Abstract:
A process of providing novel compounds of Formula (I) below, which are useful as intermediates in the preparation of camptothecin and camptothecin-like compounds, ##STR1## wherein: R 1  represents alkyl, particularly methyl, R 2  represents H or alkyl, particularly methyl, R3 represents H or alkyl, particularly H; Q represents triflate or halo particularly bromo and iodo more particularly iodo and Y represents H, chloro or OR 4 , wherein R 4  represents alkyl or triflate, or particularly H.

Description:
FIELD OF THE INVENTION 
     The present invention relates to novel chemical compounds that are useful as intermediates in the preparation of camptothecin and camptothecin-like compounds and to processes for their preparation. 
     BACKGROUND OF THE INVENTION 
     Useful camptothecin and camptothecin-like compounds that can be produced using intermediates and processes of the present Invention include compounds described in U.S. Pat. No. 4,894,456 to Wall et al. issued Jan. 16, 1990; U.S. Pat. No. 4,399,282 to Miyasaka, et al. issued Aug. 16, 1983; U.S. Pat. No. 4,399,276 to Miyasaka, et al. issued Aug. 16, 1983; U.S. Pat. No. 4,943,579 to Vishnuvajjala, et al. issued Jul. 24, 1990; European Patent Application 0 321 122 A2 filed by SmithKline Beckman Corporation, and published Jun. 21, 1989; U.S. Pat. No. 4,473,692 to Miyasaka, et al. issued Sep. 25, 1984; European Patent application No. 0 325 247 A2 filed by Kabushiki Kaisha Yakult Honsha, and published Jul. 26, 1989; European Patent application 0 556 585 A2 filed by Takeda Chemical Industries, and published Aug. 25, 1993; U.S. Pat. No. 4,981,968 to Wall, et al. issued Jan. 1, 1991; U.S. Pat. No. 5,049,668 to Wall, et al. issued Sep. 17, 1991; U.S. Pat. No. 5,180,722 to Wall, et al. issued Jan. 19, 1993 and European Patent application 0 540 099 A1, filed by Glaxo Inc., and published May 5, 1993. 
     Previous methods used in the preparation of camptothecin and camptothecin-like compounds employ resolutions or chiral auxiliaries to obtain enantiomerically enriched intermediates. A problem with these methods is that a resolution necessitates discarding half of the racemic material and a chiral auxiliary requires utilizing stoichiometric amounts of a chiral subunit to enantioselectively install the chiral center. 
     The present Invention describes a method for producing enantiomerically enriched intermediates useful for the preparation of camptothecin and camptothecin-like compounds using a process of catalytic asymmetric induction. In this process, a chiral catalyst used in sub-stoichiometric amounts influences the stereoselective formation of the chiral center. Additionally, the process continually regenerates the chiral catalyst preventing waste. 
     SUMMARY OF THE INVENTION 
     One aspect of the present invention is a process of providing novel compounds of Formula (I), which are useful as intermediates in the preparation of camptothecin and camptothecin-like compounds, ##STR2## wherein: R 1  represents alkyl, particularly methyl, R 2  represents H or alkyl, particularly methyl, R 3  represents H or alkyl, particularly H; Q represents triflate or halo particularly bromo and iodo more particularly iodo and Y represents chloro, H or OR 4 , wherein R 4  represents alkyl, triflate, or H, wherein Y is particularly H. 
     In addition to a process for manufacturing the compounds of Formula (I), other aspects of the invention include the compounds of Formula (I) and various intermediates useful in producing the compounds of Formula (I). Other aspects and advantages of the present invention will become apparent from a review of the detailed description below. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The term &#34;alkyl&#34; as used herein means, a linear or branched alkyl group with from 1 to about 8 carbon atoms, such as, but not limited to, methyl, ethyl, propyl, isopropyl, n-butyl, tert-butyl, hexyl and octyl. The terms &#34;halo&#34; and &#34;halogen&#34; as used herein refers to a substituent which may be fluoro, chloro, bromo, or iodo. The term &#34;triflate&#34; as used herein refers to trifluoromethanesulphonate. The designation &#34;C&#34; as used herein means centigrade. The term &#34;ambient temperature&#34; as used herein means from about 20° C. to about 30° C. 
     Compounds of the present Invention have 1 or more asymmetric carbon atoms that form enantiomeric arrangements, i.e., &#34;R&#34; and &#34;S&#34; configurations. The present Invention includes all enantiomeric forms and any combinations of these forms. For simplicity, where no specific configuration is depicted in the structural formulas, it is to be understood that both enantiomeric forms and mixtures thereof are represented. Unless noted otherwise, the nomenclature convention, &#34;(R)&#34; and &#34;(S)&#34; denote essentially optically pure R and S enantiomers respectively. Also included in the present Invention are other forms of the compounds including: solvates, hydrates, various polymorphs and the like. 
     Acceptable salts include, but are not limited to salts with inorganic acids and bases such as hydrochloride, sulfate, phosphate, diphosphate, hydrobromide and nitrate or salts with organic acids such as acetate, malate, maleate, fumarate, tartrate, succinate, citrate, lactate, methanesulfonate, p-toluenesulfonate, palmoate, salicylate, oxalic and stearate. For further examples of acceptable salts see, &#34;Pharmaceutical Salts,&#34; J. Pharm. Sci., 66(1), 1 (1977). 
     Thus, one aspect of the present Invention provides a process for preparing compounds of Formula (V); ##STR3## which comprises dihydroxlating a compound of Formula (III), ##STR4## using a catalytic asymmetric dihydroxylation reaction. The reaction is carried out in the presence of an osmium catalyst (e.g., potassium osmate (VI) dihydrate, osmium chloride hydrate or osmium tetroxide), a chiral tertiary amine catalyst (e.g., derivatives of the cinchona alkaloids such as 2,5-diphenyl-4,6-bis(9-O-dihydroquinidyl)pyrimidine), a stoichiometric oxidizing reagent (e.g., potassium ferricyanide, hydrogen peroxide or N-methylmorpholine N-oxide), and a primary amide (e.g., methanesulfonamide) under basic conditions (e.g. potassium carbonate) in an aqueous mixture containing a polar protic solvent (e.g., t-butanol, i-propanol, n-propanol). The reaction should be carried out at a temperature of between about 0° C. to about 25° C. for about 24 to about 48 hours. Variations on these conditions will be apparent from the literature on the catalytic asymmetric dihydroxylation reaction. See, e.g., K. B. Sharpless et al., J. Org. Chem. 58, 3785-3786 (1993). 
     Alternatively the compound of Formula III is oxidized to a compound of Formula V in an achiral dihydroxylation reaction to yield a racemic cis-diol which is then resolved enzymatically to give the enantiomerically enriched compound of Formula V. See R. Larock, Comprehensive Organic Transformations, 493-496 (1989). The resolution reaction may be carried out in the presence of an acylating enzyme such as pancreatic lipases, pseudomonas fluorenscens lipases, C. cylindracea lipases, Chromobacterium viscosum lipases and Aspergillus niger lipases in the presence of an acylating agent such as vinyl acetate at a temperature of between about 0° C. to ambient temperature for about 2 to about 48 hours. Variations on these conditions will be apparent from A. Ktibanov, Asymmetric Transformations Catalyzed by Enzymes in Organic Solvents, Acc. Chem Res. 1990, 23, 114-120. 
     Compounds of Formula (Ill) may be prepared by isomerizing a compound of Formula (IV). ##STR5## 
     The isomerization is carried out under standard conditions used to isomerize double bonds, for example, in the presence of a metal catalyst (e.g. a rhodium catalyst such as tris(triphenylphosphine)rhodium(l) chloride under basic conditions in a polar aprotic solvent (e.g., acetonitrile or dimethylformamide) or a polar protic solvent (e.g., n-propanol, i-propanol, or t-butanol). The reaction should be carried out in an inert atmosphere, such as under nitrogen or argon gas in a suitable reaction vessel equipped with an overhead mechanical stirrer and water cooled condenser. The reaction mixture may be heated to a temperature between about 50° to about 110° C. for about 1 to about 24 hours. Variations on these conditions will be apparent from Grieco et. al, R. Heck, Palladium Reagents in Organic Syntheses, 19-22 (1990) and Journal of the American Chemical Society, 98:22, 7102-7104, 1976. 
     Compounds of Formula (IV) may be prepared by cyclizing a compound of Formula (II). ##STR6## 
     The compounds of Formula (II) may be cyclized by an intramolecular Heck reaction. The reaction may be carried out in the presence of a palladium catalyst (e.g., palladium(II) acetate) under basic conditions in a polar aprotic solvent (e.g., acetonitrile, or N,N-dimethylformamide) or a polar protic solvent (e.g., n-propanol, i-propanol, or t-butanol). A phase transfer catalyst such as a tetraalkylammonium halide salt (e.g., tetrabutyl ammonium chloride, tetrabutyl ammonium bromide, or tetrabutyl ammonium iodide) may be included especially when a polar aprotic solvent is used. A ligand for the palladium catalyst may also be included such as a triphenylphosphine, tri-o-tolyphosphine, tri-m-tolyphosphine or tri-p-tolyphosphine. The reaction should be carried out in an inert atmosphere, such as under nitrogen or argon gas in a suitable reaction vessel equipped with an overhead mechanical stirrer and water cooled condenser. The reaction mixture may be heated to a temperature between about 50° to about 110° C. for about 1 to about 24 hours. Variations on these conditions will be apparent from the literature on the Heck reaction. See, e.g., R. Grigg et at. Tetrahedron 46, 4003-4008 (1990). 
     The intermediates of Formula (V) may be oxidized to yield a compound of Formula (I). ##STR7## 
     The reaction is carried out in the presence of an oxidizing reagent (e.g., iodine) in a polar protic medium (e.g., aqueous methanol, aqueous t-butanol or aqueous n-propanol) in the presence of a base (e.g., calcium carbonate) at a temperature of from about 0° C. to about 25° C. 
     The starting material, compounds of Formula II, may be produced by sequentially formylating and halogenating a halo-alkoxy-pyridine such as 2-methoxypyridine available from the Aldrich Chemical Company, Inc., 1001 West Saint Paul Avenue, Milwaukee, Wis. 53233, producing a halopyridinecarboxaldehyde. Formylation at the 3-position is disclosed in D. Comins and M. Killpack, J. Org. Chem. 55, 68-73 (1990). If an alkoxy-pyridine is being utilized the formylation may be carried out by deprotonation at the 3-position with a base such as t-butyllithium in tetrahydrofuran or 1,2-dimethoxyethane at a temperature at least as low as about -60° C. The C-3-lithiated pyridine intermediate is then alkylated with a formamide such as N-formyl-N,N&#39;,N&#39;-trimethylethylenediamine at a temperature of between about -60° C. and -20° C. The intermediate aminoalkoxy species is deprotonated at the C-4 position using a base such as n-butyllithium. The C-4-lithiated-pyridine intermediate can then be halogenated by mixing the intermediate with a solution of iodine or bromine in a polar or nonpolar organic solvent, preferably at a temperature of at least as low as about -60° C., to form an alkoxyaldehyde. The alkoxyaldehyde is then reduced in an alcoholic acidic media in the presence of a trialkylsilane to yield an alkoxymethylpyridine. The acid should be a strong non-oxidizing protic acid (e.g., sulfuric acid or trifluoroacetic acid) or a Lewis acid (e.g., boron trifluoride etherate) or an activated silylating reagent (e.g., trimethylsilyltrifluoromethanesulfonate). At least about 2 molar equivalents of an unsaturated alcohol (e.g., crotyl alcohol) should be included to convert the aldehyde to an ether of Formula (II). Conditions and variations on the silane reduction of aldehydes is described by M. Doyle et al., J. Am. Chem. Soc. 94:10, 3659-3661 (1972). The above reactions are further described in U.S. Pat. No. 5,254,690 to Comins, et al. issued Oct. 19, 1993 and incorporated herein by reference. 
     In a further particular aspect of the Invention, intermediates of Formula (III) are prepared by a single reaction comprising cyclizing and isomerizing a compound of Formula II by an intramolecular Heck reaction. The Heck reaction is carried out in the presence of a palladium catalyst (e.g., palladium IIacetate) under basic conditions (e.g., potassium carbonate) in a polar aprotic solvent (e.g., acetonitrile or dimethylformamide) or in a polar protic solvent (e.g., n-propanol, i-propanol, or t-butanol). A phase transfer catalyst such as a tetraalkylammonium halide salt (e.g. tetrabutyl ammonium chloride, tetrabutyl ammonium bromide, or tetrabutyl ammonium iodide) should be included when a polar-aprotic solvent is used. The reaction is carried out in an inert atmosphere, such as under nitrogen or argon gas in a suitable reaction vessel equipped with an overhead mechanical stirrer and water cooled condenser. The reaction mixture may be heated to a temperature of between about 50° C. to about 110° C. for about 1 to about 24 hours. Variations on these conditions will be apparent from the literature on the Heck reaction. See, e.g., R. Grigg et al. Tetrahedron 46, 4003-4008 (1990 ). 
     In a still further particular aspect of the Invention, compounds of Formula (I) are prepared by a two-step one-pot sequence of reactions comprising: dihydroxylating and oxidizing the compounds of Formula (III). The dihydroxylation is a catalytic asymmetric dihydroxylation reaction carried out in the presence of an osmium catalyst (e.g., potassium osmate (VI) dihydrate, osmium (III) chloride hydrate or osmium tetroxide), a chiral tertiary amine catalyst (e.g., derivatives of the cinchona alkaloids), a stoichiometric oxidizing reagent (e.g., potassium ferricyanide, hydrogen peroxide or N-methylmorpholine N-oxide), a primary amide (e.g., methanesulfonamide), under basic conditions (e.g., potassium carbonate) in an aqueous mixture containing a polar protic alcohol (e.g., t-butanol, i-propanol, n-propanol). The reaction is be carried out at a temperature between about 0° C. to about 25° C. for about 24 to about 48 hours. Variations on these conditions will be apparent from literature on the catalytic asymmetric dihydroxylation reaction. See, e.g., K. B. Sharpless et al., J. Org. Chem. 58, 3785-3786 (1993). The following step, an oxidation, is carried out on the above reaction mixture in the presence of an oxidizing reagent (e.g., iodine, chromium trioxide or pyridine sulfur trioxide) and a base (e.g., calcium carbonate or triethylamine) at a temperature between about 0° C. to about 25° C. 
     Thus, progressing compounds of Formula (II) to compounds of Formula (I) through the intermediate compounds of Formula (III) is schematically represented by the following reaction scheme: ##STR8## 
     In a still further aspect of the Invention, the compounds of Formula (I) are prepared by a four-step one-pot sequence of reactions comprising i) cyclizing ii) isomerizing iii) dihydroxlating and iv) oxidizing a compound of Formula (II) as described herein. 
     A still further aspect of the present Invention is a method of removing heavy metal contamination from organic compounds produced by processes described herein. This heavy metal removal process is, moreover, applicable to other organic compounds. Heavy metals able to be removed employing this process include but are not limited to: palladium, osmium, iron, lead, cobalt, rhodium, chromium, manganese, mercury, copper, titanium, zinc, nickel and platinum. An example of the removal of the heavy metal catalyst palladium is exemplified herein. 
     The compounds of Formula (I), (II), (III), (IV) and (V) are novel and represent a still further aspect of the Invention. 
     The compounds of Formula (I) are useful as intermediates in the preparation of camptothecin and camptothecin-like derivatives such as those defined and described in European Patent application 0 540 099 A1, filed by Glaxo Inc., and published May 5, 1993 and incorporated herein by reference. Thus, for example, a compound of Formula (I) may be demethylated under acidic or basic conditions to yield a compound of Formula (VI). ##STR9## 
     Racemic compounds of Formula (VI) are known and have been described in World Patent Application WO 92/11263, filed by North Carolina State University, and published Jul. 9, 1992, having utility as intermediates for the preparation of camptothecin derivatives. 
     A typical preparation and heavy metal contamination removal from a camptothecin derivative, utilizing an intermediate of Formula (I) is exemplified herein. 
     EXAMPLES 
     The following examples illustrate various aspects of the present Invention, but should not be construed as limitations. The symbols, conventions and nomenclature not specifically defined below are consistent with those used in the contemporary chemical literature, for example the Journal of the American Chemical Society. 
     In the examples that follow: &#34;mg&#34; means milligram(s), &#34;M&#34; means molar, &#34;mL&#34; means milliliter(s), &#34;mmol&#34; means millimole(s), &#34;L&#34; means liter(s), &#34;mol&#34; means mole(s), &#34;g&#34; means gram(s), &#34;TLC&#34; means thin layer chromatography, &#34;HPLC&#34; means high pressure liquid chromotography, &#34;mm&#34; means millimole(s), &#34;mp&#34; means melting point, &#34;MHz&#34; means Megahertz, &#34; 1  H-NMR&#34; means proton nuclear magnetic resonance, &#34;Hz&#34; means Hertz, &#34;hr &#34; or &#34;h&#34; means hour(s) and &#34;N&#34; means normal. Also included in the following examples is terminology commonplace with the use of a proton nuclear magnetic resonance spectrometer. 
     Unless otherwise noted all starting materials were obtained from commercial suppliers and used without further purification. All reactions involving oxygen or moisture-sensitive compounds were performed under a dry N 2  atmosphere. All reactions and chromatography fractions were analyzed by thin-layer chromatography on silica gel plates, visualized with UV light and I 2  stain. 
       1  H-NMR spectra were measured in CDCl 3  or DMSO-d 6  using either a Varian Gemini-200 MHz or a Bruker AMX-400 MHz instrument. J values are reported in Hertz. Chemical shifts are expressed in ppm in reference to an internal standard such as CHCl 3  or DMSO. Apparent multiplicities are designated as s, singlet; d, doublet; t, triplet; q, quartet; m, multiplet; b, broad. All mass spectra were taken in the positive ion mode under chemical ionization (CI), electron impact (EI), or by fast-atom bombardment (FAB). Melting points were determined on a Digital melting point apparatus, electrothermal series 1A9000, model 9200 and are uncorrected. Elemental analyses were performed by Atlantic Microlab, Norcross, Ga. 
     EXAMPLE 1 
     4-Iodo-2-methoxy-pyridine-3-carbaldehyde 
     A 5-liter 3-necked round-bottom flask equipped with an overhead mechanical stirrer and under nitrogen, is charged with tetrahydrofuran (1 L) and cooled to -78° C. To this stirred solution is added tert-butyllithium (1.7M in pentane, 800 mL, 1.36 mol) via canula followed by 2-methoxypyridine (132.2 g, 1.21 mol) in tetrahydrofuran at -78° C. The mixture is allowed to stir for one hr at -78° C. To the mixture is added N-formyl-N,N&#39;,N&#39;-trimethylethylenediamine (176 mL, 1.37 mol) dropwise at -78° C. (prepared as in Comins, D. L.; Baevsky, M. F.; Hong, H. J. Am. Chem. Soc. 1992, 114, 10972). The reaction mixture is stirred for ca. 30 min at -78° C. before warming to -23° C. over ca. 30 min. To the mixture at -23° C. is added ethylene glycol dimethyl ether (1 L) followed by n-butyllithium (2.5M in hexanes, 800 mL, 2.0 mol). The resultant mixture is stirred for ca. 2 h during which time the reaction mixture turns deep green. A 12 liter 4-neck round bottom flask is equipped with an overhead mechanical stirrer under nitrogen, the 12 liter flask is charged with iodine (571 g, 2.25 mol) and ethylene glycol dimethyl ether (2 L) and the resultant solution is cooled to -78° C. The contents of the 5 liter flask are transferred via canula to the mixture of iodine and ethylene glycol dimethyl ether in the 12 liter flask at -78° C. After the addition is complete, the reaction mixture is stirred for 1 hr at -78° C. The cooling bath is removed and the mixture is allowed to warm to ca. 0° C. then treated with 2 L of water and 2 L of 1N hydrochloric acid. Methyl t-butyl ether (2 L) is added and the layers are separated. The aqueous layer is extracted with 2×1 L of methyl t-butyl ether. The combined organic extracts are washed with 1.2 L of saturated sodium thiosulfate solution followed by 1.2 L of saturated sodium chloride solution. The organic extracts are dried over sodium sulfate, filtered, and concentrated in vacuo to give a thick slurry. To the slurry is added 1 L of hexane resulting in the generation of additional precipitate. The mixture is cooled in an ice/water bath for ca. 30 min then filtered yielding 4-iodo-2-methoxy-pyridine-3-carbaldehyde. The filtrate is reconcentrated to a slurry and treated with hexane to generate additional precipitate again yielding 4-iodo-2-methoxy-pyridine-3-carbaldehyde. Chromatography (silica gel, 10% ethyl acetate/hexane) yields an analytical sample as a bright yellow solid: mp 98°-99° C. 1H-NMR (400 MHz, CDCl 3 ) 10.21 (s, 1H), 7.86 (d, J=5 Hz, 1H), 7.54 (d, J=5 Hz, 1H), 4.06 (s, 3H); IR (CHCl 3 ) 1710, 1560, 1470, 1380, 1305, 1260, 1025 cm -1  ; Elemental analysis: calculated for C 7  H 6  NO 2  I: C 31.97%, H 2.30, N 5.32, 1 48.25;  Found: C 32.06, H 2.35; N 5.25, I 48.35. 
     EXAMPLE 2 
     3-(but-2-enyloxymethyl)-4-iodo-2-methoxy-pyridine 
     A 500 mL 3-necked round-bottom flask equipped with an overhead mechanical stirrer and under nitrogen, is charged with 4-iodo-2-methoxy-pyridine-3-carbaldehyde (75.0 g, 0.29 mol) as prepared in example 1, crotyl alcohol (75 mL, 0.88 mol), and triethylsilane (70 mL, 0.44 mol). To the stirred suspension at 0° C. is added trifluoroacetic acid (175 mL, 2.27 mol) dropwise via an addition funnel. The resulting solution is stirred at about 22° C. for approximately 12 hr. The reaction mixture is slowly poured into a rapidly stirring saturated sodium bicarbonate solution (2 L). The mixture is extracted with 3×500 mL of hexane. The combined organic layers are dried over sodium sulfate, filtered, and concentrated in vacuo to give an oil. Purification of this oil by vacuum distillation (ca. 0.4 mm Hg, ca. 120°-130° C.) yields 3-(but-2-enyloxymethyl)-4-iodo-2-methoxy-pyridine as a pale yellow oil:  1  H-NMR (400  MHz, CDCl 3 ) δ7.69 (d, J=5 Hz, 1H), 7.34 (d, J=5 Hz, 1H), 5.71 (m, 2H), 4.58 (s, 2H), 4.02 (d, J=1 Hz, 2H), 3.94 (s, 3H), 1.72(d, J=6 Hz, 3H); IR (neat) 2948, 2859, 1561, 1459, 1381, 1361, 1301, 1233, 1169, 1094, 1052 cm -1  ; Elemental analysis: calculated for C 11  H 14  NO 2  I: C 41.40, H 4.42, N 4.39, I 39.76; Found: C 41.31, H 4.45, N 4.37, I 39.71. 
     EXAMPLE 3 
     4-ethylidene-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine; 4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine; 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol; 4(S)-4-ethyl-4-hydroxy-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one 
     A 5 liter 3-necked round-bottom flask is equipped with an overhead mechanical stirrer and under nitrogen, is charged with 1-propanol (1.0 L), potassium carbonate (45.0 g, 0.33 mol), 3-(but-2-enyloxymethyl)-4-iodo-2-methoxy-pyridine (49.41 g, 0.16 mol) as prepared in Example 2, and palladium(II) acetate (1.42 g, 6.33 mmol). The resulting slurry is heated at reflux for approximately 2 hr. During this time the color of the reaction mixture turns dark brown then light gray. A 150 mL aliquot of the reaction mixture is removed to identify 4-ethylidene-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine and 4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine. 
     This aliquot is diluted with 200 mL of tert-butyl methyl ether, filtered, and concentrated in vacuo affording a colorless oil. Chromatography on silica gel (5% ethyl acetate: hexanes) yields 4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine.  1  H-NMR (400 MHz, CDCl 3 ) δ8.04 (d, J=5.4 Hz, 1H), 6.65 (d, J=5.4 Hz, 1H), 6.54 (s, 1H), 5.04 (s, 2H), 3.94 (s, 3H), 2.32 (m, 2H), 0.97 (t, J=7.2 Hz, 3H), HRMS (EI + ): calc for C 11  H 13  NO 2  : 191.0946, Found: 191.0952. IR(neat) 3450, 2962, 1731, 1602, 1581, 1454, 1390, 1362, 1313, 1267 cm -1 . Further elution gives 4-ethylidene-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine:  1  H-NMR (400 MHz, CDCl 3 ) δ7.97 (d, J=6 Hz, 1H), 7.04 (d, J=6 Hz, 1H), 6.33 (q, J=7 Hz, 1H), 4.68 (s, 2H), 4.48 (s, 2H), 3.94 (s, 3H), 1.82 (d, J=2 Hz, 3H); MS (EI) 191 (M + ). 
     The reaction mixture is further treated with potassium ferricyanideIII (130 g, 0.40 mol), potassium carbonate (55.4 g, 0.40 mol),hydroquinidine 2,5-diphenyl-4,6-pyrimidinediyl diether (available from the Aldrich Chemical Company, Inc., 1001 West Saint Paul Avenue, Milwaukee, Wis. 53233) (1.16 g, 1.32 mmol), water (0.85 L) and methanesulfonamide (12.5 g, 0.13 mol). After cooling the mixture to 0° C., potassium osmate(VI) dihydrate (97 mg, 0.26 mmol) is added and the mixture is stirred for 2 days at 0° C. 
     A 300 ml aliquot of the reaction mixture is removed for identification of 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol. The aliquot is diluted with 150 mL of water and extracted with 3×50 mL of methylene chloride. The combined organic layers are washed with 2 N potassium hydroxide. The aqueous layer is extracted with 100 mL of methylene chloride. The combined organic extracts are dried over sodium sulfate, filtered, and concentrated in vacuo giving a crude product. Chromatography of this material on silica gel using 5% methanol:methylene chloride provides an analytical sample of 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol: mp. 106°-107° C. (dec);  1  H-NMR (400 MHz, CDCl 3 ) δ8.11 (d, J=5 Hz, 1H), 7.14 (d, J=5 Hz, 1H), 5.21 (d, J=5 Hz, 1H), 4.84 (d, J=16 Hz, 1H), 4.66 (d, J=16 Hz, 1H), 3.98 (s, 3H), 3.67 (d, J=5 Hz,  1H), 2.60 (s, 1H), 1.87 (q, J=8 Hz, 2H), 0.93 (t, J=8 Hz, 3H); IR 3450, 2948, 2375, 2360, 1604, 1580, 1459, 1403, 1368, 1267 cm -1  ; Calculated for C 11  H 14  NO 4  : 58.66% C, 6.71% H, 6.22% N; Found 58.75% C, 6.75% H, 6.26% N; [α] D   22  =-59.2[c. 0.62, CHCl 3  ]. 
     The reaction mixture is further treated with iodine (280 g, 1.10 mol) and calcium carbonate (54 g, 0.54 mol) and allowed to stir 2 days at about 22° C. The reaction mixture is cooled to 0° C. and sodium sulfite (150 g, 1.19 mol) is added. After filtering the slurry through Celite 545® the filtrate is extracted with methylene chloride (3×200 mL) and the combined organic extracts are washed with brine. The organic phase is dried with sodium sulfate, filtered and then concentrated to an oil. The crude material is chromatographed (silica gel, 3% methanol/methylene chloride) to yield 4(S)-4-ethyl-4-hydroxy-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one as amber colored oil:  1  H-NMR (400 MHz, CDCl 3 ) δ8.21 (d, J=5 Hz, 1H), 7.16 (d, J=5 Hz, 1H), 5.58 (d, J=16 Hz, 1H), 5.27 (d, J= 16 Hz, 1H), 3.99 (s, 3H), 3.62 (s, 1H), 1.80 (m, 2H), 0.96 (t, J=7 Hz, 3H); Calculated for C 11  H 13  NO 4  : C 59.19, H 5.87, N 6.27; Found: C 59.11, H 5.91, N 6.16; IR (neat) 3480, 2977, 2952, 2360, 1744, 1603, 1592, 1457, 1378, 1379 cm -1  ; [α] D   22  +85.97°[c 0.677, CHCl 3  ]. Optical purity is determined to be a S/R ration of 10:1 by chiral HPLC: 3% ethanol/hexane, 26° C., 1 mL/min., λ=300 nm, Chiralcel-OD column 250×4.6 mm. i.d. 
     EXAMPLE 4 
     6-chloro-4-iodo-2-methoxy-3-(2-butenyloxymethyl)pyridine 
     A 3-necked 500-mL round-bottom flask is equipped with a mechanical stirrer. Under nitrogen, the flask is charged with 6-chloro-4-iodo-2-methoxypyridine-3-carbaldehyde (53.0 g, 178 mmol) obtained as described in U.S. Pat. No. 5,254,690 to Comins et al. issued Oct. 19, 1993 and incorporated herein by reference, triethylsilane (42.7 mL, 267 mmol) and crotyl alcohol (60.8 mL, 713 mmol). The slurry is cooled to 0° C. and trifluoroacetic acid (90.0 mL, 1.17 mmol) is added over 1 h. The reaction is allowed to warm to ambient temperature and stirred for 16 h. The reaction mixture is slowly poured into a rapidly stirred solution of saturated sodium bicarbonate in water (1.5 L). The mixture is extracted with methyl t-butyl ether (4×300 mL). The combined organic layers are washed with brine (700 mL) and dried over anhydrous sodium sulfate (150 g). The drying agent is removed by filtration and the solvents are removed in vacuo to afford 6-chloro-4-iodo-2-methoxy-3-(2-butenyloxymethyl)pyridine as crude product. The product can be purified by vacuum distillation to provide 6-chloro-4-iodo-2-methoxy-3-(2-butenyloxymethyl)pyridine as a light yellow oil.  1  H NMR (200 MHz, CDCL 3 ) δ1.75 (d, J=6.1 Hz, 3H), 3.92 (s, 3H), 4.00 (m, 2H), 4.59 (s, 2H), 5.74 (m, 1H), 5.75 (m, 1H), 7.39 (s, 1H). 
     EXAMPLE 5. 
     4-ethylidene-6-chloro-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine, 4-ethyl-6-chloro-8-methoxy-1H-pyrano[3,4-c]pyridine, 3(R)-4(S)-4-ethyl-6-chloro-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol, 4(S)-4-ethyl-4-hydroxy-6-chloro-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one 
     A 2-liter 3-necked round-bottom flask equipped with an overhead stirrer and under nitrogen, is charged with 6-chloro-4-iodo-2-methoxy-3-(2-butenyloxymethyl)pyridine (26.8 g, 75.6 mmol), prepared as in example 4, anhydrous potassium carbonate (22.0 g, 159 mmol), palladium(II) acetate (697 mg, 2.98 mmol), and 500 mL of 1-propanol. The mixture is brought to reflux for 1.5 hr. The reaction turned from an initial brown color to gray at approximately 1 hr. The reaction is allowed to cool to approximately 20° C. and a 166 mL aliquot of the reaction mixture is removed for purification and characterization of the initial products. 
     The aliquot (166 mL) taken above is diluted with 250 mL of tert-butyl methyl ether and stirred at ambient temperature for 20 min. The precipitates are removed by suction filtration and the filtrate is concentrated to give a yellow liquid.  1  H NMR (400 MHz, CDCl 3 ) indicates 4-ethyl-6-chloro-8-methoxy-1H-pyrano[3,4-c]pyridine and 4-ethylidene-6-chloro-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine and chromatography on silica gel eluting with 3-5% EtOAc in hexane affords 4-ethylidene-6-chloro-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine as a white solid:  1  H NMR (400 MHz, CDCl 3 ) δ1.85 (d, J=7.2 Hz, 3H), 3.97 (s, 3H), 4.48 (s, 2H), 4.66 (s, 2H), 6.35 (q, J=7.2 Hz, 1H), 7.10 (s, 1H). In addition crude 4-ethyl-6-chloro-8-methoxy-1H-pyrano[3,4 -c]pyridine is obtained and can be further purified by chromatography on silica gel. Elution with 2% EtOAc in hexane affords an analytical sample of 4-ethyl-6-chloro-8-methoxy-1H-pyrano[3,4-c]pyridine as a yellow liquid:  1  H NMR (400 MHz, CDCl 3 ) δ1.13 (t, J=7.2 Hz, 3H), 2.26 (q, J=7.2 Hz, 3H), 3.97 (s, 3H), 5.03 (s, 2H), 6.56 (s, 1H), 6.66 (s, 1H). Elemental analysis: Calculated for C 11  H 12  ClNO 2  : C 58.55, H 5.36, N 6.21, Cl 15.71. Found: C 58.65, H 5.39,N 6.22, Cl 15.63. 
     The rest of the reaction mixture is cooled to 0° C., followed by successive addition of potassium ferricyanide(III) (49.8 g, 151 mmol), anhydrous potassium carbonate (21.1 g, 153 mmol), hydroquinidine 2,5-diphenyl-4,6-pyrimidinediyl diether (448 mg, 0.502 mmol) (available from the Aldrich Chemical Company, Inc., 1001 West Saint Paul Avenue, Milwaukee Wis. 53233), methanesulfonamide (4.77 g, 50.4 mmol) and potassium osmate (VI) dihydrate (37.1 mg, 0.10 mmol). The brown slurry is stirred vigorously at 0° C. for ca. 58 hr. At this stage, a ca. 150 mL aliquot of the reaction mixture is taken for purification and characterization of the intermediate product. The aliquot (150 mL) taken from the reaction is treated with 25 g of sodium sulfite and stirred for 20 min. The green mixture is diluted with water (200 mL) and extracted with CH 2  Cl 2  (4×150 mL). The combined extracts are successively washed with 2N aq KOH (150 mL) and brine (300 mL), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo to give a residue. Chromatography on silica gel eluting with 5% MeOH in CH 2  Cl 2  affords 3(R)-4(S)-4-ethyl-6-chloro-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol as a white solid. mp 164°-166° C. [α] D  -63.1° (c 1.30, MeOH).  1  H NMR (400 MHz, CDCl 3 ) δ0.84 (t, J=7.5 Hz, 3H), 1.71 (q, J=7.5 Hz, 3H), 2.72 (s, 1H), 3.60 (d, J=4.7 Hz, 1H), 3.88 (s, 3H), 4.59 (ABq, JAB=16.3 Hz, Δv=57.1 Hz, 2H), 5.10 (d, J=4.7 Hz, 1H), 7.08 (s, 1H). Elemental analysis: Calculated for C 11  H 14  ClNO 4  : C 50.88, H 5.43, N 5.39, Cl 13.65. Found: C 50.98, H 5.41, N 5.34, Cl 13.70. 
     The rest of the reaction mixture is treated with iodine (95.9 g, 378 mmol) and calcium carbonate (18.9 g, 189 mmol). The mixture is warmed to ambient temperature and stirred for 48 hr. After being treated with 45 g of sodium sulfite in 4 portions over 40 min, the brown mixture is stirred for 1 hr. and turned green. Filtration of the reaction mixture by suction through a short pad of Celite 545® and washing with CH 2  Cl 2  (5×300 mL) gives a two-phase mixture. The mixture is extracted with CH 2  Cl 2  (3×500 mL). The combined organic phases are washed with brine (1 L), dried over anhydrous sodium sulfate and concentrated in vacuo to give a yellow oil which partially solidifies upon standing. The oil is chromatographed on silica gel. Elution with 2% MeOH in CH 2  Cl 2  affords 4(S)-4-ethyl-4-hydroxy-6-chloro-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one which is a semi-solid at approximately 25° C.  1  H NMR shift study with Eu(hfc) 3  indicates an 8.5:1 ratio of the S: R enantiomers. The absolute stereochemistry is determined by transformation to a known compound (vide infra). [α] D  +62.4° (c 1.60, CHCl 3 ).  1  H NMR (400 MHz, CDCl 3 ) δ0.99 (t, J=7.4 Hz, 3H), 1.82 (q, J=7.4 Hz, 2H), 3.68 (s, 1H), 4.03 (s, 3H), 5.40 (ABq, JAB=15.6 Hz, Δv=120 Hz, 2H), 7.22 (s, 1H). Elemental analysis: Calculated for C 11  H 12  ClNO 4  : C 51.28, H 4.69, N 5.44, Cl 13.76. Found: C 51.20, H 4.68, N 5.38, Cl 13.84. 
     EXAMPLE 6 
     4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine 
     A 3-liter 3-necked round-bottom flask is equipped with a heating mantle monitored by a J-KEM thermocouple and an overhead stirrer; under nitrogen, the flask is charged with 3-(but-2-enyloxymethyl)-4-iodo-2-methoxy-pyridine (30.0 g, 93.9 mmol), as prepared in example 2, and 939 mL (0.1M) of N,N-dimethylformamide, followed by successive addition of anhydrous potassium carbonate (26.0 g, 188 mmol), palladium(II) acetate (422 mg, 1.88 mmol) and tetrabutylammonium chloride (13.1 g, 47.0 mmol). The light brown mixture is brought to 90° C. and stirred for 30 min. The now dark brown mixture is allowed to cool to 85° C. and stirring is continued for 20 h when TLC (10% EtOAc/hexane) indicates complete reaction. After being cooled to ambient temperature, the mixture is filtered through Celite 545® (80 g), washed with MTBE (0.5 L) and the filtrate is poured into 800 mL of ice water and extracted with MTBE (3×600 mL). The combined extracts are successively washed with water (700 mL) and brine (700 mL), dried over anhydrous Na 2  SO 4  (150 g). Evaporation in vacuo yields crude 4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine  1  H NMR (400 MHz) indicates an endo/exo ratio of 8:1. Vacuum distillation at, ca. 112°-120° C. yields 4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine as an 11:1 endo/exo mixture.  1  H-NMR (400 MHz, CDCl 3 ) δ8.04 (d, J=5.4 Hz, 1H), 6.65 (d, J=5.4 Hz, 1H), 6.54 (s, 1H), 5.04 (s, 2H), 3.94 (s, 3H), 2.32 (m, 2H), 0.97 (t, J=7.2 Hz, 3H), HRMS (EI+): calc for C 11  H 13  NO 2  : 191.0946, Found: 191.0952. IR 3450, 2962, 1731, 1602, 1581, 1454, 1390, 1362, 1313, 1267 cm -1   
     EXAMPLE 7 
     4(S)-4-ethyl-4-hydroxy-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one 
     A 2-liter 3-necked round-bottom flask equipped with an overhead stirrer and a J-KEM thermocouple is successively charged with 200 mL of t-butyl alcohol, 261 mL water, potassium ferricyanide(III) (51.7 g, 157 mmol), potassium carbonate powder (21.7 g, 157 mmol), potassium osmate (VI) dihydrate (38.5 mg, 0.105 mmol), hydroquinidine 2,5-diphenyl-4,6-pyrimidinediyl diether (461 mg, 0.523 mmol) and methanesulfonamide (4.97 g, 52.3 mmol). After being stirred for 3 min. at ambient temperature, the slurry is cooled to 0° C., followed by addition of 4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine (10.0 g, 52.3 mmol), prepared as in example 6, in 61 mL of t-butyl alcohol. The thick slurry is vigorously stirred for 48 h at which time TLC (10% EtOAc/hexane for enol ether, and 30% EtOAc/hexane or 10% MeOH/CHCl 3  for the diol product) and  1  H NMR (400 MHz) on an aliquot indicate complete reaction. It is noted that the exo enol ether (4-ethylidene-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine) does not react to a significant extent. 
     The mixture is treated with iodine (133 g, 523 mmol) and calcium carbonate (26.2 g, 261 mmol). After being warmed to ambient temperature the thick slurry is stirred for 48 h. The mixture is cooled to 12° C. and sodium sulfite (70 g) is added in four portions over 20 min with ice cooling. The brown mixture is stirred for 15 min and turns green. Filtration by suction through a pad of Celite 545® (70 g) and extensive washing with CH 2  Cl 2  (1.0 L) gives a two phase mixture. The water phase is extracted with CH 2  Cl 2  (3×800 mL). The combined organic phases are washed with brine (1.2 L), dried over anhydrous Na 2  SO 4  (180 g) and concentrated in vacuo to give 12.0 g of crude product as a yellow oil which does not solidify upon standing at ambient temperature. The oil is chromatographed on 150 g of silica gel. Elution with less than one liter of 2% MeOH in CH 2  Cl 2  yields 4(S)-4-ethyl-4-hydroxy-4,7-dihydro-4,7-dihydro-1H-pyrano[3,4-c]pyridin-3,8-dione as a yellow oil. Chiral HPLC (Chiracel OD, EtOH/hexane 3:97, λ 254 nm) shows a S/R ratio of 39:1. This ratio is confirmed by  1  H NMR (400 MHz) shift study with Eu(hfc) 3 , in which a S/R ratio of 37:1 is demonstrated. 
     EXAMPLE 8 
     4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine 
     A warmed solution of 4-ethylidene-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine, prepared as in example 3 (0.35 g, 1.83 mmol) in 30 mL of n-propanol is purged with a stream of nitrogen for 20 minutes prior to the addition of Wilkinson&#39;s catalyst, tris(triphenylphosphine)rhodium(I) chloride (0.025 g, 0.027 mmol). The reaction mixture is stirred at reflux for 15 hours under nitrogen and again treated with Wilkinson&#39;s catalyst (0.025 g). After 2 hr, the flask is charged with potassium carbonate (0.50 g, 3.60 mmol), and heated for 2 hr. The mixture is then treated with Wilkinson&#39;s catalyst (0.025 g). The mixture is heated to reflux for an additional 2 hr, filtered through Celite 545® and the filtrate is concentrated in vacuo.  1  H NMR analysis shows a 1:8.3 ratio of exo- (4-ethylidene-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine) to endocyclic (4-ethyl-8-methoxy-1H-pyrano[3,4-c] pyridine) product. The dark residue is chromatographed on silica gel using 5% EtOAc/hexanes to provide 4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine, the endocyclic form, as a colorless oil.  1  H-NMR (CDCl 3 , 400 MHz): δ1.13 (t, J=7.4 Hz, 3H, --CH 3 ); 2.30 (q, J=7.4 Hz, 2H, --CH 2  --); 3.95 (s, 3H, --OCH 3 ); 5.04 (s, 2H, --CH 2  O); 6.55 (d, J=5.4 Hz, 1H, Aromatic); 8.04 (d, J=5.4 Hz, Aromatic). Anal. Calcd for C 11  H 13  NO 2 .0.10CHCl 3 .0.05C 3  H 8  O: C, 65.54; H, 6.60; N,6.79. Found: C, 65.48; H, 6.57; N, 6.89. Mass Spectrum (FAB+): m/e 192 (M+1,100%). 
     EXAMPLE 9 
     4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c ]pyridine-cis-3,4-diol 
     A 25-mL round bottom flask, equipped with a magnetic stirring bar, is successively charged with N-methylmorpholine-N-oxide (826 mg, 7.05 mmol), acetone (1.3 mL), water (3.3 mL), t-butyl alcohol (0.6 mL) and 4% wt osmium tetroxide (water solution, 133 mg). The mixture is cooled to 0° C., followed by addition of 4-ethyl-8-methoxy-1H-pyrano[3,4-c]pyridine (1.50 g, 6.65 mmol), prepared as in example 5. The mixture is allowed to warm to ambient temperature and stirred for 20 h. A slurry of 100 mg of Na 2  SO 3 , 800 mg of talc and 4.6 mL of water is added. After being stirred for 10 min, the mixture is filtered through a short pad of Celite 545® and washed with 20 mL of 10% MeOH in CHCl 3 . The filtrate is neutralized with 2N HCl to near pH 7 and diluted with ˜20 mL of brine. The aqueous layer is extracted with 10% MeOH in CHCl 3  (2×15 mL) and the combined organic layers are dried over anhydrous Na 2  SO 4  and concentrated in vacuo to afford crude product. Recrystallization from methanolmethyl t-butyl ether yields 4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-cis-3,4-diol as a crystalline solid.  1  H NMR (400 MHz, CDCl 3 ) δ0.93 (t, J=7.6 Hz, 3H), 1.87 (q, J=7.6 Hz, 2H), 2.63 (s, 1H), 3.45 (d, J=5.1 Hz, 1H),3.98 (s,3H), 4.75 (ABq, J AB  =16.4 Hz, Δv=68.0 Hz, 2H), 5.21 (d, J=5.1 Hz, 1H), 7.14 (d, J=5.2 Hz, 1H), 8.11 (d, J=5.2 Hz, 1H). 
     EXAMPLE 10 
     3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol and acetic acid 3(R)-4(R)-4-ethyl4-hydroxy-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3-yl ester 
     A 10 mL round bottom flask is equipped with a magnetic stirring bar. The flask is charged with racemic 4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]Pyridine-cis-3,4-diol (100 mg, 0.444 mmol), prepared as in example 9 and 100 mg of lipase from Candida cylindracea enzyme (Type VII, Sigma L-1754, lot 38F-0858), followed by addition of 5 mL of vinyl acetate. The suspension is stirred at ambient temperature for 24 h. The mixture is filtered and the solids are washed with 20 mL of methylene chloride. The combined filtrates are concentrated in vacuo. The resultant residue is washed with 30% EtOAc in hexane. Filtration gives a white solid which is a mixture of 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol and acetic acid 3(R)-4(R)-4-ethyl-4-hydroxy-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3-yl ester as indicated by  1  H NMR spectrum. Chromatography on silica gel yields (50%) of 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol and (42%) of acetic acid 3(R)- 4(R)-4-ethyl-4-hydroxy-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3-yl ester. The optical purity (80% ee) of 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol is determined by examination of the chiral HPLC and  1  H NMR chiral shift study on 4(S)-4-ethyl-4-hydroxy-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one derived from 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol. A shift study on acetic acid 3(R)-4(R)-4-ethyl-4-hydroxy-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3-yl ester with Eu(hfc) 3  indicates one enantiomer. 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol: mp 138° C. (dec.). [α]Δ=-52.1° (MeOH, c 1.44).  1  H NMR (CDCl 3 , 400 MHz): δ0.93 (t, J=7.6 Hz, 3H), 1.87 (q, J=7.6 Hz, 2H), 2.63 (s, 1H), 3.45 (d, J=5.1 Hz, 1H), 3.98 (s, 3H), 4.75 (ABq, J AB  =16.4 Hz, Δv=68.0 Hz, 2H), 5.21 (d, J=5.1 Hz, 1H), 7.14 (d, J=5.2 Hz, 1H), 8.11 (d, J=5.2 Hz, 1H). Elemental analysis: Calculated for C 13  H 17  NO 5  : C 58.66, H 6.71, N 6.22. Found: C 58.75, 6.74, N6.17. Acetic acid 3(R)-4(R)-4-ethyl-4-hydroxy-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3-yl ester: mp 141°-143° C. [α]Δ=+92.1° (MeOH, c 1.27).  1  H NMR (CDCl 3 , 400 MHz) δ0.89 (t, J=7.5 Hz, 3H), 1.76 (m,  2H), 2.01 (s, 3H), 2.07 (s, 1H), 3.89 (s, 3H), 4.61 (ABq, J AB  =16.6 Hz, Δv=27.3 Hz, 2H), 6.09 (s, 1H), 7.04 (d, J=5.3 Hz, 1H), 8.03 (d, J=5.3 Hz, 1H). HRMS (EI+), Calculated for C 13  H 17  INO 5  : 267.1104 Found: 267.1107. 
     EXAMPLE 11 
     4(S)-4-ethyl-4-hydroxy-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one 
     A 10-mL round bottom flask is equipped with a magnetic stirring bar. The flask is charged with a mixture of 3(R)-4(S)-4-ethyl-8-methoxy-3,4-dihydro-1H-pyrano[3,4-c]pyridine-3,4-diol (50 mg, 0.222 mmol), prepared as in example 10, calcium carbonate (386 mg, 3.86 mmol) and iodine (1.95 g, 7.68 mmol) in 4 mL of MeOH-H 2  O (10:1). The mixture is stirred at ambient temperature for 40 h, then poured into 10 mL of saturated sodium metabisulfate. The mixture is extracted with 10% MeOH/CH 2  Cl 2  (3×10 mL). The combined extracts are washed with brine (10 mL), dried over anhydrous sodium sulfate and concentrated in vacuo yielding 4(S)-4-ethyl-4-hydroxy-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one as a yellow oil. Chiral HPLC (Chiracel OD 250×16, 254 nm, 3% EtOH/hexane) and  1  H NMR shift study with Eu(hfc) 3  both indicate a ratio of 9:1 (80% ee). [α] D  =+74.0° (CHCl 3 , c 2.50).  1  H NMR (CDCl 3 , 400 MHz) δ0.89 (t, J=7.4 Hz, 3H), 1.72 (q, J=7.4 Hz, 2H), 3.55 (s, 1H), 3.93 (s, 3H), 5.36 (ABq, J AB  =15.5 Hz, Δv=125 Hz, 2H), 7.09 (d, J=5.3 Hz, 1H), 8.14 (d, J=5.3 Hz, 1H). 
     EXAMPLE 12 
     4(S)-4-ethyl-4-hydroxy-6-chloro-4,7-dihydro-1H-pyrano[3,4-c]pyridin-3,8-dione 
     A 50-mL round-bottom flask, equipped with a magnetic stirring bar, a water cooled condenser and an oil bath, is charged with 4(S)-4-ethyl-4-hydroxy-6-chloro-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one (500 mg, 1.94 mmol), prepared as described in example 5, and 20 mL of 6N aqueous HCl. The mixture is brought to reflux for 6 hr. Water is removed in vacuo at 45° C. The solid residue is dissolved in 15 mL of 10% MeOH in CHCl 3  and filtered through a short pad of silica gel eluting with 10% MeOH in CHCl 3  yielding 4(S)-4-ethyl-4-hydroxy-6-chloro-4,7-dihydro-1H-pyrano[3,4-c]pyridin-3,8-dione as a solid after concentration: mp 208°-209° C. [α] D   25  +58.1° (c 1.98,MeOH). (literature for optically pure 4(S)-4-ethyl-4-hydroxy-6-chloro-4,7-dihydro-1H-pyrano[3,4-c]pyridin-3,8 -dione, [α] D   23  +58.5° (c 0.85, MeOH), [α] D   23  +71.9° (c 2.00, MeOH) see Comins, D. L.; Baevsky, M. F.; Hong, H. J. Am. Chem. Soc. 1992, 114, 10971).  1  H NMR (400 MHz, DMSO-d6) δ0.80 (t, J=7.3 Hz, 3H), 1.78 (m, 2H), 5.37 (ABq, JAB=5.6 Hz, Δv=42.6 Hz, 2H), 6.42 (s, 1H), 6.92 (s, 1H), 12.3 (br. s, 1H). c 0.67).  1  H NMR (D 2  O, 400 MHz) δ0.92(t, J=7.2 Hz, 3H), 1.89 (m, 2H), 2.55 (m, 2H), 2.81 (s, 3H), 2.96 (m, 2H), 3.11 (m, 2H), 3.41 (m, 3H), 3.71 (s, 2H), 5.32 (ABq, JAB=16.1 Hz, Δv=54.2 Hz, 2H), 6.84 (s, 1H), 6.97 (s, 1H), 7.00 (s, 1H). Elemental analysis: Calculated for C 28  H 30  N 4  O 6 .2HCl. H 2  O: C 55.18, H 5.62, N 9.19, Cl 11.63. Found: C 55.41, H 5.70, N 9.24, Cl 11.52. 
     EXAMPLE 13 
     7-(4-methylpiperazinomethylene)-10,11-ethylenedioxy-20(S)-camptothecin dihydrochloride 
     A 4-necked 12-L round-bottom flask is equipped with a mechanical stirrer and water-cooled condenser. Under nitrogen, the flask is charged with 1 M boron trichloride in methylene chloride (4 L, 4.00 mol). The solution is cooled to -20° C., then 1,4-benzodioxane-6-amine (500 g, 3.31 mol) available from the Aldrich Chemical Company, Inc., 1001 West Saint Paul Avenue, Milwaukee, Wis. 53233, is added as a solution in methylene chloride (250 mL) over 30 min. The temperature increases to 10° C. during the addition and is subsequently re-cooled back to -10° C. Chloroacetonitrile (250 mL, 3.95 mol) is added over 5 min, followed by addition of aluminum chloride (485 g, 3.64 mol). The resulting dark mixture is heated at reflux for 24 h. The reaction mixture is cooled to ambient temperature and transferred to two 20-L separatory funnels, each containing 10 L of water. After being stirred for 2.5 h, the organic layer is separated and the aqueous layer is extracted with methylene chloride (4×4 L). The combined organic layers are washed with brine (4 L), dried over anhydrous sodium sulfate and concentrated in vacuo. The resultant thick brown slurry is successively treated with 600 mL of methylene chloride and 2 L of hexane. The mixture is stirred at 0° C. for 30 min. The precipitate is collected by filtration on a Buchner funnel, washed with hexane (1 L) and dried in vacuo. at 30° C. yielding 1-(7-amino-2,3-dihydro-benzo[1,4]dioxin-6-yl)-2-chloro-ethanone as a yellow powder. Reverse HPLC (Spherisorb ODS-25 micron, 1:1 MeCN-H 2  O) indicated a purity of 91%. Recrystallization from CH 2  Cl 2  provides an analytical sample as a yellow crystalline solid: mp 130° C. (dec.).  1  H NMR (CDCl 3 , 200 MHz) δ4.24 (m, 2H), 4.32 (m, 2H), 4.59 (s, 2H), 6.21 (s, 1H), 6.23 (br. s, 2H), 7.29 (s, 1H). Elemental analysis: Calculated for C 10  H 10  ClNO 3  : C 52.76, H 4.43, N 6.15. Found: C 52.62, H 4.42, N 6.12. 
     A 4-necked 12-L round-bottom flask is equipped with a mechanical stirrer and 500-mL addition funnel. Under nitrogen, the flask is charged with 1-(7-amino-2,3-dihydro-benzo[1,4]dioxin-6-yl)-2-chloro-ethanone (595 g, 2.61 mol), as prepared above, triethylamine (474 mL, 3.40 mol), and anhydrous acetonitrile (3.5 L). The solution is cooled to 0° C., then methyl malonyl chloride (364 mL, 3.40 mol) is added over 35 min. The cooling bath is removed and the mixture is stirred for 5 h. To the resultant slurry is added 25% sodium methoxide in methanol (596 mL, 2.61 mol) over 10 min. After being stirred at ambient temperature for 2 h, the now very thick yellow slurry is diluted with water (3 L). The precipitate is collected on a Buchner funnel and washed with water (3 L). The yellow solid is dried in vacuo. at 60° C. yielding 9-chloromethyl-7 -oxo-2,3,6,7-tetrahydro[1,4]dioxino[2,3-g]quinoline-8-carboxylic acid methyl ester as a yellow solid. This crude product is used for the next step without further purification. Recrystallization from MeOH-DMSO (1:1) gave an analytical sample: mp&gt;300° C. (dec.).  1  H NMR (DMSO-d6, 400 MHz) δ3.85 (s, 3H), 4.32 (s, 2H), 4.36 (s, 2H), 4.83 (s, 2H), 6.83 (s, 1H), 7.40 (s, 2H), 12.0 (s, 1H). Elemental analysis: Calculated for C 10  H 10  ClNO 3  : C 54.29, H 3.91, N 4.52. Found: C 53.68, H 3.84, N 4.48. HRMS (EI+): Calculated for C 10  H 10  ClNO 3  : 309.0404. Found: 309.0405. 
     A 4-necked 5-L round-bottom flask is equipped with a mechanical stirrer and water-cooled condenser. Under nitrogen, the flask is charged with 9-chloromethyl-7-oxo-2,3,6,7-tetrahydro[1,4 ]dioxino[2,3-g]quinoline-8-carboxylic acid methyl ester (360 g, 1.16 mol), as prepared above, as a suspension in phosphorus oxychloride (1.8 kg). The mixture is heated to reflux generating a black solution. After being heated at reflux for 20 h, the reaction mixture is allowed to cool to ambient temperature and transferred to a 25-L separatory funnel containing 18 L of ice water. After being stirred vigorously for 1.5 h, the precipitate is collected on a Buchner funnel, washed with water (3 L) and dried in vacuo. at 50° C. yielding 7-chloro-9-chloromethyl-2,3-dihydro[1,4]dioxino[2,3-g]quinoline-8-carboxylic acid methyl ester as a dark crystalline solid: mp 130°-132° C.  1  H NMR (CDCl 3 , 200 MHz) δ4.05 (s, 3 H), 4.42 (s, 4H), 4.81 (s, 2H), 7.50 (s, 2H). Elemental analysis: Calculated for C 14  H 11  Cl 2  NO 4  : C 51.24, H 3.38, N 4.27. Found: C 51.10, H 3.34, N 4.33. 
     A 4-necked 5-L round-bottom flask is equipped with a mechanical stirrer and 250-mL addition funnel. Under nitrogen, the flask is charged with 7-chloro-9-chloromethyl-2,3-dihydro[1,4]dioxino[2,3-g]quinoline-8-carboxylic acid methyl ester (340 g, 1.04 mol), as prepared above, and methylene chloride (2 L). To the stirred solution is added N-methylpiperazine (237 mL, 2.14 mol) over 10 min. After the addition is complete, the reaction mixture is stirred at ambient temperature for 15 h. The reaction mixture is poured into water (3 L) and the organic layer is separated. The aqueous layer is extracted with methylene chloride (3×2 L). The combined organic layers are washed with brine (2 L), dried over anhydrous sodium sulfate and concentrated in vacuo. The resultant thick brown slurry is successively treated with methylene chloride (300 mL) and hexane (1.5 L). The mixture is swirled at 0° C. for 30 min. The precipitate is collected on a Buchner funnel, washed with hexane (1 L) and dried in vacuo. at 30° C. yielding 7-chloro-9-chloromethyl-2,3-dihydro[1,4]dioxino[2,3-g]quinoline-8-carboxylic acid methyl ester as a yellow powder: mp 143° C.  1  H NMR (CDCl 3 , 200 MHz) δ2.28 (s, 3H), 2.38-2.49 (m, 8H), 3.81 (s, 2H), 3.96 (s, 3H), 4.59 (s, 2H), 4.40 (s, 4H), 7.45 (s, 1H), 7.65 (s, 1H). Elemental analysis: Calculated for C 19  H 22  ClN 3  O 4  : C 58.24, H 5.66, N 10.72. Found: C 58.08, H 5.72, N 10.63. 
     A 4-necked 5-L round-bottom flask is equipped with an overhead mechanical stirrer, a water-cooled condenser and nitrogen flow is maintained. The flask is charged with 7-chloro-9-chloromethyl-2,3-dihydro[1,4]dioxino[2,3-g]quinoline-8-carboxylic acid methyl ester (194 g, 495 mol), as prepared above, as a solution in 1 L of methylene chloride. To the solution is added 1 M diisobutylaluminum hydride in methylene chloride (2.00 L, 2.00 mol) over 15 min. The solution is heated to reflux during the addition. The reaction is allowed to cool to ambient temperature and stirred for 4 h. The reaction mixture is transferred to a 15-L separatory funnel containing a saturated solution of Rochelle&#39;s salt (5 L). After being stirred for 2.5 h, the organic layer is separated and the aqueous layer is extracted with methylene chloride (3×2.5 L). The combined organic layers are washed with brine (3 L), dried over anhydrous sodium sulfate and concentrated in vacuo. The resultant thick brown slurry is successively treated with methylene chloride (500 mL) and hexane (1 L). The mixture is swirled at 0° C. for 30 min. The precipitate is collected on a Buchner funnel, washed with 1 liter of hexane and dried in vacuo. at 30° C. yielding [7-chloro-9-(4-methyl-piperazin-1-ylmethyl)-2,3-dihydro-[1,4]dioxino[2,3-g]quinolin-8-yl]-methanol as a yellow crystalline solid: mp 178°-180° C.  1  H NMR (CDCl 3 , 200 MHz) δ2.26 (s, 3H), 2.63 (br. s, 4H), 4.00 (s, 2H). 4.39(s, 4H), 4.93 (s, 2H), 6.10 (br. s, 1H), 7.46 (s, 1H), 7.51 (s, 1H). Elemental analysis: Calculated for C 18  H 22  ClN 3  O 3  : C 59.42, H 6.09, N 11.55. Found: C 59.41, H 6.12, N 11.46. 
     A 3-necked 4-L round-bottom flask is equipped with a mechanical stirrer and a 250-mL addition funnel. Under nitrogen, the flask is charged with methylene chloride (800 mL), followed by addition of oxalyl chloride (28.8 mL, 330 mmol). The solution is cooled to -78° C. and dimethyl sulfoxide (46.7 mL, 660 mmol) is added over 6 min. The temperature of the solution increases to -58° C. during the addition and is cooled back to about -70° C. After stirring for 3 min, [7-chloro-9-(4-methyl-piperazin-1-ylmethyl)-2,3-dihydro-[1,4]dioxino[2,3-g]quinolin-8-yl]-methanol (100 g, 275 mmol), as prepared above, is added as a solution in 140 mL of methylene chloride over 10 min. The yellow slurry is stirred for 45 min, then anhydrous triethylamine (153 mL, 1.10 mol) is added over 4 min. Stirring is continued for 10 min at -78° C., then the cooling bath is removed. The reaction mixture is allowed to warm to -5° C. and poured into 2 L of water. The organic layer is separated and the aqueous layer is extracted with methylene chloride (3×1.5 L). The combined organic layers are washed with brine (2 L), dried over anhydrous sodium sulfate and concentrated in vacuo yielding 7-chloro-9-(4-methyl-piperazin-1-ylmethyl)-2,3-dihydro-[1,4 ]dioxino [2,3-g]quinolin-8-carbaldehyde as a dark solid. This crude product is used for the next step without further purification. Recrystallization from CH 2  Cl 2  -MeOH (5:1) provided an analytical sample as a light yellow solid: 140°-142° C. (dec.).  1  H NMR (CDCl 3 , 200 MHz) δ2.30 (s, 3H), 2.45, 2.59 (m, 8H), 3.98 (s, 2H). 4.41(s, 4H), 7.46 (s, 1H), 7.53 (s, 1H), 10.4 (s, 1H). Elemental analysis: Calculated for C 18  H 20  ClN 3  O 3  : C 59.75, H 5.57, N 11.61. Found: C 59.78, H 5.62, N 11.64. 
     A 4-necked 12-L round-bottom flask is equipped with a mechanical stirrer and water-cooled condenser. Under nitrogen, the flask is charged with 7-chloro-9-(4-methyl-piperazin-1-ylmethyl)-2,3-dihydro-[1,4]dioxino[2,3-g]quinolin-8-carbaldehyde (120 g, 346 mmol), as prepared above, sodium iodide (1.04 kg, 6.92 mmol), and 3 L of acetonitrile. To the yellow suspension is added concentrated hydrogen chloride (59.7 mL, 726 mmol) over 5 min. The white slurry is refluxed for 15 hr. The solvent is mostly removed by short-path distillation in vacuo. The resultant thick slurry is cooled to ambient temperature and treated with 2.5 L of water and 2.5 L of methylene chloride. The organic layer is separated and the aqueous layer is extracted with methylene chloride (3×2 L). The combined organic layers are washed with brine (2.5 L), dried over anhydrous sodium sulfate, concentrated and dried in vacuo at 30° C. yielding 7-iodo-9-(4-methyl-piperazin-1-ylmethyl)- 2,3-dihydro-[1,4]dioxino[2,3-g]quinolin-8-carbaldehyde as a yellow solid. This crude product is used for the next step without further purification. Recrystallization from CH 2  Cl 2  -MeOH (1:1) gave an analytical sample as an off-white solid: 198°-200° C. (dec.).  1  H NMR (CDCl 3 , 200 MHz) δ2.30 (s, 3H), 2.43, 2.57 (m, 8H), 3.93 (s, 2H). 4.40(s, 4H), 7.47 (s, 1H), 7.50 (s, 1H), 10.1 (s, 1H). Elemental analysis: Calculated for C 18  H 20  IN 3  O 3  : C 47.70, H 4.45, N 9.27. Found: C 47.78, H 4.45, N 9.26. 
     A 3-necked 2-L round-bottom flask is equipped with an overhead mechanical stirrer and nitrogen flow is maintained. The flask is charged with 7-chloro-9-(4-methyl-1-piperazin-1-ylmethyl)-2,3-dihydro-[1,4 ]dioxino [2,3-g]quinolin-8-carbaldehyde (105 g, 232 mmol), as prepared above, as a suspension in 700 mL of methanol. The mixture is cooled to 0° C., then sodium borohydride (8.76 mmol) is added in three portions over 15 min. The mixture is allowed to warm to ambient temperature and stirred for 2 hr. The solvent is mostly removed in vacuo and the resultant residue is treated with 2.5 L of water and extracted with methylene chloride (4×1.5 L). The combined organic layers are washed with brine (2.5 L), dried over anhydrous sodium sulfate, filtered, and concentrated in vacuo. The resultant solid residue is successively treated with 300 mL of methylene chloride and 300 mL of MeOH-EtOAc (1:1). The mixture was swirled at 0°  C. for 30 min. The precipitate is filtered by suction, washed with 500 mL of hexane and dried in vacuo at 30° C. yielding [7-iodo-9-(4-methyl-piperazin-1-ylmethyl)-2,3-dihydro-[1,4]dioxino[2,3-g]quinolin-8-yl]-methanol as an off-white powder: mp 201°-203° C. (dec.).  1  H NMR (CDCl 3 , 200 MHz): δ2.26 (s, 3H), 2.62 (m, 8H), 4.02 (s, 2H). 4.39 (s, 4H), 4.93 (m, 2H), 6.05 (br. s, 1H), 7.49 (s, 1H), 7.51 (s, 1H). HRMS (EI+): Caculated for C 18  H 22  N 3  O 3  : 455.0706. Found: 455.0699. 
     A 1-liter 3-necked round-bottom flask is equipped with an overhead mechanical stirrer and a condenser, under nitrogen, the flask is charged with 1N hydrochloric acid (600 mL) and 101 g of crude 4(S)-4-ethyl-4-hydroxy-8-methoxy-1,4-dihydro-pyrano[3,4-c]pyridin-3-one prepared as in example 3. The resulting solution is heated at reflux for 14 hr. The reaction mixture is cooled to ambient temperature and then concentrated to a solid. The solids are recrystallized in methanol (75 mL) yielding 4(S)-4-ethyl-4-hydroxy-4,7-dihydron-1H-pyrano[3,4-c]pyridin-3,8-dione. mp 224°-226° C.;  1  H-NMR (400 MHz, DMSO-d6) δ11.85 (s, 1H), 7.44 (d, J=7 Hz, 1H), 6.36 (d, J=7 Hz, 1 n), 6.28 (s, 1H), 5.24 (s, 2H), 3.36 (s, 3H), 2.99 (s, 1H), 1.75 (m, 2H), 0.81 (t, J=7 Hz, 3H); Calculated for C10H11NO4; C 57.4%, H 5.30%, N 6.70%: Found; C 56.59%, H 5.26%, N 6.66%; MS (EI) 209 (M+); [a]D 22  +115.4° [c 0.877, MeOH]; Optical purity determined by chiral HPLC: 10% ethanol/hexane, 26° C., 1 mL/min., 1=300 nm, Chiralcel-OD column 250×4.6 mm. i.d. 
     A 4-necked 2-L round-bottom flask is equipped with mechanical stirrer, and water cooled condenser. Under nitrogen, the flask is charged with 7-oxopyrido[5,4-c]-2-oxo-α-3-ethyl-β-3-hydroxy-3,6-dihydropyran (26.7 g, 128 mmol), as prepared above, [7-iodo-9-(4-methyl-piperazin-1-ylmethyl)-2,3-dihydro-[1,4]dioxino[2,3-g]quinolin-8-yl]-methanol (58.1 g, 128 mmol), as prepared above, triphenylphosphine (33.5 g, 128 mmol) and 318 mL of methylene chloride. The suspension is stirred for 15 min, then diethyl azodicarboxylate (20.1 mL, 128 mmol) is added dropwise over 15 min. During the addition, gentle reflux of the solvent is observed. The brown solution is allowed to cool to ambient temperature and stirred for 6.5 h. The solvent is removed in vacuo. and the resulting residue is treated with 400 mL of benzene and swirled for 3 min. The formed precipitate is filtered by suction and washed with 50 mL of cold benzene. The filtrate is concentrated and the resultant solid is chromatographed on silica gel. Elution with 3-50% MeOH in CHCl 3  affords a light yellow solid, which is dissolved in 500 mL MeOH/CH 2  Cl 2  (1:100). Recrystallization is then initiated by addition of ethyl acetate and filtration by suction and drying in vacuo. The filtrate from the recrystallization is partially concentrated and a second recrystallization is performed. A third recrystallization in the same fashion yields 4(S)-4-ethyl-4-hydroxy-7-[7-iodo-9-(4-methyl-piperazin-1-ylmethyl)-2,3-dihydro-[1,4]dioxino[2,3-g]quinolin-8-ylmethyl]-4,7-dihydro-1H-pyrano[3,4-c]pyridine-3,8-dione slightly contaminated with ethyl acetate: mp 158°-163° C. (dec.). [α] D  =+1.9° (MeOH, c 1.29).  1  H NMR (CDCl 3 , 200 MHz) δ0.98 (t, J=7.3 Hz, 3H), 1.83 (q, J=7.3 Hz, 2H), 2.19 (s, 3H), 2.15-2.49 (m, 9H), 3.54 (m, 2H), 4.42 (m, 4H), 5.46 (ABq, J AB  =15.4 Hz, Δv=94.4 Hz, 2H), 5.51 (ABq, J AB  =16.3 Hz, Δv=110, Hz, 2H), 6.48 (d, J=7.2 Hz, 1H), 7.20 (d, J=7.2 Hz, 1H), 7.38 (s, 1H), 7.40 (s, 1H). HRMS (EI+): Calculated for C 28  H 31  IN 4  O 6  : 646.1239 Found: 646.1304. 
     A 4-necked 3-L round-bottom flask is equipped with mechanical stirrer, and water-cooled condenser. Under nitrogen, the flask is charged with 4(S)-4-ethyl-4-hydroxy-7-[7-iodo-9-(4-methyl-piperazin-1-ylmethyl)-2,3-dihydro-[1,4]dioxino[2,3-g]quinolin-8-ylmethyl]-4,7-dihydro-1H-pyrano[3,4-c]pyridine-3,8-dione (30.0 g, 46.4 mmol), as prepared above, palladium(II) acetate (213 mg, 0.928 mmol), anhydrous potassium carbonate powder (12.8 g, 92.8 mmol), triphenylphosphine (6.09 g, 23.2 mmol) and anhydrous acetonitrile (1.8 L). The suspension is brought to reflux during which time the solids dissolve. As the reflux is continued for 16 h, the product precipitates. The reaction mixture is cooled to 0° C. and stirred for an additional 2.5 h. The precipitate is collected on a Fritted funnel and the resulting yellow cake is treated with 1 L of chloroform. The suspension is filtered and washed with chloform (5×200 mL), the combined flitrates are concentrated to 300 mL and treated with 30.0 g of triphenylphosphine. After being stirred at ambient temperature for 30 min, the solution is treated with 100 mL of acetone. The resulting precipitate is filtered by suction yields 7-(4-methylpiperazinomethylene)-10,11-ethylenedioxy-20(S)-camptothecin as a light yellow powder. The product is further purified by stirring as a solution in 220 mL of MeOH/CHCl 3  (1:10) containing 5.6 g of triphenylphosphine, followed by precipitation with 50 mL of acetone. The precipitate is collected on a Buchner funnel and dried in vacuo. At this stage, analysis shows palladium and phosphorus. Further purification is carried out by dissolving the compound in 165 mL of MeOH/CHCl 3  (1:10), followed by precipitation with 150 mL of acetone; filtration and drying in vacuo at ambient temperature. Analysis indicated nondetectable amount (&lt;2 ppm) of palladium and phosphorus in the product: mp: 275° C. (dec.). [α] D  =+22.6 ° (CHCl 3 , c 1.02).  1  H NMR (CDCl 3 , 400 MHz): δ1.04 (t, J=7.4 Hz, 3H), 1.87 (m, 2H), 2.31 (s, 3H), 2.20-2.59 (m, 9H), 3.97 (s, 2H), 4.46 (s, 4H), 5.32 (s, 2H), 5.55 (ABq, J AB  =16.2 Hz, Δv=180 Hz, 2H), 7.60 (s, 1H), 7.66 (s, 1H), 7.72 (s, 1H). Elemental analysis: Calculated for C 28  H 30  N 4  O 6  : C 64.85, H 5.83, N 10.80. Found: C 64.34, H 5.83, N 10.71. 
     A four-necked 1-L round-bottom flask is equipped with a mechanical stirrer and water-cooled condenser. Under nitrogen, the flask is charged with free base 7(4-methylpiperazinomethylene)-10,11-ethylenedioxy-20(S)-camptothecin (14.0 g, 27.0 mmol), as prepared above, and 350 mL of 6N HCl. The suspension is brought to reflux for 35 min. A small amount of precipitate is generated after initial complete solution formation. Without being cooled, the mixture is filtered through a Supor (0.45 m) filtering membrane. Washing with hot 6N HCl (100 mL) dissolves the above precipitate. The combined flitrates are cooled to 35° C. Recrystallization is initiated by addition of 20 mL of 200 proof ethanol. After stirring for 1 h, 150 mL more ethanol is added. The mixture is allowed to stand at 0° C. for 24 h. Filtration and drying in vacuo. at 70° C. yields 7-(4-methylpiperazinomethylene)-10,11-ethylenedioxy-20(S)-camptothecin dihydrochloride as a yellow powder. A  1  H NMR shift study with Eu(hfc) 3  indicates a single enantiomer. mp 280° C. (dec.). [α] D  =-6.72° (H 2  O, c 0.67).  1  H NMR (D 2  O, 400 MHz) δ0.92 (t, J=7.2 Hz, 3H), 1.89 (m, 2H), 2.55 (m, 2H), 2.81 (s, 3H), 2.96 (m, 2H), 3.11 (m, 2H), 3.41 (m, 3H), 3.71 (s, 2H), 5.32 (ABq, J AB  =16.1 Hz, Δv=54.2 Hz, 2H), 6.84 (s, 1H), 6.97 (s, 1H), 7.00 (s, 1H). Elemental analysis: Calculated for C 28  H 30  N 4  O 6 . 2HCl. H 2  O: C 55.18, H 5.62, N 9.19, Cl 11.63. Found: C55.41, H 5.70, N 9.24, Cl 11.52.