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<!DOCTYPE html>
<html lang="en">
<head>
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    <title>Chemistry Batch 05 - Biochemistry - Programming Framework Analysis</title>
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    <div class="container">
        <h1>Chemistry Batch 05 - Biochemistry - Programming Framework Analysis</h1>
        
        <p>This document presents biochemistry processes analyzed using the Programming Framework methodology. Each process is represented as a computational flowchart with standardized color coding: Red for triggers/inputs, Yellow for structures/objects, Green for processing/operations, Blue for intermediates/states, and Violet for products/outputs. Yellow nodes use black text for optimal readability, while all other colors use white text.</p>

        <h2>1. Enzyme Kinetics Process</h2>
        <div class="figure">
            <div class="mermaid">
graph TD
    A1[Substrate Binding] --> B1[Enzyme Method]
    C1[Active Site] --> D1[Enzyme Substrate Complex]
    E1[Catalytic Reaction] --> F1[Enzyme Kinetics Analysis]
    
    B1 --> G1[Michaelis Menten]
    D1 --> H1[Lock and Key Model]
    F1 --> I1[Induced Fit Model]
    
    G1 --> J1[Km Determination]
    H1 --> K1[Substrate Specificity]
    I1 --> L1[Conformational Change]
    
    J1 --> M1[Vmax Calculation]
    K1 --> L1
    L1 --> N1[Transition State]
    
    M1 --> O1[Turnover Number]
    N1 --> P1[Product Formation]
    O1 --> Q1[Enzyme Kinetics Process]
    
    P1 --> R1[Enzyme Kinetics Validation]
    Q1 --> S1[Enzyme Kinetics Verification]
    R1 --> T1[Enzyme Kinetics Result]
    
    S1 --> U1[Enzyme Kinetics Analysis]
    T1 --> V1[Enzyme Kinetics Parameters]
    U1 --> W1[Enzyme Kinetics Output]
    
    V1 --> X1[Enzyme Kinetics Analysis]
    W1 --> Y1[Enzyme Kinetics Final Result]
    X1 --> Z1[Enzyme Kinetics Complete]
    
    style A1 fill:#ff6b6b,color:#fff
    style C1 fill:#ff6b6b,color:#fff
    style E1 fill:#ff6b6b,color:#fff
    
    style B1 fill:#ffd43b,color:#000
    style D1 fill:#ffd43b,color:#000
    style F1 fill:#ffd43b,color:#000
    style G1 fill:#ffd43b,color:#000
    style H1 fill:#ffd43b,color:#000
    style I1 fill:#ffd43b,color:#000
    style J1 fill:#ffd43b,color:#000
    style K1 fill:#ffd43b,color:#000
    style L1 fill:#ffd43b,color:#000
    style M1 fill:#ffd43b,color:#000
    style N1 fill:#ffd43b,color:#000
    style O1 fill:#ffd43b,color:#000
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    style Q1 fill:#ffd43b,color:#000
    style R1 fill:#ffd43b,color:#000
    style S1 fill:#ffd43b,color:#000
    style T1 fill:#ffd43b,color:#000
    style U1 fill:#ffd43b,color:#000
    style V1 fill:#ffd43b,color:#000
    style W1 fill:#ffd43b,color:#000
    style X1 fill:#ffd43b,color:#000
    style Y1 fill:#ffd43b,color:#000
    style Z1 fill:#ffd43b,color:#000
    
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    style O1 fill:#51cf66,color:#fff
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    style R1 fill:#51cf66,color:#fff
    style S1 fill:#51cf66,color:#fff
    style T1 fill:#51cf66,color:#fff
    style U1 fill:#51cf66,color:#fff
    style V1 fill:#51cf66,color:#fff
    style W1 fill:#51cf66,color:#fff
    style X1 fill:#51cf66,color:#fff
    style Y1 fill:#51cf66,color:#fff
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    style Z1 fill:#b197fc,color:#fff
            </div>
            
            <div style="margin-top: 1rem; display: flex; flex-wrap: wrap; gap: 0.5rem; justify-content: center;">
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#ff6b6b;"></span>Triggers & Inputs
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#ffd43b;"></span>Enzyme Methods
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#51cf66;"></span>Enzyme Operations
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#74c0fc;"></span>Intermediates
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#b197fc;"></span>Products
                </div>
            </div>
            
            <div class="figure-caption">
                <strong>Figure 1.</strong> Enzyme Kinetics Process. This biochemistry process visualization demonstrates enzyme catalytic mechanisms and kinetic analysis. The flowchart shows substrate inputs and active site binding, enzyme methods and complex formation, enzyme operations and catalytic reactions, intermediate results, and final enzyme kinetics outputs.
            </div>
        </div>

        <h2>2. Metabolism Process</h2>
        <div class="figure">
            <div class="mermaid">
graph TD
    A2[Metabolic Pathway] --> B2[Metabolism Method]
    C2[Energy Source] --> D2[Metabolic Regulation]
    E2[Biochemical Reaction] --> F2[Metabolism Analysis]
    
    B2 --> G2[Glycolysis]
    D2 --> H2[Krebs Cycle]
    F2 --> I2[Oxidative Phosphorylation]
    
    G2 --> J2[Glucose Breakdown]
    H2 --> K2[Citric Acid Cycle]
    I2 --> L2[Electron Transport]
    
    J2 --> M2[ATP Production]
    K2 --> L2
    L2 --> N2[NADH Formation]
    
    M2 --> O2[Energy Metabolism]
    N2 --> P2[Metabolic Flux]
    O2 --> Q2[Metabolism Process]
    
    P2 --> R2[Metabolism Validation]
    Q2 --> S2[Metabolism Verification]
    R2 --> T2[Metabolism Result]
    
    S2 --> U2[Metabolism Analysis]
    T2 --> V2[Metabolism Parameters]
    U2 --> W2[Metabolism Output]
    
    V2 --> X2[Metabolism Analysis]
    W2 --> Y2[Metabolism Final Result]
    X2 --> Z2[Metabolism Process Complete]
    
    style A2 fill:#ff6b6b,color:#fff
    style C2 fill:#ff6b6b,color:#fff
    style E2 fill:#ff6b6b,color:#fff
    
    style B2 fill:#ffd43b,color:#000
    style D2 fill:#ffd43b,color:#000
    style F2 fill:#ffd43b,color:#000
    style G2 fill:#ffd43b,color:#000
    style H2 fill:#ffd43b,color:#000
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    style X2 fill:#51cf66,color:#fff
    style Y2 fill:#51cf66,color:#fff
    style Z2 fill:#51cf66,color:#fff
    
    style Z2 fill:#b197fc,color:#fff
            </div>
            
            <div style="margin-top: 1rem; display: flex; flex-wrap: wrap; gap: 0.5rem; justify-content: center;">
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#ff6b6b;"></span>Triggers & Inputs
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#ffd43b;"></span>Metabolism Methods
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#51cf66;"></span>Metabolism Operations
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#74c0fc;"></span>Intermediates
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#b197fc;"></span>Products
                </div>
            </div>
            
            <div class="figure-caption">
                <strong>Figure 2.</strong> Metabolism Process. This biochemistry process visualization demonstrates metabolic pathways and energy production. The flowchart shows metabolic inputs and energy sources, metabolism methods and pathway regulation, metabolism operations and biochemical reactions, intermediate results, and final metabolism outputs.
            </div>
        </div>

        <h2>3. Protein Structure Process</h2>
        <div class="figure">
            <div class="mermaid">
graph TD
    A3[Amino Acid Sequence] --> B3[Protein Structure Method]
    C3[Primary Structure] --> D3[Secondary Structure]
    E3[Tertiary Structure] --> F3[Protein Structure Analysis]
    
    B3 --> G3[X Ray Crystallography]
    D3 --> H3[NMR Spectroscopy]
    F3 --> I3[Cryo EM]
    
    G3 --> J3[Alpha Helix]
    H3 --> K3[Beta Sheet]
    I3 --> L3[Protein Folding]
    
    J3 --> M3[Disulfide Bonds]
    K3 --> L3
    L3 --> N3[Hydrophobic Core]
    
    M3 --> O3[Quaternary Structure]
    N3 --> P3[Protein Function]
    O3 --> Q3[Protein Structure Process]
    
    P3 --> R3[Protein Structure Validation]
    Q3 --> S3[Protein Structure Verification]
    R3 --> T3[Protein Structure Result]
    
    S3 --> U3[Protein Structure Analysis]
    T3 --> V3[Protein Structure Parameters]
    U3 --> W3[Protein Structure Output]
    
    V3 --> X3[Protein Structure Analysis]
    W3 --> Y3[Protein Structure Final Result]
    X3 --> Z3[Protein Structure Complete]
    
    style A3 fill:#ff6b6b,color:#fff
    style C3 fill:#ff6b6b,color:#fff
    style E3 fill:#ff6b6b,color:#fff
    
    style B3 fill:#ffd43b,color:#000
    style D3 fill:#ffd43b,color:#000
    style F3 fill:#ffd43b,color:#000
    style G3 fill:#ffd43b,color:#000
    style H3 fill:#ffd43b,color:#000
    style I3 fill:#ffd43b,color:#000
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    style Z3 fill:#ffd43b,color:#000
    
    style M3 fill:#51cf66,color:#fff
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    style Q3 fill:#51cf66,color:#fff
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    style T3 fill:#51cf66,color:#fff
    style U3 fill:#51cf66,color:#fff
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    style W3 fill:#51cf66,color:#fff
    style X3 fill:#51cf66,color:#fff
    style Y3 fill:#51cf66,color:#fff
    style Z3 fill:#51cf66,color:#fff
    
    style Z3 fill:#b197fc,color:#fff
            </div>
            
            <div style="margin-top: 1rem; display: flex; flex-wrap: wrap; gap: 0.5rem; justify-content: center;">
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#ff6b6b;"></span>Triggers & Inputs
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#ffd43b;"></span>Protein Structure Methods
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#51cf66;"></span>Protein Structure Operations
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#74c0fc;"></span>Intermediates
                </div>
                <div style="display:inline-flex; align-items:center; gap:.5rem; padding:.25rem .5rem; border-radius: 999px; border: 1px solid rgba(0,0,0,.08); background:#fff;">
                    <span style="width: 12px; height: 12px; border-radius: 2px; border:1px solid rgba(0,0,0,.15); background:#b197fc;"></span>Products
                </div>
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                <strong>Figure 3.</strong> Protein Structure Process. This biochemistry process visualization demonstrates protein structure determination and analysis. The flowchart shows amino acid inputs and primary structure, protein structure methods and secondary structure, protein structure operations and tertiary structure, intermediate results, and final protein structure outputs.
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            <p><strong>Generated using the Programming Framework methodology</strong></p>
            <p>Each flowchart preserves maximum detail through optimized Mermaid configuration</p>
            <div class="contact-info">
                <p><strong>Gary Welz</strong></p>
                <p>Retired Faculty Member</p>
                <p>John Jay College, CUNY (Department of Mathematics and Computer Science)</p>
                <p>Borough of Manhattan Community College, CUNY</p>
                <p>CUNY Graduate Center (New Media Lab)</p>
                <p>Email: gwelz@jjay.cuny.edu</p>
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