Abstract:
Disclosed are hydrophilic polymers such as polysaccharides, including hyaluronic acid of any origin, modified by reaction with epoxy-functional-silicones. Hydrophobic silicon, which contains chemically active groups covalently attach to the backbone of the hydrophilic polymer and gives these new, modified polymers the ability to dissolve hydrophobic compounds including oils, drugs, and vitamins, while maintaining the hydrophilic properties and benefits of the unmodified polymer. With respect to topical applications these polymers substantially increase the stability of formulations and provide for ease of preparation. The properties and advantages of the original polymer are maintained while other properties are augmented, namely the ability to combine with or dissolve hydrophobic and hydrophilic drugs. The products can be used alone or in combination with other substances for various applications including cosmetic, medical, and drug delivery applications. Also disclosed are methods for preparing them.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
       [0001]    Not Applicable 
       STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT 
       [0002]    Not Applicable. 
       THE NAMES OF THE PARTIES TO A JOINT RESEARCH AGREEMENT 
       [0003]    Not Applicable 
       INCORPORATION-BY-REFERENCE OF MATERIAL SUBMITTED ON A COMPACT DISC 
       [0004]    Not Applicable 
       REFERENCE TO A MICROFICHE APPENDIX 
       [0005]    Not Applicable 
       BACKGROUND OF THE INVENTION 
       [0006]    1. Field of the Invention 
         [0007]    The present invention relates to modified hydrophilic polymers such as hyaluronic acid, also known as hyaluronan and mixtures thereof, containing hydrophobic silicone groups prepared by reaction of the hydrophilic polymer with epoxy-functional-silicones, their preparation and compositions containing them. 
         [0008]    2. Description of Related Art 
         [0009]    Relevant prior art includes the following references: U.S. Pat. No. 7,521,434 discloses cross-linked gels of hyaluronic acid with hydrophobic polymers and processes; Mensitieri et al. Journal of Material Science; Materials in Medicine (1994) pages 743-747; The rheological behavior of animal vitreus and its comparison with vitreal substitutes; and Schwarz, K. Proc Nat Acad Sci (1973) Vol 70 pp 1608-1612; A Bound form of Silicon in Glycosaminoglycans and Polyuronides. 
         [0010]    Hyaluronic acid (HA) and its derivatives are important materials used in the medical and cosmetic industry. Their unique viscoelastic properties combined with their high water binding properties and exceptional biocompatibility have led to a wide variety of products in the ophthalmic, arthritis, wound healing, anti-adhesion, drug delivery, soft tissue augmentation, and burn management fields, as well as use in topical cosmetic moisturization. It is an excellent biomaterial for a variety of combined uses the medical field due to its viscoelastic and biocompatibility properties. The water binding and hydration properties of hyaluronic acid provide water to the skin. This moisturizing effect is widely used in the cosmetic industry. 
         [0011]    Hyaluronic acid is a naturally occurring polysaccharide that consists of alternating N-acetyl-D-glucosamine and D-glucuronic acid monosaccharide units linked with alternating [beta] 1-3 glucoronidic and [beta] 1-4 glucosaminidic bonds. The molecular weight of hyaluronic acid is generally within the range of 50,000 up to more than 8,000,000 
         [0012]    Hyaluronic acid is found in all vertebrates—in the skin, vitreous humor, the synovium, the cartilage, and the umbilical cord. It is not species specific and therefore highly biocompatible. It is known for its efficiency of hydration, it has a binding capacity of up to 20 times its weight in water and it is the natural moisturizer in the cell matrix of the skin. It typically exists at concentrations of 200 micrograms per gram of dermal tissue. Cross-linking of HA has been used to improve the physical properties of the molecule and to enhance its properties for various uses, or to immobilize the HA to various supports for medical purposes including the diagnosis of male infertility. Formulations of hyaluronic acid have been used by the cosmetic industry as skin moisturizers. One important use of hyaluronic acid and its derivatives is founded in its properties as a drug delivery vehicle. Various drug and other biological actives have been loaded into hydrogels of HA and its derivatives; however there are some limitations on the types of substances capable of being loaded based on their chemical nature. Hydrogels composed largely of water have difficulty accepting substances of hydrophobic nature. Some organic solvents used to solubilize a hydrophobic substance will dehydrate the hydrogel or precipitate the HA. Some methods have been developed to overcome this problem although there is still difficulty with some substances. Hyaluronic acid has been used for delivery of drugs such as diclofenac. 
         [0013]    Silicones are also a very versatile class of compounds used for many applications in the medical field and cosmetic industry. Topical application of silicones have been used for wound healing, drug delivery and burn treatment and are also topically used in cosmetic formulations. Silicones are in general, hydrophobic and their moisturizing properties are due to their ability to provide a vapor barrier to the skin. 
         [0014]    Silicone Gels are also used for delivery purposes in the medical field and the cosmetic industry. Their hydrophobic nature makes them excellent vehicles for incorporating hydrophobic substances. Silicone implants are used to deliver contraceptives and other hydrophobic drugs. 
       BRIEF SUMMARY OF THE INVENTION 
       [0015]    The invention provides, in one aspect thereof, chemically modified products of hydrophilic polymers and one or more silicone containing compounds. The hydrophilic polymer may be a natural or synthetic polysaccharide, including heparin, heparin sulfate, chondroitin, chondroitin sulfate, hyaluronic acid, hydroxyethyl cellulose, carboxymethyl cellulose and an alginates. The compositions of the invention may also contain one or more hydrophobic substances, including oils, silicones, sun screens, antibiotics and steroids. 
         [0016]    In another aspect, the invention provides methods for making the modified products by subjecting them to reaction with an epoxy functionalized silicone compound in an aqueous alkaline solution at a temperature of about 20° C. The HA concentration before reaction with the epoxy compound may range from 20% to 0.01%, preferably about 3%. The molar ratio of the epoxy silicone compound to HA may range from 1:20 to 4:1, and preferably about 2:1. It should be noted that excess epoxy silicone can be used to drive the reaction forward. By this is meant that even though at a given range of a 4:1, the molar ratio is actually in excess of the needs at reactive sites, one can continue to add more reactive epoxy which will affect of kinetics, i.e., driving the reaction forward, even though the amount of reactant that will react is limited. 
         [0017]    The compositions of the present invention combine the advantages of both hyaluronic acid and silicone gels in that they contain both hydrophobic and hydrophilic moieties. The modified hyaluronic acid has the ability to incorporate both large volumes of water and hydrophilic substances as well as hydrophobic substances such as oils, sun screens, vitamins, antibiotics, steroids and other drugs. Thus, there is provided an improved drug delivery vehicle. The nature of the material also provides a composition which is hydrophobic in nature and is thus easier to formulate in a cosmetic composition. 
     
    
     DETAILED DESCRIPTION OF THE INVENTION 
       [0018]    The present invention provides for the preparation of compositions of chemically modified polysaccharides prepared in the presence of an epoxy functionalized silicone compound, wherein the ratio of the silicone compound to the polysaccharide is 1:20 to 4:1, and the concentration of the polysaccharide in the aqueous solution is from 0.05 to 100 mg/ml. 
         [0019]    The present invention relates to compositions prepared by the process of incorporating the silicone compound through an epoxy functionalized silicone compound and conducting a chemical reaction in the presence of the polysaccharide such as hyaluronan (HA). 
         [0020]    The present invention provides compositions of polysaccharide products prepared with an epoxy functionalized silicone compound in the family of epoxy functionalized silicones such as 2 (3,4 epoxycyclohexyl)ethyltrimethoxysilane, epoxypropoxypropyl terminated polydimethylsiloxane, tris (glycidoxypropyldimethylsiloxy) phenylsilane, mono-(2,3-epoxy) propylether terminated polydimethylsiloxane-120 cSt, epoxycyclohexylethyl terminated polydimethylsiloxane, 25-35 cSt and glycidoxypropyltrimethoxysilane. 
         [0021]    The products of the invention are prepared by reacting the epoxy silicone compound or compounds in the presence of the polysaccharide such as hyaluronan (HA) to obtain a modified polysaccharide. More than one epoxy functionalized silicone can be used, each having a variety of properties that contribute to the final product; for example by changing the hydrophobic-hydrophilic nature, contributing to the charge of the molecule, or the final viscosity of the product. 
         [0022]    The silicon compound incorporated may also be a group capable of undergoing further polymerization or reactions with new functional groups such as trialkoxysilanes, dialkoxysilanes, monoalkoxysilanes, and vinyl groups, added, allowing further modification of the product. 
         [0023]    The present invention provides for a material that can be used alone or with other substances which may be added to enhance the properties for a particular purpose. These products may be added or covalently bound substances that can be applied for numerous applications including, for the use in cosmetic, medical, and drug delivery fields. 
         [0024]    The present invention provides for a biocompatible material, such materials which may be useful as a stable form of implants. HA implants have limited duration since natural mechanisms for the clearance from the body; a more resistant implant can improve the efficacy and durability of devices used for soft tissue augmentation, drug delivery, anti-adhesion, ophthalmic, and anti-arthritis. 
         [0025]    The present invention provides for a topical composition with a hydrating effect on the skin. Hyaluronic acid provides a high water binding capacity, typically 20 grams of water per gram of hyaluronic acid, such a composition will provide a high degree of moisturization to the skin. Compositions of hyaluronic acid have been used to promote wound healing and reduce scaring. Silicone gels and semi-occlusive membranes are commercially available, e.g., Cica Care Silicone Gel Dressing Sheeting—by Cica Care, ScarAway Professional Grade Silicone Scar Treatment Sheets by Mitchell-Vance Laboratories and ScarErase by ScarErase. Inc. for the treatment of chalets and hypertrophic scars. It is believed that silicon membranes create a barrier impermeable to water thereby creating a moisturization effect reducing scarring. 
         [0026]    The present invention is based on hyaluronic acid, a naturally occurring polysaccharide in the human body. It is a common constituent in the tissues and is highly biocompatible. Since the molecule is not species specific it is not antigenic and therefore non-immunogenic. The normal content of hyaluronic acid in the skin is 0.2 mg/gram of tissue, although since it is limited to the extracellular space the concentration of HA in the fluid of the extracellular space is actually about 2.5 mg/ml in concentration. It can therefore be appreciated that the present invention will be a non-inflammatory non-irritating biologically acceptable treatment. 
         [0027]    The following examples will further illustrate the invention in more detail. 
       EXAMPLES 
     Example 1 
       [0028]    4.00 gms of sodium hyaluronate (HA from viable hemolytical streptococci, Molecular Weight 1.93 million.) were mixed for one day with 96 gms of deionized water to give a 4.0% solution of HA. 7.5 ml of 2.0 M NaOH were then added to the mixture and stirred on a Silverson High Shear Mixer, for 1 minute using a low-shear-head at 400 rpm. Then, 2.5 gms of 2(3,4 epoxycyclohexyl)ethyltrimethoxysilane were added. The sample was mixed for 1 minute using a low-shear-head at 400 rpm on a Silverson High Shear Mixer. The pH was adjusted to 12 by the addition of 2.0% HCl while mixing. The reaction was allowed to continue for four hours before neutralizing the reaction mixture by the addition of 70 ml 2.0% HCl. The final volume was 182.00 gms. The product was washed in dialysis tubing against 2 kg of distilled water, and the water was exchanged 5 times. 182.00 gms of a creamy white product were formed. 
       Example 2 
       [0029]    15 gms of sodium hyaluronate (HA from viable hemolytical streptococci, Molecular Weight 1.93 million.) were suspended in 150 ml of methanol. 225 ml of distilled water and 30 ml of 0.2 M NaOH were added to hydrate the HA. Then, 7.5 gms of 2(3,4 epoxycyclohexyl)ethyltrimethoxysilane were added and stirred for 15 minutes. 150 ml of 50% methanol in water were added and stirring continued. After 30 min the pH was reduced to 9 by the addition of 14 ml of 2% HCL. The reaction was continued for 2 hours. The reaction product was neutralized to pH 7 with 2% HCL, and the volume adjusted to 1500 ml by the addition of distilled water. The product was placed into dialysis vs 20 kg of distilled water. The wash water was exchanged 3 times over 2 days. 1626 gms of a creamy white product were formed. 
       Example 3 
       [0030]    This example illustrates the preparation of a modified HA/silicone product prepared under aqueous conditions. 15.00 gms of sodium hyaluronate (Microbial Fermented HA, Molecular Weight 2.07 million.) were mixed for one day with 485 gms of deionized water and the HA was allowed to hydrate overnight to give a 3.0% solution of HA. 42.5 ml of 2.0 M NaOH were then added to the mixture and stirred with a kitchen aid mixer, for 10 minutes stirred at low speed until a smooth consistency was obtained. Then, 12.5 gms of mono-(2,3-epoxy) propylether terminated polydimethylsiloxane-120 cSt were added. The sample was mixed for 45 minutes using a kitchen aid mixer at low speed. The pH was adjusted to 12 by the addition of 2.0% HCl while mixing. The reaction was allowed to continue for two hours before neutralizing by the addition of 355 ml 2.0% HCl. The mixture was brought up to 1 kg with water and was washed in dialysis tubing against 20 L of distilled water. The wash water was exchanged 5 times over 2 days. 1565 gms of a creamy white product having a smooth and silky feel were formed. 
       Example 4 
       [0031]    This example illustrates the preparation of a modified HA/silicone product prepared under aqueous conditions. 15.00 gms of sodium hyaluronate (Microbial Fermented HA, Molecular Weight 2.07 million.) were mixed for one day with 485 gm of deionized waters and allowed the HA to hydrate overnight to give a 3.0% solution of HA. 42.5 ml of 2.0 M NaOH were then added to the mixture and stirred with a kitchen aid mixer for 10 minutes stirred at low speed until a smooth consistency was obtained. Then, 12.5 gms of epoxycyclohexylethyl terminated polydimethylsiloxane, 25-35 cSt were added. The sample was mixed for 45 minute using a kitchen aid mixer at low speed. The pH was adjusted to 12 by the addition of 2.0% HCl while mixing. The reaction was allowed to continue for two hours before neutralizing by the addition of 355 ml 2.0% HCl. The mixture was brought up to 1 kg with water and was washed in dialysis tubing against 20 L of distilled water. The wash water was exchanged 5 times over 2 days. 1187 gms of a creamy white product having a smooth and silky feel was formed. 
       Example 5 
       [0032]    This example illustrates the preparation of a modified HA/silicone product prepared under aqueous conditions. 3.00 gms of sodium hyaluronate (Microbial Fermented HA, Molecular Weight 2.07 million.) were mixed for one day with 97 gms of deionized water and the HA was allowed to hydrate overnight to give a 3.0% solution of HA. 14.5 ml of 0.2 M NaOH were then added to the mixture and stirred at 800 rpm with a Lightnin brand overhead mixer for 10 minutes, stirred at low speed until a smooth consistency was obtained. Then, 0.2 gram of glycidoxypropyltrimethoxysilane (Dow Corning Z-6040) was added. The sample was mixed for 45 minute at 800 rpm with a Lightnin overhead mixer. The pH was adjusted to 12 by the addition of 2.0% HCl while mixing. The reaction was allowed to continue for two hours before being neutralized by the addition of 8 ml 2.0% HCl. The mixture was brought up to 200 ml with water and 150 ml were washed in dialysis tubing against 10 L of distilled water. The wash water was exchanged 5 times over 2 days. 247 gms of a clear transparent product were formed. 
       Example 6 
       [0033]    This example illustrates the preparation of a modified HA/silicone product prepared under aqueous conditions. 15.00 gms of sodium hyaluronate (Microbial Fermented HA, Molecular Weight 2.07 million.) were mixed for one day with 485 gms of deionized water and the HA was allowed to hydrate overnight to give a 3.0% solution of HA. 42.5 ml of 2.0 M NaOH were then added to the mixture and stirred with a kitchen aid mixer, for 10 minute stirred at low speed until a smooth consistency was obtained. Then, 12.5 gms of 2(3,4 epoxycyclohexyl)ethyltrimethoxysilane were added. The sample was mixed for 45 minute with a kitchen aid mixer at low speed. The pH was adjusted to 12 by the addition of 2.0% HCl while mixing. The reaction was allowed to continue for two hours before being neutralized by the addition of 355 ml 2.0% HCl. The mixture was brought up to 1 kg with water and was washed in dialysis tubing against 20 L of distilled water. The wash water was exchanged 5 times over 2 days. 1248 gms of a creamy white product having a smooth and silky feel were formed. The product was preserved with 6.25 gram of phenonip. 
       Example 7 
       [0034]    Five glass vials filled with 20 ml of the sample prepared in Example 2, and placed in an incubator at 37° C. or stored in the dark at room temperature (22° C.). A vial was removed at each of 1, 5, 8, and 12 month time points to monitor changes in appearance, consistency, pH, and viscosity of the product. The viscosity was measured on a Brookfield DV II+ Pro viscometer, Spindle #25 with a small cup adapter and a shear rate of 13.2 sec-1, at 25° C. 
         [0035]    No significant changes in the sample were noted over one year. Only a small decrease was noted in the viscosity, indicating that the product was stable. 
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                 37° C. Stability Study 
               
             
          
           
               
                 Time point 
                   
                   
                   
                   
                   
               
               
                 (months) 
                 Appearance 
                 Consistency 
                 pH 
                 Viscosity 
                 % change 
               
               
                   
               
             
          
           
               
                 0 
                 No change 
                 No change 
                 7.5 
                 2362 
                 0 
               
               
                 1 
                 No change 
                 No change 
                 7.5 
                 2404 
                 +1.8 
               
               
                 5 
                 No change 
                 No change 
                 7.5 
                 2475 
                 +4.8 
               
               
                 8 
                 No change 
                 No change 
                 7.5 
                 2302 
                 −2.5 
               
               
                 12 
                 No change 
                 No change 
                 7.5 
                 2194 
                 −7.1 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
               
               
               
             
               
               
               
               
               
               
             
           
               
                   
               
               
                 22° C. Stability Study 
               
             
          
           
               
                 Time point 
                   
                   
                   
                   
                   
               
               
                 (months) 
                 Appearance 
                 Consistency 
                 pH 
                 Viscosity 
                 % change 
               
               
                   
               
             
          
           
               
                 0 
                 No change 
                 No change 
                 7.5 
                 2362 
                 0 
               
               
                 1 
                 No change 
                 No change 
                 7.5 
                 2491 
                 +5.5 
               
               
                 5 
                 No change 
                 No change 
                 7.5 
                 2487 
                 +5.3 
               
               
                 8 
                 No change 
                 No change 
                 7.5 
                 2364 
                 −0.2 
               
               
                 12 
                 No change 
                 No change 
                 7.5 
                 2358 
                 −7.1 
               
               
                   
               
             
          
         
       
     
       Example 8 
       [0036]    The product Example 6 was sent for a Human Repeat Insult Patch Test to an independent lab in order to determine if the material causes any irritation or allergic reaction. The skin of 50 subjects were tested, 24 hour exposures to the product made in Example 6 three times a week for three consecutive weeks. The skin was evaluated after each application. Following a 10-14 day rest, a retest/challenge dose was applied an evaluated after 48 and 96 hours. The test sites were scored according to standards set by The International Contact Dermatitis Research Group (ICDRG). No adverse reactions of any kind were reported. There were no signs or symptoms of sensitization (contact dermatitis). 
       Example 9 
       [0037]    This example illustrates the preparation of a formulation with a product prepared in Example 2 plus hydrophobic compounds and 49.5% water. 
         [0000]    
       
         
               
               
               
               
             
           
               
                   
               
               
                   
                   
                 Percent  
                 Grams 
               
               
                 Part 
                 Ingredient 
                 by Weight 
                 required 
               
               
                   
               
             
             
               
                 A 
                 product in Example 2 
                 44.0 
                 44.0 
               
               
                   
                 Parsol MCX 
                  2.5 
                  2.5 
               
               
                   
                 Silicone Fluid 200 
                  2.5 
                  2.5 
               
               
                   
                 phenonip 
                  0.5 
                  0.5 
               
               
                   
                 water 
                 49.5 
                 49.5 
               
               
                 B 
                 Carbopol 934 
                  0.5 
                  0.5 
               
               
                 C 
                 Triethanolamine 
                  0.5 
                  0.5 
               
               
                   
               
             
          
         
       
     
         [0038]    44 gms of the product prepared in example 2 were added to the remaining ingredients in part A. The mixture was stirred until completely mixed. Part A and Part B were mixed and placed on a shaker overnight. Triethanolamine was then added to create a thick cosmetic cream. 
       Example 10 
       [0039]    This example illustrates the preparation of a formulation with a product prepared in Example 3 plus hydrophobic compounds and 49.5% water. 
         [0000]    
       
         
               
               
               
               
             
           
               
                   
               
               
                   
                   
                 Percent  
                 Grams 
               
               
                 Part 
                 Ingredient 
                 by Weight 
                 required 
               
               
                   
               
             
             
               
                 A 
                 product in Example 3 
                 44.0 
                 44.0 
               
               
                   
                 Parsol MCX 
                  2.5 
                  2.5 
               
               
                   
                 Silicone Fluid 200 
                  2.5 
                  2.5 
               
               
                   
                 phenonip 
                  0.5 
                  0.5 
               
               
                   
                 water 
                 49.5 
                 49.5 
               
               
                 B 
                 Carbopol 934 
                  0.5 
                  0.5 
               
               
                 C 
                 Triethanolamine 
                  0.5 
                  0.5 
               
               
                   
               
             
          
         
       
     
         [0040]    44 gms of the product prepared in example 3 were added to the remaining ingredients in part A. The mixture was stirred until completely mixed. Part A and Part B were mixed and placed on a shaker overnight. Triethanolamine was then added to create a thick cosmetic cream. 
       Example 11 
       [0041]    This example illustrates the preparation of a formulation with a product prepared in Example 2. 
         [0000]    
       
         
               
               
               
               
             
               
               
               
               
             
           
               
                   
               
               
                   
                   
                 Percent by 
                 Grams 
               
               
                 Part 
                 Ingredient 
                 Weight 
                 required 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 A 
                 product in 
                 79.2 
                 79.2 
               
               
                   
                 Example 2 
                   
                   
               
               
                   
                 Carbopol 934 
                 0.5 
                 0.5 
               
               
                 B 
                 Vitamin E 
                 3.96 
                 3.96 
               
               
                   
                 Parsol MCX 
                 3.96 
                 3.96 
               
               
                   
                 Silicon Quat 
                 11.08 
                 11.08 
               
               
                   
                 Microemultion 
                   
                   
               
               
                 C 
                 Triethanolamine  
                 0.5 
                 0.5 
               
               
                 D 
                 Roseoil W/S 
                 0.3 
                 0.3 
               
               
                   
                 Phenonip 
                 0.5 
                 0.5 
               
               
                   
               
             
          
         
       
     
         [0042]    79.2 gms of the product prepared in example 2 were mixed with Carbopol 934 in part A The mixture was stirred until completely mixed. Part B was prepared by mixing the ingredients in Part B using a lab mixer. Part A and Part B were combined and mixed until uniform. Triethanolamine in Part C was then added to create a thick cosmetic cream. Roseoil W/S and Phenonip in Part D were added. 
       Example 12 
       [0043]    This example illustrates the preparation of a formulation with a product prepared in Example 2 and 44.5% Petrolatum. 
         [0000]    
       
         
               
               
               
               
             
               
               
               
               
             
           
               
                   
               
               
                   
                   
                 Percent by 
                 Grams 
               
               
                 Part 
                 Ingredient 
                 Weight 
                 required 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 1 
                 product in 
                 20 
                 20 
               
               
                   
                 Example 2 
                   
                   
               
               
                   
                 water 
                 20 
                 20 
               
               
                 2 
                 Carbopol 934 
                 0.3 
                 0.3 
               
               
                 3 
                 Petrolatum 
                 44.5 
                 44.5 
               
               
                   
                 Vitamin E acetate 
                 5.0 
                 5.0 
               
               
                   
                 Parsol MCX 
                 7.4 
                 7.4 
               
               
                 4 
                 Triethanolamine 
                 0.3 
                 0.3 
               
               
                   
                 1% Sodium 
                 2 
                 2 
               
               
                   
                 Hyaluronate in 
                   
                   
               
               
                   
                 Water 
                   
                   
               
               
                 5 
                 phenonip 
                 0.5 
                 0.5 
               
               
                   
               
             
          
         
       
     
         [0044]    In part 1, 20 gms of the product prepared in example 2 were added to 20 gms of water and mixed until a uniform viscous solution formed. 0.3 gm of Carbopol 934 was added to the mixture in part 1 and mixed using a lab stirrer for 20 minutes until a uniform dispersion formed. 45 gms of petrolatum were mixed with 5 gms of Vitamin E acetate and 7.4 gms of Parsol MCX in part 3 and heated to 60° C. while mixing and continued until a clear mixture was obtained. The mixture obtained in Part 2 was heated to 60° C. The mixtures obtained in Part 2 and Part 3 were combined and cooled to 30° C. 1% Sodium Hyaluronate in water and triethanolamine in part 4 were mixed with the 30° C. mixture and stirred until thickened. Phenonip (0.5 gram) was added as a preservative. 
       Example 13 
       [0045]    This example illustrates the preparation of a formulation with a product prepared in Example 2. 
         [0000]    
       
         
               
               
               
               
             
               
               
               
               
             
           
               
                   
               
               
                   
                   
                 Percent by 
                 Grams 
               
               
                 Part 
                 Ingredient 
                 Weight 
                 required 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 A 
                 Vitamin E 
                 5.0 
                 5.0 
               
               
                   
                 Robane 
                 4.0 
                 4.0 
               
               
                   
                 Silicon Quat 
                 8.0 
                 8.0 
               
               
                   
                 Microemultion 
                   
                   
               
               
                   
                 Parsol MCX 
                 5.0 
                 5.0 
               
               
                   
                 Triethanolamine 
                 0.4 
                 0.4 
               
               
                 B 
                 product in 
                 64.4 
                 64.4 
               
               
                   
                 Example 2 
                   
                   
               
               
                   
                 Carbopol 934 
                 0.4 
                 0.4 
               
               
                 C 
                 Water 
                 10.0 
                 10.0 
               
               
                   
                 Glycerol 
                 2.0 
                 2.0 
               
               
                 D 
                 phenonip 
                 0.5 
                 0.5 
               
               
                   
                 Rose Natural 
                 0.3 
                 0.3 
               
               
                   
                 water soluble 
               
               
                   
               
             
          
         
       
     
         [0046]    In part A 5 gms of Vitamin E, 4 gms of Robane, 8 gms of Quat silicone microemulsion, and 5 gms of Parsol MCX were combined and mixed until a uniform mixture was obtained. 0.4 gm of Triethanolamine was added and mixed thoroughly. The ingredients in Part B were mixed in a separate container, 0.4 gm of Carbopol 934 was dispersed into 64.4 gram of the product obtained in Example 2 and stirred until completely mixed. Water and Glycerol in Part C were mixed together then combined with Part B and thoroughly mixed. The mixture comprising Part B and Part C was Mixed with Part A and stirred until a thick uniform mixture was obtained. Phenonip (0.5 gram) was added as a preservative. Rose natural water soluble was added at the end.