The citizens expelled it in 1552, allying with France: this was unacceptable for Charles, who sent his general Gian Giacomo Medici to lay siege to it with a Florentine-Imperial army.
Kodanikud saatsid selle aastal 1552 välja, olles liidus Prantsusmaaga: see oli vastuvõetamatu Karlile, kes saatis oma kindrali Gian Giacomo Medici Firenze-keiserliku armeega linna piirama.