Abstract:
Methods and apparatus are disclosed herein to improve on and expand the range of electrical and electromagnetic frequencies used in therapeutic electro medical devices. The present invention uses electrical and electromagnetic frequency generators and detectors integrated with a live cell imaging system that provides feedback to the frequency generators using data derived from said imaging system.

Description:
FIELD OF THE INVENTION 
     The present invention relates to methods and apparatus intended to accelerate the healing rates of hard and soft human or animal tissues and promote regeneration of damaged organs using electrical and electromagnetic stimulation. 
     BACKGROUND OF THE INVENTION 
     In every arena of medical practice, the healing of tissues is the primary problem that must be dealt with. Trauma induced contusions; abrasions, organ failures, and bone damage are medical issues dealt with by the millions daily. Typical medical approaches to trauma include stitches, bandages, casts, as well as simple and complex mechanical restructuring, then letting the body takes its natural healing course. Disease is rampant in current society as evidenced by the flood of “pills” offered on television and pharmacy shelves. The bulk of these products rarely heal the tissues themselves, but seek to neutralize the symptoms resulting in side effects that are often worse than the disease itself. Non-invasive tissue regeneration tools that have no side effects are needed. 
     Key prior art patents that relate to the present invention are presented herein with summaries of their abstracts. The Yoshida et al. U.S. Pat. No. 5,922,209 describes a process for deactivating or destroying microorganisms by applying electrical energy to a microorganism through a liquid, gas or solid having electrical energy to cause an increase in an electric charge in excess of the limit of intracellular and extracellular electrostatic capacity possessed by the microorganism, which in turn results in an irreversible change in the microorganism cells and/or explosively destroys the border membrane of the microorganism cells. 
     Chang&#39;s U.S. Pat. No. 5,304,486 discloses a method of and apparatus for cell portion and cell fusion using radiofrequency electrical pulses. The method can be used to fuse or porate a variety of cells including animal cells, human cells, plant cells, protoplasts, erythrocyte ghosts, liposomes, vesicles, bacteria and yeasts. The method can also be used to produce new biological species, to make hybridoma cells which produce animal or human monoclonal antibodies and to insert therapeutic genes into human cells which can be transplanted back into the human body to cure genetic diseases. 
     The Saban, et al. U.S. Pat. No. 6,790,341 provides microband electrode array sensors for detecting the presence and measuring the concentration of analytes in a sample. The microband electrodes of the invention have both a width and thickness of microscopic dimensions. Preferably the width and thickness of the microband electrodes are less than the diffusion length of the analyte(s) of interest. The electrodes are separated by a gap insulating material that is large enough that the diffusion layers of the electrodes do not overlap such that there is no interference and the currents at the electrodes are additive. 
     Edwards, et al. U.S. Pat. No. 5,472,441 discloses a device for treating body tissues containing cancerous cells or non-malignant tumors with RF ablation, alone or in combination with systemic or localized chemotherapy. 
     The Harris, et al. U.S. Pat. No. 6,400,487 teaches methods and apparatus for screening large numbers of chemical compounds and performing a wide variety of fluorescent assays, including live cell assays. The methods utilize a laser linescan confocal microscope with high speed, high resolution and multi-wavelength capabilities and real time data-processing. 
     Chang&#39;s U.S. Pat. No. 8,278,629 discloses live-cell observation equipment for a non light-transmitting microscope to study temperature-dependent events and method thereof. 
     Hofmann&#39;s U.S. Pat. No. 4,561,961 describes a cooled microscope slide and electrode apparatus for use in live cell fusion system employing tubular electrodes so fluid may be pumped through the electrodes to dissipate heat to enhance the yield of viable hybrids. An alternate embodiment sandwiches a gasket and parallel tubular electrodes between glass slides to permit cell fusion in a closed sterile environment. 
     This inventor&#39;s own Letovsky U.S. Pat. No. 6,825,792 discloses a frequency based missile detection and neutralization system that uses some similar components and creates some similar effects in non-organic compounds as the present invention does in organic compounds. This inventor&#39;s published patent application Letovsky 20110001064—as well as the parent patent from which it is a divisional—discloses aspects related to the present invention without including the camera image data to waveform generator feedback loop, the variable color source, direct contact electrical to cell system, and non contact electromagnetic frequency application components specification provided herein which are necessary to make the present invention function as intended. 
     The present invention is the result of many years of research into electrical and electromagnetic stimulation of cells to promote accelerated healing, the potential reactivation of stem cell activity, hard and soft tissue regeneration, as well as immune system stimulation to combat cancer, heart disease, and general autoimmune dysfunction. 
     SUMMARY OF THE INVENTION 
     The primary objective of the present invention is to expand the catalog of frequencies used in the electro medical tool industry by providing a research toolset to observe and affect live cells and live tissue samples in real time with high resolution, high contrast live cell imaging analysis under both direct electrical contact and non-contact electromagnetic wave stimulation. The present invention may provide better frequency choices to enhance the healing processes in virtually every cell type in human and other animal bodies. 
     Another objective of the present invention is to define electrical and electromagnetic frequencies that may promote the enhanced uptake of beneficial drugs, vitamin and mineral compounds in a living organism. 
     Radiation from the sun, space, and the atmosphere, as well as brainwaves, bioelectric signals, water, and food chemistry drive living cell metabolic processes. When an organism&#39;s metabolism is out of balance with its baseline genetic programming, disease is the result. The electro medical tool industry uses electrical and electromagnetic frequencies from several hertz to light waves to assist in this metabolic rebalancing. The industry is now into its second century of evolution having begun largely in Eastern Europe. 
     Russian documentation detailing electrical physiotherapies goes from present day back to the mid 1800s, and incorporates sound, ultrasound, radio frequencies and specific light frequencies. Japanese carbon arc light healing tools date back to the second world war—healing radiation burn victims after atomic bombs were dropped on Hiroshima and Nagasaki. These highly specific carbon compounds create intense light outputs designed to reproduce specific combinations of light wave frequencies to expedite the body&#39;s natural healing processes. Clinical documentation of the effectiveness of this technology is very broad. 
     Scientists at the University of Alberta in Canada have successfully regenerated teeth from the root up—by the application of specifically configured 1.5 megahertz pulses. Electrical bone growth stimulation is now common throughout the world using specifically configured frequencies at 76.4 hertz, 40 kilohertz, and 1.5 megahertz to increase the speed of bone growth after a fracture or surgery. 
     Therapeutic “cold” lasers have been proven to increase cell metabolism, increase collagen synthesis for increased healing of soft tissues, increase osteoblast production for increased healing of bone, increase circulation through increased formation of new capillaries by release of growth factors, increase T-cell production for increased immune function, increase production of neurotransmitters such as endorphins, serotonin, ACTH etc., and increase chronic pain threshold through decreased C-fiber activity. 
     Cancer cure rates with chemotherapy and radiation—though increasing significantly in certain types of cancers—are still lacking in long term cancer cell elimination after decades of research and billions of dollars spent. Electro therapy tools in global research centers are starting to gain traction as being potentially useful for targeting cancer cells while leaving the immune system and neighboring healthy cells undamaged. 
     In the US, electro medical therapy home use products are now showing up everywhere—mostly “copy catting” each other with a very small number of frequencies used in therapeutic ultrasound, therapeutic lasers, TENS machines, galvanic skin stimulators, frequency specific microcurrent generators, etc. Often, beneficial results of these tools are “hit or miss” with users—yet scores of these product offerings in the market. In general the electro therapy tools available to the public are limited in their accuracy and effectiveness due to a lack of direct testing on live cells both inside and outside living bodies due to a lack of combined observation, analysis, and affectation tools The present invention is designed to fill this need. 
     All living cells are electrically active. Heart cells grown outside a body in-vitro will all synchronize and beat together even though they are not part of a complete heart. Cells operate and communicate with other cells both electrically and chemically. Live cells can be effected by electric stimulation as in heart pacemakers. Cells also emit electrical frequencies and voltages that can be measured with tools like electroencephalographs and electrocardiographs. Neural tissues generate oscillatory activity in many ways, driven either by mechanisms localized within individual neurons or by interactions between neurons. An electric eel can discharge electrical bursts up to 600 volts at lethal currents. 
     The present invention integrates wideband frequency generators, transducers, sensors, frequency analyzers, wavelength meters, light power meters, cameras, computers, and computer software to discover “bioactive frequencies” through computer image analysis of the effects of electrical and electromagnetic frequencies on living tissues to promote accelerated healing. 
     The present invention also incorporates a database with fields and lookup tables populated from tissue and cell reactions data derived from real time image capture and event tracking software that locks, on to cell behavior changes in response to electrical and electromagnetic frequencies applied to the cells. These datasets are continually updated as effects are observed and quantified, and the electrical and electromagnetic frequencies are automatically modified and augmented to accelerate beneficial changes. For example, If a certain frequency clearly speeds up cell division during a frequency sweep, that frequency may be locked in, and harmonics of that frequency at different points in the electromagnetic spectrum may be added with the goal of further accelerating beneficial changes in cell behavior while reducing the electrical power required. 
     In the present invention, frequencies ranging from DC to x-rays—including sub nanometer resolution monchromated light—are applied to live cell samples for both observation and affectation. Both non contact electromagnetic field generating transducers in close proximity to a live cell sample, and direct electrical application to live cell samples through electrodes fitted to microscope slides are used to apply the frequencies to the cells. The electrode fitted microscope slides may incorporate multiple electrical contact zones to apply voltage and current at various frequencies to live cell samples, read the changes in the impedance of the samples, and read the characteristics of the frequency waves (sine, square, etc.) passing through the samples. There may be a minimum of three contact points per zone—positive in, positive out, and ground—with all zones able to be wired in parallel or dealt with separately. The voltage levels may be quite high—up to 400 volts—but the current may be small—microamp to milliamp levels. Each zone may be 20 by 20 microns or smaller. This may be achieved with clear conductive overlays like cell phone touch screen flexible conductive films affixed to microscope slides. 
     Impedance matching may be necessary to correctly apply the required frequency and energy level outputs to induce desired results since tissue density and impedance changes with body depth and any electrical or electromagnetic frequency applied. Tissue simulators are used in ultrasound transducer calibration and may also be used in the present invention to set benchmarks for signal amplitude output ratios and impedance matching to account for the differences in signal penetration between a fully functional living body and a sample containing just a few living cells. 
     A more complete understanding of the present invention, as well as further features and advantages, will be obtained by reference to the following detailed description and drawings. Preferred embodiments of the present invention will be described in the dependent claims. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1  is a schematic key component diagram illustrating an electro medical tool optimization system per the present invention. 
         FIG. 2  is a slide per the present invention fitted with conductive traces contacting a cell sample. 
         FIG. 3  is a microscope head per the present invention fitted with transmission and pickup coils, as well as a conductive trace fitted slide. 
         FIG. 3A  is a microscope head per the present invention fitted with a waveguide, as well as a conductive trace fitted slide. 
         FIG. 4  is a cell sample having been affected by an electromagnetic frequency per the present invention. 
         FIG. 5  is a detail of a conductive trace pattern that may be applied to microscope slide per the present invention. 
         FIG. 6  is a series of simulated accelerated cell changes with cell perimeter and detail mapping per the present invention. 
         FIG. 6A  is a series of simulated accelerated cell changes with cell perimeter and detail mapping as well as cell uptake of a chemical compound per the present invention. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The preferred embodiment of an electro medical tool optimization system in accordance with the present invention per the system flow chart schematic as shown in  FIG. 1  may provide the capability to detect a specific live cell  15  anywhere in a live cell sample  34  containing multiple cells, and subsequently apply electrical or electromagnetic signals to a cell sample  34  and modify the behavior of any specific cell  15 . Said electrical or electromagnetic signals may be drawn from a frequency range including DC through x-rays. The present invention is designed to effect live cells, observe and collect data on said effects, and use said data to optimize said effects to catalyze a specific cell function modification or neutralization. 
     The preferred embodiment of an electro medical tool optimization system in accordance with the present invention is shown in  FIG. 1  through  FIG. 7 , and all said Figures with duplicate, expanded, or detailed renderings of specific elements will share the same numbers for said elements. In  FIG. 1  specifically, cell sample  34  and cell  15  are represented simultaneously below lens  18  and as part of a screen image in video display  60 . 
     As presented in  FIG. 1 , the present invention may include a microscope  10 , which may be configured with a monochromator  12  capable of splitting a white light source  14  into single nanometer or sub nanometer wavelengths  16  that may range from 200 to 1100 nanometers, but said wavelengths  16  in practice may only be limited by the state of the art in monochromator  12  technology. Monochromator  12  may output any of said wavelengths  16  through any cell  15  in a cell sample  34  and on through a lens  18  of microscope  10 . 
     Monochromator  12  may be operated manually or it may be electrically coupled to computer  62  which is configured with software program  63 . Software program  63  may be configured to direct monochromator  12  to provide desired specific wavelengths  16 . 
     Alternatively, software program  63  may be configured to control a variable color light source  24  that is electrically coupled to a computer  62  and capable of outputting any one of millions or billions of colors  17 , said colors  17  in practice may only be limited by the state of the art in computer software and variable color light source  24  technology. Source  24  may also be configured to output said colors  17  through any cell  15  in a cell sample  34  and on through a lens  18  of microscope  10 . Source  24  may be a video projector, an RGB color laser array, a super continuum laser, or any other variable color emitting light source that derives its color output commands manually by a user or from a software program  63  on a computer  62 . A dual beam combiner  11  may also be used to combine and aim the light outputs from monochromator  12  and source  24  through any cell  15 . 
     A waveform generator  26  with at least two channels of signal outputs  28  and  30 , may be electrically coupled to a wideband power amplifier  27  input  29  through waveform generator output  28 . Amplifier  27  output  31  may be electrically coupled to an electromagnetic field output transducer  32  which may be mechanically mounted to microscope  10  to surround a microscope lens  18  to induce an electromagnetic field into any cell  15  in cell sample  34  placed on a microscope stage  35 . In certain cases, waveform generator  26  may be also be electrically coupled directly to electromagnetic field output transducer  32  through waveform generator output  28  by bypassing amplifier  27 . Electromagnetic field output transducer  32  may be single transducer or a plurality of transducers configured to generate an electromagnetic field in response to any waveform  9  provided by waveform generator  26 . In certain frequency ranges electromagnetic field output transducer  32  may be a waveguide as shown in  FIG. 3A . 
     Waveform generator  26  may output any standard function generator waveform  9  including but not limited to sine, square, ramp, pulse, noise, DC, as well as a user defined waveform  9  with sweep functionality, variable duty cycle, variable amplitude , and variable frequency range from DC to 300 gigahertz ideally—which is where infrared light frequencies begin. The Anritsu MG3690C with frequency extender options can cover near DC to 500 gigahertz in one box. For the purposes of the present invention said waveform generator  26  frequency range in practice may only be limited by the current state of the art. 
     Waveform Generator  26  is also connected to computer  62  through instrument interface  76  to allow data transfer and functional control by software program  63 . All components presented herein are connected to computer  62  through instrument interface  76  for bidirectional data transfer. These data couplings between computer  62  and all other components described in the present invention may be USB, RS232, Firewire, GPIB, or any other industry standard instrumentation data interface. The electrical signal couplings between all components may be BNC cables or any other industry standard. 
     Power amplifier  27  should have frequency response equal to waveform generator  26 . waveform generator  26  may also have an input  25  which is electrically coupled to computer  62 . Electromagnetic field output transducer  32  should have frequency response equal to waveform generator  26 . 
     The input  37  of a wideband amplifier  40  may be electrically coupled to an electromagnetic pickup transducer  42  which may be mechanically mounted concentrically to transducer  32  on microscope  10  such that it can detect any electromagnetic waves that may be applied to the proximity of any cell  15  in cell sample  34  by electromagnetic transducer  32 . Amplifier  40  ideally may eliminate any impedance mismatches in pickup transducer  42  as any waveform  9  may be applied to transducer  32  from waveform generator  26  through power amplifier  27 . Amplifier  40  may have the same frequency response as amplifier  27 . Electromagnetic pickup transducer  42  may be a single transducer or a plurality of transducers configured to sense an electromagnetic field in response to any waveform  9  provided by waveform generator  26 . In certain frequency ranges electromagnetic pickup transducer  42  may be a waveguide as shown in  FIG. 3A . 
     An oscilloscope  36  input channel  33  and a spectrum analyzer  38  input channel  35  may be electrically coupled in parallel to the output  39  of amplifier  40 . Oscilloscope  36  and spectrum analyzer  38  may have the same frequency response as waveform generator  26  and may be used to insure that any waveform  9  applied to transducer  32  are in fact reaching cell sample  34 . Oscilloscope  36  and spectrum analyzer  38  may also be directly electrically connected to transducer  42  and in certain configurations of the present invention by bypassing amplifier  40 . Additionally, oscilloscope  36  and spectrum analyzer  38  functions may be incorporated into a single spectrophotometer device. 
     In the preferred embodiment of the present invention in  FIG. 1 , waveform generator  26 , wideband power amplifier  27 , microscope stage  35 , wideband amplifier  40 , oscilloscope  36 , spectrum analyzer  38 , wideband power amplifier  48 , wideband amplifier  52 , camera  58 , wavelength meter  70 , and optical power meter  72  may all be electrically coupled to computer  62  through industry standard instrumentation interface  76  to allow functional control by software program  63 . 
     As detailed in  FIG. 2 , cell sample  34  may also be contained on an electrically conductive microscope slide  44 . Slide  44  may be configured with a minimum of three electrically conductive traces  45 ,  46 , and  47  configured to make electrical contact with any cell  15  in cell sample  34 . 
     Referring again to  FIG. 1 , slide  44  and traces  45 ,  46 , and  47  are shown expanded on display  60  to further clarify their configuration. Trace  46  may be the ground connection for input trace  45  and output trace  47 . Slide  44  input trace  45  may be electrically connected to wideband power amplifier  48  output  49 . Input  50  on amplifier  48  may be electrically connected to output  30  of waveform generator  26 . Power amplifier  48  should have frequency response equal to waveform generator  26 . 
     Slide  44  output  47  may be electrically connected to input  51  of a wideband amplifier  52 . Amplifier  52  output  53  may be electrically coupled in parallel to oscilloscope  36  second input channel  55  and spectrum analyzer  38  second input channel  56 . Amplifier  52  ideally may eliminate impedance mismatches in any cell  15  in a cell sample  34  as any waveform  9  may be applied to traces  45  and  47  from waveform generator  26  through power amplifier  48 . Amplifier  52  should have the same frequency response as waveform generator  26 . 
     Camera  58  may be mounted on a beam splitter  81  on said microscope  10  so as to view cell sample  34  through lens  18 . A video display  60  input  61  may be coupled to video output  59  of camera  58  to allow a user to monitor any effect of waveform generator  26  waveform  9  on any cell  15  in a cell sample  34 . Additionally, video output  59  may be also electrically coupled to computer  62  through instrument interface  76 . Software program  63  may be configured to track and map any. cell  15  in cell sample  34  and populate database  64  with cell  15  size and cell mechanics data derived from image data provided by camera  58 . 
     Baseline cell behavior information  68  may be included in database  64 . Baseline cell behavior information  68  may include typical cell size for a given cell type, rate of mitosis, molecular pathway openings and closings relative to certain chemical compounds, etc. Software program  63  may be configured to detect any cellular behavior alteration  69  from camera  58 . Cellular behavior alteration  69  may include any cell  15  behavior deviation from a baseline cell behavior information  68 , including such changes as size and shape. Baseline cell behavior information  68  may be refreshed with respect to initial cell  15  size data at the start of every experiment. 
     Wavelength meter  70  and optical power meter  72  may also-be installed on beam splitter  81  on microscope  10  to log the spectral information of a cell sample  34  before and after application of said waveforms  9  from waveform generator  26 . Wavelength meter  70  and optical power meter  72  may be electrically coupled to computer  62  through instrument interface  76  and information they provide may continually populate relevant fields in database  64 . Software program  63  may be configured to modify the waveforms  9  in response to any cellular behavior alteration  69  occurring in response to any waveform  9 , light wavelength  16 , or light color  17 . Wavelength meter  70  and optical power meter  72  may have the same frequency response as monochromator  12  and source  24 . Wavelength meter  70  and optical power meter  72  may also be a single spectrometer device incorporating the functions of both. 
     As camera  58  image data updates database  64 , cellular behavior alteration  69  data may enable software program  63  to track hundreds of cells in a cell sample  34  simultaneously. 
     In database  64 , each cell  15  location within cell sample  34  on slide  44  may be represented in the x/y/z axes relative to a “zero” point on a three dimensional environment model mapped to the observable area of a microscope slide  44  in database  64  at a resolution of 0.2 microns, or a resolution only limited by current state of the art in optical lens technology. This type of “object of interest” microscopic targeting and tracking software is now available from an array of software providers. 
     Each cell  15  initial size may be logged with the same resolution of 0.2 microns, or a resolution only limited by current state of the art in optical lens technology. 
     Any cellular behavior alteration  69  data may be logged and updated in real time continually updating field data in database  64  as managed by software program  63 . 
     An array of statistical outputs from cellular behavior alteration  69  data may include:
     a. real time updated position information of any cell  15  in environmental model  71 .   b. acceleration/deceleration of any cell  15  in real time and over time.   c. expansion/contraction of any cell  15  in real time and over time.   d. ambient fluid flow into/out of any cell  15  in real time and over time.   

     A user of the present invention may initially set waveform generator  26  to sweep a waveform  9  from DC up to the frequency limits of waveform generator  26  at a particular sweep rate not to exceed the image and data acquisition limits of camera  58 . When any cellular behavior alteration  69  occurs, the waveform  9  being output at that moment may be locked in by a user or software program  63  and the signal amplitude may be raised or lowered or the pulse width or duty cycle may be altered. Waveform generator  26  may then be directed by a user or software program  63  to add a harmonic  13  of root waveform  9  to waveform  9 . Additional harmonics from  13   p  to  13   n , as well as wave shape and amplitude alterations may also be tested until no more cellular behavior alteration  69  occurs. 
     A user may also manually choose to lock-in or sweep through the light wavelength  16  outputs of monochromator  12  and variable color source  24  colors  17 . Software program  63  may also be configured to sweep through and lock in any wavelength  16  or colors  17  of light, or combinations of the light outputs of monochromator  12  and variable color source  24  at a particular sweep rate not to exceed the image and data acquisition limits of camera  58 . 
     Those frequency waves that are most absorbed by a cell  15  may be considered for the purposes of the present invention as indicated in  FIG. 6 , resonant frequencies  8  of a cell  15 . Said resonant frequencies  8  are a subset of any available waveform  9  and must initially be identified through laboratory experimentation, and are then integrated within database  64  as lookup tables. “Overdriving” the amplitude of said resonant frequencies  8  with respect to a base rate of frequency absorption of a cell  15 , said rate data contained in database  64 , may affect the electrical conductivity and the chemical and mechanical conditions of a cell  15 . These resonant frequencies  8  may then be manipulated and augmented by changes in pulse rate, amplitude, and wave shape, as well as the addition of frequency inversions, harmonics, and dissonances of said resonant frequencies  8  by software program  63  through computer  62  and waveform generator  26 . It may be the manipulation of these resonant frequencies  8 , combined with other waveforms  9  and one or more of harmonic  13  through  13   n  combinations that catalyze a cellular behavior alteration  69 . 
     Resonant frequencies  8 , combined with other waveforms  9  and harmonic  13  through  13   n  may be applied to catalyze destruction of a cancerous cell  15  mechanics—initially by altering a single specific structure or behavior within a cell  15 , and then outputting and altering additional applied waveforms  9  and harmonic  13  through  13   n  combinations, which may then propagate state changes in other cell  15  structures and mechanics like a domino effect—possibly allowing an immune system to recognize a cancer cell  15  within a cell sample  34  as an invader and dispatch white blood cells to destroy it For example, if a molecule of a given component may be comprised of ten atomic elements arranged in a particular way, modifying the polarity of the third most abundant atomic element in the molecule may have such a catalyzing effect on a cell  15  mechanics. 
     Referring now to  FIG. 4  which is a photograph from a laboratory experiment using the present invention, sine wave frequencies of 727 hertz were applied to a cell sample  34  consisting of residue from a wine tank. Said cell sample  34  contained many bacteria and yeast cell  15  examples in large numbers. After a few minutes it was observed that almost a cell  15  elements migrated to the cover slip edges of slide  44 —leaving the middle of the cover slip area almost empty. This is a simple example of electromagnetic effects on organisms at the microscopic level. 
       FIG. 5  is one possible trace detail per the present invention that may be rendered on a clear conductive film which may be applied to a glass or polycarbonate microscope slide. Said clear conductive film traces  45 ,  46 , and  47  may be printed or separated by conductivity neutralized areas. 
     Referring now to  FIG. 6 , a resonant frequency  8 , individually or combined with a waveform  9  and a harmonic  13  through  13   n  combinations may be applied to a cell  15  through traces  45  and  46  and/or electromagnetic transducer  32  to stimulate a cellular behavior alteration  69  in the form of simulated accelerated mitosis as seen in the series of cell  15  changes illustrated in the top row. In the bottom row of  FIG. 6 , said cellular behavior alteration  69  may be mapped (by software program  63  in response to image data derived from camera  58  as detailed in  FIG. 1 ) as black outline  80  around the perimeter of cell  15  and black outline  82  around the nucleus  83  of a cell  15 . The black arrows indicate electromagnetic waves radiating from electromagnetic transducer  32 . 
     Referring now to  FIG. 6A , a catalytic bioavailable media  78  which may include a drug, a vitamin, or a mineral compound, in conjunction with a resonant frequency  8 , individually or combined with a waveform  9  and a harmonic  13  through  13   n  combinations may be applied to a cell  15  through traces  45  and  46  and/or electromagnetic transducer  32  to stimulate a cellular behavior alteration  69  in the form of simulated accelerated mitosis as seen in the series of cell  15  changes illustrated in the top row. In the bottom row of  FIG. 6A , said cellular behavior alteration  69  may be mapped (by software program  63  in response to image data derived from camera  58  as detailed in  FIG. 1 ) as black outline  80  around the perimeter of cell  15  and black outline  82  around the nucleus  83  of a cell  15 . The black arrows indicate electromagnetic waves radiating from electromagnetic transducer  32 . 
     Many of the elements and software capabilities included in the present invention are available in various industries and disciplines so they are not detailed herein beyond the description presented. However, the present invention is a unique and novel system and apparatus combination that incorporates methods, features, and components not found in any other single apparatus or toolset. 
     It is to be understood that the embodiments and variations shown and described herein are merely illustrative of the principles of this invention and that various modifications may be implemented by those skilled in the art without departing from the scope and spirit of the invention.