Abstract:
Disclosed is the molecular cloning of HTLV-III, the adult leukemia and acquired immune deficiency syndrome (AIDS) virus. Clone BH10 contains a 9.0 Kb viral insert constituting the entire HTLV-III genome. Clones BH8 and BH5 contain viral inserts of 5.5 Kb and 3.5 Kb, respectively. These clones are suitable for the development of diagnostic and therapeutic measures for AIDS, as well as use as probes for the detection of AIDS. By scientific convention, HTLV-III, referred to herein also as HIV, has been renamed as HIV-1.

Description:
REFERENCE TO RELATED APPLICATIONS 
     This application, Ser. No. 08/385,231, filed Feb. 8, 1995, is a file wrapper continuation of patent application Ser. No. 07/832,603, filed Feb. 12, 1992, now abandoned, which is a file wrapper continuation of patent application Ser. No. 07/160,827, filed Feb. 26, 1988, now abandoned, which is (i) a continuation-in-part of patent application Ser. No. 07/033,891, filed Apr. 3, 1987, now abandoned, which is a continuation of patent application Ser. No. 06/643,306, filed Aug. 22, 1984, now abandoned, (ii) a continuation-in-part of patent application Ser. No. 06/693,866, filed Jan. 23, 1985, pending, which is a continuation-in-part of patent application Ser. No. 06/659,339, filed Oct. 10, 1984, now abandoned, which is a continuation-in-part of patent application Ser. No. 06/643,306, filed Aug. 22, 1984, now abandoned, and (iii) a continuation-in-part of patent application Ser. No. 06/813,069, filed Dec. 24, 1985, now abandoned, the disclosures of which are incorporated herein by reference in their entirety for all purposes. 
    
    
     TERMINOLOGY 
     The causative agent of acquired immune deficiency syndrome (AIDS) has been known as human T-lymphotropic virus type III (HTLV-III) and as human immunodeficiency virus (HIV). The virus, in accord with the newer practice, will be called HIV except in some instances where a deposit relating to the organism has been made using the earlier terminology. 
     BRIEF DESCRIPTION OF THE INVENTION 
     The cultivation of viruses using molecular clones provides a dependable source of virus for study of the natural virus and for preparation of diagnostic and immunogenic products of the virus. The isolation of virus believed to be the causative agent of AIDS was reported by Barre-Sinoussi, et al. in  Science , Vol. 220, at pages 868-870 (1983). However, no reproduction of the virus in an immortalized cell line is disclosed in that publication. HIV is highly cytopathic to the cells which it infects in nature. This is one characteristic which differentiates HIV from related retroviruses such as HTLV-I and HTLV-II. HIV is further characterized by variation of its genome in nature. Gallo, et al. discovered cell lines useful for continuous production of the virus. The use of such cell lines which are CD-4 positive cells was disclosed in U.S. patent application Ser. No. 06/652,599, which issued as U.S. Pat. No. 4,652,599. The disclosure of that patent is incorporated herein by reference. The disclosure herein provides means for producing clones of virus which are grown in the immortalized cell lines. 
     The infectious clones of the inventions are useful for producing specific viral proteins in both eukaryotic and prokaryotic systems for use in diagnostic evaluation and for vaccine development. The infectious clones also provide a source of homogeneous viral particles for use in evaluation of vaccines. 
     While HXB2 and HXB3 were shown to be non-infectious or only mildly infectious, infectious clones which have been derived therefrom are disclosed. Transfection of the derivative clones into bacteria provides a means for amplifications of the genome of these clones. 
     It is the object of this invention to provide a reliable source of HIV, viral particles, proteins, and antibodies by preparation of clones containing essentially the entire genome of the HIV. The virus or viral fragments produced in immortalized cell lines are useful as probes to detect HIV viral sequences in HIV strains isolated from patients. By use of such probes the variant strains of HIV can be studied as a means of determining source of the disease in an individual. Such determination of source is vital in evaluating means of transmission of this disease. 
     It is a further object of the invention to provide reliable sources of viral products for use as immunogens and diagnostic agents. 
     BACKGROUND OF THE INVENTION 
     The characterization of HIV as the causative agent of AIDS by Barre-Sinoussi, et al. [ Science , Vol. 220 (1983)] did not provide enablement for producing the virus in vitro. However, it was discovered by workers in this laboratory that the causative agent of AIDS could be grown in immortalized CD-4 positive cell lines to provide a reliable source of the virus and viral products. The use of these products as diagnostic tools is disclosed in U.S. Pat. No. 4,520,113, which is incorporated herein by reference. 
     A method of cloning human T-cell leukemia-lymphoma virus (HTLV), a transforming virus which lacks both the variability and cytopathic properties of HIV, is taught in Manzari, et al., [Proc. Natl. Acad. Sci., Vol. 80, pages 1574-1577 (1983)]. There is no teaching of how to clone a highly cytopathic virus of such diverse genomic structure as the HIV. To obtain a virus for cloning, it was necessary to have an infected, immortalized cell line from which to extract the virus. U.S. Pat. No. 4,652,599 to Gallo, et al. teaches such cell lines. 
    
    
     
       DESCRIPTION OF THE FIGURES 
         FIG. 1  is a Southern blot analysis of unintegrated DNA of HIV. No viral sequences could be detected in the undigested DNA after 4 hours. However, a major species of viral DNA of approximately 10 kb length was present in the 10, 15, 24 and 48 hour harvest representing the linear unintegrated form of the virus. A representative Southern blot of the 15 hour harvest digested with several restriction enzymes is shown in this figure. Methods: 8×10 8  fresh uninfected H9 cells were infected with concentrated supernatant from cell line H9/HTLV-III (H9/HIV) containing 4×10 11  particles of HIV. Infected cells were divided into five Roller bottles and harvested after 4, 10, 15, 24 and 48 hours. Low molecular weight DNA was prepared using the Hirt fractionation procedure and 30 g of undigested and digested DNA were separated on a 0.8% agarose gel, transferred to nitrocellulose paper and hybridized to a HIV cDNA probe for 24 hours at 37° C. in 1×SSC, 40% formamide and 10% Dextran sulfate. cDNA was synthesized from poly(A) selected RNA prepared from doubly banded HIV virus in the presence of oligo(dT) primers. Filters were washed at 1×SSC at 65° C. 
         FIG. 2  is a restriction endonuclease map of two closely related HIV variants cloned from unintegrated viral DNA. Three recombinant clones (λBH10, λBH5 and λBH8) were analyzed and their inserts (9 Kb, 5.5 Kb and 3.5 Kb, respectively) were mapped with the indicated enzymes. They represent two variant forms of HIV differing in three enzyme sites which are depicted in bold letters and by an asterisk. As SstI cuts the LTR of the HIV the three clones represent two full-length genomes with one LTR. A schematic map of this viral genome is shown at the bottom of the figure, although the total length of the LTR is approximate. 
         FIG. 3  demonstrates HIV viral sequences in the infected cell line H9/HIV. Both variant forms of HIV were detected as integrated provirus as well as unintegrated viral DNA in the infected cell line. However, no viral sequences were found in uninfected H9 cells, uninfected HT cells nor in normal human thymus (NT). 
         FIG. 4  shows a sequence homology of HIV to related retroviral members of the HTLV family. A schematic restriction map of HTLV-I, HTLV-Ib and HTLV-II is drawn below indicating the length and the location of the generated fragments in respect to the corresponding genomic regions. 
         FIG. 5  represents the entire nucleotide sequence of the molecular clone BH5 (ATCC #40126), which is approximately one half of a cloned genomic sequence for an HIV strain on deposit with the American Type Culture Collection. 
         FIG. 6  represents the entire nucleotide sequence of the molecular clone BH8 (ATCC #40127), which is approximately the second half of the genomic sequence for the HIV strain noted in  FIG. 5 . 
         FIG. 7  represents the entire nucleotide sequence of the molecular clone BH10 (ATCC #40125), which is approximately the entire cloned genomic sequence of an HIV strain, different from the strain noted in  FIGS. 5 and 6 , on deposit with the American Type Culture Collection. 
         FIG. 8  shows restriction endonuclease maps of four closely related clones of HIV. λHXB-2 and λHXB-3 represent full-length integrated proviral forms of HIV obtained from the λ phage library of H9/HIV DNA (Example 3). These clones contain the complete provirus (thin lines) including two LTR regions plus flanking cellular sequences (heavy lines). The LTR regions are known to contain the three restriction enzyme sites Bgl II, Sst I, and Hind III, as shown, but their overall lengths are estimated. Clones λBH-10 and λBH-5/λBH-8 are shown here for comparison with λHXB-2 and λHXB-3 and with Southern blots of genomic DNA from other HTLV-III containing cells. It should be noted that λBH-5/λBH-8 consists of two separate clones Sst I fragments (λBH-5 and λBH-8) which together constitute one HIV genomic equivalent but which are not necessarily derived from the same viral molecule. Also, because λBH-10 and λBH-5 were cloned with the restriction enzyme Sst I, they lack 5′ LTR sequences as shown. Other differences in the restriction maps between these HIV clones are indicated by bold letters and asterisks, with λBH-10 being used as a reference. 
         FIG. 9  shows the construction of a plasmid containing sequences of the HIV genome. A 12.7-kb XbaI fragment derived from HXB-2, a molecular clone containing about 10 kb of HIV proviral sequences, was inserted into the XbaI site in the polylinker of plasmid pSP62 to produce the plasmid clone pHXB-2D. This plasmid construct was then transfected into DH-1 bacteria and used in protoplast fusion experiments. The thin horizontal line represents HIV and the solid boxes represent flanking cellular sequences. 
     
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     Clones are prepared using both unintegrated DNA and integrated DNA proviral DNA. The clones of integrated DNA and unintegrated DNA are similar, but are distinguishable by differences in several restriction cleavage sites. From  FIG. 8 , it is shown λBH-10 and λBH-5/λBH-8 are incomplete viral clones which lack a short SstI-SstI segment of approximately 190 base pairs in the 5′ LTR-leader sequence as a consequence of use of Sst I in their cloning. The λHXB-2 and λHXB-3 clones contain full-length integrated provirus [˜10 kilobases (kb)] with cellular flanking sequences. 
     Plasmids are constructed using λHXB-2 to produce pHXB-2D A 12.7 kb XbaI fragment derived from pHXB-2D was inserted into the XbaI site in the polylinker of plasmid pSP62 to provide a plasmid suitable for transfection into the DH-1 bacteria. 
     Preparation of Clones λBH10, λBH5, and λBH8 
     Example 1 
     Concentrated virus from the H9/HTLV-III cell line as used to infect fresh uninfected H9 cells at a multiplicity of 50 viral particles per cell and cultures were collected after 4, 10, 15, 24 and 48 hours. Extrachromosomal DNA was extracted according to the procedure of Hirt [Hirt, R.,  J. Molec. Biol.  26: 365-367 (1967)] and assayed for its content of unintegrated viral DNA using HIV cDNA as a probe. The synthesis of this cDNA was primed with oligo(dT) and reverse-transcribed from poly(A)-containing RNA of virions that had been banded twice on sucrose density gradients [Arya, et al.,  Science  225: 927-930 (1984)]. Unintegrated linear viral DNA was first detected after 10 h and was also present at the subsequent time points. ( FIG. 1  shows a Southern blot of the 15-h sampling.) A band of ˜10 kilobases (kb) in the undigested DNA represents the linear form of unintegrated HIV. No closed or nicked circular DNA could be detected at 10, 15 or 24 hours, but both forms were evident in small amounts at 48 hours (data not shown). The viral genome was not cleaved by XbaI, whereas SstI generated three predominant bands of 9, 5.5 and 3.5 kb ( FIG. 1 ). These bands represent the genomes of two forms of HIV, both cut by SstI in or near the long terminal repeat (LTR), and one having an additional SstI site in the middle of its genome. The other enzymes generate a more complex pattern of restriction fragments. 
       FIG. 2  shows the restriction map of three clones, designated λBH10, λBH5 and λBH8, which correspond in size to the three SstI fragments shown in  FIG. 1 . Comparison of these maps suggests that λBH5 plus λBH8 constitute one HIV genome, and BH10 another. The two viral forms differ in 3 of 21 mapped enzymes sites, including the internal SstI site. As expected, the phage inserts of λBH5 and λBH8 hybridize in high-stringency conditions (T m -25° C.) to λBH10 but not to each other, as analyzed by Southern blot hybridization and electron microscopic hetero-duplex analysis (data not shown). To determine the orientation of the three clones, we used as a probe a cDNA clone (C15) containing U3 and R sequences. C15 hybridized strongly to the 0.5 kb BglII fragment of λBH10 and λBH8, orienting this side 3′. Assuming that SstI cuts only once in the vicinity of the HIV LTR, the clones λBH10 and λBH5/λBH8 represent two complete genomic equivalents of the linear unintegrated form of HIV that vary in three restriction enzyme sites. 
     Methods: Low molecular weight DNA combined from the 15 and 24 hour harvest was fractionated on a 10-40% sucrose gradient. Aliquots of the fractions were electrophoresed on a 0.5% agarose gel, transferred to nitrocellulose paper and hybridized to HIV cDNA under conditions described in  FIG. 1 . Fractions which contained the unintegrated linear HIV genome shown by hybridization were pooled, the DNA was subsequently digested with SstI and ligated to phosphatase treated SstI arms of λgtWes. λB. After in vitro packaging, recombinant phages were screened for viral sequences with HIV cDNA. 
     Example 2 
     The presence of two variant forms of HIV in the original cell line was demonstrated by hybridizing the radiolabelled insert of λBH10 to a Southern blot of H9/HIV genomic DNA digested with several restriction enzymes ( FIG. 3 ); both forms were detected using SstI, which generated the expected three bands of 9, 5.5 and 3.5 kb. XbaI, which does not cut the provirus, generated a high-molecular weight smear representing polyclonal integration of the provirus, plus a band of ˜10 kb. This 10-kb band was also detected in undigested H9/HIV DNA (not shown), indicating that it represents unintegrated viral DNA. The presence of unintegrated viral DNA also explains the 4- and 4.5-kb EcoRI fragments seen in both the Hirt and total cellular DNA preparations ( FIGS. 1, 3 ). Both BglII and HindIII cut within the LTR and generate the expected internal bands. Several faint bands in addition to the expected internal bands generated by HindIII digestion, represent either defective proviruses or other variant forms of HIV present in low copy number. 
     Method: 10 μg of high molecular weight DNA were digested with restriction enzymes as indicated and hybridized to nick translated phage insert from BH10 under the same conditions as described in  FIG. 1 . 
     For comparison, sub-clones of full length genomes of a prototype HTLV-I, HTLV-Ib, HTLV and GaLV (Seato strain) were digested with the following enzymes, PstI plus SstI (HTLV-I and HTLV-Ib), BamHI plus SmaI (HTLV-II) and Hind III plus SmaI plus XhoI (GaLV). Four replicate filters were prepared and hybridized for 36 hours under low stringency (8×SSC, 20% formamide, 10% Dextran sulfate at 37° C.) to nick translated insert of λBH10. Filters were then washed in 1×SSC at different temperatures, 22° C. (Tm−70° C.) filter 1, 37° C. (Tm−56° C.) filter 2, 50° C. (Tm−42° C.) filter 3 and 65° C. (Tm−28° C.). 
       FIG. 4  shows a sequence homology between HIV and other related retroviruses. Hybridization of HIV with the related HTLV family could be detected where no hybridization to GaLV was seen. 
     Preparation of Clones Containing Integrated Proviral DNA 
     Example 3 
     The HIV is used to infect H9 cells in accord with the method of Example 1. Preliminary analyses of Southern digests of H9/HIV DNA reveals that the virus is present in this cell line both as unintegrated DNA and as proviral DNA integrated into the cellular genome at multiple different sites. Since the HIV provirus lacks Xba I restriction sites, a genomic library was constructed by using Xba I-digested H9/HIV DNA, and this was screened with an HIV cDNA probe to obtain molecular clones of full-length integrated provirus with flanking cellular sequences. Fourteen such clones were obtained from an enriched library of 10 6  recombinant phage, and two of these were plaque-purified and characterized. (See  FIG. 8 .) 
     To show that the restriction enzyme cleavage sites depicted in  FIG. 8  for clones λHXB-2 and λHXB-3 are actually present in the viral DNA of HIV-infected H9 cells, DNA was digested from the H9/HIV cell line with various restriction enzymes and analyzed it by the Southern blot technique. The restriction fragments for Sst I, Eco RI, Hind III, Pst I, Bam H1, and BglII predicted from the restriction maps of λHXB-2 and λHXB-3 ( FIG. 8 ) are shown to be present in the Southern blots of HIV infected cellular DNA. 
     Example 4 
     To determine whether the HIV genome contains sequences homologous to normal human DNA, the viral insert of λHXB-2 (5.5 kb and 3.5 kb Sst I-Sst I fragments) was isolated, nick translated, and used to probe HIV-infected and uninfected cellular DNA. Under standard conditions of hybridization [washing conditions: 1×SSC (standard saline citrate), 65° C.; annealing temperature T m −27 C], this probe hybridizes to DNA from H9/HIV cells as well as other HIV-infected cells, but not to DNA from uninfected H9 cells, uninfected HT cells (the parent cell line from which H9 as cloned), or normal human tissues (data not shown). This finding is in agreement with previous results in which the unintegrated (replicative intermediate) form of HIV was used as probe and demonstrates that HIV, like HTLV-1 and HTLV-II, is an exogenous retrovirus lacking nucleic acid sequences derived from human DNA. 
     Example 5 
     A 12.7 kb XbaI fragment derived from λHXB-2 is inserted into the polylinker of plasmid pSP62 to produce plasmid clone pHXB-2D ( FIG. 9 ). The pHXB-2D is transfected into DH-1 bacteria for use in protoplast fusion experiments. 
     Example 6 
     Kinetics of cell growth and reverse transcriptase activity in cord blood mononuclear cell cultures following protoplast fusion: Mononuclear cells were prepared from cord blood samples using Ficoll Triosil and cultured for 5 days in media containing PHA. These cells were then fused with bacterial protoplasts carrying the plasmid pHXB-2D, pSV2neo or pCH-1gpt and maintained in culture at a density of 5×10 5  cells ml −1  by addition of RPMI-1640 medium containing 20% fetal calf serum, 10% T-cell growth factor (inter-leukin-2) and antibiotics. Three parallel fusions using cells from different individuals were established for each plasmid. Spent medium removed from two cultures at 5, 11, 14 and 18 days after fusion was concentrated 10-fold and assayed for the presence of reverse transcriptase using standard techniques. The activity detected in each of the culture supernatants is expressed as the amount of  3 H-labeled deoxyribonucleotide monophosphate ( 3 H-dTMP) incorporated (in pmol per 0.3-ml sample) using dT 15 ·(rA) n  as the template primer. 
     The growth of all cultures was comparable for the first 14 days after protoplast fusion. By day 18, however, the number of viable cells in cultures transfected with pHXB-2D had fallen dramatically: there was a 10-fold and a 100-fold reduction between days 18 and 21 and 18 and 32, respectively. Cultures transfected with either pSV2neo or pCH-1gpt showed only a 4-5-fold reduction over the same time period. When supernatant from the cultures was assayed for the presence of reverse transcriptase, activity was detected exclusively in cultures transfected with pHXB-2D. These data suggest that replicating virus was present in cultures 11-18 days after fusion with pHXB-2D protoplasts. 
     Example 7 
     Expression of the HIV gag-related proteins p15 and p24 by transfected cells was demonstrated using specific monoclonal antibodies. Maximum expression was observed 18 days after transfection, when 4-11% and 5-9% of cells were reactive with antibody to p15 and p24, respectively. Virus particles were detected by electron microscopy in all cultures 14-18 days after transfection with pHXB-2D. The particles contained condensed, truncated cores, which are characteristic of HIV particles. 
     Example 8 
     In time-course experiments, DNA isolated from a single culture 6, 11, 14, 18 and 31 days after transfection with pHXB-2D, was digested with BamHI and analyzed for HIV sequences. Six days after transfection, an 8.6-kb DNA fragment was detected as a faint band; 18 days after transfection it was possible to detect a 1.5-kb DNA fragment in addition to the 8.6-kb fragment. The total amount of unintegrated virus in the cultures appeared to increase, as suggested by the increase in intensity of these bands with time; this is evidence that cells originally transfected with pHXB-2D are able to produce fully infectious virus which is then transmitted within the culture. 
     No HIV viral sequences were detected 31 days after transfection; at this point the culture may have contained only cells which failed to be infected by HIV. This result is again consistent with the transfected DNA exerting a cytopathic effect on T cells. The finding that, at any stage, only a minor population of the transfected cells are apparently infected by the virus (&lt;15% express viral proteins) suggests that the cytopathic effects may not result solely from direct viral infection and that secreted factors and/or other cell-to-cell interactions may play a part in the cytopathic phenomenon. 
     The biological materials relating to the invention have been deposited at the American Type Culture Collection, Rockville, Md., under the following accession numbers: 
     
       
         
               
               
               
             
           
               
                   
                   
               
             
             
               
                   
                 λBH-10 
                 40125 (FIG. 7)  
               
               
                   
                 λBH-5 
                 40126 (FIG. 5 ) 
               
               
                   
                 λBH-8 
                 40127 (FIG. 6) 
               
               
                   
                 λ-HXB 2   
                 40231 
               
               
                   
                 λ-HXB 3   
                 40232 
               
               
                   
                 pHXB3 
                 67081 
               
               
                   
                 pHXB-2D 
                 67082 
               
               
                   
                 X10-1 ( E   coli  DH-1) 
                 67083 
               
               
                   
                   
               
             
          
         
       
     
     Upon issuance of a patent on the present invention, this deposit will continue to be viably maintained for 30 years and made available to the public without restriction, of course, consistent with the provisions of the law. 
     Examples of useful products are now described: 
     
         
         1. Viral particles and proteins may be extracted from both supernatants and whole cells. 
         2. Supernatant material may be purified for use in test kits for immunoblotting and immunoadsorbent tests. 
         3. Monoclonal antibodies may be produced which react against HIV antigens. 
         4. The antigens may be used as immunogens in vaccine development. 
       
    
     Both antibodies and antigens can be used in diagnostic kits. Both antibodies and antigens can be provided as compositions. Particularly preferred compositions of matter are solid supports having antigens of the invention adhering thereto for use in identifying antibodies to HIV proteins for use in Enzyme-linked-immunoabsorbent (ELISA) assays. 
     It is understood that the examples and embodiments described herein are for illustration purposes. Examples are not intended to be viewed as limitations since many obvious modifications are within the scope of one skilled in the art. 
     
       
         
               
             
               
               
             
           
               
                 TABLE I 
               
             
             
               
                   
               
               
                 CLAIMS SUPPORT CHART 
               
             
          
           
               
                   
                 Support in U.S. patent application 06/643,306 
               
               
                 Claim 
                 filed on Aug. 22, 1984 
               
               
                   
               
               
                 61. (new) A method for detecting the presence of a polynucleotide 
                 page 1, lines 28-34. discusses the use of cDNA clones of the invention  
               
               
                 comprising a human immunodeficiency virus (HIV) nucleotide sequence in a 
                 to distinguish HTLV III from HTLV I and II. 
               
               
                 nucleic acid sample obtained from a physiological sample, which method 
                 p. 3 line 25 to p. 4 line 8 discuss regions of homology and regions of  
               
               
                 comprises the steps of: 
                 variability between HTLV III, and HTLV I and II, that can be exploited  
               
               
                 (a) combining said nucleic acid sample with a single-stranded 
                 in distinguishing between the different viruses. 
               
               
                 nucleic acid probe comprising a sequence of at least about 18 contiguous bases 
                 p. 5, lines 12-14 discusses production of a cDNA library for use in  
               
               
                 selected from one of the nucleotide sequences shown in FIGS. 5, 6 or 7 and 
                 analyzing the HIV genome. 
               
               
                 complementary to said HIV genomic sequence comprised in said polynucleotide, 
                 p. 5 line 29 - p. 6 line 10 provides support for making probes from HIV  
               
               
                 said probe not forming a duplex with HTLV-I and -II nucleic acid sequences under 
                 mRNA. 
               
               
                 conditions of stringency for hybridization under which said probe forms a duplex 
                 p. 6, lines 23-26 discusses using probes to assay viral DNA 
               
               
                 with said polynucleotide; and 
                 p. 7, lines 18-30 discuss using an λ phage clone in Southern analysis of 
               
               
                 (b) determining duplex formation between said probe and nucleic 
                 restriction fragments from HIV DNA. 
               
               
                 acid present in said sample. 
                 Statement of Deposit, p. 6. 
               
               
                   
                 p. 1 discussion relating to detection of HIV in human sera. 
               
               
                   
                 BH10 contains an 18 base BglII-SstI restriction fragment. 
               
               
                   
                 FIG. 4 shows that only a fraction of the HTLV-I and -II genomes  
               
               
                   
                 hybridize to HTLV-III. 
               
               
                   
                 Example 2, p. 8 discusses the use of stringency washes to distinguish  
               
               
                   
                 homology between HIV, HTLV I and HTLV II. 
               
               
                 62. (new) The method of claim 61 wherein the probe sequence is 
                 p. 3 contains a discussion relating to the presence of gag, pol and env in  
               
               
                 complementary to a sequence which is part of the gag, pol or env open reading 
                 HTLV-III. 
               
               
                 frame. 
                   
               
               
                 63. (new) The method of claim 62 wherein the probe sequence is 
                   
               
               
                 complementary to a sequence which is part of the gag open reading frame. 
                   
               
               
                 64. (new) The method of claim 62 wherein the probe is complementary to a 
                   
               
               
                 sequence which is part of the pol open reading frame. 
                   
               
               
                 65. (new) The method of claim 61 wherein the probe comprises RNA. 
                 p. 5 discussion of the use of RNA as probe/probe template. 
               
               
                 67. (new) The method of claim 62 wherein the probe comprises RNA. 
                   
               
               
                 66. (new) The method of claim 61 wherein the probe comprises DNA. 
                 Support noted for claim 1 refers to DNA probes 
               
               
                 68. (new) The method of claim 62 wherein the probe comprises DNA. 
                   
               
               
                 69. (new) A method comprising the steps of: 
                 Support noted for claim 61 also applies here. 
               
               
                 (a) providing a sample suspected of containing a polynucleotide; 
                 clone BH5 contains a HindIII-XbaI fragment that is 103 bases in  
               
               
                 (b) providing a single-stranded nucleic acid of 18-103 bases 
                 length. 
               
               
                 comprising a sequence of bases of at least 18 contiguous bases selected from the 
                 p. 3 contains a discussion relating to the presence of gag, pol and env in  
               
               
                 gag, env, or pol open reading frames of FIG. 5, 6 or 7 or the complement thereof; 
                 HTLV-III. 
               
               
                 and 
                   
               
               
                 (c) combining said sample and said single-stranded nucleic acid 
                   
               
               
                 under hybridization conditions that (i) permit duplex formation between said single-  
                   
               
               
                 stranded nucleic acid and either strand of viral DNA from a lambda bacteriophage 
                   
               
               
                 selected from the group consisting of ATCC Accession no. 40143 and 40144, but 
                   
               
               
                 (ii) do not permit duplex formation with either HTLV-I or HTLV-II genomic 
                   
               
               
                 sequences. 
                   
               
               
                 70. (new) A method comprising the steps of: 
                   
               
               
                 (a) providing a sample suspected of containing a polynucleotide; 
                   
               
               
                 (b) providing a single-stranded nucleic acid of 32-103 bases 
                   
               
               
                 comprising a sequence of bases of at least 32 contiguous bases selected from the 
                   
               
               
                 gag, env, or pol open reading frames of FIG. 5, 6 or 7 or the complement thereof; 
                   
               
               
                 and 
                   
               
               
                 (c) combining said sample and said single-stranded nucleic acid 
                   
               
               
                 under hybridization conditions that (i) permit duplex formation between said single- 
                   
               
               
                 stranded nucleic acid and either strand of viral DNA from a lambda bacteriophage 
                   
               
               
                 selected from the group consisting of ATCC Accession no. 40143 and 40144, but 
                   
               
               
                 (ii) do not permit duplex formation with either HTLV-I or HTLV-II genomic 
                   
               
               
                 sequences. 
                   
               
               
                 71. (new) The method of claim 69 or 70 wherein contiguous bases are from 
                 p. 3 contains a discussion relating to the presence of gag, pol and env in  
               
               
                 the gag open reading frame or the complement thereof. 
                 HTLV-III. 
               
               
                 72. (new) The method of claim 69 or 70 wherein said contiguous bases are 
                   
               
               
                 from the env open reading frame or the complement thereof. 
                   
               
               
                 73. (new) The method of claim 69 or 70 wherein said contiguous bases are 
                   
               
               
                 from the pol open reading frame or the complement thereof. 
                   
               
               
                 74. (new) The method of claim 61, 69 or 70 wherein said single-stranded 
                 SstI pg. 2 and FIG. 2; 
               
               
                 nucleic acid comprises DNA and wherein said contiguous bases are within a 
                 HindIII pg. 4, and FIG. 2; 
               
               
                 restriction fragment produced by cleavage of the nucleic acid presented in FIG. 5, 
                 PstI pg. 2 and FIG. 2 
               
               
                 6 or 7 using one or more restriction enzymes selected from the group consisting of 
                 Bgl II pg. 7 and FIG. 2; 
               
               
                 SstI, HindIII, PstI, Bgl II, Kpn I, EcoRI, BamHI, HpaI, XhoI, XbaI and SmaI. 
                 Kpn I FIG. 2; 
               
               
                   
                 EcoRI Page 7 and FIG. 2; 
               
               
                   
                 BamHI Page 2 and FIG. 2; 
               
               
                   
                 HpaI Page 2 and FIG. 2; 
               
               
                   
                 XhoI Page 2 and FIG. 2; 
               
               
                   
                 XbaI Page 7 and FIG. 3; and 
               
               
                   
                 SmaI FIG. 2. 
               
               
                 75. (new) The method of claim 74 wherein the single-stranded nucleic acid 
                 p. 9, lines 28-32 discusses the use of λBH10 and restriction fragments  
               
               
                 probe comprises one of the nucleotide sequences selected from the group consisting 
                 to analyze the HIV genome. 
               
               
                 of: [Restriction Fragments supported in Wong-Staal specification.] 
                 p. 6, lines 23-26 discusses using cDNA to detect viral DNA 
               
               
                 76. (new) The method of claim 75, wherein the single-stranded nucleic acid 
                 p. 7, lines 18-30 and p. 9 lines 15-26 discuss using an λ phage clone in  
               
               
                 is 5′-CTTTAAGACCAATGACTTACAAGGCAGCTGTA -3′. 
                 Southern analysis of restriction fragments from HIV DNA. 
               
               
                   
                 32 nucleotide restriction fragment present in BH8. 
               
               
                   
                 Nucleotide sequence of claim 76 nucleic acid is a 32 nucleotide KpnI- 
               
               
                   
                 BglII restriction fragment from clone BH8 
               
               
                 77. (new) A method for detecting the presence of a polynucleotide 
                 Support cited for claim 1 is applicable here. 
               
               
                 comprising a human immunodeficiency virus (HIV) nucleotide sequence in a 
                 See also support for claim 76. 
               
               
                 nucleic acid sample obtained from a physiological sample, which method 
                   
               
               
                 comprises the steps of: 
                   
               
               
                 (a) combining said nucleic acid sample with a single-stranded 
                   
               
               
                 nucleic acid probe comprising a sequence of at least about 32 contiguous bases 
                   
               
               
                 selected from one of the nucleotide sequences shown in FIGS. 5, 6 or 7 and 
                   
               
               
                 complementary to said HIV genomic sequence comprised in said polynucleotide, 
                   
               
               
                 said probe not forming a duplex with HTLV-I and -II nucleic acid sequences under 
                   
               
               
                 conditions of stringency for hybridization under which said probe forms a duplex 
                   
               
               
                 with said polynucleotide; and 
                   
               
               
                 (b) determining duplex formation between said probe and nucleic 
                   
               
               
                 acid present in said sample. 
                   
               
               
                 78. (new) The method of claim 61, wherein the single-stranded nucleic acid 
                 18 base BglII-SstI restriction fragment of BH10. 
               
               
                 probe is 5′- GATCTGAGCCTGGGAGCT-3′. 
                   
               
               
                 79. (new) The method of any of claims 69-73 wherein said single-stranded 
                 Discussion on p. 5 relating to the use of RNA as probe/probe template. 
               
               
                 nucleic acid comprises RNA. 
                   
               
               
                 80. (new) The method of any of claims 69-73 wherein said single-stranded 
                 p. 1, lines 28-34. discusses the use of cDNA clones of the invention to  
               
               
                 nucleic acid comprises DNA. 
                 distinguish HTLV III from HTLV I and II. 
               
               
                 81. (new) The method of claim 79 wherein said single-stranded nucleic acid 
                 Original claim 5 discusses radiolabels. 
               
               
                 further comprises a label. 
                 FIGS. 1, 3 and 4 show assays using labeled DNA. 
               
               
                 82. (new) The method of claim 80 wherein said single-stranded nucleic acid  
                 p5, lines 24-26 discuss labeled probes, and p. 7 lines 18-22 discuss nick 
               
               
                 further comprises a label. 
                 translation. 
               
               
                 83. (new) The method of claims 69-73, wherein said sample is a human 
                 p. 1 contains a discussion of the use of probes to detect HTLV-III in  
               
               
                 sample. 
                 human sera.  
               
               
                 84. (new) The method of claim 83, wherein said human sample is blood, 
                   
               
               
                 lymph or saliva. 
                   
               
               
                 85. (new) The method of claims 84, wherein said sample is blood. 
                   
               
               
                 86. (new) A method for detecting the presence of a polynucleotide 
                 page 1, lines 28-34. discusses the use of cDNA clones of the invention  
               
               
                 comprising a human immunodeficiency virus (HIV) nucleotide sequence in a 
                 to distinguish HTLV III from HTLV I and II. 
               
               
                 nucleic acid sample, the method comprising the steps of: 
                 p. 3 line 25 to p. 4 line 8 discuss regions of homology and regions of  
               
               
                 (a) combining said nucleic acid sample with a single-stranded nucleic acid  
                 variability between HTLV III, and HTLV I and II, that can be  
               
               
                 probe hybridizing under stringent conditions to an HIV nucleotide sequence present 
                 exploited in distinguishing between the different viruses. 
               
               
                 in a nucleic acid deposit selected from the group consisting of H9/HTLV-III cell 
                 p. 5, lines 12-14 discusses production of a cDNA library for  
               
               
                 line, CRL 8543; BH10, ATCC #40125; BH8, ATCC #40127; and BH5, ATCC 
                 hybridization analysis of the HIV genome. 
               
               
                 #40126, said probe not forming a duplex with HTLV-I and -II nucleic acid 
                 p. 5 line 29 - p. 6 line 10 provides support for making cDNA probes  
               
               
                 sequences under conditions of stringency for hybridization that allow said probe to  
                 from HIV mRNA. 
               
               
                 form a duplex with said polynucleotide; and 
                 p. 6, lines 23-26 discusses using cDNA probes to assay viral DNA 
               
               
                 (b) determining duplex formation between said probe and said nucleic acid 
                 p. 7, lines 18-30 discuss using an λ phage clone in southern analysis of  
               
               
                 present in said sample. 
                 restriction fragments from HIV DNA by Southern blot. 
               
               
                   
                 Statement of Deposit, p. 6. 
               
               
                   
                 Example 2, p. 8 discusses the use of stringency washes to distinguish  
               
               
                   
                 homology between HIV, HTLV I and HTLV II. 
               
               
                 87. (new) The method of claim 86, wherein the nucleic acid probe is a 
                 p. 5 lines 23-28 discuss hybridizing cDNA sequences to genomic  
               
               
                 restriction fragment. 
                 restriction fragments of HIV. 
               
               
                 88. (new) The method of claim 86, wherein the probe sequence is 
                 p. 5, lines 12-14 discusses production of a cDNA library for  
               
               
                 complementary to a sequence that is part of the gag, pol or env coding regions. 
                 hybridization analysis of the HIV genome. 
               
               
                   
                 p. 5 lines 23-28 discuss hybridizing cDNA sequences to genomic  
               
               
                   
                 restriction fragments of HIV. 
               
               
                   
                 See also, FIG. 4 (restriction map) and p. 3, line 30 to p. 4 line 3. 
               
               
                 89. (new) The method of claim 86, wherein the probe comprises DNA. 
                 Support noted for claim 1 refers to DNA probes 
               
               
                 90. (new) The method of claim 87, wherein the probe comprises DNA. 
                   
               
               
                 91. (new) A method comprising the steps of: 
                 p. 1, lines 28-34. discusses the use of cDNA clones of the invention to  
               
               
                 (a) providing a sample suspected of containing a polynucleotide; 
                 distinguish HTLV III from HTLV I and II. 
               
               
                 (b) providing a single-stranded HIV cDNA; and, 
                 p. 3 line 25 to p. 4 line 8 discuss regions of homology and regions of  
               
               
                 (c) combining said sample and said single-stranded HIV cDNA under 
                 variability between HTLV III, and HTLV I and II, that can be exploited  
               
               
                 hybridization conditions that (i) permit duplex formation between said HIV cDNA 
                 in distinguishing between the different viruses. 
               
               
                 and either nucleotide strand from a lambda bacteriophage selected from the group  
                 p. 5, lines 12-14 discusses production of a cDNA library for  
               
               
                 consisting of λBH10, λBH5 and λBH8, but (ii) do not permit duplex formation 
                 hybridization analysis of the HIV genome. 
               
               
                 with either HTLV-I or HTLV-II genomic sequences. 
                 p. 5 line 29 - p. 6 line 10 provides support for making cDNA probes  
               
               
                   
                 from HIV mRNA. 
               
               
                   
                 p. 9, lines 28-32 discusses the use of the λBH10 clone and HIV  
               
               
                   
                 genomic restriction fragments in hybridization studies. 
               
               
                   
                 p. 6, lines 23-26 discusses using cDNA probes to assay viral DNA 
               
               
                   
                 p. 7, lines 18-30 and p. 9 lines 15-26 discuss using an λ phage clone in  
               
               
                   
                 Southern analysis of restriction fragments from HIV DNA. 
               
               
                   
                 Statement of Deposit, p. 6. 
               
               
                   
                 Example 2, p. 8 discusses the use of stringency washes to distinguish  
               
               
                   
                 homology between HIV, HTLV I and HTLV II. 
               
               
                 92. (new) The method of claim 91, wherein said single-stranded nucleic 
                 FIGS. 2 and 3 provide restriction maps that would allow one of skill to  
               
               
                 acid is an SstI fragment or complement thereof. 
                 identify probes to particular genomic regions. 
               
               
                   
                 p. 9, lines 28-32 specifically discusses use probes to analyze restriction 
               
               
                   
                 fragments. Also note discussion from p. 5, line 29 to p. 6, line 26  
               
               
                   
                 regarding the use of cDNA probes 
               
               
                   
                 See also, FIG. 4 and p. 3, line 30 to p. 4 line 3. 
               
               
                 93. (new) The method of claim 91 wherein said single-stranded nucleic acid 
                 FIGS. 2 and 3 provide restriction maps that would allow one of skill to  
               
               
                 is a HindIII fragment or complement thereof. 
                 identify probes to particular genomic regions. 
               
               
                   
                 p. 9, lines 28-32 specifically discusses hybridization analysis using  
               
               
                   
                 restriction fragments. Also note discussion from p. 5, line 29 to p. 6,  
               
               
                   
                 line 26 regarding the use of cDNA sequences as probes. 
               
               
                   
                 See also, FIG. 4 and p. 3, line 30 to p. 4 line 3. 
               
               
                 94. (new) The method of claim 91 wherein said single-stranded nucleic acid  
                 FIGS. 2 and 3 provide restriction maps that would allow one of skill to  
               
               
                 comprises DNA and hybridizes to a restriction fragment generated by treating an 
                 identify probes to particular genomic regions. 
               
               
                 HIV genomic nucleic acid with HindIII and BamHI. 
                 p. 9, lines 28-32 specifically discusses use probes to analyze restriction 
               
               
                   
                 fragments. Also note discussion from p. 5, line 29 to p. 6, line 26  
               
               
                   
                 regarding the use of cDNA probes 
               
               
                   
                 See also, FIG. 4 and p. 3, line 30 to p. 4 line 3. 
               
               
                 95. (new) The method of any of claims 91-94 wherein said single-stranded 
                 p. 1, lines 28-34. discusses the use of cDNA clones of the invention to  
               
               
                 nucleic acid comprises DNA. 
                 distinguish HTLV III from HTLV I and II. 
               
               
                 96. (new) The method of claim 95 wherein said single-stranded nucleic acid  
                 Original claim 5 discusses radiolabels. 
               
               
                 further comprises a label. 
                 FIGS. 1, 3 and 4 show assays using labeled DNA. 
               
               
                   
                 p5, lines 24-26 discuss labeled probes, and p. 7 lines 18-22 discuss nick 
               
               
                   
                 translation. 
               
               
                 97. (new) The method of claim 92 wherein said single-stranded nucleic acid 
                 FIGS. 2 and 3 provide restriction maps that would allow one of skill to  
               
               
                 comprises DNA and wherein said contiguous bases are within the gag open reading 
                 identify probes to particular genomic regions. 
               
               
                 frame. 
                 p. 9, lines 28-32 specifically discusses hybridization analysis using  
               
               
                   
                 restriction fragments. Also note discussion from p. 5, line 29 to p. 6,  
               
               
                   
                 line 26 regarding the use of cDNA sequences as probes. 
               
               
                   
                 See also, FIG. 4 and p. 3, line 30 to p. 4 line 3. 
               
               
                 98. (new) The method of claim 97 wherein said single-stranded nucleic acid 
                 Original claim 5 discusses radiolabels. 
               
               
                 further comprises a label. 
                 FIGS. 1, 3 and 4 show assays using labeled DNA. 
               
               
                   
                 p5, lines 24-26 discuss labeled probes, and p. 7 lines 18-22 discuss nick 
               
               
                   
                 translation. 
               
               
                 99. (new) A method for detecting the presence of a polynucleotide 
                 page 1, lines 28-34. discusses the use of cDNA clones of the invention  
               
               
                 comprising a human immunodeficiency virus (HIV) genomic sequence in a nucleic 
                 to distinguish HTLV III from HTLV I and II. 
               
               
                 acid sample obtained from a physiological sample, which method comprises the 
                 p. 3 line 25 to p. 4 line 8 discuss regions of homology and regions of  
               
               
                 steps of: 
                 variability between HTLV III, and HTLV I and II, that can be exploited  
               
               
                 (a) combining said nucleic acid sample with a single-stranded 
                 in distinguishing between the different viruses. 
               
               
                 nucleic acid probe comprising a sequence of at least about 20 contiguous bases 
                 p. 5, lines 12-14 discusses production of a cDNA library for use in  
               
               
                 selected from the nucleotide sequences shown in FIGS. 5-7 and complementary to 
                 hybridization studies of the HIV genome. 
               
               
                 said HIV genomic sequence comprised in said polynucleotide, said probe not 
                 p. 5 line 29 - p. 6 line 10 provides support for making cDNA probes  
               
               
                 forming a duplex with HTLV-I and -II nucleic acid sequences under conditions of 
                 from HIV mRNA. 
               
               
                 stringency for hybridization under which said probe forms a duplex with said 
                 p. 6, lines 23-26 discusses using cDNA sequences for use in  
               
               
                 polynucleotide; and 
                 hybridization studies of the HIV genome. 
               
               
                 (b) determining duplex formation between said probe and nucleic 
                 p. 7, lines 18-30 discuss using an λ phage clone in Southern analysis of 
               
               
                 acid present in said sample. 
                 restriction fragments from HIV DNA. 
               
               
                   
                 Statement of Deposit, p. 6. 
               
               
                   
                 p. 1 discussion relating to detection of HIV in human sera. 
               
               
                   
                 BH10 contains an 18 base BglII-SstI restriction fragment. 
               
               
                 100. (new) The method of claim 99 wherein the probe sequence is 
                 p. 3 contains a discussion relating to the presence of gag, pol and env in  
               
               
                 complementary to a sequence which is part of the gag, pol or env open reading 
                 HTLV-III. 
               
               
                 frame. 
                   
               
               
                 101. (new) The method of claim 100 wherein the probe sequence is 
                   
               
               
                 complementary to a sequence which is part of the gag open reading frame. 
                   
               
               
                 102. (new) The method of claim 100 wherein the probe is complementary to 
                   
               
               
                 a sequence which is part of the pol open reading frame. 
                   
               
               
                 103. (new) The method of claim 99 wherein the probe comprises RNA. 
                 p. 5 discussion of the use of RNA as probe/probe template. 
               
               
                 105. (new) The method of claim 100 wherein the probe comprises RNA. 
                   
               
               
                 104. (new) The method of claim 99 wherein the probe comprises DNA. 
                 Support noted for claim 1 refers to DNA probes 
               
               
                 106. (new) The method of claim 100 wherein the probe comprises DNA. 
                   
               
               
                 107. (new) A method comprising the steps of: 
                 Support noted for claim 61 also applies here. 
               
               
                 (a) providing a sample suspected of containing a polynucleotide; 
                 p. 3 contains a discussion relating to the presence of gag, pol and env in  
               
               
                 (b) providing a single-stranded nucleic acid of 20-100 bases 
                 HTLV-III. 
               
               
                 comprising a sequence of bases of at least 20 contiguous bases selected from the  
                   
               
               
                 gag, env, or pol open reading frames; and 
                   
               
               
                 (c) combining said sample and said single-stranded nucleic acid 
                   
               
               
                 under hybridization conditions that (i) permit duplex formation between said single- 
                   
               
               
                 stranded nucleic acid and either strand of viral DNA from a lambda bacteriophage 
                   
               
               
                 selected from the group consisting of ATCC Accession no. 40143 and 40144, but 
                   
               
               
                 (ii) do not permit duplex formation with either HTLV-I or HTLV-II genomic 
                   
               
               
                 sequences. 
                   
               
               
                 108. (new) The method of any of claims 107 wherein said single-stranded 
                 p. 5 discussion of the use of RNA as probe/probe template. 
               
               
                 nucleic acid comprises RNA. 
                 Support noted for claim 1 refers to DNA probes 
               
               
                 109. (new) The method of any of claims 107 wherein said single-stranded 
                   
               
               
                 nucleic acid comprises DNA. 
                   
               
               
                 110. (new) The method of claim 108 wherein said single-stranded nucleic 
                 Original claim 5 discusses radiolabels. 
               
               
                 acid further comprises a label. 
                 FIGS. 1, 3 and 4 show assays using labeled DNA. 
               
               
                 111. (new) The method of claim 109 wherein said single-stranded nucleic 
                 p5, lines 24-26 discuss labeled probes, and p. 7 lines 18-22 discuss nick 
               
               
                 acid further comprises a label. 
                 translation. 
               
               
                 112. (new) The method of claim 108 wherein said single-stranded nucleic 
                 The paragraph spanning pages 5 and 6 discusses chemically  
               
               
                 acid is chemically synthesized at least in part. 
                 synthesizing DNA using NaOH, an RNA template, and restriction  
               
               
                 113. (new) The method of claim 109 wherein said single-stranded nucleic 
                 enzymes. 
               
               
                 acid is chemically synthesized at least in part. 
                   
               
               
                 114. (new) The method of claim 110 wherein said single-stranded nucleic 
                   
               
               
                 acid is chemically synthesized at least in part. 
                   
               
               
                 115. (new) The method of claim 111 wherein said single-stranded nucleic 
                   
               
               
                 acid is chemically synthesized at least in part. 
                   
               
               
                 116. (new) The method of claims 99 or 101 wherein said sample is a human 
                 p. 1 contains a discussion of the use of probes to detect HTLV-III in  
               
               
                 sample. 
                 human sera. 
               
               
                 117. (new) The method of claim 116 wherein said human sample is blood, 
                   
               
               
                 lymph or saliva. 
                   
               
               
                 118. (new) The method of claims 99 or 101 wherein said sample is blood, 
                   
               
               
                 lymph or saliva. 
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
             
           
               
                 TABLE II 
               
               
                   
               
               
                 (BH 5 and 8 v. LUCIW) 
               
               
                 89.8% identity  
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                                 10        20        30        40        50 
               
               
                 BH 8   -TGGAAGGGCTAATTCACTCCCAACGAAGACAAGATATCCTTGATCTGTGGATCCACCAC 
               
               
                         ::::::::::::::   :::::: :::::::::: :::::::::::::::::: ::::: 
               
               
                 Licuw, CTGGAAGGGCTAATTTGGTCCCAAAGAAGACAAGAGATCCTTGATCTGTGGATCTACCAC 
               
               
                                10        20        30        40        50        60 
               
               
                   
               
               
                       60        70        80        90       100       110 
               
               
                 BH 8   ACACAAGGCTACTTCCCTGATTGGCAGAACTACACACCAGGGCCAGGAGTCAGATATCCA 
               
               
                        ::::::::::::::::::::::::::::: :::::::::::::::::  ::::::::::: 
               
               
                 Licuw, ACACAAGGCTACTTCCCTGATTGGCAGAATTACACACCAGGGCCAGGGATCAGATATCCA 
               
               
                                70        80        90       100       110       120 
               
               
                   
               
               
                      120       130       140       150       160       170 
               
               
                 BH 8   CTGACCTTTGGATGGTGCTACAAGCTAGTACCAGTTGAGCCAGAGAAGTAAGAAGAAGCC 
               
               
                        ::::::::::::::::::: ::::::::::::::::::::::::::::  :::::: ::: 
               
               
                 Licuw, CTGACCTTTGGATGGTGCTTCAAGCTAGTACCAGTTGAGCCAGAGAAGGTAGAAGAGGCC 
               
               
                               130       140       150       160       170       180 
               
               
                   
               
               
                      180       190       200       210       220       230 
               
               
                 BH 8   AATAAAGGAGAGAACACCAGCTTGTTACACCCTGTGAGCCTGCATGGAATTGATGACCCG 
               
               
                        ::: :::::::::::: :::::::::::::::: ::::::::::::: :: :: ::: :: 
               
               
                 Licuw, AATGAAGGAGAGAACAACAGCTTGTTACACCCTATGAGCCTGCATGGGATGGAGGACGCG 
               
               
                               190       200       210       220       230       240 
               
               
                   
               
               
                      240       250       260       270       280       290 
               
               
                 BH 8   GAGAGAGAAGTGTTAGAGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACATGGCCCGA 
               
               
                        :::: ::::::::::: ::::::::::::::::   :::::::::::::::::::::::: 
               
               
                 Licuw, GAGAAAGAAGTGTTAGTGTGGAGGTTTGACAGCAAACTAGCATTTCATCACATGGCCCGA 
               
               
                               250       260       270       280       290       300 
               
               
                   
               
               
                      300       310       320       330       340       350 
               
               
                 BH 8   GAGCTGCATCCGGAGTACTTCAAGAACTGCTGACATCGAGCTTGCTACAAGGGACTTTCC 
               
               
                        ::::::::::::::::::: :::  :::::::::::::::::: :::::::::::::::: 
               
               
                 Licuw, GAGCTGCATCCGGAGTACTACAAAGACTGCTGACATCGAGCTTTCTACAAGGGACTTTCC 
               
               
                               310       320       330       340       350       360 
               
               
                   
               
               
                      360       370       380       390       400       410 
               
               
                 BH 8   GCTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGAGCCCTCAGA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::  :::::::: 
               
               
                 Licuw, GCTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGT-CCCTCAGA 
               
               
                               370       380       390       400       410 
               
               
                   
               
               
                      420       430        440       450       460       470 
               
               
                 BH 8   TCCTGCATATAAGCAG-CTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCT 
               
               
                        : :::::::::::::: ::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TGCTGCATATAAGCAGACTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCT 
               
               
                      420       430       440       450       460       470 
               
               
                   
               
               
                       480       490 
               
               
                 BH 8   GAGCCTGGGAGCTC---------------------------------------------- 
               
               
                        :::::::::::::: 
               
               
                 Licuw, GAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGC 
               
               
                      480       490       500       510       520       530 
               
               
                   
               
               
                 ------------------------------------------------------------------- 
               
               
                   
               
               
                 Licuw, CTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCC 
               
               
                      540       550       560       570       580       590 
               
               
                   
               
               
                 ------------------------------------------------------------------- 
               
               
                   
               
               
                 Licuw, TCAGACCCTTTTAGTCAGTGTGGAAAAATCTCTAGCAGTGGCGCCCGAACAGGGACGCGA 
               
               
                      600       610       620       630       640       650 
               
               
                   
               
               
                                                 500       510       520       530 
               
               
                 BH 5   --------------------GAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCA 
               
               
                                            :::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AAGCGAAAGTAGAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCA 
               
               
                      660       670       680       690       700       710 
               
               
                   
               
               
                             540       550       560       570       580       590 
               
               
                 BH 5   CGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCT 
               
               
                        : :::::::::::::::::::::::::::::::::::::   :::::::::::::::::: 
               
               
                 Licuw, CAGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAT--TTTTGACTAGCGGAGGCT 
               
               
                      720       730       740       750         760       770 
               
               
                   
               
               
                             600         610       620       630       640       650 
               
               
                 BH 5   AGAAGGAGAGAGA--TGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGAT 
               
               
                        :::::::::::::  :::::::::::::::: ::::::::::::::::::::::::  :: 
               
               
                 Licuw, AGAAGGAGAGAGAGATGGGTGCGAGAGCGTCGGTATTAAGCGGGGGAGAATTAGATAAAT 
               
               
                        780       790       800       810       820       830 
               
               
                   
               
               
                               660       670       680       690       700       710 
               
               
                 BH 5   GGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTAT 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::: 
               
               
                 Licuw, GGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAGTTAAAACATATAGTAT 
               
               
                        840       850       860       870       880       890 
               
               
                   
               
               
                               720       730       740       750       760       770 
               
               
                 BH 5   GGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAG 
               
               
                        ::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::: 
               
               
                 Licuw, GGGCAAGCAGGGAGCTAGAACGATTCGCAGTCAATCCTGGCCTGTTAGAAACATCAGAAG 
               
               
                        900       910       920       930       940       950 
               
               
                   
               
               
                               780       790       800       810       820       830 
               
               
                 BH 5   GCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTA 
               
               
                        :::: ::::::::: ::::::::::::: ::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GCTGCAGACAAATATTGGGACAGCTACAGCCATCCCTTCAGACAGGATCAGAAGAACTTA 
               
               
                        960       970       980       990      1000      1010 
               
               
                   
               
               
                               840       850       860       870       880       890 
               
               
                 BH 5   GATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAG 
               
               
                        ::::::::::::::::::::::::::::::::::::: ::::::::::::::  :::::: 
               
               
                 Licuw, GATCATTATATAATACAGTAGCAACCCTCTATTGTGTACATCAAAGGATAGATGTAAAAG 
               
               
                       1020      1030      1040      1050      1060      1070 
               
               
                   
               
               
                               900       910       920       930       940       950 
               
               
                 BH 5   ACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGAAAAAAGCAC 
               
               
                        ::::::::::::::::::: ::::::::::::::::::::::::::::::::::: :::: 
               
               
                 Licuw, ACACCAAGGAAGCTTTAGAGAAGATAGAGGAAGAGCAAAACAAAAGTAAGAAAAAGGCAC 
               
               
                       1080      1090      1100      1110      1120      1130 
               
               
                   
               
               
                               960             970       980       990      1000 
               
               
                 BH 5   AGCAAGCAGCAGCTGA------CACAGGACACAGCAGTCAGGTCAGCCAAAATTACCCTA 
               
               
                        :::::::::::::::       ::::::::::::::: :::::::::::::::::::::: 
               
               
                 Licuw, AGCAAGCAGCAGCTGCAGCTGGCACAGGAAACAGCAGCCAGGTCAGCCAAAATTACCCTA 
               
               
                       1140      1150      1160      1170      1180      1190 
               
               
                   
               
               
                          1010      1020      1030      1040      1050      1060 
               
               
                 BH 5   TAGTGCAGAACATCCAGGGGCAAATGGTACATCAGGCCATATCACCTAGAACTTTAAATG 
               
               
                        ::::::::::: : :::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAGTGCAGAACCTACAGGGGCAAATGGTACATCAGGCCATATCACCTAGAACTTTAAATG 
               
               
                       1200      1210      1220      1230      1240      1250  
               
               
                   
               
               
                          1070      1080      1090      1100      1110      1120 
               
               
                 BH 5   CATGGGTAAAAGTAGTAGAAGAGAAGGCTTTCAGCCCAGAAGTAATACCCATGTTTTCAG 
               
               
                        :::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CATGGGTAAAAGTAGTAGAAGAAAAGGCTTTCAGCCCAGAAGTAATACCCATGTTTTCAG 
               
               
                       1260      1270      1280      1290      1300      1310 
               
               
                   
               
               
                          1130      1140      1150      1160      1170      1180 
               
               
                 BH 5   CATTATCAGAAGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGAC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CATTATCAGAAGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGAC 
               
               
                       1320      1330      1340      1350      1360      1370 
               
               
                   
               
               
                          1190      1200      1210      1220      1230      1240 
               
               
                 BH 5   ATCAAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGAATGGGATA 
               
               
                        ::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::: 
               
               
                 Licuw, ATCAAGCAGCCATGCAAATGTTAAAAGAGACTATCAATGAGGAAGCTGCAGAATGGGATA 
               
               
                       1380      1390      1400      1410      1420      1430 
               
               
                   
               
               
                          1250      1260      1270      1280      1290      1300 
               
               
                 BH 5   GAGTGCATCCAGTGCATGCAGGGCCTATCGCACCAGGCCAGATGAGAGAACCAAGGGGAA 
               
               
                        :::::::::::::::::::::::::::: ::::::::::: ::::::::::::::::::: 
               
               
                 Licuw, GAGTGCATCCAGTGCATGCAGGGCCTATTGCACCAGGCCAAATGAGAGAACCAAGGGGAA 
               
               
                       1440      1450      1460      1470      1480      1490 
               
               
                   
               
               
                          1310      1320      1330      1340      1350      1360 
               
               
                 BH 5   GTGACATAGCAGGAACTACTAGTACCCTTCAGGAACAAATAGGATGGATGACAAATAATC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GTGACATAGCAGGAACTACTAGTACCCTTCAGGAACAAATAGGATGGATGACAAATAATC 
               
               
                       1500      1510      1520      1530      1540      1550 
               
               
                   
               
               
                          1370      1380      1390      1400      1410      1420 
               
               
                 BH 5   CACCTATCCCAGTAGGAGAAATTTATAAAAGATGGATAATCCTGGGATTAAATAAAATAG 
               
               
                        :::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CACCTATCCCAGTAGGAGAAATCTATAAAAGATGGATAATCCTGGGATTAAATAAAATAG 
               
               
                       1560      1570      1580      1590      1600      1610 
               
               
                   
               
               
                          1430      1440      1450      1460      1470      1480 
               
               
                 BH 5   TAAGGATGTATAGTCCTACCAGCATTCTGGACATAAGACAAGGACCAAAGGAACCCTTTA 
               
               
                        :::: :::::::: :::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAAGAATGTATAGCCCTACCAGCATTCTGGACATAAGACAAGGACCAAAGGAACCCTTTA 
               
               
                       1620      1630      1640      1650      1660      1670 
               
               
                   
               
               
                          1490      1500      1510      1520      1530      1540 
               
               
                 BH 5   GAGACTATGTAGACCGGTTCTATAAAACTCTAAGAGCCGAGCAAGCTTCACAGGAAGTAA 
               
               
                        :::: ::::::::::::::::::::::::::::::::::: :::::::::::::: :::: 
               
               
                 Licuw, GAGATTATGTAGACCGGTTCTATAAAACTCTAAGAGCCGAACAAGCTTCACAGGATGTAA 
               
               
                       1680      1690      1700      1710      1720      1730 
               
               
                   
               
               
                          1550      1560      1570      1580      1590      1600 
               
               
                 BH 5   AAAATTGGATGACAGAAACCTTGTTGGTCCAAAATGCGAACCCAGATTGTAAGACTATTT 
               
               
                        ::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::: 
               
               
                 Licuw, AAAATTGGATGACAGAAACCTTGTTGGTCCAAAATGCAAACCCAGATTGTAAGACTATTT 
               
               
                       1740      1750      1760      1770      1780      1790 
               
               
                   
               
               
                          1610      1620      1630      1640      1650      1660 
               
               
                 BH 5   TAAAAGCATTGGGACCAGCAGCTACTCTAGAAGAAATGATGACAGCATGTCAGGGAGTGG 
               
               
                        ::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAAAAGCATTGGGACCAGCAGCTACACTAGAAGAAATGATGACAGCATGTCAGGGAGTGG 
               
               
                       1800      1810      1820      1830      1840      1850 
               
               
                   
               
               
                          1670      1680      1690      1700      1710      1720 
               
               
                 BH 5   GAGGACCCGGCCATAAAGCAAGAGTTTTGGCTGAAGCAATGAGCCAAGTAACAAATTCAA 
               
               
                        : ::::::::::::::::::::::::::::::::::: :::::::::::::::::: :: 
               
               
                 Licuw, GGGGACCCGGCCATAAAGCAAGAGTTTTGGCTGAAGCCATGAGCCAAGTAACAAATCCAG 
               
               
                       1860      1870      1880      1890      1900      1910 
               
               
                   
               
               
                          1730      1740      1750      1760      1770      1780 
               
               
                 BH 5   CTACCATAATGATGCAAAGAGGCAATTTTAGGAACCAAAGAAAGATTGTTAAGTGTTTCA 
               
               
                        ::: :::::::::::: :::::::::::::::::::::::::::: :::::::::::::: 
               
               
                 Licuw, CTAACATAATGATGCAGAGAGGCAATTTTAGGAACCAAAGAAAGACTGTTAAGTGTTTCA 
               
               
                       1920      1930      1940      1950      1960      1970 
               
               
                   
               
               
                          1790      1800      1810      1820      1830      1840 
               
               
                 BH 5   ATTGTGGCAAAGAAGGGCACATAGCAAGAAATTGCAAGGCCCCTAGAAAAAGAGGCTGTT 
               
               
                        :::::::::::::::: :::::::: : :::::::: ::::::::: :::: :::  ::: 
               
               
                 Licuw, ATTGTGGCAAAGAAGG-CACATAGCCAAAAATTGCAGGGCCCCTAGGAAAA-AGG--GTT 
               
               
                       1980      1990       2000      2010      2020       2030 
               
               
                   
               
               
                          1850      1860      1870      1880      1890      1900 
               
               
                 BH 5   GGAAATGTGGAAAGGAAGGACACCAAATGAAAGATTGTACTGAGAGACAGGCTAATTTTT 
               
               
                         : : ::::::: :::::::::::::::::::::::: :::::::::::::::::::::: 
               
               
                 Licuw, TGGAGTGTGGAAGGGAAGGACACCAAATGAAAGATTGCACTGAGAGACAGGCTAATTTTT 
               
               
                           2040      2050      2060      2070      2080      2090 
               
               
                   
               
               
                          1910      1920      1930      1940      1950      1960 
               
               
                 BH 5   TAGGGAAGATCTGGCCTTCCTACAAGGGAAGGCCAGGGAATTTTCTTCAGAGCAGACCAG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAGGGAAGATCTGGCCTTCCTACAAGGGAAGGCCAGGGAATTTTCTTCAGAGCAGACCAG 
               
               
                           2100      2110      2120      2130      2140      2150 
               
               
                   
               
               
                          1970      1980      1990      2000      2010      2020 
               
               
                 BH 5   AGCCAACAGCCCCACCATTTCTTCAGAGCAGACCAGAGCCAACAGCCCCACCAGAAGAGA 
               
               
                        :::::::::::::::::                                    ::::::: 
               
               
                 Licuw, AGCCAACAGCCCCACCA------------------------------------GAAGAGA 
               
               
                           2160      2170 
               
               
                   
               
               
                          2030      2040      2050      2060      2070      2080 
               
               
                 BH 5   GCTTCAGGTCTGGGGTAGAGACAACAACTCCCTCTCAGAAGCAGGAGCCGATAGACAAGG 
               
               
                        ::::::::: :::::  :::: :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GCTTCAGGTTTGGGGAGGAGAAAACAACTCCCTCTCAGAAGCAGGAGCCGATAGACAAGG 
               
               
                       2180      2190      2200      2210      2220      2230 
               
               
                   
               
               
                          2090      2100      2110      2120      2130      2140 
               
               
                 BH 5   AACTGTATCCTTTAACTTCCCTCAGATCACTCTTTGGCAACGACCCCTCGTCACAATAAA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: 
               
               
                 Licuw, AACTGTATCCTTTAACTTCCCTCAGATCACTCTTTGGCAACGACCCCTCGTCACAATTAA 
               
               
                       2240      2250      2260      2270      2280      2290 
               
               
                   
               
               
                           2150      2160      2170      2180      2190      2200 
               
               
                 BH 5   G-ATAGGGGGGCAACTAAAGGAAGCTCTATTAGATACAGGAGCAGATGATACAGTATTAG 
               
               
                        : :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GGATAGGGGGGCAACTAAAGGAAGCTCTATTAGATACAGGAGCAGATGATACAGTATTAG 
               
               
                       2300      2310      2320      2330      2340      2350 
               
               
                   
               
               
                           2210      2220      2230      2240      2250      2260 
               
               
                 BH 5   AAGAAATGAGTTTGCCAGGAAGATGGAAACCAAAAATGATAGGGGGAATTGGAGGTTTTA 
               
               
                        ::::::::: ::::::::::: :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AAGAAATGAATTTGCCAGGAAAATGGAAACCAAAAATGATAGGGGGAATTGGAGGTTTTA 
               
               
                       2360      2370      2380      2390      2400      2410 
               
               
                   
               
               
                           2270      2280      2290      2300      2310      2320 
               
               
                 BH 5   TCAAAGTAAGACAGTATGATCAGATACTCATAGAAATCTGTGGACATAAAGCTATAGGTA 
               
               
                        :::::::::::::::: ::::::::::   :::::::::::::::::::::::::::::: 
               
               
                 Licuw, TCAAAGTAAGACAGTACGATCAGATACCTGTAGAAATCTGTGGACATAAAGCTATAGGTA 
               
               
                       2420      2430      2440      2450      2460      2470 
               
               
                   
               
               
                           2330      2340      2350      2360      2370      2380 
               
               
                 BH 5   CAGTATTAGTAGGACCTACACCTGTCAACATAATTGGAAGAAATCTGTTGACTCAGATTG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CAGTATTAGTAGGACCTACACCTGTCAACATAATTGGAAGAAATCTGTTGACTCAGATTG 
               
               
                       2480      2490      2500      2510      2520      2530 
               
               
                   
               
               
                           2390      2400      2410      2420      2430      2440 
               
               
                 BH 5   GTTGCACTTTAAATTTTCCCATTAGTCCTATTGAAACTGTACCAGTAAAATTAAAGCCAG 
               
               
                        :::: ::::::::::: ::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GTTGTACTTTAAATTTCCCCATTAGTCCTATTGAAACTGTACCAGTAAAATTAAAGCCAG 
               
               
                       2540      2550      2560      2570      2580      2590 
               
               
                   
               
               
                           2450      2460      2470      2480      2490      2500 
               
               
                 BH 5   GAATGGATGGCCCAAAAGTTAAACAATGGCCATTGACAGAAGAAAAAATAAAAGCATTAG 
               
               
                        :::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GAATGGATGGCCCAAAAGTTAAGCAATGGCCATTGACAGAAGAAAAAATAAAAGCATTAG 
               
               
                       2600      2610      2620      2630      2640      2650 
               
               
                   
               
               
                           2510      2520      2530      2540      2550      2560 
               
               
                 BH 5   TAGAAATTTGTACAGAAATGGAAAAGGAAGGGAAAATTTCAAAAATTGGGCCTGAAAATC 
               
               
                        :::: :: :::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAGAGATATGTACAGAAATGGAAAAGGAAGGGAAAATTTCAAAAATTGGGCCTGAAAATC 
               
               
                       2660      2670      2680      2690      2700      2710 
               
               
                   
               
               
                           2570      2580      2590      2600      2610      2620 
               
               
                 BH 5   CATACAATACTCCAGTATTTGCCATAAAGAAAAAAGACAGTACTAAATGGAGAAAATTAG 
               
               
                        :::::::::::::::::::::: ::::::::::::::::::::::::::::::::: ::: 
               
               
                 Licuw, CATACAATACTCCAGTATTTGCTATAAAGAAAAAAGACAGTACTAAATGGAGAAAACTAG 
               
               
                       2720      2730      2740      2750      2760      2770 
               
               
                   
               
               
                           2630      2640      2650      2660      2670      2680 
               
               
                 BH 5   TAGATTTCAGAGAACTTAATAGGAGAACTCAAGACTTCTGGGAAGTTCAATTGGGAATAC 
               
               
                        :::::::::::::::::::::  :::::::::::::::::::::::::: :: ::::::: 
               
               
                 Licuw, TAGATTTCAGAGAACTTAATAAAAGAACTCAAGACTTCTGGGAAGTTCAGTTAGGAATAC 
               
               
                       2780      2790      2800      2810      2820      2830 
               
               
                   
               
               
                           2690      2700       2710      2720      2730      2740 
               
               
                 BH 5   CACATCCCGCAGGGTTAAAAAA-GAAAAAATCAGTAACAGTACTGGATGTGGGTGATGCA 
               
               
                        :::: ::::::::::::::::: ::::::::::::::::::: ::::::::::::::::: 
               
               
                 Licuw, CACA-CCCGCAGGGTTAAAAAAAGAAAAAATCAGTAACAGTATTGGATGTGGGTGATGCA 
               
               
                       2840       2850      2860      2870      2880      2890 
               
               
                   
               
               
                            2750      2760      2770      2780      2790      2800 
               
               
                 BH 5   TATTTTTCAGTTCCCTTAGATGAAGACTTCAGGAAGTATACTGCATTTACCATACCTAGT 
               
               
                        :: :::::::::::::::::: ::::::: :: ::::::::::::::::::::::::::: 
               
               
                 Licuw, TACTTTTCAGTTCCCTTAGATAAAGACTTTAGAAAGTATACTGCATTTACCATACCTAGT 
               
               
                        2900      2910      2920      2930      2940      2950 
               
               
                   
               
               
                            2810      2820      2830      2840       2850      2860 
               
               
                 BH 5   ATAAATAATGAGACACCAGGGATTAGATATCAGTACAATGTG-CTTCCACAGGGATGGAA 
               
               
                        ::::: :::::::::::::::::::::::::::::::::::: :: :::::::::::::: 
               
               
                 Licuw, ATAAACAATGAGACACCAGGGATTAGATATCAGTACAATGTGGCTGCCACAGGGATGGAA 
               
               
                        2960      2970      2980      2990      3000      3010 
               
               
                   
               
               
                             2870      2880      2890      2900      2910      2920 
               
               
                 BH 5   AGGATCACCAGCAATATTCCAAAGTAGCATGACAAAAATCTTAGAGCCTTTTAGAAAACA 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AGGATCACCAGCAATATTCCAAAGTAGCATGACAAAAATCTTAGAGCCTTTTAGAAAACA 
               
               
                        3020      3030      3040      3050      3060      3070 
               
               
                   
               
               
                             2930      2940      2950      2960      2970      2980 
               
               
                 BH 5   AAATCCAGACATAGTTATCTATCAATACATGGATGATTTGTATGTAGGATCTGACTTAGA 
               
               
                         ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GAATCCAGACATAGTTATCTATCAATACATGGATGATTTGTATGTAGGATCTGACTTAGA 
               
               
                        3080      3090      3100      3110      3120      3130 
               
               
                   
               
               
                             2990      3000      3010      3020      3030      3040 
               
               
                 BH 5   AATAGGGCAGCATAGAACAAAAATAGAGGAGCTGAGACAACATCTGTTGAGGTGGGGATT 
               
               
                        :::::::::::::::::::::::::::::: :::::::: :::::::::::::::::::: 
               
               
                 Licuw, AATAGGGCAGCATAGAACAAAAATAGAGGAACTGAGACAGCATCTGTTGAGGTGGGGATT 
               
               
                        3140      3150      3160      3170      3180      3190 
               
               
                   
               
               
                             3050      3060      3070      3080      3090      3100 
               
               
                 BH 5   TACCACACCAGACAAAAAACATCAGAAAGAACCTCCATTCCTTTGGATGGGTTATGAACT 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TACCACACCAGACAAAAAACATCAGAAAGAACCTCCATTCCTTTGGATGGGTTATGAACT 
               
               
                        3200      3210      3220      3230      3240      3250 
               
               
                   
               
               
                             3110      3120      3130      3140      3150      3160 
               
               
                 BH 5   CCATCCTGATAAATGGACAGTACAGCCTATAGTGCTGCCAGAAAAAGACAGCTGGACTGT 
               
               
                        ::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::: 
               
               
                 Licuw, CCATCCTGATAAATGGACAGTACAGCCTATAATGCTGCCAGAAAAAGACAGCTGGACTGT 
               
               
                        3260      3270      3280      3290      3300      3310 
               
               
                   
               
               
                             3170      3180      3190      3200      3210      3220 
               
               
                 BH 5   CAATGACATACAGAAGTTAGTGGGAAAATTGAATTGGGCAAGTCAGATTTATCCAGGGAT 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::: 
               
               
                 Licuw, CAATGACATACAGAAGTTAGTGGGAAAATTGAATTGGGCAAGTCAGATTTATGCAGGGAT 
               
               
                        3320      3330      3340      3350      3360      3370 
               
               
                   
               
               
                             3230      3240      3250      3260      3270      3280 
               
               
                 BH 5   TAAAGTAAGGCAATTATGTAAACTCCTTAGAGGAACCAAAGCACTAACAGAAGTAATACC 
               
               
                        :::::::: ::: ::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAAAGTAAAGCAGTTATGTAAACTCCTTAGAGGAACCAAAGCACTAACAGAAGTAATACC 
               
               
                        3380      3390      3400      3410      3420      3430 
               
               
                   
               
               
                             3290      3300      3310      3320      3330      3340 
               
               
                 BH 5   ACTAACAGAAGAAGCAGAGCTAGAACTGGCAGAAAACAGAGAGATTCTAAAAGAACCAGT 
               
               
                        ::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::: 
               
               
                 Licuw, ACTAACAGAAGAAGCAGAGCTAGAACTGGCAGAAAACAGGGAGATTCTAAAAGAACCAGT 
               
               
                        3440      3450      3460      3470      3480      3490 
               
               
                   
               
               
                             3350      3360      3370      3380      3390      3400 
               
               
                 BH 5   ACATGGAGTGTATTATGACCCATCAAAAGACTTAATAGCAGAAATACAGAAGCAGGGGCA 
               
               
                        ::::: ::: :::::::::::::::::::::::: ::::::::::::::::::::::::: 
               
               
                 Licuw, ACATGAAGTATATTATGACCCATCAAAAGACTTAGTAGCAGAAATACAGAAGCAGGGGCA 
               
               
                        3500      3510      3520      3530      3540      3550 
               
               
                   
               
               
                             3410      3420      3430      3440      3450      3460 
               
               
                 BH 5   AGGCCAATGGACATATCAAATTTATCAAGAGCCATTTAAAAATCTGAAAACAGGAAAATA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: 
               
               
                 Licuw, AGGCCAATGGACATATCAAATTTATCAAGAGCCATTTAAAAATCTGAAAACAGGAAAGTA 
               
               
                        3560      3570      3580      3590      3600      3610 
               
               
                   
               
               
                             3470      3480      3490      3500      3510      3520 
               
               
                 BH 5   TGCAAGAATGAGGGGTGCCCACACTAATGATGTAAAACAATTAACAGAGGCAGTGCAAAA 
               
               
                        :::::: :::::::::::::::::::::::::::::::: :::::::::::::::::::: 
               
               
                 Licuw, TGCAAGGATGAGGGGTGCCCACACTAATGATGTAAAACAGTTAACAGAGGCAGTGCAAAA 
               
               
                        3620      3630      3640      3650      3660      3670 
               
               
                   
               
               
                             3530      3540      3550      3560      3570      3580 
               
               
                 BH 5   AATAACCACAGAAAGCATAGTAATATGGGGAAAGACTCCTAAATTTAAACTACCCATACA 
               
               
                        : :: :::::::::::::::::::::::::::::: :::::::::::::::::::::::: 
               
               
                 Licuw, AGTATCCACAGAAAGCATAGTAATATGGGGAAAGATTCCTAAATTTAAACTACCCATACA 
               
               
                        3680      3690      3700      3710      3720      3730 
               
               
                   
               
               
                             3590      3600      3610      3620      3630      3640 
               
               
                 BH 5   AAAAGAAACATGGGAAACATGGTGGACAGAGTATTGGCAAGCCACCTGGATTCCTGAGTG 
               
               
                        ::: :::::::::::: :::::::::  :::::::::::::: ::::::::::::::::: 
               
               
                 Licuw, AAAGGAAACATGGGAAGCATGGTGGATGGAGTATTGGCAAGCTACCTGGATTCCTGAGTG 
               
               
                        3740      3750      3760      3770      3780      3790 
               
               
                   
               
               
                             3650      3660      3670      3680      3690      3700 
               
               
                 BH 5   GGAGTTTGTTAATACCCCTCCTTTAGTGAAATTATGGTACCAGTTAGAGAAAGAACCCAT 
               
               
                        ::::::::: ::::::::::: :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GGAGTTTGTCAATACCCCTCCCTTAGTGAAATTATGGTACCAGTTAGAGAAAGAACCCAT 
               
               
                        3800      3810      3820      3830      3840      3850 
               
               
                   
               
               
                             3710      3720      3730      3740      3750      3760 
               
               
                 BH 5   AGTAGGAGCAGAAACCTTCTATGTAGATGGGGCAGCTAGCAGGGAGACTAAATTAGGAAA 
               
               
                        ::::::::::::::: ::::::::::::::::::::::  :::::::::::::::::::: 
               
               
                 Licuw, AGTAGGAGCAGAAACTTTCTATGTAGATGGGGCAGCTAATAGGGAGACTAAATTAGGAAA 
               
               
                        3860      3870      3880      3890      3900      3910 
               
               
                   
               
               
                             3770      3780      3790      3800      3810      3820 
               
               
                 BH 5   AGCAGGATATGTTACTAATAGAGGAAGACAAAAAGTTGTCACCCTAACTGACACAACAAA 
               
               
                        :::::::::::::::: : ::::::::::::::::::::: :: :: ::::::::::::: 
               
               
                 Licuw, AGCAGGATATGTTACTGACAGAGGAAGACAAAAAGTTGTCTCCATAGCTGACACAACAAA 
               
               
                        3920      3930      3940      3950      3960      3970 
               
               
                   
               
               
                             3830      3840      3850      3860      3870      3880 
               
               
                 BH 5   TCAGAAGACTGAATTACAAGCAATTCATCTAGCTTTGCAGGATTCGGGATTAGAAGTAAA 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TCAGAAGACTGAATTACAAGCAATTCATCTAGCTTTGCAGGATTCGGGATTAGAAGTAAA 
               
               
                        3980      3990      4000      4010      4020      4030 
               
               
                   
               
               
                             3890      3900      3910      3920      3930      3940 
               
               
                 BH 5   TATAGTAACAGACTCACAATATGCATTAGGAATCATTCAAGCACAACCAGATAAAAGTGA 
               
               
                         ::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::: 
               
               
                 Licuw, CATAGTAACAGACTCACAATATGCATTAGGAATCATTCAAGCACAACCAGATAAGAGTGA 
               
               
                        4040      4050      4060      4070      4080      4090 
               
               
                   
               
               
                             3950      3960      3970      3980      3990      4000 
               
               
                 BH 5   ATCAGAGTTAGTCAATCAAATAATAGAGCAGTTAATAAAAAAGGAAAAGGTCTATCTGGC 
               
               
                        :::::::::::::: ::::::::::::::::::::::::::::::::::::::: ::::: 
               
               
                 Licuw, ATCAGAGTTAGTCAGTCAAATAATAGAGCAGTTAATAAAAAAGGAAAAGGTCTACCTGGC 
               
               
                        4100      4110      4120      4130      4140      4150 
               
               
                   
               
               
                             4010      4020      4030      4040      4050      4060 
               
               
                 BH 5   ATGGGTACCAGCACACAAAGGAATTGGAGGAAATGAACAAGTAGATAAATTAGTCAGTGC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ATGGGTACCAGCACACAAAGGAATTGGAGGAAATGAACAAGTAGATAAATTAGTCAGTGC 
               
               
                        4160      4170      4180      4190      4200      4210 
               
               
                   
               
               
                             4070      4080      4090      4100      4110      4120 
               
               
                 BH 5   TGGAATCAGGAAAATACTATTTTTAGATGGAATAGATAAGGCCCAAGAAGAACATGAGAA 
               
               
                        ::::::::::::: ::::::::::  :::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TGGAATCAGGAAAGTACTATTTTTGAATGGAATAGATAAGGCCCAAGAAGAACATGAGAA 
               
               
                        4220      4230      4240      4250      4260      4270 
               
               
                   
               
               
                             4130      4140      4150      4160      4170      4180 
               
               
                 BH 5   ATATCACAATAATTGGAGAGCAATGGCTAGTGATTTTAACCTGCCACCTGTAGTAGCAAA 
               
               
                        :::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ATATCACAGTAATTGGAGAGCAATGGCTAGTGATTTTAACCTGCCACCTGTAGTAGCAAA 
               
               
                        4280      4290      4300      4310      4320      4330 
               
               
                   
               
               
                             4190      4200      4210      4220      4230      4240 
               
               
                 BH 5   AGAAATAGTAGCCAGCTGTGATAAATGTCAGCTAAAAGGAGAAGCCATGCATGGACAAGT 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AGAAATAGTAGCCAGCTGTGATAAATGTCAGCTAAAAGGAGAAGCCATGCATGGACAAGT 
               
               
                        4340      4350      4360      4370      4380      4390 
               
               
                   
               
               
                             4250      4260      4270      4280      4290      4300 
               
               
                 BH 5/8 AGACTGTAGTCCAGGAATATGGCAACTAGATTGTACACATTTAGAAGGAAAAGTTATCCT 
               
               
                        :::::::::::::::::::::::::::::::::::::::: ::::::::::: ::::::: 
               
               
                 Licuw, AGACTGTAGTCCAGGAATATGGCAACTAGATTGTACACATCTAGAAGGAAAAATTATCCT 
               
               
                        4400      4410      4420      4430      4440      4450 
               
               
                   
               
               
                             4310      4320      4330      4340      4350      4360 
               
               
                 BH 5   GGTAGCAGTTCATGTAGCCAGTGGATATATAGAAGCAGAAGTTATTCCAGCAGAAACAGG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::: 
               
               
                 Licuw, GGTAGCAGTTCATGTAGCCAGTGGATATATAGAAGCAGAAGTTATTCCAGCAGAGACAGG 
               
               
                        4460      4470      4480      4490      4500      4510 
               
               
                   
               
               
                             4370      4380      4390      4400      4410      4420 
               
               
                 BH 5   GCAGGAAACAGCATATTTTCTTTTAAAATTAGCAGGAAGATGGCCAGTAAAAACAATACA 
               
               
                        ::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GCAGGAAACAGCATATTTTCTCTTAAAATTAGCAGGAAGATGGCCAGTAAAAACAATACA 
               
               
                        4520      4530      4540      4550      4560      4570 
               
               
                   
               
               
                             4430      4440      4450      4460      4470      4480 
               
               
                 BH 5   TACAGACAATGGCAGCAATTTCACCAGTGCTACGGTTAAGGCCGCCTGTTGGTGGGCGGG 
               
               
                        :::::::::::::::::::::::::::: :::::::::::::::::::::::::::: :: 
               
               
                 Licuw, TACAGACAATGGCAGCAATTTCACCAGTACTACGGTTAAGGCCGCCTGTTGGTGGGCAGG 
               
               
                        4580      4590      4600      4610      4620      4630 
               
               
                   
               
               
                             4490      4500      4510      4520      4530      4540 
               
               
                 BH 5   AATCAAGCAGGAATTTGGAATTCCCTACAATCCCCAAAGTCAAGGAGTAGTAGAATCTAT 
               
               
                         ::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GATCAAGCAGGAATTTGGCATTCCCTACAATCCCCAAAGTCAAGGAGTAGTAGAATCTAT 
               
               
                        4640      4650      4660      4670      4680      4690 
               
               
                   
               
               
                             4550      4560      4570      4580      4590      4600 
               
               
                 BH 5   GAATAAAGAATTAAAGAAAATTATAGGACAGGTAAGAGATCAGGCTGAACATCTTAAGAC 
               
               
                        :::::: :::::::::::::::::::::::::::::::::::::::::::: :::::::: 
               
               
                 Licuw, GAATAATGAATTAAAGAAAATTATAGGACAGGTAAGAGATCAGGCTGAACACCTTAAGAC 
               
               
                        4700      4710      4720      4730      4740      4750 
               
               
                   
               
               
                             4610      4620      4630      4640      4650      4660 
               
               
                 BH 5   AGCAGTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTGGGGGGTA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: 
               
               
                 Licuw, AGCAGTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTGGGGGATA 
               
               
                        4760      4770      4780      4790      4800      4810 
               
               
                   
               
               
                             4670      4680      4690      4700      4710      4720 
               
               
                 BH 5   CAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAATTACA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::: 
               
               
                 Licuw, CAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAACTACA 
               
               
                        4820      4830      4840      4850      4860      4870 
               
               
                   
               
               
                             4730      4740      4750      4760      4770      4780 
               
               
                 BH 5   AAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTACAGGGACAGCAGAAATCCACT 
               
               
                        ::: ::::::::::::::::::::::::::::::::::::::::::: :: : :::: :: 
               
               
                 Licuw, AAAGCAAATTACAAAAATTCAAAATTTTCGGGTTTATTACAGGGACAACAAAGATCCCCT 
               
               
                        4880      4890      4900      4910      4920      4930 
               
               
                   
               
               
                             4790      4800      4810      4820      4830      4840 
               
               
                 BH 5   TTGGAAAGGACCAGCAAAGCTCCTCTGGAAAGGTGAAGGGGCAGTAGTAATACAAGATAA 
               
               
                        ::::::::::::::::::::: ::::: :::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TTGGAAAGGACCAGCAAAGCTTCTCTGCAAAGGTGAAGGGGCAGTAGTAATACAAGATAA 
               
               
                        4940      4950      4960      4970      4980      4990 
               
               
                   
               
               
                             4850      4860      4870      4880      4890      4900 
               
               
                 BH 5   TAGTGACATAAAAGTAGTGCCAAGAAGAAAAGCAAAGATCATTAGGGATTATGGAAAACA 
               
               
                        :::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::: 
               
               
                 Licuw, TAGTGACATAAAAGTAGTGCCAAGAAGAAAAGCAAAAATCATTAGGGATTATGGAAAACA 
               
               
                        5000      5010      5020      5030      5040      5050 
               
               
                   
               
               
                             4910      4920      4930      4940      4950      4960 
               
               
                 BH 5   GATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAGGATTAGAACATGGAAAAGTT 
               
               
                        :::::::::::::::::::::   :::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GATGGCAGGTGATGATTGTGT---AAGTAGACAGGATGAGGATTAGAACATGGAAAAGTT 
               
               
                        5060      5070         5080      5090      5100      5110 
               
               
                   
               
               
                             4970      4980      4990      5000      5010      5020 
               
               
                 BH 5   TAGTAAAACACCATATGTATGTTTCAGGGAAAGCTAGGGGATGGTTTTATAGACATCACT 
               
               
                        :::::::::::::::::::: :::::  ::::::::  :::::::::: ::: ::::::: 
               
               
                 Licuw, TAGTAAAACACCATATGTATATTTCAAAGAAAGCTAAAGGATGGTTTT-TAG-CATCACT 
               
               
                           5120      5130      5140      5150      5160        5170 
               
               
                   
               
               
                             5030      5040      5050      5060      5070      5080 
               
               
                 BH 5   ATGAAAGCCCTCATCCAAGAATAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGAT 
               
               
                         ::::::  ::::::::::: :::::::::::::::::::::: ::::::::::::: :: 
               
               
                 Licuw, -TGAAAGTACTCATCCAAGAGTAAGTTCAGAAGTACACATCCCCCTAGGGGATGCTAAAT 
               
               
                              5180      5190      5200      5210      5220      5230 
               
               
                   
               
               
                             5090      5100      5110       5120      5130      5140 
               
               
                 BH 5   TGGTAATAACAACATATTGGGGTCTGCATACA-GGAGAAAGAGACTGGCATTTGGGTCAG 
               
               
                        :::::::::::::::::::::::::::::::: ::::::::::: ::::::::::: ::: 
               
               
                 Licuw, TGGTAATAACAACATATTGGGGTCTGCATACAAGGAGAAAGAGAATGGCATTTGGG-CAG 
               
               
                              5240      5250      5260      5270      5280 
               
               
                   
               
               
                              5150      5160      5170      5180      5190      5200 
               
               
                 BH 5   GGAGTCTCCATAGAATGGAGGAAAAGGAGATATAGCACACAAGTAGACCCTGAACTAGCA 
               
               
                        :: ::: ::::::::::::: :::    ::  ::::::::::::::::::::  :::::: 
               
               
                 Licuw, GG-GTCGCCATAGAATGGAGAAAA----GAATTAGCACACAAGTAGACCCTGGCCTAGCA 
               
               
                     5290       5300      5310          5320      5330      5340 
               
               
                   
               
               
                              5210      5220      5230      5240      5250      5260 
               
               
                 BH 5   GACCAACTAATTCATCTGTATTACTTTGATTGTTTTTCAGACTCTGCTATAAGAAAGGCC 
               
               
                        :::::::::::::::::: :::: ::::::::::::::::: :::::::::: ::: ::: 
               
               
                 Licuw, GACCAACTAATTCATCTGCATTATTTTGATTGTTTTTCAGAATCTGCTATAAAAAATGCC 
               
               
                          5350      5360      5370      5380      5390      5400 
               
               
                   
               
               
                              5270      5280      5290      5300      5310      5320 
               
               
                 BH 5   TTATTAGGACACATAGTTAGCCCTAGGTGTGAATATCAAGCAGGACATAACAAGGTAGGA 
               
               
                         :::::::: : : :::::: ::::: ::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ATATTAGGATATAGAGTTAGTCCTAGCTGTGAATATCAAGCAGGACATAACAAGGTAGGA 
               
               
                          5410      5420      5430      5440      5450      5460 
               
               
                   
               
               
                              5330      5340      5350      5360      5370      5380 
               
               
                 BH 5   TCTCTACAATACTTGGCACTAGCAGCATTAATAACACCAAAAAAGGGAAAGCCACCTTTG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::  ::::::::::::: 
               
               
                 Licuw, TCTCTACAATACTTGGCACTAGCAGCATTAATAACACCAAAAAAGACAAAGCCACCTTTG 
               
               
                          5470      5480      5490      5500      5510      5520 
               
               
                   
               
               
                              5390      5400      5410      5420      5430      5440 
               
               
                 BH 5   CCTAGTGTTACGAAACTGACAGAGGATAGATGGAACAAGCCCCAGAAGACCAAGGGCCAC 
               
               
                        :::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CCTAGTGTTAAGAAACTGACAGAGGATAGATGGAACAAGCCCCAGAAGACCAAGGGCCAC 
               
               
                          5530      5540      5550      5560      5570      5580 
               
               
                   
               
               
                              5450      5460      5470      5480      5490      5500 
               
               
                 BH 5   AGAGGGAGCCACACAATGAATGGACACTAGAGCTTTTAGAGGAGCTTAAGAATGAAGCTG 
               
               
                        ::::::::::: :::::::::::::::::::::::::::::::::::::::  ::::::: 
               
               
                 Licuw, AGAGGGAGCCATACAATGAATGGACACTAGAGCTTTTAGAGGAGCTTAAGAGAGAAGCTG 
               
               
                          5590      5600      5610      5620      5630      5640 
               
               
                   
               
               
                              5510      5520      5530      5540      5550      5560 
               
               
                 BH 5   TTAGACATTTTCCTAGGATTTGGCTCCATGGCTTAGGGCAACATATCTATGAAACTTATG 
               
               
                        :::::::::::::::::   ::::::::: ::::::: ::: :::::::::::::::::: 
               
               
                 Licuw, TTAGACATTTTCCTAGGCCATGGCTCCATAGCTTAGGACAATATATCTATGAAACTTATG 
               
               
                          5650      5660      5670      5680      5690      5700 
               
               
                   
               
               
                              5570      5580      5590      5600      5610      5620 
               
               
                 BH 5   GGGATACTTGGGCAGGAGTGGAAGCCATAATAAGAATTCTGCAACAACTGCTGTTTATCC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: : 
               
               
                 Licuw, GGGATACTTGGGCAGGAGTGGAAGCCATAATAAGAATTCTGCAACAACTGCTGTTTATTC 
               
               
                          5710      5720      5730      5740      5750      5760 
               
               
                   
               
               
                              5630      5640      5650      5660      5670      5680 
               
               
                 BH 5   ATTTTCAGAATTGGGTGTCGACATAGCAGAATAGGCGTTACTCAACAGAGGAGAGCAAGA 
               
               
                        :::: :::::::::::::: :::::::::::::::: ::: ::::::::::::::::::: 
               
               
                 Licuw, ATTT-CAGAATTGGGTGTCAACATAGCAGAATAGGCATTATTCAACAGAGGAGAGCAAGA 
               
               
                           5770      5780      5790      5800      5810      5820 
               
               
                   
               
               
                                 5690      5700      5710      5720      5730 
               
               
                 BH 5   A---ATGGAGCCAGTAGATCCTAGACTAGAGCCCTGGAAGCATCCAGGAAGTCAGCCTAA 
               
               
                        :   :::::::::::::::::::  :::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AGAAATGGAGCCAGTAGATCCTAATCTAGAGCCCTGGAAGCATCCAGGAAGTCAGCCTAG 
               
               
                           5830      5840      5850      5860      5870      5880 
               
               
                   
               
               
                       5740      5750      5760      5770      5780      5790 
               
               
                 BH 5   AACTGCTTGTACCACTTGCTATTGTAAAAAGTGTTGCTTTCATTGCCAAGTTTGTTTCAT 
               
               
                         :::::::::: :: ::::::::::::::::::::::::::::::: : :  ::::::: 
               
               
                 Licuw, GACTGCTTGTAACAATTGCTATTGTAAAAAGTGTTGCTTTCATTGCTACGCGTGTTTCAC 
               
               
                           5890      5900      5910      5920      5930      5940 
               
               
                   
               
               
                       5800      5810      5820      5830      5840      5850 
               
               
                 BH 5   AACAAAAGCCTTAGGCATCTCCTATGGCAGGAAGAAGCGGAGACAGCGACGAA GAGCTC   
               
               
                        :: ::::: :::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AAGAAAAGGCTTAGGCATCTCCTATGGCAGGAAGAAGCGGAGACAGCGACGAAGAGCTC 
               
               
                           5950      5960      5970      5980      5990      6000 
               
               
                   
               
               
                        5860      5870      5880      5890      5900      5910 
               
               
                 BH 8  GAGCTCATCGAAGCAGTCAGACTCATCAAGTTTCTCTATCAAAGCAGTAAGTAGTACATGT 
               
               
                          ::::  ::  ::::::::::::::::: ::::::::::::::::::::::::: :::: 
               
               
                 Licuw,---CTCAG-GA--CAGTCAGACTCATCAAGCTTCTCTATCAAAGCAGTAAGTAGTAAATGT 
               
               
                                  6010      6020      6030      6040      6050 
               
               
                   
               
               
                       5920      5930         5940      5950      5960      5970 
               
               
                 BH 8   AATGCAACCTATACAAATA---GCAATAGTAGCATTAGTAGTAGCAATAATAATAGCAAT 
               
               
                        ::::::: :: ::::::::   ::::::::: :::::::::::: :  :::::::::::: 
               
               
                 Licuw, AATGCAATCTTTACAAATATTAGCAATAGTATCATTAGTAGTAGTAGCAATAATAGCAAT 
               
               
                       6060      6070      6080      6090      6100      6110 
               
               
                   
               
               
                          5980      5990      6000      6010      6020      6030 
               
               
                 BH 8   AGTTGTGTGGTCCATAGTAATCATAGAATATAGGAAAATATTAAGACAAAGAAAAATAGA 
               
               
                        :::::::::: :::::::: ::::::::::::::::::::::::::::::::::: :::: 
               
               
                 Licuw, AGTTGTGTGGACCATAGTACTCATAGAATATAGGAAAATATTAAGACAAAGAAAA-TAGA 
               
               
                       6120      6130      6140      6150      6160      6170 
               
               
                   
               
               
                          6040      6050      6060      6070      6080      6090 
               
               
                 BH 8   CAGGTTAATTGATAGACTAATAGAAAGAGCAGAAGACAGTGGCAATGAGAGTGAAGGAGA 
               
               
                        ::: :::::::::::: ::: ::::: ::::::::::::::::::::: :::::::: :: 
               
               
                 Licuw, CAGATTAATTGATAGAATAAGAGAAAAAGCAGAAGACAGTGGCAATGAAAGTGAAGGGGA 
               
               
                        6180      6190      6200      6210      6220      6230 
               
               
                   
               
               
                                   6100      6110      6120      6130      6140 
               
               
                 BH 8   ---------AATATCAGCACTTGTGGAGATGGGGGTGGAGATGGGGCACCATGCTCCTTG 
               
               
                                 : ::::::::::::::::::::::            ::::: ::::::::: 
               
               
                 Licuw, CCAGGAGGAATTATCAGCACTTGTGGAGATGGG------------GCACCTTGCTCCTTG 
               
               
                        6240      6250      6260                  6270      6280 
               
               
                   
               
               
                         6150      6160      6170      6180      6190      6200 
               
               
                 BH 8   GGATGTTGATGATCTGTAGTGCTACAGAAAAATTGTGGGTCACAGTCTATTTAGGGGTAC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::: ::::  :: :::: 
               
               
                 Licuw, GGATGTTGATGATCTGTAGTGCTACAGAAAAATTGTGGGTCACAGTTTATTATGGAGTAC 
               
               
                          6290      6300      6310      6320      6330      6340 
               
               
                   
               
               
                         6210      6220      6230      6240      6250      6260 
               
               
                 BH 8   CTGTGTGGAAGGAAGCAACCACCACTCTATTTTGTGCATCAGATGCTAAAGCATATGATA 
               
               
                        :::::::::: :::::::: :::::::::::::::::::::::::::: ::::::::::: 
               
               
                 Licuw, CTGTGTGGAAAGAAGCAACTACCACTCTATTTTGTGCATCAGATGCTAGAGCATATGATA 
               
               
                          6350      6360      6370      6380      6390      6400 
               
               
                   
               
               
                         6270      6280      6290      6300      6310      6320 
               
               
                 BH 8   CAGAGGTACATAATGTTTGGGCCACACATGCCTGTGTACCCACAGACCCCAACCCACAAG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CAGAGGTACATAATGTTTGGGCCACACATGCCTGTGTACCCACAGACCCCAACCCACAAG 
               
               
                          6410      6420      6430      6440      6450      6460 
               
               
                   
               
               
                         6330      6340      6350      6360      6370      6380 
               
               
                 BH 8   AAGTAGTATTGGTAAATGTGACAGAAAATTTTAACATGTGGAAAAATGACATGGTAGAAC 
               
               
                        :::::::::::: :::::::::::::::::::::::::::::::::: :::::::::::: 
               
               
                 Licuw, AAGTAGTATTGGGAAATGTGACAGAAAATTTTAACATGTGGAAAAATAACATGGTAGAAC 
               
               
                          6470      6480      6490      6500      6510      6520 
               
               
                   
               
               
                         6390      6400      6410      6420      6430      6440 
               
               
                 BH 8   AGATGCATGAGGATATAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAATTAA 
               
               
                        ::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AGATGCAGGAGGATATAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAATTAA 
               
               
                          6530      6540      6550      6560      6570      6580 
               
               
                   
               
               
                         6450      6460      6470      6480      6490      6500 
               
               
                 BH 8   CCCCACTCTGTGTTAGTTTAAAGTGCACTGATTTGAAGAATGATACTAATACCAATAGTA 
               
               
                        ::::::::::::::: :::::: ::::::::::::  ::: : ::::::::::::::::: 
               
               
                 Licuw, CCCCACTCTGTGTTACTTTAAATTGCACTGATTTGGGGAAGGCTACTAATACCAATAGTA 
               
               
                          6590      6600      6610      6620      6630      6640 
               
               
                   
               
               
                         6510      6520      6530      6540      6550      6560 
               
               
                 BH 8   GTAGCGGGAGAATGATAATGGAGAAAGGAGAGATAAAAAACTGCTCTTTCAATATCAGCA 
               
               
                        :::   ::: :     :::    :::::::: ::::::::::::::::::::::::: :: 
               
               
                 Licuw, GTAATTGGAAAGAAGAAATA---AAAGGAGAAATAAAAAACTGCTCTTTCAATATCACCA 
               
               
                          6650      6660         6670      6680      6690      6700 
               
               
                   
               
               
                         6570      6580      6590      6600      6610      6620 
               
               
                 BH 8   CAAGCAAAAGAGGTAAGGTGCAGAAAGAATATGCATTTTTTTATAAACTTGATATAATAC 
               
               
                        :::::: ::::: :::: : ::::::::: ::::: :::::  ::: :::::: :: ::: 
               
               
                 Licuw, CAAGCATAAGAGATAAGATTCAGAAAGAAAATGCACTTTTTCGTAACCTTGATGTAGTAC 
               
               
                             6710      6720      6730      6740      6750      6760 
               
               
                   
               
               
                         6630      6640            6650               6660      6670 
               
               
                 BH 8   CAATAGATAATGATA------CTACCAGCTATAC---------GTTGACAAGTTGTAACA 
               
               
                        :::::::::::: ::      :::::: ::::::         ::::: :  :::::::: 
               
               
                 Licuw, CAATAGATAATGCTAGTACTACTACCAACTATACCAACTATAGGTTGATACATTGTAACA 
               
               
                             6770      6780      6790      6800      6810      6820 
               
               
                   
               
               
                              6680      6690      6700      6710      6720      6730 
               
               
                 BH 8   CCTCAGTCATTACACAGGCCTGTCCAAAGGTATCCTTTGAGCCAATTCCCATACATTATT 
               
               
                          :::::::::::::::::::::::::::::::: ::::::::::::::::::::::::: 
               
               
                 Licuw, GATCAGTCATTACACAGGCCTGTCCAAAGGTATCATTTGAGCCAATTCCCATACATTATT 
               
               
                             6830      6840      6850      6860      6870      6880 
               
               
                   
               
               
                              6740      6750      6760      6770      6780      6790 
               
               
                 BH 8   GTGCCCCGGCTGGTTTTGCGATTCTAAAATGTAATAATAAGACGTTCAATGGAACAGGAC 
               
               
                        :: ::::::::::::::::::::::::: ::::::::::: ::::::::::::: ::::: 
               
               
                 Licuw, GTACCCCGGCTGGTTTTGCGATTCTAAAGTGTAATAATAAAACGTTCAATGGAAAAGGAC 
               
               
                             6890      6900      6910      6920      6930      6940 
               
               
                   
               
               
                              6800      6810      6820      6830      6840      6850 
               
               
                 BH 8   CATGTACAAATGTCAGCACAGTACAATGTACACATGGAATTAGGCCAGTAGTATCAACTC 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::: :::: ::::::: 
               
               
                 Licuw, CATGTACAAATGTCAGCACAGTACAATGTACACATGGAATTAGGCCAATAGTGTCAACTC 
               
               
                             6950      6960      6970      6980      6990      7000 
               
               
                   
               
               
                               6860      6870      6880      6890      6900 
               
               
                 BH 8   AACTG-CTGTTAAATGGCAGTCTAGCAGAAGAAGAGGTAGTAATTAGATCTGTCAATTTC 
               
               
                        ::::: :::::::::::::::::::::::::::::::::::::::::::::: ::::::: 
               
               
                 Licuw, AACTGTCTGTTAAATGGCAGTCTAGCAGAAGAAGAGGTAGTAATTAGATCTGACAATTTC 
               
               
                             7010      7020      7030      7040      7050      7060 
               
               
                   
               
               
                     6910      6920      6930      6940      6950      6960 
               
               
                 BH 8   ACGGACAATGCTAAAACCATAATAGTACAGCTGAACACATCTGTAGAAATTAATTGTACA 
               
               
                        ::: :::::::::::::::::::::::::::::::   :::::::: :::::: :::::: 
               
               
                 Licuw, ACGAACAATGCTAAAACCATAATAGTACAGCTGAATGAATCTGTAGCAATTAACTGTACA 
               
               
                             7070      7080      7090      7100      7110      7120 
               
               
                   
               
               
                     6970      6980      6990      7000      7010      7020 
               
               
                 BH 8   AGACCCAACAACAATACAAGAAAAAGTATCCAAATCCAGAGGGGACCAGGGAGAGCATTT 
               
               
                        :::::::::::::::::::::::::::::: : ::       :::::::::::::::::: 
               
               
                 Licuw, AGACCCAACAACAATACAAGAAAAAGTATCTATATA------GGACCAGGGAGAGCATTT 
               
               
                             7130      7140      7150            7160      7170 
               
               
                   
               
               
                     7030      7040         7050      7060      7070      7080 
               
               
                 BH 8   GTTACAATAGGAAAAATA---GGAAATATGAGACAAGCACATTGTAACATTAGTAGAGCA 
               
               
                          ::::: ::::: ::::   ::: :::: ::: :::::::::::::::::::::::::: 
               
               
                 Licuw, CATACAACAGGAAGAATAATAGGAGATATAAGAAAAGCACATTGTAACATTAGTAGAGCA 
               
               
                         7180      7190      7200      7210      7220      7230 
               
               
                   
               
               
                        7090      7100      7110      7120      7130      7140 
               
               
                 BH 8   AAATGGAATGCCACTTTAAAACAGATAGATAGCAAATTAAGAGAACAATTTGGAAATAAT 
               
               
                         ::::::::  ::::::: ::::::::: ::  :::::::::::::: ::::: :::::: 
               
               
                 Licuw, CAATGGAATAACACTTTAGAACAGATAGTTAAAAAATTAAGAGAACAGTTTGGGAATAAT 
               
               
                         7240      7250      7260      7270      7280      7290 
               
               
                   
               
               
                        7150      7160      7170      7180      7190      7200 
               
               
                 BH 8   AAAACAATAATCTTTAAGCAGTCCTCAGGAGGGGACCCAGAAATTGTAACGCACAGTTTT 
               
               
                        ::::::::: ::::::: :: :::::::::::::::::::::::::::: :::::::::: 
               
               
                 Licuw, AAAACAATAGTCTTTAATCAATCCTCAGGAGGGGACCCAGAAATTGTAATGCACAGTTTT 
               
               
                         7300      7310      7320      7330      7340      7350 
               
               
                   
               
               
                        7210      7220      7230      7240      7250      7260 
               
               
                 BH 8   AATTGTGGAGGGGAATTTTTCTACTGTAATTCAACACAACTGTTTAATAGTACTTGGAGT 
               
               
                        :::::: ::::::::::::::::::::::: :::::::::::::::::: ::: ::::: 
               
               
                 Licuw, AATTGTAGAGGGGAATTTTTCTACTGTAATACAACACAACTGTTTAATAATACATGGAG- 
               
               
                         7360      7370      7380      7390      7400      7410 
               
               
                   
               
               
                        7270      7280      7290               7300      7310 
               
               
                 BH 8   ACTAAAGGGTCAAATAACACTGAAGGAAGT---------GACACAATCACCCTCCCATGC 
               
               
                                :: :::: :::::::::::: :         ::::::::::  :::::::: 
               
               
                 Licuw, --------GTTAAATCACACTGAAGGAACTAAAGGAAATGACACAATCATACTCCCATGT 
               
               
                                  7420      7430      7440      7450      7460 
               
               
                   
               
               
                       7320      7330      7340      7350      7360      7370 
               
               
                 BH 8   AGAATAAAACAAATTATAAACATGTGGCAGGAAGTAGGAAAAGCAATGTATGCCCCTCCC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AGAATAAAACAAATTATAAACATGTGGCAGGAAGTAGGAAAAGCAATGTATGCCCCTCCC 
               
               
                        7470      7480      7490      7500      7510      7520 
               
               
                   
               
               
                       7380      7390      7400      7410      7420      7430 
               
               
                 BH 8   ATCAGTGGACAAATTAGATGTTCATCAAATATTACAGGGCTGCTATTAACAAGAGATGGT 
               
               
                        ::  : ::::::::::: :::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ATTGGAGGACAAATTAGTTGTTCATCAAATATTACAGGGCTGCTATTAACAAGAGATGGT 
               
               
                        7530      7540      7550      7560      7570      7580 
               
               
                   
               
               
                       7440         7450      7460      7470      7480      7490 
               
               
                 BH 8   GGTAATA--GCAAC-AATGAGTCCGAGATCTTCAGACCTGGAGGAGGAGATATGAGGGAC 
               
               
                        ::::  :  : ::: :::::  ::::: :::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GGTACAAATGTAACTAATGACACCGAGGTCTTCAGACCTGGAGGAGGAGATATGAGGGAC 
               
               
                        7590      7600      7610      7620      7630      7640 
               
               
                   
               
               
                          7500      7510      7520      7530      7540      7550 
               
               
                 BH 8   AATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCA 
               
               
                        ::::::::::::::::::::::::::::::::: :::::::::::::::::::: ::::: 
               
               
                 Licuw, AATTGGAGAAGTGAATTATATAAATATAAAGTAATAAAAATTGAACCATTAGGAATAGCA 
               
               
                        7650      7660      7670      7680      7690      7700 
               
               
                   
               
               
                          7560      7570      7580      7590      7600         7610 
               
               
                 BH 8    CCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAG---GA 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::   :: 
               
               
                 Licuw, CCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGTAGGA 
               
               
                        7710      7720      7730      7740      7750      7760 
               
               
                   
               
               
                             7620      7630      7640      7650      7660      7670 
               
               
                 BH 8   GCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACG 
               
               
                        ::: :::::::::::::::::::::::::::::::::::::::: :::: :::: ::::: 
               
               
                 Licuw, GCTATGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGGGCAGTGTCATTGACG 
               
               
                        7770      7780      7790      7800      7810      7820 
               
               
                   
               
               
                             7680      7690      7700      7710      7720      7730 
               
               
                 BH 8   CTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTG 
               
               
                        ::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::: 
               
               
                 Licuw, CTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAACAGCAGAACAATTTGCTG 
               
               
                        7830      7840      7850      7860      7870      7880 
               
               
                   
               
               
                             7740      7750      7760      7770      7780       7790 
               
               
                 BH 8   AGGGCTATTGAGGGCCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCA-GCAGCTC 
               
               
                        :::::::::::::  ::::: ::::::::::::::::::::::::::::::: ::::::: 
               
               
                 Licuw, AGGGCTATTGAGGCGCAACAACATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTC 
               
               
                        7890      7900      7910      7920      7930      7940 
               
               
                   
               
               
                              7800      7810      7820      7830      7840      7850 
               
               
                 BH 8   CAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGG 
               
               
                        ::::::::: :::::::::::::::::::::::: :::::::::::::::   ::::::: 
               
               
                 Licuw, CAGGCAAGAGTCCTGGCTGTGGAAAGATACCTAAGGGATCAACAGCTCCTA--GATTTGG 
               
               
                        7950      7960      7970      7980      7990        8000 
               
               
                   
               
               
                              7860      7870      7880      7890      7900      7910 
               
               
                 BH 8   GGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAAT 
               
               
                        ::: :::::::::: ::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GGT-GCTCTGGAAA-CTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAAT 
               
               
                           8010       8020      8030      8040      8050      8060 
               
               
                   
               
               
                              7920      7930      7940      7950      7960      7970 
               
               
                 BH 8   AAATCTCTGGAACAGATTTGGAATAACATGACCTGGATGGAGTGGGACAGAGAAATTAAC 
               
               
                        :::::::::::: : :::::: ::::::::::::: ::: ::::::: ::::::::: :: 
               
               
                 Licuw, AAATCTCTGGAAGACATTTGGGATAACATGACCTG-ATGCAGTGGGAAAGAGAAATTGAC 
               
               
                            8070      8080      8090       8100      8110      8120 
               
               
                   
               
               
                              7980      7990      8000      8010      8020      8030 
               
               
                 BH 8   AATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAAT 
               
               
                        :::::::::: :  :::: :: ::::: ::::::::::::: ::::: :::::::::::: 
               
               
                 Licuw, AATTACACAAACACAATATACACCTTACTTGAAGAATCGCAGAACCAACAAGAAAAGAAT 
               
               
                             8130      8140      8150      8160      8170      8180 
               
               
                   
               
               
                              8040      8050      8060      8070      8080      8090 
               
               
                 BH 8   GAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACA 
               
               
                        :::::::::::::: ::::: ::::: ::::::::::::::::::::::::: ::::::: 
               
               
                 Licuw, GAACAAGAATTATTAGAATTGGATAAGTGGGCAAGTTTGTGGAATTGGTTTAGCATAACA 
               
               
                             8190      8200      8210      8220      8230      8240 
               
               
                   
               
               
                              8100      8110      8120      8130      8140      8150 
               
               
                 BH 8   AATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGA 
               
               
                        :: :::::::::::::::::  :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AACTGGCTGTGGTATATAAAGATATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGA 
               
               
                             8250      8260      8270      8280      8290      8300 
               
               
                   
               
               
                              8160      8170      8180      8190      8200      8210 
               
               
                 BH 8   ATAGTTTTTGCTGTACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCG 
               
               
                        :::::::::::::: :::::::::::::::::::::::::::::::: :::::::: :: 
               
               
                 Licuw, ATAGTTTTTGCTGTGCTTTCTATAGTGAATAGAGTTAGGCAGGGATACTCACCATTGTCA 
               
               
                             8310      8320      8330      8340      8350      8360 
               
               
                   
               
               
                              8220      8230      8240      8250      8260      8270 
               
               
                 BH 8   TTTCAGACCCACCTCCCAAACCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAA 
               
               
                        ::::::::::  ::::::   :::::   ::::::::: ::::: ::::: ::::::::: 
               
               
                 Licuw, TTTCAGACCCGGCTCCCAGTGCCGAG---ACCCGACAG-CCCGACGGAATCGAAGAAGAA 
               
               
                             8370      8380         8390       8400      8410 
               
               
                   
               
               
                              8280      8290      8300      8310      8320      8330 
               
               
                 BH 8   GGTGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCCTTAGCACTTATC 
               
               
                        :: :::::::::::::::::::::::: ::::::::::: : :::: ::::::::::::: 
               
               
                 Licuw, GG-GGAGAGAGAGACAGAGACAGATCCGTTCGATTAGTGGATGGATTCTTAGCACTTATC 
               
               
                        8420      8430      8440      8450      8460      8470 
               
               
                   
               
               
                              8340      8350      8360      8370      8380      8390 
               
               
                 BH 8   TGGGACGATCTGCGGAGCCTGTGCCTCTTCAGCTACCACCGCTTGAGAGACTTACTCTTG 
               
               
                        ::::: :::::::::::::::::::::::::::::::  :: :::::::::::::::::: 
               
               
                 Licuw, TGGGAAGATCTGCGGAGCCTGTGCCTCTTCAGCTACCGGCGGTTGAGAGACTTACTCTTG 
               
               
                        8480      8490      8500      8510      8520      8530 
               
               
                   
               
               
                              8400      8410      8420      8430      8440      8450 
               
               
                 BH 8   ATTGTAACGAGGATTGTGGAACTTCTGGGACGCAGGGGGTGGGAAGCCCTCAAATATTGG 
               
               
                        :::: : :::::: ::::::: ::::::: : :::::::::::::   :::::::::::: 
               
               
                 Licuw, ATTGCAGCGAGGACTGTGGAAATTCTGGGGCACAGGGGGTGGGAA---CTCAAATATTGG 
               
               
                        8540      8550      8560      8570      8580         8590 
               
               
                   
               
               
                              8460      8470      8480      8490      8500      8510 
               
               
                 BH 8   TGGAATCTCCTACAGTATTGGAGTCAGGAACTAAAGAATAGTGCTGTTAACTTGCTCAAT 
               
               
                        :::: :::::: :::::::::: :::::::::::::::::::::::::: :: :::::: 
               
               
                 Licuw, TGGAGTCTCCTGCAGTATTGGATTCAGGAACTAAAGAATAGTGCTGTTAGCTGGCTCAAC 
               
               
                           8600      8610      8620      8630      8640      8650 
               
               
                   
               
               
                              8520      8530      8540      8550      8560      8570 
               
               
                 BH 8   GCCACAGCCATAGCAGTAGCTGAGGGGACAGATAGGGTTATAGAAGTATTACAAGCAGCT 
               
               
                        :::::::: ::::::::: :::::::::::::::::::::::::::::  ::::  :::: 
               
               
                 Licuw, GCCACAGCTATAGCAGTAACTGAGGGGACAGATAGGGTTATAGAAGTAGCACAAAGAGCT 
               
               
                           8660      8670      8680      8690      8700      8710 
               
               
                   
               
               
                              8580      8590      8600      8610      8620      8630 
               
               
                 BH 8   TATAGAGCCATTCGCCACATACCTAGAAGAATAAGACAGGGCTTGGAAAGGATTTTGCTA 
               
               
                        :::::::: :::: :::::::: ::::::::: :::::   :::::::::: :::::::: 
               
               
                 Licuw, TATAGAGCTATTCTCCACATACATAGAAGAATTAGACA---CTTGGAAAGGCTTTTGCTA 
               
               
                           8720      8730      8740      8750         8760      8770 
               
               
                   
               
               
                              8640      8650      8660      8670      8680      8690 
               
               
                 BH 8   TAAGATGGGTGGCAAGTGGTCAAAAAGTAGTGTGGTTGGATGGCCTGCTGTAAGGGAAAG 
               
               
                        ::::::::: :   ::::::::::: : ::: :::  :::::: ::::: :::::::::: 
               
               
                 Licuw, TAAGATGGGGG---AGTGGTCAAAACG-AGTATGGG-GGATGGTCTGCTATAAGGGAAAG 
               
               
                              8780         8790       8800       8810      8820 
               
               
                   
               
               
                              8700                  8710      8720      8730 
               
               
                 BH 8   AATGAGACGAGCTGAGCCA------------GCAGCAGATGGGGTGGGAGCAGTATCTCG 
               
               
                        :::::::::::::::::::            :::::::::::::::::: :::::::::: 
               
               
                 Licuw, AATGAGACGAGCTGAGCCACGAGCTGAGCCAGCAGCAGATGGG-TGGGA-CAGTATCTCG 
               
               
                         8830      8840      8850      8860       8870       8880 
               
               
                   
               
               
                      8740      8750      8760      8770      8780      8790 
               
               
                 BH 8   AGACCTAGAAAAACATGGAGCAATCACAAGTAGCAATACAGCAGCTACCAATGCTGATTG 
               
               
                        :::::: ::::::::::::  ::::::::::::::::::::::::::: ::::::::::: 
               
               
                 Licuw, AGACCTGGAAAAACATGGA--AATCACAAGTAGCAATACAGCAGCTACTAATGCTGATTG 
               
               
                           8890      8900        8910      8920      8930      8940 
               
               
                   
               
               
                      8800      8810      8820      8830      8840      8850 
               
               
                 BH 8   TGCTTGGCTAGAAGCACAAGAGGAGGAGGAGGTGGGTTTTCCAGTCACACCTCAGGTACC 
               
               
                        ::: ::::::::::::::::::::::: ::::::::::::::::::: :::::::::::: 
               
               
                 Licuw, TGCCTGGCTAGAAGCACAAGAGGAGGAAGAGGTGGGTTTTCCAGTCAGACCTCAGGTACC 
               
               
                             8950      8960      8970      8980      8990      9000 
               
               
                   
               
               
                      8860      8870      8880      8890      8900      8910 
               
               
                 BH 8   TTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAGAAAAGGG 
               
               
                        :::::::::::::::::::::::::::: ::::: ::::::::::::::::::::::::: 
               
               
                 Licuw, TTTAAGACCAATGACTTACAAGGCAGCTTTAGATATTAGCCACTTTTTAAAAGAAAAGGG 
               
               
                             9010      9020      9030      9040      9050      9060 
               
               
                   
               
               
                      8920      8930      8940      8950      8960      8970 
               
               
                 BH 8   GGGACTGGAAGGGCTAATTCACTCCCAACGAAGACAAGATATCCTTGATCTGTGGATCCA 
               
               
                        :::::::::::::::::::   :::::: :::::::::: :::::::::::::::::: : 
               
               
                 Licuw, GGGACTGGAAGGGCTAATTTGGTCCCAAAGAAGACAAGAGATCCTTGATCTGTGGATCTA 
               
               
                             9070      9080      9090      9100      9110      9120 
               
               
                   
               
               
                      8980      8990      9000      9010      9020      9030 
               
               
                 BH 8   CCACACACAAGGCTACTTCCCTGATTGGCAGAACTACACACCAGGGCCAGGGATCAGATA 
               
               
                        ::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::: 
               
               
                 Licuw, CCACACACAAGGCTACTTCCCTGATTGGCAGAATTACACACCAGGGCCAGGGATCAGATA 
               
               
                             9130      9140      9150      9160      9170      9180 
               
               
                   
               
               
                      9040      9050      9060      9070      9080      9090 
               
               
                 BH 8   TCCACTGACCTTTGGATGGTGCTACAAGCTAGTACCAGTTGAGCCAGAGAAGATAGAAGA 
               
               
                        ::::::::::::::::::::::: :::::::::::::::::::::::::::: ::::::: 
               
               
                 Licuw, TCCACTGACCTTTGGATGGTGCTTCAAGCTAGTACCAGTTGAGCCAGAGAAGGTAGAAGA 
               
               
                             9190      9200      9210      9220      9230      9240 
               
               
                   
               
               
                      9100      9110      9120      9130      9140      9150 
               
               
                 BH 8   AGCCAATAAAGGAGAGAACACCAGCTTGTTACACCCTGTGAGCCTGCATGGGATGGATGA 
               
               
                         :::::: ::::::::::::  ::::::::::::::: ::::::::::::::::::: :: 
               
               
                 Licuw, GGCCAATGAAGGAGAGAACAA-AGCTTGTTACACCCTATGAGCCTGCATGGGATGGAGGA 
               
               
                             9250      9260       9270      9280      9290      9300 
               
               
                   
               
               
                      9160      9170      9180      9190      9200      9210 
               
               
                 BH 8   CCCTGAGAGAGAAGTGTTAGAGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACATGGC 
               
               
                        : : :::: ::::::::::: ::::::::::::::::   :::::::::::::::::::: 
               
               
                 Licuw, CGCGGAGAAAGAAGTGTTAGTGTGGAGGTTTGACAGCAAACTAGCATTTCATCACATGGC 
               
               
                              9310      9320      9330      9340      9350      9360 
               
               
                   
               
               
                      9220      9230      9240      9250      9260      9270 
               
               
                 BH 8   CCGAGAGCTGCATCCGGAGTACTTCAAGAACTGCTGACATCGAGCTTGCTACAAGGGACT 
               
               
                        ::::::::::::::::::::::: :::  :::::::::::::::::: :::::::::::: 
               
               
                 Licuw, CCGAGAGCTGCATCCGGAGTACTACAAAGACTGCTGACATCGAGCTTTCTACAAGGGACT 
               
               
                              9370      9380      9390      9400      9410      9420 
               
               
                   
               
               
                      9280      9290              9300      9310      9320      9330 
               
               
                 BH 8   TTCCGCTGGGGACTTT--------GCGTGGCCTGGGCGGGACTGGGGAGTGGCGAGCCCT 
               
               
                        ::::::::::::::::        ::::::::::::::::::::::::::::::  :::: 
               
               
                 Licuw, TTCCGCTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGT-CCCT 
               
               
                              9430      9440      9450      9460      9470 
               
               
                   
               
               
                              9340              9350      9360      9370      9380 
               
               
                 BH 8   CAGATCCTGCATATAA--------TTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGA 
               
               
                        ::::::::::::::::        :::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CAGATGCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGA 
               
               
                     9480      9490      9500      9510      9520      9530 
               
               
                   
               
               
                            9390 
               
               
                 BH 8   TCTGAGCCTGGGAGCTC------------------------------------------- 
               
               
                        ::::::::::::::::: 
               
               
                 Licuw, TCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCT 
               
               
                     9540      9550      9560      9570      9580      9590 
               
               
                   
               
               
                        ------------------------------------------------------------ 
               
               
                   
               
               
                 Licuw, TGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGAT 
               
               
                     9600      9610      9620      9630      9640      9650 
               
               
                   
               
               
                        ----------------------------------------- 
               
               
                   
               
               
                 Licuw, CCCTCAGACCCTTTTAGTCAGTGTGGAAAAATCTCTAGCAG 
               
               
                     9660      9670      9680      9690      9700  
               
               
                   
               
             
          
         
       
     
     
       
         
               
             
               
             
           
               
                 TABLE III 
               
               
                   
               
               
                  (BH 10 v. LUCIW) 
               
               
                 90.0% identity 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                                 10        20        30        40        50 
               
               
                 BH 10  -TGGAAGGGCTAATTCACTCCCAACGAAGACAAGATATCCTTGATCTGTGGATCTACCAC 
               
               
                         ::::::::::::::   :::::: :::::::::: :::::::::::::::::::::::: 
               
               
                 Licuw, CTGGAAGGGCTAATTTGGTCCCAAAGAAGACAAGAGATCCTTGATCTGTGGATCTACCAC 
               
               
                                10        20        30        40        50        60 
               
               
                   
               
               
                       60        70        80        90       100       110 
               
               
                 BH 10  ACACAAGGCTACTTCCCTGATTAGCAGAACTACACACCAGGGCCAGGGATCAGATATCCA 
               
               
                        :::::::::::::::::::::: :::::: :::::::::::::::::::::::::::::: 
               
               
                 Licuw, ACACAAGGCTACTTCCCTGATTGGCAGAATTACACACCAGGGCCAGGGATCAGATATCCA 
               
               
                                70        80        90       100       110       120 
               
               
                   
               
               
                      120       130       140       150       160       170 
               
               
                 BH 10  CTGACCTTTGGATGGTGCTACAAGCTAGTACCAGTTGAGCCAGAGAAGTTAGAAGAAGCC 
               
               
                        ::::::::::::::::::: :::::::::::::::::::::::::::: ::::::: ::: 
               
               
                 Licuw, CTGACCTTTGGATGGTGCTTCAAGCTAGTACCAGTTGAGCCAGAGAAGGTAGAAGAGGCC 
               
               
                               130       140       150       160       170       180 
               
               
                   
               
               
                      180       190       200       210       220       230 
               
               
                 BH 10  AACAAAGGAGAGAACACCAGCTTGTTACACCCTGTGAGCCTGCATGGAATGGATGACCCG 
               
               
                        ::  :::::::::::: :::::::::::::::: ::::::::::::: ::::: ::: :: 
               
               
                 Licuw, AATGAAGGAGAGAACAACAGCTTGTTACACCCTATGAGCCTGCATGGGATGGAGGACGCG 
               
               
                               190       200       210       220       230       240 
               
               
                   
               
               
                      240       250       260       270       280       290 
               
               
                 BH 10  GAGAGAGAAGTGTTAGAGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACATGGCCCGA 
               
               
                        :::: ::::::::::: ::::::::::::::::   :::::::::::::::::::::::: 
               
               
                 Licuw, GAGAAAGAAGTGTTAGTGTGGAGGTTTGACAGCAAACTAGCATTTCATCACATGGCCCGA 
               
               
                               250       260       270       280       290       300 
               
               
                   
               
               
                      300       310       320       330       340       350 
               
               
                 BH 10  GAGCTGCATCCGGAGTACTTCAAGAACTGCTGACATCGAGCTTGCTACAAGGGACTTTCC 
               
               
                        ::::::::::::::::::: :::  :::::::::::::::::: :::::::::::::::: 
               
               
                 Licuw, GAGCTGCATCCGGAGTACTACAAAGACTGCTGACATCGAGCTTTCTACAAGGGACTTTCC 
               
               
                               310       320       330       340       350       360 
               
               
                   
               
               
                      360       370       380       390       400       410 
               
               
                 BH 10  GCTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGAGCCCTCAGA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::  :::::::: 
               
               
                 Licuw, GCTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGT-CCCTCAGA 
               
               
                               370       380       390       400       410 
               
               
                   
               
               
                      420       430        440       450       460       470 
               
               
                 BH 10  TCCTGCATATAAGCAG-CTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCT 
               
               
                        : :::::::::::::: ::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TGCTGCATATAAGCAGACTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCT 
               
               
                      420       430       440       450       460       470 
               
               
                   
               
               
                       480       490 
               
               
                 BH 10  GAGCCTGGGAGCTC---------------------------------------------- 
               
               
                        :::::::::::::: 
               
               
                 Licuw, GAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGC 
               
               
                      480       490       500       510       520       530 
               
               
                   
               
               
                        ------------------------------------------------------------ 
               
               
                   
               
               
                 Licuw, CTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCC 
               
               
                      540       550       560       570       580       590 
               
               
                   
               
               
                        ------------------------------------------------------------ 
               
               
                   
               
               
                 Licuw, TCAGACCCTTTTAGTCAGTGTGGAAAAATCTCTAGCAGTGGCGCCCGAACAGGGACGCGA 
               
               
                      600       610       620       630       640       650 
               
               
                   
               
               
                                                 500       510       520       530 
               
               
                 BH 10  --------------------GAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCA 
               
               
                                            :::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AAGCGAAAGTAGAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCA 
               
               
                      660       670       680       690       700       710 
               
               
                   
               
               
                             540       550       560       570       580       590 
               
               
                 BH 10  CGGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCT 
               
               
                        : :::::::::::::::::::::::::::::::::::::   :::::::::::::::::: 
               
               
                 Licuw, CAGCAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAA--TTTTTGACTAGCGGAGGCT 
               
               
                      720       730       740       750         760       770 
               
               
                   
               
               
                             600         610       620       630       640       650 
               
               
                 BH 10  AGAAGGAGAGAGA--TGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGAT 
               
               
                        :::::::::::::  :::::::::::::::: ::::::::::::::::::::::::  :: 
               
               
                 Licuw, AGAAGGAGAGAGAGATGGGTGCGAGAGCGTCGGTATTAAGCGGGGGAGAATTAGATAAAT 
               
               
                        780       790       800       810       820       830 
               
               
                   
               
               
                               660       670       680       690       700       710 
               
               
                 BH 10  GGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTAT 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::: 
               
               
                 Licuw, GGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAGTTAAAACATATAGTAT 
               
               
                        840       850       860       870       880       890 
               
               
                   
               
               
                               720       730       740       750       760       770 
               
               
                 BH 10  GGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAG 
               
               
                        ::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::: 
               
               
                 Licuw, GGGCAAGCAGGGAGCTAGAACGATTCGCAGTCAATCCTGGCCTGTTAGAAACATCAGAAG 
               
               
                        900       910       920       930       940       950 
               
               
                   
               
               
                               780       790       800       810       820       830 
               
               
                 BH 10  GCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTA 
               
               
                        :::: ::::::::: ::::::::::::: ::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GCTGCAGACAAATATTGGGACAGCTACAGCCATCCCTTCAGACAGGATCAGAAGAACTTA 
               
               
                        960       970       980       990      1000      1010 
               
               
                   
               
               
                               840       850       860       870       880       890 
               
               
                 BH 10  GATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAG 
               
               
                 :::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::  :::::: 
               
               
                 Licuw, GATCATTATATAATACAGTAGCAACCCTCTATTGTGTACATCAAAGGATAGATGTAAAAG 
               
               
                       1020      1030      1040      1050      1060      1070 
               
               
                   
               
               
                               900       910       920       930       940       950 
               
               
                 BH 10  ACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGAAAAAAGCAC 
               
               
                        ::::::::::::::::::: ::::::::::::::::::::::::::::::::::: :::: 
               
               
                 Licuw, ACACCAAGGAAGCTTTAGAGAAGATAGAGGAAGAGCAAAACAAAAGTAAGAAAAAGGCAC 
               
               
                       1080      1090      1100      1110      1120      1130 
               
               
                   
               
               
                            960                970       980       990      1000 
               
               
                 BH 10  AGCAAGCAGCAGCTGA------CACAGGACACAGCAGTCAGGTCAGCCAAAATTACCCTA 
               
               
                        :::::::::::::::       ::::::: ::::::: :::::::::::::::::::::: 
               
               
                 Licuw, AGCAAGCAGCAGCTGCAGCTGGCACAGGAAACAGCAGCCAGGTCAGCCAAAATTACCCTA 
               
               
                       1140      1150      1160      1170      1180      1190 
               
               
                   
               
               
                          1010      1020      1030      1040      1050      1060 
               
               
                 BH 10  TAGTGCAGAACATCCAGGGGCAAATGGTACATCAGGCCATATCACCTAGAACTTTAAATG 
               
               
                        ::::::::::: : :::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAGTGCAGAACCTACAGGGGCAAATGGTACATCAGGCCATATCACCTAGAACTTTAAATG 
               
               
                       1200      1210      1220      1230      1240      1250 
               
               
                   
               
               
                          1070      1080      1090      1100      1110      1120 
               
               
                 BH 10  CATGGGTAAAAGTAGTAGAAGAGAAGGCTTTCAGCCCAGAAGTAATACCCATGTTTTCAG 
               
               
                        :::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CATGGGTAAAAGTAGTAGAAGAAAAGGCTTTCAGCCCAGAAGTAATACCCATGTTTTCAG 
               
               
                       1260      1270      1280      1290      1300      1310 
               
               
                   
               
               
                          1130      1140      1150      1160      1170      1180 
               
               
                 BH 10  CATTATCAGAAGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGAC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CATTATCAGAAGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGAC 
               
               
                       1320      1330      1340      1350      1360      1370 
               
               
                   
               
               
                          1190      1200      1210      1220      1230      1240 
               
               
                 BH 10  ATCAAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGAATGGGATA 
               
               
                        ::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::: 
               
               
                 Licuw, ATCAAGCAGCCATGCAAATGTTAAAAGAGACTATCAATGAGGAAGCTGCAGAATGGGATA 
               
               
                       1380      1390      1400      1410      1420      1430 
               
               
                   
               
               
                          1250      1260      1270      1280      1290      1300 
               
               
                 BH 10  GAGTACATCCAGTGCATGCAGGGCCTATTGCACCAGGCCAGATGAGAGAACCAAGGGGAA 
               
               
                        :::: ::::::::::::::::::::::::::::::::::: ::::::::::::::::::: 
               
               
                 Licuw, GAGTGCATCCAGTGCATGCAGGGCCTATTGCACCAGGCCAAATGAGAGAACCAAGGGGAA 
               
               
                       1440      1450      1460      1470      1480      1490 
               
               
                   
               
               
                          1310      1320      1330      1340      1350      1360 
               
               
                 BH 10  GTGACATAGCAGGAACTACTAGTACCCTTCAGGAACAAATAGGATGGATGACAAATAATC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GTGACATAGCAGGAACTACTAGTACCCTTCAGGAACAAATAGGATGGATGACAAATAATC 
               
               
                       1500      1510      1520      1530      1540      1550 
               
               
                   
               
               
                          1370      1380      1390      1400      1410      1420 
               
               
                 BH 10  CACCTATCCCAGTAGGAGAAATTTATAAAAGATGGATAATCCTGGGATTAAATAAAATAG 
               
               
                        :::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CACCTATCCCAGTAGGAGAAATCTATAAAAGATGGATAATCCTGGGATTAAATAAAATAG 
               
               
                       1560      1570      1580      1590      1600      1610 
               
               
                   
               
               
                          1430      1440      1450      1460      1470      1480 
               
               
                 BH 10  TAAGAATGTATAGCCCTACCAGCATTCTGGACATAAGACAAGGACCAAAAGAACCTTTTA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::: ::::: :::: 
               
               
                 Licuw, TAAGAATGTATAGCCCTACCAGCATTCTGGACATAAGACAAGGACCAAAGGAACCCTTTA 
               
               
                       1620      1630      1640      1650      1660      1670 
               
               
                   
               
               
                          1490      1500      1510      1520      1530      1540 
               
               
                 BH 10  GAGACTATGTAGACCGGTTCTATAAAACTCTAAGAGCCGAGCAAGCTTCACAGGAGGTAA 
               
               
                        :::: ::::::::::::::::::::::::::::::::::: :::::::::::::: :::: 
               
               
                 Licuw, GAGATTATGTAGACCGGTTCTATAAAACTCTAAGAGCCGAACAAGCTTCACAGGATGTAA 
               
               
                       1680      1690      1700      1710      1720      1730 
               
               
                   
               
               
                          1550      1560      1570      1580      1590      1600 
               
               
                 BH 10  AAAATTGGATGACAGAAACCTTGTTGGTCCAAAATGCGAACCCAGATTGTAAGACTATTT 
               
               
                        ::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::: 
               
               
                 Licuw, AAAATTGGATGACAGAAACCTTGTTGGTCCAAAATGCAAACCCAGATTGTAAGACTATTT 
               
               
                       1740      1750      1760      1770      1780      1790 
               
               
                   
               
               
                          1610      1620      1630      1640      1650      1660 
               
               
                 BH 10  TAAAAGCATTGGGACCAGCGGCTACACTAGAAGAAATGATGACAGCATGTCAGGGAGTAG 
               
               
                        ::::::::::::::::::: :::::::::::::::::::::::::::::::::::::: : 
               
               
                 Licuw, TAAAAGCATTGGGACCAGCAGCTACACTAGAAGAAATGATGACAGCATGTCAGGGAGTGG 
               
               
                       1800      1810      1820      1830      1840      1850 
               
               
                   
               
               
                          1670      1680      1690      1700      1710      1720 
               
               
                 BH 10  GAGGACCCGGCCATAAGGCAAGAGTTTTGGCTGAAGCAATGAGCCAAGTAACAAATACAG 
               
               
                        : :::::::::::::: :::::::::::::::::::: :::::::::::::::::: ::: 
               
               
                 Licuw, GGGGACCCGGCCATAAAGCAAGAGTTTTGGCTGAAGCCATGAGCCAAGTAACAAATCCAG 
               
               
                       1860      1870      1880      1890      1900      1910 
               
               
                   
               
               
                          1730      1740      1750      1760      1770      1780 
               
               
                 BH 10  CTACCATAATGATGCAGAGAGGCAATTTTAGGAACCAAAGAAAGATGGTTAAGTGTTTCA 
               
               
                        ::: :::::::::::::::::::::::::::::::::::::::::  ::::::::::::: 
               
               
                 Licuw, CTAACATAATGATGCAGAGAGGCAATTTTAGGAACCAAAGAAAGACTGTTAAGTGTTTCA 
               
               
                       1920      1930      1940      1950      1960      1970 
               
               
                   
               
               
                          1790      1800      1810      1820      1830      1840 
               
               
                 BH 10  ATTGTGGCAAAGAAGGGCACACAGCCAGAAATTGCAGGGCCCCTAGGAAAAAGGGCTGTT 
               
               
                        :::::::::::::::: :::: ::::: :::::::::::::::::::::::::::   :: 
               
               
                 Licuw, ATTGTGGCAAAGAAGG-CACATAGCCAAAAATTGCAGGGCCCCTAGGAAAAAGGG---TT 
               
               
                       1980      1990       2000      2010      2020      2030 
               
               
                   
               
               
                          1850      1860      1870      1880      1890      1900 
               
               
                 BH 10  GGAAATGTGGAAAGGAAGGACACCAAATGAAAGATTGTACTGAGAGACAGGCTAATTTTT 
               
               
                         : : ::::::: :::::::::::::::::::::::: :::::::::::::::::::::: 
               
               
                 Licuw, TGGAGTGTGGAAGGGAAGGACACCAAATGAAAGATTGCACTGAGAGACAGGCTAATTTTT 
               
               
                           2040      2050      2060      2070      2080      2090 
               
               
                   
               
               
                          1910      1920      1930      1940      1950      1960 
               
               
                 BH 10  TAGGGAAGATCTGGCCTTCCTACAAGGGAAGGCCAGGGAATTTTCTTCAGAGCAGACCAG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAGGGAAGATCTGGCCTTCCTACAAGGGAAGGCCAGGGAATTTTCT-------------- 
               
               
                           2100      2110      2120      2130 
               
               
                   
               
               
                          1970      1980      1990      2000      2010      2020 
               
               
                 BH 10  AGCCAACAGCCCCACCATTTCTTCAGAGCAGACCAGAGCCAACAGCCCCACCAGAAGAGA 
               
               
                                              :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ----------------------TCAGAGCAGACCAGAGCCAACAGCCCCACCAGAAGAGA 
               
               
                                           2140      2150      2160      2170 
               
               
                   
               
               
                          2030      2040      2050      2060      2070      2080 
               
               
                 BH 10  GCTTCAGGTCTGGGGTAGAGACAACAACTCCCCCTCAGAAGCAGGAGCCGATAGACAAGG 
               
               
                        ::::::::: :::::  :::: :::::::::: ::::::::::::::::::::::::::: 
               
               
                 Licuw, GCTTCAGGTTTGGGGAGGAGAAAACAACTCCCTCTCAGAAGCAGGAGCCGATAGACAAGG 
               
               
                       2180      2190      2200      2210      2220      2230 
               
               
                   
               
               
                          2090      2100      2110      2120      2130      2140 
               
               
                 BH 10  AACTGTATCCTTTAACTTCCCTCAGATCACTCTTTGGCAACGACCCCTCGTCACAATAAA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: 
               
               
                 Licuw, AACTGTATCCTTTAACTTCCCTCAGATCACTCTTTGGCAACGACCCCTCGTCACAATTAA 
               
               
                       2240      2250      2260      2270      2280      2290 
               
               
                   
               
               
                           2150      2160      2170      2180      2190      2200 
               
               
                 BH 10  G-ATAGGGGGGCAACTAAAGGAAGCTCTATTAGATACAGGAGCAGATGATACAGTATTAG 
               
               
                        : :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GGATAGGGGGGCAACTAAAGGAAGCTCTATTAGATACAGGAGCAGATGATACAGTATTAG 
               
               
                       2300      2310      2320      2330      2340      2350 
               
               
                   
               
               
                           2210      2220      2230      2240      2250      2260 
               
               
                 BH 10  AAGAAATGAGTTTGCCAGGAAGATGGAAACCAAAAATGATAGGGGGAATTGGAGGTTTTA 
               
               
                        ::::::::: ::::::::::: :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AAGAAATGAATTTGCCAGGAAAATGGAAACCAAAAATGATAGGGGGAATTGGAGGTTTTA 
               
               
                       2360      2370      2380      2390      2400      2410 
               
               
                   
               
               
                           2270      2280      2290      2300      2310      2320 
               
               
                 BH 10  TCAAAGTAAGACAGTATGATCAGATACTCATAGAAATCTGTGGACATAAAGCTATAGGTA 
               
               
                        :::::::::::::::: ::::::::::   :::::::::::::::::::::::::::::: 
               
               
                 Licuw, TCAAAGTAAGACAGTACGATCAGATACCTGTAGAAATCTGTGGACATAAAGCTATAGGTA 
               
               
                       2420      2430      2440      2450      2460      2470 
               
               
                   
               
               
                           2330      2340      2350      2360      2370      2380 
               
               
                 BH 10  CAGTATTAGTAGGACCTACACCTGTCAACATAATTGGAAGAAATCTGTTGACTCAGATTG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CAGTATTAGTAGGACCTACACCTGTCAACATAATTGGAAGAAATCTGTTGACTCAGATTG 
               
               
                       2480      2490      2500      2510      2520      2530 
               
               
                   
               
               
                           2390      2400      2410      2420      2430      2440 
               
               
                 BH 10  GTTGCACTTTAAATTTTCCCATTAGCCCTATTGAGACTGTACCAGTAAAATTAAAGCCAG 
               
               
                        :::: ::::::::::: :::::::: :::::::: ::::::::::::::::::::::::: 
               
               
                 Licuw, GTTGTACTTTAAATTTCCCCATTAGTCCTATTGAAACTGTACCAGTAAAATTAAAGCCAG 
               
               
                       2540      2550      2560      2570      2580      2590 
               
               
                   
               
               
                           2450      2460      2470      2480      2490      2500 
               
               
                 BH 10  GAATGGATGGCCCAAAAGTTAAACAATGGCCATTGACAGAAGAAAAAATAAAAGCATTAG 
               
               
                        :::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GAATGGATGGCCCAAAAGTTAAGCAATGGCCATTGACAGAAGAAAAAATAAAAGCATTAG 
               
               
                       2600      2610      2620      2630      2640      2650 
               
               
                   
               
               
                           2510      2520      2530      2540      2550      2560 
               
               
                 BH 10  TAGAAATTTGTACAGAAATGGAAAAGGAAGGGAAAATTTCAAAAATTGGGCCTGAGAATC 
               
               
                        :::: :: ::::::::::::::::::::::::::::::::::::::::::::::: :::: 
               
               
                 Licuw, TAGAGATATGTACAGAAATGGAAAAGGAAGGGAAAATTTCAAAAATTGGGCCTGAAAATC 
               
               
                       2660      2670      2680      2690      2700      2710 
               
               
                   
               
               
                           2570      2580      2590      2600      2610      2620 
               
               
                 BH 10  CATACAATACTCCAGTATTTGCCATAAAGAAAAAAGACAGTACTAAATGGAGAAAATTAG 
               
               
                        :::::::::::::::::::::: ::::::::::::::::::::::::::::::::: ::: 
               
               
                 Licuw, CATACAATACTCCAGTATTTGCTATAAAGAAAAAAGACAGTACTAAATGGAGAAAACTAG 
               
               
                       2720      2730      2740      2750      2760      2770 
               
               
                   
               
               
                           2630      2640      2650      2660      2670      2680 
               
               
                 BH 10  TAGATTTCAGAGAACTTAATAAGAGAACTCAAGACTTCTGGGAAGTTCAATTAGGAATAC 
               
               
                        :::::::::::::::::::::: :::::::::::::::::::::::::: :::::::::: 
               
               
                 Licuw, TAGATTTCAGAGAACTTAATAAAAGAACTCAAGACTTCTGGGAAGTTCAGTTAGGAATAC 
               
               
                       2780      2790      2800      2810      2820      2830 
               
               
                   
               
               
                           2690      2700       2710      2720      2730      2740 
               
               
                 BH 10  CACATCCCGCAGGGTTAAAAAA-GAAAAAATCAGTAACAGTACTGGATGTGGGTGATGCA 
               
               
                        :::: ::::::::::::::::: ::::::::::::::::::: ::::::::::::::::: 
               
               
                 Licuw, CACA-CCCGCAGGGTTAAAAAAAGAAAAAATCAGTAACAGTATTGGATGTGGGTGATGCA 
               
               
                       2840       2850      2860      2870      2880      2890 
               
               
                   
               
               
                            2750      2760      2770      2780      2790      2800 
               
               
                 BH 10  TATTTTTCAGTTCCCTTAGATGAAGACTTCAGGAAGTATACTGCATTTACCATACCTAGT 
               
               
                        :: :::::::::::::::::: ::::::: :: ::::::::::::::::::::::::::: 
               
               
                 Licuw, TACTTTTCAGTTCCCTTAGATAAAGACTTTAGAAAGTATACTGCATTTACCATACCTAGT 
               
               
                        2900      2910      2920      2930      2940      2950 
               
               
                   
               
               
                            2810      2820      2830      2840       2850      2860 
               
               
                 BH 10  ATAAACAATGAGACACCAGGGATTAGATATCAGTACAATGTG-CTTCCACAGGGATGGAA 
               
               
                        :::::::::::::::::::::::::::::::::::::::::: :: :::::::::::::: 
               
               
                 Licuw, ATAAACAATGAGACACCAGGGATTAGATATCAGTACAATGTGGCTGCCACAGGGATGGAA 
               
               
                        2960      2970      2980      2990      3000      3010 
               
               
                   
               
               
                             2870      2880      2890      2900      2910      2920 
               
               
                 BH 10  AGGATCACCAGCAATATTCCAAAGTAGCATGACAAAAATCTTAGAGCCTTTTAAAAAACA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::: 
               
               
                 Licuw, AGGATCACCAGCAATATTCCAAAGTAGCATGACAAAAATCTTAGAGCCTTTTAGAAAACA 
               
               
                        3020      3030      3040      3050      3060      3070 
               
               
                   
               
               
                             2930      2940      2950      2960      2970      2980 
               
               
                 BH 10  AAATCCAGACATAGTTATCTATCAATACATGGATGATTTGTATGTAGGATCTGACTTAGA 
               
               
                         ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GAATCCAGACATAGTTATCTATCAATACATGGATGATTTGTATGTAGGATCTGACTTAGA 
               
               
                        3080      3090      3100      3110      3120      3130 
               
               
                   
               
               
                             2990      3000      3010      3020      3030      3040 
               
               
                 BH 10  AATAGGGCAGCATAGAACAAAAATAGAGGAGCTGAGACAACATCTGTTGAGGTGGGGACT 
               
               
                        :::::::::::::::::::::::::::::: :::::::: :::::::::::::::::: : 
               
               
                 Licuw, AATAGGGCAGCATAGAACAAAAATAGAGGAACTGAGACAGCATCTGTTGAGGTGGGGATT 
               
               
                        3140      3150      3160      3170      3180      3190 
               
               
                   
               
               
                             3050      3060      3070      3080      3090      3100 
               
               
                 BH 10  TACCACACCAGACAAAAAACATCAGAAAGAACCTCCATTCCTTTGGATGGGTTATGAACT 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TACCACACCAGACAAAAAACATCAGAAAGAACCTCCATTCCTTTGGATGGGTTATGAACT 
               
               
                        3200      3210      3220      3230      3240      3250 
               
               
                   
               
               
                             3110      3120      3130      3140      3150      3160 
               
               
                 BH 10  CCATCCTGATAAATGGACAGTACAGCCTATAGTGCTGCCAGAAAAAGACAGCTGGACTGT 
               
               
                        ::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::: 
               
               
                 Licuw, CCATCCTGATAAATGGACAGTACAGCCTATAATGCTGCCAGAAAAAGACAGCTGGACTGT 
               
               
                        3260      3270      3280      3290      3300      3310 
               
               
                   
               
               
                             3170      3180      3190      3200      3210      3220 
               
               
                 BH 10  CAATGACATACAGAAGTTAGTGGGGAAATTGAATTGGGCAAGTCAGATTTACCCAGGGAT 
               
               
                        :::::::::::::::::::::::: ::::::::::::::::::::::::::  ::::::: 
               
               
                 Licuw, CAATGACATACAGAAGTTAGTGGGAAAATTGAATTGGGCAAGTCAGATTTATGCAGGGAT 
               
               
                        3320      3330      3340      3350      3360      3370 
               
               
                   
               
               
                             3230      3240      3250      3260      3270      3280 
               
               
                 BH 10  TAAAGTAAGGCAATTATGTAAACTCCTTAGAGGAACCAAAGCACTAACAGAAGTAATACC 
               
               
                        :::::::: ::: ::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TAAAGTAAAGCAGTTATGTAAACTCCTTAGAGGAACCAAAGCACTAACAGAAGTAATACC 
               
               
                        3380      3390      3400      3410      3420      3430 
               
               
                   
               
               
                             3290      3300      3310      3320      3330      3340 
               
               
                 BH 10  ACTAACAGAAGAAGCAGAGCTAGAACTGGCAGAAAACAGAGAGATTCTAAAAGAACCAGT 
               
               
                        ::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::: 
               
               
                 Licuw, ACTAACAGAAGAAGCAGAGCTAGAACTGGCAGAAAACAGGGAGATTCTAAAAGAACCAGT 
               
               
                        3440      3450      3460      3470      3480      3490 
               
               
                   
               
               
                             3350      3360      3370      3380      3390      3400 
               
               
                 BH 10  ACATGGAGTGTATTATGACCCATCAAAAGACTTAATAGCAGAAATACAGAAGCAGGGGCA 
               
               
                        ::::: ::: :::::::::::::::::::::::: ::::::::::::::::::::::::: 
               
               
                 Licuw, ACATGAAGTATATTATGACCCATCAAAAGACTTAGTAGCAGAAATACAGAAGCAGGGGCA 
               
               
                        3500      3510      3520      3530      3540      3550 
               
               
                   
               
               
                             3410      3420      3430      3440      3450      3460 
               
               
                 BH 10  AGGCCAATGGACATATCAAATTTATCAAGAGCCATTTAAAAATCTGAAAACAGGAAAATA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: 
               
               
                 Licuw, AGGCCAATGGACATATCAAATTTATCAAGAGCCATTTAAAAATCTGAAAACAGGAAAGTA 
               
               
                        3560      3570      3580      3590      3600      3610 
               
               
                   
               
               
                             3470      3480      3490      3500      3510      3520 
               
               
                 BH 10  TGCAAGAATGAGGGGTGCCCACACTAATGATGTAAAACAATTAACAGAGGCAGTGCAAAA 
               
               
                        :::::: :::::::::::::::::::::::::::::::: :::::::::::::::::::: 
               
               
                 Licuw, TGCAAGGATGAGGGGTGCCCACACTAATGATGTAAAACAGTTAACAGAGGCAGTGCAAAA 
               
               
                        3620      3630      3640      3650      3660      3670 
               
               
                   
               
               
                             3530      3540      3550      3560      3570      3580 
               
               
                 BH 10  AATAACCACAGAAAGCATAGTAATATGGGGAAAGACTCCTAAATTTAAACTACCCATACA 
               
               
                        : :: :::::::::::::::::::::::::::::: :::::::::::::::::::::::: 
               
               
                 Licuw, AGTATCCACAGAAAGCATAGTAATATGGGGAAAGATTCCTAAATTTAAACTACCCATACA 
               
               
                        3680      3690      3700      3710      3720      3730 
               
               
                   
               
               
                             3590      3600      3610      3620      3630      3640 
               
               
                 BH 10  AAAGGAAACATGGGAAACATGGTGGACAGAGTATTGGCAAGCCACCTGGATTCCTGAGTG 
               
               
                        :::::::::::::::: :::::::::  :::::::::::::: ::::::::::::::::: 
               
               
                 Licuw, AAAGGAAACATGGGAAGCATGGTGGATGGAGTATTGGCAAGCTACCTGGATTCCTGAGTG 
               
               
                        3740      3750      3760      3770      3780      3790 
               
               
                   
               
               
                             3650      3660      3670      3680      3690      3700 
               
               
                 BH 10  GGAGTTTGTTAATACCCCTCCTTTAGTGAAATTATGGTACCAGTTAGAGAAAGAACCCAT 
               
               
                        ::::::::: ::::::::::: :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GGAGTTTGTCAATACCCCTCCCTTAGTGAAATTATGGTACCAGTTAGAGAAAGAACCCAT 
               
               
                        3800      3810      3820      3830      3840      3850 
               
               
                   
               
               
                             3710      3720      3730      3740      3750      3760 
               
               
                 BH 10  AGTAGGAGCAGAAACCTTCTATGTAGATGGGGCAGCTAACAGGGAGACTAAATTAGGAAA 
               
               
                        ::::::::::::::: ::::::::::::::::::::::: :::::::::::::::::::: 
               
               
                 Licuw, AGTAGGAGCAGAAACTTTCTATGTAGATGGGGCAGCTAATAGGGAGACTAAATTAGGAAA 
               
               
                        3860      3870      3880      3890      3900      3910 
               
               
                   
               
               
                             3770      3780      3790      3800      3810      3820 
               
               
                 BH 10  AGCAGGATATGTTACTAACAAAGGAAGACAAAAGGTTGTCCCCCTAACTAACACAACAAA 
               
               
                        :::::::::::::::: ::: :::::::::::: :::::: :: :: :: :::::::::: 
               
               
                 Licuw, AGCAGGATATGTTACTGACAGAGGAAGACAAAAAGTTGTCTCCATAGCTGACACAACAAA 
               
               
                        3920      3930      3940      3950      3960      3970 
               
               
                   
               
               
                             3830      3840      3850      3860      3870      3880 
               
               
                 BH 10  TCAGAAAACTGAGTTACAAGCAATTTATCTAGCTTTGCAGGATTCAGGATTAGAAGTAAA 
               
               
                        :::::: ::::: :::::::::::: ::::::::::::::::::: :::::::::::::: 
               
               
                 Licuw, TCAGAAGACTGAATTACAAGCAATTCATCTAGCTTTGCAGGATTCGGGATTAGAAGTAAA 
               
               
                        3980      3990      4000      4010      4020      4030 
               
               
                   
               
               
                             3890      3900      3910      3920      3930      3940 
               
               
                 BH 10  CATAGTAACAGACTCACAATATGCATTAGGAATCATTCAAGCACAACCAGATAAAAGTGA 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::: 
               
               
                 Licuw, CATAGTAACAGACTCACAATATGCATTAGGAATCATTCAAGCACAACCAGATAAGAGTGA 
               
               
                        4040      4050      4060      4070      4080      4090 
               
               
                   
               
               
                             3950      3960      3970      3980      3990      4000 
               
               
                 BH 10  ATCAGAGTTAGTCAATCAAATAATAGAGCAGTTAATAAAAAAGGAAAAGGTCTATCTGGC 
               
               
                        :::::::::::::: ::::::::::::::::::::::::::::::::::::::: ::::: 
               
               
                 Licuw, ATCAGAGTTAGTCAGTCAAATAATAGAGCAGTTAATAAAAAAGGAAAAGGTCTACCTGGC 
               
               
                        4100      4110      4120      4130      4140      4150 
               
               
                   
               
               
                             4010      4020      4030      4040      4050      4060 
               
               
                 BH 10  ATGGGTACCAGCACACAAAGGAATTGGAGGAAATGAACAAGTAGATAAATTAGTCAGTGC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ATGGGTACCAGCACACAAAGGAATTGGAGGAAATGAACAAGTAGATAAATTAGTCAGTGC 
               
               
                        4160      4170      4180      4190      4200      4210 
               
               
                   
               
               
                             4070      4080      4090      4100      4110      4120 
               
               
                 BH 10  TGGAATCAGGAAAATACTATTTTTAGATGGAATAGATAAGGCCCAAGATGAACATGAGAA 
               
               
                        ::::::::::::: ::::::::::  :::::::::::::::::::::: ::::::::::: 
               
               
                 Licuw, TGGAATCAGGAAAGTACTATTTTTGAATGGAATAGATAAGGCCCAAGAAGAACATGAGAA 
               
               
                        4220      4230      4240      4250      4260      4270 
               
               
                   
               
               
                             4130      4140      4150      4160      4170      4180 
               
               
                 BH 10  ATATCACAGTAATTGGAGAGCAATGGCTAGTGATTTTAACCTGCCACCTGTAGTAGCAAA 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ATATCACAGTAATTGGAGAGCAATGGCTAGTGATTTTAACCTGCCACCTGTAGTAGCAAA 
               
               
                        4280      4290      4300      4310      4320      4330 
               
               
                   
               
               
                             4190      4200      4210      4220      4230      4240 
               
               
                 BH 10  AGAAATAGTAGCCAGCTGTGATAAATGTCAGCTAAAAGGAGAAGCCATGCATGGACAAGT 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AGAAATAGTAGCCAGCTGTGATAAATGTCAGCTAAAAGGAGAAGCCATGCATGGACAAGT 
               
               
                        4340      4350      4360      4370      4380      4390 
               
               
                   
               
               
                             4250      4260      4270      4280      4290      4300 
               
               
                 BH 10  AGACTGTAGTCCAGGAATATGGCAACTAGATTGTACACATTTAGAAGGAAAAGTTATCCT 
               
               
                        :::::::::::::::::::::::::::::::::::::::: ::::::::::: ::::::: 
               
               
                 Licuw, AGACTGTAGTCCAGGAATATGGCAACTAGATTGTACACATCTAGAAGGAAAAATTATCCT 
               
               
                        4400      4410      4420      4430      4440      4450 
               
               
                   
               
               
                             4310      4320      4330      4340      4350      4360 
               
               
                 BH 10  GGTAGCAGTTCATGTAGCCAGTGGATATATAGAAGCAGAAGTTATTCCAGCAGAAACAGG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::: 
               
               
                 Licuw, GGTAGCAGTTCATGTAGCCAGTGGATATATAGAAGCAGAAGTTATTCCAGCAGAGACAGG 
               
               
                        4460      4470      4480      4490      4500      4510 
               
               
                   
               
               
                             4370      4380      4390      4400      4410      4420 
               
               
                 BH 10  GCAGGAAACAGCATATTTTCTTTTAAAATTAGCAGGAAGATGGCCAGTAAAAACAATACA 
               
               
                        ::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GCAGGAAACAGCATATTTTCTCTTAAAATTAGCAGGAAGATGGCCAGTAAAAACAATACA 
               
               
                        4520      4530      4540      4550      4560      4570 
               
               
                   
               
               
                             4430      4440      4450      4460      4470      4480 
               
               
                 BH 10  TACAGACAATGGCAGCAATTTCACCAGTGCTACGGTTAAGGCCGCCTGTTGGTGGGCGGG 
               
               
                        :::::::::::::::::::::::::::: :::::::::::::::::::::::::::: :: 
               
               
                 Licuw, TACAGACAATGGCAGCAATTTCACCAGTACTACGGTTAAGGCCGCCTGTTGGTGGGCAGG 
               
               
                        4580      4590      4600      4610      4620      4630 
               
               
                   
               
               
                             4490      4500      4510      4520      4530      4540 
               
               
                 BH 10  AATCAAGCAGGAATTTGGAATTCCCTACAATCCCCAAAGTCAAGGAGTAGTAGAATCTAT 
               
               
                         ::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GATCAAGCAGGAATTTGGCATTCCCTACAATCCCCAAAGTCAAGGAGTAGTAGAATCTAT 
               
               
                        4640      4650      4660      4670      4680      4690 
               
               
                   
               
               
                             4550      4560      4570      4580      4590      4600 
               
               
                 BH 10  GAATAAAGAATTAAAGAAAATTATAGGACAGGTAAGAGATCAGGCTGAACATCTTAAGAC 
               
               
                        :::::: :::::::::::::::::::::::::::::::::::::::::::: :::::::: 
               
               
                 Licuw, GAATAATGAATTAAAGAAAATTATAGGACAGGTAAGAGATCAGGCTGAACACCTTAAGAC 
               
               
                        4700      4710      4720      4730      4740      4750 
               
               
                   
               
               
                             4610      4620      4630      4640      4650      4660 
               
               
                 BH 10  AGCAGTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTGGGGGGTA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: 
               
               
                 Licuw, AGCAGTACAAATGGCAGTATTCATCCACAATTTTAAAAGAAAAGGGGGGATTGGGGGATA 
               
               
                        4760      4770      4780      4790      4800      4810 
               
               
                   
               
               
                             4670      4680      4690      4700      4710      4720 
               
               
                 BH 10  CAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAATTACA 
               
               
                        ::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::: 
               
               
                 Licuw, CAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATACAAACTAAAGAACTACA 
               
               
                        4820      4830      4840      4850      4860      4870 
               
               
                   
               
               
                             4730      4740      4750      4760      4770      4780 
               
               
                 BH 10  AAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTACAGGGACAGCAGAAATCCACT 
               
               
                        ::: ::::::::::::::::::::::::::::::::::::::::::: :: : :::: :: 
               
               
                 Licuw, AAAGCAAATTACAAAAATTCAAAATTTTCGGGTTTATTACAGGGACAACAAAGATCCCCT 
               
               
                        4880      4890      4900      4910      4920      4930 
               
               
                   
               
               
                             4790      4800      4810      4820      4830      4840 
               
               
                 BH 10  TTGGAAAGGACCAGCAAAGCTCCTCTGGAAAGGTGAAGGGGCAGTAGTAATACAAGATAA 
               
               
                        ::::::::::::::::::::: ::::: :::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TTGGAAAGGACCAGCAAAGCTTCTCTGCAAAGGTGAAGGGGCAGTAGTAATACAAGATAA 
               
               
                        4940      4950      4960      4970      4980      4990 
               
               
                   
               
               
                             4850      4860      4870      4880      4890      4900 
               
               
                 BH 10  TAGTGACATAAAAGTAGTGCCAAGAAGAAAAGCAAAGATCATTAGGGATTATGGAAAACA 
               
               
                        :::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::: 
               
               
                 Licuw, TAGTGACATAAAAGTAGTGCCAAGAAGAAAAGCAAAAATCATTAGGGATTATGGAAAACA 
               
               
                        5000      5010      5020      5030      5040      5050 
               
               
                   
               
               
                             4910      4920      4930      4940      4950      4960 
               
               
                 BH 10  GATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAGGATTAGAACATGGAAAAGTT 
               
               
                        :::::::::::::::::::::   :::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GATGGCAGGTGATGATTGTGT---AAGTAGACAGGATGAGGATTAGAACATGGAAAAGTT 
               
               
                        5060      5070         5080      5090      5100      5110 
               
               
                   
               
               
                             4970      4980      4990      5000      5010      5020 
               
               
                 BH 10  TAGTAAAACACCATATGTATGTTTCAGGGAAAGCTAGGGGATGGTTTTATAGACATCACT 
               
               
                        :::::::::::::::::::: :::::  ::::::::  :::::::::: ::: ::::::: 
               
               
                 Licuw, TAGTAAAACACCATATGTATATTTCAAAGAAAGCTAAAGGATGGTTTT-TAG-CATCACT 
               
               
                           5120      5130      5140      5150      5160        5170 
               
               
                   
               
               
                             5030      5040      5050      5060      5070      5080 
               
               
                 BH 10  ATGAAAGCCCTCATCCAAGAATAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGAT 
               
               
                         ::::::  ::::::::::: :::::::::::::::::::::: ::::::::::::: :: 
               
               
                 Licuw, -TGAAAGTACTCATCCAAGAGTAAGTTCAGAAGTACACATCCCCCTAGGGGATGCTAAAT 
               
               
                              5180      5190      5200      5210      5220      5230 
               
               
                   
               
               
                             5090      5100      5110       5120      5130      5140 
               
               
                 BH 10  TGGTAATAACAACATATTGGGGTCTGCATACA-GGAGAAAGAGACTGGCATTTGGGTCAG 
               
               
                        :::::::::::::::::::::::::::::::: ::::::::::: ::::::::::: ::: 
               
               
                 Licuw, TGGTAATAACAACATATTGGGGTCTGCATACAAGGAGAAAGAGAATGGCATTTGGG-CAG 
               
               
                              5240      5250      5260      5270      5280 
               
               
                   
               
               
                              5150      5160      5170      5180      5190      5200 
               
               
                 BH 10  GGAGTCTCCATAGAATGGAGGAAAAAGAGATATAGCACACAAGTAGACCCTGAACTAGCA 
               
               
                        :: ::: :::::::::::::  :::::: :: ::::::::::::::::::::  :::::: 
               
               
                 Licuw, GG-GTCGCCATAGAATGGAG--AAAAGA-AT-TAGCACACAAGTAGACCCTGGCCTAGCA 
               
               
                     5290       5300        5310        5320      5330      5340 
               
               
                   
               
               
                              5210      5220      5230      5240      5250      5260 
               
               
                 BH 10  GACCAACTAATTCATCTGTATTACTTTGACTGTTTTTCAGACTCTGCTATAAGAAAGGCC 
               
               
                        :::::::::::::::::: :::: ::::: ::::::::::: :::::::::: ::: ::: 
               
               
                 Licuw, GACCAACTAATTCATCTGCATTATTTTGATTGTTTTTCAGAATCTGCTATAAAAAATGCC 
               
               
                          5350      5360      5370      5380      5390      5400 
               
               
                   
               
               
                              5270      5280      5290      5300      5310      5320 
               
               
                 BH 10  TTATTAGGACACATAGTTAGCCCTAGGTGTGAATATCAAGCAGGACATAACAAGGTAGGA 
               
               
                         :::::::: : :::::: ::::: ::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ATATTAGGATATAGAGTTAGTCCTAGCTGTGAATATCAAGCAGGACATAACAAGGTAGGA 
               
               
                          5410      5420      5430      5440      5450      5460 
               
               
                   
               
               
                              5330      5340      5350      5360      5370      5380 
               
               
                 BH 10  TCTCTACAATACTTGGCACTAGCAGCATTAATAACACCAAAAAAGATAAAGCCACCTTTG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::: 
               
               
                 Licuw, TCTCTACAATACTTGGCACTAGCAGCATTAATAACACCAAAAAAGACAAAGCCACCTTTG 
               
               
                          5470      5480      5490      5500      5510      5520 
               
               
                   
               
               
                              5390      5400      5410      5420      5430      5440 
               
               
                 BH 10  CCTAGTGTTACGAAACTGACAGAGGATAGATGGAACAAGCCCCAGAAGACCAAGGGCCAC 
               
               
                        :::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CCTAGTGTTAAGAAACTGACAGAGGATAGATGGAACAAGCCCCAGAAGACCAAGGGCCAC 
               
               
                          5530      5540      5550      5560      5570      5580 
               
               
                   
               
               
                              5450      5460      5470      5480      5490      5500 
               
               
                 BH 10  AGAGGGAGCCACACAATGAATGGACACTAGAGCTTTTAGAGGAGCTTAAGAATGAAGCTG 
               
               
                        ::::::::::: :::::::::::::::::::::::::::::::::::::::  ::::::: 
               
               
                 Licuw, AGAGGGAGCCATACAATGAATGGACACTAGAGCTTTTAGAGGAGCTTAAGAGAGAAGCTG 
               
               
                          5590      5600      5610      5620      5630      5640 
               
               
                   
               
               
                              5510      5520      5530      5540      5550      5560 
               
               
                 BH 10  TTAGACATTTTCCTAGGATTTGGCTCCATGGCTTAGGGCAACATATCTATGAAACTTATG 
               
               
                        :::::::::::::::::   ::::::::: ::::::: ::: :::::::::::::::::: 
               
               
                 Licuw, TTAGACATTTTCCTAGGCCATGGCTCCATAGCTTAGGACAATATATCTATGAAACTTATG 
               
               
                          5650      5660      5670      5680      5690      5700 
               
               
                   
               
               
                              5570      5580      5590      5600      5610      5620 
               
               
                 BH 10  GGGATACTTGGGCAGGAGTGGAAGCCATAATAAGAATTCTGCAACAACTGCTGTTTATCC 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: : 
               
               
                 Licuw, GGGATACTTGGGCAGGAGTGGAAGCCATAATAAGAATTCTGCAACAACTGCTGTTTATTC 
               
               
                          5710      5720      5730      5740      5750      5760 
               
               
                   
               
               
                              5630      5640      5650      5660      5670      5680 
               
               
                 BH 10  ATTTTCAGAATTGGGTGTCGACATAGCAGAATAGGCGTTACTCGACAGAGGAGAGCAAGA 
               
               
                        :::: :::::::::::::: :::::::::::::::: ::: :: :::::::::::::::: 
               
               
                 Licuw, ATTT-CAGAATTGGGTGTCAACATAGCAGAATAGGCATTATTCAACAGAGGAGAGCAAGA 
               
               
                           5770      5780      5790      5800      5810      5820 
               
               
                   
               
               
                                 5690      5700      5710      5720      5730 
               
               
                 BH 10  A---ATGGAGCCAGTAGATCCTAGACTAGAGCCCTGGAAGCATCCAGGAAGTCAGCCTAA 
               
               
                        :   :::::::::::::::::::  :::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AGAAATGGAGCCAGTAGATCCTAATCTAGAGCCCTGGAAGCATCCAGGAAGTCAGCCTAG 
               
               
                           5830      5840      5850      5860      5870      5880 
               
               
                   
               
               
                       5740      5750      5760      5770      5780      5790 
               
               
                 BH 10  AACTGCTTGTACCAATTGCTATTGTAAAAAGTGTTGCTTTCATTGCCAAGTTTGTTTCAT 
               
               
                         :::::::::: :::::::::::::::::::::::::::::::::: : :  ::::::: 
               
               
                 Licuw, GACTGCTTGTAACAATTGCTATTGTAAAAAGTGTTGCTTTCATTGCTACGCGTGTTTCAC 
               
               
                           5890      5900      5910      5920      5930      5940 
               
               
                   
               
               
                       5800      5810      5820      5830      5840      5850 
               
               
                 BH 10  AACAAAAGCCTTAGGCATCTCCTATGGCAGGAAGAAGCGGAGACAGCGACGAAGACCTCC 
               
               
                        :: ::::: :::::::::::::::::::::::::::::::::::::::::::::: :::: 
               
               
                 Licuw, AAGAAAAGGCTTAGGCATCTCCTATGGCAGGAAGAAGCGGAGACAGCGACGAAGAGCTCC 
               
               
                           5950      5960      5970      5980      5990      6000 
               
               
                   
               
               
                       5860      5870      5880      5890      5900      5910 
               
               
                 BH 10  TCAAGGCAGTCAGACTCATCAAGTTTCTCTATCAAAGCAGTAAGTAGTACATGTAATGCA 
               
               
                        ::: : ::::::::::::::::: ::::::::::::::::::::::::: :::::::::: 
               
               
                 Licuw, TCAGGACAGTCAGACTCATCAAGCTTCTCTATCAAAGCAGTAAGTAGTAAATGTAATGCA 
               
               
                           6010      6020      6030      6040      6050      6060 
               
               
                   
               
               
                       5920      5930         5940      5950      5960      5970 
               
               
                 BH 10  ACCTATACAAATA---GCAATAGTAGCATTAGTAGTAGCAATAATAATAGCAATAGTTGT 
               
               
                        : :: ::::::::   ::::::::: :::::::::::: :  :::::::::::::::::: 
               
               
                 Licuw, ATCTTTACAAATATTAGCAATAGTATCATTAGTAGTAGTAGCAATAATAGCAATAGTTGT 
               
               
                           6070      6080      6090      6100      6110      6120 
               
               
                   
               
               
                          5980      5990      6000      6010      6020      6030 
               
               
                 BH 10  GTGGTCCATAGTAATCATAGAATATAGGAAAATATTAAGACAAAGAAAAATAGACAGGTT 
               
               
                        :::: :::::::: ::::::::::::::::::::::::::::::::::: ::::::: :: 
               
               
                 Licuw, GTGGACCATAGTACTCATAGAATATAGGAAAATATTAAGACAAAGAAAA-TAGACAGATT 
               
               
                           6130      6140      6150      6160      6170       6180 
               
               
                   
               
               
                          6040      6050      6060      6070      6080 
               
               
                 BH 10  AATTGATAGACTAATAGAAAGAGCAGAAGACAGTGGCAATGAGAGTGAAGGAGA------ 
               
               
                        :::::::::: ::: ::::: ::::::::::::::::::::: :::::::: :: 
               
               
                 Licuw, AATTGATAGAATAAGAGAAAAAGCAGAAGACAGTGGCAATGAAAGTGAAGGGGACCAGGA 
               
               
                            6190      6200      6210      6220      6230      6240 
               
               
                   
               
               
                         6090      6100      6110      6120      6130      6140 
               
               
                 BH 10  ---AATATCAGCACTTGTGGAGATGGGGGTGGAGATGGGGCACCATGCTCCTTGGGATGT 
               
               
                           : :::::::::::::::::::::::            :::: ::::::::::::::: 
               
               
                 Licuw, GGAATTATCAGCACTTGTGGAGATGGGG------------CACCTTGCTCCTTGGGATGT 
               
               
                            6250      6260      6270                  6280      6290 
               
               
                   
               
               
                         6150      6160      6170      6180      6190      6200 
               
               
                 BH 10  TGATGATCTGTAGTGCTACAGAAAAATTGTGGGTCACAGTCTATTATGGGGTACCTGTGT 
               
               
                        :::::::::::::::::::::::::::::::::::::::: :::::::: :::::::::: 
               
               
                 Licuw, TGATGATCTGTAGTGCTACAGAAAAATTGTGGGTCACAGTTTATTATGGAGTACCTGTGT 
               
               
                              6300      6310      6320      6330      6340      6350 
               
               
                   
               
               
                         6210      6220      6230      6240      6250      6260 
               
               
                 BH 10  GGAAGGAAGCAACCACCACTCTATTTTGTGCATCAGATGCTAAAGCATATGATACAGAGG 
               
               
                        :::: :::::::: :::::::::::::::::::::::::::: ::::::::::::::::: 
               
               
                 Licuw, GGAAAGAAGCAACTACCACTCTATTTTGTGCATCAGATGCTAGAGCATATGATACAGAGG 
               
               
                              6360      6370      6380      6390      6400      6410 
               
               
                   
               
               
                         6270      6280      6290      6300      6310      6320 
               
               
                 BH 10  TACATAATGTTTGGGCCACACATGCCTGTGTACCCACAGACCCCAACCCACAAGAAGTAG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TACATAATGTTTGGGCCACACATGCCTGTGTACCCACAGACCCCAACCCACAAGAAGTAG 
               
               
                              6420      6430      6440      6450      6460      6470 
               
               
                   
               
               
                         6330      6340      6350      6360      6370      6380 
               
               
                 BH 10  TATTGGTAAATGTGACAGAAAATTTTAACATGTGGAAAAATGACATGGTAGAACAGATGC 
               
               
                        :::::: :::::::::::::::::::::::::::::::::: :::::::::::::::::: 
               
               
                 Licuw, TATTGGGAAATGTGACAGAAAATTTTAACATGTGGAAAAATAACATGGTAGAACAGATGC 
               
               
                              6480      6490      6500      6510      6520      6530 
               
               
                   
               
               
                         6390      6400      6410      6420      6430      6440 
               
               
                 BH 10  ATGAGGATATAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAATTAACCCCAC 
               
               
                        : :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, AGGAGGATATAATCAGTTTATGGGATCAAAGCCTAAAGCCATGTGTAAAATTAACCCCAC 
               
               
                              6540      6550      6560      6570      6580      6590 
               
               
                   
               
               
                         6450      6460      6470      6480      6490      6500 
               
               
                 BH 10  TCTGTGTTAGTTTAAAGTGCACTGATTTGAAGAATGATACTAATACCAATAGTAGTAGCG 
               
               
                        ::::::::: :::::: ::::::::::::  ::: : :::::::::::::::::::: 
               
               
                 Licuw, TCTGTGTTACTTTAAATTGCACTGATTTGGGGAAGGCTACTAATACCAATAGTAGTAATT 
               
               
                              6600      6610      6620      6630      6640      6650 
               
               
                   
               
               
                         6510       6520      6530      6540      6550      6560 
               
               
                 BH 10  GGAGA-ATGATAATGGAGAAAGGAGAGATAAAAAACTGCTCTTTCAATATCAGCACAAGC 
               
               
                        ::: : : :: :::    :::::::: ::::::::::::::::::::::::: ::::::: 
               
               
                 Licuw, GGAAAGAAGA-AATA---AAAGGAGAAATAAAAAACTGCTCTTTCAATATCACCACAAGC 
               
               
                              6660          6670      6680      6690      6700 
               
               
                   
               
               
                          6570      6580      6590      6600      6610      6620 
               
               
                 BH 10  ATAAGAGGTAAGGTGCAGAAAGAATATGCATTTTTTTATAAACTTGATATAATACCAATA 
               
               
                        ::::::: :::: : ::::::::: ::::: :::::  ::: :::::: :: :::::::: 
               
               
                 Licuw, ATAAGAGATAAGATTCAGAAAGAAAATGCACTTTTTCGTAACCTTGATGTAGTACCAATA 
               
               
                        6710      6720      6730      6740      6750      6760 
               
               
                   
               
               
                          6630            6640               6650      6660 
               
               
                 BH 10  GATAATGATA------CTACCAGCTATAC---------GTTGACAAGTTGTAACACCTCA 
               
               
                        ::::::: ::      :::::: ::::::         ::::: :  ::::::::  ::: 
               
               
                 Licuw, GATAATGCTAGTACTACTACCAACTATACCAACTATAGGTTGATACATTGTAACAGATCA 
               
               
                        6770      6780      6790      6800      6810      6820 
               
               
                   
               
               
                     6670      6680      6690      6700      6710      6720 
               
               
                 BH 10  GTCATTACACAGGCCTGTCCAAAGGTATCCTTTGAGCCAATTCCCATACATTATTGTGCC 
               
               
                        ::::::::::::::::::::::::::::: ::::::::::::::::::::::::::: :: 
               
               
                 Licuw, GTCATTACACAGGCCTGTCCAAAGGTATCATTTGAGCCAATTCCCATACATTATTGTACC 
               
               
                        6830      6840      6850      6860      6870      6880 
               
               
                   
               
               
                     6730      6740      6750      6760      6770      6780 
               
               
                 BH 10  CCGGCTGGTTTTGCGATTCTAAAATGTAATAATAAGACGTTCAATGGAACAGGACCATGT 
               
               
                        ::::::::::::::::::::::: ::::::::::: ::::::::::::: :::::::::: 
               
               
                 Licuw, CCGGCTGGTTTTGCGATTCTAAAGTGTAATAATAAAACGTTCAATGGAAAAGGACCATGT 
               
               
                        6890      6900      6910      6920      6930      6940 
               
               
                   
               
               
                     6790      6800      6810      6820      6830      6840 
               
               
                 BH 10  ACAAATGTCAGCACAGTACAATGTACACATGGAATTAGGCCAGTAGTATCAACTCAACTG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::: :::: :::::::::::: 
               
               
                 Licuw, ACAAATGTCAGCACAGTACAATGTACACATGGAATTAGGCCAATAGTGTCAACTCAACTG 
               
               
                        6950      6960      6970      6980      6990      7000 
               
               
                   
               
               
                      6850      6860      6870      6880      6890      6900 
               
               
                 BH 10  -CTGTTAAATGGCAGTCTAGCAGAAGAAGAGGTAGTAATTAGATCTGCCAATTTCACAGA 
               
               
                         :::::::::::::::::::::::::::::::::::::::::::::: :::::::::  : 
               
               
                 Licuw, TCTGTTAAATGGCAGTCTAGCAGAAGAAGAGGTAGTAATTAGATCTGACAATTTCACGAA 
               
               
                        7010      7020      7030      7040      7050      7060 
               
               
                   
               
               
                      6910      6920      6930      6940      6950      6960 
               
               
                 BH 10  CAATGCTAAAACCATAATAGTACAGCTGAACCAATCTGTAGAAATTAATTGTACAAGACC 
               
               
                        ::::::::::::::::::::::::::::::  ::::::::: :::::: ::::::::::: 
               
               
                 Licuw, CAATGCTAAAACCATAATAGTACAGCTGAATGAATCTGTAGCAATTAACTGTACAAGACC 
               
               
                        7070      7080      7090      7100      7110      7120 
               
               
                   
               
               
                      6970      6980      6990      7000      7010      7020 
               
               
                 BH 10  CAACAACAATACAAGAAAAAGTATCCGTATCCAGAGAGGACCAGGGAGAGCATTTGTTAC 
               
               
                        :::::::::::::::::::::::::  :::      :::::::::::::::::::  ::: 
               
               
                 Licuw, CAACAACAATACAAGAAAAAGTATCTATAT------AGGACCAGGGAGAGCATTTCATAC 
               
               
                        7130      7140      7150            7160      7170      7180 
               
               
                   
               
               
                      7030      7040         7050      7060      7070      7080 
               
               
                 BH 10  AATAGGAAAAATA---GGAAATATGAGACAAGCACATTGTAACATTAGTAGAGCAAAATG 
               
               
                        :: ::::: ::::   ::: :::: ::: :::::::::::::::::::::::::: :::: 
               
               
                 Licuw, AACAGGAAGAATAATAGGAGATATAAGAAAAGCACATTGTAACATTAGTAGAGCACAATG 
               
               
                              7190      7200      7210      7220      7230      7240 
               
               
                   
               
               
                         7090      7100      7110      7120      7130      7140 
               
               
                 BH 10  GAATAACACTTTAAAACAGATAGATAGCAAATTAAGAGAACAATTTGGAAATAATAAAAC 
               
               
                        ::::::::::::: ::::::::: ::  :::::::::::::: ::::: ::::::::::: 
               
               
                 Licuw, GAATAACACTTTAGAACAGATAGTTAAAAAATTAAGAGAACAGTTTGGGAATAATAAAAC 
               
               
                              7250      7260      7270      7280      7290      7300 
               
               
                   
               
               
                         7150      7160      7170      7180      7190      7200 
               
               
                 BH 10  AATAATCTTTAAGCAGTCCTCAGGAGGGGACCCAGAAATTGTAACGCACAGTTTTAATTG 
               
               
                        :::: ::::::: :: :::::::::::::::::::::::::::: ::::::::::::::: 
               
               
                 Licuw, AATAGTCTTTAATCAATCCTCAGGAGGGGACCCAGAAATTGTAATGCACAGTTTTAATTG 
               
               
                              7310      7320      7330      7340      7350      7360 
               
               
                   
               
               
                         7210      7220      7230      7240      7250        7260 
               
               
                 BH 10  TGGAGGGGAATTTTTCTACTGTAATTCAACACAACTGTTTAATAGTACTTGGT--TTAAT 
               
               
                        : ::::::::::::::::::::::: :::::::::::::::::: ::: :::   :::: 
               
               
                 Licuw, TAGAGGGGAATTTTTCTACTGTAATACAACACAACTGTTTAATAATACATGGAGGTTAA- 
               
               
                              7370      7380      7390      7400      7410 
               
               
                   
               
               
                           7270      7280      7290      7300      7310      7320 
               
               
                 BH 10  AGTACTTGGAGTACTAAAGGGTCAAATAACACTGAAGGAAGTGACACAATCACCCTCCCA 
               
               
                        :  ::       ::: ::::          ::: :::::: :::::::::::  :::::: 
               
               
                 Licuw, ATCAC-------ACTGAAGGA---------ACTAAAGGAAATGACACAATCATACTCCCA 
               
               
                     7420             7430               7440      7450      7460 
               
               
                   
               
               
                           7330      7340      7350      7360      7370      7380 
               
               
                 BH 10  TGCAGAATAAAACAAATTATAAACATGTGGCAGGAAGTAGGAAAAGCAATGTATGCCCCT 
               
               
                        :: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TGTAGAATAAAACAAATTATAAACATGTGGCAGGAAGTAGGAAAAGCAATGTATGCCCCT 
               
               
                           7470      7480      7490      7500      7510      7520 
               
               
                   
               
               
                           7390      7400      7410      7420      7430      7440 
               
               
                 BH 10  CCCATCAGTGGACAAATTAGATGTTCATCAAATATTACAGGGCTGCTATTAACAAGAGAT 
               
               
                        :::::  : ::::::::::: ::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CCCATTGGAGGACAAATTAGTTGTTCATCAAATATTACAGGGCTGCTATTAACAAGAGAT 
               
               
                           7530      7540      7550      7560      7570      7580 
               
               
                   
               
               
                           7450         7460      7470      7480      7490      7500 
               
               
                 BH 10  GGTGGTAATA--GCAAC-AATGAGTCCGAGATCTTCAGACCTGGAGGAGGAGATATGAGG 
               
               
                        :::::::  :  : ::: :::::  ::::: ::::::::::::::::::::::::::::: 
               
               
                 Licuw, GGTGGTACAAATGTAACTAATGACACCGAGGTCTTCAGACCTGGAGGAGGAGATATGAGG 
               
               
                           7590      7600      7610      7620      7630      7640 
               
               
                   
               
               
                              7510      7520      7530      7540      7550      7560 
               
               
                 BH 10  GACAATTGGAGAAGTGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTA 
               
               
                        :::::::::::::::::::::::::::::::::::: :::::::::::::::::::: :: 
               
               
                 Licuw, GACAATTGGAGAAGTGAATTATATAAATATAAAGTAATAAAAATTGAACCATTAGGAATA 
               
               
                           7650      7660      7670      7680      7690      7700 
               
               
                   
               
               
                              7570      7580      7590      7600      7610 
               
               
                 BH 10  GCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAG-- 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, GCACCCACCAAGGCAAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGTA 
               
               
                           7710      7720      7730      7740      7750      7760 
               
               
                   
               
               
                       7620      7630      7640      7650      7660      7670 
               
               
                 BH 10  -GAGCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATG 
               
               
                         ::::: :::::::::::::::::::::::::::::::::::::::: :::: :::: :: 
               
               
                 Licuw, GGAGCTATGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACTATGGGGGCAGTGTCATTG 
               
               
                           7770      7780      7790      7800      7810      7820 
               
               
                   
               
               
                       7680      7690      7700      7710      7720      7730 
               
               
                 BH 10  ACGCTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTG 
               
               
                        :::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::: 
               
               
                 Licuw, ACGCTGACGGTACAGGCCAGACAATTATTGTCTGGTATAGTGCAACAGCAGAACAATTTG 
               
               
                           7830      7840      7850      7860      7870      7880 
               
               
                   
               
               
                       7740      7750      7760      7770      7780      7790 
               
               
                 BH 10  CTGAGGGCTATTGAGGCGCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAG 
               
               
                        ::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CTGAGGGCTATTGAGGCGCAACAACATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAG 
               
               
                           7890      7900      7910      7920      7930      7940 
               
               
                   
               
               
                       7800      7810      7820      7830      7840      7850 
               
               
                 BH 10  CTCCAGGCAAGAATCCTGGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATT 
               
               
                        :::::::::::: :::::::::::::::::::::::: ::::::::::::::: :  ::: 
               
               
                 Licuw, CTCCAGGCAAGAGTCCTGGCTGTGGAAAGATACCTAAGGGATCAACAGCTCCTAG--ATT 
               
               
                           7950      7960      7970      7980      7990        8000 
               
               
                   
               
               
                       7860      7870      7880      7890      7900      7910 
               
               
                 BH 10  TGGGGTTGCTCTGGAAAACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGT 
               
               
                        :::::: :::::::::: :::::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, TGGGGT-GCTCTGGAAA-CTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGT  
               
               
                              8010       8020      8030      8040      8050 
               
               
                   
               
               
                       7920      7930      7940      7950      7960      7970 
               
               
                 BH 10  AATAAATCTCTGGAACAGATTTGGAATAACATGACCTGGATGGAGTGGGACAGAGAAATT 
               
               
                        ::::::::::::::: : :::::: ::::::::::::: ::: ::::::: ::::::::: 
               
               
                 Licuw, AATAAATCTCTGGAAGACATTTGGGATAACATGACCTG-ATGCAGTGGGAAAGAGAAATT 
               
               
                     8060      8070      8080      8090       8100      8110 
               
               
                   
               
               
                       7980      7990      8000      8010      8020      8030 
               
               
                 BH 10  AACAATTACACAAGCTTAATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAG 
               
               
                         :::::::::::: :  :::: :: ::::: ::::::::::::: ::::: ::::::::: 
               
               
                 Licuw, GACAATTACACAAACACAATATACACCTTACTTGAAGAATCGCAGAACCAACAAGAAAAG 
               
               
                      8120      8130      8140      8150      8160      8170 
               
               
                   
               
               
                       8040      8050      8060      8070      8080      8090 
               
               
                 BH 10  AATGAACAAGAATTATTGGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATA 
               
               
                        ::::::::::::::::: ::::: ::::: ::::::::::::::::::::::::: :::: 
               
               
                 Licuw, AATGAACAAGAATTATTAGAATTGGATAAGTGGGCAAGTTTGTGGAATTGGTTTAGCATA 
               
               
                      8180      8190      8200      8210      8220      8230 
               
               
                   
               
               
                       8100      8110      8120      8130      8140      8150 
               
               
                 BH 10  ACAAATTGGCTGTGGTATATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTA 
               
               
                        ::::: :::::::::::::::::  ::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, ACAAACTGGCTGTGGTATATAAAGATATTCATAATGATAGTAGGAGGCTTGGTAGGTTTA 
               
               
                      8240      8250      8260      8270      8280      8290 
               
               
                   
               
               
                       8160      8170      8180      8190      8200      8210 
               
               
                 BH 10  AGAATAGTTTTTGCTGTACTTTCTGTAGTGAATAGAGTTAGGCAGGGATATTCACCATTA 
               
               
                        ::::::::::::::::: :::::: ::::::::::::::::::::::::: ::::::::: 
               
               
                 Licuw, AGAATAGTTTTTGCTGTGCTTTCTATAGTGAATAGAGTTAGGCAGGGATACTCACCATTG 
               
               
                      8300      8310      8320      8330      8340      8350 
               
               
                   
               
               
                       8220      8230      8240      8250      8260      8270 
               
               
                 BH 10  TCGTTTCAGACCCACCTCCCAATCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAA 
               
               
                        :: ::::::::::  :::::: : :::::   ::::::::: ::::: ::::: :::::: 
               
               
                 Licuw, TCATTTCAGACCCGGCTCCCAGTGCCGAG---ACCCGACAG-CCCGACGGAATCGAAGAA 
               
               
                      8360      8370      8380         8390       8400      8410 
               
               
                   
               
               
                       8280      8290      8300      8310      8320      8330 
               
               
                 BH 10  GAAGGGGAGAGAGAGACAGAGACAGATCCATTCGATTAGTGAACGGATCCTTAGCACTTA 
               
               
                        ::::::::::::::::::::::::::::: ::::::::::: : :::: ::::::::::: 
               
               
                 Licuw, GAAGGGGAGAGAGAGACAGAGACAGATCCGTTCGATTAGTGGATGGATTCTTAGCACTTA 
               
               
                          8420      8430      8440      8450      8460      8470 
               
               
                   
               
               
                       8340      8350      8360      8370      8380      8390 
               
               
                 BH 10  TCTGGGACGATCTGCGGAGCCTGTGCCTCTTCAGCTACCACCGCTTGAGAGACTTACTCT 
               
               
                        ::::::: :::::::::::::::::::::::::::::::  :: :::::::::::::::: 
               
               
                 Licuw, TCTGGGAAGATCTGCGGAGCCTGTGCCTCTTCAGCTACCGGCGGTTGAGAGACTTACTCT 
               
               
                          8480      8490      8500      8510      8520      8530 
               
               
                   
               
               
                       8400      8410      8420      8430      8440      8450 
               
               
                 BH 10  TGATTGTAACGAGGATTGTGGAACTTCTGGGACGCAGGGGGTGGGAAGCCCTCAAATATT 
               
               
                        :::::: : :::::: ::::::: ::::::: : :::::::::::::   :::::::::: 
               
               
                 Licuw, TGATTGCAGCGAGGACTGTGGAAATTCTGGGGCACAGGGGGTGGGAA---CTCAAATATT 
               
               
                          8540      8550      8560      8570      8580         8590 
               
               
                   
               
               
                       8460      8470      8480      8490      8500      8510 
               
               
                 BH 10  GGTGGAATCTCCTACAGTATTGGAGTCAGGAGCTAAAGAATAGTGCTGTTAGCTTGCTCA 
               
               
                        :::::: :::::: :::::::::: :::::: :::::::::::::::::::::: ::::: 
               
               
                 Licuw, GGTGGAGTCTCCTGCAGTATTGGATTCAGGAACTAAAGAATAGTGCTGTTAGCTGGCTCA 
               
               
                             8600      8610      8620      8630      8640      8650 
               
               
                   
               
               
                       8520      8530      8540      8550      8560      8570 
               
               
                 BH 10  ATGCCACAGCTATAGCAGTAGCTGAGGGGACAGATAGGGTTATAGAAGTAGTACAAGGAG 
               
               
                        : :::::::::::::::::: :::::::::::::::::::::::::::::: :::: ::: 
               
               
                 Licuw, ACGCCACAGCTATAGCAGTAACTGAGGGGACAGATAGGGTTATAGAAGTAGCACAAAGAG 
               
               
                             8660      8670      8680      8690      8700      8710 
               
               
                   
               
               
                       8580      8590      8600      8610      8620      8630 
               
               
                 BH 10  CTTATAGAGCTATTCGCCACATACCTAGAAGAATAAGACAGGGCTTGGAAAGGATTTTGC 
               
               
                        ::::::::::::::: :::::::: ::::::::: :::::   :::::::::: :::::: 
               
               
                 Licuw, CTTATAGAGCTATTCTCCACATACATAGAAGAATTAGACA---CTTGGAAAGGCTTTTGC 
               
               
                             8720      8730      8740      8750         8760 
               
               
                   
               
               
                       8640      8650      8660      8670      8680      8690 
               
               
                 BH 10  TATAAGATGGGTGGCAAGTGGTCAAAAAGTAGTGTGGTTGGATGGCCTGCTGTAAGGGAA 
               
               
                        ::::::::::: :   ::::::::::: : ::: :::  :::::: ::::: :::::::: 
               
               
                 Licuw, TATAAGATGGGGG---AGTGGTCAAAACG-AGTATGGG-GGATGGTCTGCTATAAGGGAA 
               
               
                      8770      8780         8790       8800       8810      8820 
               
               
                   
               
               
                       8700      8710                  8720      8730      8740 
               
               
                 BH 10  AGAATGAGACGAGCTGAGCCA------------GCAGCAGATGGGGTGGGAGCAGCATCT 
               
               
                        :::::::::::::::::::::            :::::::::::: ::::: ::: :::: 
               
               
                 Licuw, AGAATGAGACGAGCTGAGCCACGAGCTGAGCCAGCAGCAGATGGG-TGGGA-CAGTATCT 
               
               
                           8830      8840      8850      8860       8870       8880 
               
               
                   
               
               
                         8750      8760      8770      8780      8790      8800 
               
               
                 BH 10  CGAGACCTAGAAAAACATGGAGCAATCACAAGTAGCAACACAGCAGCTAACAATGCTGAT 
               
               
                        :::::::: ::::::::::::  ::::::::::::::: ::::::::::  ::::::::: 
               
               
                 Licuw, CGAGACCTGGAAAAACATGGA--AATCACAAGTAGCAATACAGCAGCTACTAATGCTGAT 
               
               
                             8890      8900        8910      8920      8930 
               
               
                   
               
               
                         8810      8820      8830      8840      8850      8860 
               
               
                 BH 10  TGTGCCTGGCTAGAAGCACAAGAGGAGGAGGAGGTGGGTTTTCCAGTCACACCTCAGGTA 
               
               
                        ::::::::::::::::::::::::::::: ::::::::::::::::::: :::::::::: 
               
               
                 Licuw, TGTGCCTGGCTAGAAGCACAAGAGGAGGAAGAGGTGGGTTTTCCAGTCAGACCTCAGGTA 
               
               
                     8940      8950      8960      8970      8980      8990 
               
               
                   
               
               
                         8870      8880      8890      8900      8910      8920 
               
               
                 BH 10  CCTTTAAGACCAATGACTTACAAGGCAGCTGTAGATCTTAGCCACTTTTTAAAAGAAAAG 
               
               
                        :::::::::::::::::::::::::::::: ::::: ::::::::::::::::::::::: 
               
               
                 Licuw, CCTTTAAGACCAATGACTTACAAGGCAGCTTTAGATATTAGCCACTTTTTAAAAGAAAAG 
               
               
                     9000      9010      9020      9030      9040      9050 
               
               
                   
               
               
                         8930      8940      8950      8960      8970      8980 
               
               
                 BH 10  GGGGGACTGGAAGGGCTAATTCACTCCCAACGAAGACAAGATATCCTTGATCTGTGGATC 
               
               
                        :::::::::::::::::::::   :::::: :::::::::: :::::::::::::::::: 
               
               
                 Licuw, GGGGGACTGGAAGGGCTAATTTGGTCCCAAAGAAGACAAGAGATCCTTGATCTGTGGATC 
               
               
                     9060      9070      9080      9090      9100      9110 
               
               
                   
               
               
                         8990      9000      9010      9020      9030      9040 
               
               
                 BH 10  TACCACACACAAGGCTACTTCCCTGATTAGCAGAACTACACACCAGGGCCAGGGATCAGA 
               
               
                        :::::::::::::::::::::::::::: :::::: :::::::::::::::::::::::: 
               
               
                 Licuw, TACCACACACAAGGCTACTTCCCTGATTGGCAGAATTACACACCAGGGCCAGGGATCAGA 
               
               
                     9120      9130      9140      9150      9160      9170 
               
               
                   
               
               
                         9050      9060      9070      9080      9090      9100 
               
               
                 BH 10  TATCCACTGACCTTTGGATGGTGCTACAAGCTAGTACCAGTTGAGCCAGAGAAGTTAGAA 
               
               
                        ::::::::::::::::::::::::: :::::::::::::::::::::::::::: ::::: 
               
               
                 Licuw, TATCCACTGACCTTTGGATGGTGCTTCAAGCTAGTACCAGTTGAGCCAGAGAAGGTAGAA 
               
               
                     9180      9190      9200      9210      9220      9230 
               
               
                   
               
               
                         9110      9120      9130      9140      9150      9160 
               
               
                 BH 10  GAAGCCAACAAAGGAGAGAACACCAGCTTGTTACACCCTGTGAGCCTGCATGGAATGGAT 
               
               
                        :: :::::  ::::::::::::  ::::::::::::::: ::::::::::::: ::::: 
               
               
                 Licuw, GAGGCCAATGAAGGAGAGAACAA-AGCTTGTTACACCCTATGAGCCTGCATGGGATGGAG 
               
               
                     9240      9250      9260       9270      9280      9290 
               
               
                   
               
               
                         9170      9180      9190      9200      9210      9220 
               
               
                 BH 10  GACCCGGAGAGAGAAGTGTTAGAGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACATG 
               
               
                        ::: :::::: ::::::::::: ::::::::::::::::   :::::::::::::::::: 
               
               
                 Licuw, GACGCGGAGAAAGAAGTGTTAGTGTGGAGGTTTGACAGCAAACTAGCATTTCATCACATG 
               
               
                      9300      9310      9320      9330      9340      9350 
               
               
                   
               
               
                         9230      9240      9250      9260      9270      9280 
               
               
                 BH 10  GCCCGAGAGCTGCATCCGGAGTACTTCAAGAACTGCTGACATCGAGCTTGCTACAAGGGA 
               
               
                        ::::::::::::::::::::::::: :::  :::::::::::::::::: :::::::::: 
               
               
                 Licuw, GCCCGAGAGCTGCATCCGGAGTACTACAAAGACTGCTGACATCGAGCTTTCTACAAGGGA 
               
               
                      9360      9370      9380      9390      9400      9410 
               
               
                   
               
               
                         9290      9300       9310      9320      9330      9340 
               
               
                 BH 10  CTTTCCGCTGGGGACTTTCCAGG-AGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGAGCC 
               
               
                        ::::::::::::::::::::::: ::::::::::::::::::::::::::::::::  :: 
               
               
                 Licuw, CTTTCCGCTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGT-CC 
               
               
                      9420      9430      9440      9450      9460      9470 
               
               
                   
               
               
                          9350      9360      9370      9380      9390      9400 
               
               
                 BH 10  CTCAGATCCTGCATATAAGGAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCA 
               
               
                        ::::::: ::::::::::: :::::::::::::::::::::::::::::::::::::::: 
               
               
                 Licuw, CTCAGATGCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCA 
               
               
                       9480      9490      9500      9510      9520      9530 
               
               
                   
               
               
                          9410      9420 
               
               
                 BH 10  GATCTGAGCCTGGGAGCTC----------------------------------------- 
               
               
                        ::::::::::::::::::: 
               
               
                 Licuw, GATCTGAGCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAG 
               
               
                       9540      9550      9560      9570      9580      9590 
               
               
                   
               
               
                 BH 10  ------------------------------------------------------------ 
               
               
                   
               
               
                 Licuw, CTTGCCTTGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAG 
               
               
                       9600      9610      9620      9630      9640      9650 
               
               
                   
               
               
                 BH 10  ------------------------------------------- 
               
               
                   
               
               
                 Licuw, ATCCCTCAGACCCTTTTAGTCAGTGTGGAAAAATCTCTAGCAG 
               
               
                       9660      9670      9680      9690      9700