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import gradio as gr
import os
import pyBigWig
from func_gradio import predict_func,make_plots
inputs = [
gr.Radio(['Micro-C', 'Hi-C (ChIA-PET)']
, label='Chromatin contact map type', info='One type of contact map is predicted for each time'),
gr.Dropdown([str(i) for i in range(1, 23)], label='Chromosome', default='1'),
gr.Number(label='Region of interest (500kb for Micro-C and 1Mb for Hi-C)', info='From'),
gr.Number(info='To', show_label=False),
gr.File(label='Processed ATAC-seq file (in .pickle format)'),
]
outputs = [
gr.Files(label='Download the results (upload the file in the "Visualize prediction results" interface for visualization)'),
]
app1 = gr.Interface(
fn=predict_func,
inputs=inputs,
outputs=outputs,
title='A computational tool to use ATAC-seq to impute epigenome, transcriptome, and high-resolution chromatin contact maps',
description= '<p>For faster inference without waiting in queue, you may duplicate the space and upgrade to GPU in settings. <a href="https://huggingface.co/spaces/drjieliu/EPCOT?duplicate=true">'
'<img style="display: inline; margin-top: 0em; margin-bottom: 0em" src="https://bit.ly/3gLdBN6" alt="Duplicate Space" /></a></p>'
'\n<a href="https://github.com/zzh24zzh/EPCOT_gradio" class="built-with svelte-1lyswbr" target="_blank" '
'style="font-size: 15px; font-color: black; font-weight:bold" rel="noreferrer">View Documentation </a>',
)
with open(os.path.abspath('data/epigenomes.txt'), 'r') as f:
epis=f.read().splitlines()
inputs1 = [
gr.File(label="Prediction file (in .npz format))"),
gr.Markdown(value='### Visualization options'),
gr.Dropdown(epis,label='Epigenome features',multiselect=True,max_choices=10,value=['CTCF','H3K4me3']),
gr.Radio(choices=['Signal p-values (archsinh)','Binding probability'], label='Type of epigenomic feature data'
, value='Signal p-values (archsinh)'),
gr.Slider(maximum=16,label='Range of values displayed on the plots',info="Choose between 0 and 16 (contact maps)",value=4),
gr.Slider(minimum=2,maximum=12,info="Choose between 2 and 12 (epigenomic feature signals)",value=4,show_label=False),
gr.Slider(minimum=2,maximum=12,info="Choose between 2 and 12 (CAGE-seq)",value=8,show_label=False),
]
outputs1 = gr.Plot(label='Plots')
app2 = gr.Interface(
fn=make_plots,
inputs=inputs1,
outputs=outputs1,
live=True
)
demo = gr.TabbedInterface([app1, app2], ["Run model", "Visualize prediction results"],
theme=gr.themes.Soft())
demo.queue(concurrency_count=1,max_size=20).launch()
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