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license: mit
Out of 20,577 human proteins (from UniProt human proteome), sequences shorter than 20 amino acids or longer than 512 amino acids were removed, resulting in a set of 12,703 proteins. The uShuffle algorithm (python pacakge) was then used to shuffle these protein sequences while maintaining their triplet distribution. The sequences for which uShuffle failed to create a shuffled version were eliminated. Afterwards, h-CD-HIT algorithm (web server) was used with three subsequent filter stages at pairwise identity cutoffs of 0.9, 0.5 and 0.1, resulting in a total of 3,688 sequences.