Abstract:
Broadly this invention provides inbred corn line ZS01591. The methods for producing a corn plant by crossing the inbred line ZS01591 are encompassed by the invention. Additionally, the invention relates to the various parts of inbred ZS01591 including culturable cells. This invention relates to hybrid corn seeds and plants produced by crossing the inbred line ZS01591 with at least one other corn line.

Description:
FIELD OF THE INVENTION 
     This invention is in the field of corn breeding, specifically relating to an inbred corn line designated ZS01591. 
     BACKGROUND OF THE INVENTION 
     The original maize plant was indigenous to the Western Hemisphere. The plants were weedlike and only through the efforts of early breeders was a cultivated crop species developed. The physical traits of maize are such that self pollination or cross pollination can occur. Each plant has a separate male and female flower, the tassel and ear, respectively. Natural pollination occurs when wind transfers pollen from tassel to the silks on the corn ears. This type of pollination contributed to the wide variation of maize varieties present in the Western Hemisphere. 
     The development of a planned breeding program for maize only occurred in the last century. Originally, maize was an open pollinated variety having heterogeneous genotypes. The maize farmer selected uniform ears from the yield of these genotypes and reserved them for planting the next season. The result was a field of maize plants that were segregating for a variety of traits. This type of maize selection lead to at most incremental increases in seed yield. 
     Large increases in seed yield were the result of the development of hybrid corn varieties in planned breeding programs. Hybrids were developed by selecting corn lines and selfing these lines for several generations to develop homozygous pure inbred lines and crossing selected inbred lines with unrelated inbred lines to produce hybrid progeny (F1). Inbred lines can be difficult to produce since the inbreeding process in corn decreases the vigor. However, when two inbred lines are crossed, the hybrid plant evidences greatly increased vigor compared to open pollinated segregating maize plants. An important factor of the homozygosity and the homogeneity of the inbred lines is that the hybrid from any cross will always be the same, and can be reproduced. 
     The ultimate objective of the commercial maize seed companies is to produce high yielding, agronomically sound plants which perform well in certain regions or areas of the Corn Belt. To produce these types of hybrids, the companies must develop inbreds which carry needed traits into the hybrid combination. Hybrids are not uniformly adapted for the Corn Belt, but are specifically adapted for regions of the Corn Belt. Northern regions of the Corn Belt require shorter season hybrids than do southern regions of the Corn Belt. Hybrids that grow well in Colorado and Nebraska soils may not flourish in rich Illinois soil. Thus, a variety of major agronomic traits are important in hybrid combination for the various Corn Belt regions, and have an impact on hybrid performance. 
     Inbred line development and hybrid testing have been emphasized in the past half century in commercial maize production as a means to increase hybrid performance. Inbred development is usually done by pedigree selection. Pedigree selection can be selection in an F 2  population produced from a planned cross of two genotypes (often elite inbred lines), or selection of progeny of synthetic varieties, open pollinated, composite, or backcross populations. This type of selection is effective for highly inheritable traits, but other traits, for example, yield requires replicated test crosses at a variety of stages for accurate selection. 
     Maize breeders select for a variety of traits in inbreds that impact hybrid performance along with selecting for acceptable parental traits. Such traits include yield potential in hybrid combination; dry down; maturity; grain moisture at harvest; greensnap; resistance to root lodging; resistance to stalk lodging; grain quality; disease and insect resistance; ear and plant height; performance in different soil types such as: low level of organic matter, clay, sand, black, high pH, low pH; performance in: wet environments, drought environments, and no tillage conditions. These traits appear to be governed by a complex genetic system that makes selection and breeding of an inbred line extremely difficult. Even if an inbred in hybrid combination has excellent yield (a desired characteristic), it may not be useful because it fails to have acceptable parental traits such as seed yield, seed size, pollen production, good silks, plant height, etc. 
     To illustrate the difficulty of breeding and developing inbred lines, the following example is given. Two inbreds compared for similarity of 29 traits differed significantly for 18 traits between the two lines. If 18 simply inherited single gene traits were polymorphic with gene frequencies of 0.5 in the parental lines, and assuming independent segregation (as would essentially be the case if each trait resided on a different chromosome arm), then the specific combination of these traits as embodied in an inbred would only be expected to become fixed at a rate of one in 262,144 possible homozygous genetic combinations. Selection of the specific inbred combination is also influenced by the specific selection environment on many of these 18 traits which makes the probability of obtaining this one inbred even more remote. Thus, the general procedure of producing a non segregating F 1  generation and self pollinating to produce a F 2  generation that segregates for traits does not easily lead to a useful inbred. Great care and breeder expertise must be used in selection of breeding material to continue to increase yield and agronomics of inbreds and resultant commercial hybrids. 
     SUMMARY OF THE INVENTION 
     The present invention relates to an inbred corn line ZS01591. Specifically, this invention relates to plants and seeds of this line. Additionally, this relates to a method of producing hybrid seed corn from this inbred. More particularly, this invention relates to the unique combination of traits that combine in corn line ZS01591. 
     Generally then, broadly the present invention includes an inbred corn seed designated ZS01591. This seed produces a corn plant. 
     The invention also includes the tissue culture of regenerable cells of ZS01591 wherein the tissue regenerates plants having the genotype of ZS01591. The tissue culture is selected from the group consisting of leaves, pollen, embryos, roots, root tips, anthers, silk, flowers, kernels, ears, cobs, husks and stalks, and cells and protoplasts thereof. The corn plant regenerated from ZS01591 having ZS01591&#39;s genotype. 
     The invention extends to hybrid seed produced by planting, in pollinating proximity, seeds of corn inbred lines ZS01591 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; and harvesting seeds produced on plants of the inbred. The hybrid seed produced by hybrid combination of plants of inbred corn seed designated ZS01591 and plants of another inbred line. Hybrid plants grown from this hybrid seed. 
     The invention further includes a method of hybrid F1 production. A first generation (F1) hybrid corn plant produced by the process of planting, in pollinating proximity, seeds of corn inbred lines ZS01591 and another inbred line; cultivating corn plants resulting from said planting; preventing pollen production by the plants of one of the inbred lines; allowing natural cross pollinating to occur between said inbred lines; harvesting seeds produced on plants of the inbred; and growing a harvested seed. 
     A tissue culture of the regenerable cells of hybrid plants produced with use of ZS01591 genetic material. A tissue culture of the regenerable cells of the corn plant produced by the method described above. 
     DEFINITIONS 
     In the description and examples which follow, a number of terms are used. In order to provide a clear and consistent understanding of the specifications and claims, including the scope to be given such terms, the following definitions are provided. 
     BL MOIST 
     The moisture percentage of the grain at black layer, i.e., when 50% of the plants per plot have reached physiological maturity. 
     COLD GERM 
     Cold Germ is a measurement of seed germination under cold soil conditions. Data is reported as percent of seed germinating. 
     ECB 
     European corn borer a maize eating insect. ECBI is the first brood generation of European corn borers. ECBII is the second generation of European corn borers. 
     EMERGE 
     The number of emerged plants per plot (planted at the same seedling rate) collected when plants have two fully developed leaves. 
     GI 
     This is a selection index which provides a single quantitative measure of the worth of a hybrid based on four traits. Yield is the primary trait contributing to index values. The GI value is calculated by combining stalk lodging, root lodging, yield and dropped ears according to the attached mathematical formula: 
     
         GI=100+0.5 (YLD)-0.9(% STALK LODGE)-0.9(% ROOT LODGE)-2.7(% DROPPED EAR) 
    
     GLS 
     Gray Leaf Spot (Cercospora Zeae) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     GW 
     Goss&#39; Wilt (Corynebacterium nebraskense). This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     HEATP10 
     The number of Growing Degree Units (GDU&#39;s) or heat units required for an inbred line or hybrid to have approximately 10 percent of the plants shedding pollen. This trait is measured from the time of planting. Growing Degree Units are calculated by the Barger Method where the GDU&#39;s for a 24 hour period are: 
     
         GDU=(Max Temp (°F.)+Min Temp (°F.))-50 
    
     The highest maximum temperature used is 86° F. and the lowest minimum temperature used is 50° F. For each inbred or hybrid it takes a certain number of GDU&#39;s to reach various stages of plant development. 
     HEATBL 
     The number of GDU&#39;s after planting when approximately 50 percent of the inbred or hybrid plants in a plot have grain which has reached physiological maturity (black layer). 
     HEATPEEK 
     The number of GDU&#39;s after planting of an inbred when approximately 50 percent of the plants show visible tassel extension. 
     HEATP50 or HTP50 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 50 percent of the plants shedding pollen. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATP90 
     The number of GDU&#39;s accumulated from planting when the last 100 percent of plants in an inbred or hybrid are still shedding enough viable pollen for pollination to occur. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS10 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 10 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS50 or HTS50 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 50 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     HEATS90 
     The number of GDU&#39;s required for an inbred or hybrid to have approximately 90 percent of the plants with silk emergence of at least 0.5 inches. Growing Degree Units are calculated by the Barger Method as shown in the HEATP10 definition. 
     MDMV A   
     Maize Dwarf Mosaic Virus strain A. The corn is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     MDMV B   
     Maize Dwarf Mosaic Virus strain B. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible and a &#34;9&#34; being very resistant.* 
     MOISTURE 
     The average percentage grain moisture of an inbred or hybrid at harvest time. 
     NLB 
     Northern Leaf Blight (Exserohilum turcicum) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     PCT TILLER 
     The total number of tillers per plot divided by the total number of plants per plot. 
     PLANT 
     This term includes plant cells, plant protoplasts, plant cell tissue cultures from which corn plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as embryos, pollen, flowers, kernels, ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk and the like. 
     PLANT HEIGHT 
     The distance in centimeters from ground level to the base of the tassel peduncle. 
     RM 
     Predicted relative maturity based on the moisture percentage of the grain at harvest. This rating is based on known set of checks and utilizes standard linear regression analyses and is referred to as the Minnesota Relative Maturity Rating System. 
     SHED 
     The volume of pollen shed by the male flower rated on a 1-9 scale where a &#34;1&#34; is a very light pollen shedder, a &#34;4.5&#34; is a moderate shedder, and a &#34;9&#34; is a very heavy shedder. If the Table(s) 3 have reduced the 1-9 shed scale to a 1-3 shed scale then any shed on Table 3 can be multiplied by 3 to reach the 1-9 shed scale. 
     SLB 
     Southern Leaf Blight (Bipolaris maydis) disease rating. This is rated on a 1-9 scale with a &#34;1&#34; being very susceptible, and a &#34;9&#34; being very resistant.* 
     TWT 
     The measure of the weight of grain in pounds for a one bushel volume adjusted for percent grain moisture. 
     VIGOR 
     Visual rating of 1 to 9 made 2-3 weeks post-emergence where a &#34;1&#34; indicates very poor early plant development, and a &#34;9&#34; indicates superior plant development. 
     WARM GERM 
     A measurement of seed germination under ideal (warm, moist) conditions. Data is reported as percent of seeds germinating. 
     YIELD (YLD) 
     Actual yield of grain at harvest adjusted to 15.5% moisture. Measurements are reported in bushels per acre. 
     % DROPPED EARS (DE) 
     The number of plants per plot which dropped their primary ear divided by the total number of plants per plot. 
     % LRG FLAT 
     Percentage by weight of shelled corn that passes through a 26/64 inch round screen and a 14/64 inch slot screen, but does not pass through a screen with 20.5/64 inch round openings. 
     % LRG ROUND 
     Percentage by weight of shelled corn that passes through a 26/64 inch round screen, but does not pass through a 14/64 inch slot screen or a screen with 20.5/64 inch round openings. 
     % MED FLAT 
     Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen and a 13/64 inch slotted screen, but does not pass through a screen with 17/64 inch round openings. 
     % MED ROUND 
     Percentage by weight of shelled corn that passes through a 20.5/64 inch round screen, but does not pass through a 13/64 inch slot screen or a screen with 17/64 inch round openings. 
     % SML FLAT 
     Percentage by weight of shelled corn that passes through a 17/64 inch round screen and a 12/64 inch slotted screen, but does not pass through a screen with 15/64 inch round openings. 
     % SML ROUND 
     Percentage by weight of shelled corn that passes through a 17/64 inch round screen, but does not pass through a 12/64 inch slotted screen or a screen with 15/64 inch round openings. 
     % ROOT LODGE (RL) 
     Percentage of plants per plot leaning more that 30 degrees from vertical divided by total plants per plot. 
     % STALK LODGE (SL) 
     Percentage of plants per plot with the stalk broken below the primary ear node divided by the total plants per plot. 
     * Resistant--on a scale of 1-9 with 9 evidencing the trait most strongly: 1-2.9 ratings are susceptible, 3-5.9 ratings are intermediate, and 6-9 ratings are resistant. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     ZS01591 can be used as a female or a male line due to its pollen shed and seed production abilities. This ZS01591 line evidences good general combining ability and specific combining ability with several types of males. 
     The inbred has shown uniformity and stability within the limits of environmental influence for all the traits as described in the Variety Description Information (Table 1) that follows. Most of the data in the Variety Description information was analyzed at Slater, Iowa. 
     The inbred has been self-pollinated for a sufficient number of generations to give inbred uniformity. During plant selection in each generation, the uniformity of plant type was selected to ensure homozygosity and phenotypic stability. The line has been increased in isolated farmland environments with data on uniformity and agronomic traits being observed to assure uniformity and stability. No variant traits have been observed or are expected in ZS01591. 
     The best method of producing the invention, ZS01591 which is substantially homozygous, is by planting the seed of ZS01591 which is substantially homozygous and self-pollinating or sib pollinating the resultant plant in an isolated environment, and harvesting the resultant seed or the resultant pollen. 
     
                                           TABLE 1__________________________________________________________________________ZS01591VARIETY DESCRIPTION INFORMATION#1 Type: Dent#2 Region Best Adapted: Southern and central corn belt regions,especiallythe east. 120 GRM.__________________________________________________________________________INBRED SV:ZS01591__________________________________________________________________________#3   MATURITYDAYS HEATUNITS 69   1570      FROM PLANTING TO 50% OF PLANTS IN SILK 67   1518      FROM PLANTING TO 50% OF PLANTS IN POLLEN           FROM 10% TO 90% POLLEN SHED__________________________________________________________________________#4   PLANTDATA  1             ANTHOCYANIN OF BRACE ROOTS: 1 = ABSENT 2 = FAINT 3 =           MODERATE 4 = DARK__________________________________________________________________________#5   LEAFCOLOR/DATA2/MEDIUM GREEN  LEAF COLOR **MUNSELL CODE-2.5GY 6/82               LEAF SHEATH PUBESCENCE (1 = NONE TO 9 = PEACH FUZZ)2               MARGINAL WAVES (1 = NONE TO 9 = MANY)2               LONGITUDINAL CREASES (1 = NONE TO 9 = MANY)__________________________________________________________________________#6   TASSELCOLOR/DATA           POLLEN SHED (0 = STERILE TO 9 = HEAVY SHEDDER)1/LIGHT GREEN   ANTHER COLOR **MUNSELL CODE-5Y 8/82/MEDIUM GREEN  GLUME COLOR **MUNSELL CODE-2.5GY 7/81               BAR GLUME: 1 = ABSENT 2 = PRESENT__________________________________________________________________________#7A  EAR (UNHUSKED DATA)COLOR/DATA 1/LIGHT GREEN  SILK COLOR (3 DAYS AFTER EMERGE) **MUNSELL CODE-2.5GY           8/6 1/LIGHT GREEN  FRESH HUSK (25 DAYS AFTER 50% SILK) **MUNSELL CODE-2.5GY           8/1022/TAN          DRY HUSK COLOR (65 DAYS AFTER 50% SILK **MUNSELL           CODE-2.5Y 8/4 3              POSITION OF EAR AT DRY HUSK: 1 = UPRIGHT 2 = HORIZONTAL 3           = PENDENT 6              HUSK TIGHTNESS (1 = VERY LOOSE TO 9 = VERY TIGHT) 2              HUSK EXTENSION AT HARVEST: 1 = EXPOSED EAR 2 = 8 CM 3 =           8-10 CM 4 = &gt;10 CM__________________________________________________________________________#7B  EAR (HUSKED DATA)DATA2               KERNEL ROWS: 1 = INDISTINCT 2 = DISTINCT1               ROW ALIGNMENT: 1 = STRAIT 2 = SLIGHT CURVE 3 = SPIRAL2               EAR TAPPER: 1 = STRAIT 2 = AVERAGE 3 = EXTREME__________________________________________________________________________#8   KERNEL (DRY)COLOR/DATA1               ALEURONE COLOR PATTERN: 1 = HOMO 2 = SEG7/YELLOW        ALEURONE COLOR **MUNSELL CODE-2.5Y 7/88/YELLOW-ORANGE HARD ENDOSPERM COLOR **MUNSELL CODE-2.5Y 7/83               ENDOSPERM TYPE6/PALE YELLOW   CROWN COLOR **MUNSELL CODE-2.5Y 8/10__________________________________________________________________________#9   COBCOLOR12/LIGHT RED    COB COLOR **MUNSELL CODE-5R 6/6__________________________________________________________________________1995 PROJECT SV PVP TRAITSINBRED ZS01591#10                    N   MEAN STD. T-STAT                                      PROB  95% CI__________________________________________________________________________EAR HEIGHT(CM)         15  66.60                           7.70 33.51 0.0000                                            (62.70, 70.50)LENGTH OF PRIMARY EAR LEAF (CM)                  15  87.80                           2.96 115.0 0.0000                                            (86.30, 89.30)WIDTH OF PRIMARY EAR LEAF (CM)                  15  9.07 0.70 49.90 0.0000                                            (8.71, 9.42)TOP EAR INTERNODE (CM) 15  11.33                           0.72 60.65 0.0000                                            (10.97, 11.70)DEGREE OF LEAF ANGLE   15  80.00                           0.00             (80.00, 80.00)# OF EARS PER PLANT    15  2.00 0.00             (2.00, 2.00)# OF LEAVES ABOVE TOP EAR                  15  6.27 0.46 53.02 0.0000                                            (6.04, 6.50)# OF PRIMARY LATERAL TASSEL BRANCHES                  15  6.00 0.93 25.10 0.0000                                            (5.53, 6.47)PLANT HEIGHT (CM)      15  154.5                           7.59 78.89 0.0000                                            (150.7, 158.4)TASSEL LENGTH (CM)     15  33.40                           2.80 46.23 0.0000                                            (31.98, 34.82)TASSEL BRANCH ANGLE    15  80.00                           0.00             (80.00, 80.00)# OF TILLER PER PLANTS 15  0.00 0.00             (0.00, 0.00)WEIGHT PER 100 KERNELS (GM)                  15  18.87                           2.26 32.28 0.0000                                            (17.72, 20.01)EAR LENGTH (CM)        15  17.83                           0.94 73.59 0.0000                                            (17.36, 18.31)EAR WEIGHT (GM)        15  85.67                           12.02                                27.60 0.0000                                            (79.58, 91.75)# OF KERNEL ROWS       15  14.27                           1.49 37.17 0.0000                                            (13.51, 15.02)COB DIAMETER AT MID-POINT (MM)                  14  24.93                           1.62 59.45 0.0000                                            (24.11, 25.76)EAR DIAMETER AT MID-POINT (MM)                  15  40.13                           1.60 97.29 0.0000                                            (39.32, 40.94)KERNEL LENGTH (MM)     15  9.80 0.68 56.14 0.0000                                            ( 9.46, 10.14)KERNEL THICKNESS (MM)  15  3.53 0.52 26.50 0.0000                                            (3.27, 3.79)KERNEL WIDTH (MM)      15  6.20 0.56 42.83 0.0000                                            (5.92, 6.48)% ROUND KERNELS (SHAPE GRADE)                  15  10.67                           1.76 23.48 0.0000                                            ( 9.78, 11.56)SHANK LENGTH           15  7.32 2.76 9.91  0.0000                                            (5.87,__________________________________________________________________________                                            8.77)#11 DISEASE RESISTANCE -          Northern leaf blight = 7.5          NLSr2* = 8.5          Gray leaf spot = 7          Eye = 4.5          GW = 6          MDMVB = 1* Northern Leaf Spot Race Two#12 Other comparable inbreds are B73, PVP#8300/48 and PVP#9000047__________________________________________________________________________ 
    
     The Munsell code is a reference book of color which is known and used in the industry and by persons with ordinary skill in the art of plant breeding. 
     The purity and homozygosity of inbred ZS01591 is constantly being tracked using isozyme genotypes as shown in Table 2. 
     Isozyme Genotypes for ZS01591 
     Isozyme data were generated for inbred corn line ZS01591 according to procedures known and published in the art, The data in Table 2 gives the electrophoresis data on ZS01591. 
     
                                           TABLE 2__________________________________________________________________________ELECTROPHORESIS RESULTS FOR ZS01591INBREDACP1    ACP4        ADH           MDH1               MDH2                   PGD1                       PGD2                           PH1                              PGM                                 IDH2__________________________________________________________________________ZS0159133  00  22 22  11  11  11  22 22 11Inbred and Hybrid Performance of ZS01591__________________________________________________________________________ 
    
     The traits and characteristics of inbred corn line ZS01591 are listed to compare with other inbreds and/or in hybrid combination. ZS01591 data shows the characteristics and traits of importance, giving a snapshot of ZS01591. 
     Table 3A shows a comparison between ZS01591 and B73. ZS01591 is very comparable to B73 although plant and ear height is lower. Flowering is similar except the mid-pollen shed is later for ZS01591 as shown by the HEATP50. Grain yield of ZS01591 is better and the grain moisture at harvest is less. Warm germination of the seed is significantly lower but still an acceptable level. 
     
                                           TABLE 3A__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                 PCT  PLANT                           EAR         EAR   PCTYEAR  INBRED      VIGOR EMERGE                 TILLER                      HEIGHT                           HEIGHT                                SHED   QUALITY                                             BARREN__________________________________________________________________________OVERALL ZS01591      6.5   74.2      170.8                           87.0 6.5 B73  6.1   86.7      180.0                           100  8.0 # EXPTS      4     4         4    4    4 DIFF 0.4   12.5      9.2  13.0 1.5 PROB 0.215 0.001*    0.200                           0.197                                0.103__________________________________________________________________________YEAR  INBRED      HEATP10            HEATP50                 HEATP90                      HEATS10                           HEATS50                                HEATS90__________________________________________________________________________OVERALL ZS01591      1564  1633 1706 1588 1623 1644 B73  1551  1591 1668 1592 1626 1661 # EXPTS      4     4    4    4    4    4 DIFF 13    41   38   4    3    17 PROB 0.186 0.066***                 0.287                      0.896                           0.924                                0.551__________________________________________________________________________                 BL   % ROOT                           % STALK                                % DROPPEDYEAR  INBRED      HEATPEEK            HEATBL                 MOIST                      LODGE                           LODGE                                EARS   MOISTURE                                             YIELD__________________________________________________________________________OVERALL ZS01591      1518  2575 27.0                  12.2  120.7 B73  1496  2652 29.5                  13.5  99.0 # EXPTS      4     1    1                     4     4 DIFF 22    77   2.5                   1.3   21.6 PROB 0.189                            0.106__________________________________________________________________________      WARM  COLD % LRG                      % LRG                           % MED                                % MED  % SML % SMLYEAR  INBRED      GERM  GERM ROUND                      FLAT ROUND                                FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01591      94.9  89.0 5.9  10.0 28.7 36.7   8.7   7.2 B73  97.9  89.1 5.5  6.0  28.2 43.0   7.0   8.8 # EXPTS      4     4    4    4    4    4      4     4 DIFF 3.0   0.1  0.5  4.0  0.5  6.3    1.6   1.7 PROB 0.023**            0.979                 0.797                      0.137                           0.870__________________________________________________________________________ 
    
     Table 3B compares ZS01591 with PVP#8300148. ZS01591 is comparable to PVP#8300148 for most inbred traits but has significantly lower seedling emergence scores. Maturity of ZS01591 is later than PVP#8300148 as evidenced by the significantly greater pollen and silk GDUs. Grain yield of ZS01591 is improved and grain moisture at harvest is similar. 
     
                                           TABLE 3B__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                   PCT  PLANT                             EAR         EAR   PCTYEAR  INBRED VIGOR EMERGE                   TILLER                        HEIGHT                             HEIGHT                                  SHED   QUALITY                                               BARREN__________________________________________________________________________OVERALL ZS01591        6.5   74.2      170.8                             87.0 6.5 PVP#8300148        7.2   89.2      172.7                             77.2 6.8 # EXPTS        4     4         4    4    4 DIFF   0.8   15.0      1.9  9.8  0.3 PROB   0.339 0.024**   0.822                             0.163                                  0.789__________________________________________________________________________YEAR  INBRED HEATP10              HEATP50                   HEATP90                        HEATS10                             HEATS50                                  HEATS90__________________________________________________________________________OVERALL ZS01591        1564  1633 1706 1588 1623 1644 PVP#8300148        1538  1577 1650 1519 1538 1577 # EXPTS        4     4    4    4    4    4 DIFF   26    56   57   69   85   67 PROB   0.074***              0.066***                   0.067***                        0.018**                             0.036**                                  0.023**__________________________________________________________________________                   BL   % ROOT                             % STALK                                  % DROPPEDYEAR  INBRED HEATPEEK              HEATBL                   MOIST                        LODGE                             LODGE                                  EARS   MOISTURE                                               YIELD__________________________________________________________________________OVERALL ZS01591        1518  2575 27.0                  12.2  120.7 PVP#8300148        1448  2630 28.0                  12.5  112.1 # EXPTS        4     1    1                     4     4 DIFF   71    55   1.0                   0.3   8.6 PROB   0.006*                           0.746__________________________________________________________________________        WARM  COLD % LRG                        % LRG                             % MED                                  % MED  % SML % SMLYEAR  INBRED GERM  GERM ROUND                        FLAT ROUND                                  FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01591        94.9  89.0 5.9  10.0 28.7 36.7   8.7   7.2 PVP#8300148        97.2  86.8 6.6  13.0 24.3 44.3   4.7   5.1 # EXPTS        4     4    4    4    4    4      4     4 DIFF   2.3   2.3  0.6  3.0  4.4  7.6    3.9   2.0 PROB   0.244 0.670                   0.634                        0.601                             0.502__________________________________________________________________________ 
    
     Table 3C is an inbred comparison between ZS01591 and PVP#9000047. ZS01591 has significantly greater plant and ear height than PVP#9000047. ZS01591 has similar silk maturity but sheds pollen significantly earlier GUDs for HEATP10 and HEATP90. Grain yield and harvest moisture are improved. Warm germination, seedling vigor and Shed of ZS01591 are significantly lower than PVP#9000047 but at acceptable levels. ZS01591 produces significantly less percent medium round kernels than PVP#9000047. 
     
                                           TABLE 3C__________________________________________________________________________PAIRED INBRED COMPARISON DATA__________________________________________________________________________                   PCT  PLANT                             EAR         EAR   PCTYEAR  INBRED VIGOR EMERGE                   TILLER                        HEIGHT                             HEIGHT                                  SHED   QUALITY                                               BARREN__________________________________________________________________________OVERALL ZS01591        6.2   80.4      173.6                             82.9 6.1 PVP#9000047        6.0   88.3      141.1                             62.0 6.9 # EXPTS        12    12        12   12   110 DIFF   0.2   8.0       32.5 20.9 0.8 PROB   0.389 0.006*    0.000*                             0.000*                                  0.022**__________________________________________________________________________YEAR  INBRED HEATP10              HEATP50                   HEATP90                        HEATS10                             HEATS50                                  HEATS90__________________________________________________________________________OVERALL ZS01591        1538  1603 1703 1557 1602 1645 PVP#9000047        1573  1617 1744 1580 1626 1681 # EXPTS        10    10   10   10   10   10 DIFF   35    14   41   23   24   36 PROB   0.019**              0.277                   0.034**                        0.150                             0.149                                  0.127__________________________________________________________________________                   BL   % ROOT                             % STALK                                  % DROPPEDYEAR  INBRED HEATPEEK              HEATBL                   MOIST                        LODGE                             LODGE                                  EARS   MOISTURE                                               YIELD__________________________________________________________________________OVERALL ZS01591        1477  2601 27.0                  14.7  84.6 PVP#9000047        1482  2669 29.0                  15.7  72.3 # EXPTS        10    2    1                     12    12 DIFF   5     68   2.0                   1.0   12.3 PROB   0.626 0.383                      0.458__________________________________________________________________________        WARM  COLD % LRG                        % LRG                             % MED                                  % MED  % SML % SMLYEAR  INBRED GERM  GERM ROUND                        FLAT ROUND                                  FLAT   ROUND FLAT__________________________________________________________________________OVERALL ZS01591        93.3  85.2 5.7  8.6  25.9 39.2   8.4   9.0 PVP#9000047        95.8  82.6 7.3  7.9  30.7 38.2   7.8   5.8 # EXPTS        9     9    11   11   11   11     11    11 DIFF   2.6   2.6  1.6  0.7  4.8  0.9    0.6   3.2 PROB   0.035**              0.563                   0.614                        0.745                             0.098***__________________________________________________________________________ 
    
     Table 4 shows the GCA (general combining ability) estimates of ZS01591 compared with the GCA estimates of the other inbreds. The estimates show the general combining ability is weighted by the number of experiment/location combinations in which the specific hybrid combination occurs. The interpretation of the data for all traits is that a positive comparison is a practical advantage. A negative comparison is a practical disadvantage. The general combining ability of an inbred is clearly evidenced by the results of the general combining ability estimates. This data compares the inbred parent in a number of hybrid combinations to a group of &#34;checks&#34;. The check data is from other companies&#39; hybrids, particularly the leader in the industry and ICI Seeds&#39; commercial products and pre-commercial hybrids which were grown in the same sets and locations. 
     Table 4A shows ZS01591 crossed to 50 different inbreds. ZS01591 in hybrid combination shows an advantage for yield by moisture of 0.4, a better rating for yield of 0.6 and a better advantage rating for moisture at 1.1. ZS01591 has tendency to have more stalk lodging and root lodging as indicated by the -0.9 and -0.6 disadvantage, respectively. 
     
                                           TABLE 4A__________________________________________________________________________ADVANTAGES OVER COMMERCIALS &amp; N.sup.1 S                                           ENT                                           ZS01591   N93 N94    N95       N  FI Y M                GI I YLD                        MST                           % SL                              % RL                                 % DE                                     TWT                                        POP                                           RM__________________________________________________________________________XR=   442 19 46 513          1.5             0.4                -1.0                   3.5                     0.6                        1.1                           -0.9                              -0.6                                 0.0 -0.8                                        85 118XH=         50 -0.4             0.2                -2.5                   2.7                     -2.2                        0.9                           -1.1                              -0.5                                 -0.0                                     -0.8                                        42 117XT=         3  3.7             0.5                2.0                   4.0                     6.4                        0.7                           -0.7                              -0.7                                 0.0 -1.2                                        379                                           119__________________________________________________________________________ XR = GCA ESTIMATE: WEIGHTED BY EXPT XH = GCA ESTIMATE: WEIGHTED BY PARENT2 XT = SAME AS XH, BUT USING ONLY THOSE PARENT2 WITH TWO YEARS OF DATA 
    
     Table 4B compares ZS01591 in random hybrid combinations with B73, PVP#8300148. ZS01591 shows a better yield/moisture advantage the B73, PVP#8300148. The yield advantage of ZS0159 is better than PVP#8300148, and the moisture advantage of ZS01591 is superior to this line. ZS01591 demonstrates a disadvantage in percent stalk and root lodging compared to PP#8300148 and is comparable to B73. 
     
                       TABLE 4B______________________________________          N       FI   YM   GI   I    YLD  MST______________________________________B73     XR=    82      -0.1 0.1  -0.1 0.7  2.0  0.0PVP     XR=    27394   -1.4 -0.2 0.4  -0.2 0.2  -0.8#8300148______________________________________           % SL    % RL % DE   TWT  POP  RM______________________________________B73     XR=     -1.0    -0.2 0.0    -0.6 305  124PVP8300148   XR=     0.1     0.2  0.0    0.2  -15  121______________________________________ 
    
     Table 5A shows the advantage the ZS01591 hybrid has over three commercially available ICI Seeds&#39; hybrids. The ZS01591 hybrid shows yield and moisture advantages over 8285 and 8281 with a slight disadvantage in stalk and root lodging. Compared to 8325, the ZS01591 hybrid shows an advantage for moisture and root lodging, and a disadvantage for yield and stalk lodging. 
     
                                           TABLE 5A__________________________________________________________________________HYBRID SUMMARYZS01591/INBREDCORN PAIRED COMPARISONSHY-BRID  TESTS     GI ADV           FI ADV                 YLD                    ADV                       MOIST                           ADV                              SL                                ADV                                   RL                                     ADV                                        DE                                          ADV                                             TWT                                                ADV     POP                                                ADV__________________________________________________________________________ZS  RE 19  161        -7 112              -5 138.5                    -6.5*                       21.5                           0.6                              5.2                                -3.6                                   3.3                                     0.2                                        0.0                                          0.0                                             49.7                                                -1.6                                                   24646                                                       183015198325    RE 19  168   117   145.0 22.1   1.6  3.5  0.0  51.3       24462    RE 19  161        -7 112              -5 138.5                    -6.5*                       21.5                           0.6                              5.2                                -3.6                                   3.3                                     0.2                                        0.0                                          0.0                                             49.7                                                -1.6    24646                                                183    RE 19  168   117   145.0 22.1   1.6  3.5  0.0  51.3       24462ZS  RE 10  179        6  130              9  167.5                    12.5*                       21.1                           1.5*                              3.5                                0.1                                   1.7                                     -0.4                                        0.0                                          0.0                                             50.3                                                0.2     24285                                                87015918285    RE 10  173   121   155.0 22.6   3.6  1.4  0.0  50.2       24198    RE 24  187        1  138              8  182.0                    5.7                       21.2                           3.2*                              2.4                                -1.5                                   2.1                                     1.1                                        0.1                                          -0.0                                             49.7                                                1.0     25365                                                121    RE 24  186   130   176.4 24.4   0.9  1.0  0.0  48.8       25244    RE 10  172        3  125              10 149.9                    7.7                       20.5                           3.1*                              3.4                                -0.7                                   0.2                                     -0.2                                        0.0                                          0.0                                             52.4                                                -1.5    23610                                                131    RE 10  169   115   142.2 23.5   2.7  0.0  0.0  54.0       23479    RE 44  182        3  133              8  171.4                    7.7*                       21.0                           2.8*                              2.9                                -0.9                                   1.6                                     -0.7                                        0.0                                          -0.0                                             50.7                                                0.0     24720                                                115    RE 44  179   125   163.7 23.8   1.9  0.9  0.0  50.7       24605ZS  RE 10  179        6  130              9  167.5                    11.4*                       21.1                           1.6*                              3.5                                0.3                                   1.7                                     -0.3                                        0.0                                          0.1                                             50.3                                                -0.2    24285                                                174015918281    RE 10  173   121   156.1 22.6   3.9  1.4  0.1  50.5       24110    RE 25  187        0  139              6  183.1                    0.9                       20.8                           2.5*                              2.7                                -1.0                                   2.3                                     1.0                                        0.1                                          -0.1                                             50.3                                                1.0     25709                                                358    RE 25  187   133   182.1 23.3   1.7  3.3  0.0  49.2       25351    RE 25  164        -6 116              -4 142.4                    -3.3                       20.8                           1.0*                              5.12.4 2.6 -2.3     0.0        -0.0           50.2              -1.8                 24550                    -70    RE 25  170   120   145.7 21.8   2.8  0.3  0.0  52.0       24620    RE 60  176        -1 128              2  163.5                    0.9                       20.8                           1.7*                              3.91.4 2.3 -0.6     0.1        -0.0           50.3              -0.7                 24989                    149    RE 60  177   126   162.6 22.6   2.5  1.7  0.0  50.9       24840__________________________________________________________________________ 
    
     Table 6A shows the entomology strengths and weaknesses of the inbred. 
     
                       TABLE 6A______________________________________                       EAR     PLANT   ECB1      ECB2      DAMAGE  INTEGRITYINBRED  RATING    RATING    RATING  RATING______________________________________ZS01591 3         5.7       2.8     6______________________________________ 
    
     The inbred ZS01591 can be employed as the female or the male plant in a hybrid production field. This inbred is a medium tall line with moderately high ear height. ZS01591 has excellent female seed yield. The inbred has good plant health and tolerance to gray leaf spot (GLS) disease, which is of value in the eastern corn regions. 
     In hybrid combination, ZS01591 has top-end yield potential in high yield environments. This inbred has good general combining and specific combining ability for yield with several male lines. In addition to its yield potential, it has a good moisture advantage compared to other hybrids adapted to its same growing region, resulting in positive yield/moisture advantages. The tolerance of ZS01591 to GLS is carried into its hybrids which is of value in the eastern growing regions. 
     The foregoing is set forth by way of example and is not intended to limit the scope of the invention. 
     This invention also is directed to methods for producing a corn plant by crossing a first parent corn plant with a second parent corn plant wherein the first or second parent corn plant is an inbred corn plant from the line ZS01591. Further, both first and second parent corn plants can come from the inbred corn line ZS01591. A variety of breeding methods can be selected depending on the mode of reproduction, the trait, the condition of the germplasm. Thus, any such methods using the inbred corn line ZS01591 are part of this invention: selfing, backcrosses, hybrid production, crosses to populations, haploid and anther culturing and the like. 
     Various culturing techniques known to those skilled in the art, such as haploid, transformation, and a host of other conventional and unconventional methods are within the scope of the invention. All plants and plant cells produced using inbred corn line ZS01591 are within the scope of this invention. The invention encompasses the inbred corn line used in crosses with other, different, corn inbreds to produce (F1) corn hybrid seeds and plants with the characteristics that make good hybrids. This invention includes cells which upon growth and differentiation produce corn plants having the physiological and morphological /moisture advantage than B73, PUP# 8300148. The yield advantage of ZS01591 characteristics of the inbred line ZS01591. 
     Duncan, from at least 1985-1988 produced literature on plant regeneration from callus. Both inbred and hybrid callus have resulted in regenerated plants at excellent efficiency rates. Somatic embryogenesis has been performed on various maize tissue such as glume which before the 1980&#39;s was considered unusable for this purpose. The prior art clearly teaches the regeneration of plants from various maize tissues. 
     European Patent Application, publication 160,390, describes tissue culture of corn which can be used by those skilled in the art. Corn tissue culture procedures are also described in the literature as early as 1982. 
     A deposit of at least 2500 seeds of the inbred seed of this invention is maintained by ICI Seeds, 2369 330th Street, Slater, Iowa 50244. Access to this deposit will be available during the pendency of this application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. The Applicant made a deposit on Jun. 11, 1997, of at least 2500 seeds of Inbred Corn Line ZS01591 with the American Type Culture Collection (ATCC), located at 12301 Parklawn Drive, Rockville, Md. 20852. The ATCC accession number is 209115. Additionally the Applicant has satisfied all of the requirements of C.F.R.§1.801-1.809, including providing an indication of the viability of the sample. The ATCC deposit will be maintained in that depository, which is a public depository, for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. 
     Inbreds designated MBS are available from Mike Brayton Seed in Iowa. Inbreds designated SGI are available from Seed Genetic Inc. in New Jersey. Information on some ZS designations may be available from the PVP office. 
     Accordingly, the present invention has been described with some degree of particularity directed to the preferred embodiment of the present invention. It should be appreciated, though, that the present invention is defined by the following claims construed in light of the prior art so that modifications or changes may be made to the preferred embodiment of the present invention without departing from the inventive concepts contained herein.