) is: 
     7.5 g. potassium pyrophosphate 
     7.5 g. tripotassium phosphate 
     38 ml. dimethyl lauryl amine oxide (30% active) 
     0.0128 g. Pylam turquoise blue 
     60 ml. tap water 
     The first bath is readily prepared by mixing the contents of first pouch (10) with 3.3 liters of tap water to form 3.4 liters of solution. If the amounts are doubled, 6.8 liters of solution are obtained, etc. 
     The second aqueous bgath in the pan (20) desirably comprises: 
     0.02 to 1% of an alkali metal or alkaline/earth metal hypochlorite 
     0.1 to 0.5% of a nonionic surfactant which is compatible with chlorine and phosphates (30% active) 
     1.4 to 20% of di (alkali metal) phosphate 
     titrated to a pH of 7.0 to 8.0 with mono (alkali metal) phosphate, with the proviso that the lower limit of di (alkali metal) phosphate is 4.0% when the amount of said hypochlorite is from 0.5 to 1%, these percentages being based on the weight of the ingredient (in grams) per 100 ml of solution. The amount of mono (alkali metal) phosphate required to obtain the indicated pH ranges from 0.08 to 21%. The use of 0.05 to 0.3% of sodium hypochlorite, 0.2 to 0.4% of dimethyl lauryl amine oxide (30% active), 2 to 13% of dipotassium phosphate, titrated with monopotassium phosphate to a pH from 7.2 to 7.7 are preferred. The amount of monopotassium phosphate required to obtain the indicated pH ranges from 0.33 to 9.66%. 
     As an example, to obtain the aforementioned second bath, one of the outer compartments, say outer compartment 22, of the tri-pouch suitably contains 136 ml of 6% sodium hypochlorite. 
     The other outer compartment (26) suitably has the following contents: 
     314 ml. water 
     360 g. dipotassium phosphate 
     80 g. monopotassium phosphate 
     0.12 g. sodium chromate 
     The chromate is used as a colorant and can be replaced by other colorants which do not react adversely with the chlorine. The chromate can alternatively be added to the hypochlorite compartment or can be omitted. 
     The center compartment (24) of the tri-pouch (12) suitably has the following contents: 
     14.1 ml. dimethyl lauryl amine oxide (30% active) 
     5.2 ml 0.04% chlorophenol red solution 
     80.7 ml. water 
     If the color of the chlorophenol red disappears, this means that the solution from at least one of the outer compartments has leaked into the center compartment (24). In this case, the affected tri-pouch should be discarded. Colorants other than chlorophenol red can be used so long as they do not adversely affect the final chlorine bath. 
     The second bath is readily prepared by mixing the contents of the sealed compartments of the tri-pouch (12) with 3.3 liters of water to form 4 liters of solution. If the amounts are doubled, 8 liters of solution are obtained, etc. 
     The recommended concentration of 0.2% hypochlorite provides an extremely generous safety margin. The solution is still fully effective when it is 24 hours old though it contains about one-third less available chlorine. Generally, the solution will be effective for 48 to 72 hours. 
     The pH of the second bath preferably is 7.5 to provide minimum tissue irritancy, although it may vary from 7.0 to 8.0. In addition to controlling pH, the phosphates act as saponifiers and cleaning agents. 
     In operating the invention, the instruments to be sterilized may be immersed into the first bath (18) for a period on the order of 2 minutes and then are transferred to the second bath where sterilization is affected in 15-30 minutes. As previously indicated, transfer of small amounts of the cleansing solution from the first bath to the second bath will not counteract the efficacy of the sterilant. 
     As an indication of the uniqueness of a 12 carbon alkydimethyl amine oxide as a surfactant in the sterilizing compositions of this invention, the following surfactants were found to be incompatible with sodium hypochlorite or hypochlorous acid: 
     
         ______________________________________      IONICTRADEMARK  TYPE      DESCRIPTION______________________________________Aerosol OS anionic   sodium isopropyl napthalene                sulfonateAdvawet #33      nonionic  fatty acid ester glycolAdvawet #43      nonionic  fatty acid ester glycolAmmonyx CDO      *nonionic coco amido propyl dimethyl                amine oxideAvirol 300 anionic   triethanolamine alkyl sulfateAvirol 116-E      anionic   sodium lauryl ether sulfateChemadene 300      amphoteric                amine saltCerfak 1400      anionic   alkyl polyoxyethylene etherCordon 300 anionic   sulfated oilDetersol             pine oil detergentT-SpecialEmkapon TS anionic   amide sulfonateEmkatex AA anionic   alkyl aryl sulfonateFoamole AR nonionic  coconut oil alkanol amideGafac RA-600      anionic   free acid of complex                organic phosphate esterKlearfac AA-250      anionic   free acid of organic                phosphate esterLonzaine 12-C      amphoteric                coco alkyl amino betaineMiranol JEM      amphoteric                dicarboxylic octoic sulfonated                amineMiranol JS amphoteric                carboxylated sulfonated amine                of octoic acidMaprofix 60-S      anionic   sodium lauryl ether sulfateMerpol HC  nonionic  ETO condensate of long chain of                fatty alcoholMonoflor 52      nonionic  fluorohydrocarbonNopcowet 50      anionic   sulfonated alkyl esterOlapon ND-100      nonionic  polyethylene glycol ether                of linear alcoholPleurafax A-38      nonionic  straight chain aliphatic oxy-                alkylated alcoholPleuronic L-64      nonionic  ethylene oxide condensateRichonate TAB      anionic   triethanolamine dodecyl benzene                sulfonateRichonate 45-H      anionic   sodium dodecyl benzene                sulfonateRueterg 97-S      anionic   alkyl aryl sulfonateSellogen   anionic   methyl taurine condensateconcentrateSiponic L-4      nonionic  ethoxylated lauryl alcoholStandapol AB-45      amphoteric                alkyldimethyl betaineStandapol ES-3      anionic   sodium lauryl ether                (3 moles ETO) sulfateStandapol CLB-50      amphoteric                betaineStandamox 01      nonionic  oleyl dimethyl amine oxideTamol SN   anionic   sodium napthalene sulfonateTriton X-114      nonionic  octyl phenoxypolyethoxyethanolTween 20   nonionic  polyoxyethylene (20) sorbitan                monolaurateTween 80   nonionic  polyoxyethylene (20) sorbitan                monolaurate sodium                amine phosphateWayhib S   anionic   sodium amine phosphateZonyl FSN  nonionic  fluorocarbon complexZonyl FSA  anionic   fluorohydrocarbon complex                All cationic surfactants______________________________________ *nonionic above pH 7, cationic below ph 7 
    
     The following surfactants were found to be compatible with hypochlorite solutions but incompatible with the concentration of phosphate in test Solution Q (see Example V): 
     
         ______________________________________      IONICTRADEMARK  TYPE      DESCRIPTION______________________________________Abex 18-S  anionicAmmonvx MO nonionic*Ammonyx MCO      nonionic*Tetranol   anionic   sulfated fatty esterGemtex HC-70      anionic   sodium dihexyl sulfosuccinateStandapol ES-2      anionic   sodium lauryl ether                (2 moles ETO) sulfateSipon LSB  anionic   sodium lauryl sulfateMersonol 840      anionic   sodium dihexyl sulfosuccinateAerosol CT-100      anionic   sodium dioctyl sulfosuccinateStandapol WAQ      anionic   sodium lauryl sulfateTriton X-301      anionic   sodium alkyl aryl polyether                sulfateCarbanone BD      anionic   carboxylated polyethoxy of                linear alcoholMonoflor-31      anionic   fluorohydrocarbon complexAerosol CTTriton X-200      anionic   sodium alkyl aryl polyether                sulfonateSiponic E-2      nonionic  ethoxylated (2 moles ETO) cetyl                stearyl alcoholSteol CS-46      anionic   sodium coconut ether sulfatePetrowet R anionic   sodium hydrocarbon sulfonateAlkanol 189-S      anionic   sodium hydrocarbon sulfonateVictawet 35-B      anionic   polyphosphate of aliphatic groupVictawet 58-B      anionic   phosphorated higher alcohol**______________________________________ *nonionic above pH 7 **cloudy when sodium hypochlorite is added 
    
     The uniqueness of phosphates as corrosion inhibitors for use in hypochlorite solutions is indicated by the following investigative results. The following corrosion inhibitors do not completely inhibit the corrosion caused by hypochlorite solutions. 
     1. Sodium silicate is an alkaline salt. It raises the pH of the chlorine solutions to 10.5. Lowering the pH causes the formation of insoluble silicic acid, which is a very weak acid having a dissociation constant of 2×10 -10 . Sodium silicate inhibits corrosion in its alkaline environment (Botham, G. H. &amp; G. A. Dummet, Corrosion by commercial sodium hypochlorites and its inhibition, J. Dairy Res., 16:23, 1949) but the undissociated silicic acid has no corrosion inhibiting properties because of its low solubility. 
     2. Sodium molybdate was found to reduce corrosion of sodium hypochlorite solutions at a pH of 7.5 to 8, but it did not completely eliminate corrosion when tested up to a concentration of 10%. Sodium molybdate does not bind free chlorine. 
     3. Zinc ions form insoluble zinc hydroxide at pH 7.5 and, therefore, are of no use as a corrosion inhibitor for use in hypochlorite solutions. The dissociation constant for zinc hydroxide is 9.6×10 -4  and it is insoluble in water. 
     4. Sodium chromate was found to reduce corrosion of sodium hypochlorite solutions at a pH of 7.5 to 8, but did not completely eliminate corrosion when tested up to a concentration of 1%. Sodium chromate is toxic in high concentrations. 
     5. Sodium sulfite is a reducing agent and will reduce Cl+ to Cl-. 
     6. Sodium nitrite is a reducing agent and will reduce Cl+ to Cl-. 
     7. The surfactant, Chemadene 300, was found to eliminate corrosion when used in high concentrations, but it was also found to bind free chlorine. 
     The following are exemplifications of various aspects of this invention. 
     EXAMPLE I 
     Controlling Corrosion of Metals in Sodium Hypochlorite Solutions 
     PURPOSE: 
     The purpose of this experiment is to demonstrate the corrosion inhibiting effect of increasing concentrations of mono and dipotassium phosphate in sodium hypochlorite solutions, with and without a nonionic surface active agent. 
     MATERIALS: 
     1. Sodium hypochlorite (Purex Bleach) 
     2. Monopotassium phosphate (KH 2  PO 4 ) 
     3. Dipotassium phosphate (K 2  HPO 4 ) 
     4. Dimethyl lauryl amine oxide (30% active) 
     5. Disposable carbon steel scalpel blades, #11 (Fisher Scientific Cat. #9-916-B) 
     TEST SOLUTIONS: 
     A. 0.2% NaOCl--(pH 10.5) 
     B. 0.2% NaOCl, 0.35% amine oxide--(pH 10.5) 
     C. 0.2% NaOCl, 0.24% KH 2  PO 4  --(pH 7.5) 
     D. 0.2% NaOCl, 0.24% KH 2  PO 4 , 0.35% amine oxide--(pH 7.5) 
     E. 0.2% NaOCl, 0.43% KH 2  PO 4 , 0.5% K 2  HPO 4  --(pH 7.5) 
     F. 0.2% NaOCl, 0.43% KH 2  PO 4 , 0.5% K 2  HPO 4 , 0.35% amine oxide--(pH 7.5) 
     G. 0.2% NaOCl, 0.55% KH 2  PO 4 , 1% K 2  HPO 4  --(pH 7.5) 
     H. 0.2% NaOCl, 0.55% KH 2  PO 4 , 1% K 2  HPO 4 , 0.35% amine oxide--(pH 7.5) 
     I. 0.2% NaOCl, 0.75% KH 2  PO 4 , 2% K 2  HPO 4  --(pH 7.5) 
     J. 0.2% NaOCl, 0.75% KH 2  PO 4 , 2% K 2  HPO 4 , 0.35% amine oxide--(pH 7.5) 
     K. 0.2% NaOCl, 1.02% KH 2  PO 4 , 4% K 2  HPO 4  --(pH 7.5) 
     L. 0.2% NaOCl, 1.02% KH 2  PO 4 , 4% K 2  HPO 4 , 0.35% amine oxide--(pH 7.5) 
     M. 0.2% NaOCl, 1.18% KH 2  PO 4 , 6% K 2  HPO 4  --(pH 7.5) 
     N. 0.2% NaOCl, 1.28% KH 2  PO 4 , 6% K 2  HPO 4 , 0.35% amine oxide--(pH 7.5) 
     O. 0.2% NaOCl, 1.35% KH 2  PO 4 , 8% K 2  HPO 4  --(pH 7.5) 
     P. 0.2% NaOCl, 1.35% KH 2  PO 4 , 8% K 2  HPO 4 , 0.35% amine oxide--(pH 7.5) 
     PROCEDURE 
     Sixteen pairs of #11 surgical disposable carbon steel scalpel blades were accurately weighed on a micro balance. Each pair of blades was put into a separate test solution and the blades were allowed to soak for two hours. At the end of the soaking period, any corrosion products formed on the blades were washed off. The blades were then room air dried for one hour and each pair was reweighed on the micro balance. The weight loss for each pair of blades was computed. 
     
                       TABLE NO. 1______________________________________RESULTSTESTSOLU-  INITIAL   FINAL     WEIGHT  % WEIGHTTIONS  WEIGHT    WEIGHT    LOSS    LOSS______________________________________A      732.32 mg.            728.50 mg.                      3.82 mg.                              0.522%B      740.49 mg.            737.31 mg.                      3.18 mg.                              0.429%C      755.21 mg.            752.24 mg.                      2.97 mg.                              0.393%D      758.10 mg.            756.12 mg.                      1.98 mg.                              0.261%E      753.37 mg.            751.74 mg.                      1.63 mg.                              0.216%F      750.60 mg.            749.30 mg.                      1.30 mg.                              0.173%G      735.15 mg.            731.70 mg.                      3.45 mg.                              0.469%H      740.09 mg.            738.12 mg.                      1.97 mg.                              0.266%I      727.00 mg.            726.42 mg.                      0.58 mg.                              0.080%J      753.91 mg.            753.81 mg.                      0.10 mg.                              0.013%K      742.96 mg.            742.18 mg.                      0.78 mg.                              0.105%L      750.93 mg.            750.92 mg.                      0.01 mg.                              0.001%M      753.15 mg.            753.14 mg.                      0.01 mg.                              0.001%N      752.18 mg.            752.18 mg.                      0.00 mg.                              0.000%O      755.31 mg.            755.30 mg.                      0.01 mg.                              0.001%P      735.65 mg.            735.65 mg.                      0.00 mg.                              0.000%______________________________________ 
    
     
                       TABLE NO. 2______________________________________                 %TEST     SCALPEL      CORROSION INHIBITIONSOLUTION WEIGHT LOSS  FROM &#34;A&#34;______________________________________A        3.82 mg.     --B        3.18 mg.     16.8%C        2.97 mg.     22.3%D        1.98 mg.     48.2%E        1.63 mg.     57.3%F        1.30 mg.     66.0%G        3.45 mg.      9.7%H        1.97 mg.     48.4%I        0.58 mg.     84.8%J        0.10 mg.     97.4%K        0.78 mg.     79.6%L        0.01 mg.     99.7%M        0.01 mg.     99.7%N        0.00 mg.     100.0%O        0.01 mg.     99.7%P        0.00 mg.     100.0%______________________________________ 
    
     CONCLUSION 
     A solution of 0.2% sodium hypochlorite in water was very corrosive to the steel scalpel blades. The addition of the amine oxide to the hypochlorite reduced the corrosion by 16.8%. 
     Buffering the sodium hypochlorite solution to pH 7.5 with monopotassium phosphate reduced the corrosion by 22.3%. The addition of amine oxide further reduced the corrosion of the buffered hypochlorite by 33.3%. The amine oxide, therefore, reduced the corrosion twice as much in the buffered hypochlorite than it did in the unbuffered hypochlorite. 
     The addition of increasing amounts of dipotassium phosphate to the sodium hypochlorite solution, and buffering the solution to a pH of 7.5 with monopotassium phosphate, showed increasing corrosion inhibition (see Table No. 2). The addition of amine oxide showed greatly increasing inhibition with increasing concentrations of phosphate. Only the combination of phosphates and amine oxide completely eliminated the corrosion of the steel disposable scalpel blades caused by the sodium hypochlorite. 
     EXAMPLE II 
     Controlling Corrosion of Metals in NaOCl Solution 
     PURPOSE 
     The purpose is to establish the inhibiting effect of increasing concentrations of mono and dipotassium phosphate in sodium hypochlorite solutions, with and without a surface active agent. 
     MATERIALS 
     1. Sodium hypochlorite (Purex Bleach) 
     2. Monopotassium phosphate (KH 2  PO 4 ) 
     3. Dipotassium phosphate (K 2  HPO 4 ) 
     4. Dimethyl lauryl amine oxide (30% active) 
     5. Disposable scalpel blades, #21 (Fisher Scientific Co. Cat. #8-918-B) 
     TEST SOLUTIONS 
     A. 0.02% NaOCl (pH 10.05) 
     B. 0.02% NaOCl, 0.35% amine oxide (pH 9.8) 
     C. 0.02% NaOCl, 0.033% KH 2  PO 4  (pH 7.5) 
     D. 0.02% NaOCl, 0.033% KH 2  PO 4 , 0.35% amine oxide (pH 7.5) 
     E. 0.02% NaOCl, 0.25% K 2  HPO 4 , 0.058% KH 2  PO 4  (pH 7.5) 
     F. 0.02% NaOCl, 0.25% K 2  HPO 4 , 0.058% KH 2  PO 4 , 0.35% amine oxide (pH 7.5) 
     G. 0.02% NaOCl, 0.5% K 2  HPO 4 , 0.117% KH 2  PO 4  (pH 7.5) 
     H. 0.02% NaOCl, 0.5% K 2  HPO 4 , 0.117% KH 2  PO 4 , 0.35% amine oxide (pH 7.5) 
     I. 0.02% NaOCl, 1.0% K 2  HPO 4 , 0.192% KH 2  PO 4  (pH 7.5) 
     J. 0.02% NaOCl, 1.0% K 2  HPO 4 , 0.192% KH 2  PO 4 , 0.35% amine oxide (pH 7.5) 
     K. 0.02% NaOCl, 2.0% K 2  HPO 4 , 0.433% KH 2  PO 4  (pH 7.5) 
     L. 0.02% NaOCl, 2.0% K 2  HPO 4 , 0.433% KH 2  PO 4 , 0.35% amine oxide (pH 7.5) 
     PROCEDURE 
     Twelve pairs of #21 surgical disposable scalpel blades were accurately weighed on a micro balance. Each pair of blades was put into a separate test solution and the blades were allowed to soak for two hours. At the end of the soaking period, any corrosion products formed on the blades were washed off. The blades were then room air dried for at least one hour and each pair was reweighed on the micro balance. The weight loss for each pair of blades was computed. 
     
                       TABLE 5______________________________________RESULTSTestSolu- Initial   Final     Weight Visibletion  Weight    Weight    Loss   Observations______________________________________A&#39;    2061.79 mg           2059.29 mg                     2.50 mg                            general rustingB&#39;    2014.84 mg           2014.20 mg                     0.64 mg                            many small pits                            with rustC&#39;    2017.10 mg           2013.65 mg                     3.45 mg                            general rusting                            more than &#34;A&#34;D&#39;    2004.59 mg           2002.98 mg                     1.61 mg                            pitting and streak                            rustingE&#39;    1975.16 mg           1973.37 mg                     1.79 mg                            colorful streak-like                            corrosionF&#39;    1998.42 mg           1997.35 mg                     1.07 mg                            medium large pits,                            tarnishingG&#39;    1970.39 mg           1969.37 mg                     1.02 mg                            medium large pits,                            tarnishingH&#39;    1990.17 mg           1989.40 mg                     0.77 mg                            medium pits,                            tarnishedI&#39;    1998.35 mg           1997.64 mg                     0.71 mg                            small pits, some                            tarnishingJ&#39;    2018.52 mg           2018.02 mg                     0.05 mg                            few small pitsK&#39;    2006.45 mg           2006.31 mg                     0.14 mg                            two very small pitsL&#39;    1984.98 mg           1984.98 mg                       0 mg no corrosion______________________________________ 
    
     
                       TABLE 6______________________________________Test    % Corrosion Inhibition                    % Corrosion InhibitionSolution   Relative to &#34;A&#34;  Relative to &#34;C&#34;______________________________________A&#39;      --               --B&#39;      74.4%            --C&#39;      -38.0%           --D&#39;      35.6%            53.3%E&#39;      28.4%            48.1%F&#39;      57.2%            69.0%G&#39;      59.2%            70.4%H&#39;      69.2%            77.7%I&#39;      71.6%            79.4%J&#39;      80.0%            85.5%K&#39;      94.4%            95.9%L&#39;      100%             100%______________________________________ 
    
     
                       TABLE 3______________________________________                         ComparisonTest   % Corrosion Inhibition Compared                         SolutionSolution  To same solutions without Amine Oxide                         Code______________________________________B&#39;     74.4%                  A&#39;D&#39;     53.3%                  C&#39;F&#39;     40.2%                  E&#39;H&#39;     24.5%                  G&#39;J&#39;     29.6%                  I&#39;______________________________________ 
    
     CONCLUSION 
     A solution of 0.02% sodium hypochlorite in water was very corrosive to the steel scalpel blades. The addition of the amine oxide to the hypochlorite reduced the corrosion. Buffering the sodium hypochlorite solutions to pH 7.5 with monopotassium phosphate increased the corrosion by 38%. The addition of the amine oxide reduced corrosion of the buffered hypochlorite by 53.3%. The addition of increasing amounts of dipotassium phosphate to the sodium hypochlorite solution, and buffering the solution to a pH of 7.5 with monopotassium phosphate, provided increasing corrosion inhibition. In each solution studied, greater corrosion inhibition was obtained when the amine oxide was included. Solution &#34;L&#39;&#34; did not cause corrosion of the steel scalpel blades after a two hour exposure. 
     EXAMPLE III 
     Controlling Corrosion of Metals in NaOCl Solutions 
     PURPOSE 
     The purpose is to establish the minimum concentrations of phosphates needed to eliminate corrosion of surgical steel scalpel blades in a 0.02% sodium hypochlorite solution at pH 7.5. 
     MATERIALS 
     1. Sodium hypochlorite (from Purex Bleach) 
     2. Monopotassium phosphte (KH 2  PO 4 ) 
     3. Dipotassium phosphate (K 2  HPO 4 ) 
     4. Dimethyl lauryl amine oxide (30% active) 
     5. Disposable scalpel blades, #21 (Fisher Scientific Co. Cat. (#8-918-B) 
     TEST SOLUTIONS 
     R. 0.02% NaOCl, 0.025% KH 2  PO 4 , 0.35% amine oxide 
     S. 0.02% NaOCl, 1% K 2  HPO 4 , 0.167% KH 2  PO 4 , 0.35% amine oxide 
     T. 0.02% NaOCl, 1.2% K 2  HPO 4 , 0.233% KH 2  PO 4 , 0.35% amine oxide 
     U. 0.02% NaOCl, 1.4% K 2  HPO 4 , 0.267% KH 2  PO 4 , 0.35% amine oxide 
     V. 0.02% NaOCl, 1.6% K 2  HPO 4 , 0.33% KH 2  PO 4 , 0.35% amine oide 
     W. 0.02% NaOCl, 1.8% K 2  HPO 4 , 0.35% KH 2  PO 4 , 0.35% amine oxide 
     X. 0.02% NaOCl, 2.0% K 2  HPO 4 , 0.400% KH 2  PO 4 , 0.35% amine oxide 
     Z. 0.02% NaOCl, 2.2% K 2  HPO 4 , 0.433% KH 2  PO 4 , 0.35% amine oxide 
     PROCEDURE 
     Eight pairs of surgical disposable scalpel blades were accurately weighed on a Sartorius Microbalance. Each pair of blades was put into a separate test solution and the blades were allowed to soak for four hours. At the end of the soaking period, the blades were removed from the solutions and any corrosion products formed on the blades were washed off. The blades were then dried at room temperature for at least one hour and then reweighed. The weight loss for each pair of blades was computed. 
     
                       TABLE 3______________________________________RESULTS:TEST                              VISIBLESOLU- INITIAL   FINAL     WEIGHT  OBSER-TION  WEIGHT    WEIGHT    LOSS    VATIONS______________________________________R.    2018.69 mg           2016.20 mg                     2.49 mg much corrosionS     2002.82 mg           2002.65 mg                     0.17 mg few pitsT.    2009.89 mg           2009.82 mg                     0.07 mg two visible pitsU.    1984.31 mg           1984.29 mg                     0.02 mg no visible                             corrosionV.    1974.62 mg           1974.60 mg                     0.02 mg no visible                             corrosionW.    1982.88 mg           1982.87 mg                     0.01 mg no visible                             corrosionX.    2013.87 mg           2013.87 mg                       0 mg  no visible                             corrosionZ.    1967.18 mg           1967.17 mg                     0.01 mg no visible                             corrosion______________________________________ 
    
     
                       TABLE 4______________________________________    %TEST     INHIBITION OF CORROSION RELATIVESOLUTION TO TEST SOLUTION &#34;R&#34;______________________________________S.       93.2%T.       97.2%U.       99.2%V.       99.2%W.       99.6%X.        100%Z.       99.6%______________________________________ 
    
     CONCLUSION 
     No detectable corrosion was found in Solutions &#34;U&#34; through &#34;Y&#34;. A 93 to 97% reduction in corrosion occurred in solutions &#34;S&#34; and &#34;T&#34;. 
     EXAMPLE IV 
     Controlling Corrosion in NaOCl Solutions 
     PURPOSE 
     The purpose of this experiment is to establish the minimum concentration of phosphates needed to eliminate corrosion of surgical steel scalpel blades in a 0.5% sodium hypochlorite solution at pH 7.5. 
     MATERIALS 
     1. Sodium hypochlorite (from Purex Bleach) 
     2. Monopotassium phosphates (KH 2  PO 4 ) 
     3. Dipotassium phosphate (K 2  HPO 4 ) 
     4. Dimethyl lauryl amine oxide 
     5. Disposable scalpel blades, #21 (Fisher Scientific Co. Cat. #8-918-B) 
     TEST SOLUTIONS 
     M&#39; 0.5% NaOCl, 0.35% amine oxide, 0.500% KH 2  PO 4   
     N&#39; 0.5% NaOCl, 0.35% amine oxide, 1% K 2  HPO 4 , 0.633% KH 2  PO 4   
     O&#39; 0.5% NaOCl, 0.35% amine oxide, 2% K 2  HPO 4 , 0.967% KH 2  PO 4   
     P&#39; 0.5% NaOCl, 0.35% amine oxide, 4% K 2  HPO 4 , 1.40% KH 2  PO 4   
     R&#39; 0.5% NaOCl, 0.35% amine oxide, 6% K 2  HPO 4 , 2.00% KH 2  PO 4  * 
     S&#39; 0.5% NaOCl, 0.35% amine oxide, 8% K 2  HPO 4 , 2.53% KH 2  PO 4  ** 
     T&#39; 0.5% NaOCl, 0.35% amine oxide, 10% K 2  HPO 4 , 3.48% KH 2  PO 4  *** 
     U&#39; 0.5% NaOCl, 0.35% amine oxide, 12% K 2  HPO 4 , 3.77% KH 2  PO 4  **** 
     V&#39; 0.5% NaOCl, 0.35% amine oxide, 14% K 2  HPO 4 , 4.13% KH 2  PO 4  ***** 
     W&#39; 0.5% NaOCl, 0.35% amine oxide, 16% K 2  HPO 4 , 4.90% KH 2  PO 4  ****** 
    
    
    
    
    
    
    
     PROCEDURE 
     Ten pairs of surgical disposable scalpel blades were accurately weighed on a Sartorius Microbalance. Each pair of blades was immersed in separate test solutions and allowed to soak for two hours. At the end of the soaking period, the blades were removed from the test solutions and any corrosion products were washed off. The blades were then room air dried for at least one hour and then reweighed. The weight loss fo each pair of blades was computed. 
     
                                           TABLE 1__________________________________________________________________________RESULTS:TEST   INITIAL        FINAL WEIGHT                   VISIBLESOLUTION  WEIGHT        WEIGHT              LOSS OBSERVATIONS__________________________________________________________________________M&#39;     2043.15 mg        2033.52 mg               9.63 mg                   gross corrosionN&#39;     2033.28 mg        2022.29 mg              10.99 mg                   gross corrosion (worse                   than &#34;M&#34;)O&#39;     2024.60 mg        2018.04 mg              6.56 mg                   many large pitsP&#39;     2016.91 mg        2016.81 mg              0.10 mg                   few very small pitsR&#39;     2022.33 mg        2022.25 mg              0.08 mg                   very few very small pitsS&#39;     2034.16 mg        2034.10 mg              0.06 mg                   3 or 4 tiny pitsT&#39;     2016.97 mg        2016.97 mg                0 mg                   no visible corrosionU&#39;     1993.69 mg        1993.69 mg                0 mg                   no visible corrosionV&#39;     1985.00 mg        1985.00 mg                0 mg                   no visible corrosionW&#39;     2000.89 mg        2000.89 mg                0 mg                   no visible corrosion__________________________________________________________________________ 
    
     
                       TABLE 9______________________________________                     % CORROSION                     INHIBITION                     COMPARED TOTEST    TOTAL % PHOSPHATES                     TEST SOLUTIONSOLUTION   AS H2PO4 AND HPO4 &#34;M&#34;______________________________________M&#39;      0.390%            --N&#39;      1.04%             -14.1%O&#39;      1.86%             31.9%P&#39;      3.29%             99.0%R&#39;      4.86%             99.2%S&#39;      6.38%             99.4%T&#39;      8.22%              100%U&#39;      9.55%              100%V&#39;      10.93%             100%W&#39;      12.63%             100%______________________________________ 
    
     CONCLUSION 
     Complete corrosion inhibition of surgical steel scalpel blades in 0.5% sodium hypochlorite was obtained by the addition of 6.38% (and greater) total phosphates as H 2  PO 4  and HPO 4 . 
     EXAMPLE V 
     Sterilization Testing of Test Solution Q 
     PURPOSE 
     To demonstrate that a one day old test solution can sterilize one million spores of Bacillus subtilis var. globigii inoculated from a hard water suspension onto aluminum foil carriers. 
     MATERIALS 
     1. Test Solution Q--activated with hard water 
     0.2% sodium hypochlorite 
     0.35% dimethyl lauryl amine oxide (30% active) 
     9% dipotassium phosphate 
     2% monopotassium phosphate 
     2. Bacillus subtilis var. globigii spores in ethanol 
     3. Aluminum foil strips 
     4. Culture broth--formula in grams per liter 
     5 gm peptone 
     3 gm beef extract 
     3 gm yeast extract 
     2 gm dextrose 
     3 gm sodium sulfite 
     3 gm ammonium sulfate 
     10 ml Tween 80 
     0.7 gm lecithin 
     5. Hard water--0.304 gm CaCl 2+0 .14 gm Mgcl 2  6 H 2  O/Liter 
     METHOD 
     1. A quantity of spores in ethanol was centrifuged at 3000 rpm for 20 minutes and the ethanol was decanted. The spores were resuspended in sufficient sterile hard water so that the spore count was approximately 2,000,000 spores per 0.05 ml. of inoculum. 
     2. Each of 25 aluminum foil carriers was inoculated with 0.05 ml of the spore/hard water suspension. The inoculum was allowed to dry onto the carriers at room temperature for five days. 
     3. Twenty inoculated and dried carriers were immersed in a one day old Test Solution Q. The carriers were removed after 15 minutes of exposure and aseptically transferred to individual tubes of culture broth. All cultures were incubated for seven days at 37° C. 
     4. 0.2 ml of Test Solution Q was added to a tube of culture broth. One inoculated and dried carrier was added to the culture tube and incubated at 37° C. The purpose of this test was to determine if there was any growth inhibitory effect of the solution on the germinating spores. 
     5. One tube of culture broth was incubated with an inoculated carrier to serve as a positive control. 
     6. The number of viable spores on a carrier was determined by washing the spores off of the carrier with an ultrasonic cleaner into a 0.5% Tween 80 solution, and counting the spores in solution by the dilution/agar pour plate method. 
     RESULTS 
     Viable spore count--1,500,000 spores per carrier 
     Positive control--growth of Bacillus globigii 
     Inhibition control--growth of Bacillus globigii 
     Carriers exposed to Test Solution Q--no growth of Bacillus globiggi in any of the 20 cultures. 
     CONCLUSION 
     No growth of Bacillus subtilis var. globigii occurred in any of the twenty tubes of culture broth incubated with aluminum foil carriers exposed to a one day old Test Solution Q for 15 minutes. The growth of Bacillus globigii which occurred in the culture tube inoculated with 0.2 ml of Test Solution Q and incubated with an inoculated carrier, demonstrated that all of the solution capable of exerting a growth inhibiting effect on the germinating spores had been effectively neutralized by the culture broth. 
     It is therefore concluded that all twenty aluminum foil carriers inoculated with a hard water suspension of Bacillus subtilis var. globigii were successfully sterilized by Test Solution Q using a 15 minute exposure time. 
     EXAMPLE VI 
     Disinfection of Salmonella with Test Solution Q 
     PURPOSE 
     To demonstrate that a two day old Test Solution Q can disinfect Salmonella choleraesuis, inoculated from a hard water suspension onto stainless steel penicylinders, using a one minute exposure time. 
     MATERIALS 
     1. Test Solution Q--activated using hard water 
     2. Salmonella choleraesuis A.T.C.C. #10708 
     3. Stainless steel penicylinders (Fisher Cat. #7-907-5) 
     4. 0.1% Asparagine (Bacto) 
     5. Nutrient broth--formula in grams per liter 
     10 gm peptic digest of animal tissue 
     5 gm beef extract 
     5 gm sodium chloride 
     6. Culture broth--formula in grams per liter 
     5 gm peptone 
     3 gm beef extract 
     3 gm yeast extract 
     2 gm dextrose 
     3 gm sodium sulfite 
     3 gm ammonium sulfate 
     10 ml Tween 80 
     0.7 gm Lecithin 
     7. Hard water--formula in grams per liter 
     0.304 gm Calcium chloride 
     0.139 gm Magnesium chloride hexahydrate 
     METHOD 
     1. 65 stainless steel penicylinders were washed in 1 M NaOH and rinsed thoroughly. The washed penicylinders were placed in a flask, covered with 0.1% Asparagine, and autoclaved for 15 minutes at 121 degrees C. 
     2. The contents of six tubes, each containing 10 ml of a 48 hour nutrient broth culture of Salmonella choleraesuis, were aseptically poured into sterile centrifuge tubes. The culture was centrifuged at 3000 rpm for 15 minutes and the supernatant liquid was decanted. The microorganisms were suspended in 60 ml of sterile hard water. 
     3. The penicylinders were aseptically transferred to the hard water bacterial suspension and were allowed to soak in the suspension for 15 minutes. The penicylinders were then removed and placed vertically in a sterile petri dish matted with two sterile circles of filter paper. The inoculated penicylinder were dried in an incubator at 37 degrees C. for 25-35 minutes. 
     4. Sixty inoculated and dried penicylinders were soaked in a two day old Test Solution Q for 1 minute and transferred to individual tubes of culture broth. All cultures were incubated at 37 degrees C. for 3 days. 
     5. One penicylinder, unexposed to Test Solution Q, was placed in a culture broth tube and incubated to serve as a positive control. 
     6. 0.2 ml of Test Solution Q was added to a tube of culture broth. One penicylinder was then placed in the broth and incubated. This test served to detect unneutralized chlorine the might inhibit growth of microorganisms. 
     7. One inoculated and dried penicylinder was shaken in 9.0 ml of sterile saline for three minutes. The number of viable microorganisms in the resulting solution was determined by the dilution/agar pour plate method. 
     RESULTS 
     Viable microorganisms on a penicylinder=300,000 
     Positive control--positive growth of Salmonella 
     Inhibition control--positive growth of Salmonella 
     60 penicylinders exposed Test Solution Q for one minute-- 
     No growth of Salmonella in 59 cultures 
     Growth of Salmonella occured in 1 culture 
     CONCLUSION 
     Growth of Salmonella choleraesuis occurred in only one of the sixty tubes containing penicylinders exposed to Test Solution Q. No inhibition was noted due to unneutralized solution. It is therefore concluded that 59 of the 60 stainless steel penicylinders, inoculated with 300,000 organisms of Salmonella choleraesuis in a hard water suspension, were successfully sterilized by Test Solution Q using a one minute exposure. 
     EXAMPLE VII 
     Kelsey-Sykes &#34;Clean&#34; Disinfectant Test using Test Solution Q 
     MATERIALS 
     1. Test Solution Q 
     2. Test Organisms 
     a. Staphylococcus aureus A.T.C.C. #6538 
     b. Pseudomonas aeruginosa A.T.C.C. #15542 
     c. Pseudomonas aeruginosa A.T.C.C. #27835 
     d. Escherichia coli A.T.C.C. #25922 
     e. Salmonella choleraesius A.T.C.C. #10708 
     f. Klebsiella pneumoniae A.T.C.C. #13883 
     3. Medium for growth of test organisms--Bacto Synthetic Broth AOAC Code No. 0352 to which 0.1 ml of 10% dextrose has been added per 10 ml tube of broth. 
     4. Recovery Broth--Nutrient broth (BBL) containing 3% Tween 80 (10 ml per tube) 
     5. Standard Hard Water--0.304 gm CaCl 2 , 0.139 gm MgCl 2  6H 2  O/liter 
     METHOD 
     1. The Test Solution Q was diluted and activated by adding 3 parts of standard hard water to one part of Test Solution Q concentrate. The following test was performed using each of the microorganisms cited in &#34;Materials&#34;, on a one day and a two day old Activated Test Solution Q. 
     2. Daily subcultures of each test organism were grown in 10 ml quantities of Synthetic broth. The subcultures were incubated for 24 hours at 37 degrees C. Daily subcultures were made for at least five days but less than fourteen days. 
     3. A 24 hour subculture of each test organism was centrifuged at 3,000 rpm for 15 minutes and the supernatant liquid was removed. Each test organism was resuspended in 10 ml of sterile standard hard water and shaken for one minute with a few glass beads. The 10 ml water/organism suspension of each test organism was used as the inoculum for the test. 
     4. The following procedure was used for each test organism. 
     5. Three ml of Activated Test Solution Q were pipetted into a 50 ml uncovered glass beaker. One ml of test inoculum was added to the Test Solution Q and the contents were mixed. Thirty seconds after the addition, 0.02 ml of the Test Solution Q/inoculum mixture was added to each of five recovery broths. 
     6. Ten minutes after the first addition, a second one ml inoculum was added to the Test Solution Q mixture and the contents were shaken. Thirty seconds after the second addition, 0.02 ml of the Test Solution Q mixture was added to each of five recovery broths. 
     7. Twenty minutes after the first addition, a third one ml inoculum was added to the Test Solution Q mixture and the contents were shaken. Thirty seconds after the third addition, 0.02 ml of the Test Solution Q mixture was added to each of five recovery broths. 
     8. All inoculated recovery broths were incubated at 37 degrees C. for 48 hours. 
     9. A microorganism count was performed on each test organism inoculum by dilution in sterile water and preparation of agar pour plates of each dilution. Colonies were counted after 2 days incubating at 37 degrees C. 
     10. All recovery broths showing no growth after 2 days of incubation were inoculated with a 1:1000 dilution of test organism culture and reincubated for an additional 1 day. This test served to show the presence of any inhibitory substance transferred to the recovery broth tubes. Positive growth indicates no inhibition. 
     
         ______________________________________RESULTS:Culture Codes:       + = positive growth       0 = no growthTest                         Recovery BrothsSolution O   Test Organism              Count/ml  (1)   (2)   (3)______________________________________1 day   Staph      5.6 × 10.sup.8                        00000 00000 00000   Pseudomonas              3.5 × 10.sup.9                        00000 00000 00000   15542   Pseudomonas              9.8 × 10.sup.8                        00000 00000 00000   27835   E. coli    5.5 × 10.sup.8                        00000 00000 00000   Salmonella 1.0 × 10.sup.9                        00000 00000 00000   Klebsiella 3.0 × 10.sup.9                        00000 00000 00000Inhibitory Test - all recovery broth tubes positive______________________________________2 days  Staph      3.5 × 10.sup.8                        00000 00000 00000   Pseudomonas              5.6 × 10.sup.8                        00000 00000 00000   15542   Pseudomonas              8.7 × 10.sup.8                        00000 00000 00000   27835   E. coli    4.3 × 10.sup.8                        00000 00000 00000   Salmonella 7.4 × 10.sup.8                        00000 00000 00000   Klebsiella 1.2 × 10.sup.9                        00000 00000 00000Inhibitory Test - all recovery broth tubes positive______________________________________ 
    
     CONCLUSION 
     Both a one day and a two day old Test Solution Q passed the Kelsey-Sykes Disinfectant test under &#34;clean&#34; conditions using a 30 second exposure time. 
     EXAMPLE VIII 
     The effect of the concentration of dimethyl lauryl amine oxide on silk loop sinking time 
     PURPOSE 
     To determine the optimal concentration of dimethyl lauryl amine oxide necessary to rapidly eliminate air bubbles from standard A.O.A.C. style silk suture loops and cause them to sink in an aqueous solution. 
     MATERIALS 
     1. Dimethyl lauryl amine oxide (30% active) 
     2. Tap water 
     3. Silk suture loops (prepared according to the A.O.A.C. specifications) 
     4. Stop watch 
     5. Beakers 
     PROCEDURE 
     1. Concentrations of 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4 and 0.5% of amine oxide in tap water were prepared. 
     2. A suture loop sinking time test was performed on each solution. A stop watch was used to determine the interval between the time a suture loop was submerged in a test solution and the time at which the loop reached the bottom of the beaker. 
     3. At least four suture loops were tested in each solution. The average of all of the test times for each solution was determined. 
     
         ______________________________________RESULTS:(Average of 4 or more tests per solution)Concentration of Amine Oxide            Average Sinking Time(seconds)______________________________________ 0.10%           620.15             230.20             170.25             190.30             130.35             110.40             100.50             7______________________________________ 
    
     CONCLUSION 
     A rapid decrease in sinking time occurred when the concentration of amine oxide was increased from 0.1 to 0.2%. above 0.3%, smaller additional decreases in sinking times were noted. 
     EXAMPLE IX 
     Eye Irritation Testing of Test Solution Q 
     PURPOSE 
     The purpose of this test was to determine if Test Solution Q is irritating to the eye when tested on rabbits using the method described by Dr. J. H. Draize. 
     MATERIALS 
     1. Three healthy New Zealand white rabbits 
     2. Test Solution Q 
     PROCEDURE 
     1. One tenth milliliter of freshly mixed Test Solution Q was instilled in the conjunctival sac of one eye in each of the three rabbits, with the other eye of each rabbit serving as the control. The treated eyes were not washed. Ocular reactions read at 24 and 48 hours. 
     2. Six days after the first treatment, two separate hourly conjunctival instillations of one tenth milliliter of freshly mixed Test Solution Q were performed using the same test eyes as in the first treatment. Again, the treated eyes were not washed. Ocular reactions were read at 24 and 48 hours. 
     3. Two days after the second treatment, three separate hourly conjunctival instillations of one tenth milliliter of freshly mixed Test Solution Q were performed using the same test eyes as in the first treatment. Again, the treated eyes were not washed. Ocular reactions were read at 24 and 48 hours. 
     4. Reactions were scored using the method suggested by Dr. Draize. 
     CONCLUSION 
     On the basis of the data presented herein, Test Solution Q is not an irritant to the rabbit eye when tested according to the method suggested by Dr. J. H. Draize and described in &#34;Appraisal of the safety of Chemicals in Food, Drugs and Cosmetics&#34;, published by the Association of Food and Drug Officials of the United States. 
     
         __________________________________________________________________________Rabbits #1 and #3  First, Second and Third Treatment Results__________________________________________________________________________Days After Instillation:            1 2I. CorneaA.   Opacity--Degree of Density (area which is most dense istaken for reading)Scattered or diffuse area--details of iris clearly                             (1)ible                               0 0B.   Area of Cornea InvolvedOne quarter (or less) but not zero                             (1)                               0 0Score equals A × B × 5  Total maximum = 80                          0 × 0 × 5 = 0II.   IrisA.   ValuesFolds above normal, congestion, swelling, circumcornealinjection (any one or all of these or combination of anythereof), iris still reacting to light (sluggish reactionif positive)                 (1)                               0 0Score equals A × 5  Total possible maximum = 10                          0 × 5 = 0III.   ConjunctivaeA.   Redness (refers to palpebral conjunctivae only)Vessels definitely injected above normal                             (1)                               0 0B.   ChemosisAny swelling above normal (includes nictitating membrane)                             (1)                               0 0C.   DischargeAny amount different from normal (does not include smallamount observed in inner canthus of normal animals)                             (1)                               0 0Score (A + B + C) × 2  Total maximum + 20                        (0 + 0 + 0) × 2 = 0__________________________________________________________________________ 
    
     
         __________________________________________________________________________Rabbit #2  First and Second Treatment Results__________________________________________________________________________Days After Instillation:               1 2I. CorneaA.   Opacity--Degree of Density (area which is most dense istaken for reading)Scattered or diffuse area-details of iris clearly visible.                                (1)                                  0 0B.   Area of Cornea InvolvedOne quarter (or less) but not zero                                (1)                                  0 0Score equals A × P × 5   Total maximum = 80                          0 × 0 × 5 = 0II.   IrisA.   ValuesFolds above normal, congestion, swelling, circumcorneal injection(any one or all of these or combination of any thereof), irisstill reacting to light (sluggish reaction if positive)                                (1)                                  0 0Score equals A × 5  Total possible maximum = 10                          0 ×0 5 = 0III.   ConjunctivaeA.   Redness (refers to palpebral conjunctivae only)Vessels definitely injected above normal                                (1)                                  0 0B.   ChemosisAny swelling above normal (includes nictitating membrane).                                (1)                                  0 0C.   DischargeAny amount different from normal (does not include smallamount observed in inner canthus of normal animals)                                (1)                                  0 0Score (A + B + C) × 2  Total maximum = 20                          (0 + 0 + 0) × 2 = 0__________________________________________________________________________ 
    
     
         __________________________________________________________________________Rabbit #2  Third Treatment ResultsDays After Instillation:             1  2__________________________________________________________________________I. CorneaA.   Opacity--Degree of Density (area which is most denseis taken for readingScattered or diffuse are--details of iris clearlyvisible                     (1) 0  0B.   Area of Cornea InvolvedOne quarter (or less) but not zero                            (1) 0  0Score equals A × P × 5 Total maximum = 80                            0 × 0 × 5 = 0II.   IrisA.   ValuesFolds above normal, congestion, swelling, circumcornealinjection (any one or all of these or combination of anythereof), iris still reacting to light (sluggish reactionif positive)                (1) 0  0Score equals A × 5 Total possible maximum = 10                            0 × 5 = 0III.   ConjunctivaeA.   Redness (refers to palpebral conjunctivae only)Vessels definitely injected above normal                            (1) 0  0B.   ChemosisAny swelling above normal (includes nictitatingmembrane)                   (1) 0  0C.   DischargeAny amount different from normal (does not include smallamount observed in inner canthus of normal animals).                            (1) 1  0Score (A + B + C) = 2 Total maximum = 20                            (1 + 0 + 0) × 2 = 2__________________________________________________________________________ 
    
     The multi-compartment pouch of this invention can be used for purposes other than an instrument sterilizing solution. For example, it may be used in conjunction with an antimicrobial agent. Thus, it is effective for storing other germicidal solutions, such as disinfectants for bodily tissues, the ingredients of which must be kept separated until mixed for use. Further, it is to be understood that the location of the solutions in the compartments can be varied and be within the scope of this invention. For example, the phosphate solution could be put in the middle compartment with a color indicator which disappears when contact with the hypochlorite occurs. As desired, the coloring agents may be omitted or substituted by other agents which are nonreactive to the other ingredients, in as much as the coloring agent is merely a tell-tale device. Also, any number of plurality of compartments may be utilized. 
     It will be understood that utilizing the first bath with the conditioning agents is not always essential, because the second chlorine-based sterilizing liquid bath, used alone, can still perform its function with its effectiveness being a matter of degree depending on the condition of the items being sterilized and their need for pre-conditioning treatment. 
     Although dimethyl lauryl amine oxide has been indicated as 30% active because of its commercial availability, other concentrations may be utilized and the amounts proportioned accordingly. 
     It is to be understood that the multi-compartment pouch of this invention, prior to filling with liquid components and complete sealing, is in and of itself an article of commerce. Thus the multi-compartment pouch may be initially made with filling openings for each compartment and subsequently sealed after being filled with the desired contents. For example, three of the side seams may be initially sealed before filling, and the final and fourth side seams sealed after filling. Pouches with other than four side seams may also be used.