Abstract:
Disclosed are the methods for producing recombinant viruses using site-specific recombination in vitro. In the present invention, circular viral genomic DNAs are digested with restriction enzymes to generate a linear form viral genomic DNAs flanked by site-specific recombination sites, and then are subjected to site-specific recombination with the desired genomic materials flanked by site-specific recombination sites in vitro. According to the present invention, since the site-specific recombination mixture can be applied to host cells without further procedures of selecting the desired recombinant viral genomic DNAs, it is possible to obtain numerous recombinant viruses rapidly at the same time. Thus, the present invention can be used as a high throughput system for generating and screening hundreds or thousands of recombinant viruses.

Description:
BACKGROUND OF THE INVENTION  
         [0001]    1. Field of the Invention  
           [0002]    This invention relates to a method for producing recombinant viruses and vectors for the same. Furthermore, this invention relates to DNA constructs for the method and the recombinant viruses produced by the method.  
           [0003]    2. Description of the Related Art  
           [0004]    A recombinant viral vector means a genetically engineered vector, which is used in, for example: the preparation of a vaccine; analysis of function of a gene, a group of genes, or a genomic domain; production of proteins; and gene therapy. Genomic materials, which could be integrated into a viral vector, include any genomic materials such as a gene, cDNA, genomic DNA, DNA sequences encoding peptides or proteins, RNA, anti-sense RNA, siRNA, DNA for si RNA, promoter, enhancer etc. A virus, which is used in the preparation of a vector, includes baculovirus, adenovirus, adeno-associated virus (AAV), retrovirus, Herpes virus, hepatitis B virus (HBV), polioma virus, sindbid virus, and vacxnia virus etc.  
           [0005]    Retrovirus is known as one of the most widely used viruses in gene therapy [Clive Patience et al. J Virol, 72:2671-2676, 1998, Marshall, Science, 269: 1050-1055,1995]. Retroviral genome has two LTRs, capsid sequences and 3 (three) coding regions (gag, pol and env), and the method of its preparation and its use in vitro and in vivo are disclosed (see WO9908692A1 and EP 453242). The clinical application of retroviral vector, however, has the following disadvantages: it forms a replication-competent retrovirus (RCR) (Sharon K. Powell et al. J. Virol., 73:8813-8816, 1999); it has relatively lower level of gene expression, and the level decreases continuously in vivo; and the titer of virus is low. Furthermore, the retrovirus cannot transfer a gene to a non-dividing cell [Jaffe, E. M. Cancer Res., 53:2221-2226, 1993; Bender, M. A. et al. J. Virol., 61:1639-1646, 1987].  
           [0006]    Adenovirus has a linear genome (sized about 36 kb) and includes replication origin and capsid signals near to the left inverse terminal region (ITR) (103 bp) [Shenk, Adenoviridae: The Viruses and Their Replication. In: Fields B N, Knipe D M Howley P M, ed. Virology. Philadelphia: Raven publishers, 1996: 2111-2148]. Usually, adenoviral vectors have been made by deleting E1 site of the viral genome, which is essential for the viral replication. The viruses including foreign genomic materials substituting E1 site, are transferred to the packaging cells that provide E1 proteins. Thereby, the viral replication occurs only in the packaging cells. Representative packaging cells include, for example, HEK293 cell, 911 cell, and PER. C6 cell, etc. [Hitt M M et al., Advances in Pharmacology, vol. 40, 137-206 (1997), Wang Y. and Huang S., Drug]. Adenoviral vector has advantages such as safty, affinity to various cells, possibility of infecting dividing cells and higher titer (10 11  pfu/ml). Thus, adenovirus has been used in the preparation of a vector expressing heterologous genes.  
           [0007]    Adeno-associated virus (sized about 4700 bp) has ITRs (sized about 145 bp) as replication origins at each terminus. It can be integrated into the genomic DNAs of various types of host cells safely and specifically.  
           [0008]    In order to use a recombinant virus as a vector, it should be modified not to replicate themselves in the infected cells. Therefore, defective viruses, which have deletions of some essential regions of genome for viral replication, are usually employed in the preparation of the recombinant viral vectors. As for a retrovirus, for example, gag, pol and/or env genes are deleted, and the regions are replaced with desired genomic materials [Bender et al., J. Virol. 61 (1987) 1639]. Regarding an adenovirus, E1, E2 and/or E4 sites are deleted for the preparation of a viral vector [Levrero et al., Gene 101 (1991) 195; Gosh-Choudhury et al., Gene 50 (1986) 161, Van der Vliet et al., (1975)]. On the other hand, a pseudo-virus vector, which consists of only the essential regions (such as, ITR and capsid sequences) of the replication, has been used also for obtaining recombinant viruses (see WO95/02697).  
           [0009]    In addition, baculoviruses, which are known as having circular genomic DNAs, have been used for the preparation of viral vectors. Baculoviridae are infectious on invertebrates, such as insects and crustacea. AcNPV is one of the most widely used baculoviruses, and it contains double strand circular genomic DNAs (sized about 134 kb) (GenBank: NC — 001623). Smith et al. developed recombinant baculoviruses by inserting β-interferon genes, and successfully obtained interferon proteins from insect cells using the recombinant baculoviruses (Smith G E., Mol. Cell. Biol., V3, p2156-2165, 1983). Since then, numerous genes were expressed and produced using recombinant baculoviral system. The features of the methods are that: i) it is possible to produce desired recombinant baculoviruses at high efficiency by employing polyhedrin promoter; and ii) it can be used for the expression of genes that do not exhibit their activities when incubated in bacteria. That is because post-translational modification is carried out in insect cells. However, the size of genome of baculovirus made it difficult to engineer the genome through conventional restriction and ligation method. Therefore, homologous recombination has been used in insect cells generally. In order for the homologous recombination, carrier vectors containing desired genes and nucleic acid sequences necessary for the homologous recombination are prepared. Then, insect cells are transfected with the carrier vectors and viral genomic DNAs to induce recombination. In that method, however, since the production efficiency of recombinant viruses is relatively low (0.1-2%), repetitive screening of baculoviral plaques should be performed in order to obtain the desired recombinant viruses. Thus, it is also considered as being an inefficient method.  
           [0010]    Kitts P A et al. disclosed an improved method that increased production efficiency of recombinant viruses (Nucleic Acids Res., October 1990; 18: 5667-5672. Kitts P A et al). They made baculoviral genomic DNAs to be in linear form using restriction enzyme. In Kitts&#39; method, a linker, which includes Bsu36I recognition base sequences, CCTNAGG, or lacZ genes, is inserted to wild-type  Autographa californica  nuclear polyhedrosis virus (AcNPV) genome at polyherin locus in order to introduce Bsu36I site recognition nucleic acid sequences. Next, the viral DNAs are digested with Bsu36I restriction enzyme, and then transferred to insect cells together with carrier vectors containing desired genomic materials so as to induce homologous recombination. The production efficiency of the desired recombinant viruses is from 26% to 44%. According to that method, linear digested DNA segments, which are not subjected to recombination, are excluded selectively from the production of viral vectors. Thus, the desired recombinant viruses expressing desired protein could be generated with relatively high efficiency. However, the method has disadvantages as follows: i) it is not possible to digest Bsu36I recognition sites up to 100% and ii) since the digested DNA segments are fused with each other in insect cells by ligase, selection procedures for the desired viral vectors need be employed.  
           [0011]    Peakman T C et al. provided another method using site-specific recombination of cre/loxP system in vitro (Peakman T C et al., Nucleic Acids Research, Vol 20, Issue 3 495-500). Briefly, they prepared baculoviruses containing loxP sites and applied them to site-specific recombination in vitro with desired genomic materials by reacting the viruses with transfer vectors having the genomic materials flanked with loxP sites, in the presence of cre recombination enzyme. In that method, time-period for producing recombinant viruses is shortened. However, the production efficiency of desired recombinant viruses is still low (0.2% to 49%), thereby it is not suitable to be used as a high-throughput system for the preparation of recombinant viruses.  
           [0012]    In addition, Luckow V A et al. reported a method of inserting desired genomic cassette to baculoviral genome by site-specific recombination in a cell (Luckow V A et al., Virol., August 1993; 67: 4566-4579). According to Luckow&#39;s method, firstly, a shuttle vector (sized about 130 kb) is prepared by inserting plasmid replication origin, which replicates in bacteria, kanamycin resistance gene and attTn7 recombination sequences into baculoviral genome. The shuttle was named “Bacmid”. DH10Bac was generated by transforming  E. coli  with Bacmid and helper plasmid, which in turn was transformed using a plasmid containing desired genes and polyhedrin promoter to prepare recombinant Bacmid in  E coli . The resulting recombinant Bacmid is isolated and transferred to insect cells to obtain recombinant viruses. According to that method, baculoviruses could be obtained with relatively high efficiency by using site-specific recombination of transposon. That method, however, required complex procedures, for example, cloning desired genomic materials to Tn7 Bacmid carrier vectors; transforming DH10Bac for the recombination of Tn7 baculoviruses; selecting and isolating bacteria having Bacmid that contains desired genomic materials; purifying Bacmid DNAs (sized about 130 kb) from the isolated bacteria; and transferring the DNAs again to insect cells to produce recombinant virues.  
           [0013]    Thus, non of the above-mentioned methods of the prior arts have provided suitable ways to overcome the problems of relatively long time-period in preparing recombinant viruses (about 3 to 6 weeks) and low production efficiency. Therefore, there have been demands for the development of an improved method  
           [0014]    In this regard, we have carried out studies to produce recombinant viruses having desired genomic materials more rapidly and efficiently, thereby we have completed this invention by preparing recombinant viral vectors in vitro using site-specific recombination and employing the vectors to produce recombinant viruses. Since the viral vectors obtained in vitro according to this invention can be applied to animal cells directly without further procedures like selection, the present invention not only simplifies the total procedures significantly but also provides desired recombinant viruses with up to 100% production efficiency. Thus, higher virus titer can be obtained according to the present invention.  
         SUMMARY OF THE INVENTION  
         [0015]    Any publications referenced herein are hereby incorporated by reference in this application in order to more fully describe the state of the art to which the present invention pertains.  
           [0016]    It is important to understand the present invention to note that all technical and scientific terms used herein, unless otherwise defined, are intended to have the same meaning as commonly understood by one of ordinary skill in the art. The techniques used herein are also those that are known to one of ordinary skill in the art, unless stated otherwise.  
           [0017]    Reference to particular buffers, media, reagents, cells, culture conditions and the like, or to some subclass of same, is not intended to be limiting, but should be read to include all such related materials that one of ordinary skill in the art would recognize as being of interest or value in the particular context in which that discussion is presented. For example, it is often possible to substitute one buffer system or culture medium for another, such that a different but known way is used to achieve the same goals as those to which the use of suggested method, material or composition is directed.  
           [0018]    It is an object of this invention to provide more rapid and efficient method for producing recombinant viruses.  
           [0019]    In order to accomplish this object, this invention employs recombinant viral vectors that are prepared in vitro by site-specific recombination.  
           [0020]    In the present invention, viral genomic DNAs are made in linear form by restriction enzymes before being applied to site-specific recombination in vitro. Since the linear viral genomic DNAs, which are not subjected to the site-specific recombination, cannot replicate by themselves, almost all of the expressed viruses are believed to be those from the circular viral genomic DNAs. Thus, according to the present invention, it is possible to obtain recombinant viruses at high efficiency without the procedures for selecting and isolating the desired recombinant viruses. Accordingly, the method of the present invention can be applied to a virus having circular genomic DNAs, for example, polioma virus, papiloma virus and hepatitis B virus, etc.  
           [0021]    Referring to site-specific recombination, it is an event occurring naturally in various living organisms. Enzymes for the site-specific recombination contain not only endonuclease activity but also ligase activity, so they recognize a certain part of DNA sequences and replace it with any other corresponding DNA sequences [Yang W. and Mizuuchi K., Structure, 1997, Vol. 5, 1401-1406(9)]. Int/att system from bacterio λ phage, Cre/LoxP system from P1 bacterio phage and FLP-FRT system from yeast are well developed site-specific recombination systems.  
           [0022]    Site-specific recombination system has been used in i) expression control of desired genomic materials cloned into recombinant viruses, ii) excising helper-virus when preparing helper-viral dependent viruses, iii) increasing the efficiency of homologous recombination in packaging cells, or iv) inducing site-specific recombination between carrier vectors and viral genome in packaging cells to prepare recombinant viruses [Philip N G et al., Biotechniques, vol. 29. 524-528 (2000), Philip N G et al., Human Gene therapy, vol. 10., 2667-2672 (1999), Hardy S et al., Journal of Viology, vol. 71., 1842-1849 (1997), Parks R J et al., Proc. Natl. Acad. Sci USA, vol 93., 13565-13570 (1996)]. However, the said applications of the site-specific recombination disclosed in the prior arts were merely indirect and supplementary uses in vivo or in a cell line, not in vitro.  
           [0023]    In addition, applications of site-specific recombination in the preparation of recombinant viruses having circular genomic DNAs were reported: i) in the preparation of recombinant baculoviruses in bacteria using Tn7 recombinantion, and ii) in the preparation of recombinant baculoviruses using cre/lox-P system in vitro.  
           [0024]    However, there have been no applications of site-specific recombination in vitro for specific insertion of desired genomic materials into viral genomic DNAs in order to obtain recombinant viral vectors. In particular, there have been no applications to prevent the expression of viral genomic DNAs that do not contain desired genomic materials. Thus, the present invention is characterized by employing site-specific recombination in preparing recombinant viral vectors in vitro. The present invention has unexpected advantages compared to the prior arts as follows: i) it is possible to obtain recombinant viral vectors more easily and more rapidly by employing site-specific recombinant in vitro; ii) it does not require any further procedure of selecting desired recombinant viruses from intermediate host cells, since the viral genomic DNAs are made in linear form and thereby the linear viral DNAs, which do not form in circular form by integration of the desired genomic materials, cannot be expressed; and iii) it is possible to generate the same kind of genomic materials from various types of viruses or to generate various genomic materials from the same kind of viral type muti-well (e.g. 96-well or 384-well) by employing common transfer vector containing expression cassette that does not have DNAs from viral genome.  
           [0025]    Furthermore, according to the present invention, homologous recombination in the packaging cells is not required any more. Nor, particular cell lines for inducing homologous recombination are required. Table 1 and Table 2 show the results of the comparisons between the method of the present invention (BacHTS system) and the methods of the prior arts (BacPAK6 system and BacToBac system) with regard to the processes. With the results, it is clear that the desired recombinant viruses can be obtained even more rapidly by using the method of this invention in comparison to the prior arts.  
                                                       TABLE 1                           The comparisons of the number of procedures between       the method of the present invention and       the methods of prior arts                the number of                   procedures   Days   time-period                            BacPAK6 system   11   6   10           BacToBac system   13   8   12           BacHTS system   5   3   7                      
 
           [0026]    [0026]                             TABLE 2                           The comparisons of the processes required for preparation of baculoviruses            BacPAK system   BacToBac system   BacHTS system                1. RE cut    1. LR reaction   1. Recombination        2. Agarose gel elution    2. Transformation   2. Transfection        3. Ligaion    3. Colony seeding   3. Infection        4. Transformation    4. Plasmid purification   4. Harvest and virus storage        5. Colony seeding    5. RE analysis   5. Protein expression analysis        6. Plasmid purification    6. DH10Bac ransformation        7. RE analysis    7. Streaking        8. Transfection    8. Seeding        9. Infection    9. Bacmid purification       10. Harvest and virus   10. Transfection          storage   11. Infection       11. Protein expression   12. Harvest and virus storage          analysis   13. Protein expression              analysis                    
           [0027]    Furthermore, according to the present invention, since promoter for the expression of the desired genomic materials, and fusion system and polyadenyl sequences for the preparation of fusion protein are provided from parent viral genome, the gene cassette cloned to common carrier vector could be applied to produce various types of viruses.  
           [0028]    Thus, the present invention provides significantly useful tools for studying functions of genes of which demands have been increasing greatly since the completion of genome project [Wang Y. and Huang S., Drug Discovery Today, vol. 5, 10-16 (2000)].  
           [0029]    In one embodiment, the present invention employs bacuovirus having circular genomic DNAs, and Int/att system from bacteriophage λ (Genebank: NC 001416) as a site-specific recombination system. In the Int/att system, attR site-specific recombination sites within viral genomic DNA fragment react with corresponding attL site-specific recombination sites including desired genomic materials from carrier vector, in the presence of λ integrase recombination enzyme, IHF as a co-factor and Xis, and result in vector DNA fragment containing attB and attP sites [see Hartley J L. Genome Research, 2000, Vol. 10, 1788-1795]. Other recombination systems from various organisms, for example, the Cre/loxP system from bacteriophage P1, and the FLP/FRT system from the  Saccharomyces cerevisiae  2μ circle plasmid, can be employed.  
           [0030]    When the mixture is transferred directly to suitable host cells, which are expected to express desired recombinant viruses, only the circular viral genomic DNAs containing attB1-[desired genomic materials]-attB2 can replicate and form viral particles. The linear viral DNA fragment and the vector DNAs themselves, which do not react with each other, and the circular viral DNAs having attP sites cannot replicate and cannot form viral particles. In FIG. 1, site-specific recombination between gene cassette and vBacHTS baculoviral DNAs in vitro is presented.  
           [0031]    In another embodiment, vBacHTS viral DNAs are constructed to include site-specific recombination sequences and Bsu36I recognition base sequences, which in turn are made to linear form viral DNAs flanked by 2 (two) site-specific sites with treatment of Bsu36I restriction enzyme. The linear vBacHTS DNAs cannot form viral particles since the vBacHTS viral DNAs are digested at 2 (two) Bsu36I recognition sites having nucleic acid sequences different from each other, while the circular form DNAs generated by site-specific recombination formed viral particles efficiently.  
           [0032]    In another embodiment, the reaction mixture containing viral DNAs having desired genomic materials, which are integrated by site-specific recombination, is transferred to Sf21 insect cells, and then viral packaging is detected. Thus, according to the present invention, recombinant viruses can be obtained without employing intermediate host cells, such as  E. coli  or yeast, for selecting and amplifying suitable genomic DNAs. Therefore, the recombinant viruses are suitable for the purpose of expressing desired genes from host cells. The activity measurement of GFP and GUS proteins indicates that the method of the present invention is more efficient than the method of the prior arts. 
       
    
    
     BRIEF DESCRIPTION OF THE DRAWINGS  
       [0033]    [0033]FIG. 1 illustrates gene-screening processes using BacHTS system.  
         [0034]    [0034]FIG. 2 depicts the cleavage map of pBacHTS.  
         [0035]    [0035]FIG. 3 depicts the cleavage map of pBacHTS2.  
         [0036]    [0036]FIG. 4 depicts the cleavage map of pBacPAK8_attR1.  
         [0037]    [0037]FIG. 5 depicts the cleavage map of pBacPAK_attR1R2.  
         [0038]    [0038]FIG. 6 depicts the cleavage map of pBacHTS_GFP.  
         [0039]    [0039]FIG. 7 depicts the cleavage map of pBacHTS_Flag.  
         [0040]    [0040]FIG. 8 depicts the cleavage map of pBacHTS_His.  
         [0041]    [0041]FIG. 9 depicts the cleavage map of pBacHTS_HisGst.  
         [0042]    [0042]FIG. 10 depicts the cleavage map of pBacHTS_Gst.  
         [0043]    [0043]FIG. 11 depicts the cleavage map of pBacHTS2_EGFP.  
         [0044]    [0044]FIG. 12 depicts the cleavage map of pEntr_EGFP.  
         [0045]    [0045]FIG. 13 illustrates the process of preparation of vBacHTS virus.  
         [0046]    [0046]FIG. 14 illustrates the process of preparation of bBacHTS2 clone.  
         [0047]    [0047]FIG. 15 shows the map of baculoviral polyhedrin locus.  
         [0048]    [0048]FIG. 16 illustrates agarose gel electrophoretic analysis of vBacHTS viral DNAs and bBacHTS2 viral DNAs.  
         [0049]    [0049]FIG. 17 illustrates procedures for the preparation of desired recombinant viruses using vBacHTS and gene cassette.  
         [0050]    [0050]FIG. 18 shows the results of activity assays of GFP baculoviruses and GUS recombinant viruses in vBacHTS system.  
         [0051]    [0051]FIG. 19 shows plaque assays for GFP baculoviruses and GUS recombinant viruses in vBacHTS system.  
         [0052]    [0052]FIG. 20 shows the results of activity assays of GFP baculoviruses in bBacHTS2 system.  
         [0053]    [0053]FIG. 21 shows the results of activity assays of GUS recombinant baculoviruses in bBacHTS2 system.  
         [0054]    [0054]FIG. 22 illustrates GFP image in multi-well plate and the results of western blot.  
         [0055]    [0055]FIG. 23 shows enzyme activity using X-Gluc of GUS recombinant viruses in multi-well plate.  
         [0056]    [0056]FIG. 24 shows the results of site-specific recombination in vitro.  
         [0057]    [0057]FIG. 25 depicts expression of GFP-LacZ fusion proten in Sf21 cells after 4 days from the transfer of recombinant DNAs to the cells.  
         [0058]    [0058]FIG. 26 shows the results of western blot of the expression of GFP-LacZ fusion protein using SDS-PAGE and GFP antibodies.  
         [0059]    [0059]FIG. 27 depicts LacZ enzyme activity in recombinant virus (GFP-LacZ) using X-Gal substrates.  
         [0060]    [0060]FIG. 28 shows the image of Sf21 cells infected with GFP-YPK baculoviruses from multi-well plate.  
         [0061]    [0061]FIG. 29 shows the results of screening of protein kinase (Rb, Histone H1, MBP).  
         [0062]    [0062]FIG. 30 depicts phosphorylation of Rb (retinoblastoma) protein.  
         [0063]    [0063]FIG. 31 illustrates phosphorylation of Rb protein by #2-A9 protein kinase. 
     
    
       [0064]    Preferred embodiments of this invention are described in the following examples. Other embodiments within the scope of the claims herein will be apparent to those skilled in the art from consideration of the specification or practice of the invention as disclosed herein. It is intended that the specification, together with the examples, be considered exemplary only, with the scope and the spirit of the invention being indicated by the claims that follow the example. The examples herein are meant to exemplify the various aspects of carrying out the invention and not intended to limit the scope of the invention in any way. The examples do not include detailed descriptions of conventional methods employed, such as in the performance of genomic DNA isolation, PCR, and sequencing procedures. Such methods are well known to those skilled in the art and are described in numerous publications. In addition, all the publications referred herein are integrated hereto as references.  
       DESCRIPTION OF THE PREFERRED EMBODIMENTS  
       [0065]    Preferred embodiments of this invention are described in the following examples. Other embodiments within the scope of the claims herein will be apparent to those skilled in the art from consideration of the specification or practice of the invention as disclosed herein. It is intended that the specification, together with the examples, be considered exemplary only, with the scope and the spirit of the invention being indicated by the claims which follow the examples. The examples herein are meant to exemplify the various aspects of carrying out the invention and not intended to limit the scope of the invention in any way. The examples do not include detailed descriptions of conventional methods employed, such as in the performance of genomic DNA isolation, PCR, and sequencing procedures. Such methods are well-known to those skilled in the art and are described in numerous publications. In addition, all the publications referred herein are integrated hereto as references.  
       EXAMPLES  
     Example 1  
     Preparation of pBacHTS  
       [0066]    pBacHTS viral vectors were prepared. For the preparation, firstly, pBacPAK8_R1 (see FIG. 4) was provided by cloning amplified attR1 sites and Bsu36I recognition base sequences at BamHI/EcoRI sites of a shuttle vector of pBacPAK8 (Genebank: U2446, from Clontech) and transforming DH5α (from LTI, Life Technologies Inc.) with the cloned vector, and then by incubating and screening in agar medium including ampicilin. Likewise, pBacPAK8_R1R2 was prepared by cloning PCR amplified attR2 sites and Bsu36I recognition site sequences at EcoRI/PacI site sequences of pBacPAK8_R1 (see FIG. 5). In order to obtain pBacHTS, DNA fragment (427 bp) including ccdB gene from EcoR1 digested fragments of pEntr4 plasmid (from Invitrogen) was inserted to the pBacPAK8_R1R2, and then the resulting plasmid was transferred to DB3.1 cell (from LTI). pBacHTS contained two different Bsu36I recognition sequences between attR1 site and attR2 site (FIG. 2). The attR1 sites and the attR2 sites included site-specific recombinase target sequences that reacted with attL1 and attL2 sites, respectively, in the presence of integrase from λ bacteriophage, Xis, IHF-α and IHF-β.  
       Example 2  
     Preparation of pBacHTS 6His, pBacHTS GST, pBacHTS HisGst, pBacHTS GFP and pBacHTS Flag  
       [0067]    Fusion tag was inserted to the sites in back of polyhedrin promoter in order to prepare recombinant baculovirus expressing fusion proteins. pBacHTS_Gst was designed for the preparation of expression vector for GST fusion protein. In order for this, pGEX-2T (Genbank:U13850) was PCR amplified using a primer including Bg1II and a primer including BamH1 linker, which in turn was treated with Bg1II and BamHI and then was inserted to pBacHTS/BamHI restriction sites. In addition, pBacHTS_His was designed for the preparation of a vector expressing His tag fusion protein by inserting 6 His linker to BamHI site of pBacHTS. Furthermore, pBacHTS_HisGST was designed by inserting Bg1II and BamHI treated GST genes, which were PCR amplified, to BamHI site of pBacHTS_His. On the other hand, GFP gene was PCR amplified using a primer containing BamHI linker and a primer containing KpnI linker, which in turn was digested with BamHI/KpnI. After that, the digested gene was integrated into BamHI/KpnI restriction sites of pBacHTS to generate pBacHTS_GFP. Furthermore, pBacHTS_Flag was prepared by inserting a linker expressing Flag tag at BamHI/KpnI sites of pBacHTS (see FIG. 7).  
       Example 3  
     Preparation of DNAs of vBacHTS, vBacHTS His, vBacHTS HisGst and vBacHTS GFP Viruses  
       [0068]    Baculoviruses of which polyhedrin loci were replaced by pBacHTS plasmids were prepared using homologous recombination. Briefly, Sf21 insect cells were transfected with a mixture of pBacHTS plasmid, Bsu36I digested BacPAK6 viral DNAs (from Clontech#6144-1) and Lipofectin (from LTI) to induce homologous recombination in the cells. The recombinant viruses were isolated through twice repeated plaque assays, which in turn were subjected to PCR. The purified viral clone was named vBacHTS virus (SEQ. ID No.1: the sequences of its polyhedrin locus) (see FIG. 15). As disclosed in FIG. 13, pBacHTS His (see FIG. 8), pBacHTS_HisGst (see FIG. 9), pBacHTS_GST and pBacHTS_GFP were respectively transferred together with BacPAK6 virus to generate vBacHTS_His (SEQ. ID No.2: the sequences of its polyhedrin locus), vBacHTS_HisGst (SEQ. ID No.3: the sequences of its polyhedrin locus), vBacHTS_GST (SEQ. ID No.4: the sequences of its polyhedrin locus) and vBacHTS_GFP (SEQ. ID No.5: the sequences of its polyhedrin locus) (see FIG. 15). Viral plaques were selected from agarose medium.  
       Example 4  
     Purification of Viral DNAs of vBacHTS, vBacHTS His, vBacHTS HisGst, vBacHTS GFP, and vBacHTS  
       [0069]    Viral culture medium having 1.2×10 8  pfu/ml of viral titer was produced after 3 days from infecting Sf21 cells on cell culture dish (100 mm diameter) in order to isolate vBacHTS viral DNAs. Then, 1.25×10 7  of Sf21 cells were plated again on cell culture dish (150 mm diameter), which in turn were infected with 20 times larger numbers of viruses (MOI=20). After incubating for 48 hrs, about 25 ml of cell culture medium per 1 dish was obtained. A total of 150 ml of cell culture medium from 6 dishes (150 mm diameter) was centrifugated at 20,000 rpm for 90 min, and the resulting precipitated viral particles were recovered (with Hanil, Supra22k). The recovered viral particles were suspended in 2 ml of TE (10 mM Tris-HCl pH=8.0, 1 mM EDTA), and then were treated with sucrose concentration gradient ultra-centrifuge (Beckman SW41 rotor) at 30,000 rpm. Viral particles were purified from the layer between 50% and 40% sucrose solution. The purified viruses were subjected to centrifugation again at 18,000 rpm for 90 min. After that, the precipitates of viral particles were suspended again with 2 ml of TE (10 mM Tris-HCl pH=8.0, 1 mM EDTA), and were left at 42° C. for 2 hrs after adding 0.5% SDS, 1% beta-mercaptoethanol and 0.2 mg of ProteinaseK thereto, so as to isolate viral envelopes. The viral lysate was extracted twice with the same amount of phenol/chloroform solution, and ethanol was added to the extract, thereby pure viral DNAs were obtained as precipitates. Then, 50 units of restriction enzyme Bsu361 (from New England Bio labs Inc. NEB#524) were added to the purified DNAs (10 ug). The mixture was left at 37° C. for 2 hrs before used in the following studies (see FIG. 16).  
       Example 5  
     Preparation of Gene Cassette  
       [0070]    A pEntr_GFP gene cassette was prepared by inserting PCR products of GFP between attL1 site and attL2 site. pEntr_Gus gene cassette from LTI was employed.  
       Example 6  
     Preparation of the Desired Recombinant Viruses Having GFP Gene or GUS Gene  
       [0071]    Recombinant viruses expressing GFP and Gus were generated in this study. The pEntr_GFP gene cassette (100 ng) or the pEntr_Gus gene cassette (100 ng) was transferred to a test-tube, and reacted with 100 ng of Bsu36I treated vBacHTS viral DNAs at 25° C. for 2 hrs, in the presence of integrase mixture comprising 4 ul of integrase, Xis, IHF-α and IHF-β. Then, Sf21 cells were infected with the reaction mixture together with lipofectin (from LTI), thereby recombinant viruses were generated. After 2 days, green fluorescence was observed from the insect cells including GFP gene cassette. Furthermore, after 4 days, almost every cell showed green fluorescence. Furthermore, fluorescence and symptoms of viral infection were observed in the infected cells infected.  
         [0072]    On the other hand, a mixture containing pEntr_Gus gene cassette was transferred to Sf21 cells. After 4 days, X-Gluc was added to the cells. After 2 hrs of the reaction, the Sf21 cells became blue, which supported the expression of GUS (see FIG. 18). In order to determine the productivity of the recombinant viruses, 4-day viral culture was dilated and transferred to insect cells. Next, plaques were detected 4 days after adding culture medium containing 1% low-melting point agarose to the infected cells. As the result, most plaques represented blue fluorescence (see FIG. 19).  
       Example 7  
     Determination of the Production Efficiency of Recombinant Viruses  
       [0073]    Recombinant viruses expressing GFP were prepared in this study. In order for this, 100 ng of pEntr_GFP gene cassette containing GFP genes and 200 ng of viral DNAs of Bsu36I treated vBacHTS, vBacHTS_His or vBacHTS_HisGST were reacted in the presence of 4 ul of integrase mixture comprising integrase, Xis (exisionase), IHF-α and IHF-β at 25° C. for 6 hrs. The resulting mixture was transferred to Sf21 cells together with lipofectin (from LTI) in order to generate recombinant baculoviruses. After incubating at 27° C. for 48 hrs, the expression of GFP was detected under fluorescence microscope. Since the cells, in which baculoviruses replicated, represented fluorescence, the efficiency of recombinant viral expression was determined by counting the cells showing green fluorescence. In the meantime, BacPAK6 viral DNAs (from Clontech) treated with Bsu36I and 500 ng of pBacPAK_GFP were transferred to Sf21 cells, then the expression of GFP in the Sf21 cells was monitored.  
                                                   TABLE 3                           The expression efficiency of GFP baculoviruses            Virus DNA   Bsu36I   gene cassette   GFP positive cell#                    BacPAK6   Yes   pBacPAK8 EGFP   154       VbacHTS       pEntr EGFP   1752       VbacHTS   yes   pEntr EGFP   572       VbacHTS His       pEntr EGFP   2236       VbacHTS His   yes   pEntr EGFP   654       VbacHTS HisGST       pEntr EGFP   1433       VbacHTS HisGST   yes   pEntr EGFP   475                  
 
         [0074]    As the result, the efficiency of the viral expression is much higher in the method of the present invention compared to the prior arts (see Table 3).  
       Example 8  
     Detection of the Efficiency of Recombinant Viral Expression  
       [0075]    Recombinant viruses expressing Gus were prepared, and the number of plaques expressing Gus genes was counted. In order for this, firstly, 100 ng of pEntr_Gus gene cassette containing Gus genes and 200 ng of each of viral DNAs of Bsu36I treated vBacHTS, vBacHTS_His or vBacHTS_HisGST were reacted in the presence of 4 ul of integrase mixture comprising integrase, exisionase, IHF-α and IHF-β at 25° C. for 6 hrs. The resulting mixture was transferred to Sf21 cells together with lipofectin (from LTI) to generate recombinant baculobiruses. Plaque assays were carried out for the viral culture medium. On the other hand, in order to detect productivity of the recombinant viruses, 4-day viral culture medium was dilated and transferred to insect cells. Subsequently, the cells were incubated for 4 days after adding medium containing 1% low-melting point agarose thereto. The numbers of plaques expressing Gus genes were counted after 4 days from the addition of 100 ul of 0.33% nutral red water solution and 25 ul of X-Gluc (20 mg/ml in DMSO) and afer subsequent dye treatment (see Table 4).  
                                         TABLE 4                           The efficiency of GUS recombinant viral expression                            Re-           Virus DNA   Bsu36I   gene cassette   Total   combinant   rate %               BacPAK6   yes   pBacPAK8   46   41   89%               Gus       VbacHTS       pEntr Gus   28   25   89%       VbacHTS   yes   pEntr Gus   57   57   100%        VbacHTS His       pEntr Gus   44   36   82%       VbacHTS His   yes   pEntr Gus   46   45   98%       VbacHTS HisGST       pEntr Gus   82   57   70%       VbacHTS HisGST   yes   pEntr Gus   32   32   100%        VbacHTS GFP       pEntr Gus   27   22   81%       VbacHTS GFP   yes   pEntr Gus   21   20   95%                  
 
         [0076]    As the result, most of the plaques represented blue color when they were treated with X-Gluc, which supported that the efficiency of GUS viral generation was very high in comparison to the prior arts.  
       Example 9  
     High-Throughput Preparation of Recombinant Viruses in Multi-Well Plate  
       [0077]    Desired recombinant viruses were prepared simultaneously from multi-well plate. For this purpose, the vBacHTS viral DNAs were treated with Bsu36I restriction enzyme. In this study, GFP gene cassette was employed to confirm the generation of recombinant baculoviruses and gene expression. The GFP gene cassettes were incubated in 96-deep well plate simultaneously and purified using automatic device. All the processes were carried out in multi-well plate while recombination reaction, insect cell incubation, and viral infection were performed using 8-channel pipette. About 50 ng of gene cassette and 200 ng of vBacHTS baculoviral DNAs were reacted in the presence of 2 ul of recombinase and 4 ul of buffer at 25° C. for 12 hrs. A total of 20 ul of reaction mixture was used in this reaction. In order to detect the insertion of gene cassette, PCR amplification was performed using primers for polyhedrin locus amplification.  
         [0078]    Baculo-Forward primer: 5-actgttttcgtaacagttttg-3 (SEQ. ID No.: 6)  
         [0079]    Baculo-Reverse primer: 5-acaacgcacagaatctagc-3 (SEQ. ID No.: 7)  
         [0080]    As the result, recombinant viral DNAs were generated with very high efficiency. Meanwhile, liposome was prepared by mixing 5 ul of the recombinant reaction mixture with 5 ul of 10% lipofectin dilates, which in turn was transferred to 50,000 of Sf21 cells in 96-well plate (SPL) followed by the incubation of the cells for 4 days. Protein expression was assayed after 3-day incubation of 50,000 of the Sf21 cells that were infected again with 10 ul of viruses. As for GFP viruses, the expression of green fluorescence was detected under the fluorescence microscope. And, the exhibition of symptoms of GFP viral infection was detected also. The cells were isolated from each of the wells with SDS-PAGE, and the expression of GFP was assayed using GFP-antibody with western blot. With regard to GFP proteins, the measured value showed similar pattern as detected under the fluorescence microscope (see FIG. 22).  
       Example 10  
     High-Throughput Preparation and Enzyme Activity Screening of LacZ Gene Mutants in 96-Well Plate Using GFP Recombinant Baculoviruses  
       [0081]    In order to prepare desired recombinant baculoviruses (sized about 1˜3.2 kb) from multi-well plate simultaneously, the vBacHTS_GFP viral DNAs were treated with Bsu36I restriction enzyme. Gene cassettes of pEntr_LacZdel were employed in this study. The gene cassettes were prepared in 96-well plate by PCR cloning of the gene of β-galactosidase from  E. coli  (sized about 3.2 kb), wherein the gene was deleted of 3′-terminus. More specifically, pEntr_LacZ was prepared by PCR cloning of gene of LacZ. Afterward, the pEntr_LacZ was treated with ExoIII/S1 deletion kit (#K0421) (from Fermentas) to generate of pEntr_LacZdel. All the processes were carried out in multi-well plate, while the recombination reaction, insect cell incubation and viral infection were performed with 8-channel pipette. 50 ng of gene cassettes and 200 ng of vBacHTS baculoviral DNAs were reacted in the presence of 2 ul of recombines and 4 ul of buffer at 25° C. for 12 hrs. A total of 20 ul of reaction mixture was used in this reaction. In order to detect the insertion of gene cassette, PCR amplification was performed using a pair of primers (actgttttcgtaacagttttg and acaacgcacagaatctagc) for the amplification of polyhedrin locus. As the result, recombinant viruses were generated at very high efficiency (see FIG. 25). Meanwhile, liposome was prepared by mixing 5 ul of the recombinant reaction mixture and 5 ul of 10% lipofectin dilates, which in turn was transferred to 50,000 of Sf21 cells in 96-well plate (SPL) followed by incubation of the cells for 4 days (see FIG. 26). In order to assay the expression of GFP fusion proteins, SDS-PAGE analysis and western blotting using GFP antibodies were carried out (see FIG. 26).  
         [0082]    In order to screen the gene activities, 1.5 ul of X-Gal (25 mg/ml in DMSO) of developer of β-galactosidase was added to each well that contained viruses incubated for 3 days after the primary infection. After 12 hrs of reaction, viruses containing β-galactosidase activity were detected in 2 (two) wells (see FIG. 27).  
       Example 11  
     High-Throughput Heterologous Gene Expression Using Recombinant Baculoviruses in 96-Well Plate and the Screening of Enzyme Activity Using the Same  
       [0083]    [0083] S. cerevisie , which includes 124 protein kinase genes, was employed in this study. PCR amplification and cloning were carried out using gene-specific primers to obtain 112 gene cassettes from the  S. cerevisie  genome. Recombinant baculoviruses were prepared by the recombination of the gene cassettes and vBacHTS_GFP vector DNA. The respective recombinant viruses represented particular fluorescence according to the proteins fused to GFP (see FIG. 28). Also, the recombinant baculoviruses having 32 human cDNA were prepared in the same manner.  
         [0084]    Firstly, cells were infected with these viruses and incubated for 3 days. The cell lysate was generated using a buffer to lyse cells. Viruses, which exhibited protein kinase activities to substrates of Histon H1, MBP (myelin basic protein) and Rb, were screened. 2 ug of protein substrates (histon H1, MBP and Rb) were reacted with 10 ul of cell extracts at 30° C. for 10 min, in the presence of 10 uM ATP, 0.2 uCi of P32-δ-labeled ATP (gamma, P32 ATP), and 2 ul of phosphate buffer [200 mM Tris-HCl (pH=8.0), 100 mM of MgCl 2 , 10 mM of EGTA and 10 mM of DTT]. After stopping the reaction by adding 120 ul of 1% phosphoric acid solution, the reaction mixture was transferred to PVDF membrane (from Milipore, #MAIP-N45). Afterward, the membrane was washed with detergent [10 mM Tris-HCl (pH-8.0), 1 mM EDTA and 150 mM NaCl] four times and dried, which in turn was exposed to phosphoscreen at room temperature, and then signals were detected with Phosphoimager (from MD or Fuji). As the result, it was observed that protein kinase activities were increased in some viruses (see FIG. 29). Subsequently, SDS-PAGE electophoretic analysis was carried out to confirm the specificity of protein phosphorylation. At that time, the level of Rb protein phosphorylation with regard to the concentration of protein kinase included in cell extracts was detected, while varying the concentrations of cell extracts. As the result, only the cell extracts of the baculoviruses expressing plate#2-D3 protein and plate#2-A9 protein increased Rb protein phosphorylation in a concentration dependent manner (see FIG. 30). Meanwhile, proteins of plate#2-D1 and plate#2-D2 exhibited very weak phosphorylation activities. And, it was acknowledged that the positive signals from the proteins of plate#2-D1 and plate#2-D2 were resulted from auto-phosphorylation activities rather than from Rb protein phosphorylation. Rb protein phosphorylations by plate#2-D3 and plate#2-A9 proteins exhibited a typical protein phosphorylation pattern in that phosphorylation of Rb protein was dependent on the concentrations of the substrate and the enzyme (FIG. 31). Thus, it is possible to find out new useful proteins from baculoviral libraries obtained from the high-throughput system of the present invention according to the present invention.  
       Example 12  
     The Preparation of pBacHTS2 and pBacHTS2 GFP  
       [0085]    Baculoviral transfer vector of pBacHTS2 was prepared in order to generate vBacHTS2 viruses (SEQ. ID No.8: the sequences of its polyhedrin locus) that can replicate and can be applied to recombination reaction in vitro. The pBacHTS2 had BAC vector originated replication origin and CmR (chloramphenichol resistance gene) at Bsu36I restriction enzyme recognition site located between attR1 site and attR2 site thereof. Thus, it was a kind of BAC vectors that can replicate in bacteria. Replacing the BAC vector originated replication origin and CmR with desired gene cassette generated vBacHTS viruses in vitro.  
         [0086]    In order to prepare pBacHTS2, firstly, 6.5 kb of DNA fragment containing BAC (Bacterial artificial chromosome) vector replication origin and CmR was generated using pBACe3.6 (Genbank:U80929) as a template. Then, PCR amplification was carried out using 10 pmole of a pair of sequences having Bsu36I recognition sequences, 200 uM of dNTPs and 2.5 U of Pfu turbo polymerase (Stratagene) with PCR cycler (Applied Biosystem, Gene Amp PCR System 2700). A total of 50 ul of reaction mixture was used. PCR was carried out for 20 cycles (DNA denaturation at 95° C., 30 sec, DNA extension at 60° C., 30 sec, DNA amplification at 72° C., 7 min). PCR products of 6.5 kb of DNA fragment and vBacHTS vector were digested by Bsu36I, and were reacted in the presence of T4 ligase at 16° C. overnight.  E. coli  (DH5a) was transformed using the resulting mixture, which in turn was incubated with Cm and Amp medium (for selection) to produce pBacHTS2. Likewise, Bsu36I treated PCR products of 6.5 kb of DNA fragment were inserted to Bsu36I site of the pBacHTS_GFP vector so as to obtain pBacHTS2_GFP vector.  
       Example 13  
     Preparation of Viruses Having Site-Specific Recombination Sites  
       [0087]    Baculoviruses were prepared using homologous recombination, which replicated in bacteria. Briefly, the homologous recombination was induced in Sf21 cells by transferring a mixture of pBacHTS2 plasmid, Bsu36I digested BacPAK6 DNA and Lipofectin (from LTI). The infected cells were incubated at 27° C. for 4 days, and were transferred to 5×10 6  of Sf21 cells (in 100 mm of dish). Thereby, high titer of viruses was obtained. Then, precipitates of viral particles were produced by centrifugation after adding 2 ml of 50% PEG6000 to the cell culture medium. Next, proteinase K was added to the mixture at 42° C. and left the mixture alone for 2 hrs to dismantle viral envelope. The viral lysate was extracted twice using the same amount of phenol/chloroform, and ethanol was added thereto. Thereby, precipitated and purified vBacHTS2 viral DNAs (SEQ. ID No.8: the sequences of its polyhedrin locus) were obtained. Then, cell lines (from LTI) were transformed with the purified DNAs using MicroPulser (from Bio-Rad), which are in turn incubated in Cm medium to form bacterial colony. The bacteria were incubated in 1 liter of 2×YT medium (10 g of Yeast extract powder, 16 g of tryptone, 5 g of NaCl within per 1 (one) Liter), then DNAs were purified and named as bBacHTS2 (FIG. 16). Thus, according to this method, it is possible to reduce the required time-period and cost significantly, since the viral DNAs can be obtained from bacterial culture without the need of incubation in insect cells. Purified 10 ug of DNAs was digested with 50 unit of Bsu36I at 37° C. for 5 hrs, which in turn was heat treated at 80° C. for 20 min to inactivate Bsu361 enzyme. Likewise, vBacHTS2_GFP (SEQ. ID No.:9) was prepared from pBacHTS2_GFP vector by homologous recombination; and then was transferred to DH10B by electrophoration. The DNAs were purified and named as bBacHTS2_GFP (see FIG. 16).  
       Example 14  
     Preparation of the Desired Recombinant Viruses Using vBacHTS2 and vBacHTS2 GFP DNAs Generated from Bacteria  
       [0088]    50 ng of the pEntr_GFP and the pEntr_Gus gene cassettes were reacted with 200 ng of Bsu36I treated vBacHTS2 viral DNAs in the presence of 1 ul of integrase mixture in vitro at 25° C. for 12 hrs. The same reaction was carried out except for the use of the pEntr_Gus gene cassette instead of pEntr_GFP gene cassette. PCR was performed as disclosed in the Examples mentioned above. PCR amplification was carried out using the reaction mixture, as templates, and a pair of primers amplifying viral polyhedrin locus to confirm whether recombination reaction occurred. A total of 20 cycles of PCR were carried out using each of 10 pmole of the primers and 1 tube of Bioneer premix kit, in which one cycle of PCR was performed at 95° C. for 30 sec, 50° C. for 30 see and 72° C. for 3 min. After completing PCR reaction, electrophoresis was carried out for the PCR products on 1% agarose gel to confirm efficient performance of recombination. The gene cassette reaction mixture was transferred to Sf21 cells together with 5 ul of Lipofectin (from LTI) so as to obtain baculoviruses.  
         [0089]    Green fluorescence was observed in insect cells, after 2 days from transferring GFP gene cassette reaction mixture thereto. In addition, most of the cells represented green fluorescence after 4 days from transfer (see FIG. 25). Sf21 cells were infected again with the viral culture medium to confirm green fluorescence and the symptoms of infection. Likewise, pEntr_Gus gene cassette was reacted with Bsu36I treated vBacHTS2 DNAs at 25° C. for 12 hrs. Then, the obtained reaction mixture was transferred to Sf21 cells and was incubated at 27° C. for 4 days. When 6 ul of X-Gluc (20 mg/ml in DMSO) was added to incubated cells, all the culture medium represented strong blue, which supported not only the expressions of Gus genes but also efficient generation of recombinant viruses containing Gus genes.  
     
       
       
         1 
         
           
             9  
           
           
             1  
             10000  
             DNA  
             Artificial Sequence  
             
               Polyhedrin locus of vBacHTS virus  
             
           
            1 

gaattctacc cgtaaagcga gtttagtttt gaaaaacaaa tgacatcatt tgtataatga     60 

catcatcccc tgattgtgtt ttacaagtag aattctatcc gtaaagcgag ttcagttttg    120 

aaaacaaatg agtcatacct aaacacgtta ataatcttct gatatcagct tatgactcaa    180 

gttatgagcc gtgtgcaaaa catgagataa gtttatgaca tcatccactg atcgtgcgtt    240 

acaagtagaa ttctactcgt aaagccagtt cggttatgag ccgtgtgcaa aacatgacat    300 

cagcttatga ctcatacttg attgtgtttt acgcgtagaa ttctactcgt aaagcgagtt    360 

cggttatgag ccgtgtgcaa aacatgacat cagcttatga gtcataatta atcgtgcgtt    420 

acaagtagaa ttctactcgt aaagcgagtt gaaggatcat atttagttgc gtttatgaga    480 

taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt tacaatgcgg    540 

ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc ccgagttgtt    600 

tgcgtacgtg actagcgaag aagatgtgtg gaccgcagaa cagatagtaa aacaaaaccc    660 

tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg gtgtgcattt    720 

tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt tgccgcctga    780 

aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt gtcccggcat    840 

gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg tgtgcagata    900 

tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg aaaaagccag    960 

aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat taatattatt   1020 

tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt ccagcgaacc   1080 

aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt gttccaatcg   1140 

acggtaggat taggccggat attctccacc acaatgttgg caacgttgat gttacgttta   1200 

tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt agtgccgtcg   1260 

cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac cgcgcgatcc   1320 

gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt ctgcattatt   1380 

tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag atcagtcatg   1440 

acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc aacgatgcgt   1500 

tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac cgcttgcagc   1560 

gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt gctctcctcc   1620 

tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg aattacttct   1680 

tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat aatcagctga   1740 

atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg gtcgcccctt   1800 

ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa taacgatttg   1860 

tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt taaactgtta   1920 

gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta ccgcaggttg   1980 

aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt gatacacgtg   2040 

tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt ggtaagcaat   2100 

aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg cagacggcgc   2160 

cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac atcgcaaaag   2220 

ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc ttatttaata   2280 

aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc gagcagttcg   2340 

ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac cagcggcgtg   2400 

ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg cacgtcggcc   2460 

tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt gcgcatatct   2520 

atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga aattaaatca   2580 

ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc gtcgattaaa   2640 

tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta ttcccgagtc   2700 

aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat ttaatgcaac   2760 

tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc cgttgtcgca   2820 

tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt gcgacgcaac   2880 

gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt tttacgaagc   2940 

gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga ctacaaaatt   3000 

accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt agtcgaatca   3060 

ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg tgtggagtcc   3120 

gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc ccgatgattt   3180 

tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg ttttggtcaa   3240 

aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta atgaaattaa   3300 

aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat gcgtagaagg   3360 

aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt gtataaaaaa   3420 

aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca ggaagaggtt   3480 

tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt   3540 

aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg aagtcatgca   3600 

tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt   3660 

cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt gtaattattg   3720 

aataataaaa caattataaa tgctaaattt gttttttatt aacgatacaa accaaacgca   3780 

acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc gctgaggtaa   3840 

tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat tttattttca   3900 

cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc atttttctcc   3960 

tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta tagagtaaat   4020 

tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc gctgcgcata   4080 

gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg tgttgcttta   4140 

attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg   4200 

gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac cggattaaca   4260 

taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc cttttctata   4320 

ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat gagacgcaca   4380 

aactaatatc acaaactgga aatgtctatc aatatatagt tgctgatatc atggagataa   4440 

ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt   4500 

aataaaaaaa cctataaata cggatcccgg ggtaccacaa gtttgtacaa aaaagctgaa   4560 

cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag   4620 

actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc   4680 

cttaggaatt cgcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg   4740 

cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgcttct   4800 

agaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat   4860 

gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca   4920 

cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa   4980 

agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa   5040 

gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg   5100 

ggaatataga attcctcagg tcgaccatag tgactggata tgttgtgttt tacagtatta   5160 

tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt   5220 

ttctcgttca gctttcttgt acaaagtggt ctcgagttaa ttaattgatc cgggttatta   5280 

gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta   5340 

ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg   5400 

attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag   5460 

gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg   5520 

attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat   5580 

attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt   5640 

tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaataa   5700 

agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc   5760 

ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct   5820 

attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat   5880 

ttctgattgc gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga   5940 

caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa   6000 

tggcggcggt ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg   6060 

cggcggaggc ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt   6120 

ctctttaggc aacacagtcg gcacctcaac tattgtactg gtttcgggcg ccgtttttgg   6180 

tttgaccggt ctgagacgag tgcgattttt ttcgtttcta atagcttcca acaattgttg   6240 

tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc attggtggag cgggcggcaa   6300 

ttcagacatc gatggtggtg gtggtggtgg aggcgctgga atgttaggca cgggagaagg   6360 

tggtggcggc ggtgccgccg gtataatttg ttctggttta gtttgttcgc gcacgattgt   6420 

gggcaccggc gcaggcgccg ctggctgcac aacggaaggt cgtctgcttc gaggcagcgc   6480 

ttggggtggt ggcaattcaa tattataatt ggaatacaaa tcgtaaaaat ctgctataag   6540 

cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac aaccgctcaa tgtaagcaat   6600 

tgtattgtaa agagattgtc tcaagctcgg atcccgcacg ccgataacaa gccttttcat   6660 

ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgacgtaca tgtatgcttt   6720 

gttgtcaaaa acgtcgttgg caagctttaa aatatttaaa agaacatctc tgttcagcac   6780 

cactgtgttg tcgtaaatgt tgtttttgat aatttgcgct tccgcagtat cgacacgttc   6840 

aaaaaattga tgcgcatcaa ttttgttgtt cctattattg aataaataag attgtacaga   6900 

ttcatatcta cgattcgtca tggccaccac aaatgctacg ctgcaaacgc tggtacaatt   6960 

ttacgaaaac tgcaaaaacg tcaaaactcg gtataaaata atcaacgggc gctttggcaa   7020 

aatatctatt ttatcgcaca agcccactag caaattgtat ttgcagaaaa caatttcggc   7080 

gcacaatttt aacgctgacg aaataaaagt tcaccagtta atgagcgacc acccaaattt   7140 

tataaaaatc tattttaatc acggttccat caacaaccaa gtgatcgtga tggactacat   7200 

tgactgtccc gatttatttg aaacactaca aattaaaggc gagctttcgt accaacttgt   7260 

tagcaatatt attagacagc tgtgtgaagc gctcaacgat ttgcacaagc acaatttcat   7320 

acacaacgac ataaaactcg aaaatgtctt atatttcgaa gcacttgatc gcgtgtatgt   7380 

ttgcgattac ggattgtgca aacacgaaaa ctcacttagc gtgcacgacg gcacgttgga   7440 

gtattttagt ccggaaaaaa ttcgacacac aactatgcac gtttcgtttg actggtacgc   7500 

cgtcggcgtg ttaacataca agttgctaac cggcggccga cacccatttg aaaaaagcga   7560 

agacgaaatg ttggacttga atagcatgaa gcgtcgtcag caatacaatg acattggcgt   7620 

tttaaaacac gttcgtaacg ttaacgctcg tgactttgtg tactgcctaa caagatacaa   7680 

catagattgt agactcacaa attacaaaca aattataaaa catgagtttt tgtcgtaaaa   7740 

atgccacttg ttttacgagt agaattctac gtgtaacaca cgatctaaaa gatgatgtca   7800 

ttttttatca atgactcatt tgttttaaaa cagacttgtt ttacgagtag aattctacgt   7860 

gtaaagcatg atcgtgagtg gtgttaataa aatcataaaa attattgtaa atgtttatta   7920 

tttaaaaacg attcaaatat ataataaaaa caatctacat ctatttcttc acaatccata   7980 

acacacaaca ggtccatcaa tgagtttttg tctttatccg acatactatg tgcatgtaac   8040 

aaatcaaata catcttttaa atttttatac acatctttac attgtctacc aaaatcttta   8100 

ataaccctat aacaaggaaa agacttttct tcttgcgtgg ttttgccgcg cagatattga   8160 

aataaaatgt gcatgcacga caacttgtgt ttactaaaat gctccttgcc tataccgcaa   8220 

aaccggccat acatttcggc gattacacgc ggacaattgt acgattcgtc tacgtgtaaa   8280 

cgatcatcat aatcactctt gcgcaaacga ataaattttt tcaccgcttc cgacaaacga   8340 

ggcaccaatt cggcgggcac gcttcgatac attattctgt gcacataagt taccacacaa   8400 

aatttattgt accaccatcc gacaacgtcg ttattagggt tgaacacgtt ggcgatgcgc   8460 

agcagtttcc cgtttctcat gaaatattca aagcggccca aaataatttg caagcaatcc   8520 

aacatgtctt gagaaatttc tcgttcaaaa ttgttcaaag agaatatctg ccatccgttt   8580 

tgaacgcgca cgctgacggg aaccaccgca tcgatttgct ccaacacttc acggacgtta   8640 

tcgtcgatgc ccatcgtttc gctggtgctg aaccaatggg aaaggctctt gatggaatcg   8700 

cccgcgtcta tcatcttgac cgcttcgtca aaggtgcaac tgccgctctt caaacgccgc   8760 

atagcggtca cgtcccgctc tatgcacgac ataccgttta cgtacgattc tgataggtat   8820 

tcctgaacta tacggtaatg gtgatacgac tcgccataca cgtcgtgcac ctcattgtat   8880 

ttagcataat aattgtaaat tattaacttt gcagcgagag acatgttgtc agtaaagcgg   8940 

tgctaggctc aataatactg atgtacaggc acgcgtgcta tttatatata atttcgcaag   9000 

gaggggagct gttatcggtt gctattatta aagaatggcc gtctgttttt atcacaagct   9060 

tggcagcctc aaccatgaag cgtcgtcatt gtaaattaaa ttctctgcct caagaattat   9120 

ttgacaagat tgtcgagtat ttatctttat ctgattactg caatttggtg cttgtctgta   9180 

aaagaccttc tagtaaatat aacgtgatat ttgatagtac taatcaccaa catttgaaag   9240 

gcgtgtacaa aaagacagac gtgcaaataa caagctacaa cgaatacatc aactgtattt   9300 

gcaacgaact gagacaagac gaattctatg ccaaatcatc atggattgcg agtatttgcg   9360 

gtcaccagag agcgacaatt tttagtgtaa caaataaaca agtagaaatg aaatatcatt   9420 

tgtataatat agcaattgtg gaaagtgaag attgcaacgg attttaccca tttgagccaa   9480 

cgcgcgattg tttaatatgc aaacaaaaaa accaatgtcc tcgtaattca tttattgttt   9540 

cgttgtgtaa atatttagaa aaacaaaatg tacaatcaaa ctttatatat tatttatacg   9600 

aaataaatac ataataataa ctattataca tgtttttatt ttacaatact tcctgtataa   9660 

cctctctaac tacattagga gtacaatcca cgtcaattac acgtttagct atttttctaa   9720 

ttttgtaatg tttatcgtag agtttttcgt taatacattg aatagccaac aagggatttg   9780 

ggtgcacacc gtcatagagt acttccatgt cgtcttcaaa gcgcattttt cgcttgcgaa   9840 

aatgccgctc ttggcccaaa acaaaagcga gtttgatgcg gtcgtcgatg cgttccgaaa   9900 

atacggccaa atgctggtgt ttggtgatgt cgcgcggaaa cgtcaccgtg ccatttttgc   9960 

tttccgccac gacggcggtt ttcaattttt cggccgactg                        10000 

 
           
             2  
             10000  
             DNA  
             Artificial Sequence  
             
               Polyhedrin locus of vBacHTS_His  
             
           
            2 

gaattctacc cgtaaagcga gtttagtttt gaaaaacaaa tgacatcatt tgtataatga     60 

catcatcccc tgattgtgtt ttacaagtag aattctatcc gtaaagcgag ttcagttttg    120 

aaaacaaatg agtcatacct aaacacgtta ataatcttct gatatcagct tatgactcaa    180 

gttatgagcc gtgtgcaaaa catgagataa gtttatgaca tcatccactg atcgtgcgtt    240 

acaagtagaa ttctactcgt aaagccagtt cggttatgag ccgtgtgcaa aacatgacat    300 

cagcttatga ctcatacttg attgtgtttt acgcgtagaa ttctactcgt aaagcgagtt    360 

cggttatgag ccgtgtgcaa aacatgacat cagcttatga gtcataatta atcgtgcgtt    420 

acaagtagaa ttctactcgt aaagcgagtt gaaggatcat atttagttgc gtttatgaga    480 

taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt tacaatgcgg    540 

ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc ccgagttgtt    600 

tgcgtacgtg actagcgaag aagatgtgtg gaccgcagaa cagatagtaa aacaaaaccc    660 

tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg gtgtgcattt    720 

tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt tgccgcctga    780 

aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt gtcccggcat    840 

gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg tgtgcagata    900 

tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg aaaaagccag    960 

aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat taatattatt   1020 

tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt ccagcgaacc   1080 

aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt gttccaatcg   1140 

acggtaggat taggccggat attctccacc acaatgttgg caacgttgat gttacgttta   1200 

tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt agtgccgtcg   1260 

cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac cgcgcgatcc   1320 

gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt ctgcattatt   1380 

tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag atcagtcatg   1440 

acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc aacgatgcgt   1500 

tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac cgcttgcagc   1560 

gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt gctctcctcc   1620 

tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg aattacttct   1680 

tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat aatcagctga   1740 

atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg gtcgcccctt   1800 

ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa taacgatttg   1860 

tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt taaactgtta   1920 

gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta ccgcaggttg   1980 

aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt gatacacgtg   2040 

tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt ggtaagcaat   2100 

aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg cagacggcgc   2160 

cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac atcgcaaaag   2220 

ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc ttatttaata   2280 

aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc gagcagttcg   2340 

ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac cagcggcgtg   2400 

ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg cacgtcggcc   2460 

tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt gcgcatatct   2520 

atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga aattaaatca   2580 

ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc gtcgattaaa   2640 

tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta ttcccgagtc   2700 

aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat ttaatgcaac   2760 

tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc cgttgtcgca   2820 

tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt gcgacgcaac   2880 

gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt tttacgaagc   2940 

gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga ctacaaaatt   3000 

accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt agtcgaatca   3060 

ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg tgtggagtcc   3120 

gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc ccgatgattt   3180 

tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg ttttggtcaa   3240 

aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta atgaaattaa   3300 

aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat gcgtagaagg   3360 

aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt gtataaaaaa   3420 

aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca ggaagaggtt   3480 

tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt   3540 

aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg aagtcatgca   3600 

tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt   3660 

cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt gtaattattg   3720 

aataataaaa caattataaa tgctaaattt gttttttatt aacgatacaa accaaacgca   3780 

acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc gctgaggtaa   3840 

tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat tttattttca   3900 

cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc atttttctcc   3960 

tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta tagagtaaat   4020 

tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc gctgcgcata   4080 

gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg tgttgcttta   4140 

attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg   4200 

gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac cggattaaca   4260 

taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc cttttctata   4320 

ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat gagacgcaca   4380 

aactaatatc acaaactgga aatgtctatc aatatatagt tgctgatatc atggagataa   4440 

ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt   4500 

aataaaaaaa cctataaata cggatctatg catcatcacc atcatcacgg atcccggggt   4560 

accacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata   4620 

ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag   4680 

tcactatggc ggccgcatta ggcacccctt aggaattcgc ttactaaaag ccagataaca   4740 

gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg   4800 

aagtatgtca aaaagaggtg tgcttctaga atgcagttta aggtttacac ctataaaaga   4860 

gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga   4920 

cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt   4980 

tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt   5040 

gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc   5100 

aaaaacgcca ttaacctgat gttctgggga atatagaatt cctcaggtcg accatagtga   5160 

ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa   5220 

tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtctc   5280 

gagttaatta attgatccgg gttattagta catttattaa gcgctagatt ctgtgcgttg   5340 

ttgatttaca gacaattgtt gtacgtattt taataattca ttaaatttat aatctttagg   5400 

gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct ttatatctga atttaaatat   5460 

taaatcctca atagatttgt aaaataggtt tcgattagtt tcaaacaagg gttgtttttc   5520 

cgaaccgatg gctggactat ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc   5580 

ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc   5640 

gacgtcgttc aaaatattat gcgcttttgt atttctttca tcactgtcgt tagtgtacaa   5700 

ttgactcgac gtaaacacgt taaataaagc ttggacatat ttaacatcgg gcgtgttagc   5760 

tttattaggc cgattatcgt cgtcgtccca accctcgtcg ttagaagttg cttccgaaga   5820 

cgattttgcc atagccacac gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa   5880 

atttgtagtt gagctttttg gaattatttc tgattgcggg cgtttttggg cgggtttcaa   5940 

tctaactgtg cccgatttta attcagacaa cacgttagaa agcgatggtg caggcggtgg   6000 

taacatttca gacggcaaat ctactaatgg cggcggtggt ggagctgatg ataaatctac   6060 

catcggtgga ggcgcaggcg gggctggcgg cggaggcgga ggcggaggtg gtggcggtga   6120 

tgcagacggc ggtttaggct caaatgtctc tttaggcaac acagtcggca cctcaactat   6180 

tgtactggtt tcgggcgccg tttttggttt gaccggtctg agacgagtgc gatttttttc   6240 

gtttctaata gcttccaaca attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc   6300 

cgtcggcatt ggtggagcgg gcggcaattc agacatcgat ggtggtggtg gtggtggagg   6360 

cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt gccgccggta taatttgttc   6420 

tggtttagtt tgttcgcgca cgattgtggg caccggcgca ggcgccgctg gctgcacaac   6480 

ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc aattcaatat tataattgga   6540 

atacaaatcg taaaaatctg ctataagcat tgtaatttcg ctatcgttta ccgtgccgat   6600 

atttaacaac cgctcaatgt aagcaattgt attgtaaaga gattgtctca agctcggatc   6660 

ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc   6720 

gctgtcgtcg acgtacatgt atgctttgtt gtcaaaaacg tcgttggcaa gctttaaaat   6780 

atttaaaaga acatctctgt tcagcaccac tgtgttgtcg taaatgttgt ttttgataat   6840 

ttgcgcttcc gcagtatcga cacgttcaaa aaattgatgc gcatcaattt tgttgttcct   6900 

attattgaat aaataagatt gtacagattc atatctacga ttcgtcatgg ccaccacaaa   6960 

tgctacgctg caaacgctgg tacaatttta cgaaaactgc aaaaacgtca aaactcggta   7020 

taaaataatc aacgggcgct ttggcaaaat atctatttta tcgcacaagc ccactagcaa   7080 

attgtatttg cagaaaacaa tttcggcgca caattttaac gctgacgaaa taaaagttca   7140 

ccagttaatg agcgaccacc caaattttat aaaaatctat tttaatcacg gttccatcaa   7200 

caaccaagtg atcgtgatgg actacattga ctgtcccgat ttatttgaaa cactacaaat   7260 

taaaggcgag ctttcgtacc aacttgttag caatattatt agacagctgt gtgaagcgct   7320 

caacgatttg cacaagcaca atttcataca caacgacata aaactcgaaa atgtcttata   7380 

tttcgaagca cttgatcgcg tgtatgtttg cgattacgga ttgtgcaaac acgaaaactc   7440 

acttagcgtg cacgacggca cgttggagta ttttagtccg gaaaaaattc gacacacaac   7500 

tatgcacgtt tcgtttgact ggtacgccgt cggcgtgtta acatacaagt tgctaaccgg   7560 

cggccgacac ccatttgaaa aaagcgaaga cgaaatgttg gacttgaata gcatgaagcg   7620 

tcgtcagcaa tacaatgaca ttggcgtttt aaaacacgtt cgtaacgtta acgctcgtga   7680 

ctttgtgtac tgcctaacaa gatacaacat agattgtaga ctcacaaatt acaaacaaat   7740 

tataaaacat gagtttttgt cgtaaaaatg ccacttgttt tacgagtaga attctacgtg   7800 

taacacacga tctaaaagat gatgtcattt tttatcaatg actcatttgt tttaaaacag   7860 

acttgtttta cgagtagaat tctacgtgta aagcatgatc gtgagtggtg ttaataaaat   7920 

cataaaaatt attgtaaatg tttattattt aaaaacgatt caaatatata ataaaaacaa   7980 

tctacatcta tttcttcaca atccataaca cacaacaggt ccatcaatga gtttttgtct   8040 

ttatccgaca tactatgtgc atgtaacaaa tcaaatacat cttttaaatt tttatacaca   8100 

tctttacatt gtctaccaaa atctttaata accctataac aaggaaaaga cttttcttct   8160 

tgcgtggttt tgccgcgcag atattgaaat aaaatgtgca tgcacgacaa cttgtgttta   8220 

ctaaaatgct ccttgcctat accgcaaaac cggccataca tttcggcgat tacacgcgga   8280 

caattgtacg attcgtctac gtgtaaacga tcatcataat cactcttgcg caaacgaata   8340 

aattttttca ccgcttccga caaacgaggc accaattcgg cgggcacgct tcgatacatt   8400 

attctgtgca cataagttac cacacaaaat ttattgtacc accatccgac aacgtcgtta   8460 

ttagggttga acacgttggc gatgcgcagc agtttcccgt ttctcatgaa atattcaaag   8520 

cggcccaaaa taatttgcaa gcaatccaac atgtcttgag aaatttctcg ttcaaaattg   8580 

ttcaaagaga atatctgcca tccgttttga acgcgcacgc tgacgggaac caccgcatcg   8640 

atttgctcca acacttcacg gacgttatcg tcgatgccca tcgtttcgct ggtgctgaac   8700 

caatgggaaa ggctcttgat ggaatcgccc gcgtctatca tcttgaccgc ttcgtcaaag   8760 

gtgcaactgc cgctcttcaa acgccgcata gcggtcacgt cccgctctat gcacgacata   8820 

ccgtttacgt acgattctga taggtattcc tgaactatac ggtaatggtg atacgactcg   8880 

ccatacacgt cgtgcacctc attgtattta gcataataat tgtaaattat taactttgca   8940 

gcgagagaca tgttgtcagt aaagcggtgc taggctcaat aatactgatg tacaggcacg   9000 

cgtgctattt atatataatt tcgcaaggag gggagctgtt atcggttgct attattaaag   9060 

aatggccgtc tgtttttatc acaagcttgg cagcctcaac catgaagcgt cgtcattgta   9120 

aattaaattc tctgcctcaa gaattatttg acaagattgt cgagtattta tctttatctg   9180 

attactgcaa tttggtgctt gtctgtaaaa gaccttctag taaatataac gtgatatttg   9240 

atagtactaa tcaccaacat ttgaaaggcg tgtacaaaaa gacagacgtg caaataacaa   9300 

gctacaacga atacatcaac tgtatttgca acgaactgag acaagacgaa ttctatgcca   9360 

aatcatcatg gattgcgagt atttgcggtc accagagagc gacaattttt agtgtaacaa   9420 

ataaacaagt agaaatgaaa tatcatttgt ataatatagc aattgtggaa agtgaagatt   9480 

gcaacggatt ttacccattt gagccaacgc gcgattgttt aatatgcaaa caaaaaaacc   9540 

aatgtcctcg taattcattt attgtttcgt tgtgtaaata tttagaaaaa caaaatgtac   9600 

aatcaaactt tatatattat ttatacgaaa taaatacata ataataacta ttatacatgt   9660 

ttttatttta caatacttcc tgtataacct ctctaactac attaggagta caatccacgt   9720 

caattacacg tttagctatt tttctaattt tgtaatgttt atcgtagagt ttttcgttaa   9780 

tacattgaat agccaacaag ggatttgggt gcacaccgtc atagagtact tccatgtcgt   9840 

cttcaaagcg catttttcgc ttgcgaaaat gccgctcttg gcccaaaaca aaagcgagtt   9900 

gatgcggtc gtcgatgcgt tccgaaaata cggccaaatg ctggtgtttg gtgatgtcgc    9960 

cggaaacgt caccgtgcca tttttgcttt ccgccacgac                         10000 

 
           
             3  
             10000  
             DNA  
             Artificial Sequence  
             
               Polyhedrin locus of vBacHTS_HisGst  
             
           
            3 

gaattctacc cgtaaagcga gtttagtttt gaaaaacaaa tgacatcatt tgtataatga     60 

catcatcccc tgattgtgtt ttacaagtag aattctatcc gtaaagcgag ttcagttttg    120 

aaaacaaatg agtcatacct aaacacgtta ataatcttct gatatcagct tatgactcaa    180 

gttatgagcc gtgtgcaaaa catgagataa gtttatgaca tcatccactg atcgtgcgtt    240 

acaagtagaa ttctactcgt aaagccagtt cggttatgag ccgtgtgcaa aacatgacat    300 

cagcttatga ctcatacttg attgtgtttt acgcgtagaa ttctactcgt aaagcgagtt    360 

cggttatgag ccgtgtgcaa aacatgacat cagcttatga gtcataatta atcgtgcgtt    420 

acaagtagaa ttctactcgt aaagcgagtt gaaggatcat atttagttgc gtttatgaga    480 

taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt tacaatgcgg    540 

ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc ccgagttgtt    600 

tgcgtacgtg actagcgaag aagatgtgtg gaccgcagaa cagatagtaa aacaaaaccc    660 

tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg gtgtgcattt    720 

tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt tgccgcctga    780 

aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt gtcccggcat    840 

gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg tgtgcagata    900 

tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg aaaaagccag    960 

aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat taatattatt   1020 

tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt ccagcgaacc   1080 

aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt gttccaatcg   1140 

acggtaggat taggccggat attctccacc acaatgttgg caacgttgat gttacgttta   1200 

tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt agtgccgtcg   1260 

cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac cgcgcgatcc   1320 

gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt ctgcattatt   1380 

tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag atcagtcatg   1440 

acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc aacgatgcgt   1500 

tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac cgcttgcagc   1560 

gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt gctctcctcc   1620 

tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg aattacttct   1680 

tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat aatcagctga   1740 

atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg gtcgcccctt   1800 

ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa taacgatttg   1860 

tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt taaactgtta   1920 

gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta ccgcaggttg   1980 

aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt gatacacgtg   2040 

tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt ggtaagcaat   2100 

aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg cagacggcgc   2160 

cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac atcgcaaaag   2220 

ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc ttatttaata   2280 

aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc gagcagttcg   2340 

ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac cagcggcgtg   2400 

ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg cacgtcggcc   2460 

tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt gcgcatatct   2520 

atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga aattaaatca   2580 

ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc gtcgattaaa   2640 

tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta ttcccgagtc   2700 

aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat ttaatgcaac   2760 

tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc cgttgtcgca   2820 

tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt gcgacgcaac   2880 

gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt tttacgaagc   2940 

gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga ctacaaaatt   3000 

accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt agtcgaatca   3060 

ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg tgtggagtcc   3120 

gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc ccgatgattt   3180 

tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg ttttggtcaa   3240 

aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta atgaaattaa   3300 

aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat gcgtagaagg   3360 

aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt gtataaaaaa   3420 

aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca ggaagaggtt   3480 

tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt   3540 

aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg aagtcatgca   3600 

tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt   3660 

cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt gtaattattg   3720 

aataataaaa caattataaa tgctaaattt gttttttatt aacgatacaa accaaacgca   3780 

acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc gctgaggtaa   3840 

tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat tttattttca   3900 

cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc atttttctcc   3960 

tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta tagagtaaat   4020 

tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc gctgcgcata   4080 

gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg tgttgcttta   4140 

attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg   4200 

gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac cggattaaca   4260 

taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc cttttctata   4320 

ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat gagacgcaca   4380 

aactaatatc acaaactgga aatgtctatc aatatatagt tgctgatatc atggagataa   4440 

ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt   4500 

aataaaaaaa cctataaata cggatctatg catcatcacc atcatcacgg atctatgtcc   4560 

cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa   4620 

tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga   4680 

aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat   4740 

gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg   4800 

ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt   4860 

agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt   4920 

cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat   4980 

ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt   5040 

ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt   5100 

attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct   5160 

ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt   5220 

ccgcgtggat cccggggtac cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat   5280 

gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt   5340 

aaaacacaac atatccagtc actatggcgg ccgcattagg caccccttag gaattcgctt   5400 

actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata   5460 

tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg cttctagaat gcagtttaag   5520 

gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt   5580 

attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat   5640 

aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg   5700 

accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc   5760 

caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat atagaattcc   5820 

tcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt   5880 

atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt   5940 

cttgtacaaa gtggtctcga gttaattaat tgatccgggt tattagtaca tttattaagc   6000 

gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt   6060 

aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt   6120 

atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc   6180 

aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc   6240 

cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt   6300 

gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc   6360 

actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aataaagctt ggacatattt   6420 

aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt   6480 

agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc   6540 

taacacgtcc gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg   6600 

tttttgggcg ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag   6660 

cgatggtgca ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg   6720 

agctgatgat aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg   6780 

cggaggtggt ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac   6840 

agtcggcacc tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag   6900 

acgagtgcga tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg   6960 

tgcagcgggt tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg   7020 

tggtggtggt ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc   7080 

cgccggtata atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg   7140 

cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa   7200 

ttcaatatta taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct   7260 

atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga   7320 

ttgtctcaag ctcggatccc gcacgccgat aacaagcctt ttcattttta ctacagcatt   7380 

gtagtggcga gacacttcgc tgtcgtcgac gtacatgtat gctttgttgt caaaaacgtc   7440 

gttggcaagc tttaaaatat ttaaaagaac atctctgttc agcaccactg tgttgtcgta   7500 

aatgttgttt ttgataattt gcgcttccgc agtatcgaca cgttcaaaaa attgatgcgc   7560 

atcaattttg ttgttcctat tattgaataa ataagattgt acagattcat atctacgatt   7620 

cgtcatggcc accacaaatg ctacgctgca aacgctggta caattttacg aaaactgcaa   7680 

aaacgtcaaa actcggtata aaataatcaa cgggcgcttt ggcaaaatat ctattttatc   7740 

gcacaagccc actagcaaat tgtatttgca gaaaacaatt tcggcgcaca attttaacgc   7800 

tgacgaaata aaagttcacc agttaatgag cgaccaccca aattttataa aaatctattt   7860 

taatcacggt tccatcaaca accaagtgat cgtgatggac tacattgact gtcccgattt   7920 

atttgaaaca ctacaaatta aaggcgagct ttcgtaccaa cttgttagca atattattag   7980 

acagctgtgt gaagcgctca acgatttgca caagcacaat ttcatacaca acgacataaa   8040 

actcgaaaat gtcttatatt tcgaagcact tgatcgcgtg tatgtttgcg attacggatt   8100 

gtgcaaacac gaaaactcac ttagcgtgca cgacggcacg ttggagtatt ttagtccgga   8160 

aaaaattcga cacacaacta tgcacgtttc gtttgactgg tacgccgtcg gcgtgttaac   8220 

atacaagttg ctaaccggcg gccgacaccc atttgaaaaa agcgaagacg aaatgttgga   8280 

cttgaatagc atgaagcgtc gtcagcaata caatgacatt ggcgttttaa aacacgttcg   8340 

taacgttaac gctcgtgact ttgtgtactg cctaacaaga tacaacatag attgtagact   8400 

cacaaattac aaacaaatta taaaacatga gtttttgtcg taaaaatgcc acttgtttta   8460 

cgagtagaat tctacgtgta acacacgatc taaaagatga tgtcattttt tatcaatgac   8520 

tcatttgttt taaaacagac ttgttttacg agtagaattc tacgtgtaaa gcatgatcgt   8580 

gagtggtgtt aataaaatca taaaaattat tgtaaatgtt tattatttaa aaacgattca   8640 

aatatataat aaaaacaatc tacatctatt tcttcacaat ccataacaca caacaggtcc   8700 

atcaatgagt ttttgtcttt atccgacata ctatgtgcat gtaacaaatc aaatacatct   8760 

tttaaatttt tatacacatc tttacattgt ctaccaaaat ctttaataac cctataacaa   8820 

ggaaaagact tttcttcttg cgtggttttg ccgcgcagat attgaaataa aatgtgcatg   8880 

cacgacaact tgtgtttact aaaatgctcc ttgcctatac cgcaaaaccg gccatacatt   8940 

tcggcgatta cacgcggaca attgtacgat tcgtctacgt gtaaacgatc atcataatca   9000 

ctcttgcgca aacgaataaa ttttttcacc gcttccgaca aacgaggcac caattcggcg   9060 

ggcacgcttc gatacattat tctgtgcaca taagttacca cacaaaattt attgtaccac   9120 

catccgacaa cgtcgttatt agggttgaac acgttggcga tgcgcagcag tttcccgttt   9180 

ctcatgaaat attcaaagcg gcccaaaata atttgcaagc aatccaacat gtcttgagaa   9240 

atttctcgtt caaaattgtt caaagagaat atctgccatc cgttttgaac gcgcacgctg   9300 

acgggaacca ccgcatcgat ttgctccaac acttcacgga cgttatcgtc gatgcccatc   9360 

gtttcgctgg tgctgaacca atgggaaagg ctcttgatgg aatcgcccgc gtctatcatc   9420 

ttgaccgctt cgtcaaaggt gcaactgccg ctcttcaaac gccgcatagc ggtcacgtcc   9480 

cgctctatgc acgacatacc gtttacgtac gattctgata ggtattcctg aactatacgg   9540 

taatggtgat acgactcgcc atacacgtcg tgcacctcat tgtatttagc ataataattg   9600 

taaattatta actttgcagc gagagacatg ttgtcagtaa agcggtgcta ggctcaataa   9660 

tactgatgta caggcacgcg tgctatttat atataatttc gcaaggaggg gagctgttat   9720 

cggttgctat tattaaagaa tggccgtctg tttttatcac aagcttggca gcctcaacca   9780 

tgaagcgtcg tcattgtaaa ttaaattctc tgcctcaaga attatttgac aagattgtcg   9840 

agtatttatc tttatctgat tactgcaatt tggtgcttgt ctgtaaaaga ccttctagta   9900 

aatataacgt gatatttgat agtactaatc accaacattt gaaaggcgtg tacaaaaaga   9960 

cagacgtgca aataacaagc tacaacgaat acatcaactg                        10000 

 
           
             4  
             10000  
             DNA  
             Artificial Sequence  
             
               Polyhedrin locus of vBacHTS_GST  
             
           
            4 

gaattctacc cgtaaagcga gtttagtttt gaaaaacaaa tgacatcatt tgtataatga     60 

catcatcccc tgattgtgtt ttacaagtag aattctatcc gtaaagcgag ttcagttttg    120 

aaaacaaatg agtcatacct aaacacgtta ataatcttct gatatcagct tatgactcaa    180 

gttatgagcc gtgtgcaaaa catgagataa gtttatgaca tcatccactg atcgtgcgtt    240 

acaagtagaa ttctactcgt aaagccagtt cggttatgag ccgtgtgcaa aacatgacat    300 

cagcttatga ctcatacttg attgtgtttt acgcgtagaa ttctactcgt aaagcgagtt    360 

cggttatgag ccgtgtgcaa aacatgacat cagcttatga gtcataatta atcgtgcgtt    420 

acaagtagaa ttctactcgt aaagcgagtt gaaggatcat atttagttgc gtttatgaga    480 

taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt tacaatgcgg    540 

ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc ccgagttgtt    600 

tgcgtacgtg actagcgaag aagatgtgtg gaccgcagaa cagatagtaa aacaaaaccc    660 

tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg gtgtgcattt    720 

tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt tgccgcctga    780 

aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt gtcccggcat    840 

gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg tgtgcagata    900 

tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg aaaaagccag    960 

aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat taatattatt   1020 

tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt ccagcgaacc   1080 

aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt gttccaatcg   1140 

acggtaggat taggccggat attctccacc acaatgttgg caacgttgat gttacgttta   1200 

tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt agtgccgtcg   1260 

cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac cgcgcgatcc   1320 

gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt ctgcattatt   1380 

tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag atcagtcatg   1440 

acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc aacgatgcgt   1500 

tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac cgcttgcagc   1560 

gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt gctctcctcc   1620 

tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg aattacttct   1680 

tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat aatcagctga   1740 

atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg gtcgcccctt   1800 

ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa taacgatttg   1860 

tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt taaactgtta   1920 

gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta ccgcaggttg   1980 

aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt gatacacgtg   2040 

tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt ggtaagcaat   2100 

aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg cagacggcgc   2160 

cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac atcgcaaaag   2220 

ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc ttatttaata   2280 

aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc gagcagttcg   2340 

ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac cagcggcgtg   2400 

ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg cacgtcggcc   2460 

tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt gcgcatatct   2520 

atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga aattaaatca   2580 

ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc gtcgattaaa   2640 

tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta ttcccgagtc   2700 

aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat ttaatgcaac   2760 

tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc cgttgtcgca   2820 

tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt gcgacgcaac   2880 

gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt tttacgaagc   2940 

gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga ctacaaaatt   3000 

accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt agtcgaatca   3060 

ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg tgtggagtcc   3120 

gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc ccgatgattt   3180 

tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg ttttggtcaa   3240 

aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta atgaaattaa   3300 

aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat gcgtagaagg   3360 

aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt gtataaaaaa   3420 

aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca ggaagaggtt   3480 

tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt   3540 

aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg aagtcatgca   3600 

tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt   3660 

cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt gtaattattg   3720 

aataataaaa caattataaa tgctaaattt gttttttatt aacgatacaa accaaacgca   3780 

acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc gctgaggtaa   3840 

tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat tttattttca   3900 

cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc atttttctcc   3960 

tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta tagagtaaat   4020 

tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc gctgcgcata   4080 

gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg tgttgcttta   4140 

attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg   4200 

gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac cggattaaca   4260 

taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc cttttctata   4320 

ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat gagacgcaca   4380 

aactaatatc acaaactgga aatgtctatc aatatatagt tgctgatatc atggagataa   4440 

ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt   4500 

aataaaaaaa cctataaata cggatct    atg tcc cct ata cta ggt tat tgg    4551 
                                 Met Ser Pro Ile Leu Gly Tyr Trp 
                                   1               5 

aaa att aag ggc ctt gtg caa ccc act cga ctt ctt ttg gaa tat ctt     4599 
Lys Ile Lys Gly Leu Val Gln Pro Thr Arg Leu Leu Leu Glu Tyr Leu 
     10                  15                  20 

gaa gaa aaa tat gaa gag cat ttg tat gag cgc gat gaa ggt gat aaa     4647 
Glu Glu Lys Tyr Glu Glu His Leu Tyr Glu Arg Asp Glu Gly Asp Lys 
 25                  30                  35                  40 

tgg cga aac aaa aag ttt gaa ttg ggt ttg gag ttt ccc aat ctt cct     4695 
Trp Arg Asn Lys Lys Phe Glu Leu Gly Leu Glu Phe Pro Asn Leu Pro 
                 45                  50                  55 

tat tat att gat ggt gat gtt aaa tta aca cag tct atg gcc atc ata     4743 
Tyr Tyr Ile Asp Gly Asp Val Lys Leu Thr Gln Ser Met Ala Ile Ile 
             60                  65                  70 

cgt tat ata gct gac aag cac aac atg ttg ggt ggt tgt cca aaa gag     4791 
Arg Tyr Ile Ala Asp Lys His Asn Met Leu Gly Gly Cys Pro Lys Glu 
         75                  80                  85 

cgt gca gag att tca atg ctt gaa gga gcg gtt ttg gat att aga tac     4839 
Arg Ala Glu Ile Ser Met Leu Glu Gly Ala Val Leu Asp Ile Arg Tyr 
     90                  95                 100 

ggt gtt tcg aga att gca tat agt aaa gac ttt gaa act ctc aaa gtt     4887 
Gly Val Ser Arg Ile Ala Tyr Ser Lys Asp Phe Glu Thr Leu Lys Val 
105                 110                 115                 120 

gat ttt ctt agc aag cta cct gaa atg ctg aaa atg ttc gaa gat cgt     4935 
Asp Phe Leu Ser Lys Leu Pro Glu Met Leu Lys Met Phe Glu Asp Arg 
                125                 130                 135 

tta tgt cat aaa aca tat tta aat ggt gat cat gta acc cat cct gac     4983 
Leu Cys His Lys Thr Tyr Leu Asn Gly Asp His Val Thr His Pro Asp 
            140                 145                 150 

ttc atg ttg tat gac gct ctt gat gtt gtt tta tac atg gac cca atg     5031 
Phe Met Leu Tyr Asp Ala Leu Asp Val Val Leu Tyr Met Asp Pro Met 
        155                 160                 165 

tgc ctg gat gcg ttc cca aaa tta gtt tgt ttt aaa aaa cgt att gaa     5079 
Cys Leu Asp Ala Phe Pro Lys Leu Val Cys Phe Lys Lys Arg Ile Glu 
    170                 175                 180 

gct atc cca caa att gat aag tac ttg aaa tcc agc aag tat ata gca     5127 
Ala Ile Pro Gln Ile Asp Lys Tyr Leu Lys Ser Ser Lys Tyr Ile Ala 
185                 190                 195                 200 

tgg cct ttg cag ggc tgg caa gcc acg ttt ggt ggt ggc gac cat cct     5175 
Trp Pro Leu Gln Gly Trp Gln Ala Thr Phe Gly Gly Gly Asp His Pro 
                205                 210                 215 

cca aaa     tcggatctg gttccgcgtg gatcccgggg taccacaagt ttgtacaaaa   5230 
Pro Lys 

aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata   5290 

aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt   5350 

aggcacccct taggaattcg cttactaaaa gccagataac agtatgcgta tttgcgcgct   5410 

gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt   5470 

gtgcttctag aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt   5530 

ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg   5590 

ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg   5650 

gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg   5710 

gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga   5770 

tgttctgggg aatatagaat tcctcaggtc gaccatagtg actggatatg ttgtgtttta   5830 

cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca   5890 

ttttacgttt ctcgttcagc tttcttgtac aaagtggtct cgagttaatt aattgatccg   5950 

ggttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt   6010 

tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa   6070 

atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg   6130 

taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta   6190 

tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct   6250 

ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta   6310 

tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg   6370 

ttaaataaag cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg   6430 

tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca   6490 

cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt   6550 

ggaattattt ctgattgcgg gcgtttttgg gcgggtttca atctaactgt gcccgatttt   6610 

aattcagaca acacgttaga aagcgatggt gcaggcggtg gtaacatttc agacggcaaa   6670 

tctactaatg gcggcggtgg tggagctgat gataaatcta ccatcggtgg aggcgcaggc   6730 

ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg cggtttaggc   6790 

tcaaatgtct ctttaggcaa cacagtcggc acctcaacta ttgtactggt ttcgggcgcc   6850 

gtttttggtt tgaccggtct gagacgagtg cgattttttt cgtttctaat agcttccaac   6910 

aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat tggtggagcg   6970 

ggcggcaatt cagacatcga tggtggtggt ggtggtggag gcgctggaat gttaggcacg   7030 

ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt ctggtttagt ttgttcgcgc   7090 

acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg tctgcttcga   7150 

ggcagcgctt ggggtggtgg caattcaata ttataattgg aatacaaatc gtaaaaatct   7210 

gctataagca ttgtaatttc gctatcgttt accgtgccga tatttaacaa ccgctcaatg   7270 

taagcaattg tattgtaaag agattgtctc aagctcggat cccgcacgcc gataacaagc   7330 

cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc gacgtacatg   7390 

tatgctttgt tgtcaaaaac gtcgttggca agctttaaaa tatttaaaag aacatctctg   7450 

ttcagcacca ctgtgttgtc gtaaatgttg tttttgataa tttgcgcttc cgcagtatcg   7510 

acacgttcaa aaaattgatg cgcatcaatt ttgttgttcc tattattgaa taaataagat   7570 

tgtacagatt catatctacg attcgtcatg gccaccacaa atgctacgct gcaaacgctg   7630 

gtacaatttt acgaaaactg caaaaacgtc aaaactcggt ataaaataat caacgggcgc   7690 

tttggcaaaa tatctatttt atcgcacaag cccactagca aattgtattt gcagaaaaca   7750 

atttcggcgc acaattttaa cgctgacgaa ataaaagttc accagttaat gagcgaccac   7810 

ccaaatttta taaaaatcta ttttaatcac ggttccatca acaaccaagt gatcgtgatg   7870 

gactacattg actgtcccga tttatttgaa acactacaaa ttaaaggcga gctttcgtac   7930 

caacttgtta gcaatattat tagacagctg tgtgaagcgc tcaacgattt gcacaagcac   7990 

aatttcatac acaacgacat aaaactcgaa aatgtcttat atttcgaagc acttgatcgc   8050 

gtgtatgttt gcgattacgg attgtgcaaa cacgaaaact cacttagcgt gcacgacggc   8110 

acgttggagt attttagtcc ggaaaaaatt cgacacacaa ctatgcacgt ttcgtttgac   8170 

tggtacgccg tcggcgtgtt aacatacaag ttgctaaccg gcggccgaca cccatttgaa   8230 

aaaagcgaag acgaaatgtt ggacttgaat agcatgaagc gtcgtcagca atacaatgac   8290 

attggcgttt taaaacacgt tcgtaacgtt aacgctcgtg actttgtgta ctgcctaaca   8350 

agatacaaca tagattgtag actcacaaat tacaaacaaa ttataaaaca tgagtttttg   8410 

tcgtaaaaat gccacttgtt ttacgagtag aattctacgt gtaacacacg atctaaaaga   8470 

tgatgtcatt ttttatcaat gactcatttg ttttaaaaca gacttgtttt acgagtagaa   8530 

ttctacgtgt aaagcatgat cgtgagtggt gttaataaaa tcataaaaat tattgtaaat   8590 

gtttattatt taaaaacgat tcaaatatat aataaaaaca atctacatct atttcttcac   8650 

aatccataac acacaacagg tccatcaatg agtttttgtc tttatccgac atactatgtg   8710 

catgtaacaa atcaaataca tcttttaaat ttttatacac atctttacat tgtctaccaa   8770 

aatctttaat aaccctataa caaggaaaag acttttcttc ttgcgtggtt ttgccgcgca   8830 

gatattgaaa taaaatgtgc atgcacgaca acttgtgttt actaaaatgc tccttgccta   8890 

taccgcaaaa ccggccatac atttcggcga ttacacgcgg acaattgtac gattcgtcta   8950 

cgtgtaaacg atcatcataa tcactcttgc gcaaacgaat aaattttttc accgcttccg   9010 

acaaacgagg caccaattcg gcgggcacgc ttcgatacat tattctgtgc acataagtta   9070 

ccacacaaaa tttattgtac caccatccga caacgtcgtt attagggttg aacacgttgg   9130 

cgatgcgcag cagtttcccg tttctcatga aatattcaaa gcggcccaaa ataatttgca   9190 

agcaatccaa catgtcttga gaaatttctc gttcaaaatt gttcaaagag aatatctgcc   9250 

atccgttttg aacgcgcacg ctgacgggaa ccaccgcatc gatttgctcc aacacttcac   9310 

ggacgttatc gtcgatgccc atcgtttcgc tggtgctgaa ccaatgggaa aggctcttga   9370 

tggaatcgcc cgcgtctatc atcttgaccg cttcgtcaaa ggtgcaactg ccgctcttca   9430 

aacgccgcat agcggtcacg tcccgctcta tgcacgacat accgtttacg tacgattctg   9490 

ataggtattc ctgaactata cggtaatggt gatacgactc gccatacacg tcgtgcacct   9550 

cattgtattt agcataataa ttgtaaatta ttaactttgc agcgagagac atgttgtcag   9610 

taaagcggtg ctaggctcaa taatactgat gtacaggcac gcgtgctatt tatatataat   9670 

ttcgcaagga ggggagctgt tatcggttgc tattattaaa gaatggccgt ctgtttttat   9730 

cacaagcttg gcagcctcaa ccatgaagcg tcgtcattgt aaattaaatt ctctgcctca   9790 

agaattattt gacaagattg tcgagtattt atctttatct gattactgca atttggtgct   9850 

tgtctgtaaa agaccttcta gtaaatataa cgtgatattt gatagtacta atcaccaaca   9910 

tttgaaaggc gtgtacaaaa agacagacgt gcaaataaca agctacaacg aatacatcaa   9970 

ctgtatttgc aacgaactga gacaagacga                                   10000 

 
           
             5  
             10000  
             DNA  
             Artificial Sequence  
             
               Polyhedrin locus of vBacHTS_GFP virus  
             
           
            5 

gaattctacc cgtaaagcga gtttagtttt gaaaaacaaa tgacatcatt tgtataatga     60 

catcatcccc tgattgtgtt ttacaagtag aattctatcc gtaaagcgag ttcagttttg    120 

aaaacaaatg agtcatacct aaacacgtta ataatcttct gatatcagct tatgactcaa    180 

gttatgagcc gtgtgcaaaa catgagataa gtttatgaca tcatccactg atcgtgcgtt    240 

acaagtagaa ttctactcgt aaagccagtt cggttatgag ccgtgtgcaa aacatgacat    300 

cagcttatga ctcatacttg attgtgtttt acgcgtagaa ttctactcgt aaagcgagtt    360 

cggttatgag ccgtgtgcaa aacatgacat cagcttatga gtcataatta atcgtgcgtt    420 

acaagtagaa ttctactcgt aaagcgagtt gaaggatcat atttagttgc gtttatgaga    480 

taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt tacaatgcgg    540 

ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc ccgagttgtt    600 

tgcgtacgtg actagcgaag aagatgtgtg gaccgcagaa cagatagtaa aacaaaaccc    660 

tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg gtgtgcattt    720 

tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt tgccgcctga    780 

aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt gtcccggcat    840 

gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg tgtgcagata    900 

tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg aaaaagccag    960 

aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat taatattatt   1020 

tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt ccagcgaacc   1080 

aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt gttccaatcg   1140 

acggtaggat taggccggat attctccacc acaatgttgg caacgttgat gttacgttta   1200 

tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt agtgccgtcg   1260 

cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac cgcgcgatcc   1320 

gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt ctgcattatt   1380 

tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag atcagtcatg   1440 

acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc aacgatgcgt   1500 

tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac cgcttgcagc   1560 

gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt gctctcctcc   1620 

tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg aattacttct   1680 

tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat aatcagctga   1740 

atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg gtcgcccctt   1800 

ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa taacgatttg   1860 

tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt taaactgtta   1920 

gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta ccgcaggttg   1980 

aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt gatacacgtg   2040 

tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt ggtaagcaat   2100 

aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg cagacggcgc   2160 

cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac atcgcaaaag   2220 

ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc ttatttaata   2280 

aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc gagcagttcg   2340 

ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac cagcggcgtg   2400 

ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg cacgtcggcc   2460 

tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt gcgcatatct   2520 

atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga aattaaatca   2580 

ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc gtcgattaaa   2640 

tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta ttcccgagtc   2700 

aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat ttaatgcaac   2760 

tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc cgttgtcgca   2820 

tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt gcgacgcaac   2880 

gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt tttacgaagc   2940 

gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga ctacaaaatt   3000 

accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt agtcgaatca   3060 

ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg tgtggagtcc   3120 

gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc ccgatgattt   3180 

tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg ttttggtcaa   3240 

aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta atgaaattaa   3300 

aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat gcgtagaagg   3360 

aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt gtataaaaaa   3420 

aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca ggaagaggtt   3480 

tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt   3540 

aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg aagtcatgca   3600 

tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt   3660 

cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt gtaattattg   3720 

aataataaaa caattataaa tgctaaattt gttttttatt aacgatacaa accaaacgca   3780 

acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc gctgaggtaa   3840 

tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat tttattttca   3900 

cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc atttttctcc   3960 

tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta tagagtaaat   4020 

tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc gctgcgcata   4080 

gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg tgttgcttta   4140 

attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg   4200 

gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac cggattaaca   4260 

taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc cttttctata   4320 

ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat gagacgcaca   4380 

aactaatatc acaaactgga aatgtctatc aatatatagt tgctgatatc atggagataa   4440 

ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt   4500 

aataaaaaaa cctataaata cggatccacc atg gtg agc aag ggc gag gag ctg    4554 
                                 Met Val Ser Lys Gly Glu Glu Leu 
                                   1               5 

ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac     4602 
Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
     10                  15                  20 

ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac     4650 
Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 
 25                  30                  35                  40 

ggc aag ctg acc ctg aag ttc atc tgc acc acc ggc aag ctg ccc gtg     4698 
Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
                 45                  50                  55 

ccc tgg ccc acc ctc gtg acc acc ctg acc tac ggc gtg cag tgc ttc     4746 
Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe 
             60                  65                  70 

agc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc     4794 
Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
         75                  80                  85 

atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac     4842 
Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
     90                  95                 100 

ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg     4890 
Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
105                 110                 115                 120 

gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac     4938 
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
                125                 130                 135 

atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat     4986 
Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
            140                 145                 150 

atc atg gcc gac aag cag aag aac ggc atc aag gtg aac ttc aag atc     5034 
Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile 
        155                 160                 165 

cgc cac aac atc gag gac ggc agc gtg cag ctc gcc gac cac tac cag     5082 
Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
    170                 175                 180 

cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac     5130 
Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
185                 190                 195                 200 

tac ctg agc acc cag tcc gcc ctg agc aaa gac ccc aac gag aag cgc     5178 
Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 
                205                 210                 215 

gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc     5226 
Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
            220                 225                 230 

ggc atg gac gag ctg tac aag  ggt accacaagtt tgtacaaaaa agctgaacga   5280 
Gly Met Asp Glu Leu Tyr Lys 
        235 

gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact   5340 

acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcacccctt   5400 

aggaattcgc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg   5460 

tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggtg tgcttctaga   5520 

atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta   5580 

cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt   5640 

ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc   5700 

tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg   5760 

gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga   5820 

atatagaatt cctcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt   5880 

agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc   5940 

tcgttcagct ttcttgtaca aagtggtctc gagttaatta attgatccgg gttattagta   6000 

catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt   6060 

taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt   6120 

ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt   6180 

tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt   6240 

ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt   6300 

cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt   6360 

atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc   6420 

ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca   6480 

accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt   6540 

aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc   6600 

tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa   6660 

cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg   6720 

cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg   6780 

cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc   6840 

tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg tttttggttt   6900 

gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca attgttgtct   6960 

gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc   7020 

agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg   7080 

tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca cgattgtggg   7140 

caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg   7200 

gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg ctataagcat   7260 

tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt aagcaattgt   7320 

attgtaaaga gattgtctca agctcggatc ccgcacgccg ataacaagcc ttttcatttt   7380 

tactacagca ttgtagtggc gagacacttc gctgtcgtcg acgtacatgt atgctttgtt   7440 

gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga acatctctgt tcagcaccac   7500 

tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc gcagtatcga cacgttcaaa   7560 

aaattgatgc gcatcaattt tgttgttcct attattgaat aaataagatt gtacagattc   7620 

atatctacga ttcgtcatgg ccaccacaaa tgctacgctg caaacgctgg tacaatttta   7680 

cgaaaactgc aaaaacgtca aaactcggta taaaataatc aacgggcgct ttggcaaaat   7740 

atctatttta tcgcacaagc ccactagcaa attgtatttg cagaaaacaa tttcggcgca   7800 

caattttaac gctgacgaaa taaaagttca ccagttaatg agcgaccacc caaattttat   7860 

aaaaatctat tttaatcacg gttccatcaa caaccaagtg atcgtgatgg actacattga   7920 

ctgtcccgat ttatttgaaa cactacaaat taaaggcgag ctttcgtacc aacttgttag   7980 

caatattatt agacagctgt gtgaagcgct caacgatttg cacaagcaca atttcataca   8040 

caacgacata aaactcgaaa atgtcttata tttcgaagca cttgatcgcg tgtatgtttg   8100 

cgattacgga ttgtgcaaac acgaaaactc acttagcgtg cacgacggca cgttggagta   8160 

ttttagtccg gaaaaaattc gacacacaac tatgcacgtt tcgtttgact ggtacgccgt   8220 

cggcgtgtta acatacaagt tgctaaccgg cggccgacac ccatttgaaa aaagcgaaga   8280 

cgaaatgttg gacttgaata gcatgaagcg tcgtcagcaa tacaatgaca ttggcgtttt   8340 

aaaacacgtt cgtaacgtta acgctcgtga ctttgtgtac tgcctaacaa gatacaacat   8400 

agattgtaga ctcacaaatt acaaacaaat tataaaacat gagtttttgt cgtaaaaatg   8460 

ccacttgttt tacgagtaga attctacgtg taacacacga tctaaaagat gatgtcattt   8520 

tttatcaatg actcatttgt tttaaaacag acttgtttta cgagtagaat tctacgtgta   8580 

aagcatgatc gtgagtggtg ttaataaaat cataaaaatt attgtaaatg tttattattt   8640 

aaaaacgatt caaatatata ataaaaacaa tctacatcta tttcttcaca atccataaca   8700 

cacaacaggt ccatcaatga gtttttgtct ttatccgaca tactatgtgc atgtaacaaa   8760 

tcaaatacat cttttaaatt tttatacaca tctttacatt gtctaccaaa atctttaata   8820 

accctataac aaggaaaaga cttttcttct tgcgtggttt tgccgcgcag atattgaaat   8880 

aaaatgtgca tgcacgacaa cttgtgttta ctaaaatgct ccttgcctat accgcaaaac   8940 

cggccataca tttcggcgat tacacgcgga caattgtacg attcgtctac gtgtaaacga   9000 

tcatcataat cactcttgcg caaacgaata aattttttca ccgcttccga caaacgaggc   9060 

accaattcgg cgggcacgct tcgatacatt attctgtgca cataagttac cacacaaaat   9120 

ttattgtacc accatccgac aacgtcgtta ttagggttga acacgttggc gatgcgcagc   9180 

agtttcccgt ttctcatgaa atattcaaag cggcccaaaa taatttgcaa gcaatccaac   9240 

atgtcttgag aaatttctcg ttcaaaattg ttcaaagaga atatctgcca tccgttttga   9300 

acgcgcacgc tgacgggaac caccgcatcg atttgctcca acacttcacg gacgttatcg   9360 

tcgatgccca tcgtttcgct ggtgctgaac caatgggaaa ggctcttgat ggaatcgccc   9420 

gcgtctatca tcttgaccgc ttcgtcaaag gtgcaactgc cgctcttcaa acgccgcata   9480 

gcggtcacgt cccgctctat gcacgacata ccgtttacgt acgattctga taggtattcc   9540 

tgaactatac ggtaatggtg atacgactcg ccatacacgt cgtgcacctc attgtattta   9600 

gcataataat tgtaaattat taactttgca gcgagagaca tgttgtcagt aaagcggtgc   9660 

taggctcaat aatactgatg tacaggcacg cgtgctattt atatataatt tcgcaaggag   9720 

gggagctgtt atcggttgct attattaaag aatggccgtc tgtttttatc acaagcttgg   9780 

cagcctcaac catgaagcgt cgtcattgta aattaaattc tctgcctcaa gaattatttg   9840 

acaagattgt cgagtattta tctttatctg attactgcaa tttggtgctt gtctgtaaaa   9900 

gaccttctag taaatataac gtgatatttg atagtactaa tcaccaacat ttgaaaggcg   9960 

tgtacaaaaa gacagacgtg caaataacaa gctacaacga                        10000 

 
           
             6  
             21  
             DNA  
             Artificial Sequence  
             
               Baculo-Forward primer  
             
           
            6 

actgttttcg taacagtttt g                                               21 

 
           
             7  
             19  
             DNA  
             Artificial Sequence  
             
               Baculo-Reverse primer  
             
           
            7 

acaacgcaca gaatctagc                                                  19 

 
           
             8  
             14000  
             DNA  
             Artificial Sequence  
             
               Polyhedrin locus of vBacHTS2  
             
           
            8 

gaattctacc cgtaaagcga gtttagtttt gaaaaacaaa tgacatcatt tgtataatga     60 

catcatcccc tgattgtgtt ttacaagtag aattctatcc gtaaagcgag ttcagttttg    120 

aaaacaaatg agtcatacct aaacacgtta ataatcttct gatatcagct tatgactcaa    180 

gttatgagcc gtgtgcaaaa catgagataa gtttatgaca tcatccactg atcgtgcgtt    240 

acaagtagaa ttctactcgt aaagccagtt cggttatgag ccgtgtgcaa aacatgacat    300 

cagcttatga ctcatacttg attgtgtttt acgcgtagaa ttctactcgt aaagcgagtt    360 

cggttatgag ccgtgtgcaa aacatgacat cagcttatga gtcataatta atcgtgcgtt    420 

acaagtagaa ttctactcgt aaagcgagtt gaaggatcat atttagttgc gtttatgaga    480 

taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt tacaatgcgg    540 

ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc ccgagttgtt    600 

tgcgtacgtg actagcgaag aagatgtgtg gaccgcagaa cagatagtaa aacaaaaccc    660 

tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg gtgtgcattt    720 

tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt tgccgcctga    780 

aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt gtcccggcat    840 

gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg tgtgcagata    900 

tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg aaaaagccag    960 

aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat taatattatt   1020 

tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt ccagcgaacc   1080 

aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt gttccaatcg   1140 

acggtaggat taggccggat attctccacc acaatgttgg caacgttgat gttacgttta   1200 

tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt agtgccgtcg   1260 

cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac cgcgcgatcc   1320 

gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt ctgcattatt   1380 

tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag atcagtcatg   1440 

acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc aacgatgcgt   1500 

tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac cgcttgcagc   1560 

gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt gctctcctcc   1620 

tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg aattacttct   1680 

tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat aatcagctga   1740 

atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg gtcgcccctt   1800 

ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa taacgatttg   1860 

tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt taaactgtta   1920 

gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta ccgcaggttg   1980 

aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt gatacacgtg   2040 

tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt ggtaagcaat   2100 

aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg cagacggcgc   2160 

cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac atcgcaaaag   2220 

ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc ttatttaata   2280 

aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc gagcagttcg   2340 

ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac cagcggcgtg   2400 

ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg cacgtcggcc   2460 

tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt gcgcatatct   2520 

atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga aattaaatca   2580 

ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc gtcgattaaa   2640 

tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta ttcccgagtc   2700 

aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat ttaatgcaac   2760 

tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc cgttgtcgca   2820 

tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt gcgacgcaac   2880 

gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt tttacgaagc   2940 

gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga ctacaaaatt   3000 

accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt agtcgaatca   3060 

ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg tgtggagtcc   3120 

gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc ccgatgattt   3180 

tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg ttttggtcaa   3240 

aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta atgaaattaa   3300 

aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat gcgtagaagg   3360 

aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt gtataaaaaa   3420 

aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca ggaagaggtt   3480 

tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt   3540 

aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg aagtcatgca   3600 

tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt   3660 

cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt gtaattattg   3720 

aataataaaa caattataaa tgctaaattt gttttttatt aacgatacaa accaaacgca   3780 

acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc gctgaggtaa   3840 

tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat tttattttca   3900 

cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc atttttctcc   3960 

tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta tagagtaaat   4020 

tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc gctgcgcata   4080 

gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg tgttgcttta   4140 

attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg   4200 

gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac cggattaaca   4260 

taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc cttttctata   4320 

ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat gagacgcaca   4380 

aactaatatc acaaactgga aatgtctatc aatatatagt tgctgatatc atggagataa   4440 

ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt   4500 

aataaaaaaa cctataaata cggatcccgg ggtaccacaa gtttgtacaa aaaagctgaa   4560 

cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag   4620 

actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc   4680 

cttaggtagg gtcaccgtcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg   4740 

ccggcacggc ctgggtaacc aggtattttg tccacataac cgtgcgcaaa atgttgtgga   4800 

taagcaggac acagcagcaa tccacagcag gcatacaacc gcacaccgag gttactccgt   4860 

tctacaggtt acgacgacat gtcaatactt gcccttgaca ggcattgatg gaatcgtagt   4920 

ctcacgctga tagtctgatc gacaatacaa gtgggaccgt ggtcccagac cgataatcag   4980 

accgacaaca cgagtgggat cgtggtccca gactaataat cagaccgacg atacgagtgg   5040 

gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gttccagact   5100 

aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc agaccgacga   5160 

tacgagtggg accatggtcc cagactaata atcagaccga cgatacgagt gggaccgtgg   5220 

tcccagtctg attatcagac cgacgatacg agtgggaccg tggtcccaga ctaataatca   5280 

gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg   5340 

ggaccgtggt cccagtctga ttatcagacc gacgatacaa gtggaacagt gggcccagag   5400 

agaatattca ggccagttat gctttctggc ctgtaacaaa ggacattaag taaagacaga   5460 

taaacgtaga ctaaaacgtg gtcgcatcag ggtgctggct tttcaagttc cttaagaatg   5520 

gcctcaattt tctctataca ctcagttgga acacgagacc tgtccaggtt aagcaccatt   5580 

ttatcgccct tatacaatac tgtcgctcca ggagcaaact gatgtcgtga gcttaaacta   5640 

gttcttgatg cagatgacgt tttaagcaca gaagttaaaa gagtgataac ttcttcagct   5700 

tcaaatatca ccccagcttt tttctgctca tgaaggttag atgcctgctg cttaagtaat   5760 

tcctctttat ctgtaaaggc tttttgaagt gcatcacctg accgggcaga tagttcaccg   5820 

gggtgagaaa aaagagcaac aactgattta ggcaatttgg cggtgttgat acagcgggta   5880 

ataatcttac gtgaaatatt ttccgcatca gccagcgcag aaatatttcc agcaaattca   5940 

ttctgcaatc ggcttgcata acgctgacca cgttcataag cacttgttgg gcgataatcg   6000 

ttacccaatc tggataatgc agccatctgc tcatcatcca gctcgccaac cagaacacga   6060 

taatcacttt cggtaagtgc agcagcttta cgacggcgac tcccatcggc aatttctatg   6120 

acaccagata ctcttcgacc gaacgccggt gtctgttgac cagtcagtag aaaagaaggg   6180 

atgagatcat ccagtgcgtc ctcagtaagc agctcctggt cacgttcatt acctgaccat   6240 

acccgagagg tcttctcaac actatcaccc cggagcactt caagagtaaa cttcacatcc   6300 

cgaccacata caggcaaagt aatggcatta ccgcgagcca ttactcctac gcgcgcaatt   6360 

aacgaatcca ccatcggggc agctggtgtc gataacgaag tatcttcaac cggttgagta   6420 

ttgagcgtat gttttggaat aacaggcgca cgcttcatta tctaatctcc cagcgtggtt   6480 

taatcagacg atcgaaaatt tcattgcaga caggttccca aatagaaaga gcatttctcc   6540 

aggcaccagt tgaagagcgt tgatcaatgg cctgttcaaa aacagttctc atccggatct   6600 

gacctttacc aacttcatcc gtttcacgta caacattttt tagaaccatg cttccccagg   6660 

catcccgaat ttgctcctcc atccacgggg actgagagcc attactattg ctgtatttgg   6720 

taagcaaaat acgtacatca ggctcgaacc ctttaagatc aacgttcttg agcagatcac   6780 

gaagcatatc gaaaaactgc agtgcggagg tgtagtcaaa caactcagca ggcgtgggaa   6840 

caatcagcac atcagcagca catacgacat taatcgtgcc gatacccagg ttaggcgcgc   6900 

tgtcaataac tatgacatca tagtcatgag caacagtttc aatggccagt cggagcatca   6960 

ggtgtggatc ggtgggcagt ttaccttcat caaatttgcc cattaactca gtttcaatac   7020 

ggtgcagagc cagacaggaa ggaataatgt caagccccgg ccagcaagtg ggctttattg   7080 

cataagtgac atcgtccttt tccccaagat agaaaggcag gagagtgtct tctgcatgaa   7140 

tatgaagatc tggtacccat ccgtgataca ttgaggctgt tccctggggg tcgttacctt   7200 

ccacgagcaa aacacgtagc cccttcagag ccagatcctg agcaagatga acagaaactg   7260 

aggttttgta aacgccacct ttatgggcag caaccccgat caccggtgga aatacgtctt   7320 

cagcacgtcg caatcgcgta ccaaacacat cacgcatatg attaatttgt tcaattgtat   7380 

aaccaacacg ttgctcaacc cgtcctcgaa tttccatatc cgggtgcggt agtcgccctg   7440 

ctttctcggc atctctgata gcctgagaag aaaccccaac taaatccgct gcttcaccta   7500 

ttctccagcg ccgggttatt ttcctcgctt ccgggctgtc atcattaaac tgtgcaatgg   7560 

cgatagcctt cgtcatttca tgaccagcgt ttatgcactg gttaagtgtt tccatgagtt   7620 

tcattctgaa catcctttaa tcattgcttt gcgttttttt attaaatctt gcaatttact   7680 

gcaaagcaac aacaaaatcg caaagtcatc aaaaaaccgc aaagttgttt aaaataagag   7740 

caacactaca aaaggagata agaagagcac atacctcagt cacttattat cactagcgct   7800 

cgccgcagcc gtgtaaccga gcatagcgag cgaactggcg aggaagcaaa gaagaactgt   7860 

tctgtcagat agctcttacg ctcagcgcaa gaagaaatat ccaccgtggg aaaaactcca   7920 

ggtagaggta cacacgcgga tagccaattc agagtaataa actgtgataa tcaaccctca   7980 

tcaatgatga cgaactaacc cccgatatca ggtcacatga cgaagggaaa gagaaggaaa   8040 

tcaactgtga caaactgccc tcaaatttgg cttccttaaa aattacagtt caaaaagtat   8100 

gagaaaatcc atgcaggctg aaggaaacag caaaactgtg acaaattacc ctcagtaggt   8160 

cagaacaaat gtgacgaacc accctcaaat ctgtgacaga taaccctcag actatcctgt   8220 

cgtcatggaa gtgatatcgc ggaaggaaaa tacgatatga gtcgtctggc ggcctttctt   8280 

tttctcaatg tatgagaggc gcattggagt tctgctgttg atctcattaa cacagacctg   8340 

caggaagcgg cggcggaagt caggcatacg ctggtaactt tgaggcagct ggtaacgctc   8400 

tatgatccag tcgattttca gagagacgat gcctgagcca tccggcttac gatactgaca   8460 

cagggattcg tataaacgca tggcatacgg attggtgatt tcttttgttt cactaagccg   8520 

aaactgcgta aaccggttct gtaacccgat aaagaaggga atgagatatg ggttgatatg   8580 

tacactgtaa agccctctgg atggactgtg cgcacgtttg ataaaccaag gaaaagattc   8640 

atagcctttt tcatcgccgg catcctcttc agggcgataa aaaaccactt ccttccccgc   8700 

gaaactcttc aatgcctgcc gtatatcctt actggcttcc gcagaggtca atccgaatat   8760 

ttcagcatat ttagcaacat ggatctcgca gataccgtca tgttcctgta gggtgccatc   8820 

agattttctg atctggtcaa cgaacagata cagcatacgt ttttgatccc gggagagact   8880 

atatgccgcc tcagtgaggt cgtttgactg gacgattcgc gggctatttt tacgtttctt   8940 

gtgattgata accgctgttt ccgccatgac agatccatgt gaagtgtgac aagtttttag   9000 

attgtcacac taaataaaaa agagtcaata agcagggata actttgtgaa aaaacagctt   9060 

cttctgaggg caatttgtca cagggttaag ggcaatttgt cacagacagg actgtcattt   9120 

gagggtgatt tgtcacactg aaagggcaat ttgtcacaac accttctcta gaaccagcat   9180 

ggataaaggc ctacaaggcg ctctaaaaaa gaagatctaa aaactataaa aaaaataatt   9240 

ataaaaatat ccccgtggat aagtggataa ccccaaggga agttttttca ggcatcgtgt   9300 

gtaagcagaa tatataagtg ctgttccctg gtgcttcctc gctcactcga gggcttcgcc   9360 

ctgtcgctca actgcggcga gcactactgg ctgtaaaagg acagaccaca tcatggttct   9420 

gtgttcatta ggttgttctg tccattgctg acataatccg ctccacttca acgtaacacc   9480 

gcacgaagat ttctattgtt cctgaaggca tattcaaatc gttttcgtta ccgcttgcag   9540 

gcatcatgac agaacactac ttcctataaa cgctacacag gctcctgaga ttaataatgc   9600 

ggatctctac gataatggga gattttcccg actgtttcgt tcgcttctca gtggataaca   9660 

gccagcttct ctgtttaaca gacaaaaaca gcatatccac tcagttccac atttccatat   9720 

aaaggccaag gcatttattc tcaggataat tgtttcagca tcgcaaccgc atcagactcc   9780 

ggcatcgcaa actgcacccg gtgccgggca gccacatcca gcgcaaaaac cttcgtgtag   9840 

acttccgttg aactgatgga cttatgtccc atcaggcttt gcagaacttt cagcggtata   9900 

ccggcataca gcatgtgcat cgcataggaa tggcggaacg tatgtggtgt gaccggaaca   9960 

gagaacgtca caccgtcagc agcagcggcg gcaaccgcct ccccaatcca ggtcctgacc  10020 

gttctgtccg tcacttccca gatccgcgct ttctctgtcc ttcctgtgcg acggttacgc  10080 

cgctccatga gcttatcgcg aataaatacc tgtgacggaa gatcacttcg cagaataaat  10140 

aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc gaaaatgaga  10200 

cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg  10260 

cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat  10320 

cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt  10380 

tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt  10440 

aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg  10500 

cctgatgaat gctcatccgg agttccgtat ggcaatgaaa gacggtgagc tggtgatatg  10560 

ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct  10620 

ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc  10680 

gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt  10740 

ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa  10800 

cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat  10860 

gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct  10920 

taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag  10980 

ttattggtgc ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa  11040 

tggcagaaat tcgatgataa gctgtcaaac atgagaattg gtcgacggcg cgccaaagct  11100 

tgcatgcctg cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc  11160 

cctgcgtaag cggggcacat ttcattacct ctttctccgc acccgaccct caggtcgacc  11220 

atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct  11280 

aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag  11340 

tggtctcgag ttaattaatt gatccgggtt attagtacat ttattaagcg ctagattctg  11400 

tgcgttgttg atttacagac aattgttgta cgtattttaa taattcatta aatttataat  11460 

ctttagggtg gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt  11520 

taaatattaa atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt  11580 

gtttttccga accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg  11640 

ccaaatcttg tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata  11700 

aaggttcgac gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag  11760 

tgtacaattg actcgacgta aacacgttaa ataaagcttg gacatattta acatcgggcg  11820 

tgttagcttt attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt  11880 

ccgaagacga ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg  11940 

cgatcaaatt tgtagttgag ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg  12000 

gtttcaatct aactgtgccc gattttaatt cagacaacac gttagaaagc gatggtgcag  12060 

gcggtggtaa catttcagac ggcaaatcta ctaatggcgg cggtggtgga gctgatgata  12120 

aatctaccat cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg  12180 

gcggtgatgc agacggcggt ttaggctcaa atgtctcttt aggcaacaca gtcggcacct  12240 

caactattgt actggtttcg ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat  12300 

ttttttcgtt tctaatagct tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt  12360 

gaggttccgt cggcattggt ggagcgggcg gcaattcaga catcgatggt ggtggtggtg  12420 

gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc gccggtataa  12480 

tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct  12540 

gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat tcaatattat  12600 

aattggaata caaatcgtaa aaatctgcta taagcattgt aatttcgcta tcgtttaccg  12660 

tgccgatatt taacaaccgc tcaatgtaag caattgtatt gtaaagagat tgtctcaagc  12720 

tcggatcccg cacgccgata acaagccttt tcatttttac tacagcattg tagtggcgag  12780 

acacttcgct gtcgtcgacg tacatgtatg ctttgttgtc aaaaacgtcg ttggcaagct  12840 

ttaaaatatt taaaagaaca tctctgttca gcaccactgt gttgtcgtaa atgttgtttt  12900 

tgataatttg cgcttccgca gtatcgacac gttcaaaaaa ttgatgcgca tcaattttgt  12960 

tgttcctatt attgaataaa taagattgta cagattcata tctacgattc gtcatggcca  13020 

ccacaaatgc tacgctgcaa acgctggtac aattttacga aaactgcaaa aacgtcaaaa  13080 

ctcggtataa aataatcaac gggcgctttg gcaaaatatc tattttatcg cacaagccca  13140 

ctagcaaatt gtatttgcag aaaacaattt cggcgcacaa ttttaacgct gacgaaataa  13200 

aagttcacca gttaatgagc gaccacccaa attttataaa aatctatttt aatcacggtt  13260 

ccatcaacaa ccaagtgatc gtgatggact acattgactg tcccgattta tttgaaacac  13320 

tacaaattaa aggcgagctt tcgtaccaac ttgttagcaa tattattaga cagctgtgtg  13380 

aagcgctcaa cgatttgcac aagcacaatt tcatacacaa cgacataaaa ctcgaaaatg  13440 

tcttatattt cgaagcactt gatcgcgtgt atgtttgcga ttacggattg tgcaaacacg  13500 

aaaactcact tagcgtgcac gacggcacgt tggagtattt tagtccggaa aaaattcgac  13560 

acacaactat gcacgtttcg tttgactggt acgccgtcgg cgtgttaaca tacaagttgc  13620 

taaccggcgg ccgacaccca tttgaaaaaa gcgaagacga aatgttggac ttgaatagca  13680 

tgaagcgtcg tcagcaatac aatgacattg gcgttttaaa acacgttcgt aacgttaacg  13740 

ctcgtgactt tgtgtactgc ctaacaagat acaacataga ttgtagactc acaaattaca  13800 

aacaaattat aaaacatgag tttttgtcgt aaaaatgcca cttgttttac gagtagaatt  13860 

ctacgtgtaa cacacgatct aaaagatgat gtcatttttt atcaatgact catttgtttt  13920 

aaacagact tgttttacga gtagaattct acgtgtaaag catgatcgtg agtggtgtta   13980 

taaaatcat aaaaattatt                                               14000 

 
           
             9  
             13100  
             DNA  
             Artificial Sequence  
             
               Polyhedrin locus of vBacHTS2_GFP  
             
           
            9 

gaattctacc cgtaaagcga gtttagtttt gaaaaacaaa tgacatcatt tgtataatga     60 

catcatcccc tgattgtgtt ttacaagtag aattctatcc gtaaagcgag ttcagttttg    120 

aaaacaaatg agtcatacct aaacacgtta ataatcttct gatatcagct tatgactcaa    180 

gttatgagcc gtgtgcaaaa catgagataa gtttatgaca tcatccactg atcgtgcgtt    240 

acaagtagaa ttctactcgt aaagccagtt cggttatgag ccgtgtgcaa aacatgacat    300 

cagcttatga ctcatacttg attgtgtttt acgcgtagaa ttctactcgt aaagcgagtt    360 

cggttatgag ccgtgtgcaa aacatgacat cagcttatga gtcataatta atcgtgcgtt    420 

acaagtagaa ttctactcgt aaagcgagtt gaaggatcat atttagttgc gtttatgaga    480 

taagattgaa agcacgtgta aaatgtttcc cgcgcgttgg cacaactatt tacaatgcgg    540 

ccaagttata aaagattcta atctgatatg ttttaaaaca cctttgcggc ccgagttgtt    600 

tgcgtacgtg actagcgaag aagatgtgtg gaccgcagaa cagatagtaa aacaaaaccc    660 

tagtattgga gcaataatcg atttaaccaa cacgtctaaa tattatgatg gtgtgcattt    720 

tttgcgggcg ggcctgttat acaaaaaaat tcaagtacct ggccagactt tgccgcctga    780 

aagcatagtt caagaattta ttgacacggt aaaagaattt acagaaaagt gtcccggcat    840 

gttggtgggc gtgcactgca cacacggtat taatcgcacc ggttacatgg tgtgcagata    900 

tttaatgcac accctgggta ttgcgccgca ggaagccata gatagattcg aaaaagccag    960 

aggtcacaaa attgaaagac aaaattacgt tcaagattta ttaatttaat taatattatt   1020 

tgcattcttt aacaaatact ttatcctatt ttcaaattgt tgcgcttctt ccagcgaacc   1080 

aaaactatgc ttcgcttgct ccgtttagct tgtagccgat cagtggcgtt gttccaatcg   1140 

acggtaggat taggccggat attctccacc acaatgttgg caacgttgat gttacgttta   1200 

tgcttttggt tttccacgta cgtcttttgg ccggtaatag ccgtaaacgt agtgccgtcg   1260 

cgcgtcacgc acaacaccgg atgtttgcgc ttgtccgcgg ggtattgaac cgcgcgatcc   1320 

gacaaatcca ccactttggc aactaaatcg gtgacctgcg cgtctttttt ctgcattatt   1380 

tcgtctttct tttgcatggt ttcctggaag ccggtgtaca tgcggtttag atcagtcatg   1440 

acgcgcgtga cctgcaaatc tttggcctcg atctgcttgt ccttgatggc aacgatgcgt   1500 

tcaataaact cttgtttttt aacaagttcc tcggtttttt gcgccaccac cgcttgcagc   1560 

gcgtttgtgt gctcggtgaa tgtcgcaatc agcttagtca ccaactgttt gctctcctcc   1620 

tcccgttgtt tgatcgcggg atcgtacttg ccggtgcaga gcacttgagg aattacttct   1680 

tctaaaagcc attcttgtaa ttctatggcg taaggcaatt tggacttcat aatcagctga   1740 

atcacgccgg atttagtaat gagcactgta tgcggctgca aatacagcgg gtcgcccctt   1800 

ttcacgacgc tgttagaggt agggccccca ttttggatgg tctgctcaaa taacgatttg   1860 

tatttattgt ctacatgaac acgtatagct ttatcacaaa ctgtatattt taaactgtta   1920 

gcgacgtcct tggccacgaa ccggacctgt tggtcgcgct ctagcacgta ccgcaggttg   1980 

aacgtatctt ctccaaattt aaattctcca attttaacgc gagccatttt gatacacgtg   2040 

tgtcgatttt gcaacaacta ttgtttttta acgcaaacta aacttattgt ggtaagcaat   2100 

aattaaatat gggggaacat gcgccgctac aacactcgtc gttatgaacg cagacggcgc   2160 

cggtctcggc gcaagcggct aaaacgtgtt gcgcgttcaa cgcggcaaac atcgcaaaag   2220 

ccaatagtac agttttgatt tgcatattaa cggcgatttt ttaaattatc ttatttaata   2280 

aatagttatg acgcctacaa ctccccgccc gcgttgactc gctgcacctc gagcagttcg   2340 

ttgacgcctt cctccgtgtg gccgaacacg tcgagcgggt ggtcgatgac cagcggcgtg   2400 

ccgcacgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg cacgtcggcc   2460 

tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt gcgcatatct   2520 

atcgtggcgt tgggcatgta cgtccgaacg ttgatttgca tgcaagccga aattaaatca   2580 

ttgcgattag tgcgattaaa acgttgtaca tcctcgcttt taatcatgcc gtcgattaaa   2640 

tcgcgcaatc gagtcaagtg atcaaagtgt ggaataatgt tttctttgta ttcccgagtc   2700 

aagcgcagcg cgtattttaa caaactagcc atcttgtaag ttagtttcat ttaatgcaac   2760 

tttatccaat aatatattat gtatcgcacg tcaagaatta acaatgcgcc cgttgtcgca   2820 

tctcaacacg actatgatag agatcaaata aagcgcgaat taaatagctt gcgacgcaac   2880 

gtgcacgatc tgtgcacgcg ttccggcacg agctttgatt gtaataagtt tttacgaagc   2940 

gatgacatga cccccgtagt gacaacgatc acgcccaaaa gaactgccga ctacaaaatt   3000 

accgagtatg tcggtgacgt taaaactatt aagccatcca atcgaccgtt agtcgaatca   3060 

ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca tcgtataacg tgtggagtcc   3120 

gctcattaga gcgtcatgtt tagacaagaa agctacatat ttaattgatc ccgatgattt   3180 

tattgataaa ttgaccctaa ctccatacac ggtattctac aatggcgggg ttttggtcaa   3240 

aatttccgga ctgcgattgt acatgctgtt aacggctccg cccactatta atgaaattaa   3300 

aaattccaat tttaaaaaac gcagcaagag aaacatttgt atgaaagaat gcgtagaagg   3360 

aaagaaaaat gtcgtcgaca tgctgaacaa caagattaat atgcctccgt gtataaaaaa   3420 

aatattgaac gatttgaaag aaaacaatgt accgcgcggc ggtatgtaca ggaagaggtt   3480 

tatactaaac tgttacattg caaacgtggt ttcgtgtgcc aagtgtgaaa accgatgttt   3540 

aatcaaggct ctgacgcatt tctacaacca cgactccaag tgtgtgggtg aagtcatgca   3600 

tcttttaatc aaatcccaag atgtgtataa accaccaaac tgccaaaaaa tgaaaactgt   3660 

cgacaagctc tgtccgtttg ctggcaactg caagggtctc aatcctattt gtaattattg   3720 

aataataaaa caattataaa tgctaaattt gttttttatt aacgatacaa accaaacgca   3780 

acaagaacat ttgtagtatt atctataatt gaaaacgcgt agttataatc gctgaggtaa   3840 

tatttaaaat cattttcaaa tgattcacag ttaatttgcg acaatataat tttattttca   3900 

cataaactag acgccttgtc gtcttcttct tcgtattcct tctctttttc atttttctcc   3960 

tcataaaaat taacatagtt attatcgtat ccatatatgt atctatcgta tagagtaaat   4020 

tttttgttgt cataaatata tatgtctttt ttaatggggt gtatagtacc gctgcgcata   4080 

gtttttctgt aatttacaac agtgctattt tctggtagtt cttcggagtg tgttgcttta   4140 

attattaaat ttatataatc aatgaatttg ggatcgtcgg ttttgtacaa tatgttgccg   4200 

gcatagtacg cagcttcttc tagttcaatt acaccatttt ttagcagcac cggattaaca   4260 

taactttcca aaatgttgta cgaaccgtta aacaaaaaca gttcacctcc cttttctata   4320 

ctattgtctg cgagcagttg tttgttgtta aaaataacag ccattgtaat gagacgcaca   4380 

aactaatatc acaaactgga aatgtctatc aatatatagt tgctgatatc atggagataa   4440 

ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt   4500 

aataaaaaaa cctataaata cggatccacc atg gtg agc aag ggc gag gag ctg    4554 
                                 Met Val Ser Lys Gly Glu Glu Leu 
                                   1               5 

ttc acc ggg gtg gtg ccc atc ctg gtc gag ctg gac ggc gac gta aac     4602 
Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
     10                  15                  20 

ggc cac aag ttc agc gtg tcc ggc gag ggc gag ggc gat gcc acc tac     4650 
Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr 
 25                  30                  35                  40 

ggc aag ctg acc ctg aag ttc atc tgc acc acc ggc aag ctg ccc gtg     4698 
Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
                 45                  50                  55 

ccc tgg ccc acc ctc gtg acc acc ctg acc tgg ggc gtg cag tgc ttc     4746 
Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Trp Gly Val Gln Cys Phe 
             60                  65                  70 

agc cgc tac ccc gac cac atg aag cag cac gac ttc ttc aag tcc gcc     4794 
Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
         75                  80                  85 

atg ccc gaa ggc tac gtc cag gag cgc acc atc ttc ttc aag gac gac     4842 
Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
     90                  95                 100 

ggc aac tac aag acc cgc gcc gag gtg aag ttc gag ggc gac acc ctg     4890 
Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
105                 110                 115                 120 

gtg aac cgc atc gag ctg aag ggc atc gac ttc aag gag gac ggc aac     4938 
Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
                125                 130                 135 

atc ctg ggg cac aag ctg gag tac aac tac aac agc cac aac gtc tat     4986 
Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
            140                 145                 150 

atc atg gcc gac aag cag aag aac ggc atc aag gtg aac ttc aag atc     5034 
Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile 
        155                 160                 165 

cgc cac aac atc gag gac ggc agc gtg cag ctc gcc gac cac tac cag     5082 
Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
    170                 175                 180 

cag aac acc ccc atc ggc gac ggc ccc gtg ctg ctg ccc gac aac cac     5130 
Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
185                 190                 195                 200 

tac ctg agc acc cag tcc gcc ctg agc aaa gac ccc aac gag aag cgc     5178 
Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 
                205                 210                 215 

gat cac atg gtc ctg ctg gag ttc gtg acc gcc gcc ggg atc act ctc     5226 
Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
            220                 225                 230 

ggc atg gac gag ctg tac aag  ggt accacaagtt tgtacaaaaa agctgaacga   5280 
Gly Met Asp Glu Leu Tyr Lys 
        235 

gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact   5340 

acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcacccctt   5400 

aggtagggtc accgtcgaca gcgacacact tgcatcggat gcagcccggt taacgtgccg   5460 

gcacggcctg ggtaaccagg tattttgtcc acataaccgt gcgcaaaatg ttgtggataa   5520 

gcaggacaca gcagcaatcc acagcaggca tacaaccgca caccgaggtt actccgttct   5580 

acaggttacg acgacatgtc aatacttgcc cttgacaggc attgatggaa tcgtagtctc   5640 

acgctgatag tctgatcgac aatacaagtg ggaccgtggt cccagaccga taatcagacc   5700 

gacaacacga gtgggatcgt ggtcccagac taataatcag accgacgata cgagtgggac   5760 

cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtt ccagactaat   5820 

aatcagaccg acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac   5880 

gagtgggacc atggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc   5940 

cagtctgatt atcagaccga cgatacgagt gggaccgtgg tcccagacta ataatcagac   6000 

cgacgatacg agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga   6060 

ccgtggtccc agtctgatta tcagaccgac gatacaagtg gaacagtggg cccagagaga   6120 

atattcaggc cagttatgct ttctggcctg taacaaagga cattaagtaa agacagataa   6180 

acgtagacta aaacgtggtc gcatcagggt gctggctttt caagttcctt aagaatggcc   6240 

tcaattttct ctatacactc agttggaaca cgagacctgt ccaggttaag caccatttta   6300 

tcgcccttat acaatactgt cgctccagga gcaaactgat gtcgtgagct taaactagtt   6360 

cttgatgcag atgacgtttt aagcacagaa gttaaaagag tgataacttc ttcagcttca   6420 

aatatcaccc cagctttttt ctgctcatga aggttagatg cctgctgctt aagtaattcc   6480 

tctttatctg taaaggcttt ttgaagtgca tcacctgacc gggcagatag ttcaccgggg   6540 

tgagaaaaaa gagcaacaac tgatttaggc aatttggcgg tgttgataca gcgggtaata   6600 

atcttacgtg aaatattttc cgcatcagcc agcgcagaaa tatttccagc aaattcattc   6660 

tgcaatcggc ttgcataacg ctgaccacgt tcataagcac ttgttgggcg ataatcgtta   6720 

cccaatctgg ataatgcagc catctgctca tcatccagct cgccaaccag aacacgataa   6780 

tcactttcgg taagtgcagc agctttacga cggcgactcc catcggcaat ttctatgaca   6840 

ccagatactc ttcgaccgaa cgccggtgtc tgttgaccag tcagtagaaa agaagggatg   6900 

agatcatcca gtgcgtcctc agtaagcagc tcctggtcac gttcattacc tgaccatacc   6960 

cgagaggtct tctcaacact atcaccccgg agcacttcaa gagtaaactt cacatcccga   7020 

ccacatacag gcaaagtaat ggcattaccg cgagccatta ctcctacgcg cgcaattaac   7080 

gaatccacca tcggggcagc tggtgtcgat aacgaagtat cttcaaccgg ttgagtattg   7140 

agcgtatgtt ttggaataac aggcgcacgc ttcattatct aatctcccag cgtggtttaa   7200 

tcagacgatc gaaaatttca ttgcagacag gttcccaaat agaaagagca tttctccagg   7260 

caccagttga agagcgttga tcaatggcct gttcaaaaac agttctcatc cggatctgac   7320 

ctttaccaac ttcatccgtt tcacgtacaa cattttttag aaccatgctt ccccaggcat   7380 

cccgaatttg ctcctccatc cacggggact gagagccatt actattgctg tatttggtaa   7440 

gcaaaatacg tacatcaggc tcgaaccctt taagatcaac gttcttgagc agatcacgaa   7500 

gcatatcgaa aaactgcagt gcggaggtgt agtcaaacaa ctcagcaggc gtgggaacaa   7560 

tcagcacatc agcagcacat acgacattaa tcgtgccgat acccaggtta ggcgcgctgt   7620 

caataactat gacatcatag tcatgagcaa cagtttcaat ggccagtcgg agcatcaggt   7680 

gtggatcggt gggcagttta ccttcatcaa atttgcccat taactcagtt tcaatacggt   7740 

gcagagccag acaggaagga ataatgtcaa gccccggcca gcaagtgggc tttattgcat   7800 

aagtgacatc gtccttttcc ccaagataga aaggcaggag agtgtcttct gcatgaatat   7860 

gaagatctgg tacccatccg tgatacattg aggctgttcc ctgggggtcg ttaccttcca   7920 

cgagcaaaac acgtagcccc ttcagagcca gatcctgagc aagatgaaca gaaactgagg   7980 

ttttgtaaac gccaccttta tgggcagcaa ccccgatcac cggtggaaat acgtcttcag   8040 

cacgtcgcaa tcgcgtacca aacacatcac gcatatgatt aatttgttca attgtataac   8100 

caacacgttg ctcaacccgt cctcgaattt ccatatccgg gtgcggtagt cgccctgctt   8160 

tctcggcatc tctgatagcc tgagaagaaa ccccaactaa atccgctgct tcacctattc   8220 

tccagcgccg ggttattttc ctcgcttccg ggctgtcatc attaaactgt gcaatggcga   8280 

tagccttcgt catttcatga ccagcgttta tgcactggtt aagtgtttcc atgagtttca   8340 

ttctgaacat cctttaatca ttgctttgcg tttttttatt aaatcttgca atttactgca   8400 

aagcaacaac aaaatcgcaa agtcatcaaa aaaccgcaaa gttgtttaaa ataagagcaa   8460 

cactacaaaa ggagataaga agagcacata cctcagtcac ttattatcac tagcgctcgc   8520 

cgcagccgtg taaccgagca tagcgagcga actggcgagg aagcaaagaa gaactgttct   8580 

gtcagatagc tcttacgctc agcgcaagaa gaaatatcca ccgtgggaaa aactccaggt   8640 

agaggtacac acgcggatag ccaattcaga gtaataaact gtgataatca accctcatca   8700 

atgatgacga actaaccccc gatatcaggt cacatgacga agggaaagag aaggaaatca   8760 

actgtgacaa actgccctca aatttggctt ccttaaaaat tacagttcaa aaagtatgag   8820 

aaaatccatg caggctgaag gaaacagcaa aactgtgaca aattaccctc agtaggtcag   8880 

aacaaatgtg acgaaccacc ctcaaatctg tgacagataa ccctcagact atcctgtcgt   8940 

catggaagtg atatcgcgga aggaaaatac gatatgagtc gtctggcggc ctttcttttt   9000 

ctcaatgtat gagaggcgca ttggagttct gctgttgatc tcattaacac agacctgcag   9060 

gaagcggcgg cggaagtcag gcatacgctg gtaactttga ggcagctggt aacgctctat   9120 

gatccagtcg attttcagag agacgatgcc tgagccatcc ggcttacgat actgacacag   9180 

ggattcgtat aaacgcatgg catacggatt ggtgatttct tttgtttcac taagccgaaa   9240 

ctgcgtaaac cggttctgta acccgataaa gaagggaatg agatatgggt tgatatgtac   9300 

actgtaaagc cctctggatg gactgtgcgc acgtttgata aaccaaggaa aagattcata   9360 

gcctttttca tcgccggcat cctcttcagg gcgataaaaa accacttcct tccccgcgaa   9420 

actcttcaat gcctgccgta tatccttact ggcttccgca gaggtcaatc cgaatatttc   9480 

agcatattta gcaacatgga tctcgcagat accgtcatgt tcctgtaggg tgccatcaga   9540 

ttttctgatc tggtcaacga acagatacag catacgtttt tgatcccggg agagactata   9600 

tgccgcctca gtgaggtcgt ttgactggac gattcgcggg ctatttttac gtttcttgtg   9660 

attgataacc gctgtttccg ccatgacaga tccatgtgaa gtgtgacaag tttttagatt   9720 

gtcacactaa ataaaaaaga gtcaataagc agggataact ttgtgaaaaa acagcttctt   9780 

ctgagggcaa tttgtcacag ggttaagggc aatttgtcac agacaggact gtcatttgag   9840 

ggtgatttgt cacactgaaa gggcaatttg tcacaacacc ttctctagaa ccagcatgga   9900 

taaaggccta caaggcgctc taaaaaagaa gatctaaaaa ctataaaaaa aataattata   9960 

aaaatatccc cgtggataag tggataaccc caagggaagt tttttcaggc atcgtgtgta  10020 

agcagaatat ataagtgctg ttccctggtg cttcctcgct cactcgaggg cttcgccctg  10080 

tcgctcaact gcggcgagca ctactggctg taaaaggaca gaccacatca tggttctgtg  10140 

ttcattaggt tgttctgtcc attgctgaca taatccgctc cacttcaacg taacaccgca  10200 

cgaagatttc tattgttcct gaaggcatat tcaaatcgtt ttcgttaccg cttgcaggca  10260 

tcatgacaga acactacttc ctataaacgc tacacaggct cctgagatta ataatgcgga  10320 

tctctacgat aatgggagat tttcccgact gtttcgttcg cttctcagtg gataacagcc  10380 

agcttctctg tttaacagac aaaaacagca tatccactca gttccacatt tccatataaa  10440 

ggccaaggca tttattctca ggataattgt ttcagcatcg caaccgcatc agactccggc  10500 

atcgcaaact gcacccggtg ccgggcagcc acatccagcg caaaaacctt cgtgtagact  10560 

tccgttgaac tgatggactt atgtcccatc aggctttgca gaactttcag cggtataccg  10620 

gcatacagca tgtgcatcgc ataggaatgg cggaacgtat gtggtgtgac cggaacagag  10680 

aacgtcacac cgtcagcagc agcggcggca accgcctccc caatccaggt cctgaccgtt  10740 

ctgtccgtca cttcccagat ccgcgctttc tctgtccttc ctgtgcgacg gttacgccgc  10800 

tccatgagct tatcgcgaat aaatacctgt gacggaagat cacttcgcag aataaataaa  10860 

tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt  10920 

tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt  10980 

attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac  11040 

tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca  11100 

gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa  11160 

gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct  11220 

gatgaatgct catccggagt tccgtatggc aatgaaagac ggtgagctgg tgatatggga  11280 

tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg  11340 

gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg  11400 

ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc  11460 

agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt  11520 

cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc  11580 

gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa  11640 

tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta  11700 

ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg  11760 

cagaaattcg atgataagct gtcaaacatg agaattggtc gacggcgcgc caaagcttgc  11820 

atgcctgcag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa tggatgccct  11880 

gcgtaagcgg ggcacatttc attacctctt tctccgcacc cgaccctcag gtcgaccata  11940 

gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat  12000 

ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg  12060 

tctcgagtta attaattgat ccgggttatt agtacattta ttaagcgcta gattctgtgc  12120 

gttgttgatt tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt  12180 

tagggtggta tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa  12240 

atattaaatc ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt  12300 

tttccgaacc gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca  12360 

aatcttgtag cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag  12420 

gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt  12480 

acaattgact cgacgtaaac acgttaaata aagcttggac atatttaaca tcgggcgtgt  12540 

tagctttatt aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg  12600 

aagacgattt tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga  12660 

tcaaatttgt agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt  12720 

tcaatctaac tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg  12780 

gtggtaacat ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat  12840 

ctaccatcgg tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg  12900 

gtgatgcaga cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa  12960 

ctattgtact ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt  13020 

ttcgtttct aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag   13080 

ttccgtcgg cattggtgga                                               13100