Abstract:
A series of genes form  Neisseria meningitidis  are shown to encode products which are responsible for DNA uptake. The identification of these genes therefore allows attenuated microorganisms to be produced that have a reduced ability to take up DNA. Microorganisms of the invention may be used in the production of genetically stable mutant microorganisms. The genes or their encoded products can be used in the manufacture of vaccines for therapeutic application.

Description:
FIELD OF THE INVENTION  
         [0001]    This invention relates to bacterial genes and proteins, and their use. More particularly, it relates to genes and proteins/peptides obtained from  Neisseria meningitidis,  and their use in therapy and in screening for drugs.  
         BACKGROUND OF THE INVENTION  
         [0002]    [0002] Neisseria meningitidis  is a Gram-negative bacterial pathogen that is implicated in septic shock and bacterial meningitis. This bacterium is a leading cause of bacterial meningitis in developed countries, and causes large-scale epidemics in Africa and China. In the UK,  Neisseria meningitidis  is the leading cause of death in childhood apart from road traffic accidents. The bacterium naturally inhabits the human naso-pharynx and then gains access to the blood stream from where it causes severe septicaemia or meningitis. Although current anti-microbials are effective in eliminating the bacterium from the body, the mortalilty from menigococcal septicaemia remains substantial.  
           [0003]    Immunisation with avirulent  N. meningitidis  microorganisms has also been proposed. Avirulence is achieved through genetic modification to disrupt the expression of genes implicated in virulence. One concern is that a mutant strain may revert to its virulent form by taking up replacement DNA. It is therefore desirable to provide means for treating or preventing conditions caused by  Neisseria meningitidis,  e.g. by immunisation.  
         SUMMARY OF THE INVENTION  
         [0004]    The present invention is based on the discovery of genes in  Neisseria meningitidis  that are required for natural competence (uptake and incorporation of exogenous DNA).  
           [0005]    The genes identified herein may be utilised to prepare microorganisms that are transformation-deficient. The mutant microorganisms will usually have a mutation that disrupts the expression of one or more of the genes identified herein, to provide a strain that lacks the ability to uptake DNA. These microorganisms will also have use in therapy and diagnosis.  
           [0006]    According to a first aspect of the invention, a microorganism comprises a mutation that disrupts the expression of a gene comprising any of the nucleotide sequences identified in claim 1, or a homologue thereof with at least 40% sequence identity, for therapeutic or diagnostic use.  
           [0007]    The microorganisms may have many therapeutic uses for treating Neisseria infections, including use in vaccines for prophylactic application.  
           [0008]    According to a second aspect, a peptide is encoded by an operon including any of the nucleotide sequences identified in claim 1. 
       
    
    
     DESCRIPTION OF THE INVENTION  
       [0009]    The present invention is based on the discovery of genes encoding peptides which are responsible for the uptake of DNA. The peptides and genes of the invention are therefore useful for the preparation of therapeutic agents to treat infection. It should be understood that references to therapy also include preventative treatments, e.g. vaccination. Furthermore, while the products of the invention are intended primarily for treatment of infections in human patients, veterinary applications are also considered to be within the scope of the invention.  
         [0010]    The present invention is described with reference to  Neisseria meningitidis.  However, all the Neisseria strains, and many other Gram-negative bacterial strains are likely to include related peptides or proteins having amino acid sequence identity or similarity to those identified herein. Organisms likely to contain the peptides include, but are not limited to the genera Salmonella, Enterobacter, Klebsiella, Shigella and Yersinia.  
         [0011]    Preferably, the peptides that may be useful in the various aspects of the invention have greater than a 40% similarity with the peptides identified herein. More preferably, the peptides have greater than 60% sequence similarity. Most preferably, the peptides have greater than 80% sequence similarity, e.g. 95% similarity. With regard to the polynucleotide sequences identified herein, related polynucleotides that may be useful in the various aspects of the invention may have greater than 40% identity with the sequences identified herein. More preferably, the polynucleotide sequences have greater than 60% sequence identity. Most preferably, the polynucleotide sequences have greater than 80% sequence identity, e.g. 95% identity.  
         [0012]    The terms “similarity” and “identity” are known in the art. The use of the term “identity” refers to a sequence comparison based on identical matches between correspondingly identical positions in the sequences being compared. The term “similarity” refers to a comparison between amino acid sequences, and takes into account not only identical amino acids in corresponding positions, but also functionally similar amino acids in corresponding positions. Thus similarity between polypeptide sequences indicates functional similarity, in addition to sequence similarity.  
         [0013]    Levels of identity between gene sequences and levels of identity or similarity between amino acid sequences can be calculated using known methods. In relation to the present invention, publicly available computer based methods for determining identity and similarity include the BLASTP, BLASTN and FASTA (Atschul et al., J. Molec. Biol., 1990; 215:403-410), the BLASTX program available from NCBI, and the Gap program from Genetics Computer Group, Madison Wis. The levels of similarity and identity provided herein, were obtained using the Gap program, with a Gap penalty of 12 and a Gap length penalty of 4 for determining the amino acid sequence comparisons, and a Gap penalty of 50 and a Gap length penalty of 3 for the polynucleotide sequence comparisons.  
         [0014]    Having characterised a gene according to the invention, it is possible to use the gene sequence to search for related genes or peptides in other microorganisms. This may be carried out by searching in existing databases, e.g. EMBL or GeneBank.  
         [0015]    Peptides or proteins according to the invention may be purified and isolated by methods known in the art. In particular, having identified the gene sequence, it will be possible to use recombinant techniques to express the genes in a suitable host. Active fragments and related molecules can be identified and may be useful in therapy. For example, the peptides or their active fragments may be used as antigenic determinants in a vaccine, to elicit an immune response. They may also be used in the preparation of antibodies, for passive immunisation, or diagnostic applications. Suitable antibodies include monoclonal antibodies, or fragments thereof, including single chain Fv fragments. Methods for the preparation of antibodies will be apparent to those skilled in the art.  
         [0016]    Active fragments of the peptides are those that retain the biological function of the peptide, i.e. retain the ability to take up DNA. Typically, the fragment will be at least 30 nucleotides (10 amino acids) in size, preferably 60 nucleotides (20 amino acids) and most preferably greater than 90 nucleotides (30 amino acids) in size.  
         [0017]    The genes identified herein are likely to be useful both in generating vaccine strains that cannot take up exogenous DNA, and as a target for antimicrobials. Attenuated microorganisms can be produced with an attenuating mutation in a virulence gene or genes together with a mutation that disrupts a gene or genes of the invention, providing an attenuated microorganism that cannot take up extracellular DNA.  
         [0018]    The term “virulence” is known in the art and refers to the pathogenicity of a microorganism. The use of the term “avirulent” is intended therefore to refer to microorganisms that are no longer pathogenic.  
         [0019]    Suitable virulence genes will be apparent to the skilled person, and include auxotroph genes and those identified in Sun et al., Nature medicine, 2000; 6(11): 1269-1273.  
         [0020]    The skilled person will be aware of methods for disrupting the expression of particular genes. Techniques that may be used include insertional inactivation or gene deletion techniques. Attenuated microorganisms according to the invention may also comprise additional mutations in other genes.  
         [0021]    The preparation of vaccines based on attenuated microorganisms is known to those skilled in the art. Vaccine compositions can be formulated with suitable carriers or adjuvants, e.g. alum, as necessary or desired, to provide effective immunisation against infection. The preparation of vaccine formulations will be apparent to the skilled person.  
         [0022]    Attenuated microorganisms may also be used as carrier systems for the delivery of heterologous antigens, therapeutic proteins or nucleic acids (DNA or RNA). In this embodiment, the attenuated microorganisms are used to deliver a heterologous antigen, protein or nucleic acid to a particular site in vivo. Introduction of a heterologous antigen, peptide or nucleic acid into an attenuated microorganism can be carried out by conventional techniques, including the use of recombinant constructs, e.g. vectors, which comprise polynucleotides that express the heterologous antigen or therapeutic protein, and also include suitable promoter sequences. As the microorganisms are transformation-deficient, it will be necessary to incorporate heterologous nucleic acids via an active mechanism, e.g. electroporation. This is a known technique. Alternatively, the gene that encodes the heterologous antigen or protein may be incorporated into the genome of the organism and the endogenous promoters used to control expression. A further alternative is to modify the microorganisms to disrupt the genes identified herein after first transforming with the heterologous gene.  
         [0023]    More generally, and as is well known to those skilled in the art, a suitable amount of an active component of the invention can be selected, for therapeutic use, as can suitable carriers or excipients, and routes of administration. These factors would be chosen or determined according to known criteria such as the nature/severity of the condition to be treated, the type and/or health of the subject etc.  
         [0024]    The various products of the invention may also be used in veterinary applications.  
         [0025]    The following is a brief overview of the experimental procedure used to identify the genes.  
         [0026]    Signature-tagged mutagenesis (STM) (Hensel et al., Science, 1995; 269: 400-403) was used to identify the genes in  N. meningitidis  that are essential for uptake of DNA. In STM, individual mutants are tagged with unique sequence identifiers, allowing large numbers of mutants to be analysed simultaneously.  
         [0027]    A  Neisseria meningitidis  serogroup B Tn10 mutant bank was used to screen for mutants that exhibited a transformation defect. Pools of mutants were transformed with either plasmid or chromosomal DNA carrying an erythromycin-resistance marker. Transformation-deficient mutants were identified by plating out transformed pools of mutant bacteria onto solid media, in the presence and absence of erythromycin and performing a STM screen. Bacteria containing a modified version of the transposon Tn10 inserted within a competence gene failed to be recovered from plates containing erythromycin inoculated with a mixed population of mutants, and were, therefore likely to be defective in the uptake of exogenous DNA.  
         [0028]    The cloned  Neisseria meningitidis  nucleotide sequences immediately following the Tn10 insertion were identified, and their translated sequences characterised. Homologues were then searched for in existing protein and DNA databases (www.sanger.ac.uk/Projects/N-meningitidis; www.tigr.org; and www.ncbi.nlm.nih.gov).  
         [0029]    The selected mutants were tested for the ability to take up exogenous DNA and were shown to take up exogenous DNA at levels at least 5 fold below wild-type levels. As the genes are transcribed as part of an operon it is possible that the mutation is due to a polar effect on a downstream gene.  
         [0030]    To prevent the identification of mutants that merely could not express an erythromycin resistant or capsule-minus phenotype, all putatively competence-defective mutants were also analysed for their ability to be transformed to tetracycline resistance. The results confirmed that the mutants were transformation deficient.  
         [0031]    The genes of the invention are shown in Table 1 together with the identification of any known homologue. In addition, Table 1 shows the transformation frequency, illustrating the deficiency of DNA uptake exhibited by the mutants, compared to wild-type.  
                               TABLE 1                       SEQ           Transformation   Accession       ID NO.   Genes   Function   frequency   number                    1   recG   ATP-dependent DNA helicase RECG   1/5   NMB1788        3   rho   Transcription termination factor RHO   0   NMB0617        5   pilN   PBP1   0   NMB1809        7   phlI   Phosphoenolpyruvate-protein    1/30   NMB2044               phosphotransferase        9   cutE     E. coli  apolipoprotein N-acyltransferase    1/10   NMB0713       11   smf   SMF protein (DNA processing chain A)   0   NMB0116       13       CcsA-related protein     1/2000   NMB0296       15   vacB     E. coli  Vacb protein    1/10   NMB1200       17       No hit in NM nucleic acid database   1/5       19   proC   Pyrroline-5-carboxylate reductase   0   NMB055       21       Phorphoribosylglycinamide    1/40   NMB1566               formyltransferase                  
 
         [0032]    [0032] 
     
       
       
         1 
         
           
             22  
           
           
             1  
             2040  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(2040)  
             
           
            1 

atg atg tcg ccc gaa acc cga aaa cag ctc aaa atc acc gat gtt tcc       48 
Met Met Ser Pro Glu Thr Arg Lys Gln Leu Lys Ile Thr Asp Val Ser 
  1               5                  10                  15 

gcc aag aag ctc gac aaa ctc aac ctc cat acc gcg tgg gat ttg gtg       96 
Ala Lys Lys Leu Asp Lys Leu Asn Leu His Thr Ala Trp Asp Leu Val 
             20                  25                  30 

ttg cac ctg ccg ctg cgt tac gag gac gag acg cac att atg ccg att      144 
Leu His Leu Pro Leu Arg Tyr Glu Asp Glu Thr His Ile Met Pro Ile 
         35                  40                  45 

aag gac gcg ccg att ggc gtg ccg tgt cag gtc gag ggg gag gtt atc      192 
Lys Asp Ala Pro Ile Gly Val Pro Cys Gln Val Glu Gly Glu Val Ile 
     50                  55                  60 

cat cag gaa gta acg ttc aaa ccg cgc aag cag ctg att gtc caa atc      240 
His Gln Glu Val Thr Phe Lys Pro Arg Lys Gln Leu Ile Val Gln Ile 
 65                  70                  75                  80 

gcc gac ggc tcc ggc agc gtc ctt ttt ctg cgc ttc atc cac ttt tac      288 
Ala Asp Gly Ser Gly Ser Val Leu Phe Leu Arg Phe Ile His Phe Tyr 
                 85                  90                  95 

gcc agc cat cag aaa cag acg gcg atc ggc aaa cgc atc cgc gcc gtg      336 
Ala Ser His Gln Lys Gln Thr Ala Ile Gly Lys Arg Ile Arg Ala Val 
            100                 105                 110 

ggc gaa atc aaa cac ggc ttt tac ggc gac gag atg att cat ccc aaa      384 
Gly Glu Ile Lys His Gly Phe Tyr Gly Asp Glu Met Ile His Pro Lys 
        115                 120                 125 

atc cgc gat gcc gag ggc ggc ggt ttg gcg gaa agc ctc acg ccg gtt      432 
Ile Arg Asp Ala Glu Gly Gly Gly Leu Ala Glu Ser Leu Thr Pro Val 
    130                 135                 140 

tac ccg acc gta aac ggt ttg aac cag ccc act ttg cgc cgt att att      480 
Tyr Pro Thr Val Asn Gly Leu Asn Gln Pro Thr Leu Arg Arg Ile Ile 
145                 150                 155                 160 

cag acg gcg ttg gac gtt acg ccg ctg cac gac acg ctg ccc gat gcc      528 
Gln Thr Ala Leu Asp Val Thr Pro Leu His Asp Thr Leu Pro Asp Ala 
                165                 170                 175 

ctg ctg tgc cgt ctg aag ctg cca cac ctc gcc gaa agc ctg cgt ctt      576 
Leu Leu Cys Arg Leu Lys Leu Pro His Leu Ala Glu Ser Leu Arg Leu 
            180                 185                 190 

ttg cat tcg ccg ccg ccg agt ttc acg att cat cag ctt tca gac ggc      624 
Leu His Ser Pro Pro Pro Ser Phe Thr Ile His Gln Leu Ser Asp Gly 
        195                 200                 205 

acg ctg cct gca tgg caa cgg ctc aaa ttc gac gaa ctt ttg gcg caa      672 
Thr Leu Pro Ala Trp Gln Arg Leu Lys Phe Asp Glu Leu Leu Ala Gln 
    210                 215                 220 

cag ctt tcg atg cgc ttg gcg cga cag aag cgt atc ggc ggc acg gcg      720 
Gln Leu Ser Met Arg Leu Ala Arg Gln Lys Arg Ile Gly Gly Thr Ala 
225                 230                 235                 240 

gcg gca ttg ggc ggc gac ggc aca ttg acc caa gcc ctg cgc caa gcc      768 
Ala Ala Leu Gly Gly Asp Gly Thr Leu Thr Gln Ala Leu Arg Gln Ala 
                245                 250                 255 

ctg ccg ttt gcc ctg acc gat gcg caa gaa aaa gtt gtt tcc gaa atc      816 
Leu Pro Phe Ala Leu Thr Asp Ala Gln Glu Lys Val Val Ser Glu Ile 
            260                 265                 270 

tgc cgc gat atg gcg caa acc tac ccg atg cac cgc ctg ctg cag ggc      864 
Cys Arg Asp Met Ala Gln Thr Tyr Pro Met His Arg Leu Leu Gln Gly 
        275                 280                 285 

gat gtc ggc agc ggc aaa acc att gtg gct gct ttg tct gct ttg acg      912 
Asp Val Gly Ser Gly Lys Thr Ile Val Ala Ala Leu Ser Ala Leu Thr 
    290                 295                 300 

gct atc gaa tcc ggt gcg cag gtg gct gta atg gcg ccc act gaa atc      960 
Ala Ile Glu Ser Gly Ala Gln Val Ala Val Met Ala Pro Thr Glu Ile 
305                 310                 315                 320 

ctt gcc gaa cag cat ttt att aaa ttt aaa caa tgg ctc gaa cct ttg     1008 
Leu Ala Glu Gln His Phe Ile Lys Phe Lys Gln Trp Leu Glu Pro Leu 
                325                 330                 335 

ggc att gaa gtt gtc cgc ctt ttt ggc agt ttg cgt aaa aaa gcc aaa     1056 
Gly Ile Glu Val Val Arg Leu Phe Gly Ser Leu Arg Lys Lys Ala Lys 
            340                 345                 350 

gac gaa gcc aaa gcc aaa ctg gct gac ggc agc gtc aaa atc gcc gtc     1104 
Asp Glu Ala Lys Ala Lys Leu Ala Asp Gly Ser Val Lys Ile Ala Val 
        355                 360                 365 

ggc acg cac gcc ctg ttt tca gac ggc gtg gcg ttt cac aat ttg ggc     1152 
Gly Thr His Ala Leu Phe Ser Asp Gly Val Ala Phe His Asn Leu Gly 
    370                 375                 380 

ttg acc att gtg gac gaa cag cac cgt ttc ggc gtt gcc caa cgc ctc     1200 
Leu Thr Ile Val Asp Glu Gln His Arg Phe Gly Val Ala Gln Arg Leu 
385                 390                 395                 400 

gcc ctc aaa aac aaa ggg cgc gaa gtc cat cag ctg atg atg tcc gcc     1248 
Ala Leu Lys Asn Lys Gly Arg Glu Val His Gln Leu Met Met Ser Ala 
                405                 410                 415 

acg ccc atc ccg cgc acg ctt gcg atg agt ttt ttc gcc gat ttg gac     1296 
Thr Pro Ile Pro Arg Thr Leu Ala Met Ser Phe Phe Ala Asp Leu Asp 
            420                 425                 430 

gtg tcc gtc atc gac gaa ttg ccg ccc ggg cgc aca ccg att aaa acg     1344 
Val Ser Val Ile Asp Glu Leu Pro Pro Gly Arg Thr Pro Ile Lys Thr 
        435                 440                 445 

cgc ctc gtc aac aac gtc cgc cgc gcc gaa gtc gaa ggc ttc gtc ctc     1392 
Arg Leu Val Asn Asn Val Arg Arg Ala Glu Val Glu Gly Phe Val Leu 
    450                 455                 460 

ggc act tgc cga aaa ggg cgg cag gcg tat tgg gtc tgc cca ttg att     1440 
Gly Thr Cys Arg Lys Gly Arg Gln Ala Tyr Trp Val Cys Pro Leu Ile 
465                 470                 475                 480 

gaa gaa agc gaa acc ctg caa ctg caa acc gcc gcc gaa acc ctc gcc     1488 
Glu Glu Ser Glu Thr Leu Gln Leu Gln Thr Ala Ala Glu Thr Leu Ala 
                485                 490                 495 

cgg ctt cag acg gca ttg ccc gaa ctc aat atc gga ctg gta cac ggg     1536 
Arg Leu Gln Thr Ala Leu Pro Glu Leu Asn Ile Gly Leu Val His Gly 
            500                 505                 510 

cgc atg aag gcc gcc gaa aag gcc gaa gtg atg gcg cgg ttt tct tcg     1584 
Arg Met Lys Ala Ala Glu Lys Ala Glu Val Met Ala Arg Phe Ser Ser 
        515                 520                 525 

ggg ggg ctg aac gtc ttg gtc gcc acc acc gtc atc gaa gtc ggc gta     1632 
Gly Gly Leu Asn Val Leu Val Ala Thr Thr Val Ile Glu Val Gly Val 
    530                 535                 540 

gat gtg ccc aat gcc gcc ctg atg gtc atc gaa cac gcc gag cgc atg     1680 
Asp Val Pro Asn Ala Ala Leu Met Val Ile Glu His Ala Glu Arg Met 
545                 550                 555                 560 

ggc ttg gcg cag ctt cac caa tta cgc gga cgg gta ggg cgc ggc gcg     1728 
Gly Leu Ala Gln Leu His Gln Leu Arg Gly Arg Val Gly Arg Gly Ala 
                565                 570                 575 

gcg gaa agc gtg tgc gtc ctc ctg ttt gcc gaa ccc ttg ggc gaa ctc     1776 
Ala Glu Ser Val Cys Val Leu Leu Phe Ala Glu Pro Leu Gly Glu Leu 
            580                 585                 590 

gcc aaa gcg cgg ctg aaa gtc atc tac gaa cac acc gac ggc ttc gaa     1824 
Ala Lys Ala Arg Leu Lys Val Ile Tyr Glu His Thr Asp Gly Phe Glu 
        595                 600                 605 

atc gcc cgc caa gac ctc aat atc cgc ggc ccg ggc gag ttt ctc ggc     1872 
Ile Ala Arg Gln Asp Leu Asn Ile Arg Gly Pro Gly Glu Phe Leu Gly 
    610                 615                 620 

gcg cgc caa agc ggc gtg cct atg ctg cgt ttc gcc aaa ctc gaa gaa     1920 
Ala Arg Gln Ser Gly Val Pro Met Leu Arg Phe Ala Lys Leu Glu Glu 
625                 630                 635                 640 

gac ctg cac ctt ttg gaa cag gcg cgc gaa acc gcc ccg atg ctg att     1968 
Asp Leu His Leu Leu Glu Gln Ala Arg Glu Thr Ala Pro Met Leu Ile 
                645                 650                 655 

gag caa aac ccc gaa atc gtc gaa gcg cat ttg gca agg tgg ctt tcc     2016 
Glu Gln Asn Pro Glu Ile Val Glu Ala His Leu Ala Arg Trp Leu Ser 
            660                 665                 670 

ggc agg gaa ggg tat ttg ggc gtg                                     2040 
Gly Arg Glu Gly Tyr Leu Gly Val 
        675                 680 

 
           
             2  
             680  
             PRT  
             Neisseria meningitidis  
           
            2 

Met Met Ser Pro Glu Thr Arg Lys Gln Leu Lys Ile Thr Asp Val Ser 
  1               5                  10                  15 

Ala Lys Lys Leu Asp Lys Leu Asn Leu His Thr Ala Trp Asp Leu Val 
             20                  25                  30 

Leu His Leu Pro Leu Arg Tyr Glu Asp Glu Thr His Ile Met Pro Ile 
         35                  40                  45 

Lys Asp Ala Pro Ile Gly Val Pro Cys Gln Val Glu Gly Glu Val Ile 
     50                  55                  60 

His Gln Glu Val Thr Phe Lys Pro Arg Lys Gln Leu Ile Val Gln Ile 
 65                  70                  75                  80 

Ala Asp Gly Ser Gly Ser Val Leu Phe Leu Arg Phe Ile His Phe Tyr 
                 85                  90                  95 

Ala Ser His Gln Lys Gln Thr Ala Ile Gly Lys Arg Ile Arg Ala Val 
            100                 105                 110 

Gly Glu Ile Lys His Gly Phe Tyr Gly Asp Glu Met Ile His Pro Lys 
        115                 120                 125 

Ile Arg Asp Ala Glu Gly Gly Gly Leu Ala Glu Ser Leu Thr Pro Val 
    130                 135                 140 

Tyr Pro Thr Val Asn Gly Leu Asn Gln Pro Thr Leu Arg Arg Ile Ile 
145                 150                 155                 160 

Gln Thr Ala Leu Asp Val Thr Pro Leu His Asp Thr Leu Pro Asp Ala 
                165                 170                 175 

Leu Leu Cys Arg Leu Lys Leu Pro His Leu Ala Glu Ser Leu Arg Leu 
            180                 185                 190 

Leu His Ser Pro Pro Pro Ser Phe Thr Ile His Gln Leu Ser Asp Gly 
        195                 200                 205 

Thr Leu Pro Ala Trp Gln Arg Leu Lys Phe Asp Glu Leu Leu Ala Gln 
    210                 215                 220 

Gln Leu Ser Met Arg Leu Ala Arg Gln Lys Arg Ile Gly Gly Thr Ala 
225                 230                 235                 240 

Ala Ala Leu Gly Gly Asp Gly Thr Leu Thr Gln Ala Leu Arg Gln Ala 
                245                 250                 255 

Leu Pro Phe Ala Leu Thr Asp Ala Gln Glu Lys Val Val Ser Glu Ile 
            260                 265                 270 

Cys Arg Asp Met Ala Gln Thr Tyr Pro Met His Arg Leu Leu Gln Gly 
        275                 280                 285 

Asp Val Gly Ser Gly Lys Thr Ile Val Ala Ala Leu Ser Ala Leu Thr 
    290                 295                 300 

Ala Ile Glu Ser Gly Ala Gln Val Ala Val Met Ala Pro Thr Glu Ile 
305                 310                 315                 320 

Leu Ala Glu Gln His Phe Ile Lys Phe Lys Gln Trp Leu Glu Pro Leu 
                325                 330                 335 

Gly Ile Glu Val Val Arg Leu Phe Gly Ser Leu Arg Lys Lys Ala Lys 
            340                 345                 350 

Asp Glu Ala Lys Ala Lys Leu Ala Asp Gly Ser Val Lys Ile Ala Val 
        355                 360                 365 

Gly Thr His Ala Leu Phe Ser Asp Gly Val Ala Phe His Asn Leu Gly 
    370                 375                 380 

Leu Thr Ile Val Asp Glu Gln His Arg Phe Gly Val Ala Gln Arg Leu 
385                 390                 395                 400 

Ala Leu Lys Asn Lys Gly Arg Glu Val His Gln Leu Met Met Ser Ala 
                405                 410                 415 

Thr Pro Ile Pro Arg Thr Leu Ala Met Ser Phe Phe Ala Asp Leu Asp 
            420                 425                 430 

Val Ser Val Ile Asp Glu Leu Pro Pro Gly Arg Thr Pro Ile Lys Thr 
        435                 440                 445 

Arg Leu Val Asn Asn Val Arg Arg Ala Glu Val Glu Gly Phe Val Leu 
    450                 455                 460 

Gly Thr Cys Arg Lys Gly Arg Gln Ala Tyr Trp Val Cys Pro Leu Ile 
465                 470                 475                 480 

Glu Glu Ser Glu Thr Leu Gln Leu Gln Thr Ala Ala Glu Thr Leu Ala 
                485                 490                 495 

Arg Leu Gln Thr Ala Leu Pro Glu Leu Asn Ile Gly Leu Val His Gly 
            500                 505                 510 

Arg Met Lys Ala Ala Glu Lys Ala Glu Val Met Ala Arg Phe Ser Ser 
        515                 520                 525 

Gly Gly Leu Asn Val Leu Val Ala Thr Thr Val Ile Glu Val Gly Val 
    530                 535                 540 

Asp Val Pro Asn Ala Ala Leu Met Val Ile Glu His Ala Glu Arg Met 
545                 550                 555                 560 

Gly Leu Ala Gln Leu His Gln Leu Arg Gly Arg Val Gly Arg Gly Ala 
                565                 570                 575 

Ala Glu Ser Val Cys Val Leu Leu Phe Ala Glu Pro Leu Gly Glu Leu 
            580                 585                 590 

Ala Lys Ala Arg Leu Lys Val Ile Tyr Glu His Thr Asp Gly Phe Glu 
        595                 600                 605 

Ile Ala Arg Gln Asp Leu Asn Ile Arg Gly Pro Gly Glu Phe Leu Gly 
    610                 615                 620 

Ala Arg Gln Ser Gly Val Pro Met Leu Arg Phe Ala Lys Leu Glu Glu 
625                 630                 635                 640 

Asp Leu His Leu Leu Glu Gln Ala Arg Glu Thr Ala Pro Met Leu Ile 
                645                 650                 655 

Glu Gln Asn Pro Glu Ile Val Glu Ala His Leu Ala Arg Trp Leu Ser 
            660                 665                 670 

Gly Arg Glu Gly Tyr Leu Gly Val 
        675                 680 

 
           
             3  
             1257  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1257)  
             
           
            3 

atg cac gtc tcc gaa tta caa acc ctg cac att tcc aaa ctc tta gaa       48 
Met His Val Ser Glu Leu Gln Thr Leu His Ile Ser Lys Leu Leu Glu 
  1               5                  10                  15 

ttg gcg gaa gaa cac ggc atc gaa aac gcc aac cga ttc cgc aaa caa       96 
Leu Ala Glu Glu His Gly Ile Glu Asn Ala Asn Arg Phe Arg Lys Gln 
             20                  25                  30 

gat ctc gta ttt gcc atc gtc cgc cag atg atg aaa aaa ggc gag ggt      144 
Asp Leu Val Phe Ala Ile Val Arg Gln Met Met Lys Lys Gly Glu Gly 
         35                  40                  45 

ttc acc tgc tcc ggc acg ctt gaa atc ctg ccc gac ggc ttc ggc ttc      192 
Phe Thr Cys Ser Gly Thr Leu Glu Ile Leu Pro Asp Gly Phe Gly Phe 
     50                  55                  60 

ctc cgc agc gcg gac acg tcc tat ctt gcc ggc ccc gac gac atc tat      240 
Leu Arg Ser Ala Asp Thr Ser Tyr Leu Ala Gly Pro Asp Asp Ile Tyr 
 65                  70                  75                  80 

gtc tcg ccc acc caa atc cgc cgc ttc aac ctg cat acg ggc gac acc      288 
Val Ser Pro Thr Gln Ile Arg Arg Phe Asn Leu His Thr Gly Asp Thr 
                 85                  90                  95 

atc gaa gga agc gtg cgc gtc cca aaa gac aac gaa cgc tat ttt gcc      336 
Ile Glu Gly Ser Val Arg Val Pro Lys Asp Asn Glu Arg Tyr Phe Ala 
            100                 105                 110 

ctg gtc agg ctt gat acc atc aac ggc gac cac ccg gaa gta tgc cgc      384 
Leu Val Arg Leu Asp Thr Ile Asn Gly Asp His Pro Glu Val Cys Arg 
        115                 120                 125 

cat aaa atc ctg ttt gaa aac ctg acc ccg ctg ttt ccg acc gaa cag      432 
His Lys Ile Leu Phe Glu Asn Leu Thr Pro Leu Phe Pro Thr Glu Gln 
    130                 135                 140 

ttg aag ctg gaa cgc gac tta aag tcc gaa gaa aac ctg acc gga cgt      480 
Leu Lys Leu Glu Arg Asp Leu Lys Ser Glu Glu Asn Leu Thr Gly Arg 
145                 150                 155                 160 

gcc atc gac ctg att tcc cct atc ggc aaa ggt cag cgc gcc ctc ttg      528 
Ala Ile Asp Leu Ile Ser Pro Ile Gly Lys Gly Gln Arg Ala Leu Leu 
                165                 170                 175 

gtt gcc ccg ccc aaa agc ggt aaa acc gtg atg ctg caa aac att gcc      576 
Val Ala Pro Pro Lys Ser Gly Lys Thr Val Met Leu Gln Asn Ile Ala 
            180                 185                 190 

cac gcc gtt acc tca aac tat ccc gaa gtc gaa ctc atc gtc ctc ttg      624 
His Ala Val Thr Ser Asn Tyr Pro Glu Val Glu Leu Ile Val Leu Leu 
        195                 200                 205 

att gac gag cgt ccc gaa gaa gta acc gaa atg agc cgt tcc gtc cgc      672 
Ile Asp Glu Arg Pro Glu Glu Val Thr Glu Met Ser Arg Ser Val Arg 
    210                 215                 220 

ggc gaa gtg gtt tcc tcc acc ttt gac gaa ccg gcg caa cgc cac gtc      720 
Gly Glu Val Val Ser Ser Thr Phe Asp Glu Pro Ala Gln Arg His Val 
225                 230                 235                 240 

caa gtt gcc gaa atg gtg ctt gaa aaa gcc aag cgt atg gta gaa cac      768 
Gln Val Ala Glu Met Val Leu Glu Lys Ala Lys Arg Met Val Glu His 
                245                 250                 255 

aaa aaa gac gtg gtc atc ctg ctg gat tcg att acc cgc ctt gcc cgc      816 
Lys Lys Asp Val Val Ile Leu Leu Asp Ser Ile Thr Arg Leu Ala Arg 
            260                 265                 270 

gcc tac aat acc gtc gtg cct acc tcg ggc aaa atc cta acc ggc ggt      864 
Ala Tyr Asn Thr Val Val Pro Thr Ser Gly Lys Ile Leu Thr Gly Gly 
        275                 280                 285 

gtc gat gcc aac gca ctg cac cgc ccc aaa cgt ttc ttc ggc gcg gcg      912 
Val Asp Ala Asn Ala Leu His Arg Pro Lys Arg Phe Phe Gly Ala Ala 
    290                 295                 300 

cgc aac gtg gaa gaa ggc ggt tcg ctg acc atc atc gcc acc gca ttg      960 
Arg Asn Val Glu Glu Gly Gly Ser Leu Thr Ile Ile Ala Thr Ala Leu 
305                 310                 315                 320 

gtt gaa acc ggc agc cgt atg gac gat gtg att tac gaa gaa ttc aaa     1008 
Val Glu Thr Gly Ser Arg Met Asp Asp Val Ile Tyr Glu Glu Phe Lys 
                325                 330                 335 

ggc acc ggc aat atg gaa ttg cac ctt gac cgc cgt atg gcg gaa aaa     1056 
Gly Thr Gly Asn Met Glu Leu His Leu Asp Arg Arg Met Ala Glu Lys 
            340                 345                 350 

cgc ctc ttc ccc gcc atc aac atc aac aaa tcc ggc acg cgc cgc gaa     1104 
Arg Leu Phe Pro Ala Ile Asn Ile Asn Lys Ser Gly Thr Arg Arg Glu 
        355                 360                 365 

gag ctg ctt gtg ccg aac gac cag ttg caa cgt atg tgg ctc tta cgc     1152 
Glu Leu Leu Val Pro Asn Asp Gln Leu Gln Arg Met Trp Leu Leu Arg 
    370                 375                 380 

aag ttc ctg cac ccg atg gac gaa atc gag gca gcc gaa ttt tta atc     1200 
Lys Phe Leu His Pro Met Asp Glu Ile Glu Ala Ala Glu Phe Leu Ile 
385                 390                 395                 400 

ggg aaa atc aaa gcc tct aaa aac aat gat gat ttc ttt gaa ctg atg     1248 
Gly Lys Ile Lys Ala Ser Lys Asn Asn Asp Asp Phe Phe Glu Leu Met 
                405                 410                 415 

cgc ggc aaa                                                         1257 
Arg Gly Lys 

 
           
             4  
             419  
             PRT  
             Neisseria meningitidis  
           
            4 

Met His Val Ser Glu Leu Gln Thr Leu His Ile Ser Lys Leu Leu Glu 
  1               5                  10                  15 

Leu Ala Glu Glu His Gly Ile Glu Asn Ala Asn Arg Phe Arg Lys Gln 
             20                  25                  30 

Asp Leu Val Phe Ala Ile Val Arg Gln Met Met Lys Lys Gly Glu Gly 
         35                  40                  45 

Phe Thr Cys Ser Gly Thr Leu Glu Ile Leu Pro Asp Gly Phe Gly Phe 
     50                  55                  60 

Leu Arg Ser Ala Asp Thr Ser Tyr Leu Ala Gly Pro Asp Asp Ile Tyr 
 65                  70                  75                  80 

Val Ser Pro Thr Gln Ile Arg Arg Phe Asn Leu His Thr Gly Asp Thr 
                 85                  90                  95 

Ile Glu Gly Ser Val Arg Val Pro Lys Asp Asn Glu Arg Tyr Phe Ala 
            100                 105                 110 

Leu Val Arg Leu Asp Thr Ile Asn Gly Asp His Pro Glu Val Cys Arg 
        115                 120                 125 

His Lys Ile Leu Phe Glu Asn Leu Thr Pro Leu Phe Pro Thr Glu Gln 
    130                 135                 140 

Leu Lys Leu Glu Arg Asp Leu Lys Ser Glu Glu Asn Leu Thr Gly Arg 
145                 150                 155                 160 

Ala Ile Asp Leu Ile Ser Pro Ile Gly Lys Gly Gln Arg Ala Leu Leu 
                165                 170                 175 

Val Ala Pro Pro Lys Ser Gly Lys Thr Val Met Leu Gln Asn Ile Ala 
            180                 185                 190 

His Ala Val Thr Ser Asn Tyr Pro Glu Val Glu Leu Ile Val Leu Leu 
        195                 200                 205 

Ile Asp Glu Arg Pro Glu Glu Val Thr Glu Met Ser Arg Ser Val Arg 
    210                 215                 220 

Gly Glu Val Val Ser Ser Thr Phe Asp Glu Pro Ala Gln Arg His Val 
225                 230                 235                 240 

Gln Val Ala Glu Met Val Leu Glu Lys Ala Lys Arg Met Val Glu His 
                245                 250                 255 

Lys Lys Asp Val Val Ile Leu Leu Asp Ser Ile Thr Arg Leu Ala Arg 
            260                 265                 270 

Ala Tyr Asn Thr Val Val Pro Thr Ser Gly Lys Ile Leu Thr Gly Gly 
        275                 280                 285 

Val Asp Ala Asn Ala Leu His Arg Pro Lys Arg Phe Phe Gly Ala Ala 
    290                 295                 300 

Arg Asn Val Glu Glu Gly Gly Ser Leu Thr Ile Ile Ala Thr Ala Leu 
305                 310                 315                 320 

Val Glu Thr Gly Ser Arg Met Asp Asp Val Ile Tyr Glu Glu Phe Lys 
                325                 330                 335 

Gly Thr Gly Asn Met Glu Leu His Leu Asp Arg Arg Met Ala Glu Lys 
            340                 345                 350 

Arg Leu Phe Pro Ala Ile Asn Ile Asn Lys Ser Gly Thr Arg Arg Glu 
        355                 360                 365 

Glu Leu Leu Val Pro Asn Asp Gln Leu Gln Arg Met Trp Leu Leu Arg 
    370                 375                 380 

Lys Phe Leu His Pro Met Asp Glu Ile Glu Ala Ala Glu Phe Leu Ile 
385                 390                 395                 400 

Gly Lys Ile Lys Ala Ser Lys Asn Asn Asp Asp Phe Phe Glu Leu Met 
                405                 410                 415 

Arg Gly Lys 

 
           
             5  
             599  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(597)  
             
           
            5 

atg aac aat tta atc aaa atc aac ctc ctc ccc tac agg gaa gag atg       48 
Met Asn Asn Leu Ile Lys Ile Asn Leu Leu Pro Tyr Arg Glu Glu Met 
  1               5                  10                  15 

aac aag cgc aaa cag cag cag ttt aaa acg ctg atg tac ggt gcc gtg       96 
Asn Lys Arg Lys Gln Gln Gln Phe Lys Thr Leu Met Tyr Gly Ala Val 
             20                  25                  30 

ctg acg ggc gtt gcc gcc gtt gcg gca acc tac ctg ttt atc gac aat      144 
Leu Thr Gly Val Ala Ala Val Ala Ala Thr Tyr Leu Phe Ile Asp Asn 
         35                  40                  45 

atg atc aat aac cag tcg gaa aga aac acg ctg ctg gaa acc tcc atc      192 
Met Ile Asn Asn Gln Ser Glu Arg Asn Thr Leu Leu Glu Thr Ser Ile 
     50                  55                  60 

gca cac ttg gat acc gag ctg tcg gaa ata caa aag ctc aaa cag gaa      240 
Ala His Leu Asp Thr Glu Leu Ser Glu Ile Gln Lys Leu Lys Gln Glu 
 65                  70                  75                  80 

aaa gat gcc ttc ctg att aag aaa aac aaa atc gag gag ctc cag ctc      288 
Lys Asp Ala Phe Leu Ile Lys Lys Asn Lys Ile Glu Glu Leu Gln Leu 
                 85                  90                  95 

aaa cgc ctc caa gcc gca aaa atc ctc gac agc ctg aat gag gcc gtc      336 
Lys Arg Leu Gln Ala Ala Lys Ile Leu Asp Ser Leu Asn Glu Ala Val 
            100                 105                 110 

ccc gga agc acc tac ctg acc tcg ctg gat gcc gtt acc gcc gac tct      384 
Pro Gly Ser Thr Tyr Leu Thr Ser Leu Asp Ala Val Thr Ala Asp Ser 
        115                 120                 125 

tat cgg ctc agc ggc agg aca tcc agc gac aac cgc gtt gcc gcc atg      432 
Tyr Arg Leu Ser Gly Arg Thr Ser Ser Asp Asn Arg Val Ala Ala Met 
    130                 135                 140 

atg agg gcg atg ccc aat acc ggc ata ttc aag caa ccc gaa ttg tta      480 
Met Arg Ala Met Pro Asn Thr Gly Ile Phe Lys Gln Pro Glu Leu Leu 
145                 150                 155                 160 

agc atc aag aaa aac aat tcg cat caa gaa ttt acc ctt cag gca aca      528 
Ser Ile Lys Lys Asn Asn Ser His Gln Glu Phe Thr Leu Gln Ala Thr 
                165                 170                 175 

tta caa ccc atc gta aag gcg gcc gaa tcc aaa gag aat ccg gct tcg      576 
Leu Gln Pro Ile Val Lys Ala Ala Glu Ser Lys Glu Asn Pro Ala Ser 
            180                 185                 190 

gga aac gca cag gag gca aac tg                                       599 
Gly Asn Ala Gln Glu Ala Asn 
        195 

 
           
             6  
             199  
             PRT  
             Neisseria meningitidis  
           
            6 

Met Asn Asn Leu Ile Lys Ile Asn Leu Leu Pro Tyr Arg Glu Glu Met 
  1               5                  10                  15 

Asn Lys Arg Lys Gln Gln Gln Phe Lys Thr Leu Met Tyr Gly Ala Val 
             20                  25                  30 

Leu Thr Gly Val Ala Ala Val Ala Ala Thr Tyr Leu Phe Ile Asp Asn 
         35                  40                  45 

Met Ile Asn Asn Gln Ser Glu Arg Asn Thr Leu Leu Glu Thr Ser Ile 
     50                  55                  60 

Ala His Leu Asp Thr Glu Leu Ser Glu Ile Gln Lys Leu Lys Gln Glu 
 65                  70                  75                  80 

Lys Asp Ala Phe Leu Ile Lys Lys Asn Lys Ile Glu Glu Leu Gln Leu 
                 85                  90                  95 

Lys Arg Leu Gln Ala Ala Lys Ile Leu Asp Ser Leu Asn Glu Ala Val 
            100                 105                 110 

Pro Gly Ser Thr Tyr Leu Thr Ser Leu Asp Ala Val Thr Ala Asp Ser 
        115                 120                 125 

Tyr Arg Leu Ser Gly Arg Thr Ser Ser Asp Asn Arg Val Ala Ala Met 
    130                 135                 140 

Met Arg Ala Met Pro Asn Thr Gly Ile Phe Lys Gln Pro Glu Leu Leu 
145                 150                 155                 160 

Ser Ile Lys Lys Asn Asn Ser His Gln Glu Phe Thr Leu Gln Ala Thr 
                165                 170                 175 

Leu Gln Pro Ile Val Lys Ala Ala Glu Ser Lys Glu Asn Pro Ala Ser 
            180                 185                 190 

Gly Asn Ala Gln Glu Ala Asn 
        195 

 
           
             7  
             1773  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1773)  
             
           
            7 

atg agt atc gtg ctg cac ggc gtg gcg gcg ggc aaa ggc att gcc gtc       48 
Met Ser Ile Val Leu His Gly Val Ala Ala Gly Lys Gly Ile Ala Val 
  1               5                  10                  15 

ggt tgc gcc cac ctg att gcg cgc ggt acg gag gaa gtg ccg cag tat       96 
Gly Cys Ala His Leu Ile Ala Arg Gly Thr Glu Glu Val Pro Gln Tyr 
             20                  25                  30 

gat gtt gcg gag gcg gac acc gat gcc gaa gcc gaa cgt ttc gat gcc      144 
Asp Val Ala Glu Ala Asp Thr Asp Ala Glu Ala Glu Arg Phe Asp Ala 
         35                  40                  45 

gcc gtc aaa gcc acg cgc aaa gag ttg gaa cag ctc cgc agc gcg att      192 
Ala Val Lys Ala Thr Arg Lys Glu Leu Glu Gln Leu Arg Ser Ala Ile 
     50                  55                  60 

ccc gaa aac gcc ccg acc gag ttg ggc gcg ttc atc tcg ctg cac ctg      240 
Pro Glu Asn Ala Pro Thr Glu Leu Gly Ala Phe Ile Ser Leu His Leu 
 65                  70                  75                  80 

atg ctc ttg acc gat gtt acc ttg tcg cgc gaa ccc gtc gat att tta      288 
Met Leu Leu Thr Asp Val Thr Leu Ser Arg Glu Pro Val Asp Ile Leu 
                 85                  90                  95 

agg gaa caa aaa atc aac gcc gag tgg gca ttg aag cag cag agc gac      336 
Arg Glu Gln Lys Ile Asn Ala Glu Trp Ala Leu Lys Gln Gln Ser Asp 
            100                 105                 110 

aaa ctc gcc gcc caa ttc gac aat atg gac gat gcc tat ttg cgc gaa      384 
Lys Leu Ala Ala Gln Phe Asp Asn Met Asp Asp Ala Tyr Leu Arg Glu 
        115                 120                 125 

cgc aag cag gat atg ctg caa gtc gtc cgc cgc atc cac aac aac ctg      432 
Arg Lys Gln Asp Met Leu Gln Val Val Arg Arg Ile His Asn Asn Leu 
    130                 135                 140 

atc ggg cag ggc aac gag ttg gaa gtt gcc gac aac ctg ttt gac gaa      480 
Ile Gly Gln Gly Asn Glu Leu Glu Val Ala Asp Asn Leu Phe Asp Glu 
145                 150                 155                 160 

acc gtt ctg att gca aac gac ctt tcg ccc gcc gac acg gtt ttg ttt      528 
Thr Val Leu Ile Ala Asn Asp Leu Ser Pro Ala Asp Thr Val Leu Phe 
                165                 170                 175 

aaa gag cag cgc att gcc gcc ttc gtt acc gat gcc ggc ggc ccc acc      576 
Lys Glu Gln Arg Ile Ala Ala Phe Val Thr Asp Ala Gly Gly Pro Thr 
            180                 185                 190 

ggg cat acg gcg att ttg ggc agg agc ttg gac atc ccg tcc gtc gtc      624 
Gly His Thr Ala Ile Leu Gly Arg Ser Leu Asp Ile Pro Ser Val Val 
        195                 200                 205 

ggg ctg cac aac gcg cgc aaa ctg att acc gag ggc gaa acg gtc att      672 
Gly Leu His Asn Ala Arg Lys Leu Ile Thr Glu Gly Glu Thr Val Ile 
    210                 215                 220 

gtg gac ggt atc aac ggc gtg ttg att atc gcg ccg gat gag tcg gtg      720 
Val Asp Gly Ile Asn Gly Val Leu Ile Ile Ala Pro Asp Glu Ser Val 
225                 230                 235                 240 

ttg aac gaa tac cgc cgc cgt gcc cgc gaa tac cgc agc cac aaa cgc      768 
Leu Asn Glu Tyr Arg Arg Arg Ala Arg Glu Tyr Arg Ser His Lys Arg 
                245                 250                 255 

gat ttg aac aag ctc aaa aaa acc gcc gcc gcc acc gct gac ggg gtc      816 
Asp Leu Asn Lys Leu Lys Lys Thr Ala Ala Ala Thr Ala Asp Gly Val 
            260                 265                 270 

tgc atc gag ctt gtg ggc aat ata gaa tcc gcc gaa gac gtg aaa ccg      864 
Cys Ile Glu Leu Val Gly Asn Ile Glu Ser Ala Glu Asp Val Lys Pro 
        275                 280                 285 

ctg cac aac ctc ggc gca gac ggc atc ggg ctg ttc cgc agc gag ttt      912 
Leu His Asn Leu Gly Ala Asp Gly Ile Gly Leu Phe Arg Ser Glu Phe 
    290                 295                 300 

ctt tac ctg aac cgc gat acg atg ccg tct gaa gac gag cag tac gaa      960 
Leu Tyr Leu Asn Arg Asp Thr Met Pro Ser Glu Asp Glu Gln Tyr Glu 
305                 310                 315                 320 

gtg tac agc gcg att gtc aaa aaa atg aaa ggc aaa agc gta acg ata     1008 
Val Tyr Ser Ala Ile Val Lys Lys Met Lys Gly Lys Ser Val Thr Ile 
                325                 330                 335 

cgg aca gtc gat ttg ggt gtg gac aaa aac ccg cgc tgg ttc ggg aaa     1056 
Arg Thr Val Asp Leu Gly Val Asp Lys Asn Pro Arg Trp Phe Gly Lys 
            340                 345                 350 

aac agc acg ccc aac ggc agc ctc aac ccc gcg ctg ggc atg acc ggc     1104 
Asn Ser Thr Pro Asn Gly Ser Leu Asn Pro Ala Leu Gly Met Thr Gly 
        355                 360                 365 

atc cgc ctg tgc ctt gcc gaa ccg gtc atg ttc cgc acc cag atg cgc     1152 
Ile Arg Leu Cys Leu Ala Glu Pro Val Met Phe Arg Thr Gln Met Arg 
    370                 375                 380 

gcc atc ctc cgc gcc gcc gcc cac ggc ccc gtg cgg atg atg tgg ccg     1200 
Ala Ile Leu Arg Ala Ala Ala His Gly Pro Val Arg Met Met Trp Pro 
385                 390                 395                 400 

atg att acc tcc gta tcc gaa gtg cgc cag tgc ctc atc cac ctc gac     1248 
Met Ile Thr Ser Val Ser Glu Val Arg Gln Cys Leu Ile His Leu Asp 
                405                 410                 415 

acc gcg caa cgc cag ctt gcc gaa cgc ggc gat gcc ttc ggt aaa gtc     1296 
Thr Ala Gln Arg Gln Leu Ala Glu Arg Gly Asp Ala Phe Gly Lys Val 
            420                 425                 430 

ggc atc ggc tgt atg att gaa att ccg tct gcc gcg ctg acc gtc ggc     1344 
Gly Ile Gly Cys Met Ile Glu Ile Pro Ser Ala Ala Leu Thr Val Gly 
        435                 440                 445 

agt att ttg aaa ctg gtc gat ttc atc tcc gtc ggt acc aac gac ctg     1392 
Ser Ile Leu Lys Leu Val Asp Phe Ile Ser Val Gly Thr Asn Asp Leu 
    450                 455                 460 

att caa tac atc ttg tcc gtc gat cgc ggc gac gac agc gtc agc cac     1440 
Ile Gln Tyr Ile Leu Ser Val Asp Arg Gly Asp Asp Ser Val Ser His 
465                 470                 475                 480 

ctc tac cag ccc ggc cat ccc gcc gtg ctg aaa atg ctg caa cac gtc     1488 
Leu Tyr Gln Pro Gly His Pro Ala Val Leu Lys Met Leu Gln His Val 
                485                 490                 495 

atc cgt acc gcc aac cgc atg gac aaa gac gta tcc gta tgc ggc gag     1536 
Ile Arg Thr Ala Asn Arg Met Asp Lys Asp Val Ser Val Cys Gly Glu 
            500                 505                 510 

atg gcg ggc gat acc gcg ttt acc cgc gtt tta ttg ggt atg ggg ctg     1584 
Met Ala Gly Asp Thr Ala Phe Thr Arg Val Leu Leu Gly Met Gly Leu 
        515                 520                 525 

cgc cgt ttt tcc atg aac ccc aac aac atc ctg ccc gtc aaa aac atc     1632 
Arg Arg Phe Ser Met Asn Pro Asn Asn Ile Leu Pro Val Lys Asn Ile 
    530                 535                 540 

att ctg cac agc aat gtc gga cag ctc gaa agc gat att gtg aaa gtc     1680 
Ile Leu His Ser Asn Val Gly Gln Leu Glu Ser Asp Ile Val Lys Val 
545                 550                 555                 560 

atc cgc tgc gaa gac gaa gag aag tcg gaa aag ctg atc aaa cag atg     1728 
Ile Arg Cys Glu Asp Glu Glu Lys Ser Glu Lys Leu Ile Lys Gln Met 
                565                 570                 575 

aac agc gtg tct gtc gag gaa gaa gcc gac ttc aag ggg cgg aaa         1773 
Asn Ser Val Ser Val Glu Glu Glu Ala Asp Phe Lys Gly Arg Lys 
            580                 585                 590 

 
           
             8  
             591  
             PRT  
             Neisseria meningitidis  
           
            8 

Met Ser Ile Val Leu His Gly Val Ala Ala Gly Lys Gly Ile Ala Val 
  1               5                  10                  15 

Gly Cys Ala His Leu Ile Ala Arg Gly Thr Glu Glu Val Pro Gln Tyr 
             20                  25                  30 

Asp Val Ala Glu Ala Asp Thr Asp Ala Glu Ala Glu Arg Phe Asp Ala 
         35                  40                  45 

Ala Val Lys Ala Thr Arg Lys Glu Leu Glu Gln Leu Arg Ser Ala Ile 
     50                  55                  60 

Pro Glu Asn Ala Pro Thr Glu Leu Gly Ala Phe Ile Ser Leu His Leu 
 65                  70                  75                  80 

Met Leu Leu Thr Asp Val Thr Leu Ser Arg Glu Pro Val Asp Ile Leu 
                 85                  90                  95 

Arg Glu Gln Lys Ile Asn Ala Glu Trp Ala Leu Lys Gln Gln Ser Asp 
            100                 105                 110 

Lys Leu Ala Ala Gln Phe Asp Asn Met Asp Asp Ala Tyr Leu Arg Glu 
        115                 120                 125 

Arg Lys Gln Asp Met Leu Gln Val Val Arg Arg Ile His Asn Asn Leu 
    130                 135                 140 

Ile Gly Gln Gly Asn Glu Leu Glu Val Ala Asp Asn Leu Phe Asp Glu 
145                 150                 155                 160 

Thr Val Leu Ile Ala Asn Asp Leu Ser Pro Ala Asp Thr Val Leu Phe 
                165                 170                 175 

Lys Glu Gln Arg Ile Ala Ala Phe Val Thr Asp Ala Gly Gly Pro Thr 
            180                 185                 190 

Gly His Thr Ala Ile Leu Gly Arg Ser Leu Asp Ile Pro Ser Val Val 
        195                 200                 205 

Gly Leu His Asn Ala Arg Lys Leu Ile Thr Glu Gly Glu Thr Val Ile 
    210                 215                 220 

Val Asp Gly Ile Asn Gly Val Leu Ile Ile Ala Pro Asp Glu Ser Val 
225                 230                 235                 240 

Leu Asn Glu Tyr Arg Arg Arg Ala Arg Glu Tyr Arg Ser His Lys Arg 
                245                 250                 255 

Asp Leu Asn Lys Leu Lys Lys Thr Ala Ala Ala Thr Ala Asp Gly Val 
            260                 265                 270 

Cys Ile Glu Leu Val Gly Asn Ile Glu Ser Ala Glu Asp Val Lys Pro 
        275                 280                 285 

Leu His Asn Leu Gly Ala Asp Gly Ile Gly Leu Phe Arg Ser Glu Phe 
    290                 295                 300 

Leu Tyr Leu Asn Arg Asp Thr Met Pro Ser Glu Asp Glu Gln Tyr Glu 
305                 310                 315                 320 

Val Tyr Ser Ala Ile Val Lys Lys Met Lys Gly Lys Ser Val Thr Ile 
                325                 330                 335 

Arg Thr Val Asp Leu Gly Val Asp Lys Asn Pro Arg Trp Phe Gly Lys 
            340                 345                 350 

Asn Ser Thr Pro Asn Gly Ser Leu Asn Pro Ala Leu Gly Met Thr Gly 
        355                 360                 365 

Ile Arg Leu Cys Leu Ala Glu Pro Val Met Phe Arg Thr Gln Met Arg 
    370                 375                 380 

Ala Ile Leu Arg Ala Ala Ala His Gly Pro Val Arg Met Met Trp Pro 
385                 390                 395                 400 

Met Ile Thr Ser Val Ser Glu Val Arg Gln Cys Leu Ile His Leu Asp 
                405                 410                 415 

Thr Ala Gln Arg Gln Leu Ala Glu Arg Gly Asp Ala Phe Gly Lys Val 
            420                 425                 430 

Gly Ile Gly Cys Met Ile Glu Ile Pro Ser Ala Ala Leu Thr Val Gly 
        435                 440                 445 

Ser Ile Leu Lys Leu Val Asp Phe Ile Ser Val Gly Thr Asn Asp Leu 
    450                 455                 460 

Ile Gln Tyr Ile Leu Ser Val Asp Arg Gly Asp Asp Ser Val Ser His 
465                 470                 475                 480 

Leu Tyr Gln Pro Gly His Pro Ala Val Leu Lys Met Leu Gln His Val 
                485                 490                 495 

Ile Arg Thr Ala Asn Arg Met Asp Lys Asp Val Ser Val Cys Gly Glu 
            500                 505                 510 

Met Ala Gly Asp Thr Ala Phe Thr Arg Val Leu Leu Gly Met Gly Leu 
        515                 520                 525 

Arg Arg Phe Ser Met Asn Pro Asn Asn Ile Leu Pro Val Lys Asn Ile 
    530                 535                 540 

Ile Leu His Ser Asn Val Gly Gln Leu Glu Ser Asp Ile Val Lys Val 
545                 550                 555                 560 

Ile Arg Cys Glu Asp Glu Glu Lys Ser Glu Lys Leu Ile Lys Gln Met 
                565                 570                 575 

Asn Ser Val Ser Val Glu Glu Glu Ala Asp Phe Lys Gly Arg Lys 
            580                 585                 590 

 
           
             9  
             1572  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1572)  
             
           
            9 

atg ttc aga cgg tat ctt ccg aac aga cag atg aat atg gtt tcc aaa       48 
Met Phe Arg Arg Tyr Leu Pro Asn Arg Gln Met Asn Met Val Ser Lys 
  1               5                  10                  15 

ctg gac aaa tac tgg cag cac ccc gcc ctc tac tgg cct ttg ctc atc       96 
Leu Asp Lys Tyr Trp Gln His Pro Ala Leu Tyr Trp Pro Leu Leu Ile 
             20                  25                  30 

ctt ttt gcc gcc gcc acc ccc ttt acc ttc gca ccc tac tac cac ttt      144 
Leu Phe Ala Ala Ala Thr Pro Phe Thr Phe Ala Pro Tyr Tyr His Phe 
         35                  40                  45 

tgg ctg atg ccc ttg att ttc ggt gcc ttc gtc cgc ctc atc gaa ctg      192 
Trp Leu Met Pro Leu Ile Phe Gly Ala Phe Val Arg Leu Ile Glu Leu 
     50                  55                  60 

cgt ccg cgt ttt gct gtc tct tcc gcc tac ctg ttc ggc ctg acc gca      240 
Arg Pro Arg Phe Ala Val Ser Ser Ala Tyr Leu Phe Gly Leu Thr Ala 
 65                  70                  75                  80 

tac acg aca cag ttc tac tgg ata cac acc gcc ctg cac gac gtt tcc      288 
Tyr Thr Thr Gln Phe Tyr Trp Ile His Thr Ala Leu His Asp Val Ser 
                 85                  90                  95 

ggc ctg ccc gac ctc tat gcc gta ccg ctg acc ttc cta ctc ccc gcc      336 
Gly Leu Pro Asp Leu Tyr Ala Val Pro Leu Thr Phe Leu Leu Pro Ala 
            100                 105                 110 

tac ctt gcc ctt tat ccg gca ctg tgt ttc tgg ctg tgg aaa aaa ttt      384 
Tyr Leu Ala Leu Tyr Pro Ala Leu Cys Phe Trp Leu Trp Lys Lys Phe 
        115                 120                 125 

acc ctg cct cgg ggc ata aaa atc ggt ttg gta ctg ccc atc ctg tgg      432 
Thr Leu Pro Arg Gly Ile Lys Ile Gly Leu Val Leu Pro Ile Leu Trp 
    130                 135                 140 

aca ctg acc gag ttt gcc cgc gaa cgt ttc ctg acc gga ttc ggc tgg      480 
Thr Leu Thr Glu Phe Ala Arg Glu Arg Phe Leu Thr Gly Phe Gly Trp 
145                 150                 155                 160 

ggc gca atc ggc tac tcc caa atc acc ccg gac agc ccg ctc gcc ggc      528 
Gly Ala Ile Gly Tyr Ser Gln Ile Thr Pro Asp Ser Pro Leu Ala Gly 
                165                 170                 175 

ttt gcc cca ttt ggc ggc atc cac atg gtt aca ctg gca acc gcc ttt      576 
Phe Ala Pro Phe Gly Gly Ile His Met Val Thr Leu Ala Thr Ala Phe 
            180                 185                 190 

ctc ggt gtc tgg ctg gtt ttg gcg agt gat aac acc gca cgt tcg ggc      624 
Leu Gly Val Trp Leu Val Leu Ala Ser Asp Asn Thr Ala Arg Ser Gly 
        195                 200                 205 

aaa cgc ctg ctt cca att atc ctg att gcc gcc ctg ctt gcc gca ggc      672 
Lys Arg Leu Leu Pro Ile Ile Leu Ile Ala Ala Leu Leu Ala Ala Gly 
    210                 215                 220 

tac acc gcc cga caa acc gac ttc acc cgc ccc gac ggc agc cgc agc      720 
Tyr Thr Ala Arg Gln Thr Asp Phe Thr Arg Pro Asp Gly Ser Arg Ser 
225                 230                 235                 240 

acc gtc gcc ctg ctt caa ggc aac atc gac caa acc ctc aaa tgg cgt      768 
Thr Val Ala Leu Leu Gln Gly Asn Ile Asp Gln Thr Leu Lys Trp Arg 
                245                 250                 255 

gaa gac caa gtc atc ccg acc ata cag aaa tat tac gaa cag gtc ggc      816 
Glu Asp Gln Val Ile Pro Thr Ile Gln Lys Tyr Tyr Glu Gln Val Gly 
            260                 265                 270 

aaa acc act gcc gac atc gtc atc ctg ccc gaa acc gcc atc ccc gtc      864 
Lys Thr Thr Ala Asp Ile Val Ile Leu Pro Glu Thr Ala Ile Pro Val 
        275                 280                 285 

atg cgc caa aac ctg ccg gaa aac ata ctg gca aaa ttt gcc gaa cag      912 
Met Arg Gln Asn Leu Pro Glu Asn Ile Leu Ala Lys Phe Ala Glu Gln 
    290                 295                 300 

gcg caa aac aac ggc agc gcg ctc gcc gtc ggc atc agc caa tac act      960 
Ala Gln Asn Asn Gly Ser Ala Leu Ala Val Gly Ile Ser Gln Tyr Thr 
305                 310                 315                 320 

tcg gac ggc aac ggt tac gaa aac gcc gtc atc aac ctg acc ggt tat     1008 
Ser Asp Gly Asn Gly Tyr Glu Asn Ala Val Ile Asn Leu Thr Gly Tyr 
                325                 330                 335 

cag gaa aac aat cag gac ggc atc ccc tac tac gcc aaa aac cac ctc     1056 
Gln Glu Asn Asn Gln Asp Gly Ile Pro Tyr Tyr Ala Lys Asn His Leu 
            340                 345                 350 

gtc ccc ttc ggc gaa tac aaa ccg ctg ccc ttc ctg acc acg ccg ctt     1104 
Val Pro Phe Gly Glu Tyr Lys Pro Leu Pro Phe Leu Thr Thr Pro Leu 
        355                 360                 365 

tac aaa atg atg aat atg ccc ctt tcc gac ttc cgc aaa ggc ggc ggt     1152 
Tyr Lys Met Met Asn Met Pro Leu Ser Asp Phe Arg Lys Gly Gly Gly 
    370                 375                 380 

aag caa tcc gcc ctg ctg atg aaa aac caa aaa atc gcc ttc aac atc     1200 
Lys Gln Ser Ala Leu Leu Met Lys Asn Gln Lys Ile Ala Phe Asn Ile 
385                 390                 395                 400 

tgt tac gaa gac gga ttc ggc gac gaa ctg att gcc gcc gcc aaa gat     1248 
Cys Tyr Glu Asp Gly Phe Gly Asp Glu Leu Ile Ala Ala Ala Lys Asp 
                405                 410                 415 

gcc aca ctg ctt gcc aat gcc agc aat atg gcg tgg tac gga aaa tcc     1296 
Ala Thr Leu Leu Ala Asn Ala Ser Asn Met Ala Trp Tyr Gly Lys Ser 
            420                 425                 430 

aac gcc atg tac cag cac ctc caa caa tcg cag gcg cgg gct atg gaa     1344 
Asn Ala Met Tyr Gln His Leu Gln Gln Ser Gln Ala Arg Ala Met Glu 
        435                 440                 445 

ctc gga cgc tat atg gtc cgt gcc acc aac acc ggc gca acc gcc atc     1392 
Leu Gly Arg Tyr Met Val Arg Ala Thr Asn Thr Gly Ala Thr Ala Ile 
    450                 455                 460 

atc tcc ccc aaa ggc aac atc atc gcc caa gcc caa ccc gat acg gaa     1440 
Ile Ser Pro Lys Gly Asn Ile Ile Ala Gln Ala Gln Pro Asp Thr Glu 
465                 470                 475                 480 

acc gta ttg gaa gga cac atc aaa ggc tat gtc ggc gaa acg ccc tat     1488 
Thr Val Leu Glu Gly His Ile Lys Gly Tyr Val Gly Glu Thr Pro Tyr 
                485                 490                 495 

atg aaa acc ggc agt tca tgg tgg ttg atg ggc ata ttg acc cta gcc     1536 
Met Lys Thr Gly Ser Ser Trp Trp Leu Met Gly Ile Leu Thr Leu Ala 
            500                 505                 510 

gca ctg att ctt ttc atc ttc cga aac aaa gaa cac                     1572 
Ala Leu Ile Leu Phe Ile Phe Arg Asn Lys Glu His 
        515                 520 

 
           
             10  
             524  
             PRT  
             Neisseria meningitidis  
           
            10 

Met Phe Arg Arg Tyr Leu Pro Asn Arg Gln Met Asn Met Val Ser Lys 
  1               5                  10                  15 

Leu Asp Lys Tyr Trp Gln His Pro Ala Leu Tyr Trp Pro Leu Leu Ile 
             20                  25                  30 

Leu Phe Ala Ala Ala Thr Pro Phe Thr Phe Ala Pro Tyr Tyr His Phe 
         35                  40                  45 

Trp Leu Met Pro Leu Ile Phe Gly Ala Phe Val Arg Leu Ile Glu Leu 
     50                  55                  60 

Arg Pro Arg Phe Ala Val Ser Ser Ala Tyr Leu Phe Gly Leu Thr Ala 
 65                  70                  75                  80 

Tyr Thr Thr Gln Phe Tyr Trp Ile His Thr Ala Leu His Asp Val Ser 
                 85                  90                  95 

Gly Leu Pro Asp Leu Tyr Ala Val Pro Leu Thr Phe Leu Leu Pro Ala 
            100                 105                 110 

Tyr Leu Ala Leu Tyr Pro Ala Leu Cys Phe Trp Leu Trp Lys Lys Phe 
        115                 120                 125 

Thr Leu Pro Arg Gly Ile Lys Ile Gly Leu Val Leu Pro Ile Leu Trp 
    130                 135                 140 

Thr Leu Thr Glu Phe Ala Arg Glu Arg Phe Leu Thr Gly Phe Gly Trp 
145                 150                 155                 160 

Gly Ala Ile Gly Tyr Ser Gln Ile Thr Pro Asp Ser Pro Leu Ala Gly 
                165                 170                 175 

Phe Ala Pro Phe Gly Gly Ile His Met Val Thr Leu Ala Thr Ala Phe 
            180                 185                 190 

Leu Gly Val Trp Leu Val Leu Ala Ser Asp Asn Thr Ala Arg Ser Gly 
        195                 200                 205 

Lys Arg Leu Leu Pro Ile Ile Leu Ile Ala Ala Leu Leu Ala Ala Gly 
    210                 215                 220 

Tyr Thr Ala Arg Gln Thr Asp Phe Thr Arg Pro Asp Gly Ser Arg Ser 
225                 230                 235                 240 

Thr Val Ala Leu Leu Gln Gly Asn Ile Asp Gln Thr Leu Lys Trp Arg 
                245                 250                 255 

Glu Asp Gln Val Ile Pro Thr Ile Gln Lys Tyr Tyr Glu Gln Val Gly 
            260                 265                 270 

Lys Thr Thr Ala Asp Ile Val Ile Leu Pro Glu Thr Ala Ile Pro Val 
        275                 280                 285 

Met Arg Gln Asn Leu Pro Glu Asn Ile Leu Ala Lys Phe Ala Glu Gln 
    290                 295                 300 

Ala Gln Asn Asn Gly Ser Ala Leu Ala Val Gly Ile Ser Gln Tyr Thr 
305                 310                 315                 320 

Ser Asp Gly Asn Gly Tyr Glu Asn Ala Val Ile Asn Leu Thr Gly Tyr 
                325                 330                 335 

Gln Glu Asn Asn Gln Asp Gly Ile Pro Tyr Tyr Ala Lys Asn His Leu 
            340                 345                 350 

Val Pro Phe Gly Glu Tyr Lys Pro Leu Pro Phe Leu Thr Thr Pro Leu 
        355                 360                 365 

Tyr Lys Met Met Asn Met Pro Leu Ser Asp Phe Arg Lys Gly Gly Gly 
    370                 375                 380 

Lys Gln Ser Ala Leu Leu Met Lys Asn Gln Lys Ile Ala Phe Asn Ile 
385                 390                 395                 400 

Cys Tyr Glu Asp Gly Phe Gly Asp Glu Leu Ile Ala Ala Ala Lys Asp 
                405                 410                 415 

Ala Thr Leu Leu Ala Asn Ala Ser Asn Met Ala Trp Tyr Gly Lys Ser 
            420                 425                 430 

Asn Ala Met Tyr Gln His Leu Gln Gln Ser Gln Ala Arg Ala Met Glu 
        435                 440                 445 

Leu Gly Arg Tyr Met Val Arg Ala Thr Asn Thr Gly Ala Thr Ala Ile 
    450                 455                 460 

Ile Ser Pro Lys Gly Asn Ile Ile Ala Gln Ala Gln Pro Asp Thr Glu 
465                 470                 475                 480 

Thr Val Leu Glu Gly His Ile Lys Gly Tyr Val Gly Glu Thr Pro Tyr 
                485                 490                 495 

Met Lys Thr Gly Ser Ser Trp Trp Leu Met Gly Ile Leu Thr Leu Ala 
            500                 505                 510 

Ala Leu Ile Leu Phe Ile Phe Arg Asn Lys Glu His 
        515                 520 

 
           
             11  
             1185  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(1185)  
             
           
            11 

atg aca gag gac gaa cgt ttc gcg tgg ctg caa ttg gcg ttt acg ccc       48 
Met Thr Glu Asp Glu Arg Phe Ala Trp Leu Gln Leu Ala Phe Thr Pro 
  1               5                  10                  15 

tat atc ggc gcg gaa agt ttc ctg ctg ctg atg cgc cgt ttc ggc agc       96 
Tyr Ile Gly Ala Glu Ser Phe Leu Leu Leu Met Arg Arg Phe Gly Ser 
             20                  25                  30 

gcg caa aat gcc ctg tcc gca ccg gcg gaa cag gtg gcg gca ctg ata      144 
Ala Gln Asn Ala Leu Ser Ala Pro Ala Glu Gln Val Ala Ala Leu Ile 
         35                  40                  45 

cgg cac aaa cag gcg ctt gag gct tgg cgc aat gcg gaa aaa cgc gct      192 
Arg His Lys Gln Ala Leu Glu Ala Trp Arg Asn Ala Glu Lys Arg Ala 
     50                  55                  60 

ctg gcg cgg cag gcg gca gaa gcg gcg ttg gaa tgg gaa atg cgg gac      240 
Leu Ala Arg Gln Ala Ala Glu Ala Ala Leu Glu Trp Glu Met Arg Asp 
 65                  70                  75                  80 

gga tgc cgc ctg atg ctg ctt cag gat gaa gat ttc ccc gaa atg ctg      288 
Gly Cys Arg Leu Met Leu Leu Gln Asp Glu Asp Phe Pro Glu Met Leu 
                 85                  90                  95 

acg cag ggg ctg acc gcg ccg ccg gtt ttg ttt ttg cgc ggc aac gtg      336 
Thr Gln Gly Leu Thr Ala Pro Pro Val Leu Phe Leu Arg Gly Asn Val 
            100                 105                 110 

cgg ctg ctg cac aag cct tcc gcc gcc atc gtc ggc agc cgc cat gcc      384 
Arg Leu Leu His Lys Pro Ser Ala Ala Ile Val Gly Ser Arg His Ala 
        115                 120                 125 

acg ccg cag gcg atg cgg att gcc aaa gat ttc ggc aag tcg ttg ggc      432 
Thr Pro Gln Ala Met Arg Ile Ala Lys Asp Phe Gly Lys Ser Leu Gly 
    130                 135                 140 

ggg caa aat atc ccc gtt gtg tcg ggt atg gct tcc ggt atc gat act      480 
Gly Gln Asn Ile Pro Val Val Ser Gly Met Ala Ser Gly Ile Asp Thr 
145                 150                 155                 160 

gcc gcc cat cag ggc gca ctg gag gca gaa ggc ggc acc atc gcc gtg      528 
Ala Ala His Gln Gly Ala Leu Glu Ala Glu Gly Gly Thr Ile Ala Val 
                165                 170                 175 

tgg ggg acg ggt ata gac cgc att tac ccg ccg tcc aat aaa aac ctt      576 
Trp Gly Thr Gly Ile Asp Arg Ile Tyr Pro Pro Ser Asn Lys Asn Leu 
            180                 185                 190 

gcc tat gaa atc gcc gaa aaa gga ttg att gtc agc gag ttt ccc atc      624 
Ala Tyr Glu Ile Ala Glu Lys Gly Leu Ile Val Ser Glu Phe Pro Ile 
        195                 200                 205 

ggt acg cgc ccg tat gcc ggc aat ttt ccg cgc cgc aac cgc ctg att      672 
Gly Thr Arg Pro Tyr Ala Gly Asn Phe Pro Arg Arg Asn Arg Leu Ile 
    210                 215                 220 

gcc gcc ctg tcg caa gta acg ctg gtg gtt gaa gcc gcc ttg gaa tcc      720 
Ala Ala Leu Ser Gln Val Thr Leu Val Val Glu Ala Ala Leu Glu Ser 
225                 230                 235                 240 

ggt tcg ctg att acc gcc ggc ctg gcg gca gaa atg ggg cgc gaa gtg      768 
Gly Ser Leu Ile Thr Ala Gly Leu Ala Ala Glu Met Gly Arg Glu Val 
                245                 250                 255 

atg gcg gta ccc ggt tcg ata gac aat ccg cac agc aaa ggc tgc cac      816 
Met Ala Val Pro Gly Ser Ile Asp Asn Pro His Ser Lys Gly Cys His 
            260                 265                 270 

aaa ctg att aaa gac ggc gca aaa ttg gtg gaa tgc ctg gac gac atc      864 
Lys Leu Ile Lys Asp Gly Ala Lys Leu Val Glu Cys Leu Asp Asp Ile 
        275                 280                 285 

ctg aac gaa tgc ccg ggg cta ttg caa aat acg ggt gct tca tca tat      912 
Leu Asn Glu Cys Pro Gly Leu Leu Gln Asn Thr Gly Ala Ser Ser Tyr 
    290                 295                 300 

tct ata aat aag gat acg ccc gac acg gga cgc cgc gcc gtt cag acg      960 
Ser Ile Asn Lys Asp Thr Pro Asp Thr Gly Arg Arg Ala Val Gln Thr 
305                 310                 315                 320 

gca tac gcc ccg ccg cct gcc gca aaa atg ccg tct gaa gcg gcg gca     1008 
Ala Tyr Ala Pro Pro Pro Ala Ala Lys Met Pro Ser Glu Ala Ala Ala 
                325                 330                 335 

ggc ggc aca gcc gtc ggc ggc ata ctg gat aaa atg ggt ttc gac ccc     1056 
Gly Gly Thr Ala Val Gly Gly Ile Leu Asp Lys Met Gly Phe Asp Pro 
            340                 345                 350 

atc cat ccc gac gtg ctt gcc gga cag ttg gct atg cct gcc gca gat     1104 
Ile His Pro Asp Val Leu Ala Gly Gln Leu Ala Met Pro Ala Ala Asp 
        355                 360                 365 

ttg tat gcc gca ctg ttg gaa ttg gaa ttg gac ggc agc gtt gcc gca     1152 
Leu Tyr Ala Ala Leu Leu Glu Leu Glu Leu Asp Gly Ser Val Ala Ala 
    370                 375                 380 

atg ccc agc ggc aga tac cag cgt atc cga act                         1185 
Met Pro Ser Gly Arg Tyr Gln Arg Ile Arg Thr 
385                 390                 395 

 
           
             12  
             395  
             PRT  
             Neisseria meningitidis  
           
            12 

Met Thr Glu Asp Glu Arg Phe Ala Trp Leu Gln Leu Ala Phe Thr Pro 
  1               5                  10                  15 

Tyr Ile Gly Ala Glu Ser Phe Leu Leu Leu Met Arg Arg Phe Gly Ser 
             20                  25                  30 

Ala Gln Asn Ala Leu Ser Ala Pro Ala Glu Gln Val Ala Ala Leu Ile 
         35                  40                  45 

Arg His Lys Gln Ala Leu Glu Ala Trp Arg Asn Ala Glu Lys Arg Ala 
     50                  55                  60 

Leu Ala Arg Gln Ala Ala Glu Ala Ala Leu Glu Trp Glu Met Arg Asp 
 65                  70                  75                  80 

Gly Cys Arg Leu Met Leu Leu Gln Asp Glu Asp Phe Pro Glu Met Leu 
                 85                  90                  95 

Thr Gln Gly Leu Thr Ala Pro Pro Val Leu Phe Leu Arg Gly Asn Val 
            100                 105                 110 

Arg Leu Leu His Lys Pro Ser Ala Ala Ile Val Gly Ser Arg His Ala 
        115                 120                 125 

Thr Pro Gln Ala Met Arg Ile Ala Lys Asp Phe Gly Lys Ser Leu Gly 
    130                 135                 140 

Gly Gln Asn Ile Pro Val Val Ser Gly Met Ala Ser Gly Ile Asp Thr 
145                 150                 155                 160 

Ala Ala His Gln Gly Ala Leu Glu Ala Glu Gly Gly Thr Ile Ala Val 
                165                 170                 175 

Trp Gly Thr Gly Ile Asp Arg Ile Tyr Pro Pro Ser Asn Lys Asn Leu 
            180                 185                 190 

Ala Tyr Glu Ile Ala Glu Lys Gly Leu Ile Val Ser Glu Phe Pro Ile 
        195                 200                 205 

Gly Thr Arg Pro Tyr Ala Gly Asn Phe Pro Arg Arg Asn Arg Leu Ile 
    210                 215                 220 

Ala Ala Leu Ser Gln Val Thr Leu Val Val Glu Ala Ala Leu Glu Ser 
225                 230                 235                 240 

Gly Ser Leu Ile Thr Ala Gly Leu Ala Ala Glu Met Gly Arg Glu Val 
                245                 250                 255 

Met Ala Val Pro Gly Ser Ile Asp Asn Pro His Ser Lys Gly Cys His 
            260                 265                 270 

Lys Leu Ile Lys Asp Gly Ala Lys Leu Val Glu Cys Leu Asp Asp Ile 
        275                 280                 285 

Leu Asn Glu Cys Pro Gly Leu Leu Gln Asn Thr Gly Ala Ser Ser Tyr 
    290                 295                 300 

Ser Ile Asn Lys Asp Thr Pro Asp Thr Gly Arg Arg Ala Val Gln Thr 
305                 310                 315                 320 

Ala Tyr Ala Pro Pro Pro Ala Ala Lys Met Pro Ser Glu Ala Ala Ala 
                325                 330                 335 

Gly Gly Thr Ala Val Gly Gly Ile Leu Asp Lys Met Gly Phe Asp Pro 
            340                 345                 350 

Ile His Pro Asp Val Leu Ala Gly Gln Leu Ala Met Pro Ala Ala Asp 
        355                 360                 365 

Leu Tyr Ala Ala Leu Leu Glu Leu Glu Leu Asp Gly Ser Val Ala Ala 
    370                 375                 380 

Met Pro Ser Gly Arg Tyr Gln Arg Ile Arg Thr 
385                 390                 395 

 
           
             13  
             804  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(804)  
             
           
            13 

atg ccg aca gtt ttc atc ttt ttg acg gcg gtt tac gca gga ttg ggt       48 
Met Pro Thr Val Phe Ile Phe Leu Thr Ala Val Tyr Ala Gly Leu Gly 
  1               5                  10                  15 

gca ttt gca tgg cac tgc caa cag cag ggg ctc ggc cgg gat tac ccg       96 
Ala Phe Ala Trp His Cys Gln Gln Gln Gly Leu Gly Arg Asp Tyr Pro 
             20                  25                  30 

tgg aag acg gaa ttg ccg gtt ttg ggc gcg gca ttg acc gtc cat ggc      144 
Trp Lys Thr Glu Leu Pro Val Leu Gly Ala Ala Leu Thr Val His Gly 
         35                  40                  45 

gcg gca ctg ctt atg ccg gtc att caa gac aaa atc atc att atg ggc      192 
Ala Ala Leu Leu Met Pro Val Ile Gln Asp Lys Ile Ile Ile Met Gly 
     50                  55                  60 

ttt ggg tat tcc ggc agc ctg att gtt tgg atg atg ctg ttt att tat      240 
Phe Gly Tyr Ser Gly Ser Leu Ile Val Trp Met Met Leu Phe Ile Tyr 
 65                  70                  75                  80 

ttt gcc ggc agc ttc ttt tat tcg ctg cgc gga gtg cag ttg ctg ctg      288 
Phe Ala Gly Ser Phe Phe Tyr Ser Leu Arg Gly Val Gln Leu Leu Leu 
                 85                  90                  95 

tat cct tgc gcc gca ctg atg ctg ctg tca ggt ttg gtt ttt cct gga      336 
Tyr Pro Cys Ala Ala Leu Met Leu Leu Ser Gly Leu Val Phe Pro Gly 
            100                 105                 110 

aaa ttc tcg gga tat gaa att acc gac ctt ccc ttt atg ctg cat atc      384 
Lys Phe Ser Gly Tyr Glu Ile Thr Asp Leu Pro Phe Met Leu His Ile 
        115                 120                 125 

gga act tcg ctg ctt gca tac ggg ctg ttc ggc atc gca aca tta ttg      432 
Gly Thr Ser Leu Leu Ala Tyr Gly Leu Phe Gly Ile Ala Thr Leu Leu 
    130                 135                 140 

tcc gtt ttg acc ctg ctg ctg aat cgg agc ctg cac cgc agg aac ttc      480 
Ser Val Leu Thr Leu Leu Leu Asn Arg Ser Leu His Arg Arg Asn Phe 
145                 150                 155                 160 

tcc aag ctc gca gga ttc ctg ccg tcg ctg ctc agt ttg gaa aaa ctc      528 
Ser Lys Leu Ala Gly Phe Leu Pro Ser Leu Leu Ser Leu Glu Lys Leu 
                165                 170                 175 

atg ttc cag gcc atg tgg gca ggt ttc atc ctg ctg acc tat tcc gtc      576 
Met Phe Gln Ala Met Trp Ala Gly Phe Ile Leu Leu Thr Tyr Ser Val 
            180                 185                 190 

gtc agc gga aca ttt ttt gcc gaa gcc gta ttc ggc aaa ccc atg acc      624 
Val Ser Gly Thr Phe Phe Ala Glu Ala Val Phe Gly Lys Pro Met Thr 
        195                 200                 205 

ttt acc cat aaa acc gta ttc ggc ata ttg tca tgg ctg att tac ggc      672 
Phe Thr His Lys Thr Val Phe Gly Ile Leu Ser Trp Leu Ile Tyr Gly 
    210                 215                 220 

gga ctg ctg ctc aag cac agc atg acc gca tgg cgc ggc aaa aaa gcc      720 
Gly Leu Leu Leu Lys His Ser Met Thr Ala Trp Arg Gly Lys Lys Ala 
225                 230                 235                 240 

gcc gtg tgg acc atc atc gga ttt gtc agc ctg atg att gcc tat atg      768 
Ala Val Trp Thr Ile Ile Gly Phe Val Ser Leu Met Ile Ala Tyr Met 
                245                 250                 255 

ggc agc aag ttc gta ttg gaa atc att ctg aac aga                      804 
Gly Ser Lys Phe Val Leu Glu Ile Ile Leu Asn Arg 
            260                 265 

 
           
             14  
             268  
             PRT  
             Neisseria meningitidis  
           
            14 

Met Pro Thr Val Phe Ile Phe Leu Thr Ala Val Tyr Ala Gly Leu Gly 
  1               5                  10                  15 

Ala Phe Ala Trp His Cys Gln Gln Gln Gly Leu Gly Arg Asp Tyr Pro 
             20                  25                  30 

Trp Lys Thr Glu Leu Pro Val Leu Gly Ala Ala Leu Thr Val His Gly 
         35                  40                  45 

Ala Ala Leu Leu Met Pro Val Ile Gln Asp Lys Ile Ile Ile Met Gly 
     50                  55                  60 

Phe Gly Tyr Ser Gly Ser Leu Ile Val Trp Met Met Leu Phe Ile Tyr 
 65                  70                  75                  80 

Phe Ala Gly Ser Phe Phe Tyr Ser Leu Arg Gly Val Gln Leu Leu Leu 
                 85                  90                  95 

Tyr Pro Cys Ala Ala Leu Met Leu Leu Ser Gly Leu Val Phe Pro Gly 
            100                 105                 110 

Lys Phe Ser Gly Tyr Glu Ile Thr Asp Leu Pro Phe Met Leu His Ile 
        115                 120                 125 

Gly Thr Ser Leu Leu Ala Tyr Gly Leu Phe Gly Ile Ala Thr Leu Leu 
    130                 135                 140 

Ser Val Leu Thr Leu Leu Leu Asn Arg Ser Leu His Arg Arg Asn Phe 
145                 150                 155                 160 

Ser Lys Leu Ala Gly Phe Leu Pro Ser Leu Leu Ser Leu Glu Lys Leu 
                165                 170                 175 

Met Phe Gln Ala Met Trp Ala Gly Phe Ile Leu Leu Thr Tyr Ser Val 
            180                 185                 190 

Val Ser Gly Thr Phe Phe Ala Glu Ala Val Phe Gly Lys Pro Met Thr 
        195                 200                 205 

Phe Thr His Lys Thr Val Phe Gly Ile Leu Ser Trp Leu Ile Tyr Gly 
    210                 215                 220 

Gly Leu Leu Leu Lys His Ser Met Thr Ala Trp Arg Gly Lys Lys Ala 
225                 230                 235                 240 

Ala Val Trp Thr Ile Ile Gly Phe Val Ser Leu Met Ile Ala Tyr Met 
                245                 250                 255 

Gly Ser Lys Phe Val Leu Glu Ile Ile Leu Asn Arg 
            260                 265 

 
           
             15  
             2391  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(2391)  
             
           
            15 

atg tca aca gtc caa atg aat aaa aat att aaa tct tta aat tta cgg       48 
Met Ser Thr Val Gln Met Asn Lys Asn Ile Lys Ser Leu Asn Leu Arg 
  1               5                  10                  15 

gaa aaa gac ccg ttt tta agt cgt gaa aaa cag cgt tat gaa cat cct       96 
Glu Lys Asp Pro Phe Leu Ser Arg Glu Lys Gln Arg Tyr Glu His Pro 
             20                  25                  30 

ttg ccc agt cgg gaa tgg ata atc gag ctg ctt gaa cgt aaa ggc gta      144 
Leu Pro Ser Arg Glu Trp Ile Ile Glu Leu Leu Glu Arg Lys Gly Val 
         35                  40                  45 

cca tcc aag att gaa gct ttg gta cgc gaa ttg tcg att aag gaa gaa      192 
Pro Ser Lys Ile Glu Ala Leu Val Arg Glu Leu Ser Ile Lys Glu Glu 
     50                  55                  60 

gag tac gaa ttt ttc gaa cgt cgt ctg aag gcg atg gcg cgg gac ggt      240 
Glu Tyr Glu Phe Phe Glu Arg Arg Leu Lys Ala Met Ala Arg Asp Gly 
 65                  70                  75                  80 

cag gtt tta atc aac cgt cgg ggc gcg gtt tgc gcg gcg gac aaa ttg      288 
Gln Val Leu Ile Asn Arg Arg Gly Ala Val Cys Ala Ala Asp Lys Leu 
                 85                  90                  95 

gat ttg gtc aaa tgc cgt gtc gag gcg cac aaa gac ggc ttc ggt ttc      336 
Asp Leu Val Lys Cys Arg Val Glu Ala His Lys Asp Gly Phe Gly Phe 
            100                 105                 110 

gcc gtg ccg ctc acg ccc gcc aaa gac ggt gat ttt gtt ttg tac gaa      384 
Ala Val Pro Leu Thr Pro Ala Lys Asp Gly Asp Phe Val Leu Tyr Glu 
        115                 120                 125 

cgc cag atg cgc ggc att atg cac ggc gat att gtc act gtt cgt cct      432 
Arg Gln Met Arg Gly Ile Met His Gly Asp Ile Val Thr Val Arg Pro 
    130                 135                 140 

gcc ggc atg gac cgt agg ggc cgc cgc gaa ggg acg gtt ctg gat att      480 
Ala Gly Met Asp Arg Arg Gly Arg Arg Glu Gly Thr Val Leu Asp Ile 
145                 150                 155                 160 

gtc gaa cgc gcg caa agc aaa gtg gtc ggc cgt ttc tat atg gat agg      528 
Val Glu Arg Ala Gln Ser Lys Val Val Gly Arg Phe Tyr Met Asp Arg 
                165                 170                 175 

ggc gtg gcg att ttg gag ccg gaa gac aag cgt ctg aac caa agc atc      576 
Gly Val Ala Ile Leu Glu Pro Glu Asp Lys Arg Leu Asn Gln Ser Ile 
            180                 185                 190 

gta ttg gaa ccg gac ggc gtg gcg cgt ttc aaa cct gaa tcc ggt cag      624 
Val Leu Glu Pro Asp Gly Val Ala Arg Phe Lys Pro Glu Ser Gly Gln 
        195                 200                 205 

gtc atc gtc ggc gaa att gag gtt tat cct gag caa aac cgg ccg gca      672 
Val Ile Val Gly Glu Ile Glu Val Tyr Pro Glu Gln Asn Arg Pro Ala 
    210                 215                 220 

gtg gca aaa atc atc gaa gtt ttg ggc gat tat gcc gac agc ggc atg      720 
Val Ala Lys Ile Ile Glu Val Leu Gly Asp Tyr Ala Asp Ser Gly Met 
225                 230                 235                 240 

gag att gaa att gcc gtg cgc aag cat cat ttg ccg cac caa ttc agt      768 
Glu Ile Glu Ile Ala Val Arg Lys His His Leu Pro His Gln Phe Ser 
                245                 250                 255 

gaa gcg tgt gcc aaa gcc gcg aaa aaa att ccc gac cat gta cgc aaa      816 
Glu Ala Cys Ala Lys Ala Ala Lys Lys Ile Pro Asp His Val Arg Lys 
            260                 265                 270 

agc gat ttg aaa ggc cgc gtc gat ttg cgc gac ctg cct ttg gta acg      864 
Ser Asp Leu Lys Gly Arg Val Asp Leu Arg Asp Leu Pro Leu Val Thr 
        275                 280                 285 

ata gac ggc gaa acg gct cga gat ttt gac gat gcg gtg ttt gcc gag      912 
Ile Asp Gly Glu Thr Ala Arg Asp Phe Asp Asp Ala Val Phe Ala Glu 
    290                 295                 300 

aaa atc gga cgc aat tac cgt ctg gtc gtg gcg att gcc gat gtc agc      960 
Lys Ile Gly Arg Asn Tyr Arg Leu Val Val Ala Ile Ala Asp Val Ser 
305                 310                 315                 320 

cat tat gtc cgc ccc gat gac gct atc gac acg gac gct cag gaa cgc     1008 
His Tyr Val Arg Pro Asp Asp Ala Ile Asp Thr Asp Ala Gln Glu Arg 
                325                 330                 335 

agc acc agt gtt tac ttc ccg cgc cgc gtg att ccc atg ttg ccg gaa     1056 
Ser Thr Ser Val Tyr Phe Pro Arg Arg Val Ile Pro Met Leu Pro Glu 
            340                 345                 350 

aac ctg tcc aac ggc atc tgc tcg ctc aat cct cat gtc gag cgt ttg     1104 
Asn Leu Ser Asn Gly Ile Cys Ser Leu Asn Pro His Val Glu Arg Leu 
        355                 360                 365 

tgt gtg gtg tgc gat atg gtt atc act tac gcg ggc aat atc aaa gaa     1152 
Cys Val Val Cys Asp Met Val Ile Thr Tyr Ala Gly Asn Ile Lys Glu 
    370                 375                 380 

tac cgc ttc tac ccc gcc gtg atg cgc tct cat gcc cgc ctg acc tac     1200 
Tyr Arg Phe Tyr Pro Ala Val Met Arg Ser His Ala Arg Leu Thr Tyr 
385                 390                 395                 400 

aac caa gtt tgg aaa tgg ctt tca ggc ggc atc gag cat ccg ttc aaa     1248 
Asn Gln Val Trp Lys Trp Leu Ser Gly Gly Ile Glu His Pro Phe Lys 
                405                 410                 415 

acc caa atc gac acg ctt tac aaa ctc ttc aaa atc ctt cag aaa aag     1296 
Thr Gln Ile Asp Thr Leu Tyr Lys Leu Phe Lys Ile Leu Gln Lys Lys 
            420                 425                 430 

cgt ttc gaa cgc ggg gcg gtg gag ttt gac agc atc gaa acc caa atg     1344 
Arg Phe Glu Arg Gly Ala Val Glu Phe Asp Ser Ile Glu Thr Gln Met 
        435                 440                 445 

ctt ttc gac gac aac ggt aaa att gaa aaa atc gtc ccc gtt gtc cgc     1392 
Leu Phe Asp Asp Asn Gly Lys Ile Glu Lys Ile Val Pro Val Val Arg 
    450                 455                 460 

aac gat gcc cac aag ctg att gaa gaa tgt atg ttg gcg gca aac gtt     1440 
Asn Asp Ala His Lys Leu Ile Glu Glu Cys Met Leu Ala Ala Asn Val 
465                 470                 475                 480 

tgc gca gcg gat ttt ctg ttg aaa aac aag cat acc gca ttg ttc cgc     1488 
Cys Ala Ala Asp Phe Leu Leu Lys Asn Lys His Thr Ala Leu Phe Arg 
                485                 490                 495 

aac cat ttg ggg ccc acg ccc gaa aaa ctc gcc gcc ttg cgc gag cag     1536 
Asn His Leu Gly Pro Thr Pro Glu Lys Leu Ala Ala Leu Arg Glu Gln 
            500                 505                 510 

ctc ggt ctg ttg ggg ctt caa ctt ggc ggc ggc gac aac ccg tcg ccg     1584 
Leu Gly Leu Leu Gly Leu Gln Leu Gly Gly Gly Asp Asn Pro Ser Pro 
        515                 520                 525 

aaa gac tat gcc gcg ctt gcc gga cag ttc aaa ggc agg ccg gat gcc     1632 
Lys Asp Tyr Ala Ala Leu Ala Gly Gln Phe Lys Gly Arg Pro Asp Ala 
    530                 535                 540 

gaa ttg ctg caa gtc atg atg ttg cgc tcc atg caa cag gcg gtt tac     1680 
Glu Leu Leu Gln Val Met Met Leu Arg Ser Met Gln Gln Ala Val Tyr 
545                 550                 555                 560 

gaa ccg cat tgc gac gga cac ttt ggt ctt gcc tac gaa gca tac gcc     1728 
Glu Pro His Cys Asp Gly His Phe Gly Leu Ala Tyr Glu Ala Tyr Ala 
                565                 570                 575 

cac ttc acc tcg ccc atc cgc cgc tat ccc gac ctg acc gta cac cgc     1776 
His Phe Thr Ser Pro Ile Arg Arg Tyr Pro Asp Leu Thr Val His Arg 
            580                 585                 590 

gcc atc aaa gcc gtg ttg aat cag caa acc tac acg cca aaa aaa agc     1824 
Ala Ile Lys Ala Val Leu Asn Gln Gln Thr Tyr Thr Pro Lys Lys Ser 
        595                 600                 605 

tgg cag gct ttg ggc gtg cat acc tcg ttc tgt gag cgc cgt gcc gac     1872 
Trp Gln Ala Leu Gly Val His Thr Ser Phe Cys Glu Arg Arg Ala Asp 
    610                 615                 620 

gac gcc agc cgc gac gtg gaa aac tgg ctg aaa acc tat tat atg cgc     1920 
Asp Ala Ser Arg Asp Val Glu Asn Trp Leu Lys Thr Tyr Tyr Met Arg 
625                 630                 635                 640 

gat aag gtc ggc gaa gta ttc gaa ggt aaa atc tcc ggc atg acc agt     1968 
Asp Lys Val Gly Glu Val Phe Glu Gly Lys Ile Ser Gly Met Thr Ser 
                645                 650                 655 

ttt ggt atc ttt gta aca ctg gac ggc atc cac att gac ggc ttg gtg     2016 
Phe Gly Ile Phe Val Thr Leu Asp Gly Ile His Ile Asp Gly Leu Val 
            660                 665                 670 

cat atc agc gat ttg ggc gaa gac tat ttc aac ttc cgc ccc gaa atc     2064 
His Ile Ser Asp Leu Gly Glu Asp Tyr Phe Asn Phe Arg Pro Glu Ile 
        675                 680                 685 

atg gca atc gaa ggc gaa cgc agc ggc atc cgt ttc aac atg ggg gac     2112 
Met Ala Ile Glu Gly Glu Arg Ser Gly Ile Arg Phe Asn Met Gly Asp 
    690                 695                 700 

agg gtt gcc gtc cgg gtc gcc cgt gcc gat ttg gat gac gga aaa atc     2160 
Arg Val Ala Val Arg Val Ala Arg Ala Asp Leu Asp Asp Gly Lys Ile 
705                 710                 715                 720 

gat ttt gtc ctg att gcc ggg ggg agc ggc agg ggg cgg aaa gtt aaa     2208 
Asp Phe Val Leu Ile Ala Gly Gly Ser Gly Arg Gly Arg Lys Val Lys 
                725                 730                 735 

tca tcc gcg tct gcc aaa ccg gca ggg acg gcg ggg aaa ggg aag ccg     2256 
Ser Ser Ala Ser Ala Lys Pro Ala Gly Thr Ala Gly Lys Gly Lys Pro 
            740                 745                 750 

aaa acc gcc gcc gag aaa aaa aca gcc cga ggc ggc aaa gta agg gga     2304 
Lys Thr Ala Ala Glu Lys Lys Thr Ala Arg Gly Gly Lys Val Arg Gly 
        755                 760                 765 

agg ggc gcg tct gcc gcc gca gaa tcg agg aaa aag gca aag aaa ccg     2352 
Arg Gly Ala Ser Ala Ala Ala Glu Ser Arg Lys Lys Ala Lys Lys Pro 
    770                 775                 780 

gtt ccg att aag gta aaa aaa cgg aaa ggc aaa tca taa                 2391 
Val Pro Ile Lys Val Lys Lys Arg Lys Gly Lys Ser 
785                 790                 795 

 
           
             16  
             796  
             PRT  
             Neisseria meningitidis  
           
            16 

Met Ser Thr Val Gln Met Asn Lys Asn Ile Lys Ser Leu Asn Leu Arg 
  1               5                  10                  15 

Glu Lys Asp Pro Phe Leu Ser Arg Glu Lys Gln Arg Tyr Glu His Pro 
             20                  25                  30 

Leu Pro Ser Arg Glu Trp Ile Ile Glu Leu Leu Glu Arg Lys Gly Val 
         35                  40                  45 

Pro Ser Lys Ile Glu Ala Leu Val Arg Glu Leu Ser Ile Lys Glu Glu 
     50                  55                  60 

Glu Tyr Glu Phe Phe Glu Arg Arg Leu Lys Ala Met Ala Arg Asp Gly 
 65                  70                  75                  80 

Gln Val Leu Ile Asn Arg Arg Gly Ala Val Cys Ala Ala Asp Lys Leu 
                 85                  90                  95 

Asp Leu Val Lys Cys Arg Val Glu Ala His Lys Asp Gly Phe Gly Phe 
            100                 105                 110 

Ala Val Pro Leu Thr Pro Ala Lys Asp Gly Asp Phe Val Leu Tyr Glu 
        115                 120                 125 

Arg Gln Met Arg Gly Ile Met His Gly Asp Ile Val Thr Val Arg Pro 
    130                 135                 140 

Ala Gly Met Asp Arg Arg Gly Arg Arg Glu Gly Thr Val Leu Asp Ile 
145                 150                 155                 160 

Val Glu Arg Ala Gln Ser Lys Val Val Gly Arg Phe Tyr Met Asp Arg 
                165                 170                 175 

Gly Val Ala Ile Leu Glu Pro Glu Asp Lys Arg Leu Asn Gln Ser Ile 
            180                 185                 190 

Val Leu Glu Pro Asp Gly Val Ala Arg Phe Lys Pro Glu Ser Gly Gln 
        195                 200                 205 

Val Ile Val Gly Glu Ile Glu Val Tyr Pro Glu Gln Asn Arg Pro Ala 
    210                 215                 220 

Val Ala Lys Ile Ile Glu Val Leu Gly Asp Tyr Ala Asp Ser Gly Met 
225                 230                 235                 240 

Glu Ile Glu Ile Ala Val Arg Lys His His Leu Pro His Gln Phe Ser 
                245                 250                 255 

Glu Ala Cys Ala Lys Ala Ala Lys Lys Ile Pro Asp His Val Arg Lys 
            260                 265                 270 

Ser Asp Leu Lys Gly Arg Val Asp Leu Arg Asp Leu Pro Leu Val Thr 
        275                 280                 285 

Ile Asp Gly Glu Thr Ala Arg Asp Phe Asp Asp Ala Val Phe Ala Glu 
    290                 295                 300 

Lys Ile Gly Arg Asn Tyr Arg Leu Val Val Ala Ile Ala Asp Val Ser 
305                 310                 315                 320 

His Tyr Val Arg Pro Asp Asp Ala Ile Asp Thr Asp Ala Gln Glu Arg 
                325                 330                 335 

Ser Thr Ser Val Tyr Phe Pro Arg Arg Val Ile Pro Met Leu Pro Glu 
            340                 345                 350 

Asn Leu Ser Asn Gly Ile Cys Ser Leu Asn Pro His Val Glu Arg Leu 
        355                 360                 365 

Cys Val Val Cys Asp Met Val Ile Thr Tyr Ala Gly Asn Ile Lys Glu 
    370                 375                 380 

Tyr Arg Phe Tyr Pro Ala Val Met Arg Ser His Ala Arg Leu Thr Tyr 
385                 390                 395                 400 

Asn Gln Val Trp Lys Trp Leu Ser Gly Gly Ile Glu His Pro Phe Lys 
                405                 410                 415 

Thr Gln Ile Asp Thr Leu Tyr Lys Leu Phe Lys Ile Leu Gln Lys Lys 
            420                 425                 430 

Arg Phe Glu Arg Gly Ala Val Glu Phe Asp Ser Ile Glu Thr Gln Met 
        435                 440                 445 

Leu Phe Asp Asp Asn Gly Lys Ile Glu Lys Ile Val Pro Val Val Arg 
    450                 455                 460 

Asn Asp Ala His Lys Leu Ile Glu Glu Cys Met Leu Ala Ala Asn Val 
465                 470                 475                 480 

Cys Ala Ala Asp Phe Leu Leu Lys Asn Lys His Thr Ala Leu Phe Arg 
                485                 490                 495 

Asn His Leu Gly Pro Thr Pro Glu Lys Leu Ala Ala Leu Arg Glu Gln 
            500                 505                 510 

Leu Gly Leu Leu Gly Leu Gln Leu Gly Gly Gly Asp Asn Pro Ser Pro 
        515                 520                 525 

Lys Asp Tyr Ala Ala Leu Ala Gly Gln Phe Lys Gly Arg Pro Asp Ala 
    530                 535                 540 

Glu Leu Leu Gln Val Met Met Leu Arg Ser Met Gln Gln Ala Val Tyr 
545                 550                 555                 560 

Glu Pro His Cys Asp Gly His Phe Gly Leu Ala Tyr Glu Ala Tyr Ala 
                565                 570                 575 

His Phe Thr Ser Pro Ile Arg Arg Tyr Pro Asp Leu Thr Val His Arg 
            580                 585                 590 

Ala Ile Lys Ala Val Leu Asn Gln Gln Thr Tyr Thr Pro Lys Lys Ser 
        595                 600                 605 

Trp Gln Ala Leu Gly Val His Thr Ser Phe Cys Glu Arg Arg Ala Asp 
    610                 615                 620 

Asp Ala Ser Arg Asp Val Glu Asn Trp Leu Lys Thr Tyr Tyr Met Arg 
625                 630                 635                 640 

Asp Lys Val Gly Glu Val Phe Glu Gly Lys Ile Ser Gly Met Thr Ser 
                645                 650                 655 

Phe Gly Ile Phe Val Thr Leu Asp Gly Ile His Ile Asp Gly Leu Val 
            660                 665                 670 

His Ile Ser Asp Leu Gly Glu Asp Tyr Phe Asn Phe Arg Pro Glu Ile 
        675                 680                 685 

Met Ala Ile Glu Gly Glu Arg Ser Gly Ile Arg Phe Asn Met Gly Asp 
    690                 695                 700 

Arg Val Ala Val Arg Val Ala Arg Ala Asp Leu Asp Asp Gly Lys Ile 
705                 710                 715                 720 

Asp Phe Val Leu Ile Ala Gly Gly Ser Gly Arg Gly Arg Lys Val Lys 
                725                 730                 735 

Ser Ser Ala Ser Ala Lys Pro Ala Gly Thr Ala Gly Lys Gly Lys Pro 
            740                 745                 750 

Lys Thr Ala Ala Glu Lys Lys Thr Ala Arg Gly Gly Lys Val Arg Gly 
        755                 760                 765 

Arg Gly Ala Ser Ala Ala Ala Glu Ser Arg Lys Lys Ala Lys Lys Pro 
    770                 775                 780 

Val Pro Ile Lys Val Lys Lys Arg Lys Gly Lys Ser 
785                 790                 795 

 
           
             17  
             252  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(252)  
             
           
            17 

atg gag cag tcg ggc aaa ttc agt tgg tct gcg gca gct ttt tgg gac       48 
Met Glu Gln Ser Gly Lys Phe Ser Trp Ser Ala Ala Ala Phe Trp Asp 
  1               5                  10                  15 

att ccc tac ccc gcc acc agg cgg att gcc tca agt ttg tat tcg acc       96 
Ile Pro Tyr Pro Ala Thr Arg Arg Ile Ala Ser Ser Leu Tyr Ser Thr 
             20                  25                  30 
gaa tat ttt gtc gta tgc ttt cta cgt ttg atg cca ctc tct ccg tgt      144 
Glu Tyr Phe Val Val Cys Phe Leu Arg Leu Met Pro Leu Ser Pro Cys 
         35                  40                  45 

aat ctg tat ttt gtc acc cat ctg cgt acc aat gaa tcg gaa ata gaa      192 
Asn Leu Tyr Phe Val Thr His Leu Arg Thr Asn Glu Ser Glu Ile Glu 
     50                  55                  60 

aga tgg tct gct gtt ccc tgc caa ata gta ttg aac gac ggc aag tcg      240 
Arg Trp Ser Ala Val Pro Cys Gln Ile Val Leu Asn Asp Gly Lys Ser 
 65                  70                  75                  80 

gaa ttc atc tga                                                      252 
Glu Phe Ile 

 
           
             18  
             83  
             PRT  
             Neisseria meningitidis  
           
            18 

Met Glu Gln Ser Gly Lys Phe Ser Trp Ser Ala Ala Ala Phe Trp Asp 
  1               5                  10                  15 

Ile Pro Tyr Pro Ala Thr Arg Arg Ile Ala Ser Ser Leu Tyr Ser Thr 
             20                  25                  30 

Glu Tyr Phe Val Val Cys Phe Leu Arg Leu Met Pro Leu Ser Pro Cys 
         35                  40                  45 

Asn Leu Tyr Phe Val Thr His Leu Arg Thr Asn Glu Ser Glu Ile Glu 
     50                  55                  60 

Arg Trp Ser Ala Val Pro Cys Gln Ile Val Leu Asn Asp Gly Lys Ser 
 65                  70                  75                  80 

Glu Phe Ile 

 
           
             19  
             789  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(789)  
             
           
            19 

atg aat gtt tat ttt ctc ggc ggc ggc aat atg gcg gct gcc gtt gcg       48 
Met Asn Val Tyr Phe Leu Gly Gly Gly Asn Met Ala Ala Ala Val Ala 
  1               5                  10                  15 

ggc gga ttg gtc aaa caa ggc ggt tac cgc atc cat ata gcc aat cgg       96 
Gly Gly Leu Val Lys Gln Gly Gly Tyr Arg Ile His Ile Ala Asn Arg 
             20                  25                  30 

ggt gcg gaa aaa cgc gaa cgt ttg gga aaa gag ttg ggg gtc gaa act      144 
Gly Ala Glu Lys Arg Glu Arg Leu Gly Lys Glu Leu Gly Val Glu Thr 
         35                  40                  45 

tcg gca acc ctg ccg gag ctt cat tcc gac gat gtt tta atc ctt gcc      192 
Ser Ala Thr Leu Pro Glu Leu His Ser Asp Asp Val Leu Ile Leu Ala 
     50                  55                  60 

gtc aaa ccg cag gat atg gaa gcc gcg tgc aaa aat atc cgc acc aat      240 
Val Lys Pro Gln Asp Met Glu Ala Ala Cys Lys Asn Ile Arg Thr Asn 
 65                  70                  75                  80 

ggt gca ttg gtg ctt tct gtc gca gct gga ttg tcg gtt gat acg ctc      288 
Gly Ala Leu Val Leu Ser Val Ala Ala Gly Leu Ser Val Asp Thr Leu 
                 85                  90                  95 

agc cgt tac ctc ggg gga aca cgc cgc att gtc cgg gtt atg ccg aat      336 
Ser Arg Tyr Leu Gly Gly Thr Arg Arg Ile Val Arg Val Met Pro Asn 
            100                 105                 110 

aca ccc gga aaa atc ggg ctg ggc gta tcc ggt atg ttt gcc gaa gcg      384 
Thr Pro Gly Lys Ile Gly Leu Gly Val Ser Gly Met Phe Ala Glu Ala 
        115                 120                 125 

gaa gta tcg gaa aca gac cgc agg att gcc gat cga atc atg aaa tca      432 
Glu Val Ser Glu Thr Asp Arg Arg Ile Ala Asp Arg Ile Met Lys Ser 
    130                 135                 140 

gtc ggt ttg acc gtt tgg ttg gaa gat gaa gcg caa atg cac agc att      480 
Val Gly Leu Thr Val Trp Leu Glu Asp Glu Ala Gln Met His Ser Ile 
145                 150                 155                 160 

acc ggt atc agc ggc agc ggg ccg gca tat gtg ttt tat ctg ctg gac      528 
Thr Gly Ile Ser Gly Ser Gly Pro Ala Tyr Val Phe Tyr Leu Leu Asp 
                165                 170                 175 

gca ttg caa aat gcc gcc atc cga caa ggg ttt gat atg gca gaa gca      576 
Ala Leu Gln Asn Ala Ala Ile Arg Gln Gly Phe Asp Met Ala Glu Ala 
            180                 185                 190 

cgc gcg ctc agt ctg gca acg ttt aaa gga gcg gtt gcc ctt gcc gag      624 
Arg Ala Leu Ser Leu Ala Thr Phe Lys Gly Ala Val Ala Leu Ala Glu 
        195                 200                 205 

cag acg ggt gaa gat ttc gag aag ctt caa aaa aat gta acg tca aaa      672 
Gln Thr Gly Glu Asp Phe Glu Lys Leu Gln Lys Asn Val Thr Ser Lys 
    210                 215                 220 

ggc ggg aca acc cac gaa gcc gtg gaa gct ttc agg cgg cat cgt gtc      720 
Gly Gly Thr Thr His Glu Ala Val Glu Ala Phe Arg Arg His Arg Val 
225                 230                 235                 240 

gcc gaa gcc ata agc gag ggc gtt tgt gcc tgt gtg cgc cgt tcg cag      768 
Ala Glu Ala Ile Ser Glu Gly Val Cys Ala Cys Val Arg Arg Ser Gln 
                245                 250                 255 

gaa atg gaa cgg caa tat caa                                          789 
Glu Met Glu Arg Gln Tyr Gln 
            260 

 
           
             20  
             263  
             PRT  
             Neisseria meningitidis  
           
            20 

Met Asn Val Tyr Phe Leu Gly Gly Gly Asn Met Ala Ala Ala Val Ala 
  1               5                  10                  15 

Gly Gly Leu Val Lys Gln Gly Gly Tyr Arg Ile His Ile Ala Asn Arg 
             20                  25                  30 

Gly Ala Glu Lys Arg Glu Arg Leu Gly Lys Glu Leu Gly Val Glu Thr 
         35                  40                  45 

Ser Ala Thr Leu Pro Glu Leu His Ser Asp Asp Val Leu Ile Leu Ala 
     50                  55                  60 

Val Lys Pro Gln Asp Met Glu Ala Ala Cys Lys Asn Ile Arg Thr Asn 
 65                  70                  75                  80 

Gly Ala Leu Val Leu Ser Val Ala Ala Gly Leu Ser Val Asp Thr Leu 
                 85                  90                  95 

Ser Arg Tyr Leu Gly Gly Thr Arg Arg Ile Val Arg Val Met Pro Asn 
            100                 105                 110 

Thr Pro Gly Lys Ile Gly Leu Gly Val Ser Gly Met Phe Ala Glu Ala 
        115                 120                 125 

Glu Val Ser Glu Thr Asp Arg Arg Ile Ala Asp Arg Ile Met Lys Ser 
    130                 135                 140 

Val Gly Leu Thr Val Trp Leu Glu Asp Glu Ala Gln Met His Ser Ile 
145                 150                 155                 160 

Thr Gly Ile Ser Gly Ser Gly Pro Ala Tyr Val Phe Tyr Leu Leu Asp 
                165                 170                 175 

Ala Leu Gln Asn Ala Ala Ile Arg Gln Gly Phe Asp Met Ala Glu Ala 
            180                 185                 190 

Arg Ala Leu Ser Leu Ala Thr Phe Lys Gly Ala Val Ala Leu Ala Glu 
        195                 200                 205 

Gln Thr Gly Glu Asp Phe Glu Lys Leu Gln Lys Asn Val Thr Ser Lys 
    210                 215                 220 

Gly Gly Thr Thr His Glu Ala Val Glu Ala Phe Arg Arg His Arg Val 
225                 230                 235                 240 

Ala Glu Ala Ile Ser Glu Gly Val Cys Ala Cys Val Arg Arg Ser Gln 
                245                 250                 255 

Glu Met Glu Arg Gln Tyr Gln 
            260 

 
           
             21  
             132  
             DNA  
             Neisseria meningitidis  
             
               CDS  
               (1)..(132)  
             
           
            21 

atg ggg aaa cgg ctg atg ccg tct gaa ggg aaa ggt gcg gca ggc aat       48 
Met Gly Lys Arg Leu Met Pro Ser Glu Gly Lys Gly Ala Ala Gly Asn 
  1               5                  10                  15 

ccg gag gcg cat aaa aaa gca gat ata ttc gga ctg cac ctc ccg aat       96 
Pro Glu Ala His Lys Lys Ala Asp Ile Phe Gly Leu His Leu Pro Asn 
             20                  25                  30 

ata tct gcc tgc tgt ttc ctc ttt att cag cct tta                      132 
Ile Ser Ala Cys Cys Phe Leu Phe Ile Gln Pro Leu 
         35                  40 

 
           
             22  
             44  
             PRT  
             Neisseria meningitidis  
           
            22 

Met Gly Lys Arg Leu Met Pro Ser Glu Gly Lys Gly Ala Ala Gly Asn 
  1               5                  10                  15 

Pro Glu Ala His Lys Lys Ala Asp Ile Phe Gly Leu His Leu Pro Asn 
             20                  25                  30 

Ile Ser Ala Cys Cys Phe Leu Phe Ile Gln Pro Leu 
         35                  40