Abstract:
Compositions are disclosed which comprise a homopolyamino acid and stereoisomers thereof adsorbed on an insoluble support material. The compositions are useful as catalysts for the stereoselective epoxidation of olefins.

Description:
CROSS-REFERENCE TO RELATED APPLICATION 
     This application claims priority from German Application No. 198 55 859.7, filed on Dec. 3, 1998, the complete disclosure of which is hereby incorporated herein by reference. 
     BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates to novel supported, diastereomer-enriched and enantiomer-enriched homopolyamino acids, to a process for the production thereof, to the use thereof in a process for the enantioselective epoxidation of C═C double bonds and to preferred intermediates. 
     2. Background Information 
     Enantioselective epoxidation reactions are important reactions for the synthesis of chiral intermediates for organic synthesis. In particular, asymmetrical epoxidation of allyl alcohols according to Sharpless et al. and manganese-salen mediated enantioselective epoxidation according to Jacobsen et al. are well established in synthetic organic chemistry for the synthesis of chiral molecules (Sharpless et al.,  J. Am. Chem. Soc . 1980, 102, 5974 ; J. Am. Chem. Soc . 1987, 109, 5765 ; J. Org. Chem . 1986, 51, 1922; Jacobsen et al.,  J. Am. Chem. Soc . 1990, 112, 2801 ; J. Am. Chem. Soc . 1991, 113, 7063). 
     Another possibility for the asymmetric epoxidation of C═C double bonds has been discovered in the reaction of chalcones with hydrogen peroxide in the presence of enantiomer-enriched polyamino acids (Colonna et al.,  Org. Synth.; Mod. Trends , Proc. IUPAC Symp. 6th, 1986, 275; Julia et al.,  Angew. Chem., Int. Ed. Engl ., 1980, 19, 929). 
     The synthesis methods just stated all have the disadvantage that they are applicable to a relatively narrowly restricted range of substrates. On the basis of this fact and the continuing research activities in this area, it may be concluded that there is a need to discover further improved epoxidation processes. 
     To date, two different variants of the Julia/Colonna epoxidation reaction are known in the prior art, namely the two-phase and three-phase variants (S. M. Roberts et al.  Chem. Commun . 1998, 1159; WO 96/33183). The two-phase variant makes use of an organic solvent and operates with oxidizing agents soluble in these solvents in the presence of the insoluble homopolyamino acids. The three-phase variant also makes use of water as the third phase in addition to the water-insoluble organic solvent. Water-soluble oxidizing agents may accordingly advantageously be used for the reaction, optionally in the presence of phase transfer catalysts. 
     However, it is clear from the last-stated publications relating to epoxidation reactions that the low space-time yields (reaction times of the order of days) and the sometimes poor ee values for many substrates are major deficiencies of these epoxidation methods with regard to their use in an industrial process. 
     On the other hand, it has been found that using immobilization techniques for enzyme mediated reactions may be advantageous with regard, for example, to the recoverability of the catalyst and raising the optical and chemical yield (EP 0 799 894 A2 and  Tetrahedron Asymmetry 1991, 2, 931). 
     SUMMARY OF THE INVENTION 
     In the light of the prior art stated and discussed above, the object of the invention was accordingly to provide supported, diastereomer-enriched and enantiomer-enriched homopolyamino acids which allow the epoxidation of C═C double bonds in the presence of an oxidizing agent. In comparison with prior art supported catalysts, it was the intention that the novel compounds should in particular exhibit a higher rate of reaction combined with better chiral induction. Another object of the invention was to be able to produce the supported catalysts in the simplest and thus lowest cost manner possible and to ensure better handling characteristics with regard to recovery of the catalyst after the reaction, so resulting in advantages for use on an industrial scale. 
     These and further objects which are not stated in greater detail but may be derived in an obvious manner from the prior art are achieved by homopolyamino acids which are adsorbed on an insoluble support material. Preferably the homopolyamino acids have a chain length of 5 to 100 amino acids, more preferably 7 to 50. Homopolyamino acids from the group polyneopentylglycine, polyleucine, polyisoleucine, polyvaline, polyphenylalanine and polyalanine are preferred. In one preferred embodiment, the homopolyamino acids are crosslinked together or are enlarged by organic polymers. Preferred as support material for the homopolyamino acids are compounds containing silicon oxide, nitrocellulose, cellulose or activated carbon. The ratio of homopolyamino acid to support material is preferably between 1:7 and 2:1 parts by weight, more preferably between 1:1 and 1:4 parts by weight. 
     Because diastereomer-enriched and enantiomer-enriched homopolyamino acid is adsorbed on an insoluble support material, catalysts for enantioselective epoxidation are obtained which are extremely simple and thus low in cost to produce and furthermore, utterly surprisingly, are capable of considerably raising the rates of reaction in this reaction in comparison with prior art catalysts. Increased yield and enantiomer excesses in the epoxide products are simultaneously unexpectedly achieved. Furthermore, the supported homopolyamino acids according to the invention may be recycled very effectively and, by virtue of the enlargement and heterogenization thereof, such catalysts have very good handling characteristics on the industrial scale. 
     The supported catalysts may be produced using various diastereomer- and enantiomer-enriched homopolyamino acids. Preferably, however, homopolyamino acids from the group comprising polyneopentylglycine, polyleucine, polyisoleucine, polyvaline, polyalanine and polyphenylalanine are used. Of this group, polyneopentylglycine is the most highly preferred. 
     The chain length of the polyamino acids should be selected such that, on the one hand, chiral induction in the reaction is not impaired and, on the other, the costs for synthesizing the polyamino acids do not rise excessively. The chain length of the homopolyamino acids is preferably between 5 and 100, preferably 7 to 50, amino acids. A chain length of 10 to 40 amino acids is very particularly preferred. 
     A further preferred embodiment is that in which the homopolyamino acids are crosslinked with polyfunctional amines or are enlarged by other organic polymers. Crosslinking agents which are advantageously used are amines, such as for example 1,3-diaminopropane, 1st generation propyleneiminetetraamine dendrimers or crosslinked hydroxy- or aminopolystyrene. Polyethylene glycol/polystyrene based nucleophiles are preferably considered as polymer enlargers. Polyamino acids modified in this manner are described in  Chem. Commun . 1998, 841 et seq., 1159 et seq. and  Tetrahedron Asymmetry  1997, 8, pages 3165 et seq. 
     The insoluble support materials are those preferably synthesized on the basis of silicon oxide, such as for example molecular sieve, silica gel or zeolites together with Celite 521® or Celite Hyflo Super Cell®, Wessalith® Day P. Silica gels having defined pore sizes, such as for example CPC I or CPC II are also advantageous. Sugar derivatives such as nitrocellulose, cellulose or activated carbon are also preferred as support material. 
     The ratio of support material to polyamino acid is determined by two limits. On the one hand, only a certain quantity of polyamino acid may be adsorbed on the insoluble support, while on the other, chiral induction diminishes at a ratio of below 10 wt. % of polyamino acid to support. The ratio of homopolyamino acid to support material is preferably between 1:7 and 2:1 parts by weight, particularly preferably between 1:1 and 1:4 parts by weight. 
     The present invention also provides a simple but extremely advantageous process for the production of the homopolyamino acids, which is distinguished in that a mixture of homopolyamino acid and support material is suspended in an organic solvent and, after filtration, the residue is dried. 
     Another aspect of the present invention is the use of the supported homopolyamino acids in the process for the enantioselective epoxidation of C═C double bonds. The catalysts according to the invention are furthermore preferably used in a process which is performed in an apparatus which is capable of retaining only the catalyst. This apparatus preferably comprises an enzyme membrane reactor (C. Wandrey in Enzymes as Catalysts in Organic Synthesis, ed. M. Schneider, Dordrecht Riedel 1986, 263-284). Another preferred apparatus is a simple fixed bed reactor, such as for example a chromatography column. 
     A further aspect of the invention concerns polyneopentylglycine, which is preferably in a diastereomerically and enantiomerically pure form. 
     Providing polyneopentylglycine as a supported catalyst for the enantioselective epoxidation of substrates containing C═C double bonds unexpectedly makes it possible to achieve a substantial improvement in chiral induction during the epoxidation reaction in comparison with prior art homopolyamino acids (PLL has become established as the best catalyst for this reaction and is commercially available). It has moreover completely surprisingly been established that a more highly quantitative yield is obtained with a shorter reaction time. 
     Reference is made to the above-stated details relating to the homopolyamino acids with regard to chain length, crosslinking and polymer enlargement. 
     Polyneopentylglycine is preferably produced from the N-carboxylic anhydrides (NCAS) of neopentylglycine by nucleophilically initiated polymerization in an analogous manner to homopolyamino acids known in the prior art. 
     Polyneopentylglycine may be used in a process for the production of enantiomer-enriched epoxides. It is furthermore very particularly preferred to use homopolyneopentylglycine in a process for the production of supported epoxidation catalysts of the present invention. 
     As mentioned, the homopolyamino acids to be used in the epoxidation may be produced using prior art methods (c.f. for example Flisak et al., J. Org. Chem. 1993, 58, 6247). The method should be applied to both optical antipodes of the amino acids. Using a specific antipode of a polyamino acid correlates with the stereochemistry of the epoxide, i.e. a poly-L-amino acid gives rise to the optical antipode of the epoxide which is obtained with a poly-D-amino acid. 
     It has been found that treating the homopolyamino acid before the use thereof with a basic, aqueous medium, as described in EP 0 403 252 A2, brings about a further reduction in the rate of reaction in the epoxidation reaction. 
     Adsorption of the homopolyamino acid on the support material advantageously proceeds by 48 hours&#39; stirring in an organic solvent, such as for example in ethers such as THF, in the presence of the support material. The solid material is then filtered out and dried. 
     In the present reaction according to the invention, the process is generally performed in that the homopolyamino acid or the modified derivatives, such as homopolyamino acid which has been crosslinked, polymer enlarged, or adsorbed on insoluble supports, is/are suspended in the reaction solvent mixture to be used, then the oxidizing agent is added, the pH value optionally adjusted and the substrate is added. The order of the process steps need not necessarily be followed, but the oxidizing agent should preferably be added to the mixture last, in order to avoid unintentional, uninduced epoxidation at the beginning of the reaction before addition of the catalyst, as this gives rise to lower ee values in the product. The processes are described in the prior art (S. M. Roberts et al.,  Tetrahedron: Asymmetry  1997, 8, 3163-3173 , J. Chem. Soc., Perkin Trans . 1, 1996, 4, 343-348 ; Chem. Commun . 1998, 1159-1160 and literature cited therein; WO 96/33183; EP 0 403 252). The polyneopentylglycine or supported catalysts according to the present invention are used by simply replacing the polyamino acids used in the described processes with the novel catalyst. It should be noted that the catalysts according to the invention provide good results with both the two-phase and three-phase variants. 
     For the purposes of the invention, however, the two-phase variant is preferred for the supported homopolyamino acids. 
     The superiority of the supported catalysts over unsupported catalysts is illustrated by the following table 1. 
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 1 
               
             
             
               
                   
               
               
                 (see Example 1.a) 
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
           
               
                   
                   
                 t 
                 Conversion 
                 ee value 
               
               
                 No. 
                 Support material 
                 [min] 
                 [%] 
                 [%] 
               
               
                   
               
             
          
           
               
                 1 
                 — 
                 120 
                 43 
                 93 
               
               
                 2 
                 Silica gel 
                 30 
                 99 
                 95 
               
               
                 3 
                 CPC I 
                 35 
                 99 
                 97 
               
               
                 4 
                 CPC II 
                 30 
                 76 
                 95 
               
               
                 5 
                 Molecular sieve 
                 120 
                 93 
                 95 
               
               
                   
               
             
          
         
       
     
     The loading of the support material with homopolyamino acids has an influence on the quality of epoxidation (Table 2). 
     
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 2 
               
             
             
               
                   
               
               
                 (see Example 1.b) 
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
           
               
                   
                 PLL/silica gel 
                   
                   
                   
               
               
                 No. 
                 ratio 
                 t [min] 
                 Conversion [%] 
                 ee value [%] 
               
               
                   
               
             
          
           
               
                 1 
                 1:1 
                 30 
                 97 
                 94 
               
               
                 2 
                   1:3.4 
                 30 
                 99 
                 95 
               
               
                 3 
                 1:7 
                 210 
                 72 
                 93 
               
               
                 4 
                  1:10 
                 210 
                 47 
                 93 
               
               
                   
               
             
          
         
       
     
     Further Examples demonstrating the advantages of the supported homopolyamino acids are shown below (Table 3). 
     
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 (see Example 1.c) 
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
           
               
                   
                   
                   
                 Conversion 
                 ee value 
                   
               
               
                 No. 
                 Cat. 
                 t [h] 
                 [%] 
                 [%] 
                 R,R′ 
               
               
                   
               
             
          
           
               
                   1 
                 PLL 
                 24 
                 50 
                 93 
                 o-NH 2 —Ph,  Ph 
               
               
                 2 
                 PLL/ 
                 3 
                 85 
                 93 
                 o-NH 2 —Ph, Ph 
               
               
                   
                 support 
               
               
                 3 
                 PLL 
                 26 
                 56 
                 89 
                 iPr, Ph 
               
               
                 4 
                 PLL/ 
                 14 
                 78 
                 93 
                 iPr, Ph 
               
               
                   
                 support 
               
               
                   
               
             
          
         
       
     
     The reaction mixtures are worked up using methods known to the person skilled in the art. The soluble epoxide is advantageously separated from the catalyst by filtration and then worked up in aqueous form. If desired, the epoxide may subsequently be purified chromatographically on silica gel. 
     The filterability of the supported catalysts is distinctly improved in comparison with unsupported catalysts. Homopolyamino acids assume the form of pastes, which clog the filter, whereas the supported counterparts are solids and may be separated with simple filtration apparatus. 
     This has positive effects primarily on reuse of the supported catalysts according to the invention. Catalyst losses on repeated use (the No. column indicates the number of uses) are substantially reduced in comparison with unsupported catalysts (Table 6). 
     
       
         
               
             
               
               
               
             
               
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
               
             
           
               
                 TABLE 6 
               
             
             
               
                   
               
               
                 (see Example 1.d) 
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
           
               
                   
                          Supported PLL 
                 Unsupported PLL 
               
             
          
           
               
                 No. 
                 t 
                 Yield 
                 ee 
                 Cat. 
                 t 
                 Yield 
                 ee 
                 Cat. 
               
               
                 1 
                 [min] 
                 [%] 
                 [%] 
                 loss [%] 
                 [min] 
                 [%] 
                 [%] 
                 loss [%] 
               
               
                   
               
             
          
           
               
                 2 
                 200 
                 96 
                 93 
                 3 
                 60 
                 48 
                 97 
                 10 
               
               
                 3 
                 115 
                 90 
                 95 
                 3 
                 60 
                 82 
                 97 
                 7 
               
               
                 4 
                 95 
                 94 
                 95 
                 7 
                 60 
                 81 
                 98 
                 8 
               
               
                 5 
                 105 
                 97 
                 98 
                 2 
                 60 
                 79 
                 97 
                 4 
               
               
                 6 
                 100 
                 91 
                 95 
                 1 
                 150 
                 82 
                 97 
                 8 
               
               
                 7 
                 110 
                 95 
                 98 
                 2 
                 &gt;240 
                 80 
                 96 
                 15 
               
               
                   
               
             
          
         
       
     
     The above results demonstrate the advantage of using the homopolyamino acids in supported form over using them in unsupported form with regard to activity, selectivity, handling and recyclability. This is achieved solely by simply adsorbing the homopolyamino acids on insoluble support materials, which seems extremely surprising yet consequently all the more advantageous. 
     Results of the epoxidation reactions of PLL-CLAMPS are compared below with those for PLN-CLAMPS. PLL means poly-L-leucine, PLN means poly-L-neopentylglycine. All results were measured by chiral HPLC (see Example 2). 
     
       
         
               
             
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 7 
               
             
             
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
           
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
           
               
                   
                        Conditions 
                 Time [h] 
                 Conversion [%] 
                 ee value [%] 
               
               
                   
                   
               
             
          
           
               
                   
                 2-phase/PLL 
                 18 
                 90 
                 95 
               
               
                   
                 2-phase/PLN 
                 6 
                 98-100 
                 &gt;95 
               
               
                   
                 3-phase/PLL 
                 0.5 
                 90 
                 95 
               
               
                   
                 3-phase/PLN 
                 0.5 
                 90 
                 96 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
             
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 8 
               
             
             
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
           
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
           
               
                   
                        Conditions 
                 Time [h] 
                 Conversion [%] 
                 ee value [%] 
               
               
                   
                   
               
             
          
           
               
                   
                 2-phase/PLL 
                 22 
                 95 
                 94 
               
               
                   
                 2-phase/PLN 
                 3 
                 90 
                 98 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
             
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 9 
               
             
             
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
           
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
           
               
                   
                        Conditions 
                 Time [h] 
                 Conversion [%] 
                 ee value [%] 
               
               
                   
                   
               
             
          
           
               
                   
                 2-phase/PLL 
                 8 
                 83 
                 95 
               
               
                   
                 2-phase/PLN 
                 2 
                 93 
                 &gt;95 
               
               
                   
                   
               
             
          
         
       
     
     
       
         
               
             
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 10 
               
             
             
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
             
          
           
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
               
               
                   
               
             
          
           
               
                   
                        Conditions 
                 Time [h] 
                 Conversion [%] 
                 ee value [%] 
               
               
                   
                   
               
             
          
           
               
                   
                 2-phase/PLL 
                 32 
                 60 
                 60 
               
               
                   
                 2-phase/PLN 
                 7 
                 80 
                 75 
               
               
                   
                   
               
             
          
         
       
     
     As is clearly evident from these examples, poly-L-neopentylglycine also has distinct advantages over known prior art homopolyamino acids with regard to activity and selectivity. 
     Using the homopolyamino acids and modified derivatives presented here thus, on the one hand, gives rise to better ee values on epoxidation and, on the other, distinctly reduces reaction times for this transformation. This could not straightforwardly be derived from the prior art. While, as stated above, it is indeed known to absorb enzymes on insoluble supports, it is not consequently in the least obvious that the compounds according to the invention will be improved by such a measure. On the contrary, the person skilled in the art could have expected that adsorption of the polymer on the solid support material would bring about a conformational reorientation and immobilize this conformation such that the chiral induction thereof would consequently be impaired. Since the mechanism of the stated epoxidation reaction is not yet known, it was extremely surprising that the described immobilization should bring about an increase in activity and induction and not the opposite. 
     Moreover, the possibility of binding enzymes to insoluble supports does not make it in the least obvious that the homopolyamino acids at issue in the present case may be similarly firmly adsorbed on the insoluble support materials. Enzymes are macromolecules, which, in comparison with the homopolyamino acids of the present invention, may enter into quite different non-covalent interactions with substrates. 
     In comparison with the chemically modified, polymer enlarged homopolyamino acids, the production of the supported homopolyamino acids by physical adsorption is so straightforward and thus low in cost that the use thereof is preferred on the industrial scale. It is furthermore advantageous that the handling properties and recoverability are distinctly improved in comparison with unsupported homopolyamino acids, as the heterogenization results in better filterability. Standard prior art homopolyamino acids are pasty and capable of clogging the pores of filters and membranes, which would cause considerable difficulties, especially for the industrial use thereof. The epoxidation catalysts according to the invention are accordingly ideally suitable for industrial use in advantageous apparatus such as the fixed bed reactor and enzyme membrane reactor (C. Wandrey in Enzymes as Catalysts in Organic Synthesis, ed. M. Schneider, Dordrecht Riedel 1986, 263-284), which permit continuous or semi-continuous performance of the reaction, which is highly preferred for an industrial process. 
     This invention accordingly for the first time makes the use of the catalysts according to the invention in an industrial process appear economically advantageous. 
     The person skilled in the art takes the term homopolyamino acids to mean polymers of amino acids from a single source. For the purposes of the invention, however, the homopolyamino acids used may also comprise copolymers of various amino acids, which, however, have domains determining chiral induction which should consist of uniform amino acids. The term homopolyamino acids accordingly also refers to polymers synthesized from heterochiral amino acids. It is again the case that the domains which determine chiral induction should consist of a stereochemically uniform sequence of amino acids. 
     Supported is taken to mean the physical adsorption of molecules on the support materials according to the invention. 
     For the purposes of the invention, the term enantiomer-enriched should be taken to mean the mixture of an enantiomer with the optical antipode thereof in a proportion ranging between &gt;50% and &lt;100%. 
     For the purposes of the invention, the term diastereomer-enriched is taken to mean the mixture of an diastereomer with the other diastereomer thereof in a proportion ranging between &gt;50% and &lt;100%. 
    
    
     DETAILED DESCRIPTION OF THE INVENTION 
     EXAMPLES 
     1) Use of Supported PLL as Catalyst 
     Standard conditions for catalyst testing (apply to all supported catalysts, unless otherwise stated). 
     Catalyst production: 70 mg of PLL (1,3-diaminopropane used as polymerization initiator,  Chem. Commun . 1998, 1159; S. M. Roberts et al.  J. Chem. Soc. Perkin Trans. I , 1998, 3171) are stirred with 240 mg of support material in 2 ml of THF for 48 h using a magnetic stirrer. The suspension is then filtered and the residue dried at 1.1 kPa and RT for 1 h, then at 50-55° C. and 0.008 kPa for 7 h. 
     Epoxidation reaction: 18 mg of trans-chalcone, 10 mg of UHP (urea peroxide compound), 155 mg of supported catalyst and 15 μl of DBU are stirred in 1 ml of THF at RT. Conversion and ee value are determined by chiral HPLC. 
     HPLC conditions: Chiral HPLC was performed using Chiralpak AD columns. To this end, an aliquot of the reaction mixture was taken and, once filtered, analysed at 254 nm at a flow rate of 1.00 ml/min with 10 wt. % EtOH/hexane as mobile solvent. 
     1.a) Various Supports 
     Silica gel=Silica gel 60 (230-400 mesh, Merck) 
     CPC I=controlled pore size carrier (375 Å, 30-45 mesh, Fluka) 
     CPC II=controlled pore size carrier, silane coated, derived from the 3-aminopropyl residue (375 Å, 30-45 mesh, Fluka), 
     Celithe 521® (Aldrich), Celithe Hyflo Super Cell® (Fluka), Wessalith® Day P.=SiO 2 , 
     Molecular sieve (SiO 2 , batch: MPM TI, Degussa) 
     Zeolite TS1 (5.5 Å, 3% Ti). 
     Reaction Conditions for PLL Use: 
     Similar to literature methods—two-phase variant. 
     Conversions and ee values were determined by chiral HPLC. 
     Retention times of the enantiomeric epoxides: 15.9 min (main enantiomer) and 23.7 min. 
     In entry 1, support materials and homopolyamino acid were combined only just before epoxidation. 
     1.b) Differing Loading 
     Supported catalysts with the corresponding polyamino acid/support ratios were otherwise produced in accordance with the standard process. Silica gel was used as the support material. 
     Conversion and ee value were detected by chiral HPLC. Retention times, see Example 1.a). 
     1.c) Variation in Substrates 
     Support material: silica gel; ratio 1:3.4 
     Reaction conditions for PLL comparison: 
     Similar to literature methods—two-phase variant. 
     Entries 1 and 2: 
     Conversion and ee value were detected by chiral HPLC, 14% EtOH in hexane, 230 nm. 
     Retention times of the enantiomeric epoxides: 25.6 min (main enantiomer) and 31.2 min. 
     Entries 3 and 4: 
     Conversion and ee value were detected by chiral HPLC, 5% EtOH in hexane, 230 nm, flow rate 0.7 ml/min. 
     Retention times of the enantiomeric epoxides: 12.6 min (main enantiomer) and 23.3 min. 
     1.d) Catalyst Recycling 
     Reaction conditions for PLL comparison: Similar to literature methods—two-phase variant (initial quantity of catalyst 2.0 g). 
     Catalyst Recovery: 
     After the reaction, the mixture was filtered, the filter residue washed with water/acetone (1:1), acetone and EtOAc and then dried under an oil pump vacuum. 
     Reaction conditions for use of the supported catalyst: Support material: silica gel; ratio 1:3.4 
     116 mg of trans-chalcone, 65 mg of UHP, 100 μl of DBU were reacted in 6.5 ml of THF at RT with the appropriate quantity of supported catalyst (initial quantity 1.0 g). 
     Catalyst Recovery: 
     After the reaction, the mixture was filtered, the filter residue washed with THF, EtOH and, finally, again with THF. The residue was then dried under the standard conditions. 
     2) Use of Polyneopentylglycine (PLN) as Catalyst 
     Chiral HPLC was performed using Chiralpak AD columns. To this end, an aliquot of the reaction mixture was taken and, once filtered, analysed at a flow rate of 1.00 ml/min. 
     Testing of the substrates revealed the following results: 
     
       
         
               
               
               
               
             
               
               
               
               
             
           
               
                   
               
               
                   
                   
                 R t  [min] 
                   
               
               
                   
                   
                 of the 
               
               
                   
                 Mobile 
                 enantiomeric 
                 λ 
               
               
                 Substrate 
                 phase 
                 epoxides 
                 [nm] 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 10%  EtOH  in hexane 
                 14.0 and 20.5 
                 254 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1%  i-PrOH  in hexane 
                 15.0 and  22.0 
                 230 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 1%  i-PrOH  in hexane 
                 14.0 and  21.5 
                 230 
               
               
                   
               
               
                 
                   
                             
                     
                         
                         
                     
                   
                 
                 10%  EtOH  in hexane 
                 17.3 and  21.1 
                 230 
               
               
                   
               
             
          
         
       
     
     Production of Catalyst (PLN-CLAMPS): 
     A dried 2 l, three-necked flask is filled with 0.1400 mol of neopentylglycine (commercially available) and kept at 100° C. for 24 h under a vacuum. The flask is then purged with nitrogen and the contents combined with 1 l of dry THF. Once the suspension has been heated to approx. 50° C., 0.0513 mol of triphosgene is added dropwise over a period of 15 minutes. After 2 h, the solution is cooled to RT, filtered and evaporated under a vacuum. The crystalline residue is dissolved in a minimum quantity of THF and precipitated by addition of 1 l of n-hexane. The NCA of L-neopentylglycine is obtained in 85% yield as a colourless solid of melting point 129.3-131.2° C.; δ H  (300 MHz; CDCl 3 ) 1.02(9H, s, 3×CH 3 ), 1.65 (1H, dd, J 14.7 and 9.9, β-CH 2 ), 2.0 (1H, dd, J 14.7 and 2.4, β-CH 2 ), 4.38 (1H, dd, J 9.9 and 2.4, α-CH), 6.8 (1H, br, NH). 
     0.1180 mol of L-neopentylglycine NCA is combined in 250 ml of THF with (3.668 mol) of CLAMPS (crosslinked aminopolystyrene) and the mixture was stirred 5 days under nitrogen at RT. The suspension is then filtered and the filtrate is treated with water for 30 min, then with acetone/water (1:1), acetone/water (4:1), acetone (2×), EE (2×), diethyl ether (2×) and then dried under a vacuum. 
     The polymer is then activated for several hours in a mixture of toluene/4 M sodium hydroxide solution (2.5:1). 
     PLL-CLAMPS may be produced in an analogous manner (c.f. also Chem. Commun. 1998, 1159). 
     Epoxidation: 
     3-phase variant 
     100 mg of the polymer (PLL-CLAMPS or PLN-CLAMPS) in 0.8 ml of toluene and 0.2 ml of 4 M NaOH (12 eq.) are combined at 0° C. in an ice bath with 0.2 ml of 30% aqueous H 2 O 2  (21 eq.) and kept at this temperature for 6 h. The enone (1 eq.), a further 0.5 ml of H 2 O 2  and 0.2 ml of toluene are added to the mixture. Samples are taken after various periods of time and analysed by chiral HPLC. 
     2-phase variant 
     100 mg of the polymer (PLL-CLAMPS or PLN-CLAMPS) and 0.24 mmol of the enone in 0.8 ml of THF are combined with 0.36 mmol (1.5 eq.) of DBU and 0.28 mmol of UHP (urea hydrogen peroxide compound). The mixture is stirred at RT. Samples are taken after various periods of time and analysed by chiral HPLC. 
     Publications cited herein are hereby incorporated by reference in their entirety.