Abstract:
A method for locating pattern matches in amino acids by use of various and sequential filters capable of determining inner sample pattern matches, inner group pattern matches, and word matching for purposes of further analysis or data mining. Filters include the use of a scoring scheme, comparison of scan numbers versus sequence of common ions to be MS/MS, and daughter ion subtraction for obtaining pattern match candidates.

Description:
FIELD OF THE INVENTION 
     This invention relates generally to methods for determining amino acid composition, and more specifically, to sequence discovery between sample groups through a method of detecting matching patterns. 
     BACKGROUND INFORMATION 
     The use of mass spectrometers, including those which perform tandem mass spectrometry (MS/MS), are known to be useful in determining amino acid sequences. Currently available spectrometers are capable of presenting a wide mass range of ions, MS/MS providing daughter ion analysis of a single parent mass. The parent ion is selected by use of a frequency of applied supplemental voltage and the daughter ions are ejected by scanning. From this it is possible to obtain a reconstructed parent ion spectrum from a large number of successive daughter ion scans. Parent ions may also be excited by pulses of energy at their resonant frequency, the daughter ions may be ejected from the ion trap at their resonant frequency. The available means for determining unique amino acid sequence lacks specificity. 
     Ion trap mass spectrometers, such as those manufactured by the Finnigan Corporation, employ an electrostatic field in which ions are formed and stored by use of a quadrupole trapping field. In a tandem quadrupole ion trap mass spectrometer, ions are formed and stored in an ion trap and then mass selected by a mass analyzer wherein the ions are dissociated by means of a collision induced dissociation with a gas or surface and the fragment ions are analyzed by means of a mass or energy analyzer. During the operation of a tandem quadrupole ion trap mass spectrometer product ions, or daughter ions, are produced by isolating a precursor ion, or parent ion, which has undergone the collision induced dissociation. The collision induced dissociation can occur by applying an excitation waveform between end cap electrodes. A radio frequency voltage generator can be used to supply an RF voltage between the end caps and a ring electrode which provides the quadrupole field for trapping ions. A supplemental RF generator is coupled to the end caps to resonate trapped ions at their axial resonant frequencies. These excited ions undergo fragmentation upon the colliding with a surface or buffer gas, such as helium, placed within the buffer trap. An end cap is perforated to allow unstable ions in the field of the ion trap to exit and be detected by an electron multiplier. The magnitude and/or frequency of the fundamental RF voltage can be varied for providing mass selection. The goal is to isolate a particular ion. 
     Commercially available quadrupole ion trap mass spectrometers capable of tandem mass spectrophotometry are capable of isolating a single parent ion by inducing a collision dissociation to produce daughter ions which are resonantly ejected from an ion trap for detection by a mass-selective instability scan. Alternative techniques that should also be noted include the application of simultaneous excitation voltages or waveforms across the end caps. 
     After any characteristic daughter ions in the trap have been ejected, application of a parent ion resonant excitation waveform, can result in some parent ions that are resonantly ejected resulting in false positive readings during the detection of the characteristic daughter ions. 
     Sample mass may be analyzed by any combination of ionization and mass spectrometer. The ionization method may include, but is not limited to such methods as electron ionization, chemical ionization, fast atom bombardment, desorption chemical ionization, plasmadeorption, thermospray, atmospheric pressure chemical ionization, MALDI or electrospray ionization (ESI). Principally the ionization method will be MALDI or ESI. Prior art mass spectrometer formats are known to be useful either singly or in various combinations for use in analyzing translation products. These formats may include, but are not limited to, ionization (I) techniques, including but not limited to matrix assisted laser desorption (MALDI), continuous or pulsed electrospray (ESI) and related methods (e.g., IONSPPAY or THERMOSPRAY), or massive cluster impact (MCI); these ion sources can be matched with detection formats including linear or non-linear reflection time-of-flight (TOF), single or multiple quadropole, single or multiple magnetic sector, Fourier Transform ion cyclotron resonance (FTICR), ion trap, and combinations thereof (e.g., ion-trap/time-of-flight). For ionization, numerous matrix/wavelength combinations (MALDI) or solvent combinations (ESI) can be employed. 
     With regard to the mass spectrometer, per se, there are several types of tandem mass spectrometers that could be used to generate the patterns of MS/MS spectra that are the substrate for the program. These mass spectrometers generate both parent ions in the +1 (MALDI and ESI) and the +2, +3, +4 etc charge state (ESI). The program takes the charge state of the parent into consideration as part of the data associated with the parent ion. The types of mass analyzer mass analyzers include the quadropole, octopole, ion trap or QUISTOR, time of flight (TOF) Time of Flight with reflectron, the Fourier transformed ion cyclotron resonance (FTICR also called FTMS), magnetic and electromagnetic sector. 
     The tandem mass spectrometer mass consist of the homogenous or heterogenous combination of two or more of any of these devices in any order (Hybrid instrument). The fragmentation method may include post source decay of the parent ion or its fragment, or collision-activated or collision-induced decomposition of the parent ions, or fragment ions, or metastable ions with a relatively immobile target such as gas molecules or other parent or fragment ions, or photons or electrons or solid objects. The combinations may preferentially include the quadropole or octopole ion trap, the Quadropole-Quadropole (DC and rf or rf only)-TOF, the ion trap-FTMS, the TOF-TOF, the ion trap-TOF, or the TOF-PSD-TOF. The signal resulting from the instrument may be mathematically transformed or filtered. 
     In view of this, sequence determination of an unknown protein or peptide from mass spectral data can be difficult due to the voluminous number of possible sequences consistent with the molecular weight of the amino acid. Multiple samples result in readings that are unacceptable for most applications due to offsets in samples, preparations, device manufacture, noise filtration, technician sample handling, and so forth. Further, recent advancements in amino acid synthesis have allowed the generation of millions of peptide and protein sequences. 
     Thus, what is needed in the art is a method of analyzing the voluminous amounts of data that is being produced by such devices. 
     SUMMARY OF THE INVENTION 
     The present invention satisfies this need through a method of determining unique amino acid sequences between two groups of samples as analyzed by mass spectrometer, such as a quadrupole ion trap. MS data from multiple samples is received from an MS ion trap, the data being normalized and may include noise reduction. MS data (dta) is provided in an ASCII format. The data may include noise reduction of the MS as a filter setting forth data files (dta). The Raw data is placed into an Inner-sample pattern match performed by merging same ions to form a first group (patient) and a second group (control). Each group is then pattern matched pairwise by merging ions with the same pattern between two samples taken from each group, to form an inter-group pattern match. The pattern provides a first group of unique ions, a second group of unique ions, and a third group having common ions between the first and second group. This is used to create data directories for use with the data files. The software bundled with the MS unit, such as SEQUEST, can then be used for word matching. In this manner an Inter-group comparison is performed for protein matching (multiple ions) and peptide matching (single ions). 
     In operation, samples are first prepared wherein daughter ions ejected having a particular Dalton range for each DTA sample are merged and the DTA&#39;s represented by a single charge state. Parent ions are also merged by scan numbers and the differences of each parent ion mass drop thereby creating a working file for each of the samples which contain data of all DTA&#39;s. 
     Each working file is queried to obtain the parent mass (Mqi) and charge state (Cqi) for each parent ion (Qi) and a target working file is then queried to obtain target DTA list (T 1 -Tm) having a parent mass (Mt 1 -Mtm) drop in the range of Mqi±1.5 Dalton and where their charge states (Ct 1 -Ctm) is the same as Cqi. 
     The parent ion is compared with each of the daughter patterns, the daughter ions that match are removed and the use of a scoring system is used to record each match. The scoring system provides a cumulative score; Q_ratio (matched/Total_Query ratio); T_ratio (matched/Total_Target ratio) and t_score (3*((Q ratio+T_ratio)/2)*Rs) where Rs is the acceptable ratio (in %) of matched over total. 
     The common ions (sample 12 _common) having matched candidates between Qi and Tj are recorded, and unmatched candidates are separated into separate files (sample 1 _unique) and (sample 2  unique), the samples are compared to obtain pair matches of parent mass and charge state. The matches are then clustered and the daughter ion patterns are compared within each of the clusters, calculating the ratio of the matched sample #/Total sample # (Rc) wherein the ion will be included in a final group common list if Rc≧Rm. 
     A single file can then be constructed naming Groupx_whole (uniques+commons) for each file which contains DTA names(s), parent mass, charge state, and all daughter ions. DTA directories are then constructed based on the above files to recover all the DTAs with different charge states, recover original data format for each DTA, recover original file name for each DTA, and create supporting files for a SEQUEST or similar type search for further filtering. Groups can then be summarized on protein names to generate files: a. Group 1 _unique with_name, b. Group 2  unique_with_name, c. Group 1 _unique_without name, d. Group 2 _unique_without_name; wherein Group_unique without_name list is used for optional databases searches, and Group_unique_with_name list is used directly for further analysis such as data mining. 
     An objective of the invention is to provide a method of finding unique protein species between groups of samples provided through mass spectrometry. 
     Another objective of the invention is to disclose an algorithm for subtracting of common ions between ion samples providing a base for optional database searches for use in further analysis. 
     Another objective of the invention is to teach the use of ion subtraction between two samples by taking advantage of daughter ion pattern in MS 2  spectrum and scan numbers for parent ions. 
     Still another objective of the invention is to teach the use of ion subtraction with multiple filters in sequence. 
     Still another objective of the invention is to disclose the use of a scoring system and/or matching ratio for locating of common ions. 
     Another objective of the invention is to disclose the use of a word-matcher. 
     Other objectives and advantages of this invention will become apparent from the following description taken in conjunction with the accompanying drawings wherein are set forth, by way of illustration and example, certain embodiments of this invention. The drawings constitute a part of this specification and include exemplary embodiments of the present invention and illustrate various objects and features thereof. 
    
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         FIG. 1A  is a flow chart for the pattern matcher program of the instant invention; 
         FIG. 1B  is a continuation of the flow chart of  FIG. 1A ; 
         FIG. 2  is a flow chart of inner sample pattern matching; 
         FIG. 3  is a flow chart of inner group pattern matching; 
         FIG. 4  is a flow chart of inter group pattern matching; 
         FIG. 5  is a chart comparing scan numbers versus sequence of the common ions to be MS/MS. 
     
    
    
     DETAILED DESCRIPTION 
     Referring now in general to the Figures, input data (DTA&#39;s) are obtained from a mass spectrometer, such as a Finnigan, or the like MS, that is converted into an ASCII format. The DTA can be obtained from software bundled with the particular MS, such as Sequest, Qstar, Sonar, and so forth. The MS or software may include noise reduction provisions particular to the MS device for normalization of the DTA. The DTA collected include MS spectrum data for a specific ion, or peptide, and in particular the Parent Mass and Charge State, and the daughter ion mass and intensity pairs to form a working file. 
     For data preparation, daughter peak merging is performed by merging all daughter ions ejected within the range of ±0.3 Dalton. It is well known that the machine reading of the mass of an ion, both parent ions and daughter ions, produces variations. Variations claimed by manufacturers is ±0.3 dalton, however, variations of ±1.5 daltons has been observed in parent masses and variations of ±1 daltons has been observed in daughter ion masses. Thus, daughter ions which drop in the same range are considered the same daughter ions as it is very rare for two different daughter ions from b and y series to have masses so close. A list is created of all daughter ions that are represented by only one charge state wherein the different charge state does not change the daughter composition, and the scan number is the same. 
     The parent ions in each sample are then merged with adjacent scan number and the differences of the masses of the parent ion drop within the range of about 0.5-1.0 Dalton. The above principles are used to create a working file for each sample which contains detailed data of all DTA&#39;s, including DTA file name and scan number, parent mass and charge state, and a list of the daughter ion masses and intensity pairs. 
     Daughter ion subtraction takes place between a first query sample (Sample 1 ) compared to a second sample (Target). The working file, previously generated as defined above, is read for each of the parent ions in Query DTA (qi) to obtain parent mass (Mqi) and charge state (Cqi). The Target working file is queried with both Mqi and Cqi, to obtain the target DTA list (T 1 -Tm) with their parent masses (Mt 1 -Mtm) drop in the range of Mqi±1.5 Dalton, and their charge states (Ct 1 -Ctm) is the same to Cqi:
 
 Mtj=Mqi ±1.5 Dalton
 
 Ctj=Cqi  Where 1 ≦j≦m 
 
     Qi is compared with each of the Tj in T 1 -Tm list of daughter patterns. If no match is found, the query MS 2  is placed in a unique list. If a match is found, the match is filtered by comparison by a scoring system. 
     In the scoring system, each daughter ion (Qik)in Qi is compared to the whole list of daughters (Tj 1 -Tjn) in Tj. It is considered as a match, and Qik and Tjg will be removed from Qi and Tj, respectively, if
 
| |Qik-Tjg| |≦±1 Dalton, where 1 ≦g≦n 
 
     The match is awarded a score based on the following scheme (for partition=4):
         Score=5, if |Qik-Tjg|≦±0.25 Dalton;   Score=4, if |Qik-Tjg|≦±0.50 Dalton;   Score=2, if |Qik-Tjg|≦±0.75 Dalton;   Score=1, if |Qik-Tjg|≦±1.00 Dalton.       

     The comparison will provide the following values:
         a. S: cumulative score;   b. Q_ratio: matched/Total_Query ratio;   c. T_ratio: matched/Total_Target ratio;   d. t_score=3*((Q_ratio+T_ratio)/2)*Rs
           Rs: acceptable ratio (in %) of matched over total.   
               

     The comparison will be considered as a pattern match candidate, to be included into the list of matched candidates QT, if the following conditions are met:
         a. S&gt;t_score;   b. Total_Target&gt;Td and Total_Query&gt;Td;   c. 1≦Total_Query or Total_Query/Total_Target≦2.       

     If the QT list contains a single candidate, then take this candidate as the match and remove Qi and Ti from Query and Target list, respectively. 
     If the QT list contains more than one match candidates, the list is filtered to find a match by the following method:
         a. Calculate DQT:
           DQT=Medium (Scan_Qi)−Medium (Scan_Tj)   Note: scan number for a DTA can be a range.   
           b. Compare DQT to Standard DQT between the samples by trend:
           DQT=Scan_Q1−Scan_T1 or   DQT=Scan_Qi−Scan_Tj.   
               

     Where Scan_Qi and Scan_Tj are considered the best match between two samples. If DQT&gt;1 and Dqt&lt;1, then it is likely that Tj belongs to a different peptide and Tj is to be removed from QT list.
         c. Compare Dqt to FQT between the sample by distance:
           FQT=k*((Scan_Qi+Scan_Tj)/2)
 
Where k is the slope of a linear function Scan_QT=f(dqt)=k*dqt+b. The function of f(dqt) is proximated to linear correlation.
   
           d. Choose the Tj with the highest score Sj from remaining QT list to be the final match.       

     It should be noted that each of the DTA&#39;s have a specific scan number, which is the indication when an ion has been out of a column and analyzed by MS/MS. The sequence of a scan number for a sample is fairly stable. However, the scan numbers shift between sample runs. If there is the same ion in both samples to be compared, scan number of this ion in one of the samples will differ from another sample. The distance of this same ion in two samples is the function of the scan numbers, in the range of 20 (scan number 600)-400 (scan number 4000).  FIG. 5  depicts a typical curve drawn with the data from extracted common ions from two samples as discussed in this disclosure. 
     After all the Query DTAs have been used for comparisons, a consensus file is generated which contains the common ions between the two samples. Files are generated for ions unique to each sample:
         a. sample 12 _common (each matched ions&#39; DTA names, parent masses, charge states, pattern daughters).   b. sample 1 _unique;   c. sample 2 _unique.       

     5. Within each group, pairing samples in all possible ways to the pairwise comparison as above. The total number of the comparison is given out by: 
         ∑     I   =   n     2     ⁢     (     I   -   1     )         
         Where n is the number of samples with the group.       

     The sample 12 _common files are merged and, and hash all the entries (pair matches) with parent mass+charge state. Cluster all the entries on their parent mass+charge state, with variation of ±1.5 Dalton. So, the members of each clusters are considered the candidates for extended matches outside of the original pairwise matches. 
     Daughter ion pattern comparison is performed within each of the clusters on the pattern daughter ions. The ratio Rc(the matched sample #/Total sample #) is then calculated. The ion will be included in the final group_common list if:
 
 Rc≧Rm 
 
     If there is any mismatch within the cluster, then create another cluster to include them. Rc is calculated for each of these sub-clusters, and evaluated as follows:
 
 Rc≧Rm 
 
     One file is generated for each group:
         Groupx_whole (uniques+commons), which should contain:   DTA names(s), parent mass, charge state, all daughter ions.       

     The scoring system is repeated to compare the 2 final group files. The final files generated are:
         a. Group 1 _unique;   b. Group 2 _unique;   c. Group 12 _common.       

     The 3 DTA directories are reconstructed based on the above files. The DTA reconstruction should be:
         a. Recover all the DTAs with different charge states;   b. For each DTA, recover original data format;   c. For each DTA, recover original file name;   d. Create supporting files for software (e.g. SEQUEST, QStar) search.
 
The software bundled with the MS can be further used to compare the peptide sequences among those single ions. This allows the uncharacterized ions to be reviewed in view of their correlation among those ions tagged with the same peptide sequence. The sequences predicated by the bundled program, such as SEQUEST, is not of interest in this step, rather, the correlations are of interest. Further, the sequences predicated along with the protein name associated with the sequence provide research assistance.
       

     The SEQUEST Search allows further filtering: However, instead of having M*N number of SEQUEST searches, there are only 3 summary results:
         a. One for common ones, which is optional, for reference;   b. One for Group 1 ;   c. One for Group 2 .       

     2. Subtracting Group 1  and Group 2  summary files on protein names generates files:
         a. Group 1 _unique_with_name;   b. Group 2  unique_with_name;   c. Group 1 _unique_without_name;   d. Group 2 _unique_without_name.       

     Group_unique_without_name list should be used for optional databases searches, e.g., with 6 reading frame databases. 
     Group_unique_with_name list can be used directly for further analysis, including data mining. 
     EXAMPLE 1 
     Unique Proteins in Control Group. 
     
       
         
               
             
               
             
               
             
               
             
               
             
               
             
           
               
                   
               
             
             
               
                 Proteins sharing keywords with the subtracted. 
               
             
          
           
               
                 (Format: Protein name and IDs: Keywords) 
               
               
                 gi|2258128|emb|CAA69 complement 9 [ homo sapiens ] [mass = 60398]: 
               
               
                 complement 
               
               
                 C05ADEE16G02.0791.0793.2.dta 
               
               
                 C05ADEE16G02.2549.2549.3.dta 
               
               
                 C05ADEE16G02.2537.2539.2.dta 
               
               
                 0145ADEE17G02.2909.2909.2.dta 
               
               
                 C05ADEE16G02.1803.1805.2.dta 
               
               
                 C05ADEE16G02.1666.1676.2.dta 
               
               
                 0145ADEE17G02.2064.2074.2.dta 
               
               
                 0154ADEE16G02.2064.2064.2.dta 
               
               
                 C05ADEE16G02.2062.2064.2.dta 
               
               
                 0154ADEE16G02.2235.2239.2.dta 
               
             
          
           
               
                 The Rest of the Unique Proteins. 
               
             
          
           
               
                 gi|223002|prf||04011: fibrin beta [ homo sapiens ] [mass = 50763] 
               
               
                 C05ADEE16G02.2294.2324.2.dta 
               
               
                 C05ADEE16G02.1281.1283.2.dta 
               
               
                 C07ADEE16G02.1281.1284.2.dta 
               
               
                 C07ADEE16G02.1581.1587.3.dta 
               
               
                 C07ADEE16G02.2310.2312.2.dta 
               
               
                 0154ADEE16G02.2672.2690.2.dta 
               
               
                 C05ADEE16G02.0811.0813.3.dta 
               
               
                 C07ADEE16G02.0811.0813.3.dta 
               
               
                 0145ADEE17G02.2665.2667.2.dta 
               
               
                 0145ADEE17G02.1962.1964.2.dta 
               
               
                 0145ADEE17G02.1217.1223.3.dta 
               
               
                 0145ADEE17G02.1219.1221.2.dta 
               
               
                 C07ADEE16G02.1219.1221.2.dta 
               
               
                 0154ADEE16G02.1206.1208.2.dta 
               
               
                 0145ADEE17G02.1203.1205.2.dta 
               
               
                 0154ADEE16G02.1721.1723.3.dta 
               
               
                 C07ADEE16G02.0797.0801.2.dta 
               
               
                 C05ADEE16G02.0807.0809.2.dta 
               
               
                 C07ADEE16G02.1343.1345.2.dta 
               
               
                 0145ADEE17G02.1012.1014.2.dta 
               
               
                 0154ADEE16G02.1959.1961.2.dta 
               
               
                 C05ADEE16G02.1959.1961.2.dta 
               
             
          
           
               
                 Summary list, refer above for IDs and DTA files. 
               
             
          
           
               
                 complement 9 [ homo sapiens ] [mass = 60398] 
               
               
                 fibrin beta [ homo sapiens ] [mass = 50763] 
               
               
                   
               
             
          
         
       
     
     EXAMPLE 2 
     Unique Single Ions in Control Group. 
     
       
         
               
             
               
               
               
             
           
               
                   
               
               
                 Unique Single Ions in Control Group, With Multiple Samples 
               
               
                 Matches For Peptide 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 2: 
                 ADSQAQLLLSTVVGVFTAPGLHLK gi|15079348 
               
               
                   
                   
                 C05ADEE16G02.2824.2832.3.dta 
               
               
                   
                   
                 C07ADEE16G02.2834.2834.2.dta 
               
               
                   
                 3: 
                 AEMEVQMMKKELDQK gi|17563838 
               
               
                   
                   
                 C05ADEE16G02.0916.0918.3.dta 
               
               
                   
                   
                 C07ADEE16G02.0918.0920.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1321.1323.3.dta 
               
               
                   
                 2: 
                 ALDRHQNSPAPR gi|20544433 
               
               
                   
                   
                 C07ADEE16G02.1363.1363.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1751.1753.2.dta 
               
               
                   
                 3: 
                 ALFATSTAGLLRCPR gi|21229522 
               
               
                   
                   
                 C07ADEE16G02.0643.0643.3.dta 
               
               
                   
                   
                 C05ADEE16G02.0647.0649.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1033.1035.3.dta 
               
               
                   
                 2: 
                 ANELIKLVGLEGK gi|16080327 
               
               
                   
                   
                 0145ADEE17G02.2463.2469.1.dta 
               
               
                   
                   
                 0154ADEE16G02.2468.2470.2.dta 
               
               
                   
                 2: 
                 APVAGLSGAAGGSTLYSFEAK gi|21244270 
               
               
                   
                   
                 C05ADEE16G02.2391.2391.3.dta 
               
               
                   
                   
                 0145ADEE17G02.2487.2487.2.dta 
               
               
                   
                 2: 
                 AQEGPDAEIIGGERSVFYLLMK gi|12644073 
               
               
                   
                   
                 C05ADEE16G02.1730.1732.3.dta 
               
               
                   
                   
                 0145ADEE17G02.2126.2128.3.dta 
               
               
                   
                 2: 
                 ASDSVQSALKNR gi|15238480 
               
               
                   
                   
                 C07ADEE16G02.1036.1038.3.dta 
               
               
                   
                   
                 C05ADEE16G02.1038.1038.3.dta 
               
               
                   
                 2: 
                 ASLTRTPPTASLTR gi|18594161 
               
               
                   
                   
                 C05ADEE16G02.1712.1714.2.dta 
               
               
                   
                   
                 C07ADEE16G02.1720.1722.2.dta 
               
               
                   
                 3: 
                 CLDSVLGEFAFPDEFVVE gi|7522626 
               
               
                   
                   
                 C05ADEE16G02.2296.2314.3.dta 
               
               
                   
                   
                 C07ADEE16G02.2328.2358.3.dta 
               
               
                   
                   
                 0154ADEE16G02.2698.2700.3.dta 
               
               
                   
                 2: 
                 DALTGHLRTHSVGKPHK gi|1911483 
               
               
                   
                   
                 C05ADEE16G02.1837.1841.3.dta 
               
               
                   
                   
                 0145ADEE17G02.2230.2234.3.dta 
               
               
                   
                 2: 
                 DAVKFGRMSK gi|1082764 
               
               
                   
                   
                 C05ADEE16G02.1503.1507.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1893.1895.2.dta 
               
               
                   
                   
                 C05ADEE16G02.1893.1895.2.dta 
               
               
                   
                 2: 
                 DEINSLIGQKNVLFFK gi|15896245 
               
               
                   
                   
                 C07ADEE16G02.1465.1465.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1849.1849.2.dta 
               
               
                   
                 2: 
                 DRVLMGPARTGGR gi|17554264 
               
               
                   
                   
                 C05ADEE16G02.1255.1265.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1651.1665.2.dta 
               
               
                   
                 2: 
                 DWLFSLKISEVK gi|20502693 
               
               
                   
                   
                 C07ADEE16G02.0787.0793.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1199.1199.2.dta 
               
               
                   
                 2: 
                 DYIFGNYIER gi|10092579 
               
               
                   
                   
                 C05ADEE16G02.1630.1632.2.dta 
               
               
                   
                   
                 C07ADEE16G02.1641.1643.2.dta 
               
               
                   
                 2: 
                 EGTIGDMAILGITESFQVKR gi|11493524 
               
               
                   
                   
                 C05ADEE16G02.2136.2188.3.dta 
               
               
                   
                   
                 0154ADEE16G02.2562.2564.3.dta 
               
               
                   
                 2: 
                 EHLVQATPENLQEAR gi|10092579 
               
               
                   
                   
                 C07ADEE16G02.0853.0857.3.dta 
               
               
                   
                   
                 C05ADEE16G02.0864.0864.3.dta 
               
               
                   
                 2: 
                 EKVLAYK gi|19913514 
               
               
                   
                   
                 C07ADEE16G02.1914.1914.1.dta 
               
               
                   
                   
                 0145ADEE17G02.2294.2294.1.dta 
               
               
                   
                 2: 
                 ELINYNAFARDFK gi|8250394 
               
               
                   
                   
                 C05ADEE16G02.1877.1887.2.dta 
               
               
                   
                   
                 0145ADEE17G02.2274.2274.2.dta 
               
               
                   
                 2: 
                 EMEVYRVTESLQR gi|14249254 
               
               
                   
                   
                 0154ADEE16G02.1979.1981.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1984.1988.2.dta 
               
               
                   
                 2: 
                 EPNNNIGRKH gi|17440552 
               
               
                   
                   
                 C05ADEE16G02.1235.1245.2.dta 
               
               
                   
                   
                 C07ADEE16G02.1239.1239.2.dta 
               
               
                   
                 2: 
                 FSAEALRCHPR gi|29389 
               
               
                   
                   
                 C05ADEE16G02.2003.2005.2.dta 
               
               
                   
                   
                 C07ADEE16G02.2014.2016.2.dta 
               
               
                   
                 3: 
                 GALAQAQEVSLGADVGRNPR gi|17474963 
               
               
                   
                   
                 0145ADEE17G02.2380.2380.2.dta 
               
               
                   
                   
                 C05ADEE16G02.2503.2507.3.dta 
               
               
                   
                   
                 C07ADEE16G02.2523.2525.3.dta 
               
               
                   
                 2: 
                 GDGTDGVGSMPCGR gi|18580732 
               
               
                   
                   
                 0154ADEE16G02.1528.1528.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1529.1531.2.dta 
               
               
                   
                   
                 C05ADEE16G02.1529.1531.2.dta 
               
               
                   
                 2: 
                 GEVQAMLGQSTEELR gi|1480096 
               
               
                   
                   
                 C05ADEE16G02.1362.1384.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1739.1739.2.dta 
               
               
                   
                 3: 
                 GHPFLQEECKAPAR gi|20550933 
               
               
                   
                   
                 C05ADEE16G02.1024.1026.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1030.1030.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1030.1030.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1430.1435.3.dta 
               
               
                   
                 2: 
                 GLLDQGIPSK gi|18375646 
               
               
                   
                   
                 C07ADEE16G02.1157.1157.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1552.1552.3.dta 
               
               
                   
                 2: 
                 GLRKMLDNFDCFGDK gi|20380096 
               
               
                   
                   
                 0145ADEE17G02.1241.1241.3.dta 
               
               
                   
                   
                 C05ADEE16G02.1241.1241.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1241.1241.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1639.1639.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1639.1639.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1639.1639.3.dta 
               
               
                   
                 2: 
                 GLVLVVGSTGSGKSTSLASMIDHR gi|14090413 
               
               
                   
                   
                 C07ADEE16G02.1175.1181.3.dta 
               
               
                   
                   
                 C05ADEE16G02.1186.1190.3.dta 
               
               
                   
                 2: 
                 GVAVLHIGGGSEVEVNEK gi|17864606 
               
               
                   
                   
                 0154ADEE16G02.1172.1174.3.dta 
               
               
                   
                   
                 C05ADEE16G02.1172.1174.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1173.1175.3.dta 
               
               
                   
                 2: 
                 GWQVYGLALDSDTAPTLR gi|21243805 
               
               
                   
                   
                 C05ADEE16G02.0807.0809.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1206.1208.3.dta 
               
               
                   
                 2: 
                 GWRPYEIHSVTPIDEILNMFK gi|8923754 
               
               
                   
                   
                 C05ADEE16G02.1616.1618.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1635.1637.3.dta 
               
               
                   
                 2: 
                 HAQEELPPPPPQKKR gi|118685 
               
               
                   
                   
                 C07ADEE16G02.0839.0841.3.dta 
               
               
                   
                   
                 C05ADEE16G02.0848.0848.3.dta 
               
               
                   
                 2: 
                 HLALGCFGLNR gi|16805179 
               
               
                   
                   
                 C07ADEE16G02.0877.0879.2.dta 
               
               
                   
                   
                 C05ADEE16G02.0884.0886.2.dta 
               
               
                   
                 2: 
                 HTWSLLKEMAK gi|11493656 
               
               
                   
                   
                 C05ADEE16G02.2300.2306.1.dta 
               
               
                   
                   
                 0154ADEE16G02.2698.2700.2.dta 
               
               
                   
                 2: 
                 IARNIYIKVGEDQVFPPK gi|20535619 
               
               
                   
                   
                 C05ADEE16G02.2051.2057.3.dta 
               
               
                   
                   
                 C07ADEE16G02.2062.2074.3.dta 
               
               
                   
                 2: 
                 ILAITFTKK gi|12045098 
               
               
                   
                   
                 0154ADEE16G02.2259.2265.2.dta 
               
               
                   
                   
                 C05ADEE16G02.2328.2328.1.dta 
               
               
                   
                 2: 
                 KEGDGKGK gi|12843573 
               
               
                   
                   
                 C05ADEE16G02.0920.0922.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1327.1329.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1327.1329.2.dta 
               
               
                   
                 4: 
                 LAALSTATTVHHQSQHAVTTCYGR gi|20891466 
               
               
                   
                   
                 C05ADEE16G02.2401.2423.2.dta 
               
               
                   
                   
                 C07ADEE16G02.2423.2439.3.dta 
               
               
                   
                   
                 0145ADEE17G02.2761.2763.3.dta 
               
               
                   
                   
                 0145ADEE17G02.2783.2783.2.dta 
               
               
                   
                 2: 
                 LAGRILGMGDVASLAEK gi|3024639 
               
               
                   
                   
                 C05ADEE16G02.2393.2397.2.dta 
               
               
                   
                   
                 0154ADEE16G02.2778.2784.2.dta 
               
               
                   
                 2: 
                 LAPPPVAELR gi|3915680 
               
               
                   
                   
                 C07ADEE16G02.0970.0976.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1374.1376.2.dta 
               
               
                   
                 2: 
                 LLWLVLQPFFYSLR gi|18583655 
               
               
                   
                   
                 C05ADEE16G02.2017.2019.3.dta 
               
               
                   
                   
                 0145ADEE17G02.2400.2404.3.dta 
               
               
                   
                 2: 
                 LNNGEITQHR gi|29565 
               
               
                   
                   
                 C07ADEE16G02.0580.0583.2.dta 
               
               
                   
                   
                 C05ADEE16G02.0584.0586.2.dta 
               
               
                   
                 2: 
                 LRGEAAAGAAGMKR gi|17939383 
               
               
                   
                   
                 C07ADEE16G02.0680.0680.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1081.1081.2.dta 
               
               
                   
                 2: 
                 LSSLTPCPN gi|8922394 
               
               
                   
                   
                 0154ADEE16G02.1107.1109.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1108.1108.2.dta 
               
               
                   
                 3: 
                 LSWLEGGVR gi|15643398 
               
               
                   
                   
                 C07ADEE16G02.0783.0783.2.dta 
               
               
                   
                   
                 C05ADEE16G02.0784.0789.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1186.1196.2.dta 
               
               
                   
                 2: 
                 MGGAAWDGEKLSVGSGK gi|20473307 
               
               
                   
                   
                 C05ADEE16G02.1047.1066.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1452.1456.3.dta 
               
               
                   
                 2: 
                 MGPTTSPVPR gi|20541193 
               
               
                   
                   
                 0145ADEE17G02.1261.1263.2.dta 
               
               
                   
                   
                 C05ADEE16G02.1261.1263.2.dta 
               
               
                   
                   
                 C07ADEE16G02.1265.1267.2.dta 
               
               
                   
                 2: 
                 MLAPSGSSGVWR gi|6912282 
               
               
                   
                   
                 C05ADEE16G02.0518.0520.2.dta 
               
               
                   
                   
                 0154ADEE16G02.0898.0900.2.dta 
               
               
                   
                 2: 
                 MQLVDHRGGGGGGGKGGR gi|13787204 
               
               
                   
                   
                 0145ADEE17G02.1024.1024.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1024.1024.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1024.1024.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1421.1421.3.dta 
               
               
                   
                 2: 
                 NGWLNLEFENR gi|20541427 
               
               
                   
                   
                 C07ADEE16G02.2062.2074.2.dta 
               
               
                   
                   
                 0154ADEE16G02.2434.2436.2.dta 
               
               
                   
                 2: 
                 NPIVLSMYFTKQLYR gi|14733277 
               
               
                   
                   
                 C05ADEE16G02.1146.1146.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1537.1537.2.dta 
               
               
                   
                 2: 
                 NYADQISRLEERESEMK gi|10432429 
               
               
                   
                   
                 C05ADEE16G02.1446.1450.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1455.1457.3.dta 
               
               
                   
                 2: 
                 PERVLIIGGGDGGVVR gi|10945607 
               
               
                   
                   
                 C05ADEE16G02.1078.1078.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1092.1092.3.dta 
               
               
                   
                 2: 
                 PIENRILILPSVTR gi|1071965 
               
               
                   
                   
                 C07ADEE16G02.0735.0737.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1137.1143.3.dta 
               
               
                   
                 2: 
                 PSIPQQEHTAVAKHGPTEKTLR gi|20553669 
               
               
                   
                   
                 C05ADEE16G02.0774.0776.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1176.1182.3.dta 
               
               
                   
                 3: 
                 QEHDLVFAVR gi|21243872 
               
               
                   
                   
                 C05ADEE16G02.2409.2409.1.dta 
               
               
                   
                   
                 C07ADEE16G02.2419.2421.2.dta 
               
               
                   
                   
                 0145ADEE17G02.2755.2781.1.dta 
               
               
                   
                 2: 
                 RILNRLQPR gi|17456789 
               
               
                   
                   
                 C07ADEE16G02.0605.0605.3.dta 
               
               
                   
                   
                 0154ADEE16G02.0996.0998.3.dta 
               
               
                   
                 3: 
                 RIMVITVSLIMLR gi|14042829 
               
               
                   
                   
                 C07ADEE16G02.1042.1044.3.dta 
               
               
                   
                   
                 C05ADEE16G02.1043.1045.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1446.1474.3.dta 
               
               
                   
                 2: 
                 RLGSVQR gi|20551188 
               
               
                   
                   
                 C07ADEE16G02.0597.0599.2.dta 
               
               
                   
                   
                 C05ADEE16G02.0598.0600.2.dta 
               
               
                   
                 2: 
                 RPAPSGGASGDLRAFGTR gi|1082358 
               
               
                   
                   
                 C07ADEE16G02.0580.0583.3.dta 
               
               
                   
                   
                 C05ADEE16G02.0584.0586.3.dta 
               
               
                   
                 2: 
                 RQDELIVLNVSGR gi|14739334 
               
               
                   
                   
                 C07ADEE16G02.2998.3006.2.dta 
               
               
                   
                   
                 C05ADEE16G02.3002.3002.2.dta 
               
               
                   
                 3: 
                 RQQMQDFFLAHK gi|10952524 
               
               
                   
                   
                 C07ADEE16G02.0623.0627.3.dta 
               
               
                   
                   
                 C05ADEE16G02.0633.0637.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1032.1034.3.dta 
               
               
                   
                 2: 
                 RSPTGSTTSRASTVSTK gi|7513102 
               
               
                   
                   
                 C05ADEE16G02.1110.1144.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1522.1546.3.dta 
               
               
                   
                 2: 
                 RYNLSLLQTLMFNSARMSFR gi|18978275 
               
               
                   
                   
                 C07ADEE16G02.1968.1972.3.dta 
               
               
                   
                   
                 0154ADEE16G02.2341.2351.3.dta 
               
               
                   
                 2: 
                 SAFKAVLHQPLKVIR gi|7662659 
               
               
                   
                   
                 C05ADEE16G02.1503.1507.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1511.1513.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1511.1513.3.dta 
               
               
                   
                 2: 
                 SEAEDASLLSFMQGYMK gi|224917 
               
               
                   
                   
                 C07ADEE16G02.2192.2192.3.dta 
               
               
                   
                   
                 0154ADEE16G02.2546.2552.2.dta 
               
               
                   
                 2: 
                 SGSYSYLEERK gi|21360806 
               
               
                   
                   
                 C07ADEE16G02.0936.0938.2.dta 
               
               
                   
                   
                 C05ADEE16G02.0941.0943.2.dta 
               
               
                   
                 3: 
                 SIVHPSYNSNTLNNDIMLIK gi|15988427 
               
               
                   
                   
                 C07ADEE16G02.1517.1519.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1673.1673.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1908.1910.2.dta 
               
               
                   
                 2: 
                 SNDMNWAHRASR gi|106716 
               
               
                   
                   
                 C05ADEE16G02.2136.2188.2.dta 
               
               
                   
                   
                 0145ADEE17G02.2567.2583.2.dta 
               
               
                   
                 4: 
                 SPLGEEMR gi|2392352 
               
               
                   
                   
                 C05ADEE16G02.0821.0823.2.dta 
               
               
                   
                   
                 C07ADEE16G02.0821.0823.2.dta 
               
               
                   
                   
                 C05ADEE16G02.0832.0832.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1227.1229.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1228.1230.2.dta 
               
               
                   
                   
                 C05ADEE16G02.1228.1230.2.dta 
               
               
                   
                 2: 
                 SQNDFDLVPWLQIATQLISK gi|1154664 
               
               
                   
                   
                 C05ADEE16G02.1068.1070.3.dta 
               
               
                   
                   
                 C07ADEE16G02.1070.1072.3.dta 
               
               
                   
                 2: 
                 SSSVGCLAEASAR gi|10047175 
               
               
                   
                   
                 0154ADEE16G02.1222.1224.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1225.1225.2.dta 
               
               
                   
                   
                 C07ADEE16G02.1225.1225.2.dta 
               
               
                   
                 3: 
                 TATSEYQTFFNPR gi|1335344 
               
               
                   
                   
                 0145ADEE17G02.1406.1408.2.dta 
               
               
                   
                   
                 C05ADEE16G02.1406.1408.2.dta 
               
               
                   
                   
                 C07ADEE16G02.1415.1421.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1812.1812.2.dta 
               
               
                   
                 2: 
                 TMGYSQEIVEK gi|20562022 
               
               
                   
                   
                 C05ADEE16G02.2009.2009.1.dta 
               
               
                   
                   
                 0145ADEE17G02.2388.2390.1.dta 
               
               
                   
                 2: 
                 TSPSNSKNICMPTTDNNNNSSSSTDDTK gi|256818 
               
               
                   
                   
                 C05ADEE16G02.0973.0979.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1381.1381.3.dta 
               
               
                   
                 2: 
                 TVFPGAVPVLPASPPPK gi|10864047 
               
               
                   
                   
                 C07ADEE16G02.0849.0855.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1356.1358.2.dta 
               
               
                   
                   
                 0154ADEE16G02.1356.1358.2.dta 
               
               
                   
                   
                 C05ADEE16G02.1356.1358.2.dta 
               
               
                   
                 2: 
                 VESLFLDERMSLTQRIGK gi|17457181 
               
               
                   
                   
                 C05ADEE16G02.0842.0842.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1238.1240.3.dta 
               
               
                   
                 2: 
                 VGFYESDVMGR gi|17455493 
               
               
                   
                   
                 C07ADEE16G02.1290.1290.2.dta 
               
               
                   
                   
                 0145ADEE17G02.1683.1683.2.dta 
               
               
                   
                   
                 C07ADEE16G02.1683.1683.2.dta 
               
               
                   
                 2: 
                 VIEMVTNLVNINTNK gi|20543383 
               
               
                   
                   
                 C05ADEE16G02.1320.1320.2.dta 
               
               
                   
                   
                 C07ADEE16G02.1331.1331.2.dta 
               
               
                   
                 2: 
                 VIERYMPRAFGR gi|11499504 
               
               
                   
                   
                 C05ADEE16G02.1738.1738.2.dta 
               
               
                   
                   
                 0154ADEE16G02.2139.2145.2.dta 
               
               
                   
                 2: 
                 VLNPPATFLYR gi|21232394 
               
               
                   
                   
                 C07ADEE16G02.1265.1267.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1657.1659.3.dta 
               
               
                   
                 3: 
                 VLLPQVGKLFKK gi|6322590 
               
               
                   
                   
                 C07ADEE16G02.0747.0751.3.dta 
               
               
                   
                   
                 C05ADEE16G02.0753.0753.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1153.1155.3.dta 
               
               
                   
                 3: 
                 VTVAGIEQRFCQQCSR gi|15227896 
               
               
                   
                   
                 C05ADEE16G02.0960.0964.3.dta 
               
               
                   
                   
                 C07ADEE16G02.0966.0968.3.dta 
               
               
                   
                   
                 0145ADEE17G02.1366.1368.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1366.1368.3.dta 
               
               
                   
                 2: 
                 VVIAPAGITHDPR gi|21233290 
               
               
                   
                   
                 C07ADEE16G02.2328.2358.2.dta 
               
               
                   
                   
                 0145ADEE17G02.2671.2673.2.dta 
               
               
                   
                 3: 
                 WEQLIAFK gi|6759114 
               
               
                   
                   
                 C05ADEE16G02.1871.1873.1.dta 
               
               
                   
                   
                 C07ADEE16G02.1894.1896.1.dta 
               
               
                   
                   
                 0145ADEE17G02.2260.2264.1.dta 
               
               
                   
                 2: 
                 WQQGHQLAIMKGFQK gi|12834350 
               
               
                   
                   
                 0154ADEE16G02.1278.1280.3.dta 
               
               
                   
                   
                 C05ADEE16G02.1484.1486.3.dta 
               
               
                   
                 2: 
                 YEDKTCPLKK gi|16080632 
               
               
                   
                   
                 C05ADEE16G02.0928.0928.1.dta 
               
               
                   
                   
                 0145ADEE17G02.1340.1340.1.dta 
               
               
                   
                 2: 
                 YLKAIR gi|21242998 
               
               
                   
                   
                 C05ADEE16G02.0878.0880.1.dta 
               
               
                   
                   
                 0145ADEE17G02.1279.1279.1.dta 
               
               
                   
                 2: 
                 YSKLKEK gi|13431562 
               
               
                   
                   
                 C05ADEE16G02.0462.0466.2.dta 
               
               
                   
                   
                 0154ADEE16G02.0560.0562.2.dta 
               
               
                   
                 2: 
                 YTWHEATLGK gi|11065769 
               
               
                   
                   
                 C07ADEE16G02.0637.0637.3.dta 
               
               
                   
                   
                 0154ADEE16G02.1010.1010.3.dta 
               
               
                   
                 3: 
                 Apolipoprotein e [ homo sapiens ] 
               
               
                   
                   
                 C05ADEE16G02.2062.2064.3.dta 
               
               
                   
                   
                 0145ADEE17G02.2066.2068.2.dta 
               
               
                   
                   
                 C05ADEE16G02.2066.2068.2.dta 
               
               
                   
                   
                 C07ADEE16G02.2082.2082.2.dta 
               
               
                   
                 1: 
                 Hypothetical protein xp_106741 [ homo sapiens ] 
               
               
                   
                   
                 C05ADEE16G02.0938.0951.3.dta 
               
               
                   
                 1: 
                 Similar to glyceraldehyde 3-phosphate dehydrogenase, liver 
               
               
                   
                   
                 [ homo sapiens ] 
               
               
                   
                   
                 0145ADEE17G02.2757.2759.3.dta 
               
               
                   
                   
               
             
          
         
       
     
     EXAMPLE 3 
     Proteins and Single Ions Subtracted by Either Pattern Matching or Word Matching 
     
       
         
               
             
               
             
               
             
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
               
             
               
               
             
           
               
                   
               
             
             
               
                 Protein subtracted based on both pattern-matching and IDs. 
               
             
          
           
               
                 gi|11761629|ref|NP_0: a-alpha fibrinogen [ homo sapiens ] 
               
               
                 gi|178345|gb|AAA9879: alloalbumin venezia [ homo sapiens ] 
               
               
                 [mass = 69227] 
               
               
                 gi|4502149|ref|NP_00: apolipoprotein a-ii precursor [ homo   
               
               
                   sapiens ] 
               
               
                 gi|178730|gb|AAB0048: apolipoprotein b-100 precursor [ homo   
               
               
                   sapiens ] [mass = 515558] 
               
               
                 gi|178855|gb|AAA5176: apolipoprotein j precursor [ homo sapiens ] 
               
               
                 [mass = 48803] 
               
               
                 gi|1314244|gb|AAA997: complement c4b precursor [ homo sapiens ] 
               
               
                 [mass = 188405] 
               
               
                 gi|20545719|ref|XP_0: complement component 3 precursor [ homo   
               
               
                   sapiens ] [mass = 187148] 
               
               
                 gi|14577919|ref|NP_0: complement component 4a preproprotein; 
               
               
                 acidic c4; rodgers form of c4; complement component 4s [ homo   
               
               
                   sapiens ] [mass = 192336] 
               
               
                 gi|1070458|pir||KUHU: ferroxidase (ec 1.16.3.1) precursor - 
               
               
                 human [mass = 122652] 
               
               
                 gi|223918|prf||10043: fibrinogen alphaa [ homo sapiens ] 
               
               
                 [mass = 49397] 
               
               
                 gi|20178280|sp|P0267: fibrinogen gamma chain precursor (pro2061) 
               
               
                 gi|11761631|ref|NP_0: fibrinogen, beta chain preproprotein; 
               
               
                 fibrinogen, b beta polypeptide 
               
               
                 gi|16933542|ref|NP_0: fibronectin 1, isoform 1 preproprotein; 
               
               
                 cold-insoluble globulin 
               
               
                 gi|1212947|emb|CAA25: haptoglobin [ homo sapiens ] [mass = 38452] 
               
               
                 gi|11435220|ref|XP_0: inter-alpha (globulin) inhibitor, h1 
               
               
                 polypeptide [ homo sapiens ] 
               
               
                 gi|125000|sp|P19823|: inter-alpha-trypsin inhibitor heavy chain 
               
               
                 h2 precursor (iti heavy chain h2) hyaluronan-associated protein) 
               
               
                 (shap) 
               
               
                 gi|229479|prf||74052: lipoprotein gln i [ homo sapiens ] 
               
               
                 gi|178775|gb|AAA5174: proapolipoprotein [ homo sapiens ] 
               
               
                 [mass = 28962] 
               
               
                 gi|20551100|ref|XP_0: similar to dj34f7.4 (complement component 
               
               
                 4a) [ homo sapiens ] 
               
             
          
           
               
                 Protein subtracted based on names. 
               
             
          
           
               
                 No Match with the same name, but different IDs 
               
             
          
           
               
                 Common Single Ions Between Patient and Control Groups 
               
             
          
           
               
                 AKRELSQGISK: 
                 gi|20471541 
               
               
                 patient: 
                 2 
               
               
                   
                 12ADEE15G02.0958.0960.2.dta 
               
               
                   
                 18ADEE16G02.0978.0980.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 C07ADEE16G02.0966.0968.2.dta 
               
               
                   
                 0145ADEE17G02.1366.1368.2.dta 
               
               
                   
                 0154ADEE16G02.1366.1368.2.dta 
               
               
                 APKPKAAK: 
                 gi|4885377 
               
               
                 patient: 
                 3 
               
               
                   
                 31ADEE16G02.0685.0689.2.dta 
               
               
                   
                 20ADEE16G02.0698.0700.2.dta 
               
               
                   
                 18ADEE16G02.0705.0707.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 C07ADEE16G02.0700.0702.2.dta 
               
               
                   
                 0145ADEE17G02.1100.1102.2.dta 
               
               
                 EALAKGK: 
                 gi|4885231 
               
               
                 patient: 
                 3 
               
               
                   
                 12ADEE15G02.0655.0655.2.dta 
               
               
                   
                 20ADEE16G02.0668.0670.2.dta 
               
               
                   
                 18ADEE16G02.0679.0681.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 C05ADEE16G02.0670.0674.2.dta 
               
               
                   
                 0154ADEE16G02.1071.1073.2.dta 
               
               
                 EHVDVIAK: 
                 gi|20544347 
               
               
                 patient: 
                 2 
               
               
                   
                 31ADEE16G02.1267.1271.1.dta 
               
               
                   
                 18ADEE16G02.1293.1293.1.dta 
               
               
                 control: 
                 3 
               
               
                   
                 C05ADEE16G02.1285.1287.1.dta 
               
               
                   
                 C07ADEE16G02.1286.1288.1.dta 
               
               
                   
                 0154ADEE16G02.1679.1681.1.dta 
               
               
                 FEDGVLDPDYPR: 
                 gi|139653 
               
               
                 patient: 
                 3 
               
               
                   
                 20ADEE16G02.1301.1303.2.dta 
               
               
                   
                 31ADEE16G02.1306.1312.2.dta 
               
               
                   
                 12ADEE15G02.1325.1327.2.dta 
               
               
                   
                 18ADEE16G02.1325.1327.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 C05ADEE16G02.1316.1318.2.dta 
               
               
                   
                 0145ADEE17G02.1725.1725.2.dta 
               
               
                 FEEHKNEKDMAK: 
                 gi|4894370 
               
               
                 patient: 
                 3 
               
               
                   
                 2ADEE15G02.1523.1552.2.dta 
               
               
                   
                 12ADEE15G02.1556.1558.2.dta 
               
               
                   
                 20ADEE16G02.1556.1558.2.dta 
               
               
                   
                 18ADEE16G02.1563.1563.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 0145ADEE17G02.1561.1563.2.dta 
               
               
                   
                 0154ADEE16G02.1561.1563.2.dta 
               
               
                   
                 C05ADEE16G02.1561.1563.2.dta 
               
               
                   
                 C07ADEE16G02.1571.1575.2.dta 
               
               
                 FSEMQNERR: 
                 gi|21361704 
               
               
                 patient: 
                 2 
               
               
                   
                 20ADEE16G02.2009.2015.2.dta 
               
               
                   
                 20ADEE16G02.2021.2023.2.dta 
               
               
                   
                 31ADEE16G02.2021.2023.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 C05ADEE16G02.2017.2019.2.dta 
               
               
                   
                 0145ADEE17G02.2400.2404.2.dta 
               
               
                 HTLNQIDEVK: 
                 gi|2521981 
               
               
                 patient: 
                 3 
               
               
                   
                 20ADEE16G02.0766.0768.2.dta 
               
               
                   
                 18ADEE16G02.0772.0774.2.dta 
               
               
                   
                 31ADEE16G02.0773.0773.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 0154ADEE16G02.1172.1174.2.dta 
               
               
                   
                 C05ADEE16G02.1172.1174.2.dta 
               
               
                   
                 0145ADEE17G02.1173.1175.2.dta 
               
               
                 LDDIVDSVLATGIQR: 
                 gi|11359042 
               
               
                 patient: 
                 2 
               
               
                   
                 12ADEE15G02.1735.1735.2.dta 
               
               
                   
                 18ADEE16G02.1735.1735.2.dta 
               
               
                   
                 31ADEE16G02.1740.1746.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 C05ADEE16G02.1730.1732.2.dta 
               
               
                   
                 C07ADEE16G02.1744.1744.2.dta 
               
               
                 PAERGLPAPK: 
                 gi|1076013 
               
               
                 patient: 
                 4 
               
               
                   
                 20ADEE16G02.1865.1869.2.dta 
               
               
                   
                 12ADEE15G02.1870.1874.2.dta 
               
               
                   
                 31ADEE16G02.1873.1877.2.dta 
               
               
                   
                 18ADEE16G02.1873.1879.2.dta 
               
               
                 control: 
                 3 
               
               
                   
                 C05ADEE16G02.1867.1869.2.dta 
               
               
                   
                 C07ADEE16G02.1886.1892.2.dta 
               
               
                   
                 0145ADEE17G02.2256.2258.2.dta 
               
               
                 QVVTFVVLLSLCLLK: 
                 gi|18572033 
               
               
                 patient: 
                 2 
               
               
                   
                 18ADEE16G02.1373.1377.3.dta 
               
               
                   
                 12ADEE15G02.1377.1381.3.dta 
               
               
                 control: 
                 3 
               
               
                   
                 C05ADEE16G02.1378.1386.3.dta 
               
               
                   
                 C07ADEE16G02.1379.1383.3.dta 
               
               
                   
                 0145ADEE17G02.1774.1786.3.dta 
               
               
                 TYLPAVDEK: 
                 gi|114022 
               
               
                 patient: 
                 2 
               
               
                   
                 31ADEE16G02.0957.0959.2.dta 
               
               
                   
                 18ADEE16G02.0982.0984.2.dta 
               
               
                 control: 
                 3 
               
               
                   
                 C07ADEE16G02.0972.0974.2.dta 
               
               
                   
                 C05ADEE16G02.0975.0977.2.dta 
               
               
                   
                 0154ADEE16G02.1380.1380.2.dta 
               
               
                   
                 C05ADEE16G02.1380.1380.2.dta 
               
               
                 YVDKAILGDEDDMFR: 
                 gi|11499013 
               
               
                 patient: 
                 2 
               
               
                   
                 18ADEE16G02.1781.1785.3.dta 
               
               
                   
                 12ADEE15G02.1786.1788.3.dta 
               
               
                 control: 
                 3 
               
               
                   
                 C05ADEE16G02.1789.1791.3.dta 
               
               
                   
                 C07ADEE16G02.1796.1798.3.dta 
               
               
                   
                 0145ADEE17G02.2180.2182.3.dta 
               
             
          
           
               
                 Alpha2-hs glycoprotein [ homo sapiens ] 
               
             
          
           
               
                 patient: 
                 4 
               
               
                   
                 18ADEE16G02.1261.1263.3.dta 
               
               
                   
                 31ADEE16G02.1261.1263.3.dta 
               
               
                   
                 31ADEE16G02.0764.0766.2.dta 
               
               
                   
                 18ADEE16G02.1235.1237.3.dta 
               
               
                   
                 31ADEE16G02.1235.1237.3.dta 
               
               
                   
                 12ADEE15G02.1252.1254.3.dta 
               
               
                 control: 
                 3 
               
               
                   
                 C05ADEE16G02.1255.1265.3.dta 
               
               
                   
                 0145ADEE17G02.1649.1651.3.dta 
               
               
                   
                 C07ADEE16G02.1261.1269.3.dta 
               
             
          
           
               
                 Angiotensinogen (serine (or cysteine) proteinase inhibitor, 
               
               
                 clade a 
               
             
          
           
               
                 patient: 
                 3 
               
               
                   
                 18ADEE16G02.1625.1625.2.dta 
               
               
                   
                 20ADEE16G02.1604.1606.2.dta 
               
               
                   
                 31ADEE16G02.2238.2242.2.dta 
               
               
                 control: 
                 6 
               
               
                   
                 C05ADEE16G02.2234.2236.2.dta 
               
               
                   
                 C07ADEE16G02.2260.2260.3.dta 
               
               
                   
                 C07ADEE16G02.2828.2830.3.dta 
               
               
                   
                 0145ADEE17G02.2611.2613.2.dta 
               
               
                   
                 0145ADEE17G02.2617.2617.3.dta 
               
               
                   
                 0145ADEE17G02.3185.3187.3.dta 
               
             
          
           
               
                 Chain a, crystal structure of benzamidine inhibited bovine 
               
               
                 pancreatic trypsin at modelled 
               
             
          
           
               
                 patient: 
                 3 
               
               
                   
                 18ADEE16G02.1523.1527.2.dta 
               
               
                   
                 31ADEE16G02.1395.1397.2.dta 
               
               
                   
                 12ADEE15G02.1415.1415.2.dta 
               
               
                 control: 
                 2 
               
               
                   
                 0154ADEE16G02.1917.1917.2.dta 
               
               
                   
                 C07ADEE16G02.1523.1532.2.dta 
               
             
          
           
               
                 Complement component 5 [ homo sapiens ] 
               
             
          
           
               
                 patient: 
                 3 
               
               
                   
                 18ADEE16G02.3297.3299.3.dta 
               
               
                   
                 31ADEE16G02.3339.3341.3.dta 
               
               
                   
                 12ADEE15G02.3317.3325.3.dta 
               
               
                 control: 
                 2 
               
               
                   
                 C05ADEE16G02.3354.3356.3.dta 
               
               
                   
                 C07ADEE16G02.3354.3358.3.dta 
               
             
          
           
               
                 Ig heavy chain - human 
               
             
          
           
               
                 patient: 
                 1 
               
               
                   
                 18ADEE16G02.0768.0770.2.dta 
               
               
                   
                 31ADEE16G02.0768.0770.2.dta 
               
               
                 control: 
                 3 
               
               
                   
                 C05ADEE16G02.0774.0776.2.dta 
               
               
                   
                 C07ADEE16G02.0765.0767.2.dta 
               
               
                   
                 0154ADEE16G02.1176.1182.2.dta 
               
             
          
           
               
                 Lipoprotein ciii [ homo sapiens ] 
               
             
          
           
               
                 patient: 
                 1 
               
               
                   
                 18ADEE16G02.2158.2166.2.dta 
               
               
                 control: 
                 5 
               
               
                   
                 C05ADEE16G02.0953.0955.2.dta 
               
               
                   
                 C05ADEE16G02.2170.2178.2.dta 
               
               
                   
                 C07ADEE16G02.0948.0954.2.dta 
               
               
                   
                 0145ADEE17G02.2551.2555.2.dta 
               
               
                   
                 0145ADEE17G02.1356.1358.2.dta 
               
               
                   
                 0154ADEE16G02.1356.1358.2.dta 
               
               
                   
                 C05ADEE16G02.1356.1358.2.dta 
               
             
          
           
               
                 Vitronectin precursor (serum spreading factor) (s-protein) (v75) 
               
               
                 [contains: vitronectin v65 subunit; vitronectin v10 subunit; 
               
               
                 somatomedin b] 
               
             
          
           
               
                 patient: 
                 4 
               
               
                   
                 18ADEE16G02.1257.1257.3.dta 
               
               
                   
                 18ADEE16G02.2076.2076.2.dta 
               
               
                   
                 18ADEE16G02.1233.1233.3.dta 
               
               
                   
                 31ADEE16G02.1233.1233.3.dta 
               
               
                   
                 12ADEE15G02.1315.1321.2.dta 
               
               
                 control: 
                 3 
               
               
                   
                 0154ADEE16G02.1641.1645.3.dta 
               
               
                   
                 0145ADEE17G02.1251.1253.3.dta 
               
               
                   
                 C05ADEE16G02.1251.1253.3.dta 
               
               
                   
                 C07ADEE16G02.1257.1257.3.dta 
               
             
          
           
               
                 Common Single Ions Between Patient and Common Groups 
               
             
          
           
               
                 KTYSPAKYGK: 
                 gi|5174563 
               
               
                 patient: 
                 2 
               
               
                   
                 20ADEE16G02.0683.0685.2.dta 
               
               
                   
                 12ADEE15G02.0693.0695.2.dta 
               
               
                   
                 18ADEE16G02.0693.0695.2.dta 
               
               
                 common: 
                 2 
               
               
                   
                 12ADEE15G02.0659.0661.2.dta 
               
               
                   
                 0154ADEE16G02.1085.1087.2.dta 
               
               
                 MCDNCCKDSAFERTNITEYCR: 
                 gi|1172898 
               
               
                 patient: 
                 2 
               
               
                   
                 20ADEE16G02.1687.1687.3.dta 
               
               
                   
                 18ADEE16G02.1693.1693.3.dta 
               
               
                 common: 
                 3 
               
               
                   
                 C07ADEE16G02.1716.1718.3.dta 
               
               
                   
                 0154ADEE16G02.2084.2084.3.dta 
               
               
                   
                 0145ADEE17G02.2090.2096.3.dta 
               
             
          
           
               
                 Common Single Ions Between Control and Common Groups 
               
             
          
           
               
                 FKGYYKIAR: 
                 gi|17933636 
               
               
                 control: 
                 2 
               
               
                   
                 0154ADEE16G02.1272.1274.3.dta 
               
               
                   
                 0145ADEE17G02.1275.1281.3.dta 
               
               
                 common: 
                 2 
               
               
                   
                 C07ADEE16G02.0869.0873.3.dta 
               
               
                   
                 C05ADEE16G02.0872.0874.3.dta 
               
               
                 MAEMLVQLVRRIEK: 
                 gi|13385746 
               
               
                 control: 
                 2 
               
               
                   
                 C07ADEE16G02.0686.0688.3.dta 
               
               
                   
                 C05ADEE16G02.0689.0691.3.dta 
               
               
                 common: 
                 2 
               
               
                   
                 12ADEE15G02.0659.0661.3.dta 
               
               
                   
                 0154ADEE16G02.1085.1087.3.dta 
               
               
                   
               
             
          
         
       
     
     EXAMPLE 4 
     Unique Proteins in Patient Group 
     
       
         
               
             
               
             
               
             
               
             
               
             
               
             
           
               
                   
               
             
             
               
                 Proteins sharing keywords with the subtracted. 
               
             
          
           
               
                 (Format: Protein name and IDs: Keywords) 
               
               
                 gi|178853|gb|AAB5939 apolipoprotein e [ homo sapiens ] 
               
               
                 [mass = 36207]: apolipoprotein 
               
               
                 31ADEE16G02.2065.2067.2.dta 
               
               
                 12ADEE15G02.2002.2002.2.dta 
               
               
                 12ADEE15G02.0677.0679.2.dta 
               
               
                 31ADEE16G02.0677.0679.2.dta 
               
               
                 20ADEE16G02.1184.1190.2.dta 
               
               
                 18ADEE16G02.1201.1215.2.dta 
               
               
                 31ADEE16G02.2061.2063.3.dta 
               
               
                 18ADEE16G02.1361.1361.2.dta 
               
               
                 18ADEE16G02.1028.1028.2.dta 
               
               
                 31ADEE16G02.1028.1030.2.dta 
               
               
                 31ADEE16G02.2007.2011.2.dta 
               
               
                 12ADEE15G02.0781.0781.2.dta 
               
               
                 31ADEE16G02.0781.0781.2.dta 
               
               
                 12ADEE15G02.1015.1015.2.dta 
               
               
                 20ADEE16G02.1015.1017.2.dta 
               
               
                 18ADEE16G02.1990.1996.2.dta 
               
               
                 20ADEE16G02.1350.1352.2.dta 
               
               
                 18ADEE16G02.0719.0725.2.dta 
               
               
                 18ADEE16G02.0689.0689.2.dta 
               
               
                 31ADEE16G02.0685.0689.2.dta 
               
               
                 18ADEE16G02.2048.2056.2.dta 
               
               
                 12ADEE15G02.0781.0781.2.dta 
               
               
                 31ADEE16G02.0781.0781.2.dta 
               
               
                 12ADEE15G02.1011.1013.2.dta 
               
               
                 20ADEE16G02.1013.1013.2.dta 
               
               
                 18ADEE16G02.2046.2050.3.dta 
               
               
                 12ADEE15G02.2000.2000.2.dta 
               
               
                 12ADEE15G02.0867.0867.2.dta 
               
               
                 18ADEE16G02.0867.0869.2.dta 
               
               
                 20ADEE16G02.0867.0869.2.dta 
               
             
          
           
               
                 The Rest of the Unique Proteins. 
               
             
          
           
               
                 gi|577055|emb|CAA249: gamma-fibrinogen chain fragment [ homo   
               
               
                   sapiens ] [mass = 7031] 
               
               
                 18ADEE16G02.1453.1461.2.dta 
               
               
                 31ADEE16G02.1427.1429.2.dta 
               
               
                 12ADEE15G02.1439.1455.2.dta 
               
               
                 20ADEE16G02.1440.1442.2.dta 
               
               
                 gi|17066105|emb|CAD1: titin [ homo sapiens ] [mass = 3816218] 
               
               
                 12ADEE15G02.0948.0950.2.dta 
               
               
                 20ADEE16G02.0941.0943.2.dta 
               
               
                 12ADEE15G02.0937.0939.2.dta 
               
               
                 18ADEE16G02.0937.0939.2.dta 
               
               
                 31ADEE16G02.0937.0939.2.dta 
               
               
                 18ADEE16G02.1565.1572.3.dta 
               
               
                 31ADEE16G02.1913.1915.3.dta 
               
               
                 12ADEE15G02.0532.0534.3.dta 
               
               
                 18ADEE16G02.1755.1761.3.dta 
               
               
                 12ADEE15G02.1260.1262.3.dta 
               
               
                 31ADEE16G02.1089.1119.2.dta 
               
               
                 12ADEE15G02.1556.1558.2.dta 
               
               
                 20ADEE16G02.1556.1558.2.dta 
               
             
          
           
               
                 Summary list, refer above for IDs and DTA files. 
               
             
          
           
               
                 apolipoprotein e [ homo sapiens ] [mass = 36207] 
               
               
                 gamma-fibrinogen chain fragment [ homo sapiens ] [mass = 7031] 
               
               
                 titin [ homo sapiens ] [mass = 3816218] 
               
               
                   
               
             
          
         
       
     
     EXAMPLE 5 
     Unique Single Ions in Patient Group 
     
       
         
               
             
               
               
               
             
           
               
                   
               
               
                 Unique Single Ions in Patient Group, With Multiple Sample 
               
               
                 Matches For Peptides 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                   
                 2: 
                 AADDTWEPFASGK gi|2135953 
               
               
                   
                   
                 12ADEE15G02.1383.1383.2.dta 
               
               
                   
                   
                 18ADEE16G02.1391.1395.2.dta 
               
               
                   
                 2: 
                 AAKGAKPEPAPAPPPPGAK gi|18598655 
               
               
                   
                   
                 31ADEE16G02.0826.0828.3.dta 
               
               
                   
                   
                 20ADEE16G02.0833.0835.3.dta 
               
               
                   
                 2: 
                 AEDTAVYYCARDIQLDAFDIWGQ gi|13171474 
               
               
                   
                   
                 18ADEE16G02.1411.1411.3.dta 
               
               
                   
                   
                 20ADEE16G02.1411.1411.3.dta 
               
               
                   
                   
                 31ADEE16G02.1411.1411.3.dta 
               
               
                   
                   
                 18ADEE16G02.1425.1427.3.dta 
               
               
                   
                 2: 
                 AETVIIGGGCVGVSLAYHLAKAGMK gi|7019365 
               
               
                   
                   
                 20ADEE16G02.1937.1943.3.dta 
               
               
                   
                   
                 31ADEE16G02.1949.1951.3.dta 
               
               
                   
                 2: 
                 APAAAEMLLRSKS gi|17451900 
               
               
                   
                   
                 31ADEE16G02.2297.2347.2.dta 
               
               
                   
                   
                 12ADEE15G02.2304.2306.2.dta 
               
               
                   
                   
                 18ADEE16G02.2304.2306.2.dta 
               
               
                   
                 2: 
                 APLARLHPPIK gi|21244563 
               
               
                   
                   
                 18ADEE16G02.2376.2380.1.dta 
               
               
                   
                   
                 31ADEE16G02.2399.2401.1.dta 
               
               
                   
                 2: 
                 APWIPPNPTSPLASPKCAAWLKVK gi|7512882 
               
               
                   
                   
                 12ADEE15G02.0937.0939.3.dta 
               
               
                   
                   
                 18ADEE16G02.0937.0939.3.dta 
               
               
                   
                   
                 31ADEE16G02.0937.0939.3.dta 
               
               
                   
                   
                 20ADEE16G02.0941.0943.3.dta 
               
               
                   
                 2: 
                 CCGGTVIQTQACTPSR gi|18584772 
               
               
                   
                   
                 20ADEE16G02.1060.1062.3.dta 
               
               
                   
                   
                 18ADEE16G02.1071.1079.3.dta 
               
               
                   
                 2: 
                 DINYVNPVIK gi|14735486 
               
               
                   
                   
                 20ADEE16G02.1231.1235.2.dta 
               
               
                   
                   
                 12ADEE15G02.1240.1240.2.dta 
               
               
                   
                 2: 
                 DKEFLVFETSDIAISLKWLVEDIK gi|12045184 
               
               
                   
                   
                 20ADEE16G02.2495.2497.3.dta 
               
               
                   
                   
                 31ADEE16G02.2549.2551.3.dta 
               
               
                   
                 3: 
                 DKVILLDETLNAIATELKPR gi|13508310 
               
               
                   
                   
                 31ADEE16G02.0843.0853.3.dta 
               
               
                   
                   
                 12ADEE15G02.0851.0855.3.dta 
               
               
                   
                   
                 18ADEE16G02.0871.0873.3.dta 
               
               
                   
                   
                 20ADEE16G02.0871.0873.3.dta 
               
               
                   
                 2: 
                 DSALSTPRAELAQQWFVLALR gi|21231993 
               
               
                   
                   
                 20ADEE16G02.1174.1176.3.dta 
               
               
                   
                   
                 12ADEE15G02.1188.1192.3.dta 
               
               
                   
                 2: 
                 DVEGDSVPK gi|20482245 
               
               
                   
                   
                 31ADEE16G02.0817.0822.2.dta 
               
               
                   
                   
                 18ADEE16G02.0827.0829.2.dta 
               
               
                   
                   
                 20ADEE16G02.0827.0829.2.dta 
               
               
                   
                 2: 
                 DVWGIEGPIDAAFTR gi|139653 
               
               
                   
                   
                 20ADEE16G02.2049.2053.2.dta 
               
               
                   
                   
                 31ADEE16G02.2083.2087.2.dta 
               
               
                   
                 2: 
                 DYWSTVK gi|224917 
               
               
                   
                   
                 31ADEE16G02.1034.1036.1.dta 
               
               
                   
                   
                 12ADEE15G02.1054.1060.2.dta 
               
               
                   
                 2: 
                 EGAGSSALK gi|10436629 
               
               
                   
                   
                 31ADEE16G02.0904.0908.2.dta 
               
               
                   
                   
                 20ADEE16G02.0915.0917.2.dta 
               
               
                   
                 2: 
                 EHSGTRLHPCTRCTVK gi|20548673 
               
               
                   
                   
                 31ADEE16G02.1762.1766.2.dta 
               
               
                   
                   
                 18ADEE16G02.1773.1773.2.dta 
               
               
                   
                 2: 
                 ENLSPEDFK gi|15668768 
               
               
                   
                   
                 12ADEE15G02.0722.0722.2.dta 
               
               
                   
                   
                 20ADEE16G02.0729.0735.2.dta 
               
               
                   
                 2: 
                 EPAEKPGLGR gi|11498871 
               
               
                   
                   
                 20ADEE16G02.0939.0945.2.dta 
               
               
                   
                   
                 18ADEE16G02.0962.0964.2.dta 
               
               
                   
                 2: 
                 EQGISLANVVPHK gi|7435945 
               
               
                   
                   
                 31ADEE16G02.0813.0813.3.dta 
               
               
                   
                   
                 12ADEE15G02.0819.0819.3.dta 
               
               
                   
                   
                 20ADEE16G02.0819.0819.3.dta 
               
               
                   
                 2: 
                 GPEGIGKPGAAGAPGQPGIPGTK gi|18105032 
               
               
                   
                   
                 20ADEE16G02.1440.1442.3.dta 
               
               
                   
                   
                 18ADEE16G02.1453.1461.3.dta 
               
               
                   
                 2: 
                 GQPKDATDWCCQKHDCCYAHLK gi|7242177 
               
               
                   
                   
                 18ADEE16G02.2382.2386.3.dta 
               
               
                   
                   
                 31ADEE16G02.2393.2395.3.dta 
               
               
                   
                 2: 
                 GSPAINVAVHVFR gi|1336728 
               
               
                   
                   
                 20ADEE16G02.1354.1356.2.dta 
               
               
                   
                   
                 12ADEE15G02.1356.1356.2.dta 
               
               
                   
                 2: 
                 GWVTDGFSSLK gi|224917 
               
               
                   
                   
                 12ADEE15G02.1590.1594.2.dta 
               
               
                   
                   
                 12ADEE15G02.1592.1592.1.dta 
               
               
                   
                 2: 
                 HQLVVSSPPR gi|14783228 
               
               
                   
                   
                 18ADEE16G02.1737.1745.2.dta 
               
               
                   
                   
                 20ADEE16G02.1743.1743.2.dta 
               
               
                   
                 2: 
                 IDDLFDQLKGARVFSK gi|6002795 
               
               
                   
                   
                 20ADEE16G02.1821.1823.3.dta 
               
               
                   
                   
                 18ADEE16G02.1833.1835.3.dta 
               
               
                   
                   
                 20ADEE16G02.1833.1835.3.dta 
               
               
                   
                 2: 
                 IELEHSIGAIQHSLSR gi|16077314 
               
               
                   
                   
                 20ADEE16G02.1436.1438.3.dta 
               
               
                   
                   
                 18ADEE16G02.1447.1447.3.dta 
               
               
                   
                 2: 
                 KAVVARK gi|9827811 
               
               
                   
                   
                 12ADEE15G02.0929.0929.2.dta 
               
               
                   
                   
                 20ADEE16G02.0949.0949.2.dta 
               
               
                   
                   
                 31ADEE16G02.0949.0949.2.dta 
               
               
                   
                 2: 
                 KIILTESYCKSELCILSTQTR gi|18595923 
               
               
                   
                   
                 12ADEE15G02.0781.0781.3.dta 
               
               
                   
                   
                 31ADEE16G02.0781.0781.3.dta 
               
               
                   
                   
                 20ADEE16G02.0792.0794.3.dta 
               
               
                   
                 2: 
                 KSIKIDDLK gi|16804979 
               
               
                   
                   
                 18ADEE16G02.0845.0845.2.dta 
               
               
                   
                   
                 20ADEE16G02.0845.0845.2.dta 
               
               
                   
                   
                 18ADEE16G02.0853.0853.2.dta 
               
               
                   
                 2: 
                 LAAQLFSNTTANAIR gi|422517 
               
               
                   
                   
                 31ADEE16G02.1163.1171.2.dta 
               
               
                   
                   
                 12ADEE15G02.1176.1210.2.dta 
               
               
                   
                 2: 
                 LAMRGYPMLTTMCPGPQKK gi|20546026 
               
               
                   
                   
                 12ADEE15G02.0821.0825.3.dta 
               
               
                   
                   
                 18ADEE16G02.0835.0839.3.dta 
               
               
                   
                 2: 
                 LGALNSSLQLLEDRLHQLSLK gi|14043093 
               
               
                   
                   
                 12ADEE15G02.0795.0795.3.dta 
               
               
                   
                   
                 31ADEE16G02.0795.0795.3.dta 
               
               
                   
                   
                 20ADEE16G02.0798.0800.3.dta 
               
               
                   
                 2: 
                 LHEMNKHEETVSVAMR gi|20468266 
               
               
                   
                   
                 12ADEE15G02.1040.1040.3.dta 
               
               
                   
                   
                 31ADEE16G02.1040.1040.3.dta 
               
               
                   
                   
                 20ADEE16G02.1049.1058.3.dta 
               
               
                   
                 2: 
                 LIEPICPQFAEYVWRK gi|8569090 
               
               
                   
                   
                 20ADEE16G02.1709.1709.2.dta 
               
               
                   
                   
                 12ADEE15G02.1723.1723.2.dta 
               
               
                   
                 2: 
                 LIQLKPQEEETHDEIFGGLSKVHQK gi|20482517 
               
               
                   
                   
                 18ADEE16G02.1931.1931.3.dta 
               
               
                   
                   
                 12ADEE15G02.1934.1934.3.dta 
               
               
                   
                 2: 
                 LLLNKSASAQNELMLLDMLK gi|19115107 
               
               
                   
                   
                 20ADEE16G02.1013.1013.3.dta 
               
               
                   
                   
                 12ADEE15G02.1015.1015.3.dta 
               
               
                   
                 2: 
                 LMDQNLK gi|14010352 
               
               
                   
                   
                 12ADEE15G02.0915.0915.1.dta 
               
               
                   
                   
                 20ADEE16G02.0919.0919.1.dta 
               
               
                   
                 2: 
                 LQKAIEEEEARMR gi|11360154 
               
               
                   
                   
                 20ADEE16G02.0927.0929.3.dta 
               
               
                   
                   
                 18ADEE16G02.0943.0945.3.dta 
               
               
                   
                   
                 31ADEE16G02.0943.0945.3.dta 
               
               
                   
                 2: 
                 LSDAEPEVR gi|20480773 
               
               
                   
                   
                 20ADEE16G02.0776.0778.2.dta 
               
               
                   
                   
                 18ADEE16G02.0784.0786.2.dta 
               
               
                   
                 2: 
                 LSILTEK gi|20546459 
               
               
                   
                   
                 12ADEE15G02.0564.0566.1.dta 
               
               
                   
                   
                 20ADEE16G02.0634.0636.1.dta 
               
               
                   
                 2: 
                 LTLIEKPCPR gi|18558922 
               
               
                   
                   
                 20ADEE16G02.1663.1665.2.dta 
               
               
                   
                   
                 18ADEE16G02.1677.1681.2.dta 
               
               
                   
                 2: 
                 MTSSFHGIRPPQLEQPEKMPVLK gi|12839853 
               
               
                   
                   
                 20ADEE16G02.1273.1275.3.dta 
               
               
                   
                   
                 12ADEE15G02.1280.1282.3.dta 
               
               
                   
                 2: 
                 NAMRVTLDATGNEGSWLFIQPFWK gi|17366458 
               
               
                   
                   
                 12ADEE15G02.0805.0815.3.dta 
               
               
                   
                   
                 18ADEE16G02.0814.0820.3.dta 
               
               
                   
                 2: 
                 NGDDSDNGDGADVK gi|11061640 
               
               
                   
                   
                 20ADEE16G02.1281.1281.3.dta 
               
               
                   
                   
                 12ADEE15G02.1287.1289.3.dta 
               
               
                   
                 2: 
                 NIAQFGEMIRLKTGR gi|346690 
               
               
                   
                   
                 18ADEE16G02.2124.2124.2.dta 
               
               
                   
                   
                 12ADEE15G02.2134.2138.2.dta 
               
               
                   
                 2: 
                 NIHFSWDILAK gi|11078753 
               
               
                   
                   
                 12ADEE15G02.2294.2298.1.dta 
               
               
                   
                   
                 18ADEE16G02.2318.2322.1.dta 
               
               
                   
                 2: 
                 NLLENLSGGSTFK gi|2129238 
               
               
                   
                   
                 31ADEE16G02.0748.0748.2.dta 
               
               
                   
                   
                 18ADEE16G02.0761.0761.2.dta 
               
               
                   
                 2: 
                 NPAIFGFLLSAK gi|6319547 
               
               
                   
                   
                 12ADEE15G02.1025.1027.3.dta 
               
               
                   
                   
                 12ADEE15G02.1044.1046.3.dta 
               
               
                   
                   
                 18ADEE16G02.1044.1046.3.dta 
               
               
                   
                 2: 
                 PLTTSPAPAPPPR gi|14747828 
               
               
                   
                   
                 20ADEE16G02.1047.1053.2.dta 
               
               
                   
                   
                 12ADEE15G02.1052.1056.2.dta 
               
               
                   
                 2: 
                 PSGNSSSGGKIWQGEPARVR gi|5453900 
               
               
                   
                   
                 31ADEE16G02.0748.0748.3.dta 
               
               
                   
                   
                 18ADEE16G02.0761.0761.3.dta 
               
               
                   
                 2: 
                 PSPQRNTTNDIAHIQNEEIMSLQMK gi|20554181 
               
               
                   
                   
                 20ADEE16G02.1910.1925.3.dta 
               
               
                   
                   
                 12ADEE15G02.1922.1924.3.dta 
               
               
                   
                 2: 
                 QDFTVKTVSGER gi|20470381 
               
               
                   
                   
                 12ADEE15G02.0807.0809.3.dta 
               
               
                   
                   
                 20ADEE16G02.0807.0809.3.dta 
               
               
                   
                   
                 18ADEE16G02.0812.0812.3.dta 
               
               
                   
                 2: 
                 QDMIVRTTQEKLK gi|20070257 
               
               
                   
                   
                 18ADEE16G02.0845.0845.3.dta 
               
               
                   
                   
                 20ADEE16G02.0845.0845.3.dta 
               
               
                   
                   
                 18ADEE16G02.0853.0853.3.dta 
               
               
                   
                 4: 
                 QIFSILAATPGVQVEK gi|11061648 
               
               
                   
                   
                 31ADEE16G02.1087.1097.3.dta 
               
               
                   
                   
                 12ADEE15G02.1108.1110.3.dta 
               
               
                   
                   
                 20ADEE16G02.1112.1116.3.dta 
               
               
                   
                   
                 18ADEE16G02.1126.1136.3.dta 
               
               
                   
                 2: 
                 QPTQEEILFQVAQAGEVDK gi|1708635 
               
               
                   
                   
                 20ADEE16G02.1156.1160.3.dta 
               
               
                   
                   
                 12ADEE15G02.1167.1169.3.dta 
               
               
                   
                 2: 
                 QPVPAGVVPGVVYER gi|21233559 
               
               
                   
                   
                 18ADEE16G02.1833.1835.3.dta 
               
               
                   
                   
                 20ADEE16G02.1833.1835.3.dta 
               
               
                   
                   
                 12ADEE15G02.1840.1840.3.dta 
               
               
                   
                 2: 
                 QTDEQLHRMGAPR gi|21241051 
               
               
                   
                   
                 20ADEE16G02.1739.1741.2.dta 
               
               
                   
                   
                 31ADEE16G02.1853.1855.2.dta 
               
               
                   
                 2: 
                 QYEQNIMDHKLNLDLLTQSTSSSR gi|20536830 
               
               
                   
                   
                 18ADEE16G02.1656.1662.3.dta 
               
               
                   
                   
                 12ADEE15G02.1661.1661.3.dta 
               
               
                   
                 2: 
                 RDRAPFVFTSDMAYVINGGDK gi|13632400 
               
               
                   
                   
                 31ADEE16G02.0621.0623.3.dta 
               
               
                   
                   
                 18ADEE16G02.0637.0639.3.dta 
               
               
                   
                 2: 
                 RIADLRSDLGPEIR gi|21230680 
               
               
                   
                   
                 12ADEE15G02.1731.1739.1.dta 
               
               
                   
                   
                 18ADEE16G02.1731.1757.1.dta 
               
               
                   
                 4: 
                 SKEQLTPLIK gi|4502149 
               
               
                   
                   
                 31ADEE16G02.0912.0914.2.dta 
               
               
                   
                   
                 20ADEE16G02.0933.0935.2.dta 
               
               
                   
                   
                 20ADEE16G02.0949.0949.3.dta 
               
               
                   
                   
                 31ADEE16G02.0949.0949.3.dta 
               
               
                   
                   
                 18ADEE16G02.0949.0952.3.dta 
               
               
                   
                 2: 
                 SLPWSAARQLR gi|21231002 
               
               
                   
                   
                 12ADEE15G02.0439.0441.3.dta 
               
               
                   
                   
                 31ADEE16G02.0499.0501.3.dta 
               
               
                   
                 2: 
                 SLSLAQNLLER gi|18560704 
               
               
                   
                   
                 20ADEE16G02.1074.1096.2.dta 
               
               
                   
                   
                 18ADEE16G02.1091.1120.2.dta 
               
               
                   
                 3: 
                 SPELQAEAK gi|4502149 
               
               
                   
                   
                 12ADEE15G02.0612.0614.2.dta 
               
               
                   
                   
                 20ADEE16G02.0612.0614.2.dta 
               
               
                   
                   
                 12ADEE15G02.0616.0618.1.dta 
               
               
                   
                   
                 31ADEE16G02.0635.0637.1.dta 
               
               
                   
                 4: 
                 SSGTSYPDVLK gi|15988427 
               
               
                   
                   
                 31ADEE16G02.0975.0977.2.dta 
               
               
                   
                   
                 20ADEE16G02.0987.0989.2.dta 
               
               
                   
                   
                 12ADEE15G02.0991.0993.2.dta 
               
               
                   
                   
                 18ADEE16G02.1004.1010.2.dta 
               
               
                   
                 2: 
                 SSQSVLYSSNSK gi|1730075 
               
               
                   
                   
                 18ADEE16G02.1992.1994.2.dta 
               
               
                   
                   
                 31ADEE16G02.2013.2015.2.dta 
               
               
                   
                 3: 
                 SSSRPKVSATAAAAAGK gi|8250399 
               
               
                   
                   
                 20ADEE16G02.1019.1021.2.dta 
               
               
                   
                   
                 12ADEE15G02.1038.1038.1.dta 
               
               
                   
                   
                 18ADEE16G02.1040.1042.2.dta 
               
               
                   
                 2: 
                 TIENSQCTKVEEDFNLATKIISK gi|13469731 
               
               
                   
                   
                 31ADEE16G02.2254.2256.3.dta 
               
               
                   
                   
                 20ADEE16G02.2262.2266.3.dta 
               
               
                   
                 2: 
                 TQLQSFSQYIENRPEMK gi|17441098 
               
               
                   
                   
                 31ADEE16G02.1445.1457.3.dta 
               
               
                   
                   
                 12ADEE15G02.1451.1453.3.dta 
               
               
                   
                 2: 
                 VAVARAQGLGGQLR gi|21231008 
               
               
                   
                   
                 18ADEE16G02.2338.2342.2.dta 
               
               
                   
                   
                 31ADEE16G02.2371.2371.2.dta 
               
               
                   
                 3: 
                 VEGNEVIIENFIGEK gi|11499493 
               
               
                   
                   
                 18ADEE16G02.2280.2282.2.dta 
               
               
                   
                   
                 31ADEE16G02.2285.2287.2.dta 
               
               
                   
                   
                 31ADEE16G02.2291.2311.1.dta 
               
               
                   
                 4: 
                 VKSPELQAEAK gi|4502149 
               
               
                   
                   
                 12ADEE15G02.0645.0647.2.dta 
               
               
                   
                   
                 12ADEE15G02.0651.0653.2.dta 
               
               
                   
                   
                 31ADEE16G02.0651.0653.2.dta 
               
               
                   
                   
                 20ADEE16G02.0658.0660.2.dta 
               
               
                   
                   
                 18ADEE16G02.0667.0669.2.dta 
               
               
                   
                   
                 31ADEE16G02.0667.0669.2.dta 
               
               
                   
                 3: 
                 VPGPAFGHQIAYCNLLPR gi|20538746 
               
               
                   
                   
                 31ADEE16G02.0881.0885.3.dta 
               
               
                   
                   
                 20ADEE16G02.0891.0895.3.dta 
               
               
                   
                   
                 18ADEE16G02.0904.0906.3.dta 
               
               
                   
                 2: 
                 VYVSLGALKMHIRTHTLPCK gi|399449 
               
               
                   
                   
                 31ADEE16G02.2469.2505.3.dta 
               
               
                   
                   
                 18ADEE16G02.2482.2486.3.dta 
               
               
                   
                 2: 
                 YIHTGEK gi|13374557 
               
               
                   
                   
                 20ADEE16G02.0893.0893.2.dta 
               
               
                   
                   
                 12ADEE15G02.0893.0895.2.dta 
               
               
                   
                 2: 
                 YWITADK gi|19115051 
               
               
                   
                   
                 12ADEE15G02.1042.1050.1.dta 
               
               
                   
                   
                 18ADEE16G02.1057.1059.1.dta 
               
               
                   
                 6: 
                 Apolipoprotein a-ii precursor [ homo sapiens ] 
               
               
                   
                   
                 12ADEE15G02.0925.0927.2.dta 
               
               
                   
                   
                 20ADEE16G02.2683.2685.2.dta 
               
               
                   
                   
                 31ADEE16G02.0659.0663.3.dta 
               
               
                   
                   
                 31ADEE16G02.2745.2759.2.dta 
               
               
                   
                   
                 31ADEE16G02.2753.2765.3.dta 
               
               
                   
                   
                 31ADEE16G02.2904.2918.3.dta 
               
               
                   
                 1: 
                 C9 complement protein [ homo sapiens ] 
               
               
                   
                   
                 18ADEE16G02.1799.1799.2.dta 
               
               
                   
                 1: 
                 Chain c, crystal structure of the human 
               
               
                   
                   
                 alpha-thrombin-haemadin complex: 
               
               
                   
                   
                 an exosite ii-binding inhibitor 
               
               
                   
                   
                 20ADEE16G02.1436.1438.2.dta 
               
               
                   
                 1: 
                 Gvp1 gene in phh1 homolog [ rhizobium rhizogenes ] 
               
               
                   
                   
                 18ADEE16G02.1749.1751.3.dta 
               
               
                   
                 1: 
                 Prothrombin [ homo sapiens ] 
               
               
                   
                   
                 20ADEE16G02.1401.1405.2.dta 
               
               
                   
                   
                 31ADEE16G02.1401.1405.2.dta 
               
               
                   
                   
               
             
          
         
       
     
     The examples illustrate the summarization of Group 1 _unique with_name; Group 2  unique_with_name; Group 1 _unique_without_name and Group 2 _unique_without_name; wherein Group_unique_without name list is used for optional databases searches, and Group unique_with_name list is used directly for further analysis such as data mining. 
     It is to be understood that while we have illustrated and described certain forms of our invention, it is not to be limited to the specific forms or arrangement of the steps herein described and shown. It will be apparent to those skilled in the art that various changes may be made without departing from the scope of the invention and the invention is not to be considered limited to what is shown in the drawings and described in the specification. 
     Although the instant invention has been exemplified in the context of an ion-trap device, it is to be understood that the sample mass may be analyzed by any combination of ionization and mass spectrometer. The ionization method may include, but is not limited to such methods as electron ionization, chemical ionization, fast atom bombardment, desorption chemical ionization, plasmadeorption, thermospray, atmospheric pressure chemical ionization, MALDI or electrospray ionization (ESI) Principally the ionization method will be MALDI or ESI. 
     Prior art mass spectrometer formats are known to be useful either singly or in various combinations for use in analyzing translation products. These formats may include, but are not limited to, ionization (I) techniques, including but not limited to matrix assisted laser desorption (MALDI), continuous or pulsed electrospray (ESI) and related methods (e.g., IONSPRAY or THERMOSPRAY), or massive cluster impact (MCI); these ion sources can be matched with detection formats including linear or non-linear reflection time-of-flight (TOF), single or multiple quadropole, single or multiple magnetic sector, Fourier Transform ion cyclotron resonance (FTICR), ion trap, and combinations thereof (e.g., ion-trap/time-of-flight). For ionization, numerous matrix/wavelength combinations (MALDI) or solvent combinations (ESI) can be employed. Subattomole levels of protein have been detected, for example, using ESI (Valaskovic, G. A. et al., (1996) Science 273:1199-1202) or MALDI (Li, L. et al., (1996) J. Am. Chem. Soc. 118:1662-1663) mass spectrometry. ES mass spectrometry has been introduced by Fenn et al. (J. Phys. Chem. 88, 4451-59 (1984); PCT Application No. WO 90/14148) and current applications are summarized in recent review articles (R. D. Smith et al., Anal. Chem. 62, 882-89 (1990) and B. Ardrey, Electrospray Mass Spectrometry, Spectroscopy Europe, 4, 10-18 (1992)). MALDI-TOF mass spectrometry has been introduced by Hillenkamp et al. (“Matrix Assisted UV-Laser Desorption/Ionization: A New Approach to Mass Spectrometry of Large Biomolecules,” Biological Mass Spectrometry (Burlingame and McCloskey, editors), Elsevier Science Publishers, Amsterdam, pp. 49-60, 1990). With ESI, the determination of molecular weights in femtomole amounts of sample is very accurate due to the presence of multiple ion peaks which all could be used for the mass calculation. 
     With regard to electrospray, an electrospray is produced by applying a strong electric field under atmospheric pressure to a liquid passing through a capillary tube with a weak flux. The electric field is obtained by applying a potential difference of 3-6 kV between the capillary and the counter electrode separate by 0.3 to 2 cm producing electric fields of the order of 10 E6 V per cm. This field induces a charge at the liquid surface located at the end of capillary, which will break to form highly charged droplets. A gas injected co-axially at a low flow rate allows the dispersion of the spray to be limited in space. These droplets then pass either through a curtain of heated inert gas, most often nitrogen, or through a heated capillary to remove the last solvent molecules.” See for example “Mass spectrometry, principle and application” 2nd edition. 2001. Edmond de Hoffmann and Vincent Stroobant. John Wiley &amp; Sons, New York. Page 35. 
     With regard to the mass spectrometer, per se, there are several types of tandem mass spectrometers that could be used to generate the patterns of MS/MS spectra that are the substrate for the program. These mass spectrometers generate both parent ions in the +1 (MALDI and ESI) and the +2, +3, +4 etc charge state (ESI). The program takes the charge state of the parent into consideration as part of the data associated with the parent ion. The types of mass analyzer mass analyzers include the quadropole, octopole, ion trap or QUISTOR, time of flight (TOF) Time of Flight with reflectron, the Fourier transformed ion cyclotron resonance (FTICR also called FTMS), magnetic and electromagnetic sector. The tandem mass spectrometer mass consist of the homogenous or heterogenous combination of two or more of any of these devices in any order (Hybrid instrument). The fragmentation method may include post source decay of the parent ion or its fragment, or collision-activated or collision-induced decomposition of the parent ions, or fragment ions, or metastable ions with a relatively immobile target such as gas molecules or other parent or fragment ions, or photons or electrons or solid objects. The combinations may preferentially include the quadropole or octopole ion trap, the Quadropole-Quadropole (DC and rf or rf only)-TOF, the ion trap-FTMS, the TOF-TOF, the ion trap-TOF, or the TOF-PSD-TOF. 
     The signal resulting from the instrument may be mathematically transformed or filtered. The MASS analyzer may be directly or indirectly coupled to a number of devices, which are illustrated by, but not limited to, liquid chromatography or capillary electrophoresis, or free flow electrophoresis, or gel electrophoresis, or multidimensional chromatography, or multidimensional electrophoresis.