Abstract:
The current invention relates to a diagnostic kit for a yeast or fungal species comprising at least one oligonucleotide probe capable of binding to at least a portion of the eIF2y gene or its corresponding mRNA.

Description:
FIELD OF THE INVENTION  
       [0001]    The present invention relates to nucleic acid primers and probes to detect one or more fungal and yeast species. More specifically the invention relates to the eIF2γ gene (also known as the EF-2 gene), the corresponding RNA, specific probes, primers and oligonucleotides related thereto and their use in diagnostic assays to detect and/ or discriminate between fungal and yeast species. 
       BACKGROUND OF THE INVENTION  
       [0002]    Yeast and fungal infections represent a major cause of morbidity and mortality among immunocompromised patients. The number of immunocompromised patients at risk of yeast and fungal infection continues to increase each year, as does the spectrum of fungal and yeast agents causing disease. Mortality from fungal infections, particularly invasive fungal infections, is 30% or greater, in certain risk groups. The array of available antifungal agents is growing; however, so too is the recognition of both intrinsic and emerging resistance to antifungal drugs. These factors are contributing to the increased need for cost containment in laboratory testing and have led to laboratory consolidation in testing procedures. 
         [0003]    Invasive fungal infections are on the increase. In 2003, it was estimated that there were 9 million at risk patients, of which 1.2 million developed infection.  Candida  spp. and  Aspergillus  spp. now rank as the most prominent pathogens infecting immunosupressed patients. In particular, infections are common in the urinary tract, the respiratory system and the bloodstream, at the site of insertion of stents, catheters and orthopaedic joints. Approximately, 10% of the known  Candida  spp. have been implicated in human infection. Invasive candidiasis occurs when candida enters the bloodstream and it is estimated to occur at a frequency of 8/100,000 population in the US with a mortality rate of 40%.  Candida albicans  is the 4 th  most common cause of bloodstream infection. Aspergillosis usually begins as a pulmonary infection that can progress to a life-threatening invasive infection in some patients and has a mortality rate of greater than 90%. Emerging mycoses agents include  Fusarium, Scedosporium, Zygomycetes  and  Trichosporon  spp. (“ Stakeholder Insight: Invasive fungal infections” , Datamonitor, January 2004). 
         [0004]    Immunocompromised patients, including transplant and surgical patients, neonates, cancer patients, diabetics and those with HIV/AIDs are at high risk of developing invasive fungal infections (Datamonitor report: Stakeholder opinion—Invasive fungal infections, options outweigh replacements 2004). A large number of severe cases of sepsis are reported each year. Despite improvements in its medical management, sepsis still constitutes one of the greatest challenges in intensive care medicine. Microorganisms (bacteria, fungi and yeast) responsible for causing sepsis are traditionally detected in hospital laboratories with the aid of microbiological culture methods with poor sensitivity (25-82%), which are very time-consuming, generally taking from two to five days to complete, and up to eight days for the diagnosis of fungal infections. Definitive diagnosis of infections caused by yeast or fungi is usually based on either, the recovery and identification of a specific agent from clinical specimens or microscopic demonstration of fungi with distinct morphological features. However, there are numerous cases where these methods fail to provide conclusive proof as to the infecting agent. In these instances, the detection of specific host antibody responses can be used, although again this can be affected by the immune status of the patient. Time is critical in the detection and identification of bloodstream infections typically caused by bacteria, yeasts or fungi. Effective treatment depends on finding the source of infection and making appropriate decisions about antibiotics or antifungals quickly and efficiently. Only after pathogens are correctly identified can targeted therapy using a specific antibiotic or antifungal begin. Many physicians would like to see the development of better in vitro amplification and direct detection diagnostic techniques for the early diagnosis of yeast and fungi (“ Stakeholder Insight: Invasive fungal infections” , Datamonitor, January 2004). Recently Roche™ launched a real time PCR based assay (Septifast™), for the detection of bacterial, fungal and yeast DNA in clinical samples. Therefore, there is a clear need for the development of novel rapid diagnostic tests for clinically significant bacterial and fungal pathogens for bioanalysis applications in the clinical sector. This has led the current inventors to identify novel fungal and yeast nucleic acid targets for application in Nucleic Acid Diagnostics (NAD) tests. 
         [0005]    Fungal and yeast nucleic acid based diagnostics have focused heavily on the ribosomal RNA (rRNA) genes, RNA transcripts, and their associated DNA/RNA regions. The rRNA genes are highly conserved in all fungal species and they also contain divergent and distinctive intergenic transcribed spacer regions. Ribosomal rRNA comprises three genes: the large sub-unit gene (28S), the small sub-unit gene (18S) and the 5.8S gene. The 28S and 18S rRNA genes are separated by the 5.8S rRNA and two internal transcribed spacers (ITS1 and ITS2). Because the ITS region contains a high number of sequence polymorphisms, numerous researchers have concentrated their efforts on these as targets (Atkins and Clark, 2004). rRNA genes are also multicopy genes with &gt;10 copies within the fungal genome. 
         [0006]    A number of groups are working on developing new assays for fungal and yeast infections. US2004044193 relates to, amongst a number of other aspects, the transcription factor CaTEC1 of  Candida albicans;  inhibitors thereof, and methods for the diagnosis and therapy of diseases which are connected with a  Candida  infection; and also diagnostic and pharmaceutical compositions which contain the nucleotide sequences, proteins, host cells and/or antibodies. WO0183824 relates to hybridization assay probes and accessory oligonucleotides for detecting ribosomal nucleic acids from  Candida albicans  and/or  Candida dubliniensis.  U.S. Pat. No. 6,017,699 and U.S. Pat. No. 5,426,026 relate to a set of DNA primers, which can be used to amplify and speciate DNA from five medically important  Candida  species. U.S. Pat. No. 6,747,137 discloses sequences useful for diagnosis of  Candida  infections. EP 0422872 and U.S. Pat. No. 5,658,726 disclose probes based on 18S rRNA genes, and U.S. Pat. No. 5,958,693 discloses probes based on 28S rRNA, for diagnosis of a range of yeast and fungal species. U.S. Pat. No. 6,017,366 describes sequences based on chitin synthase gene for use in nucleic acid based diagnostics for a range of  Candida  species. 
         [0007]    It is clear though, that development of faster, more accurate diagnostic methods are required, particularly in light of the selection pressure caused by modern antimicrobial treatments which give rise to increased populations of resistant virulent strains with mutated genome sequences. Methods that enable early diagnosis of microbial causes of infection enable the selection of a specific narrow spectrum antibiotic or antifungal to treat the infection (Datamonitor report: Stakeholder opinion—Invasive fungal infections, options outweigh replacements 2004; Datamonitor report: Stakeholder Opinion-Sepsis, under reaction to an overreaction, 2006). Eukaryotic initiation factor 2 (eIF2) is a heterotrimer composed of three subunits eIF2alpha (eIF2α), eIF2beta (eIF2β) and eIF2gamma (eIF2γ). eIF2 is the eukaryotic translation initiation factor 2, which is a heterotrimeric G-protein required for GTP-dependent delivery of initiator tRNA to the ribosome. The eIF2 gamma subunit (eif2γ) has a similar amino acid sequence to prokaryotic translation elongation factor EF-Tu which was patented as molecular diagnostics targets for micro-organisms (Alone and Dever, 2006; Dorris et al., 1995; Erickson et al., 1996 and 1997). 
         [0008]    There are currently 171 sequences of eIF2γ available in NCBI GenBank database including 3  Candida  spp. including 2 annotated eIF2γ sequences for  C. albicans  and one hypothetical protein for  C. glabrata  with 78% homology to  C. albicans  eIF2γ and 6  Aspergillus  spp. sequences, 3 annotated as eIF2γ and 3 hypothetical sequences. The published sequences are approximately 1600 base pairs in length providing a number of sequence regions that are suitable for PCR primer and probe design for species identification of  Candida  and  Aspergillus  spp. For  Candida,  the inventors focussed on the region of the eIF2 gamma gene equivalent to base pair (bp) position 718 to by position 1040 in  C. albicans.  For  Aspergillus,  the inventors designed primers to amplify the region of the eIF2γ gene equivalent to by position 121 to by position 374 in  Aspergillus fumigatus.    
         [0009]    Definitions 
         [0010]    “Synthetic oligonucleotide” refers to molecules of nucleic acid polymers of 2 or more nucleotide bases that are not derived directly from genomic DNA or live organisms. The term synthetic oligonucleotide is intended to encompass DNA, RNA, and DNA/RNA hybrids that have been manufactured chemically, or synthesized enzymatically in vitro. 
         [0011]    An “oligonucleotide” is a nucleotide polymer having two or more nucleotide subunits covalently joined together. Oligonucleotides are generally about 10 to about 100 nucleotides. The sugar groups of the nucleotide subunits may be ribose, deoxyribose, or modified derivatives thereof such as OMe. The nucleotide subunits may be joined by linkages such as phosphodiester linkages, modified linkages or by non-nucleotide moieties that do not prevent hybridization of the oligonucleotide to its complementary target nucleotide sequence. Modified linkages include those in which a standard phosphodiester linkage is replaced with a different linkage, such as a phosphorothioate linkage, a methylphosphonate linkage, or a neutral peptide linkage. Nitrogenous base analogs also may be components of oligonucleotides in accordance with the invention. 
         [0012]    A “target nucleic acid” is a nucleic acid comprising a target nucleic acid sequence. A “target nucleic acid sequence,” “target nucleotide sequence” or “target sequence” is a specific deoxyribonucleotide or ribonucleotide sequence that can be hybridized to a complementary oligonucleotide. 
         [0013]    An “oligonucleotide probe” is an oligonucleotide having a nucleotide sequence sufficiently complementary to its target nucleic acid sequence to be able to form a detectable hybrid probe:target duplex under high stringency hybridization conditions. An oligonucleotide probe is an isolated chemical species and may include additional nucleotides outside of the targeted region as long as such nucleotides do not prevent hybridization under high stringency hybridization conditions. Non-complementary sequences, such as promoter sequences, restriction endonuclease recognition sites, or sequences that confer a desired secondary or tertiary structure such as a catalytic active site can be used to facilitate detection using the invented probes. An oligonucleotide probe optionally may be labelled with a detectable moiety such as a radioisotope, a fluorescent moiety, a chemiluminescent, a nanoparticle moiety, an enzyme or a ligand, which can be used to detect or confirm probe hybridization to its target sequence. Oligonucleotide probes are preferred to be in the size range of from about 10 to about 100 nucleotides in length, although it is possible for probes to be as much as and above about 500 nucleotides in length, or below 10 nucleotides in length. 
         [0014]    A “hybrid” or a “duplex” is a complex formed between two single-stranded nucleic acid sequences by Watson-Crick base pairings or non-canonical base pairings between the complementary bases. “Hybridization” is the process by which two complementary strands of nucleic acid combine to form a double-stranded structure (“hybrid” or “duplex”). A “fungus” or “yeast” is meant any organism of the kingdom Fungi, and preferably, is directed towards any organism of the phylum Ascomycota. 
         [0015]    “Complementarity” is a property conferred by the base sequence of a single strand of DNA or RNA which may form a hybrid or double-stranded DNA:DNA, RNA:RNA or DNA:RNA through hydrogen bonding between Watson-Crick base pairs on the respective strands. Adenine (A) ordinarily complements thymine (T) or uracil (U), while guanine (G) ordinarily complements cytosine (C). 
         [0016]    The term “stringency” is used to describe the temperature, ionic strength and solvent composition existing during hybridization and the subsequent processing steps. Those skilled in the art will recognize that “stringency” conditions may be altered by varying those parameters either individually or together. Under high stringency conditions only highly complementary nucleic acid hybrids will form; hybrids without a sufficient degree of complementarity will not form. Accordingly, the stringency of the assay conditions determines the amount of complementarity needed between two nucleic acid strands forming a hybrid. Stringency conditions are chosen to maximize the difference in stability between the hybrid formed with the target and the non-target nucleic acid. 
         [0017]    With “high stringency” conditions, nucleic acid base pairing will occur only between nucleic acid fragments that have a high frequency of complementary base sequences (for example, hybridization under “high stringency” conditions, may occur between homologs with about 85-100% identity, preferably about 70-100% identity). With medium stringency conditions, nucleic acid base pairing will occur between nucleic acids with an intermediate frequency of complementary base sequences (for example, hybridization under “medium stringency” conditions may occur between homologs with about 50-70% identity). Thus, conditions of “weak” or “low” stringency are often required with nucleic acids that are derived from organisms that are genetically diverse, as the frequency of complementary sequences is usually less. 
         [0018]    ‘High stringency’ conditions are those equivalent to binding or hybridization at 42° C. in a solution consisting of 5× SSPE (43.8 g/l NaCl, 6.9 g/l NaH 2 PO 4 H 2 O and 1.85 g/l EDTA, ph adjusted to 7.4 with NaOH), 0.5% SDS, 5× Denhardt&#39;s reagent and 100 μg/ml denatured salmon sperm DNA followed by washing in a solution comprising 0.1× SSPE, 1.0% SDS at 42° C. when a probe of about 500 nucleotides in length is used. 
         [0019]    “Medium stringency’ conditions are those equivalent to binding or hybridization at 42° C. in a solution consisting of 5× SSPE (43.8 g/l NaCl, 6.9 g/l NaH 2 PO 4 H 2 O and 1.85 g/l EDTA, pH adjusted to 7.4 with NaOH), 0.5% SDS, 5× Denhardt&#39;s reagent and 100 μg/ml denatured salmon sperm DNA followed by washing in a solution comprising 1.0× SSPE, 1.0% SDS at 42° C., when a probe of about 500 nucleotides in length is used. 
         [0020]    ‘Low stringency’ conditions are those equivalent to binding or hybridization at 42° C. in a solution consisting of 5× SSPE (43.8 g/l NaCl, 6.9 g/l NaH 2 PO 4 H 2 O and 1.85 g/l EDTA, pH adjusted to 7.4 with NaOH), 0.1% SDS, 5× Denhardt&#39;s reagent [50× Denhardt&#39;s contains per 500 ml: 5 g Ficoll (Type 400, Pharamcia), 5 g BSA (Fraction V; Sigma)] and 100 μg/ml denatured salmon sperm DNA followed by washing in a solution comprising 5× SSPE, 0.1% SDS at 42° C., when a probe of about 500 nucleotides in length is used. 
         [0021]    In the context of nucleic acid in-vitro amplification based technologies, “stringency” is achieved by applying temperature conditions and ionic buffer conditions that are particular to that in-vitro amplification technology. For example, in the context of PCR and real-time PCR, “stringency” is achieved by applying specific temperatures and ionic buffer strength for hybridisation of the oligonucleotide primers and, with regards to real-time PCR hybridisation of the probe/s, to the target nucleic acid for in-vitro amplification of the target nucleic acid. 
         [0022]    One skilled in the art will understand that substantially corresponding probes of the invention can vary from the referred-to sequence and still hybridize to the same target nucleic acid sequence. This variation from the nucleic acid may be stated in terms of a percentage of identical bases within the sequence or the percentage of perfectly complementary bases between the probe and its target sequence. Probes of the present invention substantially correspond to a nucleic acid sequence if these percentages are from about 100% to about 80% or from 0 base mismatches in about 10 nucleotide target sequence to about 2 bases mismatched in an about 10 nucleotide target sequence. In preferred embodiments, the percentage is from about 100% to about 85%. In more preferred embodiments, this percentage is from about 90% to about 100%; in other preferred embodiments, this percentage is from about 95% to about 100% 
         [0023]    By “sufficiently complementary” or “substantially complementary” is meant nucleic acids having a sufficient amount of contiguous complementary nucleotides to form, under high stringency hybridization conditions, a hybrid that is stable for detection. Substantially complementary to can also refer to sequences with at least 90% identity to, e.g., 95, 96, 97, 98, 99, or 100% identity to, a given reference sequence. 
         [0024]    The terms “identical” or percent “identity,” in the context of two or more nucleic acids or polypeptide sequences, refer to two or more sequences or subsequences that are the same or have a specified percentage of amino acid residues or nucleotides that are the same (i.e., 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or higher identity over a specified region, when compared and aligned for maximum correspondence over a comparison window or designated region) as measured using a BLAST or BLAST 2.0 sequence comparison algorithms with default parameters described below, or by manual alignment and visual inspection (see, e.g., NCBI web site at ncbi.nlm.nih.gov/BLAST/ or the like). Such sequences are then said to be “substantially identical.” This definition also refers to, or may be applied to, the compliment of a test sequence. The definition also includes sequences that have deletions and/or additions, as well as those that have substitutions. As described below, the preferred algorithms can account for gaps and the like. Preferably, identity exists over a region that is at least about 25 amino acids or nucleotides in length, or more preferably over a region that is 50-100 amino acids or nucleotides in length. 
         [0025]    For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Preferably, default program parameters can be used, or alternative parameters can be designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters. 
         [0026]    A “comparison window,” as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 20 to 600, usually about 50 to about 200, more usually about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequences for comparison are well-known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith &amp; Waterman,  Adv. Appl. Math.  2:482 (1981), by the homology alignment algorithm of Needleman &amp; Wunsch,  J. Mol. Biol.  48:443 (1970), by the search for similarity method of Pearson &amp; Lipman,  Proc. Nat&#39;l. Acad. Sci. USA  85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by manual alignment and visual inspection (see, e.g.,  Current Protocols in Molecular Biology  (Ausubel et al., eds. 1987-2005, Wiley Interscience)). 
         [0027]    A preferred example of algorithm that is suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al.,  Nuc. Acids Res.  25:3389-3402 (1977) and Altschul et al.,  J. Mol. Biol.  215:403-410 (1990), respectively. BLAST and BLAST 2.0 are used, with the parameters described herein, to determine percent sequence identity for the nucleic acids and proteins of the invention. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always&gt;0) and N (penalty score for mismatching residues; always&lt;0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, M=5, N=−4 and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength of 3, and expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff &amp; Henikoff,  Proc. Natl. Acad. Sci. USA  89:10915 (1989)) alignments (B) of 50, expectation (E) of 10, M=5, N=−4, and a comparison of both strands. 
         [0028]    “Nucleic acid” refers to deoxyribonucleotides or ribonucleotides and polymers thereof in either single- or double-stranded form, and complements thereof The term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, which are synthetic, naturally occurring, and non-naturally occurring, which have similar binding properties as the reference nucleic acid, and which are metabolized in a manner similar to the reference nucleotides. Examples of such analogs include, without limitation, phosphorothioates, phosphoramidates, methyl phosphonates, chiral-methyl phosphonates, 2-O-methyl ribonucleotides, peptide-nucleic acids (PNAs). 
         [0029]    By “nucleic acid hybrid” or “probe:target duplex” is meant a structure that is a double-stranded, hydrogen-bonded structure, preferably about 10 to about 100 nucleotides in length, more preferably 14 to 50 nucleotides in length, although this will depend to an extent on the overall length of the oligonucleotide probe. The structure is sufficiently stable to be detected by means such as chemiluminescent or fluorescent light detection, autoradiography, electrochemical analysis or gel electrophoresis. Such hybrids include RNA:RNA, RNA:DNA, or DNA:DNA duplex molecules. 
         [0030]    “RNA and DNA equivalents” refer to RNA and DNA molecules having the same complementary base pair hybridization properties. RNA and DNA equivalents have different sugar groups (i.e., ribose versus deoxyribose), and may differ by the presence of uracil in RNA and thymine in DNA. The difference between RNA and DNA equivalents do not contribute to differences in substantially corresponding nucleic acid sequences because the equivalents have the same degree of complementarity to a particular sequence. 
         [0031]    By “preferentially hybridize” is meant that under high stringency hybridization conditions oligonucleotide probes can hybridize their target nucleic acids to form stable probe:target hybrids (thereby indicating the presence of the target nucleic acids) without forming stable probe:non-target hybrids (that would indicate the presence of non-target nucleic acids from other organisms). Thus, the probe hybridizes to target nucleic acid to a sufficiently greater extent than to non-target nucleic acid to enable one skilled in the art to accurately detect the presence of (for example  Candida ) and distinguish these species from other organisms. Preferential hybridization can be measured using techniques known in the art and described herein. 
         [0032]    By “theranostics” is meant the use of diagnostic testing to diagnose the disease, choose the correct treatment regime and monitor the patient response to therapy. The theranostics of the invention may be based on the use of an NAD assay of this invention on samples, swabs or specimens collected from the patient. 
       OBJECT OF THE INVENTION  
       [0033]    It is an object of the current invention to provide sequences and/or diagnostic assays to detect and identify one or more yeast and fungal species. The current inventors have made use of the eIF2γ gene sequence to design primers that are specific to  Candida  eIF2 γ genes and to  Aspergillus  eIF2γ genes. Such primers not only allow the detection of yeast and fungal species but also allow distinction between  Candida  and  Aspergillus  spp. This has an advantage over the prior art in that if one wants to identify a fungal pathogen in a sample, which contains  Candida  as a commensal, the approach of using universal primers may not be successful. There is a strong possibility that the  Candida  will out-compete the fungal pathogen in the amplification process and will be preferentially amplified, resulting in failure to detect the disease-causing pathogen. The current invention further provides for primers and probes that allow discrimination between different  Candida  species and among different  Aspergillus  species. 
       SUMMARY OF THE INVENTION 
       [0034]    The present invention provides for a diagnostic kit for detection and identification of yeast and/or fungal species, comprising at least one oligonucleotide probe capable of binding to at least a portion of the eIF2 γ gene or its corresponding mRNA. The oligonucleotide probe may have a sequence substantially homologous to or substantially complementary to a portion of the eIF2 γ gene or its corresponding mRNA. It will thus be capable of binding or hybridizing with a complementary DNA or RNA molecule. The eIF2 γ gene may be a fungal eIF2 γ gene. The eIF2 γ gene may be a yeast eIF2 γ gene. The nucleic acid molecule may be synthetic. 
         [0035]    The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 718 to 1040 of  C. albicans  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 790 to 934 of  C. albicans  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 872 to 972 of  C. glabrata  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 151 to 274 of  C. parapsilosis  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 140 to 270 of  C. tropicalis  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 115 to 224 of  C. krusei  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 121 to 374 of  A. fumigatus  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 164 to 261 of  A. fumigatus  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 155 to 252 of  A. flavus  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 92 to 189 of  A. niger  eIF2 γ gene. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 149 to 246 of  A. terreus  eIF2 γ gene. A skilled person will appreciate that sequences equivalent to these regions can be found in other organisms, but not necessarily in the same position. 
         [0036]    The oligonucleotide probe may have a sequence of SEQ ID NO 1, 2, 84, 85, 86, 87, 88, 108, 109, 110, 111, 125 or 138, or a sequence substantially homologous to or substantially complementary to those sequences, which can also act as a probe for the eIF2 γ gene. The kit may comprise more than one such probe. In particular the kit may comprise a plurality of such probes. In addition the kit may comprise additional probes for other organisms, such as, for example, bacterial species or viruses. 
         [0037]    The kit may further comprise at least one primer for amplification of at least a portion of the eIF2 γ gene. Suitably the kit may comprise at least one forward in vitro amplification primer and/ or at least one reverse in vitro amplification primer, the forward amplification primer having a sequence selected from the group comprising SEQ ID NO 3, 5, 89, 91, 93, 95, 97, 104, 105, 112, 113, 114, 115, 116, 117, 118, 119, 120, 135 or 136 or a sequence being substantially homologous or complementary thereto which can also act as a forward amplification primer for the eIF2 γ gene, and/or the reverse amplification primer having a sequence selected from the group comprising SEQ ID NO 4, 6, 90, 92, 94, 96, 98, 106, 107, 121, 122, 123, 124 or 137 or a sequence being substantially homologous or complementary thereto which can also act as a reverse amplification primer for the eIF2 γ gene. 
         [0038]    A kit useful for detecting a  Candida  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NOs: 1, 84, 85, 86, 87, 88 or 138 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NOs: 1, 84, 85, 86, 87, 88 or 138. The kit may further comprise at least one forward primer selected from the group comprising SEQ ID NOs: 5, 89, 91, 93, 95, 97, 135 or 136 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NOs: 5, 89, 91, 93, 95, 97, 135 or 136 and/or a reverse primer selected from the group comprising SEQ ID NOs: 6, 90, 92, 94, 96, 98, 137 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NOs: 6, 90, 92, 94, 96, 98 or 137. 
         [0039]    A kit for detecting or identifying a  Candida alicans  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 1, 84 or 138 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 1, 84 or 138 and further comprises at least one forward primer selected from the group comprising SEQ ID NO: 5, 89, 135 or 136 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 5, 89, 135 or 136 and/or at least one reverse primer selected from the group comprising SEQ ID NO: 6, 90 or 137.or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 6, 90, or 137. 
         [0040]    A kit for detecting or identifying a  Candida glabrata  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 85 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 85 and further comprises at least one forward primer selected from the group comprising SEQ ID NO: 91 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 91 and/or at least one reverse primer selected from the group comprising SEQ ID NO: 92 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 92. 
         [0041]    A kit for detecting or identifying a  Candida parapsilosis  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 86 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 86 and further comprises at least one forward primer selected from the group comprising SEQ ID NO: 93 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 93 and/or at least one reverse primer selected from the group comprising SEQ ID NO: 94. or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 94. 
         [0042]    A kit for detecting or identifying a  Candida tropicalis  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 87 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 87 and further comprises at least one forward primer selected from the group comprising SEQ ID NO: 95 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 95 and/or at least one reverse primer selected from the group comprising SEQ ID NO: 96 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 96. 
         [0043]    A kit for detecting or identifying a  Candida krusei  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 88 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 88 and further comprises at least one forward primer selected from the group comprising ID NO: 97 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 97 and/or at least one reverse primer selected from the group comprising SEQ ID NO: 98 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 98. 
         [0044]    A diagnostic kit for detecting or identifying an  Aspergillus  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NOs: 2, 108, 109, 110, 111 or 125 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NOs: 2, 108, 109, 110, 111 or 125. The kit may further comprise at least one forward primer selected from the group comprising SEQ ID NOs: 3, 104, 105, 112, 113, 114, 115, 116, 117, 118, 119 or 120 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NOs: 3, 104, 105, 112, 113, 114, 115, 116, 117, 118, 119 or 120 and/or at least one reverse primer selected from the group comprising SEQ ID NOs: 4, 106, 107, 121, 122, 123 or 124 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NOs: 4, 106, 107, 121, 122, 123 or 124. 
         [0045]    A kit for detecting or identifying a  Aspergillus fumigatus  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 2, 111 or 125 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 2, 111 or 125 and further comprises at least one forward primer selected from the group comprising SEQ ID NO: 3, 112, 113, 115, 117, 119, or 120 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 3, 112, 113, 115, 117, 119, or 120 and/or at least one reverse primer selected from the group comprising SEQ ID NO: 4, 106, 121, 123, 124 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 4 ,106, 121, 123, 124. 
         [0046]    A kit for detecting or identifying a  Aspergillus flavus  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 110 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 110 and further comprises at least one forward primer selected from the group comprising SEQ ID NO: 104 or 114 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 104 or 114 and at least one reverse primer selected from the group comprising SEQ ID NO: 106 122, or 123 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 106 122, or 123 
         [0047]    A kit for detecting or identifying a  Aspergillus niger  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 108 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 108 and further comprises at least one forward primer selected from the group comprising SEQ ID NO: 104, 114, 116 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 104, 114, 116 and at least one reverse primer selected from the group comprising SEQ ID NO: 107, 122, 123 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 107, 122, 123. 
         [0048]    A kit for detecting or identifying a  Aspergillus terreus  eIF2γ polynucleotide comprises at least one oligonucleotide probe selected from the group comprising SEQ ID NO: 109 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 109 and further comprises at least one forward primer selected from the group comprising SEQ ID NO: 105, 115, 118 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 105, 115, 118 and at least one reverse primer selected from the group comprising SEQ ID NO: 107, 122 or 123 or which preferentially hybridizes to the same nucleotide sequence as is preferentially hybridized by SEQ ID NO: 107, 122 or 123. 
         [0049]    The identified sequences are suitable not only for in vitro DNA/RNA amplification based detection systems but also for signal amplification based detection systems. Furthermore, the sequences of the invention identified as suitable targets provide the advantages of having significant intragenic sequence heterogeneity in some regions, which is advantageous and enables aspects of the invention to be directed towards group or species-specific targets, and also having significant sequence homogeneity in some regions, which enables aspects of the invention to be directed towards genus-specific yeast and fungal primers and probes for use in direct nucleic acid detection technologies, signal amplification nucleic acid detection technologies, and nucleic acid in vitro amplification technologies for yeast and fungal diagnostics. The eIF2 γ sequences allow for multi-test capability and automation in diagnostic assays. 
         [0050]    One of the advantages of the sequences of the present invention is that the intragenic eIF2 γ nucleotide sequence diversity between closely related yeast and fungal species enables specific primers and probes for use in diagnostics assays for the detection of yeast and fungi to be designed. The eIF2 γ nucleotide sequences, both DNA and RNA can be used with direct detection, signal amplification detection and in vitro amplification technologies in diagnostics assays. The eIF2 γ sequences allow for multi-test capability and automation in diagnostic assays. 
         [0051]    The kit may further comprise at least one primer for amplification of at least a portion of the eIF2 γ gene. Suitably the kit comprises a forward and a reverse primer for a portion of the eIF2 γ gene. 
         [0052]    The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 718 to base pair position 1040 of the eIF2 γ gene in  C. albicans.  Particularly preferred are kits comprising a probe for a portion of the eIF2  γ C. albicans  gene and/or a probe for a portion of the region of the gene equivalent to base pair position 718 to base pair position 1040 of the eIF2 γ gene in  C. albicans.  Sequences equivalent to base pair position 718 to base pair position 1040 can be found in other organisms, but not necessarily in the same position. The portion of the eIF2 γ gene may be equivalent to a portion of the region of the gene from base pair position 121 to base pair position 374 in  Aspergillus fumigatus.  Particularly preferred, are kits comprising a probe for a portion of the eIF2  γ A. fumigatus  gene and/or a probe for a portion of the region of the gene equivalent to base pair position 121 to base pair position 374 in  Aspergillus fumigatus.  Sequences equivalent to base pair position 121 to base pair position 374 can be found in other organisms, but not necessarily in the same position. The kit may also comprise additional primers or probes. The primer may have a sequence selected from the group SEQ ID NO 3 through to SEQ ID NO 6 or a sequence substantially homologous to or substantially complementary to those sequences, which can also act as a primer for the eIF2 γ gene. 
         [0053]    The kit may comprise at least one forward in vitro amplification primer and at least one reverse in vitro amplification primer, the forward amplification primer having a sequence selected from the group consisting of SEQ ID NO 3 or SEQ ID NO 5.or a sequence being substantially homologous or complementary thereto which can also act as a forward amplification primer for the eIF2 γ gene, and the reverse amplification primer having a sequence selected from the group consisting of SEQ ID NO 4 or SEQ ID NO 6 or a sequence being substantially homologous or complementary thereto which can also act as a reverse amplification primer for the eIF2 γ gene. The diagnostic kit may be based on direct nucleic acid detection technologies, signal amplification nucleic acid detection technologies, and nucleic acid in vitro amplification technologies is selected from one or more of Polymerase Chain Reaction (PCR), Ligase Chain Reaction (LCR), Nucleic Acids Sequence Based Amplification (NASBA), Strand Displacement Amplification (SDA), Transcription Mediated Amplification (TMA), Branched DNA technology (bDNA) and Rolling Circle Amplification Technology (RCAT)), or other in vitro enzymatic amplification technologies. 
         [0054]    The invention also provides a nucleic acid molecule selected from the group consisting of SEQ ID NO.1 to SEQ ID NO. 135, preferably SEQ ID NO. 1 through to 74 and SEQ ID NO. 84 through to 135, and sequences substantially homologous thereto, or substantially complementary to a portion thereof and having a function in diagnostics based on the eIF2 y gene. The nucleic acid molecule may comprise an oligonucleotide having a sequence substantially homologous to or substantially complementary to a portion of a nucleic acid molecule of SEQ ID NO.1 to SEQ ID NO. 135, preferably SEQ ID NO. 1 through to 74 and SEQ ID NO. 84 through to 135. The invention also provides a method of detecting a target organism in a test sample comprising the steps of:
       (i) mixing the test sample with at least one oligonucleotide probe as defined above under appropriate conditions; and   (ii) hybridizing under high stringency conditions any nucleic acid that may be present in the test sample with the oligonucleotide to form a probe:target duplex; and   (iii) determining whether a probe:target duplex is present; the presence of the duplex positively identifying the presence of the target organism in the test sample.       
 
         [0058]    The nucleic acid molecule and kits of the present invention may be used in a diagnostic assay to detect the presence of one or more yeast and/or fungal species, to measure yeast and/or fungal titres in a patient or in a method of assessing the efficacy of a treatment regime designed to reduce yeast and/or fungal titre in a patient or to measure yeast and/or fungal contamination in an environment. The environment may be a hospital, or it may be a food sample, an environmental sample e.g. water, an industrial sample such as an in-process sample or an end product requiring bioburden or quality assessment. 
         [0059]    The kits and the nucleic acid molecule of the invention may be used in the identification and/or characterization of one or more disruptive agents that can be used to disrupt the eIF2 γ gene function. The disruptive agent may be selected from the group consisting of antisense RNA, PNA, and siRNA. 
         [0060]    In some embodiments of the invention, a nucleic acid molecule comprising a species-specific probe can be used to discriminate between species of the same genus. 
         [0061]    The oligonucleotides of the invention may be provided in a composition for detecting the nucleic acids of yeast and fungal target organisms. Such a composition may also comprise buffers, enzymes, detergents, salts and so on, as appropriate to the intended use of the compositions. It is also envisioned that the compositions, kits and methods of the invention, while described herein as comprising at least one synthetic oligonucleotide, may also comprise natural oligonucleotides with substantially the same sequences as the synthetic nucleotide fragments in place of, or alongside synthetic oligonucleotides. 
         [0062]    The invention also provides for an in vitro amplification diagnostic kit for a target yeast and/or fungal organism comprising at least one forward in vitro amplification primer and at least one reverse in vitro amplification primer, the forward amplification primer being selected from the group consisting of one or more of a sequence being substantially homologous or complementary thereto which can also act as a forward amplification primer, and the reverse amplification primer being selected from the group consisting of one or more of or a sequence being substantially homologous or complementary thereto which can also act as a reverse amplification primer. 
         [0063]    The invention also provides for a diagnostic kit for detecting the presence of candidate yeast and/or fungal species, comprising one or more DNA probes comprising a sequence substantially complementary to, or substantially homologous to the sequence of the eIF2 γ gene of the candidate yeast and/or fungal species. The present invention also provides for one or more synthetic oligonucleotides having a nucleotide sequence substantially homologous to or substantially complementary to one or more of the group consisting of the eIF2 γ gene or mRNA transcript thereof, the yeast and or fungal eIF2 γ gene or mRNA transcript thereof, the yeast eIF2 γ gene or mRNA transcript thereof, one or more of SEQ ID NO 1-SEQ ID NO 135, preferably SEQ ID NO. 1 through to 74 and SEQ ID NO. 84 through to 135. 
         [0064]    The nucleotide may comprise DNA. The nucleotide may comprise RNA. The nucleotide may comprise a mixture of DNA, RNA and PNA. The nucleotide may comprise synthetic nucleotides. The sequences of the invention (and the sequences relating to the methods, kits compositions and assays of the invention) may be selected to be substantially homologous to a portion of the coding region of the eIF2 γ gene. The gene may be a gene from a target yeast or fungal organism. The sequences of the invention are preferably sufficient so as to be able form a probe:target duplex to the portion of the sequence. 
         [0065]    The invention also provides for a diagnostic kit for a target yeast or fungal organism comprising an oligonucleotide probe substantially homologous to or substantially complementary to an oligonucleotide of the invention (which may be synthetic). It will be appreciated that sequences suitable for use as in vitro amplification primers may also be suitable for use as oligonucleotide probes: while it is preferable that amplification primers may have a complementary portion of between about 15 nucleotides and about 30 nucleotides (more preferably about 15-about 23, most preferably about 20 to about 23), oligonucleotide probes of the invention may be any suitable length. The skilled person will appreciate that different hybridization and or annealing conditions will be required depending on the length, nature &amp; structure (eg. Hybridization probe pairs for LightCycler, Taqman 5′ exonuclease probes, hairpin loop structures etc. and sequence of the oligonucleotide probe selected. 
         [0066]    Kits and assays of the invention may also be provided wherein the oligonucleotide probe is immobilized on a surface. Such a surface may be a bead, a membrane, a column, dipstick, a nanoparticle, the interior surface of a reaction chamber such as the well of a diagnostic plate or inside of a reaction tube, capillary or vessel or the like. 
         [0067]    The target yeast or fungal organism may be selected from the group consisting of  C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis, C. dubliniensis, C. guilliermondii, C. norvegiensis, C. famata, C. haemuloni, C. kefyr, C. utilis, C. viswanathii, C. lusitaniae  and  C. cifferii, A. fumigatus, N. fischeri, A. clavatus, A. niger, A. terreus, A. flavus, A. versicolor  and  A. nidulans.    
         [0068]    The target yeast organisms may be a  Candida  species for the given set of primers already experimentally demonstrated, and more preferably, selected from the group consisting of  C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. dubliniensis  and  C. tropicalis, C. guilliermondii, C. norvegiensis, C. famata, C. haemuloni, C. kefyr, C. utilis, C. viswanathii, C. lusitaniae  and  C. cifferii.    
         [0069]    Under these circumstances, the amplification primers and oligonucleotide probes of the invention may be designed to a gene specific or genus specific region so as to be able to identify one or more, or most, or substantially all of the desired organisms of the target yeast organism grouping. 
         [0070]    The target fungal organisms may be an  Aspergillus  species for given set of primers already experimentally demonstrated, and more preferably, selected from the group consisting of  A. fumigatus, N. fischeri, A. clavatus, A. niger, A. terreus, A. flavus, A. versicolor  and  A. nidulans.    
         [0071]    The test sample may comprise cells of the target yeast and/or fungal organism. The method may also comprise a step for releasing nucleic acid from any cells of the target yeast or fungal organism that may be present in said test sample. Ideally, the test sample is a lysate of an obtained sample from a patient (such as a swab, or blood, urine, saliva, a bronchial lavage dental specimen, skin specimen, scalp specimen, transplant organ biopsy, stool, mucus, or discharge sample). The test samples may be a food sample, a water sample an environmental sample, an end product, end product or in-process industrial sample. 
         [0072]    The invention also provides for the use of any one of SEQ ID NO.1 to SEQ ID NO. 135, preferably SEQ ID NO. 1 through to 74 and SEQ ID NO. 84 through to 135, in a diagnostic assay for the presence of one or more yeast or fungal species. The species may be selected from the group consisting of  C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis, C. dubliniensis, C. guilliermondii, C. norvegiensis, C. famata, C. haemuloni, C. kefyr, C. utilis, C. viswanathii, C. lusitaniae, C. cifferii, A. fumigatus, N. fischeri, A. clavatus, A. niger, A. terreus, A. flavus, A. versicolor  and  A. nidulans.    
         [0073]    The invention also provides for kits for use in theranostics, food safety diagnostics, industrial microbiology diagnostics, environmental monitoring, veterinary diagnostics, bio-terrorism diagnostics comprising one or more of the synthetic oligonucleotides of the invention. The kits may also comprise one or more articles selected from the group consisting of appropriate sample collecting instruments, reagent containers, buffers, labelling moieties, solutions, detergents and supplementary solutions. The invention also provides for use of the sequences, compositions, nucleotide fragments, assays, and kits of the invention in clinical diagnostics, theranostics, Food safety diagnostics, Industrial microbiology diagnostics, Environmental monitoring, Veterinary diagnostics, Bio-terrorism diagnostics. 
         [0074]    The nucleic acid molecules, composition, kits or methods may be used in a diagnostic nucleic acid based assay for the detection of yeast and/or fungal species. 
         [0075]    The nucleic acid molecules, composition, kits or methods may be used in a diagnostic assay to measure yeast and/or fungal titres in a patient. The titres may be measured in vitro. 
         [0076]    The nucleic acid molecules, composition, kits or methods may be used in a method of assessing the efficacy of a treatment regime designed to reduce yeast and/or fungal titre in a patient comprising assessing the yeast and/or fungal titre in the patient (by in vivo methods or in vitro methods) at one or more key stages of the treatment regime. Suitable key stages may include before treatment, during treatment and after treatment. The treatment regime may comprise an antifungal agent, such as a pharmaceutical drug. 
         [0077]    The nucleic acid molecules, composition, kits or methods may be used in a diagnostic assay to measure potential yeast and/or fungal contamination, for example, in a hospital. 
         [0078]    The nucleic acid molecules, composition, kits or methods may be used in the identification and/or characterization of one or more disruptive agents that can be used to disrupt the eIF2 γ gene function. Suitable disruptive agents may be selected from the group consisting of antisense RNA, PNA, siRNA. 
         [0079]    The current invention will now be described with reference to the following figures. It is to be understood that the following detailed description and accompanying figures, are exemplary and explanatory only and are intended to provide a further explanation of the present invention, as claimed and not to limit the scope of the invention in any way. 
     
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         [0080]      FIG. 1 : Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Candida albicans  (XM — 715569.1). The amplified region of interest is underlined. (Position of the region of interest: 718-1040). 
           [0081]      FIG. 2 : Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Aspergillus fumigatus  (XM — 746974.2). The amplified region of interest is underlined. (Position of the region of interest: 121-374). 
           [0082]      FIG. 3 : Amplification plot from Real-time PCR assay for  C. albicans  based on the eIF2 γ gene with TaqMan probe P1-CaneIF2. Specificity of the assay was tested using a panel of DNA from 4 other  Candida  species and  Aspergillus fumigatus.  The 3  C. albicans  strains tested were detected and no cross-reaction was seen with DNA from the other  Candida  species and  A. fumigatus.    
           [0083]      FIG. 4 : Amplification plot from Real-time PCR assay for  A. fumigatus  based on the eIF2 γ gene with TaqMan probe P1-AspeIF2. Specificity of the assay was tested against a panel of DNA from 6 closely related  Aspergillus  species and  C. albicans.  The 3  A. fumigatus  strains were detected and no cross-reaction was seen with DNA from the other  Aspergillus  spp and  C. albicans.    
           [0084]      FIG. 5 : (SEQ ID NO: 99) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Candida albicans  (XM — 715569.1). The amplified region of interest is underlined. (Position of the region of interest: 664-1040). 
           [0085]      FIG. 6 : (SEQ ID NO: 100) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Candida glabrata  (XM — 447610.1). The amplified region of interest is underlined. (Position of the region of interest: 872-972) 
           [0086]      FIG. 7 : (SEQ ID NO: 101) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Candida parapsilosis  (CBS 604 generated sequence). The amplified region of interest is underlined. (Position of the region of interest: 151-274). 
           [0087]      FIG. 8 : (SEQ ID NO: 102) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Candida tropicalis  (CBS 94 generated sequence). The amplified region of interest is underlined. (Position of the region of interest: 140-270) 
           [0088]      FIG. 9 : (SEQ ID NO: 103) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Candida krusei  (CBS 573 generated sequence). The amplified region of interest is underlined. (Position of the region of interest: 115-224) 
           [0089]      FIG. 10 : Amplification plot from Real-time PCR assay for  C. albicans  based on the eIF2 γ gene with TaqMan probe ALEF2. Specificity of the assay was tested using a panel of DNA from 14  C. albicans  strains and 19 other  Candida  species. (a) The 14  C. albicans  strains tested were detected. (b) No cross-reaction was seen with DNA from 19 the other  Candida  species. Signal obtained only from (+) control. (c) Sensitivity of the assay was tested using a various inputs of template DNA from  C. albicans.  The LOD of the assay was found to be between 1-5 cell equivalents. 
           [0090]      FIG. 11 : Amplification plot from Real-time PCR assay for  C. glabrata  based on the eIF2γ gene with TaqMan probe GlabA. Specificity of the assay was tested using a panel of DNA from 10  C. glabrata  strains, 19 other  Candida  species,  Aspergillus fumigatus  and  Saccharomyces cerevisiae.  (a) The 10  C. glabrata  strains tested were detected. (b) No cross-reaction was seen with DNA from 19 the other  Candida  species or with  Aspergillus fumigatus  or  Saccharomyces cerevisiae.  Signal obtained only from (+) control. (c) The LOD of the assay was found to be ˜2 cell equivalents. 
           [0091]      FIG. 12 : Amplification plot from Real-time PCR assay for  C. parapsilosis  based on the eIF2 γ gene with TaqMan probe ParA. Specificity of the assay was tested using a panel of DNA from 12  C. parapsilosis  strains, 19 other  Candida  species,  Aspergillus fumigatus  and  Saccharomyces cerevisiae.  (a) The 12  C. parapsilosis  strains tested were detected. (b) No cross-reaction was seen with DNA from the other 19  Candida  species or with  Aspergillus fumigatus  or  Saccharomyces cerevisiae.  Signal obtained only from (+) control. (c) The LOD of the assay was found to be ˜10 cell equivalents. 
           [0092]      FIG. 13 : Amplification plot from Real-time PCR assay for  C. tropicalis  based on the eIF2 γ gene with TaqMan probe TropicA. Specificity of the assay was tested using a panel of DNA from 12  C. tropicalis  strains, 19 other  Candida  species,  Aspergillus fumigatus  and  Saccharomyces cerevisiae.  (a) The 12  C. tropicalis  strains tested were detected. (b) No cross-reaction was seen with DNA from the other 19  Candida  species or with  Aspergillus fumigatus  or  Saccharomyces cerevisiae.  Signal obtained only from (+) control. (c) The LOD of the assay was found to be ˜20 cell equivalents. 
           [0093]      FIG. 14 : Amplification plot from Real-time PCR assay for  C. krusei  based on the eIF2 gene with TaqMan probe KrusA. Specificity of the assay was tested using a panel of DNA from 9  C. krusei  strains, 19 other  Candida  species,  Aspergillus fumigatus  and  Saccharomyces cerevisiae.  (a) The 9  C. krusei  strains tested were detected. (b) No cross-reaction was seen with DNA from the other 19  Candida  species or with  Aspergillus fumigatus  or  Saccharomyces cerevisiae.  Signal obtained only from (+) control. (c) The LOD of the assay was found to be ˜2 cell equivalents. 
           [0094]      FIG. 15 : (SEQ ID NO: 50) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Aspergillus fumigatus  (7273 generated sequence). The amplified region of interest is underlined. (corresponding to base pair positions 164-261 of published sequence). 
           [0095]      FIG. 16 : (SEQ ID NO: 122) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Aspergillus flavus  (117.62 generated sequence). The amplified region of interest is underlined. (corresponding to base pair positions 155-252 of published sequence). 
           [0096]      FIG. 17 : (SEQ ID NO: 58) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Aspergillus niger  (6727 generated sequence). The amplified region of interest is underlined (corresponding to base pair positions 92-189 of published sequence). 
           [0097]      FIG. 18 : (SEQ ID NO: 64) Primers binding sites (grey highlights) and probe (bold text) in eIF2γ of  Aspergillus terreus  (5677 generated sequence). The amplified region of interest is underlined. (corresponding to base pair positions 149-246 of published). 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0098]    Materials and Methods 
         [0099]    Cell Culture 
         [0100]    A collection of geographically distinct strains of clinically relevant  Candida  species was obtained from a number of culture collections.  Candida  species were cultured in Sabouraud broth (4% wt/vol glucose, 1% wt/vol peptone, 1.5% agar) for 48 hours at 37° C. in a shaking incubator.  Aspergillus  species ( A. fumigatus, A. flavus, A. niger  and  A. terruous  and other closely related species) were cultured in Sabouraud broth (4% wt/vol glucose, 1% wt/vol peptone, 1.5% agar) or agar for 3-4 days at 25° C. 
         [0101]    DNA Extraction 
         [0102]    Cells from  Candida  and  Aspergillus  spp. were pretreated with lyticase or zymolase enzymes prior to DNA isolation. DNA was isolated from  Candida  and  Apergillus  spp. using the MagNA Pure System (Roche Molecular Systems) in combination with the MagNA pure Yeast and Bacterial isolation kit III or with the Qiagen Plant kit-according to manufacturers instructions. 
         [0103]    Sequencing of eIF2 γ Gene of  Candida  and  Aspergillus  Species 
         [0104]    The publicly available sequences of the eIF2 genes of  Candida  or  Aspergillus  species were acquired from the NCBI database and aligned using Clustal W. 
         [0105]    The PCR primer set CaneIF2-F/CaneIF2-R was designed to amplify the eIF2γ gene region in  Candida  spp. equivalent to nucleotide position 718 to nucleotide position 1040 in  C. albicans  (XM — 715569.1). (Table 1,  FIG. 1 ). The PCR primer set AspeIF2-F/AspeIF2-R was designed to amplify a region of the eIF2γ gene in  Aspergillus  species equivalent to nucleotide position 121 to nucleotide position 374 in  A. fumigatus (XM — 746974.2). (Table 1). The eIF2 gene regions were amplified in a range of  Candida  and  Aspergillus  spp. by conventional PCR on the iCycler BioRad PCR machine or the PTC200 Peltier thermocycler (MJ Research) using the reagents outlined in Table 2 and the thermocycling conditions described in Table 3. In order to generate sequence information, a total of 72 strains representing 20  Candida  species were tested for amplification with this primer set by conventional PCR on the iCycler BioRad PCR machine. PCR products were generated for 15  Candida  species.  C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis, C. dubliniensis, C. guilliermondii, C. norvegiensis, C. famata, C. haemuloni, C. kefyr, C. utilis, C. viswanathii, C. lusitaniae  and  C. cifferii.  and 7  Aspergillus  species ( A. fumigatus, A. clavatus, A. niger, A. terreus, A. flavus, A. versicolor, A. nidulans ) and  Neosartorya fischeri ). The PCR reaction products were purified with Roche High Pure PCR Product Purification kit or with the ExoSAP-IT kit (USB) according to the manufacturers&#39; instructions and subsequently sequenced by Sequiserve using the forward amplification primer CaneIF2-F or AspeIF2-F. 
         [0106]    DNA sequence information was generated for 15  Candida  species. ( C. albicans, C. glabrata, C. krusei, C. parapsilosis, C. tropicalis, C. dubliniensis, C. guilliermondii, C. norvegiensis, C. famata, C. haemuloni, C. kefyr, C. utilis, C. viswanathii, C. lusitaniae  and  C. cifferii,  and 7  Aspergillus  species ( A. fumigatus, A. clavatus, A. niger, A. terreus, A. flavus, A. versicolor, A. nidulans ) and  Neosartorya fischeri.    
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 PCR primers designed to amplify the eIF2 γ 
               
               
                 gene regions in  Candida  and  Aspergillus  spp 
               
             
          
           
               
                 Primer Name 
                 Primer Sequence 
               
               
                   
               
               
                 AspeIF2-F 
                 CTTAAGTCTGCGATGAAGA 
               
               
                   
               
               
                 AspeIF2-R 
                 GTAATGTTACGCTCCAACTC 
               
               
                   
               
               
                 CaneIF2-F 
                 GCTGCCATTGAAATTATGAA 
               
               
                   
               
               
                 CaneIF2-R 
                 GAACCACCTGCAACACC 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 PCR reagents used to amplify the eIF2 γ gene regions 
               
               
                 in  Candida  and  Aspergillus  spp. 
               
             
          
           
               
                   
                   
                 SAMPLE 
               
               
                   
                 PCR Reaction Mix 
                 x 1 
               
               
                   
                   
               
             
          
           
               
                   
                 10 x Buffer (100 mM Tris HCl, 15 mM MgCl 2 , 
                 5 
                 μl 
               
               
                   
                 500 mM KCl pH 8.3) 
               
               
                   
                 dNTP&#39;s Mix, Roche (10 mM dNTP) 
                 1 
                 μl 
               
               
                   
                 Primer Forward CaneIF2-F or AspeIF2-F (10 μM) 
                 1 
                 μl 
               
               
                   
                 Primer Reverse CaneIF2-R or AspeIF2-R (10 μM) 
                 1 
                 μl 
               
               
                   
                 Polymerase TaqPol, Roche 1 U/μl 
                 1 
                 μl 
               
               
                   
                 H20 Amgen/Accugene 
                 36-39 
                 μl 
               
               
                   
                 DNA Template 
                 2-5 
                 μl 
               
               
                   
                 TOTAL VOLUME 
                 50 
                 μl 
               
               
                   
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 PCR thermocycling conditions used to amplify eIF2 γ gene 
               
               
                 regions in  C. albicans  and  A. fumigatus.   
               
               
                 PCR Thermal profile  Lid preheating was ON 
               
             
          
           
               
                   
                 Step 
                 Temp 
                 Time 
               
               
                   
                   
               
               
                   
                 1 
                 94° C. 
                 1 min 
                 X 35 
               
               
                   
                 2 
                 50° C., 51° C. 
                 1 min 
               
               
                   
                 3 
                 72° C. 
                 1 min 
               
               
                   
                 4 
                 72° C. 
                 7 min 
               
               
                   
                 5 
                  8° C. 
                 Hold 
               
               
                   
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 TaqMan probes (with 5′-FAM and 3&#39;-BHQ1) based on 
               
               
                 eIF2 γ gene for  C. albicans  and  A. fumigatus . 
               
             
          
           
               
                 Probe Name  
                 Probe Sequence 
               
               
                   
               
               
                 P1-CaneIF2 
                 CGATAATGCTCCGATCGTGCCTA 
               
               
                   
               
               
                 P1-AspeIF2 
                 CGCTCACACCTCTGTCGCCCGAA 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Real-time PCR reagents 
               
             
          
           
               
                   
                 SAMPLE 
               
               
                 Preparation of PCR Reaction Mix 
                 X 1 
               
               
                   
               
               
                 HybProb mix 10 x conc. (LightCycler ® 
                 2 μl 
               
               
                 FastStartDNA Master HybProbe Kit) 
               
               
                 MgCl 2  stock solution (Final conc. in reaction is 3 mM) 
                 1.6 μl   
               
               
                 Probe P1-CaneIF2 or P1-AspeIF2 
                 2 μl 
               
               
                 Primer Forward CaneIF2-F or AspeIF2-F 
                 1 μl 
               
               
                 Primer Reverse CaneIF2-R or AspeIF2-R 
                 1 μl 
               
               
                 H 2 O PCR-grade 
                 10.4 μl   
               
               
                 Template 
                 2 μl 
               
               
                 TOTAL VOLUME 
                 20 μl  
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 Real-time PCR thermocycling conditions 
               
               
                 PCR Thermal profile 
               
             
          
           
               
                   
                 Cycle 
                 Step 
                 Temp 
                 Time 
                   
               
               
                   
                   
               
             
          
           
               
                   
                 Activation 
                 1 
                 95° 
                 C. 
                 10 min 
                 X 50 
               
               
                   
                 Amplification 
                 1 
                 95° 
                 C. 
                 10 sec 
               
               
                   
                   
                 2 
                 62 or 65° 
                 C. 
                 20 sec 
               
               
                   
                   
                 3 
                 70° 
                 C. 
                 10 sec 
               
               
                   
                 Cooling 
                 1 
                 40° 
                 C. 
                 Hold 
               
               
                   
                   
               
               
                   
                 The PCR was performed with LightCycler ® Roche 
               
             
          
         
       
     
         [0107]    Results 
         [0108]    Primer and Probe Design 
         [0109]    The publicly available sequences for the eIF2γ gene in  Candida  spp. was aligned with the newly generated sequence information for the eIF2γ gene in  Candida  spp. and analyzed using bioinformatics tools. The publicly available sequence information for the eIF2γ gene in  Aspergillus  spp. was aligned with the newly generated sequence information for the eIF2γ gene in  Aspergillus  spp. and analyzed using bioinformatics tools. Species-specific probes were designed based on the compiled eIF2γ sequence information for  Candida albicans  and  Aspergillus fumigatus  (Table 4). 
         [0110]    Real-Time PCR 
         [0111]      FIGS. 1-2  show the relative positions of the PCR primers and TaqMan DNA probes for the amplification and detection of  C. albicans  and  A. fumigatus.  The specificity of the TaqMan probes for the identification of  C. albicans  and  A. fumigatus  was demonstrated in real-time PCR assays on the LightCycler using the reagents and thermocycling conditions outlined in Tables 5 and 6. For the  C. albicans  assay based on the eIF2γ gene, PCR primers CaneIF2-F/CaneIF2-R were combined with TaqMan probe, P 1-CaneIF2. The specificity of the assay for the detection of  C. albicans  was confirmed by including DNA from a range of closely related  Candida  species and  A. fumigatus  in the  C. albicans  real-time PCR assay. The assay detected three  C. albicans  strains tested but did not detect or cross-react with DNA from any other  Candida  species tested or with  A. fumigatus  DNA.  FIG. 3  shows the  C. albicans  real-time PCR assay and the specificity of the assay for  C. albicans.    
         [0112]    For the  A. fumigatus  assay based on the eIF2γ gene PCR primers AspeIF2-F/AspeIF2-R were combined with TaqMan probe, P1-AspeIF2. The specificity of the assay for the detection of  A. fumigatus  was confirmed by including DNA from a range of closely related  Aspergillus  species and  C. albicans  in the  A. fumigatus  real-time PCR assay. The assay detected  A. fumigatus  but did not detect or cross-react with DNA from  C. albicans  or any other  Aspergillus  species tested.  FIG. 4  shows the  A. fumigatus  real-time PCR assay and the specificity of the assay for  A. fumigatus.    
         [0113]      Candida  spp 
         [0114]    Primer and Probe Design 
         [0115]    The publicly available sequences for the eIF2γ gene in  Candida  spp. were aligned with the newly generated sequence information for the eIF2γ gene in  Candida  spp. and analysed using bioinformatics tools. Species-specific probes were designed based on the compiled eIF2γ sequence information for  Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis  and  Candida parapsilosis  (Table 7 and 8).  FIGS. 5-9  show the relative positions of the PCR primers and TaqMan DNA probes for the amplification and detection of  Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis  and  Candida parapsilosis.    
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 7 
               
               
                   
               
               
                 Additional primer and probes designed and tested 
               
               
                 for use in  C. albicans  specific assay 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 Primer Name 
                 Primer Sequence 5&#39;-&gt;3&#39; 
               
               
                   
               
               
                 CEF1F 
                 5&#39;-ATCTATCATTCAGTTTATTAGAG-3&#39; 
               
               
                   
               
               
                 CEF2F 
                 5&#39;-CATTCAGTTTATTAGAGGTAC-3&#39; 
               
               
                   
               
               
                 CEFR2 
                 5&#39;-CAGTAAAGTCTCTCATTG-3&#39; 
               
               
                   
               
               
                 Probe Name 
                 Probe Sequence 5&#39;-&gt;3&#39; 
               
               
                   
               
               
                 ALEF1 
                 FAM-TGCCGATAATGCTCCGATC-BHQ1 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 TaqMan probes (with 5&#39;-FAM and 3&#39;-BHQ1) based on 
               
               
                 eIF2γ gene for  C. albicans ,  C. glabrata ,  C .  
               
               
                   parapsilosis ,  C. tropicalis , and  C. krusei . 
               
             
          
           
               
                 Probe Name 
                 Probe Sequence 5&#39;-&gt;3&#39; 
               
               
                   
               
               
                 ALEF2 
                 6FAM-ATAATGCTCCGATCGTGCCTA-BHQ1 
               
               
                   
               
               
                 GlabA 
                 6FAM-CAAGAGATTTCATGCTTTCTCCAC-BHQ1 
               
               
                   
               
               
                 ParA 
                 6FAM-CGTAAACTCAATACCAGTTCCAGTC-BHQ1 
               
               
                   
               
               
                 TropicA 
                 6FAM-TGTCAATTATATCCCAGTTCCATTGA-BHQ1 
               
               
                   
               
               
                 KrusA 
                 6FAM-CATGTGTATGGTCAAGTCTATTCCT-BHQ1 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 Real Time PCR primers based on eIF2 gene for 
               
               
                   C .  albicans ,  C .  glabrata ,  C .  parapsilosis , 
               
               
                   C .  tropicalis , and  C .  krusei . 
               
             
          
           
               
                 Primer Name 
                 Primer Sequence 5&#39;-&gt;3&#39; 
               
               
                   
               
               
                 CEF3F 
                 5&#39;-TCAGCCTTGGAACAC-3&#39; 
               
               
                   
               
               
                 CEFR1 
                 5&#39;-TTGGCACAGGTATGTAG-3&#39; 
               
               
                   
               
               
                 GlabF1 
                 5&#39;-TCgTgAAgACTATCCCTgT-3&#39; 
               
               
                   
               
               
                 GlabR1 
                 5&#39;-ATCGATTTCAGCACCTGG-3&#39; 
               
               
                   
               
               
                 ParaF1 
                 5&#39;-TATCgACgCCgTCAATC-3&#39; 
               
               
                   
               
               
                 ParaR1 
                 5&#39;-ATCAACgTCAgCACCAg-3&#39; 
               
               
                   
               
               
                 TropicF1 
                 5&#39;-ACATCGATGCCGTTAACC-3&#39; 
               
               
                   
               
               
                 TropicR1 
                 5&#39;-CAAGTCTTCGACATCGGA-3&#39; 
               
               
                   
               
               
                 KrusF1 
                 5&#39;-CCCAATTTCTGCTCAGTTG-3&#39; 
               
               
                   
               
               
                 KrusR1 
                 5&#39;-CACCAGGCTTATTAACATCG-3&#39; 
               
               
                   
               
             
          
         
       
     
         [0116]    Real Time PCR 
         [0117]    The specificity of the TaqMan probes for the identification of  Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis  and  Candida parapsilosis  was demonstrated in real-time PCR assays on the LightCycler using the thermocycling conditions outlined in Table 10 (a) &amp; (b) ( C. albicans ). 
         [0000]    
       
         
               
             
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 Initial amplification conditions for evaluation of  C. glabtrata , 
               
               
                   C. parapsilosis ,  C. krusei  and  C. tropicalis  assay performance. 
               
             
          
           
               
                   
                 Amplification Protocol (b) 
               
               
                 Amplification Protocol (a) 
                 PCR conditions: 
               
               
                 PCR conditions: Mn2+ (Z05) 
                 Mn2+ (Z05) 
               
               
                   
               
             
          
           
               
                 50° C. 
                  2 min 
                 UNG 
                 50° C. 
                  2 min 
                 UNG 
               
               
                 95° C. 
                  1 min 
                   
                 95° C. 
                  1 min 
               
               
                 95° C. 
                 10 sec 
                 45 cycles 
                 95° C. 
                  1 sec 
                 45 cycles 
               
               
                 60° C. 
                 30 sec 
                   
                 60° C. 
                 10 sec 
               
               
                 40° C. 
                  2 min 
                  1 cycle 
                 40° C. 
                  2 min 
                  1 cycle 
               
               
                   
               
             
          
         
       
     
         [0118]    For the  C. albicans  assay based on the eIF2γ gene, following evaluation of the primers and probes listed in Table 7 and 8, PCR primers CEF3F/CEFR1 were combined with TaqMan probe, ALEF2. The specificity of the assay for the detection of  C. albicans  was confirmed by including DNA from a range of closely related  Candida  species in the  C. albicans  real-time PCR assay. The assay detected fourteen  C. albicans  strains tested but did not detect or cross-react with DNA from any other 19  Candida  species tested. Sensitivity of the assay was tested using various inputs of template DNA from  C. albicans.  The LOD of the assay was found to be between 1-5 cell equivalents ( FIG. 10 ). 
         [0119]    For the  C. glabrata  assay based on the eIF2γ gene, PCR primers GlabF1/GlabR1 were combined with TaqMan probe, GlabA. The specificity of the assay for the detection of  C. glabrata  was confirmed by including DNA from a range of closely related  Candida  species,  Saccharomyces cerevisiae  and  A. fumigatus  in the  C. glabrata  real-time PCR assay. The assay detected ten  C. glabrata  strains tested but did not detect or cross-react with DNA from any other 19  Candida  species tested or with  S. cerevisiae  or  A. fumigatus  DNA. Initial sensitivity of the assay was tested using various inputs of template DNA from  C. glabrata.  The LOD of the assay was found to be ˜2 cell equivalents ( FIG. 11 ). 
         [0120]    For the  C. parapsilosis  assay based on the eIF2γ gene, PCR primers ParaF1/ParaR1 were combined with TaqMan probe, ParA. The specificity of the assay for the detection of  C. parapsilosis  was confirmed by including DNA from a range of closely related  Candida  species,  Saccharomyces cerevisiae  and  A. fumigatus  in the  C. parapsilosis  real-time PCR assay. The assay detected twelve  C. parapsilosis  strains tested but did not detect or cross-react with DNA from any other 19  Candida  species tested or with  S. cerevisiae  or  A. fumigatus  DNA. Initial sensitivity of the assay was tested using various inputs of template DNA from  C. parapsilosis.  The LOD of the assay was found to ˜10 cell equivalents ( FIG. 12 ). 
         [0121]    For the  C. tropicalis  assay based on the eIF2γ gene, PCR primers TropicF1/TropicR1 were combined with TaqMan probe, TropicA. The specificity of the assay for the detection of  C. tropicalis  was confirmed by including DNA from a range of closely related  Candida  species,  Saccharomyces cerevisiae  and  A. fumigatus  in the  C. tropicalis  real-time PCR assay. The assay detected twelve  C. tropicalis  strains tested but did not detect or cross-react with DNA from any other 19  Candida  species tested or with  S. cerevisiae  or  A. fumigatus  DNA. Initial sensitivity of the assay was tested using various inputs of template DNA from  C. tropicalis.  The LOD of the assay was found to ˜20 cell equivalents ( FIG. 13 ). 
         [0122]    For the  C. krusei  assay based on the eIF2γ gene, PCR primers KrusF1/KrusR1 were combined with TaqMan probe, KrusA. The specificity of the assay for the detection of  C. krusei  was confirmed by including DNA from a range of closely related  Candida  species,  Saccharomyces cerevisiae  and  A. fumigatus  in the  C. krusei  real-time PCR assay. The assay detected nine  C. krusei  strains tested but did not detect or cross-react with DNA from any other 19  Candida  species tested or with  S. cerevisiae  or  A. fumigatus  DNA. Initial sensitivity of the assay was tested using various inputs of template DNA from  C. krusei.  The LOD of the assay was found to 2 cell equivalents ( FIG. 14 ). 
         [0123]      Aspergillus  spp 
         [0124]    Primer and Probe Design 
         [0125]    The publicly available sequence information for the eIF2γ gene in  Aspergillus  spp. was aligned with the newly generated sequence information for the eIF2γ gene in  Aspergillus  spp. and analyzed using bioinformatics tools ( FIG. 15-18 ). Primers and probes were designed to amplify and detect  Aspergillus  species. Primers were designed which could amplify more than one species of interest. EF2 — 1_fow was designed to amplify  A. fumigatus  and  A. terreus  .EF2 — 2_fow was designed to amplify  A. flavus  and  A. niger.  EF2 — 1_rev was designed to amplify  A. fumigatus.  EF2 — 3_rev was designed to amplify  A. flavus, A. niger  and  A. terreus.  EF2 — 7_fow was designed to amplify  A. niger  and  A. flavus.  EF2 — 8_fow was designed to amplify  A. terreus.  EF2 — 9 fow was designed to amplify  A. fumigatus.  EF2 — 5 rev was designed to amplify  A. fumigatus, A. flavus,  and  A. terreus.  EF2 — 6_rev was designed to amplify  A. niger  Primers and probes used in these assays are listed in Table 11. 
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 11 
               
             
             
               
                   
               
               
                 Probes and primers designed for real 
               
               
                 time PCR assays for the detection of  
               
               
                 EIF2 target in  Aspergillus  spp. 
               
             
          
           
               
                   
                 Oligo name 
                 Sequence 5&#39;-3&#39; 
               
               
                   
                   
               
               
                   
                 EF2_1_fow 
                 cagccgaagcg 
               
               
                   
                   
               
               
                   
                 EF2_2_fow 
                 cagcccaagcg 
               
               
                   
                   
               
               
                   
                 EF2_3_fow 
                 agccgaagcgYc 
               
               
                   
                   
               
               
                   
                 EF2_4_fow 
                 agcccaagcggcc 
               
               
                   
                   
               
               
                   
                 EF2_5_fow 
                 agccgaagcgtcc 
               
               
                   
                   
               
               
                   
                 EF2_6_fow 
                 agccgaagcgccc 
               
               
                   
                   
               
               
                   
                 EF2_7_fow 
                 agcccaagcggccaga 
               
               
                   
                   
               
               
                   
                 EF2_8_fow 
                 agccgaagcgcccaga 
               
               
                   
                   
               
               
                   
                 EF2_9_fow 
                 agccgaagcgtccagaac 
               
               
                   
                   
               
               
                   
                 EF2_10_fow 
                 agcgccccctgctcc 
               
               
                   
                   
               
               
                   
                 EF2_11_fow 
                 cccgagcagcccgacc 
               
               
                   
                   
               
               
                   
                 EF2_1_rev 
                 cgtgtgcgacgt 
               
               
                   
                   
               
               
                   
                 EF2_3_rev 
                 cgtgagcgagtg 
               
               
                   
                   
               
               
                   
                 EF2_4_rev 
                 cgtgwgcgacgt 
               
               
                   
                   
               
               
                   
                 EF2_5_rev 
                 ggcctggcgcgcaat 
               
               
                   
                   
               
               
                   
                 EF2_6_rev 
                 ggcttggcgcgcaat 
               
               
                   
                   
               
               
                   
                 EF2_7_rev 
                 cgtgtgcgacgtgtccg 
               
               
                   
                   
               
               
                   
                 A.nig_EF2_1 
                 atccggagactctggacct 
               
               
                   
                   
               
               
                   
                 A.terr_EF2_1 
                 cgacgcttacccctctgt 
               
               
                   
                   
               
               
                   
                 A.flav_EF2_1 
                 cagaccccgctaccctt 
               
               
                   
                   
               
               
                   
                 A.fum_EF2_1 
                 acgctcacacctctgtc 
               
               
                   
                   
               
               
                   
                 A.fum_EF2_2 
                 cgacgctcacacctctgtc 
               
               
                   
                   
               
             
          
         
       
     
         [0126]    Real Time PCR 
         [0127]    Assay exclusivity was investigated with the panel outlined in Table 12. 
         [0000]    
       
         
               
             
               
               
             
           
               
                 TABLE 12 
               
               
                   
               
               
                 Panel for assay exclusivity evaluation 
               
               
                 Species name 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                   
                   A. fumigatus  110.46 
               
               
                   
                   A. flavus  117.62 
               
               
                   
                   A. niger  2599 
               
               
                   
                   A. terreus  2729 
               
               
                   
                   A. candidus  102.13 
               
               
                   
                   A. clavatus  1348 
               
               
                   
                   A. glaucus  117314 
               
               
                   
                   A. nidulans  670 
               
               
                   
                   A. versicolor  2916 
               
               
                   
                   N. fischeri  211390 
               
               
                   
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 13 
               
             
             
               
                   
               
               
                 Thermocycling conditions 
               
             
          
           
               
                 Step 
                 Temp 
                 Time 
               
               
                   
               
             
          
           
               
                 UNG 
                 50° C. 
                 2 
                 min 
                   
               
               
                 Denaturation 
                 95° C. 
                 1 
                 min 
               
               
                 Cycling 
                 95° C. 
                 5-10 
                 secs 
                 40 or 45 or 50 cycles 
               
               
                   
                 60° C. 
                 10-30 
                 secs 
               
               
                 Cooling 
                 40° C. 
                 1-2 
                 mins 
               
               
                   
               
             
          
         
       
     
         [0128]    Evaluation of Assay Exclusivity 
         [0129]    The initial evaluation of assay exclusivity was investigated with the panel outlined in Table 12 and the primers and probes outlined in Table 13. The results of these assays performed with annealing times of 95° C. for 10 seconds and 60° C. for 30 seconds for 50 cycles show that the assays were specific for exclusive detection of only the species for which they were designed. 
         [0130]    The  A. flavus, A. niger  and  A. terreus  assays were tested for inclusivity with nine available in-house strains of each species. EF2 — 7_fow and EF2 — 5_rev were used in the  A. flavus  assay. EF2 — 7_fow and EF2 — 6_rev primer pair for the  A. niger  assay and EF2 — 8_fow and EF2 — 5_rev included in the  A. terreus  assay. The annealing conditions of 95° C. for 5 seconds and 60° C. for 10 seconds for 45 cycles were applied to each assay. All strains were detected by the relevant specific probe ( FIG. 19 ). 
         [0131]    The same primer combinations and thermocycling conditions were used in the LOD assays for  A. flavus, A. niger  and  A. terreus.  The LOD for each of the three assays were found to be 5 cell equivalents per reaction. ( FIG. 20 ) 
         [0132]    The same assay conditions were used to test the exclusivity of the  A. flavus, A. niger  and  A. terreus  assays. Each of the three assays was found to be specific, detecting only the species of interest with no cross-reactivity with other closely related  Aspergillus  species included in the assay. All samples were tested in triplicate. ( FIG. 21 ). 
         [0133]    LOD of the  A. fumigatus  assay was performed under thermocycling conditions which included for the  A. fumigatus  annealing conditions of 95° C. for 10 seconds and 60° C. for 30 seconds for 50 cycles. The LOD for this assay was found to be 10-1 cell equivalents ( FIG. 22 ). 
         [0134]    Discussion 
         [0135]    The number of yeast and fungal infections among immunocomprised patients is escalating. Contributing to this increase is the growing resistance of many yeast and fungal species to antifungal drugs. There is therefore a need to develop a fast, accurate diagnostic method to enable early diagnosis of yeast and fungal species. Early diagnosis will enable the selection of a specific narrow spectrum antibiotic or antifungal to treat the infection. The current invention provides for sequences and/or diagnostic assays to detect and identify one or more yeast and fungal species. The current inventors have exploited the sequence of the eIF2 γ gene in  Candida  and  Aspergillus  species to design primers and probes specific for regions of this gene. The eIF2 γ sequence has significant intragenic sequence heterogeneity in some regions, while having significant homogeneity in others, a trait, which makes eIF2 γ an ideal candidate for the design of primers and probes directed towards the detection of yeast and fungal species specific targets and for the detection of genus specific diagnostic targets respectively. 
         [0136]    The current invention allows the detection of yeast and fungal species but also allows distinction between  Candida  and  Aspergillus  species. It is a further object of the invention to allow the discrimination between different  Candida  species and different  Aspergillus  species. The words “comprises/comprising” and the words “having/including” when used herein with reference to the present invention are used to specify the presence of stated features, integers, steps or components but does not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof. 
         [0137]    It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable sub-combination. 
         [0138]    In so far as any sequence disclosed herein differs from its counterpart in the attached sequence listing in PatentIn3.3 software, the sequences within this body of text are to be considered as the correct version. 
         [0139]    SEQ IDs 
         [0140]    Sites of probes, oligonucleotides etc. are shown in bold and underlined. 
         [0141]    N or x=any nucleotide; w=a/t, m=a/c, r=a/g, k=g/t, s=c/g, y=c/t, h=a/t/c, v=a/g/c, d=a/g/t, b=g/t/c. In some cases, specific degeneracy options are indicated in parenthesis: e.g.: (a/g) is either A or G. 
         [0000]    
       
         
               
               
               
             
               
               
             
           
               
                 SEQ ID NO. 1: P1-CaneIF2: 
                 CGATAATGCTCCGATCGTGCCTA 
                   
               
               
                   
               
               
                 SEQ ID NO. 2: P1-AspeIF2: 
                 CGCTCACACCTCTGTCGCCCGAA 
               
               
                   
               
               
                 SEQ ID NO. 3: AspeIF2-F: 
                 CTTAAGTCTGCGATGAAGA 
               
               
                   
               
               
                 SEQ ID NO. 4: AspeIF2-R: 
                 GTAATGTTACGCTCCAACTC 
               
               
                   
               
               
                 SEQ ID NO. 5: CaneIF2-F: 
                 GCTGCCATTGAAATTATGAA 
               
               
                   
               
               
                 SEQ ID NO. 6: CaneIF2-R: 
                 GAACCACCTGCAACACC 
               
               
                   
               
             
          
           
               
                 SEQ 1D NO.7: 
                   
               
               
                 &gt;CA2700E4 eIF2 γ\(CaneIF2-F) eIF2 γ sequence generated for  C . 
               
               
                 
                   albicans 
                 
               
               
                 TGCATAATAAAGTTGATTTGATGAGAGAAGAATCAGCCTTGGAACACGAAAAATCT 
               
               
                   
               
               
                 ATCATTCAGTTTATTAGAGGTACAATTGCCGATAATGCTCCGATCGTGCCTATTTCT 
               
               
                   
               
               
                 GCTCAATTGAAATACAACATTGATGCAGTGAATCAATTTATTGTTAACTACATACCT 
               
               
                   
               
               
                 GTGCCAATGAGAGACTTTACTGCTTCACCAAGATTGATCGTTATCAGATCTTTCGAT 
               
               
                   
               
               
                 GTGAACAAGCCTGGTGCAGATGTAGACGAATTGAAAGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ 1D NO. 8: 
               
               
                 &gt;A765\(EF) sequence generated for  C .  albicans   
               
               
                 ATTATTTTGCATAATAAAGTTGATTTGATGAGAGAAGAATCAGCYTTGGAACACGAAAAA 
               
               
                   
               
               
                 TCTATCATTCAGTTTATTAGAGGTACAATTGCCGATAATGCTCCGATCGTGCCTATTTCT 
               
               
                   
               
               
                 GCTCAATTRAAATACAACATTGATGCAGTGAATCAATTTATYGTTAACTACATACCTGTG 
               
               
                   
               
               
                 CCAATGAGAGACTTTACTGCTTCACCAAGATTGATYGTTATCAGATCTTTCGATGTGAAC 
               
               
                   
               
               
                 AAGCCTGGTGCAGATGTAGACGAATTGAAAGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ 1D NO. 9: 
               
               
                 &gt;A3156\(EF) sequence generated for  C .  albicans   
               
               
                 ATTATTTTGCATAATAAAGTTGATTTGATGAGAGAAGAATCAGCYTTGGRACACGAAAAA 
               
               
                   
               
               
                 TCTATCATTCAGTTTATTAGAGGTACAATTGCCGATAATGCTCCGATCGTGCCTATT 
               
               
                   
               
               
                 TCTGCTCAATTGAAATACAACATTGATGCAGTGAATCAATTTATYGTTAACTACATACCT 
               
               
                   
               
               
                 GTGCCAATGAGAGACTTTACTGCTTCACCAAGATTGATYGTTATCAGATCTTTCGATG 
               
               
                   
               
               
                 TGAACAAGCCTGGTGCAGATGTAGACGAATTGAAAGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ 1D NO. 10: 
               
               
                 &gt;A562\(EF) sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCATAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CCTTGGAACACGAAAAATCTATCATTCAGTTTATTAGAGGTACAATTGCCGATAAT 
               
               
                   
               
               
                 GCTCCGATCGTGCCTATTTCTGCTCAATTGAAATACAACATTGATGCAGTGAATCAA 
               
               
                   
               
               
                 TTTATTGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTG 
               
               
                   
               
               
                 ATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAA 
               
               
                   
               
               
                 AGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 11: 
               
               
                 &gt;CG3897E5\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C . 
               
               
                 
                   glabrata 
                 
               
               
                 GTTAAAGCACGTTATTATTCTACAGAACAAGGTCGATTTAATGCGTGAAGAAAGCG 
               
               
                   
               
               
                 CACTAGAACATGAAAAGTCTATCCTGAAATTTATCAGAGGTACTATTGCTGACGGT 
               
               
                   
               
               
                 GCTCCAATTGTCCCAATTTCCGCTCAATTGAAATACAACATCGATGCAGTTAATGAA 
               
               
                   
               
               
                 TTTATCGTGAAGACTATCCCTGTTCCACCAAGAGATTTCATGCTTTCTCCACGTTTG 
               
               
                   
               
               
                 ATTGTCATTCGTTCTTTCGATGTTAACAAGCCAGGTGCTGAAATCGATGATTTGAAG 
               
               
                   
               
               
                 GGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 12: 
               
               
                 &gt;CG8018E6\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C . 
               
               
                 
                   glabrata 
                 
               
               
                 GTTAAAGCACGTTATTATTCTACAGAACAAGGTCGATTTAATGCGTGAAGAAAGCG 
               
               
                   
               
               
                 CACTAGAACATGAAAAGTCTATCCTGAAATTTATCAGAGGTACTATTGCTGACGGT 
               
               
                   
               
               
                 GCTCCAATTGTCCCAATTTCCGCTCAATTGAAATACAACATCGATGCAGTTAACGAA 
               
               
                   
               
               
                 TTTATCGTGAAGACTATCCCTGTTCCACCAAGAGATTTCATGCTTTCTCCACGTTTG 
               
               
                   
               
               
                 ATTGTCATTCGTTCTTTCGATGTTAACAAGCCAGGTGCTGAAATCGATGATTTGAAG 
               
               
                   
               
               
                 GGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 13: 
               
               
                 &gt;CG4692E7\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C . 
               
               
                 
                   glabrata 
                 
               
               
                 GTTAAAGCACGTTATTATTCTACAGAACAAGGTCGATTTAATGCGTGAAGAAAGCG 
               
               
                   
               
               
                 CACTAGAACATGAAAAGTCTATCCTGAAATTTATCAGAGGTACTATTGCTGACGGT 
               
               
                   
               
               
                 GCTCCAATTGTCCCAATTTCCGCTCAATTGAAATACAACATCGATGCAGTCAATGAA 
               
               
                   
               
               
                 TTTATCGTGAAGACTATCCCTGTTCCACCAAGAGATTTCATGCTTTCTCCACGTTTG 
               
               
                   
               
               
                 ATTGTCATTCGTTCTTTCGATGTTAACAAGCCAGGTGCTGAAATCGATGATTTGAAG 
               
               
                   
               
               
                 GGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 14: 
               
               
                 &gt;G138\(EF) sequence generated for  C .  glabrata   
               
               
                 GTTAAAGCACGTTATTATTCTACAGAACAAGGTCGATTTAATGCGTGAAGAAAGCG 
               
               
                   
               
               
                 CACTAGAACATGAAAAGTCTATCCTGAAATTTATCAGAGGTACTATTGCTGACGGT 
               
               
                   
               
               
                 GCTCCAATTGTCCCAATTTCCGCTCAATTGAAATACAACATCGATGCAGTCAATGAA 
               
               
                   
               
               
                 TTTATCGTGAAGACTATCCCTGTTCCACCAAGAGATTTCATGCTTTCTCCACGTTTG 
               
               
                   
               
               
                 ATTGTCATTCGTTCTTTCGATGTTAACAAGCCAGGTGCTGAAATCGATGATTTGAAG 
               
               
                   
               
               
                 GGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 15: 
               
               
                 &gt;CK3165E9\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C . 
               
               
                 
                   krusei 
                 
               
               
                 TGKTGTGATTKTACAAAATAAAGTTGATTTGATGAAGAAAGAAGCAGCTTTAGAGC 
               
               
                   
               
               
                 ACGAAAAATCTATTTTGAAGTTTATCAAGGGTACTATTGCTGATGGTGCTCCTATTA 
               
               
                   
               
               
                 TCCCAATTTCTGCTCAGTTGAAATACAACATTGATGCAGTTAACATGTGTATGGTCA 
               
               
                   
               
               
                 AGTCTATTCCTGTTCCAATTAGAGACTTTACTGCAGTTCCAAGATTAATGGTTATTA 
               
               
                   
               
               
                 GATCTTTCGATGTTAATAAGCCTGGTGCAGAAATTGCAGATTTGAAAGGTGGTGTT 
               
               
                   
               
               
                 GCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 16: 
               
               
                 &gt;K9560\(EF) sequence generated for  C .  krusei   
               
               
                 TGTTGTGATTTTACAAAATAAAGTTGATTTGATGAAGAAAGAAGCAGCTTTAGAGCACGAAAA 
               
               
                   
               
               
                 ATCTATTTTGAAGTTTATCAAGGGTACTATTGCTGATGGTGCTCCTATTATCCCAATTTCTG 
               
               
                   
               
               
                 CTCAGTTGAAATAYAACATTGATGCAGTTAACATGTGTATGGTCAAGTCTATTCCTGTT 
               
               
                   
               
               
                 CCAATTAGAGACTTTACYGCAGTTCCAAGATTAATGGTTATTAGATCTTTCGATGTTAATAA 
               
               
                   
               
               
                 GCCTGGTGCAGAAATTGCAGATTTGAAAGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 17: 
               
               
                 &gt;K6055\(EF) sequence generated for  C .  krusei   
               
               
                 TGTTGTGATTTTACAAAATAAAGTTGATTTGATGAAGAAAGAAGCAGCTTTAGAGC 
               
               
                   
               
               
                 ACGAAAAATCTATTTTGAAGTTTATCAAGGGTACTATTGCTGATGGTGCTCCTATTA 
               
               
                   
               
               
                 TCCCAATTTCTGCTCAGTTGAAATATAACATTGATGCAGTTAACATGTGTATGGTCA 
               
               
                   
               
               
                 AGTCTATTCCTGTTCCAATTAGAGACTTTACCGCAGTTCCAAGATTAATGGTTATTA 
               
               
                   
               
               
                 GATCTTTCGATGTTAATAAGCCTGGTGCAGAAATTGCAGATTTGAAAGGTGGTGTT 
               
               
                   
               
               
                 GCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 18: 
               
               
                 &gt;K573E\(EF) sequence generated for  C .  krusei   
               
               
                 TGKTGTGATTKTACAAAATAAAGTTGATTTGATGAAGAAAGAAGCAGCTTTAGAGC 
               
               
                   
               
               
                 ACGAAAAATCTATTTTGAAGTTTATCAAGGGTACTATTGCTGATGGTGCTCCTATTA 
               
               
                   
               
               
                 TCCCAATTTCTGCTCAGTTGAAATATAACATTGATGCAGTTAACATGTGTATGGTCA 
               
               
                   
               
               
                 AGTCTATTCCTGTTCCAATTAGAGACTTTACCGCAGTTCCAAGATTAATGGTTATTA 
               
               
                   
               
               
                 GATCTTTCGATGTTAATAAGCCTGGTGCAGAAATTGCAGATTTGAAAGGTGGTGTT 
               
               
                   
               
               
                 GCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 19: 
               
               
                 &gt;CP96141E14\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  parapsilosis   
               
               
                 GTTGAAGCATGTTATAATTTTGCAAAACAAGGTTGATTTGATGAGAGAAGARTCRG 
               
               
                   
               
               
                 CATTGGAACATGAAAAGTCTATTATTCAGTTCATAAGAGGTACCATAGCTGATGGT 
               
               
                   
               
               
                 GCACCAATAGTTCCAATTTCGGCACAATTGAAATATAATATCGATGCCGTCAATCA 
               
               
                   
               
               
                 ATTCATAGTCAACTCCATACCTGTCCCAGTTAGAGAYTTTACTGCATCACCAAGATT 
               
               
                   
               
               
                 GATTGTTATTAGGTCTTTYGATGTSAACAAACCTGGTGCTGATGTTGATGACTTGAA 
               
               
                   
               
               
                 GGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 20: 
               
               
                 &gt;CP2194E16\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  parapsilosis   
               
               
                 GTTGAAGCACGTTATTATTTTGCAAAACAAAGTTGATTTAATGAGAAAGGAGTCAG 
               
               
                   
               
               
                 CTTTGGAACATGAAAAGTCCATCATTCAGTTCATCAGAGGTACTATAGCTGATGGT 
               
               
                   
               
               
                 GCCCCAATTGTTCCAATTTCAGCACAATTGAAGTATAATATCGACGCCGTCAATCAA 
               
               
                   
               
               
                 TTCATCGTAAACTCAATACCAGTTCCAGTCAGGGACTTTACTGCATCCCCTAGGTTA 
               
               
                   
               
               
                 ATTGTTATTAGGTCTTTTGATGTGAACAAACCTGGTGCTGACGTTGATGATTTGAAA 
               
               
                   
               
               
                 GGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 21: 
               
               
                 &gt;P96143\(EF) sequence generated for  C .  parapsilosis   
               
               
                 TGTTATAATTTTACAAAATAAGGTTGATTTGATGAGAGAAGAGTCKGCATTGGAGC 
               
               
                   
               
               
                 ATGARAAGTCGATACTTCAATTCATAAGAGGTACTATAGCMGATGGTGCTCCAATT 
               
               
                   
               
               
                 GTTCCAATTTCAGCTCAATTGAAATACAATATCGACGCCGTCAATCAATTTATAGTA 
               
               
                   
               
               
                 AATTCCATACCSGTTCCAATTAGGGATTTCAATGCCTCACCAAGGTTGATTGTTATT 
               
               
                   
               
               
                 CGATCATTTGATGTGAAYAAACCTGGTGCTGATGTCGAYGATTTGAAGGGAGGTGT 
               
               
                   
               
               
                 TGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 22: 
               
               
                 &gt;P-604\(EF)) sequence generated for  C .  parapsilosis   
               
               
                 GTTGAAGCACGTTATTATTTTGCAAAACAAAGTTGATTTAATGAGAAAGGAGTCAG 
               
               
                   
               
               
                 CTTTGGAACATGAAAAGTCCATCATTCAGTTCATCAGAGGTACTATAGCTGATGGT 
               
               
                   
               
               
                 GCCCCAATTGTTCCAATTTCAGCACAATTGAAGTATAATATCGACGCCGTCAATCAA 
               
               
                   
               
               
                 TTCATCGTAAACTCAATACCAGTTCCAGTCAGGGACTTTACTGCATCCCCTAGGTTA 
               
               
                   
               
               
                 ATTGTTATTAGGTCTTTTGATGTGAACAAACCTGGTGCTGACGTTGATGATTTGAAA 
               
               
                   
               
               
                 GGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 23: 
               
               
                 &gt;CT2311E19\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  tropicalis   
               
               
                 GGTCGATTTGATGAGAGAAGAATCTGCCTTGGAACATGAGAAATCCATTCTTCAAT 
               
               
                   
               
               
                 TCATCAGAGGTACTATTGCAGACAATGCTCCTATTGTCCCAATTTCTGCCCAATTGA 
               
               
                   
               
               
                 AATACAACATCGATGCCGTTAACCAATTTATTGTCAATTATATCCCAGTTCCATTGA 
               
               
                   
               
               
                 GAGACTTTTCCGCTTCCCCAAGATTGATTGTCATCAGATCTTTTGATGTCAACAAGC 
               
               
                   
               
               
                 CAGGTTCCGATGTCGAAGACTTGAAAGGGGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 24: 
               
               
                 &gt;T2424\(EF) sequence generated for  C .  tropicalis   
               
               
                 GGTCGATTTGATGAGAGAAGAATCTGCCTTGGAACATGAGAAATCCATTCTTCAAT 
               
               
                   
               
               
                 TCATCAGAGGTACTATTGCAGACAATGCTCCTATTGTCCCAATTTCTGCCCAATTGA 
               
               
                   
               
               
                 AATACAACATCGATGCCGTTAACCAATTTATTGTCAATTATATCCCAGTTCCATTGA 
               
               
                   
               
               
                 GAGACTTTTCCGCTTCCCCAAGATTGATTGTCATCAGATCTTTTGATGTCAACAAGC 
               
               
                   
               
               
                 CAGGTTCCGATGTCGAAGACTTGAAAGGGGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 25: 
               
               
                 &gt;T94\(EF) sequence generated for  C .  tropicalis   
               
               
                 GTCATKATTTTGCAGAACAAGGTCGATTTGATGAGAGAAGAATCTGCCTTGGAACATGAGAA 
               
               
                   
               
               
                 ATCCATTCTTCAATTCATCAGAGGTACTATTGCAGACAATGCTCCTATTGTCCCAATTTCT 
               
               
                   
               
               
                 GCCCAATTGAAATACAACATCGATGCCGTTAACCAATTTATTGTCAATTATATCCCAGTTC 
               
               
                   
               
               
                 CATTGAGAGACTTTTCCGCTTCCCCAAGATTGATTGTCATCAGATCTTTTGATGTCAA 
               
               
                   
               
               
                 CAAGCCAGGTTCCGATGTCGAAGACTTGAAAGGGGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 26: 
               
               
                 &gt;T-3895\(EF) sequence generated for  C .  tropicalis   
               
               
                 GGTCGATTTGATGAGAGAAGAATCTGCCTTGGAACATGAGAAATCCATTCTTCAAT 
               
               
                   
               
               
                 TCATCAGAGGTACTATTGCAGACAATGCTCCTATTGTCCCAATTTCTGCCCAATTGA 
               
               
                   
               
               
                 AATACAACATCGATGCCGTTAACCAATTTATTGTCAATTATATCCCAGTTCCATTGA 
               
               
                   
               
               
                 GAGACTTTTCCGCTTCCCCAAGATTGATTGTCATCAGATCTTTTGATGTCAACAAGC 
               
               
                   
               
               
                 CAGGTTCCGATGTCGAAGACTTGAAAGGGGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 27: 
               
               
                 &gt;CD3949E21\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  dubliniensis   
               
               
                 AATAAAGTTGATTTGATGAGAGAAGAATCAGCTTTGGAACATGAAAAATCCATTAT 
               
               
                   
               
               
                 TCAGTTCATCAGAGGCACAATTGCTGATAACGCCCCAATTGTGCCTATTTCTGCGCA 
               
               
                   
               
               
                 ATTGAAATACAACATTGATGCTGTAAATCAATTTATTGTGAACTACATACCTGTGCC 
               
               
                   
               
               
                 AATGAGAGACTTTACTGCTTCACCAAGATTGATCGTTATTAGATCTTTTGATGTGAA 
               
               
                   
               
               
                 CAAGCCTGGTGCGGATGTTGACGAATTGAAAGGGGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 28: 
               
               
                 &gt;CD7987E22\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  dubliniensis   
               
               
                 TGAAACATGTCATTATTTTGCAGAATAAAGTTGATTTGATGAGAGAAGAATCAGCT 
               
               
                   
               
               
                 TTGGAACATGAAAAATCCATTATTCAGTTCATCAGAGGCACAATTGCTGATAACGC 
               
               
                   
               
               
                 CCCAATTGTGCCTATTTCTGCGCAATTGAAATACAACATTGATGCTGTAAATCAATT 
               
               
                   
               
               
                 TATTGTGAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTGAT 
               
               
                   
               
               
                 CGTTATTAGATCTTTTGATGTGAACAAGCCTGGTGCGGATGTTGACGAATTGAAAG 
               
               
                   
               
               
                 GGGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 29: 
               
               
                 &gt;CD8501E23\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  dubliniensis   
               
               
                 ATTGAAACATGTCATTATTTTGCAGAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CTTTGGAACATGAAAAATCCATTATTCAGTTCATCAGAGGCACAATTGCTGATAAC 
               
               
                   
               
               
                 GCCCCAATTGTGCCTATTTCTGCGCAATTGAAATACAACATTGATGCTGTAAATCAA 
               
               
                   
               
               
                 TTTATTGTGAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTG 
               
               
                   
               
               
                 ATCGTTATTAGATCTTTTGATGTGAACAAGCCTGGTGCGGATGTTGACGAATTGAAA 
               
               
                   
               
               
                 GGGGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 30: 
               
               
                 &gt;CGU2672E25\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  guilliermondii   
               
               
                 TTGCAAAATAAGGTGGATCTTATGAGAGAAGAATCGGCGTTGGAGCACCAAAAATC 
               
               
                   
               
               
                 GATTTTGAATTTTATTAAAGGAACCATCGCTGACGGTGCCCCCATCGTCCCTATCTC 
               
               
                   
               
               
                 GGCCCAATTGAAGTACAACATCGATGCCGTGAACCAATTCATAGTCAACTCGATCC 
               
               
                   
               
               
                 CCGTTCCTCCTCGTGACTTTTCCGCATCTCCTCGGTTGATCGTGATTCGTTCTTTCGA 
               
               
                   
               
               
                 CGTCAATAAACCCGGTTCTGAAATTGATGACTTGAAGGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 31: 
               
               
                 &gt;CGU6021E26\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  guillermondii   
               
               
                 GTTGAAACATGTTATCATCTTGCAAAATAAGGTGGATCTTATGAGAGAAGAATCGG 
               
               
                   
               
               
                 CGTTGGAGCACCAAAAATCGATTTTGAATTTTATTAAAGGAACCATCGCTGATGGT 
               
               
                   
               
               
                 GCACCTATCGTCCCTATCTCGGCCCAGTTGAAGTACAACATCGATGCCGTGAACCA 
               
               
                   
               
               
                 ATTCATAGTCAACTCGATCCCCGTTCCTCCTCGTGACTTTTCCGCATCTCCTCGGTTG 
               
               
                   
               
               
                 ATCGTGATTCGTTCTTTCGACGTCAATAAACCCGGTTCTGAGATCGATGACTTGAAA 
               
               
                   
               
               
                 GGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 32: 
               
               
                 &gt;Guil8167\(EF) sequence generated for  C .  guillermondii   
               
               
                 GTTGAAACATGTTATCATCTTGCAAAATAAGGTGGATCTTATGAGAGAAGAATCGG 
               
               
                   
               
               
                 CGTTGGAGCACCAAAAATCGATTTTGAATTTTATTAAAGGAACCATCGCTGATGGT 
               
               
                   
               
               
                 GCACCTATCGTCCCTATCTCGGCCCAGTTGAAGTACAACATCGATGCCGTGAACCA 
               
               
                   
               
               
                 ATTCATAGTCAACTCGATCCCCGTTCCTCCTCGTGACTTTTCCGCATCTCCTCGGTTG 
               
               
                   
               
               
                 ATCGTGATTCGTTCTTTCGACGTCAATAAACCCGGTTCTGAGATCGATGACTTGAAA 
               
               
                   
               
               
                 GGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 33: 
               
               
                 &gt;CN1922E33\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  norvegiensis   
               
               
                 GTTGAAACACGTTGTTATTTTACAAAATAAAGTTGATTTGATGAAAAAGGAAGCTG 
               
               
                   
               
               
                 CGTTGGAACACGAAAAATCTATTCTTAAGTTCATCAAGGGTACGATCGCTGATGGA 
               
               
                   
               
               
                 GCTCCAATCATTCCTATTTCTGCACAATTGAAATATAACATTGATGCTGTTAACATG 
               
               
                   
               
               
                 TGTATGGTAAACTCCATTCCAATTCCAATGAGAGATTTTACTGCTCAGCCAAGATTA 
               
               
                   
               
               
                 ATGGTCATCAGATCTTTCGATGTTAACAAACCTGGTGCAGAAATAAATGATTTGAA 
               
               
                   
               
               
                 AGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 34: 
               
               
                 &gt;FAM1E45\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C .  famata   
               
               
                 ATTGARTCATGTTATTATCTTACAAAACAAGGTTGATTTAATGARAGAGGAATCAGCTTTGGAAC 
               
               
                   
               
               
                 ATCAGAAATCTATTTTGAGTTTCATCAGAGGTACTATTGCAGATGGTGCTCCAATTGTTCCAA 
               
               
                   
               
               
                 TTTCTGCCCAATTAAAATATAATATCGATGCTGTTAATCAATTTATTGTGAACTCTATTCCAA 
               
               
                   
               
               
                 TTCCTCCAAGAGACTTCATGGCTACTCCAAGATTGATCGTTATTAGATCTTTCGATGTTAATAAA 
               
               
                   
               
               
                 CCAGGTGCCGAGATTGATGACTTGAAGGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 35: 
               
               
                 &gt;FAM2E46\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C .  famata   
               
               
                 GTGATTATCTTACAAAATAAGGTTGATTTAATGAGAGAAGAGTCAGCTTTAGAGCATCAAAA 
               
               
                   
               
               
                 GTCCATTTTGAGTTTCATCAGAGGTACTATTGCTGATGGTGCTCCAATTGTTCCAATTTCTGC 
               
               
                   
               
               
                 TCAATTAAAATATAATATTGATGCTGTCAATCAATTTATTGTTAATTCTATTCCAATTCCGCC 
               
               
                   
               
               
                 AAGAGACTTSATGGCTACTCCAAGATTGATCGATATTAGATCATTCGATGTTAATAAACCAGG 
               
               
                   
               
               
                 GGCAGAAATTGATGACTTGAAGGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 36: 
               
               
                 &gt;FAM5E47\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C .  famata   
               
               
                 ATTGAAGCATGTGATCATCTTACAAAATAAGGTTGATTTAATGAGAGAAGAATCTG 
               
               
                   
               
               
                 CTTTAGAGCATCAAAAGTCCATTTTGAGTTTCATCAGAGGTACTATTGCTGATGGTG 
               
               
                   
               
               
                 CTCCAATTGTTCCAATTTCTGCTCAATTAAAATATAATATTGATGCTGTCAATCAAT 
               
               
                   
               
               
                 TTATTGTTAATTCTATTCCAATTCCGCCAAGAGACTTGATGGCTACTCCAAGATTGA 
               
               
                   
               
               
                 TCGATATTAGATCATTCGATGTTAATAAACCAGGTGCTGAAATTGATGACTTGAAG 
               
               
                   
               
               
                 GGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 37: 
               
               
                 &gt;CH53E48\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  haemuloni   
               
               
                 CGTTATCATTTTGCAGAACAAGGTGGATTTGATGAGAGAAGAGTCTGCTTTGGAGC 
               
               
                   
               
               
                 ACCAGAAATCGATCTTGAGTTTTATCAGAGGTACCATTGCCGATGGCGCTCCTATCG 
               
               
                   
               
               
                 TGCCAATTTCCGCCCAATTGAAGTACAACATTGACGCTGTCAACCAGTTGATCTGCG 
               
               
                   
               
               
                 ACTACATCCCTGTTCCTCCTAGAGACTTCATGGCCTCGCCACGTTTGATCGTCATTA 
               
               
                   
               
               
                 GGTCTTTCGATGTCAACAAGCCAGGTGCCGAGATCGAGGACTTGAAGGGAGGTGTT 
               
               
                   
               
               
                 GCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 38: 
               
               
                 &gt;CH54E49\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  haemuloni   
               
               
                 CGTTATCATTTTGCAGAACAAGGTGGATTTGATGAGAGAAGAGTCTGCTTTGGAGC 
               
               
                   
               
               
                 ACCAGAAATCGATCTTGAGTTTTATCAGAGGTACCATTGCCGATGGCGCTCCTATCG 
               
               
                   
               
               
                 TGCCAATTTCCGCCCAATTGAAGTACAACATTGACGCTGTCAACCAGTTGATCTGCG 
               
               
                   
               
               
                 ACTACATCCCTGTTCCTCCTAGAGACTTCATGGCCTCGCCACGTTTGATCGTCATTA 
               
               
                   
               
               
                 GGTCTTTCGATGTCAACAAGCCAGGTGCCGAGATCGAGGACTTGAAGGGAGGTGTT 
               
               
                   
               
               
                 GCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 39: 
               
               
                 &gt;CH55E50\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  haemuloni   
               
               
                 CGTTATCATTTTGCAGAACAAGGTGGATTTGATGAGAGAAGAGTCTGCTTTGGAGC 
               
               
                   
               
               
                 ACCAGAAATCGATCTTGAGTTTTATCAGAGGTACCATTGCCGATGGCGCTCCTATCG 
               
               
                   
               
               
                 TGCCAATTTCCGCCCAATTGAAGTACAACATTGACGCTGTCAACCAGTTGATCTGCG 
               
               
                   
               
               
                 ACTACATCCCTGTTCCTCCTAGAGACTTCATGGCCTCGCCACGTTTGATCGTCATTA 
               
               
                   
               
               
                 GGTCTTTCGATGTCAACAAGCCAGGTGCCGAGATCGAGGACTTGAAGGGAGGTGTT 
               
               
                   
               
               
                 GCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 40: 
               
               
                 &gt;CKF57E51\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C .  kefyr   
               
               
                 TTATTCTTCAGAACAAGGTGGATCTAATGAGAGAAGACTCCGCTCTAGAGCATCAA 
               
               
                   
               
               
                 AAATCTATTTTGAAGTTTATCAGAGGTACTATTGCCGATGGTGCACCAATTGTTCCA 
               
               
                   
               
               
                 ATTTCTGCTCAATTGAAGTACAATATTGATGCCGTTAACGAATTTATCGTTAAGAGT 
               
               
                   
               
               
                 ATCCCAGTTCCACAAAGAGACTTCTTAGCATCTCCAAGATTGATCGTCATCCGTTCT 
               
               
                   
               
               
                 TTTGACGTCAACAAGCCAGGTGCAGAAATTGATGATTTGAAGGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 41: 
               
               
                 &gt;CKF60E52\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C .  kefyr   
               
               
                 TTATTCTTCAGAACAAGGTGGATCTAATGAGAGAAGACTCCGCTCTAGAGCATCAA 
               
               
                   
               
               
                 AAATCTATTTTGAAGTTTATCAGAGGTACTATTGCCGATGGTGCACCAATTGTTCCA 
               
               
                   
               
               
                 ATTTCTGCTCAATTGAAGTACAATATTGATGCCGTTAACGAATTTATCGTTAAGAGT 
               
               
                   
               
               
                 ATCCCAGTTCCACAAAGAGACTTCTTAGCATCTCCAAGATTGATCGTCATCCGTTCT 
               
               
                   
               
               
                 TTTGACGTCAACAAGCCAGGTGCAGAAATTGATGATTTGAAGGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 42: 
               
               
                 &gt;CKF3898E53\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  C . 
               
               
                 
                   kefyr 
                 
               
               
                 TTATTCTTCAGAACAAGGTGGATCTAATGAGAGAAGACTCCGCTCTAGAGCATCAA 
               
               
                   
               
               
                 AAATCTATTTTGAAGTTTATCAGAGGTACTATTGCCGATGGTGCACCAATTGTTCCA 
               
               
                   
               
               
                 ATTTCTGCTCAATTGAAGTACAATATTGATGCCGTTAACGAATTTATCGTTAAGAGT 
               
               
                   
               
               
                 ATCCCAGTTCCACAAAGAGACTTCTTAGCATCTCCAAGATTGATCGTCATCCGTTCTTTT 
               
               
                   
               
               
                 GACGTCAACAAGCCAGGTGCAGAAATTGATGATTTGAAGGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 43: 
               
               
                 &gt;Lus8013\(EF) sequence generated for  C .  lusitaniae   
               
               
                 ACAAGGTCGACTTGATGAGAGAGGAGCTGGCGTTGGAGCACCAGAAATCCATTTTG 
               
               
                   
               
               
                 AGTTTCATCCGAGGCACCATCGCCGATGGCGCCCCTATCGTGCCTATCTCGGCCCAA 
               
               
                   
               
               
                 TTGAAGTACAACATTGACGCTGTCAACCAGATCATCTGCGACTACATTCCCGTGCCT 
               
               
                   
               
               
                 CCTCGAGACTTTATGGCGTCGCCCCGTTTGATTGTCATTAGATCTTTCGATGTGAAC 
               
               
                   
               
               
                 AAGCCAGGTGCCGAGATTGACGACTTGAAAGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 44: 
               
               
                 &gt;S.ciff-2305\(EF) sequence generated for  S .  cifferii   
               
               
                 ATTGCAACATGTTATCATTCTGCAAAACAAGGTGGATCTCATGAAGGAGGAGTCCG 
               
               
                   
               
               
                 CCCTCGAGCATCACAAGAGCATTCTCAAGTTCATTCGAGGCACCATTGCCGACGGA 
               
               
                   
               
               
                 TCCCCAGTTATCCCCATTTCTGCCCAATTGAAGTACAACATTGACGCTGTCAACGAG 
               
               
                   
               
               
                 TTCATTGTGTCCAAGATCCCCATCCCCGTCAGAGATTTCCAGGCAACCCCACGATTG 
               
               
                   
               
               
                 ATTGTCATTAGATCATTCGATATCAACCGGCCCGGTTCCGAAATCGACGAGCTCCGT 
               
               
                   
               
               
                 GGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 45: 
               
               
                 &gt;S.ciff-5295\(EF) sequence generated for  S .  cifferii   
               
               
                 TCATGCAAAATAAGGTGGATCTCATGAAGGAGGAGTCCGCCCTCGAGCATCACAAG 
               
               
                   
               
               
                 AGCATTCTCAAGTTCATTCGGGGCACAATTGCCGACGGATCCCCAGTTATCCCCATT 
               
               
                   
               
               
                 TCTGCACAGCTGAAGTACAACATTGATGCTGTCAATGAGTTCATTGTTTCCAAGATC 
               
               
                   
               
               
                 CCAATCCCAGTCAGAGATTTCCAGGCCACCCCACGATTGATTGTCATTAGATCCTTC 
               
               
                   
               
               
                 GATATTAACCGTCCAGGTTCCGAAATCGACGAGCTTCGTGGCGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 46: 
               
               
                 &gt;CU50E58\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  utilis   
               
               
                 ACAGGTGGATTTGATGCGTCCAGACTCTGCCTTGGAGCATGAGAAGTCGATTTTGA 
               
               
                   
               
               
                 AGTTCATCAGAGGTACCATTGCCGATGGTGCTCCAATCGTCCCAATCTCTGCTCAAT 
               
               
                   
               
               
                 TGAAATACAACATTGATGCTGTCAACGAATTCATCGTCAAGACTATCCCAGTGCCA 
               
               
                   
               
               
                 CCAAGAGACTTCAGTGCCTCTCCAAGACTCATTGTGATCCGTTCTTTTGACGTTAAC 
               
               
                   
               
               
                 AAACCAGGTGCCGAAATTGACGACTTGAAAGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 47: 
               
               
                 &gt;CV94E62\(EF)\(CaneIF2-F) eIF2 γ sequence generated for 
               
               
                   C .  viswanathii   
               
               
                 GGTCGATTTGATGAGGGAAGAGTCCGCCTTGGAGCACGAGAAATCCATTCTCCAAT 
               
               
                   
               
               
                 TCATCAGAGGTACCATTGCCGACAATGCTCCTATTGTGCCAATTTCGGCGCAGTTGA 
               
               
                   
               
               
                 AGTACAACATCGACGCCGTGAACCAGTTTATTGTCAACTACATCCCAGTGCCATTG 
               
               
                   
               
               
                 AGAGACTTTTCTGCATCTCCAAGATTGATAGTCATCAGATCCTTTGATGTCAACAAG 
               
               
                   
               
               
                 CCAGGTTCGGACGTCGAAGAGTTGAAAGGTGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO. 48: 
               
               
                 &gt;F419-64\(AspEF-F) sequence generated for  A .  fumigatus   
               
               
                 CGAAGCGTCCAGAACTCCCCGAGCAGCCCGACCCAGAGACTCTCGATTTGTCGACG 
               
               
                   
               
               
                 CTCACACCTCTGTCGCCCGAAATTATTGCGCGCCAGGCCACAATCAACATCGGTACT 
               
               
                   
               
               
                 ATCGGACACGTCGCACACGGCAAGTCGACTGTTGTGAAGGCTATCTCGGAGGTGCA 
               
               
                   
               
               
                 GACTGTCCGGTTCAAAAATGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 49: 
               
               
                 &gt;F483-61\(AspEF-F) sequence generated for  A .  fumigatus   
               
               
                 AGCGCCCCCTGCTCCTCAGCCGAAGCGTCCAGAACTCCCCGAGCAGCCCGACCCAG 
               
               
                   
               
               
                 AGACTCTCGATTTGTCGACGCTCACACCTCTGTCGCCCGAAATTATTGCGCGCCAGG 
               
               
                   
               
               
                 CCACAATCAACATCGGTACTATCGGACACGTCGCACACGGCAAGTCGACTGTTGTG 
               
               
                   
               
               
                 AAGGCTATCTCGGAGGTGCAGACTGTCCGGTTCAAAAATGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 50: 
               
               
                 &gt;F7273\(AspEF-F) sequence generated for  A .  fumigatus   
               
               
                 AGCGCCCCCTGCTCCTCAGCCGAAGCGTCCAGAACTCCCCGAGCAGCCCGACCCAG 
               
               
                   
               
               
                 AGACTCTCGATTTGTCGACGCTCACACCTCTGTCGCCCGAAATTATTGCGCGCCAGG 
               
               
                   
               
               
                 CCACAATCAACATCGGTACTATCGGACACGTCGCACACGGCAAGTCGACTGTTGTG 
               
               
                   
               
               
                 AAGGCTATCTCGGAGGTGCAGACTGTCCGGTTCAAAAATGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 51: 
               
               
                 &gt;F6951\(AspEF-F) sequence generated for  A .  fumigatus   
               
               
                 AGCGCCCCCTGCTCCTCAGCCGAAGCGTCCAGAACTCCCCGAGCAGCCCGACCCAG 
               
               
                   
               
               
                 AGACTCTCGATTTGTCGACGCTCACACCTCTGTCGCCCGAAATTATTGCGCGCCAGG 
               
               
                   
               
               
                 CCACAATCAACATCGGTACTATCGGACACGTCGCACACGGCAAGTCGACTGTTGTG 
               
               
                   
               
               
                 AAGGCTATCTCGGAGGTGCAGACTGTCCGGTTCAAAAATGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 52: 
               
               
                 &gt;F133-61\(AspEF-F) eIF2 γ sequence generated for  A .  fumigatus   
               
               
                 CCGAAGCGTCCAGAACTCCCCGAGCAGCCCGACCCAGAGACTCTCGATTTGTCGAC 
               
               
                   
               
               
                 GCTCACACCTCTGTCGCCCGAAATTATTGCGCGCCAGGCCACAATCAACATCGGTA 
               
               
                   
               
               
                 CTATCGGACACGTCGCACACGGCAAGTCGACTGTTGTGAAGGCTATCTCGGAGGTG 
               
               
                   
               
               
                 CAGACTGTCCGGTTCAAAAATGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ ID NO. 53: 
               
               
                 &gt;1085-eIF2 γ\(AspeIF2-F) eIF2 γ sequence generated for  N .  fischeri   
               
               
                 CGGCCCCCGTTCCTCAGCCGAAGCGTCCAGAACTGCCCGAGCAGCCCAACCCAGAG 
               
               
                   
               
               
                 ACTCTCGATCTGTCGACACTGACGCCTCTGTCTCCCGAGATTATCGCGCGCCAGGCC 
               
               
                   
               
               
                 ACAATCAACATCGGTACTATCGGACACGTCGCTCACGGCAAGTCGACCGTGGTGAA 
               
               
                   
               
               
                 GGCTATTTCGGAGGTGCAGACTGTCCGGTTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ ID NO. 54: 
               
               
                 &gt;5138eIF2 γ\(AspeIF2-F) eIF2 γ sequence generated for  A .  clavatus   
               
               
                 AGGGCTCCATGCCCCCAGTTCCTCAACCGAAGCGCCCAGAACTGCCCGAGCAGCCTGACCCAGA 
               
               
                   
               
               
                 GACCATCGATCTGTCGAAACTGACGCCTCTGTCCCCCGAAATTATCGCGCGCCAGGCCACGATCA 
               
               
                   
               
               
                 ACATTGGTACCATCGGACACGTCGCTCACGGCAAGTCGACCGTGGTGAAGGCTATCTCGGAGGT 
               
               
                   
               
               
                 GCAGACGGTCCGTTTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 55: 
               
               
                 &gt;C1348\(AspEF-F) sequence generated for  A .  clavatus   
               
               
                 CATGCCCCCAGTTCCTCAACCGAAGCGCCCAGAACTGCCCGAGCAGCCTGACCCAG 
               
               
                   
               
               
                 AGACCATCGATCTGTCGAAACTGACGCCTCTGTCCCCCGAAATTATCGCGCGCCAG 
               
               
                   
               
               
                 GCCACGATCAACATTGGTACCATCGGACACGTCGCTCACGGCAAGTCGACCGTGGT 
               
               
                   
               
               
                 GAAGGCTATCTCGGAGGTGCAGACGGTCCGTTTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 56: 
               
               
                 &gt;C2391\(AspEF-F) sequence generated for  A .  clavatus   
               
               
                 AAGCGCCCAGAACTCCCCGAGCAGCCTGACCCAGAGACCATCGATCTGTCGAAACT 
               
               
                   
               
               
                 GACGCCTTTGTCCCCCGAAATCATCGCGCGCCAGGCCACGATCAACATTGGTACCA 
               
               
                   
               
               
                 TCGGGCACGTCGCTCACGGCAAGTCGACCGTGGTGAAGGCTATTTCGGAGGTGCAA 
               
               
                   
               
               
                 ACTGTTCGTTTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 57: 
               
               
                 &gt;NIG2864\(AspEF-F) sequence generated for  A .  niger   
               
               
                 TTCCTCCTCCCCAGCCCAAGCGGCCAGAGCTGCCCGAGCAGCCGAATCCGGAGACT 
               
               
                   
               
               
                 CTGGACCTGTCCACCCTGACTCCTTTGACCCCCGAAATTATTGCGCGCCAAGCCACA 
               
               
                   
               
               
                 ATCAACATTGGCACCATCGGTCACGTCGCTCACGGCAAGTCGACGGTCGTTAAGGC 
               
               
                   
               
               
                 TATCTCCGAGGTCCAGACTGTCCGTTTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 58: 
               
               
                 &gt;NIG6727\(AspEF-F) sequence generated for  A .  niger   
               
               
                 TTCCTCCTCCCCAGCCCAAGCGGCCAGAGCTGCCCGAGCAGCCGAATCCGGAGACT 
               
               
                   
               
               
                 CTGGACCTGTCCACCCTGACTCCTTTGACCCCCGAAATTATTGCGCGCCAAGCCACA 
               
               
                   
               
               
                 ATCAACATTGGCACCATCGGTCACGTCGCTCACGGCAAGTCGACGGTCGTTAAGGC 
               
               
                   
               
               
                 TATCTCCGAGGTCCAGACTGTCCGTTTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 59: 
               
               
                 &gt;NIG328399\(AspEF-F) sequence generated for  A .  niger   
               
               
                 TTCCTCCTCCCCAGCCCAAGCGGCCAGAGCTGCCCGAGCAGCCGAATCCGGAGACT 
               
               
                   
               
               
                 CTGGACCTGTCCACCCTGACTCCTTTGACCCCCGAAATTATTGCGCGCCAAGCCACA 
               
               
                   
               
               
                 ATCAACATTGGCACCATCGGTCACGTCGCTCACGGCAAGTCGACGGTCGTTAAGGC 
               
               
                   
               
               
                 TATCTCCGAGGTCCAGACTGTCCGTTTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 60: 
               
               
                 &gt;NIGMA5184\(AspEF-F) sequence generated for  A .  niger   
               
               
                 TTCCTCCTCCCCAGCCCAAGCGGCCAGAGCTGCCCGAGCAGCCGAATCCGGAGACT 
               
               
                   
               
               
                 CTGGACCTGTCCACGCTGACTCCTTTGACCCCCGAAATTATTGCGCGCCAAGCCACA 
               
               
                   
               
               
                 ATCAACATTGGCACCATCGGTCACGTCGCTCACGGCAAGTCGACGGTCGTTAAGGC 
               
               
                   
               
               
                 TATCTCCGAGGTCCAGACTGTCCGTTTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ ID NO. 61 
               
               
                 &gt;118.46 eIF2 γ\(AspeIF2-F) eIF2 γ sequence generated  
               
               
                 for  A .  terreus   
               
               
                 AGGACTCTGCTCTTCCCCCCCAGCCGAAGCGCCCAGAGCTTCCTGAACAACCCAACCCAG 
               
               
                   
               
               
                 ACACCCTCGATCTGTCGACGCTTACCCCTCTGTCGCCCGAAATCATTGCGCGCCAGGC 
               
               
                   
               
               
                 CACCATCAACATCGGTACCATTGGTCACGTCGCTCACGGAAAGTCGACGGTCGTCAA 
               
               
                   
               
               
                 GGCCATCTCAGAGGTCCAGACCGTTCGATTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 62: 
               
               
                 &gt;T2729\(AspEF-F) sequence generated for  A .  terreus   
               
               
                 AGCCGAAGCGCCCAGAGCTTCCTGAACAACCCAACCCAGACACCCTCGATCTGTCG 
               
               
                   
               
               
                 ACGCTTACCCCTCTGTCGCCCGAAATTATTGCGCGCCAGGCCACCATCAACATCGGT 
               
               
                   
               
               
                 ACCATTGGTCACGTCGCTCACGGAAAGTCGACGGTTGTCAAGGCCATCTCAGAGGT 
               
               
                   
               
               
                 CCAGACCGTTCGATTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 63: 
               
               
                 &gt;T601-65\(AspEF-F) sequence generated for  A .  terreus   
               
               
                 AGCCGAAGCGCCCAGAGCTTCCTGAACAACCCAACCCAGACACCCTCGATCTGTCG 
               
               
                   
               
               
                 ACGCTTACCCCTCTGTCGCCCGAAATTATTGCGCGCCAGGCCACCATCAACATCGGT 
               
               
                   
               
               
                 ACCATTGGTCACGTCGCTCACGGAAAGTCGACGGTTGTCAAGGCCATCTCAGAGGT 
               
               
                   
               
               
                 CCAGACCGTTCGATTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 64: 
               
               
                 &gt;T5677\(AspEF-F) sequence generated for  A .  terreus   
               
               
                 AGCCGAAGCGCCCAGAGCTTCCTGAACAACCCAACCCAGACACCCTCGATCTGTCG 
               
               
                   
               
               
                 ACGCTTACCCCTCTGTCGCCCGAAATTATTGCGCGCCAGGCCACCATCAACATCGGT 
               
               
                   
               
               
                 ACCATTGGTCACGTCGCTCACGGAAAGTCGACGGTTGTCAAGGCCATCTCAGAGGT 
               
               
                   
               
               
                 CCAGACCGTTCGATTCAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 65: 
               
               
                 &gt;FL625-66\(AspEF-F) sequence generated for  A .  flavus   
               
               
                 CCCCCTGTTTCTCAGCCCAAGCGGCCAGAGTTGCCCGAACAGCCAGACCCCGCTAC 
               
               
                   
               
               
                 CCTTGACCTGTCGACCCTGACCCCTCTGTCGCCCGAAATCATTGCGCGCCAGGCCAC 
               
               
                   
               
               
                 TATTAACATTGGTACCATCGGACACGTCGCTCACGGAAAGTCAACAGTGGTCAAGG 
               
               
                   
               
               
                 CTATCTCAGAGGTTCAGACTGTCCGTTTCAAAAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 66: 
               
               
                 &gt;FL2008\(AspEF-F) sequence generated for  A .  flavus   
               
               
                 CCCCCTGTTTCTCAGCCCAAGCGGCCAGAGTTGCCCGAACAGCCAGACCCCGCTAC 
               
               
                   
               
               
                 CCTTGACCTGTCGACCCTGACCCCTCTGTCGCCCGAAATCATTGCGCGCCAGGCCAC 
               
               
                   
               
               
                 TATTAACATTGGTACCATCGGACACGTCGCTCACGGAAAGTCAACAGTGGTCAAGG 
               
               
                   
               
               
                 CTATCTCAGAGGTTCAGACTGTCCGTTTCAAAAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 67: 
               
               
                 &gt;FL2199\(AspEF-F) sequence generated for  A .  flavus   
               
               
                 CCCCCTGTTTCTCAGCCCAAGCGGCCAGAGTTGCCCGAACAGCCAGACCCCGCTAC 
               
               
                   
               
               
                 CCTTGACCTGTCGACCCTGACCCCTCTGTCGCCCGAAATCATTGCGCGCCAGGCCAC 
               
               
                   
               
               
                 TATTAACATTGGTACCATCGGACACGTCGCTCACGGAAAGTCAACAGTGGTCAAGG 
               
               
                   
               
               
                 CTATCTCAGAGGTTCAGACTGTCCGTTTCAAAAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 68: 
               
               
                 &gt;V2916\(AspEF-F) sequence generated for  A .  versicolor   
               
               
                 TCAGCCGAAACGACCAGAGCTACCGGAGCAGCCCAACCCAGACACCCTCGACCTG 
               
               
                   
               
               
                 ACCACATTAACTCCCCTGTCCCCGGAAATTATTGCCCGCCAGGCCACGATCAACATC 
               
               
                   
               
               
                 GGCACCATTGGTCACGTCGCTCACGGAAAGTCAACGGTGGTGAAGGCTATCTCAGA 
               
               
                   
               
               
                 AGTCCAGACTGTCAGATTTAAGAATGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 69: 
               
               
                 &gt;V1323\(AspEF-F) sequence generated for  A .  versicolor   
               
               
                 TCAGCCGAAACGACCAGAGCTACCGGAGCAGCCCAACCCAGACACCCTCGACCTG 
               
               
                   
               
               
                 ACCACATTAACTCCCCTGTCCCCGGAAATTATTGCCCGCCAGGCCACGATCAACATC 
               
               
                   
               
               
                 GGCACCATTGGTCACGTCGCTCACGGAAAGTCAACGGTGGTGAAGGCTATCTCAGA 
               
               
                   
               
               
                 AGTCCAGACTGTCAGATTTAAGAATGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 70: 
               
               
                 &gt;N100-2\(AspEF-F) sequence generated for  A .  nidulans   
               
               
                 ACGACCGGAACTACCGGAGCAACCCAACCCAGAAACGCTCGACCTGTCTACACTAA 
               
               
                   
               
               
                 CTCCTCTGTCACCTGAGATTATCGCCCGCCAGGCTACGATTAACATCGGTACCATTG 
               
               
                   
               
               
                 GCCACGTCGCTCACGGTAAGTCAACGGTGGTGAAGGCTATTTCAGAGGTTCAAACT 
               
               
                   
               
               
                 GTCCGATTTAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 71: 
               
               
                 &gt;N589-65\(AspEF-F) sequence generated for  A .  nidulans   
               
               
                 ACGACCGGAACTACCGGAGCAACCCAACCCAGAAACGCTCGACCTGTCTACACTAA 
               
               
                   
               
               
                 CTCCTCTGTCACCTGAGATTATCGCCCGCCAGGCTACGATTAACATCGGTACCATTG 
               
               
                   
               
               
                 GCCACGTCGCTCACGGTAAGTCAACGGTGGTGAAGGCTATTTCAGAGGTTCAAACT 
               
               
                   
               
               
                 GTCCGATTTAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 72: 
               
               
                 &gt;N6365\(AspEF-F) sequence generated for  A .  nidulans   
               
               
                 TTCATACCTGTTTCTCAGCCGAAACGACCGGAACTACCGGAGCAACCCAACCCAGA 
               
               
                   
               
               
                 AACGCTCGACCTGTCTACACTAACTCCTCTGTCACCTGAGATTATCGCCCGCCAGGC 
               
               
                   
               
               
                 TACGATTAACATCGGTACCATTGGCCACGTCGCTCACGGTAAGTCAACGGTGGTGA 
               
               
                   
               
               
                 AGGCTATTTCAGAGGTTCAAACTGTCCGATTTAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ 1D NO. 73: 
               
               
                 &gt;N670-78\(AspEF-F) sequence generated for  A .  nidulans   
               
               
                 TTCATACCTGTTTCTCAGCCGAAACGACCGGAACTACCGGAGCAACCCAACCCAGA 
               
               
                   
               
               
                 AACGCTTGACCTGTCTACACTAACTCCTTTGTCACCTGAGATTATCGCCCGCCAGGC 
               
               
                   
               
               
                 TACGATTAACATCGGTACCATTGGTCACGTCGCTCACGGTAAGTCAACGGTGGTGA 
               
               
                   
               
               
                 AGGCTATTTCAGAGGTTCAAACTGTCCGATTTAAGAACGAGTTGGAGCGTAACATTAC 
               
               
                   
               
               
                 SEQ ID No 74: 
               
               
                 &gt;gi|68470315|ref|XM_715569.1|  Candida   albicans  SC5314  
               
               
                 putative translation initiation factor eIF2 gamma subunit  
               
               
                 (CaO19_11699), mRNA 
               
               
                 ATGTCATACGACGATATAGAAAATGCCACTCCTGATATTGTTATTGGGAGTACTATA 
               
               
                   
               
               
                 GAGGAACCTGAAGAAGATTACCAAGTGGAAAGTGACAATGAGTTACAAGCCGCAG 
               
               
                   
               
               
                 ACCATGAGTCATCGCAAATAAATGAAGAATCAGCCAAAGGCAAAAAGTCAGTTGC 
               
               
                   
               
               
                 ATTTACTGGATTGGATGAAGACGAGGAAAATGCAGAGGAATTGGCCAGAAAGGAG 
               
               
                   
               
               
                 TTTGAAGAAGGTGGTGGATTGCCTGAACAACCAGAAAACCCAGATTTCAATGAGTT 
               
               
                   
               
               
                 AACACCTTTATCTCCCGAGATTATCAACAGGCAAGCCACCATTAATATTGGTACCAT 
               
               
                   
               
               
                 TGGTCATGTCGCCCACGGGAAGTCTACTGTTGTCAGGGCTATCTCTGGTGTCCAGAC 
               
               
                   
               
               
                 CGTTCGTTTCAAGGATGAATTAGAAAGAAACATTACTATCAAGTTAGGTTACGCCA 
               
               
                   
               
               
                 ATGCCAAAATTTACAAATGTGATAACCCAGAGTGTCCAGAACCAGATTGTTACAGA 
               
               
                   
               
               
                 TCATTCAAATCAGATAAGGAAATAAGACCAAAATGTCAAAGAGCTGGCTGTGACG 
               
               
                   
               
               
                 GTCGCTACAAATTGTTAAGACATGTCTCTTTTGTTGATTGTCCAGGACATGATATTT 
               
               
                   
               
               
                 TGATGAGTACTATGTTGTCAGGTGCTGCCGTGATGGATGCCGCCTTGTTGTTGATTG 
               
               
                   
               
               
                 CCGGTAATGAAAGTTGTCCACAACCCCAGACTTCTGAGCATTTGGCTGCCATTGAA 
               
               
                   
               
               
                 ATTATGAAATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGA 
               
               
                   
               
               
                 AGAATCAGCCTTGGAACACGAAAAATCTATCATTCAGTTTATTAGAGGTACAATTG 
               
               
                   
               
               
                 CCGATAATGCTCCGATCGTGCCTATTTCTGCTCAATTGAAATACAACATTGATGCAG 
               
               
                   
               
               
                 TGAATCAATTTATTGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCAC 
               
               
                   
               
               
                 CAAGATTGATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGAC 
               
               
                   
               
               
                 GAATTGAAAGGAGGTGTTGCAGGTGGTTCTATTTTGACTGGTGTTTTTAAGATTGGT 
               
               
                   
               
               
                 GATGAGATCGAGATTAGACCTGGTATCGTCACCAAAGATGATCAAGGAAAGATTCA 
               
               
                   
               
               
                 ATGTAAACCTATATTCTCGAACGTGGTTTCCTTGTTTGCTGAGCATAACGATTTGAA 
               
               
                   
               
               
                 ATTTGCTGTTCCTGGTGGTTTGATTGGTGTTGGTACTAAAGTTGATCCTACGTTGTGT 
               
               
                   
               
               
                 AGGGCTGATAGATTGGTTGGTCAAGTTGTTGGTGCAAAAGGAAACTTGCCCTCTAT 
               
               
                   
               
               
                 TTACGCTGATATTGAGATAAACTATTTCCTATTAAGAAGATTGTTGGGTGTCAAAAC 
               
               
                   
               
               
                 TGAAGGTCAAAAGCAAGGTGCTAAAGTTCGTAAGTTGGAACAATCTGAAGTGTTGA 
               
               
                   
               
               
                 TGGTAAATATTGGTTCTACTGCAACTGGTGCTAGAGTGGTTGCTGTTAAAGCAGATA 
               
               
                   
               
               
                 TGGCTCGTTTACAATTGACTACACCAGCCTGTACAGAAATCAACGAAAAAATTGCG 
               
               
                   
               
               
                 TTGTCTAGACGTATTGAAAAGCATTGGCGTTTGATTGGTTGGGCCACTATCAAGAA 
               
               
                   
               
               
                 AGGTACAGCATTAGAACCAATTTCTTAA 
               
               
                   
               
               
                 SEQ ID NO 75: 
               
               
                 &gt;gi|68470576|ref|XM_715441.1|  Candida   albicans  SC5314  
               
               
                 translation initiation factor eIF2 gamma subunit  
               
               
                 CaO19_4223) partial mRNA 
               
               
                 ATGTCATACGACGATATAGAAAATGCCACTCCTGATATTGTTATTGGGAGTACTATA 
               
               
                   
               
               
                 GAGGAACCTGAAGAAGATTACCAAGTGGAAAGTGACAATGAGTTACAAGCCGCAG 
               
               
                   
               
               
                 ACCATGAGTCATCGCAAATAAATGAAGAATCAGCCAAAGGCAAAAAGTCAGTTGC 
               
               
                   
               
               
                 ATTTACTGGATTGGATGAAGACGAGGAAAATGCAGAGGAATTGGCCAGAAAGGAG 
               
               
                   
               
               
                 TTTGAAGAAGGTGGTGGATTGCCTGAACAACCAGAAAACCCAGATTTCAATGAGTT 
               
               
                   
               
               
                 AACACCTTTATCTCCCGAGATTATCAACAGGCAAGCCACCATTAATATTGGTACCAT 
               
               
                   
               
               
                 TGGTCATGTCGCCCACGGGAAGTCTACTGTTGTCAGGGCTATCTCTGGTGTCCAGAC 
               
               
                   
               
               
                 CGTTCGTTTCAAGGATGAATTAGAAAGAAACATTACTATCAAGTTAGGTTACGCCA 
               
               
                   
               
               
                 ATGCCAAAATTTACAAATGTGATAACCCAGAGTGTCCAGAACCAGATTGTTACAGA 
               
               
                   
               
               
                 TCATTCAAATCAGATAAGGAAATAAGACCAAAATGTCAAAGAGCTGGCTGTGACG 
               
               
                   
               
               
                 GTCGCTACAAATTGTTAAGACATGTCTCTTTTGTTGATTGTCCAGGACATGATATTT 
               
               
                   
               
               
                 TGATGAGTACTATGTTGTCAGGTGCTGCCGTGATGGATGCCGCCTTGTTGTTGATTG 
               
               
                   
               
               
                 CCGGTAATGAAAGTTGTCCACAACCCCAGACTTCTGAGCATTTGGCTGCCATTGAA 
               
               
                   
               
               
                 ATTATGAAATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGA 
               
               
                   
               
               
                 AGAATCAGCCTTGGAACACGAAAAATCTATCATTCAGTTTATTAGAGGTACAATTG 
               
               
                   
               
               
                 CCGATAATGCTCCGATCGTGCCTATTTCTGCTCAATTGAAATACAACATTGATGCAG 
               
               
                   
               
               
                 TGAATCAATTTATTGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCAC 
               
               
                   
               
               
                 CAAGATTGATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGAC 
               
               
                   
               
               
                 GAATTGAAAGGAGGTGTTGCAGGTGGTTCTATTTTGACTGGTGTTTTTAAGATTGGT 
               
               
                   
               
               
                 GATGAGATCGAGATTAGACCTGGTATCGTCACCAAAGATGATCAAGGAAAGATTCA 
               
               
                   
               
               
                 ATGTAAACCTATATTCTCGAACGTGGTTTCCTTGTTTGCTGAGCATAACGATTTGAA 
               
               
                   
               
               
                 ATTTGCTGTTCCTGGTGGTTTGATTGGTGTTGGTACTAAAGTTGATCCTACGTTGTGT 
               
               
                   
               
               
                 AGGGCTGATAGATTGGTTGGTCAAGTTGTTGGTGCAAAAGGAAACTTGCCCTCTAT 
               
               
                   
               
               
                 TTACGCTGATATTGAGATAAACTATTTCCTATTAAGAAGATTGTTGGGTGTCAAAAC 
               
               
                   
               
               
                 TGAAGGTCAAAAGCAAGGTGCTAAAGTTCGTAAGTTGGAACAATCTGAAGTGTTGA 
               
               
                   
               
               
                 TGGTAAATATTGGTTCTACTGCAACTGGTGCTAGAGTGGTTGCTGTTAAAGCAGATA 
               
               
                   
               
               
                 TGGCTCGTTTACAATTGACTACACCAGCCTGTACAGAAATCAACGAAAAAATTGCG 
               
               
                   
               
               
                 TTGTCTAGACGTATTGAAAAGCATTGGCGTTTGATTGGTTGGGCCACTATCAAGAA 
               
               
                   
               
               
                 AGGTACAGCATTAGAACCAATTTCTTAA 
               
               
                   
               
               
                 SEQ ID NO 76: 
               
               
                 &gt;gi|50290356|re|XM_447610.1|  Candida   glabrata  CBS138  
               
               
                 hypothetical protein(CAGL0I08327g) partial mRNA 
               
               
                 ATGTCTGATTTGCAAGATCAAGAGCCAACTATTATTATCAATGGTGATCTTCCACCA 
               
               
                   
               
               
                 GTAGAAGAAGAGGAAGTCTATGAGCAGGAAGAGCAAGAGGAAGTTGTTGAGGAGA 
               
               
                   
               
               
                 AGCCAAAGAAGAAAGTTGCCTTTACCGGTCTAGAGGATGGTGAATCTGAGGAAGA 
               
               
                   
               
               
                 GAAGAGAAAGAGAGAGTTTGAAGAAGGTGGTGGATTGCCAGAGCAGCCAGAAAAC 
               
               
                   
               
               
                 CCAGACTTTACTAAGTTGAACCCACTTTCTGCTGAGATTATTAACAGACAAGCTACT 
               
               
                   
               
               
                 ATCAACATCGGTACTATTGGTCATGTCGCTCACGGTAAGTCTACTGTTGTCAGAGCC 
               
               
                   
               
               
                 ATCTCTGGTGTCCAAACCGTTCGTTTCAAGGATGAGTTGGAACGTAACATTACTATC 
               
               
                   
               
               
                 AAGCTGGGTTATGCCAATGCTAAGATATATAAGTGTCAAGAGCCTACATGTCCAGA 
               
               
                   
               
               
                 ACCAGACTGTTACAGATCTTTCAAGTCTGACAAAGAAATTAATCCAAAGTGTCAAA 
               
               
                   
               
               
                 GACCAGGTTGCCCAGGCCGTTACAAACTTGTTCGTCACGTCTCTTTCGTCGATTGTC 
               
               
                   
               
               
                 CAGGTCACGATATTCTAATGAGTACTATGTTGTCCGGTGCCGCTGTCATGGACGCAG 
               
               
                   
               
               
                 CCTTGTTATTGATCGCCGGTAATGAATCTTGTCCACAACCTCAAACTTCTGAACATT 
               
               
                   
               
               
                 TGGCTGCCATTGAAATCATGAAGTTAAAGCACGTTATTATTCTACAGAACAAGGTC 
               
               
                   
               
               
                 GATTTAATGCGTGAAGAAAGCGCACTAGAACATGAAAAGTCTATCCTGAAATTTAT 
               
               
                   
               
               
                 CAGAGGTACTATTGCTGACGGTGCTCCAATTGTCCCAATTTCCGCTCAATTGAAATA 
               
               
                   
               
               
                 CAACATCGATGCAGTCAATGAATTTATCGTGAAGACTATCCCTGTTCCACCAAGAG 
               
               
                   
               
               
                 ATTTCATGCTTTCTCCACGTTTGATTGTCATTCGTTCTTTCGATGTTAACAAGCCAGG 
               
               
                   
               
               
                 TGCTGAAATCGATGATTTGAAGGGTGGTGTTGCAGGTGGTTCCATCTTGAACGGTGT 
               
               
                   
               
               
                 GTTCAAGTTGGGTGATGAGATTGAAATTAGACCAGGTATTGTCACTAAGGATGATA 
               
               
                   
               
               
                 AGGGTAAGATCCAATGTAAGCCAATTTTCTCCAACATTGTCTCTCTATTTGCTGAAC 
               
               
                   
               
               
                 AAAATGACTTGAAGTTTGCAGTCCCAGGTGGTCTGATTGGTGTTGGTACAAAGGTC 
               
               
                   
               
               
                 GATCCTACCTTATGTAGAGCTGATCGTCTTGTCGGTCAAGTTGTCGGTGCCAAGGGT 
               
               
                   
               
               
                 CACCTACCAAGCATTTACACAGATATTGAAATCAACTACTTCCTACTGCGTCGTCTA 
               
               
                   
               
               
                 TTAGGTGTTAAGACTGAGAAACAAGCCAAGGTCAGAAAGCTGGTTGCCAACGAAG 
               
               
                   
               
               
                 TTCTTATGGTTAACATTGGTTCTACTGCCACTGGTGCCCGTGTCGTTGCTGTCAAGG 
               
               
                   
               
               
                 CTGATATGGCTAGATTGCAACTAACATCCCCAGCATGTACAGAAATCAATGAAAAG 
               
               
                   
               
               
                 ATTGCTCTCTCTAGACGTATTGACAAGCACTGGCGTTTAATTGGTTGGGCTACAATC 
               
               
                   
               
               
                 AAGAAAGGTACCACTTTGGAACCAGTTGTCTAA 
               
               
                   
               
               
                 SEQ ID NO. 77: 
               
               
                 &gt;XM_746974.2_ Published eIF2 γ sequence for  A .  fumigatus:   
               
               
                   Aspergillus   fumigatus  Af293 translation initiation 
               
               
                 factor EF-2 gamma subunit (AFUA_4G07580), partial mRNA 
               
               
                 ATGGCTACCAACGGCGATTTTACCGACGATGAATCGCAGCCTGGCTCTCCCATGTTG 
               
               
                   
               
               
                 GATGCGGCGAACGGCCAGGATGATATTGAAGAACAGGAACGTCTTGACGTGGAAG 
               
               
                   
               
               
                 AGAAGCCCCTTAAGTCTGCGATGAAGAAAGGTGCAGCGCCCCCTGCTCCTCAGCCG 
               
               
                   
               
               
                 AAGCGTCCAGAACTCCCCGAGCAGCCCGACCCAGAGACTCTCGATTTGTCGACGCT 
               
               
                   
               
               
                 CACACCTCTGTCGCCCGAAATTATTGCGCGCCAGGCCACAATCAACATCGGTACTA 
               
               
                   
               
               
                 TCGGACACGTCGCACACGGCAAGTCGACTGTTGTGAAGGCTATCTCGGAGGTGCAG 
               
               
                   
               
               
                 ACTGTCCGGTTCAAAAATGAGTTGGAGCGTAACATTACCATCAAGCTTGGTTATGC 
               
               
                   
               
               
                 CAACGCGAAGATCTACAAGTGCGACAACCCTGGGTGCCCGCGCCCGACGTGCTTCA 
               
               
                   
               
               
                 AGAGTTACAAGAGTGAGAAGGAGATCGACCCTCCATGTGAGAGAGAAGGATGCAC 
               
               
                   
               
               
                 AGGTCGTTACAGATTGTTGAGACATGTCTCGTTCGTTGACTGCCCTGGGCACGATAT 
               
               
                   
               
               
                 TCTCATGAGTACCATGTTGTCAGGTGCCGCCGTCATGGACGCCGCCCTTTTGCTGAT 
               
               
                   
               
               
                 TGCCGGAAACGAAGCTTGCCCCCAGCCTCAGACTTCGGAGCACTTAGCAGCTATTG 
               
               
                   
               
               
                 AAATCATGAAGCTCAGCCATATCATCATTCTGCAGAACAAGGTTGATCTGATGAGG 
               
               
                   
               
               
                 GAAGACGGTGCTCTGCAACATTACCAATCAATCCTGAAGTTCATTCGTGGTACTGTT 
               
               
                   
               
               
                 GCCGATGGCTCTCCTATCATTCCCATCTCTGCTCAGCTCAAGTACAACATCGACGCT 
               
               
                   
               
               
                 GTCAACGAATACCTTGTCTCGCACATCCCAGTTCCCGTCCGTGACTTCACTGCTTCG 
               
               
                   
               
               
                 CCTCACATGATTGTCATTCGTTCCTTCGACGTCAACAAACCCGGTGCGGAGATCGAT 
               
               
                   
               
               
                 GAGTTGAAGGGTGGTGTTGCAGGTGGCTCTATCCTCACTGGTGTGCTGAAGCTGAA 
               
               
                   
               
               
                 CGACGAGATTGAAATTCGCCCCGGTCTCGTTACCAAGGATGAGAACGGAAAGATTC 
               
               
                   
               
               
                 AGTGCCGCCCCATCTTCTCCCGTGTCGTCTCGCTCTTCGCTGAGCACAACGATCTGA 
               
               
                   
               
               
                 AGTTCGCTGTCCCTGGTGGTCTAATCGGTGTCGGAACCCGTGTCGACCCTACCCTGT 
               
               
                   
               
               
                 GCCGTGCCGATCGTCTTGTTGGTTTCGTCCTGGGTCACCGTGGCCGTTTGCCAGCCA 
               
               
                   
               
               
                 TCTACACTGAACTGGAGGTCAACTACTTCCTCCTGCGTCGTCTGCTCGGTGTCAAGA 
               
               
                   
               
               
                 CCGCCGACGGCAAGCAGGCCAAGGTCGCCAAGCTCACCAAGAACGAAGTCCTCAT 
               
               
                   
               
               
                 GGTTAACATCGGCTCTACGGCTACTGGTGCTAAGGTTATGGGTGTGAAGGCTGATG 
               
               
                   
               
               
                 CTGCCAAGCTCAGCTTGACCAGCCCGGCTTGTACAGAGATTGGAGAGAAGATTGCT 
               
               
                   
               
               
                 ATCAGCCGGAGAATTGACAAGCATTGGCGTCTGATCGGCTGGGCCAACATTGTCGC 
               
               
                   
               
               
                 TGGCAACACTCTTGAGCCCATTCTGAACTAG 
               
               
                   
               
               
                 SEQ ID NO. 78: 
               
               
                 &gt;XM_001267265.1_ Published eIF2 sequence for  N .  fischeri : 
               
               
                   Neosartorya   fischeri  NRRL 181 translation initiation factor  
               
               
                 EF-2 gamma subunit, putative (NFIA_108620) partial mRNA 
               
               
                 ATGGCTACCAACGGCGATTTTACCGACGATGAATCGCAGCCTGGCTCTCCCATGCT 
               
               
                   
               
               
                 GGATGCGGCGAACGGCCAGGATGACATTGAAGAACAGGAGCCTCTTGACGTGGAA 
               
               
                   
               
               
                 GAGAAGCCCCTCAAGTCTGCAATGAAGAAAGGTTCAGCGCCCCCTGCTCCTCAGCC 
               
               
                   
               
               
                 GAAGCGTCCAGAACTCCCCGAGCAGCCCGACCCAGAGACTCTCGAATTGTCGACAC 
               
               
                   
               
               
                 TCACGCCTCTGTCGCCCGAGATTATTGCGCGCCAGGCCACAATCAACATCGGTACT 
               
               
                   
               
               
                 ATCGGACACGTCGCTCACGGCAAGTCGACTGTGGTGAAGGCTATTTCGGAGGTGCA 
               
               
                   
               
               
                 GACTGTCCGGTTCAAAAATGAGTTGGAGCGTAATATTACCATCAAGCTTGGTTATG 
               
               
                   
               
               
                 CCAACGCGAAGATCTACAAGTGCGACAACCCTGAGTGCCCGCGCCCGACGTGCTTC 
               
               
                   
               
               
                 AAGAGTTACAAGAGTGAGAAGGAGATCGACCCTCCATGTGAGAGAGAAGGATGCA 
               
               
                   
               
               
                 CAGGTCGTTACAGATTGTTGAGACATGTCTCGTTCGTTGACTGCCCTGGGCACGATA 
               
               
                   
               
               
                 TTCTCATGAGTACCATGTTGTCAGGTGCCGCCGTCATGGACGCCGCCCTTTTGCTGA 
               
               
                   
               
               
                 TTGCCGGAAACGAAGCTTGCCCCCAGCCTCAGACTTCGGAGCACTTGGCAGCTATT 
               
               
                   
               
               
                 GAAATCATGAAGCTCAGCCACATCATCATTCTGCAGAACAAGGTTGATCTGATGAG 
               
               
                   
               
               
                 GGAAGACGGTGCTCTTCAACATTACCAATCAATCCTGAAGTTCATTCGTGGTACTGT 
               
               
                   
               
               
                 TGCCGATGGTTCTCCTATCATTCCCATCTCTGCTCAGCTCAAGTACAACATCGACGC 
               
               
                   
               
               
                 TGTCAACGAATACCTTGTCTCGCACATCCCAGTTCCCGTCCGTGACTTCACTGCTTC 
               
               
                   
               
               
                 GCCTCACATGATTGTCATCCGTTCCTTCGACGTCAACAAGCCCGGTGCGGAGATCG 
               
               
                   
               
               
                 ATGAGTTGAAGGGTGGTGTTGCAGGTGGCTCTATCCTCACTGGTGTGCTGAAGCTG 
               
               
                   
               
               
                 AACGACGAGATTGAGATTCGCCCCGGTCTCGTTACCAAGGATGAGAACGGAAAGAT 
               
               
                   
               
               
                 TCAGTGCCGCCCCATCTTCTCCCGTGTCGTTTCGCTCTTCGCTGAGCACAACGATCT 
               
               
                   
               
               
                 GAAGTTCGCTGTCCCTGGTGGTCTGATCGGTGTCGGAACCCGTGTCGACCCTACCCT 
               
               
                   
               
               
                 GTGCCGTGCCGATCGTCTCGTTGGTTTCGTCCTGGGTCACCGTGGCCGTTTGCCGGC 
               
               
                   
               
               
                 CATCTACACTGAACTGGAGGTCAACTACTTCCTCCTGCGTCGTCTGCTCGGTGTCAA 
               
               
                   
               
               
                 GACCGCCGACGGCAAGCAGGCCAAGGTCGCCAAGCTCACCAAGAACGAGGTCCTC 
               
               
                   
               
               
                 ATGGTTAACATCGGCTCTACGGCTACTGGTGCTAAGGTTATGGGTGTGAAGGCTGA 
               
               
                   
               
               
                 TGCTGCCAAGCTCAGCTTGACCAGCCCGGCTTGTACAGAGATTGGAGAGAAGATTG 
               
               
                   
               
               
                 CTATCAGCCGGAGAATTGACAAGCATTGGCGTCTGATCGGCTGGGCCAATATTGTC 
               
               
                   
               
               
                 GCTGGCAACACTCTTGAGCCCATTCTGAACTAG 
               
               
                   
               
               
                 SEQ ID NO. 79: 
               
               
                 &gt;XM_001271648.1_ Published eIF2 γ sequence for  A .  clavatus :  
               
               
                   Aspergillus   clavatus  NRRL 1 translation initiation factor 
               
               
                 EF-2 gamma subunit, putative (ACLA_046890), partial mRNA 
               
               
                 ATGGGTCATTATGAAATTGAAGAACAAGAGCCTCTTGATGTCGAGGAGAAGGCCCT 
               
               
                   
               
               
                 CAAGTCTTCGATGAAGAAGGGCTCCATGCCCCCAGTTCCTCAACCGAAGCGCCCAG 
               
               
                   
               
               
                 AACTGCCCGAGCAGCCTGACCCAGAGACCATCGATCTGTCGAAACTGACGCCTCTG 
               
               
                   
               
               
                 TCCCCCGAAATTATCGCGCGCCAGGCCACGATCAACATTGGTACCATCGGACACGT 
               
               
                   
               
               
                 CGCTCACGGCAAGTCGACCGTGGTGAAGGCTATCTCGGAGGTGCAGACGGTCCGTT 
               
               
                   
               
               
                 TCAAGAACGAGTTGGAGCGGAATATTACCATCAAGCTGGGTTATGCCAACGCCAAG 
               
               
                   
               
               
                 ATCTACAAGTGCGACAGCCCTGAGTGCCCTCGGCCGACATGCTACAAGAGTTACAA 
               
               
                   
               
               
                 GAGTGAGAAGGAGGTCGACCCTCCTTGCGAAAGAGAAGGATGCACAGGTCACTAC 
               
               
                   
               
               
                 AGACTGCTGAGACACGTTTCTTTCGTTGACTGCCCCGGTCACGACATTCTCATGAGC 
               
               
                   
               
               
                 ACTATGTTGTCAGGCGCCGCCGTCATGGACGCCGCCCTTCTTTTGATTGCCGGAAAC 
               
               
                   
               
               
                 GAAGCCTGCCCTCAGCCCCAGACCTCGGAGCACTTGGCAGCCATTGAGATCATGAA 
               
               
                   
               
               
                 GCTCAGCCACATTATCATCCTGCAGAACAAGGTCGATCTGATGAGAGAGGATGGAG 
               
               
                   
               
               
                 CTTTGCAACATTACCAGTCGATTCTGAAGTTCATCCGTGGTACTGTCGCTGATGGCT 
               
               
                   
               
               
                 CGCCCATCATTCCTATCTCTGCGCAGCTCAAGTACAACATTGATGCTGTTAACGAAT 
               
               
                   
               
               
                 ACCTTGTTTCGCACATCCCCGTCCCCGTCCGTGACTTCACTGCTTCCCCTCACATGAT 
               
               
                   
               
               
                 CGTCATCCGTTCCTTCGACGTCAACAAGCCCGGTGCGGAGATTGATGAGCTGAAGG 
               
               
                   
               
               
                 GTGGTGTTGCCGGTGGCTCTATCCTGACTGGTGTGCTCAAGTTGAATGATGAGATCG 
               
               
                   
               
               
                 AGATCCGCCCTGGTCTCGTTACCAAGGACGAGAACGGCAAGATTCAGTGCCGTCCC 
               
               
                   
               
               
                 ATCTTCTCGCGTGTTGTCTCGCTCTTTGCCGAGCACAACGACCTGAAGTTTGCTGTT 
               
               
                   
               
               
                 CCTGGTGGTCTGATCGGTGTCGGCACCCGTGTCGACCCTACTCTGTGCCGTGCTGAT 
               
               
                   
               
               
                 CGTCTCGTTGGTTTCGTCCTGGGTCACCGTGGTCGCCTGCCCGCTATTTACACTGAA 
               
               
                   
               
               
                 CTGGAGGTCAACTACTTCTTGCTGCGTCGTCTGCTCGGTGTCAAGACCGCCGATGGC 
               
               
                   
               
               
                 AAGCAGGCTAAGGTTGCCAAGCTGACCAAGAACGAGGTTCTCATGGTCAACATCGG 
               
               
                   
               
               
                 ATCGACAGCCACTGGTGCCAAGGTTATGGGTGTGAAGGCCGACGCTGCCAAGCTCA 
               
               
                   
               
               
                 GCTTGACCAGCCCTGCCTGCACAGAAATTGGCGAGAAGATTGCCATCAGCCGAAGA 
               
               
                   
               
               
                 ATCGACAAGCATTGGCGTCTGATCGGTTGGGCCAACATTGTCGCTGGTAACACTCTT 
               
               
                   
               
               
                 GAGCCTATTCTGAACTAG 
               
               
                   
               
               
                 SEQ ID NO. 80: 
               
               
                 &gt;XM_001214623.1_ Published eIF2 sequence for  A .  terreus   
               
               
                 ATGGCTACCAACGGCGATTTCACCGACGATGAATCCCAGCCCGGTTCCCCCGTCAT 
               
               
                   
               
               
                 GGAGCCCAACGGCCAGTACGACATTGAAGAACAGGAGCCTCTCGACCAGCCCCTG 
               
               
                   
               
               
                 AAGTCGGCGATGAAGAAGGACTCTGCTCTTTCCCCCCAGCCGAAGCGCCCAGAGCT 
               
               
                   
               
               
                 TCCTGAACAACCCAACCCAGACACCCTCGATCTGTCGACGCTTACCCCTCTGTCGCC 
               
               
                   
               
               
                 CGAAATTATTGCGCGCCAGGCCACCATCAACATCGGTACCATTGGTCACGTCGCTC 
               
               
                   
               
               
                 ACGGAAAGTCGACGGTTGTCAAGGCCATCTCAGAGGTCCAGACCGTTCGATTCAAG 
               
               
                   
               
               
                 AACGAGTTGGAACGGAATATTACGATTAAGCTGGGTTATGCCAACGCCAAGATCTA 
               
               
                   
               
               
                 CAAGTGCGACAACCCCGAGTGCCCTCGGCCGACTTGTTACAAGAGTTTCAAGAGTG 
               
               
                   
               
               
                 AGAAGGAGGTCGACCCGCCATGTGAGAGAGATGGCTGCACAGGTCGTTACCGTCTA 
               
               
                   
               
               
                 CTGAGACACGTCTCCTTTGTCGACTGCCCCGGTCACGATATTCTCATGAGTACCTGT 
               
               
                   
               
               
                 TGTCTGGTGCCGCCGTCATGGACGCTGCCCTTCTCCTGATTGCCGGAAACGAAACCT 
               
               
                   
               
               
                 GCCCCCAGCCTCAGACCTCGGAGCACTTGGCTGCTATTGAGATCATGAAGCTGAGT 
               
               
                   
               
               
                 CATATCATTATCCTGCAGAACAAGGTCGATCTGATGCGCGAGGACGGTGCCCTGCA 
               
               
                   
               
               
                 GCACTACCAGTCGATCCTGAAGTTCATCCGTGGTACTGTGGCAGACGGCTCTCCCAT 
               
               
                   
               
               
                 TATCCCCATCTCCGCCCAGCTGAAGTACAACATCGATGCGGTCAACGAGTACCTCG 
               
               
                   
               
               
                 TGTCGCACATCCCCGTCCCCGTCCGTGACTTTACCGCCTCTCCTCACATGATTGTCAT 
               
               
                   
               
               
                 TCGCTCCTTCGACGTCAACAAGCCCGGTGCCGAGATTGATGATCTGAAGGGTGGTG 
               
               
                   
               
               
                 TCGCTGGTGGTTCCATCCTGACAGGTGTGCTGAAGCTGAACGACGAGATCGAAATC 
               
               
                   
               
               
                 CGTCCCGGTCTGGTCACGAAGGACGAGAACGGCAAGATCCAGTGCCGTCCCATCTT 
               
               
                   
               
               
                 CTCTCGCGTGGTCTCCCTATTCGCCGAGCACAACGACCTCAAGTTCGCGTGCCCGGC 
               
               
                   
               
               
                 GGTCTTATCGGTGTTGGTACTCGCGTTGACCCTACCCTCTGCCGTGCGGATCGTCTT 
               
               
                   
               
               
                 GTTGGTTTCGTCCTGGGTCACCGTGGTCGCCTGCCTGCTATCTACACTGAGCTGGAG 
               
               
                   
               
               
                 GTTAACTACTTCTTGCTGCGTCGTCTGCTCGGTGTGAAGACCGCCGACGGAAAGCA 
               
               
                   
               
               
                 GGCTAAGGTCGCCAAGCTGGCCAAGAACGAAGTTCTGATGGTGAACATTGGATCTA 
               
               
                   
               
               
                 CGGCCACCGGTGCCAAGGTGATGGGTGTGAAGGCTGATGCTGCCAAGCTCAGCTTG 
               
               
                   
               
               
                 ACCAGCCCTGCCTGTACCGAGATCGGAGAGAAGATCGCCATCAGTCGGAGAATTGA 
               
               
                   
               
               
                 GAAGCACTGGCGTCTGATCGGTTGGGCCAACATTGTTGCCGGTAACACCCTGGAGC 
               
               
                   
               
               
                 CCATCCTGAACTAA 
               
               
                   
               
               
                 SEQ ID NO. 81: 
               
               
                 &gt;gi|169773704|ref|XM_001821269.1|  Aspergillus   oryzae   
               
               
                 RIB40 hypothetical protein partial mRNA 
               
               
                 ATGGCTGCCAACGGCGATTTTTCCGATGATGAATCCCAGCCGGGATCCCCCATGCT 
               
               
                   
               
               
                 GAATGCGAACGGCCATGATGATATTGAAGAACAAGAGCCCCTCGACCAAGAGGAG 
               
               
                   
               
               
                 AAGCCTCTCAAGTCTGCGATGAAGAGTGTACCCCCTGTTTCTCAGCCCAAGCGGCC 
               
               
                   
               
               
                 AGAGTTGCCCGAACAGCCAGACCCCGCTACCCTTGACCTGTCGACCCTGACCCCTCT 
               
               
                   
               
               
                 GTCGCCCGAAATCATTGCGCGCCAGGCCACTATTAACATTGGTACCATCGGACACG 
               
               
                   
               
               
                 TCGCTCACGGAAAGTCAACAGTGGTCAAGGCTATCTCAGAGGTTCAGACTGTCCGT 
               
               
                   
               
               
                 TTCAAAAACGAGTTGGAGCGAAACATTACAATCAAGCTGGGCTACGCCAACGCCAA 
               
               
                   
               
               
                 GATCTACAAGTGCGACAACCCCGAGTGTCCTCGCCCAACATGCTTCAAGAGTTTCA 
               
               
                   
               
               
                 AGAGTGAGAAGGAGATCGACCCTCCATGTGAGAGAGATGGGTGCACAGGACGTTA 
               
               
                   
               
               
                 TAGGCTGTTGAGACATGTCTCCTTCGTTGACTGCCCCGGTCACGATATTCTGATGAG 
               
               
                   
               
               
                 TACCATGTTGTCAGGTGCCGCCGTCATGGACGCAGCTCTTCTTCTGATTGCCGGAAA 
               
               
                   
               
               
                 CGAAACTTGCCCTCAGCCTCAAACCTCGGAACATTTGGCAGCTATCGAGATTATGA 
               
               
                   
               
               
                 AGCTTAGCCATATTATCATCTTGCAAAATAAGGTTGATCTGATGAGGGAAGAAGGA 
               
               
                   
               
               
                 GCTTTTCAGCATTACCAATCGATTCTGAAGTTCATCCGTGGTACTGTTGCTGATGGC 
               
               
                   
               
               
                 TCTCCTATTATCCCCATCTCCGCTCAGCTGAAGTACAACATTGATGCTGTCAAC 
               
               
                   
               
               
                 GAATACCTTGTTTCCCACATCCCTGTCCCTGTCCGTGATTTCACCGCTTCGCCACAC 
               
               
                   
               
               
                 ATGATCGTCATCCGTTCATTCGATGTGAACAAGCCTGGTGCCGAGATTGATGAGCT 
               
               
                   
               
               
                 GAAGGGCGGTGTTGCTGGTGGTTCCAT 
               
               
                   
               
               
                 TCTGACTGGTGTGCTTAAGCTTAACGACGAGGTGGAAATCCGTCCCGGTCTCGTAA 
               
               
                   
               
               
                 CCAAGGACGAGAACGGCAAGATTCAGTGCCGGCCCATCTTCTCGCGGGTTGTTTCT 
               
               
                   
               
               
                 CTCTTCGCTGAGCACAACGACCTGAAATTTGCTGTTCCTGGTGGTCTTATTGGTGTC 
               
               
                   
               
               
                 GGTACCCGTGTGGACCCTACTCTGTGCCGTGCCGATCGTCTTGTCGGTTTCGTCCTG 
               
               
                   
               
               
                 GGCCATCGCGGACGTCTGCCCGCCATTTACACCGAACTGGAGGTCAACTATTTC 
               
               
                   
               
               
                 TTGCTGCGCCGGTTGTTGGGTGTGAAGACCGCCGACGGCAAGCAGGCCAAGGTTGC 
               
               
                   
               
               
                 TAAGCTGAGCAAGAACGAGGTTCTGATGGTCAACATCGGTTCTACGGCCACCGGTG 
               
               
                   
               
               
                 CTAAGGTCATGGGCGTCAAGGCCGATGCTGCAAAGCTGAGCTTGACCAGCCCTGCT 
               
               
                   
               
               
                 TGTACCGAAATTGGCGAGAAGATTGCCATTTCTCGCAGAATCGACAAGCACTGGCG 
               
               
                   
               
               
                 TCTGATTGGGTGGGCTAACATTGTTGCCGGTAACACCCTCGAACCCATCCTGAACTAA 
               
               
                   
               
               
                 SEQ ID NO. 82: 
               
               
                 &gt;XM_001401525.1_ Published eIF2γ sequence for  A .  niger   
               
               
                 ATGGCTGACGATGACATCGAAGAGCAAGAGCCCCTCGACCAGGAGGCCAAGCCTC 
               
               
                   
               
               
                 TGAAGTCTGCGATGAAGAAGGAAGTTCCTCCTCCCCAGCCCAAGCGGCCAGAGCTG 
               
               
                   
               
               
                 CCCGAGCAGCCGAATCCGGAGACTCTGGACCTGTCCACCCTGACTCCTTTGACCCCC 
               
               
                   
               
               
                 GAAATTATTGCGCGCCAAGCCACAATCAACATTGGCACCATCGGTCACGTCGCTCA 
               
               
                   
               
               
                 CGGCAAGTCGACGGTCGTTAAGGCTATCTCCGAGGTCCAGACTGTCCGTTTCAAGA 
               
               
                   
               
               
                 ACGAGTTGGAGCGAAACATTACGATCAAGCTGGGTTATGCCAACGCAAAGATCTAC 
               
               
                   
               
               
                 AAGTGCGACAACCCCGAGTGCCCTAGGCCGACATGCTTTAAGAGCTTTAAGAGTGA 
               
               
                   
               
               
                 GAAGGAAGTCGACCCGCCTTGTGAGAGGGATGGCTGCGGTGGCCGCTACAGACTGT 
               
               
                   
               
               
                 TGAGACATGTGTCTTTCGTCGACTGCCCCGGTCACGATATTCTGATGAGTACTATGT 
               
               
                   
               
               
                 TGTCTGGTGCCGCCGTCATGGACGCTGCCCTCCTCCTTATTGCCGGAAACGAAACTT 
               
               
                   
               
               
                 GCCCTCAACCTCAGACTTCGGAGCACTTGGCTGCCATCGAAATCATGAAGCTCAGC 
               
               
                   
               
               
                 CACATCATCATTTTGCAAAACAAGGTGGACTTGATGAGAGAGGATGGTGCCCTGCA 
               
               
                   
               
               
                 ACATTACCAGTCGATCTTGAAGTTCATCCGTGGTACTGTCGCCGATGGCTCTCCGAT 
               
               
                   
               
               
                 CATTCCCATTTCTGCACAGCTCAAGTACAACATCGATGCTGTCAACGAATACCTGGT 
               
               
                   
               
               
                 TTCGCACATTCCCGTCCCCGTCCGCGATTTCACCGCTTCCCCCCACATGATCGTCATT 
               
               
                   
               
               
                 CGTTCCTTCGATGTGAACAAGCCTGGTGCCGAAATTGAGGAGCTGAAGGGTGGTGT 
               
               
                   
               
               
                 TGCCGGTGGTTCGATCTTGACTGGTGTTCTGAAGCAGAACGACGAGATTGAGATTC 
               
               
                   
               
               
                 GTCCCGGTCTGGTCACCAAGGACGAGAACGGCAAGATTCAGTGCCGTCCCATCTTC 
               
               
                   
               
               
                 TCTCGGGTCATGTCCCTCTTTGCCGAGCACAACGACCTCAAGTTTGCCGTCCCTGGT 
               
               
                   
               
               
                 GGTTTGATTGGTGTCGGTACTCGTGTAGACCCTACTCTGTGCCGTGCTGATCGTCTC 
               
               
                   
               
               
                 GTTGGTTTCGTCCTGGGTCACCGCGGACGCCTTCCCGCTATCTACACTGAGTTGGAA 
               
               
                   
               
               
                 GTCAACTACTTCTTGCTTCGTCGTCTGCTCGGTGTCAAGACTGCCGATGGCAAGCAG 
               
               
                   
               
               
                 GCCAAGGTTGCCAAGCTTACTAAGAACGAGGTTCTCATGGTCAACATCGGTTCTAC 
               
               
                   
               
               
                 GGCTACCGGAGCTAAGGTCGTGGGTGTCAAGGCTGATGCTGCCAAGCTCAGCTTGA 
               
               
                   
               
               
                 CCAGCCCTGCCTGTACCGAGGTCGGAGAGAAGATTGCCATCAGTCGGAGAATTGAG 
               
               
                   
               
               
                 AAGCACTGGCGTCTGATCGGTTGGGCCAACATTGTCGCTGGTAACACCCTTGAGCC 
               
               
                   
               
               
                 CATCCTGAACTAA 
               
               
                   
               
               
                 SEQ ID NO. 83: 
               
               
                 &gt;XM_656982.1_ Published eIF2γ sequence for  A .  nidulans   
               
               
                 ATGGCTACCAACGGCGATTTTTCAGACGAGGAGTCCCAGCCCGGGTCTCCCATTCTT 
               
               
                   
               
               
                 AACGCCAATGGCCAGGATGATATCCAAGACCAAGAGCCCCTCGAGCAGGAGGAGA 
               
               
                   
               
               
                 AGCCCATCAAGTCAGCGATGAAGAAGGACTTCATACCTGTTTCTCAGCCGAAACGA 
               
               
                   
               
               
                 CCGGAACTACCGGAGCAACCCAACCCAGAAACGCTCGACCTGTCTACACTAACTCC 
               
               
                   
               
               
                 TCTGTCACCTGAGATTATCGCCCGCCAGGCTACGATTAACATCGGTACCATTGGCCA 
               
               
                   
               
               
                 CGTCGCTCACGGTAAGTCAACGGTGGTGAAGGCTATTTCAGAGGTTCAAACTGTCC 
               
               
                   
               
               
                 GATTTAAGAACGAGTTGGAGCGAAACATTACCATCAAGCTGGGTTATGCCAACGCG 
               
               
                   
               
               
                 AAAATCTACAAGTGCGACAACCCCGCTTGCCCTCGGCCGACATGCTACAAGAGCTA 
               
               
                   
               
               
                 TAAGAGTGAGAAGGAAATTGATCCGCCCTGTGAGAGAGATGGATGCTCTGGCCGCT 
               
               
                   
               
               
                 ACCGTCTCTTAAGACACGTTTCCTTCGTCGACTGCCCTGGTCACGACATTCTTATGA 
               
               
                   
               
               
                 GTACCATGTTGTCAGGTGCCGCTGTCATGGATGCTGCTCTTTTGCTTATCGCTGGAA 
               
               
                   
               
               
                 ACGAAACCTGTCCTCAGCCCCAGACTTCGGAGCATTTGGCTGCTATTGAAATCATG 
               
               
                   
               
               
                 AAGCTTAGCCACATCATTATCCTTCAAAACAAGGTCGATTTGATGAGGGAAGATGG 
               
               
                   
               
               
                 AGCGTTGCAGCATTACCAGTCGATCTTGAAATTTATCCGTGGTACCGTTGCCGACGG 
               
               
                   
               
               
                 CTCTCCCATCATTCCCATCTCCGCTCAGCTCAAGTACAACATCGATGCCGTCAACGA 
               
               
                   
               
               
                 GTATCTGGTTTCGCACATCCCCGTGCCAGTCCGCGATTTCACGGCATCTCCTCACAT 
               
               
                   
               
               
                 GATTGTTATCCGGTCTTTCGACGTGAACAAGCCTGGTGCAGAGATTGATGAGCTAA 
               
               
                   
               
               
                 AGGGTGGTGTGGCTGGTGGTTCCATTTTGACTGGTGTCCTCAAGTTGAACGATGAA 
               
               
                   
               
               
                 ATCGAAATTCGACCAGGTCTCGTCACTAAGGACGAGAACGGCAAGATCCAGTGTCG 
               
               
                   
               
               
                 CCCTATCTTCTCGCGGGTTGTGTCTTTGTTTGCCGAACACAACGACCTGAAATTCGC 
               
               
                   
               
               
                 TGTCCCCGGTGGATTGATCGGTGTTGGTACTCGTGTTGACCCTACTCTTTGCCGTGC 
               
               
                   
               
               
                 CGATCGCCTGGTTGGTTTCGTCCTCGGTCACCGTGGGCGCCTTCCCGCTATCTACAC 
               
               
                   
               
               
                 AGAGCTAGAGGTCAATTACTTTTTGCTGCGCCGACTTTTGGGTGTCAAGACTGCCGA 
               
               
                   
               
               
                 CGGCAAGCAGGCCAAGGTCGCCAAGCTGGCTAAGAACGAGGTTCTCATGGTTAATA 
               
               
                   
               
               
                 TCGGCTCTACAGCTACCGGTGCGAAGGTGGTCGGTGTCAAGGCTGATGCTGCTAAG 
               
               
                   
               
               
                 CTGAGCTTGACTAGCCCAGCCTGTACTGAGGTTGGCGAGAAGATTGCCATTAGTCG 
               
               
                   
               
               
                 AAGAATTGAGAAGCACTGGCGTTTGATTGGTTGGGCCAACATTGTTGCTGGTAACA 
               
               
                   
               
               
                 CCCTCGAGCCCATTGTCAACTAA 
               
               
                   
               
               
                 SEQ ID NO: 84: 
               
               
                 ALEF2 
               
               
                 ATAATGCTCCGATCGTGCCTA 
               
               
                   
               
               
                 SEQ ID NO: 85: 
               
               
                 GlabA 
               
               
                 CAAGAGATTTCATGCTTTCTCCAC 
               
               
                   
               
               
                 SEQ ID NO: 86: 
               
               
                 ParA 
               
               
                 CGTAAACTCAATACCAGTTCCAGTC 
               
               
                   
               
               
                 SEQ ID NO: 87: 
               
               
                 TropicA 
               
               
                 TGTCAATTATATCCCAGTTCCATTGA 
               
               
                   
               
               
                 SEQ ID NO: 88: 
               
               
                 KrusA 
               
               
                 CATGTGTATGGTCAAGTCTATTCCT 
               
               
                   
               
               
                 SEQ ID NO: 89: 
               
               
                 CEF3F 
               
               
                 TCAGCCTTGGAACAC 
               
               
                   
               
               
                 SEQ ID NO: 90: 
               
               
                 CEFR1 
               
               
                 TTGGCACAGGTATGTAG 
               
               
                   
               
               
                 SEQ ID NO: 91: 
               
               
                 GlabF1 
               
               
                 TCgTgAAgACTATCCCTgT 
               
               
                   
               
               
                 SEQ ID NO: 92: 
               
               
                 GlabR1 
               
               
                 ATCGATTTCAGCACCTGG 
               
               
                   
               
               
                 SEQ ID NO: 93: 
               
               
                 ParaF1 
               
               
                 TATCgACgCCgTCAATC 
               
               
                   
               
               
                 SEQ ID NO: 94: 
               
               
                 ParaR1 
               
               
                 ATCAACgTCAgCACCAg 
               
               
                   
               
               
                 SEQ ID NO: 95: 
               
               
                 TropicF1 
               
               
                 ACATCGATGCCGTTAACC 
               
               
                   
               
               
                 SEQ ID NO: 96: 
               
               
                 TropicR1 
               
               
                 CAAGTCTTCGACATCGGA 
               
               
                   
               
               
                 SEQ ID NO: 97: 
               
               
                 KrusF1 
               
               
                 CCCAATTTCTGCTCAGTTG 
               
               
                   
               
               
                 SEQ ID NO: 98: 
               
               
                 KrusR1 
               
               
                 CACCAGGCTTATTAACATCG 
               
               
                   
               
               
                 SEQ ID NO: 99: 
               
               
                 The amplified region of interest in eIF2γ of  Candida   
               
               
                   albicans  (XM_715569.1) is underlined. (Position of  
               
               
                 the region of interest: 790-934). 
               
               
                 ATGTCATACGACGATATAGAAAATGCCACTCCTGATATTGTTATTGGGAGTACTATA 
               
               
                   
               
               
                 GAGGAACCTGAAGAAGATTACCAAGTGGAAAGTGACAATGAGTTACAAGCCGCAG 
               
               
                   
               
               
                 ACCATGAGTCATCGCAAATAAATGAAGAATCAGCCAAAGGCAAAAAGTCAGTTGC 
               
               
                   
               
               
                 ATTTACTGGATTGGATGAAGACGAGGAAAATGCAGAGGAATTGGCCAGAAAGGAG 
               
               
                   
               
               
                 TTTGAAGAAGGTGGTGGATTGCCTGAACAACCAGAAAACCCAGATTTCAATGAGTT 
               
               
                   
               
               
                 AACACCTTTATCTCCCGAGATTATCAACAGGCAAGCCACCATTAATATTGGTACCAT 
               
               
                   
               
               
                 TGGTCATGTCGCCCACGGGAAGTCTACTGTTGTCAGGGCTATCTCTGGTGTCCAGAC 
               
               
                   
               
               
                 CGTTCGTTTCAAGGATGAATTAGAAAGAAACATTACTATCAAGTTAGGTTACGCCA 
               
               
                   
               
               
                 ATGCCAAAATTTACAAATGTGATAACCCAGAGTGTCCAGAACCAGATTGTTACAGA 
               
               
                   
               
               
                 TCATTCAAATCAGATAAGGAAATAAGACCAAAATGTCAAAGAGCTGGCTGTGACG 
               
               
                   
               
               
                 GTCGCTACAAATTGTTAAGACATGTCTCTTTTGTTGATTGTCCAGGACATGATATTT 
               
               
                   
               
               
                 TGATGAGTACTATGTTGTCAGGTGCTGCCGTGATGGATGCCGCCTTGTTGTTGATTG 
               
               
                   
               
               
                 CCGGTAATGAAAGTTGTCCACAACCCCAGACTTCTGAGCATTTGGCTGCCATTGAA 
               
               
                   
               
               
                 ATTATGAAATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGA 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 CCAAGATTGATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGA 
               
               
                   
               
               
                 CGAATTGAAAGGAGGTGTTGCAGGTGGTTCTATTTTGACTGGTGTTTTTAAGATTGG 
               
               
                   
               
               
                 TGATGAGATCGAGATTAGACCTGGTATCGTCACCAAAGATGATCAAGGAAAGATTC 
               
               
                   
               
               
                 AATGTAAACCTATATTCTCGAACGTGGTTTCCTTGTTTGCTGAGCATAACGATTTGA 
               
               
                   
               
               
                 AATTTGCTGTTCCTGGTGGTTTGATTGGTGTTGGTACTAAAGTTGATCCTACGTTGT 
               
               
                   
               
               
                 GTAGGGCTGATAGATTGGTTGGTCAAGTTGTTGGTGCAAAAGGAAACTTGCCCTCT 
               
               
                   
               
               
                 ATTTACGCTGATATTGAGATAAACTATTTCCTATTAAGAAGATTGTTGGGTGTCAAA 
               
               
                   
               
               
                 ACTGAAGGTCAAAAGCAAGGTGCTAAAGTTCGTAAGTTGGAACAATCTGAAGTGTT 
               
               
                   
               
               
                 GATGGTAAATATTGGTTCTACTGCAACTGGTGCTAGAGTGGTTGCTGTTAAAGCAG 
               
               
                   
               
               
                 ATATGGCTCGTTTACAATTGACTACACCAGCCTGTACAGAAATCAACGAAAAAATT 
               
               
                   
               
               
                 GCGTTGTCTAGACGTATTGAAAAGCATTGGCGTTTGATTGGTTGGGCCACTATCAAG 
               
               
                   
               
               
                 AAAGGTACAGCATTAGAACCAATTTCTTAA 
               
               
                   
               
               
                 SEQ ID NO: 100: 
               
               
                 The amplified region of interest in eIF2γ of  Candida   
               
               
                   glabrata  (XM_447610.1) is underlined. 
               
               
                 (Position of the region of interest: 872-972). 
               
               
                 ATGTCTGATTTGCAAGATCAAGAGCCAACTATTATTATCAATGGTGATCTTCCACCA 
               
               
                   
               
               
                 GTAGAAGAAGAGGAAGTCTATGAGCAGGAAGAGCAAGAGGAAGTTGTTGAGGAGA 
               
               
                   
               
               
                 AGCCAAAGAAGAAAGTTGCCTTTACCGGTCTAGAGGATGGTGAATCTGAGGAAGA 
               
               
                   
               
               
                 GAAGAGAAAGAGAGAGTTTGAAGAAGGTGGTGGATTGCCAGAGCAGCCAGAAAAC 
               
               
                   
               
               
                 CCAGACTTTACTAAGTTGAACCCACTTTCTGCTGAGATTATTAACAGACAAGCTACT 
               
               
                   
               
               
                 ATCAACATCGGTACTATTGGTCATGTCGCTCACGGTAAGTCTACTGTTGTCAGAGCC 
               
               
                   
               
               
                 ATCTCTGGTGTCCAAACCGTTCGTTTCAAGGATGAGTTGGAACGTAACATTACTATC 
               
               
                   
               
               
                 AAGCTGGGTTATGCCAATGCTAAGATATATAAGTGTCAAGAGCCTACATGTCCAGA 
               
               
                   
               
               
                 ACCAGACTGTTACAGATCTTTCAAGTCTGACAAAGAAATTAATCCAAAGTGTCAAA 
               
               
                   
               
               
                 GACCAGGTTGCCCAGGCCGTTACAAACTTGTTCGTCACGTCTCTTTCGTCGATTGTC 
               
               
                   
               
               
                 CAGGTCACGATATTCTAATGAGTACTATGTTGTCCGGTGCCGCTGTCATGGACGCAG 
               
               
                   
               
               
                 CCTTGTTATTGATCGCCGGTAATGAATCTTGTCCACAACCTCAAACTTCTGAACATT 
               
               
                   
               
               
                 TGGCTGCCATTGAAATCATGAAGTTAAAGCACGTTATTATTCTACAGAACAAGGTC 
               
               
                   
               
               
                 GATTTAATGCGTGAAGAAAGCGCACTAGAACATGAAAAGTCTATCCTGAAATTTAT 
               
               
                   
               
               
                 CAGAGGTACTATTGCTGACGGTGCTCCAATTGTCCCAATTTCCGCTCAATTGAAATA 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 GTGTTCAAGTTGGGTGATGAGATTGAAATTAGACCAGGTATTGTCACTAAGGATGA 
               
               
                   
               
               
                 TAAGGGTAAGATCCAATGTAAGCCAATTTTCTCCAACATTGTCTCTCTATTTGCTGA 
               
               
                   
               
               
                 ACAAAATGACTTGAAGTTTGCAGTCCCAGGTGGTCTGATTGGTGTTGGTACAAAGG 
               
               
                   
               
               
                 TCGATCCTACCTTATGTAGAGCTGATCGTCTTGTCGGTCAAGTTGTCGGTGCCAAGG 
               
               
                   
               
               
                 GTCACCTACCAAGCATTTACACAGATATTGAAATCAACTACTTCCTACTGCGTCGTC 
               
               
                   
               
               
                 TATTAGGTGTTAAGACTGAGAAACAAGCCAAGGTCAGAAAGCTGGTTGCCAACGA 
               
               
                   
               
               
                 AGTTCTTATGGTTAACATTGGTTCTACTGCCACTGGTGCCCGTGTCGTTGCTGTCAA 
               
               
                   
               
               
                 GGCTGATATGGCTAGATTGCAACTAACATCCCCAGCATGTACAGAAATCAATGAAA 
               
               
                   
               
               
                 AGATTGCTCTCTCTAGACGTATTGACAAGCACTGGCGTTTAATTGGTTGGGCTACAA 
               
               
                   
               
               
                 TCAAGAAAGGTACCACTTTGGAACCAGTTGTCTAA 
               
               
                   
               
               
                 SEQ ID NO: 101: 
               
               
                 The amplified region of interest is underlined. (151-274). 
               
               
                 &gt;P-604\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  
               
               
                   C .  parapsilosis   
               
               
                 GTTGAAGCACGTTATTATTTTGCAAAACAAAGTTGATTTAATGAGAAAGGAGTCAG 
               
               
                   
               
               
                 CTTTGGAACATGAAAAGTCCATCATTCAGTTCATCAGAGGTACTATAGCTGATGGT 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 AGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 102 
               
               
                 The amplified region of interest is underlined. (140-270). 
               
               
                 &gt;T94\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  
               
               
                   C .  tropicalis   
               
               
                 GTCATKATTTTGCAGAACAAGGTCGATTTGATGAGAGAAGAATCTGCCTTGGAACA 
               
               
                   
               
               
                 TGAGAAATCCATTCTTCAATTCATCAGAGGTACTATTGCAGACAATGCTCCTATTGT 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 GCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 103: 
               
               
                 The amplified region of interest is underlined. (115-224). 
               
               
                 &gt;K573E\(EF)\(CaneIF2-F) eIF2 γ sequence generated for  
               
               
                   C .  krusei   
               
               
                 TGKTGTGATTKTACAAAATAAAGTTGATTTGATGAAGAAAGAAGCAGCTTTAGAGC 
               
               
                   
               
               
                 ACGAAAAATCTATTTTGAAGTTTATCAAGGGTACTATTGCTGATGGTGCTCCTATTA 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
               
                 TAGATCTTTCGATGTTAATAAGCCTGGTGCAGAAATTGCAGATTTGAAAGGTGGTG 
               
               
                   
               
               
                 TTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 104: 
               
               
                 EF2_7_FOW 
               
               
                 AGCCCAAGCGGCCAGA 
               
               
                   
               
               
                 SEQ ID NO: 105: 
               
               
                 EF2_8_FOW 
               
               
                 AGCCGAAGCGCCCAGA 
               
               
                   
               
               
                 SEQ ID NO: 106: 
               
               
                 EF2_5_REV 
               
               
                 GGCCTGGCGCGCAAT 
               
               
                   
               
               
                 SEQ ID NO: 107: 
               
               
                 EF2_6_REV 
               
               
                 GGCTTGGCGCGCAAT 
               
               
                   
               
               
                 SEQ ID NO: 108: 
               
               
                 A.NIG_EF2_1 
               
               
                 ATCCGGAGACTCTGGACCT 
               
               
                   
               
               
                 SEQ ID NO: 109: 
               
               
                 A.TERR_EF2_1 
               
               
                 CGACGCTTACCCCTCTGT 
               
               
                   
               
               
                 SEQ ID NO: 110: 
               
               
                 A.FLAV_EF2_1 
               
               
                 CAGACCCCGCTACCCTT 
               
               
                   
               
               
                 SEQ ID NO: 111: 
               
               
                 A.FUM_EF2_1 
               
               
                 ACGCTCACACCTCTGTC 
               
               
                   
               
               
                 SEQ ID NO: 112: 
               
               
                 EF2_9_FOW 
               
               
                 AGCCGAAGCGTCCAGAAC 
               
               
                   
               
               
                 SEQ ID NO: 113: 
               
               
                 EF2_1_fow 
               
               
                 cagccgaagcg 
               
               
                   
               
               
                 SEQ ID NO: 114: 
               
               
                 EF2_2_fow 
               
               
                 cagcccaagcg 
               
               
                   
               
               
                 SEQ ID NO: 115: 
               
               
                 EF2_3_fow 
               
               
                 agccgaagcgYc 
               
               
                   
               
               
                 SEQ ID NO: 116: 
               
               
                 EF2_4_fow 
               
               
                 agcccaagcggcc 
               
               
                   
               
               
                 SEQ ID NO: 117: 
               
               
                 EF2_5_fow 
               
               
                 agccgaagcgtcc 
               
               
                   
               
               
                 SEQ ID NO: 118: 
               
               
                 EF2_6_fow 
               
               
                 agccgaagcgccc 
               
               
                   
               
               
                 SEQ ID NO: 119: 
               
               
                 EF2_10_fow 
               
               
                 agcgccccctgctcc 
               
               
                   
               
               
                 SEQ ID NO: 120: 
               
               
                 EF2_11_fow 
               
               
                 cccgagcagcccgacc 
               
               
                   
               
               
                 SEQ ID NO: 121: 
               
               
                 EF2_1_rev 
               
               
                 cgtgtgcgacgt 
               
               
                   
               
               
                 SEQ ID NO: 122: 
               
               
                 EF2_3_rev 
               
               
                 cgtgagcgagtg 
               
               
                   
               
               
                 SEQ ID NO: 123: 
               
               
                 EF2_4_rev 
               
               
                 cgtgwgcgacgt 
               
               
                   
               
               
                 SEQ ID NO: 124: 
               
               
                 EF2_7_rev 
               
               
                 cgtgtgcgacgtgtccg 
               
               
                   
               
               
                 SEQ ID NO: 125: 
               
               
                 A.fum_EF2_2 
               
               
                 cgacgctcacacctctgtc 
               
               
                   
               
               
                 SEQ ID NO: 126: 
               
               
                 &gt;CA3345\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CCTTGGAACACGAAAAATCTATCATTCAGTTTATTAGAGGTACAATTGCCGATAAT 
               
               
                   
               
               
                 GCTCCGATCGTGCCTATTTCTGCTCAATTGAAATACAACATTGATGCAGTGAATCAA 
               
               
                   
               
               
                 TTTATTGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTG 
               
               
                   
               
               
                 ATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAA 
               
               
                   
               
               
                 AGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 127: 
               
               
                 &gt;CA16733\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CCTTGGAACACGAAAAATCTATCATTCAGTTTATTARAGGTACAATTGCCGATAATG 
               
               
                   
               
               
                 CTCCGATCGTGCCTATTTCTGCTCAATTRAAATACAACATTGATGCAGTGAATCAAT 
               
               
                   
               
               
                 TTATYGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTGA 
               
               
                   
               
               
                 TCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAAA 
               
               
                   
               
               
                 GGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 128: 
               
               
                 &gt;CA1899\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CYTTGGAACACGAAAAATCTATYATTCAGTTTATTARAGGTACAATTGCCGATAAT 
               
               
                   
               
               
                 GCTCCGATCGTGCCTATTTCTGCTCAATTRAAATACAACATTGATGCAGTGAATCAA 
               
               
                   
               
               
                 TTTATYGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTG 
               
               
                   
               
               
                 ATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAA 
               
               
                   
               
               
                 AGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 129: 
               
               
                 &gt;CA1912\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CCTTGGAACACGAAAAATCTATCATTCAGTTTATTAGAGGTACAATTGCCGATAAT 
               
               
                   
               
               
                 GCTCCGATCGTGCCTATTTCTGCTCAATTGAAATACAACATTGATGCAGTGAATCAA 
               
               
                   
               
               
                 TTTATTGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTG 
               
               
                   
               
               
                 ATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAA 
               
               
                   
               
               
                 AGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 130: 
               
               
                 &gt;CA2312\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CCTTGGAACACGAAAAATCTATCATTCAGTTTATTAGAGGTACAATTGCCGATAAT 
               
               
                   
               
               
                 GCTCCGATCGTGCCTATTTCTGCTCAATTRAAATACAACATTGATGCAGTGAATCAA 
               
               
                   
               
               
                 TTTATYGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTG 
               
               
                   
               
               
                 ATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAA 
               
               
                   
               
               
                 AGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 131: 
               
               
                 &gt;CA2688\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CCTTGGAACACGAAAAATCTATCATTCAGTTTATTAAAGGTACAATTGCCGATAAT 
               
               
                   
               
               
                 GCTCCGATCGTGCCTATTTCTGCTCAATTGAAATACAACATTGATGCAGTGAATCAA 
               
               
                   
               
               
                 TTTATTGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTG 
               
               
                   
               
               
                 ATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAA 
               
               
                   
               
               
                 AGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 132: 
               
               
                 &gt;CA2701\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CYTTGGAACACGAAAAATCTATYATTCAGTTTATTAGAGGTACAATTGCCGATAAT 
               
               
                   
               
               
                 GCTCCGATCGTGCCTATTTCTGCTCAATTRAAATACAACATTGATGCAGTGAATCAA 
               
               
                   
               
               
                 TTTATYGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTG 
               
               
                   
               
               
                 ATCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAA 
               
               
                   
               
               
                 AGGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 133: 
               
               
                 &gt;CA15640\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CCTTGGAACACGAAAAATCTATCATTCAGTTTATTARAGGTACAATTGCCGATAATG 
               
               
                   
               
               
                 CTCCGATCGTGCCTATTTCTGCTCAATTGAAATACAACATTGATGCAGTGAATCAAT 
               
               
                   
               
               
                 TTATTGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTGA 
               
               
                   
               
               
                 TCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAAA 
               
               
                   
               
               
                 GGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID NO: 134: 
               
               
                 &gt;CA1893\(EF2) eIF2 γ sequence generated for  C .  albicans   
               
               
                 ATTGAAACATGTTATTATTTTGCAAAATAAAGTTGATTTGATGAGAGAAGAATCAG 
               
               
                   
               
               
                 CCTTGGAACACGAAAAATCTATCATTCAGTTTATTARAGGTACAATTGCCGATAATG 
               
               
                   
               
               
                 CTCCGATCGTGCCTATTTCTGCTCAATTGAAATACAACATTGATGCAGTGAATCAAT 
               
               
                   
               
               
                 TTATTGTTAACTACATACCTGTGCCAATGAGAGACTTTACTGCTTCACCAAGATTGA 
               
               
                   
               
               
                 TCGTTATCAGATCTTTCGATGTGAACAAGCCTGGTGCAGATGTAGACGAATTGAAA 
               
               
                   
               
               
                 GGAGGTGTTGCAGGTGGTTC 
               
               
                   
               
               
                 SEQ ID No: 135: 
               
               
                 CEF1F 
               
               
                 ATCTATCATTCAGTTTATTAGAG 
               
               
                   
               
               
                 SEQ ID NO 136: 
               
               
                 CEF2F 
               
               
                 CATTCAGTTTATTAGAGGTAC 
               
               
                   
               
               
                 SEQ ID NO 137: 
               
               
                 CEFR2 
               
               
                 CAGTAAAGTCTCTCATTG 
               
               
                   
               
               
                 SEQ ID NO 138: 
               
               
                 ALEF1 
               
               
                 TGCCGATAATGCTCCGATC 
               
             
          
         
       
     
       References 
       [0142]    Alone P V, Dever T E.
       Direct binding of translation initiation factor elF2gamma-G domain to its GTPase-activating and GDP-GTP exchange factors eIF5 and eIF2B epsilon. J Biol Chem. 2006 May 5; 281(18):12636-44. Epub 2006 Mar. 7.       
 
         [0144]    Dorris D R, Erickson F L, Hannig E M.
       Mutations in GCD11, the structural gene for eIF-2 gamma in yeast, alter translational regulation of GCN4 and the selection of the start site for protein synthesis. EMBO J. 1995 May 15; 14(10):2239-49       
 
         [0146]    Erickson F L, Harding L D, Dorris D R, Hannig E M.
       Functional analysis of homologs of translation initiation factor 2gamma in yeast. Mol Gen Genet. 1997 Feb. 27; 253(6):711-9.       
 
         [0148]    Erickson F L, Hannig E M.
       Ligand interactions with eukaryotic translation initiation factor 2: role of the gamma-subunit. EMBO J. 1996 Nov. 15; 15(22):6311-20.