recursal/Fanatic-Fandom huggingface dataset:
DPN1 Digest

For 100uL Reaction (Two 50uL PCR reactions):

Combine your duplicate PCR reactions into one PCR tube (with 5uL of DNA run on a gel, there should be 45uL left, so 90uL total).

You'll need FD Buffer and DPN1 from the -30, defrost the FD buffer in the 37-degree water bath and keep both on ice.

Add enough 10X fast-digest (FD) buffer to make 1X, so 10uL.

Add 1ul DPN1

Pipette up and down to mix

Incubate in 37C water bath for 15min-1hr

Then purify using the PCR purification kit above the microwave.
Clay Crime Family

Having A Sitdown

The Clay **Crime Family** is the leading crime family in New Bordeaux after the events in 1968. Lincoln Clay built the family on the ashes of the old family after the Marcano family destroyed the black mob

## Background

In 1968, after Delray Hollow based organization, Black Mob was betrayed by Salvatore Marcano, son Giorgio Marcano, associate Ritchie Doucet and the Marcano Crime Family,

Lincoln had also negotiated truces with 3 feeder factions in New Bordeaux. Like the Italian Mob run by notorious Vito Scaletta and Like the Haitian Mob run by voodoo priest Cassandra Fayette, the Irish Mob run by infamous mobster Thomas Burke

## Going After Marcano

After February 27th, 1968. Lincoln decided with Father James Ballard and John Donato to go after Salvatore Marcano and his crime family. He started by going after Sammy's old district of Delray Hollow, which Giorgio Marcano had handed over to Ritchie Doucet and his gang, The Dixie Mafia. Giorgio has got them running Heroin and other prohibited drugs against Sal's wishes as described in the mission "The Home Fires Burn". Lincoln takes over the Prostitution and Smack rackets And Kills Ritchie Doucet at Baron Saturday's Theme Park.

## Family Buisnesses

* Prostitution
* Heroin
* Union Extortion
* Contraband
* Moonshine
* Protection
* Garbage
* Gunrunning
* Blackmail
* Construction
* Auto Theft
* Smuggling
* Black Market
* Gambling
* Sex
* Cocaine
* PCP
* Fentanyl
* Weed

Underboss: John Donovan (1968-1976)
Captains: Vito Scarrone (1968-1976) · Thomas Boyne (1968-1976) · Cassandra Faloque (1968-1976)
Lieutenants: Alma Diziaraco (1968-1976) · Nicki Boyne (1968-1976) · Emmanuel Lawrenzei (1968-1976).
Racket Boss: ?
Associates: Elizabeth "Betty" Johnson. Hank McGahee. Fiona Davidson. Terrence "Terry" Daly. Bear Donnelly. Jackie DuVernay. Jennifer Moran. Clifton Jean-Baptiste. Charles Laveau.
Feeder Factions: Italian Mob · Irish Mob · Haitian Mob
Gibson Reaction

### **Set-up:**

If this is your first time doing a Gibson Reaction, refer here for more information on primer design and the basics.

Before doing Gibson Reaction, make sure you have completed the first steps of successful Cloning.

For making Gibson Master Mix and ISO Buffer please refer to here.

**You'll Need:**

ICE

DI H20: on benchtop

Gibson MM (in -80)

All Necessary Fragments and Vector

XL10 Cells from (-80)

Plates with correct antibiotic from the 4C (inside the MOM Lab)

### **Calculations:**

Our Gibson assembly master mix is 1.3X

Use 3.75uL in a 5uL
total volume (or 7.5uL in a 10uL total volume)

**First**, you will need to do calculations for your Gibson Reaction. See the Gibson Calculations Page for a step by step break down on how to calculate how much of each fragment and vector to add to your reaction. (This should be done in a PCR sized tube)

In our example on the Gibson Calculations Page, we came up with:

7.5uL of Gibson Master Mix

0.75uL of Vector

0.5uL Fragment A

0.5uL Fragment B

Water to bring the final volume to 10uL (0.75uL of water).

### **Protocol:**

1. Incubate at 50 degrees C for 15-60 min in the PCR machine.
2. During the incubation, take plates (with the correct drug: i.e. if your vector has Kanamycin resistance, you'll need plates with KanMX) and put at 37 degrees C.
3. Transform into XL10Gold Competent Cells using the Transformation Protocol
4. See https://www.neb.com/~/media/Catalog/All-Products/0AA961B294E444AFBEDD5C4A904C76E6/Datacards%20or%20Manuals/ManualE2611.pdf

### **Transformation Protocol:**

1. Add half\* of your Gibson reaction to 50uL of cells. \***don’t exceed 5uL of Gibson reaction in 50ul of**

cells

1. Incubate on Ice for 20 min.
2. While incubating, fill the 42 heat-block with water
3. After the 20 minute incubation on ice, heat shock at 42degC for 50 sec
4. Place the tube directly back on ice
5. Add 500uL SOC medium and incu-shake at 37degC for 2 hrs.
6. Plate 50uL and incubate plates at 37degC incubator overnight.
7. In the morning, remove your plates and store in the 4C.
TIRF Microscopy

***Preparing Acid Washed Glass***
Cover slips (Salmon lab)

This helps cells and polyamino acids stick to glass.
 It also helps your DIC pictures look prettier by getting rid of small particles stuck on the glass.

First separate cover
slips  from one another and place them into a glass container with a lid
containing the amount of water you'll need to add in order to make a 1M HCl
solution.  It is important that the cover slips are not sticking to each
other because you will need to use one at a time and it's easier to do this now
rather than later.

Be careful when changing
solutions not to pour out cover slips into the sink which can both block the
drain and be really irritating to some lab members.  If you do this, then
simply clean them up (be sure to rinse the sink well first if you spill HCl
along with the cover slips.

1) Heat cover slips in a
loosely covered glass beaker in 1M HCl at 50-60oC for 4-16h.

2) Cool to room
temperature

3) Rinse out 1M HCl with
ddH2O

4) Fill container with
ddH2O and sonicate in water bath for 30 mins

5) Repeat step 4

6) Repeat step 4

7) Fill container with
50% EtOH and 50% ddH2O and sonicate in water bath for 30 min

8) Fill container with
70% EtOH and 30% ddH2O and sonicate in water bath for 30 min

9) Fill container with
95% EtOH and sonicate in water bath for 30 min

10) Fill container with
95% EtOH

**Preparing Chambers**

Materials: tapes, acid
washed coverslips immersed in ethanol, Blade, burner, tweezers, slides

1. Cut tapes with each length of 3-4cm. Use a slide to prepare tapes, use tip of blades

 to almost equally divided tapes

1. Take one cut tape and stick onto another slide, doing so twice with each tape in a

 0.5cm gap

1. Use tweezer grasp a coverslip and then remove excess ethanol on it with

 kimwipe

1. Heat coverslip with tweezer for a bit over burner to remove all ethanol (will

 get a small flame and then gone)

1. Stick coverslip onto slide in the center of 2 tapes

1. Use tweezer to press the coverslip to ensure it is secured

1. Heat the slide for a bit over burner to melt and seal tape

Arrange all slides in a box
Games

## What This Is

As you might have noticed, Galaxies is made for Battleshipcraft. More specifically, Hansa Battleshipcraft. However, because the game has been removed from the App Store, many more games have become accepted as possible sources of shipbuilding. That said, each game needs a metric in order to determine what kind of ship it is such as a Destroyer or Battleship. This is a list of the games with their metrics.

## Game List

### BSC/WSC

***Toughness Scale***

* 75K Above: Mega Battleship
* 60K-74.9K: Super Battleship
* 45K-59.9K: Battleship
* 35K-44.9K: Battlecruiser
* 25K-34.9K: Heavy Cruiser
* 20K-24.9K: Light Cruiser
* 15K-19.9K: Destroyer
* 10K-14.9K: Frigate
* 1-9.9K: Corvette

### Hansa

***Toughness Scale***

* 4M Above: Mega Battleship
* 3M-3.9M: Super Battleship
* 2M-2.9M: Battleship
* 1.5M-1.9M: Battlecruiser
* 1M-1.49M: Heavy Cruiser
* 500K-999K: Light Cruiser
* 100K-499K: Destroyer
* 50K-99K: Frigate
* 1-49K: Corvette

### Reassembly

***Point Scale***

* 11,000 Above: Mega Battleship
* 8,000-10,999: Super Battleship
* 6,500-7,999: Battleship
* 5,000-6,499: Battlecruiser
* 3,500-4,999: Heavy Cruiser
* 2,500-3,499: Light Cruiser
* 1,500-2,499: Destroyer
* 750-1,500: Frigate
* 1-749: Corvette
Battleship Craft Galaxies Wiki

Battleship Craft Galaxies
Battleship Craft Galaxies is the Sister Wikia for Battleship Craft Fleets Wiki in which an Alternate Timeline has formed that has accelerated the technology Nations can create such as flying ships. For the game, visit Battleship Craft Wiki.

Wanna join a fleet? First, check out our Rules to familiarize yourself with our standards. Then come check out the Recruitment Sheet where you can find the best fleet to join.

Planning to start your own fleet though? Check out our Navy Guidelines to understand how to maintain and operate a fleet. Then, check the Star Charts to see if the place you want is available.

Wanna discuss stuff and goof around with other members? Join our Discord with this link here.

Latest Activity

News and Events
* Galaxies is still being prepared for Roleplaying.

Chatroom
<chat>
</chat>

Polls

Sister Wikias
These are the Sister Wikias of BSCG.

* BSC Navies
* BSC Roleplay
* BSC Age of Sail
* Battleshipcraft Wiki
* Warshipcraft Wiki

Shipyard
The general things you should know.

* Rules of Engagement
* Navy Guidelines
* Galaxies Staff
* What is a Navy?
* Roleplaying Characters
Sending out DNA for Sequencing

You will have 15uL total sample

You will need to label each tube (PCR sized) KM01, KM02.... etc

You need no more than 800ng of DNA in each sample.

So if your concentration is 100ng/uL --> 800ng/100ng/uL = 8uL of DNA to add

You'll also need to add 2.5uL of your primer (10uM stock)

Then you'll add water for a total of 15uL

So, as an example:

8uL DNA

2.5uL Primerr

4.5uL Water
Making de-tyrosinated Microtubule for TIRF by VASHI plus SVBP

**Part I: Got the polymerized MTs (Wild type).**

Just use normal protocol we used for microtubule polymerization.

**Part II: remove Tyrosine in C-tail of α-tubulin.**

Microtubules in BRB80 (pH 6.8)\*: 60 µl

DTT: 1 mM

PMSF: 1 mM

VASH1: SVBP:   10 nM

\*: Since we resuspend polymerized microtubules in 60 µl of BRB80 plus 20 µM Taxol, and the concentration of microtubules probably be 1mg/ml, ~10 µM.

1.     The concentration of VASH1: SVBP mixture of our stock is 22 µM, dilute it 1 to 50 with GF150 buffer (or other buffers pH close to 7), Adding 1.5 µl of dilution into 60 µl of polymerized microtubules, plus 1 mM of DTT and 1 mM of PMSF. Incubate 4 to 24 hours at ℃ in water bath.

2.     Adding 6 µl of DTT into reaction above (final concentration of DTT is 10mM) to terminate the reaction.

3.     Spin over 150 µl of sucrose cushion (25% sucrose) with Taxol in BRB80 (20 µM Taxol), 100 000x g, 10 minutes at ℃.

4.     Resuspend pellet with 60ul of BRB80-Taxol (20 µM Taxol), adding 0.6 µl of GTP and 0.6 µl of Gentamicin, keep at room temperature.

5.     Checking the de-tyrosinated microtubules on TIRF (1 to 100 dilution first). For 647 labeled tubulin, sometimes there are some debris, in this case, just take some of polymerized microtubules, spin over 150 µl of 25% sucrose (in BRB80 with 20uM taxol) on the normal centrifuge (full speed), resuspend the pellet and checking with TIRF again after dilution. (This is an optional step)
Alharis Empire

We are the Alharis Empire, also called the Sentinels. Our sole purpose is to bring havoc and destruction upon anyone who dares to cross us. We are located on a remote cluster of three solar systems about 7,200 Light Years from the Sol System. Our ships have something unique, something most other nations don't have, we have barely any weapons. Why you might ask? Well, it's plain and simple, our ships are designed specifically to ram other vessels, small or large. The damage differs from how armored the impact area is but the results are always devastating.
Olympic Stellar Navigation

Greetings, and welcome to Olympic Stellar Navigation Co. We are one of the premier interstellar passenger and freight services, and provide service to such locations as Mars, Telos, Tenelapis, and others.

### Telos

* San Eisley International Spaceport

* New Coronet Spaceport

## Members

* CEO Elijah Carson

* Chairman of the Board Zumwalt has a guy here too

* Vice Chairman

* Head of Finance

* Head of Route Planning

* Vice Head of Finance

* Head of Ship Construction and Maintenance

* Head of Marketing and Sales

## Application

Please fill out all questions when submitting your application.

* What position in our company do you want?

* What navies are you in?

* What kind of ships will you bring?

* What is the name of your character?

* How will you react with coworkers in the workplace?

## Fleet

These are the ships of our fleet, most are unarmed, and protected under international law.

The *Olympia* class starliner is our largest passenger ship. Capable of carrying 10,000 passengers, these vessels are like the Trans-Atlantic liners of the 20th century.Essentially a 3km long flying box, the Euclidian class superfreighter is a giant cargo vessel. Built by the AIF, OSN operates many of them for the AIF, hauling cargo to and from their colonies.OSN La Royale.
Harlothian Stellar Union

**Government**
 **Currency**
 **Head of State**
 **Commander Union Fleet**
 **Commander Union Army**
 **Planets**

*Welcome Traveller, to the Harlothian Stellar Union!*

This is the page for the Harlothian Stellar Union, a species spanning government in control of two habitable planets in the Damloth System. We are a peaceful race, but we will not hesitate to defend what is ours.

## Rules of the Herd

We have a few rules and guidelines that must be abided by. Failure to keep with these rules could result in exile from the Union.

### Rules

* Follow the rules. If you have problems with that, you shouldn't be in the Harlothian Fleet.

* Obey superior officers. Should be self explanatory.

* Wars and entry in to battle can only be approved by the Herdmaster or Grand Master of Fleets. Exceptions include being attacked or infringement on territory.

* Be courteous to other members. We're goat-people, not donkey-people.

* Keep ships in their proper category on the gallery.

### Guidelines

* Try to make your character name sound kinda goat like. It's nice for continuity.

* Keep in mind that these are goat people, so they don't always think like a human normally would. Also, use some non standard synonyms for objects in dialogue, ie, thinker instead of computer.

* There is a very distinct style for Harlothian ships. They tend to use vertical parts, numerous small warp nacelles, and have big engines. If you cannot make your ship follow this style in full, please go for some other kind of exotic shape, but no triangles, rectangles, or anything too much like Human, Protoss, and Sith ships. Also, if a warp nacelle on a ship has glowing parts, make them purple.

* It is heavily suggested that members can make flying ships.

## Inter-Species Relations

### Trade Partners

* Imperial Alliance of Planets: We have limited trade relations with this group of Terrans.

### Enemies

* None currently, however we distrust the destructive Protoss, and view new species with cautious suspicion.

## Application

Please fill out the following questions to join.

* Character Name (no human names please, unless your character is human)

* Types of ships you will bring

* Can you make flying ships?

* Can you follow commands and obey rules?

* Reason for joining?

### Cruisers

The *Dar'lath* class cruiser is the main heavy cruiser in the Union Fleet. A capable multirole warship, it can escort and fight larger warships, lead groups of light craft, and undertake long range independent missions. Armed with numerous mass-driver turrets, a pair of Coilgun weapons, and six torpedo tubes with numerous reloads.
### Frigates

The *Da'cal* class frigate is the primary general purpose ship in the Union Fleet. Modular in design, they can be used in numerous roles from escorting Battle Rams to exploration to scientific research.
Marcano Crime Family

Marcano Family

The **Marcano Crime Family** was a leading Mafia family in New Bordeaux before the events of 1968 and a central antagonist group in Mafia III.

## Background

The Marcano Crime Family was founded and ran by crime boss Sal Marcano and has done since their 1934 takeover from the Carillo Crime Family. The Marcano Family had maintained prominence in New Bordeaux since their 1934 takeover of the Carillo Crime Family after assassinating Giuseppe Carillo.

The families boss, Sal Marcano had started to lose his taste for crime in 1968 and decided to expand into more legitimate practices. This eventually lead to the Mafia families downfall in 1968. They decided to build a Casino across the lake.

Underboss: Giorgi Marcano
Caporegieme: Olivia Marcano · Lou Marcano · Tommy Marcano · Lucio Marcano
Lieutenants: Michael Grecco · Vito Scaletta · Roman Barbieri · Franco "Frank" Pagani · Enzo Conti · Tony Derazio
Racket Bosses: Pete Santini · Paul Simmons · Jimmy Cavar · Frankie Bernard · Harry Robicheaux · Doc Gaston · Chester Moreau · Bobby Bastian · Carl Bevers · Sonny Blue · Andy Turetto ·Roy Thibideaux · Artie Higgins · Johnny Peralta · Junior Holland · Pops Holland
Up In Flames

### Setup

Before deployment, starting with the first player, players take turns placing 1 objective token within range 1 of a play area edge.

### After Each End Phase

Each unit at range 1 of at least 1 objective token suffers {Unstable Energy} damage. Then, starting with the first player, each player places 1 objective token from the supply at range 1-{Unstable Energy} of another objective token.
Processing Clones from Whatman FTA Discs (From DGRC)

Required:

* 2 LB/antibiotic plates per disc (check resistance gene in plasmid to

 determine what antibiotic -- DGRC recommends 34mg/ml chloramphenicol 1000X
 stock)

* 1X sterile TE

* Thawed competent cells on ice\* (Do not use XL10GOLD)

* 500uL SOC medium

* Sterile microfuge tubes

1. Add 50uL sterile 1X TE to the microfuge tube with the clone disc and pipet up and down quickly twice. Remove the TE immediately and discard. Do not leave the TE on the disc for more than 2 seconds.\*\*
2. Place the tube on ice.
3. Add 50uL competent cells to tube with disc and incubate on ice for 30 minutes, vortexing for 1 second halfway and immediately returning to ice.
4. Heat shock cells/disc for 2 minutes at 37℃.
5. Transfer cells only (leave behind disc) to 500uL SOC medium and incubate with shaking for 2 hours.
6. Plate 100uL on one plate, and the remaining on another plate (should be about 450uL).
7. Incubate at 37℃ overnight.

\*DGRC recommends
DH5alpha or equivalent. Cells should have efficiency of 1 x 10^7 cfu/ug or
higher. Note that XL10 GOLD cells carry a chloramphenicol-resistance gene on
the F’ episome.

\*\* The TE step washes
off the chemical that is places on the filter to facilitate the lysis of
bacterial clones and allows the DNA to stick. Failure to wash will inhibit
transformation, but washing too long will cause DNA to elute and be lost.
Navy Guidelines

## Staff Message

All Guidelines are here to help New Members start off on a good foot and help new Navies be formed. Navies can give Members Experience for their own Navies in the future and allow users to communicate ideas and learn from each other.

## Starting a Fleet

These are some Guidelines that must be completed and followed to create a new Navy. Failure to comply with these Guidelines will result in the Navy/Nation being deleted. Exceptions to these Guidelines are granted on rare occasions.

1. All New Members ***MUST*** have to be active on BSCG in another navy for at least 1 Month. This is required to give the Member experience to run a Navy/Nation.
2. Prior to forming the Navy/Nation, you must know where it will be located. Randomly placing a Navy/Nation somewhere will not be tolerated.
3. Before creating a Navy/Nation, contact a Staff Member with your entire plan so they can determine if you can make it or not.
4. Use common sense and don't randomly make a Navy/Nation only because you were bored one day.
5. Be sure you can defend your Navy/Nation before you make it. Weak Navies/Nations will be deleted for their own good as the Navies World is tough and you will certainly be attacked if you aren't ready.

## Navy Responsibilities

When you form a Navy/Nation, you are acknowledging the following Sailor Ethics. Don't worry though as making Navies/Nations is a blast!

1. Respect all Navies/Nations, even at War.
2. Don't edit a page that isn't yours or your not a member of unless given permission.
3. Please give a description for your Navy/Nation on your page.
4. Please give instructions on How to Join your Navy/Nation on your page.
5. You are allowed to declare war on anyone.
6. Members of warring Navies/Nations are allowed to Defect.

*To Be Continued*
The Eternal Imperial Dominion of Arlotha

The **Eternal Imperial Dominion of Arlotha**, known as the **Dominion**, is a small empire located entirely within a single large overpopulated system 87,329 lightyears from Sol. Although it is a united empire, many consider it to be a dictatorship. It is ruled by Alaya Denondu, a direct descendant of Hayu Inayaa Denondu, a powerful man who united the system and became its first Emperor. Arlotha's population is 100% Arath, a humanoid alien species who are found exclusively within the tight control of the Dominion. There is no leaving, and there is no entering; Arlotha is one of the most isolated areas in the entire Milky Way Galaxy.

The quality of life in Arlotha is considerably high, that is, if you are not one of the billions of Arath enslaved and mistreated by the Dominion. The military could just come knocking at your door, take you by force, and send you to the so-called "labor planets". Defense and stability is a focal point of the government, and large measures are taken to ensure stability.

```
• **Capital:** Asrenath, Arlotha

```

```
• **Government Type:** Absolute Monarchy

```

```
• **Leader:** *His Royal Imperial Divine Majesty* Emperor Alaya Denondu

```

## Geography

The *Dominion* is located entirely within a single large system called the **Arothana System**, and is located 87,329 lightyears from Sol. The entire system revolves around a single star called *Ereth*.

* Thanedana
* Strekkellis
* Arlotha
* Koruper Alosen
* Koruper Lessenadu
* Ghunderin
* Repsotha
* Dermondos

* Population: 13,218,043,945
* Terrain: Desert
* Climate: Very Hot, Dry
* Moons: 1

**Thanedana** is a small planet and is closest to the star *Ereth*. Due to its proximity, it is scorched with immense heat making life almost impossible here. However, a small population has managed to survive. There is only one large settlement located in Thanedana, the capital city of Ereshkala. In this single city alone, 96.3% of the entire Thaned population thrives there, with the other 3.7% of the population living in smaller settlements near Ereshkala.

* Population: 73,946,854,247
* Terrain: Rainforests, Mountains
* Climate: Warm, Tropical, Humid
* Moons: 4

**Strekkellis** is one surviving example

* Population: 624,545,061,198
* Moons: 3

* Population: 435,544,762,659
* Moons: 41

* Population: 50,214,448,326
* Moons: 6

* Population: 62,405,349,583
* Moons: 15

* Population: 37,195,428,865
* Moons: 24

* Population: 89,484,685,264
* Moons: 8
Arazalean Republic

**Motto**
 **Capital**
 **Official Language**
 **Demonym**
 **Government**
**Chancellor**
 **Legislature**
 **Population**
 **Anthem**

Salutations, and welcome to the New Gallian Republic. The New Gallian Republic is the official successor to the former Third Gallian Republic from the desolate Sol System. Formed from the numerous colonisation fleets sent out by the old Republic before the fall of Earth, the New Gallian Republic seeks to continue the ideals of the old Republic and to continue to act as a shining beacon for the rest of humankind.

## Territories

### New Gallia

TBA
* **New Kingsbridge**
* New Wittenham
* New Farindon
* New Brougham
* New Richmond
* New Kingston
* New Wallinford

### Chaelum

TBA
* **Estrilla**
* Enineve
* Annavere

### Proxoria

TBA
* **Claridine**
* Soregan
* Achenna
* Anghalinda

## Leadership

* Chancellor Cornelius Wallis
* Deputy Chancellor David Hughes
* Secretary of Defence Christopher Clarkin
* Secretary of the Treasury Edward Bradford
* Secretary of Internal Affairs Peter Lloyd Asquith
* Secretary of External Affairs Christina Holdsworth
* Secretary of Research Andrew Tomlinson

## Army of the New Republic

The Army of the New Republic is the New Gallian Republic's military force.

More or less a continuation of the old Republic's army, it was further consolidated into two large entities: The Republican Army handles the planetary operations, which includes ground and atmospheric operations; while the Republican Navy is the larger one, handling all operations in space, including colonisation operations, and space battles.

The main goal of the Army of the New Republic is the defence of the Republic from any and all threats, internal or external.

### Republican Navy

#### Aerospace Fleet

* Generation A ships
* Generation B ships

*Mayflower*-class light cruiser*Argus*-class light cruiser*Eirenne*-class transport ship*Demeter*-class light battleship*Eos*-class medium battleship*Persephone*-class medium battleship*Phantasm*-class medium battleship*Deception*-class heavy battleship*Nike*-class heavy battleship*Hecate*-class dreadnought*Hera*-class dreadnought*Iris*-class carrier
<gallery>

#### Orbital Defence

*Helios*-class weapons satellite*Oblivion*-class orbital defence obelisk
Tututika

<infobox>

```
 <title source="title">
   <default>Tututika</default>
 </title>
 <image source="image">

```

 </image>
 <group>
 <header>Personal Details</header>

 <label>Also known as</label>

 <label>Status</label>

 <label>Species</label>

 <label>Age</label>

 <label>Gender</label>

 <label>Actor</label>

 </group>
 <group>
 <header>Appearances</header>

 <label>Seasons</label>

 <label>First Seen</label>

 <label>Last seen</label>

 <label>Death episode</label>

 <label>Appeared in</label>

 </group>
 <group>
 <header>Biography</header>

 <label>Occupation</label>

 <label>Date of birth</label>

 <label>Place of origin</label>

 <label>Date of death</label>

 <label>Cause of death</label>

 <label>Family</label>

 </group>
 <group>
 <header>Physical Attributes</header>

 <label>Ethnicity</label>

 <label>Hair</label>

 <label>Eye</label>

 <label>Height</label>

 <label>Weight</label>

 </group>

**Tututika** is one of the Gods in the Dandelion Dynasty book series.

## Information

She is one of the eight gods of Dara. She is the patron God of Amu.
Tazu

<infobox>

```
 <title source="title">
   <default>Tazu</default>
 </title>
 <image source="image">

```

 </image>
 <group>
 <header>Personal Details</header>

 <label>Also known as</label>

 <label>Status</label>

 <label>Species</label>

 <label>Age</label>

 <label>Gender</label>

 <label>Actor</label>

 </group>
 <group>
 <header>Appearances</header>

 <label>Seasons</label>

 <label>First Seen</label>

 <label>Last seen</label>

 <label>Death episode</label>

 <label>Appeared in</label>

 </group>
 <group>
 <header>Biography</header>

 <label>Occupation</label>

 <label>Date of birth</label>

 <label>Place of origin</label>

 <label>Date of death</label>

 <label>Cause of death</label>

 <label>Family</label>

 </group>
 <group>
 <header>Physical Attributes</header>

 <label>Ethnicity</label>

 <label>Hair</label>

 <label>Eye</label>

 <label>Height</label>

 <label>Weight</label>

 </group>

**Tazu** is one of the Gods in the Dandelion Dynasty book series.
Lincoln Clay

Possible Zodiac Sign: Matches Scorpio(but is an Aquarius)

**Lincoln Clay** is the protagonist of Mafia III, the third game in the Mafia Game Series. He is a Vietnam War veteran who starts his own criminal empire and become's the boss of the Clay Crime Family whilst seeking revenge on the Marcano Crime Family and their boss Sal Marcano after Marcano killed and eliminated his foster family the Black Mob, and left him for dead.

"*Family isn't who your born with... It's who you die for!*"
— **Lincoln Clay**

## History

Lincoln was born in January, 1945 and in 1947 he was abandoned and was taken in an orphanage in New Bordeaux. In the orphanage he met Father James, who worked there. In 1958 when the orphanage was closed he fell in with Sammy Robinson and the Black Mob in Delray Hollow and became family to him for the next 10 years. He served in Vietnam War in 1964 - 68 and came back to New Bordeaux to say goodbye to Sammy and his brother Ellis Robinson. But when Lincoln found out about a war between the Black Mob and the Haitian Mob, Lincoln decided to stay and help.

## Notable Murders

* Baka (lieutenant, Haitian Mob)
* Ritchie Doucet (boss, Dixie Mafia)
* Frank Pagani (lieutenant, Marcano Crime Family)
* Tony Derazio (lieutenant, Marcano Crime Family)
* Lou Marcano (capo, Marcano Crime Family)
* Tommy Marcano (capo, Marcano Crime Family)
* Sal Marcano (boss, Marcano Crime Family)
* Giorgi Marcano (underboss, Marcano Crime Family)
* Remy Duvall (boss, Southern Union)
* Judge Holden (Marcano Judge)
* Nino Santanelo (boss, Cuban gang)

## Optional Murders

* Vito Scaletta (underboss, Clay Crime Family)
* Thomas Burke (underboss, Clay Crime Family)
* Cassandra (underboss, Clay Crime Family)
Falcone Crime Family

"Made Man Ceremony Photo"

The **Falcone Crime Family** is one of the three Mafia Families in Empire Bay.

## History

### Background

The Falcone Crime Family was founded by Carlo Falcone after he conspired with the Vinci Crime Family to bring an end to the VInci-Moretti war by killing his boss and leader of the Moretti Crime Family, Tomaso Moretti, after this Carlo Falcone took over what was left of the crime family and started his own which what was to become the **Falcone Crime Family.**

### Drug Buisness

An modernizer, Carlo Falcone had no time for Sicilian Mafia Traditions, he realized the mob got rich during Prohibition, and that they need a similar income to stay at peace. Knowing this he got involved in the Empire Bay drug trade and quickly became the main supplier of Marijuana and Heroin in the whole city.

### Falcone-Clemente War

During his rise to the top of the Empire Bay Drug Trade, Alberto Clemente and the Clemente Crime Family tried to muscle their way into the Empire Bay drug trade by turning Frank Vinci and the Vinci Crime Family against Carlo Falcone and his faction. This resulted in Carlo Falcone ordering 2 Soldato's from his family, Vito Scaletta and Joe Barbaro to kill Alberto Clemente at the Empire Bay Arms Hotel. In the end Alberto Clemente was killed by Joe Barbaro with a Thompson 1928 in point-blank range in the head.

After the assassination of Mob-Boss Alberto Clemente he then set his sights on one person who could destroy his drug empire, Vinci Crime Family boss Frank Vinci.

### Federal Investigation

After the Assassination of Alberto Clemente, his former Soldato, Henry Tomasino joined with Falcone with the vouching of Falcone Soldato, Vito Scaletta. Carlo Falcone accepted Henry to become part of their organization. It was soon discovered that Henry Tomasino was a rat and was delivering information on Falcone's operations to the Federal Bureau Of Narcotics. This brought a lot of attention on both, Vinci and Falcone factions.

### Carlo Falcone's Death.

After a failed attempt to murder Vinci family Consigliere, Leo Galante, Frank Vinci knew it was only a matter of time before Falcone was going to kill him. Frank Vinci and Leo Galante ordered Soldato Vito Scaletta to kill Carlo Falcone. On September 26th, 1951, Carlo Falcone was murdered by Vito Scaletta with the assistance of fellow Soldato, Joe Barbaro at the Zavesky Observatory in Empire Bay.

Underboss: Eddie Scarpa
Soldato: Vito Scaletta · Joe Barbaro · Antonio Balsamo · Henry Tomasino
Associate: Frankie The Mick · Marty Santorelli
Accountant: Harvey Epstein
Safety

Every member of the lab must complete the course: UC Davis **Laboratory Safety Fundamentals** on the UC Learning website, and go through the site-specific orientation and training for new laboratory personnel.

Please review the following plans, which can also be found in the
brown accordion folder (along with our SOPs and our Emergency Action
Plan) on Paige’s desk:

Molecular and Cellular Biology Department Illness and Injury Prevention Plan: MCB\_IIPP-2

Molecular and Cellular Biology Department Emergency Action Plan: mcb\_eap-2

Laboratory Safety Manual, including Chemical Hygiene Plan: UCD Laboratory Safety Manual-2

Safety Data Sheet Resources: https://ehs.ucop.edu/sds/#/

Specific SDSs for hazardous chemicals in the lab is listed here.
Star Map Room

All of BSCG currently takes place within many different planetary systems located in the Milky Way Galaxy. The Milky Way is a barred spiral galaxy with a diameter of about 100,000 to 180,000 lightyears, and contains around 100 billion planets accompanied by 100-400 billion stars. The star Sol, which lies at the center of the Sol System, is used as the base for measuring distances. To calculate distance from a system to the galactic center (Sagittarius A\*), simply add 26,000 lightyears to the distance from Sol.

## Sol System

* Stars: Sol
* Planets: 8
	+ Mercury
	+ Venus
	+ Earth
	+ Mars
	+ Jupiter(Gas Giant)
	+ Saturn(Gas Giant)
	+ Uranus(Gas Giant)
	+ Neptune(Gas Giant)

The Sol System is where human life originated and is the center of most operations carried out by Nations. Many Nations decided to do a Mass Exodus and leave the Sol System for various reasons such as outgrowing their current space to needing more resources and even wanting to be secluded. Many Nations still reside here however.

## Epsilon Octanus System

* Distance from Sol: 10 lightyears
* Stars: Epsilon Octanus(Main Star), Grath(Miniature Star)
* Planets: 3
	+ Tenelapis
	+ Neo-Earth
	+ Caelmare

## Judaean System

* Distance from Sol: 1,400 lightyears
* Stars: Judaea
* Planets: 7
	+ Makpah
	+ Israel
	+ Ahtede
	+ Tzonen
	+ Sehlah
	+ Keytor (Gas Giant)
	+ Aad (Gas Giant)

The Judaean System was claimed by the Israelis after their exodus from the Sol System. It consists of 7 planets and multiple dwarf planets and asteroids that orbit the star Judaea in the center.

## Shakuran System

* Distance from Sol: 40,000 lightyears
* Stars: Shakar
* Planets: 7
	+ Shi
	+ Braken
	+ Shakuras
	+ Ignus
	+ Xil (Gas Giant)
	+ Nelyth (Gas Giant)
	+ Ynoth (Gas Giant)

The Shakuran System was claimed by the Shakurans after the destruction of their own Vesta System.

## Telos System

* Distance from Sol: 200 lightyears
* Stars: Telos
* Planets: 6
	+ Telos I
	+ Telos II
	+ Telos III
	+ Telos IV
		- Fondor (Asteroid)
	+ Telos V (Gas Giant)
	+ Telos VI (Gas Giant)

The Telos System was claimed by the Albionians after they left the Sol System.
Ritchie Doucet

**Ritchie Doucet** is a character in Mafia III.

## History

Ritchie Doucet is the boss of the Dixie Mafia, a criminal organization allied with the Marcano Crime Family, operating in the Delray Hollow district of New Bordeaux. After the heist of the Federal Reserve money which the Marcano mobsters performed with the help of the Black Mob, Doucet arrives with Sal and Giorgi Marcano at Sammy's Bar in order to get Sal Marcanos Cut. However, Giorgi grabs a Masterson Pistol and shoots Lincoln Clay in the forehead, who unknown to them, survived by an inch.

Doucet then pulls out a knife and brutally stabs Ellis Robinson to death while Giorgi kills Danny Burke and Sal personally kills Sammy Robinson. They then burn the place down and with the Black Mob down, the Dixie Mafia is given control of Delray Hollow by Sal and Doucet sets two Rackets, a Prostitution racket headed by Merle Jackson and a Smack racket headed by Charlie Kincaid.
Technology Department

## Kinetic Weapons

Kinetic Weapons, unlike Energy Weapons, use a projectile that is fired at high velocity towards its target. Once it reaches the target, it will either penetrate the target causing internal damage or be destroyed on impact causing external damage.

### Coilguns

A Coilgun is a type of projectile accelerator consisting of one or more coils used as electromagnets in the configuration of a linear motor that accelerate a ferromagnetic or conducting projectile to high velocity.

### Conventional Artillery

A Conventional Gun or Artillery Piece uses a firing pin that is used to ignite the primer of a bullet. This in turn is used to ignite gunpowder which causes rapidly expanding gases that pushes the projectile out of the gun barrel at high velocity.

### Railguns

A Railgun is a device that uses electromagnetic force to launch high velocity projectiles by means of a sliding armature that is accelerated along a pair of conductive rails.

## Energy Weapons

Energy Weapons, unlike Kinetic Weapons, use a beam of energy or particles which is pointed towards a target in the hopes of burning thru it and causing internal damage.

### Lasers

A Laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation.

### Particle Beams

A Particle Beam weapon uses a high energy beam of atomic or subatomic particles to damage the target by disrupting its atomic and/or molecular structure.
Emperor Erishi

<infobox>

```
 <title source="title">
   <default>Emperor Erishi</default>
 </title>
 <image source="image">

```

 </image>
 <group>
 <header>Personal Details</header>

 <label>Also known as</label>

 <label>Status</label>

 <label>Species</label>

 <label>Age</label>

 <label>Gender</label>

 <label>Actor</label>

 </group>
 <group>
 <header>Appearances</header>

 <label>Seasons</label>

 <label>First Seen</label>

 <label>Last seen</label>

 <label>Death episode</label>

 <label>Appeared in</label>

 </group>
 <group>
 <header>Biography</header>

 <label>Occupation</label>

 <label>Date of birth</label>

 <label>Place of origin</label>

 <label>Date of death</label>

 <label>Cause of death</label>

 <label>Family</label>

 </group>
 <group>
 <header>Physical Attributes</header>

 <label>Ethnicity</label>

 <label>Hair</label>

 <label>Eye</label>

 <label>Height</label>

 <label>Weight</label>

 </group>

**Emperor Erishi** is one of the characters in the Dandelion Dynasty book series.
Hollis Dupree

**Hollis Dupree** is a minor character in Mafia III

## History

### Background

Hollis Dupree is a military veteran who served in World War II and the Korean War. He currently resides in Southdowns.

## Crime

In 1968, Hollis Dupree murdered two African-American men, Lamont Harris, aged 24 and Trey McCall, aged 25, when they came to Dupree's home asking for help with a flat tire. Dupree claims that it was done in self defense and he thought they were there to murder him, though one of them was shot in the lower back.

## Trial

Cornelius Holden was the presiding judge over his case until he was killed by Lincoln Clay to weaken Marcano Crime Family capo Lou Marcano.

## Trivia

* He was on Native Son being interviewed by Remy Duvall.
Ken Liu

Ken Liu is the author of the Dandelion Dynasty and other fantasy book series.

## Biography

Ken Liu is an author and translator of speculative fiction, as well as a lawyer and programmer. He is the winner of the Nebula, Hugo, and World Fantasy awards. He has also been published in The Magazine of Fantasy & Science Fiction, Asimov’s, Analog, Clarkesworld, Lightspeed, and Strange Horizons, among other places. He currently lives with his family near Boston, Massachusetts.
Agarose Gel

1. Weigh enough agarose\* to make a 1% gel (eg 1g/100mL). **\*Make sure the agarose used is specifically for making gels, otherwise your gel will start to dissolve when run at 120V.**
2. Add 1X TAE buffer to the agar in a flask.**\*\*Use a flask large enough that the amount of agar you are making fills it about halfway (for 500mL agarose, use a 1L flask). This will help to ensure the agarose doesn’t overflow when heated.**
3. Swirl and heat in microwave to dissolve. (3-4 mins for 500mL). Watch to make sure it doesn’t boil over in the microwave, and periodically take out and CAREFULLY swirl around to ensure all agarose dissolves (you can check by holding up to the light and looking for the disappearance of whitish/translucent chunks). When it is completely dissolved the liquid should be totally transparent.

Then follow the Gel Electrophoresis steps for casting and running your gel.
Mafia III Portal

**Factions:**

* Marcano Crime Family

* Black Mob
* Italian Gang
* Dixie Mafia
* Southern Union
* Haitian Mob
* Irish Mob
* Lincoln Clay Crime Family

**Characters:**

* Lincoln Clay
* Vito Scaletta
* Cassandra
* Thomas Burke
* Danny Burke
* Nicki Burke
* Sammy Robinson
* Ellis Robinson
* Sal Marcano
* Lou Marcano
* Giorgi Marcano
* Tommy Marcano
* Olivia Marcano
* Remy Duvall
* John Donovan

**New Bordeaux**

* French Ward
* Delray Hollow
* Tickfaw Harbor
* River Row
* Pointe Verdun
* Bayou Fantom
* Downtown
* Southdowns
* Frisco Fields
Growing Bacterial Colonies in LB

In order to grow bacterial colonies (aka "overnight" clones):

1. Obtain 15mL tubes meant for shaking overnight, if you're not sure which ones they are you can ask someone. Label each tube with the construct (ape file info), clone number, date and cell type (XL10, BL21 etc.).
2. Get the correct antibiotic (for your construct) from the -30C, defrost in the 37-degree water bath and keep on ice.
3. Ethanol your benchtop and once it has dried, start a flame with the bunsen burner to keep your area sterile. **\*NOTE: NEVER leave your bench while your flame is on!!! If you need to grab something, close all of your caps and/or plates and turn off your flame.**
4. Using a pipette man, add 5mL of LB media to each tube.
5. Next, add 1x your antibiotic, so 1000x Kanamycin would be 5uL in 5mL of LB. Make sure to add it directly into the LB media (don't release the small volume while your tip is in the air).
6. Then, using a 20uL pipette (set the volume to 10uL or less so bacteria does not get on your pipette) with a new pipette tip for each colony, pick each colony and place it in its respective tube.
7. Once you have all of the tubes ready, place in the 37-degree shaker overnight **(only for 16-20 hours).**
All crime families and gangs

The Commission.

1931-Present.

All Members of The Commission.

Current Year: 2020.

Corleone Crime Family. 1720-Present. Angelo Corleone Jr. [1989-Present].

Gambetti Crime Family. 1920-2017. Roimondo "Roy" Zito. [2008-2017]. (worked under Corleone Family since 2017).

Clemente Crime Family. 1929-1951|1952-2008. Don: Anastasio " Stevie" Gallo.

Gambino Crime Family. 1910-2020. Don: Domenico "Italian Dom" Cefalù. Acting Don Lorenzo Mannino.

Barzini Crime Family. 1934-2025. Don: Paulino "Paulie" Fortunato. [2006-2025].

Pavano Crime Family. 1930-2017. Don: Maria "Mary" Valvona.

Falcone Crime Family. 1933-1951|1954-2008. Don: Rosario Mancuso.

Genovese Crime Family. 1890-2020. Don: Liberio Bellomo.

Tattaglia Crime Family. 1928-2025. Don: Ricardo "Rico" Tattaglia.

Messina Crime Family. 19??-2017. Don: Harvey Noto.

Vinci Crime Family. 1908-2008. Don: Ricciardo "Richie" Carneglia.

Bonanno Crime Family. 1890-2020. Don: Michael "the Nose" Mancuso.

Stracci Crime Family. 19??-2025. Don:

Lupisella Crime Family. 19??-2017. Don: Mark "Loopy" Lupisella. [2008-2017].

Gravina Crime Family. 1932-2008. Don: Tobia Croce. [1993-2008].

Lucchese Crime Family. 19??-2020. Don: Acting Don Michael "Big Mike" DeSantis. [2017-2020].

Cuneo Crime Family. 192?-2025. Don: Ottilio "Leo" Cuneo. [2001-2025[.

Ancelotti Crime Family. 192?-2017. Don: Francesco Trevisano "Frank Trey Angel" Ancelotti. [2009-2017].

Monaco Crime Family. 1968-2020. Don: Benito Monaco. [1988-2020].

Colombo Crime Family. 1928-2020. Don: Acting Don Benjamin "The Claw" Castellazzo.
Prince Phyro

<infobox>

```
 <title source="title">
   <default>Prince Phyro</default>
 </title>
 <image source="image">

```

 </image>
 <group>
 <header>Personal Details</header>

 <label>Also known as</label>

 <label>Status</label>

 <label>Species</label>

 <label>Age</label>

 <label>Gender</label>

 <label>Actor</label>

 </group>
 <group>
 <header>Appearances</header>

 <label>Seasons</label>

 <label>First Seen</label>

 <label>Last seen</label>

 <label>Death episode</label>

 <label>Appeared in</label>

 </group>
 <group>
 <header>Biography</header>

 <label>Occupation</label>

 <label>Date of birth</label>

 <label>Place of origin</label>

 <label>Date of death</label>

 <label>Cause of death</label>

 <label>Family</label>

 </group>
 <group>
 <header>Physical Attributes</header>

 <label>Ethnicity</label>

 <label>Hair</label>

 <label>Eye</label>

 <label>Height</label>

 <label>Weight</label>

 </group>

**Prince Phyro** is one of the main protagonists in the Dandelion Dynasty book series.
Vito Scaletta (Mafia II)

Leo's Prison Gang

**Vito Scaletta** is the Protagonist in Mafia II. He is an upcoming Enforcer in Empire Bay who becomes a Made Man in the Falcone Crime Family.

"*When I first started out I dreamed of Money, Cars, Women, Respect & Freedom.*"
— **Vito Scaletta**

## History

### Background

Vito Scaletta was born to his parents Maria and Antonio Scaletta in Sicily, May 1925. When Vito was 7 years old Antonio Scaletta decided to move to Empire Bay in America because of poverty. Sadly even in Empire Bay they still couldn't escape poverty. He watched his father work himself into death and soon decided the life under the law wasn't for him.

Eventually going to school and growing up on the streets, Vito crossed paths with Joe Barbaro, a bully who eventually became best friends. Vito and Joe were the brains behind a hundred petty crimes, all the while watching all the neighborhoods gangsters and dreaming of the life. Now an adult Vito will risk anything to be somebody.

### Military Enrollment

after a bungled robbery of a jewelry store in 1943, Vito was given a choice, serve the army or go to jail. So Vito enlisted himself to serve as part of the U.S Army in the 504th parachute infantry. On July 11th, 1943, Vito and his squadron were on the way to Southern Sicily but were hit by flak and had to bail out. So when they saw that Mussolini's men were rounding civilians up... They decided to return the favor. In the midst of the battle in San Castele, Sicily a tank arrived and blew up the building they were fighting in, luckily Don Calo, head of the Sicilian Mafia arrived and discussed with the Sicilian Militia to help take Mussolini down, Saving Vito's life.

### Arriving Back To Empire Bay

After being attacked and wounded in Germany 1943, Vito is returning back to Empire Bay on leave. Whilst Joe asks Giuseppe Palminteri to create forged discharged papers for Vito to unenroll from the U.S Army. Once home, Vito discovers that his father, Antonio Scaletta is in debt to a loanshark by $2,000 U.S Dollars. In need of money he talks to Joe and gets a couple of jobs. Working for junkyard dealer, Mike Bruski and Dock Union boss and Vinci Family caporegieme Derek Papalardo.
Giuseppe Russomanno Jr.

Giuseppe Colombano Antonino Russomanno (March 21, 1920 - August 10, 1985) was an Italian-American mob boss based in New Bordeaux. He was first the Don of the Chicago Outfit before his resignation in 1968, to be elected Don of the Marcano crime family, due to his reputation with Salvatore Marcano. His criminal career started in 1933, at the age of 13, stealing jewelry for the Chicago Outfit. He was friends with Tony Spilotro and Frank Cullotta.

## Biography

### Early Life

Giuseppe Colombano Antonino Russomanno Jr. was born on March 21, 1920, in Chicago, Illinois to Giuseppe Russomanno Sr. and Filomena Cicerone. He was raised alongside six brothers, four half-brothers and three half-sisters. Raised with thirteen siblings was rough. He was often ignored or abused by his father. He was pulled into organized crime at an early age, starting his career stealing at the age of 13. Giuseppe was nicknamed "Gyp Junior" by the time he was 16, he was already imprisoned for the murder of 6 people. He was sentenced 25-to-Life imprisonment. Luckily, due to his reputation with the prominent Chicago Outfit, Alphonse Capone, widely known as "Al Capone," made a deal with Judge Browning and only served 5 years.

In 1933, his criminal career began with stealing. He stole for influential mafiosi. On April 1, 1934, he and his father robbed one of the most paramount jewelry stores in Chicago - The Toscano Jewelry. The heist barely succeeded, as they were almost caught. Russomanno was injured during the escape, his leg was caught in a bear trap. Russomanno's father assisted him and they left untouched.