Abstract:
Novel quinazolinone compounds of the formula: ##STR1## wherein R 5 , R 6 , R 7 , R 8 , R and X are defined in the specification, which have angiotensin II (AII) antagonizing activity, intermediates useful in the preparation of the compounds, methods of producing and using the compounds to alleviate angiotensin induced hypertension and treat congestive heart failure in mammals.

Description:
This is a continuation-in-part of copending application Ser. No. 07/648,492 filed on Jan. 30, 1991 now abandoned. 
    
    
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     This invention relates to certain novel 2, 3, 6 substituted quinazolinone compounds which have demonstrated enhanced in vivo activity as angiotensin II (AII) antagonists and are therefore useful in alleviating angiotensin induced hypertension and for treating congestive heart failure. 
     2. Description of the Prior Art 
     The enzyme renin acts on a blood plasma α 2  -globulin, angiotensinogen, to produce angiotensin I, which is then converted by angiotensin converting enzyme to AII. The substance AII is a powerful vasopressor agent which is implicated as a causative agent for producing high blood pressure in mammals. Therefore, compounds which inhibit the action of the hormone angiotensin II (AII) are useful in alleviating angiotensin induced hypertension. 
     Furukawa et al., in U.S. Pat. No. 4,340,598, issued Jul. 20, 1982, discloses hypotensive and angiotensin II receptor blocking imidazole derivatives of the formula: ##STR2## wherein R 1  is lower alkyl or phenyl C 1-2  alkyl optionally substituted with halogen or nitro; R 2  is lower alkyl, cycloalkyl or phenyl optionally substituted; one of R 3  and R 4  is --(CH 2 ) n  COR 5  where R 5  is amino, lower alkoxyl or hydroxyl and n is 0, 1, 2 and the other of R 3  and R 4  is hydrogen or halogen; provided that R 1  is lower alkyl or phenethyl when R 3  is hydrogen, n=1 and R 5  is lower alkoxyl or hydroxyl; and salts thereof. 
     Furukawa et al., in European Patent Application No. 103,647 discloses 4-chloro-2-phenylimidazole-5-acetic acid derivatives useful for treating edema and hypertension and have angiotensin II receptor blocking activity of the formula: ##STR3## where R represents lower alkyl and salts thereof. 
     D. J. Carini, et al. in published European Patent Applications No. 87109919.8, filed Sep. 7, 1987 and No. 89100144.8, filed May 1, 1989 disclose angiotensin II receptor blocking imidazoles of the formula: ##STR4## wherein the definitions of the substituents may be found within the applications. 
     P. Aldrich et al., in U.S. Pat. No. 4,874,867, issued Oct. 17, 1989, describes tetrazole intermediates of the formula: ##STR5## wherein X 2  and X 1  are defined therein. These intermediates are described as useful for producing compounds which are useful as inhibitors of the hormone angiotensin II (AII). 
     D. J. Carini et al., in U.S. Pat. No. 4,880,804, issued Nov. 14, 1989, described substituted benzimidazoles useful as inhibitors of the hormone angiotensin II (AII) of the formula: ##STR6## wherein R 1  is --CO 2  H, --NHSO 2  CF 3 , or ##STR7## R 2  is H, halogen, NO 2 , methoxy, or alkyl of 1 to 4 carbon atoms; R 3  is alkyl of 1 to 6 carbon atoms, alkenyl or alkynyl of 3 to 6 carbon atoms both of which may be optionally substituted with a halogen atom, --OR 4  or up to two --CO 2  R 4  ; with the proviso that when R 3  is methyl, it must be substituted with --OR 4  or --CO 2  R 4  ; R 4  is H, or alkyl of 1-4 carbon atoms; A is H, alkyl of 1 to 10 carbon atoms, C r  F 2r+1  where r=1-6, C 6  F 5 , halogen, alkoxy of 1 to 6 carbon atoms; ##STR8## B is H, alkyl of 1 to 10 carbon atoms, C r  F 2r+1  where r=1-6, C 6  F 5 , halogen or alkoxy of 1 to 6 carbon atoms; X is a carbon-carbon single bond, --CO--, --O--, --NHCO--, or --OCH 2  --. 
     D. J. Carini et al. in published European Patent Application No. 89100142.2, filed May 1, 1989, discloses angiotensin II receptor blocking pyrroles, pyrazoles and triazoles such as: ##STR9## 
     There has been no teaching or suggestion that the heretofore known antagonists of AII have the quinazolinone structure. 
     SUMMARY OF THE INVENTION 
     According to the present invention, there are provided novel compounds of Formula I which have enhanced in vivo angiotensin II-antagonizing properties and are useful as antihypertensives: ##STR10## wherein: R is ##STR11## X is straight or branched alkyl of 3 to 5 carbon atoms; n is 1 to 3; R 5  is H; 
     R 6  is ##STR12## where R 9  is H, straight chain lower alkyl of 1 to 4 carbon atoms, phenyl, substituted phenyl (substitution selection from mono-lower alkyl of 1 to 3 carbon atoms, --CF 3 , nitro, O-alkyl of 1 to 3 carbon atoms, NH 2 ), pyridine, thiophene, or furan; 
     R 10  is H, straight chain lower alkyl of 1 to 4 carbon atoms, phenyl, substituted phenyl (substitution selection from mono-lower alkyl of 1 to 3 carbon atoms, --CF 3 , nitro, O-alkyl of 1 to 3 carbon atoms, NH 2 ), pyridine, thiophene, or furan; 
     provided, however, that R 9  and R 10  cannot both be H, 
     R 11  is H, straight chain or branched lower alkyl of 1 to 4 carbon atoms; 
     R 12  is straight chain lower alkyl of 1 to 4 carbon atoms, phenyl, substituted phenyl (substitution selection from mono-lower alkyl of 1 to 3 carbon atoms, --CF 3 , nitro, O-alkyl of 1 to 3 carbon atoms, NH 2 ), pyridine, thiophene, or furan; 
     R 17  is straight or branched lower alkyl of 1 to 4 carbon atoms; 
     R 7  and R 8  are H; and pharmaceutically acceptable salts of these compounds. 
     The present invention also provides novel intermediate compounds, methods for making the novel 2, 3, 6 substituted quinazolinone angiotensin II antagonizing compounds, methods for making the novel intermediates, methods of using the novel quinazolinone angiotensin II antagonizing compounds to treat hypertension, congestive heart failure and to antagonize the effects of angiotensin II. 
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS 
     FIG. 1 depicts data showing antagonism of the vasopressor response of Antiotensin II in spontaneously hypertensive rats. 
     FIGS. 2-7 depict mean average blood pressure response data in aorta-coarcted hypertensive rats. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     The novel compounds of the present invention are prepared according to the following reaction schemes. 
     Referring to Method A, the quinazolinone intermediates of Formula 5, are prepared from the corresponding substituted anthranilic acids 2 wherein the substituents R 5 , R 6 , R 7  and R 8  are described hereinabove except R 6  may not be: ##STR13## The corresponding anthranilic acid 2 is heated to reflux in alkyl acid anhydride 3 wherein X is alkyl of 3 to 5 carbon atoms to provide the 4H-3,1-benzoxazin-4-ones 4 which are isolated by concentrating the reaction mixtures and used without further purification. When the 4H-3,1-benzoxazin-4-ones 4 are refluxed in ethyl alcohol containing ammonia, or ammonium hydroxide solution, the quinazolinone intermediates 5 are obtained. To prepare compounds for which R 6 , has been excluded from this method, refer to Schemes I to X herein. ##STR14## 
     Referring to Method B, the method of B. Baker, et al., J. Org. Chem. 17, 157 (1952) is used to convert the appropriate substituted aniline 6 into quinazolinone 5 wherein the substituents R 5 , R 6 , R 7  and R 8  are described hereinabove except they may not be as follows: ##STR15## The substituted aniline 6 is reacted with chloral and hydroxylamine hydrochloride to afford an oxime 7 which is cyclized to the isatin 8 in the presence of sulfuric acid. The isatin 8 is then hydrolyzed to the anthranilic acid 9 using 30% aqueous hydrogen peroxide and aqueous sodium hydroxide. Further reaction as in Method A yields the quinazolinone intermediate 5. To prepare compounds for which R 6 , has been excluded from this method, refer to Schemes I to X herein. ##STR16## 
     A general reference to the synthesis of 3,4-dihydro-4-oxo-quinazolines 5 is given in &#34;The Chemistry of Heterocyclic Compounds, Fused Pyrimidines. Part I: Quinazolines&#34;, W. L. F. Armarego; Interscience Publishers (1967), pp. 74-94. Additional references are described in &#34;Heterocyclic Compounds&#34;, Vol. 6, p. 334, R. C. Elderfield (Editor), Wiley and Sons, 1957. 
     Quinazolinone intermediates 5 are then modified according to the following schemes to obtain the novel 2, 3, 6 substituted quinazolinone Angiotensin II antagonizing compounds of the present invention. 
     In Scheme I, 6-methylquinazolinone 10, as prepared by method A, is brominated with N-bromosuccinimide to give the bromomethyl compound 11. Hydrolysis of the bromide with aqueous potassium carbonate in dimethylsulfoxide yields the primary alcohol 12. 
     The alcohol 12 is oxidized with pyridinium dichromate in N,N-dimethylformamide to afford aldehyde 13. The aldehyde 13 is reacted with a variety of Grignard Reagents R 12  MgBr or lithium reagents R 12  Li in tetrahydrofuran wherein R 12  is selected from straight or branched alkyl of 1 to 4 carbon atoms, phenyl, substituted phenyl, pyridyl, thiophene and furan, to give the desired secondary alcohol 14. ##STR17## 
     In an alternate route to 13, as shown in Scheme II, 2-alkylsubstituted-6-iodo-4(1H)-quinazolinone 15, prepared by method A is reacted via a palladium catalyzed carbonylation to give aldehyde 13. 
     Ester 16 is formed by palladium (II) catalyzed coupling of 2-alkylsubstituted-6-iodo-4(1H)-quinazolinone 15 with carbon monoxide and methanol. Further derivatization of 16 with an excess of Grignard R 12  MgX or R 12  Li affords tertiary alcohol 17 where R 12  is hereinbefore defined. ##STR18## 
     The synthetic pathway to tertiary alcohol substituted quinazolinones is shown in Scheme III. ##STR19## 
     As shown in Scheme III, the palladium (II) catalyzed coupling of (trimethylsilyl)acetylene with 2-alkylsubstituted-6-iodo-4(1H)-quinazolinone 15 yields the acetylenic quinazolinone 21. Desilylation of the acetylene with sodium hydroxide in water-methanol gives the terminal acetylene 22. Hydration with catalytic mercuric sulfate-sulfuric acid in acetic acid affords methyl ketone 23. Reduction of ketone 23 with sodium borohydride in ethanol gives secondary alcohol 24. Alternatively, methyl ketone 23 is reacted with Grignard reagents R 12  MgBr or lithium reagent R 12  Li where R 12  is as defined before to yield tertiary alcohols 25. The palladium (II) catalyzed coupling of substituted acetylenes where R 17  is defined as straight or branched lower alkyl of 1 to 4 carbon atoms with 2-alkylsubstituted-6-iodo-4(1H)-quinazolinone 15 yields the acetylenic quinazolinone 26. Hydration of 26 with catalytic mercuric sulfate-sulfuric acid in acetic acid gives ketone 27. Reduction of ketone 27 with sodium borohydride in ethanol gives secondary alcohol 28. Reaction of ketone 27 with Grignard reagent R 12  MgBr or lithium reagent R 12  Li where R 12  is as defined before yields alcohol 29. 
     Carboxylic acid 77, prepared by Method A, as presented in Scheme IV, is converted to the ethyl ester 78 by reaction with ethyl alcohol containing a catalytic amount of sulfuric acid. Reduction of 78 with lithium aluminum hydride in tetrahydrofuran affords alcohol 79. Alcohol 79 is oxidized with pyridinium dichromate to yield aldehyde 80. ##STR20## 
     Reaction of aldehyde 80 with Grignard reagent R 12  MgBr or lithium reagent R 12  Li, where R 12  is hereinbefore defined, affords alcohol 81. Also, ethyl ester 78 is reacted with Grignard reagent R 12  MgBr or lithium reagent R 12  Li to give alcohol 82. 
     The coupling of a quinazolinone intermediate 5 to a biphenyl tetrazole 84 where R is as defined before, which are prepared by the methods of P. E. Aldrich et al., U.S. Pat. No. 4,874,867, issued Oct. 17, 1989, is illustrated in Scheme V. ##STR21## 
     The quinazolinone 5 and the biphenyl 84 are dissolved in acetone or another suitable solvent such as N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidinone, methanol, ethanol, t-butanol, tetrahydrofuran, dioxane or dimethylsulfoxide, in the presence of excess potassium carbonate or another suitable base such as sodium carbonate, cesium carbonate, sodium hydride, potassium hydride, sodium methoxide, sodium ethoxide, sodium t-butoxide or potassium t-butoxide for 2-24 hours, at 20°-60°. The obtained alkylated quinazolinones 85 may be purified by chromatography or used as is in further transformations and/or deprotection. 
     In those cases where the R in the alkylated quinazolinones 85 is a trityl tetrazole, deprotection of the trityl group, as outlined in Scheme VI, is accomplished by refluxing an aqueous acetone solution of the alkylated quinazolinone 86 with a catalytic amount of hydrochloric acid or other suitable acid such as sulfuric, trifluoroacetic or hydrogen chloride for 2-24 hours. The resulting tetrazoles 87 are isolated by flash chromatography or by trituration with ether and collection by filtration. ##STR22## 
     As shown in Scheme VII, alcohol 93 is reacted with an alkylating agent R 17  I, wherein R 17  is alkyl of 1 to 4 carbon atoms, n is 0 to 3 and I is iodide, in the presence of a base, such as sodium hydride, to afford ethers 98. The intermediate ethers 98 are deblocked via dilute acid in acetone to give tetrazoles 99. ##STR23## 
     Alcohol 100, as shown in Scheme VIII, is prepared from 93 by dilute acid hydrolysis to remove the trityl protecting group, is reacted with the desired acid anhydride, (R 17  CO) 2  O wherein R 17  is defined as straight or branched lower alkyl of 1 to 4 carbon atoms or an acid chloride R 17  COCl where n is 0 to 3 in the presence of a base such as pyridine to afford ester 101. 
     Alcohol 93, is prepared via methods of Schemes I, II and XV. 
     An alternate synthetic method of preparing 87 from 5 is illustrated in Scheme IX. ##STR24## 
     Quinazolinone 5 is alkylated in a solvent such as acetone in the presence of potassium carbonate with ##STR25## wherein R 40  is selected from I, Br or --OSO 2  CF 3  and B is selected from appropriate leaving groups such as I, Br, Cl, --OMs, --OTs or --OSO 2  CF 3  to give 108. Palladium or nickel catalyzed coupling of 108 with 109 where M can be --MgBr, --Sn(lower alkyl of 1 to 4 carbon atoms or phenyl), Li or --Zn complex, affords 110 which is deprotected to give 87. 
     Scheme X illustrates the method of preparing 109. Reaction of o-bromobenzonitrile with tri-n-butyltin azide affords 111. Further reaction of 111 with hydrogen chloride and trityl chloride gives 112. Reaction of 112 with a metal M such as magnesium, or n-BuLi or s-BuLi followed by ZnCl 2  or (Me) 3  SnCl affords 109. ##STR26## 
     It will be appreciated that the chemical manipulations of R 5 , R 6 , R 7  and R 8  as outlined in Schemes I-X can be accomplished after alkylation as outlined in Scheme V. Additionally, it will also be appreciated that the chemical manipulations of R 5 , R 6 , R 7  and R 8  as outlined in Schemes VII-VIII can be accomplished before alkylation as outlined in Scheme V. The reactions are performed in a solvent appropriate to the reagents and materials em-ployed and suitable for the transformation being effected. It is understood by those skilled in the art of organic synthesis that the various functionalities present on the molecule must be consistent with the chemical transformations proposed. This will frequently necessitate judgement as to the order of synthetic steps, protecting groups, if required, and deprotection conditions. Substituents on the starting materials may be incompatible with some of the reaction conditions. Such restrictions to the substituents which are compatible with the reaction conditions will be apparent to one skilled in the art. 
     Pharmaceutically suitable salts include both the metallic (inorganic) salts and organic salts; a list of which is given in Remington&#39;s Pharmaceutical Sciences, 17th Edition, pg. 1418 (1985). It is well known to one skilled in the art that an appropriate salt form is chosen based on physical and chemical stability, flowability, hygroscopicity and solubility. Preferred salts of this invention for the reasons cited above include potassium, sodium, calcium, magnesium and ammonium salts. 
     Some of the compounds of the hereinbefore described Schemes have centers of asymmetry. The compounds may, therefore, exist in at least two and often four stereoisomeric forms. The present invention encompasses all stereoisomers of the compounds whether free from other stereoisomers or admixed with other stereoisomers in any proportion and thus includes, for instance, racemic mixture of enantiomers as well as the diastereomeric mixture of isomers. The absolute configuration of any compound may be determined by conventional X-ray cyrstallography. 
     While the invention has been illustrated using the trityl protecting group on the tetrazole, it will be apparent to those skilled in the art that other nitrogen protecting groups may be utilized. Contemplated equivalent protecting groups include, benzyl, p-nitrobenzyl, propionitrile or any other protecting group suitable for protecting the tetrazole nitrogen. Additionally, it will be apparent to those skilled in the art that removal of the various nitrogen protecting groups, other than trityl, may require methods other than dilute acid. 
     The compounds of this invention and their preparation can be understood further by the following examples, but should not constitute a limitation thereof. 
     EXAMPLE 1 
     2-Butyl-6-(methyl)-4(1H)-quinazolinone 
     Method A 
     To 20.0 g of 2-amino-5-methylbenzoic acid is added 60 ml of valeric anhydride. The mixture is heated at reflux for 18 hours and then concentrated under reduced pressure. The resulting brown solid residue is dissolved in a mixture of 200 ml of 30% of ammonium hydroxide solution and 300 ml of ethyl alcohol. This mixture is heated at reflux for 5 hours and then allowed to cool to room temperature. After cooling, the precipitate is collected by filtration. The cake is washed with ethanol and water, then dried under vacuum to give 8.92 g of the quinazolone as a white solid. 
     Method B: 
     The procedure described by B. Baker et. al., J. Org. Chem. 17 157(1952) and Sandmeyer, Helv. Chim. Acta 2, 234(1919) is used. 
     Examples 2-34 in Table 1 are prepared by using the appropriately substituted anthranilic acids by using synthetic method A or B described hereinabove. 
     
                       TABLE I______________________________________ ##STR27##Ex.                                 SynthesisNo.  R.sup.5      R.sup.6 R.sup.7                  R.sup.8                      X        Method MP °C.______________________________________2    H     Br      H   H   (CH.sub.2).sub.3 CH.sub.3                               A      1113    H     H       H   H   (CH.sub.2).sub.3 CH.sub.3                               A      1294    H     Cl      H   H   (CH.sub.2).sub.3 CH.sub.3                               A      1945    H     I       H   H   (CH.sub.2).sub.3 CH.sub.3                               A      257-2586    H     I       H   I   (CH.sub.2).sub.3 CH.sub.3                               A      267-2687    H     CH.sub.3              H   H   (CH.sub.2).sub.3 CH.sub.3                               A      231-2328    H     Cl      H   H   (CH.sub.2).sub.3 CH.sub.3                               A      255-2569    H     H       H   H   (CH.sub. 2).sub.3 CH.sub.3                               A      185-18710   H     CH.sub.3              H   H   (CH.sub.2).sub.3 CH.sub.3                               A      *11   H     OCH.sub.3              H   H   (CH.sub.2).sub.3 CH.sub.3                               B      181-182° C.12   H     Cl      H   H   (CH.sub.2).sub.3 CH.sub.3                               A      194° C.______________________________________ *CI MASS SPEC MH.sup.+  217 
    
     EXAMPLE 13 
     2-Butyl-7-carboethoxy-4(1H)-quinazolinone 
     A mixture of 5.0 g of 2-butyl-7-carboxy-4(1H)-quinazolinone in 100 ml of absolute ethanol containing 2 ml of sulfuric acid is refluxed for 48 hours. The solvent is evaporated in vacuo and the residue partitioned between water and chloroform. The organic layer is washed with aqueous saturated sodium bicarbonate, dried with anhydrous sodium sulfate, filtered and evaporated to a residue which crystallizes from ethyl acetate-hexane to afford 4.5 g of the desired product, mp 145° C. 
     EXAMPLE 14 
     2-Butyl-6-(bromomethyl)-4(1H)-quinazolinone 
     To a suspension of 3.50 g of 6-methylquinazolone in 100 ml of chloroform is added 3.39 g of N-bromosuccinimide and 0.25 g of benzoyl peroxide. The reaction mixture is heated at reflux for 18 hours and then filtered hot. A precipitate of 2.21 g of an inseparable mixture of the desired bromide and starting 6-methyl quinazolinone is obtained and used in the next step without further purification. 
     EXAMPLE 15 
     2-Butyl-6-(hydroxymethyl)-4(1H)-quinazolinone 
     To a suspension of 2.0 g of impure 2-butyl-6-(bromomethyl)-4(1H)-quinazolinone in 35 ml of dimethylsulfoxide and 20 ml of water is added 1.0 g of potassium carbonate. The reaction mixture is heated at reflux for 6 hours, resulting in a complete solution. Upon cooling slowly to room temperature a white precipitate forms and is collected by filtration. The filter cake is purified by flash chromatography on silica gel, eluting with 9:1 chloroform-methanol to give 0.67 g of the desired product as a white solid. CI MASS SPEC 233(M+H). 
     EXAMPLE 16 
     2-Butyl-1,4-dihydro-4-oxo-6-quinazoline-carboxaldehyde 
     To a solution of 0.3 g of 2-butyl-6-(hydroxymethyl)-4(1H)-quinazolinone in 3.5 ml of dry N,N-dimethylformamide is added 1.7 g of pyridinium dichromate. The reaction mixture is stirred at room temperature for 16 hours and then poured into 125 ml of water. The resulting precipitate is removed by filtration and the filtrate extracted with 9:1 chloroform-methanol. The combined organic extracts are dried over magnesium sulfate, filtered and concentrated in vacuo and combined with the precipitate above. The combined solids are purified by flash chromatography on silica gel by eluting with 1:1 ethyl acetate-hexanes to give 0.27 g of the desired product. CI MASS SPEC 231(M+H). 
     EXAMPLE 17 
     2-Butyl-6-(1-hydroxyethyl)-4(1H)-quinazolinone 
     To a solution of 0.60 g of 2-butyl-1,4-dihydro-4-oxo-6-quinazoline-carboxaldehyde in 30 ml of dry tetrahydrofuran, cooled to 0° C. is added dropwise, 2.61 ml of a 3.0M solution of methylmagnesium bromide in diethyl ether. The reaction is stirred at 0° C. for 30 minutes and then quenched with 10 ml of aqueous ammonium chloride. After diluting with 10 ml of water, the reaction mixture is extracted with 9:1 chloroform-methanol. The combined extracts are dried with magnesium sulfate, filtered and concentrated to yield 0.64 g of the desired product. CI MASS SPEC 247(MH + ). 
     EXAMPLE 18 
     2-Butyl-6-(1-hydroxypropyl)-4(1H)-quinazolinone 
     To a solution of 0.25 g of 2-butyl-1,4-dihydro-4-oxo-6-quinazoline-carboxaldehyde in 10 ml of dry tetrahydrofuran, cooled to 0° C., is added 1.63 ml of 2.0M ethyl magnesium bromide in tetrahydrofuran. The reaction mixture is stirred for 30 minutes at 0° C. and quenched with 20 ml of saturated ammonium chloride solution and 20 ml of water. The reaction mixture is extracted with 9:1 chloroform-methanol, dried over magnesium sulfate, filtered and evaporated in vacuo to give 0.26 g of the desired product. CI MASS SPEC 261(MH + ). 
     EXAMPLE 19 
     2-Butyl-1,4dihydro-4-oxo-6-quinazoline-carboxaldehyde 
     To a solution of 1.0 g of 2-butyl-1,4-dihydro-4-oxo-6-iodo-quinazoline and 0.355 g of tetrakis(triphenylphosphine)palladium in 15 ml of tetrahydrofuran and 5 ml of N,N-dimethylformamide, heated to 55° C. under an atmosphere of carbon monoxide is added a solution of 1.40 g of tri-n-butyltin hydride in 2.5 ml of toluene over 6 hours via a syringe pump. After the addition is complete the reaction is allowed to cool to room temperature, diluted with brine and extracted with chloroform. The combined organics are concentrated in vacuo and the resulting residue triturated with ether. The precipitate is collected by filtration and purified by flash chromatography on silica gel, eluting with 1:1 ethyl acetate-hexanes to give 0.35 g of the desired product, m.p. 242°-244° C. 
     EXAMPLE 20 
     2-Butyl-6-[(trimethylsilyl)ethylnyl]-4(1H)-quinazolinone 
     To a solution of 1.0 g of 2-butyl-1,4-dihydro-4-oxo-6-iodo-quinazolinone 0.043 g of bis(triphenylphosphine)palladium (II) chloride and 5.8 mg of copper (I) iodide in 5.0 ml of N,N-dimethylformamide and 5.0 ml of triethylamine is added 0.36 g of (trimethylsilyl)acetylene. The resulting reaction mixture is heated at 45° C. for 1 hour and them 65° C. for 5 hours. Upon cooling, the reation mixture is concentrated in vacuo and the residue is purified by flash chromatography on silica gel, eluting with 1:3 ethyl acetate-hexane to yield 0.75 g of the desired product as a white solid. CI MASS SPEC 299(M + ). 
     EXAMPLE 21 
     2-Butyl-6-[(trimethylsilyl)ethylnyl]-7-fluoro-4(1H)-quinazolinone 
     The compound is prepared using the experimental conditions of Example 20 starting from 7-fluoro-6-bromo-2-butyl-4(1H)-quinazolinone, m.p 192° C. 
     EXAMPLE 22 
     2-Butyl-6-ethylnyl-4(1H)-quinazolinone 
     To a solution of 0.70 g of 2-butyl-6-[(trimethylsilyl)ethynyl]-4(1H)-quinazolinone in 20 ml of methanol and 20 ml of tetrahydrofuran is added 10.0 ml of 1.0N sodium hydroxide solution. The reaction is stirred at room temperature for 2 hours and then diluted with 5% hydrochloric acid solution until the pH is 2. The resulting tan precipitate is collected by filtration and dried in vacuo to yield 0.50 g of the desired product. CI MASS SPEC 227(MH + ). 
     EXAMPLE 23 
     6-Acetyl-2-butyl-4(1H)-quinazolinone 
     To a solution of 1.20 g of 2-butyl-6-ethynyl-4(1H)-quinazolinone in 90 ml of acetic acid is added 0.45 g of mercuric sulfate, 0.9 ml of water and 0.3 ml of sulfuric acid. The reaction mixture is heated at reflux for 5 hours, cooled to room temperature and quenched with 150 ml of water. The resulting mixture is concentrated in vacuo, diluted with 150 ml of water and extracted with 6:1 chloroform-methanol. The combined organics are dried over magnesium sulfate, filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with 1:1 ethyl acetate-hexanes to give 0.67 g of the desired product as a white solid. CI MASS SPEC 245(MH + ). 
     EXAMPLE 24 
     2-Butyl-6-(1-hydroxy-1-methylethyl)-4(1H)-quinazolinone 
     To a solution of 4.00 g 6-acetyl-2-butyl-4(1H)-quinazolinone in 250 ml of dry tetrahydrofuran, cooled to 0° C., is added dropwise 16.4 ml of 3.0M methylmagnesium bromide in diethyl ether. The reaction is stirred at 0° C. for 0.5 hours and then allowed to warm to room temperature followed by quenching with 100 ml of saturated ammonium chloride solution. The mixture is diluted with 50 ml of water and extracted with ethyl acetate. The combined organic layers are washed with brine, dried over anhydrous magnesium sulfate, filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with 100:0.25 chloroform-methanol to give 2.75 g of the desired product as a white solid. CI MASS SPEC 261(MH + ). 
     EXAMPLE 25 
     2-Butyl-6-(1-hydroxyethyl)-4(1H)-quinazolinone 
     To a suspension of 0.102 g of 6-acetyl-2-butyl-4(1H)quinazolinone in 10.0 ml of ethanol is added 0.015 g of sodium borohydride. The reaction mixture is stirred for 1.5 hours at room temperature and then diluted with 50 ml of water. The aqueous layer is extracted with 5:1 chloroform-methanol and the combined organics dried over magnesium sulfate, filtered and concentrated in vacuo to yield 0.103 g of the desired product. CI MASS SPEC 247(MH + ). 
     EXAMPLE 26 
     2-Butyl-6-ethyl-4(1H)-quinazolinone 
     To a suspension of 0.278 g of 2-butyl-6-ethynyl-4(1H)quinazolinone in 8 ml of pyridine is added 0.080 g of 5% palladium-on-barium sulfate. The reaction is stirred under a hydrogen atmosphere for 48 hours, filtered, concentrated in vacuo and the residue purified by flash chromatography on silica gel eluting with ethyl acetate-hexanes to give 0.179 g of the desired product. CI MASS SPEC 231(MH + ). 
     EXAMPLE 27 
     Methyl 2-butyl-1,4-dihydro-4-oxo-6-quinazolinecarboxylate 
     To a solution of 1.00 g of 2-butyl-1,4-dihydro-4-oxo-6-iodoquinazoline and 6.0 ml of triethylamine in 25 ml of methanol and 5 ml of N,N-dimethylformamide is added 0.275 g of bis(triphenylphosphine)palladium (II) chloride. The reaction mixture is heated at reflux under an atmosphere of carbon monoxide for 16 hours, then allowed to cool and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with 1:1 ethyl acetate-hexanes to give 0.389 g of the desired product as a white solid. CI MASS SPEC 261(MH + ). 
     EXAMPLE 28 
     2-Butyl-6-(1-hydroxy-1-methylethyl)-4(1H)-quinazolinone 
     To a solution of 0.075 g of methyl 2-butyl-1,4-dihydro-4-oxo-6-quinazolinecarboxylate in 5 ml of dry tetrahydrofuran, cooled to 0° C., is added dropwise 0.51 ml of a solution of 3.0M methylmagnesium bromide in diethyl ether. The reaction is stirred at 0° C. for 0.5 hours and then at room temperature for 1 hour followed by quenching with 10 ml of saturated ammonium chloride solution. The resulting reaction mixture is diluted with 10 ml of water and extracted with ethyl acetate. The combined organics are dried over magnesium sulfate, filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with 100:0.25 chloroform-methanol to yield 0.055 g of the desired product as a white solid, m.p. 190°-192° C. 
     EXAMPLE 29 
     2-Butyl-6-(1-methylethenyl)-4(1H)-quinazolinone 
     To a suspension of 3.66 g of methyltriphenylphosphonium bromide in 30 ml of dry tetrahydrofuran, cooled to -78° C., is added dropwise 5.9 ml of a 1.73M solution of n-butyllithium in hexanes. Following complete addition, the reaction mixture is allowed to warm to room temperature and stirred for 15 minutes, until all the phosphonium bromide is dissolved. The reaction mixture is then recooled to -78° C. and a suspension of 6-acetyl-2-butyl-4(1H)-quinazolinone in 15 ml of dry tetrahydrofuran is added. The reaction is allowed to warm to room temperature and stirred for 24 hours followed by quenching with saturated ammonium chloride solution. After diluting with 10 ml of water, the aqueous layer is extracted with chloroform and the combined organics dried over magnesium sulfate, filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with 1:2 ethyl acetate-hexanes to give 0.23 g of the desired product as a white solid. CI MASS SPEC 243(MH +  ). 
     EXAMPLE 30 
     2-Butyl-6-(hydroxyphenylmethyl)-4(1H)-quinazolinone 
     To a stirred solution of 2.00 g of 2-butyl-1,4-dihydro-4-oxo-6-quinazoline-carboxaldehyde in 100 ml of tetrahydrofuran, cooled at 0° C., is added 13.0 ml of 2.0M phenyllithium and stirring continued for 1  hour. The cooling is removed and the reaction allowed to reach room temperature followed by an additional 30 minutes at room temperature. The reaction is diluted with saturated ammonium chloride solution and extracted with ethyl acetate. The organic layer is dried, evaporated to a residue, which is purified by chromatography on silica gel by elution with 0.25:100 methanol-chloroform to give 0.932 g of the desired product. CI MASS SPEC 309(MH + ). 
     EXAMPLE 31 
     2-Butyl-6-(1-hydroxyethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     A suspension of 2.50 g of 2-butyl-6-(1-hydroxyethyl)-4(1H)-quinazolinone, 6.79 g of 5-[4&#39;-(bromomethyl)[1,1&#39;-biphenyl]-2-yl]-1-(triphenylmethyl)-1H-tetrazole and 4.20 g of anhydrous potassium carbonate in 225 ml of dry acetone is heated at reflux for 16 hours. The reaction mixture is allowed to cool to room temperature, filtered and the filtrate evaporated in vacuo. The residue is purified by high pressure liquid chromatography on silica gel by eluting with 1:2 ethyl acetate-hexanes to afford 4.25 g of the desired product as a white solid, FAB M+H 723. 
     Examples 32-48 in Table II are prepared under substantially the same alkylation conditions as Example 31 from the appropriately substituted quinazolinone starting materials. 
     
                                           TABLE II__________________________________________________________________________ ##STR28##Ex.                       FAB Low ResolutionNo.   R.sup.5R.sup.6   R.sup.7            R.sup.8              X      Mass Spectrum__________________________________________________________________________32 H CH(OH)CH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                     723(M + H)33 H CH.sub.3  H H (CH.sub.2).sub.3 CH.sub.3                     693(M + H)34 H CH.sub.2 CH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                     707(M + H)35 H CH.sub.2 OH          H H (CH.sub.2).sub.3 CH.sub.3                     709(M + H)36 H H         H H (CH.sub.2).sub.3 CH.sub.3                     665(M + H)37 H CH(OH)CH.sub.2 CH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                     737(M + H)38 H (CH.sub.3).sub.2 OH          H H (CH.sub.2).sub.3 CH.sub.3                     737(M + H)39 H CH.sub.3  H H (CH.sub.2).sub.3 CH.sub.3                     693(M + H)40 H H         H H (CH.sub.2).sub.3 CH.sub.3                     665(M + H)41 H CO.sub.2 CH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                     737(M + H)42 H ##STR29##          H H (CH.sub.2).sub.3 CH.sub.3                     721(M + H)43 H CH(OCH.sub.3)CH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                     737(M + H)44 H CH(OCH.sub.3)C.sub.6 H.sub.5          H H (CH.sub.2).sub.3 CH.sub.3                     799(M + H)45 H CH(OH)C.sub.6 H.sub.5          H H (CH.sub.2).sub.3 CH.sub.3                     807(M + Na)46 H CH(OCH.sub.3)C.sub.2 H.sub.5          H H  (CH.sub.2).sub.3 CH.sub.3                     751(M + H)47 H C(CH.sub.3).sub.2 OCH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                     751(M + H)48 H Cl        H H (CH.sub.2).sub.3 CH.sub.3                     609(M + H)__________________________________________________________________________ 
    
     EXAMPLE 49 
     2-Butyl-6-(1-hydroxyethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)-[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     To a suspension of 2.00 g of 2-butyl-6-(1-hydroxyethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;biphenyl]-4-yl]methyl]-4(3H)-quinazolinone in 70 ml of 3:1 acetone-water is added one drop of 5% aqueous hydrochloric acid solution. The mixture is then heated at reflux for 16 hours. After cooling, the reaction mixture is concentrated in vacuo and the residue purified by flash chromatography on silica gel by elution with 9:1 chloroform-methanol to afford 0.915 g of the desired product as a white solid, m.p. 146°-147° C. 
     Examples 50-57 in Table III are prepared under substantially the same conditions as Example 49 from the appropriately substituted quinazolinone starting materials. 
     
                                           TABLE III__________________________________________________________________________ ##STR30##Ex.No.   R.sup.5R.sup.6   R.sup.7            R.sup.8              X      MP °C.                          FAB MS__________________________________________________________________________50 H H         H H (CH.sub.2).sub.3 CH.sub.3                     9251 H C(CH.sub.3).sub.2 OH          H H (CH.sub.2).sub.3 CH.sub.3                     156-15852 H CH(OH)CH.sub.2 CH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                     138-14053 H CH.sub.2 OH          H H (CH.sub.2).sub.3 CH.sub.3                     126-12854 H CH(OH)C.sub.6 H.sub.5          H H (CH.sub.2).sub. 3 CH.sub.3                          543(M + H)55 H CH(OCH.sub.3)C.sub.2 H.sub.5          H H (CH.sub.2).sub.3 CH.sub.3                          509(M + H)56 H C(C.sub.6 H.sub.5)OCH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                          557(M + H)57 H C(CH.sub.3).sub.2 OCH.sub.3          H H (CH.sub.2).sub.3 CH.sub.3                          509(M + H)__________________________________________________________________________ 
    
     EXAMPLE 58 
     6-[1-(Acetyloxy)ethyl]-2-butyl-3-[[2&#39;-(1H-tetrazol-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     To a solution of 1.00 g of 2-butyl-6-(1-hydroxyethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)-[1,1&#39;-biphenyl]4-yl]methyl-4(3H)-quinazolinone in 2.0 ml of acetic anhydride at room temperature is added 2 drops of dry pyridine. The reaction is stirred at room temperature for 16 hours then evaporated in vacuo. The residue is purified by flash chromatography on silica gel eluting with 95:5 chloroform-methanol to yield 0.059 g of the desired product as a white solid. FAB MASS SPEC 523(M+H). 
     EXAMPLE 59 
     2-Butyl-6-(1-methoxyethyl)-4-[[2&#39;-(1H-tetrazol)-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     A mixture of 0.300 g of 2-butyl-6-(1-methoxyethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl]-[1,1&#39;-biphenyl]-4-yl]methyl-4(3H)-quinazolinone in 20 ml of 3:1 acetone-water containing 1 drop of 5% hydrochloric acid is heated at reflux for 16 hours and evaporated to a residue. The residue is purified on silica gel eluting with 95.5 chloroform-methanol to provide 0.171 g of the product as a white solid, mp 154°-156° C. 
     EXAMPLE 60 
     2-Butyl-6-(1-methoxyethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol)-5-yl][1,1&#39;-biphenyl]-4-yl]methyl-4(3H)-quinazolinone 
     To a suspension of 0.044 g of a 60% dispersion of sodium hydride in mineral oil and 0.345 ml of methyl iodide in 5.0 ml of dry tetrahydrofuran at room temperature is added 0.400 g of 2-butyl-6-(1-hydroxyethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl]-[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone in one portion. The reaction is stirred at room temperature for 18 hours and then poured onto a pad of silica gel. Elution with 1:3 ethyl acetate-hexanes and fractions containing the desired product evaporated to provide 0.356 g of a white solid. FAB MASS SPEC 737(M+H). 
     EXAMPLE 61 
     2-Butyl-6-(1-hydroxy-1-methylethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone monosodium salt 
     A mixture of 0.400 g of 2-Butyl-6-(1-hydroxy-1-methylethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone monosodium salt, 10 ml of methanol and 0.810 ml of 1N sodium hydroxide is stirred at room temperature for 1 hour and evaporated in vacuo to a residue which is triturated with ether, filtered and the cake air dried to give the desired product as a solid. FAB MASS SPEC 517(M+H). 
     EXAMPLE 62 
     2-Butyl-6-(hydroxymethyl)-3-[[2&#39;-(1H-tetrazol-5-yl) [1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone monosodium salt 
     Following the procedure of Example 182 and using 2-Butyl-6-(1-hydroxy-1-methylethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone affords the product of the Example. 
     EXAMPLE 63 
     2-Butyl-6-(1-methoxy-1-methylethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl-4(3H)-quinazolinone 
     To a suspension of 0.049 g of a 60% oil dispersion of sodium hydride in 4.5 ml of THF is added 0.76 ml of methyl iodide followed by 0.45 g of 2-butyl-6-(1-hydroxy-1-methylethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)-[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone. The reaction mixture is stirred at room temperature for 24  hours and then an additional 0.05 g of 60% sodium hydride and 0.80 ml of methyl iodide are added. The reaction is stirred at room temperature for another 24 hours and then quenched with saturated ammonium chloride solution and extracted with ether. The organics are dried over MgSO 4 , filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with 1:5 ethyl acetate/hexanes to provide 0.397 g of the desired product as a white solid. FAB MASS SPEC 751(M+H). 
     EXAMPLE 64 
     2-Butyl-6-(1-methoxy-1-methylethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     The product of the Example is prepared using the conditions of Example 111 and 0.397 g of 2-Butyl-6-(1-methoxy-1-methylethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl-4(3H)-quinazolinone to give 0.188 g of the desired product as a white solid following chromatography on silica gel by elution with 50:50:5:0.1 ethyl acetate/hexanes/methyl alcohol/acetic acid. FAB MASS SPEC 509(M+H). 
     EXAMPLE 65 
     2-Butyl-6-(methoxyphenylmethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-(3H)-quinazolinone 
     To a solution of 0.398 ml of methyl iodide in 5.0 ml of THF is added 0.851 g of 60% sodium hydride followed by 0.500 g of 2-butyl-6-(hydroxyphenylmethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone. The reaction mixture is stirred for 16 hours at room temperature, then quenched with saturated NH 4  Cl solution and extracted with ether. The organics are dried over MgSO 4 , filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with ethyl acetate/hexanes (1:5) to provide 0.434 g of FAB MASS SPEC 799(M+H). 
     EXAMPLE 66 
     2-Butyl-6-(methoxyphenylmethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     The product of the Example is prepared using the conditions of Example 49 and 0.413 g of 2-Butyl-6-(methoxyphenylmethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-(3H)-quinazolinone to give 0.192 g of the desired product as a white solid following chromatography on silica gel by elution with 50:50:5:0.1 ethyl acetate/hexanes/methyl alcohol/acetic acid. FAB MASS SPEC 557(M+H). 
     EXAMPLE 67 
     2-Butyl-6-(1-methoxypropyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     To a solution of 0.556 ml of methyl iodide in 5.0 ml of THF is added 0.071 g of 60% sodium hydride followed by 0.657 g of 2-butyl-6-(1-hydroxypropyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]4(3H)-quinazolinone. The reaction is stirred for 16 hours at room temperature, then quenched with saturated NH 4  Cl solution and extracted with ether. The organics are dried over MgSO 4 , filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel, eluting with 1:5 ethyl acetate/hexanes to provide 0.59 g of the desired product as a white solid. FAB MASS SPEC 751(M+H). 
     EXAMPLE 68 
     2-Butyl-6-(1-methoxypropyl)-3-[[2&#39;-(1H-tetrazol-5-yl) [1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     The product of the Example is prepared using the conditions of Example 111 and 0.58 g of 2-Butyl-6-(1-methoxypropyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone to give 0.326 g of the desired product as a white solid following chromatography on silica gel by elution with 50:50:5:0.1 ethyl acetate/hexanes/methyl alcohol/acetic acid. FAB MASS SPEC 509(M+H). 
     EXAMPLE 69 
     1-Amino-3-fluoro-4-bromo-benzoic acid 
     To a solution of 3.0 g of 4-fluoroanthranilic acid in 250 ml of glacial acetic acid is slowly added a solution of 3.2 g of bromine in 10 ml of acetic acid. The reaction mixture is stirred at room temperature for 8 hours and poured into 500 ml of water. The resulting precipitate is filtered off and the cake crystallized from methyl alcohol to give 3.4 g of the desired product, m.p. 180°. 
     EXAMPLE 70 
     6-Bromo-2-butyl-7-fluoro-4(1H)-quinazolinone 
     Following the procedure of Example 1 and using 3.0 g of 1-amino-3-fluoro-4-bromo-benzoic acid, 100 ml of valeric anhydride and 200 ml of ammonium hydroxide affords the product of the Example, m.p. 225° C. 
     EXAMPLE 71 
     2-Butyl-7-fluoro-6-[(trimethylsilyl)ethynyl]-4(1H)-quinazolinone 
     Using the procedure of Example 20 and 6-bromo-2-butyl-7-fluoro-4(1H)-quinazolinone, the product of the Example is obtained, m.p. 192° C. 
     EXAMPLE 72 
     2-Butyl-6-ethynyl-7-fluoro-4(1H)-quinazolinone 
     A mixture of 1.0 g of 2-butyl-7-fluoro-6-[(trimethylsilyl)ethynyl]-4(1H)-quinazolinone, 20 ml of 1N sodium hydroxide and 25 ml of methyl alcohol is heated at 60° C. for 5 hours then evaporated in vacuo. The residue is dissolved in 100 ml of water and acidified. The resulting solid is collected and dried to afford 700 mg of the desired product as a yellow solid, m.p. 218° C. 
     EXAMPLE 73 
     3-[(4-Bromophenyl)methyl]-2-butyl-6-(1-hydroxy-1-methylethyl)-4(3H)-quinazolinone 
     To a solution of 1.37 g of 2-butyl-6-(1-hydroxy-1-methylethyl)-4(1H)-quinazolinone in 115 ml of acetone is added 1.58 g of 4-bromobenzyl bromide and 2.18 g of anhydrous potassium carbonate. The resulting suspension is heated to reflux for 16 hours. The reaction mixture is then allowed to cool to room temperature, filtered and the filtrate is concentrated vacuo. The residue is purified by HPLC eluting with ethyl acetate/hexanes (1:3) to provide the desired product. 
     Examples 74-84 in Table VI are prepared under substantially the same alkylation conditions as Example 73 from the appropriately substituted quinazolinone starting materials. 
     
                       TABLE VI______________________________________ ##STR31##Ex.No.  R.sup.5 R.sup.6       R.sup.7                            R.sup.8                                X______________________________________74   H       I             H     H   (CH.sub.2).sub.3 CH.sub.375   H       CH(OH)CH.sub.3                      H     H   (CH.sub.2).sub.3 CH.sub.376   H       CH.sub.3      H     H   (CH.sub.2).sub.3 CH.sub.377   H       CH.sub.2 OH   H     H   (CH.sub.2).sub.3 CH.sub.378   H       H             H     H   (CH.sub.2).sub.3 CH.sub.379   H       CH(OH)CH.sub.2 CH.sub.3                      H     H   (CH.sub.2).sub.3 CH.sub.380   H       C(CH.sub.3).sub.2 OH                      H     H   (CH.sub.2).sub.3 CH.sub.381   H       CH.sub.3      H     H   (CH.sub.2).sub.3 CH.sub. 382   H       H             H     H   (CH.sub.2).sub.3 CH.sub.383   H       CH(OH)C.sub.6 H.sub.5                      H     H   (CH.sub.2).sub.3 CH.sub.384   H       Cl            H     H   (CH.sub.2).sub.3 CH.sub.3______________________________________ 
    
     EXAMPLE 85 
     3-[(4-Bromophenyl)methyl]-2-butyl-6-(1-methoxy-1-methylethyl)-4(3H)-quinazolinone 
     To a solution of 0.186 g of 60% sodium hydride and 2.90 ml of iodomethane in THF at room temperature is added 1.00 g of 3-[(4-bromophenyl)methyl]-2-butyl-6-(1-hydroxy-1-methylethyl)-4(3H)-quinazolinone. The reaction mixture is stirred overnight at room temperature and then quenched with ammonium chloride solution and diluted with water. The aqueous layer is extracted with ether and the combined organics are dried over MgSO 4 , filtered and concentrated in vacuo. The residue is purified by flash chrmoatography eluting with ethyl acetate/hexanes (1:3) to provide the desired product. 
     Examples 86-89 in Table VII are prepared under substantially the same alkylation conditions as Example 85 from the appropriately substituted quinazolinone starting materials. 
     
                       TABLE VII______________________________________ ##STR32##Ex.No.   R.sup.5       R.sup.6         R.sup.7                           R.sup.8                                 X______________________________________86    H     CH(OCH.sub.3)CH.sub.3                       H   H     (CH.sub.2).sub.3 CH.sub.387    H     CH(OCH.sub.3)C.sub.6 H.sub.5                       H   H     (CH.sub.2).sub.3 CH.sub.388    H     CH(OCH.sub.3)C.sub.2 H.sub.5                       H   H     (CH.sub.2).sub.3 CH.sub.389    H     C(CH.sub.3).sub.2 OCH.sub.3                       H   H     (CH.sub.2).sub.3 CH.sub.3______________________________________ 
    
     EXAMPLE 90 
     2-Butyl-6-(1-methoxy-1-methylethyl)-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     To a suspension of 0.41 g of magnesium turnings in 50 ml of THF is added a catalytic amount of iodine followed by 1.00 g of the 5-(2-bromophenyl)-1-(triphenylmethyl)-1H-tetrazole in 10 ml of THF. The reaction mixture is heated to reflux until the Grignard formation is initiated as indicated by the disappearance of the iodine color. The remaining 6.94 g of the bromide in 70 ml of THF is then added to the reaction at a rate sufficient to maintain a gentle reflux. The reaction is then stirred at room temperature for 4 hours following the completion of the bromide addition. 
     To a solution of 4.08 g of the 3-[(4-bromophenyl)methyl]-2-butyl-6-(1-methoxy-1-methylethyl)-4(3H)-quinazolinone and 71 mg of 1,4-bis(diphenylphosphino)butane palladium (II) chloride in 100 ml of THF is added the Grignard solution described above and the resulting solution is heated to reflux for 2 hours, following the method of Kumada (Tet. Letters, 52, 5319 (1981) . After cooling to room temperature, the reaction is quenched with water and dilute sodium hydroxide solution and then extracted with ether. The combined organics are dried over MgSO 4 , filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel eluting with ethyl acetate/hexanes (1:3) to provide the desired product. 
     EXAMPLES 91-94 in Table VIII are prepared under substantially the same coupling conditions as Example 90 from the appropriately substituted quinazolinone starting materials. 
     
                       TABLE VIII______________________________________ ##STR33##Ex.No.  R.sup.5 R.sup.6       R.sup.7                            R.sup.8                                X______________________________________91   H       CH(OCH.sub.3)CH.sub.3                      H     H   (CH.sub.2).sub.3 CH.sub.392   H       CH(OCH.sub.3)C.sub.6 H.sub.5                      H     H   (CH.sub.2).sub.3 CH.sub.393   H       CH(OCH.sub.3)C.sub.2 H.sub.5                      H     H   (CH.sub.2).sub.3 CH.sub.394   H       C(CH.sub.3).sub.2 OCH.sub.3                      H     H   (CH.sub.2).sub.3 CH.sub.3______________________________________ 
    
     EXAMPLE 95 
     3-[(4-Bromophenyl)methyl]-2-butyl-6-[1-[[(1,1-dimethylethyl)dimethylsilyl]oxy]-1-methylethyl]-4(3H)-quinazolinone 
     To a solution of 1.00 g of 3-[(4-bromophenyl) methyl]-2-butyl-6-(1-hydroxy-1-methylethyl)-4(3H)-quinazolinone in 2.0 ml of DMF is added 0.438 g of t-butyldimethylsilyl chloride followed by 0.4 g of imidazole. The reaction mixture is stirred at room temperature for 18 hours and then diluted with water and extracted with ether. The combined ether extracts are dried over MgSO 4 , filtered and concentrated in vacuo. The residue was purified by flash chromatography eluting with ethyl acetate/hexanes (1:5) to provide the desired product as a white solid. 
     Examples 96-100 in Table IX are prepared under substantially the same conditions as Example 95 from the appropriately substituted quinazolinone starting materials. 
     
                       TABLE IX______________________________________ ##STR34##Ex.No.  R.sup.5       R.sup.6          R.sup.7                            R.sup.8                                 X______________________________________96   H      CH(OTBDMS)CH.sub.3                        H   H    (CH.sub.2).sub.3 CH.sub.397   H      CH.sub.2 OTBDMS  H   H    (CH.sub.2).sub.3 CH.sub.398   H      CH(OTBDMS)CH.sub.2 CH.sub.3                        H   H    (CH.sub.2).sub.3 CH.sub.399   H      C(CH.sub.3).sub.2 OTBDMS                        H   H    (CH.sub.2).sub.3 CH.sub.3100  H      CH(OTBDMS)C.sub.6 H.sub.5                        H   H    (CH.sub.2).sub.3 CH.sub.3______________________________________ *TBDMS = tbutyldimethylsilyl 
    
     EXAMPLE 101 
     2-Butyl-6-[1-[[(1,1-dimethylethyl]dimethylsilyl]oxy]-1-methylethyl]-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     To a suspension of 0.41 g of magnesium turnings in 50 ml of THF is added a catalytic amount of iodine followed by 1.00 g of the 5-(2-bromophenyl)-1-(triphenylmethyl)-1H-tetrazole in 10 ml of THF. The reaction mixture is heated to reflux until the Grignard formation is initiated as indicated by the disappearance of the iodine color. The remaining 6.94 g of the bromide in 70 ml of THF is then added to the reaction at a rate sufficient to maintain a gentle reflux. The reaction is then stirred at room temperature for 4 hours following the completion of the bromide addition. 
     To a solution of 5.00 g of 3-[(4-bromophenyl) methyl]-2-butyl-6-[1-[[(1,1-di-methylethyl)dimethylsilyl]oxy]-1-methylethyl]-4(3H)-quinazolinone and 71 mg of 1,4-bis(diphenylphosphino)butane palladium (II) chloride in 100 ml of THF is added the Grignard solution described above and the resulting solution is heated to reflux for 2 hours, fillowing the method of Kumada (Tet. Letters, 52, 5319 (1981)). After cooling to room temperature, the reaction is quenched with water and dilute sodium hydroxide solution and then extracted with ether. The combined organics are dried over MgSO 4 , filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel eluting with ethyl acetate/hexanes (1:3) to provide the desired product. 
     Examples 102-106 in Table X are prepared under substantially the same conditions as Example 101 from the appropriately substituted quinazolinone starting materials. 
     
                       TABLE X______________________________________ ##STR35##Ex.No.  R.sup.5       R.sup.6          R.sup.7                            R.sup.8                                 X______________________________________102  H      CH(OTBDMS)CH.sub.3                        H   H    (CH.sub.2).sub.3 CH.sub.3103  H      CH.sub.2 OTBDMS  H   H    (CH.sub.2).sub.3 CH.sub.3104  H      CH(OTBDMS)CH.sub.2 CH.sub.3                        H   H    (CH.sub.2).sub.3 CH.sub.3105  H      C(CH.sub.3).sub.2 OTBDMS                        H   H    (CH.sub.2).sub.3 CH.sub.3106  H      CH(OTBDMS)C.sub.6 H.sub.5                        H   H    (CH.sub.2).sub.3 CH.sub.3______________________________________ *TBDMS = tbutyldimethylsilyl 
    
     EXAMPLE 107 
     2-Butyl-6-(1-hydroxy-1-methylethyl)-3-[[2&#39;-[1-triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone 
     To a solution of 0.50 g of 2-butyl-6-[1-[[(1,1-dimethylethyl)dimethylsilyl]oxy]-1-methylethyl]-3-[[2&#39;-[1-(triphenylmethyl)-1H-tetrazol-5-yl][1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone in 15.0 ml of THF is added 0.88 ml of a 1.0M solution of tetrabutylammonium fluoride in THF. The reaction is stirred at room temperature for 1 hour and then poured into 100 ml of water. The aqueous layer is extracted with ether and the combined organics are washed with brine, dried over MgSO 4 , filtered and concentrated in vacuo. The residue is purified by flash chromatography on silica gel eluting with ethyl acetate/hexanes (1:3) to provide the desired product. 
     EXAMPLES 108-112 in Table XI are prepared under substantially the same conditions as Example 107 from the appropriately substituted quinazolinone starting materials. 
     
                       TABLE XI______________________________________ ##STR36##Ex.No.  R.sup.5 R.sup.6        R.sup.7                           R.sup.8                                 X______________________________________108  H       CH(OH)CH.sub.3 H   H     (CH.sub.2).sub.3 CH.sub.3109  H       CH.sub.2 OH    H   H     (CH.sub.2).sub.3 CH.sub.3110  H       CH(OH)CH.sub.2 CH.sub.3                       H   H     (CH.sub.2).sub.3 CH.sub.3111  H       C(CH.sub.3).sub.2 OH                       H   H     (CH.sub.2).sub.3 CH.sub.3112  H       CH(OH)C.sub.6 H.sub.5                       H   H     (CH.sub.2).sub.3 CH.sub.3______________________________________ 
    
     EXAMPLE 113 
     5-(2-Bromophenyl)-1-(trimethylstannyl)-1H-tetrazole 
     To a solution of 1.50 g of o-bromobenzonitrile in 8.0 ml of toluene is added 1.70 g of trimethyltin azide. The reaction mixture is heated to reflux for 18 hours and then allowed to cool to room temperature. The resulting white precipitate is isolated by filtration and used without purification in the next step. 
     EXAMPLE 114 
     5-(2-Bromophenyl)-1H-tetrazole 
     To a solution of 1.0 g of 5-(2-bromophenyl)-1-(trimethylstannyl)-1H-tetrazole in toluene/THF (10:1) at room temperature is added HCl gas, via a bubbler. Gas addition is continued for 5 minutes after the appearance of a precipitate and the solid is then isolated by filtration and washed with hexanes. 
     EXAMPLE 115 
     5-(2-Bromophenyl)-1-(triphenylmethyl)-1H-tetrazole 
     To a solution of 0.50 g of 5-(2-bromophenyl)-1H-tetrazole in 20 ml of CH 2  Cl 2  is added 0.65 g of triphenylmethyl chloride followed by 0.37 ml of triethylamine. The solution is refluxed for 2.5 hours, cooled to room temperature and then washed with water, dried over MgSO 4  and concentrated in vacuo. The residue is purified by flash chromatography eluting with ethyl acetate/hexanes (1:5) to provide the desired product. 
     EXAMPLE 116 
     2-butyl-6-(1-methoxy-1-methylethyl)-3-[[2&#39;-(1H-tetrazol-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl]-4(3H)-quinazolinone, sodium salt 
     To a solution of 2.488 g of the free tetrazole from Example 57 in 60 ml of methanol is added 5.036 ml of 1.0N sodium hydroxide solution. The mixture is stirred at room temperature for 1 hour and then concentrated in vacuo. The residue is titrated with hexanes, filtered and dried in vacuo to provide 2.29 g of the product as a white solid. 
     EXAMPLE 117 
     2-Butyl-6-(1-methoxyethyl)-4-[[2&#39;-(1H-tetrazol)-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl-4(3H)-quinazolinone, sodium salt 
     To a solution of 2.156 g of the free tetrazole from Example 59 in 60 ml of methanol is added 4.359 ml of 1.0N Sodium Hydroxide solution. The mixture is stirred at room temperature for 1 hour and then concentrated in vacuo. The residue is titrated with hexanes, filtered and dried in vacuo to provide 2.09 g of the product as a white solid. 
     EXAMPLE 118 
     2-Butyl-6-(methoxymethyl)-4-[[2&#39;-(1H-tetrazol)-5-yl)[1,1&#39;-biphenyl]-4-yl]methyl-4(3H)-quinazolinone 
     To a stirred solution of NaH (500 mg) and primary alcohol from Example 53 in dry THF (35 ml) at 0° C., is added CH 3  I (1 ml). The reaction mixture is stirred at room temperature for 4 hours. The reaction mixture is then carefully poured over crushed ice and extracted with chloroform. The organic layer is washed well with water; dried and concentrated. The spongy solid obtained is dissolved in acetone (25 ml) and 5N HCL (3 ml) is added. The reaction mixture is stirred at room temperature for 3 hours. The reaction mixture is then concentrated and the product is purified by column chromatography. Yield: 8.5 mg; m.p: 85° C. 
     Utility 
     The performance of the novel compounds of the present invention are shown in the following In Vitro test. The results of this test for representative compounds of the present invention are shown in Table IV. 
     Angiotensin II Antagonists In Vitro Tests 
     The source of the angiotensin II receptors utilized in the screen is from rat adrenocortical microsomes. The cortices are placed in ice cold sucrose buffer (0.2 m sucrose, 1 mm EDTA, 10 mm Trizma base, pH 7.4) and homogenized in a chilled ground glass tissue grinder. The homogenate is centrifuged at 3000×g for 10 min. and the resultant supernatant is decanted through cheesecloth and centrifuged at 12,000×g for 3 min. The resulting supernatant is then centrifuged at 1000,000×g for 60 min. and the pellet resuspended in assay buffer (0.25% bovine serum albumin, 5 mm MgCl 2 , 50 mm Trizma base, pH 7.2). Binding assays are performed by incubating aliquots of freshly prepared microsomes in the absence or presence of compound (40 uM final concentration). Ten minutes later,  3  H-angiotensin II is added to each tube (2 nM final concentration) and incubated for 60 minutes at 27° C. The reaction is terminated by the addition of 3  ml of cold assay buffer without albumin and the bound and free radioactivity is separated rapidly through glass-fiber filters prewetted with assay buffer. After two additional 3 ml rinses, the filters are placed in scintillation fluid and counted in a scintillation counter to determine trapped radioactivity. Compounds that displace 50% of the labelled angiotensin are considered active compounds and are then evaluated in concentration-response experiments to determine IC 50  values. The results ar shown in Table IV. 
     
                                           TABLE IV__________________________________________________________________________ ##STR37##                       Angiotensin II                       ReceptorEx. No.R.sup.5  R.sup.6   R.sup.7              R.sup.8                X      Binding IC.sub.50 (M)__________________________________________________________________________49   H CH(OH)CH.sub.3            H H (CH.sub.2).sub.3 CH.sub.3                       8.8 × 10.sup.-951   H C(CH.sub.3).sub.2 OH            H H (CH.sub.2).sub.3 CH.sub.3                       9.4 × 10.sup.-952   H CH(OH)CH.sub.2 CH.sub.3            H H (CH.sub.2).sub.3 CH.sub.3                       1.2 × 10.sup.-854   H (C.sub.6 H.sub.5)CHOH            H H (CH.sub. 2).sub.3 CH.sub.3                       &gt;1.0 × 10.sup.-555   H CH.sub.3 CH.sub.2 CHOCH.sub.3            H H (CH.sub.2).sub.3 CH.sub.3                       5.0 × 10.sup.-956   H (C.sub.6 H.sub.5)CHOCH.sub.3            H H (CH.sub.2).sub.3 CH.sub.3                       8.6 × 10.sup.-957   H (CH.sub.3).sub.2 COCH.sub.3            H H (CH.sub.2).sub.3 CH.sub.3                       6.4 × 10.sup.-958   H CH.sub.3 CHOOCCH.sub.3            H H (CH.sub.2).sub.3 CH.sub.3                       1.1 × 10.sup.-860   H CH.sub.3 (CH.sub.3 O)CH            H H (CH.sub.2).sub.3 CH.sub.3                       8.4 × 10.sup.-864   H (CH.sub.3).sub.2 COCH.sub.3            H H (CH.sub.2).sub.3 CH.sub.3                       6.4 × 10.sup.-9  H66   H (C.sub.6 H.sub.5)C(OCH.sub.3)            H H (CH.sub.2).sub.3 CH.sub.3                       8.6 × 10.sup.-9  H68   H (C.sub.2 H.sub.5)C(OCH.sub.3)            H H (CH.sub.2).sub.3 CH.sub.3                       5.0 × 10.sup.-9__________________________________________________________________________ 
    
     The compounds of this invention inhibit the action of AII. By administering a compound of this invention to a rat, and then challenging with angiotensin II, a blackage of the vasopressor response is realized. The results of this test on representative compounds of this invention are shown in Table V. 
     AII Challenge 
     Conscious Male Okamoto-Aoki SHR, 16-20 weeks old, weighing approximately 330 g are purchased from Charles River Labs (Wilmington, Mass.). Conscious rats are restrained in a supine position with elastic tape. The area at the base of the tail is locally anesthetized by subcutaneous infiltration with 2% procaine. The ventral caudal artery and vein are isolated, and a cannula made of polyethylene (PE) 10-20 tubing (fused together by heat) is passed into the lower abdominal aorta and vena cava, respectively. The cannula is secured, heparinized (1,000 I.U./ml), sealed and the wound is closed. The animals are placed in plastic restraining cages in an upright position. The cannula is attached to a Statham P23Db pressure transducer, and pulsatile blood pressure is recorded to 10-15 minutes with a Gould Brush recorder. (Chan et al., (Drug Development Res., 18:75-94, 1989). 
     Angiotensin II (human sequence, Sigma Chem. Co., St. Louis, Mo.) of 0.05 and 0.1 ug/kg i.v. is injected into all rats (predosing response). Then a test compound, vehicle or a known angiotensin II antagonist is administered i.v., i.p. or orally to each set of rats. The two doses of angiotensin II are given to each rat again at 30, 90 and 150 minutes post dosing the compound or vehicle. The vasopressor response of angiotensin II is measured for the increase in systolic blood pressure in mmHg. The percentage of antagonism or blockade of the vasopressor response of angiotensin II by a compound is calculated using the vasopressor response (increase in systolic blood pressure) of angiotensin II of each rat predosing the compound as 100%. A compound is considered active if at 30 mg/kg i.v. it antagonized at least 50% of the response. 
     The results are shown in Table V and FIG. 1. 
     
                                           TABLE V__________________________________________________________________________% INHIBITION (ANGIOTENSIN BLOCKAGE) OF ANGIOTENSIN II (AII)VASOPRESSOR RESPONSE     AII Control              Response                   Min. After PercentDose Dose         Before              After                   Dosing     InhibitionEx. No.mg/kg iv     g/kg iv         AII  AII  Compound                         Change                              (Average)__________________________________________________________________________     .05 195  240  0     45     .1  185  240        5549   1    .05 190  217  30    27   40     .1  185  225        40   2749   1    .05 170  195  45    25   45     .1  185  210        25   55     .05 170  210  90    40   11     .1  185  225        40   2749   2    .05 190  200  120   10   78     .1  185  220        35   36     .05 190  225  150   35   23     .1  190  230        40   27     .05 175  225  180   50   11     .1  175  230        55   0     .05 220  254  0     26     .05 205  235  0     30     0.1 223  272  0     44     0.1 205  250  0     4549        .05 253  253  30    010   .05 190  190  30    0    100     0.1 243  243  30    0     0.1 190  190  30    0    100     .05 228  228  90    0     .05 185  185  90    0    100     .05 185  230  0     45         185  225        40     1.  180  235        55         185  235        5050   30   .05 175  190  30    15   82         190  190        0     1.  170  195        25   76         190  190        0     .05 170  200  90    30   29         180  215        30     1.  180  210        30   52         185  205        20     .05 170  200  150   30   41         170  190        20     1.  185  220        35   48         170  190        20     .05 220  260  0     40     .05 200  232  0     32     0.1 215  265  0     50     0.1 200  240  0     4051   5*   .05 205  235  30    30     .05 185  210  30    25   30     0.1 200  240  30    40     0.1 190  215  30    25   26     .05 205  210  90    5     .05 180  193  90    13   75     0.1 200  225  90    25     0.1 180  195  90    15   55     .05 190  205  150   15     .05 180  190  150   10   63     0.1 190  217  150   27     0.1 180  200  150   20   46     .05 235  285  0     50     .1  225  285        60*51  1    .05 220  260  30    40   20     .1  220  275        55   8*51  1    .05 220  245  45    25   50     .1  220  260        40   33     .05 220  265  90    45   10     .1  210  275        65   8*51  2    .05 225  250  120   25   50 *sodium salt 
    
     
                   .1  215  265        50   17     .05 225  260  150   35   30     .1  225  270        45   25     .05 225  265  180   40   20     .1  235  280        45   25     .05 210  265  0     55     .05 205  255  0     50     0.1 215  275  0     60     0.1 210  265  0     5552   5 oral     .05 205  230  30    25     .05 190  215  30    25   52     0.1 205  240  30    35     0.1 190  220  30    30   43     .05 185  205  90    20     .05 185  200  90    15   66     0.1 185  205  90    20     0.1 185  206  90    21   63     .05 180  195  150   15     .05 175  180  150   15   71     0.1 175  201  150   26     0.1 180  200  150   20   60     .05 165  220  0     55         185  230        45     .1  175  220        45         190  240        5053   10   .05 155  157  10    2    83         140  155        15     .1  155  165        10   90         130  130        0     .05 155  160  30    5    85         135  145        10     .1  160  165        5    84         155  165        10     .05 160  175  60    15   65         140  160        20     .1  165  180        15   68         150  165        15     .05 200  245  0     45         175  222        47         185  230        45     .1  200  252        47         170  217        55         180  235        5553   10                 30     .05 210  245        35   17         175  215        40         185  225        40     .1  205  250        45   19         175  220        45         195  232        37                   6053        .05 192  237        45   27         170  190        20         175  210        35     .1  195  245        50   4         170  220        50         175  225        50                   90     .05 205  245        40   41         175  195        20         185  207        22     .1  200  250        50   56         175  210        35         177  225        48                   30     .05 207  225        18   76         167  190        23         180  210        30     .1  200  255        55   60         175  197        22         180  223        43                   60     .05 200  240        40   46         170  180        10         200  225        2553        .1  207  250        43   44         170  190        20         190  230        40                   90     .05 195  235        40   35         165  180        15         180  215        35     .1  200  250        50   26         175  190        15         180  230        50     .05 210  275  0     65         230  270        40     .1  210  280        70         235  280        4553   6    .05 200  220  30    20   67         210  225        15     .1  205  220        15   70         210  230        20     .05 205  225  90    20   48         200  235        35     .1  205  240        35   30         205  250        4553   6    .025         235  265  0     30     .05 235  265        30     .1  235  265        3053   6    .025         210  225  30    15   50     .05 210  235        25   17     .1  215  250        35   17     .025         200  210  90    10     .05 190  225        35   17     .1  200  235        35   17     .05 187  240  0     53         213  260        47     .1  187  240        53         215  265        5053   3    .05 175  205  10    30   62         207  215        8     .1  175  215        40   42         205  225        20     .05 175  210  30    35   45         210  2340       20     .1  190  220        30   47         215  235        20     .05 175  215  60    40   30         210  240        30     .1  180  235        55   13         220  255        35     .05 210  270  0     60         225  265        40     .1  205  275        70         235  290        5553   3    .05 200  215  30    15   55         230  260        30     .1  195  215        20   44         220  270        50     .05 200  225  90    25   25         225  275        50     .1  200  235        35   13         225  277        5253   3    .05 175  190  30    15   60         225  250        25     .1  180  210        30   36         225  250        50     .05 200  250  0     50         210  265        55     .1  200  255        55         200  265        6553   3    .05 185  210  30    25   67         190  200        10     .1  185  205        20   67         190  210        20     .05 185  235  90    50   10         195  260        65     .1  190  235        45   17         200  255        55     .025         220  260        40         210  255        45     .05 220  265        45         210  260        50     .1  220  275        55         215  265        5053   3    .025         210  225  30    15   53         195  220        2553        .05 207  220        13   65         190  210        20     .1  210  235        25   52         190  215        25     .025         210  230  90    20   47         185  210        25     .05 210  240        30   35         190  222        32     .1  210  250        40         195  235        40   24     .05 190  235  0     45         210  255        65     .1  190  245        55         210  255        4554   1    .05 190  215  30    25   47         205  227        32     .1  195  230        35   30         210  245        3554   1    .05 190  207  45    17   75         205  215        10     .1  185  230        45   28         200  227        27     .05 190  217  90    27   49         210  240        30     .1  200  240        40   34         210  235        2554   2    .05 220  220  120   0    85         200  215        15     .1  202  222        20   64         200  215        15     .05 185  205  150   20   67         195  210        15     .1  190  215        25   54         195  215        20     .05 190  207  180   17   69         190  207        17     .1  190  220        30   50         190  210        2054   5 P.O.*     0.05         210  235  180   25   55     0.1 197  245        48   26 *oral dosage 
    
     
                   .05 200  255  0     55     .1  200  255        5555   30   .05 180  190  30    10   82     .1  180  195        15   73     .05 170  185  90    15   73     .1  170  175        5    91     .05 185  205  150   20   64     .1  190  195        5    91     .025         230  260  0     30     .05 225  270        45     .1  235  283        4857   1    .025         205  220  15    15   50     .05 220  225        5    89     .1  217  242        25   48     .025         215  230  30    15   50     .05 225  235        10   78     .1  225  260        35   27     .025         240  245  60    5    83     .05 240  255        15   67     .1  240  275        35   2757   2    .025         230  255  90    25   17     .05 225  235        10   78     .1  225  255        30   37     .025         205  265  120   60   100     .05 225  255        30   33     .1  235  255        20   5857   3    .025         210  225  150   15   50     .05 215  230        15   67     .1  215  240        25   48     .025         207  250  0     43         210  260        50     .05 215  253        38         210  265        55     .1  220  265        45         220  265        4557   *5   .025         205  210  30    5    94         205  205        0     .05 200  207        7    77         200  215        15     .1  198  215        17   64         200  215        15     .025         205  215  60    10   89         195  195        0     .05 215  215        0    87         193  205        12     .1  200  217        17   58         190  210        20     .025         195  200  120   5    94         200  200        0     .05 195  210        15   72         200  210        10     .1  192  220        28   40         200  225        25     .025         195  210  180   15   72         200  210        10     .05 200  215        15   68         200  215        15 *oral dosage 
    
     
                   .1  200  215        15   56         200  225        25     .025         220  220  240   0    100         190  190        0     .05 195  210        15   62         180  200        20     .1  220  235        15   49         185  215        30     .05 200  260  0     60         210  257        47     .1  205  260        55         200  260        6058   30   .05 195  205  30    10   87         222  225        3     .1  205  205        0    98         205  206        2     .05 190  190  90    0    85         210  225        15     .1  195  195        0    95         210  215        5     .05 180  190  150   10   85         200  205        5     .1  190  207        17   81         205  210        5     .05 235  285  0     50         230  290        60     .1  225  285        60         230  285        5560   1    .05 207  232  30    25   55         215  240        25     .1  215  260        45   17         210  260        5060   1    .05 195  205  45    10   82         215  225        10     .1  190  215        25   43         210  250        40     .05 210  230  90    20   58         210  235        25     .1  215  245        30   43         210  245        3560   2    .05 215  215  120   0    100         220  220        0     .1  210  220        10   86         220  225        5     .05 220  225  150   5    82         205  220        15     .1  215  225        10   52         205  250        45     .05 215  240  180   25   45         210  245        3560        .1  215  250        35   31         205  250        45     .025         195  209  0     14     .025         200  218  0     18     .05 200  215  0     15     .05 195  228  0     33     0.1 200  220  0     20     0.1 200  250  0     5061   2    .025         195  195  30    0     .025         200  200  30    0    100     .05 190  190  30    0     .05 180  180  30    0    100     0.1 165  165  30    0     0.1 220  220  30    0    100     .025         190  190  90    0     .025         197  197  90    0    100     .05 185  185  90    0     .05 200  200  90    0    100     0.1 185  185  90    0     0.1 205  205  90    0    100     .025         180  180  140   0     .025         200  200  150   0    100     .05 180  180  150   0     .05 195  195  150   0    100     0.1 185  185  150   0     0.1 180  194  150   14   80         180  200        20     .025         200  210  0     10     .025         215  225  0     10     .05 200  220  0     20     .05 215  235  0     20     0.1 210  235  0     25     0.1 215  245  0     3064   2    .025         195  195  30    0     .025         190  190  30    0    100     .05 195  195  30    0     .05 190  190  30    0    100     0.1 195  195  30    0     0.1 195  195  30    0    100     .025         185  185  90    0     .025         185  185  90    0    100     .05 190  190  90    0     .05 185  185  90    0    100     0.1 190  190  90    0     0.1 190  190  90    0    100     .025         190  190  150   0     .025         185  185  150   0    100     .05 185  185  150   0     .05 185  185  150   0    100     0.1 185  185  150   0     0.1 185  195  150   10   82         185  215        30         180  213        33     .05 220  255  0     35         210  250        40     .1  215  255        40         210  257        4764   5 P.O.*     .05 195  217  30    22   45         200  220        20     .1  200  225        25   32         200  235        35     .05 197  215  60    18   50         190  210        20     .1  195  225        30   25         195  230        35     .05 190  200  90    10   61         185  205        20     .1  185  210        25   36         190  220        30     .05 185  205  120   20   47         200  220        20     .1  185  210        25   41         200  227        27     .05 185  195  180   10   49         195  220        25     .1  190  207        17   41         195  230        35 oral dosage64        .05 190  200  240   10   49         195  220        25     .1  190  205        15   48         195  225        30     .025         215  245  0     30     .05 220  255        35     .1  220  255        3566   1    .025         207  225  15    18   40     .05 210  225        15   57     .1  215  230        15   57     .025         205  227  30    27   27     .05 215  230        15   57     .1  215  235        20   43     .025         210  230  60    20   33     .05 210  230        20   43     .1  210  240        30   1466   2    .025         195  205  90    10   67     .05 205  220        15   57     .1  205  210        5    86     .025         200  220  120   20   33     .05 200  215        15   57     .1  190  205        15   57     .025         180  195  150   15   50     .05 190  200        10   71     .1  185  205        20   43     .05 200  255  0     55     .1  200  255        5568   30   .05 180  190  30    10   82     .1  180  195        15   73     .05 170  185  90    15   73     .1  170  175        5    91     .05 185  205  150   20   64     .1  190  195        5    91__________________________________________________________________________ 
    
     Antihypertensive Effects in Conscious Aorta-Coarcted Renin/Angiotensin II-Dependent Renal Hypertensive Rats 
     Following the method reported by Chan et al., Drug Development Res. 18: 75-94, 1989, hypertension is induced by complete ligation of the aorta between the origin of the renal arteries according to the method of Rojo-Ortega and Genest (Can. J. Physio Pharmacol 46: 883-885, 1968.) and Fernandes et al., (J. Lab. Clin. Med. 87: 561-567, 1976) with modifications of the surgery procedures. Male Sprague-Dawley rats (Charles River Labs., Inc., Wilmington, Mass.) of 350 to 400 gm body weight are anesthetized with methohexital sodium (Brevital sodium, Eli Lilly and Co.) 60 mg/kg i.p. An incision is made in the left flank parallel to the rib cage. Using No. 3-0 silk suture(Davis &amp; Geck, Pearl River, NY), the aorta is completely ligated between the origins of the renal arteries. The wound is closed, and the animals returned to their individual cages. On the 7th day after aortic coarctation, the rats are used. The rats are restrained in a supine position with elastic tape, and the heads are immobilized by gentle restraining. The ventral portion of the neck is locally anesthetized by subcutaneous infiltration with 2% lidocaine. The left carotid artery is isolated and cannulated with a length of PE50 tubing, which is in turn, connected to a Statham P23Db pressure transducer - Beckman Dynagraph recording system. In some studies, the cannular is exteriorized through the back of the neck for long period of blood pressure monitoring. Recordings are taken over a 15-20 minute period, and the rats are dosed with the test compounds or vehicle (saline). After dosing, the blood pressure is monitored continuously. The results are shown in FIGS. 2 to 7. 
     When the compounds are employed for the above utility, they may be combined with one or more pharmaceutically acceptable carriers, for example, solvents, diluents and the like, and may be administered orally in such forms as tablets, capsules, dispersible powders, granules, or suspensions containing, for example, from about 0.05 to 5% of suspending agent, syrups containing, for example, from about 10 to 50% of sugar, and elixirs containing, for example, from about 20 to 50% ethanol, and the like, or parenterally in the form of sterile injectable solutions or suspension containing from about 0.05 to 5% suspending agent in an isotonic medium. Such pharmaceutical preparations may contain, for example, from about 0.05 up to about 90% of the active ingredient in combination with the carrier, more usually between about 5% and 60% by weight. 
     The effective dosage of active ingredient employed may vary depending on the particular compound employed, the mode of administration and the severity of the condition being treated. However, in general, satisfactory results are obtained when the compounds of the invention are administered at a daily dosage of from about 0.5 to about 500 mg/kg of animal body weight, preferably given in divided doses two to four times a day, or in sustained release form. For most large mammals the total daily dosage is from about 1 to 100 mg, preferably from about 2 to 80 mg. Dosage forms suitable for internal use comprise from about 0.5 to 500 mg of the active compound in intimate admixture with a solid or liquid pharmaceutically acceptable carrier. This dosage regimen may be adjusted to provide the optimal therapeutic response. for example, several divided doses may be administered daily or the dose may be proportionally reduced as indicated by the exigencies of the therapeutic situation. 
     These active compounds may be administered orally as well as by intravenous, intramuscular, or subcutaneous routes. Solid carriers include starch, lactose, dicalcium phosphate, microcrystalline cellulose, sucrose and kaolin, while liquid carriers include sterile water, polyethylene glycols, non-ionic surfactants and edible oils such as corn, peanut and sesame oils, as are appropriate to the nature of the active ingredient and the particular form of administration desired. Adjuvants customarily employed in the preparation of pharmaceutical compositions may be advantageously included, such as flavoring agents, coloring agents, preserving agents, and antioxidants, for example, vitamin E, ascorbic acid, BHT and BHA. 
     The preferred pharmaceutical compositions from the standpoint of ease of preparation and administration are solid compositions, particularly tablets and hard-filled or liquid-filled capsules. Oral administration of the compounds is preferred. 
     These active compounds may also be administered parenterally or intraperitoneally. Solutions or suspensions of these active compounds as a free base or pharmacologically acceptable salt can be prepared in water suitably mixed with a surfactant such as hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols and mixtures thereof in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms. 
     The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g., glycerol, propylene glycol and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils.