Abstract:
The invention pertains to a near-infrared fluorescent dye that is cell permeable and can be attached to selected proteins in living cells. The dye has the general formula 
     
       
                 
         
             
             
         
       
     
     or its corresponding spiro ac one 
     
       
                 
         
             
             
         
       
     
     wherein
 
Y is chosen from the group consisting of Si, Ge and Sn;
 
R 0  is —COO −  or COOH;
 
R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , R 10 , R 11 , R 12 , R 13 , R 15  and R 16  are substituents, including hydrogen, independently from each other.
 
     The dye (i) absorbs and emits light at wavelengths above 600 nm; (ii) possesses high photostability; (iii) has high extinction coefficients and high quantum yields; (iv) can be derivatized with different molecules; and (v) is membrane-permeable and shows minimal background binding to biomolecules and biomolecular structures.

Description:
[0001]    The invention pertains to the field of fluorescent dyes, in particular to cell permeable fluorescent dyes. 
       BACKGROUND OF THE INVENTION 
       [0002]    Synthetic fluorophores are important tools in chemistry and biology. One of the main applications is their use as molecular probes in biomolecular imaging (Lavin, L. D.; Raines, R. T. ACS Chem Biol 2008, 3, 142). The ideal fluorophore for applications in biomolecular imaging should fulfill at least the following five criteria: First, the fluorophore absorbs and admits light at long wavelengths, preferentially above 600 nm. This ensures minimal phototoxicity when exciting the fluorophore, reduces background from cellular autofluorescence and increases tissue penetration for in vivo applications. Second, the fluorophore should possess high photostability to avoid rapid bleaching in the course of an experiment. Third, the fluorophore should be very bright, that is it should possess high extinction coefficients and high quantum yields. Fourth, a derivatization of the fluorophore with (i) reactive groups such as activated esters, (ii) ligands that specifically bind to other (bio)molecules in vitro or in vivo or (iii) molecules that can control the fluorescence properties of the fluorophore should be possible. Fifth, the fluorophore should be membrane permeable and show minimal background binding to biomolecules and biomolecular structures. While numerous fluorophores exist that fulfill the first four criteria, the cyanine fluorophore Cy5 being an example, there are few fluorophores available that also fulfill the fifth criterion. 
         [0003]    Recently, a new class of fluorophores have been introduced which are based on the rhodamine structure but in which the oxygen atom in the xanthene ring has been replaced by silicon (Si-rhodamine) or germanium (Ge-rhodamine); cf.  FIG. 1  (Xiao, Y.; Fu, M. 2008; CN 1810812. Fu, M.; Xiao, Y.; Qian, X.; Zhao, D.; Xu, Y. Chem Commun (Camb) 2008, 1780. Nagano, T.; Urano, Y.; Koide, Y, 2010; WO 2010126077, p 35. Koide, Y.; Urano, Y.; Hanaoka, K.; Terai, T.; Nagano, T. ACS Chem Biol 2011, 6, 600. Koide, Y.; Urano, Y.; Hanaoka K.; Terai, T.; Nagano, T. J Am Chem Sac 2011, 133, 5680. Egawa, T.; Koide, Y.; Hanaoka, K,; Komatsu, T.; Terai, T.; Nagano, T. Chem Commun (Camb) 2011, 47, 4162): 
         [0000]    
       
                 
         
             
             
         
       
     
         [0004]    These fluorophores show a large bathochromic shift relative to regular rhodamine derivatives with excitation and emission wavelengths above 600 nm. At the same time, they have high solubility, are very bright and photostable. In addition, there have been reports on their use in bimolecular imaging (Koide, Y.; Urano, Y.; Hanaoka, K.; Terai, T,; Nagano, T. ACS Chem Biol 2011, 6, 600. Koide, Y.; Urano, Y.; Hanaoka, K.; Terai, T.; Nagano, T. J Am Chem Soc 2011, 133, 5680. Egawa, T.; Koide, Y.; Hanaoka, K.; Komatsu, T.; Terai, T.; Nagano, T. Chem Commun (Camb) 2011, 47, 4162, Egawa, T. et al., J. Am. Chem. Soc, Epub ahead of print, DCI: 10.1021/ja205809h). 
         [0005]    The specific coupling of fluorophores to proteins in living cells is an important method in life sciences. Such a specific coupling of fluorophores can be achieved by expressing the protein of interest as a fusion protein with an additional polypeptide that mediates the labeling of the fusion protein with the fluorophore (for review on labelling methods cf. Hinner, M. J.; Johnsson, K. Curr Opin Biotechnol 2010, 21, 766). Numerous approaches exist for achieving such a specific labeling in vitro and in vivo. Examples for such tags are small peptides that tightly bind to other molecules, proteins that tightly bind to other molecules, proteins that undergo a covalent reaction with other molecules and peptides to which other molecules are coupled with the help of enzymes. Methods that have been shown of particular utility for the labeling of intracellular protein are the tetracysteine tag that binds to biarsenical fluorophores, the SNAP-tag that irreversibly reacts with benzylguanine (BG) derivatives (Keppler, A.; Gendreizig, S.; Gronemeyer, T.; Pick, H.; Vogel, H.; Johnsson, K. Nat Biotechnol 2003, 21, 86), the CLIP-tag that reacts with benzylcytosine derivatives, the Halo-tag that reacts with primary chlorides and dihydrofolate reductase that binds to trimethoprim derivatives (Hinner, M. J.; Johnsson, K. Curr Opin Biotechnol 2010, 21, 766). Alternatively, a specific fluorescence labeling of a protein of interest can be achieved through the incorporation of an unnatural, fluorescent amino acid (Liu, C. C.; Schultz, P. G. Annu Rev Biochem 2010, 79, 413). While numerous fluorophores with excitation and emission maxima below 600 nm have been selectively coupled to intracellular proteins using one of the methods described above, the coupling of fluorophores to proteins with excitation and emission maxima above 600 nm remains problematic due to the membrane impermeability of such fluorophores and usually requires the introduction of the fluorophore into the cell through invasive methods such as microinjection (Keppler, A.; Arrivoli, C.; Sironi, L.; Ellenberg, J. Biotechniques 2006, 41, 167), bead-loading (Maurel, D.; Banala, S.; Laroche, T.; Johnsson, K. ACS Chem Biol 2010, 5, 507) or electroporation (Jones, S. A.; Shim, S. H.; He, J.; Zhuang, X. Nat Methods 2011, 8, 499). 
       BRIEF SUMMARY OF THE INVENTION 
       [0006]    A new class of Si-rhodamine derivatives is described (sometimes referred to hereinafter as Si- and Ge-carboxy-rhodamines, respectively) that (i) absorb and admit light at wave-lengths above 600 nm; (ii) possess high photostability; (iii) have high extinction coefficients and high quantum yields; (iv) can be derivatizated with different molecules; and (v) are membrane-permeable and show minimal background binding to biomolecules and biomolecular structures. The general structure of the new Si- and Ge-carboxyrhodamine derivatives is exemplarily shown below: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0007]    An important feature of these fluorophores is the presence of a carboxyl group at the 2-position of the benzyl ring which dramatically increases membrane permeability and opens up various applications in biomolecular imaging. The carboxyl group permits the formation of a spirolactone, as outlined in more detail hereinafter. 
         [0008]    For the avoidance of doubt, it is to be noted that compounds of the invention may be differently charged, e.g. due to pH changes. Moreover, compounds of the invention may be provided in zwitterionic structure or may comprise counterions. None of the aformentioned derivates shah be deemed to be out of the scope of invention. 
         [0009]    As an example, the benzylguanine derivative of Si-carboxy-rhodarnine (BG-Si-carboxy-rhodamine) for labeling of SNAP-tag fusion proteins in living cells was prepared (SiR65O-6BG; of Section B.3 hereinbelow): 
         [0000]    
       
                 
         
             
             
         
       
     
         [0010]    BG-Si-carboxy-rhodamine possesses excellent membrane permeability and permits the specific labeling of SNAP-tag fusion proteins (the SNAP-tag technology as such is known in the art and suitable ready-to-use kits are commercially available from New England BioLabs, Inc.) in mammalian cells by simply incubating cells with BG-Si-carboxy-rhodamine, as demonstrated by fluorescence imaging. In contrast, the derivative with a methyl group at the 2-position of the benzyl ring (BG-Si-rhodamine) does not permit the specific labeling of SNAP-tag fusion proteins in living cells. This demonstrates the superior permeability and biocompatibility of the Si-carboxy-rhodamine derivatives. Importantly, the use of Si-carboxy-rhodamine derivatives is not limited to the labeling of SNAP-tag fusion proteins but can also be employed for the labeling of Halo-tag and Cup-tag fusion proteins. This demonstrates that Si-carboxy-rhodamine represent a unique μlatform for the development of fluorescent probes for biomolecular imaging, in which the Si-carboxy-rhodamine cores structure is derivatized with: (i) reactive groups such as activated esters, (ii) ligands that specifically bind to other (bio)molecules in vitro or in vivo or (iii) molecules that can control the fluorescence properties of the fluorophore. An example for molecules that can control the fluorescence properties of the fluorophore are calcium indicators or substrates of enzymes. 
     
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         [0011]      FIG. 1 : Comparative fluorescent intensity studies of two prior art dyes and a compound according to the invention; 
           [0012]      FIG. 2 : Labeling of SNAP fusions with compounds according to the invention in living cells; 
           [0013]      FIG. 3 : Superresolution microscopy application of a compound according to the invention. 
       
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0014]    In general terms, the invention pertains to a compound of formula 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein:
       Y is chosen from the group consisting of Si, Ge and Sn;   R 1 , R 2 , R 3 , R 4 , R 5 , R 8 , R 20  and R 21  are independently any kind of substituents;   A is NR 8 R 9 , wherein R 8  and R 9  are independently any kind of substituents;   B is O or N + R 18 R 19 , wherein R 18  and R 19  are independently any kind of substituents;   C is NR 18 R 19 ;   R 10  of (I) has the substructure       
 
         [0000]    
       
                 
         
             
             
         
       
       
         
           
             wherein
           one of R 12  or R 16  or both is/are independently a carboxylic acid or a salt of a carboxylic acid; and   R 13 , R 14 , R 15  and optionally one of R 12  or R 16  are independently any kind of substituents;   
         
             either R 12  or R 16  of R 10  in combination with R of (II) forms a γ-spirolactone. 
           
         
       
     
         [0025]    Preferably, R 1 , R 2 , R 3 , R 4 , R 5  and R 6  are hydrogen; R 20  and R 21  preferably are C 1 -C 6  alkyl, either saturated or unsaturated, most preferably methyl. 
         [0000]    In further preferred embodiments of the compounds according to the invention,
       R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 20  and R 21  are independently hydrogen; C 1 -C 6  alkyl, C 1 -C 6  alkoxy, or aryl, wherein the alkyl, alkoxy, or aryl portions have one or more substituents chosen from the group consisting of F, Cl, Br, I;   A is NR 8 R 9 , wherein
           R 8  and R 9  are independently H, C 1 -C 6  alkyl, C 1 -C 6  carboxyalkyl, C 1 -C 6  sulfoalkyl, a salt of C 1 -C 6  carboxyalkyl, a salt of C 1 -C 6  sulfoalkyl, wherein each aforementioned alkyl is optionally substituted with F, amino, hydroxyl, a carboxylic acid, a salt of a carboxylic acid, or a carboxylic acid ester or a C 1 -C 6  alkyl; or   R 8  in combination with R 9  forms a five- or six-membered hetero-cyclic substructure chosen from the group consisting of piperidines, morpholines, pyrrolidines or piperazines, wherein each of the aforementioned heterocyclic substructures is optionally substituted by methyl, F, a carboxylic acid, a salt of a carboxylic acid or a carboxylic acid ester or a C 1 -C 6  alkyl; or   one of R 8  or R 9  in combination with R 2  forms a five- or six-membered ring substructure, saturated or unsaturated, which is optionally substituted by one or more C 1 -C 6  alkyl or CH 2 SO 3 X, wherein X is H or a counterion; and/or one of R 6  or R 9  in combination with R 3  forms a five- or six-membered ring substructure, saturated or unsaturated, which is optionally substituted by one or more C 1 -C 6  alkyl, F or CH 2 SO 3 X, wherein X is H or a counterion;   
           B is O or N + R 18 R 19 , wherein
           R 18  and R 19  are independently H, C 1 -C 6  alkyl, C1-C 6  carboxyalkyl, C 1 -C 6  sulfoalkyl, a salt of C 1 -C 6  carboxyalkyl, a salt of C 1 -C 6  sulfoalkyl, wherein each aforementioned alkyl is optionally substituted with F, amino, hydroxyl, a carboxylic acid, a salt of a carboxylic acid, or a carboxylic acid ester or a C 1 -C 6  alkyl; or   R 18  in combination with R 19  forms a five- or six-membered hetercyclic substructure chosen from the group consisting of piperidines, morpholines, pyrrolidines or piperazines, wherein each of the aforementioned heterocyclic substructures is optionally substituted by F, methyl, a carboxylic acid, a salt of a carboxylic acid or a carboxylic acid ester or a C 1 -C 8  alkyl; or   one of R 18  or R 19  in combination with R 5  forms a five- or six-membered ring substructure, saturated or unsaturated, which is optionally substituted by one or more C 1 -C 6  alkyl or CH 2 SO 3 X, wherein X is H or a counterion; and/or one of R 18  or R 19  in combination with R 4  forms a five- or six-membered ring substructure, saturated or unsaturated, which is optionally substituted by one or more C 1 -C 6  alkyl, F or CH 2 SO 3 X, wherein X is H or a counterion;   
           C is NR 18 W 9 ;   in substructure (III)
           one of R 12  or R 16  or both is/are independently a carboxylic acid or a salt of a carboxylic acid, or suifonic acid or a salt of a sulfonic acid; and   R 13 , R 14  and R 15  are independently
               H, F, Cl, Br, 1, SO 3 X, a carboxylic acid, a salt of a carboxylic acid, an ester of a carboxylic acid, an amide, CN, nitro, hydroxyl, azido, amino, hydrazine; or   C 1 -C 19  alkyl, C 1 -C is  alkoxy, C 1  C, s  alkylthio, C 1 -C 18  alkanoylamino, C 1 -C 18  alkylaminocarbonyl, C 2 -C 36  dialkylaminocarbonyl, C 1 -C 18  alkyloxycarbonyl, C 8 -C 18  arylcarboxamido, wherein the alkyl portion(s) of each of the aforementioned is/are
                   optionally substituted one or more times with F, Cl, Br, I, hydroxy, a carboxylic acid, a salt of a carboxylic acid, a carboxylic ester of a C 1 -C 8  alcohol, —SO 3 X, amino, C 1 -C 5  alkylamino, C 2 -C 12  dialkylarnino, C 1 -C 8  alkoxy; and/or   optionally comprise one or more alkenyl and/or alkynyl moieties; or   
                   at least one pair of adjacent substituents R 13  and R 14 , or R 14  and R 15 , when taken in combination, forms a fused six-membered aromatic substructure that is optionally further substituted by a carboxylic acid or a salt of a carboxylic acid;   
               
           either R 12  or R 16  of R 10  in combination with R 11  of (II) forms a γ-spirolactone.       
 
         [0045]    In accordance with further preferred embodiments, R 5  or R 9  in combination with R 2  forms substructure 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein
       R′ denotes the respective one of R 0  and R 9  which is not incorporated into the ring of substructure (IV);   R 22  and R 23  are independently hydrogen; C 1 -C 6  alkyl, branched or linear; C 1 -C 6  substituted alkyl, branched or linear, in particular F substituted C 1 -C 6  alkyl, branched or linear; C 1 -C 6  alkenyl, branched or linear; C 1 -C 6  substituted alkenyl, branched or linear; C 1 -C 6  alkynyl, branched or linear; C 1 -C 6  substituted alkynyl, branched or linear; aryl; substituted aryl; hydroxyl; halogen; alkoxyl; carboxyl substituents;   D represents O; S; Se; Te; or preferably C(R 24 )(R 25 )—, with R 24  and R 25  being independently chosen from the group consisting of hydrogen; C 1 -C 6  alkyl, branched or linear; C 1 -C 6  substituted alkyl, branched or linear, in particular F substituted C 1 -C 6  alkyl, branched or linear; C 1 -C 6  alkenyl, branched or linear; C 1 -C 6  substituted alkenyl, branched or linear; C 1 -C 6  alkynyl, branched or linear; C 1 -C 6  substituted alkynyl, branched or linear; aryl; substituted awl; hydroxyl; halogen; alkoxyl; carboxyl substituents.       
 
         [0049]    Similarly to what has been outlined above with respect to substructure (IV), further preferred embodiments of the invention are compounds, wherein R 16  or R 19  in combination with R 5  forms substructure 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    wherein
       R″ denotes the respective one of R 18  and R 19  which is not incorporated into the ring of substructure (V);   R 26  and R 27  are independently hydrogen; C 1 -C 6  alkyl, branched or linear; C 1 -C 6  substituted alkyl, branched or linear, in particular F substituted C 1 -C 3  alkyl, branched or linear; C 1 -C 6  alkenyl, branched or linear; C r C 6  substituted alkenyl, branched or linear; C 1 -C 6  alkynyl, branched or linear; C 1 -C 5  substituted alkynyl, branched or linear; aryl; substituted aryl; hydroxyl; halogen; alkoxyl; carboxyl radicals;   E represents O; S; Se; Te; or preferably —C(R 28 )(R 29 )—, with R 28  and R 23  being independently chosen from the group consisting of hydrogen; C 1 -C 6  alkyl, branched or linear; C 1 -C 6  substituted alkyl, branched or linear, in particular F substituted C 1 -C 5  alkyl, branched or linear; C 1 -C 6  alkenyl, branched or linear; C 1 -C 6  substituted alkenyl, branched or linear; C 1 -C 5  alkynyl, branched or linear; C 1 -C 6  substituted alkynyl, branched or linear; aryl; substituted aryl; hydroxyl; halogen; alkoxyl; carboxyl.       
 
         [0053]    More specifically, currently preferred embodiments of the invention are the following cornpounds: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    with R 13 , R 14 , R 15  and R 16  as defined hereinbefore; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    with R 13 , R 14 , R 15  and R 16  as defined hereinbefore; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    with
         13 , R 14 , R 15  and R 16  as defined hereinbefore; and   R 2 , R 3 , R 4  and R 5  being independently H, F, Cl, Br;
 
and alternative protonation stages of (VI), (VII) and (VIII), comprising negatively or positively charged counterions,
       
 
         [0056]    Even more specifically, currently preferred embodiments of the invention are: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    and alternative protonation stages of (IX) and (X), comprising negatively or positively charged counterions. 
         [0057]    A further embodiment of the invention pertains to compounds as outlined hereinbefore, wherein at least one of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 8 , R 9 , R 10 , R 11 , R 13 , R 14 , R 15 , R 17 , R 18 , R 19 , R 20 , R 21 , R 22 , R 23 , R 24 , R 25 , R 26 , R 27 , preferably at least one of R 13 , R 14  and R 15 , is —L—R*, and wherein
       each L independently is a covalent linkage, each or some of the L being the same or different;   each R* independently is chosen from the group consisting of acrylamide; an activated ester of a carboxylic acid; a hydroxyl; an anhydride of a carboxylic acid; an aldehyde; an alkyl halide; a sulfonate; an amine; an anhydride; an aniline; an aryl halide; an azide; an alkyne; a boronate; a carboxylic acid; a carbodiimide; a diazoalkane; an epoxide; a glycol; a haloacetamide; a halotriazine; a hydrazine; a hydroxylamine; an imido ester; an isocyanate; an isothiocyanate; a ketone; a maleimide; a phosporamidite; a sulfonyl halide; a thiol; an alkine; a phosphine; a sulfonyl ester —CH 2 OSO 2 R, wherein R is C 6 H 4 CH 3  (tosyl), CH 3  (mesyl), CF 3  (triflate) or CF 2 CF 3  (nonaflate).       
 
         [0060]    Due to the reactive groups being introduced by the —L—R* moiety, the compounds of the invention can thereby be modified such as to allow for reactivity towards certain targets. Introduction of such reactive groups into the compounds of the invention can be accomplished by routine procedures known to the person of skill in the art. 
         [0061]    More specifically, currently preferred embodiments of compounds possessing such —L—R* moieties as outlined hereinbefore are: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone and with n=1-11; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone and with n=1-11;
 
and alternative protonation stages of (XI), (XII), (XIII) and (XIV), comprising negatively or positively charged counterions,
 
         [0062]    Yet a further embodiment of the invention pertains to compounds as outlined hereinbefore, wherein at least one of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 8 , R 9 , R 10 , R 11 , R 13 , R 14 , R 15 , R 17 , R 18 , R 19 , R 20 , R 21 , R 22 , R 23 , R 24 , R 25 , R 26 , R 27 , preferably at least one of R 13 , R 14  and R 15  is —L′—S, and wherein
       each L′ independently is a covalent linkage, each or some the same or different;   each S independently is an amino acid; a peptide; a protein; a monosaccharide; a disaccharide; a polysaccharide; an ion-complexing group, preferably a calcium-complexing group; a lanthanide-complexing group; a nickel-complexing group; a cobalt-complexing group; ethylenediamine tetraacetic acid; nitrilotriacetic acid; a nucleotide; a substrate of an enzyme; an inhibitor of an enzyme, preferably an irreversible inhibitor of an enzyme forming a covalent bond with an enzyme; an agonist of a receptor; a ligand that binds with a KD of at least 10 μM to a nucleic acid; a ligand that binds with a KD of at least 10  μM  to a protein; a substrate of SNAP-tag; a substrate of CLIP-tag; a substrate of Halo-tag, a ligand binding to dihydrofolate reductase; methotrexate; trimethoprim; a substrate of biotin ligase; a substrate of phosphopantetheine transferase; a substrate of lipoic acid ligase; biotin; a ligand binding to streptavidin, avidin or neutravidin; a cofactor of an enzyme; a hormone; a toxin; a fluorophore; a nucleic acid polymer; a hapten; an antigen; a drug; a lipid; a lipid assembly; a non-biological organic polymer; a polymeric microparticle; an animal cell a plant cell; a bacterium, a yeast; a virus; a protist.       
 
         [0065]    More specifically, currently preferred embodiments of compounds possessing such —L′—S moieties as outlined hereinbefore are: 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone, 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone; 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    or its corresponding spirolactone;
 
and alternative protonation stages of (XV), (XVI), (XVII), (XVIII), (XIX), (XX), (XXI), (XXII), (XXIII), (XXIV), comprising negatively or positively charged counterions.
 
         [0066]    It is being understood that currently preferably L and L′ is/are independently a single covalent bond, or L and L′ is/are a covalent linkage having 1-24 non-hydrogen atoms selected from the group consisting of C, N, O, P and S and is composed of any combination of single, double, triple or aromatic carbon-carbon bonds, carbon-nitrogen bonds, nitrogen-nitrogen bonds, carbon-oxygen bonds, carbon-sulfur bonds, phosphorous-oxygen bonds and phosphorous-nitrogen bonds. 
         [0067]    A further aspect of the invention pertains to the use of the aforementioned compounds comprising a L-R* moiety as outlined hereinbefore, in a reaction with a substrate molecule that binds or can preferably be enzymatically coupled to a specific target, in particular a protein or peptide, resulting in a compound comprising a —L′—S moiety as outlined hereinbefore, wherein the reaction occurs between the substrate molecule and the compound comprising a —L—R* moiety at least at one of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 13 , R 14  and R 15 , preferably at least one of R 13 , R 14  and R 15 , thereby establishing a binding moiety towards a specific target at least at one of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 13 , R 14  and R 15 , preferably at least one of R 13 , R 14  and R 15 . 
         [0068]    More specifically, in the use as outlined above, the specific target, in particular the protein or peptide on the one hand and the binding moieties on the other hand are chosen from the group consisting of SNAP-tag and benzylguanine; CLIP-tag and benzylcytosine; HALO-tag and 1° chloride; dihydrofolate reductase; trimethoprim; kinase and kinase inhibitor; DNA polymerase and its substrate(s). 
         [0069]    In accordance with currently preferred embodiments of the use as outlined above, the substrate can be enzymatically coupled to the target by an enzyme chosen from the group consisting of phosphopantetheine transferase, biotin ligase, liopoic acid ligase; DNA polymerase; DNA methyltransferase. 
         [0070]    Yet a further aspect of the invention pertains to a method of providing a binding agent for a specific target, in particular a protein, peptide or nucleic acid, characterized in that a compound comprising an —L—R* moiety as outlined hereinbefore at least at one of R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 13 , R 14  and R 15 , preferably at least one of R 13 , R 14  and R 15  is reacted with a substrate molecule that binds or can preferably be enzymatically bound to said target. 
         [0071]    Another aspect of the present invention is a kit-of-parts, comprising
       i) a compound as outlined hereinbefore;   ii) optionally, a second compound that is able to bind to a specific target, in particular a protein or peptide, and which second compound is able to react with a compound comprising a —L—R moiety as outlined hereinbfore at least at one of R 1 , R 2 , R 3 , and R 4 , preferably at least one of R 2  and R3;   iii) optionally, an activating agent to allow for the reaction of either i) or the reaction product of i) and ii) with the specific target to occur;   iv) optionally, instructions for use of the kit-of-parts in accordance with the various methods and uses outlined herein.       
 
         [0076]    In especially preferred embodiments, the compound i) is able to bind to a specific target, in particular by means of a L-S moiety as outlined hereinbefore. 
         [0077]    It is being understood that the compounds as outlined herein and the kit-of-parts is especially useful for the labelling of proteins or nucleic acids in vitro, in living cells or in living organisms. This will be readily apparent for the person of skill in the art, especially in view of the experimental details and applications outlined hereinafter. 
         [0078]    In particular, the compounds and the kit-of-parts as outlined hereinbefore will prove useful in fluorescence spectroscopy; fluorescence microscopy; fluorescence imaging; stochastic optical reconstruction microscopy (STORM); direct STORM (dSTORM); ground state depletion microscopy followed by individual molecule return (GSDIM); ground state depletion (GSD) microscopy; single-molecule spectroscopy; Forster resonance energy transfer (FRET) applications, in particular time-resolved; fluorescence correlation spectroscopy; fluorescence anisotropy spectroscopy; correlative fluorescence electron microscopy; fluorescence activated cell sorting; oxygen, fluoride or glycerol sensing in vitro, in living cells or living organisms. 
       Experimental Details 
       [0079]    The invention will be described below by means of embodiments and experiments; it is to be understood that this is not intended to limit the subject-matter of the invention in any way. 
       A. Synthesis 
       [0080]    All chemical reagents and dry solvents for synthesis were purchased from commercial suppliers (Sigma-Aldrich, Fluka, Acros) and were used without further purification or distillation. The composition of mixed solvents is given by the volume ratio (v/v). Thin layer chromatography (TLC) was performed on TLC-aluminum sheets (Silica gel 60 F 254 ). Flash column chromatography was performed with Merck silica gel (230-400 mesh).  1 H and  13 C nuclear magnetic resonance (NMR) spectra were recorded on a Bruker DPX 400 (400 MHz for  1 H, 100 MHz for  13 C, respectively) or Bruker 500 (500 MHz for  1 H, 125 MHz for  13 C, respectively), with chemical shifts (δ) reported in ppm relative to the solvent residual signals of CDCl 3  (7.16 ppm for  1 H, 77.16 ppm for  13 C), CD 3 OD (3.31 ppm for  1 H, 49.00 ppm for  13 C), DMSO-d 6  (2.50 ppm for  1 H, 39.52 ppm for  13 C), acetone-d 6  (2.05 ppm for  1 H, 29.84 ppm for  13 C), and coupling constants reported in Hz. High resolution mass spectra (HRMS) were measured on a Micromass Q-TOf Ultima spectrometer with electron spray ionization (ESI). Reversed phase analytical HPLC was run on a Waters 2790 separation module and products were detected at 280 nm using a 2487 dual A absorption detector. The standard gradient that was used for the purifications was: starting at water including 0.1% TFA for 2 minutes and raising to 100% acetonitrile within 17 minutes. A 3,9×300 mm Prep Nova-Pak HR C18 6 μm column from Waters was used to determine the purity of the products. Preparative HPLC was performed on a Waters 600 controller and with a Waters 2487 dual λ absorption detector using a SunFire™ Prep C18 OBD™ 5 μm 19×150 mm Column. 
         [0081]    The general outline of the synthesis is as follows (for more details cf Sections A.1 to A.12, below): 
         [0000]    
       
                 
         
             
             
         
       
     
         [0000]    A.1 4,4′-methylenebis(3-bromo-N,N-dimethylaniline): (1) 
         [0000]    
       
                 
         
             
             
         
       
     
         [0082]    3-Bromo-N,N-dimethylaniline (5.00 g, 25 mmol, 2 eq.) was dissolved in 37% formaldehyde solution (5 ml) and acetic acid (40 ml), and stirred at 60° C. for 30 minutes. After cooling, acetic acid was evaporated, then saturated NaHCO 3  aqueous solution was added carefully. The aqueous phase was extracted with ethyl acetate twice, and the combined organic phase was washed with water and brine, dried over Na 2 SO 4 , then filtered and evaporated. The resulting residue was purified by silica gel column chromatography (n-hexane/EtOAc=90/10) to obtain 4,4′-methylenebis(3-bromo-N,N-dimethylaniline) 1 as a white solid. (3.24 g, 63%) 
         [0083]      s1 H NMR (400 MHz, CDCl 3 ) δ 6.94 (d, 2 H, J=2.7 Hz), 6.85 (d, 2 H, J=8.6 Hz), 6.59 (dd, 2 H, J=8.6, 2.6 Hz), 4.00 (s, 2 H), 2.92 (s, 12 H). 
         [0084]      13 C NMR (100 MHz, CDCl 3 ) δ 150.2, 130.9, 127.2, 125.7, 116.4, 112.0, 40.7, 40.0. HRMS (ESI): m/z calc. for C 17 H 20 Br 2 N 2  411.0071, 413.0052; found 411.0092 (5.21 ppm), 413.0056 (0.97 ppm) [M+H] 30    
         [0000]    A.2 3,7-Bis(dimethylamino)-5,5-dimethyldibenzo[b,e]silin-10 (5H)-one: (3) 
         [0000]    
       
                 
         
             
             
         
       
     
         [0085]    4,4′-Methylenebis(3-bromo-N,N-dirnethylaniline 1 (2.00 g, 4.85 mmol, 1 eq.) was dissolved in dry THF (200 ml) and stirred at −78° C. on the CO 2 (s)/acetone bath. sec-BuLi (1.4 mol/l solution in n-hexane, 10 ml, 14.0 mmol, 3 eq.) was slowly added for 30 minutes to the solution and stirred for further 2 hr at the same temperature. SiMe 2 Cl 2  (1 ml, 8.22 mmol, 1.8 eq.) was added to the reaction mixture and stirred at room temperature for 2 hr. 1 N HCl aqueous solution was added carefully to neutralize the solution, and THF was evaporated. The resulting aqueous solution was extracted with EtOAc, and the organic phase was washed with saturated NaHCO 3  aqueous solution, water and brine, dried over Na 2 SO 4 , filtered and evaporated to obtain the crude including N 3 ,N 3 ,N 7 ,N 7 ,5,5-hexamethyl-5,10-dihydrodibenzo[b,e]siline-3,7-diamine 2, which was used for the next reaction immediately due to its high sensitivity toward oxygen. The resulting crude including 2 was dissolved in acetone (30 ml) and stirred at −15° C. on the crashed ice/NaCl(s) bath. MnC 4  powder was added portionwise (300 mg×6) for 30 minutes, and stirring was continued for further 2 hr at the same temperature. The purple suspension was filtered through a Celite pad, and the yellow filterate was evaporated. The resulting residue was purified by silica gel column chromatography (n-hexane/CH 2 Cl 2 =20/80) to obtain 3,7-Bis(dimethylamino)-5,5-dimethyldibenzo[b,e]silin-10(5H)-one as a yellow solid. (689 mg, 41%) 
         [0086]      1 H NMR (400 MHz, CDCl 3 ) δ 8.44 (d, 2 H, J=8.9 Hz), 6.87 (dd 2 H, J=9.0 Hz, 2.4 Hz), 6.83 (d, 2 H, J=2.4 Hz), 3.12 (s, 12 H), 0.51 (s, 6 H). 
         [0087]      13 C NMR (100 MHz CDCl 3 ) δ 185.4, 151.5, 140.6, 131.7, 129.7, 114.4, 113.2, 40.2, −0.8. 
         [0088]    HRMS (ESI): m/z calc. for C 19 H 24 N 2 OSi 325.1736; found 325.1730 (−1.85 ppm), [M+H] +   
         [0000]    A.3 4-bromo-N 1 ,N 3 -bis(1-hydroxy-2-methylpropan-2-yl)isophthalamide: (4a) 
         [0000]    
       
                 
         
             
             
         
       
     
         [0089]    2-Bromoisophthalic acid (1.50 mg, 6.12 mmol, 1 eq.) was suspended into SOCl 2  (10 ml) in the presence of DMF (1 drop), and the solution was refluxed for 2 hr. After cooling to room temperature and evaporating SOCl 2  and dried in vacuo, the resulting compound was dissolved in CH 2 Cl 2  (30 ml), and was dropped into a solution of 2-amino-2-methylpropan-1-ol (1.10 g, 12.3 mmol, 2 eq.) and DIEA (2 ml) in CH7C1 2  (30 ml), and the solution stirred at room temperature overnight. Saturated NaHCO 3  aqueous solution was added to the reaction mixture and extracted with EtOAc (×3), and the combined organic phase was washed with water and brine, dried over Na 2 SO 4 , filtered and evaporated to obtain 4-bromo-N 1 ,N 3 -bis(1-hydroxy-2-methylpropan-2-yl)isophthalamide 4a as a white solid. (2.09 g, 97%) 
         [0090]      1 H NMR (400 MHz, DMSO-d 6 ) δ 7.87 (s, 1 H), 7.78 (d, 1 H, J=2.1 Hz), 7.71 (m, 3 H), 4.88 (t, 1 H, J=6.0 Hz), 4.82 (t, 1 H, J=6.1 Hz), 3.51 (d, 2 H,=5.9 Hz), 3.50 (d, 2 H, J=5.9 Hz), 1.31 (s, 6 H), 1.30 (s, 6H). 
         [0091]      13 C NMR (100 MHz, DMSO-d 6 ) δ 167.2, 165.5, 140.0, 135.0, 132.7, 129.6, 127.8, 122.1, 67.9, 67.6, 55.8, 55.7, 24.0, 23.9. 
         [0092]    HRMS (ESI): m/z calc. for C 16 H 23 BrN 2 O 4  387.0919; found 387.0911 (−2.07 ppm), [M+H] +   
         [0000]    A.4 2,2° -(4-bromo-1,3-phenylene)bis(4,4-dimethyl-4,5-dihydrooxazole): (5a) 
         [0000]    
       
                 
         
             
             
         
       
     
         [0093]    2-Bromo-N 1 ,N 3 -bis(1-hydroxy-2-methylpropan-2-yl)isophthalamide (900 mg, 2.32 mmol) was dissolved in SOCl 2  (5 mi) and stirred at room temperature for 1 hr. After evaporating SOCl 2 , saturated NaHCO aqueous solution was added carefully to neutralize the solution. The resulting water phase was extract with EtOAc (×2), and the combined organic phase was washed with water and brine, dried over Na 2 SO 4 , filtered and evaporated. The resulting residue was purified by silica gel column chromatography (CH 2 Cl 2 /MeOH=95/5) to obtain 2,2′(4-bromo-1,3-phenylene)bis(4,4-dimethyl-4,5-dihydrooxazole) 5a as a colorless liquid. (772 mg, 94%) 
         [0094]      1 H NMR (400 MHz, CDCl 3 ) δ 8.21 (d, 1 H, J=2.1 Hz), 7.82 (dd, 1 H, J=8.4, 2.1 Hz), 7.67 (d, 1 H, J=8.4 Hz), 4.14 (s, 2 H), 4.12 (s, 2 H), 1.42 (s, 6 H), 1.38 (s, 6 H).  13 C NMR (100 MHz, CDCl 3 ) δ 160.9, 160.7, 133.7, 130.9, 130.8, 130.6, 127.3, 125.0, 79.5, 79.4, 68,4, 67.9, 28.4, 28.3. 
         [0095]    HRMS (ESI): m/z calc. for C 16 H 19 BrN 2 O 2  351.0708; found 351.0704 (−1.14 ppm), [M+H] +   
         [0000]    A.5 2-bromo-N 1 ,N 4 -bis(1-hydroxy-2-methylpropan-2-yl)terephthalamide (4b) 
         [0000]    
       
                 
         
             
             
         
       
     
         [0096]    2-Bromoterephthalic acid (750 mg, 3.06 mmol, 1 eq.) was suspended into SOCl 2  (5 ml) in the presence of DMF (1 drop), and the solution was refluxed for 3 hr. After cooling to room temperature, evaporating and dried in vacuo, the resulting compound was dissolved in CH 2 Cl 2  (10 ml), and was dropped into a solution of 2-amino-2-methylpropan-1-ol (750 mg, 8.41 mmol, 2.7 eq.) and DIEA (1.5 ml) in CH 2 Cl 2  (10 ml), and the solution was stirred at room temperature overnight. Saturated NaHCO 3  aqueous solution was added to the reaction mixture and extracted with EtOAc (×3), and combined organic phase was washed with water and brine, dried over Na 2 SO 4 , filtered and evaporated to obtain 2-bromo-N 1 ,N 4 -bis(1-hydroxy-2-methylpropan-2-yl)terephthalamide 4b as a white solid. (922 mg, 78%) 
         [0097]      1 H NMR (400 MHz, DMSO-d 5 ) δ 8.04 (d, 1 H, J=1.5 Hz), 7.86 (bs, 1 H), 7.82 (dd, 1 H, J=7.9, 1.7 Hz), 7.72 (bs, 1 H), 7.43 (d, 1 H, J=7.9 Hz), 4.87 (1, 1 H, J=6.1 Hz), 4.82 (t, 1 H, J=6,1 Hz), 3.51 (d, 2 H, J=6.2 Hz), 3.50 (d, 2 H, J=6.2 Hz), 1,31 (s, 6 H), 1.30 (s, 6H). 
         [0098]      13 C NMR (100 MHz, DMSO-d 6 ) δ 167.2, 164.9, 142.3, 137.7, 131.6, 128.8, 126.9, 119.1, 67.8, 67.5, 55.8, 24.0, 23.9. 
         [0099]    HRMS (ESI): m/z calc. for C 16 H 23 BrN 2 O 4  387.0919; found 389.0915 (−1.03 ppm), [M+H] +   
         [0000]    A.6 2,2′42-bromo-1,4-phenylene)bis(4,4-dimethyl-4, -dihydrooxazole): (5b) 
         [0000]    
       
                 
         
             
             
         
       
     
         [0100]    2-Bromo-N 1 ,N 4 -bis(1-hydroxy-2-methylpropan-2-yl)terephthalamide (880 mg, 2.27 mmol) was dissolved in SOCl 2  (4 ml) and stirred at room temperature for 90 minutes. After evaporating SOCl 2 , saturated NaHCO 3  aqueous solution was added carefully to neutralize the solution. The resulting water phase was extract with EtOAc (×2), and the combined organic phase was washed with water and brine, dried over Na 2 SO 4 , filtered and evaporated. The resulting residue was purified by silica gel column chromatography (EtOAc/C 1 --1 2 C1 2 =50/50 to 100/0) to obtain 2,2′-(2-bromo-1,4-phenylene)bis(4,4-dimethyl-4,5-dihydrooxazole) 5b as a white solid. (578 mg, 73%)  1 H NMR (400 MHz, DMSO-d 6 ) δ 8,08 (d, 1 H, J=1.5 Hz), 7.91 (dd, 1 H, J=8.1, 1.7 Hz), 7.77 (d, 1 H, J=8.0 Hz), 4.16 (s, 2 H), 4.13 (s, 2 H), 1.32 (s, 6 H), 1.30 (s, 61−1). 
         [0101]      13 C NMR (100 MHz, DMSO-d 5 ) δ 160.0, 159.3, 132.7, 132.6, 132.1, 131.3, 127.3, 121.4, 79.3, 79.1, 68.7, 68.3, 28.6, 28.4. 
         [0102]    HRMS (ESI): m/z calc. for C 16 H 19 BrN 2 O 2  351.0708; found 351.0719 (3.13 ppm), [M+H] +   
         [0000]    A.7 tert-butyl 4-bromo-3-methylbenzoate: 5c 
         [0000]    
       
                 
         
             
             
         
       
     
         [0103]    4-Bromo-3-methylbenzoic acid (740 mg, 3.44 mmol, 1 eq.). (Boc) 2 O (1.92 g, 8.78 mmol, 2.5 eq.) and DMAP (93 mg, 0.764 mmol, 1 eq.) were dissolved in dry THF (10 ml) and refluxed overnight. After cooling to room temperature and evaporating the solvent, the residue was dissolved in Et 2 O and washed with saturated NaHCO 3  aqueous solution, water and brine, dried over Na 2 SO 4 , filtered and evaporated. The resulting crude was purified by silica gel column chromatography (n-hexane/EtOAc=95/5) to obtain tert-butyl 4-bromo-3-methylbenzoate as a pale yellow liquid. (647 mg, 87%) 
         [0104]      1 H NMR (400 MHz, CDCl 3 ) δ7.83 (d, 1 H, J=1.7 Hz), 7.64 (dd, 1 H, J=8.9, 1.6 Hz), 7.56 (d, 1 H, J=8.8 Hz), 2.43 (s, 3 H), 1.59 (s, 9 H). 
         [0105]      13 C NMR (100 MHz, CDCl 3 ) δ 165.3, 138.0, 132.3 1.6, 131.1, 129.8, 128.2, 81.4, 28.2, 22.9. 
         [0000]    A.8 tert-butyl 3-bromo-4-methylbenzoate: 5d 
         [0000]    
       
                 
         
             
             
         
       
     
         [0106]    3-Bromo-4-methylbenzoic acid (1.40 g, 6.51 mmol, 1 eq.), (Boc) 2 O (3.62 g, 16.6 mmol, 2.5 eq.) and DMAP (180 mg, 1.47 mmol, 0.2 eq.) were dissolved in dry THF (20 ml) and refluxed overnight. After cooling to room temperature and evaporating the solvent, the residue was dissolved in Et 2 O and washed with saturated NaHCO 3  aqueous solution, water and brine, dried over Na 2 SO 4 , filtered and evaporated. The resulting crude was purified by silica gel column chromatography (n-hexane/EtOAc=95/5) to obtain tert-butyl 3-bromo-4-methylbenzoate 5d as a colorless liquid. (951 mg, 54%) 
         [0107]      1 H NMR (400 MHz, CDCl 3 ) δ 8.15 (d, 1 H, J=1.6 Hz), 7.83 (dd, 1 H, J=7,9, 1.6 Hz), 7.29 (d, 1 H, J=7.9 Hz), 2.46 (s, 3 H), 1.61 (s, 9 H). 
         [0108]      13 C NMR (100 MHz, CDCl 3 ) δ 164.5, 142.7, 133.3, 131.4, 130.5, 128.2, 124.6, 81.4, 28.2, 23.2. 
       A.9 SiR650-5COOH 
       [0109]    
       
                 
         
             
             
         
       
     
         [0110]    In an argon-flushed flask fitted with a septum cap, 5a (450 mg, 1.28 mmol, 2 eq.) was dissolved in dry THF (5 ml) and cooled at −78° C. on the CO 2 (s)/acetone bath. tert-BuLi (800 μl, 1.28 mmol, 2 eq.) was dropped slowly and the solution was stirred at the same temperature for 1 hr. Compound 3 (188 mg, 0.581 mmol, 1 eq.) in dry THF (10 ml) was dropped via syringe at −78° C., and the solution was warmed to room temperature and stirred for 2 hr. Acetic acid (1 ml) was added to the reaction mixture on ice, the resulting intense blue solution was evaporated and lyophilized to obtain compound 6a as a blue solid, which was used for the next reaction without further purification. Compound 6a was dissolved in 6N HCl aq. (8 ml) and stirred at 80° C. overnight. After cooling to room temperature, the solution was added to saturated NaHCO 3  aqueous solution to adjust the pH to 1-2, and extracted with CH 2 Cl 2  (×3), and the combined organic phase was washed with 0.1 N HCl (×2) and brine, dried over Na 2 SO 4 , filtered and evaporated. The resulting crude was purified with silica gel column chromatography (CH 2 Cl 2 /MeOH 90/10) to obtain SiR650-6COOH as a blue solid. (89.0 mg, 35%) 
         [0111]      1 H NMR (400 MHz, CD 3 OD) δ 8.53 (s, 1 H), 8.36 (d, 1 H, J=7.8 Hz), 7.33 (d, 1 H, J=8.0 Hz), 7.06 (d, 2 H, J=2.6 Hz), 6.73 (d, J=9.0 Hz, 2 H), 6.66 (dd, J=9.0 Hz, 3.0 Hz, 2 H), 2.98 (s, 12 H), 0.66 (s, 3 H), 0.57 (s, 3 H), 
         [0112]      13 C NMR (100 MHz, CD 3 OD) δ 172.0, 171.4, 159.4, 156.4, 149.7, 136.5, 135.1, 131.3, 127.8, 126.3, 125.9, 123.8, 116.5, 113.4, 39.1, −1.0, −2.8, 
         [0113]    HRMS (ESI): m/z talc, for C 27 H 29 N 2 O 4 Si 473.1897; found 473.1892 (−1.06 ppm), [M+H] +   
       A.10 SiR650-6COOH 
       [0114]    
       
                 
         
             
             
         
       
     
         [0115]    In an argon-flushed flask fitted with a septum cap, 5b (150 mg, 0.427 mmol, 2 eq.) was dissolved in dry THE (5 ml) and cooled at −78° C. on the CO 2 (s)/acetone bath, tent-BuLi (300 μl, 0.481 mmol, 2 eq.) was dropped slowly and the solution was stirred at the same temperature for 1 hr. Compound 3 (69.0 mg, 0.214 mmol, 1 eq.) in dry THE (5 ml) was dropped via syringe at −78° C., and the solution was warmed to room temperature and stirred for 2 hr. Acetic acid (1 ml) was added to the reaction mixture on ice, the resulting intense blue solution was evaporated and lyophilized to obtain a blue solid, which was used for the next reaction without further purification. The intermediate was dissolved in 6N HCl aq. (12 mi) and stirred at 80° C. overnight. After cooling to room temperature, the solution was added to saturated NaHCO 3  aqueous solution (50 ml) to adjust the pH (1-2), and extracted with CH 2 Cl 2  (×3), and the combined organic phase was washed with 0.1 N HCl (×2) and brine, dried over Na 2 SO 4 , filtered and evaporated. The resulting crude was purified with silica gel column chromatography (CH 2 Cl 2 /MeOH=90/10) to obtain SiR650-6COOH as a blue solid. (79.2 mg, 86%) 
         [0116]      1 H NMR (400 MHz, CD 3 OD) δ 8.25 (dd, 1 H, J=8.0, 1.1 Hz), 8.05 (d, 1 H, J=8.0 Hz), 7.86 (d, 1 H, J=1.0 Hz), 7.08 (d, 2 H, J=2.8 Hz), 6.77 (d, 2 H, J=9.2 Hz), 6.68 (dd, 2 H, J=9.0, 2.9 Hz), 3.00 (s, 12 H), 0,68 (s, 3 H), 0.58 (s, 3 H). 
         [0117]      13 C NMR (125 MHz, CD 3 OD) δ 172.0, 171.6, 154.6, 149.8, 144.1, 136.7, 131.4, 129.6, 127.8, 127.3, 124.9, 124.4, 116.6, 113.5, 39.2, −0.9, −2.7, 
         [0118]    HRMS (ESI): m/z calc. for C 27 H 29 N 2 O 4 Si 473.1897; found 473.1898 (0.21 ppm), [M+H] +   
       A.11 SiRMe-5COOH (Comparative Example) 
       [0119]    
       
                 
         
             
             
         
       
     
         [0120]    In an argon-flushed flask fitted with a septum cap, 5 c (100 mg, 0.369 mmol, 4 eq.) was dissolved in dry THF (3 ml) and cooled at −78° C. on the CO 2 (s)/acetone bath. tert-BuLi (250 μl, 0.401 mmol, 4 eq.) was dropped slowly and stirred at the same temperature for 1 hr. Compound 3 (25.2 mg, 0.077 mmol, 1 eq.) in dry THF (2 ml) was dropped via syringe at −78° C., and warmed to room temperature and stirred overnight. 0.1 N HClaq. was added to the reaction mixture, and the resulting intense blue solution was basified with saturated NaHCO 3  aqueous solution, and extracted with CH 2 Cl 2  (×2). The combined organic phase was washed with brine, dried over Na 2 SO 4 , filtered and evaporated to obtain a blue solid, which was used for the next reaction without further purification. The intermediate was dissolved in 6N HCl aq. (8 ml) and MeCN (2 ml) and stirred at 40° C. for 1 hr. After cooling to room temperature, the solution was added to 0.1 N NaOHaq. to adjust the pH to 2−3, and extracted with CH 2 Cl 2  (×2), and the combined organic phase was washed with brine, dried over Na 2 SO 4 , filtered and evaporated. The crude was purified with silica gel column chromatography (CH2Cl 2 /MeOH=90/10) to obtain SiRMe-5COOH as a blue solid. (27.5 mg, 88%) 
         [0121]      1 H NMR (400 MHz, CD 3 OD) δ 8.09 (s, 1 H), 8.05 (dd, 1 H, J=7.9, 1.2 Hz), 7.40 (d, 2 H, J=2.8 Hz), 7.28 (d, 1 H, J=7.9 Hz), 7.05 (d, 2 H, J=97 Hz), 6.81 (dd, 2 H, J=9.7, 2.8 Hz), 3.37 (s, 12 H), 2.12 (s, 3 H), 0.64 (s, 3 H), 0.63 (s, 3 H). 
         [0000]      13 C NMR (100 MHz, CD 3 OD) δ167.8, 154.5, 148.1, 143.5, 140.5, 136.3, 131.2, 131.1, 129.2, 126.7, 126.6, 121.0, 114.0, 39.5, 18.0, −2.5, −2.7, 
         [0122]    HRMS (ESI): m/z talc, for C 27 H 31 N 2 O 2 Si 443.2155; found 443.2170 (3.40 ppm), [M+H] +   
       A.12 SiRMe-6COOH (Comparative Example) 
       [0123]    
       
                 
         
             
             
         
       
     
         [0124]    In an argon-flushed flask fitted with a septum cap, 5 d (100 mg, 0.369 mmol, 4 eq.) was dissolved in dry THF (3 ml) and cooled at −78° C. on the CO 2 (s)/acetone bath. tert-BuLi (250 pi, 0.401 mmol, 4 eq.) was dropped slowly and stirred at the same temperature for 1 hr. Compound 3 (24.0 mg, 0.074 mmol, 1 eq.) in dry THF (2 ml) was dropped via syringe at −78° C., and warmed to room temperature and stirred for 2 hr. 0.1N HClaq. was added to the reaction mixture, and the resulting intense blue solution was basified with saturated NaHCO 3  aqueous solution, and extracted with CH 2 Cl 2  (×3). The combined organic phase was washed with brine, dried over Na 2 SO4, filtered and evaporated to obtain a blue solid, which was used for the next reaction without further purification. The intermediate was dissolved in 6 N HCl aq. (8 ml) and MeCN (2 ml) and stirred at 40° C. for 1 hr. After cooling to room temperature, the solution was added to 0.1 N NaOHaq. to adjust the pH to 2−3, and extracted with CH 2 Cl 2  (×2), and the combined organic phase was washed with brine, dried over Na 2 SO 4 , filtered and evaporated. The crude was purified with silica gel column chromatography (CH 2 Cl 2 /MeOH=90/10) to obtain SiRMe-6COOH as a blue solid. (23 mg, 80%) 
         [0125]      1 H NMR (400 MHz, CD 3 OD) δ 8.12 (d, 1 H, J=7.3 Hz), 7.77 (s, 1 H), 7.49 (d, 1 H, J=7.6 Hz), 7.39 (s, 2 H), 7.07 (d, 2 H, J=9.5 Hz), 6.78 (d, 2 H, J=9.6 Hz), 3.37 (s, 12 H), 2.10 (s, 3 H), 0.65 (s, 3 H), 0.63 (s, 3 H). 
         [0126]      13 C NMR (100 MHz, DMSO-d 6 ) δ 167.3, 158.4, 158.1, 154.2, 147.7, 140.4, 138.6, 130.4, 130.0, 126.8, 122.0, 119.3, 116.3, 115.1, 41.0, 19.4, −0.6, −0.9. 
         [0127]    HRMS (ESI): miz calcd for C 27 H 31 N 2 O 2 Si 443.2155; found 443.2162 (1.58 ppm), [M+H] +   
       B. Synthesis of BG- or CP-Fluorophores 
       [0128]    O 6 —(4-Aminomethyl)benzylguanine (BG-NH2) and 4-(4-aminomethyl)benzyloxy-6-chloropyrimidin-2-amine (CP-NH2) were prepared according to the previously reported procedures (Keppler at al., Methods 2004, 32, 437; Srikun et al., C.J.J.Am,Chem.Soc. 2010, 132, 4455), respectively. 
         [0129]    SiR dyes (5.0 mg, 1 eq.), BG-NH 2  (6.5 mg 2.4 eq.) or CP-NH 2  (6.4 mg, 2.4 eq.), PyBOP (12.0 mg, 2.4 eq.) were dissolved in dry DMSO (500 μl) in the presence of DIEA (10 μl), and the solution was stirred at room temperature for 1-3 hr. The reaction mixture was purified by HPLC to obtain the desired BG- or CP-SiR conjugates. 
       B.1 SiR650-5BG (5.3 mg, 69%) 
       [0130]      1 H NMR (400 MHz, CD 3 OD) δ 9.40 (t, 1 H, J=5.9 Hz), 8.71 (d, 1 H, J=1.5 Hz), 8.35 (s, 1 H), 8.24 (dd, 1 H, J=8.0, 1.6 Hz), 7.59 (d, J=7.5 Hz, 2 H), 7.49 (d, J=7.5 Hz, 2 H), 7.43 (d, 1 H, J=8.0 Hz), 7.32 (d, 2 H, J=2.8 Hz), 6.96 (d, J=9.0 Hz, 2 H), 6.75 (dd, 2 H, J=9.5, 2.8 Hz), 5.69 (s, 2 H), 4.69 (s, 2 H), 3.28 (s, 12 H), 0.66 (s, 3 H), 0.60 (s, 3 H). 
         [0131]      13 C NMR (125 MHz, CD 3 OD) δ 170.7, 167.0, 160.3, 160.0, 157.6, 149.8, 138.7, 138.6, 136.6, 135.7, 135.6, 133.2, 130.7, 128.4, 127.8, 127.3, 126.8, 124.7, 124.0, 116.5, 113.4, 93.2, 67.3, 43.1, 39.1, −1.0, −2.8. 
         [0132]    HRMS (ESI): m/z calc. for C 40 H 41 N 6 O 4 Si 725.3020; found 725.3014 (−2.48 ppm), [M+H] +   
       B.2 SiR650-SSCP (6.1 mg, 80%) 
       [0133]      1 H NMR (400 MHz, CD 3 OD) δ 9.36 (t,1 H, J=5.6 Hz), 8.72 (m, 1 H), 8.23 (m, 1 H), 7.43 (m, 5 H), 7.32 (d, 2 H, J=2.8 Hz), 6.96 (d, 2 H, J=9.4 Hz), 6.76 (dd, 2 H, J=9,5, 2.9 Hz), 6.13 (s, 1 H), 5.38 (s, 2 H), 4.68 (d, 2 H, J=6.0 Hz), 3.34 (s, 12 H), 0.66 (s, 3 H), 0.60 (s, 3 H). 
         [0134]      13 C NMR (125 MHz, acetone-d 6 ) δ 170.9, 169.3, 165.0, 160.7, 157.4, 149.6, 139.5, 136.4, 135.9, 135.4, 133.4, 131.3, 128.5, 127.9, 127.8, 126.8, 124.7, 123.8, 116.6, 113.5, 95.3, 67.5, 43.0, 39.4, −0.5, −2.1. 
         [0135]    HRMS (ESI): m/z calc. for C 39 H 40 C1N 6 O 4 Si 719.2569; found 719.2567 (−0.28 ppm), [M+H] +   
       B.3 SIR650−6BG (7.0 mg, 91%) 
       [0136]      1 H NMR (400 MHz, DMSO-d 6 ) δ 9.36 (t, 1 H, J=5.0 Hz), 8.50 (s, 1 H), 8.14 (d, 1 H, J 7.6 Hz), 8.07 (d, 1 H, J=7.8 Hz), 7.71 (s, 1 H), 7.49 (d, 2 H, J=7.9 Hz), 7.34 (d, 2 H, J =7,8 Hz), 7.05 (s, 2 H), 6,66 (m, 4 H), 5.52 (s, 2 H), 4.45 (d, 2 H, J=5.2 Hz), 2.95 (s, 12 H), 0.63 (m, 3 H), 0.53 (s, 3 H). 
         [0137]      13 C NMR (100 MHz, acetone-d 6 ) δ 169.2, 165.2, 159.9, 157.7, 155.5, 149.6, 139.6, 136.3, 131.4, 128.9, 128.8, 128.4, 128.0, 125.2, 123.1, 116.6, 113.7, 68.5, 65.2, 43.1, 39.4, 14.7, −0.6, −1.9. 
         [0138]    HRMS (ESI): miz calc, for C 40 H 41 N 8 O 4 Si 725.3020; found 725.3032 (1.65 ppm), [M+H] +   
       B.4 SiR650-6CP (5.8 mg, 76%) 
       [0139]      1 H NMR (400 MHz, CD 3 OD) δ 8.32 (d, 1 H, J=8.2 Hz), 8.15 (dd, 1 H, J=8.2, 1.7 Hz), 7.74 (d, 1 H, J=1.4 Hz), 7.42−7.36 (m, 4 H, J=8.3 Hz), 7.33 (d, 2 H, J=2.7 Hz), 6.98 (d, 2 H, J=9.5 Hz), 6.76 (dd, 2 H, J=9.5, 2.8 Hz), 6.10 (s, 1 H), 5.35 (s, 2 H), 4.59 (s, 2 H), 3.31 (s, 12 H, overlapped with Me0H), 0.65 (s, 3 H), 0.60 (s, 3 H). 
         [0140]      13 C NMR (125 MHz, acetone-d 6 ) δ 170.8, 169.3, 165.2, 160.7, 155.5, 149.6, 140.2, 139.2, 136.2, 135.4, 131.4, 128.5, 128.4, 128.0, 127.95, 127.9, 125.2, 123.2, 116.5, 113.6, 95.3, 67.5, 43.0, 39.4, −0.6, −1.9. 
         [0141]    HRMS (ESI): Ritz calc. for C 39 H 40 ClN 6 O 4 Si 719.2569; found 719.2575 (0.84 ppm), [M+H] +   
       B.5 SiRMe-5BG (4.8 mg, 63%; Comparative Example) 
       [0142]      1 H NMR (400 MHz, CD 3 OD) δ 9.20 (t, 1 H, J=5.9 Hz), 8.27 (s, 1 H), 7.94 (s, 1 H), 7.89 (dd, 1 H, J=7.9, 1.3 Hz), 7.58 (d, 2 H, J=8.4 Hz), 7.47 (d, 2 H, J=8.2 Hz), 7.40 (d, 2 H, J=2.9 Hz), 7.28 (d, 1 H, J=7.9 Hz), 7.06 (d, 2 H, J=9.6 Hz), 6.79 (dd, 2 H, J=9.6, 2.9 Hz), 5.67 (s, 2 H), 4.68 (s, 2 H), 3.36 (s, 12 H), 2.12 (s, 3 H), 0.64 (s, 3 H), 0.62 (s, 3 H). 
         [0143]      13 C NMR (100 MHz, CD 3 OD) δ 168.0, 167.8, 159.8, 154.4, 152.8, 148.1, 142.2, 141.5, 140.6, 139.5, 136.3, 134.7, 134.3, 129.2, 128.8, 128.8, 127.4, 126.7, 124.4, 121.0, 114.0, 69.1, 42.9, 39.5, 18.1, −2.5, −2.7. 
         [0144]    HRMS (ESI): m/z calc. for C 40 H 43 N 8 O 2 Si 695.3278; found 695.3273 (−0.72 ppm), [M+H] +   
       B.6 SiRMe-5CP (5.0 mg, 66%; Comparative Example) 
       [0145]      1 H NMR (400 MHz, CD 3 OD) δ 7.93 (s, 1 H), 7.89 (m, 1 H), 7.44 (d, 2 H, J=3.9 Hz), 7.39 (d, 2 H, J=2.9 Hz), 7.27 (d, 1 H, J=7.9 Hz), 7.07 (d, 2 H, J=9.6 Hz), 6.80 (dd, 2 H, J=9.6, 2.9 Hz), 6.13 (s, 1 H), 5.38 (s, 2 H), 4.66 (s, 2 H), 3.37 (s, 12 H), 2.12 (s, 3 H), 0.64 (s, 3 H), 0.62 (s, 4 H). 
         [0000]      13 C NMR (101 MHz, CD 3 OD) δ 171.0, 167.9, 167.9, 154.4, 148.1, 142.2, 140.6, 138.8, 136.3, 135.4, 134.7, 129.2, 128.9, 128.2, 127.3, 126.7, 124.4, 120.9, 114.0, 95.2, 67.6, 43.0, 39.5, 18.1, −2.5, −2.7. 
         [0146]    HRMS (ESI); miz calc. for C 39 H 42 ClN 6 O 2 Si 689.2827; found 689.2839 (1.74 ppm), [M+H] +   
       B.7 SiRMe-6BG (3.9 mg, 51%; Comparative Example) 
       [0147]      1 H NMR (400 MHz, CD 3 OD) δ8.29 (s, 1 H), 7.99 (dd, 1 H, J=8.0, 1.9 Hz), 7.67 (d, 1 H, J=1.9 Hz), 7.54 (m, 3 H), 7,42 (d, 2 H, J=8,2 Hz), 7.40 (d, 2 H, J=2.9 Hz), 7.06 (d, 2 H, J=9.6 Hz), 6.79 (dd, 2 H, J=9.7, 2.9 Hz), 5.64 (s, 2 H), 4.59 (s, 2 H), 3.37 (s, 12 H), 2.12 (s, 3 H), 0.63 (s, 6 H). 
         [0000]      13 C NMR (100 MHz, CD 3 OD) δ167.7, 167.6, 159.8, 154.4, 152.6, 148.1, 141.7, 140.7, 139.9, 139.5, 139.1, 134.2, 131.6, 130.3, 128.8, 127.7, 127.5, 127.3, 126.9, 120.9, 114.0, 69.2, 42.9, 39.5, 18.1, −2.6, −2.6. 
         [0148]    HRMS (ESI): m/z calc. for C 40 H 43 N 6 O 2 Si 695.3278; found 695.3277 (−0.14 ppm), [M+H] +   
       B.8 SiRMe-6CP (5.5 mg, 72%; Comparative Example) 
       [0149]      1 H NMR (400 MHz, CD 3 OD) δ9.08 (t, 1 H, J=5.3 Hz), 7.99 (dd, 1 H, J=8.0, 1.8 Hz), 7.66 (d, 1 H, J=1.7 Hz), 7.55 (d, 1 H, J=8.0 Hz), 7.39 (m, 6 H), 7.07 (d, 2 H, J=9.7 Hz), 6.80 (dd, 2 H, J=9.7, 2.9 Hz), 6.10 (s, 1 H), 5,35 (s, 2 H), 4.59 (d, 2 H, J=5.7 Hz), 3.37 (s, 12 H), 2.12 (s, 3 H), 0.63 (s, 6 H). 
         [0000]      13 C NMR (100 MHz, CD 3 OD) δ 171.0, 167.9, 167.6, 154.5, 148.2, 140.7, 139.8, 139.1, 138.8, 135.4, 131.7, 130.3, 128.1, 127.8, 127.4, 127.0, 120.9, 114.0, 95.3, 67.6, 43.0, 39.5, 18.1, −2.6, −2.6. 
         [0150]    HRMS (ESI): m/z calc. for C 39 H 42 ClN 6 O 2 Si 689.2827; found 689.2822 (−0.73 ppm), [M+H] +   
       C. Live Cell Staining 
       [0151]    As a representative example for the labeling of a SNAP-tag fusion with BG-Sir650, the labeling and fluorescence imaging of SNAP-Cep41 (microtubule binding protein) fusion protein in U2OS cells is described. SNAP-Cep41 was expressed from episomal pEBTet plasmid encoding puromycin resistance under a doxycycline inducible promoter in U2OS cells. Prior to induction of expression, cells were selected by growing them in complete Dulbecco&#39;s Modified Eagle&#39;s Medium (DMEM) with 1 μg/ml puromycin for at least 1.5 weeks. SNAP-Cep41 fusion protein expression was induced for 48 h by adding to complete DMEM grow medium doxycycline at final concentration of 0.1 μg/ml before staining procedure. Living cells were stained by replacing old media with complete DMEM growth media containing 5 μM BG-SiR650, and incubated for 30 min at 37° C. in 5% CO 2  incubator. Cells were washed two times with Hank&#39;s Buffered Salt Solution (HBSS) for 5 min and once with media for 1 h. Before imaging, growth media was replaced with HBSS. Images ( FIG. 2A ) were acquired on a confocal fluorescence microscope as z-stacks and presented as maximum intensity projections. For the imaging of fixed cells, living cells were labeled with 1 μM BG-Sir650 for 30 min at 37° C. Cells were pre-extracted with BRB80 buffer containing 0.2% NP-40 and fixed with MeOH/EGTA at -20° C. for 5 min. Fixed cells were mounted in 90% glycerol containing 5% n-propyl-gallate as antifading. Images ( FIG. 2B ) were acquired on a confocal fluorescence microscope as z-stack and presented as maximum intensity projections. The image shown in  FIG. 2B  has been deconvolved using Huygens Essential software. 
       D. Photostability of the Dyes 
       [0152]    Comparative experiments of prior art dyes and a compound according to the invention are exemplarily outlined in  FIG. 3 . A. mAB-Alexa647 is goat anti-mouse IgG-Alexa Fluor 647 (highly cross-absorbed), purchased from Invitrogen (A-21236); mAb-Atto647N is goat anti-rabbit IgG-Atto647N (STED), purchased from Active Motif (15048); BG-SiR650 is B.3. 
         [0000]    Imaging conditions on Zeiss LSM 710 were as follows: 
       Objective: Plan-Apochromat 63×/1.00 Oil DIC M27 
     Pixel Size: 22×22 nm 
     Pinhole: 30 μm (0.5−0.6 AU) 
     Averaging: 8 
     Zoom: 24 
       [0153]    633 nm laser: 3% (36 μW reaching the objective, measured)
 
Detection interval: 640−758 nm
 
Mounting media: 86% glycerol+4% NPG in 1× PBS (Lonza)
 
         [0154]    As is readily apparent, the compound according to the invention possesses outstanding photostability, comparable to mAb-Atto647N.