Abstract:
The invention relates to the detection of biomolecules or analogs thereof in micro-arrays and the supports used for the micro-arrays. Methods for determining or testing binding of a first member molecule within an array or library of tentative first member binding molecules for binding with a second member binding molecule are disclosed. A support for a micro-array suitable for determining binding of a first member molecule within a library of spots of tentative first member binding molecules with a second member binding molecule is disclosed. The support includes a support surface wherein surface patches are interspersed within surface areas that are materially distinct from the patches.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS  
       [0001]    This application is a continuation of PCT/NL02/00097, filed Feb. 15, 2002, designating the United States of America, corresponding to PCT International Publication WO 02/066984 (published in English on Aug. 29, 2002), the contents of which are incorporated herein in its entirety. 
     
    
     
       TECHNICAL FIELD  
         [0002]    The invention relates to the detection of (bio)molecules, or analogues thereof, in micro-arrays and the supports used for the micro-arrays. More particularly, the invention relates to methods for determining or testing binding of a first member molecule within an array or a library of tentative first member binding molecules with a second member binding molecule, wherein the first and second molecules are each members of a binding pair. The invention further relates to enzyme-linked detection of the binding pair in high-density micro-array systems.  
         BACKGROUND  
         [0003]    Interactions, or the formation of a specific binding pair, between binding molecules, which in general are bio-molecules, and their corresponding ligands, which in general are also bio-molecules, are central to life. Cells often bear or contain receptor molecules that interact or bind with a hormone, a peptide, a drug, an antigen, an effector molecule or with another receptor molecule. Examples of binding pairs include: enzymes that bind with their respective substrate; antibody molecules that bind with an antigen; nucleic acids that bind with a protein, and so on. The terms “interact” or “bind” as used herein are meant to refer to the range of molecular forces between the binding molecule and ligand (or the functional parts thereof) as they approach each other, and may influence each other&#39;s properties. This approach takes the binding molecule and its ligand through various stages of molecular recognition comprising increasing degrees of intimacy and mutual effect, thus the two members bind and form a pair.  
           [0004]    Binding molecules have this binding ability because the binding molecules have distinct binding sites that allow for the recognition of the ligand in question. The ligand, in turn, has a corresponding binding site, and when the two binding sites interact—essentially spatial—complementarity, the two molecules can bind. Molecules typically have three dimensions and binding sites are often of a three dimensional nature, wherein one molecule includes one or more surface projections or protuberances as one binding site that corresponds to one or more pockets or depressions on the other binding molecule, such as a three-dimensional lock-and-key arrangement that may be an induced-fit variety.  
           [0005]    Due to the central role binding molecules and their ligands play in life, there is an ever expanding interest in testing for or identifying the nature or characteristics of the binding site and the members of the binding pair of molecules involved in such a site. Not only is one interested in the exact nature of the particular interaction between the binding molecule and ligand in question, e.g., in order to replace or supplement binding molecules or ligands when needed, one is also interested in knowing the approximating characteristics of the interaction in order to find, or design, analogs, agonists, antagonists or other compounds that mimic a binding site or ligand involved.  
           [0006]    Versatile and rapid methods to test for or identify binding pairs and its separate members exist. For instance, most, if not all nucleic acid detection techniques, and molecular libraries using these detection techniques entail hybridization of an essentially continuous nucleic acid stretch with a complementary nucleic acid strand, such as DNA, RNA or PNA. Proteins and peptides are often detected using antibodies, or derivatives or synthetic variants thereof. Arrays of biological molecules, i.e., micro-arrays, are used in standard techniques in many laboratories. Such micro-array-based detection generally includes a method in which a member of a specific binding pair is detected by means of an optically detectable reaction. Different supports for the libraries including tentative or possible first members of the binding pair (such as nucleic acid, peptide, or of any other nature) are used, but can be divided into two types: porous surfaces and non-porous surfaces.  
           [0007]    Porous surfaces, such as membranes, cellulose and paper, are probably the oldest support in use. For example, “dot blots” are widely used today. The synthesis of macromolecules, e.g., nucleic acids or peptides, has been described on these porous matrices. Paper was used as a relatively thick, continuous porous matrix on which first member constructs were synthesized spot wise. Binding pairs were generally identified by detection with, directly or via indirectly, enzyme labeled probes, thus, allowing increased sensitivity over the use of probes that were directly labeled with an optically detectable reporter molecule, such as a fluorescent group. A disadvantage of these methods is that the density of spots in these matrices is limited. This limitation is caused, inter alia, by the diffusion of the enzymatically changed substrate in the matrix. To avoid the diffusion and, thus, the inaccurate localization of a binding pair in the field of peptide synthesis, methods using polyethylene pins (Geysen, 1983) or in polypropylene wells (Slootstra, 1995; 1997) exist. However, these “early” methods have the disadvantage that no high density arrays (approximately up to not more than 10-20 spots/cm2) can be facilitated for various reasons.  
           [0008]    The existence of limited spot densities in the arrays is the reason why more nonporous surfaces are becoming more widely used. In the field of genomics, huge arrays of polynucleotide sequences are spotted on a variety of surfaces, typically glass slides covered with different coatings (See, U.S. Pat. Nos. 6,015,880 and 5,700,637). Array densities of 1000 spots/cm2 are possible. Even higher densities are possible when the biomolecules (i.e., polynucleotide sequences) are synthesized in-situ (See, U.S. Pat. No. 5,871,928). For example, in a traditional gene expression assay designed to profile the expression of many genes in parallel, mRNA is prepared from two different tissue types, e.g., normal and diseased samples. The isolated mRNA represents a snapshot of the current state of expression within the cells. The mRNA is converted to DNA via a first strand cDNA labeling reaction. After target DNA is deposited onto coated glass slides and directly labeled cDNA probes are hybridized to the arrays, the hybridized arrays are imaged using an array scanner and the results are examined for differences in expression levels using several image and data analysis software tools. A more intricate porous support surface has been described for these purposes (See, PCT International Publication WO 00/56934) that uses continuous porous matrix arrays. On microscope slides, a continuous slab of polyacrylamide is formed, e.g., 20 um thick and having a thin, continuous porous matrix (hydrogel) that is combined with a non porous surface (glass).  
           [0009]    Detection of specific binding pairs on or in the high-density supports is achieved with directly labeled probes that include optically detectable, e.g., fluorescent, nucleotides or antibodies. The detection techniques are highly sensitive, have low non-specific binding and high photo stability. Labeled nucleotides are widely used for labeling DNA and RNA probes, especially for multicolor analysis in micro-arrays, but also for FISH, chromosome identification, whole chromosome painting, karyotyping and gene mapping.  
           [0010]    Labeled nucleotides are available in a range of bright, intense colors having narrow emission bands that are ideal for multiplexing within a single sample. For protein or peptide detection, fewer fluorescently labeled probes are available since the field of protein or peptide based high-density micro-array systems is not as well developed as micro-arrays based on nucleic acid detection.  
         SUMMARY OF THE INVENTION  
         [0011]    In one exemplary embodiment, an array or library of first members is disclosed, in general spatially and/or addressable bound, most often covalently, to such a support, e.g., by spotting or gridding. A second member, the detecting or specific binding molecule, which is directly or indirectly labeled with a marker molecule (such as a fluorescent compound) to facilitate optical detection of the aforementioned pair is also disclosed to detect the putative first members of the array with which it can bind. The second member can of course be a nucleic acid, a receptor molecule, an antibody or the like. Binding of the second member, thus, identifies the first member because of its specific localization on the support.  
           [0012]    The invention combines the advantages of high density arraying, i.e., testing a lot of binding events at once, and enzyme-linked assays (very sensitive), thus, allowing for the detection of more binding pairs more rapidly. Micro-array systems are disclosed herein that work with enzyme-linked assays in order to detect the molecule of interest on a high-density support. Such testing of high densities of constructs on a solid support in a enzyme-linked assay is disclosed by the invention, wherein a first member is attached to or synthesized on one side of a surface of the support in a density of at least 25, at least 50, at least 100, about 200-500, or even 1000 spots per square centimeter.  
           [0013]    In one exemplary embodiment, the invention describes a support of polymeric material, i.e., a polymeric support, having a library of spots of the tentative first member binding molecules in a density of at least 25 spots, at least 50 spots, at least 100 spots, about 200-500 spots, or even 1000 spots per square centimeter. In another exemplary embodiment, the polymeric material comprises polypropylene having hydrophilic patches.  
           [0014]    The first binding pair members may be spotted or gridded in a positionally or spatially addressable way such that many different constructs or first member molecules on the support can interact with a second member or binding molecule. The spots can overlap, as long as the constituting collection of first member molecules are spatially addressable and distinct. Spotting can be done using piezo drop-on-demand technology or by using miniature solenoid valves. Gridding can be done using a set of individual needles that pick up sub-microliter amounts of segment solution from a microtiter plate, wherein the segment solution includes solutions having the first members.  
           [0015]    When peptides are tested, the support may be de-protected and extensively washed after the linking reaction to remove uncoupled peptides. The disclosed method gives a peptide construct density as large as 25 to 50, even 100 to 200, or up to 500 to 1000 spots per square centimeter. This density allows for the screening of a large number of possible peptide constructs of the proteins that bind with an antibody. For example, in one exemplary embodiment, 25,000 to 100,000 constructs are made on a 1000 square centimeter surface. The surface is screened for binding in an enzyme-linked assay, be it directly or indirectly, wherein a fluorescent substrate is generated with 100 ml of enzyme-labeled probe solution that contains 1-10 μg of probe/ml. The screened surface is developed with an optically detectable substrate using established techniques.  
           [0016]    The invention, thus, discloses a method for determining the binding of a first member molecule from a library of tentative first member binding molecules with a second member binding molecule. The method comprises providing a polymeric (such as the polypropylene type surface having hydrophilic patches) or plastic support with a library of spots of the tentative first member binding molecules in a density of at least 25 spots per square centimeter. The method further comprises detecting the binding of the first member molecule with the second member molecule with an enzyme-linked assay, wherein the enzyme-linked-assay comprises the production of a fluorescent or chemiluminescent substrate. Fluorescent substrates can be produced with a host of enzyme systems, such as horse-radish-peroxidase, alkaline phosphatase or other known substrate-enzyme systems such as those disclosed in Mendoza et al. including “High-throughput microarray-based enzyme-linked immunosorbent assay (ELISA),” Biotechniques, Eaton Publishing, Natick, US vol. 27, (1999), where an optically flat, glass-based support is described, including multitudes of identically patterned arrays of antigens printed to the glass.  
           [0017]    As described herein, indirect or direct fluorescence detection allocates antibody binding constructs. For instance, direct fluorescence detection with confocal scanning detection methods allows antibody detection on spots generated with droplets having peptide-solution in the sub-nanoliter range, which makes even higher construct densities feasible. Nucleic acid libraries may be made in a similar fashion using enzyme-labeled nucleic acid probes.  
           [0018]    Furthermore, the invention describes a support for a micro-array configured for testing binding of a first member molecule within an array or library of tentative first member binding molecules with a second member binding molecule. The support includes a surface having patches that are interspersed within areas, wherein the areas are materially distinct from the patches. In general, the surface is obtained using various complicated methods that comprise masking and subsequent photographic exposure and development (See, PCT International Publication WO 94/27719), plasma treatment, polymerization, photo-oxidation or electron beam treatment (See, WO 99/58245). Other techniques (See, Canadian Publication CA 2260807) require an inert solid support material to which the hydrophobic and hydrophilic areas need be applied, such as by way of coating. Other techniques (See, Great Britain Publication GB 2332273) provide an extremely hydrophobic surface in relation to the sample solution that is applied after which samples are thought to adhere to the surface by drying. In U.S. Pat. No. 5,369,102, a support with two opposing surfaces, one hydrophobic and the opposing one hydrophilic, are provided for the attachment of cells to the hydrophilic surface. PCT International Publication WO 99/32705 discloses various grafting protocols, but does not disclose requirements as to roughness of the surface, or to a pattern of hydrophilic and hydrophobic patches.  
           [0019]    The present invention has recognized that masking or coating is not required and that grafting surfaces, such as polypropylene, is suitable provided that at least one side of the surface of the starting material, such as a substantially flat surface of at least 0.5 square centimeters or at least 1 square centimeter, is configured with a substantial roughness characterized by elevations and depressions. The rough surface allows for the interspersed character of hydrophobic and hydrophilic patches to occur on the side or surface. The pattern of hydrophilic (hydrophilic matrices typically cause severe diffusion) and hydrophobic areas (which may block diffusion) as disclosed herein diminish diffusion, especially when the patches are smaller than the droplet size of dispensed material (spots), which are the smallest when the spot density is the highest.  
           [0020]    In one exemplary embodiment, the invention discloses a support, wherein the surface of the areas essentially comprises relatively hydrophobic polypropylene and the surface of the patches essentially comprise polypropylene having a relatively hydrophilic material, such a grafted polyacrylic acid. The support disclosed herein comprises at least a spot or dot, e.g. a collection of first member molecules such as a nucleic acid or peptide construct, density as large as 25, or up to 50, 100, 200, 500 or even 1,000 spots per square centimeter. Further, the spots or dots are positionally or spatially addressable, wherein each of the spot or dot covers at least one patch, but the spot or dot may cover from 3-5, or even from 5-15 or more hydrophilic patches or pixels.  
           [0021]    Although the surface, such as the polypropylene, may not be completely covered with a homogenous graft, the high loadings of peptide or nucleotide per square centimeter are possible due to the relatively high surface occupation of the grafts, such as polyacrylic acid, on the surface. Thicker grafts can carry higher peptide or nucleic acid loadings, but may suffer from more diffusion problems of dispensed material because of the growing occupation of grafted surface. Thus, the material can be made to suit various needs as regard to loading versus diffusion.  
           [0022]    The invention further discloses a solid support having at least one peptide, or at least one nucleotide. The solid support may include a plurality of peptides (or likewise of nucleotides), wherein the peptides or nucleotides are arranged in spots.  
           [0023]    In another exemplary embodiment, the invention describes a method for determining binding of a first member molecule within a library of tentative first member binding molecules with a second member binding molecule. The method includes providing at least one surface of a support having a library of spots of the tentative first member binding molecules, detecting the binding in an enzyme-linked assay and providing for limited, minimalized or restricted diffusion of an optically detectable marker molecule. Since diffusion is limited, the enzymatic reaction and the deposit or localization of the resulting (optically) detectable marker molecules can be determined with more precision and allow for higher densities than with previous micro-arrays using enzyme-linked-detection.  
           [0024]    In a further exemplary embodiment, the invention discloses a method where the diffusion is limited by providing at least one surface of the support having surface patches that are interspersed within surface areas, wherein the surface areas are materially distinct from the patches. (See, FIG. 1). The invention also provides a support (also referred to as a discontinuous matrix array or pixel array), wherein the support surface material is of a varied or discontinuous nature as regards to hydrophilicity. In one embodiment of a support having a high-density micro-array, patches of relative hydrophilicity are interspersed with areas of relative hydrophobicity. There does not need to be a sharp border between the patches and the surrounding area. It is sufficient when distinct material differences or discontinuities exist between the center of a patch and the middle line of a surrounding area, wherein there is a more or less gradual material change in between.  
           [0025]    The patches and surrounding areas may be in a strict matrix or grid format, but this is not necessary. The patches are at least one or two dimensions smaller than the size of the circumference of the positioned droplets or spots of first member molecules that, in a later phase, will be provided to the support surface. Since the patches are smaller than the droplets or spots, at least 3-5 or at least 10-20 hydrophilic patches will fit within the circumference of a later spotted spot or droplet of the solution of a first member, whether the first member is a nucleic acid, a peptide, any other (bio-)molecule or combination thereof. The patches resemble pixels that, after a marker molecule has attached to a specific binding pair, create the optically detectable image, wherein a spot with a collection of first member molecules bound to second member molecules is detected.  
           [0026]    In another exemplary embodiment, a one-to-one fit of pixel or patch to droplet or spot is also feasible even when the patch is larger than a spot, but this not necessary. It is also not necessary to apply or provide the patches in a regular pattern. When a droplet or spot is provided, the interspersed hydrophobic character of the support surface will limit the diffusion of any aqueous solution. The diffusion of a solution of an optically detectable substrate (be it as precipitate or as solution) formed after the enzymatic reaction has taken place will also be limited in a later phase. The enzymatic reaction takes place when a first member is bound to a second member of a binding pair, wherein the presence of the relatively hydrophilic patch or patches within the droplet or spot circumference allows the substrate to be positioned, or detectable. The patches disclosed herein may also be described as pixels within the spot(s), wherein the optically detectable or fluorescent substrate will finally be located. If so desired, patches may be hydrophobic where the surrounding area is relatively hydrophilic, when, for example, solutions or (optically detectable) markers are tested of a more hydrophobic nature.  
           [0027]    In another exemplary embodiment, the support described herein comprises at least a spot or dot, e.g., a collection of first member molecules such as a nucleic acid or peptide construct, density as large as 25 or 50, even 100, 200, up to 500, or even 1,000 spots per square centimeter. The spots or dots are positionally or spatially addressable, wherein each of the spots or dots cover at least one patch, cover from 3-5 patches, or even 5-15 or more patches or pixels.  
           [0028]    Hydrophilic path sizes can be modified by selecting the appropriate support material, such as polyethylene, polypropylene or another relatively hydrophobic plastic material to begin with, or by providing the patch size with patches in the desired size, such as by utilizing print technology. For instance, a support surface may be produced from a relatively hydrophobic polypropylene surface upon which grafts are provided that form the relatively hydrophilic patches. The grafts are made with polyacrylic acid that has an excellent suitable hydrophilic nature and allows for testing under physiological circumstances. The patch size may be influenced by selecting the appropriate roughness or a polyethylene or polypropylene starting material. The roughness can also be modulated by sanding, polishing, any other mechanical (printing) or chemical (etching) method, or combinations thereof to modulate a surface on which the hydrophilic patches are to be generated. The smaller the hydrophilic patch size, the smaller the droplets can be applied, such as up to the size where at least one patch falls within the circumference of the applied droplets.  
           [0029]    A method for determining binding of a first member molecule within a library of tentative first member binding molecules with a second member binding molecule is also disclosed. The method includes providing a support with spots comprising the tentative first member binding molecules, providing a second member binding molecule and detecting binding of a first member molecule with the second member binding molecule.  
           [0030]    The binding is detected with an optically detectable marker, such as a fluorophore, that is directly or indirectly labeled to a probe. The probe may be a nucleic acid or an antibody and, thus, allows a support of the present invention to be used in any type of micro-array. By preventing diffusion, problems such as signal overload can be avoided or circumvented. Thus, one exemplary embodiment of the invention discloses a method wherein binding pairs are detected via enzyme-linked-assay techniques where diffusion or leakage can be a problem. By preventing diffusion, the enzymatic detection method disclosed herein is more sensitive and allows fewer copies of tentative first member molecules to be spotted on one spot, thus decreasing spot-size and increase spot density without losing sensitivity. The enzymatic detection may be up to 10-1,000 times more sensitive than detection using directly labeled probes.  
           [0031]    Suitable enzyme-substrate combinations and methods for use in exemplary embodiments of the invention are, for example, found with U.S. Pat. No. 4,931,223 that discloses processes in which light of different wavelengths is simultaneously released from two or more enzymatically decomposable chemiluminescent 1,2-dioxetane compounds. The compounds are configured by means of the inclusion of a different light emitting fluorophore in each compound, such that each compound emits light of the different wavelengths by decomposing each of the compounds with a different enzyme. Also, Bronstein et al., BioTechniques 12 #5 (May 1992) pp. 748-753, “Improved Chemiluminescent Western Blotting Procedure” discloses an assay method in which a member of a specific binding pair is detected with an optically detectable reaction. The reaction includes an enzyme of a dioxetane such that the enzyme cleaves an enzyme-cleavable group from the dioxetane to form a negatively charged substituent bonded to the dioxetane. The negatively charged substituent causes the dioxetane to decompose and form a luminescent substance. Cano et al., J. App. Bacteriology 72 (1992) discloses an example of nucleic acid hybridization with a fluorescent alkaline phosphatase substrate that can also be used in the present invention, and Evangelista et al., Anal. Biochem. 203 (1992) teaches alkyl- and aryl-substituted salicyl phosphates as detection reagents in enzyme amplified fluorescence DNA hybridization assays.  
           [0032]    As will be described herein, a fluorescent substrate for alkaline phosphatase-based detection of protein blots is used with fluorescence scanning equipment, such as Molecular Dynamics FluorImager or Storm instruments, generally known as Vistra ECF and typically deemed suitable for use in Western blotting, and dot and slot blotting applications. The enzymatic reaction of alkaline phosphatase dephosphorylates the ECF substrate to produce a fluorescent product which is detectable in a method of the invention. The invention also discloses a method wherein a substrate for evaluating glycosidic enzymes comprising a fluorescein derivative, such as disclosed in U.S. Pat. No. 5,208,148, is used. The substrate bears a lypophilic character and will reside in hydrophobic areas of the surface.  
           [0033]    Another exemplary embodiment of the invention discloses a synthetic molecule comprising a binding site, i.e., located on the detected first member molecule or derivatives thereof, or a binding molecule including a binding site identifiable or obtainable by a method according to the invention. In a further exemplary embodiment of the invention, the support or methods may be used for identifying or obtaining a synthetic molecule having a binding site, or for identifying or obtaining a binding molecule capable of binding to a binding site, and the use of such an obtained molecule for interfering with or effecting binding to a binding molecule. 
       
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0034]    [0034]FIG. 1. Surface structure of polyacrylic acid grafted polypropylene (PP).  
         [0035]    [0035]FIG. 2. ECF-substrate wettability of different surfaces.  
         [0036]    [0036]FIG. 3. Storm fluorescence signals of the binding of peptide nr 1, 2, 3 and 4 (y-axis) to Mab GO 1 using five different gridding pins (on X-axis diameter gridding pins). Four different peptide concentrations were spotted on three different grafts: 12/50 Ac, 9/30 Ac and 6/12Ac grafts.  
         [0037]    FIGS.  4 A- 4 D. Maximal fluorescent signals of the spots as detected by the Storm of the binding of Mab GO1 to the peptide nr 1 (SEQ ID NO: 2), 2 (SEQ ID NO: 3), 3 (SEQ ID NO: 4) and 4 (SEQ ID NO: 5) on graft 6/12Ac using four different peptide concentrations and five different gridding pins are shown. Peptide concentrations: FIG. 4A: 1 mg/ml; FIG. 4B: 0.2 mg/ml; FIG. 4C: 0.04 mg/ml and FIG. 4D: 0.008 mg/ml.  
         [0038]    FIGS.  5 A- 5 C. Maximal fluorescent signals of the spots as detected by the Storm of the binding of Mab GO 1 to peptides nr 1 (SEQ ID NO: 2), 2 (SEQ ID NO: 3), 3 (SEQ ID NO: 4) and 4 (SEQ ID NO: 5) are shown. (Peptide concentration 0.2 mg/ml) on graft 6/12Ac, 9/30Ac and 12/50Ac. FIG. 5A: graft 6/12Ac; FIG. 5B: graft 9/30Ac; FIG. 5C: graft 12/5OAc.  
         [0039]    [0039]FIG. 6A. Schematic presentation of a head-to-tail complete matrix scan. 12345678901 and ABCDEFGHIJK represent sequences derived from a protein and a schematic presentation of a tail-to-tail complete matrix scan. This scan is similar to the scan shown in FIG. 4, however, the cysteine residue is positioned at the N-terminus of the second building block, leading to a reversed or tail-to-tail orientation of both building blocks. Both sequences are linked as previously described. In this scan, both sequences are shifted independently through the complete protein sequence, generating a library of all possible sequence combinations.  
         [0040]    [0040]FIG. 6B. List of all peptides (derived from hFSH) containing an N-terminal bromoacetamide group. Peptides 1) through 181) of FIG. 6B correspond to SEQ ID NOS: 6-186, respectively.  
         [0041]    [0041]FIG. 6C. List of all peptides (derived from hFSH) containing a C- or N-terminal cysteine. Peptides 1) through 7) correspond to SEQ ID NO: 187-193, respectively; peptide 8) corresponds to SEQ ID NO: 12; peptides 9) through 16) correspond to SEQ ID NOS: 194-201, respectively; peptide 17) corresponds to SEQ ID NO: 22; peptides 18) through 20) correspond to SEQ ID NOS: 202-204, respectively; peptide 21) corresponds to SEQ ID NO: 26; peptides 22) through 31) correspond to SEQ ID NOS: 205-214, respectively; peptide 32) corresponds to SEQ ID NO: 36; peptides 33) through 48) correspond to SEQ ID NOS: 215-230, respectively; peptide 49) corresponds to SEQ ID NO: 54; peptides 50) through 59) correspond to SEQ ID NOS: 231-240, respectively; peptide 60) corresponds to SEQ ID NO: 64; peptides 61) through 70) correspond to SEQ ID NOS: 241-250, respectively; peptide 71) corresponds to SEQ ID NO: 76; peptide 72) corresponds to SEQ ID NO: 251; peptide 73) corresponds to SEQ ID NO: 78; peptides 74) through 85) correspond to SEQ ID NOS: 252-263, respectively; peptide 86) corresponds to SEQ ID NO: 89; peptides 87) through 90) correspond to SEQ ID NOS: 264-267, respectively; peptide 91) corresponds to SEQ ID NO: 95; peptides 92) through 98) correspond to SEQ ID NOS: 268-274, respectively; peptides 99) and 100) both correspond to SEQ ID NO: 103; peptides 101) and 102) correspond to SEQ ID NOS: 275-276, respectively; peptide 103) corresponds to SEQ ID NO: 107; peptides 104) through 133) correspond to SEQ ID NOS: 277-306, respectively; peptides 134) corresponds to SEQ ID NO: 137; peptides 135) and 136) correspond to SEQ ID NOS: 307 and 308, respectively; peptide 137) corresponds to SEQ ID NO: 141; peptides 138) through 152) correspond to SEQ ID NOS: 309-323, respectively; peptide 153) corresponds to SEQ ID NO: 157; peptide 154) corresponds to SEQ ID NO: 324; peptide 155) corresponds to SEQ ID NO: 159; peptides 156) through 164) correspond to SEQ ID NOS: 325-333, respectively; peptide 165) corresponds to SEQ ID NO: 169; peptides 166) through 169) correspond to SEQ ID NOS: 334-337, respectively; peptide 170) corresponds to SEQ ID NO: 173; peptides 171) through 174) corresponds to SEQ ID NOS: 338-341, respectively; peptide 175) corresponds to SEQ ID NO: 179; and peptides 176) through 183) correspond to SEQ ID NOS: 342-349, respectively.  
         [0042]    [0042]FIG. 6D. Complete matrix scan, i.e., after coupling of all?ALL in B listed in B sequences to all? in C listed in C sequences, exemplified by cards 145-155 and a full picture of all binding values of all ca. 40,000 peptides (below). Peptide VYETVRVPGCAC$ADSLYTYPVATQ corresponds to SEQ ID NO: 350. The $ symbol refers to a bromoacetamide group.  
         [0043]    [0043]FIG. 7A. Schematic presentation of a multi-building block scan. 12345678901 (building block 1), NOPQRSTUVWXY (building block 2) and BCDEFGHIJKLM (building block 3) represent successive sequences derived from a protein. Building blocks were linked via a thioether bridge, formed by reaction of a free thiol function of a C-terminal cysteine residue (C) in building block 1 and a bromoacetamide group ($) at the N-terminus of building block 2 and so on, as described in example 3. In this scan all sequences are subsequently shifted simultaneously through the complete protein sequence to obtain the complete library.  
         [0044]    [0044]FIG. 7B. Working example obtained with an anti-hFSH monoclonal antibody-02.  
         [0045]    [0045]FIG. 7C. Binding values and list of peptides coupled onto each other. Peptides 1 through 36 correspond to SEQ ID NOS: 351-386, respectively. Card 6 is associated with SEQ ID NO: 356, card 07 is associated with SEQ ID NO: 357, card 08 is associated with SEQ ID NO: 358; card 09 is associated with SEQ ID NO: 359; card 0 is associated with SEQ ID NO: 360; card 11 is associated with SEQ ID NO: 361; card 12 is associated with SEQ ID NO: 362; card 13 is associated with SEQ ID NO: 363; card 1 is associated with SEQ ID NO: 371; card 22 is associated with SEQ ID NO: 372; card 35 is associated with SEQ ID NO: 385; and card 36 is associated with SEQ ID NO: 386.  
         [0046]    [0046]FIG. 7D. One square in full detail. The peptide $CKELVYETVRVPG (SEQ ID NO: 1) was coupled to the cysteine of card 06, which is associated with SEQ ID NO: 356. The $ symbol refers to a bromoacetamide group. To this card, peptides 1 to 36 were spotted with gridding pins. The binding values are shown below. Chemistry in short: Polypropylene (PP) surface was gamma irradiated (in this case 50kGy) in the presence of CuSO 4  and (in this case 12%) acrylic acid. Carboxylic acid functionalized PP was treated with Boc-HMDA/DCC/HOBt and subsequent treatment with trifluoracetic acid (TFA) yielded a surface with amino groups. To this amino group functionalized PP surface, N-Fmoc-S-trityl-L-cysteine (Fmoc-Cys-(Trt)-OH) was coupled using DCC and HOBt. Subsequently the Fmoc group was removed, followed by acetylation of amino group. Treatment of the surface with TFA (with triethylsilan and water as scavengers) yielded a thiol functionalized surface. Bromoacetyl (or other thiol reactive) containing peptides were allowed to react with the thiol groups of the PP surface in 0.015 M NaHCO 3  (pH 7-8, overnight reaction). Subsequently, the StBu groups (of the S-t-butylthio protected Cys residues) of the coupled peptides were removed using NaBH 4  (14 mg/ml in 0.015 M NaHCO 3  pH 7-8, 30 min, 30° C.), generating new thiol groups in the peptides. A second set of Bromoacetyl (or other thiol reactive) containing peptides were allowed to couple to the first set, making peptide constructs. This process can be repeated several times using different sets of bromoacetylated peptides. Peptides 1 through 36 corresponds to SEQ ID NOS: 351-386, respectively.  
         [0047]    [0047]FIG. 8. Storm fluorescence signals of the binding of Glu-ox to Mab GO1 on 3 different grafts using five different gridding pins.  
         [0048]    [0048]FIG. 9. On a matrix-scan of human Follicle-Stimulating Hormone (hFSH), the polyclonal anti-hFSH serum R5125 (Biotrent 4560-5215) was tested at 1 ug/ml. The matrix included four large squares (left side of the picture). Each large square contains 48 smaller squares. To the thiol group functionalized surfaces of each of these 48 squares (on all the four plates), one bromoacetylated hFSHpeptide (or a control peptide) is coupled via its bromoacetyl groups as described herein. In this way, each of the overlapping 13-mer peptides covering hFSH are coupled, generating 181 overlapping hFSH peptide functionalized squares+11 control peptide squares. All peptides possess a cysteine with a thiol protecting tert-butylsulfenyl group (Cys(StBu)). The same set of bromoacetylated hFSH peptides can be coupled to each peptide functionalized small square when the protecting StBu groups of the peptides on the peptide functionalized surfaces is removed by treatment of NaBH4 in aqueous environment at pH 7-8. Within each peptide functionalized square all bromoacetylated hFSH overlapping peptides are spotted generating, after coupling, 181 26-mer hFSH peptide constructs (spots) within each peptide functionalized square. In this way a matrix-scan is generated of all 32,761 (181*181) overlapping FSH 26-mer peptide constructs. The position of the cysteine (Cys(StBu)) in the peptides, used for coupling, varies. Peptide 1 (first 13-mer of hFSH=1-12Cys) has a Cys(StBu) on its C-terminal end, peptide 2 (peptide Cys2-13 of hFSH) contains a Cys(StBu) on the N-terminal site of the peptide while peptide 3 (peptide 3-14Cys of hFSH) again has a Cys(StBu) on its C-terminal end. Peptide 4 (peptide Cys4-15 of hFSH) has again an N-terminal Cys(StBu) and so on. Peptide 1 is coupled to the left top small square of the left top large square, peptide 2 is coupled to the left top small square, one step to the right, of the left top large square, peptide 3 is coupled to the left top small square, two steps to the right, of the left top large square and so on. The two enlarged squares on the right side of the figure show binding of antibody R5125 to peptide constructs on peptide functionalized square no.150 (upper enlarged square=peptide 150-162Cys of hFSH) and peptide functionalized square no.66 (lower enlarged square=peptide Cys66-78 of hFSH). A black color represents binding of antibody to peptide (black square) or peptide constructs (black spots). In the lower enlarged square, the first spot (left top) indicates binding of the antibody to a control peptide construct, the next spot to the right represents binding to a peptide construct containing peptide no. 1 (hFSH 1-12Cys(StBu)) coupled to hFSH Cys66-78 in lower enlarged square, again one spot to the right shows binding of the antibody to peptide construct hFSH Cys(StBU)2-13 with hFSH Cys66-78 and so on. White spots represent less binding of the antibody to the peptide construct compared to the binding of the antibody to the peptide within the square. No visible spots represent equal binding of the antibody to the peptide constructs compared to the binding of the peptide within the squares. 
     
    
     DETAILED DESCRIPTION  
       [0049]    The micro-array support and methods of the present invention may be used to detect binding of peptides, nucleic acids or other biomolecules. Conventional Pepscan methods use pins (Geysen et al.) or wells (Slootstra et al.). Polyacrylic acid grafts or other acrylic grafts on the polyethylene pins or in the polypropylene wells were used as carriers of peptides. Due to the high peptide loadings (each other carbon atom of the polymer can in theory carry a peptide) tested in an ELISA format, extreme low binding-interactions of a peptide to an antibody can be detected (detection of kD&lt;3 M are possible). In this system, the interactions were separated physically, i.e., by walls of wells. Technically, miniaturization of this concept stops at approximately 10 wells/cm 2  due to the limitations of conventional (syringe/needle) liquid handling techniques in practice. When the set-up is miniaturized, it is desirable to keep the two strongholds (high peptide loadings in combination with enzyme-linked detection methods) intact.  
         [0050]    Rough polypropylene (PP) supports are commercially available and are widely used as non-shiny material in all sorts of applications. The rough PP appeared to be an ideal template for attaching polyacrylic acid grafts. For example, microscope viewing of PP (EVACAST 1070 N16; Vink Kunststoffen BV) surfaces reveal rounded elevations (hills) separated by tiny depressions (valleys) (See, FIG. 1). The PP surface on top of the hills is relatively rough compared to the surface of valleys between the hills. The rough surface appeared to be a good scaffold for attaching grafts whereas the depressions accept grafts less readily. Thus, during grafting procedures using gamma irradiation, the graft is not regularly dispersed along the surface, but is deposited in patches surrounded by materially different areas corresponding to the depressions in the material. For example, using CuSO 4  and acrylic acid during grafting, most of the polyacrylic acid polymers are grafted on the top of the elevations and less in the depressions (See, FIG. 1). Thus, a more-or-less regular pattern of hydrophilic (polyacrylic acid grafts) patches and relatively hydrophobic (places without or less polyacrylic acid grafts) areas are present on the grafted PP surface.  
         [0051]    The pattern of hydrophilic (normally hydrophilic matrixes cause severe diffusion) and hydrophobic areas (blocks diffusion) diminish diffusion, especially when the patches are smaller then the droplet size of dispensed material. Although the surface of the PP is not completely covered with a homogenous graft, high loadings of peptide/cm 2  are possible due to the relatively high surface occupation of the polyacrylic acid grafts on the PP surfaces. In the above described setup, thicker grafts can carry higher peptide loadings, but will suffer from more diffusion problems of dispensed material because of the growing occupation of grafted surface. However, the material can be made to suit various needs as regard to loading versus diffusion.  
         [0052]    Enzyme-linked assays use substrates which are converted by the enzyme in products that precipitate in situ or are water soluble. A drawback of precipitating products is the non-reusability of the system caused by insolubility of the precipitated material during cleaning. The set up that makes use of non precipitating products, in particular non precipitating products which are fluorescent, because of the ease of detection by modern fluorescent signal detecting applications is desirable. When substrates (developing soluble products) are put on the surface, such as where excess of substrate material is in a later stage removed from the surface, dye development does not suffer from diffusion problems. This phenomenon is caused by the valley/hill or hydrophobic/hydrophilic construction of the surface in combination with excellent wettability properties of the polyacrylic acid matrix.  
         [0053]    [0053]FIG. 2 shows the Vistra ECF (2′(2-benzthiazoyl}-6′-hydroxy-benzthiozole phosphate bis-(2-amino-2-methyl-1,3-propanediol) salt; Amersham Pharmacia Biotech) substrate wettability of i) with and ii) without poly acrylic acid grafted PP (EVACAST 1070 N16; Vink Kunststoffen BV) and iii) CMT-glass slides (Corning) as detected on a Storm Fluorimager (Molecular Dynamics). Although the polyacrylic acid grafted PP-EVACAST surface is not continuously occupied with porous (polyacrylic acid grafts) material, the Storm Fluorimager does not detect irregular surface patterns. This is in contrast to un-grafted PP-EVACAST or CMT-glass slides.  
       EXAMPLES  
     Example 1  
       [0054]    A polypropylene (PP) support (EVACAST 1070 N16; Vink Kunststoffen BV) was grafted with acrylic acid to introduce polyacrylic acid grafts on the PP surface. In this case, the solid PP support was irradiated in the presence of 6%, 9% or 12% acrylic acid solutions in water containing CuSO4 using gamma radiation at a dose of 12, 30 or 50 kGy (combinations: 6% acrylic acid and 12kGy=6/12Ac; 9% acrylic acid with 30 kGy=9/30Ac and 12% acrylic acid with 50 kGy=12/50Ac). The grafted solid support containing carboxylic acid groups was functionalized with amino groups via coupling of t-butyloxycarbonylhexamethylenediamine (Boc-HMDA) using dicyclohexylearbodiimide (DCC) with N-hydroxybenztriazole (HOBt) and subsequent cleavage of the Boc groups using trifluoracetic acid. To introduce a thiol reactive bromacetamide group on the support, the amino group functionalized support was treated with bromoacetic acid using DCC or DCC/HOBt.  
         [0055]    Peptides containing cysteine residues were able to couple to the bromo functionalized surface via the thiol group of the cysteine residues forming a stable thioether bond. Peptides were spotted on the bromo functionalized surface using gridding pins (Genomic Solutions) with different diameters (1.5 mm, 0.8 mm, 0.6 mm, 0.4 mm and 0.25 mm). Solutions with different concentrations of peptide were used (1 mg/ml, 0.2 mg/ml, 0.04 mg/ml and 0.008 mg/ml). When aliquots of peptide solutions (in bicarbonate buffer at about pH 7-8) were dispensed on the support using the gridding pins, the coupling of the bromo group on the surface to the thiol group of the peptide was achieved in a humid chamber (overnight reaction). Extensive washing removed uncoupled peptide.  
         [0056]    Peptides used included: GCASLQGMDTCGK (Nr1) (SEQ ID NO: 2), CAFKQGVDTCGK (Nr2) (SEQ ID NO: 3), APDPFQGVDTCGK (Nr3) (SEQ ID NO: 4), and GCAPDPFQGVDTCGK (Nr4) (SEQ ID NO: 5). From surface plasmon resonance (SPR) measurements, affinity constants are known with antibody Mab GO1:Nr1 kD=&lt;10-3; Nr2 kD=3.10-7; Nr3 kD=4.10-6; and Nr4 kD=6.10-8.  
         [0057]    Binding of the antibody to the peptides was detected using a method that made use of a fluorescent product. The whole PP support containing the peptide functionalized areas was incubated with the antibody (Mab GO1 5 ug/ml, incubation overnight). After washing, a subsequent incubation of a second anti-mouse antibody conjugated to alkaline phosphatase introduces, after binding of the Mab to the peptide, the enzyme alkaline phosphatase at the peptide functionalized surface (spots). After washing, the bound enzyme caused fluorescent product signals at the peptide functionalized surfaces when a thin film of a Vistra ECF substrate (Amersham Pharmacia Biotech) solution was added to the surface (excess substrate was removed). Fluorescent product signals could be quantified on a Storm (Molecular Dynamics) in blue fluorescent mode.  
         [0058]    [0058]FIG. 3 shows the Storm fluorescent signals of the binding of the peptides Nr 1 (SEQ ID NO: 2), 2 (SEQ ID NO: 3), 3 (SEQ ID NO: 4) and 4 (SEQ ID NO: 5) to Mab GO1 using five different gridding pins and four different peptide concentrations on 3 different grafts. FIGS.  4 A- 4 D show the maximal fluorescent signals of the spots on graft 6/12Ac. FIG. 5 shows the maximal fluorescent signals of peptides Nr 1 (SEQ ID NO: 2), 2 (SEQ ID NO: 3), 3 (SEQ ID NO: 4) and 4 (SEQ ID NO: 5) spotted with 0.2 mg/ml on graft 6/12Ac, 9/30Ac and 12/50Ac.  
       Example 2  
       [0059]    Glucose Oxidase. A polypropylene (PP) support (EVACAST 1070 N16; Vink Kunststoffen BV) was grafted with polyacrylic acid. The solid support was irradiated in the presence of 6% acrylic acid solution in water containing CuSO4 using gamma radiation at a dose of 12kGy. The grafted solid support containing carboxylic acid groups was functionalized with amino groups via coupling of t-butyloxycarbonylhexamethylenediamine (Boc-HMDA) using dicyclohexylcarbodiimide (DCC) with N-hydroxybenztriazole (HOBt) and subsequent cleavage of the Boc groups using trifluoracetic acid. To introduce a thiol reactive bromacetamide group on the support, the amino group functionalized support was treated with bromoacetic acid using DCC or DCC/HOBt.  
         [0060]    Glucose oxidase containing thiol-groups (Glu-ox-SH) was able to couple to the bromo functionalized surface. Thiol groups on Glucose oxidase (Glu-ox; 1 mg/ml) were introduced in 0.16 M borate buffer (pH 8) using 2-iminothiolane (5 times molar excess 2-iminothiolane over Glu-ox; 45 min at room temperature). Glu-ox-SH was spotted on the bromo functionalized surface using gridding pins (Genomic Solutions) with different diameters (1.5 mm, 0.8 mm, 0.6 mm, 0.4 mm and 0.25 mm). Concentration of Glu-ox-SH was 0.25 mg/ml. When aliquots of Glu-ox-SH solutions (in phosphate buffered saline (PBS), 1 mM Titriplex=EDTA at pH 7) were dispensed on the support using the gridding pins, the coupling of the bromo group of the surface to the thiol group of Glu-ox-SH was achieved in a humid chamber (overnight reaction). Extensive washing removed uncoupled Glu-ox-SH.  
         [0061]    Binding of an antibody (Mab G01) to Glu-ox was detected using a method that made use of a fluorescent product. The whole PP support containing the Glu-ox functionalized areas was incubated with the antibody GO1 (5 ug/ml). After washing, a subsequent incubation of a second anti mouse antibody conjugated to alkaline phosphatase introduces, after binding of the Mab to Glu-ox, the enzyme alkaline phosphatase at the Glu-ox functionalized surface (spots). After washing, the bound enzyme caused fluorescent product signals at the peptide functionalized surfaces when Vistra ECF substrate (Amersham Pharmacia Biotech) (excess substrate was removed) was introduced. Fluorescent product signals could be quantified on a Storm (Molecular Dynamics) in blue fluorescent mode. FIG. 8 shows the Storm fluorescent signals of the binding Glu-ox to Mab GO 1 using five different gridding pins and three different grafts.  
       Example-3A  
       [0062]    Head-to-tail matrix-scan. In a complete matrix-scan, the N-terminal sequence of, for instance, sequence (1-11) of a protein is linked as a building block with each overlapping peptide sequence of a complete scan of the same protein as shown in FIG. 6A. Sequence (2-12) is linked with the same set of overlapping sequences and so on. The link can be formed, for instance, by reaction of a cysteine at the C-terminus of the second building block with a bromoacetamide modified N-terminus of the first building block. This means that every combination of, for instance, undecapeptides from the protein sequence is synthesized on a separate, known, position of the solid support.  
       Example-3B  
       [0063]    (Type II): Tail-to-tail matrix-scan. This is the same scan as the complete matrix scan from Example 3A, however, in this scan, the cysteine of the second building block is located at its N-terminus and provides a reversed or tail-to-tail orientation of both building blocks in the construct as shown in FIG. 6A. Examples 3A and 3B are illustrated in FIGS. 6B, 6C and  6 D.  
       Example-4  
       [0064]    Multi building block scan. In this example, a thiol function is introduced on an amino-functionalized solid support. This can be made by a direct reaction of the amino groups with, for instance, iminothiolane, or by coupling of Fmoc-Cys(Trt)-OH, followed by Fmoc cleavage using piperidine, acetylation, and trityl deprotection using TFA/scavenger mixtures. This thiol-functionalized solid support can be reacted with, for instance, a bromoacetamide-peptide, containing a protected cysteine residue. After coupling of the first peptide, the cysteine can be deprotected using, for instance, a TFA/scavenger mixture. The formed free thiol group can be used to couple a second bromoacetamide-peptide, again containing a protected cysteine. This procedure can be repeated to make multi-building block constructs. Several types of scans, as described in the other examples, can be used in combination with this multi building block scan. In FIG. 7A, an example is shown for a three multi building block scan. A working example with two building block scans is illustrated in FIGS. 7B, 7C and  7 D.  
       REFERENCES  
       [0065]    Frank R. Strategies and techniques in simultaneous solid phase synthesis based on the segmentation of membrane type supports. Bioorganic and Medical Chemistry Letters, 1993 Vol. 3 Number 3 pages 425-430.  
         [0066]    Geysen H. M., Meloen R. H. and Barteling S. J. (1984). Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proc. Natl. Acad. Sci. USA 81, 3998-4002.  
         [0067]    Slootstra J. W., Puijk W. C. Ligtvoet G. J., Langeveld J. P. M. &amp; Meloen R. H. 1995. Structural aspects of antibody-antigen interaction revealed through small random peptide libraries. Molecular Diversity 1, 87-96.  
         [0068]    Slootstra J W, Puijk W C, Ligtvoet G J, Kuperus D, Schaaper W M M, Meloen R H. 1997. Screening of a small set of random peptides: A new strategy to identify peptides that mimic epitopes. J. Mol. Recog. 10:219-224.  
     
       
       
         1 
         
           
             386  
           
           
             1  
             14  
             PRT  
             artificial sequence  
             
               artificial peptide building block  
             
           
            1 

Xaa Cys Lys Glu Leu Val Tyr Glu Thr Val Arg Val Pro Gly 
1               5                   10 

 
           
             2  
             13  
             PRT  
             artificial sequence  
             
               artificial peptide Nr1  
             
           
            2 

Gly Cys Ala Ser Leu Gln Gly Met Asp Thr Cys Gly Lys 
1               5                   10 

 
           
             3  
             12  
             PRT  
             artificial sequence  
             
               Artificial peptide Nr2  
             
           
            3 

Cys Ala Phe Lys Gln Gly Val Asp Thr Cys Gly Lys 
1               5                   10 

 
           
             4  
             13  
             PRT  
             artificial sequence  
             
               artificial peptide Nr3  
             
           
            4 

Ala Pro Asp Pro Phe Gln Gly Val Asp Thr Cys Gly Lys 
1               5                   10 

 
           
             5  
             15  
             PRT  
             artificial sequence  
             
               artificial peptide Nr4  
             
           
            5 

Gly Cys Ala Pro Asp Pro Phe Gln Gly Val Asp Thr Cys Gly Lys 
1               5                   10                  15 

 
           
             6  
             12  
             PRT  
             Homo sapiens  
           
            6 

Ala Pro Asp Val Gln Asp Cys Pro Glu Cys Thr Leu 
1               5                   10 

 
           
             7  
             12  
             PRT  
             Homo sapiens  
           
            7 

Pro Asp Val Gln Asp Cys Pro Glu Cys Thr Leu Gln 
1               5                   10 

 
           
             8  
             12  
             PRT  
             Homo sapiens  
           
            8 

Asp Val Gln Asp Cys Pro Glu Cys Thr Leu Gln Glu 
1               5                   10 

 
           
             9  
             12  
             PRT  
             Homo sapiens  
           
            9 

Val Gln Asp Cys Pro Glu Cys Thr Leu Gln Glu Asn 
1               5                   10 

 
           
             10  
             12  
             PRT  
             Homo sapiens  
           
            10 

Gln Asp Cys Pro Glu Cys Thr Leu Gln Glu Asn Pro 
1               5                   10 

 
           
             11  
             12  
             PRT  
             Homo sapiens  
           
            11 

Asp Cys Pro Glu Cys Thr Leu Gln Glu Asn Pro Phe 
1               5                   10 

 
           
             12  
             12  
             PRT  
             Homo sapiens  
           
            12 

Cys Pro Glu Cys Thr Leu Gln Glu Asn Pro Phe Phe 
1               5                   10 

 
           
             13  
             12  
             PRT  
             Homo sapiens  
           
            13 

Pro Glu Cys Thr Leu Gln Glu Asn Pro Phe Phe Ser 
1               5                   10 

 
           
             14  
             12  
             PRT  
             Homo sapiens  
           
            14 

Glu Cys Thr Leu Gln Glu Asn Pro Phe Phe Ser Gln 
1               5                   10 

 
           
             15  
             12  
             PRT  
             Homo sapiens  
           
            15 

Cys Thr Leu Gln Glu Asn Pro Phe Phe Ser Gln Pro 
1               5                   10 

 
           
             16  
             12  
             PRT  
             Homo sapiens  
           
            16 

Thr Leu Gln Glu Asn Pro Phe Phe Ser Gln Pro Gly 
1               5                   10 

 
           
             17  
             12  
             PRT  
             Homo sapiens  
           
            17 

Leu Gln Glu Asn Pro Phe Phe Ser Gln Pro Gly Ala 
1               5                   10 

 
           
             18  
             12  
             PRT  
             Homo sapiens  
           
            18 

Gln Glu Asn Pro Phe Phe Ser Gln Pro Gly Ala Pro 
1               5                   10 

 
           
             19  
             12  
             PRT  
             Homo sapiens  
           
            19 

Glu Asn Pro Phe Phe Ser Gln Pro Gly Ala Pro Ile 
1               5                   10 

 
           
             20  
             12  
             PRT  
             Homo sapiens  
           
            20 

Asn Pro Phe Phe Ser Gln Pro Gly Ala Pro Ile Leu 
1               5                   10 

 
           
             21  
             12  
             PRT  
             Homo sapiens  
           
            21 

Pro Phe Phe Ser Gln Pro Gly Ala Pro Ile Leu Gln 
1               5                   10 

 
           
             22  
             12  
             PRT  
             Homo sapiens  
           
            22 

Phe Phe Ser Gln Pro Gly Ala Pro Ile Leu Gln Cys 
1               5                   10 

 
           
             23  
             12  
             PRT  
             Homo sapiens  
           
            23 

Phe Ser Gln Pro Gly Ala Pro Ile Leu Gln Cys Met 
1               5                   10 

 
           
             24  
             12  
             PRT  
             Homo sapiens  
           
            24 

Ser Gln Pro Gly Ala Pro Ile Leu Gln Cys Met Gly 
1               5                   10 

 
           
             25  
             12  
             PRT  
             Homo sapiens  
           
            25 

Gln Pro Gly Ala Pro Ile Leu Gln Cys Met Gly Cys 
1               5                   10 

 
           
             26  
             12  
             PRT  
             Homo sapiens  
           
            26 

Pro Gly Ala Pro Ile Leu Gln Cys Met Gly Cys Cys 
1               5                   10 

 
           
             27  
             12  
             PRT  
             Homo sapiens  
           
            27 

Gly Ala Pro Ile Leu Gln Cys Met Gly Cys Cys Phe 
1               5                   10 

 
           
             28  
             12  
             PRT  
             Homo sapiens  
           
            28 

Ala Pro Ile Leu Gln Cys Met Gly Cys Cys Phe Ser 
1               5                   10 

 
           
             29  
             12  
             PRT  
             Homo sapiens  
           
            29 

Pro Ile Leu Gln Cys Met Gly Cys Cys Phe Ser Arg 
1               5                   10 

 
           
             30  
             12  
             PRT  
             Homo sapiens  
           
            30 

Ile Leu Gln Cys Met Gly Cys Cys Phe Ser Arg Ala 
1               5                   10 

 
           
             31  
             12  
             PRT  
             Homo sapiens  
           
            31 

Leu Gln Cys Met Gly Cys Cys Phe Ser Arg Ala Tyr 
1               5                   10 

 
           
             32  
             12  
             PRT  
             Homo sapiens  
           
            32 

Gln Cys Met Gly Cys Cys Phe Ser Arg Ala Tyr Pro 
1               5                   10 

 
           
             33  
             12  
             PRT  
             Homo sapiens  
           
            33 

Cys Met Gly Cys Cys Phe Ser Arg Ala Tyr Pro Thr 
1               5                   10 

 
           
             34  
             12  
             PRT  
             Homo sapiens  
           
            34 

Met Gly Cys Cys Phe Ser Arg Ala Tyr Pro Thr Pro 
1               5                   10 

 
           
             35  
             12  
             PRT  
             Homo sapiens  
           
            35 

Gly Cys Cys Phe Ser Arg Ala Tyr Pro Thr Pro Leu 
1               5                   10 

 
           
             36  
             12  
             PRT  
             Homo sapiens  
           
            36 

Cys Cys Phe Ser Arg Ala Tyr Pro Thr Pro Leu Arg 
1               5                   10 

 
           
             37  
             12  
             PRT  
             Homo sapiens  
           
            37 

Cys Phe Ser Arg Ala Tyr Pro Thr Pro Leu Arg Ser 
1               5                   10 

 
           
             38  
             12  
             PRT  
             Homo sapiens  
           
            38 

Phe Ser Arg Ala Tyr Pro Thr Pro Leu Arg Ser Lys 
1               5                   10 

 
           
             39  
             12  
             PRT  
             Homo sapiens  
           
            39 

Ser Arg Ala Tyr Pro Thr Pro Leu Arg Ser Lys Lys 
1               5                   10 

 
           
             40  
             12  
             PRT  
             Homo sapiens  
           
            40 

Arg Ala Tyr Pro Thr Pro Leu Arg Ser Lys Lys Thr 
1               5                   10 

 
           
             41  
             12  
             PRT  
             Homo sapiens  
           
            41 

Ala Tyr Pro Thr Pro Leu Arg Ser Lys Lys Thr Met 
1               5                   10 

 
           
             42  
             12  
             PRT  
             Homo sapiens  
           
            42 

Tyr Pro Thr Pro Leu Arg Ser Lys Lys Thr Met Leu 
1               5                   10 

 
           
             43  
             12  
             PRT  
             Homo sapiens  
           
            43 

Pro Thr Pro Leu Arg Ser Lys Lys Thr Met Leu Val 
1               5                   10 

 
           
             44  
             12  
             PRT  
             Homo sapiens  
           
            44 

Thr Pro Leu Arg Ser Lys Lys Thr Met Leu Val Gln 
1               5                   10 

 
           
             45  
             12  
             PRT  
             Homo sapiens  
           
            45 

Pro Leu Arg Ser Lys Lys Thr Met Leu Val Gln Lys 
1               5                   10 

 
           
             46  
             12  
             PRT  
             Homo sapiens  
           
            46 

Leu Arg Ser Lys Lys Thr Met Leu Val Gln Lys Asn 
1               5                   10 

 
           
             47  
             12  
             PRT  
             Homo sapiens  
           
            47 

Arg Ser Lys Lys Thr Met Leu Val Gln Lys Asn Val 
1               5                   10 

 
           
             48  
             12  
             PRT  
             Homo sapiens  
           
            48 

Ser Lys Lys Thr Met Leu Val Gln Lys Asn Val Thr 
1               5                   10 

 
           
             49  
             12  
             PRT  
             Homo sapiens  
           
            49 

Lys Lys Thr Met Leu Val Gln Lys Asn Val Thr Ser 
1               5                   10 

 
           
             50  
             12  
             PRT  
             Homo sapiens  
           
            50 

Lys Thr Met Leu Val Gln Lys Asn Val Thr Ser Glu 
1               5                   10 

 
           
             51  
             12  
             PRT  
             Homo sapiens  
           
            51 

Thr Met Leu Val Gln Lys Asn Val Thr Ser Glu Ser 
1               5                   10 

 
           
             52  
             12  
             PRT  
             Homo sapiens  
           
            52 

Met Leu Val Gln Lys Asn Val Thr Ser Glu Ser Thr 
1               5                   10 

 
           
             53  
             12  
             PRT  
             Homo sapiens  
           
            53 

Leu Val Gln Lys Asn Val Thr Ser Glu Ser Thr Cys 
1               5                   10 

 
           
             54  
             12  
             PRT  
             Homo sapiens  
           
            54 

Val Gln Lys Asn Val Thr Ser Glu Ser Thr Cys Cys 
1               5                   10 

 
           
             55  
             12  
             PRT  
             Homo sapiens  
           
            55 

Gln Lys Asn Val Thr Ser Glu Ser Thr Cys Cys Val 
1               5                   10 

 
           
             56  
             12  
             PRT  
             Homo sapiens  
           
            56 

Lys Asn Val Thr Ser Glu Ser Thr Cys Cys Val Ala 
1               5                   10 

 
           
             57  
             12  
             PRT  
             Homo sapiens  
           
            57 

Asn Val Thr Ser Glu Ser Thr Cys Cys Val Ala Lys 
1               5                   10 

 
           
             58  
             12  
             PRT  
             Homo sapiens  
           
            58 

Val Thr Ser Glu Ser Thr Cys Cys Val Ala Lys Ser 
1               5                   10 

 
           
             59  
             12  
             PRT  
             Homo sapiens  
           
            59 

Thr Ser Glu Ser Thr Cys Cys Val Ala Lys Ser Tyr 
1               5                   10 

 
           
             60  
             12  
             PRT  
             Homo sapiens  
           
            60 

Ser Glu Ser Thr Cys Cys Val Ala Lys Ser Tyr Asn 
1               5                   10 

 
           
             61  
             12  
             PRT  
             Homo sapiens  
           
            61 

Glu Ser Thr Cys Cys Val Ala Lys Ser Tyr Asn Arg 
1               5                   10 

 
           
             62  
             12  
             PRT  
             Homo sapiens  
           
            62 

Ser Thr Cys Cys Val Ala Lys Ser Tyr Asn Arg Val 
1               5                   10 

 
           
             63  
             12  
             PRT  
             Homo sapiens  
           
            63 

Thr Cys Cys Val Ala Lys Ser Tyr Asn Arg Val Thr 
1               5                   10 

 
           
             64  
             12  
             PRT  
             Homo sapiens  
           
            64 

Cys Cys Val Ala Lys Ser Tyr Asn Arg Val Thr Val 
1               5                   10 

 
           
             65  
             12  
             PRT  
             Homo sapiens  
           
            65 

Cys Val Ala Lys Ser Tyr Asn Arg Val Thr Val Met 
1               5                   10 

 
           
             66  
             12  
             PRT  
             Homo sapiens  
           
            66 

Val Ala Lys Ser Tyr Asn Arg Val Thr Val Met Gly 
1               5                   10 

 
           
             67  
             12  
             PRT  
             Homo sapiens  
           
            67 

Ala Lys Ser Tyr Asn Arg Val Thr Val Met Gly Gly 
1               5                   10 

 
           
             68  
             12  
             PRT  
             Homo sapiens  
           
            68 

Lys Ser Tyr Asn Arg Val Thr Val Met Gly Gly Phe 
1               5                   10 

 
           
             69  
             12  
             PRT  
             Homo sapiens  
           
            69 

Ser Tyr Asn Arg Val Thr Val Met Gly Gly Phe Lys 
1               5                   10 

 
           
             70  
             12  
             PRT  
             Homo sapiens  
           
            70 

Tyr Asn Arg Val Thr Val Met Gly Gly Phe Lys Val 
1               5                   10 

 
           
             71  
             12  
             PRT  
             Homo sapiens  
           
            71 

Asn Arg Val Thr Val Met Gly Gly Phe Lys Val Glu 
1               5                   10 

 
           
             72  
             12  
             PRT  
             Homo sapiens  
           
            72 

Arg Val Thr Val Met Gly Gly Phe Lys Val Glu Asn 
1               5                   10 

 
           
             73  
             12  
             PRT  
             Homo sapiens  
           
            73 

Val Thr Val Met Gly Gly Phe Lys Val Glu Asn His 
1               5                   10 

 
           
             74  
             12  
             PRT  
             Homo sapiens  
           
            74 

Thr Val Met Gly Gly Phe Lys Val Glu Asn His Thr 
1               5                   10 

 
           
             75  
             12  
             PRT  
             Homo sapiens  
           
            75 

Val Met Gly Gly Phe Lys Val Glu Asn His Thr Ala 
1               5                   10 

 
           
             76  
             12  
             PRT  
             Homo sapiens  
           
            76 

Met Gly Gly Phe Lys Val Glu Asn His Thr Ala Cys 
1               5                   10 

 
           
             77  
             12  
             PRT  
             Homo sapiens  
           
            77 

Gly Gly Phe Lys Val Glu Asn His Thr Ala Cys His 
1               5                   10 

 
           
             78  
             12  
             PRT  
             Homo sapiens  
           
            78 

Gly Phe Lys Val Glu Asn His Thr Ala Cys His Cys 
1               5                   10 

 
           
             79  
             12  
             PRT  
             Homo sapiens  
           
            79 

Phe Lys Val Glu Asn His Thr Ala Cys His Cys Ser 
1               5                   10 

 
           
             80  
             12  
             PRT  
             Homo sapiens  
           
            80 

Lys Val Glu Asn His Thr Ala Cys His Cys Ser Thr 
1               5                   10 

 
           
             81  
             12  
             PRT  
             Homo sapiens  
           
            81 

Val Glu Asn His Thr Ala Cys His Cys Ser Thr Cys 
1               5                   10 

 
           
             82  
             12  
             PRT  
             Homo sapiens  
           
            82 

Glu Asn His Thr Ala Cys His Cys Ser Thr Cys Tyr 
1               5                   10 

 
           
             83  
             12  
             PRT  
             Homo sapiens  
           
            83 

Asn His Thr Ala Cys His Cys Ser Thr Cys Tyr Tyr 
1               5                   10 

 
           
             84  
             12  
             PRT  
             Homo sapiens  
           
            84 

His Thr Ala Cys His Cys Ser Thr Cys Tyr Tyr His 
1               5                   10 

 
           
             85  
             12  
             PRT  
             Homo sapiens  
           
            85 

Thr Ala Cys His Cys Ser Thr Cys Tyr Tyr His Lys 
1               5                   10 

 
           
             86  
             12  
             PRT  
             Homo sapiens  
           
            86 

Ala Cys His Cys Ser Thr Cys Tyr Tyr His Lys Ser 
1               5                   10 

 
           
             87  
             12  
             PRT  
             Homo sapiens  
           
            87 

Asn Ser Cys Glu Leu Thr Asn Ile Thr Ile Ala Ile 
1               5                   10 

 
           
             88  
             12  
             PRT  
             Homo sapiens  
           
            88 

Ser Cys Glu Leu Thr Asn Ile Thr Ile Ala Ile Glu 
1               5                   10 

 
           
             89  
             12  
             PRT  
             Homo sapiens  
           
            89 

Cys Glu Leu Thr Asn Ile Thr Ile Ala Ile Glu Lys 
1               5                   10 

 
           
             90  
             12  
             PRT  
             Homo sapiens  
           
            90 

Glu Leu Thr Asn Ile Thr Ile Ala Ile Glu Lys Glu 
1               5                   10 

 
           
             91  
             12  
             PRT  
             Homo sapiens  
           
            91 

Leu Thr Asn Ile Thr Ile Ala Ile Glu Lys Glu Glu 
1               5                   10 

 
           
             92  
             12  
             PRT  
             Homo sapiens  
           
            92 

Thr Asn Ile Thr Ile Ala Ile Glu Lys Glu Glu Cys 
1               5                   10 

 
           
             93  
             12  
             PRT  
             Homo sapiens  
           
            93 

Asn Ile Thr Ile Ala Ile Glu Lys Glu Glu Cys Arg 
1               5                   10 

 
           
             94  
             12  
             PRT  
             Homo sapiens  
           
            94 

Ile Thr Ile Ala Ile Glu Lys Glu Glu Cys Arg Phe 
1               5                   10 

 
           
             95  
             12  
             PRT  
             Homo sapiens  
           
            95 

Thr Ile Ala Ile Glu Lys Glu Glu Cys Arg Phe Cys 
1               5                   10 

 
           
             96  
             12  
             PRT  
             Homo sapiens  
           
            96 

Ile Ala Ile Glu Lys Glu Glu Cys Arg Phe Cys Ile 
1               5                   10 

 
           
             97  
             12  
             PRT  
             Homo sapiens  
           
            97 

Ala Ile Glu Lys Glu Glu Cys Arg Phe Cys Ile Ser 
1               5                   10 

 
           
             98  
             12  
             PRT  
             Homo sapiens  
           
            98 

Ala Ile Glu Lys Glu Glu Cys Arg Phe Cys Ile Ser 
1               5                   10 

 
           
             99  
             12  
             PRT  
             Homo sapiens  
           
            99 

Glu Lys Glu Glu Cys Arg Phe Cys Ile Ser Ile Asn 
1               5                   10 

 
           
             100  
             12  
             PRT  
             Homo sapiens  
           
            100 

Lys Glu Glu Cys Arg Phe Cys Ile Ser Ile Asn Thr 
1               5                   10 

 
           
             101  
             12  
             PRT  
             Homo sapiens  
           
            101 

Glu Glu Cys Arg Phe Cys Ile Ser Ile Asn Thr Thr 
1               5                   10 

 
           
             102  
             12  
             PRT  
             Homo sapiens  
           
            102 

Glu Cys Arg Phe Cys Ile Ser Ile Asn Thr Thr Trp 
1               5                   10 

 
           
             103  
             12  
             PRT  
             Homo sapiens  
           
            103 

Cys Arg Phe Cys Ile Ser Ile Asn Thr Thr Trp Cys 
1               5                   10 

 
           
             104  
             12  
             PRT  
             Homo sapiens  
           
            104 

Arg Phe Cys Ile Ser Ile Asn Thr Thr Trp Cys Ala 
1               5                   10 

 
           
             105  
             12  
             PRT  
             Homo sapiens  
           
            105 

Phe Cys Ile Ser Ile Asn Thr Thr Trp Cys Ala Gly 
1               5                   10 

 
           
             106  
             12  
             PRT  
             Homo sapiens  
           
            106 

Cys Ile Ser Ile Asn Thr Thr Trp Cys Ala Gly Tyr 
1               5                   10 

 
           
             107  
             12  
             PRT  
             Homo sapiens  
           
            107 

Ile Ser Ile Asn Thr Thr Trp Cys Ala Gly Tyr Cys 
1               5                   10 

 
           
             108  
             12  
             PRT  
             Homo sapiens  
           
            108 

Ser Ile Asn Thr Thr Trp Cys Ala Gly Tyr Cys Tyr 
1               5                   10 

 
           
             109  
             12  
             PRT  
             Homo sapiens  
           
            109 

Ile Asn Thr Thr Trp Cys Ala Gly Tyr Cys Tyr Thr 
1               5                   10 

 
           
             110  
             12  
             PRT  
             Homo sapiens  
           
            110 

Asn Thr Thr Trp Cys Ala Gly Tyr Cys Tyr Thr Arg 
1               5                   10 

 
           
             111  
             12  
             PRT  
             Homo sapiens  
           
            111 

Thr Thr Trp Cys Ala Gly Tyr Cys Tyr Thr Arg Asp 
1               5                   10 

 
           
             112  
             12  
             PRT  
             Homo sapiens  
           
            112 

Thr Trp Cys Ala Gly Tyr Cys Tyr Thr Arg Asp Leu 
1               5                   10 

 
           
             113  
             12  
             PRT  
             Homo sapiens  
           
            113 

Trp Cys Ala Gly Tyr Cys Tyr Thr Arg Asp Leu Val 
1               5                   10 

 
           
             114  
             12  
             PRT  
             Homo sapiens  
           
            114 

Cys Ala Gly Tyr Cys Tyr Thr Arg Asp Leu Val Tyr 
1               5                   10 

 
           
             115  
             12  
             PRT  
             Homo sapiens  
           
            115 

Ala Gly Tyr Cys Tyr Thr Arg Asp Leu Val Tyr Lys 
1               5                   10 

 
           
             116  
             12  
             PRT  
             Homo sapiens  
           
            116 

Gly Tyr Cys Tyr Thr Arg Asp Leu Val Tyr Lys Asp 
1               5                   10 

 
           
             117  
             12  
             PRT  
             Homo sapiens  
           
            117 

Tyr Cys Tyr Thr Arg Asp Leu Val Tyr Lys Asp Pro 
1               5                   10 

 
           
             118  
             12  
             PRT  
             Homo sapiens  
           
            118 

Cys Tyr Thr Arg Asp Leu Val Tyr Lys Asp Pro Ala 
1               5                   10 

 
           
             119  
             12  
             PRT  
             Homo sapiens  
           
            119 

Tyr Thr Arg Asp Leu Val Tyr Lys Asp Pro Ala Arg 
1               5                   10 

 
           
             120  
             12  
             PRT  
             Homo sapiens  
           
            120 

Thr Arg Asp Leu Val Tyr Lys Asp Pro Ala Arg Pro 
1               5                   10 

 
           
             121  
             12  
             PRT  
             Homo sapiens  
           
            121 

Arg Asp Leu Val Tyr Lys Asp Pro Ala Arg Pro Lys 
1               5                   10 

 
           
             122  
             12  
             PRT  
             Homo sapiens  
           
            122 

Asp Leu Val Tyr Lys Asp Pro Ala Arg Pro Lys Ile 
1               5                   10 

 
           
             123  
             12  
             PRT  
             Homo sapiens  
           
            123 

Leu Val Tyr Lys Asp Pro Ala Arg Pro Lys Ile Gln 
1               5                   10 

 
           
             124  
             12  
             PRT  
             Homo sapiens  
           
            124 

Val Tyr Lys Asp Pro Ala Arg Pro Lys Ile Gln Lys 
1               5                   10 

 
           
             125  
             12  
             PRT  
             Homo sapiens  
           
            125 

Tyr Lys Asp Pro Ala Arg Pro Lys Ile Gln Lys Thr 
1               5                   10 

 
           
             126  
             12  
             PRT  
             Homo sapiens  
           
            126 

Lys Asp Pro Ala Arg Pro Lys Ile Gln Lys Thr Cys 
1               5                   10 

 
           
             127  
             12  
             PRT  
             Homo sapiens  
           
            127 

Asp Pro Ala Arg Pro Lys Ile Gln Lys Thr Cys Thr 
1               5                   10 

 
           
             128  
             12  
             PRT  
             Homo sapiens  
           
            128 

Pro Ala Arg Pro Lys Ile Gln Lys Thr Cys Thr Phe 
1               5                   10 

 
           
             129  
             12  
             PRT  
             Homo sapiens  
           
            129 

Ala Arg Pro Lys Ile Gln Lys Thr Cys Thr Phe Lys 
1               5                   10 

 
           
             130  
             12  
             PRT  
             Homo sapiens  
           
            130 

Arg Pro Lys Ile Gln Lys Thr Cys Thr Phe Lys Glu 
1               5                   10 

 
           
             131  
             12  
             PRT  
             Homo sapiens  
           
            131 

Pro Lys Ile Gln Lys Thr Cys Thr Phe Lys Glu Leu 
1               5                   10 

 
           
             132  
             12  
             PRT  
             Homo sapiens  
           
            132 

Lys Ile Gln Lys Thr Cys Thr Phe Lys Glu Leu Val 
1               5                   10 

 
           
             133  
             12  
             PRT  
             Homo sapiens  
           
            133 

Ile Gln Lys Thr Cys Thr Phe Lys Glu Leu Val Tyr 
1               5                   10 

 
           
             134  
             12  
             PRT  
             Homo sapiens  
           
            134 

Gln Lys Thr Cys Thr Phe Lys Glu Leu Val Tyr Glu 
1               5                   10 

 
           
             135  
             12  
             PRT  
             Homo sapiens  
           
            135 

Lys Thr Cys Thr Phe Lys Glu Leu Val Tyr Glu Thr 
1               5                   10 

 
           
             136  
             12  
             PRT  
             Homo sapiens  
           
            136 

Thr Cys Thr Phe Lys Glu Leu Val Tyr Glu Thr Val 
1               5                   10 

 
           
             137  
             12  
             PRT  
             Homo sapiens  
           
            137 

Cys Thr Phe Lys Glu Leu Val Tyr Glu Thr Val Arg 
1               5                   10 

 
           
             138  
             12  
             PRT  
             Homo sapiens  
           
            138 

Thr Phe Lys Glu Leu Val Tyr Glu Thr Val Arg Val 
1               5                   10 

 
           
             139  
             12  
             PRT  
             Homo sapiens  
           
            139 

Phe Lys Glu Leu Val Tyr Glu Thr Val Arg Val Pro 
1               5                   10 

 
           
             140  
             12  
             PRT  
             Homo sapiens  
           
            140 

Lys Glu Leu Val Tyr Glu Thr Val Arg Val Pro Gly 
1               5                   10 

 
           
             141  
             12  
             PRT  
             Homo sapiens  
           
            141 

Glu Leu Val Tyr Glu Thr Val Arg Val Pro Gly Cys 
1               5                   10 

 
           
             142  
             12  
             PRT  
             Homo sapiens  
           
            142 

Leu Val Tyr Glu Thr Val Arg Val Pro Gly Cys Ala 
1               5                   10 

 
           
             143  
             12  
             PRT  
             Homo sapiens  
           
            143 

Val Tyr Glu Thr Val Arg Val Pro Gly Cys Ala His 
1               5                   10 

 
           
             144  
             12  
             PRT  
             Homo sapiens  
           
            144 

Tyr Glu Thr Val Arg Val Pro Gly Cys Ala His His 
1               5                   10 

 
           
             145  
             12  
             PRT  
             Homo sapiens  
           
            145 

Glu Thr Val Arg Val Pro Gly Cys Ala His His Ala 
1               5                   10 

 
           
             146  
             12  
             PRT  
             Homo sapiens  
           
            146 

Thr Val Arg Val Pro Gly Cys Ala His His Ala Asp 
1               5                   10 

 
           
             147  
             12  
             PRT  
             Homo sapiens  
           
            147 

Val Arg Val Pro Gly Cys Ala His His Ala Asp Ser 
1               5                   10 

 
           
             148  
             12  
             PRT  
             Homo sapiens  
           
            148 

Arg Val Pro Gly Cys Ala His His Ala Asp Ser Leu 
1               5                   10 

 
           
             149  
             12  
             PRT  
             Homo sapiens  
           
            149 

Val Pro Gly Cys Ala His His Ala Asp Ser Leu Tyr 
1               5                   10 

 
           
             150  
             12  
             PRT  
             Homo sapiens  
           
            150 

Pro Gly Cys Ala His His Ala Asp Ser Leu Tyr Thr 
1               5                   10 

 
           
             151  
             12  
             PRT  
             Homo sapiens  
           
            151 

Gly Cys Ala His His Ala Asp Ser Leu Tyr Thr Tyr 
1               5                   10 

 
           
             152  
             12  
             PRT  
             Homo sapiens  
           
            152 

Cys Ala His His Ala Asp Ser Leu Tyr Thr Tyr Pro 
1               5                   10 

 
           
             153  
             12  
             PRT  
             Homo sapiens  
           
            153 

Ala His His Ala Asp Ser Leu Tyr Thr Tyr Pro Val 
1               5                   10 

 
           
             154  
             12  
             PRT  
             Homo sapiens  
           
            154 

His His Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala 
1               5                   10 

 
           
             155  
             12  
             PRT  
             Homo sapiens  
           
            155 

His Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala Thr 
1               5                   10 

 
           
             156  
             12  
             PRT  
             Homo sapiens  
           
            156 

Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala Thr Gln 
1               5                   10 

 
           
             157  
             12  
             PRT  
             Homo sapiens  
           
            157 

Asp Ser Leu Tyr Thr Tyr Pro Val Ala Thr Gln Cys 
1               5                   10 

 
           
             158  
             12  
             PRT  
             Homo sapiens  
           
            158 

Ser Leu Tyr Thr Tyr Pro Val Ala Thr Gln Cys His 
1               5                   10 

 
           
             159  
             12  
             PRT  
             Homo sapiens  
           
            159 

Leu Tyr Thr Tyr Pro Val Ala Thr Gln Cys His Cys 
1               5                   10 

 
           
             160  
             12  
             PRT  
             Homo sapiens  
           
            160 

Tyr Thr Tyr Pro Val Ala Thr Gln Cys His Cys Gly 
1               5                   10 

 
           
             161  
             12  
             PRT  
             Homo sapiens  
           
            161 

Thr Tyr Pro Val Ala Thr Gln Cys His Cys Gly Lys 
1               5                   10 

 
           
             162  
             12  
             PRT  
             Homo sapiens  
           
            162 

Tyr Pro Val Ala Thr Gln Cys His Cys Gly Lys Cys 
1               5                   10 

 
           
             163  
             12  
             PRT  
             Homo sapiens  
           
            163 

Pro Val Ala Thr Gln Cys His Cys Gly Lys Cys Asp 
1               5                   10 

 
           
             164  
             12  
             PRT  
             Homo sapiens  
           
            164 

Val Ala Thr Gln Cys His Cys Gly Lys Cys Asp Ser 
1               5                   10 

 
           
             165  
             12  
             PRT  
             Homo sapiens  
           
            165 

Ala Thr Gln Cys His Cys Gly Lys Cys Asp Ser Asp 
1               5                   10 

 
           
             166  
             12  
             PRT  
             Homo sapiens  
           
            166 

Thr Gln Cys His Cys Gly Lys Cys Asp Ser Asp Ser 
1               5                   10 

 
           
             167  
             12  
             PRT  
             Homo sapiens  
           
            167 

Gln Cys His Cys Gly Lys Cys Asp Ser Asp Ser Thr 
1               5                   10 

 
           
             168  
             12  
             PRT  
             Homo sapiens  
           
            168 

Cys His Cys Gly Lys Cys Asp Ser Asp Ser Thr Asp 
1               5                   10 

 
           
             169  
             12  
             PRT  
             Homo sapiens  
           
            169 

His Cys Gly Lys Cys Asp Ser Asp Ser Thr Asp Cys 
1               5                   10 

 
           
             170  
             12  
             PRT  
             Homo sapiens  
           
            170 

Cys Gly Lys Cys Asp Ser Asp Ser Thr Asp Cys Thr 
1               5                   10 

 
           
             171  
             12  
             PRT  
             Homo sapiens  
           
            171 

Gly Lys Cys Asp Ser Asp Ser Thr Asp Cys Thr Val 
1               5                   10 

 
           
             172  
             12  
             PRT  
             Homo sapiens  
           
            172 

Lys Cys Asp Ser Asp Ser Thr Asp Cys Thr Val Arg 
1               5                   10 

 
           
             173  
             12  
             PRT  
             Homo sapiens  
           
            173 

Cys Asp Ser Asp Ser Thr Asp Cys Thr Val Arg Gly 
1               5                   10 

 
           
             174  
             12  
             PRT  
             Homo sapiens  
           
            174 

Asp Ser Asp Ser Thr Asp Cys Thr Val Arg Gly Leu 
1               5                   10 

 
           
             175  
             12  
             PRT  
             Homo sapiens  
           
            175 

Ser Asp Ser Thr Asp Cys Thr Val Arg Gly Leu Gly 
1               5                   10 

 
           
             176  
             12  
             PRT  
             Homo sapiens  
           
            176 

Asp Ser Thr Asp Cys Thr Val Arg Gly Leu Gly Pro 
1               5                   10 

 
           
             177  
             12  
             PRT  
             Homo sapiens  
           
            177 

Ser Thr Asp Cys Thr Val Arg Gly Leu Gly Pro Ser 
1               5                   10 

 
           
             178  
             12  
             PRT  
             Homo sapiens  
           
            178 

Thr Asp Cys Thr Val Arg Gly Leu Gly Pro Ser Tyr 
1               5                   10 

 
           
             179  
             12  
             PRT  
             Homo sapiens  
           
            179 

Asp Cys Thr Val Arg Gly Leu Gly Pro Ser Tyr Cys 
1               5                   10 

 
           
             180  
             12  
             PRT  
             Homo sapiens  
           
            180 

Cys Thr Val Arg Gly Leu Gly Pro Ser Tyr Cys Ser 
1               5                   10 

 
           
             181  
             12  
             PRT  
             Homo sapiens  
           
            181 

Thr Val Arg Gly Leu Gly Pro Ser Tyr Cys Ser Phe 
1               5                   10 

 
           
             182  
             12  
             PRT  
             Homo sapiens  
           
            182 

Val Arg Gly Leu Gly Pro Ser Tyr Cys Ser Phe Gly 
1               5                   10 

 
           
             183  
             12  
             PRT  
             Homo sapiens  
           
            183 

Arg Gly Leu Gly Pro Ser Tyr Cys Ser Phe Gly Glu 
1               5                   10 

 
           
             184  
             12  
             PRT  
             Homo sapiens  
           
            184 

Gly Leu Gly Pro Ser Tyr Cys Ser Phe Gly Glu Met 
1               5                   10 

 
           
             185  
             12  
             PRT  
             Homo sapiens  
           
            185 

Leu Gly Pro Ser Tyr Cys Ser Phe Gly Glu Met Lys 
1               5                   10 

 
           
             186  
             12  
             PRT  
             Homo sapiens  
           
            186 

Gly Pro Ser Tyr Cys Ser Phe Gly Glu Met Lys Glu 
1               5                   10 

 
           
             187  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            187 

Ala Pro Asp Val Gln Asp Cys Pro Glu Cys Thr Cys 
1               5                   10 

 
           
             188  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            188 

Cys Pro Asp Val Gln Asp Cys Pro Glu Cys Thr Leu 
1               5                   10 

 
           
             189  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            189 

Asp Val Gln Asp Cys Pro Glu Cys Thr Leu Gln Cys 
1               5                   10 

 
           
             190  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            190 

Cys Val Gln Asp Cys Pro Glu Cys Thr Leu Gln Glu 
1               5                   10 

 
           
             191  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            191 

Gln Asp Cys Pro Glu Cys Thr Leu Gln Glu Asn Cys 
1               5                   10 

 
           
             192  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            192 

Cys Asp Cys Pro Glu Cys Thr Leu Gln Glu Asn Pro 
1               5                   10 

 
           
             193  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            193 

Cys Pro Glu Cys Thr Leu Gln Glu Asn Pro Phe Cys 
1               5                   10 

 
           
             194  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            194 

Glu Cys Thr Leu Gln Glu Asn Pro Phe Phe Ser Cys 
1               5                   10 

 
           
             195  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            195 

Cys Cys Thr Leu Gln Glu Asn Pro Phe Phe Ser Gln 
1               5                   10 

 
           
             196  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            196 

Thr Leu Gln Glu Asn Pro Phe Phe Ser Gln Pro Cys 
1               5                   10 

 
           
             197  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            197 

Cys Leu Gln Glu Asn Pro Phe Phe Ser Gln Pro Gly 
1               5                   10 

 
           
             198  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            198 

Gln Glu Asn Pro Phe Phe Ser Gln Pro Gly Ala Cys 
1               5                   10 

 
           
             199  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            199 

Cys Glu Asn Pro Phe Phe Ser Gln Pro Gly Ala Pro 
1               5                   10 

 
           
             200  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            200 

Asn Pro Phe Phe Ser Gln Pro Gly Ala Pro Ile Cys 
1               5                   10 

 
           
             201  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            201 

Cys Pro Phe Phe Ser Gln Pro Gly Ala Pro Ile Leu 
1               5                   10 

 
           
             202  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            202 

Cys Phe Ser Gln Pro Gly Ala Pro Ile Leu Gln Cys 
1               5                   10 

 
           
             203  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            203 

Ser Gln Pro Gly Ala Pro Ile Leu Gln Cys Met Cys 
1               5                   10 

 
           
             204  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            204 

Cys Gln Pro Gly Ala Pro Ile Leu Gln Cys Met Gly 
1               5                   10 

 
           
             205  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            205 

Cys Gly Ala Pro Ile Leu Gln Cys Met Gly Cys Cys 
1               5                   10 

 
           
             206  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            206 

Ala Pro Ile Leu Gln Cys Met Gly Cys Cys Phe Cys 
1               5                   10 

 
           
             207  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            207 

Cys Pro Ile Leu Gln Cys Met Gly Cys Cys Phe Ser 
1               5                   10 

 
           
             208  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            208 

Ile Leu Gln Cys Met Gly Cys Cys Phe Ser Arg Cys 
1               5                   10 

 
           
             209  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            209 

Cys Leu Gln Cys Met Gly Cys Cys Phe Ser Arg Ala 
1               5                   10 

 
           
             210  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            210 

Gln Cys Met Gly Cys Cys Phe Ser Arg Ala Tyr Cys 
1               5                   10 

 
           
             211  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            211 

Cys Cys Met Gly Cys Cys Phe Ser Arg Ala Tyr Pro 
1               5                   10 

 
           
             212  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            212 

Met Gly Cys Cys Phe Ser Arg Ala Tyr Pro Thr Cys 
1               5                   10 

 
           
             213  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            213 

Cys Gly Cys Cys Phe Ser Arg Ala Tyr Pro Thr Pro 
1               5                   10 

 
           
             214  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            214 

Cys Cys Phe Ser Arg Ala Tyr Pro Thr Pro Leu Cys 
1               5                   10 

 
           
             215  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            215 

Phe Ser Arg Ala Tyr Pro Thr Pro Leu Arg Ser Cys 
1               5                   10 

 
           
             216  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            216 

Cys Ser Arg Ala Tyr Pro Thr Pro Leu Arg Ser Lys 
1               5                   10 

 
           
             217  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            217 

Arg Ala Tyr Pro Thr Pro Leu Arg Ser Lys Lys Cys 
1               5                   10 

 
           
             218  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            218 

Cys Ala Tyr Pro Thr Pro Leu Arg Ser Lys Lys Thr 
1               5                   10 

 
           
             219  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            219 

Tyr Pro Thr Pro Leu Arg Ser Lys Lys Thr Met Cys 
1               5                   10 

 
           
             220  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            220 

Cys Pro Thr Pro Leu Arg Ser Lys Lys Thr Met Leu 
1               5                   10 

 
           
             221  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            221 

Thr Pro Leu Arg Ser Lys Lys Thr Met Leu Val Cys 
1               5                   10 

 
           
             222  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            222 

Cys Pro Leu Arg Ser Lys Lys Thr Met Leu Val Gln 
1               5                   10 

 
           
             223  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            223 

Leu Arg Ser Lys Lys Thr Met Leu Val Gln Lys Cys 
1               5                   10 

 
           
             224  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            224 

Cys Arg Ser Lys Lys Thr Met Leu Val Gln Lys Asn 
1               5                   10 

 
           
             225  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            225 

Ser Lys Lys Thr Met Leu Val Gln Lys Asn Val Cys 
1               5                   10 

 
           
             226  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            226 

Cys Lys Lys Thr Met Leu Val Gln Lys Asn Val Thr 
1               5                   10 

 
           
             227  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            227 

Lys Thr Met Leu Val Gln Lys Asn Val Thr Ser Cys 
1               5                   10 

 
           
             228  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            228 

Cys Thr Met Leu Val Gln Lys Asn Val Thr Ser Glu 
1               5                   10 

 
           
             229  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            229 

Met Leu Val Gln Lys Asn Val Thr Ser Glu Ser Cys 
1               5                   10 

 
           
             230  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            230 

Cys Leu Val Gln Lys Asn Val Thr Ser Glu Ser Thr 
1               5                   10 

 
           
             231  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            231 

Cys Gln Lys Asn Val Thr Ser Glu Ser Thr Cys Cys 
1               5                   10 

 
           
             232  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            232 

Lys Asn Val Thr Ser Glu Ser Thr Cys Cys Val Cys 
1               5                   10 

 
           
             233  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            233 

Cys Asn Val Thr Ser Glu Ser Thr Cys Cys Val Ala 
1               5                   10 

 
           
             234  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            234 

Val Thr Ser Glu Ser Thr Cys Cys Val Ala Lys Cys 
1               5                   10 

 
           
             235  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            235 

Cys Thr Ser Glu Ser Thr Cys Cys Val Ala Lys Ser 
1               5                   10 

 
           
             236  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            236 

Ser Glu Ser Thr Cys Cys Val Ala Lys Ser Tyr Cys 
1               5                   10 

 
           
             237  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            237 

Cys Glu Ser Thr Cys Cys Val Ala Lys Ser Tyr Asn 
1               5                   10 

 
           
             238  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            238 

Ser Thr Cys Cys Val Ala Lys Ser Tyr Asn Arg Cys 
1               5                   10 

 
           
             239  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            239 

Cys Thr Cys Cys Val Ala Lys Ser Tyr Asn Arg Val 
1               5                   10 

 
           
             240  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            240 

Cys Cys Val Ala Lys Ser Tyr Asn Arg Val Thr Cys 
1               5                   10 

 
           
             241  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            241 

Val Ala Lys Ser Tyr Asn Arg Val Thr Val Met Cys 
1               5                   10 

 
           
             242  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            242 

Cys Ala Lys Ser Tyr Asn Arg Val Thr Val Met Gly 
1               5                   10 

 
           
             243  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            243 

Lys Ser Tyr Asn Arg Val Thr Val Met Gly Gly Cys 
1               5                   10 

 
           
             244  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            244 

Cys Ser Tyr Asn Arg Val Thr Val Met Gly Gly Phe 
1               5                   10 

 
           
             245  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            245 

Tyr Asn Arg Val Thr Val Met Gly Gly Phe Lys Cys 
1               5                   10 

 
           
             246  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            246 

Cys Asn Arg Val Thr Val Met Gly Gly Phe Lys Val 
1               5                   10 

 
           
             247  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            247 

Arg Val Thr Val Met Gly Gly Phe Lys Val Glu Cys 
1               5                   10 

 
           
             248  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            248 

Cys Val Thr Val Met Gly Gly Phe Lys Val Glu Asn 
1               5                   10 

 
           
             249  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            249 

Thr Val Met Gly Gly Phe Lys Val Glu Asn His Cys 
1               5                   10 

 
           
             250  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            250 

Cys Val Met Gly Gly Phe Lys Val Glu Asn His Thr 
1               5                   10 

 
           
             251  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            251 

Cys Gly Gly Phe Lys Val Glu Asn His Thr Ala Cys 
1               5                   10 

 
           
             252  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            252 

Cys Phe Lys Val Glu Asn His Thr Ala Cys His Cys 
1               5                   10 

 
           
             253  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            253 

Lys Val Glu Asn His Thr Ala Cys His Cys Ser Cys 
1               5                   10 

 
           
             254  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            254 

Cys Val Glu Asn His Thr Ala Cys His Cys Ser Thr 
1               5                   10 

 
           
             255  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            255 

Glu Asn His Thr Ala Cys His Cys Ser Thr Cys Cys 
1               5                   10 

 
           
             256  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            256 

Cys Asn His Thr Ala Cys His Cys Ser Thr Cys Tyr 
1               5                   10 

 
           
             257  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            257 

His Thr Ala Cys His Cys Ser Thr Cys Tyr Tyr Cys 
1               5                   10 

 
           
             258  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            258 

Cys Thr Ala Cys His Cys Ser Thr Cys Tyr Tyr His 
1               5                   10 

 
           
             259  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            259 

Ala Cys His Cys Ser Thr Cys Tyr Tyr His Lys Cys 
1               5                   10 

 
           
             260  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            260 

Cys Cys His Cys Ser Thr Cys Tyr Tyr His Lys Ser 
1               5                   10 

 
           
             261  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                          to the C or N terminal  
             
           
            261 

Asn Ser Cys Glu Leu Thr Asn Ile Thr Ile Ala Cys 
1               5                   10 

 
           
             262  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            262 

Cys Ser Cys Glu Leu Thr Asn Ile Thr Ile Ala Ile 
1               5                   10 

 
           
             263  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            263 

Cys Glu Leu Thr Asn Ile Thr Ile Ala Ile Glu Cys 
1               5                   10 

 
           
             264  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            264 

Leu Thr Asn Ile Thr Ile Ala Ile Glu Lys Glu Cys 
1               5                   10 

 
           
             265  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            265 

Cys Thr Asn Ile Thr Ile Ala Ile Glu Lys Glu Glu 
1               5                   10 

 
           
             266  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            266 

Asn Ile Thr Ile Ala Ile Glu Lys Glu Glu Cys Cys 
1               5                   10 

 
           
             267  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            267 

Cys Ile Thr Ile Ala Ile Glu Lys Glu Glu Cys Arg 
1               5                   10 

 
           
             268  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            268 

Cys Ile Ala Ile Glu Lys Glu Glu Cys Arg Phe Cys 
1               5                   10 

 
           
             269  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            269 

Ala Ile Glu Lys Glu Glu Cys Arg Phe Cys Ile Cys 
1               5                   10 

 
           
             270  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            270 

Cys Ile Glu Lys Glu Glu Cys Arg Phe Cys Ile Ser 
1               5                   10 

 
           
             271  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            271 

Glu Lys Glu Glu Cys Arg Phe Cys Ile Ser Ile Cys 
1               5                   10 

 
           
             272  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            272 

Cys Lys Glu Glu Cys Arg Phe Cys Ile Ser Ile Asn 
1               5                   10 

 
           
             273  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            273 

Glu Glu Cys Arg Phe Cys Ile Ser Ile Asn Thr Cys 
1               5                   10 

 
           
             274  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            274 

Cys Glu Cys Arg Phe Cys Ile Ser Ile Asn Thr Thr 
1               5                   10 

 
           
             275  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            275 

Phe Cys Ile Ser Ile Asn Thr Thr Trp Cys Ala Cys 
1               5                   10 

 
           
             276  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            276 

Cys Cys Ile Ser Ile Asn Thr Thr Trp Cys Ala Gly 
1               5                   10 

 
           
             277  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            277 

Cys Ser Ile Asn Thr Thr Trp Cys Ala Gly Tyr Cys 
1               5                   10 

 
           
             278  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            278 

Ile Asn Thr Thr Trp Cys Ala Gly Tyr Cys Tyr Cys 
1               5                   10 

 
           
             279  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            279 

Cys Asn Thr Thr Trp Cys Ala Gly Tyr Cys Tyr Thr 
1               5                   10 

 
           
             280  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            280 

Thr Thr Trp Cys Ala Gly Tyr Cys Tyr Thr Arg Cys 
1               5                   10 

 
           
             281  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            281 

Cys Thr Trp Cys Ala Gly Tyr Cys Tyr Thr Arg Asp 
1               5                   10 

 
           
             282  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            282 

Trp Cys Ala Gly Tyr Cys Tyr Thr Arg Asp Leu Cys 
1               5                   10 

 
           
             283  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            283 

Cys Cys Ala Gly Tyr Cys Tyr Thr Arg Asp Leu Val 
1               5                   10 

 
           
             284  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            284 

Ala Gly Tyr Cys Tyr Thr Arg Asp Leu Val Tyr Cys 
1               5                   10 

 
           
             285  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            285 

Cys Gly Tyr Cys Tyr Thr Arg Asp Leu Val Tyr Lys 
1               5                   10 

 
           
             286  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            286 

Tyr Cys Tyr Thr Arg Asp Leu Val Tyr Lys Asp Cys 
1               5                   10 

 
           
             287  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            287 

Cys Cys Tyr Thr Arg Asp Leu Val Tyr Lys Asp Pro 
1               5                   10 

 
           
             288  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            288 

Tyr Thr Arg Asp Leu Val Tyr Lys Asp Pro Ala Cys 
1               5                   10 

 
           
             289  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            289 

Cys Thr Arg Asp Leu Val Tyr Lys Asp Pro Ala Arg 
1               5                   10 

 
           
             290  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            290 

Arg Asp Leu Val Tyr Lys Asp Pro Ala Arg Pro Cys 
1               5                   10 

 
           
             291  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            291 

Cys Asp Leu Val Tyr Lys Asp Pro Ala Arg Pro Lys 
1               5                   10 

 
           
             292  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            292 

Leu Val Tyr Lys Asp Pro Ala Arg Pro Lys Ile Cys 
1               5                   10 

 
           
             293  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            293 

Cys Val Tyr Lys Asp Pro Ala Arg Pro Lys Ile Gln 
1               5                   10 

 
           
             294  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            294 

Tyr Lys Asp Pro Ala Arg Pro Lys Ile Gln Lys Cys 
1               5                   10 

 
           
             295  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            295 

Cys Lys Asp Pro Ala Arg Pro Lys Ile Gln Lys Thr 
1               5                   10 

 
           
             296  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            296 

Asp Pro Ala Arg Pro Lys Ile Gln Lys Thr Cys Cys 
1               5                   10 

 
           
             297  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            297 

Cys Pro Ala Arg Pro Lys Ile Gln Lys Thr Cys Thr 
1               5                   10 

 
           
             298  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            298 

Ala Arg Pro Lys Ile Gln Lys Thr Cys Thr Phe Cys 
1               5                   10 

 
           
             299  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            299 

Cys Arg Pro Lys Ile Gln Lys Thr Cys Thr Phe Lys 
1               5                   10 

 
           
             300  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            300 

Pro Lys Ile Gln Lys Thr Cys Thr Phe Lys Glu Cys 
1               5                   10 

 
           
             301  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            301 

Cys Lys Ile Gln Lys Thr Cys Thr Phe Lys Glu Leu 
1               5                   10 

 
           
             302  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            302 

Ile Gln Lys Thr Cys Thr Phe Lys Glu Leu Val Cys 
1               5                   10 

 
           
             303  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            303 

Cys Gln Lys Thr Cys Thr Phe Lys Glu Leu Val Tyr 
1               5                   10 

 
           
             304  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            304 

Lys Thr Cys Thr Phe Lys Glu Leu Val Tyr Glu Cys 
1               5                   10 

 
           
             305  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            305 

Cys Thr Cys Thr Phe Lys Glu Leu Val Tyr Glu Thr 
1               5                   10 

 
           
             306  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            306 

Cys Thr Phe Lys Glu Leu Val Tyr Glu Thr Val Cys 
1               5                   10 

 
           
             307  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            307 

Phe Lys Glu Leu Val Tyr Glu Thr Val Arg Val Cys 
1               5                   10 

 
           
             308  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            308 

Cys Lys Glu Leu Val Tyr Glu Thr Val Arg Val Pro 
1               5                   10 

 
           
             309  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            309 

Cys Leu Val Tyr Glu Thr Val Arg Val Pro Gly Cys 
1               5                   10 

 
           
             310  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            310 

Val Tyr Glu Thr Val Arg Val Pro Gly Cys Ala Cys 
1               5                   10 

 
           
             311  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            311 

Cys Tyr Glu Thr Val Arg Val Pro Gly Cys Ala His 
1               5                   10 

 
           
             312  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            312 

Glu Thr Val Arg Val Pro Gly Cys Ala His His Cys 
1               5                   10 

 
           
             313  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            313 

Cys Thr Val Arg Val Pro Gly Cys Ala His His Ala 
1               5                   10 

 
           
             314  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            314 

Val Arg Val Pro Gly Cys Ala His His Ala Asp Cys 
1               5                   10 

 
           
             315  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            315 

Cys Arg Val Pro Gly Cys Ala His His Ala Asp Ser 
1               5                   10 

 
           
             316  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            316 

Val Pro Gly Cys Ala His His Ala Asp Ser Leu Cys 
1               5                   10 

 
           
             317  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            317 

Cys Pro Gly Cys Ala His His Ala Asp Ser Leu Tyr 
1               5                   10 

 
           
             318  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            318 

Gly Cys Ala His His Ala Asp Ser Leu Tyr Thr Cys 
1               5                   10 

 
           
             319  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            319 

Cys Cys Ala His His Ala Asp Ser Leu Tyr Thr Tyr 
1               5                   10 

 
           
             320  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            320 

Ala His His Ala Asp Ser Leu Tyr Thr Tyr Pro Cys 
1               5                   10 

 
           
             321  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            321 

Cys His His Ala Asp Ser Leu Tyr Thr Tyr Pro Val 
1               5                   10 

 
           
             322  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            322 

His Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala Cys 
1               5                   10 

 
           
             323  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            323 

Cys Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala Thr 
1               5                   10 

 
           
             324  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            324 

Cys Ser Leu Tyr Thr Tyr Pro Val Ala Thr Gln Cys 
1               5                   10 

 
           
             325  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            325 

Cys Tyr Thr Tyr Pro Val Ala Thr Gln Cys His Cys 
1               5                   10 

 
           
             326  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            326 

Thr Tyr Pro Val Ala Thr Gln Cys His Cys Gly Cys 
1               5                   10 

 
           
             327  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            327 

Cys Tyr Pro Val Ala Thr Gln Cys His Cys Gly Lys 
1               5                   10 

 
           
             328  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            328 

Pro Val Ala Thr Gln Cys His Cys Gly Lys Cys Cys 
1               5                   10 

 
           
             329  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            329 

Cys Val Ala Thr Gln Cys His Cys Gly Lys Cys Asp 
1               5                   10 

 
           
             330  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            330 

Ala Thr Gln Cys His Cys Gly Lys Cys Asp Ser Cys 
1               5                   10 

 
           
             331  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            331 

Cys Thr Gln Cys His Cys Gly Lys Cys Asp Ser Asp 
1               5                   10 

 
           
             332  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            332 

Gln Cys His Cys Gly Lys Cys Asp Ser Asp Ser Cys 
1               5                   10 

 
           
             333  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            333 

Cys Cys His Cys Gly Lys Cys Asp Ser Asp Ser Thr 
1               5                   10 

 
           
             334  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            334 

Cys Cys Gly Lys Cys Asp Ser Asp Ser Thr Asp Cys 
1               5                   10 

 
           
             335  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            335 

Gly Lys Cys Asp Ser Asp Ser Thr Asp Cys Thr Cys 
1               5                   10 

 
           
             336  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            336 

Cys Lys Cys Asp Ser Asp Ser Thr Asp Cys Thr Val 
1               5                   10 

 
           
             337  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            337 

Cys Asp Ser Asp Ser Thr Asp Cys Thr Val Arg Cys 
1               5                   10 

 
           
             338  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            338 

Ser Asp Ser Thr Asp Cys Thr Val Arg Gly Leu Cys 
1               5                   10 

 
           
             339  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            339 

Cys Asp Ser Thr Asp Cys Thr Val Arg Gly Leu Gly 
1               5                   10 

 
           
             340  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            340 

Ser Thr Asp Cys Thr Val Arg Gly Leu Gly Pro Cys 
1               5                   10 

 
           
             341  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            341 

Cys Thr Asp Cys Thr Val Arg Gly Leu Gly Pro Ser 
1               5                   10 

 
           
             342  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            342 

Cys Cys Thr Val Arg Gly Leu Gly Pro Ser Tyr Cys 
1               5                   10 

 
           
             343  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            343 

Thr Val Arg Gly Leu Gly Pro Ser Tyr Cys Ser Cys 
1               5                   10 

 
           
             344  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            344 

Cys Val Arg Gly Leu Gly Pro Ser Tyr Cys Ser Phe 
1               5                   10 

 
           
             345  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            345 

Arg Gly Leu Gly Pro Ser Tyr Cys Ser Phe Gly Cys 
1               5                   10 

 
           
             346  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            346 

Cys Gly Leu Gly Pro Ser Tyr Cys Ser Phe Gly Glu 
1               5                   10 

 
           
             347  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            347 

Leu Gly Pro Ser Tyr Cys Ser Phe Gly Glu Met Cys 
1               5                   10 

 
           
             348  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            348 

Cys Gly Pro Ser Tyr Cys Ser Phe Gly Glu Met Lys 
1               5                   10 

 
           
             349  
             12  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            349 

Pro Ser Tyr Cys Ser Phe Gly Glu Met Lys Glu Cys 
1               5                   10 

 
           
             350  
             25  
             PRT  
             Artificial  
             
               Concatonated sequences of hFSH  
             
           
            350 

Val Tyr Glu Thr Val Arg Val Pro Gly Cys Ala Cys Xaa Ala Asp Ser 
1               5                   10                  15 
Leu Tyr Thr Tyr Pro Val Ala Thr Gln 
            20                  25 

 
           
             351  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            351 

Lys Thr Ala Thr Phe Lys Glu Leu Val Tyr Glu Thr Cys 
1               5                   10 

 
           
             352  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            352 

Cys Thr Ala Thr Phe Lys Glu Leu Val Tyr Glu Thr Val 
1               5                   10 

 
           
             353  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            353 

Ala Thr Phe Lys Glu Leu Val Tyr Glu Thr Val Arg Cys 
1               5                   10 

 
           
             354  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            354 

Cys Thr Phe Lys Glu Leu Val Tyr Glu Thr Val Arg Val 
1               5                   10 

 
           
             355  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            355 

Phe Lys Glu Leu Val Tyr Glu Thr Val Arg Val Pro Cys 
1               5                   10 

 
           
             356  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            356 

Cys Lys Glu Leu Val Tyr Glu Thr Val Arg Val Pro Gly 
1               5                   10 

 
           
             357  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            357 

Glu Leu Val Tyr Glu Thr Val Arg Val Pro Gly Ala Cys 
1               5                   10 

 
           
             358  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            358 

Cys Leu Val Tyr Glu Thr Val Arg Val Pro Gly Ala Ala 
1               5                   10 

 
           
             359  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            359 

Val Tyr Glu Thr Val Arg Val Pro Gly Ala Ala His Cys 
1               5                   10 

 
           
             360  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            360 

Cys Tyr Glu Thr Val Arg Val Pro Gly Ala Ala His His 
1               5                   10 

 
           
             361  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            361 

Glu Thr Val Arg Val Pro Gly Ala Ala His His Ala Cys 
1               5                   10 

 
           
             362  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            362 

Cys Thr Val Arg Val Pro Gly Ala Ala His His Ala Asp 
1               5                   10 

 
           
             363  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            363 

Val Arg Val Pro Gly Ala Ala His His Ala Asp Ser Cys 
1               5                   10 

 
           
             364  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            364 

Cys Arg Val Pro Gly Ala Ala His His Ala Asp Ser Leu 
1               5                   10 

 
           
             365  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            365 

Val Pro Gly Ala Ala His His Ala Asp Ser Leu Tyr Cys 
1               5                   10 

 
           
             366  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            366 

Cys Pro Gly Ala Ala His His Ala Asp Ser Leu Tyr Thr 
1               5                   10 

 
           
             367  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            367 

Gly Ala Ala His His Ala Asp Ser Leu Tyr Thr Tyr Cys 
1               5                   10 

 
           
             368  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            368 

Cys Ala Ala His His Ala Asp Ser Leu Tyr Thr Tyr Pro 
1               5                   10 

 
           
             369  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            369 

Ala His His Ala Asp Ser Leu Tyr Thr Tyr Pro Val Cys 
1               5                   10 

 
           
             370  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            370 

Cys His His Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala 
1               5                   10 

 
           
             371  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            371 

His Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala Thr Cys 
1               5                   10 

 
           
             372  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            372 

Cys Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala Thr Gln 
1               5                   10 

 
           
             373  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            373 

Asp Ser Leu Tyr Thr Tyr Pro Val Ala Thr Gln Ala Cys 
1               5                   10 

 
           
             374  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            374 

Cys Ser Leu Tyr Thr Tyr Pro Val Ala Thr Gln Ala His 
1               5                   10 

 
           
             375  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            375 

Leu Tyr Thr Tyr Pro Val Ala Thr Gln Ala His Ala Cys 
1               5                   10 

 
           
             376  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            376 

Cys Tyr Thr Tyr Pro Val Ala Thr Gln Ala His Ala Gly 
1               5                   10 

 
           
             377  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            377 

Thr Tyr Pro Val Ala Thr Gln Ala His Ala Gly Lys Cys 
1               5                   10 

 
           
             378  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            378 

Cys Tyr Pro Val Ala Thr Gln Ala His Ala Gly Lys Ala 
1               5                   10 

 
           
             379  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            379 

Pro Val Ala Thr Gln Ala His Ala Gly Lys Ala Asp Cys 
1               5                   10 

 
           
             380  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            380 

Cys Val Ala Thr Gln Ala His Ala Gly Lys Ala Asp Ser 
1               5                   10 

 
           
             381  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            381 

Ala Thr Gln Ala His Ala Gly Lys Ala Asp Ser Asp Cys 
1               5                   10 

 
           
             382  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            382 

Cys Thr Gln Ala His Ala Gly Lys Ala Asp Ser Asp Ser 
1               5                   10 

 
           
             383  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            383 

Gln Ala His Ala Gly Lys Ala Asp Ser Asp Ser Thr Cys 
1               5                   10 

 
           
             384  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            384 

Cys Ala His Ala Gly Lys Ala Asp Ser Asp Ser Thr Asp 
1               5                   10 

 
           
             385  
             13  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            385 

Ala Asp Ser Leu Tyr Thr Tyr Pro Val Ala Thr Gln Cys 
1               5                   10 

 
           
             386  
             10  
             PRT  
             Artificial  
             
               Fragment of hFSH with Cys attached 
                         to the C or N terminal  
             
           
            386 

Val Tyr Glu Thr Val Arg Val Pro Gly Cys 
1               5                   10