Abstract:
The present invention relates to an apparatus for partitioning a sampling region on a solid surface and a method for collecting a microbial sample from a solid surface, in which an optimal sampling region is partitioned on a solid surface using a light source without bringing the solid surface into direct contact with a ruler or a partitioning tool. With the apparatus and method, a solid surface can be prevented from being contaminated during partitioning process, accuracy of sampling operation can be increased, partitioning operation can be continuously performed in a simple manner, and cost of sampling operation can be reduced.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
       [0001]    The present application is a continuation of PCT/KR2011/010171 filed Dec. 27, 2011, which claims priority to Korean Application No. 10-2010-0136255 filed Dec. 28, 2010 and Korean Application No. 10-2011-0143022 filed Dec. 27, 2011, which applications are incorporated herein by reference. 
     
    
     TECHNICAL FIELD 
       [0002]    The present invention relates to collecting a microbial sample for use in the preparation of a test sample solution provided in the microbial test method of the Korean Food Standards Codex, and more particularly to a method for collecting a microbial sample from a solid surface using a contactless partitioning system and to an apparatus for partitioning a sampling region on a solid surface, wherein the sampling region for microbial analysis is partitioned on the solid surface using a light source without bringing the light source in contact with the solid surface, and thus it is possible to prevent microbial contamination which occurs when the partitioning operation is performed using a template, and the sampling operation can be simplified, resulting in a significant reduction in the sampling time, and furthermore, the partitioning apparatus can be used multiple times, resulting in a reduction in the cost incurred in the sampling operation. 
       BACKGROUND ART 
       [0003]    The Korean Food Sanitation Act provides that the Commissioner of the Korea Food and Drug Administration shall determine and publicly announce standards for manufacturing, processing, cooking or storing foods or food additives, when necessary for public health (Food Standards Codex). 
         [0004]    With this Food Standards Codex, Article 7 of the Korean Food Sanitation Act provides standards for manufacturing, processing, using, cooking and storing foods, food additives, devices, containers and packages, and standards for ingredients. In addition, the Korean Food Sanitation Act provides standards and specifications for 20 food groups, 138 food classes and 480 food types, standards and specifications for devices, containers and packages according to 45 kinds of materials, and testing methods therefor. 
         [0005]    The Korean Food Standards Codex was provided based on the Korean Food Sanitation Act established on January 20, 1962 and was first opened in the year 1966 by announcing standards and specifications for alcoholic beverages and soy source. 
         [0006]    Since then, the management system of the Korean Food Standards Codex was established in December 23, 1967 by announcing standards for the manufacture, processing and use of soy source and provisions for the ingredients thereof under Decree No. 206 of the Ministry of Health and Social Affairs. In the year 1976, the Korean Food Sanitation Act was amended while the standards and specifications determined by the decrees of the Ministry of Health and Social Affairs were changed to those determined by the notices of the Ministry of Health and Social Affairs, and in the year 1977, standards and specifications for foods and the like were wholly amended. to provide a basis on which the current Korean Food Sanitation Act is based. Since then, the Korean Food Standards Codex was amended several times, and with the launch of the WTO, it was converted to a management system that loosens quality standards while enforcing safety. With the loosening of quality standards, the desire to develop new products has been boosted. In addition, the Korean Food Standards Codex has been continually amended such that it could be harmonized with international standards. 
         [0007]    Moreover, the Korean Food Standards Codex includes general provisions, methods for the collection and handling of samples, common standards and specifications for general foods, standards and specifications for each food, recommended microbial standards for foods to be cooked and marketed by food service establishments, provisional standards for marine products, standards and specifications for devices, containers and packages, general testing methods, reagents, sample solutions, standard solutions, standard solution for volumetric analysis, and appenda. 
         [0008]    In the Korean Food Standards Codex, sampling methods are described according to the kind of sample (heterogeneous food, sample for microbial analysis, package sample, fishes and shells, etc.). 
         [0009]    Among them, the sampling methods for microbial analysis include various sampling methods for liquid samples, semi-solid samples, solid samples, solid surface samples, and powder samples. 
         [0010]    In the sampling methods for solid surface samples, a specific area is partitioned on the solid surface, and a sample is collected from the partitioned area and used as a microbial test solution. 
         [0011]    For the solid surface sampling as described above, a region having a specific size should be partitioned on the solid surface. In order to partition a region having specific size on the solid surface, the region having a specific size is partitioned on the solid surface using a ruler and a pen, and the partitioned region is sampled. Alternatively, a separately prepared template is attached to the solid surface, and the region partitioned thereby is sampled. In other words, a plurality of partitioned regions having a constant size are sampled to obtain standards. 
         [0012]    However, in the conventional sampling methods as described above, tools, such as a ruler, a pen or a template, are used, and thus there are serious problems in that the tools cause instantaneous microbial contamination when they come into contact with the solid surface, and furthermore, result in the error of microbial sampling. 
         [0013]    In recent years, in order to overcome the above-described problems, a template sterilized by gamma radiation has been used. This template can minimize contamination caused by a tool used to partition a sampling region on the solid surface. 
         [0014]    The above-described sterilized template can minimize the contamination of the solid surface, because it is used after sterilization. However, it is expensive because it is sterilized. Further, it is disposable and non-recyclable, and thus when several regions of the solid surface are to be sampled, a plurality of the templates should be used, resulting in an increase in analysis cost. In addition to these problems, the template should be attached to each region, making the analysis process very complicated. 
       SUMMARY OF THE DISCLOSURE 
       [0015]    The present invention has been made in order to solve the above-described problems, and it is an object of the present invention to provide a method for collecting a microbial sample from a solid surface using a contactless partitioning system and an apparatus for partitioning a sampling region on a solid surface, in which an optimal sampling region is partitioned on a solid surface using a light source without bringing the solid surface into direct contact with a ruler or a partitioning tool, and thus the solid surface can be prevented from being contaminated during the partitioning process, the accuracy of the sampling operation can be increased, the partitioning operation can be continuously performed in a simple manner so as to reduce the time of the sampling operation, and particularly the apparatus can be used many times, resulting in a reduction in the cost of the sampling operation. 
         [0016]    To achieve the above object, the present invention provides A method for collecting a microbial sample from a solid surface using a contactless partitioning method, the method comprising steps of: projecting a figure-shaped partitioned image corresponding to a sampling region onto the solid surface which is contactless with a light from a projecting light source; determining the figure-shaped sampling region projected on the solid surface by controlling the light source; collecting microorganisms from the sampling region by wiping the partitioned sampling region with a collection means; and preparing a suspension of the microorganisms by mixing and strongly shaking the collection means for collecting microorganisms with sterile physiological saline. 
         [0017]    The present invention also provides an apparatus for contactlessly partitioning a sampling region on a solid surface, the apparatus comprising: a body; a battery for supplying power included in the body; an operating switch for turning ON/OFF power at the top; a light source at the bottom of the body which downwardly irradiates a light; and a partitioning means for partitioning a figure-shaped sampling region below the light source wherein the light irradiated from the light source through the partitioning means projects an image corresponding to a sampling region onto the solid surface. 
         [0018]    When the above-described method for collecting a microbial sample from a solid surface using a contactless partitioning system and the above-described apparatus for partitioning a solid surface are used, a figure-shaped sampling region is projected and partitioned on a solid surface using a light source, and thus an optimal sampling region from which a sample is to be collected can be partitioned on the solid surface. Also, because the contactless method is used, the solid surface can be prevented from being contaminated by the partitioning apparatus, and thus the accuracy of the sampling operation can be increased and the sampling time can be significantly reduced. 
         [0019]    In addition, because the apparatus can be used according to the ON/OFF operation of the light source, it can be continuously used several times, and thus the sampling operation cost caused by the use of a partitioning tool can be reduced. 
     
    
     
       BRIEF DESCRIPTION OF THE DRAWINGS 
         [0020]      FIG. 1  is a flow chart showing a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0021]      FIG. 2  shows a first embodiment of a sampling region partitioned using a two-part lamp-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0022]      FIG. 3  shows a second embodiment of a sampling region partitioned using a two-part lamp-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0023]      FIG. 4  shows a first embodiment of a sampling region partitioned using a four-part lamp-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning method according to the present invention. 
           [0024]      FIG. 5  shows a second embodiment of a sampling region partitioned using a four-part lamp-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning method according to the present invention. 
           [0025]      FIG. 6  shows a first embodiment of a sampling region partitioned using a single lamp-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0026]      FIG. 7  shows a second embodiment of a sampling region partitioned using a single lamp-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0027]      FIG. 8  shows an example of a sampling region partitioned using a single or four-part lamp-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0028]      FIG. 9  shows a first embodiment of a sampling region partitioned using a laser-type light source with an auxiliary light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0029]      FIG. 10  shows a second embodiment of a sampling region partitioned using a laser-type light source with an auxiliary light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0030]      FIG. 11  shows a first embodiment of a sampling region partitioned using a laser-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0031]      FIG. 12  shows a second embodiment of a sampling region partitioned using a laser-type light source in a method for collecting a microbial sample from a solid surface using a contactless partitioning system according to the present invention. 
           [0032]      FIG. 13  is a perspective view showing a first embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0033]      FIG. 14  is a cross-sectional view showing a first embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0034]      FIG. 15  is a perspective view showing a second embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0035]      FIG. 16  is a perspective view showing a third embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0036]      FIG. 17  is a top view showing a first embodiment of a filter in the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0037]      FIG. 18  is a top view showing a second embodiment of a filter in the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0038]      FIG. 19  is a cross-sectional view showing a fourth embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0039]      FIG. 20  is a top view showing a sampling region according to a fourth embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0040]      FIG. 21  is a cross-sectional view showing a sampling region according to a fifth embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
           [0041]      FIG. 22  is a top view showing a sampling region according to a fifth embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
       
    
    
     DESCRIPTION OF REFERENCE NUMERALS USED IN THE DRAWINGS  
       [0042]      10 : sampling region 
         [0043]      20 : a partitioned image 
         [0044]      100 : body 
         [0045]      110 : battery 
         [0046]      120 : operating switch 
         [0047]      130 : light source 
         [0048]      131  and  131 ′: lamp-type light source 
         [0049]      132 : main light source 
         [0050]      132   a : central point 
         [0051]      133 : auxiliary light source 
         [0052]      133   a : auxiliary point 
         [0053]      135 : straight light source 
         [0054]      140 : partitioning means 
         [0055]      141  and  141 ′: colored filter 
         [0056]      142 : partitioning portion 
         [0057]      142   a  and  142   a ′: partitioning line 
         [0058]      142   b  and  142   b ′: partitioning surface 
         [0059]      145 : diffraction lens; P: solid surface 
         [0060]    S 100 : light-projecting step 
         [0061]    S 200 : sampling region-forming step 
         [0062]    S 300 : microorganism-collecting step 
         [0063]    S 400 : suspension-preparing step 
       DETAILED DESCRIPTION 
       [0064]    The terms or words used in the specifications and claims should not be limited to be construed as usual or dictionary definition but should be rather construed to be consistent with the technical spirits of the present invention based on the principle that the inventors may properly define the terms used in the specification to describe their invention in the best manner. 
         [0065]    Accordingly, it should be understood that the embodiments described in the specification and configurations disclosed in the drawings are merely examples and do not represent all of the technical spirits of the invention and various modifications and variations to the invention and equivalents thereof may be made at the time of the invention. 
         [0066]    Hereinafter, preferred embodiments of the inventive method for collecting a microbial sample from a solid surface using a contactless partitioning system will be described in detail with reference to the accompanying drawings. 
         [0067]      FIG. 1  is a flow chart showing the inventive method for collecting a microbial sample from a solid surface using a contactless partitioning system. 
         [0068]    As shown in  FIG. 1 , the inventive method for collecting a microbial sample from a solid surface using a contactless partitioning system comprises the sequential steps of: (S 100 ) projecting a partitioned image  20  on a solid surface P using a light source; (S 200 ) controlling the partitioned image  20  to form a sampling region; (S 300 ) collecting microorganisms from the sampling region; and (S 400 ) preparing a suspension of the collected microorganisms. 
         [0069]    In the method of the present invention, the partitioned image-projecting step (S 100 ) is performed to project a light source onto the solid surface P in order to form a sampling region  10 . The step (S 100 ) is performed by irradiating the solid surface with light from a light source spaced apart from the solid surface and projecting the figure-shaped partitioned image, which corresponds to the sampling region, onto the solid surface (P) using the irradiated light. 
         [0070]    Also, the sampling region-forming step (S 200 ) is performed by controlling the light irradiated onto the solid surface (P). Specifically, the distance between the solid surface (P) and the light source is controlled to partition the figure-shaped sampling region  10  corresponding to an area for collecting microorganisms from the solid surface (P). 
         [0071]    Also, the microorganism-collecting step (S 300 ) is performed to collect microorganisms from the partitioned sampling region  10 . In this step, microorganisms are collected by wiping the sampling region  10  with a collection means. Herein, the collection means that is used to collect microorganisms may be a sterile gauze or cotton swab wetted with 1-5 ml of sterile physiological saline. 
         [0072]    In order words, microorganisms present in the sampling region are collected by adsorption using the sterile gauze or cotton swab. 
         [0073]    Moreover, the suspension-preparing step (S 400 ) is performed by mixing the collection means, used to collect microorganisms in the microorganism collection step (S 300 ), with sterile physiological saline, and strongly shaking the mixture to prepare a suspension of the collected microorganisms. Specifically, the step (S 400 ) is performed by mixing the collection means such as the sterile gauze or cotton swab with 10-100 ml of sterile physiological saline. 
         [0074]    In other words, the process of collecting the microbial sample is performed by mixing the collected microorganisms with sterile physiological saline in a conventional Erlenmeyer flask or test tube (not shown in the drawings) and preparing a suspension of the microorganisms from the mixture using a shake culture method which is applied for cell culture. 
         [0075]    Meanwhile, in the sample collection method as described above, the sampling region  10  may have various shapes. In the present invention, a square shape and a circular shape are preferably applied. Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. 
         [0076]    First, in the step (S 100 ) of projecting light from the light source, light from a two-part lamp-type light source which emits diffused light is passed through a filter to project a linearly partitioned image  20 . 
         [0077]    In the step (S 200 ) of forming the sampling region, the distance between the light source and the solid surface (P) is controlled, so that a linearly partitioned region having an area corresponding to the sampling region  10  can be formed when the opposite ends of two partitioned images  20  and  20 ′ projected from the light sources first come in contact with each other. 
         [0078]    In other words, when the sampling region  10  is to be partitioned by a square shape as shown in  FIG. 2 , the light source on one side (left) may project a partitioned image having a “         ” shape onto the solid surface P, and the light source on the other side (right) may project a partitioned image  20 ′ having a “         ” onto the solid surface P. 
         [0079]    In the step (S 200 ) of forming the sampling region, the two partitioned images  20  and  20 ′ projected from the light sources are controlled to be enlarged so that a sampling region  10  having a “         ” shape can be formed when the opposite ends of the partitioned images  20  and  20 ′ first come into contact with each other. 
         [0080]    Further, when the sampling region  10  is to be partitioned by a circular shape as shown in  FIG. 3 , the light source on one side (left) may project a partitioned image having a “         ” shape onto the solid surface P, and the light source on the other side (light) may project a partitioned image having a “         ” shape onto the solid surface P. 
         [0081]    In the step (S 200 ) of forming the partitioned region, the two partitioned images  20  and  20 ′ projected from the light sources are controlled to be enlarged, so that a sampling region  10  having a “         ” shape can be formed when the opposite ends of the partitioned images  20  and  20 ′ first come into contact with each other. 
         [0082]    In another embodiment, light from a four-part lamp-type light source which emits diffused light is passed through a filter to project linearly partitioned images  20  and  20 ′. 
         [0083]    In the step (S 200 ) of forming the sampling region, the distance between the light source and the solid surface (P) is controlled, so that a linearly partitioned sampling region having an area corresponding to the sampling region  10  can be formed when the opposite ends of the partitioned images  20  and  20 ′ projected from the light sources first come into contact with each other. 
         [0084]    Specifically, when the sampling region  10  is to be partitioned by a square shape as shown in  FIG. 4 , partitioned images  20  and  20 ′ having shapes of “         ”, “         ”, “         ” and “         ”, respectively, are projected using four radially filtered lamp-type light sources in the step (S 100 ) of projecting light images. In the step (S 200 ) of forming the sampling region, the distance between the four partitioning images  20  and  20 ′ projected from the light sources and the solid surface P is controlled so that a sampling region  10  having a “         ” shape can be partitioned when the ends of the partitioned images  20  and  20 ′ first come into contact with each other. 
         [0085]    Also, when the sampling region  10  is to be partitioned by a circular shape as shown in  FIG. 5 , partitioning images  20  and  20 ′ having shapes of “         ”, “         ”, “         ” and “         ” are projected four radially filtered lamp-type light sources in the step (S 100 ) of projecting light images. In the step (S 200 ) of forming the sampling region, the distance between the four partitioning images  20  and  20 ′ projected from the light sources and the solid surface P is controlled so that a sampling region  10  having a “         ” shape can be partitioned when the ends of the partitioned images  20  and  20 ′ first come into contact with each other. 
         [0086]    In another embodiment, light from a single lamp-type light source is passed through a filter to project a linearly partitioned image  20 . In step (S 200 ) of forming the sampling region, the distance between the partitioned image  20  projected from the light source and the solid surface P is controlled so that a linearly partitioned sampling region  10  can be formed by an enlarged image. 
         [0087]    Specifically, as shown in  FIGS. 6 and 7 , one filtered lamp-type light source is used in the step (S 100 ) of projecting the light source. The light source projects a partitioned image  20  having any one shape of “         ” and “         ” through a filter. In the step (S 200 ) of forming the sampling region, the partitioned image projected from the light source is controlled to be enlarged, so that a sampling region  10  having any one shape of “         ” and “         ” may be formed. 
         [0088]    Meanwhile, in the step (S 100 ) of projecting the light source, light from the light source may be passed through a filter to project a partitioned surface image  20 . 
         [0089]    As shown in  FIG. 8 , in the step (S 200 ) of forming the sampling region, the distance between a partitioning image  20  projected from a light source and the solid surface P is controlled so that a figure-shaped sampling region  10  having any one surface shape of “         ” and “         ” can be partitioned by the enlarged image. 
         [0090]    In addition, a single laser-type main light source having straightness and an auxiliary light source may be used. Herein, the main light source projects a figure-shaped partitioned image  20  having a central point  132   a  through a diffraction lens that emits diffused light, and the auxiliary light source projects a slant auxiliary point  133   a  that progresses toward the central point  132   a.  In the step (S 200 ) of forming the sampling region, the distance between the light sources and the solid surface is controlled so that a linearly partitioned sampling region  10  can be formed when the central point  132   a  and the auxiliary point  133   a  overlap each other. 
         [0091]    Specifically, as shown in  FIGS. 9 and 10 , in the step (S 100 ) of projecting the light source, the main light source projects a partitioning image having a “         ” or “         ” shape having a central point  132   a  through a diffraction lens that emits diffused light, and the auxiliary light source projects a slant auxiliary point  133   a  that progresses toward the central point  132   a.  In the step (S 200 ) of forming the sampling region, the levels of the images projected from the main light source and the auxiliary light source are controlled, so that a partitioned sampling region  10  having any one shape of “         ” and “         ” when the central point  132   a  and the auxiliary point  133   a  overlap each other. 
         [0092]    Herein, the above central point and auxiliary point may have various shapes such as “•” or “x”. 
         [0093]    In addition, using a single laser-type light source having straightness, an image having a predetermined shape is projected from the light source, so that a linearly partitioned sampling region  10  can be formed. Specifically, as shown in  FIGS. 11 and 12 , an image having any one shape of “         ” and “         ” is projected from the light source, so that a linearly partitioned sampling region  10  can be formed. 
         [0094]    Meanwhile, in the step (S 200 ) of forming the sampling region, the partitioned sampling region  10  formed by projecting the image from the light source most preferably has an area of 100 cm 2 . 
         [0095]    Hereinafter, an apparatus for partitioning a sampling region on a solid surface in a contactless manner, which is used in the above-described method for collecting a microbial sample from a solid surface using a contactless partitioning system, will be described in detail with reference to the accompanying drawings. 
         [0096]      FIG. 13  is a perspective view showing a first embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner, and  FIG. 14  is a cross-sectional view showing a first embodiment of the inventive apparatus for partitioning a sampling region on a solid surface in a contactless manner. 
         [0097]    As shown in  FIGS. 13 and 14 , the inventive apparatus  1  for partitioning a sampling region on a solid surface in a contactless manner comprises a small-sized cylindrical body  100  which is easily grasped by the operator&#39;s hand. 
         [0098]    Herein, a battery  110  for supplying power is included in the body so that it can be used as a power source. In addition, an ON/OFF operating switch which is electrically connected to the battery  110  is provided at the top of the body  100  so as to be exposed to the outside. 
         [0099]    Also, a light source  130  which faces downward is provided at the bottom of the body  100  and is switched ON/OFF by the operation of the operating switch  120 . 
         [0100]    Further, a partitioning means  140  for partitioning a figure-shaped sampling region  10  is provided below the light source  130  and is configured such that light from the light source  130  passes through the partitioning means  140  so that an image corresponding to the sampling region  10  is projected onto the solid surface P. 
         [0101]    Herein, the light source includes a lamp-type light source that emits diffused light, and the partitioning means  140  includes a colored filter which is generally impermeable to light, provided that a transparent, light-permeable partitioning region is formed in the center of the colored filter  141  as shown in  FIGS. 17 and 18 , so that light from the light source  130  passes through the partitioning portion  142  of the colored filter  141  to project the figure-shaped sampling region  10  on the solid surface P. 
         [0102]    Meanwhile, the sampling region  10  as described above may have various shapes. In the present invention, a square shape and a circular shape are applied. Preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. 
         [0103]    Each of the lamp-type light source and the colored filter  141  is divided onto two parts. As shown in  FIG. 17(   a ), the partitioning portions  142  of the colored filters  141  and  141 ′ include two opposite linear partitioning  FIGS. 142   a  and  142   a ′ having shapes of “         ” and “         ” or “         ” and “         ”, so that light having passed through each of the colored filters  141  and  141 ′ forms a linearly partitioned sampling region  10  on the solid surface  10 . 
         [0104]    Specifically, when the sampling region  10  is partitioned on the solid surface P using two lamp-type light sources  131  and  131 ′ and colored filters  141  and  141 ′ as described above, the sampling region  10  having a “         ” or “         ” shape as shown in  FIG. 2  or  3  with respect to the sample collection method is partitioned. 
         [0105]    In addition, as shown in  FIG. 15 , four lamp-type light sources  131  and four colored filters  141  are used. In this case, as shown in  FIG. 17(   b ), the partitioning portions  142  of the colored filters  141  and  141 ′ include four radially opposite linear partitioning FIGS.  141  and  141 ′ having shapes of “         ”, “         ”, “         ” and “         ” or “         ”, “         ”, “         ” or “         ”, so that light having passed through each of the colored filters  141  and  141 ′ forms a linearly partitioned sampling region  10  on the solid surface P. 
         [0106]    Specifically, when the sampling region  10  is partitioned on the solid surface P using four lamp-type light sources  131  and  131 ′ and four colored filters  141  and  141 ′ as described above, the sampling region having a “         ” or “         ” shape as shown in  FIG. 4  or  5  with respect to the sample collection method is partitioned. 
         [0107]    Moreover, as shown in  FIG. 16 , one lamp-type light source  131  and one colored filter are used. In this case, as shown in  FIG. 17(   c ), the partitioning portion  142  of the colored filter  141  includes a linear partitioning  FIG. 142   a  having a shape of “         ” or “         ”, so that light having passed through the colored filter  141  forms a linearly partitioned sampling region  10  on the solid surface P. 
         [0108]    Specifically, when the sampling region  10  is partitioned on the colored filter using one lamp-type light source  131  and one colored filter  141  as described above, the sampling region  10  having a shape of “         ” or “         ” as shown in  FIG. 6  or  7  with respect to the sample collection method is partitioned. 
         [0109]    Meanwhile, as shown in  FIG. 18 , the transparent partitioning portions  142  of the colored filters  141  and  141 ′ may include partitioning surfaces  142   b  and  142   b ′. In this case, light having passed through the colored filters  141  and  141 ′ forms a surface-shaped partitioning region  10  on the solid surface P. Specifically, the sampling region  10  is partitioned on the solid surface P using the colored filters  141  and  142  having the partitioning surfaces  142   b  and  142   b ′ as described above, the sampling region  10  having a shape of “         ” or “         ” as shown in  FIG. 8  with respect to the sample collection method is partitioned. 
         [0110]    In addition, as shown in  FIG. 19 , the light source  130  of the body  100  of the partitioning apparatus may comprise the laser-type main light source  132  having straightness, and the partitioning means  140  may comprise the diffraction lens  145  having the property of enlarging an image, and the laser-type auxiliary light source  133  having straightness may further be provided at one side of the body  100  of the partitioning apparatus so as to be slanted toward the main light source  132 . In this case, the main light source  132  is configured to irradiate light along the partitioning line  142   a  and the central point  132   a  of the region partitioned by the partitioning line  142   a,  and the auxiliary light source  133  is configured to irradiate light onto the auxiliary point  133   a  that progresses toward the central point  132   a.  The distance between the body  100  and the solid surface P is controlled so that a figure-shaped sampling region  10  is partitioned on the solid surface P when the central point  312   a  and the auxiliary point  133   a  overlap each other as shown in  FIG. 20 . 
         [0111]    Specifically, when the sampling region  10  is to be partitioned on the solid surface P using the main light source  132  and the auxiliary light source  133  as described above, the central point  132   a  of the main light source  132  overlaps with the auxiliary point  133   a  of the auxiliary light source  133  as shown in  FIG. 9  or  10  with respect to the sample collection method, and thus the sampling region  10  having a shape of “         ” or “         ” is partitioned on the solid surface. 
         [0112]    In addition, as shown in  FIG. 21 , the battery  110  for supplying power is provided in the body  100 , and the ON/OFF operating switch  120  is provided at the top of the body  100 , and a laser-type light source  135  having straightness is provided at the bottom of the body  100 , wherein the light source  135  is configured to irradiate light along a partitioning line  142   a  as shown in  FIG. 22  so as to project an image corresponding to the sampling region  10  onto the solid surface  10 . 
         [0113]    Specifically, when the sampling region  10  is to be partitioned on the solid surface P using the light source  135  as described above, the sampling region  10  having the same size as the partitioning line  142   a  and a shape of “         ” or “         ” as shown in  FIG. 11  or  12  with respect to the sample collection method is partitioned. 
         [0114]    Meanwhile, the figure-shaped sampling region  10  projected on the solid surface P by the projection of the light source  130  preferably has an area of 100 cm 2 . 
         [0115]    When the above-described method for collecting a microbial sample from the solid surface using a contactless partitioning system and the above-described apparatus for partitioning a sampling region on a solid surface are used, the sampling region can be partitioned in a convenient and rapid manner, and the contamination of the solid surface can be fundamentally prevented, and thus the accuracy of sampling operations such as microbial collection can be further increased. 
         [0116]    Furthermore, the apparatus can be reused, and thus the cost of a sampling operation can be significantly reduced.