Abstract:
Provided are an anti-inflammatory agent containing, as an active ingredient, at least one selected from amino acid derivatives represented by formula (I)                            
     wherein Ar represents an optionally substituted 2-hydroxyaryl group, n is 0 or 1, R 2  represents a hydrogen atom or a side chain of an α-amino acid or β-amino acid, X represents —O— or —NH—, R 1  represents a hydrogen atom or a group that forms, together with R 2  and an adjacent atoms, a cyclic structure of pyroglutamic acid, and R 3  represents a hydrogen atom, an alkyl group having from 1 to 22 carbon atoms or an alkenyl group having from 2 to 22 carbon atoms, and salts thereof, and toiletries or skin external products containing the same. The anti-inflammatory agent of the invention inhibits expression of an inflammatory protein and activation of a gene transcription control factor that participates therein, and exhibits a good feeling upon use and a safety.

Description:
BACKGROUND OF THE INVENTION 
     1. Field of the Invention 
     The present invention relates to an anti-inflammatory agents that is useful for preventing, improving or treating inflammatory skin injuries or diseases, and skin external products and toiletries containing the same. 
     2. Description of the Related Art 
     In recent years, the causes of various skin injuries and diseases have been increasingly studied. For example, it is known that with respect to the causes of senescence, canceration, pigmentation and inflammation, inflammatory cytoklines such as IL-1α and TNF-α and extracellular matrix decomposition enzymes such as collagenase deeply participate therein (for example, “Oxidative Stress in Dermatology”, Marcel Dekker, Inc., pp. 187-205, 1993). The expression of genes encoding these proteins is mainly controlled at a gene transcription level. Regarding the inflammatory proteins such as inflammatory cytokines and extracellular matrix decomposition enzymes, the expression thereof is controlled by transcription control factors such as NF-κB and AP-1 (for example, “Active Oxygen and Signal Transfer”, Kodansha Scientific. pp. 37-46, 1996). Accordingly, when the expression of inflammatory proteins or the activation of transcription control factors participating therein can be inhibited, it is expected to prevent skin injuries and diseases. 
     For example, it is indicated that sulfur-containing antioxidants such as N-acetyl-L-cysteine and pyrrolidine dithiocarbamate inhibit NF-κB activation (for example, “Active Oxygen and Signal Transfer”, Kodansha Scientific, pp. 37-46, 1996). N-acetyl-L-cysteine is reported to inhibit also AP-1 activation (for example, FEBS Letters, vol. 384, pp. 92-96, 1996). These compounds are however problematic in the feeling upon use owing to a peculiar odor derived from a sulfur atom present in the molecular structure thereof. Besides the sulfur-containing antioxidants, AP-1 activation and expression of extracellular matrix decomposition enzymes by retinoic acid (for example, Nature, vol. 379, pp. 335-339, 1996) and inhibition of NF-κB activation by a steroidal anti-inflammatory drug or a non-steroidal anti-inflammatory drug (for example, Bio Essays, vol. 18, pp. 371-378, 1996) have been reported. Nevertheless, retinoic acid has a side effect such as skin peeling, and a steroidal anti-inflammatory drug has a side effect such as steroidal dermatosis. Accordingly, the use thereof is limited. Although a non-steroidal anti-inflammatory drug is free from a systemic side effect encountered in the steroidal anti-inflammatory drug, a local side effect thereof has to be improved, and further, an effect of inhibiting inflammatory factor activation is unsatisfactory. 
     SUMMARY OF THE INVENTION 
     It is an object of the invention to provide an anti-inflammatory agents which inhibits expression of an Inflammatory protein and activation of a gene transcription control factor participating therein and which exhibits a good feeling upon use and a safety. 
     The present inventors have assiduously conducted investigations to achieve the object, and have consequently found that the object is achieved by using amino acid derivatives represented by the following formula (I) or salts thereof as an active ingredient. This finding has led to the completion of the invention. 
     That is, the invention relates to an anti-inflammatory agents containing, as an active ingredient, at least one selected from amino acid derivatives represented by formula (I)                           
     wherein 
     Ar represents an optionally substituted 2-hydroxyaryl group, 
     n is 0 or 1. 
     R 2  represents a hydrogen atom or a side chain of an α-amino acid or a β-amino acid, 
     X represents —O— or —NH—, 
     R 1  represents a hydrogen atom or a group that forms, together with R 2  and an adjacent atoms, a cyclic structure of pyroglutamic acid, and 
     R 3 represents a hydrogen atom, an alkyl group having from 1 to 22 carbon atoms or an alkenyl group having from 2 to 22 carbon atoms, 
     and salts thereof. 
     Further, the invention relates to an agent for preventing or treating inflammatory diseases, especially ultraviolet induction inflammatory diseases which agent contains at least one of the amino acid derivatives represented by formula (I) and the salts thereof. 
     Still further, the invention relates to a toiletry additive which is added as a toiletry component, this toiletry additive being composed of at least one of the amino acid derivatives represented by formula (I) and the salts thereof. 
     Furthermore, the invention relates to toiletries or skin external products containing at least one selected from the amino acid derivatives represented by formula (I) and the salts thereof. The toiletries of the invention are useful for preventing or improving inflammatory skin injuries, and the skin external products of the invention are useful for preventing or treating inflammatory diseases. 
     Of the compounds represented by formula (I), the compounds represented by formula (II) are novel compounds undescribed in the literature or publication.                           
     wherein 
     Ar may be substituted with 2-hydroxyaryl group, 
     m is 0 or 1, 
     R 5  represents a side chain selected from the group of alanine, phenylalanine, serine, cysteine. aspartic acid, cysteic acid, homocysteic acid, ornithine or histidine when m is 0 and , R 5  represents hydrogen atom when m is 1, 
     R 4  represents a hydrogen atom or a group that forms, together with R 5  and adjacent atoms, a cyclic structure of pyroglutamic acid, and 
     Y represents —OR 6 , —NHR 6  or —NH 2 , and 
     R 6  represents alkyl group having from 1 to 7 carbon atoms. 
     DETAILED DESCRIPTION OF THE INVENTION 
     The present invention will be explained in detail as follows. 
     In the compounds represented by formula (I), n is an Integer of 0 or 1. When n is 0, the compounds represented by formula (I) are β-amino acid derivatives. When n is 1, the compounds represented by formula (I) are β-amino acid derivatives. 
     When n is 0, R 2  in the amino acid derivatives represented by formula (I) is a hydrogen atom or a side chain of an α-amino acid. Examples of the side chain of the α-amino acid include side chain of acidic amino acids such as glutamic acid, aspartic acid, cysteic acid and homocysteic acid, neutral amino acids such as glycine, alanines valine, leucine, isoleucine, phenylalanine, tryptophan, threonine, serine, homoserine,tyrosine, dopa, cysteine, methionine, glutamine and asparagine, and basic amino acids such as lysine, ornithine, arginine and histidine. Side chains of neutral amino acids are preferable. 
     Further, when n is 1, R 2  may be a hydrogen atom or a side chain of a β-amino acid. As the β-amino acid, β-alanine is preferable. 
     In the amino acid derivatives represented by formula (I), R 1  is usually a hydrogen atom. However, R 1  can also form, together with R 2  and an adjacent atom, a cyclic structure: As the cyclic structure, a 2-pyrrolidone ring is preferable. 
     In this case, the compounds represented by formula (I) are pyroglutamic acid derivatives. 
     When an asymmetric carbon atom is present in the amino acid residue, the compounds may be either optically active compounds or racemic compounds. 
     The alkyl group in R 3  of formula (I) is a linear or branched alkyl group having from 1 to 22 carbon atoms, preferably from 1 to 18 carbon atoms, and it may have an unsaturated group in a part of a carbon chain. Examples of the alkyl group in the invention include methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-amyl, sec-amyl, tert-amyl, isoamyl, n-hexyl, cyclohexyl, n-heptyl, n-octyl, 2-ethylhexyl, nonyl, isononyl, decyl, isodecyl, undecyl, lauryl, tridecyl, isotridecyl, myristyl, cetyl, isocetyl, stearyl, isostearyl, behenyl groups and so on. 
     Further, the alkenyl group is a linear or branched alkenyl group having from 2 to 22 carbon atoms, preferably from 2 to 18 carbon atoms, more preferably from 5 to 18 carbon atoms which group may have at least one of carbon-carbon double bonds or carbon-carbon triple bonds as required. An alkenyl group derived from unsaturated fatty acids, such as an oleyl group, is preferable. 
     The 2-hydroxyaryl group in Ar group in formulae (I) and (II) is not particularly limited so long as it is an aromatic group having a hydroxyl group in the 2-position of a 5- or 6-membered aromatic ring. This aryl group is a monocyclic, polycyclic or fused-ring aromatic hydrocarbon group having at least one 6 -membered aromatic ring, or a monocyclic, polycyclic or fused-ring aromatic heterocyclic group having a 5- to 8-membered heterocyclic ring containing at least one of hetero-atoms such as nitrogen, oxygen and sulfur atoms, these groups having from 6 to 21 carbon atoms, preferably from 6 to 14 carbon atoms, more preferably from 6 to 12 carbon atoms. 
     These 2-hydroxyaryl groups may optionally be substituted unless it has an adverse effect on the activity of inhibiting inflammatory factor activation in the invention. At this time, examples of the substituent include the above-mentioned alkyl groups, alkoxy groups corresponding to the alkyl groups, alkoxycarbonyl groups corresponding to the alkyl groups, halogen atoms such as chlorine, fluorine and bromine, a hydroxyl group, a carboxyl group, nitro group and so on. 
     Specific examples of the optionally substituted 2-hydroxyaryl group of formulae (I) and (II) include 2-hydroxyphenyl, 2-hydroxy-1-naphthyl, 1-hydroxy-2-naphthyl, 2-hydroxy-4-methoxyphenyl, 2-hydroxy-3-methoxyphenyl, 5-bromo-2-hydroxyphenyl, 5-chloro-2-hydroxyphenyl, 2-hydroxy-5-nitrophenyl, 3,5-dibromo-2-hydroxyphenyl, 3,5-dichloro-2-hydroxyphenyl, 2,3-dihydroxyphenyl, 2,4-dihydroxyphenyl, 2,5-dihydroxyphenyl groups and so on. 
     In the compounds represented by formula (II), m is an integer of 0 or 1. When m is 0, the compounds represented by formula (II) are α-amino acid derivatives. When m is 1, the compounds represented by formula II) are β-amino acid derivatives. 
     When the compounds represented by formula (II) are α-amino acid derivatives (that is, when m is 0), examples of the amino acid include alanine, phenylalanine, serine, cysteine, aspartic acid, cysteic acid, homocysteic acid, ornithine and histidine. In this case, the substituent R 5  represents the side chain of the amino acid. When the compounds represented by formula (II) are β-amino acid derivatives (that is, m is 1), the amino acid is β-alanine.In this case, the substituent R 5  represents a hydrogen atom. 
     In the compounds represented by formula (II), the substituent R 4 is usually a hydrogen atom. However, R 4  can also form, together with R 6  and an adjacent atoms, a cyclic structure. 
     As the cyclic structure, a 2-pyrrolidone ring is preferable. 
     In this case, the compounds represented by formula (II) are pyroglutamic acid derivatives. 
     In the compounds represented by formula (II), Y is —OR 6 , —NHR 6  or —NH 2  in which R 6  represents an alkyl group having from 1 to 7 carbon atoms. The alkyl group having from 1 to 7 carbon atoms in R 6  is a linear or branched saturated alkyl group. Examples of the alkyl group include methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-amyi, sec-amyl, tert-amyl, isoamyl and n-hexyl groups. 
     Accordingly, —OR 6  in the substituent Y is an alkoxy group having from 1 to 7 carbon atoms. Examples of the alkoxy group include methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy, tert-butoxy, n-amyloxy, sec-amyloxy, tert-amyloxy, isoamyloxy and n-hexyloxy groups. 
     Further, —NHR 6  in the substituent Y is an N-alkylamino group, and it is an amino group substituted by the above-mentioned alkyl group. 
     Examples of the salts of the compounds represented by formulae (I) and (II) include inorganic acid salts such as a hydrochloride, a sulfate, a carbonate and a phosphate; and organic acid salts such as an acetate, a tartrate, a citrate, a p-toluenesulfonate, a glycolate, a malate, a lactate, a fatty acid salt, an acidic amino acid salt, pyroglutamate and so on. These salts may be used either singly or in combination. They may be incorporated as amino acid derivative salts, or amino acid derivative salts may be formed in a composition by incorporating amino acid derivatives and salts separately. 
     The amino acid derivatives represented by formula (I) can easily be formed by reacting a 2-hydroxy-aromatic aldehyde such as salicylaldehyde with an amino acid alkyl ester or an amino acid alkylamide in the presence or absence of a solvent and adding a hydrogenation agent such as sodium boron hydride to the reaction mixture. 
     Or it can also be formed by reacting a 2-hydroxy-aromatic aldehyde with an amino acid to form a Schiff base, adding thereto a hydrogenation agent such as sodium boron hydride to obtain an N-(2-hydroxy-aromatic-1-methylene) amino acid, and then esterifying or amidating the same. 
     Examples of the 2-hydroxy-aromatic aldehyde include, other than salicylaldehyde, 2-hydroxy-1-naphtoaldehyde, pyridoxal, 2-hydroxy-4-methoxybenzaldehyde, o-vanillin, 5-bromosalicylaldehyde, 5-chlorosalicylaldehyde, 5-nitrosalicylaldehyde, 3,5-dibromosalicylaldehyde, 3,5-dichlorosalicylaldehyde, 2,3-dihydroxybenzaldehyde, 2,4-dihydroxybenzaldehyde, 2,5-dihydroxybenzaldehyde and so on. 
     The anti-inflammatory agents of the invention can be administered either orally or parenterally. It is preferable to administer the same directly to an inflammatory factor activation system. Usually, it is preferable that the inhibitor is used by being incorporated into toiletries or skin external products. For examples when the inhibitor is incorporated into toiletries as an active ingredient to prevent or improve inflammatory skin injuries, it may be added in an amount of from 0.01 to 10% by weight, preferably from 0.1 to 5% by weight. Further, when the inhibitor is incorporated into skin external products as an active ingredient for preventing or treating inflammatory diseases, it may be added in an amount of from 0.01 to 50% by weight, preferably from 0.1 to 20% by weight. When the amount is less than 0.01% by weight, the activity of inhibiting inflammatory factor activation is not satisfactorily exhibited, and it is thus undesirable When the amount exceeds 50% by weight, the feeling upon use is problematic in that a dry and hard feeling is given to the skin. Thus, it is undesirable. 
     When the anti-inflammatory agent of the invention is incorporated into toiletries or skin external products, components that are generally used in toiletries or skin external products can be added, other than the anti-inflammatory agent of the invention, unless the effects of the invention are impaired. Examples of the components which are generally used in toiletries or skin external products include an oily material, a surfactant, a solvent, a wetting agent, a high-molecular substance, a powder product, a dyestuff, a flavor and so on. 
     Examples of the oily material include oils such as animal and vegetable oils, waxes such as lanolin, hydrocarbons such as paraffin, higher alcohols such as cetanol, higher fatty acids such as stearic acid, sterols, phospholipids such as lecithin, synthetic esters such as myristic acid, metallic soaps, a silicone oil and so on. 
     Examples of the surfactant include an anionic surfactant, a cationic surfactant, an ampholytic surfactant, a nonionic surfactant, an emulsifying agent, a solubilizing agent and so on. 
     Examples of the solvent include lower alcohols such as ethanol, ethers, glycerols, liquid nonionic surfactants, liquid oily materials, other organic solvents and water. 
     Examples of the wetting agent include polyhydric alcohols such as glycerol, salts of organic acids such as pyrrolidonecarboxylic acid, urea, mucopolysaccharides such as hyaluronic acid, and salts of amino acids such as proline. 
     Examples of the high-molecular substance include natural high-molecular compounds such as collagen. semisynthetic high-molecular compounds such as a partially deacetylated chitin, and synthetic high-molecular compounds such as carboxymethyl cellulose. 
     Examples of the powdery product include inorganic pigments such as talc, functional pigments such as synthetic mica, hybrid fine powders, pearlessence pigments such as titanium dioxide-coated mica, photochromic pigments, high-molecular powders such as a nylon powder, and organic powders such as Nε-lauroyl lysine. 
     Examples of the dyestuff include a legal tar dyestuff first group, a legal tar dyestuff second group, a legal tar dyestuff third group, a hairdye, a natural dyestuff and a mineral dyestuff. 
     Examples of the flavor include animal flavors such as musk, vegetable flavors such as a jasmine oil, synthetic flavors such as α-amylcinnamaldehyde, and mixed flavors. 
     The form of the toiletries or the skin external products containing the anti-inflammatory agent of the invention is not particularly limited. The toiletries or the skin external products may take the form of a solution, a paste, a gel, a solid or a powder. They may be used in an oil, a lotion, a cream, a milky lotion, a gel, a shampoo, a hair rinse, a hair conditioner, an enamel, a foundation, a lipstick, a cosmetic powder, a pack, an ointment, a tablet, an injection, a granule, a capsule, a perfume, a powder, an eau de Cologne, a toothpaste, a soap, an aerosol and a cleansing foam, as well as in an agent for preventing or improving skin aging, an agent for preventing or improving skin inflammation, a bath product, a hair tonic, a skin beauty lotion, an anti-sunburn agent, an agent for preventing or improving photodermatosis such as xeroderma pigmentosum or solar urticaria, an agent for preventing or improving photoallergy, an agent for preventing or improving photo-immunosuppression and an agent for preventing or improving skin irritation by injuries, chaps or cracks. Further, they can be used as an agent for preventing or treating various diseases caused by inflammatory factor activation, for example, rheumatoid diseases such as chronic rheumatism, arthritis, cutaneous diseases such as atopic dermatitis, contact dermatitis and psoriasis vulgaris, respiratory diseases such as bronchial asthma and bronchitis, inflammatory bowel diseases, acute or chronic hepatitis, acute or chronic nephritis, Mediterranean fever, and ischemic diseases such as myocardial infarction. 
     Moreover, other ordinary components in toiletries or skin external products can be added to the toiletries or the skin external products containing the anti-inflammatory agent of the invention unless the effects of the invention are impaired. The other ordinary components in toiletries or skin external products include an antiseptic, a disinfectant, an antioxidant, an ultraviolet absorber, a chelating agent, a discoloration preventing agent, a buffer, a drug for an acne, an agent for preventing dandruff and itching, an antiperspirant, a burn agent, an acaricidal and louse-killing agent, a keratin softening agent, a xerosis agent, an antiviral agent, a percutaneous absorption accelerator, hormones, vitamins, amino acids, peptides, proteins, an astringent, an anti-inflammatory agent, a refrigerant, a stimulant, components derived from animals and vegetables, a melanin synthesis inhibitor (whitening agent), antibiotics, an antifungal agent and a hair tonic. 
     The anti-inflammatory agent of the invention has an excellent activity of inhibiting inflammatory factor activation. Further, when the toiletries or the skin external products containing the anti-inflammatory agent of the invention are coated on the skin, these effectively remain on the skin, are hard to drop and have an excellent feeling upon use. 
    
    
     EXAMPLES 
     The invention is illustrated more specifically by referring to the following Examples. However, the invention is not limited thereto. In Examples, the amount was expressed in terms of % by weight. 
     Synthesis Example 1 
     Nine grams of glycine were dissolved in 60 ml of a 2N sodium hydroxide aqueous solution. To the solution were then added 12 ml of salicylaldehyde and 1.3 g of sodium boron hydride in this order. After the mixture was stirred for 1 hour, 12 ml of salicylaldehyde and 1.3 g of sodium boron hydride were added thereto again. After the resulting mixture was stirred at room temperature for 1 hour, the insoluble matter was separated through filtration, and the filtrate was extracted with diethyl ether. The extract was adjusted to a pH of 4 with hydrochloric acid to obtain 17 g of N-(2 -hydroxybenzyl) glycine. Various N-(2-hydroxybenzyl) amino acids were obtained in the same manner. 
     Synthesis Example 2 
     L-serine (3.6 g) was dissolved in 17 ml of a 2N sodium hydroxide aqueous solution. To the solution were then added 3.6 ml of salicylaldehyde and 0.4 g of sodium boron hydride in this order. After the mixture was stirred for 1 hour, 3.6 ml of salicylaldehyde and 0.4 g of sodium boron hydride were added thereto again. After the resulting mixture was stirred overnight at room temperature, the insoluble matter was separated through filtration, and the filtrate was extracted with diethyl ether. The extract was adjusted to a pH of 7 with hydrochloric acid to obtain 4.8 g of N-(2-hydroxybenzyl)-L-serine. To 300 mg of the resulting N-(2-hydroxybenzyl)-L-serine were added 30 ml of ethanol to which a hydrogen chloride gas had been blown to saturation. The mixture was stirred overnight. The reaction solution was concentrated, and the resulting oil was purified through HPLC using Inertcil ODS-3 column (supplied by GL Science) in a high performance liquid chromatography (supplied by Hitachi) to obtain 114 mg of N-(2-hydroxybenzyl)-L-serine ethyl ester. Various N-(2-hydroxybenzyl) amino acid alkyl esters were obtained in the same manner. With respect to the novel compounds undescribed in the literature among these compounds, the results of the measurement of the mass spectrum are shown in Table 1. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                   
                 TABLE 1 
               
             
             
               
                   
                   
               
               
                   
                 mass 
                   
               
               
                   
                 spectral 
               
               
                   
                 (M + H + ) 
               
             
          
           
               
                 Novel Compound Not-Described in Publication 
                 A 
                 B 
               
               
                   
               
               
                 N-(2-hydroxybenzyl)-L-alanine ethyl ester 
                 224 
                 224 
               
               
                 N-(2-hydroxybenzyl)-D-alanine ethyl ester 
                 224 
                 224 
               
               
                 N-(2-hydroxybenzyl)-L-serine ethyl ester 
                 240 
                 240 
               
               
                 N-(2-hydroxybenzyl)-L-histidine ethyl ester 
                 290 
                 290 
               
               
                 N-(2-hydroxybenzyl)-L-pyroglutamic acid ethyl ester 
                 264 
                 264 
               
               
                 N-(2-hydroxybenzyl)-L-phenylalanine ethyl ester 
                 300 
                 300 
               
               
                 N-(2-hydroxybenzyl)-L-alanine isopropyl ethyl ester 
                 238 
                 238 
               
               
                   
               
               
                 A: measured  
               
               
                 B: calculated  
               
             
          
         
       
     
     Synthesis Example 3 
     L-alanine (2.9 g) was dissolved in 20 ml of a 2N sodium hydroxide aqueous solution. To the solution were then added 3.5 ml of salicylaldehyde and 0.4 g of sodium boron hydride in this order. After the mixture was stirred for 1 hour, 3.5 ml of salicylaldehyde and 0.4 g of sodium boron hydride were added thereto again. After the resulting mixture was stirred at room temperature for 1 hour, the insoluble matter was separated by filtration, and the filtrate was extracted with diethyl ether. The extract was adjusted to a pH of 6 with hydrochloric acid to obtain 5.8 g of N-(2-hydroxybenzyl)-L-alanine. The resulting N-(2-hydroxybenzyl) -L-alantne ( 4.6 g) and 8.8 g of 1-dodecanol were added to 150 ml of toluene. A hydrogen chloride gas was then added thereto to saturation. Ten grams of molecular sieves were added thereto, and the mixture was stirred overnight. The insoluble matter was separated by filtration, and the filtrate was then concentrated. The resulting oil was dissolved in methylene chloride, and washed with a saturated aqueous solution of sodium chloride. The product was dried over magnesium sulfate, and then concentrated under reduced pressure to obtain 8g of N-(2-hydroxybenzyl)-L-alanine lauryl ester. 
     Various N-(2-hydroxybenzyl) amino acid alkyl esters were obtained in them same manner. 
     Synthesis Example 4 
     L-alanine laurylamide (25 g) and 1 g of sodium hydroxide were dissolved in 20 ml of methanol. To the solution were added 1.0 ml of salicylaldehyde and 0.1 g of sodium boron hydride in this order. After the mixture was stirred for 1 hour, 1.0 ml of salicylaldehyde and 0.1 g of sodium boron hydride were added thereto again in this order. After the resulting mixture was stirred overnight at room temperature, the insoluble matter was separated through filtration, and the residue was adjusted to a pH of 7 with hydrochloric acid. The oil was obtained through concentration under reduced pressure was dissolved in diethyl ether, washed with water, and then dried over magnesium sulfate. After the drying agent was separated through filtration, the filtrate was concentrated under reduced pressure to obtain 3 g of N-(2-hydroxybenzyl)-L-alanine laurylamide. 
     Various N-(2-hydroxybenzyl)amino acid alkyl amides were obtained in the same manner. With respect to the novel compounds undescribed in the literature among these compounds, the results of the measurement of the mass spectrum are shown in Table 2. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                   
                 TABLE 2 
               
             
             
               
                   
                   
               
               
                   
                 mass 
                   
               
               
                   
                 spectral 
               
               
                   
                 (M + H + ) 
               
             
          
           
               
                 Novel Compound Not-Described in Publication 
                 A 
                 B 
               
               
                   
               
               
                 N-(2-hydroxybenzyl)-L-alanine ethyl ester 
                 223 
                 223 
               
               
                 N-(2-hydroxybenzyl)-L-phenyl alanine ethyl amide 
                 299 
                 299 
               
               
                   
               
               
                 A: measured  
               
               
                 B: calculated  
               
             
          
         
       
     
     Test Example 1 
     Test for an Activity of Inhibiting NF-κB Activation 
     The test compound was added to human epidermal cells which had become confluent in a culture plate. Eighteen hours later, the culture solution was replaced with a phenol red-free medium. The cells were subjected to UV irradiation (UVB: 50 mJ/m 2 )using Dermaray M-DMR-80 (supplied by Toshiba Iryo Yohin KK). After from 4 to 5 hours passed, the cells were recovered, and the nucleoproteins were extracted in a usual manner. With respect to the resulting nucleoproteins, NF-κB activated by gel shift assay was detected. The amount of NF-κB was determined by measuring a radioactivity of an NF-κB band using a bio-imaging analyzer BAS 2000 (supplied by Fuji Photo Film). The rate of inhibition of NF-κB activation on the test compound was calculated using formula (III). 
     
       
         Rate (%) of inhibition of NF-κB activation={1−( A   1   −A   3 )/( A   2   −A   3 )}×100  (III) 
       
     
     wherein 
     A 1 : radioactivity of an NF-κB band in the addition of the test compound 
     A 2 : radioactivity of an NF-κB band in the non-addition of the test compound 
     A 3 : radioactivity of an NF-κB band when the test compound was not added, nor was UV irradiation conducted 
     The results are shown in Table 3. 
     
       
         
               
               
               
             
           
               
                 TABLE 3-1 
               
               
                   
               
               
                 Test Compound 
                 A 
                 B 
               
               
                   
               
             
             
               
                 N-(2-hydroxybenzyl)glycine 
                   5 mM 
                 36 
               
               
                   
                  10 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-alanine 
                   1 mM 
                 20 
               
               
                   
                  10 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-serine 
                  10 mM 
                 17 
               
               
                   
                  30 
                 34 
               
               
                 N-(2-hydroxybenzyl)-L-alanine ethyl ester 
                 0.2 mM 
                 68 
               
               
                   
                   2 
                 77 
               
               
                 N-(2-hydroxybenzyl)-D-alanine ethyl ester 
                   1 mM 
                 90 
               
               
                   
                   2 
                 87 
               
               
                 N-(2-hydroxybenzyl)-L-leucine ethyl ester 
                   1 mM 
                 84 
               
               
                   
                   2 
                 57 
               
               
                 N-(2-hydroxybenzyl)-L-histidine ethyl ester 
                   1 mM 
                 69 
               
               
                   
                   2 
                 72 
               
               
                 N-(2-hydroxybenzyl)-L-serine ethyl ester 
                   1 mM 
                 37 
               
               
                   
                   2 
                 56 
               
               
                 N-(2-hydroxybenzyl)-L-pyroglutamic acid ethyl ester 
                   1 mM 
                 53 
               
               
                   
                   2 
                 85 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosine ethyl ester 
                 0.1 mM 
                 64 
               
               
                   
                 0.5 
                 81 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     
       
         
               
               
               
             
           
               
                 TABLE 3-2 
               
               
                   
               
               
                 Test Compound 
                 A 
                 B 
               
               
                   
               
             
             
               
                 N-(2-hydroxybenzyl)-L-alanine lauryl ester 
                  10 μM 
                 20 
               
               
                   
                 100 
                 81 
               
               
                 N-(2-hydroxybenzyl)-D-alanine lauryl ester 
                  50 μM 
                 60 
               
               
                   
                 100 
                 59 
               
               
                 N-(2-hydroxybenzyl)-L-leucine lauryl ester 
                  10 μM 
                 21 
               
               
                   
                  20 
                 23 
               
               
                 N-(2-hydroxybenzyl)-L-serine lauryl ester 
                  10 μM 
                 — 
               
               
                   
                  50 
                 79 
               
               
                 N-(2-hydroxybenzyl)-L-glutamic acid lauryl ester 
                  10 μM 
                 — 
               
               
                   
                 100 
                 45 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosine lauryl ester 
                  5 μM 
                 20 
               
               
                   
                  10 
                 43 
               
               
                 N-(2-hydroxybenzyl)-L-alanine stearyl ester 
                  10 μM 
                 31 
               
               
                   
                 100 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-alanine ethyl amide 
                  1 mM 
                 43 
               
               
                   
                  2 
                 26 
               
               
                 N-(2-hydroxybenzyl)-L-phenylalanine ethyl amide 
                  0.5 mM 
                 102 
               
               
                   
                  1 
                 47 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosine ethyl amide 
                  1 mM 
                 72 
               
               
                   
                  2 
                 28 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 3-3 
               
               
                   
               
               
                 Test Compound 
                 A 
                 B 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 N-(2-hydroxybenzyl)-L-alanine lauryl amide 
                  10 μM 
                 53 
               
               
                   
                  20 
                 65 
               
               
                 N-(2-hydroxybenzyl)-D-alanine lauryl amide 
                  10 μM 
                 29 
               
               
                   
                  50 
                 97 
               
               
                 N-(2-hydroxybenzyl)-L-leucine lauryl amide 
                  10 μM 
                 40 
               
               
                   
                  50 
                 89 
               
               
                 N-(2-hydroxybenzyl)-L-pyroglutamic acid lauryl amide 
                  50 μM 
                 75 
               
               
                   
                 100 
                 74 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosine lauryl amide 
                  10 μM 
                 39 
               
               
                   
                 100 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-arginine lauryl amide 
                  10 μM 
                 16 
               
               
                   
                  50 
                 28 
               
               
                 N-acetyl-L-cysteine 
                  10 mM 
                 23 
               
               
                   
                  30 
                 55 
               
               
                 pyrrolidinedithiocarbamate 
                  5 μM 
                 −9 
               
               
                   
                  10 
                 9 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     As shown in Table 3, the products all exhibit the activity of inhibiting NF-κB activation at lower concentrations than pyrrolidine dithiocarbamate, a known NF-κB activation inhibitor, and have a high activity of inhibiting inflammatory factor activation. 
     Test Example 2 
     Test for an Activity of Inhibiting AP-1 Activation 
     The test compound was added to human dermal fibroblasts which had become confluent in a culture plate. Eighteen hours later, the culture solution was replaced with a phenol red-free medium. The cells were subjected to UV irradiation (UVA: 20 J/cm 2 ) using Dermaray M-DMR-80 (supplied by Toshiba Iryo Yohin KK). After from 4 to 5 hours passed, the cells were recovered, and the nucleoproteins were extracted in a usual manner. With respect to the resulting nucleoproteins, AP-1 activated by gel shift assay was detected. The amount of AP-1 was determined by measuring a radioactivity of an AP-1 band using a bio-imaging analyzer BAS 2000 (supplied by Fuji Photo Film). The rate of inhibition of AP-1 activation on the test compound was calculated using formula (IV). 
     
       
         Rate (%) of inhibition of AP-1 activation={1−( A   4   −A   6 )/( A   5   −A   6 )}×100  (IV) 
       
     
     wherein 
     A 4 : radioactivity of an AP-1 band in the addition of the test compound 
     A 5 : radioactivity of an AP-1 band in the non-addition of the test compound 
     A6: radioactivity of an AP-1 band when the test compound was not added, nor was UV irradiation conducted 
     The results are shown in Table 4. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 4-1 
               
               
                   
               
               
                 Test Compound 
                 A 
                 B 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 N-(2-hydroxybenzyl)-L-alanin ethyl ester 
                 0.5 mM 
                 26 
               
               
                   
                   2 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-histidine ethyl ester 
                 0.5 mM 
                 20 
               
               
                   
                   1 
                 38 
               
               
                 N-(2-hydroxybenzyl)-L-serine ethyl ester 
                 0.5 mM 
                 — 
               
               
                   
                   1 
                 73 
               
               
                 N-(2-hydroxybenzyl)-L-alanine isopropyl ester 
                 0.1 mM 
                 20 
               
               
                   
                 0.5 
                 35 
               
               
                 N-(2-hydroxybenzyl)-L-alanine lauryl ester 
                  10 mM 
                 68 
               
               
                   
                  50 
                 66 
               
               
                 N-(2-hydroxybenzyl)-L-serine lauryl ester 
                   1 μM 
                 43 
               
               
                   
                  10 
                 49 
               
               
                 N-(2-hydroxybenzyl)-L-glutamic acid lauryl ester 
                   1 μM 
                 — 
               
               
                   
                  10 
                 33 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosine lauryl ester 
                   1 μM 
                 19 
               
               
                   
                  10 
                 45 
               
               
                 N-(2-hydroxybenzyl)-L-alanine stearyl ester 
                   1 μM 
                 20 
               
               
                   
                   5 
                 48 
               
               
                 N-(2-hydroxybenzyl)-L-alanine ethyl amide 
                   1 mM 
                 52 
               
               
                   
                   2 
                 131 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 4-2 
               
               
                   
               
               
                 Test Compound 
                 A 
                 B 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 N-(2-hydroxybenzyl)-L-phenylalanine ethyl amide 
                  0.5 mM 
                 34 
               
               
                   
                  1 
                 61 
               
               
                 N-(2-hydroxybenzyl)-L-alanine lauryl amide 
                  10 μM 
                 57 
               
               
                   
                  20 
                 56 
               
               
                 N-(2-hydroxybenzyl)-D-alanine lauryl amide 
                  1 μM 
                 14 
               
               
                   
                  20 
                 31 
               
               
                 N-(2-hydroxybenzyl)-L-pyroglutamic acid lauryl amide 
                  10 μM 
                 — 
               
               
                   
                 100 
                 42 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosine lauryl amide 
                  10 μM 
                 40 
               
               
                   
                  20 
                 55 
               
               
                 N-(2-hydroxybenzyl)-L-alanine stearyl amide 
                  1 μM 
                 72 
               
               
                   
                  5 
                 47 
               
               
                 dexamethasone 
                  1 μM 
                 39 
               
               
                   
                  10 
                 34 
               
               
                 aspirin 
                  1 mM 
                 −4 
               
               
                   
                  5 
                 14 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     As shown in Table 4, the products all exhibit the activity of inhibiting AP-1 activation which exceeds that of aspirin, a non-steroidal anti-inflammatory drug, and have a high activity of inhibiting inflammatory factor activation. 
     Test Example 3 
     Test for an Activity of Inhibiting IL-1α Expression 
     The test compound was added to human epidermal cells which had become confluent in a culture plate. Eighteen hours later, the culture solution was replaced with a phenol red-free medium. The cells were subjected to UV irradiation (UVB: 50 mJ/cm 2 ) using Dermaray M-DMR-80 (supplied by Toshiba Iryo Yohin KK). After 24 hours passed, the culture solution was recovered, and the IL-1α concentration in the culture solution was measured using IL-1α·ELISA System (supplied by Amersham Corp.). The rate of inhibition of IL-1α expression on the test compound was calculated using formula (V). 
     
       
         Rate (%) of inhibition of IL-1α expression={1−( B   1   −B   3 )/( B   2   −B   3 )}×100  (V) 
       
     
     wherein 
     B 1 : IL-1α concentration in the addition of the test compound 
     B 2 : IL-1α concentration in the non-addition of the test compound 
     B 3 : IL-1α concentration when the test compound was not added, nor was UV irradiation conducted 
     The results are shown in Table 5. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 5-1 
               
               
                   
               
               
                 Test Componnd 
                 A 
                 B 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 N-(2-hydroxybenzyl)glycine 
                   5 mM 
                 26 
               
               
                   
                  10 
                 88 
               
               
                 N-(2-hydroxybenzyl)-L-alanine 
                   1 μM 
                 14 
               
               
                   
                  10 
                 100 
               
               
                 N-(2-hydroxybenzyl)-L-serine 
                  10 mM 
                 62 
               
               
                   
                  30 
                 70 
               
               
                 N-(2-hydroxybenzyl)-L-alanin ethyl ester 
                 0.2 mM 
                 61 
               
               
                   
                   2 
                 100 
               
               
                 N-(2-hydroxybenzyl)-L-serine ethyl ester 
                 0.1 mM 
                 — 
               
               
                   
                   2 
                 44 
               
               
                 N-(2-hydroxybenzyl)-L-pyroglutamic acid ethyl ester 
                 0.5 mM 
                 84 
               
               
                   
                   1 
                 55 
               
               
                 N-(2-hydroxybenzyl)-L-phenylalanine ethyl ester 
                 0.1 mM 
                 25 
               
               
                   
                   1 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-arginine ethyl ester 
                 0.5 mM 
                 65 
               
               
                   
                   1 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-alanine isopropyl ester 
                 0.1 mM 
                 36 
               
               
                   
                   1 
                 53 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     
       
         
               
               
               
             
           
               
                 TABLE 5-2 
               
               
                   
               
               
                 Test Componnd 
                 A 
                 B 
               
               
                   
               
             
             
               
                 N-(2-hydroxybenzyl)-L-alanine lauryl ester 
                  10 μM 
                 23 
               
               
                   
                 100 
                 72 
               
               
                 N-(2-hydroxybenzyl)-D-alanine lauryl ester 
                  10 μM 
                 35 
               
               
                   
                  50 
                 31 
               
               
                 N-(2-hydroxybenzyl)-L-serine lauryl ester 
                  10 μM 
                 12 
               
               
                   
                 100 
                 31 
               
               
                 N-(2-hydroxybenzyl)-L-glutamic acid lauryl ester 
                  10 μM 
                 54 
               
               
                   
                 100 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-alanine stearyl ester 
                  1 μM 
                 25 
               
               
                   
                  10 
                 25 
               
               
                 N-(2-hydroxybenzyl)-L-alanine ethyl amide 
                  0.5 mM 
                 81 
               
               
                   
                  1 
                 99 
               
               
                 N-(2-hydroxybenzyl)glycine lauryl amide 
                  20 μM 
                 13 
               
               
                   
                  50 
                 45 
               
               
                 N-(2-hydrorybenzyl)-L-alanine lauryl amide 
                  10 μM 
                 69 
               
               
                   
                  50 
                 73 
               
               
                 N-(2-hydroxybenzyl)-D-alanine lauryl ainide 
                  20 μM 
                 25 
               
               
                   
                  50 
                 79 
               
               
                 N-(2-hydroxybenzyl)-L-leucine lauryl amide 
                  20 μM 
                 27 
               
               
                   
                  50 
                 79 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 5-3 
               
               
                   
               
               
                 Test Compound 
                 A 
                 B 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 N-(2-hydroxybenzyl)-L-pyroglutamic acid lauryl amide 
                  10 μM 
                 25 
               
               
                   
                  50 
                 28 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosiue lauryl amide 
                  1 μM 
                 — 
               
               
                   
                  10 
                 56 
               
               
                 N-(2-hydroxybenzyl)-L-alanine stearyl auiide 
                  5 μM 
                 30 
               
               
                   
                  10 
                 77 
               
               
                 dexamethasone 
                  10 μM 
                 48 
               
               
                   
                 100 
                 65 
               
               
                 pyrrolidinedithiocarbamate 
                  5 μM 
                 −45 
               
               
                   
                  10 
                 — 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     As shown in Table 5, the products all exhibit the activity of inhibiting IL-1α expression which is equal to or higher than that of dexamethasone, a steroidal anti-inflammatory drug, and have a high activity of inhibiting inflammatory factor activation. 
     Test Example 4 
     Test for an Activity of Inhibiting Collagenase Expression 
     The test compound was added to human dermal fibroblasts which had become confluent in a culture plate. Eighteen hours later, the culture solution was replaced with a phenol red-free medium. The cells were subjected to UV irradiation (UVA; 20 J/cm 2 ) using Dermaray M-DMR-80 (supplied by Toshiba Iryo Yohin KK). After 24 hours passed, the culture solution was recovered, and the collagenase concentration in the culture solution was measured using MMP-1α·ELISA System (supplied by Amersham Corp.). The rate of inhibition of collagenase expression on the test compound was calculated using formula (VI). 
     
       
         Rate (%) of inhibition of collagenase expression={1−( B   4   −B   6 )/( B   5   −B   6 )}×100  (VI) 
       
     
     wherein 
     B 4 : collagenase concentration in the addition of the test compound 
     B 5 : collagenase concentration in the non-addition of the test compound 
     B 6 : collagenase concentration when the test compound was not added, nor was UV irradiation conducted 
     The results are shown in Table 6. 
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 6-1 
               
               
                   
               
               
                 Test Compound 
                 A 
                 B 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 N-(2-hydroxybenzyl)glycine ethyl ester 
                  10 μM 
                 39 
               
               
                   
                 100 
                 46 
               
               
                 N-(2-hydroxybenzyl)-D-alanin ethyl ester 
                  0.1 mM 
                 — 
               
               
                   
                  0.5 
                 108 
               
               
                 N-(2-hydroxybenzyl)-L-histidin ethyl ester 
                  0.1 mM 
                 44 
               
               
                   
                  1 
                 51 
               
               
                 N-(2-hydroxybenzyl)-L-serin ethyl ester 
                  0.5 mM 
                 30 
               
               
                   
                  1 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-alanine lauryl ester 
                  10 μM 
                 58 
               
               
                   
                  50 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-leucine lauryl ester 
                  10 μM 
                 21 
               
               
                   
                  50 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-glutamic acid lauryl ester 
                  1 μM 
                 53 
               
               
                   
                  10 
                 49 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosine lauryl ester 
                  10 μM 
                 61 
               
               
                   
                  50 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-arginine lauryl ester 
                  10 μM 
                 52 
               
               
                   
                  50 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-alanine stearyl ester 
                  1 μM 
                 57 
               
               
                   
                  5 
                 — 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 6-2 
               
               
                   
               
               
                 Test Compound 
                 A 
                 B 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 N-(2-hydroxybenzyl)-L-phenylalanine ethyl amide 
                 0.1 mM 
                 32 
               
               
                   
                 0.5 
                 27 
               
               
                 N-(2-hydroxybenzyl)-L-alanine lauryl amide 
                   1 μM 
                 57 
               
               
                   
                  10 
                 — 
               
               
                 N-(2-hydroxybenzyl)-L-leucine lauryl amide 
                   1 μM 
                 37 
               
               
                   
                  10 
                 60 
               
               
                 N-(2-hydroxybenzyl)-L-pyroglutamic acid 
                  10 μM 
                 23 
               
               
                 lauryl amide 
                  50 
                 52 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosine lauryl amide 
                   1 μM 
                 42 
               
               
                   
                  10 
                 84 
               
               
                 N-(2-hydroxybenzyl)-L-alanine stearyl amide 
                   1 μM 
                 72 
               
               
                   
                   5 
                 — 
               
               
                 dexamethasone 
                   1 μM 
                 27 
               
               
                   
                  10 
                 53 
               
               
                 aspirin 
                   1 mM 
                 −197 
               
               
                   
                   5 
                 −436 
               
               
                   
               
               
                 A: concentration  
               
               
                 B: inhibitory rate (%)  
               
             
          
         
       
     
     As shown in Table 6, the products all exhibit the activity of inhibiting collagenase expression which exceeds that of aspirin, a non-steroidal anti-inflammatory drug, and have a high activity of inhibiting inflammatory factor activation. 
     Anti-inflammatory agents, toiletries and skin external products in Examples 1 to 16 were prepared in a usual manner. 
     
       
         
               
               
             
           
               
                   
               
             
             
               
                 Example 1  Tablet 
                   
               
               
                 N-(2-hydroxybenzyl)-L-alaninelaurylamide 
                   10% by weight 
               
               
                 lactose 
                 50 
               
               
                 starch 
                 20 
               
               
                 carboxymethylcellulose 
                 19 
               
               
                 magnesium stearate 
                 1 
               
               
                 Example 2  Injection 
               
               
                 N-(2-hydroxybenzyl)-L-alanineethylester 
                  0.1% by weight 
               
               
                 glucose 
                 2.0 
               
               
                 injection water 
                 remainder 
               
               
                 Example 3  Ointment 
               
               
                 N-(2-hydroxybenzyl)glycineethylester 
                  1.0% by weight 
               
               
                 urea 
                 20.0 
               
               
                 white vaseline 
                 15.0 
               
               
                 soft liquid paraffin 
                 6.0 
               
               
                 cetanol 
                 3.0 
               
               
                 stearylalcohol 
                 3.0 
               
               
                 glyceryl monostearate 
                 5.0 
               
               
                 flavor 
                 suitable amount 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 buffer 
                 1.0 
               
               
                 purified water 
                 remainder 
               
               
                 Example 4  Lotion (I) 
               
               
                 N-(2-hydroxybenzyl)-L-arginineethylester 
                  3.0% by weight 
               
               
                 glycerol 
                 3.0 
               
               
                 sorbitol 
                 2.0 
               
               
                 polyoxyethylene(20)oleylether 
                 1.0 
               
               
                 ethanol 
                 15.0 
               
               
                 zinc p-phenolsulfonate 
                 0.2 
               
               
                 buffer 
                 0.1 
               
               
                 flavor 
                 0.2 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 5  Lotion (II) 
               
               
                 N-(2-hydroxybenzyl)-L-tyrosineethylester 
                  0.5% by weight 
               
               
                 glycerol 
                 4.0 
               
               
                 kaolin 
                 1.0 
               
               
                 calamine 
                 0.7 
               
               
                 camphor 
                 0.2 
               
               
                 ethanol 
                 14.0 
               
               
                 flavor 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 6  Cream 
               
               
                 N-(2-hydroxybenzyl)glycinelaurylester 
                  1.0% by weight 
               
               
                 kojic acid 
                 1.0 
               
               
                 stearic acid 
                 2.0 
               
               
                 polyoxyethylene(25)cetylether 
                 3.0 
               
               
                 glycerylmonostearate 
                 2.0 
               
               
                 octyldodecanol 
                 10.0 
               
               
                 cetanol 
                 6.0 
               
               
                 reduced lanolin 
                 4.0 
               
               
                 squalane 
                 9.0 
               
               
                 1,3-butylene glycol 
                 6.0 
               
               
                 polyethyleneglycol (1500) 
                 4.0 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 flavor 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 7  Cream 
               
               
                 N-(2-hydroxybenzyl)-L-leucinelaurylamide 
                  1.0% by weight 
               
               
                 solid paraffin 
                 5.0 
               
               
                 bees wax 
                 10.0 
               
               
                 vaseline 
                 15.0 
               
               
                 liquid paraffin 
                 41.0 
               
               
                 1,3-butyleneglycol 
                 4.0 
               
               
                 glycerolmonostearate 
                 2.0 
               
               
                 polyoxyethylenesorbitan(20)monolaurate 
                 2.0 
               
               
                 borax 
                 0.2 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 flavor 
                 suitable amount 
               
               
                 antioxidant 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 8  Milky lotion 
               
               
                 N-(2-hydroxybenzyl)glycineisopropylester 
                  2.0% by weight 
               
               
                 retinol 
                 0.1 
               
               
                 bees wax 
                 0.5 
               
               
                 vaseline 
                 2.0 
               
               
                 glycerylmonostearate 
                 1.0 
               
               
                 polyethyleneglycolmonooleate 
                 1.0 
               
               
                 methylpolysiloxane 
                 2.0 
               
               
                 cetanol 
                 1.0 
               
               
                 squalane 
                 6.0 
               
               
                 carboxyvinylpolymer 
                 0.5 
               
               
                 1,3-butyleneglycol 
                 4.0 
               
               
                 ethanol 
                 5.0 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 flavor 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 9  Milky lotion 
               
               
                 N-(2-hydroxybenzyl)-D-alaninelaurylamide 
                  1.0% by weight 
               
               
                 stearylalcohol 
                 0.5 
               
               
                 hardened palm oil 
                 3.0 
               
               
                 liquid paraffin 
                 35.0 
               
               
                 dipropyleneglycol 
                 6.0 
               
               
                 polyethyleneglycol (400) 
                 4.0 
               
               
                 sorbitan sesquioleate 
                 1.6 
               
               
                 polyoxyethylene(20)oleylether 
                 2.4 
               
               
                 carboxyvinylpolymer 
                 1.5 
               
               
                 potassium hydroxide 
                 0.1 
               
               
                 chelating agent 
                 suitable amount 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 flavor 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 10  Gel 
               
               
                 N-(2-hydroxybenzyl)-L-alanineoctylamide 
                 0.05% by weight 
               
               
                 liquid paraffin 
                 12.0 
               
               
                 glyceryl-tri(2-ethylhexanoate) 
                 50.0 
               
               
                 sorbitol 
                 10.0 
               
               
                 polyethyleneglycol(400) 
                 5.0 
               
               
                 acylmethyltaurine 
                 5.0 
               
               
                 polyoxyethylene(20)isocetylether 
                 10.0 
               
               
                 flavor 
                 suitable amount 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 11  Beauty lotion 
               
               
                 N-(2-hydoxybenzyl)-L-alanineisopropylester 
                  0.5% by weight 
               
               
                 dipropyleneglycol 
                 5.0 
               
               
                 polyethyleneglycol (400) 
                 5.0 
               
               
                 ethanol 
                 10.0 
               
               
                 carboxyvinylpolymer 
                 0.5 
               
               
                 sodium alginate 
                 0.5 
               
               
                 potassium hydroxide 
                 0.2 
               
               
                 polyoxyethylene(20)sorbitanmonostearate 
                 1.0 
               
               
                 sorbitolmonooleate 
                 0.5 
               
               
                 oleylalcohol 
                 0.5 
               
               
                 placenta extract 
                 0.2 
               
               
                 di-α-tocopherolacetate 
                 0.2 
               
               
                 flavor 
                 suitable amount 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 discoloration preventing agent 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 12  Pack 
               
               
                 N-(2-hydroxybenzyl)-L-phenylalaninelaurylester 
                  3.0% by weight 
               
               
                 polyvinylalcohol 
                 15.0 
               
               
                 carboxymethylcellulose 
                 5.0 
               
               
                 1,3-butyleneglycol 
                 5.0 
               
               
                 ethanol 
                 12.0 
               
               
                 polyoxyethylene(20)oleylether 
                 0.5 
               
               
                 flavor 
                 suitable amount 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 buffer 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 13  Foundation 
               
               
                 N-(2-hydroxybenzyl)glycinestearylamide 
                  5.0% by weight 
               
               
                 liquid paraffin 
                 10.0 
               
               
                 polyoxyethylene(20)sorbitan monooleate 
                 3.5 
               
               
                 propyleneglycol 
                 3.0 
               
               
                 titanium oxide 
                 9.0 
               
               
                 kaolin 
                 24.0 
               
               
                 talc 
                 42.0 
               
               
                 color pigment 
                 3.0 
               
               
                 flavor 
                 suitable amount 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 antioxidant 
                 suitable amount 
               
               
                 Example 14  Cleansing foam 
               
               
                 N-(2-hydroxybenzyl)-L-serine 
                  0.5% by weight 
               
               
                 N-lauroylglutamic acid triethanolamine salt 
                 25.0 
               
               
                 triethanolaminelaurate 
                 5.0 
               
               
                 polyoxyethylene(4)polyoxypropylene(11)butyl ether 
                 5.0 
               
               
                 ethanol 
                 3.0 
               
               
                 flavor 
                 suitable amount 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 15  Shampoo 
               
               
                 N-(2-hydroxybenzyl)-L-serineethylester 
                  0.5% by weight 
               
               
                 polyoxyethylene(3)laurylether triethanolaminesulfate 
                 3.0 
               
               
                 polyoxyethylene(3)laurylether sodium sulfate 
                 6.0 
               
               
                 sodium laurylsulfate 
                 1.5 
               
               
                 diethanolamidelaurate 
                 3.0 
               
               
                 lauryldimethylaminoacetic acid betaine 
                 2.5 
               
               
                 cationic cellulose 
                 0.2 
               
               
                 ethyleneglycoldistearate 
                 2.0 
               
               
                 flavor 
                 suitable amount 
               
               
                 antiseptic 
                 suitable amount 
               
               
                 chelating agent 
                 suitable amount 
               
               
                 buffer 
                 suitable amount 
               
               
                 purified water 
                 remainder 
               
               
                 Example 16  Bath cube (granules) 
               
               
                 N-(2-hydroxybenzyl)-L-histidineethylester 
                  3.0% by weight 
               
               
                 sodium sulfate 
                 44.0 
               
               
                 sodium hydrogencarbonate 
                 50.0 
               
               
                 borax 
                 2.0 
               
               
                 sodium carboxymethylcellulose 
                 1.0 
               
               
                 pigment 
                 suitable amount 
               
               
                 flavor 
                 suitable amount