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import gradio as gr

import urllib
import re
import sys
import warnings

import torch
import torch.nn as nn
import ipywidgets as widgets
from ipywidgets import interact, fixed

from utils.helpers import *
from utils.voxelization import processStructures
from utils.model import Model
import numpy as np

import os
import moleculekit

print(moleculekit.__version__)


def update(inp, file, mode, custom_resids, clustering_threshold):
    try:
        filepath = file.name
    except:
        print("using pdbfile")
        try:
            pdb_file = inp
            if (
                re.match(
                    "[OPQ][0-9][A-Z0-9]{3}[0-9]|[A-NR-Z][0-9]([A-Z][A-Z0-9]{2}[0-9]){1,2}",
                    pdb_file,
                ).group()
                == pdb_file
            ):
                urllib.request.urlretrieve(
                    f"https://alphafold.ebi.ac.uk/files/AF-{pdb_file}-F1-model_v2.pdb",
                    f"files/{pdb_file}.pdb",
                )
            filepath = f"files/{pdb_file}.pdb"
        except AttributeError:
            if len(inp) == 4:
                pdb_file = inp
                urllib.request.urlretrieve(
                    f"http://files.rcsb.org/download/{pdb_file.lower()}.pdb1",
                    f"files/{pdb_file}.pdb",
                )
                filepath = f"files/{pdb_file}.pdb"
            else:
                return "pdb code must be 4 letters or Uniprot code does not match", ""
    identifier = os.path.basename(filepath)
    if mode == "All residues":
        print('using all residues')
        ids = get_all_protein_resids(filepath)
    elif len(custom_resids)!=0:
        print('using listed residues', custom_resids)
        ids=get_all_resids_from_list(filepath,custom_resids.replace(","," "))
    else:
        print('using metalbinding')
        ids = get_all_metalbinding_resids(filepath)
    print(filepath)
    print(ids)
    try:
        voxels, prot_centers, prot_N, prots = processStructures(filepath, ids)
    except Exception as e:
        return "Error", f"""<div class="text-center mt-4"> Something went wrong with the voxelization, reset custom residues and other input fiels and check error message <br> <br> <code>{e}</code></div>"""
    device = torch.device("cuda" if torch.cuda.is_available() else "cpu")
    voxels.to(device)
    
    model = Model()
    model.to(device)
    model.load_state_dict(torch.load("weights/metal_0.5A_v3_d0.2_16Abox.pth", map_location=torch.device("cuda" if torch.cuda.is_available() else "cpu")))
    model.eval()
    with warnings.catch_warnings():
        warnings.filterwarnings("ignore")
        output = model(voxels)
    print(output.shape)
    prot_v = np.vstack(prot_centers)
    output_v = output.flatten().cpu().detach().numpy()
    bb = get_bb(prot_v)
    gridres = 0.5
    grid, box_N = create_grid_fromBB(bb, voxelSize=gridres)
    probability_values = get_probability_mean(grid, prot_v, output_v)
    print(probability_values.shape)
    write_cubefile(
        bb,
        probability_values,
        box_N,
        outname=f"output/metal_{identifier}.cube",
        gridres=gridres,
    )
    message = find_unique_sites(
        probability_values,
        grid,
        writeprobes=True,
        probefile=f"output/probes_{identifier}.pdb",
        threshold=7,
        p=clustering_threshold,
    )

    return message, molecule(
        filepath,
        f"output/probes_{identifier}.pdb",
        f"output/metal_{identifier}.cube",
    )



def read_mol(molpath):
    with open(molpath, "r") as fp:
        lines = fp.readlines()
    mol = ""
    for l in lines:
        mol += l
    return mol


def molecule(pdb, probes, cube):
    mol = read_mol(pdb)
    probes = read_mol(probes)
    cubefile = read_mol(cube)
    x = (
        """<!DOCTYPE html>
        <html>
        <head>    
    <meta http-equiv="content-type" content="text/html; charset=UTF-8" />
     <link rel="stylesheet" href="https://unpkg.com/flowbite@1.4.5/dist/flowbite.min.css" />
    <style>
    body{
        font-family:sans-serif
    }
.mol-container {
  width: 100%;
  height: 600px;
  position: relative;
}
.slider{
    width:80%;
    margin:0 auto
}
.slidercontainer{
    display:flex;
}
.slidercontainer > * + * {
    margin-left: 0.5rem;
}
#isovalue{
 text-align:right}
</style>
<script src="https://3Dmol.csb.pitt.edu/build/3Dmol-min.js"></script>
<script src="https://cdnjs.cloudflare.com/ajax/libs/rangeslider.js/2.3.3/rangeslider.min.js" integrity="sha512-BUlWdwDeJo24GIubM+z40xcj/pjw7RuULBkxOTc+0L9BaGwZPwiwtbiSVzv31qR7TWx7bs6OPTE5IyfLOorboQ==" crossorigin="anonymous" referrerpolicy="no-referrer"></script>
    </head>
    <body>  
    <div class="slidercontainer my-8">
    <span>Isovalue </span>
    <span id="isovalue">0.5</span>
    <input class="slider text-blue-400" type="range" id="rangeslider" min="0" max="1" step="0.05" value=0.5>
    </div>
    
    <div id="container" class="mol-container"></div>

     <div class="flex items-center justify-center my-4">
        <div class="px-4">
        <label for="sidechain" class="relative inline-flex items-center mb-4 cursor-pointer ">
            <input  id="sidechain"type="checkbox" class="sr-only peer">
            <div class="w-11 h-6 bg-gray-200 rounded-full peer peer-focus:ring-4 peer-focus:ring-blue-300 dark:peer-focus:ring-blue-800 dark:bg-gray-700 peer-checked:after:translate-x-full peer-checked:after:border-white after:absolute after:top-0.5 after:left-[2px] after:bg-white after:border-gray-300 after:border after:rounded-full after:h-5 after:w-5 after:transition-all dark:border-gray-600 peer-checked:bg-blue-600"></div>
            <span class="ml-3 text-sm font-medium text-gray-900 dark:text-gray-300">Show side chains</span>
          </label>
        </div>
        <div class="px-4">
        <label for="pdbmetal" class="relative inline-flex items-center mb-4 cursor-pointer ">
            <input  id="pdbmetal" type="checkbox" class="sr-only peer" checked>
            <div class="w-11 h-6 bg-gray-200 rounded-full peer peer-focus:ring-4 peer-focus:ring-blue-300 dark:peer-focus:ring-blue-800 dark:bg-gray-700 peer-checked:after:translate-x-full peer-checked:after:border-white after:absolute after:top-0.5 after:left-[2px] after:bg-white after:border-gray-300 after:border after:rounded-full after:h-5 after:w-5 after:transition-all dark:border-gray-600 peer-checked:bg-blue-600"></div>
            <span class="ml-3 text-sm font-medium text-gray-900 dark:text-gray-300">Show PDB metals</span>
          </label>
        </div>
        <div class="px-4">
        <label for="probes" class="relative inline-flex items-center mb-4 cursor-pointer ">
            <input  id="probes" type="checkbox" class="sr-only peer" checked>
            <div class="w-11 h-6 bg-gray-200 rounded-full peer peer-focus:ring-4 peer-focus:ring-blue-300 dark:peer-focus:ring-blue-800 dark:bg-gray-700 peer-checked:after:translate-x-full peer-checked:after:border-white after:absolute after:top-0.5 after:left-[2px] after:bg-white after:border-gray-300 after:border after:rounded-full after:h-5 after:w-5 after:transition-all dark:border-gray-600 peer-checked:bg-blue-600"></div>
            <span class="ml-3 text-sm font-medium text-gray-900 dark:text-gray-300">Show Probes</span>
          </label>
        </div>
        </div>  
        
     <div class="flex items-center justify-center my-4">
 <button type="button" class="text-gray-900 bg-white hover:bg-gray-100 border border-gray-200 focus:ring-4 focus:outline-none focus:ring-gray-100 font-medium rounded-lg text-sm px-5 py-2.5 text-center inline-flex items-center dark:focus:ring-gray-600 dark:bg-gray-800 dark:border-gray-700 dark:text-white dark:hover:bg-gray-700 mr-2 mb-2" id="download">
                    <svg class="w-6 h-6 mr-2 -ml-1" fill="none" stroke="currentColor" viewBox="0 0 24 24" xmlns="http://www.w3.org/2000/svg"><path stroke-linecap="round" stroke-linejoin="round" stroke-width="2" d="M4 16v1a3 3 0 003 3h10a3 3 0 003-3v-1m-4-4l-4 4m0 0l-4-4m4 4V4"></path></svg>
                    Download predictions
                  </button>
              </div>
            <script>
            let viewer = null;
            let voldata = null;
            let shape = null;
            let sidechain = null;
            let metal = null;
            $(document).ready(function () {
                let element = $("#container");
                let config = { backgroundColor: "white" };
                viewer = $3Dmol.createViewer( element, config );
                viewer.ui.initiateUI();
                let data = `"""
        + mol
        + """`
                viewer.addModel( data, "pdb" );
                
                let cubefile = `"""
        + cubefile
        + """`
                voldata = new $3Dmol.VolumeData(cubefile, "cube");
                shape = viewer.addIsosurface(voldata, { isoval: 0.5 , color: "blue", alpha: 0.85, smoothness: 1 });
                viewer.getModel(0).setStyle({}, {cartoon: {}}); 
                let probes =`"""
        + probes
        + """`
                viewer.addModel(probes, "pdb");
                viewer.getModel(1).setStyle({ "resn": "ZN" }, { "sphere": { }});
                viewer.getModel(1).setHoverable({}, true,
                    function (atom, viewer, event, container) {
                        if (!atom.label) {
                            atom.label = viewer.addLabel("ZN p=" + atom.pdbline.substring(55, 60), { position: atom, backgroundColor: "mintcream", fontColor: "black" });
                        }
                    },
                    function (atom, viewer) {
                        if (atom.label) {
                            viewer.removeLabel(atom.label);
                            delete atom.label;
                        }
                    }
                );
                viewer.zoomTo();
                viewer.render();
                viewer.zoom(0.8, 2000);

                $("#sidechain").change(function () {
                    if (this.checked) {
                        BB = ["C", "O", "N"]
                        viewer.getModel(0).setStyle( {"and": [{resn: ["GLY", "PRO"], invert: true},{atom: BB, invert: true},]},{stick: {hidden:false, colorscheme: "WhiteCarbon", radius: 0.3}, cartoon: {}});
                        viewer.render()
                         $("#pdbmetal").prop( "checked", false );
                    } else {
                        BB = ["C", "O", "N"]
                        viewer.getModel(0).setStyle({"and": [{resn: ["GLY", "PRO"], invert: true},{atom: BB, invert: true},]},{stick: {colorscheme: "WhiteCarbon",hidden:true, radius: 0.3}, cartoon: {}});
                        viewer.render()
                         $("#pdbmetal").prop( "checked", false );
                    }
                   
                });
                 $("#pdbmetal").change(function () {
                    if (this.checked) {
                        viewer.getModel(0).setStyle({ "resn": ["ZN","MG","NA","FE", "NI","MN","CA", "CU", "CU1"] }, { "sphere": {hidden:false}});
                        viewer.render()
                    } else {
                        viewer.getModel(0).setStyle({ "resn": ["ZN","MG","NA","FE","NI", "MN","CA", "CU", "CU1"] }, { "sphere": {hidden:true}});
                        viewer.render()
                    }
                });
                $("#probes").change(function () {
                    if (this.checked) {
                        viewer.getModel(1).setStyle({ "resn": "ZN" }, { "sphere": { }});
                        viewer.addStyle()
                        viewer.render()
                    } else {
                        viewer.getModel(1).setStyle({});
                        viewer.render()
                    }
                });

                  $("#download").click(function () {
                    download("protein.pdb", data);
                    download("metaldensity.cube", cubefile);
                    download("probes.pdb", probes);
                })

        });

         function download(filename, text) {
            var element = document.createElement("a");
            element.setAttribute("href", "data:text/plain;charset=utf-8," + encodeURIComponent(text));
            element.setAttribute("download", filename);

            element.style.display = "none";
            document.body.appendChild(element);

            element.click();

            document.body.removeChild(element);
        }

        </script>
         <script>
         $("#rangeslider").rangeslider().on("change", function (el) {
                isoval = parseFloat(el.target.value);
                $("#isovalue").text(el.target.value)
                console.log("Change isosurface to "+el.target.value)
                viewer.removeShape(shape)
                shape=viewer.addIsosurface(voldata, { isoval: parseFloat(el.target.value), color: "blue", alpha: 0.85, smoothness: 1 });
                
                viewer.render();
            });
            </script>
        </body></html>"""
    )

    return f"""<iframe style="width: 100%; height: 1000px" name="result" allow="midi; geolocation; microphone; camera; 
    display-capture; encrypted-media;" sandbox="allow-modals allow-forms 
    allow-scripts allow-same-origin allow-popups 
    allow-top-navigation-by-user-activation allow-downloads" allowfullscreen="" 
    allowpaymentrequest="" frameborder="0" srcdoc='{x}'></iframe>"""

def set_examples(example):
    n,code, resids = example
    return [
        n,
        code, 
        resids
    ]

metal3d = gr.Blocks()

with metal3d:
    gr.Markdown("# Metal3D")
    with gr.Tabs():
        with gr.TabItem("Input"):
            inp = gr.Textbox(            placeholder="PDB Code or Uniprot identifier or upload file below", label="Input molecule"
            )
            file = gr.File(file_count="single", type="file")
            
        with gr.TabItem("Settings"):
            with gr.Row():
                mode = gr.Radio(
                    ["All metalbinding residues (ASP, CYS, GLU, HIS)", "All residues"],
                    label="Residues to use for prediction",
                )
                custom_resids = gr.Textbox(placeholder="Comma separated list of residues", label="Custom residues")
            with gr.Row():
                clustering_threshold = gr.Slider(minimum=0.15,maximum=1, value=0.15,step=0.05, label="Clustering threshold")
                distance_cutoff = gr.Slider(minimum=1,maximum=10, value=7,step=1, label="Clustering distance cutoff")
        btn = gr.Button("Run")
    n = gr.Textbox(label="Label",visible=False)
    examples = gr.Dataset(components=[n,inp, custom_resids],
    samples=[["HCA2", "2CBA", ""],
                                     ["Nickel in GB1 dimer", "6F5N", ""],
                                     ["Zebrafish palmitoyltransferase ZDHHC15B PDB", "6BMS", ""],
                                     ["Human palmitoyltransferase ZDHHC23 AlphaFold", "Q8IYP9", "280,273,263,260,274,277,274,287"]],
                           )
    examples.click(fn=set_examples, inputs=examples, outputs=examples.components)
    #gr.Markdown(
    #    """ <small>Inference using CPU-only, can be quite slow for more than 20 residues. Use Colab notebook for GPU acceleration</small>
    #"""
    #)


    gr.Markdown("# Output")
    
    out = gr.Textbox(label="status")
    mol = gr.HTML()
    btn.click(fn=update, inputs=[inp, file, mode, custom_resids, clustering_threshold], outputs=[out, mol])

metal3d.launch(share=True)