Abstract:
The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described.

Description:
CROSS-REFERENCE TO RELATED APPLICATIONS 
       [0001]    This application is a continuation of PCT/US2014/013189, filed on Jan. 27, 2014, which is related to U.S. Provisional Application No. 61/756,973, filed Jan. 25, 2013 and U.S. Provisional Application No. 61/826,637, filed May 23, 2013; each of which is herein incorporated by reference, in its entirety, for all purposes. 
     
    
     SEQUENCE LISTING 
       [0002]    The instant application contains a Sequence Listing which has been submitted via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Month XX, 20XX, is named XXXXXUS_sequencelisting.txt, and is X,XXX,XXX bytes in size. 
       BACKGROUND 
       [0003]    Many existing photoautotrophic organisms (i.e., plants, algae, and photosynthetic bacteria) are poorly suited for industrial bioprocessing and have therefore not demonstrated commercial viability. Recombinant photosynthetic microorganisms have been engineered to produce hydrocarbons and alcohols in amounts that exceed the levels produced naturally by the organism. 
       SUMMARY 
       [0004]    Described herein is an engineered microorganism, wherein said engineered microorganism comprises one or more recombinant genes encoding one or more enzymes having enzyme activities which catalyze the production of alkanes, wherein the enzyme activities comprise: an alkane deformylative monooxygenase activity, a thioesterase activity, a carboxylic acid reductase activity, and a phosphopanthetheinyl transferase activity; an alkane deformylative monooxygenase activity, a thioesterase activity, a long-chain fatty acid CoA-ligase activity, and a long-chain acyl-CoA reductase activity; and/or an alkane deformylative monooxygenase activity, a pyruvate decarboxylase activity and a 2-ketoacid decarboxylase activity. 
         [0005]    In some aspects, the enzymes comprise an alkane deformylative monooxygenase, a thioesterase, a carboxylic acid reductase, and a phosphopanthetheinyl transferase. In some aspects, the alkane deformylative monooxygenase has EC number 4.1.99.5, the thioesterase has EC number 3.1.2.14, the carboxylic acid reductase has EC number 1.2.99.6, and the phosphopanthetheinyl transferase has EC number 2.7.8.7. In some aspects, the alkane deformylative monooxygenase is encoded by adm, the thioesterase is encoded by fatB or fatB2, the carboxylic acid reductase is encoded by carB, and the phosphopanthetheinyl transferase is encoded by entD. 
         [0006]    In some aspects, the enzyme having alkane deformylative monooxygenase activity has EC number 4.1.99.5. In some aspects, the enzyme having thioesterase activity has EC number 3.1.2.14. In some aspects, the enzyme having carboxylic acid reductase activity has EC number 1.2.99.6. In some aspects, the enzyme having phosphopanthetheinyl transferase activity has EC number 2.7.8.7. 
         [0007]    In some aspects, the enzymes comprise an alkane deformylative monooxygenase, a thioesterase, a long-chain fatty acid CoA-ligase, and a long-chain acyl-CoA reductase. In some aspects, the alkane deformylative monooxygenase has EC number 4.1.99.5, the thioesterase has EC number 3.1.2.14, the long-chain fatty acid CoA-ligase has EC number 6.2.1.3, and the long-chain acyl-CoA reductase has EC number 1.2.1.50. In some aspects, the alkane deformylative monooxygenase is encoded by adm, the thioesterase is encoded by fatB or fatB2, the long-chain fatty acid CoA-ligase is encoded by fatD, and the long-chain acyl-CoA reductase is encoded by acrM. 
         [0008]    In some aspects, the enzyme having alkane deformylative monooxygenase activity has EC number 4.1.99.5. In some aspects, the enzyme having thioesterase activity has EC number 3.1.2.14. In some aspects, the enzyme having long-chain fatty acid CoA-ligase activity has EC number 6.2.1.3. In some aspects, the enzyme having long-chain acyl-CoA reductase activity has EC number 1.2.1.50. 
         [0009]    In some aspects, the one or more recombinant genes comprise a recombinant gene encoding a thioesterase that catalyzes the conversion of acyl-ACP to a fatty acid. In some aspects, the one or more recombinant genes comprises a recombinant gene encoding a phosphopanthetheinyl transferase that phosphopatetheinylates the ACP moiety of a protein encoded by a carboxylic acid reductase gene. In some aspects, the one or more recombinant genes comprise a recombinant gene encoding a carboxylic acid reductase that catalyzes the conversion of fatty acid to fatty aldehyde. In some aspects, the one or more recombinant genes comprise a recombinant gene encoding a alkane deformylative monooxygenase that catalyzes the conversion of fatty aldehyde to an alkane or alkene. In some aspects, the one or more recombinant genes comprise a recombinant gene encoding a fatty acid CoA-ligase that catalyzes the conversion of fatty acid to acyl-CoA. In some aspects, the one or more recombinant genes comprise a recombinant gene encoding an acyl-CoA reductase that catalyzes the conversion of acyl-CoA to fatty aldehyde. 
         [0010]    In some aspects, the enzymes comprise an alkane deformylative monooxygenase, a pyruvate decarboxylase and a 2-ketoacid decarboxylase. 
         [0011]    In some aspects, said microorganism is a bacterium. In some aspects, said microorganism is a gram-negative bacterium. In some aspects, said microorganism is  E. coli.    
         [0012]    In some aspects, said microorganism is a photosynthetic microorganism. In some aspects, said microorganism is a  cyanobacterium . In some aspects, said microorganism is a thermotolerant  cyanobacterium . In some aspects, said microorganism is a  Synechococcus  species. 
         [0013]    In some aspects, expression of an operon comprising the one or more recombinant genes is controlled by a recombinant promoter, and wherein the promoter is constitutive or inducible. In some aspects, said operon is integrated into the genome of said microorganism. In some aspects, said operon is extrachromosomal. 
         [0014]    In some aspects, said alkanes are less than or equal to 11 carbon atoms in length. In some aspects, said alkanes are 7 to 11 carbon atoms in length. In some aspects, said alkanes are 7, 8, 9, 10, or 11 carbon atoms in length. In some aspects, said alkanes are less than or equal to 18 carbon atoms in length. In some aspects, said alkanes are 7 to 18 carbon atoms in length. In some aspects, said alkanes are 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18 carbon atoms in length. 
         [0015]    In some aspects, said recombinant genes are at least 90% or at least 95% identical to a sequence shown in the Tables. 
         [0016]    Also described herein is a cell culture comprising a culture medium and a microorganism described herein. 
         [0017]    Also described herein is a method for producing hydrocarbons, comprising: culturing an engineered microorganism described herein in a culture medium, wherein said engineered microorganism produces increased amounts of alkanes relative to an otherwise identical microorganism, cultured under identical conditions, but lacking said recombinant genes. In some aspects, the method further includes allowing alkanes to accumulate in the culture medium or in the organism. In some aspects, the method further includes isolating at least a portion of the alkanes. In some aspects, the method further includes processing the isolated alkanes to produce a processed material. 
         [0018]    Also described herein is a method for producing hydrocarbons, comprising: (i) culturing an engineered microorganism described herein in a culture medium; and (ii) exposing said engineered microorganism to light and inorganic carbon, wherein said exposure results in the conversion of said inorganic carbon by said microorganism into alkanes, wherein said alkanes are produced in an amount greater than that produced by an otherwise identical microorganism, cultured under identical conditions, but lacking said recombinant genes. In some aspects, the method further includes allowing alkanes to accumulate in the culture medium or in the organism. In some aspects, the method further includes isolating at least a portion of the alkanes. In some aspects, the method further includes processing the isolated alkanes to produce a processed material. 
         [0019]    Also described herein is a composition comprising alkanes, wherein said alkanes are produced by a method described herein. In some aspects, the composition comprises at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 99% alkanes. 
         [0020]    The present invention provides, in certain embodiments a method of producing a short-chain alkane or alkene from an engineered organism, the method comprising: expressing a recombinant alkanal deformylative monooxygenase (“ADM”) in the engineered microorganism; culturing the engineered microorganism in a culture medium containing a carbon source under conditions effective to produce a short-chain alkane or alkene. 
         [0021]    In an embodiment, ADM catalyzes the conversion of an aldehyde into an alkane or alkene, wherein the aldehyde is selected from the group consisting of acetaldehyde, butanal, propanal, isobutanal, butanal, 3-methyl-1-butanal and 2-phenylethanal. In an embodiment, the alkane or alkene is selected from the group consisting of methane, propane, ethane, butane, propane, isobutane and toluene. In an embodiment, the method of producing a short-chain alkane or alkene from an engineered organism comprises expressing a recombinant pyruvate decarboxylase (“Pdc”) in the engineered microorganism. In certain embodiments, the Pdc is at least 90% identical SEQ ID NO: 46. In an embodiment, the method of producing a short-chain alkane or alkene from an engineered organism comprises expressing a 2-ketoacid decarboxylase in the engineered microorganism. In certain embodiments, the Pdc or the 2-ketoacid decarboxylase are expressed in an operon under the control of a single promoter. 
         [0022]    In an embodiment, the operon comprises ADM. In certain embodiments, the ADM is at least 90% identical to SEQ ID NO: 36. 
         [0023]    Also provided herein, are embodiments comprising an engineered microorganism, wherein the engineered microorganism comprises a recombinant gene encoding an alkanal deformylative monooxygenase (“ADM”), and wherein the engineered microorganism further comprises a recombinant gene encoding an enzyme selected from the group consisting of: pyruvate decarboxylase and 2-ketoacid decarboxylase. 
         [0024]    In one embodiment, the ADM catalyzes the conversion of an aldehyde into an alkane or alkene, wherein the aldehyde is selected from the group consisting of acetaldehyde, butanal, propanal, isobutanal, 2-methyl-1-butanal, butanal, 3-methyl-1-butanal and 2-phenylethanal. In certain embodiments, the alkane or alkene is selected from the group consisting of methane, propane, ethane, butane, propane, isobutane and toluene. 
         [0025]    In one embodiment, the engineered microorganism comprises a recombinant pyruvate decarboxylase (“Pdc”). In certain embodiments, the Pdc is at least 90% identical to SEQ ID NO: 46. In one embodiment, the engineered microorganism comprises a 2-ketoacid decarboxylase. In certain embodiments, the Pdc or the 2-ketoacid decarboxylase are expressed in an operons under the control of a single promoter. 
         [0026]    In one embodiment, the operon comprises ADM. In some embodiments, the engineered microorganism is an engineered  cyanobacterium . In certain embodiments, the ADM is at least 90% identical to SEQ ID NO: 36. 
         [0027]    Also provided herein, are embodiments comprising a cell culture comprising a recombinant microorganism and a culture medium containing a carbon source, wherein a polypeptide that catalyzes the conversion of an aldehyde to an alkane is overexpressed in the recombinant microorganism and an alkane or alkene is produced in the cell culture when the recombinant microorganism is cultured in the culture medium under conditions effective to express the polypeptide. In an embodiment, the polypeptide has alkanal deformylative monooxygenase activity. In an embodiment, the polypeptide comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 36. In some embodiments, the aldehyde is selected from the group consisting of acetaldehyde, butanal, propanal, isobutanal, butanal, 3-methyl-1-butanal, and 2-phenylethanal. 
         [0028]    In an embodiment, the alkane or alkene is selected from the group consisting of methane, propane, ethane, butane, propane, isobutane, and toluene. In an embodiment, the alkane is a short-chain alkane. In certain embodiments, the alkane comprises a C 2  to C 4  alkane. In some embodiments, the alkane comprises a C 2  to C 7  alkane. In an embodiment, the alkane or the alkene is secreted into the culture medium. 
         [0029]    In an embodiment, the recombinant microorganism further comprises a recombinant polypeptide comprising a pyruvate decarboxylase (“Pdc”) activity. In certain embodiments, the Pdc is at least 90% identical to SEQ ID NO: 46. In an embodiment, the recombinant microorganism further comprises a recombinant 2-ketoacid decarboxylase. In some embodiments, the Pdc or the 2-ketoacid decarboxylase are expressed in an operon under the control of a single promoter. In an embodiment, the operon comprises ADM. 
         [0030]    In an embodiment, the recombinant microorganism is selected from the group consisting of yeast, fungi, filamentous fungi, algae, and bacterium. In some embodiments, the bacterium is a  cyanobacterium.    
         [0031]    Also provided herein, are embodiments comprising a method for producing isobutane or a derivative of isobutane, comprising contacting ADM with an aldehyde in vitro. In an embodiment, the ADM is at least 90% identical to SEQ ID NO: 36. In certain embodiments, the ADM is  Nostoc punctiforme  ADM. In an embodiment, the aldehyde is 3-methylbutyraldehyde. 
         [0032]    These and other embodiments of the invention are further described in the Figures, Description, Examples and Claims, herein. 
     
    
     
       BRIEF DESCRIPTION OF THE FIGURES 
         [0033]      FIG. 1 . SDS-PAGE gel showing the overexpression of AcrM protein in  E. coli.    
           [0034]      FIG. 2 . TIC chromatograms of assays with (A) decanoyl-CoA, (B) lauroyl-CoA. Solid line: wild type BL21(DE3); dotted line: acrM-expressing BL21(DE3). 
           [0035]      FIG. 3 . GC/FID chromatogram showing the detection of C13 and C15 alkanes produced by  Synechococcus  sp. PCC 7002 strain expressing Adm, CarB, TesA and EntD proteins. Grey trace: control strain (does not express CarB protein); solid black trace: Standards of C13, C14, and C15 n-alkanes; dashed black trace:  Synechococcus  sp. PCC 7002 strain expressi-ng Adm, CarB, TesA, and EntD proteins. 
           [0036]      FIG. 4 . TIC chromatograms of samples from acid-fed (dashed lines) or control (solid lines)  Synechococcus  sp. PCC 7002 expressing Adm and CarB. A and D: octanoic acid feeding, B and E: decanoic acid feeding, C and F: dodecanoic acid feeding. 
           [0037]      FIG. 5 . GC/FID chromatogram showing the detection of nonane produced by  Synechococcus  sp. PCC 7002 strain expressing Adm, CarB, FatB2 and EntD proteins at 12 h and 72 h. Solid trace: control strain (wild type); dotted trace:  Synechococcus  sp. PCC 7002 strain expressing Adm, CarB, FatB2, and EntD proteins. 
           [0038]      FIG. 6 . Examples of pathways for production of alkanes. Note that the use of carB can be facilitated by the product of entD (phosphopanthetheinyl transferase), which phosphopatetheinylates the ACP moiety of the CarB protein. For example, one can use the  Bacillus  entD, whose enzyme product has a wide substrate spectrum that includes CarB. 
           [0039]      FIG. 7 . Detection of nonane (A) and undecane (B) produced by  Synechococcus  sp. PCC 7002 strain expressing Adm, thioesterase, CarB, and EntD proteins when fed with decanoic acid and dodecanoic acid. Circles: alkane detected in the cell pellet; triangles: alkane detected in the hexadecane overlay. 
           [0040]      FIG. 8 . GC/FID chromatograms showing the biosynthesis of nonane (A) and undecane (B) from CO 2 , by  Synechococcus  sp. PCC 7002 strain expressing Adm, thioesterase, CarB, and EntD proteins, secreted into the hexadecane overlay. Solid trace: samples from day 0; dotted trace: samples from day 5. 
           [0041]      FIG. 9 . Time course of the biosynthesis of undecane (triangle) and nonane (circle) from CO 2 , by  Synechococcus  sp. PCC 7002 strain expressing Adm, thioesterase, CarB, and EntD proteins, secreted into the hexadecane overlay. 
           [0042]      FIG. 10 . GC/FID chromatogram showing the detection of C13 and C15 alkanes produced by 7002 strain expressing Adm, CarB, TesA m  and EntD proteins. Solid line: control strain; dotted line: ALK-C13C15 (experimental strain). 
           [0043]      FIG. 11 . The growth curve of ALK-C13C15 over 10 days. 
           [0044]      FIG. 12 . The production curve of tridecane and pentadecane by ALK-C13C15 over 10 days. 
           [0045]      FIG. 13 . Depicts fractions from Ni-NTA purification of His 6 -tagged ADM enzyme. The collected fractions pooled for assay use are indicated. 
           [0046]      FIG. 14 . Time course of the biosynthesis of undecane (triangle) from CO 2  by JCC6036. 
           [0047]      FIG. 15 . Detection of nonane produced by 7002 strain expressing Adm, CarB, and EntD proteins when fed with decanoic acid. By expressing Nhistagged Adm on pAQ3, the initial activity was increased significantly compared to that on pAQ4. 
       
    
    
     DETAILED DESCRIPTION 
       [0048]    Unless otherwise defined herein, scientific and technical terms used in connection with the present invention shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include the plural and plural terms shall include the singular. Generally, nomenclatures used in connection with, and techniques of, biochemistry, enzymology, molecular and cellular biology, microbiology, genetics and protein and nucleic acid chemistry and hybridization described herein are those well known and commonly used in the art. 
         [0049]    The methods and techniques of the present invention are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification unless otherwise indicated. See, e.g., Sambrook et al.,  Molecular Cloning: A Laboratory Manual,  2d ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989); Ausubel et al.,  Current Protocols in Molecular Biology , Greene Publishing Associates (1992, and Supplements to 2002); Harlow and Lane,  Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1990); Taylor and Drickamer, Introduction to Glycobiology, Oxford Univ. Press (2003);  Worthington Enzyme Manual , Worthington Biochemical Corp., Freehold, N.J.;  Handbook of Biochemistry: Section A Proteins , Vol I, CRC Press (1976); Handbook of Biochemistry: Section A Proteins, Vol II, CRC Press (1976);  Essentials of Glycobiology , Cold Spring Harbor Laboratory Press (1999). 
         [0050]    All publications, patents and other references mentioned herein are hereby incorporated by reference in their entireties. 
         [0051]    The following terms, unless otherwise indicated, shall be understood to have the following meanings: 
         [0052]    The term “polynucleotide” or “nucleic acid molecule” refers to a polymeric form of nucleotides of at least 10 bases in length. The term includes DNA molecules (e.g., cDNA or genomic or synthetic DNA) and RNA molecules (e.g., mRNA or synthetic RNA), as well as analogs of DNA or RNA containing non-natural nucleotide analogs, non-native intemucleoside bonds, or both. The nucleic acid can be in any topological conformation. For instance, the nucleic acid can be single-stranded, double-stranded, triple-stranded, quadruplexed, partially double-stranded, branched, hairpinned, circular, or in a padlocked conformation. 
         [0053]    Unless otherwise indicated, and as an example for all sequences described herein under the general format “SEQ ID NO:”, “nucleic acid comprising SEQ ID NO:1” refers to a nucleic acid, at least a portion of which has either (i) the sequence of SEQ ID NO:1, or (ii) a sequence complementary to SEQ ID NO:1. The choice between the two is dictated by the context. For instance, if the nucleic acid is used as a probe, the choice between the two is dictated by the requirement that the probe be complementary to the desired target. 
         [0054]    An “isolated” RNA, DNA or a mixed polymer is one which is substantially separated from other cellular components that naturally accompany the native polynucleotide in its natural host cell, e.g., ribosomes, polymerases and genomic sequences with which it is naturally associated. 
         [0055]    As used herein, an “isolated” organic molecule (e.g., an alkane) is one which is substantially separated from the cellular components (membrane lipids, chromosomes, proteins) of the host cell from which it originated, or from the medium in which the host cell was cultured. The term does not require that the biomolecule has been separated from all other chemicals, although certain isolated biomolecules may be purified to near homogeneity. 
         [0056]    The term “recombinant” refers to a biomolecule, e.g., a gene or protein, that (1) has been removed from its naturally occurring environment, (2) is not associated with all or a portion of a polynucleotide in which the gene is found in nature, (3) is operatively linked to a polynucleotide which it is not linked to in nature, or (4) does not occur in nature. The term “recombinant” can be used in reference to cloned DNA isolates, chemically synthesized polynucleotide analogs, or polynucleotide analogs that are biologically synthesized by heterologous systems, as well as proteins and/or mRNAs encoded by such nucleic acids. 
         [0057]    As used herein, an endogenous nucleic acid sequence in the genome of an organism (or the encoded protein product of that sequence) is deemed “recombinant” herein if a heterologous sequence is placed adjacent to the endogenous nucleic acid sequence, such that the expression of this endogenous nucleic acid sequence is altered. In this context, a heterologous sequence is a sequence that is not naturally adjacent to the endogenous nucleic acid sequence, whether or not the heterologous sequence is itself endogenous (originating from the same host cell or progeny thereof) or exogenous (originating from a different host cell or progeny thereof). By way of example, a promoter sequence can be substituted (e.g., by homologous recombination) for the native promoter of a gene in the genome of a host cell, such that this gene has an altered expression pattern. This gene would now become “recombinant” because it is separated from at least some of the sequences that naturally flank it. 
         [0058]    A nucleic acid is also considered “recombinant” if it contains any modifications that do not naturally occur to the corresponding nucleic acid in a genome. For instance, an endogenous coding sequence is considered “recombinant” if it contains an insertion, deletion or a point mutation introduced artificially, e.g., by human intervention. A “recombinant nucleic acid” also includes a nucleic acid integrated into a host cell chromosome at a heterologous site and a nucleic acid construct present as an episome. 
         [0059]    As used herein, the phrase “degenerate variant” of a reference nucleic acid sequence encompasses nucleic acid sequences that can be translated, according to the standard genetic code, to provide an amino acid sequence identical to that translated from the reference nucleic acid sequence. The term “degenerate oligonucleotide” or “degenerate primer” is used to signify an oligonucleotide capable of hybridizing with target nucleic acid sequences that are not necessarily identical in sequence but that are homologous to one another within one or more particular segments. 
         [0060]    The term “percent sequence identity” or “identical” in the context of nucleic acid sequences refers to the residues in the two sequences which are the same when aligned for maximum correspondence. The length of sequence identity comparison may be over a stretch of at least about nine nucleotides, usually at least about 20 nucleotides, more usually at least about 24 nucleotides, typically at least about 28 nucleotides, more typically at least about 32 nucleotides, and preferably at least about 36 or more nucleotides. There are a number of different algorithms known in the art which can be used to measure nucleotide sequence identity. For instance, polynucleotide sequences can be compared using FASTA, Gap or Bestfit, which are programs in Wisconsin Package Version 10.0, Genetics Computer Group (GCG), Madison, Wis. FASTA provides alignments and percent sequence identity of the regions of the best overlap between the query and search sequences. Pearson, Methods Enzymol. 183:63-98 (1990) (hereby incorporated by reference in its entirety). For instance, percent sequence identity between nucleic acid sequences can be determined using FASTA with its default parameters (a word size of 6 and the NOPAM factor for the scoring matrix) or using Gap with its default parameters as provided in GCG Version 6.1, herein incorporated by reference. Alternatively, sequences can be compared using the computer program, BLAST (Altschul et al.,  J. Mol. Biol.  215:403-410 (1990); Gish and States,  Nature Genet.  3:266-272 (1993); Madden et al.,  Meth. Enzymol.  266:131-141 (1996); Altschul et al.,  Nucleic Acids Res.  25:3389-3402 (1997); Zhang and Madden,  Genome Res.  7:649-656 (1997)), especially blastp or tblastn (Altschul et al.,  Nucleic Acids Res.  25:3389-3402 (1997)). 
         [0061]    The term “substantial homology” or “substantial similarity,” when referring to a nucleic acid or fragment thereof, indicates that, when optimally aligned with appropriate nucleotide insertions or deletions with another nucleic acid (or its complementary strand), there is nucleotide sequence identity in at least about 76%, 80%, 85%, preferably at least about 90%, and more preferably at least about 95%, 96%, 97%, 98% or 99% of the nucleotide bases, as measured by any well-known algorithm of sequence identity, such as FASTA, BLAST or Gap, as discussed above. 
         [0062]    Alternatively, substantial homology or similarity exists when a nucleic acid or fragment thereof hybridizes to another nucleic acid, to a strand of another nucleic acid, or to the complementary strand thereof, under stringent hybridization conditions. “Stringent hybridization conditions” and “stringent wash conditions” in the context of nucleic acid hybridization experiments depend upon a number of different physical parameters. Nucleic acid hybridization will be affected by such conditions as salt concentration, temperature, solvents, the base composition of the hybridizing species, length of the complementary regions, and the number of nucleotide base mismatches between the hybridizing nucleic acids, as will be readily appreciated by those skilled in the art. One having ordinary skill in the art knows how to vary these parameters to achieve a particular stringency of hybridization. 
         [0063]    In general, “stringent hybridization” is performed at about 25° C. below the thermal melting point (T m ) for the specific DNA hybrid under a particular set of conditions. “Stringent washing” is performed at temperatures about 5° C. lower than the T m  for the specific DNA hybrid under a particular set of conditions. The T m  is the temperature at which 50% of the target sequence hybridizes to a perfectly matched probe. See Sambrook et al., Molecular Cloning: A Laboratory Manual, 2d ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989), page 9.51, hereby incorporated by reference. For purposes herein, “stringent conditions” are defined for solution phase hybridization as aqueous hybridization (i.e., free of formamide) in 6×SSC (where 20×SSC contains 3.0 M NaCl and 0.3 M sodium citrate), 1% SDS at 65° C. for 8-12 hours, followed by two washes in 0.2×SSC, 0.1% SDS at 65° C. for 20 minutes. It will be appreciated by the skilled worker that hybridization at 65° C. will occur at different rates depending on a number of factors including the length and percent identity of the sequences which are hybridizing. 
         [0064]    The nucleic acids (also referred to as polynucleotides) of this present invention may include both sense and antisense strands of RNA, cDNA, genomic DNA, and synthetic forms and mixed polymers of the above. They may be modified chemically or biochemically or may contain non-natural or derivatized nucleotide bases, as will be readily appreciated by those of skill in the art. Such modifications include, for example, labels, methylation, substitution of one or more of the naturally occurring nucleotides with an analog, intemucleotide modifications such as uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoramidates, carbamates, etc.), charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), pendent moieties (e.g., polypeptides), intercalators (e.g., acridine, psoralen, etc.), chelators, alkylators, and modified linkages (e.g., alpha anomeric nucleic acids, etc.) Also included are synthetic molecules that mimic polynucleotides in their ability to bind to a designated sequence via hydrogen bonding and other chemical interactions. Such molecules are known in the art and include, for example, those in which peptide linkages substitute for phosphate linkages in the backbone of the molecule. Other modifications can include, for example, analogs in which the ribose ring contains a bridging moiety or other structure such as the modifications found in “locked” nucleic acids. 
         [0065]    The term “mutated” when applied to nucleic acid sequences means that nucleotides in a nucleic acid sequence may be inserted, deleted or changed compared to a reference nucleic acid sequence. A single alteration may be made at a locus (a point mutation) or multiple nucleotides may be inserted, deleted or changed at a single locus. In addition, one or more alterations may be made at any number of loci within a nucleic acid sequence. A nucleic acid sequence may be mutated by any method known in the art including but not limited to mutagenesis techniques such as “error-prone PCR” (a process for performing PCR under conditions where the copying fidelity of the DNA polymerase is low, such that a high rate of point mutations is obtained along the entire length of the PCR product; see, e.g., Leung et al., Technique, 1:11-15 (1989) and Caldwell and Joyce, PCR Methods Applic. 2:28-33 (1992)); and “oligonucleotide-directed mutagenesis” (a process which enables the generation of site-specific mutations in any cloned DNA segment of interest; see, e.g., Reidhaar-Olson and Sauer, Science 241:53-57 (1988)). 
         [0066]    The term “attenuate” as used herein generally refers to a functional deletion, including a mutation, partial or complete deletion, insertion, or other variation made to a gene sequence or a sequence controlling the transcription of a gene sequence, which reduces or inhibits production of the gene product, or renders the gene product non-functional. In some instances a functional deletion is described as a knockout mutation. Attenuation also includes amino acid sequence changes by altering the nucleic acid sequence, placing the gene under the control of a less active promoter, down-regulation, expressing interfering RNA, ribozymes or antisense sequences that target the gene of interest, or through any other technique known in the art. In one example, the sensitivity of a particular enzyme to feedback inhibition or inhibition caused by a composition that is not a product or a reactant (non-pathway specific feedback) is lessened such that the enzyme activity is not impacted by the presence of a compound. In other instances, an enzyme that has been altered to be less active can be referred to as attenuated. 
         [0067]    Deletion: 
         [0068]    The removal of one or more nucleotides from a nucleic acid molecule or one or more amino acids from a protein, the regions on either side being joined together. 
         [0069]    Knock-Out: 
         [0070]    A gene whose level of expression or activity has been reduced to zero. In some examples, a gene is knocked-out via deletion of some or all of its coding sequence. In other examples, a gene is knocked-out via introduction of one or more nucleotides into its open reading frame, which results in translation of a non-sense or otherwise non-functional protein product. 
         [0071]    The term “vector” as used herein is intended to refer to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a “plasmid,” which generally refers to a circular double stranded DNA loop into which additional DNA segments may be ligated, but also includes linear double-stranded molecules such as those resulting from amplification by the polymerase chain reaction (PCR) or from treatment of a circular plasmid with a restriction enzyme. Other vectors include cosmids, bacterial artificial chromosomes (BAC) and yeast artificial chromosomes (YAC). Another type of vector is a viral vector, wherein additional DNA segments may be ligated into the viral genome (discussed in more detail below). Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., vectors having an origin of replication which functions in the host cell). Other vectors can be integrated into the genome of a host cell upon introduction into the host cell, and are thereby replicated along with the host genome. Moreover, certain preferred vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as “recombinant expression vectors” (or simply “expression vectors”). 
         [0072]    “Operatively linked” or “operably linked” expression control sequences refers to a linkage in which the expression control sequence is contiguous with the gene of interest to control the gene of interest, as well as expression control sequences that act in trans or at a distance to control the gene of interest. 
         [0073]    The term “expression control sequence” as used herein refers to polynucleotide sequences which are necessary to affect the expression of coding sequences to which they are operatively linked. Expression control sequences are sequences which control the transcription, post-transcriptional events and translation of nucleic acid sequences. Expression control sequences include appropriate transcription initiation, termination, promoter and enhancer sequences; efficient RNA processing signals such as splicing and polyadenylation signals; sequences that stabilize cytoplasmic mRNA; sequences that enhance translation efficiency (e.g., ribosome binding sites); sequences that enhance protein stability; and when desired, sequences that enhance protein secretion. The nature of such control sequences differs depending upon the host organism; in prokaryotes, such control sequences generally include promoter, ribosomal binding site, and transcription termination sequence. The term “control sequences” is intended to include, at a minimum, all components whose presence is essential for expression, and can also include additional components whose presence is advantageous, for example, leader sequences and fusion partner sequences. 
         [0074]    The term “recombinant host cell” (or simply “host cell”), as used herein, is intended to refer to a cell into which a recombinant vector has been introduced. It should be understood that such terms are intended to refer not only to the particular subject cell but to the progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term “host cell” as used herein. A recombinant host cell may be an isolated cell or cell line grown in culture or may be a cell which resides in a living tissue or organism. 
         [0075]    The term “peptide” as used herein refers to a short polypeptide, e.g., one that is typically less than about 50 amino acids long and more typically less than about 30 amino acids long. The term as used herein encompasses analogs and mimetics that mimic structural and thus biological function. 
         [0076]    The term “polypeptide” encompasses both naturally-occurring and non-naturally-occurring proteins, and fragments, mutants, derivatives and analogs thereof. A polypeptide may be monomeric or polymeric. Further, a polypeptide may comprise a number of different domains each of which has one or more distinct activities. 
         [0077]    The term “isolated protein” or “isolated polypeptide” is a protein or polypeptide that by virtue of its origin or source of derivation (1) is not associated with naturally associated components that accompany it in its native state, (2) exists in a purity not found in nature, where purity can be adjudged with respect to the presence of other cellular material (e.g., is free of other proteins from the same species) (3) is expressed by a cell from a different species, or (4) does not occur in nature (e.g., it is a fragment of a polypeptide found in nature or it includes amino acid analogs or derivatives not found in nature or linkages other than standard peptide bonds). Thus, a polypeptide that is chemically synthesized or synthesized in a cellular system different from the cell from which it naturally originates will be “isolated” from its naturally associated components. A polypeptide or protein may also be rendered substantially free of naturally associated components by isolation, using protein purification techniques well known in the art. As thus defined, “isolated” does not necessarily require that the protein, polypeptide, peptide or oligopeptide so described has been physically removed from its native environment. 
         [0078]    The term “polypeptide fragment” as used herein refers to a polypeptide that has a deletion, e.g., an amino-terminal and/or carboxy-terminal deletion compared to a full-length polypeptide. In a preferred embodiment, the polypeptide fragment is a contiguous sequence in which the amino acid sequence of the fragment is identical to the corresponding positions in the naturally-occurring sequence. Fragments typically are at least 5, 6, 7, 8, 9 or 10 amino acids long, preferably at least 12, 14, 16 or 18 amino acids long, more preferably at least 20 amino acids long, more preferably at least 25, 30, 35, 40 or 45, amino acids, even more preferably at least 50 or 60 amino acids long, and even more preferably at least 70 amino acids long. 
         [0079]    A “modified derivative” refers to polypeptides or fragments thereof that are substantially homologous in primary structural sequence but which include, e.g., in vivo or in vitro chemical and biochemical modifications or which incorporate amino acids that are not found in the native polypeptide. Such modifications include, for example, acetylation, carboxylation, phosphorylation, glycosylation, ubiquitination, labeling, e.g., with radionuclides, and various enzymatic modifications, as will be readily appreciated by those skilled in the art. A variety of methods for labeling polypeptides and of substituents or labels useful for such purposes are well known in the art, and include radioactive isotopes such as  125 I,  32 P,  35 S, and  3 H, ligands which bind to labeled antiligands (e.g., antibodies), fluorophores, chemiluminescent agents, enzymes, and antiligands which can serve as specific binding pair members for a labeled ligand. The choice of label depends on the sensitivity required, ease of conjugation with the primer, stability requirements, and available instrumentation. Methods for labeling polypeptides are well known in the art. See, e.g., Ausubel et al.,  Current Protocols in Molecular Biology , Greene Publishing Associates (1992, and Supplements to 2002) (hereby incorporated by reference). 
         [0080]    The term “fusion protein” refers to a polypeptide comprising a polypeptide or fragment coupled to heterologous amino acid sequences. Fusion proteins are useful because they can be constructed to contain two or more desired functional elements from two or more different proteins. A fusion protein comprises at least 10 contiguous amino acids from a polypeptide of interest, more preferably at least 20 or 30 amino acids, even more preferably at least 40, 50 or 60 amino acids, yet more preferably at least 75, 100 or 125 amino acids. Fusions that include the entirety of the proteins of the present invention have particular utility. The heterologous polypeptide included within the fusion protein of the present invention is at least 6 amino acids in length, often at least 8 amino acids in length, and usefully at least 15, 20, and 25 amino acids in length. Fusions that include larger polypeptides, such as an IgG Fc region, and even entire proteins, such as the green fluorescent protein (“GFP”) chromophore-containing proteins, have particular utility. Fusion proteins can be produced recombinantly by constructing a nucleic acid sequence which encodes the polypeptide or a fragment thereof in frame with a nucleic acid sequence encoding a different protein or peptide and then expressing the fusion protein. Alternatively, a fusion protein can be produced chemically by crosslinking the polypeptide or a fragment thereof to another protein. 
         [0081]    The term “non-peptide analog” refers to a compound with properties that are analogous to those of a reference polypeptide. A non-peptide compound may also be termed a “peptide mimetic” or a “peptidomimetic.” See, e.g., Jones,  Amino Acid and Peptide Synthesis , Oxford University Press (1992); Jung,  Combinatorial Peptide and Nonpeptide Libraries: A Handbook , John Wiley (1997); Bodanszky et al.,  Peptide Chemistry—A Practical Textbook , Springer Verlag (1993);  Synthetic Peptides: A Users Guide , (Grant, ed., W. H. Freeman and Co., 1992); Evans et al.,  J. Med. Chem.  30:1229 (1987); Fauchere, J.  Adv. Drug Res.  15:29 (1986); Veber and Freidinger,  Trends Neurosci.,  8:392-396 (1985); and references sited in each of the above, which are incorporated herein by reference. Such compounds are often developed with the aid of computerized molecular modeling. Peptide mimetics that are structurally similar to useful peptides of the present invention may be used to produce an equivalent effect and are therefore envisioned to be part of the present invention. 
         [0082]    A “polypeptide mutant” or “mutein” refers to a polypeptide whose sequence contains an insertion, duplication, deletion, rearrangement or substitution of one or more amino acids compared to the amino acid sequence of a native or wild-type protein. A mutein may have one or more amino acid point substitutions, in which a single amino acid at a position has been changed to another amino acid, one or more insertions and/or deletions, in which one or more amino acids are inserted or deleted, respectively, in the sequence of the naturally-occurring protein, and/or truncations of the amino acid sequence at either or both the amino or carboxy termini. A mutein may have the same but preferably has a different biological activity compared to the naturally-occurring protein. 
         [0083]    A mutein has at least 85% overall sequence homology to its wild-type counterpart. Even more preferred are muteins having at least 90% overall sequence homology to the wild-type protein. 
         [0084]    In an even more preferred embodiment, a mutein exhibits at least 95% sequence identity, even more preferably 98%, even more preferably 99% and even more preferably 99.9% overall sequence identity. 
         [0085]    Sequence homology may be measured by any common sequence analysis algorithm, such as Gap or Bestfit. 
         [0086]    Amino acid substitutions can include those which: (1) reduce susceptibility to proteolysis, (2) reduce susceptibility to oxidation, (3) alter binding affinity for forming protein complexes, (4) alter binding affinity or enzymatic activity, and (5) confer or modify other physicochemical or functional properties of such analogs. 
         [0087]    As used herein, the twenty conventional amino acids and their abbreviations follow conventional usage. See  Immunology—A Synthesis  (Golub and Gren eds., Sinauer Associates, Sunderland, Mass., 2 nd  ed. 1991), which is incorporated herein by reference. Stereoisomers (e.g., D-amino acids) of the twenty conventional amino acids, unnatural amino acids such as α-, α-disubstituted amino acids, N-alkyl amino acids, and other unconventional amino acids may also be suitable components for polypeptides of the present invention. Examples of unconventional amino acids include: 4-hydroxyproline, γ-carboxyglutamate, ε-N,N,N-trimethyllysine, ε-N-acetyllysine, O-phosphoserine, N-acetylserine, N-formylmethionine, 3-methylhistidine, 5-hydroxylysine, N-methylarginine, and other similar amino acids and imino acids (e.g., 4-hydroxyproline). In the polypeptide notation used herein, the left-hand end corresponds to the amino terminal end and the right-hand end corresponds to the carboxy-terminal end, in accordance with standard usage and convention. 
         [0088]    A protein has “homology” or is “homologous” to a second protein if the nucleic acid sequence that encodes the protein has a similar sequence to the nucleic acid sequence that encodes the second protein. Alternatively, a protein has homology to a second protein if the two proteins have “similar” amino acid sequences. (Thus, the term “homologous proteins” is defined to mean that the two proteins have similar amino acid sequences.) As used herein, homology between two regions of amino acid sequence (especially with respect to predicted structural similarities) is interpreted as implying similarity in function. 
         [0089]    When “homologous” is used in reference to proteins or peptides, it is recognized that residue positions that are not identical often differ by conservative amino acid substitutions. A “conservative amino acid substitution” is one in which an amino acid residue is substituted by another amino acid residue having a side chain (R group) with similar chemical properties (e.g., charge or hydrophobicity). In general, a conservative amino acid substitution will not substantially change the functional properties of a protein. In cases where two or more amino acid sequences differ from each other by conservative substitutions, the percent sequence identity or degree of homology may be adjusted upwards to correct for the conservative nature of the substitution. Means for making this adjustment are well known to those of skill in the art. See, e.g., Pearson, 1994 , Methods Mol. Biol.  24:307-31 and 25:365-89 (herein incorporated by reference). 
         [0090]    The following six groups each contain amino acids that are conservative substitutions for one another: 1) Serine (S), Threonine (T); 2) Aspartic Acid (D), Glutamic Acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Alanine (A), Valine (V), and 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W). 
         [0091]    Sequence homology for polypeptides, which is also referred to as percent sequence identity, is typically measured using sequence analysis software. See, e.g., the Sequence Analysis Software Package of the Genetics Computer Group (GCG), University of Wisconsin Biotechnology Center, 910 University Avenue, Madison, Wis. 53705. Protein analysis software matches similar sequences using a measure of homology assigned to various substitutions, deletions and other modifications, including conservative amino acid substitutions. For instance, GCG contains programs such as “Gap” and “Bestfit” which can be used with default parameters to determine sequence homology or sequence identity between closely related polypeptides, such as homologous polypeptides from different species of organisms or between a wild-type protein and a mutein thereof. See, e.g., GCG Version 6.1. 
         [0092]    A preferred algorithm when comparing a particular polypeptide sequence to a database containing a large number of sequences from different organisms is the computer program BLAST (Altschul et al.,  J. Mol. Biol.  215:403-410 (1990); Gish and States,  Nature Genet.  3:266-272 (1993); Madden et al.,  Meth. Enzymol.  266:131-141 (1996); Altschul et al.,  Nucleic Acids Res.  25:3389-3402 (1997); Zhang and Madden,  Genome Res.  7:649-656 (1997)), especially blastp or tblastn (Altschul et al.,  Nucleic Acids Res.  25:3389-3402 (1997)). 
         [0093]    Preferred parameters for BLASTp are: Expectation value: 10 (default); Filter: seg (default); Cost to open a gap: 11 (default); Cost to extend a gap: 1 (default); Max. alignments: 100 (default); Word size: 11 (default); No. of descriptions: 100 (default); Penalty Matrix: BLOWSUM62. 
         [0094]    The length of polypeptide sequences compared for homology will generally be at least about 16 amino acid residues, usually at least about 20 residues, more usually at least about 24 residues, typically at least about 28 residues, and preferably more than about 35 residues. When searching a database containing sequences from a large number of different organisms, it is preferable to compare amino acid sequences. Database searching using amino acid sequences can be measured by algorithms other than blastp known in the art. For instance, polypeptide sequences can be compared using FASTA, a program in GCG Version 6.1. FASTA provides alignments and percent sequence identity of the regions of the best overlap between the query and search sequences. Pearson,  Methods Enzymol.  183:63-98 (1990) (incorporated by reference herein). For example, percent sequence identity between amino acid sequences can be determined using FASTA with its default parameters (a word size of 2 and the PAM250 scoring matrix), as provided in GCG Version 6.1, herein incorporated by reference. 
         [0095]    “Specific binding” refers to the ability of two molecules to bind to each other in preference to binding to other molecules in the environment. Typically, “specific binding” discriminates over adventitious binding in a reaction by at least two-fold, more typically by at least 10-fold, often at least 100-fold. Typically, the affinity or avidity of a specific binding reaction, as quantified by a dissociation constant, is about 10 −7  M or stronger (e.g., about 10 −8  M, 10 −9  M or even stronger). 
         [0096]    The term “region” as used herein refers to a physically contiguous portion of the primary structure of a biomolecule. In the case of proteins, a region is defined by a contiguous portion of the amino acid sequence of that protein. 
         [0097]    The term “domain” as used herein refers to a structure of a biomolecule that contributes to a known or suspected function of the biomolecule. Domains may be co-extensive with regions or portions thereof; domains may also include distinct, non-contiguous regions of a biomolecule. Examples of protein domains include, but are not limited to, an Ig domain, an extracellular domain, a transmembrane domain, and a cytoplasmic domain. 
         [0098]    As used herein, the term “molecule” means any compound, including, but not limited to, a small molecule, peptide, protein, sugar, nucleotide, nucleic acid, lipid, etc., and such a compound can be natural or synthetic. 
         [0099]    “Carbon-based Products of Interest” include alcohols such as ethanol, propanol, isopropanol, butanol, fatty alcohols, fatty acid esters, wax esters; hydrocarbons and alkanes such as propane, octane, diesel, Jet Propellant 8 (JP8); polymers such as terephthalate, 1,3-propanediol, 1,4-butanediol, polyols, Polyhydroxyalkanoates (PHA), poly-beta-hydroxybutyrate (PHB), acrylate, adipic acid, ε-caprolactone, isoprene, caprolactam, rubber; commodity chemicals such as lactate, Docosahexaenoic acid (DHA), 3-hydroxypropionate, γ-valerolactone, lysine, serine, aspartate, aspartic acid, sorbitol, ascorbate, ascorbic acid, isopentenol, lanosterol, omega-3 DHA, lycopene, itaconate, 1,3-butadiene, ethylene, propylene, succinate, citrate, citric acid, glutamate, malate, 3-hydroxypropionic acid (HPA), lactic acid, THF, gamma butyrolactone, pyrrolidones, hydroxybutyrate, glutamic acid, levulinic acid, acrylic acid, malonic acid; specialty chemicals such as carotenoids, isoprenoids, itaconic acid; pharmaceuticals and pharmaceutical intermediates such as 7-aminodeacetoxycephalosporanic acid (7-ADCA)/cephalosporin, erythromycin, polyketides, statins, paclitaxel, docetaxel, terpenes, peptides, steroids, omega fatty acids and other such suitable products of interest. Such products are useful in the context of biofuels, industrial and specialty chemicals, as intermediates used to make additional products, such as nutritional supplements, neutraceuticals, polymers, paraffin replacements, personal care products and pharmaceuticals. 
         [0100]    Biofuel: A biofuel refers to any fuel that derives from a biological source. Biofuel can refer to one or more hydrocarbons, one or more alcohols (such as ethanol), one or more fatty esters, or a mixture thereof. 
         [0101]    Hydrocarbon: The term generally refers to a chemical compound that consists of the elements carbon (C), hydrogen (H) and optionally oxygen (O). There are essentially three types of hydrocarbons, e.g., aromatic hydrocarbons, saturated hydrocarbons and unsaturated hydrocarbons such as alkenes, alkynes, and dienes. The term also includes fuels, biofuels, plastics, waxes, solvents and oils. Hydrocarbons encompass biofuels, as well as plastics, waxes, solvents and oils. 
         [0102]    Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this present invention pertains. Exemplary methods and materials are described below, although methods and materials similar or equivalent to those described herein can also be used in the practice of the present invention and will be apparent to those of skill in the art. All publications and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. The materials, methods, and examples are illustrative only and not intended to be limiting. 
         [0103]    Throughout this specification and claims, the word “comprise” or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers. 
       Nucleic Acid Sequences 
       [0104]    The present invention provides isolated nucleic acid molecules for genes encoding enzymes, and variants thereof. Exemplary full-length nucleic acid sequences for genes encoding enzymes and the corresponding amino acid sequences are presented in Tables 1 and 2. 
         [0105]    In one embodiment, the present invention provides an isolated nucleic acid molecule having a nucleic acid sequence comprising or consisting of a gene coding for an alkane deformylative monooxygenase, a thioesterase, a carboxylic acid reductase, a phosphopanthetheinyl transferase, a long-chain fatty acid CoA-ligase, and/or a long-chain acyl-CoA reductase and homologs, variants and derivatives thereof expressed in a host cell of interest. The present invention also provides a nucleic acid molecule comprising or consisting of a sequence which is a codon-optimized version of the alkane deformylative monooxygenase, a thioesterase, a carboxylic acid reductase, a phosphopanthetheinyl transferase, a long-chain fatty acid CoA-ligase, and/or a long-chain acyl-CoA reductase genes described herein. In a further embodiment, the present invention provides a nucleic acid molecule and homologs, variants and derivatives of the molecule comprising or consisting of a sequence which is a variant of the alkane deformylative monooxygenase, a thioesterase, a carboxylic acid reductase, a phosphopanthetheinyl transferase, a long-chain fatty acid CoA-ligase, and/or a long-chain acyl-CoA reductase gene having at least 80% identity to the wild-type gene. The nucleic acid sequence can be preferably greater than 80%, 85%, 90%, 95%, 98%, 99%, 99.9% or even higher identity to the wild-type gene. 
         [0106]    In another embodiment, the nucleic acid molecule of the present invention encodes a polypeptide having an amino acid sequence disclosed in Tables 1 and 2. Preferably, the nucleic acid molecule of the present invention encodes a polypeptide sequence of at least 50%, 60, 70%, 80%, 85%, 90% or 95% identity to the amino acid sequences shown in Tables 1 and 2 and the identity can even more preferably be 96%, 97%, 98%, 99%, 99.9% or even higher. 
         [0107]    The present invention also provides nucleic acid molecules that hybridize under stringent conditions to the above-described nucleic acid molecules. As defined above, and as is well known in the art, stringent hybridizations are performed at about 25° C. below the thermal melting point (T m ) for the specific DNA hybrid under a particular set of conditions, where the T m  is the temperature at which 50% of the target sequence hybridizes to a perfectly matched probe. Stringent washing is performed at temperatures about 5° C. lower than the T m  for the specific DNA hybrid under a particular set of conditions. 
         [0108]    Nucleic acid molecules comprising a fragment of any one of the above-described nucleic acid sequences are also provided. These fragments preferably contain at least 20 contiguous nucleotides. More preferably the fragments of the nucleic acid sequences contain at least 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100 or even more contiguous nucleotides. 
         [0109]    The nucleic acid sequence fragments of the present invention display utility in a variety of systems and methods. For example, the fragments may be used as probes in various hybridization techniques. Depending on the method, the target nucleic acid sequences may be either DNA or RNA. The target nucleic acid sequences may be fractionated (e.g., by gel electrophoresis) prior to the hybridization, or the hybridization may be performed on samples in situ. One of skill in the art will appreciate that nucleic acid probes of known sequence find utility in determining chromosomal structure (e.g., by Southern blotting) and in measuring gene expression (e.g., by Northern blotting). In such experiments, the sequence fragments are preferably detectably labeled, so that their specific hydridization to target sequences can be detected and optionally quantified. One of skill in the art will appreciate that the nucleic acid fragments of the present invention may be used in a wide variety of blotting techniques not specifically described herein. 
         [0110]    It should also be appreciated that the nucleic acid sequence fragments disclosed herein also find utility as probes when immobilized on microarrays. Methods for creating microarrays by deposition and fixation of nucleic acids onto support substrates are well known in the art. Reviewed in  DNA Microarrays: A Practical Approach  (Practical Approach Series), Schena (ed.), Oxford University Press (1999) (ISBN: 0199637768); Nature Genet. 21(1)(suppl):1-60 (1999);  Microarray Biochip: Tools and Technology , Schena (ed.), Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN: 1881299376), the disclosures of which are incorporated herein by reference in their entireties. Analysis of, for example, gene expression using microarrays comprising nucleic acid sequence fragments, such as the nucleic acid sequence fragments disclosed herein, is a well-established utility for sequence fragments in the field of cell and molecular biology. Other uses for sequence fragments immobilized on microarrays are described in Gerhold et al.,  Trends Biochem. Sci.  24:168-173 (1999) and Zweiger,  Trends Biotechnol.  17:429-436 (1999);  DNA Microarrays: A Practical Approach  (Practical Approach Series), Schena (ed.), Oxford University Press (1999) (ISBN: 0199637768); Nature Genet. 21(1)(suppl):1-60 (1999);  Microarray Biochip: Tools and Technology , Schena (ed.), Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN: 1881299376), the disclosure of each of which is incorporated herein by reference in its entirety. 
         [0111]    As is well known in the art, enzyme activities can be measured in various ways. For example, the pyrophosphorolysis of OMP may be followed spectroscopically (Grubmeyer et al., (1993)  J. Biol. Chem.  268:20299-20304). Alternatively, the activity of the enzyme can be followed using chromatographic techniques, such as by high performance liquid chromatography (Chung and Sloan, (1986)  J. Chromatogr.  371:71-81). As another alternative the activity can be indirectly measured by determining the levels of product made from the enzyme activity. These levels can be measured with techniques including aqueous chloroform/methanol extraction as known and described in the art (Cf. M. Kates (1986)  Techniques of Lipidology; Isolation, analysis and identification of Lipids . Elsevier Science Publishers, New York (ISBN: 0444807322)). More modern techniques include using gas chromatography linked to mass spectrometry (Niessen, W. M. A. (2001).  Current practice of gas chromatography—mass spectrometry . New York, N.Y: Marcel Dekker. (ISBN: 0824704738)). Additional modern techniques for identification of recombinant protein activity and products including liquid chromatography-mass spectrometry (LCMS), high performance liquid chromatography (HPLC), capillary electrophoresis, Matrix-Assisted Laser Desorption Ionization time of flight-mass spectrometry (MALDI-TOF MS), nuclear magnetic resonance (NMR), near-infrared (NIR) spectroscopy, viscometry (Knothe, G (1997)  Am. Chem. Soc. Symp. Series,  666: 172-208), titration for determining free fatty acids (Komers (1997)  Fett/Lipid,  99(2): 52-54), enzymatic methods (Bailer (1991)  Fresenius J. Anal. Chem.  340(3): 186), physical property-based methods, wet chemical methods, etc. can be used to analyze the levels and the identity of the product produced by the organisms of the present invention. Other methods and techniques may also be suitable for the measurement of enzyme activity, as would be known by one of skill in the art. 
       Vectors 
       [0112]    Also provided are vectors, including expression vectors, which comprise the above nucleic acid molecules of the present invention, as described further herein. In a first embodiment, the vectors include the isolated nucleic acid molecules described above. In an alternative embodiment, the vectors of the present invention include the above-described nucleic acid molecules operably linked to one or more expression control sequences. The vectors of the instant invention may thus be used to express a polypeptide contributing to alkane producing activity by a host cell. 
         [0113]    Vectors useful for expression of nucleic acids in prokaryotes are well known in the art. 
       Isolated Polypeptides 
       [0114]    According to another aspect of the present invention, isolated polypeptides (including muteins, allelic variants, fragments, derivatives, and analogs) encoded by the nucleic acid molecules of the present invention are provided. In one embodiment, the isolated polypeptide comprises the polypeptide sequence corresponding to a polypeptide sequence shown in Table 1 or 2. In an alternative embodiment of the present invention, the isolated polypeptide comprises a polypeptide sequence at least 85% identical to a polypeptide sequence shown in Table 1 or 2. Preferably the isolated polypeptide of the present invention has at least 50%, 60, 70%, 80%, 85%, 90%, 95%, 98%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9% or even higher identity to a polypeptide sequence shown in Table 1 or 2. 
         [0115]    According to other embodiments of the present invention, isolated polypeptides comprising a fragment of the above-described polypeptide sequences are provided. These fragments preferably include at least 20 contiguous amino acids, more preferably at least 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100 or even more contiguous amino acids. 
         [0116]    The polypeptides of the present invention also include fusions between the above-described polypeptide sequences and heterologous polypeptides. The heterologous sequences can, for example, include sequences designed to facilitate purification, e.g. histidine tags, and/or visualization of recombinantly-expressed proteins. Other non-limiting examples of protein fusions include those that permit display of the encoded protein on the surface of a phage or a cell, fusions to intrinsically fluorescent proteins, such as green fluorescent protein (GFP), and fusions to the IgG Fc region. 
       Host Cell Transformants 
       [0117]    In another aspect of the present invention, host cells transformed with the nucleic acid molecules or vectors of the present invention, and descendants thereof, are provided. In some embodiments of the present invention, these cells carry the nucleic acid sequences of the present invention on vectors, which may but need not be freely replicating vectors. In other embodiments of the present invention, the nucleic acids have been integrated into the genome of the host cells. 
         [0118]    In an alternative embodiment, the host cells of the present invention can be mutated by recombination with a disruption, deletion or mutation of the isolated nucleic acid of the present invention so that the activity of one or more enzyme(s) in the host cell is reduced or eliminated compared to a host cell lacking the mutation. 
       Selected or Engineered Microorganisms for the Production of Carbon-Based Products of Interest 
       [0119]    Microorganism: Includes prokaryotic and eukaryotic microbial species from the Domains Archaea, Bacteria and Eucarya, the latter including yeast and filamentous fungi, protozoa, algae, or higher Protista. The terms “microbial cells” and “microbes” are used interchangeably with the term microorganism. 
         [0120]    A variety of host organisms can be transformed to produce a product of interest. Photoautotrophic organisms include eukaryotic plants and algae, as well as prokaryotic cyanobacteria, green-sulfur bacteria, green non-sulfur bacteria, purple sulfur bacteria, and purple non-sulfur bacteria. 
         [0121]    Extremophiles are also contemplated as suitable organisms. Such organisms withstand various environmental parameters such as temperature, radiation, pressure, gravity, vacuum, desiccation, salinity, pH, oxygen tension, and chemicals. They include hyperthermophiles, which grow at or above 80° C. such as  Pyrolobus fumarii ; thermophiles, which grow between 60-80° C. such as  Synechococcus lividis ; mesophiles, which grow between 15-60° C. and psychrophiles, which grow at or below 15° C. such as  Psychrobacter  and some insects. Radiation tolerant organisms include  Deinococcus radiodurans . Pressure-tolerant organisms include piezophiles, which tolerate pressure of 130 MPa. Weight-tolerant organisms include barophiles. Hypergravity (e.g., &gt;1 g) hypogravity (e.g., &lt;1 g) tolerant organisms are also contemplated. Vacuum tolerant organisms include tardigrades, insects, microbes and seeds. Dessicant tolerant and anhydrobiotic organisms include xerophiles such as  Artemia salina ; nematodes, microbes, fungi and lichens. Salt-tolerant organisms include halophiles (e.g., 2-5 M NaCl)  Halobacteriacea  and  Dunaliella salina . pH-tolerant organisms include alkaliphiles such as  Natronobacterium, Bacillus firmus  OF4 , Spirulina  spp. (e.g., pH&gt;9) and acidophiles such as  Cyanidium caldarium, Ferroplasma  sp. (e.g., low pH). Anaerobes, which cannot tolerate O 2  such as  Methanococcus jannaschii ; microaerophils, which tolerate some O 2  such as  Clostridium  and aerobes, which require O 2  are also contemplated. Gas-tolerant organisms, which tolerate pure CO 2  include  Cyanidium caldarium  and metal tolerant organisms include metalotolerants such as  Ferroplasma acidarmanus  (e.g., Cu, As, Cd, Zn),  Ralstonia  sp. CH34 (e.g., Zn, Co, Cd, Hg, Pb). Gross, Michael.  Life on the Edge: Amazing Creatures Thriving in Extreme Environments . New York: Plenum (1998) and Seckbach, J. “Search for Life in the Universe with Terrestrial Microbes Which Thrive Under Extreme Conditions.” In Cristiano Batalli Cosmovici, Stuart Bowyer, and Dan Wertheimer, eds.,  Astronomical and Biochemical Origins and the Search for Life in the Universe , p. 511. Milan: Editrice Compositori (1997). 
         [0122]    Plants include but are not limited to the following genera:  Arabidopsis, Beta, Glycine, Jatropha, Miscanthus, Panicum, Phalaris, Populus, Saccharum, Salix, Simmondsia  and  Zea.    
         [0123]    Algae and cyanobacteria include but are not limited to the following genera:  Acanthoceras, Acanthococcus, Acaryochloris, Achnanthes, Achnanthidium, Actinastrum, Actinochloris, Actinocyclus, Actinotaenium, Amphichrysis, Amphidinium, Amphikrikos, Amphipleura, Amphiprora, Amphithrix, Amphora, Anabaena, Anabaenopsis, Aneumastus, Ankistrodesmus, Ankyra, Anomoeoneis, Apatococcus, Aphanizomenon, Aphanocapsa, Aphanochaete, Aphanothece, Apiocystis, Apistonema, Arthrodesmus, Artherospira, Ascochloris, Asterionella, Asterococcus, Audouinella, Aulacoseira, Bacillaria, Balbiania, Bambusina, Bangia, Basichlamys, Batrachospermum, Binuclearia, Bitrichia, Blidingia, Botrdiopsis, Botrydium, Botryococcus, Botryosphaerella, Brachiomonas, Brachysira, Brachytrichia, Brebissonia, Bulbochaete, Bumilleria, Bumilleriopsis, Caloneis, Calothrix, Campylodiscus, Capsosiphon, Carteria, Catena, Cavinula, Centritractus, Centronella, Ceratium, Chaetoceros, Chaetochloris, Chaetomorpha, Chaetonella, Chaetonema, Chaetopeltis, Chaetophora, Chaetosphaeridium, Chamaesiphon, Chara, Characiochloris, Characiopsis, Characium, Charales, Chilomonas, Chlainomonas, Chlamydoblepharis, Chlamydocapsa, Chlamydomonas, Chlamydomonopsis, Chlamydomyxa, Chlamydonephris, Chlorangiella, Chlorangiopsis, Chlorella, Chlorobotrys, Chlorobrachis, Chlorochytrium, Chlorococcum, Chlorogloea, Chlorogloeopsis, Chlorogonium, Chlorolobion, Chloromonas, Chlorophysema, Chlorophyta, Chlorosaccus, Chlorosarcina, Choricystis, Chromophyton, Chromulina, Chroococcidiopsis, Chroococcus, Chroodactylon, Chroomonas, Chroothece, Chrysamoeba, Chrysapsis, Chrysidiastrum, Chrysocapsa, Chrysocapsella, Chrysochaete, Chrysochromulina, Chrysococcus, Chrysocrinus, Chrysolepidomonas, Chrysolykos, Chrysonebula, Chiysophyta, Chrysopyxis, Chrysosaccus, Chrysophaerella, Chrysostephanosphaera, Clodophora, Clastidium, Closteriopsis, Closterium, Coccomyxa, Cocconeis, Coelastrella, Coelastrum, Coelosphaerium, Coenochloris, Coenococcus, Coenocystis, Colacium, Coleochaete, Collodictyon, Compsogonopsis, Compsopogon, Conjugatophyta, Conochaete, Coronastrum, Cosmarium, Cosmioneis, Cosmocladium, Crateriportula, Craticula, Crinalium, Crucigenia, Crucigeniella, Cryptoaulax, Cryptomonas, Cryptophyta, Ctenophora, Cyanodictyon, Cyanonephron, Cyanophora, Cyanophyta, Cyanothece, Cyanothomonas, Cyclonexis, Cyclostephanos, Cyclotella, Cylindrocapsa, Cylindrocystis, Cylindrospermum, Cylindrotheca, Cymatopleura, Cymbella, Cymbellonitzschia, Cystodinium Dactylococcopsis, Debarya, Denticula, Dermatochrysis, Dermocarpa, Dermocarpella, Desmatractum, Desmidium, Desmococcus, Desmonema, Desmosiphon, Diacanthos, Diacronema, Diadesmis, Diatoma, Diatomella, Dicellula, Dichothrix, Dichotomococcus, Dicranochaete, Dictyochloris, Dictyococcus, Dictyosphaerium, Didymocystis, Didymogenes, Didymosphenia, Dilabifilum, Dimorphococcus, Dinobryon, Dinococcus, Diplochloris, Diploneis, Diplostauron, Distrionella, Docidium, Draparnaldia, Dunaliella, Dysmorphococcus, Ecballocystis, Elakatothrix, Ellerbeckia, Encyonema, Enteromorpha, Entocladia, Entomoneis, Entophysalis, Epichrysis, Epipyxis, Epithemia, Eremosphaera, Euastropsis, Euastrum, Eucapsis, Eucocconeis, Eudorina, Euglena, Euglenophyta, Eunotia, Eustigmatophyta, Eutreptia, Fallacia, Fischerella, Fragilaria, Fragilariforma, Franceia, Frustulia, Curcilla, Geminella, Genicularia, Glaucocystis, Glaucophyta, Glenodiniopsis, Glenodinium, Gloeocapsa, Gloeochaete, Gloeochrysis, Gloeococcus, Gloeocystis, Gloeodendron, Gloeomonas, Gloeoplax, Gloeothece, Gloeotila, Gloeotrichia, Gloiodictyon, Golenkinia, Golenkiniopsis, Gomontia, Gomphocymbella, Gomphonema, Gomphosphaeria, Gonatozygon, Gongrosia, Gongrosira, Goniochloris, Gonium, Gonyostomum, Granulochloris, Granulocystopsis, Groenbladia, Gymnodinium, Gymnozyga, Gyrosigma, Haematococcus, Hafniomonas, Hallassia, Hammatoidea, Hannaea, Hantzschia, Hapalosiphon, Haplotaenium, Haptophyta, Haslea, Hemidinium, Hemitoma, Heribaudiella, Heteromastix, Heterothrix, Hibberdia, Hildenbrandia, Hillea, Holopedium, Homoeothrix, Hormanthonema, Hormotila, Hyalobrachion, Hyalocardium, Hyalodiscus, Hyalogonium, Hyalotheca, Hydrianum, Hydrococcus, Hydrocoleum, Hydrocoryne, Hydrodictyon, Hydrosera, Hydrurus, Hyella, Hymenomonas, Isthmochloron, Johannesbaptistia, Juranyiella, Karayevia, Kathablepharis, Katodinium, Kephyrion, Keratococcus, Kirchneriella, Klebsormidium, Kolbesia, Koliella, Komarekia, Korshikoviella, Kraskella, Lagerheimia, Lagynion, Lamprothamnium, Lemanea, Lepocinclis, Leptosira, Lobococcus, Lobocystis, Lobomonas, Luticola, Lyngbya, Malleochloris, Mallomonas, Mantoniella, Marssoniella, Martyana, Mastigocoleus, Gastogloia, Melosira, Merismopedia, Mesostigma, Mesotaenium, Micractinium, Micrasterias, Microchaete, Microcoleus, Microcystis, Microglena, Micromonas, Microspora, Microthamnion, Mischococcus, Monochrysis, Monodus, Monomastix, Monoraphidium, Monostroma, Mougeotia, Mougeotiopsis, Myochloris, Myromecia, Myxosarcina, Naegeliella, Nannochloris, Nautococcus, Navicula, Neglectella, Neidium, Nephroclamys, Nephrocytium, Nephrodiella, Nephroselmis, Netrium, Nitella, Nitellopsis, Nitzschia, Nodularia, Nostoc, Ochromonas, Oedogonium, Oligochaetophora, Onychonema, Oocardium, Oocystis, Opephora, Ophiocytium, Orthoseira, Oscillatoria, Oxyneis, Pachycladella, Palmella, Palmodictyon, Pnadorina, Pannus, Paralia, Pascherina, Paulschulzia, Pediastrum, Pedinella, Pedinomonas, Pedinopera, Pelagodictyon, Penium, Peranema, Peridiniopsis, Peridinium, Peronia, Petroneis, Phacotus, Phacus, Phaeaster, Phaeodermatium, Phaeophyta, Phaeosphaera, Phaeothamnion, Phormidium, Phycopeltis, Phyllariochloris, Phyllocardium, Phyllomitas, Pinnularia, Pitophora, Placoneis, Planctonema, Planktosphaeria, Planothidium, Plectonema, Pleodorina, Pleurastrum, Pleurocapsa, Pleurocladia, Pleurodiscus, Pleurosigma, Pleurosira, Pleurotaenium, Pocillomonas, Podohedra, Polyblepharides, Polychaetophora, Polyedriella, Polyedriopsis, Polygoniochloris, Polyepidomonas, Polytaenia, Polytoma, Polytomella, Porphyridium, Posteriochromonas, Prasinochloris, Prasinocladus, Prasinophyta, Prasiola, Prochlorphyta, Prochlorothrix, Protoderma, Protosiphon, Provasoliella, Prymnesium, Psammodictyon, Psammothidium, Pseudanabaena, Pseudenoclonium, Psuedocarteria, Pseudochate, Pseudocharacium, Pseudococcomyxa, Pseudodictyosphaerium, Pseudokephyrion, Pseudoncobyrsa, Pseudoquadrigula, Pseudosphaerocystis, Pseudostaurastrum, Pseudostaurosira, Pseudotetrastrum, Pteromonas, Punctastruata, Pyramichlamys, Pyramimonas, Pyrrophyta, Quadrichloris, Quadricoccus, Quadrigula, Radiococcus, Radiofilum, Raphidiopsis, Raphidocelis, Raphidonema, Raphidophyta, Peimeria, Rhabdoderma, Rhabdomonas, Rhizoclonium, Rhodomonas, Rhodophyta, Rhoicosphenia, Rhopalodia, Rivularia, Rosenvingiella, Rossithidium, Roya, Scenedesmus, Scherffelia, Schizochlamydella, Schizochlamys, Schizomeris, Schizothrix, Schroederia, Scolioneis, Scotiella, Scotiellopsis, Scourfieldia, Scytonema, Selenastrum, Selenochloris, Sellaphora, Semiorbis, Siderocelis, Diderocystopsis, Dimonsenia, Siphononema, Sirocladium, Sirogonium, Skeletonema, Sorastrum, Spermatozopsis, Sphaerellocystis, Sphaerellopsis, Sphaerodinium, Sphaeroplea, Sphaerozosma, Spiniferomonas, Spirogyra, Spirotaenia, Spirulina, Spondylomorum, Spondylosium, Sporotetras, Spumella, Staurastrum, Stauerodesmus, Stauroneis, Staurosira, Staurosirella, Stenopterobia, Stephanocostis, Stephanodiscus, Stephanoporos, Stephanosphaera, Stichococcus, Stichogloea, Stigeoclonium, Stigonema, Stipitococcus, Stokesiella, Strombomonas, Stylochrysalis, Stylodinium, Styloyxis, Stylosphaeridium, Surirella, Sykidion, Symploca, Synechococcus, Synechocystis, Synedra, Synochromonas, Synura, Tabellaria, Tabularia, Teilingia, Temnogametum, Tetmemorus, Tetrachlorella, Tetracyclus, Tetradesmus, Tetraedriella, Tetraedron, Tetraselmis, Tetraspora, Tetrastrum, Thalassiosira, Thamniochaete, Thorakochloris, Thorea, Tolypella, Tolypothrix, Trachelomonas, Trachydiscus, Trebouxia, Trentepholia, Treubaria, Tribonema, Trichodesmium, Trichodiscus, Trochiscia, Tryblionella, Ulothrix, Uroglena, Uronema, Urosolenia, Urospora, Uva, Vacuolaria, Vaucheria, Volvox, Volvulina, Westella, Woloszynskia, Xanthidium, Xanthophyta, Xenococcus, Zygnema, Zygnemopsis , and Zygonium. Cyanobacteria include members of the genus  Chamaesiphon, Chroococcus, Cyanobacterium, Cyanobium, Cyanothece, Dactylococcopsis, Gloeobacter, Gloeocapsa, Gloeothece, Microcystis, Prochlorococcus, Prochloron, Synechococcus, Synechocystis, Cyanocystis, Dermocarpella, Stanieria, Xenococcus, Chroococcidiopsis, Myxosarcina, Arthrospira, Borzia, Crinalium, Geitlerinemia, Leptolyngbya, Limnothrix, Lyngbya, Microcoleus, Oscillatoria, Planktothrix, Prochiorothrix, Pseudanabaena, Spirulina, Starria, Symploca, Trichodesmium, Tychonema, Anabaena, Anabaenopsis, Aphanizomenon, Cyanospira, Cylindrospermopsis, Cylindrospermum, Nodularia, Nostoc, Scylonema, Calothrix, Rivularia, Tolypothrix, Chlorogloeopsis, Fischerella, Geitieria, Iyengariella, Nostochopsis, Stigonema  and  Thermosynechococcus.    
         [0124]    Green non-sulfur bacteria include but are not limited to the following genera:  Chloroflexus, Chloronema, Oscillochloris, Heliothrix, Herpetosiphon, Roseiflexus , and  Thermomicrobium.    
         [0125]    Green sulfur bacteria include but are not limited to the following genera: 
         [0126]      Chlorobium, Clathrochloris , and  Prosthecochloris.    
         [0127]    Purple sulfur bacteria include but are not limited to the following genera:  Allochromatium, Chromatium, Halochromatium, Isochromatium, Marichromatium, Rhodovulum, Thermochromatium, Thiocapsa, Thiorhodococcus , and  Thiocystis,    
         [0128]    Purple non-sulfur bacteria include but are not limited to the following genera:  Phaeospirillum, Rhodobaca, Rhodobacter, Rhodomicrobium, Rhodopila, Rhodopseudomonas, Rhodothalassium, Rhodospirillum, Rodovibrio , and  Roseospira.    
         [0129]    Aerobic chemolithotrophic bacteria include but are not limited to nitrifying bacteria such as  Nitrobacteraceae  sp.,  Nitrobacter  sp.,  Nitrospina  sp.,  Nitrococcus  sp.,  Nitrospira  sp.,  Nitrosomonas  sp.,  Nitrosococcus  sp.,  Nitrosospira  sp.,  Nitrosolobus  sp.,  Nitrosovibrio  sp.; colorless sulfur bacteria such as,  Thiovulum  sp.,  Thiobacillus  sp.,  Thiomicrospira  sp.,  Thiosphaera  sp.,  Thermothrix  sp.; obligately chemolithotrophic hydrogen bacteria such as  Hydrogenobacter  sp., iron and manganese-oxidizing and/or depositing bacteria such as  Siderococcus  sp., and magnetotactic bacteria such as  Aquaspirillum  sp. 
         [0130]    Archaeobacteria include but are not limited to methanogenic archaeobacteria such as  Methanobacterium  sp.,  Methanobrevibacter  sp.,  Methanothermus  sp.,  Methanococcus  sp.,  Methanomicrobium  sp.,  Methanospirillum  sp.,  Methanogenium  sp.,  Methanosarcina  sp.,  Methanolobus  sp.,  Methanothrix  sp.,  Methanococcoides  sp.,  Methanoplanus  sp.; extremely thermophilic S-Metabolizers such as  Thermoproteus  sp.,  Pyrodictium  sp.,  Sulfolobus  sp.,  Acidianus  sp. and other microorganisms such as,  Bacillus subtilis, Saccharomyces cerevisiae, Streptomyces  sp.,  Ralstonia  sp.,  Rhodococcus  sp.,  Corynebacteria  sp.,  Brevibacteria  sp.,  Mycobacteria  sp., and oleaginous yeast. 
         [0131]    Preferred organisms for the manufacture of alkanes according to the methods disclosed herein include:  Arabidopsis thaliana, Panicum virgatum, Miscanthus giganteus , and  Zea mays  (plants);  Botryococcus braunii, Chlamydomonas reinhardtii  and  Dunaliela salina  (algae);  Synechococcus  sp PCC 7002 , Synechococcus  sp. PCC 7942,  Synechocystis  sp. PCC 6803 , Thermosynechococcus elongatus  BP-1 (cyanobacteria);  Chlorobium tepidum  (green sulfur bacteria),  Chloroflexus auranticus  (green non-sulfur bacteria);  Chromatium tepidum  and  Chromatium vinosum  (purple sulfur bacteria);  Rhodospirillum rubrum, Rhodobacter capsulatus , and  Rhodopseudomonas palusris  (purple non-sulfur bacteria). 
         [0132]    Yet other suitable organisms include synthetic cells or cells produced by synthetic genomes as described in Venter et al. US Pat. Pub. No. 2007/0264688, and cell-like systems or synthetic cells as described in Glass et al. US Pat. Pub. No. 2007/0269862. 
         [0133]    Still, other suitable organisms include microorganisms that can be engineered to fix carbon dioxide bacteria such as  Escherichia coli, Acetobacter aceti, Bacillus subtilis , yeast and fungi such as  Clostridium ljungdahlii, Clostridium thermocellum, Penicillium chrysogenum, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pseudomonas fluorescens , or  Zymomonas mobilis.    
         [0134]    A suitable organism for selecting or engineering is capable of autotrophic fixation of CO 2  to products. This would cover photosynthesis and methanogenesis. Acetogenesis, encompassing the three types of CO 2  fixation; Calvin cycle, acetyl-CoA pathway and reductive TCA pathway is also covered. The capability to use carbon dioxide as the sole source of cell carbon (autotrophy) is found in almost all major groups of prokaryotes. The CO 2  fixation pathways differ between groups, and there is no clear distribution pattern of the four presently-known autotrophic pathways. See, e.g., Fuchs, G. 1989 . Alternative pathways of autotrophic CO   2    fixation , p. 365-382. In H. G. Schlegel, and B. Bowien (ed.),  Autotrophic bacteria . Springer-Verlag, Berlin, Germany. The reductive pentose phosphate cycle (Calvin-Bassham-Benson cycle) represents the CO 2  fixation pathway in almost all aerobic autotrophic bacteria, for example, the cyanobacteria. 
         [0135]    Alkane production via engineered cyanobacteria, e.g., a  Synechococcus  or  Thermosynechococcus  species, is preferred. Other preferred organisms include  Synechocystis, Klebsiella oxytoca, Escherichia coli  or  Saccharomyces cerevisiae . Other prokaryotic, archaea and eukaryotic host cells are also encompassed within the scope of the present invention. 
         [0136]    In some aspects, alkane production via a photosynthetic organism can be carried out using the compositions, materials, and methods described in: PCT/US2009/035937 (filed Mar. 3, 2009); and PCT/US2009/055949 (filed Sep. 3, 2009); each of which is herein incorporated by reference in its entirety, for all purposes. 
       Carbon-Based Products of Interest: Hydrocarbons &amp; Alcohols 
       [0137]    In various embodiments of the invention, desired hydrocarbons and/or alcohols of certain chain length or a mixture thereof can be produced. In certain aspects, the host cell produces at least one of the following carbon-based products of interest: alkanes such as heptane, nonane, tridecane, pentadecane, and/or undecane. In other aspects, the carbon chain length ranges from C 2  to C 20 , e.g., C 2 , C 3 , C 4 , C 5 , C 6 , C 7 , C 8 , C 9 , C 10 , C 11 , C 12 , C 13 , C 14 , C 15 , C 16 , C 17 , C 18 , C 19 , or C 20 . Accordingly, the invention provides production of various chain lengths of alkanes suitable for use as fuels &amp; chemicals. 
         [0138]    In preferred aspects, the methods provide culturing host cells for direct product secretion for easy recovery without the need to extract biomass. These carbon-based products of interest are secreted directly into the medium. Since the invention enables production of various defined chain length of hydrocarbons and alcohols, the secreted products are easily recovered or separated. The products of the invention, therefore, can be used directly or used with minimal processing. 
       Fuel Compositions 
       [0139]    In various embodiments, compositions produced by the methods of the invention are used as fuels. Such fuels comply with ASTM standards, for instance, standard specifications for diesel fuel oils D 975-09b, and Jet A, Jet A-1 and Jet B as specified in ASTM Specification D. 1655-68. Fuel compositions may require blending of several products to produce a uniform product. The blending process is relatively straightforward, but the determination of the amount of each component to include in a blend is much more difficult. Fuel compositions may, therefore, include aromatic and/or branched hydrocarbons, for instance, 75% saturated and 25% aromatic, wherein some of the saturated hydrocarbons are branched and some are cyclic. Preferably, the methods of the invention produce an array of hydrocarbons, such as C 2 -C 17  or C 10 -C 15  to alter cloud point. Furthermore, the compositions may comprise fuel additives, which are used to enhance the performance of a fuel or engine. For example, fuel additives can be used to alter the freezing/gelling point, cloud point, lubricity, viscosity, oxidative stability, ignition quality, octane level, and flash point. Fuels compositions may also comprise, among others, antioxidants, static dissipater, corrosion inhibitor, icing inhibitor, biocide, metal deactivator and thermal stability improver. 
         [0140]    In addition to many environmental advantages of the invention such as CO 2  conversion and renewable source, other advantages of the fuel compositions disclosed herein include low sulfur content, low emissions, being free or substantially free of alcohol and having high cetane number. 
       Example 1 
     Crude Extract of  E. coli  Cells Overexpressing acrM Convert Lauroyl-CoA to Dodecanal and Decanoyl-CoA to Decanal 
       [0141]      Acinetobacter  sp. M-1 acyl coenzyme A reductase, acrM, was codon-optimized for  E. coli  expression and synthesized by DNA2.0 (Menlo Park, Calif.; SEQ ID NO. 1) with a NdeI site on the 5′ end and an EcoRI site on the 3′end. The obtained gene was subcloned into a pET28a vector (Novagen) by digestion with NdeI and EcoRI and subsequent ligation. The resulting plasmid, pET28a-acrM (SEQ ID NO. 2), containing an N-terminal His 6 -tagged acrM, was transformed into a BL21(DE3)  E. coli  strain purchased from New England Biolabs, which was subsequently grown with shaking in Luria-Bertani medium supplemented with 100 μg/mL of kanomycin in a volume of 1 L to OD 600 =0.8 before induction with 0.25 mM Isopropyl β-D-1-thiogalactopyranoside for 5 hours in a 2-L shaker flask at 37° C. An SDS-PAGE gel demonstrating the overexpression of AcrM protein in pET28a-acrM containing BL21(DE3)  E. coli  cells is shown in  FIG. 1 . 
         [0142]    The  E. coli  cells containing overexpressed AcrM were collected by centrifugation, resuspended in HEPES buffer (100 mM HEPES, 10% glycerol, pH 7.5) at a 1:3 (w/v) ratio and lysed by sonication. 200 μL of buffer solution containing 100 μL total lysate, 1 mM acyl-CoA, 3 mM NADH (Sigma-Aldrich), 100 mM HEPES, 10% glycerol at pH 7.5 was incubated at 37° C. for 30 min, extracted with 100 μL ethyl acetate and analyzed by GC/MS equipped with a HP-5 ms column (Agilent, Santa Clara, Calif.). Total ion chromatography (TIC) indicated the detection of aldehydes produced from corresponding acyl-CoA substrates by the AcrM-containing cell extract in the presence of supplemented NADH, as shown in  FIG. 2 , indicating that AcrM is able to convert lauroyl-CoA to dodecanal and decanoyl-CoA to decanal. 
       Example 2 
     Feeding Fatty Acid to  Synechococcus  Sp. PCC 7002 Strain Expressing Adm-carB-entD Results in Detection of Corresponding Aldehyde and Alkane 
       [0143]    The carboxylic acid reductase (carB) gene (SEQ ID NO. 3) was PCR-amplified from  Mycobacterium smegmatis  and verified by sequencing with multiple primers by Genewiz (South Plainfield, N.J.).  Cyanothece  adm,  E. coli  leaderless tesA and  E. coli  entD genes were codon-optimized for  E. coli  overexpression and synthesized by DNA 2.0 (Menlo Park, Calif.; SEQ ID NO. 4 and 5) with an individual ribosome binding site in front of each gene. All four genes were subcloned into a pUC19 vector containing an ammonia-repressible P(nir07) promoter (U.S. Pat. No. 7,955,820), upstream/downstream homology regions, and a spectinomycin marker. The resulting plasmid, pAQ3::P(nir07)-adm-carB-tesA-entD-SpecR (SEQ ID NO. 6), was transformed into wild-type  Synechococcus  sp. PCC 7002 and segregated in the presence of spectinomycin. 
         [0144]    The expression and activity of the Adm, CarB, TesA, and EntD proteins were demonstrated by detection of tridecane and pentadecane in the transformed  Synechococcus  sp. PCC 7002 strain by GC/FID ( FIG. 3 ). 
         [0145]    The  Synechococcus  sp. PCC 7002 cultures were grown to OD 730 ˜5 before 1 mM fatty acid (100 mM stock in ethanol) was added and were then shaken at 150 rpm, 37° C. for ˜3 hours in the absence (lauric acid feeding) or presence (octanoic acid and decanoic acid feeding) of a pentadecane overlay (6 mL culture with 1 mL overlay). The pentadecane overlay from the octanoic acid-fed culture ( FIGS. 4A and 4D ), or decanoic acid culture ( FIGS. 4B and 4E ) was analyzed by GC/MS equipped with an HP-5 ms column. For the lauric acid feeding assay, 1 mL culture was extracted with 400 μL hexane by vortexing for 1 min before being analyzed by GC/MS ( FIG. 4C ,  4 F). Note that the pAQ3::P(nir07)-adm-carB-tesA-entD-SpecR expressing  Synechococcus  sp. PCC 7002 strain can produce a detectable level of undecane even without feeding dodecanoic acid. Adm and carB together is able to produce undecane in vivo. 
       Example 3 
       Synechococcus  sp. PCC 7002 Strain Expressing Adm-carB-fatB2-entD Results in Increased Detection of Nonane in Pentadecane Overlay 
       [0146]    The  E. coli  leaderless tesA of pAQ3::P(nir07)-adm-carB-tesA-entD-SpecR, was replaced by  Cuphea hookeriana  leaderless fatB2 (a medium-chain acyl-ACP thioesterase), which was codon-optimized for  E. coli  overexpression and synthesized by DNA 2.0 (Menlo Park, Calif.; SEQ ID NO. 7), with an individual ribosome binding site in front of the gene, a 5′ Kpn I restriction site and a 3′ Hind III restriction site. The resulting plasmid, pAQ3::P(nir07)-adm-carB-fatB2-entD-SpecR (SEQ ID NO. 8), was transformed into wild-type  Synechococcus  sp. PCC 7002 and segregated in the presence of spectinomycin. 
         [0147]    The wild type  Synechococcus  sp. PCC 7002 and pAQ3::P(nir07)-adm-carB-fatB2-entD-SpecR expressing  Synechococcus  sp. PCC 7002 cultures (35 mL) were grown in JB3.0 media (Table A below) to OD 730 ˜3 (in the presence of 2 mM urea) before a 10 mL pentadecane overlay was added. 
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE A 
               
             
             
               
                   
               
               
                 JB3.0 Media 
               
             
          
           
               
                   
                   
                 Amount 
                   
                 Calculated 
               
               
                   
                 Ingredient 
                 per liter 
                 Units 
                 Amount 
               
               
                   
                   
               
             
          
           
               
                   
                 NaCl 
                 18 
                 g 
                 36 
               
               
                   
                 Citric Acid 
                 1 
                 g 
                 2 
               
               
                   
                 KCl 
                 0.6 
                 g 
                 1.2 
               
               
                   
                 NaNO 3   
                 5.1 
                 g 
                 10.2 
               
               
                   
                 500 g/l 
                 10 
                 mL 
                 20 
               
               
                   
                 MgSO 4 •7H 2 O 
               
               
                   
                 50 g/l KH 2 PO 4   
                 4.6 
                 mL 
                 9.2 
               
               
                   
                 17.76 g/l CaCl 2   
                 15 
                 mL 
                 30 
               
               
                   
                 3 g/l NaEDTA tetra   
                 10 
                 mL 
                 20 
               
               
                   
                 3.52 g/l Ferric 
                 4.83 
                 mL 
                 9.66 
               
               
                   
                 Citrate (in 0.1N HCl) 
               
               
                   
                 0.88M Tris (pH 8.2) 
                 9.375 
                 mL 
                 18.75 
               
               
                   
                 P1 Metals 
                 1 
                 mL 
                 2 
               
               
                   
                 Solution 
               
               
                   
                 MilliQ H 2 O 
                 950 
                 mL 
                 1900 
               
               
                   
                 4 mg/l Vitamin B 12   
                 1 
                 mL 
                 2 
               
               
                   
                   
               
             
          
         
       
     
         [0148]    The cultures were shaken at 150 rpm, 37° C. for 3 more days continuously. 100 μL pentadecane overlay samples from each flask were taken 12 hours ( FIG. 5A ) or 72 hours ( FIG. 5B ) after pentadecane addition, respectively, and analyzed directly by GC/FID equipped with a 20 meter hp-5 ms column. An increase of nonane production was detected in the pAQ3::P(nir07)-adm-carB-fatB2-entD-SpecR expressing  Synechococcus  sp. PCC 7002 cultures but not in the wild type control ones. A relative increase in octane and heptanes production was also detected in the pAQ3::P(nir07)-adm-carB-fatB2-entD-SpecR expressing  Synechococcus  sp. PCC 7002 cultures. Adm, CarB and FatB2 together produced nonane in vivo. Shorter alkanes can also be produced via Adm-CarB pathway if shorter fatty acids are provided in vivo. 
       Example 4 
     Alkane Production 
       [0149]    One or more recombinant genes encoding one or more enzymes having enzyme activities which catalyze the production of alkanes are identified and selected. The enzyme activities include: an alkane deformylative monooxygenase activity, a thioesterase activity, a carboxylic acid reductase activity, and a phosphopanthetheinyl transferase activity, a long-chain fatty acid CoA-ligase activity, and/or a long-chain acyl-CoA reductase activity. Such genes and enzymes can be those described in Tables 1 and 2. 
         [0150]    The selected genes are cloned into an expression vector. For example, adm-carB-entD-fatB or adm-acrM-fadD-fatB (or combinations of homologs thereof) are cloned into one or more vectors. See  FIG. 6 . The genes can be under inducible control (such as the urea-repressible nir07 promoter or the cumate-inducible cum02 promoter). The genes may or may not be expressed operonically; and one or more of the genes can be placed under constitutive control such that when the other gene(s) are induced, the genes under constitutive control are already expressed. For example, one might express adm, carB, and entD constitutively while placing fatty-acid-generating fatB under inducible control; thus when fatty acids are made by fatB after induction, the remainder of the pathway is already present. 
         [0151]    One or more vectors are selected and transformed into a microorganism (e.g., cyanobacteria). The cells are grown to a suitable optical density. In some instances cells are grown to a suitable optical density in an uninduced state, and then an induction signal is applied to commence alkane production. 
         [0152]    Alkanes are produced by the transformed cells. The alkanes generally have 7, 8, 9, 10, 11 or more carbon atoms. In some instances, alkanes are detected. In some instances, alkanes are quantified. In some instances, alkanes are collected. 
         [0153]    In some aspects, a thioesterase such as fatB can be used. To test downstream of fatB, fatty acids of various chain lengths are fed along with inorganic carbon (e.g., CO 2 ) to cells, and alkane production is monitored. After fatB addition, cells are provided with inorganic carbon (e.g., CO 2 ) and alkane production is monitored. 
       Example 5 
     Feeding Decanoic Acid and Dodecanoic Acid to Adm, Thioesterase and carB/entD Expressing  Synechococcus  Sp. PCC 7002 Strain Results in Detection of Corresponding Nonane and Undecane with Secretion 
       [0154]    Carboxylic acid reductase (carB) (SEQ ID NO. 18) was PCR amplified from  Mycobacterium smegmatis  and verified by sequencing with multiple primers by Genewiz.  Nostoc punctiforme  adm,  Umbellularia californicia  fatB m  (where subscript “m” indicates mature protein, i.e., without leader sequence), and  E. coli  entD genes were codon-optimized for  E. coli  overexpression and synthesized by DNA 2.0 (Menlo Park, Calif.; SEQ ID NOs. 19, 20, and 21). The adm gene was subcloned into a pUC19 vector with a P(cpcB) promoter (U.S. Pat. No. 7,794,969), upstream/downstream homology regions, and an erythromycin marker. The resulting plasmid (pAQ4::P(cpcB)-adm Npu -ermC (SEQ ID NO. 22)) was transformed into wild-type  Synechococcus  sp. PCC 7002 strain and segregated in the presence of erythromycin (which resulted in strain ADM). The fatB m , carB, and entD genes were subcloned into a pUC19 vector containing a P(nir07) promoter, upstream/downstream homology regions, and a spectinomycin marker. The resulting plasmid (pAQ3::P(nir07)-fatB m -carB-entD-SpecR (SEQ ID NO. 23)) was transformed into the strain ADM and segregated in the presence of the antibiotic spectinomycin. 
         [0155]    The culture of the above final strain was grown in JB3.0 media till OD 730 ˜6 at 37° C., 150 rpm, and with 2% CO 2 , in the presence of 15 mM urea. The cells were spun down, resuspended in fresh media without urea, and grown overnight to allow the expression of proteins regulated under the P(nir07) promoter. An overlay of 1.5 mL hexadecane was then added onto the 6 mL culture before 0.1 mM decanoic acid or dodecanoic acid (200 mM stock, dissolved in 100% ethanol) was fed into the culture every 2 hours. At 2 and 4 hours, 0.15 mL of the overlay (triangle) and 0.6 mL of the aqueous culture sample (circle) were collected and analyzed by GC/FID equipped with an hp-5 ms column. When fed with decanoic acid, nonane was produced in vivo with an initial rate of &gt;2.2 mg/L/h, &gt;90% of which was secreted into the overlay ( FIG. 7A ). When fed with dodecanoic acid, undecane was produced in vivo with an initial rate of 1.2 mg/L/h, ˜50% of which was secreted after 4 hours ( FIG. 7B ). This indicates that the undecane product is spontaneously secreted to the overlay outside the cells overtime. 
       Example 6 
     Biosynthesis of Nonane and Undecane by  Synechococcus  Sp. PCC 7002 Strain Expressing Adm, Thioesterase and carB/entD with Secretion 
       [0156]    Carboxylic acid reductase (carB) (SEQ ID NO. 24) was PCR amplified from  Mycobacterium smegmatis  and verified by sequencing with multiple primers by Genewiz.  Nostoc punctiforme  adm,  Umbellularia californicia  fatB m  (where subscript “m” indicates mature protein, i.e. without leader sequence),  Cuphea hookeriana  fatB2 m , and  E. coli  entD genes were codon-optimized for  E. coli  overexpression and synthesized by DNA 2.0 (Menlo Park, Calif.; SEQ ID NOs. 25, 26, 27, and 28). The adm gene was subcloned into a pUC19 vector with P(cpcB) promoter, upstream/downstream homology regions, and an erythromycin marker. The resulting plasmid (pAQ4::P(cpcB)-adm Npu -ermC (SEQ ID NO. 29)) was transformed into wild-type  Synechococcus  sp. PCC 7002 strain and segregated in the presence of erythromycin (which resulted in strain ADM). The fatB m , carB, and entD genes were subcloned into a pUC19 vector containing a P(nir07) promoter, upstream/downstream homology regions, and a spectinomycin marker. The resulting plasmid (pAQ3::P(nir07)-fatB m -carB-entD-SpecR (SEQ ID NO. 30)) was transformed into the strain ADM and segregated in the presence of the antibiotic spectinomycin, resulting in strain ALK-C11. The fatB2 m , carB, and entD genes were subcloned into a pUC19 vector containing a P(nir07) promoter, upstream/downstream homology regions, and a spectinomycin marker. The resulting plasmid (pAQ3::P(nir07)-fatB2 m -carB-entD-SpecR (SEQ ID NO. 31)) was transformed into the strain ADM and segregated in the presence of the antibiotic spectinomycin, resulting in strain ALK-C9. 
         [0157]    ALK-C9 ( FIG. 8A ) and ALK-C11 ( FIG. 8B ) were grown in JB3.0 media till OD 230 ˜3 at 37° C., 150 rpm and with 2% CO 2 , in the presence of 15 mM urea. The cells were spun down, resuspended in fresh media without urea and 8 mL hexadecane overlay was then added onto the 32 mL culture. Each day, 0.1 mL of the overlay was collected and analyzed by GC/FID equipped with an hp-5 ms column. An increasing amount of nonane was detected in the overlay for ALK-C9 ( FIG. 9 , circle), and an increasing amount of undecane was detected in the overlay for ALK-C11 ( FIG. 9 , triangle). Nonane and undecane are produced continuously by ALK-C9 and ALK-C11 from CO 2 . 
       Example 7 
     Biosynthesis of Tridecane and Pentadecane by  Synechococcus  Sp. PCC 7002 Strain Expressing Adm, tesA (Thioesterase), and carB/entD 
       [0158]    Carboxylic acid reductase (carB) (SEQ ID NO. 32) was PCR amplified from  Mycobacterium smegmatis  and verified by sequencing with multiple primers by Genewiz.  Cyanothece  sp. ATCC 51142 adm,  E. coli  tesA m  (where subscript “m” indicates mature protein, i.e. without leader sequence), and  E. coli  entD genes were codon-optimized for  E. coli  overexpression and synthesized by DNA 2.0 (Menlo Park, Calif.; SEQ ID NO. 33 and 34, respectively) with individual ribosome binding sites in front of each gene. All four genes were subcloned into a pUC19 vector containing a P(nir07) promoter, upstream/downstream homology regions, and a spectinomycin marker. The resulting plasmid (pAQ3::P(nir07)-adm-carB-tesA m -entD-SpecR (SEQ ID NO. 35)) was transformed into wild-type 7002 strain and segregated in the presence of the antibiotic spectinomycin resulting in strain ALK-C13C15. 
         [0159]    ALK-C13C15 of OD 730 ˜0.5 was grown in a shaker flask at 37° C., 150 rpm with 2% CO 2  in the presence of 2 mM urea in JB3.0 medium. After 48 h, 0.5 mL sample of the culture was collected and centrifuged for 5 min at 15,000 rpm. The cell pellet was extracted with acetone and analyzed by GC/FID equipped with an hp-5 ms column.  FIG. 10 . A control strain that did not express tesA m , carB, or entD proteins was treated similarly, and the sample was prepared and analyzed by the same method. 
         [0160]    The growth and alkane production of ALK-C13C15 was also analyzed over a ten day period of time.  FIG. 11  shows the growth curve of ALK-C13C15 over 10 days.  FIG. 12  shows the production curve of tridecane and pentadecane by ALK-C13C15 over 10 days. 
         [0161]    Nonane and undecane are produced continuously by ALK-C9 and ALK-C11 from in vivo using CO 2  and sunlight. 
       Example 8 
     A Pathway for the Enzymatic Synthesis of Short-Chain Alkanes 
       [0162]    Organisms are constructed which express both adm (alkanal deformylative monooxygenase) and a pathway leading to the formation of a short-chain aldehyde. Examples of such aldehyde-generating pathways are shown in Table 3. 
         [0000]    
       
         
               
             
               
               
               
               
             
           
               
                 TABLE 3 
               
             
             
               
                   
               
               
                 Pathways for production of an aldehyde and subsequent conversion 
               
               
                 to an alkane/alkene via alkanal deformylative monooxygenase. 
               
             
          
           
               
                   
                   
                 Resultant 
                   
               
               
                   
                 Pathway 
                 aldehyde 
                 Alkane product 
               
               
                   
                   
               
               
                   
                 pdc,  Zymomonas mobilis   
                 acetaldehyde 
                 methane 
               
               
                   
                 (EC 4.1.1.1) 
               
               
                   
                 2-ketoacid decarboxylase 
                 propanal 
                 ethane 
               
               
                   
                 (EC 4.1.1.72) 
                 isobutanal 
                 propane 
               
               
                   
                   
                 2-methyl-1-butanal 
                 butane 
               
               
                   
                   
                 butanal 
                 propane 
               
               
                   
                   
                 3-methyl-1-butanal 
                 isobutane 
               
               
                   
                   
                 2-phenylethanal 
                 toluene 
               
               
                   
                   
               
             
          
         
       
     
         [0163]    For example, an organism (e.g.,  cyanobacterium ) is engineered according to standard genetic engineering techniques to express Pdc from  Zymomonas mobilis  (SEQ ID NO: 46) and Adm from  N. punctiforme  (SEQ ID NO: 36). The Pdc polypeptide converts pyruvate to acetaldehyde. The Adm polypeptide converts acetaldehyde to the short-chain alkane, methane. The genes of the invention may be constructed synthetically or isolated by PCR. 
         [0164]    Alternatively, ketoacid decarboxylase and Adm are recombinantly expressed by the organism. The ketoacid decarboxylase is KivD from  Lactococcus lactis  subsp.  lactis  KF147 (SEQ ID NO: 43). Alternatively, the ketoacid decarboxylase is ARO10 from  Saccharomyces cerevisiae  S288c (SEQ ID NO: 44). 
         [0165]    The resulting organism comprises an operon coexpressing an adm gene and pdc and/or a 2-ketoacid decarboxylase gene. Cells will be cultured and the presence of the expected product in Table 3 will be measured by gas chromatography analysis. 
       Example 9 
     Purified ADM from  Nostoc punctiforme  PCC 73102 Deformylates Isovaleraldehyde and Forms Isobutane In Vitro 
       [0166]      N. punctiforme  PCC73102 adm was amplified from the codon-optimized gene obtained from DNA2.0 (Menlo Park, Calif.; SEQ ID NO. 37) by PCR using primers UN19 (5′-CAT CAC CAC AGC CAG GAT CCG ATG CAG CAA CTG ACC GAT CAA AGC AAA GAA CTG GAC TTC-3′) (SEQ ID NO: 40) and UN20 (5′-CGG CCC GCC AAG CTT TTA GGC ACC GAT CAG GCC ATA GGC GCT CAG ACG CAT GAT ATC-3′) (SEQ ID NO: 41), allowing the introduction of 5′ BamHI and 3′ HindIII restriction sites. The resulting PCR product was inserted into the  E. coli  vector pCDF-Duet1 (Merck; Darmstadt, Germany) by digestion with BamHI and HindIII and subsequent ligation. The resulting plasmid, pCDF-npu (SEQ ID NO. 42), containing N-terminal His 6 -tagged  N. punctiforme  adm, was transformed into  E. coli  strain BL21(DE3), which was subsequently grown with shaking in Luria-Bertani medium supplemented with 100 ng/mL of spectinomycin in a volume of 1 L to OD 600 =0.8 before induction with 0.25 mM IPTG for 4 hours in a 2-L shaker flask at 37° C. The ADM protein was purified by affinity chromatography using a Ni-NTA agarose (Qiagen; Valencia, Calif.) column, eluting the purified protein with a buffer solution of pH 7.5, which contained 100 mM HEPES, 10% glycerol and 250 mM imidazole. An SDS-PAGE gel of the collected fractions is shown in  FIG. 13 . 
         [0167]    The activity of the purified ADM was tested on various short-chain aldehydes: isobutyraldehyde, 2-methylbutyraldehyde, and 3-methylbutyraldehyde, among which the 3-methylbutyraldehyde (isovaleraldehyde) is converted to isobutane; whereas the other two showed no detectable deformylation to the corresponding alkane. The activity of purified ADM was also tested on butanal, valeraldehyde, and isovaleraldehyde, as shown in Table 4. The assay conditions were as follows: ˜0.2 mM  N. punctiforme  Adm (N-His 6 -tagged), 0.3 mM 1-methoxy-5-methylphenazinium methyl sulfate (Sigma-Aldrich; St. Louis, Mo.), 10 mM NADH (Sigma-Aldrich), 10 mM aldehyde (stock of 250 mM, dissolved in dimethyl sulfoxide), in a buffer solution containing 100 mM HEPES, 10% glycerol at pH 7.4. Each assay was run at 25° C. for 5 minutes, after which it was immediately analyzed by headspace gas chromatography using a 20-m HP-5MS column (Agilent Technologies; Santa Clara, Calif.). The column was kept at 40° C. for 3 min before being heated to 100° C. at 15 C.°/min. Species were identified according to retention time, compared to corresponding standards, which were purchased from Sigma-Aldrich. Results are shown in Table 4. The expression of ADM results in an increase in peak area for each product. 
         [0000]    
       
         
               
             
               
               
               
               
               
             
               
               
               
               
               
             
           
               
                 TABLE 4 
               
             
             
               
                   
               
               
                 Results of chromatagram assays. 
               
             
          
           
               
                   
                   
                 Product 
                   
                 Product 
               
               
                   
                   
                 retention 
                 Reaction 
                 peak area 
               
               
                 Substrate 
                 Product 
                 time (min) 
                 condition 
                 (arbitrary unit) 
               
               
                   
               
             
          
           
               
                 Butanal 
                 Propane 
                 1.33 
                 No ADM 
                 4.1 
               
               
                   
                   
                   
                 With ADM 
                 11.2 
               
               
                 Valeraldehyde 
                 Butane 
                 1.42 
                 No ADM 
                 2.5 
               
               
                   
                   
                   
                 With ADM 
                 32 
               
               
                 Isovaleraldehyde 
                 Isobutane 
                 1.36 
                 No ADM 
                 3.4 
               
               
                   
                   
                   
                 With ADM 
                 17.4 
               
               
                   
               
             
          
         
       
     
       Example 10 
     Biosynthesis of Undecane by  Synechococcus  Sp. PCC 7002 Strain Expressing Adm, Thioesterase and carB/entD with Secretion 
       [0168]    Carboxylic acid reductase (carB) (SEQ ID NO. 47) was PCR amplified from  Mycobacterium smegmatis  and verified by sequencing with multiple primers by Genewiz. Hexahistidine-tagged  Nostoc punctiforme  adm,  Umbellularia californicia  fatB m  (without leader sequence), and  E. coli  entD genes were codon-optimized for  E. coli  overexpression and synthesized by DNA2.0 (Menlo Park, Calif.; SEQ ID NO. 48, 49, and 50). The adm gene with an N-terminal hexahistidine tag was subcloned into a pUC19 vector with P(cpcB) promoter, upstream and downstream homologous regions, and a erythromycin marker. The resulting plasmid (pAQ4::P(cpcB)-Nhistag_adm(Npu)-ErmC (SEQ ID NO. 51)) was transformed into wild-type  Synechococcus  sp. PCC 7002 and segregated in the presence of erythromycin (which resulted in strain ADM). The fatB m , carB and entD genes were subcloned into a pUC19 vector containing a P(nir07) promoter, upstream and downstream homologous regions, and a spectinomycin marker. The resulting plasmid (pAQ3::P(nir07)-fatB m -carB-entD-SpecR (SEQ ID NO. 52)) was transformed into the strain ADM and segregated in the presence of the antibiotic spectinomycin, resulting in strain JCC6036. 
         [0169]    JCC6036 was grown up in JB3.0 media to OD 730 ˜3 at 37° C., 150 rpm and with 2% CO 2 , in the presence of 15 mM urea. The cells were spun down, resuspended in fresh JB3.0 media with 3 mM urea and a 6 mL pentadecane overlay was then added onto 30 mL culture. 0.06 mL of the overlay was collected everyday and analyzed by GC/FID equipped with an hp-5 ms column. An increased amount of undecane was detected in the overlay for JCC6036 ( FIG. 14 ). 
       Example 11 
     Feeding Decanoic Acid to Adm and carB/entD-Expressing  Synechococcus  Sp. PCC 7002 Strain Results in Detection of Corresponding Nonane with Secretion. His-Tagged Adm on pAQ3 Showed Significantly Higher Activity In Vivo 
       [0170]    Carboxylic acid reductase (carB) (SEQ ID NO. 53) was PCR amplified from  Mycobacterium smegmatis  and verified by sequencing with multiple primers by Genewiz. Hexahistidine-tagged  Nostoc punctiforme  adm and  E. coli  entD genes codon-optimized for  E. coli  overexpression were synthesized by DNA 2.0 (Menlo Park, Calif.; SEQ ID NO. 54 and 55). The adm gene was subcloned into a pUC19 vector with P(cpcB) promoter, upstream and downstream homologous regions of pAQ3 or pAQ4, and a spectinomycin marker. The resulting plasmids (pAQ3::P(cpcB)-Nhistag_adm(Npu)-SpecR (SEQ ID NO. 56) and pAQ4::P(cpcB)-Nhistag_adm(Npu)-EmrC (SEQ ID NO. 57)) were transformed into wild-type  Synechococcus  sp. PCC 7002 strain and segregated in the presence of spectinomycin (resulting in strains ADM3 and ADM4). The carB and entD genes were subcloned into a pUC19 vector containing a P(nir07) promoter, upstream and downstream homologous regions of pAQ7, and a kanamycin marker. The resulting plasmid (pAQ7::P(nir07)-carB-entD-KanR (SEQ ID NO. 58)) was transformed into strains ADM3 and ADM4 and segregated in the presence of the antibiotic spectinomycin (resulting in strains ADM3CARB and ADM4CARB). 
         [0171]    The ADM3CARB and ADM4CARB strains were grown in JB3.0 media to OD 730 ˜4 at 37° C., 150 rpm and with 2% CO 2 , in the presence of 15 mM urea. The cells were spun down, resuspended in fresh JB3.0 media without urea, and grown overnight to allow the expression of proteins regulated by the P(nir07) promoter. 1.5 mL pentadecane overlay was then added onto 6 mL of culture before 4 mM decanoic acid (500 mM stock, dissolved in 100% ethanol) was fed into the culture at the beginning. 0.08 mL of the overlay was collected at 1 and 2 hours after feeding and analyzed by GC/FID equipped with an hp-5 ms column. When fed with decanoic acid, nonane was produced in vivo by the strain ADM3CARB with an initial rate of ˜6 mg/L/h ( FIG. 15 ). 
         [0172]    A number of embodiments of the invention have been described. Nevertheless, it will be understood that various modifications may be made without departing from the spirit and scope of the invention. All publications, patents and other references mentioned herein are hereby incorporated by reference in their entirety. 
         [0000]    
       
         
               
               
               
             
               
               
               
             
           
               
                 TABLE 1 
               
               
                   
               
               
                 SEQ 
                   
                   
               
               
                 ID NO 
                 DESCRIPTION 
                 SEQUENCE 
               
               
                   
               
             
             
               
                   
               
             
          
           
               
                 1 
                 acrM (from 
                 ATGAATGCAAAACTGAAGAAATTGTTCCAGCAGAAAGTAGACGGCAAGACCATCATCGTGACCGGTGCAA 
               
               
                   
                 
                   Acinetobacter 
                 
                 GCAGCGGTATTGGCTTGACCGTGAGCAAATACCTGGCTCAGGCGGGTGCACACGTGCTGCTGCTGGCGCG 
               
               
                   
                 sp. M-1), 
                 TACGAAAGAGAAACTGGATGAGGTCAAGGCGGAGATTGAAGCGGAAGGCGGTAAGGCTACTGTTTTCCCG 
               
               
                   
                 codon- 
                 TGCGATTTGAATGACATGGAATCCATTGACGCAGTCAGCAAAGAGATCCTGGCAGCCGTTGATCATATCG 
               
               
                   
                 optimized for 
                 ACATTCTGGTGAATAACGCGGGTCGCAGCATCCGTCGCGCGGTCCACGAAAGCGTGGATCGCTTCCATGA 
               
               
                   
                 
                   E. coli 
                 
                 CTTTGAGCGTACCATGCAACTGAATTACTTCGGTGCCGTTCGTCTGGTCCTGAATGTTCTGCCGCACATG 
               
               
                   
                   
                 ATGCAGCGCAAAGATGGCCAAATCATTAACATTAGCAGCATTGGCGTTTTGGCGAACGCGACGCGTTTCA 
               
               
                   
                   
                 GCGCGTATGTGGCGAGCAAGGCTGCACTGGATGCCTTCTCCCGTTGTCTGAGCGCCGAGGTCCATTCGCA 
               
               
                   
                   
                 CAAGATTGCGATTACCTCTATCTATATGCCGCTGGTTCGTACCCCGATGATTGCGCCGACGAAGATCTAC 
               
               
                   
                   
                 AAGTATGTCCCAACGTTGTCCCCGGAAGAGGCGGCTGACCTGATTGCTTATGCGATCGTTAAACGTCCGA 
               
               
                   
                   
                 AAAAGATCGCCACCAATCTGGGTCGCCTGGCAAGCATCACCTACGCGATTGCCCCGGACATCAACAACAT 
               
               
                   
                   
                 CCTGATGAGCATCGGCTTTAACCTGTTTCCGTCTAGCACGGCGAGCGTGGGTGAGCAAGAAAAGCTGAAC 
               
               
                   
                   
                 CTGATTCAACGTGCCTACGCACGTCTGTTTCCTGGTGAACACTGGTAA 
               
               
                   
               
               
                 2 
                 Plasmid 
                 TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACC 
               
               
                   
                 pET28a-acrM 
                 GCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCG 
               
               
                   
                   
                 GCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGA 
               
               
                   
                   
                 CCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCT 
               
               
                   
                   
                 TTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT 
               
               
                   
                   
                 CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTA 
               
               
                   
                   
                 ACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAAT 
               
               
                   
                   
                 GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCT 
               
               
                   
                   
                 TAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTT 
               
               
                   
                   
                 GAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA 
               
               
                   
                   
                 TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTA 
               
               
                   
                   
                 TCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCC 
               
               
                   
                   
                 AGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCAT 
               
               
                   
                   
                 TCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAA 
               
               
                   
                   
                 TGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA 
               
               
                   
                   
                 CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATG 
               
               
                   
                   
                 CTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTG 
               
               
                   
                   
                 GCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTG 
               
               
                   
                   
                 TCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATT 
               
               
                   
                   
                 TAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG 
               
               
                   
                   
                 TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGAC 
               
               
                   
                   
                 CCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAA 
               
               
                   
                   
                 AAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAC 
               
               
                   
                   
                 TGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAG 
               
               
                   
                   
                 AACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA 
               
               
                   
                   
                 AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGG 
               
               
                   
                   
                 GGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTA 
               
               
                   
                   
                 TGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAG 
               
               
                   
                   
                 GAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCT 
               
               
                   
                   
                 CTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG 
               
               
                   
                   
                 GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATT 
               
               
                   
                   
                 CTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAG 
               
               
                   
                   
                 CGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATT 
               
               
                   
                   
                 TCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACT 
               
               
                   
                   
                 CCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC 
               
               
                   
                   
                 GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAG 
               
               
                   
                   
                 GTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGAT 
               
               
                   
                   
                 TCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTC 
               
               
                   
                   
                 TGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATT 
               
               
                   
                   
                 TCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA 
               
               
                   
                   
                 CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAA 
               
               
                   
                   
                 TCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCC 
               
               
                   
                   
                 TGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGG 
               
               
                   
                   
                 AAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCT 
               
               
                   
                   
                 CGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG 
               
               
                   
                   
                 GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTG 
               
               
                   
                   
                 GTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGA 
               
               
                   
                   
                 TCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTAC 
               
               
                   
                   
                 GAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAG 
               
               
                   
                   
                 CTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT 
               
               
                   
                   
                 TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGG 
               
               
                   
                   
                 CCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGG 
               
               
                   
                   
                 CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC 
               
               
                   
                   
                 AGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGT 
               
               
                   
                   
                 ATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC 
               
               
                   
                   
                 CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGA 
               
               
                   
                   
                 AAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATT 
               
               
                   
                   
                 TATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTG 
               
               
                   
                   
                 GTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTG 
               
               
                   
                   
                 ATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG 
               
               
                   
                   
                 CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGC 
               
               
                   
                   
                 CGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCG 
               
               
                   
                   
                 CGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCA 
               
               
                   
                   
                 GCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGC 
               
               
                   
                   
                 TTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA 
               
               
                   
                   
                 GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCT 
               
               
                   
                   
                 CTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCT 
               
               
                   
                   
                 CTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGC 
               
               
                   
                   
                 AAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACG 
               
               
                   
                   
                 CCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGG 
               
               
                   
                   
                 CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTC 
               
               
                   
                   
                 GATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAA 
               
               
                   
                   
                 TTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCC 
               
               
                   
                   
                 TGGTGCCGCGCGGCAGCCATATGAATGCAAAACTGAAGAAATTGTTCCAGCAGAAAGTAGACGGCAAGAC 
               
               
                   
                   
                 CATCATCGTGACCGGTGCAAGCAGCGGTATTGGCTTGACCGTGAGCAAATACCTGGCTCAGGCGGGTGCA 
               
               
                   
                   
                 CACGTGCTGCTGCTGGCGCGTACGAAAGAGAAACTGGATGAGGTCAAGGCGGAGATTGAAGCGGAAGGCG 
               
               
                   
                   
                 GTAAGGCTACTGTTTTCCCGTGCGATTTGAATGACATGGAATCCATTGACGCAGTCAGCAAAGAGATCCT 
               
               
                   
                   
                 GGCAGCCGTTGATCATATCGACATTCTGGTGAATAACGCGGGTCGCAGCATCCGTCGCGCGGTCCACGAA 
               
               
                   
                   
                 AGCGTGGATCGCTTCCATGACTTTGAGCGTACCATGCAACTGAATTACTTCGGTGCCGTTCGTCTGGTCC 
               
               
                   
                   
                 TGAATGTTCTGCCGCACATGATGCAGCGCAAAGATGGCCAAATCATTAACATTAGCAGCATTGGCGTTTT 
               
               
                   
                   
                 GGCGAACGCGACGCGTTTCAGCGCGTATGTGGCGAGCAAGGCTGCACTGGATGCCTTCTCCCGTTGTCTG 
               
               
                   
                   
                 AGCGCCGAGGTCCATTCGCACAAGATTGCGATTACCTCTATCTATATGCCGCTGGTTCGTACCCCGATGA 
               
               
                   
                   
                 TTGCGCCGACGAAGATCTACAAGTATGTCCCAACGTTGTCCCCGGAAGAGGCGGCTGACCTGATTGCTTA 
               
               
                   
                   
                 TGCGATCGTTAAACGTCCGAAAAAGATCGCCACCAATCTGGGTCGCCTGGCAAGCATCACCTACGCGATT 
               
               
                   
                   
                 GCCCCGGACATCAACAACATCCTGATGAGCATCGGCTTTAACCTGTTTCCGTCTAGCACGGCGAGCGTGG 
               
               
                   
                   
                 GTGAGCAAGAAAAGCTGAACCTGATTCAACGTGCCTACGCACGTCTGTTTCCTGGTGAACACTGGTAAGA 
               
               
                   
                   
                 ATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGC 
               
               
                   
                   
                 TAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGG 
               
               
                   
                   
                 GCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT 
               
               
                   
               
               
                 3 
                 carboxylic 
                 GAGCTCGAGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCAC 
               
               
                   
                 acid 
                 TCGACGACGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGC 
               
               
                   
                 reductase 
                 ACCGTTGCCCGCCGTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTG 
               
               
                   
                 amplified 
                 TTCACCGGCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGC 
               
               
                   
                 from 
                 GCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGC 
               
               
                   
                 
                   Mycobacterium 
                 
                 GGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGT 
               
               
                   
                   smegmatis . 
                 TTCGCGAGTCCCGATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGC 
               
               
                   
                   
                 AGCACAACGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAG 
               
               
                   
                   
                 CGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTC 
               
               
                   
                   
                 GACCATCACCCCGAGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGG 
               
               
                   
                   
                 GCATCGCCGTCACCACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACAC 
               
               
                   
                   
                 CGCCGACCATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCG 
               
               
                   
                   
                 ATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCA 
               
               
                   
                   
                 TCAACGTCAACTTCATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGG 
               
               
                   
                   
                 TGGAACCAGTTACTTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCG 
               
               
                   
                   
                 ACCGAACTCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCC 
               
               
                   
                   
                 TGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCT 
               
               
                   
                   
                 CGGCGGACGCGTGATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGAC 
               
               
                   
                   
                 ATCACCCTGGGCGCACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTG 
               
               
                   
                   
                 TGATCGTGCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGA 
               
               
                   
                   
                 CAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCC 
               
               
                   
                   
                 GAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCAC 
               
               
                   
                   
                 CCGACCACCTGGTGTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGT 
               
               
                   
                   
                 CGCCAACCTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAG 
               
               
                   
                   
                 CGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCA 
               
               
                   
                   
                 AGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGC 
               
               
                   
                   
                 CGATTTCATCGTCGAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTG 
               
               
                   
                   
                 CGGCCCAACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGG 
               
               
                   
                   
                 CCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGC 
               
               
                   
                   
                 TGCCACGATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCC 
               
               
                   
                   
                 CTGTCGGCGCTGACACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCG 
               
               
                   
                   
                 TGAACCCGGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAG 
               
               
                   
                   
                 GCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAG 
               
               
                   
                   
                 TTCATCGACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGT 
               
               
                   
                   
                 TGCTCTCGGGCGCCAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGT 
               
               
                   
                   
                 CGGCGGCACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCC 
               
               
                   
                   
                 TACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCG 
               
               
                   
                   
                 GTGACATCGGCGACCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCT 
               
               
                   
                   
                 GGTGGTGCATCCGGCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTG 
               
               
                   
                   
                 GGCACGGCCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGT 
               
               
                   
                   
                 CGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCT 
               
               
                   
                   
                 CGACGGCGGATACGCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCAC 
               
               
                   
                   
                 GATCTGTGCGGGCTGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTC 
               
               
                   
                   
                 AGGTCAACGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTC 
               
               
                   
                   
                 GTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAG 
               
               
                   
                   
                 GCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACG 
               
               
                   
                   
                 ACGGGATCTCCCTGGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGA 
               
               
                   
                   
                 CTACGACGACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACC 
               
               
                   
                   
                 GTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGG 
               
               
                   
                   
                 TGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGAT 
               
               
                   
                   
                 CGACAAGTACATACGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACC 
               
               
                   
               
               
                 4 
                 codon- 
                 CATATGCAAGAACTGGCCCTGAGAAGCGAGCTGGACTTCAATAGCGAAACCTATAAAGATGCGTATAGCC 
               
               
                   
                 optimized 
                 GTATTAACGCCATTGTGATCGAAGGCGAGCAAGAAGCATACCAAAACTACCTGGACATGGCGCAACTGCT 
               
               
                   
                 
                   Cyanothece 
                 
                 GCCGGAGGACGAGGCTGAGCTGATTCGTTTGAGCAAGATGGAGAACCGTCACAAAAAGGGTTTTCAAGCG 
               
               
                   
                   adm . 
                 TGCGGCAAGAACCTCAATGTGACTCCGGATATGGATTATGCACAGCAGTTCTTTGCGGAGCTGCACGGCA 
               
               
                   
                   
                 ATTTTCAGAAGGCTAAAGCCGAGGGTAAGATTGTTACCTGCCTGCTCATCCAAAGCCTGATCATCGAGGC 
               
               
                   
                   
                 GTTTGCGATTGCAGCCTACAACATTTACATTCCAGTGGCTGATCCGTTTGCACGTAAAATCACCGAGGGT 
               
               
                   
                   
                 GTCGTCAAGGATGAGTATACCCACCTGAATTTCGGCGAAGTTTGGTTGAAGGAACATTTTGAAGCAAGCA 
               
               
                   
                   
                 AGGCGGAGTTGGAGGACGCCAACAAAGAGAACTTACCGCTGGTCTGGCAGATGTTGAACCAGGTCGAAAA 
               
               
                   
                   
                 GGATGCCGAAGTGCTGGGTATGGAGAAAGAGGCTCTGGTGGAGGACTTTATGATTAGCTATGGTGAGGCA 
               
               
                   
                   
                 CTGAGCAACATCGGCTTTTCTACGAGAGAAATCATGAAGATGAGCGCGTACGGTCTGCGTGCAGCATAAG 
               
               
                   
                   
                 AGCTC 
               
               
                   
               
               
                 5 
                 codon- 
                 GAGCTCGAGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCAC 
               
               
                   
                 optimized  E.   
                 TCGACGACGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGC 
               
               
                   
                   coli  tesA and 
                 ACCGTTGCCCGCCGTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTG 
               
               
                   
                   E. coli  entD 
                 TTCACCGGCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGC 
               
               
                   
                 genes. 
                 GCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGC 
               
               
                   
                   
                 GGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGT 
               
               
                   
                   
                 TTCGCGAGTCCCGATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGC 
               
               
                   
                   
                 AGCACAACGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAG 
               
               
                   
                   
                 CGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTC 
               
               
                   
                   
                 GACCATCACCCCGAGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGG 
               
               
                   
                   
                 GCATCGCCGTCACCACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACAC 
               
               
                   
                   
                 CGCCGACCATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCG 
               
               
                   
                   
                 ATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCA 
               
               
                   
                   
                 TCAACGTCAACTTCATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGG 
               
               
                   
                   
                 TGGAACCAGTTACTTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCG 
               
               
                   
                   
                 ACCGAACTCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCC 
               
               
                   
                   
                 TGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCT 
               
               
                   
                   
                 CGGCGGACGCGTGATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGAC 
               
               
                   
                   
                 ATCACCCTGGGCGCACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTG 
               
               
                   
                   
                 TGATCGTGCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGA 
               
               
                   
                   
                 CAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCC 
               
               
                   
                   
                 GAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCAC 
               
               
                   
                   
                 CCGACCACCTGGTGTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGT 
               
               
                   
                   
                 CGCCAACCTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAG 
               
               
                   
                   
                 CGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCA 
               
               
                   
                   
                 AGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGC 
               
               
                   
                   
                 CGATTTCATCGTCGAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTG 
               
               
                   
                   
                 CGGCCCAACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGG 
               
               
                   
                   
                 CCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGC 
               
               
                   
                   
                 TGCCACGATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCC 
               
               
                   
                   
                 CTGTCGGCGCTGACACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCG 
               
               
                   
                   
                 TGAACCCGGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAG 
               
               
                   
                   
                 GCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAG 
               
               
                   
                   
                 TTCATCGACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGT 
               
               
                   
                   
                 TGCTCTCGGGCGCCAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGT 
               
               
                   
                   
                 CGGCGGCACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCC 
               
               
                   
                   
                 TACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCG 
               
               
                   
                   
                 GTGACATCGGCGACCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCT 
               
               
                   
                   
                 GGTGGTGCATCCGGCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTG 
               
               
                   
                   
                 GGCACGGCCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGT 
               
               
                   
                   
                 CGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCT 
               
               
                   
                   
                 CGACGGCGGATACGCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCAC 
               
               
                   
                   
                 GATCTGTGCGGGCTGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTC 
               
               
                   
                   
                 AGGTCAACGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTC 
               
               
                   
                   
                 GTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAG 
               
               
                   
                   
                 GCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACG 
               
               
                   
                   
                 ACGGGATCTCCCTGGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGA 
               
               
                   
                   
                 CTACGACGACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACC 
               
               
                   
                   
                 GTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGG 
               
               
                   
                   
                 TGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGAT 
               
               
                   
                   
                 CGACAAGTACATACGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCAGGAGGTTTTTACAATGGCTG 
               
               
                   
                   
                 ATACTTTGTTGATTTTGGGTGATTCTCTCTCTGCAGGCTACCGTATGTCCGCGAGCGCGGCATGGCCGGC 
               
               
                   
                   
                 TCTGCTGAACGATAAGTGGCAGAGCAAGACCAGCGTGGTCAATGCGAGCATCAGCGGCGATACCAGCCAG 
               
               
                   
                   
                 CAGGGTCTGGCACGTCTGCCAGCGCTGCTGAAGCAACACCAGCCGCGTTGGGTGCTGGTTGAACTGGGCG 
               
               
                   
                   
                 GCAATGACGGTCTGCGTGGTTTTCAGCCGCAGCAGACCGAACAAACGTTGCGTCAGATTCTGCAGGACGT 
               
               
                   
                   
                 CAAGGCGGCTAACGCGGAACCGCTGCTGATGCAAATTCGCCTGCCGGCGAATTATGGTCGTCGTTACAAC 
               
               
                   
                   
                 GAGGCTTTCAGCGCCATTTATCCTAAACTGGCTAAAGAGTTTGACGTGCCGCTGCTGCCGTTCTTCATGG 
               
               
                   
                   
                 AAGAGGTCTACCTGAAACCGCAATGGATGCAAGACGACGGTATTCATCCGAATCGTGATGCACAACCTTT 
               
               
                   
                   
                 CATCGCGGATTGGATGGCGAAGCAATTGCAACCGCTGGTGAACCATGACTCGTAAAAGCTTGTTGCTGCA 
               
               
                   
                   
                 TGCAGGAGGTTTTTACAATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGT 
               
               
                   
                   
                 CGAATTTGATCCGGCGAACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCAC 
               
               
                   
                   
                 GCAGGCCGTAAGCGTAAAACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACG 
               
               
                   
                   
                 GCTACAAATGCGTGCCGGCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCAT 
               
               
                   
                   
                 CTCCCACTGCGGTACTACCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATA 
               
               
                   
                   
                 TTCTCTGTCCAGACGGCACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGG 
               
               
                   
                   
                 ACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTC 
               
               
                   
                   
                 CGAGATCCAAACCGATGCGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATT 
               
               
                   
                   
                 CACCGTGAGAATGAGATGTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGC 
               
               
                   
                   
                 ACGACTGAGAATTC 
               
               
                   
               
               
                 6 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTA 
               
               
                   
                 pAQ3::Pnir07_ 
                 CGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 adm_carB_tesA_ 
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA 
               
               
                   
                 entD_SpecR. 
                 GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                   
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT 
               
               
                   
                   
                 GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG 
               
               
                   
                   
                 GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT 
               
               
                   
                   
                 CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC 
               
               
                   
                   
                 GGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAAC 
               
               
                   
                   
                 TGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGT 
               
               
                   
                   
                 GTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC 
               
               
                   
                   
                 GCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATAAATGAGAAAAAAGC 
               
               
                   
                   
                 AACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAACACCTATAATTAAACTATTCATCTATTA 
               
               
                   
                   
                 TTTATGATTTTTTGTATATACAATATTTCTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATA 
               
               
                   
                   
                 ATAAAGGGAAAATCAGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCGCTGAACCTGCAGGC 
               
               
                   
                   
                 GAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAACCCGTCGCCATTGACCCAGAACCGCGCA 
               
               
                   
                   
                 AAGAACGGGAAAAAATTGATCTCGATCTGGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAA 
               
               
                   
                   
                 AGTGAAGTTAGCCGATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGACCTCTTCAAGGATG 
               
               
                   
                   
                 AAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTAAATCGTTCCTGACCGGACTCGCGGAAAA 
               
               
                   
                   
                 AGGCTACGGTGACACCCAACTGAAGGCGATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGAT 
               
               
                   
                   
                 GTCCTGACTTGGGTTGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGACCAATACATTCGAGA 
               
               
                   
                   
                 AGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCATCGAAATCAAATACCAAACCGTTAATGAA 
               
               
                   
                   
                 GGTTTAGTGATCTTGGGTCAGGATATCGGTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAG 
               
               
                   
                   
                 ATGTGGCATAAAAAAGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCAAAAAGTATCAATGA 
               
               
                   
                   
                 TTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGCATGATTTACAAAAAGTTGTAGTTTCTGT 
               
               
                   
                   
                 TACCAATTGCGAATCGAGAACTGCCTAATCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATG 
               
               
                   
                   
                 CAAGAACTGGCCCTGAGAAGCGAGCTGGACTTCAATAGCGAAACCTATAAAGATGCGTATAGCCGTATTA 
               
               
                   
                   
                 ACGCCATTGTGATCGAAGGCGAGCAAGAAGCATACCAAAACTACCTGGACATGGCGCAACTGCTGCCGGA 
               
               
                   
                   
                 GGACGAGGCTGAGCTGATTCGTTTGAGCAAGATGGAGAACCGTCACAAAAAGGGTTTTCAAGCGTGCGGC 
               
               
                   
                   
                 AAGAACCTCAATGTGACTCCGGATATGGATTATGCACAGCAGTTCTTTGCGGAGCTGCACGGCAATTTTC 
               
               
                   
                   
                 AGAAGGCTAAAGCCGAGGGTAAGATTGTTACCTGCCTGCTCATCCAAAGCCTGATCATCGAGGCGTTTGC 
               
               
                   
                   
                 GATTGCAGCCTACAACATTTACATTCCAGTGGCTGATCCGTTTGCACGTAAAATCACCGAGGGTGTCGTC 
               
               
                   
                   
                 AAGGATGAGTATACCCACCTGAATTTCGGCGAAGTTTGGTTGAAGGAACATTTTGAAGCAAGCAAGGCGG 
               
               
                   
                   
                 AGTTGGAGGACGCCAACAAAGAGAACTTACCGCTGGTCTGGCAGATGTTGAACCAGGTCGAAAAGGATGC 
               
               
                   
                   
                 CGAAGTGCTGGGTATGGAGAAAGAGGCTCTGGTGGAGGACTTTATGATTAGCTATGGTGAGGCACTGAGC 
               
               
                   
                   
                 AACATCGGCTTTTCTACGAGAGAAATCATGAAGATGAGCGCGTACGGTCTGCGTGCAGCATAAGAGCTCG 
               
               
                   
                   
                 AGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGA 
               
               
                   
                   
                 CGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTG 
               
               
                   
                   
                 CCCGCCGTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCG 
               
               
                   
                   
                 GCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGT 
               
               
                   
                   
                 GACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCC 
               
               
                   
                   
                 GCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGA 
               
               
                   
                   
                 GTCCCGATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAA 
               
               
                   
                   
                 CGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAA 
               
               
                   
                   
                 TACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATC 
               
               
                   
                   
                 ACCCCGAGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGC 
               
               
                   
                   
                 CGTCACCACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGAC 
               
               
                   
                   
                 CATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACA 
               
               
                   
                   
                 CCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGT 
               
               
                   
                   
                 CAACTTCATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACC 
               
               
                   
                   
                 AGTTACTTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAAC 
               
               
                   
                   
                 TCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCAC 
               
               
                   
                   
                 GCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGA 
               
               
                   
                   
                 CGCGTGATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCC 
               
               
                   
                   
                 TGGGCGCACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGT 
               
               
                   
                   
                 GCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCC 
               
               
                   
                   
                 TACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCA 
               
               
                   
                   
                 CCGCGAGCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCA 
               
               
                   
                   
                 CCTGGTGTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAAC 
               
               
                   
                   
                 CTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTT 
               
               
                   
                   
                 TCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGC 
               
               
                   
                   
                 GCTGGCCGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTC 
               
               
                   
                   
                 ATCGTCGAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCA 
               
               
                   
                   
                 ACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCA 
               
               
                   
                   
                 GTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACG 
               
               
                   
                   
                 ATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGG 
               
               
                   
                   
                 CGCTGACACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCC 
               
               
                   
                   
                 GGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGT 
               
               
                   
                   
                 TTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCG 
               
               
                   
                   
                 ACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTC 
               
               
                   
                   
                 GGGCGCCAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGC 
               
               
                   
                   
                 ACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACA 
               
               
                   
                   
                 CCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACAT 
               
               
                   
                   
                 CGGCGACCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTG 
               
               
                   
                   
                 CATCCGGCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGG 
               
               
                   
                   
                 CCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGC 
               
               
                   
                   
                 CATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGC 
               
               
                   
                   
                 GGATACGCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGT 
               
               
                   
                   
                 GCGGGCTGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAA 
               
               
                   
                   
                 CGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTAC 
               
               
                   
                   
                 ATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCA 
               
               
                   
                   
                 CGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGAT 
               
               
                   
                   
                 CTCCCTGGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGAC 
               
               
                   
                   
                 GACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGC 
               
               
                   
                   
                 CGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCA 
               
               
                   
                   
                 CGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAG 
               
               
                   
                   
                 TACATACGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCAGGAGGTTTTTACAATGGCTGATACTTT 
               
               
                   
                   
                 GTTGATTTTGGGTGATTCTCTCTCTGCAGGCTACCGTATGTCCGCGAGCGCGGCATGGCCGGCTCTGCTG 
               
               
                   
                   
                 AACGATAAGTGGCAGAGCAAGACCAGCGTGGTCAATGCGAGCATCAGCGGCGATACCAGCCAGCAGGGTC 
               
               
                   
                   
                 TGGCACGTCTGCCAGCGCTGCTGAAGCAACACCAGCCGCGTTGGGTGCTGGTTGAACTGGGCGGCAATGA 
               
               
                   
                   
                 CGGTCTGCGTGGTTTTCAGCCGCAGCAGACCGAACAAACGTTGCGTCAGATTCTGCAGGACGTCAAGGCG 
               
               
                   
                   
                 GCTAACGCGGAACCGCTGCTGATGCAAATTCGCCTGCCGGCGAATTATGGTCGTCGTTACAACGAGGCTT 
               
               
                   
                   
                 TCAGCGCCATTTATCCTAAACTGGCTAAAGAGTTTGACGTGCCGCTGCTGCCGTTCTTCATGGAAGAGGT 
               
               
                   
                   
                 CTACCTGAAACCGCAATGGATGCAAGACGACGGTATTCATCCGAATCGTGATGCACAACCTTTCATCGCG 
               
               
                   
                   
                 GATTGGATGGCGAAGCAATTGCAACCGCTGGTGAACCATGACTCGTAAAAGCTTGTTGCTGCATGCAGGA 
               
               
                   
                   
                 GGTTTTTACAATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTT 
               
               
                   
                   
                 GATCCGGCGAACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCC 
               
               
                   
                   
                 GTAAGCGTAAAACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAA 
               
               
                   
                   
                 ATGCGTGCCGGCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCAC 
               
               
                   
                   
                 TGCGGTACTACCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTG 
               
               
                   
                   
                 TCCAGACGGCACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGG 
               
               
                   
                   
                 TCTGGCGTTCAGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATC 
               
               
                   
                   
                 CAAACCGATGCGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTG 
               
               
                   
                   
                 AGAATGAGATGTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTG 
               
               
                   
                   
                 AGAATTCGGTTTTCCGTCCTGTCTTGATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTT 
               
               
                   
                   
                 GTTTTTGTTTATTGCAAAAACAAAAAATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAA 
               
               
                   
                   
                 ATAATTTGCCATTTACTAGTTTTTAATTAACCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGT 
               
               
                   
                   
                 GGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAA 
               
               
                   
                   
                 GCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCC 
               
               
                   
                   
                 CTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGT 
               
               
                   
                   
                 TGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGC 
               
               
                   
                   
                 GGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGC 
               
               
                   
                   
                 GAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGA 
               
               
                   
                   
                 AGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGA 
               
               
                   
                   
                 GAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCT 
               
               
                   
                   
                 TGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGT 
               
               
                   
                   
                 TCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCT 
               
               
                   
                   
                 GGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGC 
               
               
                   
                   
                 CGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGC 
               
               
                   
                   
                 TAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTC 
               
               
                   
                   
                 CACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAATGTCTAACAATTCGTTCAAGCCGACGCC 
               
               
                   
                   
                 GCTTCGCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAG 
               
               
                   
                   
                 GTCAAGTCTGCTTTTATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAG 
               
               
                   
                   
                 GCGCGCCACGAGAAAGAGTTATGACAAATTAAAATTCTGACTCTTAGATTATTTCCAGAGAGGCTGATTT 
               
               
                   
                   
                 TCCCAATCTTTGGGAAAGCCTAAGTTTTTAGATTCTATTTCTGGATACATCTCAAAAGTTCTTTTTAAAT 
               
               
                   
                   
                 GCTGTGCAAAATTATGCTCTGGTTTAATTCTGTCTAAGAGATACTGAATACAACATAAGCCAGTGAAAAT 
               
               
                   
                   
                 TTTACGGCTGTTTCTTTGATTAATATCCTCCAATACTTCTCTAGAGAGCCATTTTCCTTTTAACCTATCA 
               
               
                   
                   
                 GGCAATTTAGGTGATTCTCCTAGCTGTATATTCCAGAGCCTTGAATGATGAGCGCAAATATTTCTAATAT 
               
               
                   
                   
                 GCGACAAAGACCGTAACCAAGATATAAAAAACTTGTTAGGTAATTGGAAATGAGTATGTATTTTTTGTCG 
               
               
                   
                   
                 TGTCTTAGATGGTAATAAATTTGTGTACATTCTAGATAACTGCCCAAAGGCGATTATCTCCAAAGCCATA 
               
               
                   
                   
                 TATGACGGCGGTAGTAGAGGATTTGTGTACTTGTTTCGATAATGCCCGATAAATTCTTCTACTTTTTTAG 
               
               
                   
                   
                 ATTGGCAATATTGAGTAATCGAATCGATTAATTCTTGATGCTTCCCAGTGTCATAAAATAAACTTTTATT 
               
               
                   
                   
                 CAGATACCAATGAGGATCATAATCATGGGAGTAGTGATAAATCATTTGAGTTCTGACTGCTACTTCTATC 
               
               
                   
                   
                 GACTCCGTAGCATTAAAAATAAGCATTCTCAAGGATTTATCAAACTTGTATAGATTTGGCCGGCCCGTCA 
               
               
                   
                   
                 AAAGGGCGACACCCCATAATTAGCCCGGGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTG 
               
               
                   
                   
                 ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTC 
               
               
                   
                   
                 TGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAACAAGGGGTGTTATGAGCC 
               
               
                   
                   
                 ATATTCAGGTATAAATGGGCTCGCGATAATGTTCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATT 
               
               
                   
                   
                 TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAAT 
               
               
                   
                   
                 AATATTGAAAAAGGAAGAATATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT 
               
               
                   
                   
                 TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA 
               
               
                   
                   
                 CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTT 
               
               
                   
                   
                 TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGA 
               
               
                   
                   
                 GCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT 
               
               
                   
                   
                 CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA 
               
               
                   
                   
                 ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGT 
               
               
                   
                   
                 AACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG 
               
               
                   
                   
                 CCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAAC 
               
               
                   
                   
                 AATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTG 
               
               
                   
                   
                 GTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGAT 
               
               
                   
                   
                 GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGAC 
               
               
                   
                   
                 AGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGT 
               
               
                   
               
               
                 7 
                 codon- 
                 GGTACCAGGAGGTTTTTACATGGACCGTAAAAGCAAGCGTCCGGACATGCTGGTTGATTCCTTTGGTCTG 
               
               
                   
                 optimized 
                 GAAAGCACCGTGCAGGACGGTCTGGTTTTCCGTCAGTCTTTCTCCATTCGTAGCTATGAGATTGGTACTG 
               
               
                   
                 
                   Cuphea 
                 
                 ATCGTACCGCCTCTATCGAAACCCTGATGAATCACCTGCAAGAAACCTCTCTGAACCATTGTAAGTCTAC 
               
               
                   
                 
                   hookeriana 
                 
                 TGGCATCCTGCTGGACGGTTTCGGTCGTACCCTGGAGATGTGCAAACGCGACCTGATTTGGGTAGTGATC 
               
               
                   
                 leaderless 
                 AAAATGCAGATCAAAGTTAACCGTTATCCGGCATGGGGTGATACCGTTGAAATCAACACCCGCTTTTCTC 
               
               
                   
                 fatB2 gene. 
                 GTCTGGGCAAAATCGGTATGGGCCGTGACTGGCTGATCTCTGACTGTAACACTGGTGAAATTCTGGTTCG 
               
               
                   
                   
                 TGCTACTAGCGCATACGCGATGATGAACCAGAAAACCCGTCGCCTGAGCAAGCTGCCGTACGAGGTCCAC 
               
               
                   
                   
                 CAGGAGATTGTTCCGCTGTTTGTAGACAGCCCAGTGATTGAGGATTCTGACCTGAAAGTGCATAAATTCA 
               
               
                   
                   
                 AAGTGAAGACCGGTGACAGCATCCAAAAAGGCCTGACCCCAGGTTGGAACGATCTGGACGTTAACCAGCA 
               
               
                   
                   
                 CGTTTCCAACGTGAAGTATATCGGTTGGATTCTGGAGAGCATGCCGACCGAGGTCCTGGAAACCCAGGAG 
               
               
                   
                   
                 CTGTGTTCCCTGGCGCTGGAGTACCGCCGTGAGTGCGGCCGTGACAGCGTGCTGGAGTCTGTGACCGCTA 
               
               
                   
                   
                 TGGACCCAAGCAAAGTTGGTGTTCGTAGCCAGTACCAGCACCTGCTGCGTCTGGAAGACGGTACTGCTAT 
               
               
                   
                   
                 CGTGAACGGTGCAACTGAATGGCGTCCTAAAAACGCGGGTGCAAACGGTGCTATCAGCACCGGTAAAACC 
               
               
                   
                   
                 TCTAACGGTAACTCCGTGAGCTAAAAGCTT 
               
               
                   
               
               
                 8 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTA 
               
               
                   
                 pAQ3::P(nir07)_ 
                 CGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 adm_carB_ 
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA 
               
               
                   
                 fatB2_entD_ 
                 GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                 SpecR. 
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT 
               
               
                   
                   
                 GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG 
               
               
                   
                   
                 GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT 
               
               
                   
                   
                 CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC 
               
               
                   
                   
                 GGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAAC 
               
               
                   
                   
                 TGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGT 
               
               
                   
                   
                 GTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC 
               
               
                   
                   
                 GCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATAAATGAGAAAAAAGC 
               
               
                   
                   
                 AACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAACACCTATAATTAAACTATTCATCTATTA 
               
               
                   
                   
                 TTTATGATTTTTTGTATATACAATATTTCTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATA 
               
               
                   
                   
                 ATAAAGGGAAAATCAGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCGCTGAACCTGCAGGC 
               
               
                   
                   
                 GAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAACCCGTCGCCATTGACCCAGAACCGCGCA 
               
               
                   
                   
                 AAGAACGGGAAAAAATTGATCTCGATCTGGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAA 
               
               
                   
                   
                 AGTGAAGTTAGCCGATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGACCTCTTCAAGGATG 
               
               
                   
                   
                 AAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTAAATCGTTCCTGACCGGACTCGCGGAAAA 
               
               
                   
                   
                 AGGCTACGGTGACACCCAACTGAAGGCGATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGAT 
               
               
                   
                   
                 GTCCTGACTTGGGTTGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGACCAATACATTCGAGA 
               
               
                   
                   
                 AGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCATCGAAATCAAATACCAAACCGTTAATGAA 
               
               
                   
                   
                 GGTTTAGTGATCTTGGGTCAGGATATCGGTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAG 
               
               
                   
                   
                 ATGTGGCATAAAAAAGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCAAAAAGTATCAATGA 
               
               
                   
                   
                 TTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGCATGATTTACAAAAAGTTGTAGTTTCTGT 
               
               
                   
                   
                 TACCAATTGCGAATCGAGAACTGCCTAATCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATG 
               
               
                   
                   
                 CAAGAACTGGCCCTGAGAAGCGAGCTGGACTTCAATAGCGAAACCTATAAAGATGCGTATAGCCGTATTA 
               
               
                   
                   
                 ACGCCATTGTGATCGAAGGCGAGCAAGAAGCATACCAAAACTACCTGGACATGGCGCAACTGCTGCCGGA 
               
               
                   
                   
                 GGACGAGGCTGAGCTGATTCGTTTGAGCAAGATGGAGAACCGTCACAAAAAGGGTTTTCAAGCGTGCGGC 
               
               
                   
                   
                 AAGAACCTCAATGTGACTCCGGATATGGATTATGCACAGCAGTTCTTTGCGGAGCTGCACGGCAATTTTC 
               
               
                   
                   
                 AGAAGGCTAAAGCCGAGGGTAAGATTGTTACCTGCCTGCTCATCCAAAGCCTGATCATCGAGGCGTTTGC 
               
               
                   
                   
                 GATTGCAGCCTACAACATTTACATTCCAGTGGCTGATCCGTTTGCACGTAAAATCACCGAGGGTGTCGTC 
               
               
                   
                   
                 AAGGATGAGTATACCCACCTGAATTTCGGCGAAGTTTGGTTGAAGGAACATTTTGAAGCAAGCAAGGCGG 
               
               
                   
                   
                 AGTTGGAGGACGCCAACAAAGAGAACTTACCGCTGGTCTGGCAGATGTTGAACCAGGTCGAAAAGGATGC 
               
               
                   
                   
                 CGAAGTGCTGGGTATGGAGAAAGAGGCTCTGGTGGAGGACTTTATGATTAGCTATGGTGAGGCACTGAGC 
               
               
                   
                   
                 AACATCGGCTTTTCTACGAGAGAAATCATGAAGATGAGCGCGTACGGTCTGCGTGCAGCATAAGAGCTCG 
               
               
                   
                   
                 AGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGA 
               
               
                   
                   
                 CGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTG 
               
               
                   
                   
                 CCCGCCGTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCG 
               
               
                   
                   
                 GCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGT 
               
               
                   
                   
                 GACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCC 
               
               
                   
                   
                 GCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGA 
               
               
                   
                   
                 GTCCCGATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAA 
               
               
                   
                   
                 CGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAA 
               
               
                   
                   
                 TACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATC 
               
               
                   
                   
                 ACCCCGAGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGC 
               
               
                   
                   
                 CGTCACCACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGAC 
               
               
                   
                   
                 CATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACA 
               
               
                   
                   
                 CCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGT 
               
               
                   
                   
                 CAACTTCATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACC 
               
               
                   
                   
                 AGTTACTTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAAC 
               
               
                   
                   
                 TCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCAC 
               
               
                   
                   
                 GCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGA 
               
               
                   
                   
                 CGCGTGATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCC 
               
               
                   
                   
                 TGGGCGCACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGT 
               
               
                   
                   
                 GCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCC 
               
               
                   
                   
                 TACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCA 
               
               
                   
                   
                 CCGCGAGCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCA 
               
               
                   
                   
                 CCTGGTGTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAAC 
               
               
                   
                   
                 CTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTT 
               
               
                   
                   
                 TCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGC 
               
               
                   
                   
                 GCTGGCCGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTC 
               
               
                   
                   
                 ATCGTCGAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCA 
               
               
                   
                   
                 ACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCA 
               
               
                   
                   
                 GTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACG 
               
               
                   
                   
                 ATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGG 
               
               
                   
                   
                 CGCTGACACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCC 
               
               
                   
                   
                 GGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGT 
               
               
                   
                   
                 TTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCG 
               
               
                   
                   
                 ACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTC 
               
               
                   
                   
                 GGGCGCCAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGC 
               
               
                   
                   
                 ACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACA 
               
               
                   
                   
                 CCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACAT 
               
               
                   
                   
                 CGGCGACCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTG 
               
               
                   
                   
                 CATCCGGCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGG 
               
               
                   
                   
                 CCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGC 
               
               
                   
                   
                 CATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGC 
               
               
                   
                   
                 GGATACGCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGT 
               
               
                   
                   
                 GCGGGCTGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAA 
               
               
                   
                   
                 CGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTAC 
               
               
                   
                   
                 ATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCA 
               
               
                   
                   
                 CGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGAT 
               
               
                   
                   
                 CTCCCTGGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGAC 
               
               
                   
                   
                 GACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGC 
               
               
                   
                   
                 CGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCA 
               
               
                   
                   
                 CGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAG 
               
               
                   
                   
                 TACATACGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCAGGAGGTTTTTACATGGACCGTAAAAGC 
               
               
                   
                   
                 AAGCGTCCGGACATGCTGGTTGATTCCTTTGGTCTGGAAAGCACCGTGCAGGACGGTCTGGTTTTCCGTC 
               
               
                   
                   
                 AGTCTTTCTCCATTCGTAGCTATGAGATTGGTACTGATCGTACCGCCTCTATCGAAACCCTGATGAATCA 
               
               
                   
                   
                 CCTGCAAGAAACCTCTCTGAACCATTGTAAGTCTACTGGCATCCTGCTGGACGGTTTCGGTCGTACCCTG 
               
               
                   
                   
                 GAGATGTGCAAACGCGACCTGATTTGGGTAGTGATCAAAATGCAGATCAAAGTTAACCGTTATCCGGCAT 
               
               
                   
                   
                 GGGGTGATACCGTTGAAATCAACACCCGCTTTTCTCGTCTGGGCAAAATCGGTATGGGCCGTGACTGGCT 
               
               
                   
                   
                 GATCTCTGACTGTAACACTGGTGAAATTCTGGTTCGTGCTACTAGCGCATACGCGATGATGAACCAGAAA 
               
               
                   
                   
                 ACCCGTCGCCTGAGCAAGCTGCCGTACGAGGTCCACCAGGAGATTGTTCCGCTGTTTGTAGACAGCCCAG 
               
               
                   
                   
                 TGATTGAGGATTCTGACCTGAAAGTGCATAAATTCAAAGTGAAGACCGGTGACAGCATCCAAAAAGGCCT 
               
               
                   
                   
                 GACCCCAGGTTGGAACGATCTGGACGTTAACCAGCACGTTTCCAACGTGAAGTATATCGGTTGGATTCTG 
               
               
                   
                   
                 GAGAGCATGCCGACCGAGGTCCTGGAAACCCAGGAGCTGTGTTCCCTGGCGCTGGAGTACCGCCGTGAGT 
               
               
                   
                   
                 GCGGCCGTGACAGCGTGCTGGAGTCTGTGACCGCTATGGACCCAAGCAAAGTTGGTGTTCGTAGCCAGTA 
               
               
                   
                   
                 CCAGCACCTGCTGCGTCTGGAAGACGGTACTGCTATCGTGAACGGTGCAACTGAATGGCGTCCTAAAAAC 
               
               
                   
                   
                 GCGGGTGCAAACGGTGCTATCAGCACCGGTAAAACCTCTAACGGTAACTCCGTGAGCTAAAAGCTTGTTG 
               
               
                   
                   
                 CTGCATGCAGGAGGTTTTTACAATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACAT 
               
               
                   
                   
                 TTCGTCGAATTTGATCCGGCGAACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGC 
               
               
                   
                   
                 AGCACGCAGGCCGTAAGCGTAAAACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGA 
               
               
                   
                   
                 GTACGGCTACAAATGCGTGCCGGCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGT 
               
               
                   
                   
                 TCCATCTCCCACTGCGGTACTACCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAG 
               
               
                   
                   
                 AGATATTCTCTGTCCAGACGGCACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCT 
               
               
                   
                   
                 GGCGGACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAG 
               
               
                   
                   
                 GCTTCCGAGATCCAAACCGATGCGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTA 
               
               
                   
                   
                 TCATTCACCGTGAGAATGAGATGTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTG 
               
               
                   
                   
                 CCAGCACGACTGAGAATTCGGTTTTCCGTCCTGTCTTGATTTTCAAGCAAACAATGCCTCCGATTTCTAA 
               
               
                   
                   
                 TCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAAAATATTGTTACAAATTTTTACAGGCTATTAAGC 
               
               
                   
                   
                 CTACCGTCATAAATAATTTGCCATTTACTAGTTTTTAATTAACCAGAACCTTGACCGAACGCAGCGGTGG 
               
               
                   
                   
                 TAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCA 
               
               
                   
                   
                 TCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGC 
               
               
                   
                   
                 AGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACT 
               
               
                   
                   
                 ATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCC 
               
               
                   
                   
                 GCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATG 
               
               
                   
                   
                 AAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCT 
               
               
                   
                   
                 CCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAA 
               
               
                   
                   
                 CTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTG 
               
               
                   
                   
                 ATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACT 
               
               
                   
                   
                 CTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCG 
               
               
                   
                   
                 CCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCG 
               
               
                   
                   
                 GCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGT 
               
               
                   
                   
                 CATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTG 
               
               
                   
                   
                 GAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAATGTCTAACAATTCGTT 
               
               
                   
                   
                 CAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAG 
               
               
                   
                   
                 CCAAACTATCAGGTCAAGTCTGCTTTTATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAG 
               
               
                   
                   
                 ATATATCATGAGGCGCGCCACGAGAAAGAGTTATGACAAATTAAAATTCTGACTCTTAGATTATTTCCAG 
               
               
                   
                   
                 AGAGGCTGATTTTCCCAATCTTTGGGAAAGCCTAAGTTTTTAGATTCTATTTCTGGATACATCTCAAAAG 
               
               
                   
                   
                 TTCTTTTTAAATGCTGTGCAAAATTATGCTCTGGTTTAATTCTGTCTAAGAGATACTGAATACAACATAA 
               
               
                   
                   
                 GCCAGTGAAAATTTTACGGCTGTTTCTTTGATTAATATCCTCCAATACTTCTCTAGAGAGCCATTTTCCT 
               
               
                   
                   
                 TTTAACCTATCAGGCAATTTAGGTGATTCTCCTAGCTGTATATTCCAGAGCCTTGAATGATGAGCGCAAA 
               
               
                   
                   
                 TATTTCTAATATGCGACAAAGACCGTAACCAAGATATAAAAAACTTGTTAGGTAATTGGAAATGAGTATG 
               
               
                   
                   
                 TATTTTTTGTCGTGTCTTAGATGGTAATAAATTTGTGTACATTCTAGATAACTGCCCAAAGGCGATTATC 
               
               
                   
                   
                 TCCAAAGCCATATATGACGGCGGTAGTAGAGGATTTGTGTACTTGTTTCGATAATGCCCGATAAATTCTT 
               
               
                   
                   
                 CTACTTTTTTAGATTGGCAATATTGAGTAATCGAATCGATTAATTCTTGATGCTTCCCAGTGTCATAAAA 
               
               
                   
                   
                 TAAACTTTTATTCAGATACCAATGAGGATCATAATCATGGGAGTAGTGATAAATCATTTGAGTTCTGACT 
               
               
                   
                   
                 GCTACTTCTATCGACTCCGTAGCATTAAAAATAAGCATTCTCAAGGATTTATCAAACTTGTATAGATTTG 
               
               
                   
                   
                 GCCGGCCCGTCAAAAGGGCGACACCCCATAATTAGCCCGGGCGAAAGGCCCAGTCTTTCGACTGAGCCTT 
               
               
                   
                   
                 TCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACG 
               
               
                   
                   
                 GCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAACAAGGG 
               
               
                   
                   
                 GTGTTATGAGCCATATTCAGGTATAAATGGGCTCGCGATAATGTTCAGAATTGGTTAATTGGTTGTAACA 
               
               
                   
                   
                 CTGACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGAT 
               
               
                   
                   
                 AAATGCTTCAATAATATTGAAAAAGGAAGAATATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTT 
               
               
                   
                   
                 TTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT 
               
               
                   
                   
                 CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCC 
               
               
                   
                   
                 CCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGA 
               
               
                   
                   
                 CGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC 
               
               
                   
                   
                 ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATA 
               
               
                   
                   
                 ACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACAT 
               
               
                   
                   
                 GGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT 
               
               
                   
                   
                 GACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAG 
               
               
                   
                   
                 CTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCT 
               
               
                   
                   
                 TCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCG 
               
               
                   
                   
                 CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATG 
               
               
                   
                   
                 AACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGT 
               
               
                   
               
               
                 9 
                 carB 
                 MTSDVHDATDGVTETALDDEQSTRRIAELYATDPEFAAAAPLPAVVDAAHKPGLRLAEILQTLFTGYGDR 
               
               
                   
                 
                   Mycobacterium 
                 
                 PALGYRARELATDEGGRTVTRLLPREDTLTYAQVWSRVQAVAAALRHNFAQPIYPGDAVATIGFASPDYL 
               
               
                   
                 
                   smegmatis 
                 
                 TLDLVCAYLGLVSVPLQHNAPVSRLAPILAEVEPRILTVSAEYLDLAVESVRDVNSVSQLVVEDHHPEVD 
               
               
                   
                   
                 DHRDALARAREQLAGKGIAVTTLDAIADEGAGLPAEPIYTADHDQRLAMILYTSGSTGAPKGAMYTEAMV 
               
               
                   
                   
                 ARLWTMSFITGDPTPVINVNFMPLNHLGGRIPISTAVQNGGTSYFVPESDMSTLFEDLALVRPTELGLVP 
               
               
                   
                   
                 RVADMLYQHHLATVDRLVTQGADELTAEKQAGAELREQVLGGRVITGFVSTAPLAAEMRAFLDITLGAHI 
               
               
                   
                   
                 VDGYGLTETGAVTRDGVIVRPPVIDYKLIDVPELGYFSTDKPYPRGELLVRSQTLTPGYYKRPEVTASVF 
               
               
                   
                   
                 DRDGYYHTGDVMAETAPDHLVYVDRRNNVLKLAQGEFVAVANLEAVESGAALVRQIFVYGNSERSFLLAV 
               
               
                   
                   
                 VVPTPEALEQYDPAALKAALADSLQRTARDAELQSYEVPADFIVETEPFSAANGLLSGVGKLLRPNLKDR 
               
               
                   
                   
                 YGQRLEQMYADIAATQANQLRELRRAAATQPVIDTLTQAAATILGTGSEVASDAHFTDLGGDSLSALTLS 
               
               
                   
                   
                 NLLSDFFGFEVPVGTIVNPATNLAQLAQHIEAQRTAGDRRPSFTTVHGADATEIRASELTLDKFIDAETL 
               
               
                   
                   
                 RAAPGLPKVTTEPRTVLLSGANGWLGRFLTLQWLERLAPVGGTLITIVRGRDDAAARARLTQAYDTDPEL 
               
               
                   
                   
                 SRRFAELADRHLRVVAGDIGDPNLGLTPEIWHRLAAEVDLVVHPAALVNHVLPYRQLFGPNVVGTAEVIK 
               
               
                   
                   
                 LALTERIKPVTYLSTVSVAMGIPDFEEDGDIRTVSPVRPLDGGYANGYGNSKWAGEVLLREAHDLCGLPV 
               
               
                   
                   
                 ATFRSDMILAHPRYRGQVNVPDMFTRLLLSLLITGVAPRSFYIGDGERPRAHYPGLTVDFVAEAVTTLGA 
               
               
                   
                   
                 QQREGYVSYDVMNPHDDGISLDVFVDWLIRAGHPIDRVDDYDDWVRRFETALTALPEKRRAQTVLPLLHA 
               
               
                   
                   
                 FRAPQAPLRGAPEPTEVFHAAVRTAKVGPGDIPHLDEALIDKYIRDLREFGLI 
               
               
                   
               
               
                 10 
                 entD  E.coli   
                 MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKCVP 
               
               
                   
                   
                 AIGELRQPVWPAEVYGSISHCGTTALAVVSRQPIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAF 
               
               
                   
                   
                 SLALTLAFSAKESAFKASEIQTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD 
               
               
                   
               
               
                 11 
                 acrM 
                 MNAKLKKLFQQKVDGKTIIVTGASSGIGLTVSKYLAQAGAHVLLLARTKEKLDEVKAEIEAEGGKATVFP 
               
               
                   
                 
                   Acinetobacter 
                 
                 CDLNDMESIDAVSKEILAAVDHIDILVNNAGRSIRRAVHESVDRFHDFERTMQLNYFGAVRLVLNVLPHM 
               
               
                   
                 sp. M-1 
                 MQRKDGQIINISSIGVLANATRFSAYVASKAALDAFSRCLSAEVHSHKIAITSIYMPLVRTPMIAPTKIY 
               
               
                   
                   
                 KYVPTLSPEEAADLIAYAIVKRPKKIATNLGRLASITYAIAPDINNILMSIGFNLFPSSTASVGEQEKLN 
               
               
                   
                   
                 LIQRAYARLFPGEHW 
               
               
                   
               
               
                 12 
                 fadD  E.coli   
                 MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVNMGEVMTFRKLEERSRAFAAYLQQGLG 
               
               
                   
                   
                 LKKGDRVALMMPNLLQYPVALEGILRAGMIVVNVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVD 
               
               
                   
                   
                 KTAVQHVILTRMGDQLSTAKGTVVNEVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAF 
               
               
                   
                   
                 LQYTGGTTGVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELGGQNL 
               
               
                   
                   
                 LITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQQVVAERWVKLTG 
               
               
                   
                   
                 QYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLVDDDDNEVPPGQPGELCVKGPQVMLGYWQ 
               
               
                   
                   
                 RPDATDEIIKNGWLHTGDIAVMDEEGFLRIVDRKKDMILVSGENVYPNEIEDVVMQHPGVQEVAAVGVPS 
               
               
                   
                   
                 GSSGEAVKIFVVKKDPSLTEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNK 
               
               
                   
                   
                 A 
               
               
                   
               
               
                 13 
                 fatB (C12 
                 MATTSLASAFCSMKAVMLARDGRGMKPRSSDLQLRAGNAPTSLKMINGTKFSYTESLKRLPDWSMLFAVI 
               
               
                   
                 fatty acid) 
                 TTIFSAAEKQWTNLEWKPKPKLPQLLDDHEGLHGLVERRTFAIRSYEVGPDRSTSILAVMNHMQEATLNH 
               
               
                   
                 
                   Umbellularia 
                 
                 AKSVGILGDGFGTTLEMSKRDLMWVVRRTHVAVERYPTWGDTVEVECWIGASGNNGMRRDFLVRDCKTGE 
               
               
                   
                 
                   californica 
                 
                 ILTRCTSLSVLMNTRTRRLSTIPDEVRGEIGPAFIDNVAVKDDEIKKLQKLNDSTADYIQGGLTPRWNDL 
               
               
                   
                   
                 DVNQHVNNLKYVAWVFETVPDSIFESHHISSFTLEYRRECTRDSVLRSLTTVSGGSSEAGLVCDHLLQLE 
               
               
                   
                   
                 GGSEVLRARTEWRPKLTDSFRGISVIPAEPRV 
               
               
                   
               
               
                 14 
                 fatBmat (fatB 
                 MEWKPKPKLPQLLDDHEGLHGLVERRTFAIRSYEVGPDRSTSILAVMNHMQEATLNHAKSVGILGDGFGT 
               
               
                   
                 without 
                 TLEMSKRDLMWVVRRTHVAVERYPTWGDTVEVECWIGASGNNGMRRDFLVRDCKTGEILTRCTSLSVLMN 
               
               
                   
                 leader 
                 TRTRRLSTIPDEVRGEIGPAFIDNVAVKDDEIKKLQKLNDSTADYIQGGLTPRWNDLDVNQHVNNLKYVA 
               
               
                   
                 sequence) 
                 WVFETVPDSIFESHHISSFTLEYRRECTRDSVLRSLTTVSGGSSEAGLVCDHLLQLEGGSEVLRARTEWR 
               
               
                   
                 
                   Umbellularia 
                 
                 PKLTDSFRGISVIPAEPRV 
               
               
                   
                 
                   californica 
                 
                   
               
               
                   
               
               
                 15 
                 fatB2 (C8 C10 
                 MVAAAASSAFFPVPAPGASPKPGKEGNWPSSLSPSFKPKSIPNGGFQVKANDSAHPKANGSAVSLKSGSL 
               
               
                   
                 fatty acid) 
                 NTQEDTSSSPPPRTFLHQLPDWSRLLTAITTVEVKSKRPDMHDRKSKRPDMLVDSFGLESTVQDGLVFRQ 
               
               
                   
                 
                   Cuphea 
                 
                 SFSIRSYEIGTDRTASIETLMNHLQETSLNHCKSTGILLDGFGRTLEMCKRDLIWVVIKMQIKVNRYPAW 
               
               
                   
                 
                   hookeriana 
                 
                 GDTVEINTRFSRLGKIGMGRDWLISDCNTGEILVRATSAYAMMNQKTRRLSKLPYEVHQEIVPLFVDSPV 
               
               
                   
                   
                 IEDSDLKVHKEKVKTGDSIQKGLTPGWNDLDVNQHVSNVKYIGWILESMPTEVLETQELCSLALEYRREC 
               
               
                   
                   
                 GRDSVLESVTAMDPSKVGVRSQYQHLLRLEDGTAIVNGATEWRPKNAGANGAISTGKTSNGNSVS 
               
               
                   
               
               
                 16 
                 fatB2mat (fatB 
                 MDRKSKRPDMLVDSFGLESTVQDGLVFRQSFSIRSYEIGTDRTASIETLMNHLQETSLNHCKSTGILLDG 
               
               
                   
                 2 without 
                 FGRTLEMCKRDLIWVVIKMQIKVNRYPAWGDTVEINTRFSRLGKIGMGRDWLISDCNTGEILVRATSAYA 
               
               
                   
                 leader 
                 MMNQKTRRLSKLPYEVHQEIVPLFVDSPVIEDSDLKVHKEKVKTGDSIQKGLTPGWNDLDVNQHVSNVKY 
               
               
                   
                 sequence) 
                 IGWILESMPTEVLETQELCSLALEYRRECGRDSVLESVTAMDPSKVGVRSQYQHLLRLEDGTAIVNGATE 
               
               
                   
                 
                   Cuphea 
                 
                 WRPKNAGANGAISTGKTSNGNSVS 
               
               
                   
                 
                   hookeriana 
                 
                   
               
               
                   
               
               
                 17 
                 kivd 
                 MYTVGDYLLDRLHELGIEEIFGVPGDYNLQFLDQIISRKDMKWVGNANELNASYMADGYARTKKAAAFLT 
               
               
                   
                 
                   Lactococcus 
                 
                 TFGVGELSAVNGLAGSYAENLPVVEIVGSPTSKVQNEGKEVHHTLADGDFKHFMKMHEPVTAARTLLTAE 
               
               
                   
                 
                   lactis 
                 
                 NATVEIDRVLSALLKERKPVYINLPVDVAAAKAEKPSLPLKKENPTSNTSDQEILNKIQESLKNAKKPIV 
               
               
                   
                   
                 ITGHEIISFGLENTVTQFISKTKLPITTLNFGKSSVDETLPSFLGIYNGKLSEPNLKEFVESADFILMLG 
               
               
                   
                   
                 VKLTDSSTGAFTHHLNENKMISLNIDEGKIFNESIQNFDFESLISSLLDLSGIEYKGKYIDKKQEDFVPS 
               
               
                   
                   
                 NALLSQDRLWQAVENLTQSNETIVAEQGTSFFGASSIFLKPKSHFIGQPLWGSIGYTFPAALGSQIADKE 
               
               
                   
                   
                 SRHLLFIGDGSLQLTVQELGLATREKINPICFIINNDGYTVEREIHGPNQSYNDIPMWNYSKLPESFGAT 
               
               
                   
                   
                 EERVVSKIVRTENEFVSVMKEAQADPNRMYWIELVLAKEDAPKVLKKMGKLFAEQNKS 
               
               
                   
               
               
                 18 
                 carboxylic 
                 ATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGACGAGCAGTCGACCC 
               
               
                   
                 acid 
                 GCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGA 
               
               
                   
                 reductase 
                 CGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACGGTGACCGC 
               
               
                   
                 amplified 
                 CCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGTGACGCGTCTGCTGC 
               
               
                   
                 from 
                 CGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCA 
               
               
                   
                 
                   Mycobacterium 
                 
                 CAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTG 
               
               
                   
                 
                   smegmatis. 
                 
                 ACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACCGGTCAGCC 
               
               
                   
                   
                 GGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAATACCTCGACCTCGC 
               
               
                   
                   
                 AGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATCACCCCGAGGTCGAC 
               
               
                   
                   
                 GACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGG 
               
               
                   
                   
                 ACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGATCAGCGCCT 
               
               
                   
                   
                 CGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACACCGAGGCGATGGTG 
               
               
                   
                   
                 GCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGTCAACTTCATGCCGC 
               
               
                   
                   
                 TCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACC 
               
               
                   
                   
                 GGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCCTGGTTCCG 
               
               
                   
                   
                 CGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCACGCAGGGCGCCGACG 
               
               
                   
                   
                 AACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGG 
               
               
                   
                   
                 ATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATC 
               
               
                   
                   
                 GTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCCACCGGTGA 
               
               
                   
                   
                 TCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCCTACCCGCGTGGCGA 
               
               
                   
                   
                 ACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTC 
               
               
                   
                   
                 GACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGG 
               
               
                   
                   
                 ACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAGGCGGTGTT 
               
               
                   
                   
                 CTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTTTCCTTCTGGCCGTG 
               
               
                   
                   
                 GTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGC 
               
               
                   
                   
                 TGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGA 
               
               
                   
                   
                 GCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCAAAGACCGC 
               
               
                   
                   
                 TACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCAGTTGCGCGAACTGC 
               
               
                   
                   
                 GGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACGATCCTCGGCACCGG 
               
               
                   
                   
                 GAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCG 
               
               
                   
                   
                 AACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCACCAACCTCG 
               
               
                   
                   
                 CCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGTTTCACCACCGTGCA 
               
               
                   
                   
                 CGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCGACGCCGAAACGCTC 
               
               
                   
                   
                 CGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCT 
               
               
                   
                   
                 GGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTCATCACGAT 
               
               
                   
                   
                 CGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACACCGATCCCGAGTTG 
               
               
                   
                   
                 TCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACATCGGCGACCCGAATC 
               
               
                   
                   
                 TGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCT 
               
               
                   
                   
                 GGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGGTGATCAAG 
               
               
                   
                   
                 CTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGCCATGGGGATCCCCG 
               
               
                   
                   
                 ACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGCGGATACGCCAACGG 
               
               
                   
                   
                 CTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTG 
               
               
                   
                   
                 GCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCCAGACATGT 
               
               
                   
                   
                 TCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTACATCGGAGACGGTGA 
               
               
                   
                   
                 GCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCG 
               
               
                   
                   
                 CAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGT 
               
               
                   
                   
                 TCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGGGTGCGTCG 
               
               
                   
                   
                 GTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGCCGCTGCTGCACGCG 
               
               
                   
                   
                 TTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCA 
               
               
                   
                   
                 CCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCT 
               
               
                   
                   
                 GCGTGAGTTCGGTCTGATCTGA 
               
               
                   
               
               
                 19 
                 codon- 
                 ATGCAGCAACTGACCGATCAAAGCAAAGAACTGGACTTCAAGAGCGAGACGTACAAAGACGCCTATAGCC 
               
               
                   
                 optimized 
                 GCATTAACGCGATCGTCATTGAAGGCGAACAAGAGGCGCATGAAAACTACATCACCCTGGCGCAGCTGCT 
               
               
                   
                 
                   Nostoc 
                 
                 GCCTGAGAGCCACGACGAACTGATTCGCCTGAGCAAAATGGAGAGCCGTCACAAGAAAGGTTTTGAGGCG 
               
               
                   
                 
                   punctiforme 
                 
                 TGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCAATTTGCGAAGGAGTTCTTTAGCGGTCTGCACCAGA 
               
               
                   
                   adm . 
                 ATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTCACTTGTTTGTTGATCCAGAGCCTGATTATTGAATG 
               
               
                   
                   
                 CTTTGCTATTGCGGCGTACAACATTTACATTCCGGTCGCCGATGACTTTGCGCGTAAAATCACGGAAGGT 
               
               
                   
                   
                 GTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGGTGAAGTGTGGTTGAAGGAACATTTTGCGGAATCTA 
               
               
                   
                   
                 AAGCCGAATTGGAACTGGCAAATCGCCAGAACCTGCCGATCGTTTGGAAGATGCTGAACCAAGTGGAAGG 
               
               
                   
                   
                 TGATGCACATACGATGGCGATGGAGAAGGACGCATTGGTTGAGGACTTTATGATTCAGTATGGCGAAGCA 
               
               
                   
                   
                 CTGTCCAATATCGGTTTCAGCACCCGTGATATCATGCGTCTGAGCGCCTATGGCCTGATCGGTGCCTAA 
               
               
                   
               
               
                 20 
                 codon- 
                 ATGGAGTGGAAACCAAAACCGAAACTGCCTCAGCTGCTGGATGACCACTTCGGTCTGCACGGCCTGGTTT 
               
               
                   
                 optimized 
                 TCCGTCGTACCTTCGCTATCCGTTCTTACGAAGTCGGCCCTGATCGCTCCACCTCCATCCTGGCGGTAAT 
               
               
                   
                 
                   Umbellularia 
                 
                 GAACCACATGCAGGAAGCAACTCTGAACCATGCGAAAAGCGTAGGTATCCTGGGCGATGGTTTCGGCACT 
               
               
                   
                 
                   californicia 
                 
                 ACTCTGGAGATGTCCAAACGTGATCTGATGTGGGTTGTTCGCCGTACCCATGTCGCGGTTGAACGCTACC 
               
               
                   
                 fatB m  (without 
                 CGACCTGGGGCGATACGGTTGAAGTGGAATGCTGGATCGGCGCGTCCGGCAACAACGGCATGCGTCGCGA 
               
               
                   
                 leader 
                 TTTCCTGGTTCGCGATTGTAAGACGGGCGAGATTCTGACCCGTTGCACGTCCCTGAGCGTTCTGATGAAT 
               
               
                   
                 sequence). 
                 ACCCGTACCCGTCGTCTGAGCACCATCCCGGACGAAGTTCGCGGTGAAATTGGCCCGGCATTCATCGATA 
               
               
                   
                   
                 ACGTTGCAGTAAAAGACGATGAAATCAAGAAACTGCAGAAACTGAATGACTCTACCGCGGACTACATCCA 
               
               
                   
                   
                 GGGTGGTCTGACCCCGCGCTGGAACGACCTGGACGTGAACCAGCACGTCAACAACCTGAAATACGTAGCT 
               
               
                   
                   
                 TGGGTATTCGAAACGGTCCCGGATTCTATCTTCGAATCTCACCACATCAGCTCCTTCACCCTGGAATACC 
               
               
                   
                   
                 GTCGTGAGTGTACCCGTGACTCCGTTCTGCGCTCTCTGACCACGGTATCCGGCGGTAGCTCTGAAGCCGG 
               
               
                   
                   
                 TCTGGTTTGCGATCACCTGCTGCAGCTGGAAGGCGGCAGCGAGGTTCTGCGTGCTCGTACTGAGTGGCGT 
               
               
                   
                   
                 CCGAAGCTGACTGACTCTTTCCGCGGCATCTCTGTTATCCCGGCAGAGCCTCGTGTGTAA 
               
               
                   
               
               
                 21 
                 codon- 
                 ATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGA 
               
               
                   
                 optimized  
                 ACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAA 
               
               
                   
                   E. coli  entD. 
                 AACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCG 
               
               
                   
                   
                 GCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTA 
               
               
                   
                   
                 CCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGC 
               
               
                   
                   
                 ACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTC 
               
               
                   
                   
                 AGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATG 
               
               
                   
                   
                 CGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGAT 
               
               
                   
                   
                 GTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGA 
               
               
                   
               
               
                 22 
                 plasmid 
                 TAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTT 
               
               
                   
                 pAQ4::P(cpcB)- 
                 GAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA 
               
               
                   
                 adm Npu -ermC. 
                 TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTA 
               
               
                   
                   
                 TCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCC 
               
               
                   
                   
                 AGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCAT 
               
               
                   
                   
                 TCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAG 
               
               
                   
                   
                 TGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA 
               
               
                   
                   
                 CCTGGAACGCTGTTTTTCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATG 
               
               
                   
                   
                 CTTGATGGTCGGAAGTGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTG 
               
               
                   
                   
                 GCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAGCGATAGATTG 
               
               
                   
                   
                 TCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATT 
               
               
                   
                   
                 TAATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATATTCTTCCTTTTTCAATATTATTGAAGCATT 
               
               
                   
                   
                 TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTCA 
               
               
                   
                   
                 GTGTTACAACCAATTAACCAATTCTGAACATTATCGCGAGCCCATTTATACCTGAATATGGCTCATAACA 
               
               
                   
                   
                 CCCCTTGTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACG 
               
               
                   
                   
                 CCGTAGCGCCGATGGTAGTGTGGGGACTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACG 
               
               
                   
                   
                 AAAGGCTCAGTCGAAAGACTGGGCCTTTCGCCCGGGCTAATTAGGGGGTGTCGCCCTTTACACGTACTTA 
               
               
                   
                   
                 GTCGCTGAAGGCCTCACTGGCCCCTGCAGGGATGGTGGAATGCTGGTTATCTGGTGGGGATTAAGTGGTG 
               
               
                   
                   
                 TTTTACTAAAGCTTGAACAACTCAAGAAAGATTATATTCGCAATAACTGCCAATAATCCCAGCATCTTGA 
               
               
                   
                   
                 GAAAATCCAGCAAACCGGGGGCAAAACACCAGCAAGAAGCCAGCAGACTATCACCAAATCCCCAGCGTAC 
               
               
                   
                   
                 AGCTAGAAATAACTGAGCAGTTGTATTCAATTACCTTCTGGTCAAGCCGAGGAAATTTCCCCACACCTTA 
               
               
                   
                   
                 TACACCTCTGGAAGGTTTTTTTGACGAAGCGCAAAATATCCACAATCGGCTGGGGACTTCTTCTGTCAGA 
               
               
                   
                   
                 AAATGGCAGAAATTTTTGAATGTGTTGGCGATCGCCCTCATCAATGATTATTAGAGAACTTTTGTCCCTG 
               
               
                   
                   
                 ATGTTGGGAATACTCTTGATGACAATTGTGATTGCTCAAAGAAGAAAGAAATTTGGAGTAAATCTCTAAA 
               
               
                   
                   
                 AGGGGACTGAAATATTTGTATGGTCAGCATGACCACTGAAATGGAGAGAAGTCTAAGACAGTAGATGTCT 
               
               
                   
                   
                 TAGATATAAGCCTCATTAGAAGCCATGCCATAAAACAGATTTTGTGGATGAAACAACTTGAAATAGTTCA 
               
               
                   
                   
                 GTTGTAGACCATGTTATAAACATTTATTCTTAACACAGTGACACATTAATGACTCATATATCCGTCCAAA 
               
               
                   
                   
                 AAAAACTAAAATGTTTGTAAATTTAGTTTTGCGGCCGCGTCGACTTCGTTATAAAATAAACTTAACAAAT 
               
               
                   
                   
                 CTATACCCACCTGTAGAGAAGAGTCCCTGAATATCAAAATGGTGGGATAAAAAGCTCAAAAAGGAAAGTA 
               
               
                   
                   
                 GGCTGTGGTTCCCTAGGCAACAGTCTTCCCTACCCCACTGGAAACTAAAAAAACGAGAAAAGTTCGCACC 
               
               
                   
                   
                 GAACATCAATTGCATAATTTTAGCCCTAAAACATAAGCTGAACGAAACTGGTTGTCTTCCCTTCCCAATC 
               
               
                   
                   
                 CAGGACAATCTGAGAATCCCCTGCAACATTACTTAACAAAAAAGCAGGAATAAAATTAACAAGATGTAAC 
               
               
                   
                   
                 AGACATAAGTCCCATCACCGTTGTATAAAGTTAACTGTGGGATTGCAAAAGCATTCAAGCCTAGGCGCTG 
               
               
                   
                   
                 AGCTGTTTGAGCATCCCGGTGGCCCTTGTCGCTGCCTCCGTGTTTCTCCCTGGATTTATTTAGGTAATAT 
               
               
                   
                   
                 CTCTCATAAATCCCCGGGTAGTTAACGAAAGTTAATGGAGATCAGTAACAATAACTCTAGGGTCATTACT 
               
               
                   
                   
                 TTGGACTCCCTCAGTTTATCCGGGGGAATTGTGTTTAAGAAAATCCCAACTCATAAAGTCAAGTAGGAGA 
               
               
                   
                   
                 TTAATCATATGCAGCAACTGACCGATCAAAGCAAAGAACTGGACTTCAAGAGCGAGACGTACAAAGACGC 
               
               
                   
                   
                 CTATAGCCGCATTAACGCGATCGTCATTGAAGGCGAACAAGAGGCGCATGAAAACTACATCACCCTGGCG 
               
               
                   
                   
                 CAGCTGCTGCCTGAGAGCCACGACGAACTGATTCGCCTGAGCAAAATGGAGAGCCGTCACAAGAAAGGTT 
               
               
                   
                   
                 TTGAGGCGTGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCAATTTGCGAAGGAGTTCTTTAGCGGTCT 
               
               
                   
                   
                 GCACCAGAATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTCACTTGTTTGTTGATCCAGAGCCTGATT 
               
               
                   
                   
                 ATTGAATGCTTTGCTATTGCGGCGTACAACATTTACATTCCGGTCGCCGATGACTTTGCGCGTAAAATCA 
               
               
                   
                   
                 CGGAAGGTGTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGGTGAAGTGTGGTTGAAGGAACATTTTGC 
               
               
                   
                   
                 GGAATCTAAAGCCGAATTGGAACTGGCAAATCGCCAGAACCTGCCGATCGTTTGGAAGATGCTGAACCAA 
               
               
                   
                   
                 GTGGAAGGTGATGCACATACGATGGCGATGGAGAAGGACGCATTGGTTGAGGACTTTATGATTCAGTATG 
               
               
                   
                   
                 GCGAAGCACTGTCCAATATCGGTTTCAGCACCCGTGATATCATGCGTCTGAGCGCCTATGGCCTGATCGG 
               
               
                   
                   
                 TGCCTAACTCGAGCAATTCGGTTTTCCGTCCTGTCTTGATTTTCAAGCAAACAATGCCTCCGATTTCTAA 
               
               
                   
                   
                 TCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAAAATATTGTTACAAATTTTTACAGGCTATTAAGC 
               
               
                   
                   
                 CTACCGTCATAAATAATTTGCCATTTACTAGTTTTAATTAACGTGCTATAATTATACTAATTTTATAAGG 
               
               
                   
                   
                 AGGAAAAAATATGGGCATTTTTAGTATTTTTGTAATCAGCACAGTTCATTATCAACCAAACAAAAAATAA 
               
               
                   
                   
                 GTGGTTATAATGAATCGTTAATAAGCAAAATTCATATAACCAAATTAAAGAGGGTTATAATGAACGAGAA 
               
               
                   
                   
                 AAATATAAAACACAGTCAAAACTTTATTACTTCAAAACATAATATAGATAAAATAATGACAAATATAAGA 
               
               
                   
                   
                 TTAAATGAACATGATAATATCTTTGAAATCGGCTCAGGAAAAGGCCATTTTACCCTTGAATTAGTAAAGA 
               
               
                   
                   
                 GGTGTAATTTCGTAACTGCCATTGAAATAGACCATAAATTATGCAAAACTACAGAAAATAAACTTGTTGA 
               
               
                   
                   
                 TCACGATAATTTCCAAGTTTTAAACAAGGATATATTGCAGTTTAAATTTCCTAAAAACCAATCCTATAAA 
               
               
                   
                   
                 ATATATGGTAATATACCTTATAACATAAGTACGGATATAATACGCAAAATTGTTTTTGATAGTATAGCTA 
               
               
                   
                   
                 ATGAGATTTATTTAATCGTGGAATACGGGTTTGCTAAAAGATTATTAAATACAAAACGCTCATTGGCATT 
               
               
                   
                   
                 ACTTTTAATGGCAGAAGTTGATATTTCTATATTAAGTATGGTTCCAAGAGAATATTTTCATCCTAAACCT 
               
               
                   
                   
                 AAAGTGAATAGCTCACTTATCAGATTAAGTAGAAAAAAATCAAGAATATCACACAAAGATAAACAAAAGT 
               
               
                   
                   
                 ATAATTATTTCGTTATGAAATGGGTTAACAAAGAATACAAGAAAATATTTACAAAAAATCAATTTAACAA 
               
               
                   
                   
                 TTCCTTAAAACATGCAGGAATTGACGATTTAAACAATATTAGCTTTGAACAATTCTTATCTCTTTTCAAT 
               
               
                   
                   
                 AGCTATAAATTATTTAATAAGTAAGTTAAGGGATGCATAAACTGCATCCCTTAACTTGTTTTTCGTGTGC 
               
               
                   
                   
                 CTATTTTTTGTGGCGCGCCCAGTTTCCTTTACTGGCCCTAAAGTCGCTGTGGCTAGGGTTCCGAAGGGGC 
               
               
                   
                   
                 ATTATTGGCTCGCGGCTTTACAACCTTGATAAGGAGAGAGATGACAGTTTTTTTTCTCTTTTGCTTAGTA 
               
               
                   
                   
                 AAACAGCAAATTTAAGGCATGTTAAAGAGCAGTAGAACGAAATGGTTGAGCCGGCCTCGATACACTCAAT 
               
               
                   
                   
                 TAACTACTAATAGCTTCAATAAATTTTGGGACGATTGAAGCTATTTTTTTGAAAATCAACTCTTAATATC 
               
               
                   
                   
                 TCCTGTCTCAAAAGAGTTAATTGCTAAACAAAAGCCAGTTTCAGCGAAAAATCTAGAGTTTTATAGGTTC 
               
               
                   
                   
                 GTTCTCAGTACAGGACAAAAAGTTTGAAAAGGATAGAGGGAGAGGGTTTGATGGAAATAAGCACAAATCA 
               
               
                   
                   
                 ATCAAGCCCTCATGAATCAGATTAGCGAAATTCGCCGCCAATTGCGACCTCATCTCGGATGGCATGGAGC 
               
               
                   
                   
                 CAGACTGTCATTTATCGCCCTCTTCCTGGTGGCACTGTTCCGAGCAAAAACCGTCAATCTCGCCAAACTC 
               
               
                   
                   
                 GCCACCGTCTGGGGAGGCAATGCAGCAGAAGAGTCTAATTACAAACGCATGCAGCGATTCTTTCAGTCCT 
               
               
                   
                   
                 TTGACGTCAACATGGACAAAATCGCCAGGATGGTAATGAATATCGCGGCTATCCCGCAACCTTGGGTCTT 
               
               
                   
                   
                 AAGCATCGACCGCACCAACGGCCGGCCTACATGGCCCGTCAATCGAAGGGCGACACAAAATTTATTCTAA 
               
               
                   
                   
                 ATGCATAATAAATACTGATAACATCTTATAGTTTGTATTATATTTTGTATTATCGTTGACATGTATAATT 
               
               
                   
                   
                 TTGATATCAAAAACTGATTTTCCCTTTATTATTTTCGAGATTTATTTTCTTAATTCTCTTTAACAAACTA 
               
               
                   
                   
                 GAAATATTGTATATACAAAAAATCATAAATAATAGATGAATAGTTTAATTATAGGTGTTCATCAATCGAA 
               
               
                   
                   
                 AAAGCAACGTATCTTATTTAAAGTGCGTTGCTTTTTTCTCATTTATAAGGTTAAATAATTCTCATATATC 
               
               
                   
                   
                 AAGCAAAGTGACAGGCGCCCTTAAATATTCTGACAAATGCTCTTTCCCTAAACTCCCCCCATAAAAAAAC 
               
               
                   
                   
                 CCGCCGAAGCGGGTTTTTACGTTATTTGCGGATTAACGATTACTCGTTATCAGAACCGCCCAGGGGGCCC 
               
               
                   
                   
                 GAGCTTAAGACTGGCCGTCGTTTTACAACACAGAAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAG 
               
               
                   
                   
                 GGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTCCCTACTCTCGCCTTCCGCTTCCTCGCTCACTGACTCG 
               
               
                   
                   
                 CTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG 
               
               
                   
                   
                 AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC 
               
               
                   
                   
                 CGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAG 
               
               
                   
                   
                 AGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC 
               
               
                   
                   
                 CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCA 
               
               
                   
                   
                 TAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCC 
               
               
                   
                   
                 CCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT 
               
               
                   
                   
                 TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTT 
               
               
                   
                   
                 CTTGAAGTGGTGGGCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCA 
               
               
                   
                   
                 GTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT 
               
               
                   
                   
                 TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG 
               
               
                   
                   
                 GTCTGACGCTCAGTGGAACGACGCGCGCGTAACTCACGTTAAGGGATTTTGGTCATGAGCTTGCGCCGTC 
               
               
                   
                   
                 CCGTCAAGTCAGCGTAATGCTCTGCTTT 
               
               
                   
               
               
                 23 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTA 
               
               
                   
                 pAQ3::P(nir07- 
                 CGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 fatB m -carB- 
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA 
               
               
                   
                 entD-SpecR. 
                 GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                   
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT 
               
               
                   
                   
                 GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG 
               
               
                   
                   
                 GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT 
               
               
                   
                   
                 CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC 
               
               
                   
                   
                 GGAGCCTATGGAAAAACGOCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCG&lt;+GCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAAC 
               
               
                   
                   
                 TGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGT 
               
               
                   
                   
                 GTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC 
               
               
                   
                   
                 G&lt;AAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATAAATGAGAAAAAAGC 
               
               
                   
                   
                 AACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAACACCTATAATTAAACTATTCATCTATTA 
               
               
                   
                   
                 TTTATGATTTTTTGTATATACAATATTTCTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATA 
               
               
                   
                   
                 ATAAAGGGAAAATCAGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCGCTGAACCTGCAGGC 
               
               
                   
                   
                 GAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAACCCGTCGCCATTGACCCAGAACCGCGCA 
               
               
                   
                   
                 AAGAACGGGAAAAAATTGATCTCGATCTGGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAA 
               
               
                   
                   
                 AGTGAAGTTAGCCGATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGACCTCTTCAAGGATG 
               
               
                   
                   
                 AAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTAAATCGTTCCTGACCGGACTCGCGGAAAA 
               
               
                   
                   
                 AGGCTACGGTGACACCCAACTGAAGGCGATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGAT 
               
               
                   
                   
                 GTCCTGACTTGGGTTGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGACCAATACATTCGAGA 
               
               
                   
                   
                 AGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCATCGAAATCAAATACCAAACCGTTAATGAA 
               
               
                   
                   
                 GGTTTAGTGATCTTGGGTCAGGATATCGGTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAG 
               
               
                   
                   
                 ATGTGG&lt;+TAAAAAAGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCAAAAAGTATCAATGA 
               
               
                   
                   
                 TTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGCATGATTTACAAAAAGTTGTAGTTTCTGT 
               
               
                   
                   
                 TACCAATTGCGAATCGAGAACTGCCTAATCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATG 
               
               
                   
                   
                 GAGTGGAAACCAAAACCGAAACTGCCTCAGCTGCTGGATGACCACTTCGGTCTGCACGGCCTGGTTTTCC 
               
               
                   
                   
                 GTCGTACCTTCGCTATCCGTTCTTACGAAGTCGGCCCTGATCGCTCCACCTCCATCCTGGCGGTAATGAA 
               
               
                   
                   
                 CCACATGCAGGAAGCAACTCTGAACCATGCGAAAAGCGTAGGTATCCTGGGCGATGGTTTCGGCACTACT 
               
               
                   
                   
                 CTGGAGATGTCCAAACGTGATCTGATGTGGGTTGTTCGCCGTACCCATGTCGCGGTTGAACGCTACCCGA 
               
               
                   
                   
                 CCTGGGGCGATACGGTTGAAGTGGAATGCTGGATCGGCGCGTCCGGCAACAACGGCATGCGTCGCGATTT 
               
               
                   
                   
                 CCTGGTTCGCGATTGTAAGACGGGCGAGATTCTGACCCGTTGCACGTCCCTGAGCGTTCTGATGAATACC 
               
               
                   
                   
                 CGTACCCGTCGTCTGAGCACCATCCCGGACGAAGTTCGCGGTGAAATTGGCCCGGCATTCATCGATAACG 
               
               
                   
                   
                 TTGCAGTAAAAGACGATGAAATCAAGAAACTGCAGAAACTGAATGACTCTACCGCGGACTACATCCAGGG 
               
               
                   
                   
                 TGGTCTGACCCCGCGCTGGAACGACCTGGACGTGAACCAGCACGTCAACAACCTGAAATACGTAGCTTGG 
               
               
                   
                   
                 GTATTCGAAACGGTCCCGGATTCTATCTTCGAATCTCACCACATCAGCTCCTTCACCCTGGAATACCGTC 
               
               
                   
                   
                 AAGCTGACTGACTCTTTCCGCGGCATCTCTGTTATCCCGGCAGAGCCTCGTGTGTAAGAGCTCGAGGAGG 
               
               
                   
                   
                 TTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGACGAGCA 
               
               
                   
                   
                 GTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCC 
               
               
                   
                   
                 GTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACG 
               
               
                   
                   
                 GTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGTGACGCG 
               
               
                   
                   
                 TCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCC 
               
               
                   
                   
                 CTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCG 
               
               
                   
                   
                 ATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACC 
               
               
                   
                   
                 GGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAATACCTC 
               
               
                   
                   
                 GACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATCACCCCG 
               
               
                   
                   
                 AGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCAC 
               
               
                   
                   
                 CACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGAT 
               
               
                   
                   
                 CAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACACCGAGG 
               
               
                   
                   
                 CGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGTCAACTT 
               
               
                   
                   
                 CATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTAC 
               
               
                   
                   
                 TTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCC 
               
               
                   
                   
                 TGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCACGCAGGG 
               
               
                   
                   
                 CGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTG 
               
               
                   
                   
                 ATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCXTGGGCG 
               
               
                   
                   
                 CACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCC 
               
               
                   
                   
                 ACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCCTACCCG 
               
               
                   
                   
                 CGTGGCGAACTGCTGCTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCACCGCGA 
               
               
                   
                   
                 GCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGT 
               
               
                   
                   
                 GTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAG 
               
               
                   
                   
                 GCGGTGTTCTCCGGCGCGGCGCTGOTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTTTCCTTC 
               
               
                   
                   
                 TGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGC 
               
               
                   
                   
                 CGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTC 
               
               
                   
                   
                 GAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCA 
               
               
                   
                   
                 AAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCAGTTGCG 
               
               
                   
                   
                 CGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACGATCCTC 
               
               
                   
                   
                 GGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGA 
               
               
                   
                   
                 CACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCAC 
               
               
                   
                   
                 CAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGTTTCACC 
               
               
                   
                   
                 ACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCGACGCCG 
               
               
                   
                   
                 AAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGC 
               
               
                   
                   
                 CAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTC 
               
               
                   
                   
                 ATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACACCGATC 
               
               
                   
                   
                 CCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACATCGGCGA 
               
               
                   
                   
                 CCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCG 
               
               
                   
                   
                 GCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGG 
               
               
                   
                   
                 TGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGCCATGGG 
               
               
                   
                   
                 GATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGCGGATAC 
               
               
                   
                   
                 GCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGC 
               
               
                   
                   
                 TGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCC 
               
               
                   
                   
                 AGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTACATCGGA 
               
               
                   
                   
                 GACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCACGACGC 
               
               
                   
                   
                 TCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCT 
               
               
                   
                   
                 GGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGG 
               
               
                   
                   
                 GTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGCCGCTGC 
               
               
                   
                   
                 TGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCACGCCGC 
               
               
                   
                   
                 GGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATA 
               
               
                   
                   
                 CGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCCACAAGGAGGTTTTTACAATGAAAACGACCCACA 
               
               
                   
                   
                 CCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGAACTTTTGTGAACAAGA 
               
               
                   
                   
                 CCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAAAACTGAACATCTGGCC 
               
               
                   
                   
                 GGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCGGCCATTGGTGAACTGC 
               
               
                   
                   
                 GTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTACCGCGTTGGCGGTTGT 
               
               
                   
                   
                 GTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGCACGCGAGCTGACGGAC 
               
               
                   
                   
                 AACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCC 
               
               
                   
                   
                 TGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATGCGGGCTTCCTGGATTA 
               
               
                   
                   
                 TCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGATGTTTGCCGTCCATTGG 
               
               
                   
                   
                 CAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGAGAATTCGGTTTTCCGTCCTGTCTTG 
               
               
                   
                   
                 ATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGT1TTTGTTTATTGCAAAAACAAAAA 
               
               
                   
                   
                 ATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATTTGCCATTTACTAGTTTTTAA 
               
               
                   
                   
                 TTAACCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACT 
               
               
                   
                   
                 GTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTT 
               
               
                   
                   
                 GATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGG 
               
               
                   
                   
                 GAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAAC 
               
               
                   
                   
                 CGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGA 
               
               
                   
                   
                 TTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAA 
               
               
                   
                   
                 ACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACA 
               
               
                   
                   
                 TCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGC 
               
               
                   
                   
                 AGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGC 
               
               
                   
                   
                 GTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGC 
               
               
                   
                   
                 TAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTAC 
               
               
                   
                   
                 GTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCA 
               
               
                   
                   
                 ATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAG 
               
               
                   
                   
                 AAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAA 
               
               
                   
                   
                 GGTAGTCGGCAAATAATGTCTAACAATTCGTTCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAAGC 
               
               
                   
                   
                 GTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTATTATTTTTAA 
               
               
                   
                   
                 GCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAGGCGCGCCACGAGAAAGAGTTATGACA 
               
               
                   
                   
                 AATTAAAATTCTGACTCTTAGATTATTTCCAGAGAGGCTGATTTTCCCAATCTTTGGGAAAGCCTAAGTT 
               
               
                   
                   
                 TTTAGATTCTATTTCTGGATACATCTCAAAAGTTCTTTTTAAATGCTGTGCAAAATTATGCTCTGGTTTA 
               
               
                   
                   
                 ATTCTGTCTAAGAGATACTGAATACAACATAAGCCAGTGAAAATTTTACGGCTGTTTCTTTGATTAATAT 
               
               
                   
                   
                 CCTCCAATACTTCTCTAGAGAGCCATTTTCCTTTTAACCTATCAGGCAATTTAGGTGATTCTCCTAGCTG 
               
               
                   
                   
                 TATATTCCAGAGCCTTGAATGATGAGCGCAAATATTTCTAATATGCGACAAAGACCGTAACCAAGATATA 
               
               
                   
                   
                 AAAAACTTGTTAGGTAATTGGAAATGAGTATGTATTTTTTGTCGTGTCTTAGATGGTAATAAATTTGTGT 
               
               
                   
                   
                 ACATTCTAGATAACTGCCCAAAGGCGATTATCTCCAAAGCCATATATGACGGCGGTAGTAGAGGATTTGT 
               
               
                   
                   
                 GTACTTGTTTCGATAATGCCCGATAAATTCTTCTACTTTTTTAGATTGGCAATATTGAGTAATCGAATCG 
               
               
                   
                   
                 ATTAATTCTTGATGCTTCCCAGTGTCATAAAATAAACTTTTATTCAGATACCAATGAGGATCATAATCAT 
               
               
                   
                   
                 GGGAGTAGTGATAAATCATTTGAGTTCTGACTGCTACTTCTATCGACTCCGTAGCATTAAAAATAAGCAT 
               
               
                   
                   
                 TCTCAAGGATTTATCAAACTTGTATAGATTTGGCCGGCCCGTCAAAAGGGCGACACCCCATAATTAGCCC 
               
               
                   
                   
                 GGGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCA 
               
               
                   
                   
                 TGGGGAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGA 
               
               
                   
                   
                 CCACCGCGCTACTGCCGCCAGGCAAACAAGGGGTGTTATGAGCCATATTCAGGTATAAATGGGCTCGCGA 
               
               
                   
                   
                 TAATGTTCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATTTGTTTATTTTTCTAAATACATTCAAA 
               
               
                   
                   
                 TATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAATATGAGT 
               
               
                   
                   
                 ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAG 
               
               
                   
                   
                 AAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCT 
               
               
                   
                   
                 CAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT 
               
               
                   
                   
                 CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATT 
               
               
                   
                   
                 CTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGA 
               
               
                   
                   
                 ATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGA 
               
               
                   
                   
                 CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG 
               
               
                   
                   
                 AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCG 
               
               
                   
                   
                 CAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT 
               
               
                   
                   
                 AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCG 
               
               
                   
                   
                 GTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTAT 
               
               
                   
                   
                 CTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG 
               
               
                   
                   
                 ATTAAGCATTGGT 
               
               
                   
               
               
                 24 
                 carboxylic 
                 ATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGACGAGCAGTCGACCC 
               
               
                   
                 acid 
                 GCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGA 
               
               
                   
                 reductase 
                 CGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACGGTGACCGC 
               
               
                   
                 amplified 
                 CCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGTGACGCGTCTGCTGC 
               
               
                   
                 from 
                 CGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCA 
               
               
                   
                 
                   Mycobacterium 
                 
                 CAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTG 
               
               
                   
                 
                   smegmatis. 
                 
                 ACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACCGGTCAGCC 
               
               
                   
                   
                 GGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAATACCTCGACCTCGC 
               
               
                   
                   
                 AGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATCACCCCGAGGTCGAC 
               
               
                   
                   
                 GACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGG 
               
               
                   
                   
                 ACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGATCAGCGCCT 
               
               
                   
                   
                 CGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACACCGAGGCGATGGTG 
               
               
                   
                   
                 GCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGTCAACTTCATGCCGC 
               
               
                   
                   
                 TCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACC 
               
               
                   
                   
                 GGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCCTGGTTCCG 
               
               
                   
                   
                 CGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCACGCAGGGCGCCGACG 
               
               
                   
                   
                 AACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGG 
               
               
                   
                   
                 ATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATC 
               
               
                   
                   
                 GTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCCACCGGTGA 
               
               
                   
                   
                 TCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCCTACCCGCGTGGCGA 
               
               
                   
                   
                 ACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTC 
               
               
                   
                   
                 GACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGG 
               
               
                   
                   
                 ACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAGGCGGTGTT 
               
               
                   
                   
                 CTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTTTCCTTCTGGCCGTG 
               
               
                   
                   
                 GTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGC 
               
               
                   
                   
                 TGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGA 
               
               
                   
                   
                 GCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCAAAGACCGC 
               
               
                   
                   
                 TACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCAGTTGCGCGAACTGC 
               
               
                   
                   
                 GGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACGATCCTCGGCACCGG 
               
               
                   
                   
                 GAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCG 
               
               
                   
                   
                 AACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCACCAACCTCG 
               
               
                   
                   
                 CCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGTTTCACCACCGTGCA 
               
               
                   
                   
                 CGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCGACGCCGAAACGCTC 
               
               
                   
                   
                 CGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCT 
               
               
                   
                   
                 GGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTCATCACGAT 
               
               
                   
                   
                 CGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACACCGATCCCGAGTTG 
               
               
                   
                   
                 TCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACATCGGCGACCCGAATC 
               
               
                   
                   
                 TGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCT 
               
               
                   
                   
                 GGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGGTGATCAAG 
               
               
                   
                   
                 CTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGCCATGGGGATCCCCG 
               
               
                   
                   
                 ACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGCGGATACGCCAACGG 
               
               
                   
                   
                 CTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTG 
               
               
                   
                   
                 GCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCCAGACATGT 
               
               
                   
                   
                 TCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTACATCGGAGACGGTGA 
               
               
                   
                   
                 GCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCG 
               
               
                   
                   
                 CAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGT 
               
               
                   
                   
                 TCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGGGTGCGTCG 
               
               
                   
                   
                 GTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGCCGCTGCTGCACGCG 
               
               
                   
                   
                 TTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCA 
               
               
                   
                   
                 CCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCT 
               
               
                   
                   
                 GCGTGAGTTCGGTCTGATCTGA 
               
               
                   
               
               
                 25 
                 codon- 
                 ATGCAGCAACTGACCGATCAAAGCAAAGAACTGGACTTCAAGAGCGAGACGTACAAAGACGCCTATAGCC 
               
               
                   
                 optimized 
                 GCATTAACGCGATCGTCATTGAAGGCGAACAAGAGGCGCATGAAAACTACATCACCCTGGCGCAGCTGCT 
               
               
                   
                 
                   Nostoc 
                 
                 GCCTGAGAGCCACGACGAACTGATTCGCCTGAGCAAAATGGAGAGCCGTCACAAGAAAGGTTTTGAGGCG 
               
               
                   
                 
                   punctiforme 
                 
                 TGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCAATTTGCGAAGGAGTTCTTTAGCGGTCTGCACCAGA 
               
               
                   
                 
                   adm. 
                 
                 ATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTCACTTGTTTGTTGATCCAGAGCCTGATTATTGAATG 
               
               
                   
                   
                 CTTTGCTATTGCGGCGTACAACATTTACATTCCGGTCGCCGATGACTTTGCGCGTAAAATCACGGAAGGT 
               
               
                   
                   
                 GTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGGTGAAGTGTGGTTGAAGGAACATTTTGCGGAATCTA 
               
               
                   
                   
                 AAGCCGAATTGGAACTGGCAAATCGCCAGAACCTGCCGATCGTTTGGAAGATGCTGAACCAAGTGGAAGG 
               
               
                   
                   
                 TGATGCACATACGATGGCGATGGAGAAGGACGCATTGGTTGAGGACTTTATGATTCAGTATGGCGAAGCA 
               
               
                   
                   
                 CTGTCCAATATCGGTTTCAGCACCCGTGATATCATGCGTCTGAGCGCCTATGGCCTGATCGGTGCCTAA 
               
               
                   
               
               
                 26 
                 codon- 
                 ATGGAGTGGAAACCAAAACCGAAACTGCCTCAGCTGCTGGATGACCACTTCGGTCTGCACGGCCTGGTTT 
               
               
                   
                 optimized 
                 TCCGTCGTACCTTCGCTATCCGTTCTTACGAAGTCGGCCCTGATCGCTCCACCTCCATCCTGGCGGTAAT 
               
               
                   
                 
                   Umbellularia 
                 
                 GAACCACATGCAGGAAGCAACTCTGAACCATGCGAAAAGCGTAGGTATCCTGGGCGATGGTTTCGGCACT 
               
               
                   
                 
                   californicia 
                 
                 ACTCTGGAGATGTCCAAACGTGATCTGATGTGGGTTGTTCGCCGTACCCATGTCGCGGTTGAACGCTACC 
               
               
                   
                 fatB m  (without 
                 CGACCTGGGGCGATACGGTTGAAGTGGAATGCTGGATCGGCGCGTCCGGCAACAACGGCATGCGTCGCGA 
               
               
                   
                 leader 
                 TTTCCTGGTTCGCGATTGTAAGACGGGCGAGATTCTGACCCGTTGCACGTCCCTGAGCGTTCTGATGAAT 
               
               
                   
                 sequence). 
                 ACCCGTACCCGTCGTCTGAGCACCATCCCGGACGAAGTTCGCGGTGAAATTGGCCCGGCATTCATCGATA 
               
               
                   
                   
                 ACGTTGCAGTAAAAGACGATGAAATCAAGAAACTGCAGAAACTGAATGACTCTACCGCGGACTACATCCA 
               
               
                   
                   
                 GGGTGGTCTGACCCCGCGCTGGAACGACCTGGACGTGAACCAGCACGTCAACAACCTGAAATACGTAGCT 
               
               
                   
                   
                 TGGGTATTCGAAACGGTCCCGGATTCTATCTTCGAATCTCACCACATCAGCTCCTTCACCCTGGAATACC 
               
               
                   
                   
                 GTCGTGAGTGTACCCGTGACTCCGTTCTGCGCTCTCTGACCACGGTATCCGGCGGTAGCTCTGAAGCCGG 
               
               
                   
                   
                 TCTGGTTTGCGATCACCTGCTGCAGCTGGAAGGCGGCAGCGAGGTTCTGCGTGCTCGTACTGAGTGGCGT 
               
               
                   
                   
                 CCGAAGCTGACTGACTCTTTCCGCGGCATCTCTGTTATCCCGGCAGAGCCTCGTGTGTAA 
               
               
                   
               
               
                 27 
                 codon- 
                 ATGGACCGTAAAAGCAAGCGTCCGGACATGCTGGTTGATTCCTTTGGTCTGGAAAGCACCGTGCAGGACG 
               
               
                   
                 optimized 
                 GTCTGGTTTTCCGTCAGTCTTTCTCCATTCGTAGCTATGAGATTGGTACTGATCGTACCGCCTCTATCGA 
               
               
                   
                 
                   Cuphea 
                 
                 AACCCTGATGAATCACCTGCAAGAAACCTCTCTGAACCATTGTAAGTCTACTGGCATCCTGCTGGACGGT 
               
               
                   
                 
                   hookeriana 
                 
                 TTCGGTCGTACCCTGGAGATGTGCAAACGCGACCTGATTTGGGTAGTGATCAAAATGCAGATCAAAGTTA 
               
               
                   
                 fatB2 m   
                 ACCGTTATCCGGCATGGGGTGATACCGTTGAAATCAACACCCGCTTTTCTCGTCTGGGCAAAATCGGTAT 
               
               
                   
                 (without 
                 GGGCCGTGACTGGCTGATCTCTGACTGTAACACTGGTGAAATTCTGGTTCGTGCTACTAGCGCATACGCG 
               
               
                   
                 leader 
                 ATGATGAACCAGAAAACCCGTCGCCTGAGCAAGCTGCCGTACGAGGTCCACCAGGAGATTGTTCCGCTGT 
               
               
                   
                 sequence). 
                 TTGTAGACAGCCCAGTGATTGAGGATTCTGACCTGAAAGTGCATAAATTCAAAGTGAAGACCGGTGACAG 
               
               
                   
                   
                 CATCCAAAAAGGCCTGACCCCAGGTTGGAACGATCTGGACGTTAACCAGCACGTTTCCAACGTGAAGTAT 
               
               
                   
                   
                 ATCGGTTGGATTCTGGAGAGCATGCCGACCGAGGTCCTGGAAACCCAGGAGCTGTGTTCCCTGGCGCTGG 
               
               
                   
                   
                 AGTACCGCCGTGAGTGCGGCCGTGACAGCGTGCTGGAGTCTGTGACCGCTATGGACCCAAGCAAAGTTGG 
               
               
                   
                   
                 TGTTCGTAGCCAGTACCAGCACCTGCTGCGTCTGGAAGACGGTACTGCTATCGTGAACGGTGCAACTGAA 
               
               
                   
                   
                 TGGCGTCCTAAAAACGCGGGTGCAAACGGTGCTATCAGCACCGGTAAAACCTCTAACGGTAACTCCGTGA 
               
               
                   
                   
                 GCTAA 
               
               
                   
               
               
                 28 
                 codon- 
                 ATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGA 
               
               
                   
                 optimized  
                 ACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAA 
               
               
                   
                   E. coli  entD. 
                 AACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCG 
               
               
                   
                   
                 GCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTA 
               
               
                   
                   
                 CCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGC 
               
               
                   
                   
                 ACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTC 
               
               
                   
                   
                 AGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATG 
               
               
                   
                   
                 CGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGAT 
               
               
                   
                   
                 GTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGA 
               
               
                   
               
               
                 29 
                 plasmid 
                 TAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTT 
               
               
                   
                 pAQ4::P(cpcB)- 
                 GAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA 
               
               
                   
                 adm Npu -ermC. 
                 TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTA 
               
               
                   
                   
                 TCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCC 
               
               
                   
                   
                 AGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCAT 
               
               
                   
                   
                 TCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAG 
               
               
                   
                   
                 TGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA 
               
               
                   
                   
                 CCTGGAACGCTGTTTTTCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATG 
               
               
                   
                   
                 CTTGATGGTCGGAAGTGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTG 
               
               
                   
                   
                 GCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAGCGATAGATTG 
               
               
                   
                   
                 TCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATT 
               
               
                   
                   
                 TAATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATATTCTTCCTTTTTCAATATTATTGAAGCATT 
               
               
                   
                   
                 TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTCA 
               
               
                   
                   
                 GTGTTACAACCAATTAACCAATTCTGAACATTATCGCGAGCCCATTTATACCTGAATATGGCTCATAACA 
               
               
                   
                   
                 CCCCTTGTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACG 
               
               
                   
                   
                 CCGTAGCGCCGATGGTAGTGTGGGGACTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACG 
               
               
                   
                   
                 AAAGGCTCAGTCGAAAGACTGGGCCTTTCGCCCGGGCTAATTAGGGGGTGTCGCCCTTTACACGTACTTA 
               
               
                   
                   
                 GTCGCTGAAGGCCTCACTGGCCCCTGCAGGGATGGTGGAATGCTGGTTATCTGGTGGGGATTAAGTGGTG 
               
               
                   
                   
                 TTTTACTAAAGCTTGAACAACTCAAGAAAGATTATATTCGCAATAACTGCCAATAATCCCAGCATCTTGA 
               
               
                   
                   
                 GAAAATCCAGCAAACCGGGGGCAAAACACCAGCAAGAAGCCAGCAGACTATCACCAAATCCCCAGCGTAC 
               
               
                   
                   
                 AGCTAGAAATAACTGAGCAGTTGTATTCAATTACCTTCTGGTCAAGCCGAGGAAATTTCCCCACACCTTA 
               
               
                   
                   
                 TACACCTCTGGAAGGTTTTTTTGACGAAGCGCAAAATATCCACAATCGGCTGGGGACTTCTTCTGTCAGA 
               
               
                   
                   
                 AAATGGCAGAAATTTTTGAATGTGTTGGCGATCGCCCTCATCAATGATTATTAGAGAACTTTTGTCCCTG 
               
               
                   
                   
                 ATGTTGGGAATACTCTTGATGACAATTGTGATTGCTCAAAGAAGAAAGAAATTTGGAGTAAATCTCTAAA 
               
               
                   
                   
                 AGGGGACTGAAATATTTGTATGGTCAGCATGACCACTGAAATGGAGAGAAGTCTAAGACAGTAGATGTCT 
               
               
                   
                   
                 TAGATATAAGCCTCATTAGAAGCCATGCCATAAAACAGATTTTGTGGATGAAACAACTTGAAATAGTTCA 
               
               
                   
                   
                 GTTGTAGACCATGTTATAAACATTTATTCTTAACACAGTGACACATTAATGACTCATATATCCGTCCAAA 
               
               
                   
                   
                 AAAAACTAAAATGTTTGTAAATTTAGTTTTGCGGCCGCGTCGACTTCGTTATAAAATAAACTTAACAAAT 
               
               
                   
                   
                 CTATACCCACCTGTAGAGAAGAGTCCCTGAATATCAAAATGGTGGGATAAAAAGCTCAAAAAGGAAAGTA 
               
               
                   
                   
                 GGCTGTGGTTCCCTAGGCAACAGTCTTCCCTACCCCACTGGAAACTAAAAAAACGAGAAAAGTTCGCACC 
               
               
                   
                   
                 GAACATCAATTGCATAATTTTAGCCCTAAAACATAAGCTGAACGAAACTGGTTGTCTTCCCTTCCCAATC 
               
               
                   
                   
                 CAGGACAATCTGAGAATCCCCTGCAACATTACTTAACAAAAAAGCAGGAATAAAATTAACAAGATGTAAC 
               
               
                   
                   
                 AGACATAAGTCCCATCACCGTTGTATAAAGTTAACTGTGGGATTGCAAAAGCATTCAAGCCTAGGCGCTG 
               
               
                   
                   
                 AGCTGTTTGAGCATCCCGGTGGCCCTTGTCGCTGCCTCCGTGTTTCTCCCTGGATTTATTTAGGTAATAT 
               
               
                   
                   
                 CTCTCATAAATCCCCGGGTAGTTAACGAAAGTTAATGGAGATCAGTAACAATAACTCTAGGGTCATTACT 
               
               
                   
                   
                 TTGGACTCCCTCAGTTTATCCGGGGGAATTGTGTTTAAGAAAATCCCAACTCATAAAGTCAAGTAGGAGA 
               
               
                   
                   
                 TTAATCATATGCAGCAACTGACCGATCAAAGCAAAGAACTGGACTTCAAGAGCGAGACGTACAAAGACGC 
               
               
                   
                   
                 CTATAGCCGCATTAACGCGATCGTCATTGAAGGCGAACAAGAGGCGCATGAAAACTACATCACCCTGGCG 
               
               
                   
                   
                 CAGCTGCTGCCTGAGAGCCACGACGAACTGATTCGCCTGAGCAAAATGGAGAGCCGTCACAAGAAAGGTT 
               
               
                   
                   
                 TTGAGGCGTGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCAATTTGCGAAGGAGTTCTTTAGCGGTCT 
               
               
                   
                   
                 GCACCAGAATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTCACTTGTTTGTTGATCCAGAGCCTGATT 
               
               
                   
                   
                 ATTGAATGCTTTGCTATTGCGGCGTACAACATTTACATTCCGGTCGCCGATGACTTTGCGCGTAAAATCA 
               
               
                   
                   
                 CGGAAGGTGTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGGTGAAGTGTGGTTGAAGGAACATTTTGC 
               
               
                   
                   
                 GGAATCTAAAGCCGAATTGGAACTGGCAAATCGCCAGAACCTGCCGATCGTTTGGAAGATGCTGAACCAA 
               
               
                   
                   
                 GTGGAAGGTGATGCACATACGATGGCGATGGAGAAGGACGCATTGGTTGAGGACTTTATGATTCAGTATG 
               
               
                   
                   
                 GCGAAGCACTGTCCAATATCGGTTTCAGCACCCGTGATATCATGCGTCTGAGCGCCTATGGCCTGATCGG 
               
               
                   
                   
                 TGCCTAACTCGAGCAATTCGGTTTTCCGTCCTGTCTTGATTTTCAAGCAAACAATGCCTCCGATTTCTAA 
               
               
                   
                   
                 TCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAAAATATTGTTACAAATTTTTACAGGCTATTAAGC 
               
               
                   
                   
                 CTACCGTCATAAATAATTTGCCATTTACTAGTTTTAATTAACGTGCTATAATTATACTAATTTTATAAGG 
               
               
                   
                   
                 AGGAAAAAATATGGGCATTTTTAGTATTTTTGTAATCAGCACAGTTCATTATCAACCAAACAAAAAATAA 
               
               
                   
                   
                 GTGGTTATAATGAATCGTTAATAAGCAAAATTCATATAACCAAATTAAAGAGGGTTATAATGAACGAGAA 
               
               
                   
                   
                 AAATATAAAACACAGTCAAAACTTTATTACTTCAAAACATAATATAGATAAAATAATGACAAATATAAGA 
               
               
                   
                   
                 TTAAATGAACATGATAATATCTTTGAAATCGGCTCAGGAAAAGGCCATTTTACCCTTGAATTAGTAAAGA 
               
               
                   
                   
                 GGTGTAATTTCGTAACTGCCATTGAAATAGACCATAAATTATGCAAAACTACAGAAAATAAACTTGTTGA 
               
               
                   
                   
                 TCACGATAATTTCCAAGTTTTAAACAAGGATATATTGCAGTTTAAATTTCCTAAAAACCAATCCTATAAA 
               
               
                   
                   
                 ATATATGGTAATATACCTTATAACATAAGTACGGATATAATACGCAAAATTGTTTTTGATAGTATAGCTA 
               
               
                   
                   
                 ATGAGATTTATTTAATCGTGGAATACGGGTTTGCTAAAAGATTATTAAATACAAAACGCTCATTGGCATT 
               
               
                   
                   
                 ACTTTTAATGGCAGAAGTTGATATTTCTATATTAAGTATGGTTCCAAGAGAATATTTTCATCCTAAACCT 
               
               
                   
                   
                 AAAGTGAATAGCTCACTTATCAGATTAAGTAGAAAAAAATCAAGAATATCACACAAAGATAAACAAAAGT 
               
               
                   
                   
                 ATAATTATTTCGTTATGAAATGGGTTAACAAAGAATACAAGAAAATATTTACAAAAAATCAATTTAACAA 
               
               
                   
                   
                 TTCCTTAAAACATGCAGGAATTGACGATTTAAACAATATTAGCTTTGAACAATTCTTATCTCTTTTCAAT 
               
               
                   
                   
                 AGCTATAAATTATTTAATAAGTAAGTTAAGGGATGCATAAACTGCATCCCTTAACTTGTTTTTCGTGTGC 
               
               
                   
                   
                 CTATTTTTTGTGGCGCGCCCAGTTTCCTTTACTGGCCCTAAAGTCGCTGTGGCTAGGGTTCCGAAGGGGC 
               
               
                   
                   
                 ATTATTGGCTCGCGGCTTTACAACCTTGATAAGGAGAGAGATGACAGTTTTTTTTCTCTTTTGCTTAGTA 
               
               
                   
                   
                 AAACAGCAAATTTAAGGCATGTTAAAGAGCAGTAGAACGAAATGGTTGAGCCGGCCTCGATACACTCAAT 
               
               
                   
                   
                 TAACTACTAATAGCTTCAATAAATTTTGGGACGATTGAAGCTATTTTTTTGAAAATCAACTCTTAATATC 
               
               
                   
                   
                 TCCTGTCTCAAAAGAGTTAATTGCTAAACAAAAGCCAGTTTCAGCGAAAAATCTAGAGTTTTATAGGTTC 
               
               
                   
                   
                 GTTCTCAGTACAGGACAAAAAGTTTGAAAAGGATAGAGGGAGAGGGTTTGATGGAAATAAGCACAAATCA 
               
               
                   
                   
                 ATCAAGCCCTCATGAATCAGATTAGCGAAATTCGCCGCCAATTGCGACCTCATCTCGGATGGCATGGAGC 
               
               
                   
                   
                 CAGACTGTCATTTATCGCCCTCTTCCTGGTGGCACTGTTCCGAGCAAAAACCGTCAATCTCGCCAAACTC 
               
               
                   
                   
                 GCCACCGTCTGGGGAGGCAATGCAGCAGAAGAGTCTAATTACAAACGCATGCAGCGATTCTTTCAGTCCT 
               
               
                   
                   
                 TTGACGTCAACATGGACAAAATCGCCAGGATGGTAATGAATATCGCGGCTATCCCGCAACCTTGGGTCTT 
               
               
                   
                   
                 AAGCATCGACCGCACCAACGGCCGGCCTACATGGCCCGTCAATCGAAGGGCGACACAAAATTTATTCTAA 
               
               
                   
                   
                 ATGCATAATAAATACTGATAACATCTTATAGTTTGTATTATATTTTGTATTATCGTTGACATGTATAATT 
               
               
                   
                   
                 TTGATATCAAAAACTGATTTTCCCTTTATTATTTTCGAGATTTATTTTCTTAATTCTCTTTAACAAACTA 
               
               
                   
                   
                 GAAATATTGTATATACAAAAAATCATAAATAATAGATGAATAGTTTAATTATAGGTGTTCATCAATCGAA 
               
               
                   
                   
                 AAAGCAACGTATCTTATTTAAAGTGCGTTGCTTTTTTCTCATTTATAAGGTTAAATAATTCTCATATATC 
               
               
                   
                   
                 AAGCAAAGTGACAGGCGCCCTTAAATATTCTGACAAATGCTCTTTCCCTAAACTCCCCCCATAAAAAAAC 
               
               
                   
                   
                 CCGCCGAAGCGGGTTTTTACGTTATTTGCGGATTAACGATTACTCGTTATCAGAACCGCCCAGGGGGCCC 
               
               
                   
                   
                 GAGCTTAAGACTGGCCGTCGTTTTACAACACAGAAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAG 
               
               
                   
                   
                 GGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTCCCTACTCTCGCCTTCCGCTTCCTCGCTCACTGACTCG 
               
               
                   
                   
                 CTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG 
               
               
                   
                   
                 AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGC 
               
               
                   
                   
                 CGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAG 
               
               
                   
                   
                 AGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC 
               
               
                   
                   
                 CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCA 
               
               
                   
                   
                 TAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCC 
               
               
                   
                   
                 CCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT 
               
               
                   
                   
                 TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTT 
               
               
                   
                   
                 CTTGAAGTGGTGGGCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCA 
               
               
                   
                   
                 GTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT 
               
               
                   
                   
                 TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG 
               
               
                   
                   
                 GTCTGACGCTCAGTGGAACGACGCGCGCGTAACTCACGTTAAGGGATTTTGGTCATGAGCTTGCGCCGTC 
               
               
                   
                   
                 CCGTCAAGTCAGCGTAATGCTCTGCTTT 
               
               
                   
               
               
                 30 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTA 
               
               
                   
                 pAQ3::P(nir07)- 
                 CGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 fatB m -carB- 
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA 
               
               
                   
                 entD-SpecR. 
                 GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                   
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT 
               
               
                   
                   
                 GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG 
               
               
                   
                   
                 GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTG&lt;+CACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT 
               
               
                   
                   
                 CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC 
               
               
                   
                   
                 GGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GeTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAAC 
               
               
                   
                   
                 TGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGT 
               
               
                   
                   
                 GTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC 
               
               
                   
                   
                 G&lt;+AATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATAAATGAGAAAAAAGC 
               
               
                   
                   
                 AACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAACACCTATAATTAAACTATTCATCTATTA 
               
               
                   
                   
                 TTTATGATTTTTTGTATATACAATATTTCTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATA 
               
               
                   
                   
                 ATAAAGGGAAAATCAGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCGCTGAACCTGCAGGC 
               
               
                   
                   
                 GAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAACCCGTCGCCATTGACCCAGAACCGCGCA 
               
               
                   
                   
                 AAGAACGGGAAAAAATTGATCTCGATCTGGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAA 
               
               
                   
                   
                 AGTGAAGTTAGCCGATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGACCTCTTCAAGGATG 
               
               
                   
                   
                 AAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTAAATCGTTCCTGACCGGACTCGCGGAAAA 
               
               
                   
                   
                 AGGCTACGGTGACACCCAACTGAAGGCGATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGAT 
               
               
                   
                   
                 GTCCTGACTTGGGTTGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGACCAATACATTCGAGA 
               
               
                   
                   
                 AGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCATCGAAATCAAATACCAAACCGTTAATGAA 
               
               
                   
                   
                 GGTTTAGTGATCTTGGGTCAGGATATCGGTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAG 
               
               
                   
                   
                 ATGTGGCATAAAAAAGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCAAAAAGTATCAATGA 
               
               
                   
                   
                 TTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGCATGATTTACAAAAAGTTGTAGTTTCTGT 
               
               
                   
                   
                 TACCAATTGCGAATCGAGAACTGCCTAATCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATG 
               
               
                   
                   
                 GAGTGGAAACCAAAACCGAAACTGCCTCAGCTGCTGGATGACCACTTCGGTCTGCACGGCCTGGTTTTCC 
               
               
                   
                   
                 GTCGTACCTTCGCTATCCGTTCTTACGAAGTCGGCCCTGATCGCTCCACCTCCATCCTGGCGGTAATGAA 
               
               
                   
                   
                 CCACATGCAGGAAGCAACTCTGAACCATGCGAAAAGCGTAGGTATCCTGGGCGATGGTTTCGGCACTACT 
               
               
                   
                   
                 CTGGAGATGTCCAAACGTGATCTGATGTGGGTTGTTCGCCGTACCCATGTCGCGGTTGAACGCTACCCGA 
               
               
                   
                   
                 CCTGGGGCGATACGGTTGAAGTGGAATGCTGGATCGGCGCGTCCGGCAACAACGGCATGCGTCGCGATTT 
               
               
                   
                   
                 CCTGGTTCGCGATTGTAAGACGGGCGAGATTCTGACCCGTTGCACGTCCCTGAGCGTTCTGATGAATACC 
               
               
                   
                   
                 CGTACCCGTCGTCTGAGCA{XATCCCGGACGAAGTTCGCGGTGAAATTGGCCCGGCATTCATCGATAACG 
               
               
                   
                   
                 TTGCAGTAAAAGACGATGAAATCAAGAAACTGCAGAAACTGAATGACTCTACCGCGGACTACATCCAGGG 
               
               
                   
                   
                 TGGTCTGACCCCGCGCTGGAACGACCTGGACGTGAACCAGCACGTCAACAACCTGAAATACGTAGCTTGG 
               
               
                   
                   
                 GTATTCGAAACGGTCCCGGATTCTATCTTCGAATCTCACCACATCAGCTCCTTCACCCTGGAATACCGTC 
               
               
                   
                   
                 GTGAGTGTACCCGTGACTCCGTTCTGCGCTCTCTGACCACGGTATCCGGCGGTAGCTCTGAAGCCGGTCT 
               
               
                   
                   
                 GGTTTGCGATCACCTGCTGCAGCTGGAAGGCGGCAGCGAGGTTCTGCGTGCTCGTACTGAGTGGCGTCCG 
               
               
                   
                   
                 AAGCTGACTGACTCTTTCCGCGGCATCTCTGTTATCCCGGCAGAGCCTCGTGTGTAAGAGCTCGAGGAGG 
               
               
                   
                   
                 TTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGACGAGCA 
               
               
                   
                   
                 GTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCC 
               
               
                   
                   
                 GTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACG 
               
               
                   
                   
                 GTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGTGACGCG 
               
               
                   
                   
                 TCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCC 
               
               
                   
                   
                 CTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCG 
               
               
                   
                   
                 ATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACC 
               
               
                   
                   
                 GGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAATACCTC 
               
               
                   
                   
                 GACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATCACCCCG 
               
               
                   
                   
                 AGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCAC 
               
               
                   
                   
                 CACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGAT 
               
               
                   
                   
                 CAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACACCGAGG 
               
               
                   
                   
                 CGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGTCAACTT 
               
               
                   
                   
                 CATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTAC 
               
               
                   
                   
                 TTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCC 
               
               
                   
                   
                 TGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCACGCAGGG 
               
               
                   
                   
                 CGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTG 
               
               
                   
                   
                 ATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCG 
               
               
                   
                   
                 CACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCC 
               
               
                   
                   
                 ACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCCTACCCG 
               
               
                   
                   
                 CGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCACCGCGA 
               
               
                   
                   
                 GCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGT 
               
               
                   
                   
                 GTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAG 
               
               
                   
                   
                 GCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTTTCCTTC 
               
               
                   
                   
                 TGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGC 
               
               
                   
                   
                 CGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTC 
               
               
                   
                   
                 GAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCA 
               
               
                   
                   
                 AAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCAGTTGCG 
               
               
                   
                   
                 CGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACGATCCTC 
               
               
                   
                   
                 GGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGA 
               
               
                   
                   
                 CACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCAC 
               
               
                   
                   
                 CAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGTTTCACC 
               
               
                   
                   
                 ACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCGACGCCG 
               
               
                   
                   
                 AAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGC 
               
               
                   
                   
                 CAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTC 
               
               
                   
                   
                 ATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACACCGATC 
               
               
                   
                   
                 CCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACATCGGCGA 
               
               
                   
                   
                 CCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCG 
               
               
                   
                   
                 GCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGG 
               
               
                   
                   
                 TGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGCCATGGG 
               
               
                   
                   
                 GATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGCGGATAC 
               
               
                   
                   
                 GCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGC 
               
               
                   
                   
                 TGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCC 
               
               
                   
                   
                 AGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTACATCGGA 
               
               
                   
                   
                 GACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCACGACGC 
               
               
                   
                   
                 TCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCT 
               
               
                   
                   
                 GGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGG 
               
               
                   
                   
                 GTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGCCGCTGC 
               
               
                   
                   
                 TGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCACGCCGC 
               
               
                   
                   
                 GGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATA 
               
               
                   
                   
                 CGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCCACAAGGAGGTTTTTACAATGAAAACGACCCACA 
               
               
                   
                   
                 CCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGAACTTTTGTGAACAAGA 
               
               
                   
                   
                 CCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAAAACTGAACATCTGGCC 
               
               
                   
                   
                 GGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCGGCCATTGGTGAACTGC 
               
               
                   
                   
                 GTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTACCGCGTTGGCGGTTGT 
               
               
                   
                   
                 GTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGCACGCGAGCTGACGGAC 
               
               
                   
                   
                 AACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCC 
               
               
                   
                   
                 TGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATGCGGGCTTCCTGGATTA 
               
               
                   
                   
                 TCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGATGTTTGCCGTCCATTGG 
               
               
                   
                   
                 CAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGAGAATTCGGTTTTCCGTCCTGTCTTG 
               
               
                   
                   
                 ATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAAA 
               
               
                   
                   
                 ATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATTTGCCATTTACTAGTTTTTAA 
               
               
                   
                   
                 TTAACCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACT 
               
               
                   
                   
                 GTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTT 
               
               
                   
                   
                 GATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGG 
               
               
                   
                   
                 GAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAAC 
               
               
                   
                   
                 CGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGA 
               
               
                   
                   
                 TTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAA 
               
               
                   
                   
                 ACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACA 
               
               
                   
                   
                 TCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGC 
               
               
                   
                   
                 AGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGC 
               
               
                   
                   
                 GTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGC 
               
               
                   
                   
                 TAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTAC 
               
               
                   
                   
                 GTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCA 
               
               
                   
                   
                 ATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAG 
               
               
                   
                   
                 AAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAA 
               
               
                   
                   
                 GGTAGTCGGCAAATAATGTCTAACAATTCGTTCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAAGC 
               
               
                   
                   
                 GTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTATTATTTTTAA 
               
               
                   
                   
                 GCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAGGCGCGCCACGAGAAAGAGTTATGACA 
               
               
                   
                   
                 AATTAAAATTCTGACTCTTAGATTATTTCCAGAGAGGCTGATTTTCCCAATCTTTGGGAAAGCCTAAGTT 
               
               
                   
                   
                 TTTAGATTCTATTTCTGGATACATCTCAAAAGTTCTTTTTAAATGCTGTGCAAAATTATGCTCTGGTTTA 
               
               
                   
                   
                 ATTCTGTCTAAGAGATACTGAATACAACATAAGCCAGTGAAAATTTTACGGCTGTTTCTTTGATTAATAT 
               
               
                   
                   
                 CCTCCAATACTTCTCTAGAGAGCCATTTTCCTTTTAACCTATCAGGCAATTTAGGTGATTCTCCTAGCTG 
               
               
                   
                   
                 TATATTCCAGAGCCTTGAATGATGAGCGCAAATATTTCTAATATGCGACAAAGACCGTAACCAAGATATA 
               
               
                   
                   
                 AAAAACTTGTTAGGTAATTGGAAATGAGTATGTATTTTTTGTCGTGTCTTAGATGGTAATAAATTTGTGT 
               
               
                   
                   
                 ACATTCTAGATAACTGCCCAAAGGCGATTATCTCCAAAGCCATATATGACGGCGGTAGTAGAGGATTTGT 
               
               
                   
                   
                 GTACTTGTTTCGATAATGCCCGATAAATTCTTCTACTTTTTTAGATTGGCAATATTGAGTAATCGAATCG 
               
               
                   
                   
                 ATTAATTCTTGATGCTTCCCAGTGTCATAAAATAAACTTTTATTCAGATACCAATGAGGATCATAATCAT 
               
               
                   
                   
                 GGGAGTAGTGATAAATCATTTGAGTTCTGACTGCTACTTCTATCGACTCCGTAGCATTAAAAATAAGCAT 
               
               
                   
                   
                 TCTCAAGGATTTATCAAACTTGTATAGATTTGGCCGGCCCGTCAAAAGGGCGACACCCCATAATTAGCCC 
               
               
                   
                   
                 GGGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCA 
               
               
                   
                   
                 TGGGGAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGA 
               
               
                   
                   
                 CCACCGCGCTACTGCCGCCAGGCAAACAAGGGGTGTTATGAGCCATATTCAGGTATAAATGGGCTCGCGA 
               
               
                   
                   
                 TAATGTTCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATTTGTTTATTTTTCTAAATACATTCAAA 
               
               
                   
                   
                 TATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAATATGAGT 
               
               
                   
                   
                 ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAG 
               
               
                   
                   
                 AAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCT 
               
               
                   
                   
                 CAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT 
               
               
                   
                   
                 CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATT 
               
               
                   
                   
                 CTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGA 
               
               
                   
                   
                 ATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGA 
               
               
                   
                   
                 CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG 
               
               
                   
                   
                 AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCG 
               
               
                   
                   
                 CAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT 
               
               
                   
                   
                 AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCG 
               
               
                   
                   
                 GTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTAT 
               
               
                   
                   
                 CTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG 
               
               
                   
                   
                 ATTAAGCATTGGT 
               
               
                   
               
               
                 31 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTA 
               
               
                   
                 pAQ3::P(nir07)- 
                 CGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 fatB2 m -carB- 
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA 
               
               
                   
                 entD-SpecR. 
                 GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                   
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT 
               
               
                   
                   
                 GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG 
               
               
                   
                   
                 GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT 
               
               
                   
                   
                 CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC 
               
               
                   
                   
                 GGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAAC 
               
               
                   
                   
                 TGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGT 
               
               
                   
                   
                 GTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC 
               
               
                   
                   
                 GCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATAAATGAGAAAAAAGC 
               
               
                   
                   
                 AACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAACACCTATAATTAAACTATTCATCTATTA 
               
               
                   
                   
                 TTTATGATTTTTTGTATATACAATATTTCTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATA 
               
               
                   
                   
                 ATAAAGGGAAAATCAGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCGCTGAACCTGCAGGC 
               
               
                   
                   
                 GAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAACCCGTCGCCATTGACCCAGAACCGCGCA 
               
               
                   
                   
                 AAGAACGGGAAAAAATTGATCTCGATCTGGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAA 
               
               
                   
                   
                 AGTGAAGTTAGCCGATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGACCTCTTCAAGGATG 
               
               
                   
                   
                 AAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTAAATCGTTCCTGACCGGACTCGCGGAAAA 
               
               
                   
                   
                 AGGCTACGGTGACACCCAACTGAAGGCGATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGAT 
               
               
                   
                   
                 GTCCTGACTTGGGTTGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGACCAATACATTCGAGA 
               
               
                   
                   
                 AGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCATCGAAATCAAATACCAAACCGTTAATGAA 
               
               
                   
                   
                 GGTTTAGTGATCTTGGGTCAGGATATCGGTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAG 
               
               
                   
                   
                 ATGTGGCATAAAAAAGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCAAAAAGTATCAATGA 
               
               
                   
                   
                 TTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGCATGATTTACAAAAAGTTGTAGTTTCTGT 
               
               
                   
                   
                 TACCAATTGCGAATCGAGAACTGCCTAATCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATG 
               
               
                   
                   
                 GACCGTAAAAGCAAGCGTCCGGACATGCTGGTTGATTCCTTTGGTCTGGAAAGCACCGTGCAGGACGGTC 
               
               
                   
                   
                 TGGTTTTCCGTCAGTCTTTCTCCATTCGTAGCTATGAGATTGGTACTGATCGTACCGCCTCTATCGAAAC 
               
               
                   
                   
                 CCTGATGAATCACCTGCAAGAAACCTCTCTGAACCATTGTAAGTCTACTGGCATCCTGCTGGACGGTTTC 
               
               
                   
                   
                 GGTCGTACCCTGGAGATGTGCAAACGCGACCTGATTTGGGTAGTGATCAAAATGCAGATCAAAGTTAACC 
               
               
                   
                   
                 GTTATCCGGCATGGGGTGATACCGTTGAAATCAACACCCGCTTTTCTCGTCTGGGCAAAATCGGTATGGG 
               
               
                   
                   
                 CCGTGACTGGCTGATCTCTGACTGTAACACTGGTGAAATTCTGGTTCGTGCTACTAGCGCATACGCGATG 
               
               
                   
                   
                 ATGAACCAGAAAACCCGTCGCCTGAGCAAGCTGCCGTACGAGGTCCACCAGGAGATTGTTCCGCTGTTTG 
               
               
                   
                   
                 TAGACAGCCCAGTGATTGAGGATTCTGACCTGAAAGTGCATAAATTCAAAGTGAAGACCGGTGACAGCAT 
               
               
                   
                   
                 CCAAAAAGGCCTGACCCCAGGTTGGAACGATCTGGACGTTAACCAGCACGTTTCCAACGTGAAGTATATC 
               
               
                   
                   
                 GGTTGGATTCTGGAGAGCATGCCGACCGAGGTCCTGGAAACCCAGGAGCTGTGTTCCCTGGCGCTGGAGT 
               
               
                   
                   
                 ACCGCCGTGAGTGCGGCCGTGACAGCGTGCTGGAGTCTGTGACCGCTATGGACCCAAGCAAAGTTGGTGT 
               
               
                   
                   
                 TCGTAGCCAGTACCAGCACCTGCTGCGTCTGGAAGACGGTACTGCTATCGTGAACGGTGCAACTGAATGG 
               
               
                   
                   
                 CGTCCTAAAAACGCGGGTGCAAACGGTGCTATCAGCACCGGTAAAACCTCTAACGGTAACTCCGTGAGCT 
               
               
                   
                   
                 AAGAGCTCGAGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGC 
               
               
                   
                   
                 ACTCGACGACGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCC 
               
               
                   
                   
                 GCACCGTTGCCCGCCGTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCC 
               
               
                   
                   
                 TGTTCACCGGCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGG 
               
               
                   
                   
                 GCGCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAA 
               
               
                   
                   
                 GCGGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCG 
               
               
                   
                   
                 GTTTCGCGAGTCCCGATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCT 
               
               
                   
                   
                 GCAGCACAACGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTG 
               
               
                   
                   
                 AGCGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGT 
               
               
                   
                   
                 TCGACCATCACCCCGAGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAA 
               
               
                   
                   
                 GGGCATCGCCGTCACCACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTAC 
               
               
                   
                   
                 ACCGCCGACCATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTG 
               
               
                   
                   
                 CGATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGT 
               
               
                   
                   
                 CATCAACGTCAACTTCATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAAC 
               
               
                   
                   
                 GGTGGAACCAGTTACTTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCC 
               
               
                   
                   
                 CGACCGAACTCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCG 
               
               
                   
                   
                 CCTGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTG 
               
               
                   
                   
                 CTCGGCGGACGCGTGATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCG 
               
               
                   
                   
                 ACATCACCCTGGGCGCACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGG 
               
               
                   
                   
                 TGTGATCGTGCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACC 
               
               
                   
                   
                 GACAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCC 
               
               
                   
                   
                 CCGAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGC 
               
               
                   
                   
                 ACCCGACCACCTGGTGTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCG 
               
               
                   
                   
                 GTCGCCAACCTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCG 
               
               
                   
                   
                 AGCGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCT 
               
               
                   
                   
                 CAAGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCG 
               
               
                   
                   
                 GCCGATTTCATCGTCGAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGC 
               
               
                   
                   
                 TGCGGCCCAACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCA 
               
               
                   
                   
                 GGCCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCC 
               
               
                   
                   
                 GCTGCCACGATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATT 
               
               
                   
                   
                 CCCTGTCGGCGCTGACACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCAT 
               
               
                   
                   
                 CGTGAACCCGGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGC 
               
               
                   
                   
                 AGGCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACA 
               
               
                   
                   
                 AGTTCATCGACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGT 
               
               
                   
                   
                 GTTGCTCTCGGGCGCCAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCT 
               
               
                   
                   
                 GTCGGCGGCACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGG 
               
               
                   
                   
                 CCTACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGC 
               
               
                   
                   
                 CGGTGACATCGGCGACCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGAC 
               
               
                   
                   
                 CTGGTGGTGCATCCGGCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCG 
               
               
                   
                   
                 TGGGCACGGCCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGT 
               
               
                   
                   
                 GTCGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCG 
               
               
                   
                   
                 CTCGACGGCGGATACGCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCC 
               
               
                   
                   
                 ACGATCTGTGCGGGCTGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGG 
               
               
                   
                   
                 TCAGGTCAACGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGG 
               
               
                   
                   
                 TCGTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCG 
               
               
                   
                   
                 AGGCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGA 
               
               
                   
                   
                 CGACGGGATCTCCCTGGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGAC 
               
               
                   
                   
                 GACTACGACGACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGA 
               
               
                   
                   
                 CCGTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGA 
               
               
                   
                   
                 GGTGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTG 
               
               
                   
                   
                 ATCGACAAGTACATACGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCCACAAGGAGGTTTTTACAA 
               
               
                   
                   
                 TGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGAA 
               
               
                   
                   
                 CTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAAA 
               
               
                   
                   
                 ACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCGG 
               
               
                   
                   
                 CCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTAC 
               
               
                   
                   
                 CGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGCA 
               
               
                   
                   
                 CGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTCA 
               
               
                   
                   
                 GCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATGC 
               
               
                   
                   
                 GGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGATG 
               
               
                   
                   
                 TTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGAGAATTCGGTT 
               
               
                   
                   
                 TTCCGTCCTGTCTTGATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGTTTTTGTTTA 
               
               
                   
                   
                 TTGCAAAAACAAAAAATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATTTGCCA 
               
               
                   
                   
                 TTTACTAGTTTTTAATTAACCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCA 
               
               
                   
                   
                 TGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGC 
               
               
                   
                   
                 CGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAG 
               
               
                   
                   
                 TTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCG 
               
               
                   
                   
                 AGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCC 
               
               
                   
                   
                 ACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATC 
               
               
                   
                   
                 AACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTG 
               
               
                   
                   
                 TTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCG 
               
               
                   
                   
                 CAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAA 
               
               
                   
                   
                 GCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGG 
               
               
                   
                   
                 ATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCG 
               
               
                   
                   
                 AAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTC 
               
               
                   
                   
                 GCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTT 
               
               
                   
                   
                 ATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAA 
               
               
                   
                   
                 AGGCGAGATCACCAAGGTAGTCGGCAAATAATGTCTAACAATTCGTTCAAGCCGACGCCGCTTCGCGGCG 
               
               
                   
                   
                 CGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGC 
               
               
                   
                   
                 TTTTATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAGGCGCGCCACGA 
               
               
                   
                   
                 GAAAGAGTTATGACAAATTAAAATTCTGACTCTTAGATTATTTCCAGAGAGGCTGATTTTCCCAATCTTT 
               
               
                   
                   
                 GGGAAAGCCTAAGTTTTTAGATTCTATTTCTGGATACATCTCAAAAGTTCTTTTTAAATGCTGTGCAAAA 
               
               
                   
                   
                 TTATGCTCTGGTTTAATTCTGTCTAAGAGATACTGAATACAACATAAGCCAGTGAAAATTTTACGGCTGT 
               
               
                   
                   
                 TTCTTTGATTAATATCCTCCAATACTTCTCTAGAGAGCCATTTTCCTTTTAACCTATCAGGCAATTTAGG 
               
               
                   
                   
                 TGATTCTCCTAGCTGTATATTCCAGAGCCTTGAATGATGAGCGCAAATATTTCTAATATGCGACAAAGAC 
               
               
                   
                   
                 CGTAACCAAGATATAAAAAACTTGTTAGGTAATTGGAAATGAGTATGTATTTTTTGTCGTGTCTTAGATG 
               
               
                   
                   
                 GTAATAAATTTGTGTACATTCTAGATAACTGCCCAAAGGCGATTATCTCCAAAGCCATATATGACGGCGG 
               
               
                   
                   
                 TAGTAGAGGATTTGTGTACTTGTTTCGATAATGCCCGATAAATTCTTCTACTTTTTTAGATTGGCAATAT 
               
               
                   
                   
                 TGAGTAATCGAATCGATTAATTCTTGATGCTTCCCAGTGTCATAAAATAAACTTTTATTCAGATACCAAT 
               
               
                   
                   
                 GAGGATCATAATCATGGGAGTAGTGATAAATCATTTGAGTTCTGACTGCTACTTCTATCGACTCCGTAGC 
               
               
                   
                   
                 ATTAAAAATAAGCATTCTCAAGGATTTATCAAACTTGTATAGATTTGGCCGGCCCGTCAAAAGGGCGACA 
               
               
                   
                   
                 CCCCATAATTAGCCCGGGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAG 
               
               
                   
                   
                 TTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCA 
               
               
                   
                   
                 TGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAACAAGGGGTGTTATGAGCCATATTCAGGTA 
               
               
                   
                   
                 TAAATGGGCTCGCGATAATGTTCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATTTGTTTATTTTT 
               
               
                   
                   
                 CTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA 
               
               
                   
                   
                 AGGAAGAATATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTG 
               
               
                   
                   
                 TTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTA 
               
               
                   
                   
                 CATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG 
               
               
                   
                   
                 AGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTC 
               
               
                   
                   
                 GCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGG 
               
               
                   
                   
                 CATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG 
               
               
                   
                   
                 ACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTG 
               
               
                   
                   
                 ATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGAT 
               
               
                   
                   
                 GGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC 
               
               
                   
                   
                 TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTG 
               
               
                   
                   
                 ATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTC 
               
               
                   
                   
                 CCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG 
               
               
                   
                   
                 ATAGGTGCCTCACTGATTAAGCATTGGT 
               
               
                   
               
               
                 32 
                 carboxylic 
                 GAGCTCGAGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCAC 
               
               
                   
                 acid 
                 TCGACGACGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGC 
               
               
                   
                 reductase 
                 ACCGTTGCCCGCCGTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTG 
               
               
                   
                 amplified 
                 TTCACCGGCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGC 
               
               
                   
                 from 
                 GCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGC 
               
               
                   
                 
                   Mycobacterium 
                 
                 GGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGT 
               
               
                   
                 
                   smegmatis. 
                 
                 TTCGCGAGTCCCGATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGC 
               
               
                   
                   
                 AGCACAACGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAG 
               
               
                   
                   
                 CGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTC 
               
               
                   
                   
                 GACCATCACCCCGAGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGG 
               
               
                   
                   
                 GCATCGCCGTCACCACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACAC 
               
               
                   
                   
                 CGCCGACCATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCG 
               
               
                   
                   
                 ATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCA 
               
               
                   
                   
                 TCAACGTCAACTTCATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGG 
               
               
                   
                   
                 TGGAACCAGTTACTTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCG 
               
               
                   
                   
                 ACCGAACTCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCC 
               
               
                   
                   
                 TGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCT 
               
               
                   
                   
                 CGGCGGACGCGTGATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGAC 
               
               
                   
                   
                 ATCACCCTGGGCGCACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTG 
               
               
                   
                   
                 TGATCGTGCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGA 
               
               
                   
                   
                 CAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCC 
               
               
                   
                   
                 GAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCAC 
               
               
                   
                   
                 CCGACCACCTGGTGTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGT 
               
               
                   
                   
                 CGCCAACCTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAG 
               
               
                   
                   
                 CGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCA 
               
               
                   
                   
                 AGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGC 
               
               
                   
                   
                 CGATTTCATCGTCGAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTG 
               
               
                   
                   
                 CGGCCCAACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGG 
               
               
                   
                   
                 CCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGC 
               
               
                   
                   
                 TGCCACGATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCC 
               
               
                   
                   
                 CTGTCGGCGCTGACACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCG 
               
               
                   
                   
                 TGAACCCGGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAG 
               
               
                   
                   
                 GCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAG 
               
               
                   
                   
                 TTCATCGACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGT 
               
               
                   
                   
                 TGCTCTCGGGCGCCAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGT 
               
               
                   
                   
                 CGGCGGCACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCC 
               
               
                   
                   
                 TACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCG 
               
               
                   
                   
                 GTGACATCGGCGACCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCT 
               
               
                   
                   
                 GGTGGTGCATCCGGCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTG 
               
               
                   
                   
                 GGCACGGCCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGT 
               
               
                   
                   
                 CGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCT 
               
               
                   
                   
                 CGACGGCGGATACGCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCAC 
               
               
                   
                   
                 GATCTGTGCGGGCTGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTC 
               
               
                   
                   
                 AGGTCAACGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTC 
               
               
                   
                   
                 GTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAG 
               
               
                   
                   
                 GCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACG 
               
               
                   
                   
                 ACGGGATCTCCCTGGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGA 
               
               
                   
                   
                 CTACGACGACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACC 
               
               
                   
                   
                 GTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGG 
               
               
                   
                   
                 TGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGAT 
               
               
                   
                   
                 CGACAAGTACATACGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACC 
               
               
                   
               
               
                 33 
                 codon- 
                 CATATGCAAGAACTGGCCCTGAGAAGCGAGCTGGACTTCAATAGCGAAACCTATAAAGATGCGTATAGCC 
               
               
                   
                 optimized 
                 GTATTAACGCCATTGTGATCGAAGGCGAGCAAGAAGCATACCAAAACTACCTGGACATGGCGCAACTGCT 
               
               
                   
                 
                   Cyanothece 
                 
                 GCCGGAGGACGAGGCTGAGCTGATTCGTTTGAGCAAGATGGAGAACCGTCACAAAAAGGGTTTTCAAGCG 
               
               
                   
                 
                   adm. 
                 
                 TGCGGCAAGAACCTCAATGTGACTCCGGATATGGATTATGCACAGCAGTTCTTTGCGGAGCTGCACGGCA 
               
               
                   
                   
                 ATTTTCAGAAGGCTAAAGCCGAGGGTAAGATTGTTACCTGCCTGCTCATCCAAAGCCTGATCATCGAGGC 
               
               
                   
                   
                 GTTTGCGATTGCAGCCTACAACATTTACATTCCAGTGGCTGATCCGTTTGCACGTAAAATCACCGAGGGT 
               
               
                   
                   
                 GTCGTCAAGGATGAGTATACCCACCTGAATTTCGGCGAAGTTTGGTTGAAGGAACATTTTGAAGCAAGCA 
               
               
                   
                   
                 AGGCGGAGTTGGAGGACGCCAACAAAGAGAACTTACCGCTGGTCTGGCAGATGTTGAACCAGGTCGAAAA 
               
               
                   
                   
                 GGATGCCGAAGTGCTGGGTATGGAGAAAGAGGCTCTGGTGGAGGACTTTATGATTAGCTATGGTGAGGCA 
               
               
                   
                   
                 CTGAGCAACATCGGCTTTTCTACGAGAGAAATCATGAAGATGAGCGCGTACGGTCTGCGTGCAGCATAAG 
               
               
                   
                   
                 AGCTC 
               
               
                   
               
               
                 34 
                 codon- 
                 GAGCTCGAGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCAC 
               
               
                   
                 Optimized  E.   
                 TCGACGACGAGCAGTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGC 
               
               
                   
                   coli  tesA m  and 
                 ACCGTTGCCCGCCGTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTG 
               
               
                   
                   E. coli  entD 
                 TTCACCGGCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGC 
               
               
                   
                 genes. 
                 GCACCGTGACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGC 
               
               
                   
                   
                 GGTCGCCGCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGT 
               
               
                   
                   
                 TTCGCGAGTCCCGATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGC 
               
               
                   
                   
                 AGCACAACGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAG 
               
               
                   
                   
                 CGCCGAATACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTC 
               
               
                   
                   
                 GACCATCACCCCGAGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGG 
               
               
                   
                   
                 GCATCGCCGTCACCACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACAC 
               
               
                   
                   
                 CGCCGACCATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCG 
               
               
                   
                   
                 ATGTACACCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCA 
               
               
                   
                   
                 TCAACGTCAACTTCATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGG 
               
               
                   
                   
                 TGGAACCAGTTACTTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCG 
               
               
                   
                   
                 ACCGAACTCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCC 
               
               
                   
                   
                 TGGTCACGCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCT 
               
               
                   
                   
                 CGGCGGACGCGTGATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGAC 
               
               
                   
                   
                 ATCACCCTGGGCGCACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTG 
               
               
                   
                   
                 TGATCGTGCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGA 
               
               
                   
                   
                 CAAGCCCTACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCC 
               
               
                   
                   
                 GAGGTCACCGCGAGCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCAC 
               
               
                   
                   
                 CCGACCACCTGGTGTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGT 
               
               
                   
                   
                 CGCCAACCTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAG 
               
               
                   
                   
                 CGCAGTTTCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCA 
               
               
                   
                   
                 AGGCCGCGCTGGCCGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGC 
               
               
                   
                   
                 CGATTTCATCGTCGAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTG 
               
               
                   
                   
                 CGGCCCAACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGG 
               
               
                   
                   
                 CCAACCAGTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGC 
               
               
                   
                   
                 TGCCACGATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCC 
               
               
                   
                   
                 CTGTCGGCGCTGACACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCG 
               
               
                   
                   
                 TGAACCCGGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAG 
               
               
                   
                   
                 GCCGAGTTTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAG 
               
               
                   
                   
                 TTCATCGACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGT 
               
               
                   
                   
                 TGCTCTCGGGCGCCAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGT 
               
               
                   
                   
                 CGGCGGCACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCC 
               
               
                   
                   
                 TACGACACCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCG 
               
               
                   
                   
                 GTGACATCGGCGACCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCT 
               
               
                   
                   
                 GGTGGTGCATCCGGCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTG 
               
               
                   
                   
                 GGCACGGCCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGT 
               
               
                   
                   
                 CGGTGGCCATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCT 
               
               
                   
                   
                 CGACGGCGGATACGCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCAC 
               
               
                   
                   
                 GATCTGTGCGGGCTGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTC 
               
               
                   
                   
                 AGGTCAACGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTC 
               
               
                   
                   
                 GTTCTACATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAG 
               
               
                   
                   
                 GCGGTCACGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACG 
               
               
                   
                   
                 ACGGGATCTCCCTGGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGA 
               
               
                   
                   
                 CTACGACGACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACC 
               
               
                   
                   
                 GTACTGCCGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGG 
               
               
                   
                   
                 TGTTCCACGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGAT 
               
               
                   
                   
                 CGACAAGTACATACGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCAGGAGGTTTTTACAATGGCTG 
               
               
                   
                   
                 ATACTTTGTTGATTTTGGGTGATTCTCTCTCTGCAGGCTACCGTATGTCCGCGAGCGCGGCATGGCCGGC 
               
               
                   
                   
                 TCTGCTGAACGATAAGTGGCAGAGCAAGACCAGCGTGGTCAATGCGAGCATCAGCGGCGATACCAGCCAG 
               
               
                   
                   
                 CAGGGTCTGGCACGTCTGCCAGCGCTGCTGAAGCAACACCAGCCGCGTTGGGTGCTGGTTGAACTGGGCG 
               
               
                   
                   
                 GCAATGACGGTCTGCGTGGTTTTCAGCCGCAGCAGACCGAACAAACGTTGCGTCAGATTCTGCAGGACGT 
               
               
                   
                   
                 CAAGGCGGCTAACGCGGAACCGCTGCTGATGCAAATTCGCCTGCCGGCGAATTATGGTCGTCGTTACAAC 
               
               
                   
                   
                 GAGGCTTTCAGCGCCATTTATCCTAAACTGGCTAAAGAGTTTGACGTGCCGCTGCTGCCGTTCTTCATGG 
               
               
                   
                   
                 AAGAGGTCTACCTGAAACCGCAATGGATGCAAGACGACGGTATTCATCCGAATCGTGATGCACAACCTTT 
               
               
                   
                   
                 CATCGCGGATTGGATGGCGAAGCAATTGCAACCGCTGGTGAACCATGACTCGTAAAAGCTTGTTGCTGCA 
               
               
                   
                   
                 TGCAGGAGGTTTTTACAATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGT 
               
               
                   
                   
                 CGAATTTGATCCGGCGAACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCAC 
               
               
                   
                   
                 GCAGGCCGTAAGCGTAAAACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACG 
               
               
                   
                   
                 GCTACAAATGCGTGCCGGCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCAT 
               
               
                   
                   
                 CTCCCACTGCGGTACTACCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATA 
               
               
                   
                   
                 TTCTCTGTCCAGACGGCACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGG 
               
               
                   
                   
                 ACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTC 
               
               
                   
                   
                 CGAGATCCAAACCGATGCGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATT 
               
               
                   
                   
                 CACCGTGAGAATGAGATGTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGC 
               
               
                   
                   
                 ACGACTGAGAATTC 
               
               
                   
               
               
                 35 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTA 
               
               
                   
                 pAQ3::P(nir07)- 
                 CGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 adm-carB- 
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA 
               
               
                   
                 tesAm-entD- 
                 GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                 SpecR. 
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT 
               
               
                   
                   
                 GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG 
               
               
                   
                   
                 GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT 
               
               
                   
                   
                 CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC 
               
               
                   
                   
                 GGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAAC 
               
               
                   
                   
                 TGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGT 
               
               
                   
                   
                 GTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC 
               
               
                   
                   
                 GCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATAAATGAGAAAAAAGC 
               
               
                   
                   
                 AACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAACACCTATAATTAAACTATTCATCTATTA 
               
               
                   
                   
                 TTTATGATTTTTTGTATATACAATATTTCTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATA 
               
               
                   
                   
                 ATAAAGGGAAAATCAGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCGCTGAACCTGCAGGC 
               
               
                   
                   
                 GAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAACCCGTCGCCATTGACCCAGAACCGCGCA 
               
               
                   
                   
                 AAGAACGGGAAAAAATTGATCTCGATCTGGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAA 
               
               
                   
                   
                 AGTGAAGTTAGCCGATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGACCTCTTCAAGGATG 
               
               
                   
                   
                 AAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTAAATCGTTCCTGACCGGACTCGCGGAAAA 
               
               
                   
                   
                 AGGCTACGGTGACACCCAACTGAAGGCGATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGAT 
               
               
                   
                   
                 GTCCTGACTTGGGTTGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGACCAATACATTCGAGA 
               
               
                   
                   
                 AGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCATCGAAATCAAATACCAAACCGTTAATGAA 
               
               
                   
                   
                 GGTTTAGTGATCTTGGGTCAGGATATCGGTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAG 
               
               
                   
                   
                 ATGTGGCATAAAAAAGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCAAAAAGTATCAATGA 
               
               
                   
                   
                 TTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGCATGATTTACAAAAAGTTGTAGTTTCTGT 
               
               
                   
                   
                 TACCAATTGCGAATCGAGAACTGCCTAATCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATG 
               
               
                   
                   
                 CAAGAACTGGCCCTGAGAAGCGAGCTGGACTTCAATAGCGAAACCTATAAAGATGCGTATAGCCGTATTA 
               
               
                   
                   
                 ACGCCATTGTGATCGAAGGCGAGCAAGAAGCATACCAAAACTACCTGGACATGGCGCAACTGCTGCCGGA 
               
               
                   
                   
                 GGACGAGGCTGAGCTGATTCGTTTGAGCAAGATGGAGAACCGTCACAAAAAGGGTTTTCAAGCGTGCGGC 
               
               
                   
                   
                 AAGAACCTCAATGTGACTCCGGATATGGATTATGCACAGCAGTTCTTTGCGGAGCTGCACGGCAATTTTC 
               
               
                   
                   
                 AGAAGGCTAAAGCCGAGGGTAAGATTGTTACCTGCCTGCTCATCCAAAGCCTGATCATCGAGGCGTTTGC 
               
               
                   
                   
                 GATTGCAGCCTACAACATTTACATTCCAGTGGCTGATCCGTTTGCACGTAAAATCACCGAGGGTGTCGTC 
               
               
                   
                   
                 AAGGATGAGTATACCCACCTGAATTTCGGCGAAGTTTGGTTGAAGGAACATTTTGAAGCAAGCAAGGCGG 
               
               
                   
                   
                 AGTTGGAGGACGCCAACAAAGAGAACTTACCGCTGGTCTGGCAGATGTTGAACCAGGTCGAAAAGGATGC 
               
               
                   
                   
                 CGAAGTGCTGGGTATGGAGAAAGAGGCTCTGGTGGAGGACTTTATGATTAGCTATGGTGAGGCACTGAGC 
               
               
                   
                   
                 AACATCGGCTTTTCTACGAGAGAAATCATGAAGATGAGCGCGTACGGTCTGCGTGCAGCATAAGAGCTCG 
               
               
                   
                   
                 AGGAGGTTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGA 
               
               
                   
                   
                 CGAG&lt;+GTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTG 
               
               
                   
                   
                 CCCGCCGTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCG 
               
               
                   
                   
                 GCTACGGTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGT 
               
               
                   
                   
                 GACGCGTCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCC 
               
               
                   
                   
                 GCGGCCCTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGA 
               
               
                   
                   
                 GTCCCGATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAA 
               
               
                   
                   
                 CGCACCGGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAA 
               
               
                   
                   
                 TACCTCGACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATC 
               
               
                   
                   
                 ACCCCGAGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGC 
               
               
                   
                   
                 CGTCACCACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGAC 
               
               
                   
                   
                 CATGATCAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACA 
               
               
                   
                   
                 CCGAGGCGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGT 
               
               
                   
                   
                 CAACTTCATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACC 
               
               
                   
                   
                 AGTTACTTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAAC 
               
               
                   
                   
                 TCGGCCTGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCAC 
               
               
                   
                   
                 GCAGGGCGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGA 
               
               
                   
                   
                 CGCGTGATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCC 
               
               
                   
                   
                 TGGGCGCACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGT 
               
               
                   
                   
                 GCGGCCACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCC 
               
               
                   
                   
                 TACCCGCGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCA 
               
               
                   
                   
                 CCGCGAGCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCA 
               
               
                   
                   
                 CCTGGTGTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAAC 
               
               
                   
                   
                 CTGGAGGCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTT 
               
               
                   
                   
                 TCCTTCTGGCCGTGGTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGC 
               
               
                   
                   
                 GCTGGCCGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTC 
               
               
                   
                   
                 ATCGTCGAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCA 
               
               
                   
                   
                 ACCTCAAAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCA 
               
               
                   
                   
                 GTTGCGCGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACG 
               
               
                   
                   
                 ATCCTCGGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGG 
               
               
                   
                   
                 CGCTGACACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCC 
               
               
                   
                   
                 GGCCACCAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGT 
               
               
                   
                   
                 TTCACCACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCG 
               
               
                   
                   
                 ACGCCGAAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTC 
               
               
                   
                   
                 GGGCGCCAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGC 
               
               
                   
                   
                 ACCCTCATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACA 
               
               
                   
                   
                 CCGATCCCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACAT 
               
               
                   
                   
                 CGGCGACC&lt;MAATCTG&lt;WCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTG 
               
               
                   
                   
                 CATCCGGCAGCGCTGGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGG 
               
               
                   
                   
                 CCGAGGTGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGC 
               
               
                   
                   
                 CATGGGGATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGC 
               
               
                   
                   
                 GGATACGCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGT 
               
               
                   
                   
                 GCGGGCTGCCCGTGGCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAA 
               
               
                   
                   
                 CGTGCCAGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTAC 
               
               
                   
                   
                 ATCGGAGACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCA 
               
               
                   
                   
                 CGACGCTCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGAT 
               
               
                   
                   
                 CTCCCTGGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGAC 
               
               
                   
                   
                 GACTGGGTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGC 
               
               
                   
                   
                 CGCTGCTGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCA 
               
               
                   
                   
                 CGCCGCGGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAG 
               
               
                   
                   
                 TACATACGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCAGGAGGTTTTTACAATGGCTGATACTTT 
               
               
                   
                   
                 GTTGATTTTGGGTGATTCTCTCTCTGCAGGCTACCGTATGTCCGCGAGCGCGGCATGGCCGGCTCTGCTG 
               
               
                   
                   
                 AACGATAAGTGGCAGAGCAAGACCAGCGTGGTCAATGCGAGCATCAGCGGCGATACCAGCCAGCAGGGTC 
               
               
                   
                   
                 TGGCACGTCTGCCAGCGCTGCTGAAGCAACACCAGCCGCGTTGGGTGCTGGTTGAACTGGGCGGCAATGA 
               
               
                   
                   
                 CGGTCTGCGTGGTTTTCAGCCGCAGCAGACCGAACAAACGTTGCGTCAGATTCTGCAGGACGTCAAGGCG 
               
               
                   
                   
                 GCTAACGCGGAACCGCTGCTGATGCAAATTCGCCTGCCGGCGAATTATGGTCGTCGTTACAACGAGGCTT 
               
               
                   
                   
                 TCAGCGCCATTTATCCTAAACTGGCTAAAGAGTTTGACGTGCCGCTGCTGCCGTTCTTCATGGAAGAGGT 
               
               
                   
                   
                 CTACCTGAAACCGCAATGGATGCAAGACGACGGTATTCATCCGAATCGTGATGCACAACCTTTCATCGCG 
               
               
                   
                   
                 GATTGGATGGCGAAGCAATTGCAACCGCTGGTGAACCATGACTCGTAAAAGCTTGTTGCTGCATGCAGGA 
               
               
                   
                   
                 GGTTTTTACAATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTT 
               
               
                   
                   
                 GATCCGGCGAACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCC 
               
               
                   
                   
                 GTAAGCGTAAAACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAA 
               
               
                   
                   
                 ATGCGTGCCGGCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCAC 
               
               
                   
                   
                 TGCGGTACTACCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTG 
               
               
                   
                   
                 TCCAGACGGCACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGG 
               
               
                   
                   
                 TCTGGCGTTCAGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATC 
               
               
                   
                   
                 CAAACCGATGCGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTG 
               
               
                   
                   
                 AGAATGAGATGTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTG 
               
               
                   
                   
                 AGAATTCGGTTTTCCGTCCTGTCTTGATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTT 
               
               
                   
                   
                 GTTTTTGTTTATTGCAAAAACAAAAAATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAA 
               
               
                   
                   
                 ATAATTTGCCATTTACTAGTTTTTAATTAACCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGT 
               
               
                   
                   
                 GGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAA 
               
               
                   
                   
                 GCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCC 
               
               
                   
                   
                 CTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGT 
               
               
                   
                   
                 TGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGC 
               
               
                   
                   
                 GGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGC 
               
               
                   
                   
                 GAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGA 
               
               
                   
                   
                 AGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGA 
               
               
                   
                   
                 GAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCT 
               
               
                   
                   
                 TGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGT 
               
               
                   
                   
                 TCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCT 
               
               
                   
                   
                 GGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGC 
               
               
                   
                   
                 CGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGC 
               
               
                   
                   
                 TAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTC 
               
               
                   
                   
                 CACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAATGTCTAACAATTCGTTCAAGCCGACGCC 
               
               
                   
                   
                 GCTTCGCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAG 
               
               
                   
                   
                 GTCAAGTCTGCTTTTATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAG 
               
               
                   
                   
                 GCGCGCCACGAGAAAGAGTTATGACAAATTAAAATTCTGACTCTTAGATTATTTCCAGAGAGGCTGATTT 
               
               
                   
                   
                 TCCCAATCTTTGGGAAAGCCTAAGTTTTTAGATTCTATTTCTGGATACATCTCAAAAGTTCTTTTTAAAT 
               
               
                   
                   
                 GCTGTGCAAAATTATGCTCTGGTTTAATTCTGTCTAAGAGATACTGAATACAACATAAGCCAGTGAAAAT 
               
               
                   
                   
                 TTTACGGCTGTTTCTTTGATTAATATCCTCCAATACTTCTCTAGAGAGCCATTTTCCTTTTAACCTATCA 
               
               
                   
                   
                 GGCAATTTAGGTGATTCTCCTAGCTGTATATTCCAGAGCCTTGAATGATGAGCGCAAATATTTCTAATAT 
               
               
                   
                   
                 GCGACAAAGACCGTAACCAAGATATAAAAAACTTGTTAGGTAATTGGAAATGAGTATGTATTTTTTGTCG 
               
               
                   
                   
                 TGTCTTAGATGGTAATAAATTTGTGTACATTCTAGATAACTGCCCAAAGGCGATTATCTCCAAAGCCATA 
               
               
                   
                   
                 TATGACGGCGGTAGTAGAGGATTTGTGTACTTGTTTCGATAATGCCCGATAAATTCTTCTACTTTTTTAG 
               
               
                   
                   
                 ATTGGCAATATTGAGTAATCGAATCGATTAATTCTTGATGCTTCCCAGTGTCATAAAATAAACTTTTATT 
               
               
                   
                   
                 CAGATACCAATGAGGATCATAATCATGGGAGTAGTGATAAATCATTTGAGTTCTGACTGCTACTTCTATC 
               
               
                   
                   
                 GACTCCGTAGCATTAAAAATAAGCATTCTCAAGGATTTATCAAACTTGTATAGATTTGGCCGGCCCGTCA 
               
               
                   
                   
                 AAAGGGCGACACCCCATAATTAGCCCGGGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTG 
               
               
                   
                   
                 ATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTC 
               
               
                   
                   
                 TGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAACAAGGGGTGTTATGAGCC 
               
               
                   
                   
                 ATATTCAGGTATAAATGGGCTCGCGATAATGTTCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATT 
               
               
                   
                   
                 TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAAT 
               
               
                   
                   
                 AATATTGAAAAAGGAAGAATATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT 
               
               
                   
                   
                 TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA 
               
               
                   
                   
                 CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTT 
               
               
                   
                   
                 TTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGA 
               
               
                   
                   
                 GCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT 
               
               
                   
                   
                 CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA 
               
               
                   
                   
                 ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGT 
               
               
                   
                   
                 AACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG 
               
               
                   
                   
                 CCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAAC 
               
               
                   
                   
                 AATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTG 
               
               
                   
                   
                 GTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGAT 
               
               
                   
                   
                 GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGAC 
               
               
                   
                   
                 AGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGT 
               
               
                   
               
               
                 36 
                 
                   N. 
                 
                 MQQLTDQSKELDFKSETYKDAYSRINAIVIEGEQEAHENYITLAQLLPESHDELIRLSKMESRHKKGFEA 
               
               
                   
                 
                   punctiforme 
                 
                 CGRNLAVTPDLQFAKEFFSGLHQNFQTAAAEGKVVTCLLIQSLIIECFAIAAYNIYIPVADDFARKITEG 
               
               
                   
                 Adm sequence 
                 VVKEEYSHLNEGEVWLKEHFAESKAELELANRQNLPIVWKMLNQVEGDAHTMAMEKDALVEDFMIQYGEA 
               
               
                   
                 (polypeptide) 
                 LSNIGFSTRDIMRLSAYGLIGA 
               
               
                   
               
               
                 37 
                 
                   N. 
                 
                 ATGCAGCAACTGACCGATCAAAGCAAAGAACTGGACTTCAAGAGCGAGACGTACAAAGACGCCTATAGCC 
               
               
                   
                 
                   punctiforme 
                 
                 GCATTAACGCGATCGTCATTGAAGGCGAACAAGAGGCGCATGAAAACTACATCACCCTGGCGCAGCTGCT 
               
               
                   
                 adm sequence 
                 GCCTGAGAGCCACGACGAACTGATTCGCCTGAGCAAAATGGAGAGCCGTCACAAGAAAGGTTTTGAGGCG 
               
               
                   
                 (nucleotide) , 
                 TGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCAATTTGCGAAGGAGTTCTTTAGCGGTCTGCACCAGA 
               
               
                   
                 codon- 
                 ATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTCACTTGTTTGTTGATCCAGAGCCTGATTATTGAATG 
               
               
                   
                 optimized for 
                 CTTTGCTATTGCGGCGTACAACATTTACATTCCGGTCGCCGATGACTTTGCGCGTAAAATCACGGAAGGT 
               
               
                   
                 
                   E. coli 
                 
                 GTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGGTGAAGTGTGGTTGAAGGAACATTTTGCGGAATCTA 
               
               
                   
                   
                 AAGCCGAATTGGAACTGGCAAATCGCCAGAACCTGCCGATCGTTTGGAAGATGCTGAACCAAGTGGAAGG 
               
               
                   
                   
                 TGATGCACATACGATGGCGATGGAGAAGGACGCATTGGTTGAGGACTTTATGATTCAGTATGGCGAAGCA 
               
               
                   
                   
                 CTGTCCAATATCGGTTTCAGCACCCGTGATATCATGCGTCTGAGCGCCTATGGCCTGATCGGTGCCTAA 
               
               
                   
               
               
                 38 
                 
                   N. 
                 
                 MGSSHHHHHHSQDPMQQLTDQSKELDFKSETYKDAYSRINAIVIEGEQEA 
               
               
                   
                 
                   Imnctiforme 
                 
                 HENYITLAQLLPESHDELIRLSKMESRHKKGFEACGRNLAVTPDLQFAKE 
               
               
                   
                 His-Tagged 
                 FFSGLHQNFQTAAAEGKVVTCLLIQSLIIECFAIAAYNIYIPVADDFARK 
               
               
                   
                 Adm sequence 
                 ITEGVVKEEYSHLNFGEVWLKEHFAESKAELELANRQNLPIVWKMLNQVE 
               
               
                   
                 (polypeptide) 
                 GDAHTMAMEKDALVEDFMIQYGEALSNIGFSTRDIMRLSAYGLIGA 
               
               
                   
               
               
                 39 
                 
                   N. 
                 
                 ATGGGCAGCAGCCATCACCATCATCACCACAGCCAGGATCCGATGCAGCAACTGACCGATCAAAGCAAAG 
               
               
                   
                 
                   Imnctiforme 
                 
                 AACTGGACTTCAAGAGCGAGACGTACAAAGACGCCTATAGCCGCATTAACGCGATCGTCATTGAAGGCGA 
               
               
                   
                 adm sequence 
                 ACAAGAGGCGCATGAAAACTACATCACCCTGGCGCAGCTGCTGCCTGAGAGCCACGACGAACTGATTCGC 
               
               
                   
                 (His-Tagged) 
                 CTGAGCAAAATGGAGAGCCGTCACAAGAAAGGTTTTGAGGCGTGTGGCCGCAATCTGGCGGTGACCCCGG 
               
               
                   
                 (nucleotide) 
                 ACCTGCAATTTGCGAAGGAGTTCTTTAGCGGTCTGCACCAGAATTTCCAGACGGCCGCAGCCGAGGGCAA 
               
               
                   
                   
                 AGTCGTCACTTGTTTGTTGATCCAGAGCCTGATTATTGAATGCTTTGCTATTGCGGCGTACAACATTTAC 
               
               
                   
                   
                 ATTCCGGTCGCCGATGACTTTGCGCGTAAAATCACGGAAGGTGTTGTCAAAGAGGAGTATTCCCACCTGA 
               
               
                   
                   
                 ATTTCGGTGAAGTGTGGTTGAAGGAACATTTTGCGGAATCTAAAGCCGAATTGGAACTGGCAAATCGCCA 
               
               
                   
                   
                 GAACCTGCCGATCGTTTGGAAGATGCTGAACCAAGTGGAAGGTGATGCACATACGATGGCGATGGAGAAG 
               
               
                   
                   
                 GACGCATTGGTTGAGGACTTTATGATTCAGTATGGCGAAGCACTGTCCAATATCGGTTTCAGCACCCGTG 
               
               
                   
                   
                 ATATCATGCGTCTGAGCGCCTATGGCCTGATCGGTGCC 
               
               
                   
               
               
                 40 
                 
                   N. 
                 
                 5&#39;-CATCACCACAGCCAGGATCCGATGCAGCAACTGACCGATCAAAGCAAA 
               
               
                   
                 
                   punctiforme 
                 
                 GAACTGGACTTC-3&#39; 
               
               
                   
                 adm Primer 
                   
               
               
                   
                 UN19 
                   
               
               
                   
               
               
                 41 
                 
                   N. 
                 
                 5&#39;-CGGCCCGCCAAGCTTTTAGGCACCGATCAGGCCATAGGCGCTCAGACG 
               
               
                   
                 
                   punctiforme 
                 
                 CATGATATC-3&#39; 
               
               
                   
                 adm Primer 
                   
               
               
                   
                 UN20 
                   
               
               
                   
               
               
                 42 
                 Plasmid pCDF-  
                 GGGGAATTGTGAGCGGATAACAATTCCCCTGTAGAAATAATTTTGTTTAACTTTAATAAGGAGATATACC 
               
               
                   
                 npu (Table 5 
                 ATGGGCAGCAGCCATCACCATCATCACCACAGCCAGGATCCGATGCAGCAACTGACCGATCAAAGCAAAG 
               
               
                   
                 for key) 
                 AACTGGACTTCAAGAGCGAGACGTACAAAGACGCCTATAGCCGCATTAACGCGATCGTCATTGAAGGCGA 
               
               
                   
                   
                 ACAAGAGGCGCATGAAAACTACATCACCCTGGCGCAGCTGCTGCCTGAGAGCCACGACGAACTGATTCGC 
               
               
                   
                   
                 CTGAGCAAAATGGAGAGCCGTCACAAGAAAGGTTTTGAGGCGTGTGGCCGCAATCTGGCGGTGACCCCGG 
               
               
                   
                   
                 ACCTGCAATTTGCGAAGGAGTTCTTTAGCGGTCTGCACCAGAATTTCCAGACGGCCGCAGCCGAGGGCAA 
               
               
                   
                   
                 AGTCGTCACTTGTTTGTTGATCCAGAGCCTGATTATTGAATGCTTTGCTATTGCGGCGTACAACATTTAC 
               
               
                   
                   
                 ATTCCGGTCGCCGATGACTTTGCGCGTAAAATCACGGAAGGTGTTGTCAAAGAGGAGTATTCCCACCTGA 
               
               
                   
                   
                 ATTTCGGTGAAGTGTGGTTGAAGGAACATTTTGCGGAATCTAAAGCCGAATTGGAACTGGCAAATCGCCA 
               
               
                   
                   
                 GAACCTGCCGATCGTTTGGAAGATGCTGAACCAAGTGGAAGGTGATGCACATACGATGGCGATGGAGAAG 
               
               
                   
                   
                 GACGCATTGGTTGAGGACTTTATGATTCAGTATGGCGAAGCACTGTCCAATATCGGTTTCAGCACCCGTG 
               
               
                   
                   
                 ATATCATGCGTCTGAGCGCCTATGGCCTGATCGGTGCCTAAAGCTTGCGGCCGCATAATGCTTAAGTCGA 
               
               
                   
                   
                 ACAGAAAGTAATCGTATTGTACACGGCCGCATAATCGAAATTAATACGACTCACTATAGGGGAATTGTGA 
               
               
                   
                   
                 GCGGATAACAATTCCCCATCTTAGTATATTAGTTAAGTATAAGAAGGAGATATACATATGGCAGATCTCA 
               
               
                   
                   
                 ATTGGATATCGGCCGGCCACGCGATCGCTGACGTCGGTACCCTCGAGTCTGGTAAAGAAACCGCTGCTGC 
               
               
                   
                   
                 GAAATTTGAACGCCAGCACATGGACTCGTCTACTAGCGCAGCTTAATTAACCTAGGCTGCTGCCACCGCT 
               
               
                   
                   
                 GAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAACCTCAGG 
               
               
                   
                   
                 CATTTGAGAAGCACACGGTCACACTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGC 
               
               
                   
                   
                 GGCTATTTAACGACCCTGCCCTGAACCGACGACCGGGTCATCGTGGCCGGATCTTGCGGCCCCTCGGCTT 
               
               
                   
                   
                 GAACGAATTGTTAGACATTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCTT 
               
               
                   
                   
                 CCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTAT 
               
               
                   
                   
                 GACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTG 
               
               
                   
                   
                 CCGGTTACTGCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGG 
               
               
                   
                   
                 GCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAA 
               
               
                   
                   
                 GAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGA 
               
               
                   
                   
                 TCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCA 
               
               
                   
                   
                 GTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCG 
               
               
                   
                   
                 GAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTT 
               
               
                   
                   
                 TCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTG 
               
               
                   
                   
                 CGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGA 
               
               
                   
                   
                 TAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATACTCTTCCTTTTTCAATATTATTGAAGCATT 
               
               
                   
                   
                 TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGCTAGCT 
               
               
                   
                   
                 CACTCGGTCGCTACGCTCCGGGCGTGAGACTGCGGCGGGCGCTGCGGACACATACAAAGTTACCCACAGA 
               
               
                   
                   
                 TTCCGTGGATAAGCAGGGGACTAACATGTGAGGCAAAACAGCAGGGCCGCGCCGGTGGCGTTTTTCCATA 
               
               
                   
                   
                 GGCTCCGCCCTCCTGCCAGAGTTCACATAAACAGACGCTTTTCCGGTGCATCTGTGGGAGCCGTGAGGCT 
               
               
                   
                   
                 CAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCCGGCGGGTCG 
               
               
                   
                   
                 CTCCCTCTTGCGCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGG 
               
               
                   
                   
                 GAAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGCTCCAAGCTG 
               
               
                   
                   
                 GGCTGTAAGCAAGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCA 
               
               
                   
                   
                 ACCCGGAAAAGCACGGTAAAACGCCACTGGCAGCAGCCATTGGTAACTGGGAGTTCGCAGAGGATTTGTT 
               
               
                   
                   
                 TAGCTAAACACGCGGTTGCTCTTGAAGTGTGCGCCAAAGTCCGGCTACACTGGAAGGACAGATTTGGTTG 
               
               
                   
                   
                 CTGTGCTCTGCGAAAGCCAGTTACCACGGTTAAGCAGTTCCCCAACTGACTTAACCTTCGATCAAACCAC 
               
               
                   
                   
                 CTCCCCAGGTGGTTTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCC 
               
               
                   
                   
                 TTTGATCTTTTCTACTGAACCGCTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTC 
               
               
                   
                   
                 AGCCCCATACGATATAAGTTGTAATTCTCATGTTAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGG 
               
               
                   
                   
                 GTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCG 
               
               
                   
                   
                 TTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCG 
               
               
                   
                   
                 CGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT 
               
               
                   
                   
                 GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG 
               
               
                   
                   
                 AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACT 
               
               
                   
                   
                 ACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGAT 
               
               
                   
                   
                 CGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGA 
               
               
                   
                   
                 CATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAG 
               
               
                   
                   
                 CCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCA 
               
               
                   
                   
                 ATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGT 
               
               
                   
                   
                 CTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGG 
               
               
                   
                   
                 TCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTAC 
               
               
                   
                   
                 AGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTT 
               
               
                   
                   
                 AATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGAC 
               
               
                   
                   
                 TGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTT 
               
               
                   
                   
                 TTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACC 
               
               
                   
                   
                 GGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGG 
               
               
                   
                   
                 CGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTA 
               
               
                   
                   
                 TGCGACTCCTGCATTAGGAAATTAATACGACTCACTATA 
               
               
                   
               
               
                 43 
                 ketoacid 
                 MYTVGDYLLDRLHELGIEEIFGVPGDYNLQFLDQIISRKDMKWVGNANELNASYMADGYARTKKAAAFLT 
               
               
                   
                 decarboxylase 
                 TFGVGELSAVNGLAGSYAENLPVVEIVGSPTSKVQNEGKEVHHTLADGDFKHFMKMHEPVTAARTLLTAE 
               
               
                   
                 KivD 
                 NATVEIDRVLSALLKERKPVYINLPVDVAAAKAEKPSLPLKKENPTSNTSDQEILNKIQESLKNAKKPIV 
               
               
                   
                 (ADA65057) 
                 ITGHEIISFGLENTVTQFISKTKLPITTLNFGKSSVDETLPSFLGIYNGKLSEPNLKEFVESADFILMLG 
               
               
                   
                 from 
                 VKLTDSSTGAFTHHLNENKMISLNIDEGKIFNESIQNFDFESLISSLLDLSGIEYKGKYIDKKQEDFVPS 
               
               
                   
                 
                   Lactococcus 
                 
                 NALLSQDRLWQAVENLTQSNETIVAEQGTSFFGASSIFLKPKSHFIGQPLWGSIGYTFPAALGSQIADKE 
               
               
                   
                   lactis  subsp. 
                 SRHLLFIGDGSLQLTVQELGLAIREKINPICFIINNDGYTVEREIHGPNQSYNDIPMWNYSKLPESFGAT 
               
               
                   
                   lactis  KF147 
                 EERVVSKIVRTENEFVSVMKEAQADPNRMYWIELVLAKEDAPKVLKKMGKLFAEQNKS 
               
               
                   
                 (polypeptide) 
                   
               
               
                   
               
               
                 44 
                 ketoacid 
                 MAPVTIEKEVNQEERHLVSNRSATIPFGEYIFKRLLSIDTKSVFGVPGDFNLSLLEYLYSPSVESAGLRW 
               
               
                   
                 decarboxylase 
                 VGTCNELNAAYAADGYSRYSNKIGCLITTYGVGELSALNGIAGSFAENVKVLHIVGVAKSIDSRSSNFSD 
               
               
                   
                 ARO10 
                 RNLHHLVPQLHDSNFKGPNHKVYHDMVKDRVACSVAYLEDIETACDQVDNVIRDIYKYSKPGYIFVPADF 
               
               
                   
                 (NP_010668) 
                 ADMSVTCDNLVNVPRISQQDCIVYPSENQLSDIINKITSWIYSSKTPAILGDVLTDRYGVSNFLNKLICK 
               
               
                   
                 from 
                 TGIWNESTVMGKSVIDESNPTYMGQYNGKEGLKQVYEHFELCDLVLHFGVDINEINNGHYTFTYKPNAKI 
               
               
                   
                 
                   Saccharomyces 
                 
                 IQFHPNYIRLVDTRQGNEQMFKGINFAPILKELYKRIDVSKLSLQYDSNVTQYTNETMRLEDPTNGQSSI 
               
               
                   
                 
                   cerevisiae 
                 
                 ITQVHLQKTMPKFLNPGDVVVCETGSFQFSVRDFAFPSQLKYISQGFFLSIGMALPAALGVGIAMQDHSN 
               
               
                   
                 S288c 
                 AHINGGNVKEDYKPRLILFEGDGAAQMTIQELSTILKCNIPLEVIIWNNNGYTIERAIMGPTRSYNDVMS 
               
               
                   
                 (polypeptide) 
                 WKWTKLFEAFGDFDGKYTNSTLIQCPSKLALKLEELKNSNKRSGIELLEVKLGELDFPEQLKCMVEAAAL 
               
               
                   
                   
                 KRNKK 
               
               
                   
               
               
                 45 
                 pdc( Z.   
                 ATGAGTTATACTGTCGGTACCTATTTAGCGGAGCGGCTTGTCCAGATTGGTCTCAAGCATCACTTCGCAG 
               
               
                   
                   mobilis ) 
                 TCGCGGGCGACTACAACCTCGTCCTTCTTGACAACCTGCTTTTGAACAAAAACATGGAGCAGGTTTATTG 
               
               
                   
                 nucleotide 
                 CTGTAACGAACTGAACTGCGGTTTCAGTGCAGAAGGTTATGCTCGTGCCAAAGGCGCAGCAGCAGCCGTC 
               
               
                   
                 sequence 
                 GTTACCTACAGCGTCGGTGCGCTTTCCGCATTTGATGCTATCGGTGGCGCCTATGCAGAAAACCTTCCGG 
               
               
                   
                   
                 TTATCCTGATCTCCGGTGCTCCGAACAACAATGATCACGCTGCTGGTCACGTGTTGCATCACGCTCTTGG 
               
               
                   
                   
                 CAAAACCGACTATCACTATCAGTTGGAAATGGCCAAGAACATCACGGCCGCAGCTGAAGCGATTTACACC 
               
               
                   
                   
                 CCAGAAGAAGCTCCGGCTAAAATCGATCACGTGATTAAAACTGCTCTTCGTGAGAAGAAGCCGGTTTATC 
               
               
                   
                   
                 TCGAAATCGCTTGCAACATTGCTTCCATGCCCTGCGCCGCTCCTGGACCGGCAAGCGCATTGTTCAATGA 
               
               
                   
                   
                 CGAAGCCAGCGACGAAGCTTCTTTGAATGCAGCGGTTGAAGAAACCCTGAAATTCATCGCCAACCGCGAC 
               
               
                   
                   
                 AAAGTTGCCGTCCTCGTCGGCAGCAAGCTGCGCGCAGCTGGTGCTGAAGAAGCTGCTGTCAAATTTGCTG 
               
               
                   
                   
                 ATGCTCTCGGTGGCGCAGTTGCTACCATGGCTGCTGCAAAAAGCTTCTTCCCAGAAGAAAACCCGCATTA 
               
               
                   
                   
                 CATCGGTACCTCATGGGGTGAAGTCAGCTATCCGGGCGTTGAAAAGACGATGAAAGAAGCCGATGCGGTT 
               
               
                   
                   
                 ATCGCTCTGGCTCCTGTCTTCAACGACTACTCCACCACTGGTTGGACGGATATTCCTGATCCTAAGAAAC 
               
               
                   
                   
                 TGGTTCTCGCTGAACCGCGTTCTGTCGTCGTTAACGGCGTTCGCTTCCCCAGCGTTCATCTGAAAGACTA 
               
               
                   
                   
                 TCTGACCCGTTTGGCTCAGAAAGTTTCCAAGAAAACCGGTGCTTTGGACTTCTTCAAATCCCTCAATGCA 
               
               
                   
                   
                 GGTGAACTGAAGAAAGCCGCTCCGGCTGATCCGAGTGCTCCGTTGGTCAACGCAGAAATCGCCCGTCAGG 
               
               
                   
                   
                 TCGAAGCTCTTCTGACCCCGAACACGACGGTTATTGCTGAAACCGGTGACTCTTGGTTCAATGCTCAGCG 
               
               
                   
                   
                 CATGAAGCTCCCGAACGGTGCTCGCGTTGAATATGAAATGCAGTGGGGTCACATCGGTTGGTCCGTTCCT 
               
               
                   
                   
                 GCCGCCTTCGGTTATGCCGTCGGTGCTCCGGAACGTCGCAACATCCTCATGGTTGGTGATGGTTCCTTCC 
               
               
                   
                   
                 AGCTGACGGCTCAGGAAGTCGCTCAGATGGTTCGCCTGAAACTGCCGGTTATCATCTTCTTGATCAATAA 
               
               
                   
                   
                 CTATGGTTACACCATCGAAGTTATGATCCATGATGGTCCGTACAACAACATCAAGAACTGGGATTATGCC 
               
               
                   
                   
                 GGTCTGATGGAAGTGTTCAACGGTAACGGTGGTTATGACAGCGGTGCTGGTAAAGGCCTGAAGGCTAAAA 
               
               
                   
                   
                 CCGGTGGCGAACTGGCAGAAGCTATCAAGGTTGCTCTGGCAAACACCGACGGCCCAACCCTGATCGAATG 
               
               
                   
                   
                 CTTCATCGGTCGTGAAGACTGCACTGAAGAATTGGTCAAATGGGGTAAGCGCGTTGCTGCCGCCAACAGC 
               
               
                   
                   
                 CGTAAGCCTGTTAACAAGCTCCTCTAG 
               
               
                   
               
               
                 46 
                 Pdc( Z.   
                 MSYTVGTYLAERLVQIGLKHHFAVAGDYNLVLLDNLLLNKNMEQVYCCNELNCGESAEGYARAKGAAAAV 
               
               
                   
                   mobilis ) 
                 VTYSVGALSAFDAIGGAYAENLPVILISGAPNNNDHAAGHVLHHALGKTDYHYQLEMAKNITAAAEAIYT 
               
               
                   
                 protein 
                 PEEAPAKIDHVIKTALREKKPVYLEIACNIASMPCAAPGPASALENDEASDEASLNAAVEETLKFIANRD 
               
               
                   
                 sequence 
                 KVAVLVGSKLRAAGAEEAAVKFADALGGAVATMAAAKSFFPEENPHYIGTSWGEVSYPGVEKTMKEADAV 
               
               
                   
                   
                 IALAPVENDYSTTGWTDIPDPKKLVLAEPRSVVVNGVRFPSVHLKDYLTRLAQKVSKKTGALDFFKSLNA 
               
               
                   
                   
                 GELKKAAPADPSAPLVNAEIARQVEALLTPNTTVIAETGDSWENAQRMKLPNGARVEYEMQWGHIGWSVP 
               
               
                   
                   
                 AAFGYAVGAPERRNILMVGDGSFQLTAQEVAQMVRLKLPVIIFLINNYGYTIEVMIHDGPYNNIKNWDYA 
               
               
                   
                   
                 GLMEVFNGNGGYDSGAGKGLKAKTGGELAEAIKVALANTDGPTLIECFIGREDCTEELVKWGKRVAAANS 
               
               
                   
                   
                 RKPVNKLL 
               
               
                   
               
               
                 47 
                 carboxylic 
                 ATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGACGAGCAGTCGACCC 
               
               
                   
                 acid 
                 GCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGA 
               
               
                   
                 reductase 
                 CGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACGGTGACCGC 
               
               
                   
                 amplified 
                 CCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGTGACGCGTCTGCTGC 
               
               
                   
                 from 
                 CGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCA 
               
               
                   
                 
                   Mycobacterium 
                 
                 CAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTG 
               
               
                   
                 
                   smegmatis 
                 
                 ACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACCGGTCAGCC 
               
               
                   
                   
                 GGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAATACCTCGACCTCGC 
               
               
                   
                   
                 AGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATCACCCCGAGGTCGAC 
               
               
                   
                   
                 GACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGG 
               
               
                   
                   
                 ACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGATCAGCGCCT 
               
               
                   
                   
                 CGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACACCGAGGCGATGGTG 
               
               
                   
                   
                 GCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGTCAACTTCATGCCGC 
               
               
                   
                   
                 TCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACC 
               
               
                   
                   
                 GGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCCTGGTTCCG 
               
               
                   
                   
                 CGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCACGCAGGGCGCCGACG 
               
               
                   
                   
                 AACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGG 
               
               
                   
                   
                 ATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATC 
               
               
                   
                   
                 GTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCCACCGGTGA 
               
               
                   
                   
                 TCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCCTACCCGCGTGGCGA 
               
               
                   
                   
                 ACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTC 
               
               
                   
                   
                 GACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGG 
               
               
                   
                   
                 ACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAGGCGGTGTT 
               
               
                   
                   
                 CTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTTTCCTTCTGGCCGTG 
               
               
                   
                   
                 GTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGC 
               
               
                   
                   
                 TGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGA 
               
               
                   
                   
                 GCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCAAAGACCGC 
               
               
                   
                   
                 TACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCAGTTGCGCGAACTGC 
               
               
                   
                   
                 GGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACGATCCTCGGCACCGG 
               
               
                   
                   
                 GAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCG 
               
               
                   
                   
                 AACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCACCAACCTCG 
               
               
                   
                   
                 CCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGTTTCACCACCGTGCA 
               
               
                   
                   
                 CGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCGACGCCGAAACGCTC 
               
               
                   
                   
                 CGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCT 
               
               
                   
                   
                 GGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTCATCACGAT 
               
               
                   
                   
                 CGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACACCGATCCCGAGTTG 
               
               
                   
                   
                 TCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACATCGGCGACCCGAATC 
               
               
                   
                   
                 TGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCT 
               
               
                   
                   
                 GGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGGTGATCAAG 
               
               
                   
                   
                 CTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGCCATGGGGATCCCCG 
               
               
                   
                   
                 ACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGCGGATACGCCAACGG 
               
               
                   
                   
                 CTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTG 
               
               
                   
                   
                 GCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCCAGACATGT 
               
               
                   
                   
                 TCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTACATCGGAGACGGTGA 
               
               
                   
                   
                 GCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCG 
               
               
                   
                   
                 CAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGT 
               
               
                   
                   
                 TCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGGGTGCGTCG 
               
               
                   
                   
                 GTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGCCGCTGCTGCACGCG 
               
               
                   
                   
                 TTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCA 
               
               
                   
                   
                 CCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCT 
               
               
                   
                   
                 GCGTGAGTTCGGTCTGATCTGA 
               
               
                   
               
               
                 48 
                 codon- 
                 ATGCACCATCACCACCATCATGGAGGCGGACAGCAACTGACCGATCAAAGCAAAGAACTGGACTTCAAGA 
               
               
                   
                 optimized 
                 GCGAGACGTACAAAGACGCCTATAGCCGCATTAACGCGATCGTCATTGAAGGCGAACAAGAGGCGCATGA 
               
               
                   
                 hexahistidine- 
                 AAACTACATCACCCTGGCGCAGCTGCTGCCTGAGAGCCACGACGAACTGATTCGCCTGAGCAAAATGGAG 
               
               
                   
                 tagged 
                 AGCCGTCACAAGAAAGGTTTTGAGGCGTGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCAATTTGCGA 
               
               
                   
                 
                   Nostoc 
                 
                 AGGAGTTCTTTAGCGGTCTGCACCAGAATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTCACTTGTTT 
               
               
                   
                 
                   punctiforme 
                 
                 GTTGATCCAGAGCCTGATTATTGAATGCTTTGCTATTGCGGCGTACAACATTTACATTCCGGTCGCCGAT 
               
               
                   
                 
                   adm. 
                 
                 GACTTTGCGCGTAAAATCACGGAAGGTGTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGGTGAAGTGT 
               
               
                   
                   
                 GGTTGAAGGAACATTTTGCGGAATCTAAAGCCGAATTGGAACTGGCAAATCGCCAGAACCTGCCGATCGT 
               
               
                   
                   
                 TTGGAAGATGCTGAACCAAGTGGAAGGTGATGCACATACGATGGCGATGGAGAAGGACGCATTGGTTGAG 
               
               
                   
                   
                 GACTTTATGATTCAGTATGGCGAAGCACTGTCCAATATCGGTTTCAGCACCCGTGATATCATGCGTCTGA 
               
               
                   
                   
                 GCGCCTATGGCCTGATCGGTGCCTAA 
               
               
                   
               
               
                 49 
                 codon- 
                 ATGGAGTGGAAACCAAAACCGAAACTGCCTCAGCTGCTGGATGACCACTTCGGTCTGCACGGCCTGGTTT 
               
               
                   
                 optimized 
                 TCCGTCGTACCTTCGCTATCCGTTCTTACGAAGTCGGCCCTGATCGCTCCACCTCCATCCTGGCGGTAAT 
               
               
                   
                 
                   Umbellularia 
                 
                 GAACCACATGCAGGAAGCAACTCTGAACCATGCGAAAAGCGTAGGTATCCTGGGCGATGGTTTCGGCACT 
               
               
                   
                 
                   californica 
                 
                 ACTCTGGAGATGTCCAAACGTGATCTGATGTGGGTTGTTCGCCGTACCCATGTCGCGGTTGAACGCTACC 
               
               
                   
                 fatB m  (without 
                 CGACCTGGGGCGATACGGTTGAAGTGGAATGCTGGATCGGCGCGTCCGGCAACAACGGCATGCGTCGCGA 
               
               
                   
                 leader 
                 TTTCCTGGTTCGCGATTGTAAGACGGGCGAGATTCTGACCCGTTGCACGTCCCTGAGCGTTCTGATGAAT 
               
               
                   
                 sequence). 
                 ACCCGTACCCGTCGTCTGAGCACCATCCCGGACGAAGTTCGCGGTGAAATTGGCCCGGCATTCATCGATA 
               
               
                   
                   
                 ACGTTGCAGTAAAAGACGATGAAATCAAGAAACTGCAGAAACTGAATGACTCTACCGCGGACTACATCCA 
               
               
                   
                   
                 GGGTGGTCTGACCCCGCGCTGGAACGACCTGGACGTGAACCAGCACGTCAACAACCTGAAATACGTAGCT 
               
               
                   
                   
                 TGGGTATTCGAAACGGTCCCGGATTCTATCTTCGAATCTCACCACATCAGCTCCTTCACCCTGGAATACC 
               
               
                   
                   
                 GTCGTGAGTGTACCCGTGACTCCGTTCTGCGCTCTCTGACCACGGTATCCGGCGGTAGCTCTGAAGCCGG 
               
               
                   
                   
                 TCTGGTTTGCGATCACCTGCTGCAGCTGGAAGGCGGCAGCGAGGTTCTGCGTGCTCGTACTGAGTGGCGT 
               
               
                   
                   
                 CCGAAGCTGACTGACTCTTTCCGCGGCATCTCTGTTATCCCGGCAGAGCCTCGTGTGTAA 
               
               
                   
               
               
                 50 
                 codon- 
                 ATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGA 
               
               
                   
                 Optimized  E.   
                 ACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAA 
               
               
                   
                   coli  entD. 
                 AACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCG 
               
               
                   
                   
                 GCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTA 
               
               
                   
                   
                 CCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGC 
               
               
                   
                   
                 ACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTC 
               
               
                   
                   
                 AGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATG 
               
               
                   
                   
                 CGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGAT 
               
               
                   
                   
                 GTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGA 
               
               
                   
               
               
                 51 
                 plasmid 
                 TAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTT 
               
               
                   
                 pAQ4::P(cpcB)- 
                 GAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA 
               
               
                   
                 Nhistag_adm 
                 TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTA 
               
               
                   
                 (Npu)-ErmC 
                 TCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCC 
               
               
                   
                   
                 AGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCAT 
               
               
                   
                   
                 TCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAG 
               
               
                   
                   
                 TGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA 
               
               
                   
                   
                 CCTGGAACGCTGTTTTTCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATG 
               
               
                   
                   
                 CTTGATGGTCGGAAGTGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTG 
               
               
                   
                   
                 GCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAGCGATAGATTG 
               
               
                   
                   
                 TCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATT 
               
               
                   
                   
                 TAATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATATTCTTCCTTTTTCAATATTATTGAAGCATT 
               
               
                   
                   
                 TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTCA 
               
               
                   
                   
                 GTGTTACAACCAATTAACCAATTCTGAACATTATCGCGAGCCCATTTATACCTGAATATGGCTCATAACA 
               
               
                   
                   
                 CCCCTTGTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACG 
               
               
                   
                   
                 CCGTAGCGCCGATGGTAGTGTGGGGACTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACG 
               
               
                   
                   
                 AAAGGCTCAGTCGAAAGACTGGGCCTTTCGCCCGGGCTAATTAGGGGGTGTCGCCCTTTACACGTACTTA 
               
               
                   
                   
                 GTCGCTGAAGGCCTCACTGGCCCCTGCAGGGATGGTGGAATGCTGGTTATCTGGTGGGGATTAAGTGGTG 
               
               
                   
                   
                 TTTTACTAAAGCTTGAACAACTCAAGAAAGATTATATTCGCAATAACTGCCAATAATCCCAGCATCTTGA 
               
               
                   
                   
                 GAAAATCCAGCAAACCGGGGGCAAAACACCAGCAAGAAGCCAGCAGACTATCACCAAATCCCCAGCGTAC 
               
               
                   
                   
                 AGCTAGAAATAACTGAGCAGTTGTATTCAATTACCTTCTGGTCAAGCCGAGGAAATTTCCCCACACCTTA 
               
               
                   
                   
                 TACACCTCTGGAAGGTTTTTTTGACGAAGCGCAAAATATCCACAATCGGCTGGGGACTTCTTCTGTCAGA 
               
               
                   
                   
                 AAATGGCAGAAATTTTTGAATGTGTTGGCGATCGCCCTCATCAATGATTATTAGAGAACTTTTGTCCCTG 
               
               
                   
                   
                 ATGTTGGGAATACTCTTGATGACAATTGTGATTGCTCAAAGAAGAAAGAAATTTGGAGTAAATCTCTAAA 
               
               
                   
                   
                 AGGGGACTGAAATATTTGTATGGTCAGCATGACCACTGAAATGGAGAGAAGTCTAAGACAGTAGATGTCT 
               
               
                   
                   
                 TAGATATAAGCCTCATTAGAAGCCATGCCATAAAACAGATTTTGTGGATGAAACAACTTGAAATAGTTCA 
               
               
                   
                   
                 GTTGTAGACCATGTTATAAACATTTATTCTTAACACAGTGACACATTAATGACTCATATATCCGTCCAAA 
               
               
                   
                   
                 AAAAACTAAAATGTTTGTAAATTTAGTTTTGCGGCCGCGTCGACTTCGTTATAAAATAAACTTAACAAAT 
               
               
                   
                   
                 CTATACCCACCTGTAGAGAAGAGTCCCTGAATATCAAAATGGTGGGATAAAAAGCTCAAAAAGGAAAGTA 
               
               
                   
                   
                 GGCTGTGGTTCCCTAGGCAACAGTCTTCCCTACCCCACTGGAAACTAAAAAAACGAGAAAAGTTCGCACC 
               
               
                   
                   
                 GAACATCAATTGCATAATTTTAGCCCTAAAACATAAGCTGAACGAAACTGGTTGTCTTCCCTTCCCAATC 
               
               
                   
                   
                 CAGGACAATCTGAGAATCCCCTGCAACATTACTTAACAAAAAAGCAGGAATAAAATTAACAAGATGTAAC 
               
               
                   
                   
                 AGACATAAGTCCCATCACCGTTGTATAAAGTTAACTGTGGGATTGCAAAAGCATTCAAGCCTAGGCGCTG 
               
               
                   
                   
                 AGCTGTTTGAGCATCCCGGTGGCCCTTGTCGCTGCCTCCGTGTTTCTCCCTGGATTTATTTAGGTAATAT 
               
               
                   
                   
                 CTCTCATAAATCCCCGGGTAGTTAACGAAAGTTAATGGAGATCAGTAACAATAACTCTAGGGTCATTACT 
               
               
                   
                   
                 TTGGACTCCCTCAGTTTATCCGGGGGAATTGTGTTTAAGAAAATCCCAACTCATAAAGTCAAGTAGGAGA 
               
               
                   
                   
                 TTAATCATATGCACCATCACCACCATCATGGAGGCGGACAGCAACTGACCGATCAAAGCAAAGAACTGGA 
               
               
                   
                   
                 CTTCAAGAGCGAGACGTACAAAGACGCCTATAGCCGCATTAACGCGATCGTCATTGAAGGCGAACAAGAG 
               
               
                   
                   
                 GCGCATGAAAACTACATCACCCTGGCGCAGCTGCTGCCTGAGAGCCACGACGAACTGATTCGCCTGAGCA 
               
               
                   
                   
                 AAATGGAGAGCCGTCACAAGAAAGGTTTTGAGGCGTGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCA 
               
               
                   
                   
                 ATTTGCGAAGGAGTTCTTTAGCGGTCTGCACCAGAATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTC 
               
               
                   
                   
                 ACTTGTTTGTTGATCCAGAGCCTGATTATTGAATGCTTTGCTATTGCGGCGTACAACATTTACATTCCGG 
               
               
                   
                   
                 TCGCCGATGACTTTGCGCGTAAAATCACGGAAGGTGTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGG 
               
               
                   
                   
                 TGAAGTGTGGTTGAAGGAACATTTTGCGGAATCTAAAGCCGAATTGGAACTGGCAAATCGCCAGAACCTG 
               
               
                   
                   
                 CCGATCGTTTGGAAGATGCTGAACCAAGTGGAAGGTGATGCACATACGATGGCGATGGAGAAGGACGCAT 
               
               
                   
                   
                 TGGTTGAGGACTTTATGATTCAGTATGGCGAAGCACTGTCCAATATCGGTTTCAGCACCCGTGATATCAT 
               
               
                   
                   
                 GCGTCTGAGCGCCTATGGCCTGATCGGTGCCTAAGAGCTCCTCGAGGAATTCGGTTTTCCGTCCTGTCTT 
               
               
                   
                   
                 GATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAA 
               
               
                   
                   
                 AATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATTTGCCATTTACTAGTTTTAA 
               
               
                   
                   
                 TTAACGTGCTATAATTATACTAATTTTATAAGGAGGAAAAAATATGGGCATTTTTAGTATTTTTGTAATC 
               
               
                   
                   
                 AGCACAGTTCATTATCAACCAAACAAAAAATAAGTGGTTATAATGAATCGTTAATAAGCAAAATTCATAT 
               
               
                   
                   
                 AACCAAATTAAAGAGGGTTATAATGAACGAGAAAAATATAAAACACAGTCAAAACTTTATTACTTCAAAA 
               
               
                   
                   
                 CATAATATAGATAAAATAATGACAAATATAAGATTAAATGAACATGATAATATCTTTGAAATCGGCTCAG 
               
               
                   
                   
                 GAAAAGGCCATTTTACCCTTGAATTAGTAAAGAGGTGTAATTTCGTAACTGCCATTGAAATAGACCATAA 
               
               
                   
                   
                 ATTATGCAAAACTACAGAAAATAAACTTGTTGATCACGATAATTTCCAAGTTTTAAACAAGGATATATTG 
               
               
                   
                   
                 CAGTTTAAATTTCCTAAAAACCAATCCTATAAAATATATGGTAATATACCTTATAACATAAGTACGGATA 
               
               
                   
                   
                 TAATACGCAAAATTGTTTTTGATAGTATAGCTAATGAGATTTATTTAATCGTGGAATACGGGTTTGCTAA 
               
               
                   
                   
                 AAGATTATTAAATACAAAACGCTCATTGGCATTACTTTTAATGGCAGAAGTTGATATTTCTATATTAAGT 
               
               
                   
                   
                 ATGGTTCCAAGAGAATATTTTCATCCTAAACCTAAAGTGAATAGCTCACTTATCAGATTAAGTAGAAAAA 
               
               
                   
                   
                 AATCAAGAATATCACACAAAGATAAACAAAAGTATAATTATTTCGTTATGAAATGGGTTAACAAAGAATA 
               
               
                   
                   
                 CAAGAAAATATTTACAAAAAATCAATTTAACAATTCCTTAAAACATGCAGGAATTGACGATTTAAACAAT 
               
               
                   
                   
                 ATTAGCTTTGAACAATTCTTATCTCTTTTCAATAGCTATAAATTATTTAATAAGTAAGTTAAGGGATGCA 
               
               
                   
                   
                 TAAACTGCATCCCTTAACTTGTTTTTCGTGTGCCTATTTTTTGTGGCGCGCCCAGTTTCCTTTACTGGCC 
               
               
                   
                   
                 CTAAAGTCGCTGTGGCTAGGGTTCCGAAGGGGCATTATTGGCTCGCGGCTTTACAACCTTGATAAGGAGA 
               
               
                   
                   
                 GAGATGACAGTTTTTTTTCTCTTTTGCTTAGTAAAACAGCAAATTTAAGGCATGTTAAAGAGCAGTAGAA 
               
               
                   
                   
                 CGAAATGGTTGAGCCGGCCTCGATACACTCAATTAACTACTAATAGCTTCAATAAATTTTGGGACGATTG 
               
               
                   
                   
                 AAGCTATTTTTTTGAAAATCAACTCTTAATATCTCCTGTCTCAAAAGAGTTAATTGCTAAACAAAAGCCA 
               
               
                   
                   
                 GTTTCAGCGAAAAATCTAGAGTTTTATAGGTTCGTTCTCAGTACAGGACAAAAAGTTTGAAAAGGATAGA 
               
               
                   
                   
                 GGGAGAGGGTTTGATGGAAATAAGCACAAATCAATCAAGCCCTCATGAATCAGATTAGCGAAATTCGCCG 
               
               
                   
                   
                 CCAATTGCGACCTCATCTCGGATGGCATGGAGCCAGACTGTCATTTATCGCCCTCTTCCTGGTGGCACTG 
               
               
                   
                   
                 TTCCGAGCAAAAACCGTCAATCTCGCCAAACTCGCCACCGTCTGGGGAGGCAATGCAGCAGAAGAGTCTA 
               
               
                   
                   
                 ATTACAAACGCATGCAGCGATTCTTTCAGTCCTTTGACGTCAACATGGACAAAATCGCCAGGATGGTAAT 
               
               
                   
                   
                 GAATATCGCGGCTATCCCGCAACCTTGGGTCTTAAGCATCGACCGCACCAACGGCCGGCCTACATGGCCC 
               
               
                   
                   
                 GTCAATCGAAGGGCGACACAAAATTTATTCTAAATGCATAATAAATACTGATAACATCTTATAGTTTGTA 
               
               
                   
                   
                 TTATATTTTGTATTATCGTTGACATGTATAATTTTGATATCAAAAACTGATTTTCCCTTTATTATTTTCG 
               
               
                   
                   
                 AGATTTATTTTCTTAATTCTCTTTAACAAACTAGAAATATTGTATATACAAAAAATCATAAATAATAGAT 
               
               
                   
                   
                 GAATAGTTTAATTATAGGTGTTCATCAATCGAAAAAGCAACGTATCTTATTTAAAGTGCGTTGCTTTTTT 
               
               
                   
                   
                 CTCATTTATAAGGTTAAATAATTCTCATATATCAAGCAAAGTGACAGGCGCCCTTAAATATTCTGACAAA 
               
               
                   
                   
                 TGCTCTTTCCCTAAACTCCCCCCATAAAAAAACCCGCCGAAGCGGGTTTTTACGTTATTTGCGGATTAAC 
               
               
                   
                   
                 GATTACTCGTTATCAGAACCGCCCAGGGGGCCCGAGCTTAAGACTGGCCGTCGTTTTACAACACAGAAAG 
               
               
                   
                   
                 AGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTCCCTA 
               
               
                   
                   
                 CTCTCGCCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAG 
               
               
                   
                   
                 CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAA 
               
               
                   
                   
                 AGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCT 
               
               
                   
                   
                 GACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG 
               
               
                   
                   
                 CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 
               
               
                   
                   
                 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTC 
               
               
                   
                   
                 GTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 
               
               
                   
                   
                 ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAG 
               
               
                   
                   
                 CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGGCTAACTACGGCTACACTAGAAGA 
               
               
                   
                   
                 ACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG 
               
               
                   
                   
                 GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGG 
               
               
                   
                   
                 ATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGACGCGCGCGTAACTCAC 
               
               
                   
                   
                 GTTAAGGGATTTTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCTTT 
               
               
                   
               
               
                 52 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTA 
               
               
                   
                 pAQ3::P(nir07)- 
                 CGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 fatB m -carB- 
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA 
               
               
                   
                 entD-SpecR. 
                 GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                   
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT 
               
               
                   
                   
                 GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG 
               
               
                   
                   
                 GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT 
               
               
                   
                   
                 CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC 
               
               
                   
                   
                 GGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAAC 
               
               
                   
                   
                 TGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGT 
               
               
                   
                   
                 GTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC 
               
               
                   
                   
                 GCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATAAATGAGAAAAAAGC 
               
               
                   
                   
                 AACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAACACCTATAATTAAACTATTCATCTATTA 
               
               
                   
                   
                 TTTATGATTTTTTGTATATACAATATTTCTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATA 
               
               
                   
                   
                 ATAAAGGGAAAATCAGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCGCTGAACCTGCAGGC 
               
               
                   
                   
                 GAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAACCCGTCGCCATTGACCCAGAACCGCGCA 
               
               
                   
                   
                 AAGAACGGGAAAAAATTGATCTCGATCTGGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAA 
               
               
                   
                   
                 AGTGAAGTTAGCCGATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGACCTCTTCAAGGATG 
               
               
                   
                   
                 AAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTAAATCGTTCCTGACCGGACTCGCGGAAAA 
               
               
                   
                   
                 AGGCTACGGTGACACCCAACTGAAGGCGATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGAT 
               
               
                   
                   
                 GTCCTGACTTGGGTTGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGACCAATACATTCGAGA 
               
               
                   
                   
                 AGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCATCGAAATCAAATACCAAACCGTTAATGAA 
               
               
                   
                   
                 GGTTTAGTGATCTTGGGTCAGGATATCGGTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAG 
               
               
                   
                   
                 ATGTGGCATAAAAAAGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCTTGTAGCAATTGC 
               
               
                   
                   
                 TACTAAAAACTGCGATCGCTGCTGAAATGAGCTGGAATTTTGTCCCTCTCAGCTCAAAAAGTATCAATGA 
               
               
                   
                   
                 TTACTTAATGTTTGTTCTGCGCAAACTTCTTGCAGAACATGCATGATTTACAAAAAGTTGTAGTTTCTGT 
               
               
                   
                   
                 TACCAATTGCGAATCGAGAACTGCCTAATCTGCCGAGTATGCGATCCTTTAGCAGGAGGAAAACCATATG 
               
               
                   
                   
                 GAGTGGAAACCAAAACCGAAACTGCCTCAGCTGCTGGATGACCACTTCGGTCTGCACGGCCTGGTTTTCC 
               
               
                   
                   
                 GTCGTACCTTCGCTATCCGTTCTTACGAAGTCGGCCCTGATCGCTCCACCTCCATCCTGGCGGTAATGAA 
               
               
                   
                   
                 CCACATGCAGGAAGCAACTCTGAACCATGCGAAAAGCGTAGGTATCCTGGGCGATGGTTTCGGCACTACT 
               
               
                   
                   
                 CTGGAGATGTCCAAACGTGATCTGATGTGGGTTGTTCGCCGTACCCATGTCGCGGTTGAACGCTACCCGA 
               
               
                   
                   
                 CCTGGGGCGATACGGTTGAAGTGGAATGCTGGATCGGCGCGTCCGGCAACAACGGCATGCGTCGCGATTT 
               
               
                   
                   
                 CCTGGTTCGCGATTGTAAGACGGGCGAGATTCTGACCCGTTGCACGTCCCTGAGCGTTCTGATGAATACC 
               
               
                   
                   
                 CGTACCCGTCGTCTGAGCACCATCCCGGACGAAGTTCGCGGTGAAATTGGCCCGGCATTCATCGATAACG 
               
               
                   
                   
                 TTGCAGTAAAAGACGATGAAATCAAGAAACTGCAGAAACTGAATGACTCTACCGCGGACTACATCCAGGG 
               
               
                   
                   
                 TGGTCTGACCCCGCGCTGGAACGACCTGGACGTGAACCAGCACGTCAACAACCTGAAATACGTAGCTTGG 
               
               
                   
                   
                 GTATTCGAAACGGTCCCGGATTCTATCTTCGAATCTCACCACATCAGCTCCTTCACCCTGGAATACCGTC 
               
               
                   
                   
                 GTGAGTGTACCCGTGACTCCGTTCTGCGCTCTCTGACCACGGTATCCGGCGGTAGCTCTGAAGCCGGTCT 
               
               
                   
                   
                 GGTTTGCGATCACCTGCTGCAGCTGGAAGGCGGCAGCGAGGTTCTGCGTGCTCGTACTGAGTGGCGTCCG 
               
               
                   
                   
                 AAGCTGACTGACTCTTTCCGCGGCATCTCTGTTATCCCGGCAGAGCCTCGTGTGTAAGAGCTCGAGGAGG 
               
               
                   
                   
                 TTTTTACAATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGACGAGCA 
               
               
                   
                   
                 GTCGACCCGCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCC 
               
               
                   
                   
                 GTGGTCGACGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACG 
               
               
                   
                   
                 GTGACCGCCCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGTGACGCG 
               
               
                   
                   
                 TCTGCTGCCGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCC 
               
               
                   
                   
                 CTGCGCCACAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCG 
               
               
                   
                   
                 ATTACCTGACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACC 
               
               
                   
                   
                 GGTCAGCCGGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAATACCTC 
               
               
                   
                   
                 GACCTCGCAGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATCACCCCG 
               
               
                   
                   
                 AGGTCGACGACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCAC 
               
               
                   
                   
                 CACCCTGGACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGAT 
               
               
                   
                   
                 CAGCGCCTCGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACACCGAGG 
               
               
                   
                   
                 CGATGGTGGCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGTCAACTT 
               
               
                   
                   
                 CATGCCGCTCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTAC 
               
               
                   
                   
                 TTCGTACCGGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCC 
               
               
                   
                   
                 TGGTTCCGCGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCACGCAGGG 
               
               
                   
                   
                 CGCCGACGAACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTG 
               
               
                   
                   
                 ATCACCGGATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCG 
               
               
                   
                   
                 CACACATCGTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCC 
               
               
                   
                   
                 ACCGGTGATCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCCTACCCG 
               
               
                   
                   
                 CGTGGCGAACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCACCGCGA 
               
               
                   
                   
                 GCGTCTTCGACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGT 
               
               
                   
                   
                 GTACGTGGACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAG 
               
               
                   
                   
                 GCGGTGTTCTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTTTCCTTC 
               
               
                   
                   
                 TGGCCGTGGTGGTCCCGACGCCGGAG&lt;+GCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGC 
               
               
                   
                   
                 CGACTCGCTGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTC 
               
               
                   
                   
                 GAGACCGAGCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCA 
               
               
                   
                   
                 AAGACCGCTACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCAGTTGCG 
               
               
                   
                   
                 CGAACTGCGGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACGATCCTC 
               
               
                   
                   
                 GGCACCGGGAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGA 
               
               
                   
                   
                 CACTTTCGAACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCAC 
               
               
                   
                   
                 CAACCTCGCCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGTTTCACC 
               
               
                   
                   
                 ACCGTGCACGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCGACGCCG 
               
               
                   
                   
                 AAACGCTCCGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGC 
               
               
                   
                   
                 CAACGGCTGGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTC 
               
               
                   
                   
                 ATCACGATCGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACACCGATC 
               
               
                   
                   
                 CCGAGTTGTCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACATCGGCGA 
               
               
                   
                   
                 CCCGAATCTGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCG 
               
               
                   
                   
                 GCAGCGCTGGTCAACCACGTGCTCCCCTACCGG&lt;+GCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGG 
               
               
                   
                   
                 TGATCAAGCTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGCCATGGG 
               
               
                   
                   
                 GATCCCCGACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGCGGATAC 
               
               
                   
                   
                 GCCAACGGCTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGC 
               
               
                   
                   
                 TGCCCGTGGCGACOTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCC 
               
               
                   
                   
                 AGACATGTTCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTACATCGGA 
               
               
                   
                   
                 GACGGTGAGCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCACGACGC 
               
               
                   
                   
                 TCGGCGCGCAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCT 
               
               
                   
                   
                 GGATGTGTTCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGG 
               
               
                   
                   
                 GTGCGTCGGTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGCCGCTGC 
               
               
                   
                   
                 TGCACGCGTTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCACGCCGC 
               
               
                   
                   
                 GGTGCGCACCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATA 
               
               
                   
                   
                 CGCGATCTGCGTGAGTTCGGTCTGATCTGAGGTACCCACAAGGAGGTTTTTACAATGAAAACGACCCACA 
               
               
                   
                   
                 CCAGCTTA&lt;XATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGAACTTTTGTGAACAAGA 
               
               
                   
                   
                 CCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAAAACTGAACATCTGGCC 
               
               
                   
                   
                 GGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCGGCCATTGGTGAACTGC 
               
               
                   
                   
                 GTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTACCGCGTTGGCGGTTGT 
               
               
                   
                   
                 GTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGCACGCGAGCTGACGGAC 
               
               
                   
                   
                 AACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCC 
               
               
                   
                   
                 TGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATGCGGGCTTCCTGGATTA 
               
               
                   
                   
                 TCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGATGTTTGCCGTCCATTGG 
               
               
                   
                   
                 CAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGAGAATTCGGTTTTCCGTCCTGTCTTG 
               
               
                   
                   
                 ATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAAA 
               
               
                   
                   
                 ATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATTTGCCATTTACTAGTTTTTAA 
               
               
                   
                   
                 TTAACCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACT 
               
               
                   
                   
                 GTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTT 
               
               
                   
                   
                 GATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGG 
               
               
                   
                   
                 GAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAAC 
               
               
                   
                   
                 CGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGA 
               
               
                   
                   
                 TTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAA 
               
               
                   
                   
                 ACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACA 
               
               
                   
                   
                 TCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGC 
               
               
                   
                   
                 AGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGC 
               
               
                   
                   
                 GTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGC 
               
               
                   
                   
                 TAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTAC 
               
               
                   
                   
                 GTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCA 
               
               
                   
                   
                 ATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAG 
               
               
                   
                   
                 AAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAA 
               
               
                   
                   
                 GGTAGTCGGCAAATAATGTCTAACAATTCGTTCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAAGC 
               
               
                   
                   
                 GTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTATTATTTTTAA 
               
               
                   
                   
                 GCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAGGCGCGCCACGAGAAAGAGTTATGACA 
               
               
                   
                   
                 AATTAAAATTCTGACTCTTAGATTATTTCCAGAGAGGCTGATTTTCCCAATCTTTGGGAAAGCCTAAGTT 
               
               
                   
                   
                 TTTAGATTCTATTTCTGGATACATCTCAAAAGTTCTTTTTAAATGCTGTGCAAAATTATGCTCTGGTTTA 
               
               
                   
                   
                 ATTCTGTCTAAGAGATACTGAATACAACATAAGCCAGTGAAAATTTTACGGCTGTTTCTTTGATTAATAT 
               
               
                   
                   
                 CCTCCAATACTTCTCTAGAGAGCCATTTTCCTTTTAACCTATCAGGCAATTTAGGTGATTCTCCTAGCTG 
               
               
                   
                   
                 TATATTCCAGAGCCTTGAATGATGAGCGCAAATATTTCTAATATGCGACAAAGACCGTAACCAAGATATA 
               
               
                   
                   
                 AAAAACTTGTTAGGTAATTGGAAATGAGTATGTATTTTTTGTCGTGTCTTAGATGGTAATAAATTTGTGT 
               
               
                   
                   
                 ACATTCTAGATAACTGCCCAAAGGCGATTATCTCCAAAGCCATATATGACGGCGGTAGTAGAGGATTTGT 
               
               
                   
                   
                 GTACTTGTTTCGATAATGCCCGATAAATTCTTCTACTTTTTTAGATTGGCAATATTGAGTAATCGAATCG 
               
               
                   
                   
                 ATTAATTCTTGATGCTTCCCAGTGTCATAAAATAAACTTTTATTCAGATACCAATGAGGATCATAATCAT 
               
               
                   
                   
                 GGGAGTAGTGATAAATCATTTGAGTTCTGACTGCTACTTCTATCGACTCCGTAGCATTAAAAATAAGCAT 
               
               
                   
                   
                 TCTCAAGGATTTATCAAACTTGTATAGATTTGGCCGGCCCGTCAAAAGGGCGACACCCCATAATTAGCCC 
               
               
                   
                   
                 GGGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCA 
               
               
                   
                   
                 TGGGGAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGA 
               
               
                   
                   
                 CCACCGCGCTACTGCCGCCAGGCAAACAAGGGGTGTTATGAGCCATATTCAGGTATAAATGGGCTCGCGA 
               
               
                   
                   
                 TAATGTTCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATTTGTTTATTTTTCTAAATACATTCAAA 
               
               
                   
                   
                 TATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAATATGAGT 
               
               
                   
                   
                 ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAG 
               
               
                   
                   
                 AAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCT 
               
               
                   
                   
                 CAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTT 
               
               
                   
                   
                 CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATT 
               
               
                   
                   
                 CTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGA 
               
               
                   
                   
                 ATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGA 
               
               
                   
                   
                 CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG 
               
               
                   
                   
                 AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCG 
               
               
                   
                   
                 CAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT 
               
               
                   
                   
                 AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCG 
               
               
                   
                   
                 GTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTAT 
               
               
                   
                   
                 CTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG 
               
               
                   
                   
                 ATTAAGCATTGGT 
               
               
                   
               
               
                 53 
                 carboxylic 
                 ATGACCAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGACGAGCAGTCGACCC 
               
               
                   
                 acid 
                 GCCGCATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGA 
               
               
                   
                 reductase 
                 CGCGGCGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACGGTGACCGC 
               
               
                   
                 amplified 
                 CCGGCGCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGTGACGCGTCTGCTGC 
               
               
                   
                 from 
                 CGCGGTTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCA 
               
               
                   
                 
                   Mycobacterium 
                 
                 CAACTTCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTG 
               
               
                   
                 
                   smegmatis. 
                 
                 ACGCTGGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACCGGTCAGCC 
               
               
                   
                   
                 GGCTCGCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAATACCTCGACCTCGC 
               
               
                   
                   
                 AGTCGAATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATCACCCCGAGGTCGAC 
               
               
                   
                   
                 GACCACCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGG 
               
               
                   
                   
                 ACGCGATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGATCAGCGCCT 
               
               
                   
                   
                 CGCGATGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACACCGAGGCGATGGTG 
               
               
                   
                   
                 GCGCGGCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGTCAACTTCATGCCGC 
               
               
                   
                   
                 TCAACCACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACC 
               
               
                   
                   
                 GGAATCCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCCTGGTTCCG 
               
               
                   
                   
                 CGCGTCGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCACGCAGGGCGCCGACG 
               
               
                   
                   
                 AACTGACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGG 
               
               
                   
                   
                 ATTCGTCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATC 
               
               
                   
                   
                 GTCGACGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCCACCGGTGA 
               
               
                   
                   
                 TCGACTACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCCTACCCGCGTGGCGA 
               
               
                   
                   
                 ACTGCTGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTC 
               
               
                   
                   
                 GACCGGGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGG 
               
               
                   
                   
                 ACCGTCGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAGGCGGTGTT 
               
               
                   
                   
                 CTCCGGCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTTTCCTTCTGGCCGTG 
               
               
                   
                   
                 GTGGTCCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGC 
               
               
                   
                   
                 TGCAGCGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGA 
               
               
                   
                   
                 GCCGTTCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCAAAGACCGC 
               
               
                   
                   
                 TACGGGCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCAGTTGCGCGAACTGC 
               
               
                   
                   
                 GGCGCGCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACGATCCTCGGCACCGG 
               
               
                   
                   
                 GAGCGAGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCG 
               
               
                   
                   
                 AACCTGCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCACCAACCTCG 
               
               
                   
                   
                 CCCAACTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGTTTCACCACCGTGCA 
               
               
                   
                   
                 CGGCGCGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCGACGCCGAAACGCTC 
               
               
                   
                   
                 CGGGCCGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCT 
               
               
                   
                   
                 GGCTGGGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTCATCACGAT 
               
               
                   
                   
                 CGTGCGGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACACCGATCCCGAGTTG 
               
               
                   
                   
                 TCCCGCCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACATCGGCGACCCGAATC 
               
               
                   
                   
                 TGGGCCTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCT 
               
               
                   
                   
                 GGTCAACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGGTGATCAAG 
               
               
                   
                   
                 CTGGCCCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGCCATGGGGATCCCCG 
               
               
                   
                   
                 ACTTCGAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGCGGATACGCCAACGG 
               
               
                   
                   
                 CTACGGCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTG 
               
               
                   
                   
                 GCGACGTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCCAGACATGT 
               
               
                   
                   
                 TCACGCGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTACATCGGAGACGGTGA 
               
               
                   
                   
                 GCGCCCGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCG 
               
               
                   
                   
                 CAGCAGCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGT 
               
               
                   
                   
                 TCGTGGACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGGGTGCGTCG 
               
               
                   
                   
                 GTTCGAGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGCCGCTGCTGCACGCG 
               
               
                   
                   
                 TTCCGCGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCA 
               
               
                   
                   
                 CCGCGAAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCT 
               
               
                   
                   
                 GCGTGAGTTCGGTCTGATCTGA 
               
               
                   
               
               
                 54 
                 codon- 
                 ATGCACCATCACCACCATCATGGAGGCGGACAGCAACTGACCGATCAAAGCAAAGAACTGGACTTCAAGA 
               
               
                   
                 optimized 
                 GCGAGACGTACAAAGACGCCTATAGCCGCATTAACGCGATCGTCATTGAAGGCGAACAAGAGGCGCATGA 
               
               
                   
                 hexahistidine- 
                 AAACTACATCACCCTGGCGCAGCTGCTGCCTGAGAGCCACGACGAACTGATTCGCCTGAGCAAAATGGAG 
               
               
                   
                 tagged 
                 AGCCGTCACAAGAAAGGTTTTGAGGCGTGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCAATTTGCGA 
               
               
                   
                 
                   Nostoc 
                 
                 AGGAGTTCTTTAGCGGTCTGCACCAGAATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTCACTTGTTT 
               
               
                   
                 
                   punctiforme 
                 
                 GTTGATCCAGAGCCTGATTATTGAATGCTTTGCTATTGCGGCGTACAACATTTACATTCCGGTCGCCGAT 
               
               
                   
                 
                   adm. 
                 
                 GACTTTGCGCGTAAAATCACGGAAGGTGTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGGTGAAGTGT 
               
               
                   
                   
                 GGTTGAAGGAACATTTTGCGGAATCTAAAGCCGAATTGGAACTGGCAAATCGCCAGAACCTGCCGATCGT 
               
               
                   
                   
                 TTGGAAGATGCTGAACCAAGTGGAAGGTGATGCACATACGATGGCGATGGAGAAGGACGCATTGGTTGAG 
               
               
                   
                   
                 GACTTTATGATTCAGTATGGCGAAGCACTGTCCAATATCGGTTTCAGCACCCGTGATATCATGCGTCTGA 
               
               
                   
                   
                 GCGCCTATGGCCTGATCGGTGCCTAA 
               
               
                   
               
               
                 55 
                 codon- 
                 ATGAAAACGACCCACACCAGCTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGA 
               
               
                   
                 Optimized  E.   
                 ACTTTTGTGAACAAGACCTGTTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAA 
               
               
                   
                   coli  entD. 
                 AACTGAACATCTGGCCGGTCGCATTGCGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCG 
               
               
                   
                   
                 GCCATTGGTGAACTGCGTCAACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTA 
               
               
                   
                   
                 CCGCGTTGGCGGTTGTGTCTCGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGC 
               
               
                   
                   
                 ACGCGAGCTGACGGACAACATCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTC 
               
               
                   
                   
                 AGCCTGGCGCTGACCCTGGCATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATG 
               
               
                   
                   
                 CGGGCTTCCTGGATTATCAAATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGAT 
               
               
                   
                   
                 GTTTGCCGTCCATTGGCAGATTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGA 
               
               
                   
               
               
                 56 
                 plasmid 
                 AAAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTA 
               
               
                   
                 pAQ3::P(cpcB)- 
                 CGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTT 
               
               
                   
                 Nhistag_adm 
                 TCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAA 
               
               
                   
                 (Npu)-SpecR. 
                 GAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAG 
               
               
                   
                   
                 TGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCT 
               
               
                   
                   
                 GTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCG 
               
               
                   
                   
                 GATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA 
               
               
                   
                   
                 CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAG 
               
               
                   
                   
                 GTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTAT 
               
               
                   
                   
                 CTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGC 
               
               
                   
                   
                 GGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCA 
               
               
                   
                   
                 CATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACC 
               
               
                   
                   
                 GCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAAC 
               
               
                   
                   
                 TGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGT 
               
               
                   
                   
                 GTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCCTGGGCGGTTCTGATAACGAGTAATCGTTAATCC 
               
               
                   
                   
                 GCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAG 
               
               
                   
                   
                 AATATTTAAGGGCGCCTGTCACTTTGCTTGATATATGAGAATTATTTAACCTTATAAATGAGAAAAAAGC 
               
               
                   
                   
                 AACGCACTTTAAATAAGATACGTTGCTTTTTCGATTGATGAACACCTATAATTAAACTATTCATCTATTA 
               
               
                   
                   
                 TTTATGATTTTTTGTATATACAATATTTCTAGTTTGTTAAAGAGAATTAAGAAAATAAATCTCGAAAATA 
               
               
                   
                   
                 ATAAAGGGAAAATCAGTTTTTGATATCAAAATTATACATGTCAACGATAATACAAAATATAATACAAACT 
               
               
                   
                   
                 ATAAGATGTTATCAGTATTTATTATGCATTTAGAATAAATTTTGTGTCGCCCTTCGCTGAACCTGCAGGC 
               
               
                   
                   
                 GAGCATTTCAACGATGATGAATGGGACGGCGAACCCACTGAACCCGTCGCCATTGACCCAGAACCGCGCA 
               
               
                   
                   
                 AAGAACGGGAAAAAATTGATCTCGATCTGGAGGATGAACCAGAGGAAAACCGCAAACCGCAAAAAATCAA 
               
               
                   
                   
                 AGTGAAGTTAGCCGATGGGAAAGAGCGGGAACTCGCCCATACTCAAACCACAACTTTTTGGGATGCTGAT 
               
               
                   
                   
                 GGTAAACCCATTTCCGCCCAAGAATTTATCGAAAAGCTATTTGGCGACCTGCCCGACCTCTTCAAGGATG 
               
               
                   
                   
                 AAGCCGAACTACGCACCATCTGGGGGAAACCCGATACCCGTAAATCGTTCCTGACCGGACTCGCGGAAAA 
               
               
                   
                   
                 AGGCTACGGTGACACCCAACTGAAGGCGATCGCACGCATTGCCGAAGCGGAAAAAAGTGATGTCTATGAT 
               
               
                   
                   
                 GTCCTGACTTGGGTTGCCTACAACACCAAACCCATTAGCAGAGAAGAGCGAGTAATTAAGCATCGAGATC 
               
               
                   
                   
                 TGATTTTCTCGAAGTACACCGGAAAGCAGCAAGAATTTTTAGATTTTGTCCTAGACCAATACATTCGAGA 
               
               
                   
                   
                 AGGAGTGGAGGAACTTGATCGGGGGAAACTGCCTACCCTCATCGAAATCAAATACCAAACCGTTAATGAA 
               
               
                   
                   
                 GGTTTAGTGATCTTGGGTCAGGATATCGGTCAAGTATTCGCAGATTTTCAGGCGGATTTATATACCGAAG 
               
               
                   
                   
                 ATGTGGCATAAAAAAGGACGGCGATCGCCGGGGGCGTTGCCTGCCTTGAGCGGCCGCGTCGACTTCGTTA 
               
               
                   
                   
                 TAAAATAAACTTAACAAATCTATACCCACCTGTAGAGAAGAGTCCCTGAATATCAAAATGGTGGGATAAA 
               
               
                   
                   
                 AAGCTCAAAAAGGAAAGTAGGCTGTGGTTCCCTAGGCAACAGTCTTCCCTACCCCACTGGAAACTAAAAA 
               
               
                   
                   
                 AACGAGAAAAGTTCGCACCGAACATCAATTGCATAATTTTAGCCCTAAAACATAAGCTGAACGAAACTGG 
               
               
                   
                   
                 TTGTCTTCCCTTCCCAATCCAGGACAATCTGAGAATCCCCTGCAACATTACTTAACAAAAAAGCAGGAAT 
               
               
                   
                   
                 AAAATTAACAAGATGTAACAGACATAAGTCCCATCACCGTTGTATAAAGTTAACTGTGGGATTGCAAAAG 
               
               
                   
                   
                 CATTCAAGCCTAGGCGCTGAGCTGTTTGAGCATCCCGGTGGCCCTTGTCGCTGCCTCCGTGTTTCTCCCT 
               
               
                   
                   
                 GGATTTATTTAGGTAATATCTCTCATAAATCCCCGGGTAGTTAACGAAAGTTAATGGAGATCAGTAACAA 
               
               
                   
                   
                 TAACTCTAGGGTCATTACTTTGGACTCCCTCAGTTTATCCGGGGGAATTGTGTTTAAGAAAATCCCAACT 
               
               
                   
                   
                 CATAAAGTCAAGTAGGAGATTAATCATATGCACCATCACCACCATCATGGAGGCGGACAGCAACTGACCG 
               
               
                   
                   
                 ATCAAAGCAAAGAACTGGACTTCAAGAGCGAGACGTACAAAGACGCCTATAGCCGCATTAACGCGATCGT 
               
               
                   
                   
                 CATTGAAGGCGAACAAGAGGCGCATGAAAACTACATCACCCTGGCGCAGCTGCTGCCTGAGAGCCACGAC 
               
               
                   
                   
                 GAACTGATTCGCCTGAGCAAAATGGAGAGCCGTCACAAGAAAGGTTTTGAGGCGTGTGGCCGCAATCTGG 
               
               
                   
                   
                 CGGTGACCCCGGACCTGCAATTTGCGAAGGAGTTCTTTAGCGGTCTGCACCAGAATTTCCAGACGGCCGC 
               
               
                   
                   
                 AGCCGAGGGCAAAGTCGTCACTTGTTTGTTGATCCAGAGCCTGATTATTGAATGCTTTGCTATTGCGGCG 
               
               
                   
                   
                 TACAACATTTACATTCCGGTCGCCGATGACTTTGCGCGTAAAATCACGGAAGGTGTTGTCAAAGAGGAGT 
               
               
                   
                   
                 ATTCCCACCTGAATTTCGGTGAAGTGTGGTTGAAGGAACATTTTGCGGAATCTAAAGCCGAATTGGAACT 
               
               
                   
                   
                 GGCAAATCGCCAGAACCTGCCGATCGTTTGGAAGATGCTGAACCAAGTGGAAGGTGATGCACATACGATG 
               
               
                   
                   
                 GCGATGGAGAAGGACGCATTGGTTGAGGACTTTATGATTCAGTATGGCGAAGCACTGTCCAATATCGGTT 
               
               
                   
                   
                 TCAGCACCCGTGATATCATGCGTCTGAGCGCCTATGGCCTGATCGGTGCCTAAGAGCTCCTCGAGGAATT 
               
               
                   
                   
                 CGGTTTTCCGTCCTGTCTTGATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGTTTTT 
               
               
                   
                   
                 GTTTATTGCAAAAACAAAAAATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATT 
               
               
                   
                   
                 TGCCATTTACTAGTTTTTAATTAACCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGT 
               
               
                   
                   
                 TTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGT 
               
               
                   
                   
                 TACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAA 
               
               
                   
                   
                 CAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGT 
               
               
                   
                   
                 CATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTG 
               
               
                   
                   
                 AAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTT 
               
               
                   
                   
                 TGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCAC 
               
               
                   
                   
                 CATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGG 
               
               
                   
                   
                 CAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGA 
               
               
                   
                   
                 CAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGA 
               
               
                   
                   
                 ACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGAT 
               
               
                   
                   
                 GAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGG 
               
               
                   
                   
                 ATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACA 
               
               
                   
                   
                 GGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTAC 
               
               
                   
                   
                 GTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAATGTCTAACAATTCGTTCAAGCCGACGCCGCTTCG 
               
               
                   
                   
                 CGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAG 
               
               
                   
                   
                 TCTGCTTTTATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGAGGCGCGC 
               
               
                   
                   
                 CACGAGAAAGAGTTATGACAAATTAAAATTCTGACTCTTAGATTATTTCCAGAGAGGCTGATTTTCCCAA 
               
               
                   
                   
                 TCTTTGGGAAAGCCTAAGTTTTTAGATTCTATTTCTGGATACATCTCAAAAGTTCTTTTTAAATGCTGTG 
               
               
                   
                   
                 CAAAATTATGCTCTGGTTTAATTCTGTCTAAGAGATACTGAATACAACATAAGCCAGTGAAAATTTTACG 
               
               
                   
                   
                 GCTGTTTCTTTGATTAATATCCTCCAATACTTCTCTAGAGAGCCATTTTCCTTTTAACCTATCAGGCAAT 
               
               
                   
                   
                 TTAGGTGATTCTCCTAGCTGTATATTCCAGAGCCTTGAATGATGAGCGCAAATATTTCTAATATGCGACA 
               
               
                   
                   
                 AAGACCGTAACCAAGATATAAAAAACTTGTTAGGTAATTGGAAATGAGTATGTATTTTTTGTCGTGTCTT 
               
               
                   
                   
                 AGATGGTAATAAATTTGTGTACATTCTAGATAACTGCCCAAAGGCGATTATCTCCAAAGCCATATATGAC 
               
               
                   
                   
                 GGCGGTAGTAGAGGATTTGTGTACTTGTTTCGATAATGCCCGATAAATTCTTCTACTTTTTTAGATTGGC 
               
               
                   
                   
                 AATATTGAGTAATCGAATCGATTAATTCTTGATGCTTCCCAGTGTCATAAAATAAACTTTTATTCAGATA 
               
               
                   
                   
                 CCAATGAGGATCATAATCATGGGAGTAGTGATAAATCATTTGAGTTCTGACTGCTACTTCTATCGACTCC 
               
               
                   
                   
                 GTAGCATTAAAAATAAGCATTCTCAAGGATTTATCAAACTTGTATAGATTTGGCCGGCCCGTCAAAAGGG 
               
               
                   
                   
                 CGACACCCCATAATTAGCCCGGGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCT 
               
               
                   
                   
                 GGCAGTTCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTT 
               
               
                   
                   
                 CGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAACAAGGGGTGTTATGAGCCATATTC 
               
               
                   
                   
                 AGGTATAAATGGGCTCGCGATAATGTTCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATTTGTTTA 
               
               
                   
                   
                 TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATT 
               
               
                   
                   
                 GAAAAAGGAAGAATATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCT 
               
               
                   
                   
                 TCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG 
               
               
                   
                   
                 GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAA 
               
               
                   
                   
                 TGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACT 
               
               
                   
                   
                 CGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG 
               
               
                   
                   
                 GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTAC 
               
               
                   
                   
                 TTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCG 
               
               
                   
                   
                 CCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA 
               
               
                   
                   
                 GCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAA 
               
               
                   
                   
                 TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTAT 
               
               
                   
                   
                 TGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAG 
               
               
                   
                   
                 CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCG 
               
               
                   
                   
                 CTGAGATAGGTGCCTCACTGATTAAGCATTGGT 
               
               
                   
               
               
                 57 
                 plasmid 
                 TAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTT 
               
               
                   
                 pAQ4::P(cpcB)- 
                 GAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA 
               
               
                   
                 Nhistag_adm 
                 TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTA 
               
               
                   
                 (Npu)-ErmC. 
                 TCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCC 
               
               
                   
                   
                 AGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCAT 
               
               
                   
                   
                 TCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAG 
               
               
                   
                   
                 TGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA 
               
               
                   
                   
                 CCTGGAACGCTGTTTTTCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATG 
               
               
                   
                   
                 CTTGATGGTCGGAAGTGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTG 
               
               
                   
                   
                 GCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAGCGATAGATTG 
               
               
                   
                   
                 TCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATT 
               
               
                   
                   
                 TAATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATATTCTTCCTTTTTCAATATTATTGAAGCATT 
               
               
                   
                   
                 TATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTCA 
               
               
                   
                   
                 GTGTTACAACCAATTAACCAATTCTGAACATTATCGCGAGCCCATTTATACCTGAATATGGCTCATAACA 
               
               
                   
                   
                 CCCCTTGTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACG 
               
               
                   
                   
                 CCGTAGCGCCGATGGTAGTGTGGGGACTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACG 
               
               
                   
                   
                 AAAGGCTCAGTCGAAAGACTGGGCCTTTCGCCCGGGCTAATTAGGGGGTGTCGCCCTTTACACGTACTTA 
               
               
                   
                   
                 GTCGCTGAAGGCCTCACTGGCCCCTGCAGGGATGGTGGAATGCTGGTTATCTGGTGGGGATTAAGTGGTG 
               
               
                   
                   
                 TTTTACTAAAGCTTGAACAACTCAAGAAAGATTATATTCGCAATAACTGCCAATAATCCCAGCATCTTGA 
               
               
                   
                   
                 GAAAATCCAGCAAACCGGGGGCAAAACACCAGCAAGAAGCCAGCAGACTATCACCAAATCCCCAGCGTAC 
               
               
                   
                   
                 AGCTAGAAATAACTGAGCAGTTGTATTCAATTACCTTCTGGTCAAGCCGAGGAAATTTCCCCACACCTTA 
               
               
                   
                   
                 TACACCTCTGGAAGGTTTTTTTGACGAAGCGCAAAATATCCACAATCGGCTGGGGACTTCTTCTGTCAGA 
               
               
                   
                   
                 AAATGGCAGAAATTTTTGAATGTGTTGGCGATCGCCCTCATCAATGATTATTAGAGAACTTTTGTCCCTG 
               
               
                   
                   
                 ATGTTGGGAATACTCTTGATGACAATTGTGATTGCTCAAAGAAGAAAGAAATTTGGAGTAAATCTCTAAA 
               
               
                   
                   
                 AGGGGACTGAAATATTTGTATGGTCAGCATGACCACTGAAATGGAGAGAAGTCTAAGACAGTAGATGTCT 
               
               
                   
                   
                 TAGATATAAGCCTCATTAGAAGCCATGCCATAAAACAGATTTTGTGGATGAAACAACTTGAAATAGTTCA 
               
               
                   
                   
                 GTTGTAGACCATGTTATAAACATTTATTCTTAACACAGTGACACATTAATGACTCATATATCCGTCCAAA 
               
               
                   
                   
                 AAAAACTAAAATGTTTGTAAATTTAGTTTTGCGGCCGCGTCGACTTCGTTATAAAATAAACTTAACAAAT 
               
               
                   
                   
                 CTATACCCACCTGTAGAGAAGAGTCCCTGAATATCAAAATGGTGGGATAAAAAGCTCAAAAAGGAAAGTA 
               
               
                   
                   
                 GGCTGTGGTTCCCTAGGCAACAGTCTTCCCTACCCCACTGGAAACTAAAAAAACGAGAAAAGTTCGCACC 
               
               
                   
                   
                 GAACATCAATTGCATAATTTTAGCCCTAAAACATAAGCTGAACGAAACTGGTTGTCTTCCCTTCCCAATC 
               
               
                   
                   
                 CAGGACAATCTGAGAATCCCCTGCAACATTACTTAACAAAAAAGCAGGAATAAAATTAACAAGATGTAAC 
               
               
                   
                   
                 AGACATAAGTCCCATCACCGTTGTATAAAGTTAACTGTGGGATTGCAAAAGCATTCAAGCCTAGGCGCTG 
               
               
                   
                   
                 AGCTGTTTGAGCATCCCGGTGGCCCTTGTCGCTGCCTCCGTGTTTCTCCCTGGATTTATTTAGGTAATAT 
               
               
                   
                   
                 CTCTCATAAATCCCCGGGTAGTTAACGAAAGTTAATGGAGATCAGTAACAATAACTCTAGGGTCATTACT 
               
               
                   
                   
                 TTGGACTCCCTCAGTTTATCCGGGGGAATTGTGTTTAAGAAAATCCCAACTCATAAAGTCAAGTAGGAGA 
               
               
                   
                   
                 TTAATCATATGCACCATCACCACCATCATGGAGGCGGACAGCAACTGACCGATCAAAGCAAAGAACTGGA 
               
               
                   
                   
                 CTTCAAGAGCGAGACGTACAAAGACGCCTATAGCCGCATTAACGCGATCGTCATTGAAGGCGAACAAGAG 
               
               
                   
                   
                 GCGCATGAAAACTACATCACCCTGGCGCAGCTGCTGCCTGAGAGCCACGACGAACTGATTCGCCTGAGCA 
               
               
                   
                   
                 AAATGGAGAGCCGTCACAAGAAAGGTTTTGAGGCGTGTGGCCGCAATCTGGCGGTGACCCCGGACCTGCA 
               
               
                   
                   
                 ATTTGCGAAGGAGTTCTTTAGCGGTCTGCACCAGAATTTCCAGACGGCCGCAGCCGAGGGCAAAGTCGTC 
               
               
                   
                   
                 ACTTGTTTGTTGATCCAGAGCCTGATTATTGAATGCTTTGCTATTGCGGCGTACAACATTTACATTCCGG 
               
               
                   
                   
                 TCGCCGATGACTTTGCGCGTAAAATCACGGAAGGTGTTGTCAAAGAGGAGTATTCCCACCTGAATTTCGG 
               
               
                   
                   
                 TGAAGTGTGGTTGAAGGAACATTTTGCGGAATCTAAAGCCGAATTGGAACTGGCAAATCGCCAGAACCTG 
               
               
                   
                   
                 CCGATCGTTTGGAAGATGCTGAACCAAGTGGAAGGTGATGCACATACGATGGCGATGGAGAAGGACGCAT 
               
               
                   
                   
                 TGGTTGAGGACTTTATGATTCAGTATGGCGAAGCACTGTCCAATATCGGTTTCAGCACCCGTGATATCAT 
               
               
                   
                   
                 GCGTCTGAGCGCCTATGGCCTGATCGGTGCCTAAGAGCTCCTCGAGGAATTCGGTTTTCCGTCCTGTCTT 
               
               
                   
                   
                 GATTTTCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAA 
               
               
                   
                   
                 AATATTGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATTTGCCATTTACTAGTTTTAA 
               
               
                   
                   
                 TTAACGTGCTATAATTATACTAATTTTATAAGGAGGAAAAAATATGGGCATTTTTAGTATTTTTGTAATC 
               
               
                   
                   
                 AGCACAGTTCATTATCAACCAAACAAAAAATAAGTGGTTATAATGAATCGTTAATAAGCAAAATTCATAT 
               
               
                   
                   
                 AACCAAATTAAAGAGGGTTATAATGAACGAGAAAAATATAAAACACAGTCAAAACTTTATTACTTCAAAA 
               
               
                   
                   
                 CATAATATAGATAAAATAATGACAAATATAAGATTAAATGAACATGATAATATCTTTGAAATCGGCTCAG 
               
               
                   
                   
                 GAAAAGGCCATTTTACCCTTGAATTAGTAAAGAGGTGTAATTTCGTAACTGCCATTGAAATAGACCATAA 
               
               
                   
                   
                 ATTATGCAAAACTACAGAAAATAAACTTGTTGATCACGATAATTTCCAAGTTTTAAACAAGGATATATTG 
               
               
                   
                   
                 CAGTTTAAATTTCCTAAAAACCAATCCTATAAAATATATGGTAATATACCTTATAACATAAGTACGGATA 
               
               
                   
                   
                 TAATACGCAAAATTGTTTTTGATAGTATAGCTAATGAGATTTATTTAATCGTGGAATACGGGTTTGCTAA 
               
               
                   
                   
                 AAGATTATTAAATACAAAACGCTCATTGGCATTACTTTTAATGGCAGAAGTTGATATTTCTATATTAAGT 
               
               
                   
                   
                 ATGGTTCCAAGAGAATATTTTCATCCTAAACCTAAAGTGAATAGCTCACTTATCAGATTAAGTAGAAAAA 
               
               
                   
                   
                 AATCAAGAATATCACACAAAGATAAACAAAAGTATAATTATTTCGTTATGAAATGGGTTAACAAAGAATA 
               
               
                   
                   
                 CAAGAAAATATTTACAAAAAATCAATTTAACAATTCCTTAAAACATGCAGGAATTGACGATTTAAACAAT 
               
               
                   
                   
                 ATTAGCTTTGAACAATTCTTATCTCTTTTCAATAGCTATAAATTATTTAATAAGTAAGTTAAGGGATGCA 
               
               
                   
                   
                 TAAACTGCATCCCTTAACTTGTTTTTCGTGTGCCTATTTTTTGTGGCGCGCCCAGTTTCCTTTACTGGCC 
               
               
                   
                   
                 CTAAAGTCGCTGTGGCTAGGGTTCCGAAGGGGCATTATTGGCTCGCGGCTTTACAACCTTGATAAGGAGA 
               
               
                   
                   
                 GAGATGACAGTTTTTTTTCTCTTTTGCTTAGTAAAACAGCAAATTTAAGGCATGTTAAAGAGCAGTAGAA 
               
               
                   
                   
                 CGAAATGGTTGAGCCGGCCTCGATACACTCAATTAACTACTAATAGCTTCAATAAATTTTGGGACGATTG 
               
               
                   
                   
                 AAGCTATtTTTTTGAAAATCAACTCTTAATATCTCCTGTCTCAAAAGAGTTAATTGCTAAACAAAAGCCA 
               
               
                   
                   
                 GTTTCAGCGAAAAATCTAGAGTTTTATAGGTTCGTTCTCAGTACAGGACAAAAAGTTTGAAAAGGATAGA 
               
               
                   
                   
                 GGGAGAGGGTTTGATGGAAATAAGCACAAATCAATCAAGCCCTCATGAATCAGATTAGCGAAATTCGCCG 
               
               
                   
                   
                 CCAATTGCGACCTCATCTCGGATGGCATGGAGCCAGACTGTCATTTATCGCCCTCTTCCTGGTGGCACTG 
               
               
                   
                   
                 TTCCGAGCAAAAACCGTCAATCTCGCCAAACTCGCCACCGTCTGGGGAGGCAATGCAGCAGAAGAGTCTA 
               
               
                   
                   
                 ATTACAAACGCATGCAGCGATTCTTTCAGTCCTTTGACGTCAACATGGACAAAATCGCCAGGATGGTAAT 
               
               
                   
                   
                 GAATATCGCGGCTATCCCGCAACCTTGGGTCTTAAGCATCGACCGCACCAACGGCCGGCCTACATGGCCC 
               
               
                   
                   
                 GTCAATCGAAGGGCGACACAAAATTTATTCTAAATGCATAATAAATACTGATAACATCTTATAGTTTGTA 
               
               
                   
                   
                 TTATATTTTGTATTATCGTTGACATGTATAATTTTGATATCAAAAACTGATTTTCCCTTTATTATTTTCG 
               
               
                   
                   
                 AGATTTATTTTCTTAATTCTCTTTAACAAACTAGAAATATTGTATATACAAAAAATCATAAATAATAGAT 
               
               
                   
                   
                 GAATAGTTTAATTATAGGTGTTCATCAATCGAAAAAGCAACGTATCTTATTTAAAGTGCGTTGCTTTTTT 
               
               
                   
                   
                 CTCATTTATAAGGTTAAATAATTCTCATATATCAAGCAAAGTGACAGGCGCCCTTAAATATTCTGACAAA 
               
               
                   
                   
                 TGCTCTTTCCCTAAACTCCCCCCATAAAAAAACCCGCCGAAGCGGGTTTTTACGTTATTTGCGGATTAAC 
               
               
                   
                   
                 GATTACTCGTTATCAGAACCGCCCAGGGGGCCCGAGCTTAAGACTGGCCGTCGTTTTACAACACAGAAAG 
               
               
                   
                   
                 AGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTCCCTA 
               
               
                   
                   
                 CTCTCGCCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAG 
               
               
                   
                   
                 CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAA 
               
               
                   
                   
                 AGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCT 
               
               
                   
                   
                 GACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG 
               
               
                   
                   
                 CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 
               
               
                   
                   
                 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTC 
               
               
                   
                   
                 GTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 
               
               
                   
                   
                 ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAG 
               
               
                   
                   
                 CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGGCTAACTACGGCTACACTAGAAGA 
               
               
                   
                   
                 ACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG 
               
               
                   
                   
                 GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGG 
               
               
                   
                   
                 ATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGACGCGCGCGTAACTCAC 
               
               
                   
                   
                 GTTAAGGGATTTTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCTTT 
               
               
                   
               
               
                 58 
                 plasmid 
                 ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACT 
               
               
                   
                 pAQ7::P(nir07)- 
                 CCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGCGCTGCGATGATACCGCGA 
               
               
                   
                 carB-entD- 
                 GAACCACGCTCACCGGCTCCGGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTG 
               
               
                   
                 KanR 
                 GTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCC 
               
               
                   
                   
                 AGTTAATAGTTTGCGCAACGTTGTTGCCATCGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG 
               
               
                   
                   
                 GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGG 
               
               
                   
                   
                 TTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGC 
               
               
                   
                   
                 AGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACC 
               
               
                   
                   
                 AAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCG 
               
               
                   
                   
                 CGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT 
               
               
                   
                   
                 CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACT 
               
               
                   
                   
                 TTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACAC 
               
               
                   
                   
                 GGAAATGTTGAATACTCATATTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCAT 
               
               
                   
                   
                 GAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTCAGTGTTACAACCAATTAACCA 
               
               
                   
                   
                 ATTCTGAACATTATCGCGAGCCCATTTATACCTGAATATGGCTCATAACACCCCTTGTTTGCCTGGCGGC 
               
               
                   
                   
                 AGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG 
               
               
                   
                   
                 TGGGGACTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACT 
               
               
                   
                   
                 GGGCCTTTCGCCCGGGCTAATTATGGGGTGTCGCCCTTATTCGACTCTATAGTGAAGTTCCTATTCTCTA 
               
               
                   
                   
                 GAAAGTATAGGAACTTCTGAAGTGGGGCCTGCAGGGCCACCACAGCCAAATTCATCGTTAATGTGGACTT 
               
               
                   
                   
                 GCCGACGCCCCCTTTTCGACTAACAATCGCAATTTTTTTCATAGACATTTCCCACAGACCACATCAAATT 
               
               
                   
                   
                 ACAGCAATTGATCTAGCTGAAAGTTTAACCCACTTCCCCCCAGACCCAGAAGACCAGAGGCGCTTAAGCT 
               
               
                   
                   
                 TCCCCGAACAAACTCAACTGACCGAGGGGGAGGGAGCCGTAGCGGCGTTGGTGTTGGCGTAAATGACAGG 
               
               
                   
                   
                 CCGAGCAAAGAGCGATGAGATTTTCCCGACGATTGTCTTCGGGGATGTAATTTTTGTGGTGGACGCTTAA 
               
               
                   
                   
                 GGTTAAAACAGCCCGCAGGTGACGATCAATGCCTTTGACCTTCACATCCGACGGAATACAAACCAAGCCA 
               
               
                   
                   
                 CAGAGTTCACAGCGCCAGTCTGCATCCTCTTTTACTTGTAAGGCGATCGCCTGCCAATCATCAGAATATC 
               
               
                   
                   
                 GAGAAGAATGTTTCATCTAAACCTAGCGCCGCAAGATAATCCTGAAATCGCTACAGTATTAAAAAATTCT 
               
               
                   
                   
                 GGCCAACATCACAGCCAATACTGCGGCCGCTACTCATTAGTTAAGTGTAATGCAGAAAACGCATATTCTC 
               
               
                   
                   
                 TATTAAACTTACGCATTAATACGAGAATTTTGTAGCTACTTATACTATTTTACCTGAGATCCCGACATAA 
               
               
                   
                   
                 CCTTAGAAGTATCGAAATCGTTACATAAACATTCACACAAACCACTTGACAAATTTAGCCAATGTAAAAG 
               
               
                   
                   
                 ACTACAGTTTCTCCCCGGTTTAGTTCTAGAGTTACCTTCAGTGAAACATCGGCGGCGTGTCAGTCATTGA 
               
               
                   
                   
                 AGTAGCATAAATCAATTCAAAATACCCTGCGGGAAGGCTGCGCCAACAAAATTAAATATTTGGTTTTTCA 
               
               
                   
                   
                 CTATTAGAGCATCGATTCATTAATCAAAAACCTTACCCCCCAGCCCCCTTCCCTTGTAGGGAAGTGGGAG 
               
               
                   
                   
                 CCAAACTCCCCTCTCCGCGTCGGAGCGAAAAGTCTGAGCGGAGGTTTCCTCCGAACAGAACTTTTAAAGA 
               
               
                   
                   
                 GAGAGGGGTTGGGGGAGAGGTTCTTTCAAGATTACTAAATTGCTATCACTAGACCTCGTAGAACTAGCAA 
               
               
                   
                   
                 AGACTACGGGTGGATTGATCTTGAGCAAAAAAACTTTATGAGAACTTTAGCAGGAGGAAAACCATATGAC 
               
               
                   
                   
                 CAGCGATGTTCACGACGCCACAGACGGCGTCACCGAAACCGCACTCGACGACGAGCAGTCGACCCGCCGC 
               
               
                   
                   
                 ATCGCCGAGCTGTACGCCACCGATCCCGAGTTCGCCGCCGCCGCACCGTTGCCCGCCGTGGTCGACGCGG 
               
               
                   
                   
                 CGCACAAACCCGGGCTGCGGCTGGCAGAGATCCTGCAGACCCTGTTCACCGGCTACGGTGACCGCCCGGC 
               
               
                   
                   
                 GCTGGGATACCGCGCCCGTGAACTGGCCACCGACGAGGGCGGGCGCACCGTGACGCGTCTGCTGCCGCGG 
               
               
                   
                   
                 TTCGACACCCTCACCTACGCCCAGGTGTGGTCGCGCGTGCAAGCGGTCGCCGCGGCCCTGCGCCACAACT 
               
               
                   
                   
                 TCGCGCAGCCGATCTACCCCGGCGACGCCGTCGCGACGATCGGTTTCGCGAGTCCCGATTACCTGACGCT 
               
               
                   
                   
                 GGATCTCGTATGCGCCTACCTGGGCCTCGTGAGTGTTCCGCTGCAGCACAACGCACCGGTCAGCCGGCTC 
               
               
                   
                   
                 GCCCCGATCCTGGCCGAGGTCGAACCGCGGATCCTCACCGTGAGCGCCGAATACCTCGACCTCGCAGTCG 
               
               
                   
                   
                 AATCCGTGCGGGACGTCAACTCGGTGTCGCAGCTCGTGGTGTTCGACCATCACCCCGAGGTCGACGACCA 
               
               
                   
                   
                 CCGCGACGCACTGGCCCGCGCGCGTGAACAACTCGCCGGCAAGGGCATCGCCGTCACCACCCTGGACGCG 
               
               
                   
                   
                 ATCGCCGACGAGGGCGCCGGGCTGCCGGCCGAACCGATCTACACCGCCGACCATGATCAGCGCCTCGCGA 
               
               
                   
                   
                 TGATCCTGTACACCTCGGGTTCCACCGGCGCACCCAAGGGTGCGATGTACACCGAGGCGATGGTGGCGCG 
               
               
                   
                   
                 GCTGTGGACCATGTCGTTCATCACGGGTGACCCCACGCCGGTCATCAACGTCAACTTCATGCCGCTCAAC 
               
               
                   
                   
                 CACCTGGGCGGGCGCATCCCCATTTCCACCGCCGTGCAGAACGGTGGAACCAGTTACTTCGTACCGGAAT 
               
               
                   
                   
                 CCGACATGTCCACGCTGTTCGAGGATCTCGCGCTGGTGCGCCCGACCGAACTCGGCCTGGTTCCGCGCGT 
               
               
                   
                   
                 CGCCGACATGCTCTACCAGCACCACCTCGCCACCGTCGACCGCCTGGTCACGCAGGGCGCCGACGAACTG 
               
               
                   
                   
                 ACCGCCGAGAAGCAGGCCGGTGCCGAACTGCGTGAGCAGGTGCTCGGCGGACGCGTGATCACCGGATTCG 
               
               
                   
                   
                 TCAGCACCGCACCGCTGGCCGCGGAGATGAGGGCGTTCCTCGACATCACCCTGGGCGCACACATCGTCGA 
               
               
                   
                   
                 CGGCTACGGGCTCACCGAGACCGGCGCCGTGACACGCGACGGTGTGATCGTGCGGCCACCGGTGATCGAC 
               
               
                   
                   
                 TACAAGCTGATCGACGTTCCCGAACTCGGCTACTTCAGCACCGACAAGCCCTACCCGCGTGGCGAACTGC 
               
               
                   
                   
                 TGGTCAGGTCGCAAACGCTGACTCCCGGGTACTACAAGCGCCCCGAGGTCACCGCGAGCGTCTTCGACCG 
               
               
                   
                   
                 GGACGGCTACTACCACACCGGCGACGTCATGGCCGAGACCGCACCCGACCACCTGGTGTACGTGGACCGT 
               
               
                   
                   
                 CGCAACAACGTCCTCAAACTCGCGCAGGGCGAGTTCGTGGCGGTCGCCAACCTGGAGGCGGTGTTCTCCG 
               
               
                   
                   
                 GCGCGGCGCTGGTGCGCCAGATCTTCGTGTACGGCAACAGCGAGCGCAGTTTCCTTCTGGCCGTGGTGGT 
               
               
                   
                   
                 CCCGACGCCGGAGGCGCTCGAGCAGTACGATCCGGCCGCGCTCAAGGCCGCGCTGGCCGACTCGCTGCAG 
               
               
                   
                   
                 CGCACCGCACGCGACGCCGAACTGCAATCCTACGAGGTGCCGGCCGATTTCATCGTCGAGACCGAGCCGT 
               
               
                   
                   
                 TCAGCGCCGCCAACGGGCTGCTGTCGGGTGTCGGAAAACTGCTGCGGCCCAACCTCAAAGACCGCTACGG 
               
               
                   
                   
                 GCAGCGCCTGGAGCAGATGTACGCCGATATCGCGGCCACGCAGGCCAACCAGTTGCGCGAACTGCGGCGC 
               
               
                   
                   
                 GCGGCCGCCACACAACCGGTGATCGACACCCTCACCCAGGCCGCTGCCACGATCCTCGGCACCGGGAGCG 
               
               
                   
                   
                 AGGTGGCATCCGACGCCCACTTCACCGACCTGGGCGGGGATTCCCTGTCGGCGCTGACACTTTCGAACCT 
               
               
                   
                   
                 GCTGAGCGATTTCTTCGGTTTCGAAGTTCCCGTCGGCACCATCGTGAACCCGGCCACCAACCTCGCCCAA 
               
               
                   
                   
                 CTCGCCCAGCACATCGAGGCGCAGCGCACCGCGGGTGACCGCAGGCCGAGTTTCACCACCGTGCACGGCG 
               
               
                   
                   
                 CGGACGCCACCGAGATCCGGGCGAGTGAGCTGACCCTGGACAAGTTCATCGACGCCGAAACGCTCCGGGC 
               
               
                   
                   
                 CGCACCGGGTCTGCCCAAGGTCACCACCGAGCCACGGACGGTGTTGCTCTCGGGCGCCAACGGCTGGCTG 
               
               
                   
                   
                 GGCCGGTTCCTCACGTTGCAGTGGCTGGAACGCCTGGCACCTGTCGGCGGCACCCTCATCACGATCGTGC 
               
               
                   
                   
                 GGGGCCGCGACGACGCCGCGGCCCGCGCACGGCTGACCCAGGCCTACGACACCGATCCCGAGTTGTCCCG 
               
               
                   
                   
                 CCGCTTCGCCGAGCTGGCCGACCGCCACCTGCGGGTGGTCGCCGGTGACATCGGCGACCCGAATCTGGGC 
               
               
                   
                   
                 CTCACACCCGAGATCTGGCACCGGCTCGCCGCCGAGGTCGACCTGGTGGTGCATCCGGCAGCGCTGGTCA 
               
               
                   
                   
                 ACCACGTGCTCCCCTACCGGCAGCTGTTCGGCCCCAACGTCGTGGGCACGGCCGAGGTGATCAAGCTGGC 
               
               
                   
                   
                 CCTCACCGAACGGATCAAGCCCGTCACGTACCTGTCCACCGTGTCGGTGGCCATGGGGATCCCCGACTTC 
               
               
                   
                   
                 GAGGAGGACGGCGACATCCGGACCGTGAGCCCGGTGCGCCCGCTCGACGGCGGATACGCCAACGGCTACG 
               
               
                   
                   
                 GCAACAGCAAGTGGGCCGGCGAGGTGCTGCTGCGGGAGGCCCACGATCTGTGCGGGCTGCCCGTGGCGAC 
               
               
                   
                   
                 GTTCCGCTCGGACATGATCCTGGCGCATCCGCGCTACCGCGGTCAGGTCAACGTGCCAGACATGTTCACG 
               
               
                   
                   
                 CGACTCCTGTTGAGCCTCTTGATCACCGGCGTCGCGCCGCGGTCGTTCTACATCGGAGACGGTGAGCGCC 
               
               
                   
                   
                 CGCGGGCGCACTACCCCGGCCTGACGGTCGATTTCGTGGCCGAGGCGGTCACGACGCTCGGCGCGCAGCA 
               
               
                   
                   
                 GCGCGAGGGATACGTGTCCTACGACGTGATGAACCCGCACGACGACGGGATCTCCCTGGATGTGTTCGTG 
               
               
                   
                   
                 GACTGGCTGATCCGGGCGGGCCATCCGATCGACCGGGTCGACGACTACGACGACTGGGTGCGTCGGTTCG 
               
               
                   
                   
                 AGACCGCGTTGACCGCGCTTCCCGAGAAGCGCCGCGCACAGACCGTACTGCCGCTGCTGCACGCGTTCCG 
               
               
                   
                   
                 CGCTCCGCAGGCACCGTTGCGCGGCGCACCCGAACCCACGGAGGTGTTCCACGCCGCGGTGCGCACCGCG 
               
               
                   
                   
                 AAGGTGGGCCCGGGAGACATCCCGCACCTCGACGAGGCGCTGATCGACAAGTACATACGCGATCTGCGTG 
               
               
                   
                   
                 AGTTCGGTCTGATCTCGAGCTCGTGAGGTACCCACAAGGAGGTTTTTACAATGAAAACGACCCACACCAG 
               
               
                   
                   
                 CTTACCATTTGCCGGCCACACGTTACATTTCGTCGAATTTGATCCGGCGAACTTTTGTGAACAAGACCTG 
               
               
                   
                   
                 TTGTGGCTGCCGCATTATGCCCAGCTGCAGCACGCAGGCCGTAAGCGTAAAACTGAACATCTGGCCGGTC 
               
               
                   
                   
                 GCATT6CGGCAGTGTATGCCCTGCGCGAGTACGGCTACAAATGCGTGCCGGCCATTGGTGAACTGCGTCA 
               
               
                   
                   
                 ACCGGTTTGGCCGGCAGAAGTTTACGGTTCCATCTCCCACTGCGGTACTACCGCGTTGGCGGTTGTGTCT 
               
               
                   
                   
                 CGCCAGCCGATCGGTATTGATATTGAAGAGATATTCTCTGTCCAGACGGCACGCGAGCTGACGGACAACA 
               
               
                   
                   
                 TCATTACCCCGGCAGAGCACGAGCGTCTGGCGGACTGTGGTCTGGCGTTCAGCCTGGCGCTGACCCTGGC 
               
               
                   
                   
                 ATTCAGCGCAAAAGAGAGCGCGTTCAAGGCTTCCGAGATCCAAACCGATGCGGGCTTCCTGGATTATCAA 
               
               
                   
                   
                 ATCATCAGCTGGAACAAGCAACAGGTTATCATTCACCGTGAGAATGAGATGTTTGCCGTCCATTGGCAGA 
               
               
                   
                   
                 TTAAAGAGAAAATCGTTATCACCCTGTGCCAGCACGACTGAGAATTCGGTTTTCCGTCCTGTCTTGATTT 
               
               
                   
                   
                 TCAAGCAAACAATGCCTCCGATTTCTAATCGGAGGCATTTGTTTTTGTTTATTGCAAAAACAAAAAATAT 
               
               
                   
                   
                 TGTTACAAATTTTTACAGGCTATTAAGCCTACCGTCATAAATAATTTGCCATTTACTAGTTTTTAATTAA 
               
               
                   
                   
                 ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAA 
               
               
                   
                   
                 TGCTTCAATAATATTGAAAAAGGAAGAGTATGATTGAACAAGATGGCCTGCATGCTGGTTCTCCGGCTGC 
               
               
                   
                   
                 TTGGGTGGAACGCCTGTTTGGTTACGACTGGGCTCAGCTGACTATTGGCTGTAGCGATGCAGCGGTTTTC 
               
               
                   
                   
                 CGTCTGTCTGCACAGGGTCGTCCGGTTCTGTTTGTGAAAACCGACCTGTCCGGCGCACTGAACGAACTGC 
               
               
                   
                   
                 AGGACGAAGCGGCCCGTCTGTCCTGGCTCGCGACGACTGGTGTTCCGTGCGCGGCAGTTCTGGACGTAGT 
               
               
                   
                   
                 TACTGAAGCCGGTCGCGATTGGCTGCTGCTGGGTGAAGTTCCGGGTCAGGATCTGCTGAGCAGCCACCTC 
               
               
                   
                   
                 GCTCCGGCAGAAAAAGTTTCCATCATGGCGGACGCGATGCGCCGTCTGCACACCCTGGACCCGGCAACTT 
               
               
                   
                   
                 GCCCGTTTGACCATCAGGCTAAACACCGTATTGAACGTGCACGCACTCGTATGGAAGCGGGTCTGGTTGA 
               
               
                   
                   
                 TCAGGACGACCTGGATGAAGAGCACCAGGGCCTCGCACCGGCGGAACTGTTTGCACGTCTGAAAGCCCGC 
               
               
                   
                   
                 ATGCCGGACGGCGAAGACCTGGTGGTAACGCATGGCGACGCTTGTCTGCCAAACATTATGGTGGAAAACG 
               
               
                   
                   
                 GCCGCTTCTCTGGTTTTATTGACTGTGGCCGTCTGGGTGTAGCTGATCGCTATCAGGATATCGCCCTCGC 
               
               
                   
                   
                 TACCCGCGATATTGCAGAAGAACTGGGTGGTGAATGGGCTGACCGTTTCCTGGTGCTGTACGGTATCGCA 
               
               
                   
                   
                 GCGCCGGATTCTCAGCGCATTGCCTTCTACCGTCTGCTGGATGAGTTCTTCTAAGGCGCGCCGAAACTGC 
               
               
                   
                   
                 GCCAAGAATAGCTCACTTCAAATCAGTCACGGTTTTGTTTAGGGCTTGTCTGGCGATTTTGGTGACATAG 
               
               
                   
                   
                 ACAGTCACAGCAACAGTAGCCACAAAACCAAGAATCCGGATCGACCACTGGGCAATGGGGTTGGCGCTGG 
               
               
                   
                   
                 TGCTTTCTGTGCCGAGGGTCGCAAGATTTCCGGCCAGGGAGCCAATGTAGACATACATGATGGTGCCAGG 
               
               
                   
                   
                 GATCATCCCCACAGAGCCGAGGACATAGTCTTTTAGGGAAACGCCCGTGACCCCATAGGCATAGTTAAGC 
               
               
                   
                   
                 AGATTAAAGGGAAATACAGGTGAGAGACGCGTCAGGAGAACAATCTTCAGGCCTTCCTTGCCCACAGCTT 
               
               
                   
                   
                 CGTCGATGGCGCGAAATTTCGGGTTGTCGGCGATTTTTTGGCTCACCCATTGGCGGGCCAGATAACGACC 
               
               
                   
                   
                 CACTAGGAAAGCAGCGATCGCTCCTAGGGTTGCGCCAACAAAGACGTAAATTGATCCTAAAGCGACACCA 
               
               
                   
                   
                 AAAACAACCCCGGCTCCCAAGGTCAGAATCGACCCCGGTAGAAAAGCCACCGTCGCCACCACATAAAGCA 
               
               
                   
                   
                 CCATAAAGGCGATGGCCGGCCAAAATGAAGTGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCTATAG 
               
               
                   
                   
                 TGAGTCGAATAAGGGCGACACAAAATTTATTCTAAATGCATAATAAATACTGATAACATCTTATAGTTTG 
               
               
                   
                   
                 TATTATATTTTGTATTATCGTTGACATGTATAATTTTGATATCAAAAACTGATTTTCCCTTTATTATTTT 
               
               
                   
                   
                 CGAGATTTATTTTCTTAATTCTCTTTAACAAACTAGAAATATTGTATATACAAAAAATCATAAATAATAG 
               
               
                   
                   
                 ATGAATAGTTTAATTATAGGTGTTCATCAATCGAAAAAGCAACGTATCTTATTTAAAGTGCGTTGCTTTT 
               
               
                   
                   
                 TTCTCATTTATAAGGTTAAATAATTCTCATATATCAAGCAAAGTGACAGGCGCCCTTAAATATTCTGACA 
               
               
                   
                   
                 AATGCTCTTTCCCTAAACTCCCCCCATAAAAAAACCCGCCGAAGCGGGTTTTTACGTTATTTGCGGATTA 
               
               
                   
                   
                 ACGATTACTCGTTATCAGAACCGCCCAGGGGGCCCGAGCTTAAGACTGGCCGTCGTTTTACAACACAGAA 
               
               
                   
                   
                 AGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTCCC 
               
               
                   
                   
                 TACTCTCGCCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATC 
               
               
                   
                   
                 AGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCA 
               
               
                   
                   
                 AAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC 
               
               
                   
                   
                 CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCA 
               
               
                   
                   
                 GGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCC 
               
               
                   
                   
                 GCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG 
               
               
                   
                   
                 TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAA 
               
               
                   
                   
                 CTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATT 
               
               
                   
                   
                 AGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGGCTAACTACGGCTACACTAGAA 
               
               
                   
                   
                 GAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATC 
               
               
                   
                   
                 CGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAA 
               
               
                   
                   
                 GGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGACGCGCGCGTAACTC 
               
               
                   
                   
                 ACGTTAAGGGATTTTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCTTTT 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
               
               
               
               
               
               
               
             
               
               
               
               
               
               
               
               
             
           
               
                   
                 TABLE 2 
               
               
                   
                   
               
               
                   
                 General 
                   
                   
                   
                   
                   
                   
               
               
                   
                 Enzyme 
                 Enzyme 
                   
                   
                 Gene 
                   
                 Accession 
               
               
                   
                 Activity 
                 Activity 
                 Enzyme 
                 EC # 
                 Name 
                 Organism 
                 Number 
               
               
                   
                   
               
             
             
               
                   
               
             
          
           
               
                 1 
                 Alkane 
                 An aldehyde + 
                 alkane 
                 4.1.99.5 
                 adm 
                   Cyanothece  sp. 
                 YP_001802195 
               
               
                   
                 deformylative 
                 O2 + 2 
                 deformylative 
                   
                   
                 ATCC 51142 
               
               
                   
                 monooxygenase 
                 NADPH + 2 
                 monooxygenase 
                   
                 adm 
                 
                   Nostoc 
                 
                 YP_001865325 
               
               
                   
                 activity 
                 H+ = an (n − 1) 
                   
                   
                   
                 
                   punctiforme 
                 
               
               
                   
                   
                 alkane + 
                   
                   
                 adm 
                 
                   Prochlorococcus 
                 
                 YP_397029 
               
               
                   
                   
                 formate + H2O + 
                   
                   
                   
                   marinus  MIT 
               
               
                   
                   
                 2 NADP+ 
                   
                   
                   
                 9312 
               
               
                   
                   
                   
                   
                   
                 adm 
                 
                   Thermosynechococcus 
                 
                 NP_682103 
               
               
                   
                   
                   
                   
                   
                   
                 
                   elongatus 
                 
               
               
                   
                   
                   
                   
                   
                   
                 BP-1 
               
               
                 2 
                 Carboxylic acid 
                 An aldehyde + 
                 carboxylic acid 
                 1.2.99.6 
                 carB 
                 
                   Mycobacterium 
                 
                 YP_889972 
               
               
                   
                 reductase 
                 acceptor + 
                 reductase 
                   
                   
                   smegmatis  str. 
               
               
                   
                 activity 
                 H2O = a 
                   
                   
                   
                 MC2 155 
               
               
                   
                   
                 carboxylate + 
                   
                   
                 car 
                 
                   Nocardia 
                 
                 AAR91681 
               
               
                   
                   
                 reduced 
                   
                   
                   
                 
                   iowensis 
                 
               
               
                   
                   
                 acceptor 
                   
                   
                 fadD9 
                 
                   Mycobacterium 
                 
                 YP_001850422 
               
               
                   
                   
                   
                   
                   
                   
                   marinum  M 
               
               
                 3 
                 Phospho- 
                 CoA-[4′- 
                 phosphopanthetheinyl 
                 2.7.8.7 
                 entD 
                 
                   Escherichia coli 
                 
                 NP_415115 
               
               
                   
                 panthetheinyl 
                 phosphopantetheine] + 
                 transferase 
                   
                 sfp 
                 
                   Bacillus subtilis 
                 
                 ZP_12673024 
               
               
                   
                 transferase 
                 apo- 
                   
                   
                   
                 subsp.  subtilis   
               
               
                   
                 activity 
                 [acyl-carrier 
                   
                   
                   
                 str. SC-8 
               
               
                   
                   
                 protein] = 
               
               
                   
                   
                 adenosine 3′,5′- 
               
               
                   
                   
                 bisphosphate + 
               
               
                   
                   
                 holo-[acyl- 
               
               
                   
                   
                 carrier protein] 
               
               
                 4 
                 Thioesterase 
                 A fatty acyl- 
                 thioesterase 
                 3.1.2.14 
                 fatB2 
                 
                   Cuphea 
                 
                 AAC49269 
               
               
                   
                 activity 
                 [acyl-carrier 
                   
                   
                   
                 
                   hookeriana 
                 
               
               
                   
                   
                 protein] + H2O = 
                   
                   
                 tesA 
                 
                   Escherichia coli 
                 
                 NP_415027 
               
               
                   
                   
                 [acyl-carrier 
                   
                   
                 FatB3 
                 
                   Cocos nucifera 
                 
                 AEM72521 
               
               
                   
                   
                 protein] + a 
                   
                   
                 Ua- 
                 
                   Ulmus 
                 
                 AAB71731 
               
               
                   
                   
                 fatty acid 
                   
                   
                 FatB1 
                 
                   americana 
                 
               
               
                 5 
                 Long-chain 
                 An aldehyde + 
                 long-chain acyl- 
                 1.2.1.50 
                 acrM 
                 
                   Acinetobacter 
                 
                 BAB85476 
               
               
                   
                 acyl-CoA 
                 CoA + NADP + = 
                 CoA reductase 
                   
                   
                 sp. M-1 
               
               
                   
                 reductase 
                 an acyl-CoA + 
                   
                   
                 ucpA 
                 
                   Escherichia coli 
                 
                 NP_416921 
               
               
                   
                 activity 
                 NADPH + 
                   
                   
                 ybbO 
                 
                   Escherichia coli 
                 
                 NP_415026 
               
               
                   
                   
                 H+ 
                   
                   
                 luxC 
                 
                   Photorhabdus 
                 
                 NP_929340 
               
               
                   
                   
                   
                   
                   
                   
                 
                   luminescens 
                 
               
               
                   
                   
                   
                   
                   
                   
                 subsp.  laumondii   
               
               
                   
                   
                   
                   
                   
                   
                 TTO1 
               
               
                   
                   
                   
                   
                   
                 acr1 
                 
                   Acinetobacter 
                 
                 YP_047869 
               
               
                   
                   
                   
                   
                   
                   
                 sp. ADP-1 
               
               
                 6 
                 Long-chain fatty 
                 ATP + a long- 
                 long-chain fatty 
                 6.2.1.3 
                 fadD 
                 
                   Escherichia coli 
                 
                 NP_416319 
               
               
                   
                 acid CoA-ligase 
                 chain fatty acid + 
                 acid CoA-ligase 
                   
                 fadD 
                 
                   Synechococcus 
                 
                 YP_001733936 
               
               
                   
                 activity 
                 CoA = AMP + 
                   
                   
                   
                 
                   elongatus 
                 
               
               
                   
                   
                 diphosphate + 
                   
                   
                 TTC0079 
                 
                   Thermus 
                 
                 YP_004054 
               
               
                   
                   
                 an acyl-CoA 
                   
                   
                   
                 
                   thermophilus 
                 
               
               
                   
                   
                   
                   
                   
                   
                 HB27 
               
               
                   
               
             
          
         
       
     
         [0000]    
       
         
               
             
               
               
               
             
           
               
                 TABLE 5 
               
             
             
               
                   
               
               
                 Key to sequences on pCDF-npu plasmid 
               
             
          
           
               
                 Location (nt) 
                 Direction 
                 Feature 
               
               
                   
               
               
                  5-25 
                 forward 
                 lac operator 
               
               
                 58-63 
                 forward 
                 adm ribosome binding site 
               
               
                  71-811 
                 forward 
                 His-tagged  Nostoc punctiforme  adm 
               
               
                 882-898 
                 forward 
                 T7 promoter 
               
               
                 903-923 
                 forward 
                 lac operator 
               
               
                 954-959 
                 forward 
                 ribosome binding site 
               
               
                  965-1106 
                 forward 
                 multiple cloning site 
               
               
                 1130-1177 
                 forward 
                 T7 terminator 
               
               
                 1351-2139 
                 complement 
                 streptomycin resistance (SmR) gene 
               
               
                 2279-3017 
                 complement 
                 CloDF13 origin 
               
               
                 3227-4309 
                 complement 
                 lac repressor (lacI) 
               
               
                 4433-4449 
                 forward 
                 T7 promoter