Abstract:
New fluorinated benzodiazepins are described. They are expressed by the following general formula: ##STR1## wherein A 1  and A 2  are the same or different groups, each expressed by one of the following general formulas ##STR2## R 2  being a hydrogen atom, aliphatic hydrocarbon group, fluorine-substituted aliphatic hydrocarbon group, aminoalkyl, N,N-dialkylaminoalkyl, or alkylsulfonylalkyl group and R 3  a hydrogen atom, aliphatic or aromatic hydrocarbon group, halogen-substituted aliphatic or aromatic hydrocarbon group, while R 1  is a hydrogen atom, aliphatic or aromatic hydrocarbon group, fluorine-substituted aliphatic or aromatic hydrocarbon group and X is a hydrogen atom, aliphatic hydrocarbon group, fluorine-substituted aliphatic hydrocarbon group, halogen atom, nitro group or the like.

Description:
BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     This invention relates to new fluorinated benzodiazepins. 
     2. Description of the Prior Art 
     Some mono-fluorinated compounds are known to exhibit useful physiological activities. 
     OBJECTS AND SUMMARY OF THE INVENTION 
     It is an object of the present invention to provide new and useful fluorinated benzodiazepins. This object is accomplished according to the invention by fluorinated benzodiazepins of the formula. ##STR3## wherein A 1  and A 2  are the same or different groups, each expressed by one of the following general formulas ##STR4## R 2  being a hydrogen atom, aliphatic hydrocarbon group, fluorine-substituted aliphatic hydrocarbon group, aminoalkyl, N,N-dialkylaminoalkyl, or alkylsulfonylalkyl group and R 3  a hydrogen atom, aliphatic or aromatic hydrocarbon group, halogen-substituted aliphatic or aromatic hydrocarbon group, while R 1  is a hydrogen atom, aliphatic or aromatic hydrocarbon group, fluorine-substituted aliphatic or aromatic hydrocarbon group and X is a hydrogen atom, aliphatic hydrocarbon group, fluorine-substituted aliphatic hydrocarbon group, halogen atom, nitro group or the like. 
     The above fluorinated benzodiazepins are useful as physiologically active substances, such as insecticide and disinfectant, or intermediates to synthesize building blocks of these substances. 
     Other objects and advantages of the invention will become apparent from the following description of embodiments. 
     DESCRIPTION OF THE PREFERRED EMBODIMENTS 
     In the fluorinated benzodiazepins of the invention, the groups A 1  and A 2  respectively contain a nitrogen atom necessary to form a diazepin ring. Basically, they are provided in one of the following three forms ##STR5## 
     Therefore, there is possible to be a variety of the fluorinated benzodiazepins. Among these compounds, preferred is compound of the formula ##STR6## 
     Among the group R 2  is preferred a hydrogen atom, alkyl, fluoralkyl, alkenyl, aminoalkyl, N,N-dialkylaminoalkyl, or alkylsulfonylalkyl group, containing up to ten carbon atoms. Examples of such R 2  are hydrogen atom, methyl, ethyl, propyl, butyl, c-propylmethyl, trifluoroethyl, N,N-diethylaminoethyl, and methylsulfonylethyl groups. 
     Among the group R 3  is preferred a hydrogen atom, alkyl, fluoroalkyl, phenyl, fluorophenyl or chlorophenyl group containing up to ten carbon atoms. The R 3  may thus be selected among the alkyl, fluoroalkyl, alkenyl and fluoroalkenyl groups cited above for the group R 2 . For example, it may represent a trifluoromethyl group. Further examples of the R 3  are phenyl and other aryl groups which may have floro, chloro and/or fluoroalkyl substituent or substituents. For example, it may be ##STR7## 
     The group R 1  is preferably a hydrogen atom, alkyl, fluroalkyl, alkenyl or fluoroalkenyl group having up to five carbon atoms. The R 1  may thus be one of the alkyl, fluroalkyl, alkenyl and fluoroalkenyl groups cited above for the R 2  and R 3  as far as the number of carbon atoms are limited to five. For example, methyl, ethyl, propyl and butyl groups are preferable. Further, the group R 1  may be a phenyl, fluorophenyl or other aryl group, or even an aralkyl group. 
     It is noted that in the above fluorinated groups, for example, in the &#34;fluoralkyl group&#34; the number of substituent fluorine atoms and their positions may be variously changed. 
     Further, in the above general formula representing the fluorinated benzodiazepins of the invention, the substituent X on the benzene ring is preferably one of the alkyl and alkenyl groups having up to five carbon atoms as cited above for R 1 , for example, methyl group, or halogen atom, such as chlorine or fluorine atom, nitro group or the like. 
     At least having a fluorine atom at the 3-position of their heterocyclic ring, the fluorinated benzodiazepins of the invention are physiologically active and expected to have other properties that are characteristic of the fluorinated compound. 
     The fluorinated benzodiazepins of the invention can be prepared by a process defined by a series of reaction formulas as given next. First, hexafluoropropene (CF 3  CF═CF 2 ) is reacted in a solution of sodium ethoxide in ethanol to form an adduct 1, which is then hydrolized to an ester 2 by sulfuric acid or the like. This ester is again reacted in a solution of sodium ethoxide in ethanol for conversion of its CF 3  group to an ester group to produce fluoromalonic ester 3. The ester 3 is alkylated by the ordinary method to synthesize an alkylated product 4. The α-fluoro-β-diesters 3 and 4 thus prepared can be used for the synthesis of fluorinated benzodiazepins as mentioned later. ##STR8## 
     Meanwhile, according to the following series of reaction formulas, trifluoroethene (CHF═CF 2 ) is reacted with acetyl chloride in presence of AlCl 3  to form an adduct 5, which is then alcoholized to give α-fluro-β-ketoester 6 that can be used for synthesis of fluorinated benzodiazepins of the invention. ##STR9## 
     Further, according to the following series of reaction formulas, an adduct 7 and substitution product 8 are produced by the reaction of hexafluoropropene and diethylammonium and they are hydrolized to an amide 9, which is then reacted with R 3  MgBr wherein R 3  is one of the preferable groups as cited above for R 3  to produce a ketone 10 that can be alcoholized by the ordinary method to α-fluorobenzoylacetic ester 11. ##STR10## 
     The above products 3, 4, 6 and 11 can be used to synthesize fluorinated benzodiazepins of the invention by methods as described below. 
     Synthesis of 1H-3-fluoro-1,5-benzodiazepin-2,4(3H,5H)-dione 12 
     O-phenylenediamine and fluoromalonic ester 3 are dissolved in a solution of sodium ethoxide in ethanol and the solution is refulxed for 1 to 10 hours for a reaction to produce a benzodiazepin 12 in high yield according to the following reaction formula. The alkylated product 4 of fluoromalonic ester 3 is likewise reacted to give the corresponding benzodiazepin 12 in high yield. ##STR11## 
     Solvents applicable for this reaction can be aprotic polar solvent, for example, dimethylformamide, dimethylsulfoxide and sulfolane wherein bases such as Na, K, NaH are used. Reaction temperature can be 50° to 150° C. and reaction pressure can be atmospheric or reduced pressure. 
     When the R 1  was variously changed in the above reaction, the target compound 12 was produced in high yield as the corresponding products as listed in the following table: 
     
         ______________________________________Product 12 Yield   M.P.    IR (cm.sup.-1)                               .sup.19 FNMRR.sup.1  X       (%)     (°C.)                        (CO) (NH)  ppm (.sup.J H-F)______________________________________H      H       61      &gt;300  1680 3200  132.1d(J39.5)H      CH.sub.3 --          72      &gt;300  1670 3260  130.0d(J39.5)H      Cl      44      &gt;300  1690 3340  129.5d(J39.5)H      NO.sub.2          52       262  1690 3350  119.5d(J39.5)CH.sub.3 --  H       59       245  1680 3200   78.0q(J20.7)C.sub.2 H.sub.5 --  H       62       283  1660 3170   87.5t(J19.8)C.sub.4 H.sub.9 --  H       48       204  1690 3200   85.5t(J18.8)______________________________________ 
    
     Further, when reacted with R 2  Y, wherein R 2  is one of the groups as mentioned above for such and Y is a halogen atom, for example, I, the benzodiazepin 12 is readily substituted with a group R 2  according to the following formula. For example, when reacted with CH 3  I, it is methylated. A protected and stabilized benzodiazepin derivative 13 can thus be prepared. ##STR12## 
     Solvents applicable for this reaction can be alcohol such as ethanol as well as aprotic polar solvent already described. Reaction temperature can be 20° to 100° C. and reaction pressure can be atmospheric or reduced pressure. The same benzodiazepin 12 can also be converted to other benzodiazepin 14 or 15 according to the following reaction formulas: ##STR13## 
     Synthesis of 1H-3-fluoro-4-phenyl-1,5-benzodiazepin-2-one 16 
     O-phenylenediamine and α-fluorobenzoylacetic ester 11 are put in a mixed solvent of CH 3  COOH/C 2  H 5  OH and refluxed to form a benzodiazepin 16 in high yield (for example, 43 to 65%) according to the following reaction formula. ##STR14## 
     It is noted that the phenyl group Ph on the heterocyclic ring of the product 16 may be a different R 3  as mentioned above. For example, a benzodiazepin 17 wherein R 3  =CH 3  --, can be prepared by the following reaction: ##STR15## 
     Solvent usable for the reactions to produce 16 and 17 can be aprotic polar solvent already described, reaction temperatures can be 50° to 150° C. and reaction pressure can be atmospheric. 
     On the other hand, β-perfluoroalkyl-β-ketoesters 11&#39; and 11&#34; can be prepared by the crossed Claisen condensation according to the following reaction formulas, which may also be used for synthesis of fluorinated benzodiazepins of the present invention. ##STR16## 
    
    
     The present invention will be more clearly understood with reference to the following examples. 
     EXAMPLE 1 
     O-phenylenediamine (1.1 g, 10 mmol) and ethyl fluoromalonate (1.8 g, 10 mmol) were added to a solution of sodium ethoxide (0.68 g, 10 mmol) in ethanol (10 ml). The mixture was refluxed 5 hours and left to stand overnight at room temperature. The solution was then acidified by hydrochloric acid and precipitates were separated by filtration. Recrystallization from acetic acid gave 1.2 g of a white crystalline product of a melting point above 300° C. (61%). 
     EXAMPLE 2 
     P-chloro-o-phenylenediamine (1.5 g, 10 mmol) and ethyl fluoromalonate (1.8 g, 10 mmol) were added to a solution of sodium ethoxide (0.68 g, 10 mmol) in ethanol (10 ml). The same process as applied to Example 1 gave 1.0 g of a crystalline product of a melting point above 300° C. (44%). 
     EXAMPLE 3 
     P-nitro-o-phenylenediamine (1.6 g, 10 mmol) and ethyl fluoromalonate (1.8 g, 10 mmol) were added to a solution of sodium ethoxide (0.68 g, 10 mmol) in ethanol (10 ml). The same process as applied to Example 1 was repeated except that methanol was used for recrystallization. There was produced 1.3 g of crystalline product of a melting point 262° (52%). 
     EXAMPLE 4 
     P-methyl-o-phenylenediamine (1.3 g, 10 mmol) and ethyl fluoromalonate (1.8 g, 10 mmol) were added to a solution of sodium ethoxide (0.68 g, 10 mmol) in ethanol (10 ml). The same process as applied to Example 1 was repeated except that methanol was used for recrystallization. There was produced 1.5 g of a crystalline product of a melting point above 300° C. (72%). 
     EXAMPLE 5 
     O-phenylenediamine (1.1 g, 10 mmol) and ethyl 2-fluoro-2-methylmalonate (1.95 g, 10 mmol) were added to a solution of sodium ethoxide (0.68 g, 10 mmol) in ethanol (10 ml). The mixture was refluxed 5 hours and left to stand overnight at room temperature. The solution was then acidified by hydrochloric acid and precipitates were separated by filtration. Recrystallization from methanol gave 1.25 g of a crystalline product of a melting point above 245° C. (59%). 
     EXAMPLE 6 
     In Example 5, ethyl 2-fluoro-2-ethylmalonate (2.15 g, 10 mmol) was used in place of ethyl 2-fluoro-2-methylmalonate. The same procedure as Example 5 were performed. Recrystallization from ethanol gave a product of melting point 283° C. in a yield of 62%. 
     EXAMPLE 7 
     O-phenylenediamine (1.1 g, 10 mmol) and ethyl 2-fluoro-2-butylmalonate (2.35 g, 10 mmol) were added to a solution of sodium ethoxide (0.68 g, 10 mmol) in ethanol (10 ml). The same process as applied to Example 5 was repeated except that water was used for recrystallization. There was given 1.25 g of a crystalline product of a melting point 204° C. (48%) 
     EXAMPLE 8 
     Methyl iodide (2.9 g, 20 mmol) was added dropwise in half an hour to a solution of 1H-3-fluoro-1,5-benzodiazepin-2,4(3H,5H)-dione (1.95 g, 10 mmol) and sodium ethoxide (0.68 g, 10 mmol) in ethanol (20 ml). The mixture was refluxed 3 hours. After cooling, it was acidified and precipitates were separated by filtration. Recrystallization from methanol gave 1.2 g of a needle-like crystalline product of a melting point above 300° C. (55%). This product showed the following analytical data. 
     Anal. Found: C,59.21; H,4.87; N,12.74%. Calcd. for C 9  H 11  N 2  O 2  F. C,59.45; H,4.98; N,12.60%. 
     IRνmax. (KBr) 1722,1700,1600 cm -1 . (CO,C=N, stretching). 
       19  F NMR δ(neat)+126.o,d,J=45.16 Hz,1F,CHF. 
       1  H MNR δ(DMSO-d 6 ) 3.3,s,6H,2CH 3  ; 5.33, d,J=48.5 Hz, 1H, CHF; 7.19,s,4H,Ar--H. 
     EXAMPLE 9 
     Ethyl 1,1,1-trifluoro-3-fluoro-acetylacetate (1.85 g, 10 mmol) and o-phenylenediamine (1.1 g, 10 mmol) were added in a mixed solvent (25 ml of ethyl alcohol plus 10 ml of acetic acid). After refluxing 5 hours, solvent was evaporated. Recrystallization of residues from ethanol gave 1.85 g of a crystalline product of a melting point 165° C. (80% ). 
     EXAMPLE 10 
     Using ethyl 1,1,1-trifluoro-3-fluoro-acetylacetate (1.85 g, 10 mmol) and p-methyl-o-phenylenediamine (2.45 g, 10 mmol), the process of Example 8 was repeated. There was thus produced 2.3 g of a crystalline product of a boiling point 198° C. (95%). 
     EXAMPLE 11 
     Using ethyl 1,1,1-trifluoro-3-fluoro-acetylacetate (1.85 g, 10 mmol) and p-chloro-o-phenylenediamine (2.65 g, 10 mmol), the process of Example 8 was repeated. There was produced 2.1 g of a crystalline product of a melting point 237° C. (75%). 
     EXAMPLE 12 
     O-phenylenediamine (1.08 g, 10 mmol) and trifluorobenzoylacetone (2.0 g, 10 mmol) were suspended in acetic acid and heated 3 hours at 80° C. After the mixture cooled down, it was poured into iced water and precipitates thereby formed were separated by filtration. Recrystallization from chloroform gave 1.9 g of a crystalline product of a melting point of 208° C. (65%). 
     Anal. Found: Calcd. for C 16  H 11  N 2  F 3 . C,66.66; H,3.84; N,9.71%. 
     IR ν max. (KBr) 1598 cm -1 . (C=N, stretching). 
       19  FδNMR (neat) -13.2s,3F,CF 3 . 
       1  HδNMR (DMSO-d 6  /CCl 4 ) 3.4,s,2H,CH 2  ; 7.33-8.0,m,9H,Ar--H. MS (m/e) 288 (M + ) 
     EXAMPLES 13 to 15 
     Refluxing of ethyl α-fluorobenzoylacetate (2.25 g, 10 mmol) with o-phenylenediamine (10 mmol) in the mixture of acetic acid and ethanol (25%) for 5 h gave 3-fluoro-1,5-benzodiazepin-2-one derivatives in good yields as follows. ##STR17## wherein X=H, 4-Me, 4-Cl or NO 2 . It is clear that the first step of this reaction is condensation of carbonyl ketone and amine by losing water and second step is cyclization of their ester with another amine by losing ethanol. When used p-CH 3  or p-Cl o-phenylenediamine, these were condensed with α-fluorobenzoylacetate, 
     Products in the above Examples as well as other products that were produced according to the invention are listed in Tables 1, 2, and 3 together with their physical and analytical data. 
     It will be evident that various modifications can be made to the described embodiments without departing from the scope of the present invention. 
     
                                           TABLE 1__________________________________________________________________________ ##STR18##             IR    NMR                     Elementary analysisExampleProduct M.P. Yield             (cm.sup.-1)                   19.sub.F                           1.sub.H         theor. values in                                           parenthesesNo.  R.sup.1   X  (°C.)          (%)             [CO]                [NH]                   δ ppm(.sup.J HF)                           δ ppm     C (%)                                                H (%)                                                     N__________________________________________________________________________                                                     (%)1    H  H  &gt;300          61 1680                3200                   132.1 d(J 39.5)                           5.45 d(CHF), 7.16 s(ArH)                                           56.03                                                3.65 14.54                           10.76 s(NH × 2)                                           (55.67)                                                (3.63)                                                     (14.43)4    H  Me &gt;300          72 1670                3260                   130.0 d(J 39.5)                           2.20 s(CH.sub.3), 5.45 d(CHF)                                           57.60                                                4.59 13.32                           7.07 s(ArH), 10.50 s(NH                                           (57.69) 2)                                                (4.36)                                                     (13.43)2    H  Cl &gt;300          44 1690                3340                   129.5 d(J 39.5)                           5.44 d(CHF), 7.11 s(ArH)                                           47.03                                                2.48 11.99                           10.30 s(NH × 2)                                           (47.20)                                                (2.64)                                                     (12.25)3    H  NO.sub.2       262          52 1690                3350                   119.5 d(J 39.5)                           5.70 d(CHF), 7.40˜8.10                                           44.54)                                                2.53 17.29                           10.45 s(NH × 2)                                           (45.19)                                                (2.52)                                                     (17.56)5    Me H   245          59 1680                3200                   78.0 q(J 20.7)                           1.33 d(CH.sub.3), 7.16 s(ArH)                                           57.67                                                4.57 13.31                           10.69 s(NH × 2)                                           (57.69)                                                (4.36)                                                     (13.45)6    Et H   283          62 1660                3170                   87.5 t(J 19.8)                           0.87 t(CH.sub.3), 1.80 dq(CH.sub.2)                                           59.34                                                4.91 12.56                           7.23 s(ArH), 10.98 s(NH                                           (59.46) 2)                                                (4.99)                                                     (12.61)7    Bu H   204          48 1690                3200                   85.5 t(J 18.8)                           0.66 t(CH.sub.3), 1.00˜1.30                           m(C.sub.2 H.sub.4)                                           62.15                                                6.06 11.13                           1.60 dt(CFC  .sub.--H.sub.2)                                           (62.38)                                                (6.04)                                                     (11.19)                           7.17 s(ArH), 10.90 s(NH__________________________________________________________________________                           × 2) 
    
     
                       TABLE 2______________________________________ ##STR19##                         IRExample  Product  M.P.    Yield (cm.sup.-1)No.    X        (°C.)                   (%)   [CN]    [CO]  [NH]______________________________________ 9     H        165     80    1600    1680  321010     CH.sub.3 198     95    1605    1685  320011     Cl       237     75    1600    1690  3200______________________________________ 
    
     
                                           TABLE 3__________________________________________________________________________ ##STR20##            IR         NMR                       MSExampleProduct     M.P.        Yield            (cm.sup.-1)                       .sup.19 F                               .sup.1 H          (m/e)No.  X    (°C.)        (%) [CN] [CO]                    [NH]                       δ ppm(.sup.J HF)                               δ ppm       [M.sup.+ ]__________________________________________________________________________13   H    178        53  1605 1685                    3400                       124 d(41.4)                               5.45 d(CHF), 7.30˜8.20                                                 254rH)                               11.17 s(NH)14   CH.sub.3     188        61  1620 1690                    3430                       123 d(43.2)                               2.40 s(CH.sub.3), 5.51d(CHF)                                                 268                               7.00˜8.05 m(ArH), 10.93 s(NH)15   Cl   252        45  1600 1680                    3420                       122 d(41.8)                               5.56 d(CHF), 7.17˜8.28 m(ArH)                               11.23 s(NH)__________________________________________________________________________