Isoelectric point

1: Zuccaro L, Krieg J, Desideri A, Kern K, Balasubramanian K. Tuning the

isoelectric point of graphene by electrochemical functionalization. Sci Rep. 2015

Jul 2;5:11794. doi: 10.1038/srep11794. PubMed PMID: 26134956; PubMed Central

PMCID: PMC4488746.

2: Feng JL, Qi JR, Yin SW, Wang JM, Guo J, Weng JY, Liu QR, Yang XQ. Fabrication

and Characterization of Stable Soy β-Conglycinin-Dextran Core-Shell Nanogels

Prepared via a Self-Assembly Approach at the Isoelectric Point. J Agric Food

Chem. 2015 Jul 8;63(26):6075-83. doi: 10.1021/acs.jafc.5b01778. Epub 2015 Jun 23.

PubMed PMID: 26075494.

3: Kawate T, Iwaya K, Koshikawa K, Moriya T, Yamasaki T, Hasegawa S, Kaise H,

Fujita T, Matsuo H, Nakamura T, Ishikawa T, Hiroi S, Iguchi-Ariga SM, Ariga H,

Murota K, Fujimori M, Yamamoto J, Matsubara O, Kohno N. High levels of DJ-1

protein and isoelectric point 6.3 isoform in sera of breast cancer patients.

Cancer Sci. 2015 Jul;106(7):938-43. doi: 10.1111/cas.12673. Epub 2015 May 6.

PubMed PMID: 25867058.

4: Kirkwood J, Hargreaves D, O'Keefe S, Wilson J. Using isoelectric point to

determine the pH for initial protein crystallization trials. Bioinformatics. 2015

May 1;31(9):1444-51. doi: 10.1093/bioinformatics/btv011. Epub 2015 Jan 7. PubMed

PMID: 25573921; PubMed Central PMCID: PMC4410668.

5: Dika C, Duval JF, Francius G, Perrin A, Gantzer C. Isoelectric point is an

inadequate descriptor of MS2, Phi X 174 and PRD1 phages adhesion on abiotic

surfaces. J Colloid Interface Sci. 2015 May 15;446:327-34. doi:

10.1016/j.jcis.2014.08.055. Epub 2014 Sep 6. PubMed PMID: 25265875.

6: Bunkute E, Cummins C, Crofts FJ, Bunce G, Nabney IT, Flower DR. PIP-DB: the

Protein Isoelectric Point database. Bioinformatics. 2015 Jan 15;31(2):295-6. doi:

10.1093/bioinformatics/btu637. Epub 2014 Sep 23. PubMed PMID: 25252779.

7: Herzog C, Beckert E, Nagl S. Rapid isoelectric point determination in a

miniaturized preparative separation using jet-dispensed optical pH sensors and

micro free-flow electrophoresis. Anal Chem. 2014 Oct 7;86(19):9533-9. doi:

10.1021/ac501783r. Epub 2014 Sep 23. PubMed PMID: 25211476.

8: Koetting MC, Peppas NA. pH-Responsive poly(itaconic

acid-co-N-vinylpyrrolidone) hydrogels with reduced ionic strength loading

solutions offer improved oral delivery potential for high isoelectric

point-exhibiting therapeutic proteins. Int J Pharm. 2014 Aug 25;471(1-2):83-91.

doi: 10.1016/j.ijpharm.2014.05.023. Epub 2014 May 20. PubMed PMID: 24853463;

PubMed Central PMCID: PMC4096305.

9: Badia-Villanueva M, Carulla P, Carrascal M, Abián J, Llobera M, Casanovas A,

Dolores López-Tejero M. Lipoprotein lipase isoelectric point isoforms in humans.

Biochem Biophys Res Commun. 2014 Mar 7;445(2):480-5. doi:

10.1016/j.bbrc.2014.02.028. Epub 2014 Feb 13. PubMed PMID: 24530399.

10: van der Maaden K, Tomar J, Jiskoot W, Bouwstra J. Chemical modifications of

silicon surfaces for the generation of a tunable surface isoelectric point.

Langmuir. 2014 Feb 25;30(7):1812-9. doi: 10.1021/la404654t. Epub 2014 Feb 11.

PubMed PMID: 24472026.

11: Cornacchia L, Forquenot de la Fortelle C, Venema P. Heat-induced aggregation

of whey proteins in aqueous solutions below their isoelectric point. J Agric Food

Chem. 2014 Jan 22;62(3):733-41. doi: 10.1021/jf404456q. Epub 2014 Jan 8. PubMed

PMID: 24364622.

12: Shimizu T, Mori T, Tomita M, Tsumoto K. pH switching that crosses over the

isoelectric point (pI) can improve the entrapment of proteins within giant

liposomes by enhancing protein-membrane interaction. Langmuir. 2014 Jan

21;30(2):554-63. doi: 10.1021/la403361j. Epub 2014 Jan 6. PubMed PMID: 24359529.

13: Pecini EM, Avena MJ. Measuring the isoelectric point of the edges of clay

mineral particles: the case of montmorillonite. Langmuir. 2013 Dec

3;29(48):14926-34. doi: 10.1021/la403384g. Epub 2013 Nov 21. PubMed PMID:

14: Borghi F, Vyas V, Podestà A, Milani P. Nanoscale roughness and morphology

affect the IsoElectric Point of titania surfaces. PLoS One. 2013 Jul

16;8(7):e68655. doi: 10.1371/journal.pone.0068655. Print 2013. PubMed PMID:

23874708; PubMed Central PMCID: PMC3712945.

15: Thomassen YE, van Eikenhorst G, van der Pol LA, Bakker WA. Isoelectric point

determination of live polioviruses by capillary isoelectric focusing with whole

column imaging detection. Anal Chem. 2013 Jun 18;85(12):6089-94. doi:

10.1021/ac400968q. Epub 2013 May 28. PubMed PMID: 23672432.

16: Hughes AJ, Tentori AM, Herr AE. Bistable isoelectric point photoswitching in

green fluorescent proteins observed by dynamic immunoprobed isoelectric focusing.

J Am Chem Soc. 2012 Oct 24;134(42):17582-91. doi: 10.1021/ja3064292. Epub 2012

Oct 10. PubMed PMID: 23017083; PubMed Central PMCID: PMC3488114.

17: Pihlasalo S, Auranen L, Hänninen P, Härmä H. Method for estimation of protein

isoelectric point. Anal Chem. 2012 Oct 2;84(19):8253-8. doi: 10.1021/ac301569b.

Epub 2012 Sep 18. PubMed PMID: 22946671.

18: Elevi Bardavid R, Oren A. Acid-shifted isoelectric point profiles of the

proteins in a hypersaline microbial mat: an adaptation to life at high salt

concentrations? Extremophiles. 2012 Sep;16(5):787-92. doi:

10.1007/s00792-012-0476-6. Epub 2012 Aug 21. PubMed PMID: 22907126.

19: Freire TS, Clark MW, Comarmond MJ, Payne TE, Reichelt-Brushett AJ, Thorogood

GJ. Electroacoustic isoelectric point determinations of bauxite refinery

residues: different neutralization techniques and minor mineral effects.

Langmuir. 2012 Aug 14;28(32):11802-11. doi: 10.1021/la301790v. Epub 2012 Jul 31.

PubMed PMID: 22783818.

20: Chingin K, Astorga-Wells J, Pirmoradian Najafabadi M, Lavold T, Zubarev RA.

Separation of polypeptides by isoelectric point focusing in electrospray-friendly

solution using a multiple-junction capillary fractionator. Anal Chem. 2012 Aug

7;84(15):6856-62. doi: 10.1021/ac3013016. Epub 2012 Jul 23. PubMed PMID:

21: Okumura H, Fukushima H, Momoda M, Ima Y, Matsuda T, Ujita M. Diverse

lectin-binding specificity of four ZP3 glycoprotein isoforms with a discrete

isoelectric point in chicken egg coat. Biochem Biophys Res Commun. 2012 Aug

3;424(3):586-92. doi: 10.1016/j.bbrc.2012.06.157. Epub 2012 Jul 7. PubMed PMID:

22: Gao HL, Li CY, Ma FX, Wang K, Xu JJ, Chen HY, Xia XH. A nanochannel array

based device for determination of the isoelectric point of confined proteins.

Phys Chem Chem Phys. 2012 Jul 14;14(26):9460-7. doi: 10.1039/c2cp40594f. Epub

2012 May 31. PubMed PMID: 22652811.

23: Kallay N, Preočanin T, Supljika F, Lützenkirchen J, Lovković M. Influence of

interfacial water layer on surface properties of silver halides: effect of pH on

the isoelectric point. J Colloid Interface Sci. 2012 Jun 1;375(1):167-71. doi:

10.1016/j.jcis.2012.02.041. Epub 2012 Mar 6. PubMed PMID: 22443966.

24: Perez-Riverol Y, Audain E, Millan A, Ramos Y, Sanchez A, Vizcaíno JA, Wang R,

Müller M, Machado YJ, Betancourt LH, González LJ, Padrón G, Besada V. Isoelectric

point optimization using peptide descriptors and support vector machines. J

Proteomics. 2012 Apr 3;75(7):2269-74. doi: 10.1016/j.jprot.2012.01.029. Epub 2012

Feb 3. PubMed PMID: 22326964.

25: Kristen-Hochrein N, Laschewsky A, Miller R, von Klitzing R. Stability of foam

films of oppositely charged polyelectrolyte/surfactant mixtures: effect of

isoelectric point. J Phys Chem B. 2011 Dec 15;115(49):14475-83. doi:

10.1021/jp206964k. Epub 2011 Nov 16. PubMed PMID: 22040084.

26: Lecoeur M, Goossens JF, Vaccher C, Bonte JP, Foulon C. A multivariate

approach for the determination of isoelectric point of human carbonic anhydrase

isoforms by capillary isoelectric focusing. Electrophoresis. 2011

Oct;32(20):2857-66. doi: 10.1002/elps.201100219. Epub 2011 Oct 13. PubMed PMID:

27: Khaldi N, Shields DC. Shift in the isoelectric-point of milk proteins as a

consequence of adaptive divergence between the milks of mammalian species. Biol

Direct. 2011 Jul 29;6:40. doi: 10.1186/1745-6150-6-40. PubMed PMID: 21801421;

PubMed Central PMCID: PMC3189186.

28: Dave S, Mahajan S, Chandra V, Gupta P. Trifluoroethanol stabilizes the molten

globule state and induces non-amyloidic turbidity in stem bromelain near its

isoelectric point. Int J Biol Macromol. 2011 Nov 1;49(4):536-42. doi:

10.1016/j.ijbiomac.2011.06.006. Epub 2011 Jun 28. PubMed PMID: 21736896.

29: Maldonado AA, Ribeiro JM, Sillero A. Isoelectric point, electric charge, and

nomenclature of the acid-base residues of proteins. Biochem Mol Biol Educ. 2010

Jul;38(4):230-7. doi: 10.1002/bmb.20405. PubMed PMID: 21567833.

30: Tomita S, Yoshikawa H, Shiraki K. Arginine controls heat-induced

cluster-cluster aggregation of lysozyme at around the isoelectric point.

Biopolymers. 2011 Oct;95(10):695-701. doi: 10.1002/bip.21637. Epub 2011 Apr 20.

PubMed PMID: 21509744.

31: Alendé N, Nielsen JE, Shields DC, Khaldi N. Evolution of the isoelectric

point of mammalian proteins as a consequence of indels and adaptive evolution.

Proteins. 2011 May;79(5):1635-48. doi: 10.1002/prot.22990. Epub 2011 Mar 8.

PubMed PMID: 21387414.

32: Muto T, Taniguchi H, Kushima R, Tsuda H, Yonemori H, Chen C, Sugihara Y,

Sakamoto K, Kobori Y, Palmer H, Nakamura Y, Tomonaga T, Tanaka H, Mizushima H,

Fujita S, Kondo T. Global expression study in colorectal cancer on proteins with

alkaline isoelectric point by two-dimensional difference gel electrophoresis. J

Proteomics. 2011 May 16;74(6):858-73. doi: 10.1016/j.jprot.2011.02.030. Epub 2011

Mar 6. PubMed PMID: 21385629.

33: Mazzoli R, Fattori P, Lamberti C, Giuffrida MG, Zapponi M, Giunta C, Pessione

E. High isoelectric point sub-proteome analysis of Acinetobacter radioresistens

S13 reveals envelope stress responses induced by aromatic compounds. Mol Biosyst.

2011 Mar;7(3):598-607. doi: 10.1039/c0mb00112k. Epub 2010 Oct 18. PubMed PMID:

34: Cologna SM, Russell WK, Lim PJ, Vigh G, Russell DH. Combining isoelectric

point-based fractionation, liquid chromatography and mass spectrometry to improve

peptide detection and protein identification. J Am Soc Mass Spectrom. 2010

Sep;21(9):1612-9. doi: 10.1016/j.jasms.2010.04.010. Epub 2010 Apr 24. PubMed

PMID: 20537905; PubMed Central PMCID: PMC2927729.

35: Jeon WB. Retrospective analyses of the bottleneck in purification of

eukaryotic proteins from Escherichia coli as affected by molecular weight,

cysteine content and isoelectric point. BMB Rep. 2010 May;43(5):319-24. PubMed

PMID: 20510014.

36: Santiago PS, Carvalho FA, Domingues MM, Carvalho JW, Santos NC, Tabak M.

Isoelectric point determination for Glossoscolex paulistus extracellular

hemoglobin: oligomeric stability in acidic pH and relevance to protein-surfactant

interactions. Langmuir. 2010 Jun 15;26(12):9794-801. doi: 10.1021/la100060p.

PubMed PMID: 20423061.

37: Müller C, Lüders A, Hoth-Hannig W, Hannig M, Ziegler C. Initial bioadhesion

on dental materials as a function of contact time, pH, surface wettability, and

isoelectric point. Langmuir. 2010 Mar 16;26(6):4136-41. doi: 10.1021/la903299y.

PubMed PMID: 19888741.

38: Kajiya H, Takekoshi S, Takei M, Egashira N, Miyakoshi T, Serizawa A, Teramoto

A, Osamura RY. Selection of buffer pH by the isoelectric point of the antigen for

the efficient heat-induced epitope retrieval: re-appraisal for nuclear protein

pathobiology. Histochem Cell Biol. 2009 Dec;132(6):659-67. doi:

10.1007/s00418-009-0635-8. Epub 2009 Sep 19. PubMed PMID: 19768463.

39: Ma H, Forssell P, Partanen R, Seppänen R, Buchert J, Boer H. Sodium

caseinates with an altered isoelectric point as emulsifiers in oil/water systems.

J Agric Food Chem. 2009 May 13;57(9):3800-7. doi: 10.1021/jf803104s. PubMed PMID:

40: Srivastava V, Srivastava MK, Chibani K, Nilsson R, Rouhier N, Melzer M,

Wingsle G. Alternative splicing studies of the reactive oxygen species gene

network in Populus reveal two isoforms of high-isoelectric-point superoxide

dismutase. Plant Physiol. 2009 Apr;149(4):1848-59. doi: 10.1104/pp.108.133371.

Epub 2009 Jan 28. PubMed PMID: 19176719; PubMed Central PMCID: PMC2663752.

41: Hilal SH, Karickhoff SW, Carreira LA. Estimation of microscopic, zwitterionic

ionization constants, isoelectric point and molecular speciation of organic

compounds. Talanta. 1999 Nov 15;50(4):827-40. PubMed PMID: 18967773.

42: Schuurmans Stekhoven FM, Gorissen MH, Flik G. The isoelectric point, a key to

understanding a variety of biochemical problems: a minireview. Fish Physiol

Biochem. 2008 Mar;34(1):1-8. doi: 10.1007/s10695-007-9145-6. Epub 2007 Aug 9.

Review. PubMed PMID: 18649017.

43: Cargile BJ, Sevinsky JR, Essader AS, Eu JP, Stephenson JL Jr. Calculation of

the isoelectric point of tryptic peptides in the pH 3.5-4.5 range based on

adjacent amino acid effects. Electrophoresis. 2008 Jul;29(13):2768-78. doi:

10.1002/elps.200700701. PubMed PMID: 18615785.

44: Bordi F, Cametti C, Sennato S, Truzzolillo D. Strong repulsive interactions

in polyelectrolyte-liposome clusters close to the isoelectric point: a sign of an

arrested state. Phys Rev E Stat Nonlin Soft Matter Phys. 2007 Dec;76(6 Pt

1):061403. Epub 2007 Dec 26. PubMed PMID: 18233846.

45: Maldonado A, Vara F, Sillero A. Improved application of the oscillating

method for the isoelectric point determination of protein: potential connection

with protein data banks. Comput Biol Med. 2008 Jan;38(1):23-30. Epub 2007 Jul 25.

PubMed PMID: 17655838.

46: Kusov Y, Gauss-Müller V, Morace G. Immunogenic epitopes on the surface of the

hepatitis A virus capsid: Impact of secondary structure and/or isoelectric point

on chimeric virus assembly. Virus Res. 2007 Dec;130(1-2):296-302. Epub 2007 Jul

20. PubMed PMID: 17640757.

47: Kiraga J, Mackiewicz P, Mackiewicz D, Kowalczuk M, Biecek P, Polak N,

Smolarczyk K, Dudek MR, Cebrat S. The relationships between the isoelectric point

and: length of proteins, taxonomy and ecology of organisms. BMC Genomics. 2007

Jun 12;8:163. PubMed PMID: 17565672; PubMed Central PMCID: PMC1905920.

48: Healy TW, Fuerstenau DW. The isoelectric point/point-of zero-charge of

interfaces formed by aqueous solutions and nonpolar solids, liquids, and gases. J

Colloid Interface Sci. 2007 May 1;309(1):183-8. Epub 2007 Jan 25. PubMed PMID:

49: Srivastava V, Schinkel H, Witzell J, Hertzberg M, Torp M, Srivastava MK,

Karpinska B, Melzer M, Wingsle G. Downregulation of high-isoelectric-point

extracellular superoxide dismutase mediates alterations in the metabolism of

reactive oxygen species and developmental disturbances in hybrid aspen. Plant J.

2007 Jan;49(1):135-48. PubMed PMID: 17233796.

50: Bromley EH, Krebs MR, Donald AM. Mechanisms of structure formation in

particulate gels of beta-lactoglobulin formed near the isoelectric point. Eur

Phys J E Soft Matter. 2006 Oct;21(2):145-52. Epub 2006 Dec 7. PubMed PMID:

51: Peronnet E, Becquart L, Martinez J, Charrier JP, Jolivet-Reynaud C.

Isoelectric point determination of cardiac troponin I forms present in plasma

from patients with myocardial infarction. Clin Chim Acta. 2007

Feb;377(1-2):243-7. Epub 2006 Oct 13. PubMed PMID: 17113062.

52: Bullard JW, Cima MJ. Orientation dependence of the isoelectric point of TiO2

(rutile) surfaces. Langmuir. 2006 Nov 21;22(24):10264-71. PubMed PMID: 17107031.

53: Aslan FM, Yu Y, Vajda S, Mohr SC, Cantor CR. Engineering a novel, stable

dimeric streptavidin with lower isoelectric point. J Biotechnol. 2007 Feb

1;128(2):213-25. Epub 2006 Sep 26. PubMed PMID: 17000019.

54: Wu J, Huang T. Peak identification in capillary isoelectric focusing using

the concept of relative peak position as determined by two isoelectric point

markers. Electrophoresis. 2006 Sep;27(18):3584-90. PubMed PMID: 16927345.

55: Assemi S, Nalaskowski J, Miller JD, Johnson WP. Isoelectric point of fluorite

by direct force measurements using atomic force microscopy. Langmuir. 2006 Feb

14;22(4):1403-5. PubMed PMID: 16460053.

56: Valnickova Z, Christensen T, Skottrup P, Thøgersen IB, Højrup P, Enghild JJ.

Post-translational modifications of human thrombin-activatable fibrinolysis

inhibitor (TAFI): evidence for a large shift in the isoelectric point and reduced

solubility upon activation. Biochemistry. 2006 Feb 7;45(5):1525-35. PubMed PMID:

57: Sillero A, Maldonado A. Isoelectric point determination of proteins and other

macromolecules: oscillating method. Comput Biol Med. 2006 Feb;36(2):157-66. Epub

2005 Jan 1. PubMed PMID: 16389075.

58: Sahab ZJ, Suh Y, Sang QX. Isoelectric point-based prefractionation of

proteins from crude biological samples prior to two-dimensional gel

electrophoresis. J Proteome Res. 2005 Nov-Dec;4(6):2266-72. PubMed PMID:

59: Franks GV. Zeta potentials and yield stresses of silica suspensions in

concentrated monovalent electrolytes: isoelectric point shift and additional

attraction. J Colloid Interface Sci. 2002 May 1;249(1):44-51. PubMed PMID:

60: Rodríguez-Piñeiro AM, Carvajal-Rodríguez A, Rolán-Alvarez E,

Rodríguez-Berrocal FJ, Martínez-Fernández M, Páez de la Cadena M. Application of

relative warp analysis to the evaluation of two-dimensional gels in proteomics:

studying isoelectric point and relative molecular mass variation. J Proteome Res.

2005 Jul-Aug;4(4):1318-23. PubMed PMID: 16083282.

61: Rezwan K, Meier LP, Gauckler LJ. A prediction method for the isoelectric

point of binary protein mixtures of bovine serum albumin and lysozyme adsorbed on

colloidal titania and alumina particles. Langmuir. 2005 Apr 12;21(8):3493-7.

PubMed PMID: 15807593.

62: Shi Q, Zhou Y, Sun Y. Influence of pH and ionic strength on the steric

mass-action model parameters around the isoelectric point of protein. Biotechnol

Prog. 2005 Mar-Apr;21(2):516-23. PubMed PMID: 15801792.

63: Stastná M, Trávnícek M, Slais K. New azo dyes as colored isoelectric point

markers for isoelectric focusing in acidic pH region. Electrophoresis. 2005

Jan;26(1):53-9. PubMed PMID: 15624139.

64: Allen L, Weinberger J, Weinberger R. Determination of impurities in the drug

5-aminosalicylic acid by micellar electrokinetic capillary chromatography using

an electrolyte pH that approaches the isoelectric point of the parent compound. J

Chromatogr A. 2004 Oct 22;1053(1-2):217-26. PubMed PMID: 15543986.

65: Kosmulski M, Saneluta C. Point of zero charge/isoelectric point of exotic

oxides: Tl2O3. J Colloid Interface Sci. 2004 Dec 15;280(2):544-5. PubMed PMID:

66: Liu E, Moriyama H, Abiru N, Paronen J, Devendra D, Finkelman FD, Eisenbarth

GS. Preventing peptide-induced anaphylaxis: addition of C-terminal amino acids to

produce a neutral isoelectric point. J Allergy Clin Immunol. 2004

Sep;114(3):607-13. PubMed PMID: 15356565.

67: Huber T, Kobe B. Comment on 'protein isoelectric point as a predictor for

increased crystallization screening efficiency'. Bioinformatics. 2004 Sep

22;20(14):2169-70; author reply 2171-4. Epub 2004 Aug 18. PubMed PMID: 15317693.

68: de Vries R. Monte Carlo simulations of flexible polyanions complexing with

whey proteins at their isoelectric point. J Chem Phys. 2004 Feb

15;120(7):3475-81. PubMed PMID: 15268505.

69: Righetti PG. Determination of the isoelectric point of proteins by capillary

isoelectric focusing. J Chromatogr A. 2004 May 28;1037(1-2):491-9. Review. PubMed

PMID: 15214685.

70: Cargile BJ, Bundy JL, Freeman TW, Stephenson JL Jr. Gel based isoelectric

focusing of peptides and the utility of isoelectric point in protein

identification. J Proteome Res. 2004 Jan-Feb;3(1):112-9. PubMed PMID: 14998171.

71: Zocchi A, Jobé AM, Neuhaus JM, Ward TR. Expression and purification of a

recombinant avidin with a lowered isoelectric point in Pichia pastoris. Protein

Expr Purif. 2003 Dec;32(2):167-74. PubMed PMID: 14965761.

72: Kantardjieff KA, Rupp B. Protein isoelectric point as a predictor for

increased crystallization screening efficiency. Bioinformatics. 2004 Sep

22;20(14):2162-8. Epub 2004 Feb 10. PubMed PMID: 14871873.

73: Andersen T, Pepaj M, Trones R, Lundanes E, Greibrokk T. Isoelectric point

separation of proteins by capillary pH-gradient ion-exchange chromatography. J

Chromatogr A. 2004 Feb 6;1025(2):217-26. PubMed PMID: 14763806.

74: Liu HX, Zhang RS, Yao XJ, Liu MC, Hu ZD, Fan BT. Prediction of the

isoelectric point of an amino acid based on GA-PLS and SVMs. J Chem Inf Comput

Sci. 2004 Jan-Feb;44(1):161-7. PubMed PMID: 14741023.

75: Cargile BJ, Stephenson JL Jr. An alternative to tandem mass spectrometry:

isoelectric point and accurate mass for the identification of peptides. Anal

Chem. 2004 Jan 15;76(2):267-75. PubMed PMID: 14719870.

76: Goodridge L, Goodridge C, Wu J, Griffiths M, Pawliszyn J. Isoelectric point

determination of norovirus virus-like particles by capillary isoelectric focusing

with whole column imaging detection. Anal Chem. 2004 Jan 1;76(1):48-52. PubMed

PMID: 14697031.

77: Kempná P, Cipollone R, Villacorta L, Ricciarelli R, Zingg JM. Isoelectric

point mobility shift assay for rapid screening of charged and uncharged ligands

bound to proteins. IUBMB Life. 2003 Feb;55(2):103-7. PubMed PMID: 12749692.

78: Florio W, Batoni G, Esin S, Bottai D, Maisetta G, Pardini M, Campa M.

Identification of novel proteins in culture filtrates of Mycobacterium bovis

bacillus Calmette-Guérin in the isoelectric point range 6-11. Proteomics. 2003

May;3(5):798-802. PubMed PMID: 12748957.

79: Melkko S, Halin C, Borsi L, Zardi L, Neri D. An antibody-calmodulin fusion

protein reveals a functional dependence between macromolecular isoelectric point

and tumor targeting performance. Int J Radiat Oncol Biol Phys. 2002 Dec

1;54(5):1485-90. PubMed PMID: 12459375.

80: Jin Y, Luo G, Oka T, Manabe T. Estimation of isoelectric points of human

plasma proteins employing capillary isoelectric focusing and peptide isoelectric

point markers. Electrophoresis. 2002 Sep;23(19):3385-91. PubMed PMID: 12373767.

81: Vitt UA, Nayudu PL, Rose UM, Kloosterboer HJ. Embryonic development after

follicle culture is influenced by follicle-stimulating hormone isoelectric point

range. Biol Reprod. 2001 Nov;65(5):1542-7. PubMed PMID: 11673273.

82: Karpinska B, Karlsson M, Schinkel H, Streller S, Süss KH, Melzer M, Wingsle

G. A novel superoxide dismutase with a high isoelectric point in higher plants.

expression, regulation, and protein localization. Plant Physiol. 2001

Aug;126(4):1668-77. PubMed PMID: 11500564; PubMed Central PMCID: PMC117165.

83: Onda M, Nagata S, Tsutsumi Y, Vincent JJ, Wang Q, Kreitman RJ, Lee B, Pastan

I. Lowering the isoelectric point of the Fv portion of recombinant immunotoxins

leads to decreased nonspecific animal toxicity without affecting antitumor

activity. Cancer Res. 2001 Jul 1;61(13):5070-7. PubMed PMID: 11431343.

84: Lin FY, Chen CS, Chen WY, Yamamoto S. Microcalorimetric studies of the

interaction mechanisms between proteins and Q-sepharose at pH near the

isoelectric point (pI) effects of NaCl concentration, pH value, and temperature.

J Chromatogr A. 2001 Apr 6;912(2):281-9. PubMed PMID: 11330797.

85: Shimura K, Zhi W, Matsumoto H, Kasai K. Accuracy in the determination of

isoelectric points of some proteins and a peptide by capillary isoelectric

focusing: utility of synthetic peptides as isoelectric point markers. Anal Chem.

2000 Oct 1;72(19):4747-57. PubMed PMID: 11028642.

86: Frandsen TP, Lok F, Mirgorodskaya E, Roepstorff P, Svensson B. Purification,

enzymatic characterization, and nucleotide sequence of a high-isoelectric-point

alpha-glucosidase from barley malt. Plant Physiol. 2000 May;123(1):275-86. PubMed

PMID: 10806244; PubMed Central PMCID: PMC59001.

87: Hempe JM, Craver RD. Separation of hemoglobin variants with similar charge by

capillary isoelectric focusing: value of isoelectric point for identification of

common and uncommon hemoglobin variants. Electrophoresis. 2000 Mar;21(4):743-8.

PubMed PMID: 10733215.

88: Shimura K, Wang Z, Matsumoto H, Kasai K. Synthetic oligopeptides as

isoelectric point markers for capillary isoelectric focusing with ultraviolet

absorption detection. Electrophoresis. 2000 Feb;21(3):603-10. PubMed PMID:

89: Onda M, Kreitman RJ, Vasmatzis G, Lee B, Pastan I. Reduction of the

nonspecific animal toxicity of anti-Tac(Fv)-PE38 by mutations in the framework

regions of the Fv which lower the isoelectric point. J Immunol. 1999 Dec

1;163(11):6072-7. PubMed PMID: 10570296.

90: Richards DP, Stathakis C, Polakowski R, Ahmadzadeh H, Dovichi NJ. Labeling

effects on the isoelectric point of green fluorescent protein. J Chromatogr A.

1999 Aug 20;853(1-2):21-5. PubMed PMID: 10486708.

91: Bendahmane M, Koo M, Karrer E, Beachy RN. Display of epitopes on the surface

of tobacco mosaic virus: impact of charge and isoelectric point of the epitope on

virus-host interactions. J Mol Biol. 1999 Jul 2;290(1):9-20. PubMed PMID:

92: Yao Z, Zhang M, Sakahara H, Nakamoto Y, Higashi T, Zhao S, Sato N, Arano Y,

Konishi J. The relationship of glycosylation and isoelectric point with tumor

accumulation of avidin. J Nucl Med. 1999 Mar;40(3):479-83. PubMed PMID: 10086714.

93: Legendre JM, Bergot A, Turzo A, Morin PP, Humphery-Smith I. [Hemoglobin alpha

chain isoelectric point modification under the action of urea, sodium cyanate,

succinic anhydride or diethylene triamine pentaacetic acid anhydride]. Pathol

Biol (Paris). 1998 Oct;46(8):605-12. French. PubMed PMID: 9871932.

94: Tan PH, Chu V, Stray JE, Hamlin DK, Pettit D, Wilbur DS, Vessella RL, Stayton

PS. Engineering the isoelectric point of a renal cell carcinoma targeting

antibody greatly enhances scFv solubility. Immunotechnology. 1998

Oct;4(2):107-14. PubMed PMID: 9853952.

95: Gotzmann J, Eger A, Meissner M, Grimm R, Gerner C, Sauermann G, Foisner R.

Two-dimensional electrophoresis reveals a nuclear matrix-associated nucleolin

complex of basic isoelectric point. Electrophoresis. 1997 Dec;18(14):2645-53.

PubMed PMID: 9527495.

96: Dowd SE, Pillai SD, Wang S, Corapcioglu MY. Delineating the specific

influence of virus isoelectric point and size on virus adsorption and transport

through sandy soils. Appl Environ Microbiol. 1998 Feb;64(2):405-10. PubMed PMID:

9464373; PubMed Central PMCID: PMC106058.

97: McCafferty E, Wightman JP. Determination of the Surface Isoelectric Point of

Oxide Films on Metals by Contact Angle Titration. J Colloid Interface Sci. 1997

Oct 15;194(2):344-55. PubMed PMID: 9398416.

98: Pagliaccia C, Manetti R, Rappuoli R. Pertactin antigens extracted from

Bordetella pertussis and Bordetella bronchiseptica differ in the isoelectric

point. Arch Microbiol. 1997 Nov;168(5):437-40. PubMed PMID: 9325433.

99: Wilkins MR, Williams KL. Cross-species protein identification using amino

acid composition, peptide mass fingerprinting, isoelectric point and molecular

mass: a theoretical evaluation. J Theor Biol. 1997 May 7;186(1):7-15. PubMed

PMID: 9176634.

100: Petitt P, Edwards ME, Forciniti D. A small-angle neutron scattering study of

gamma-crystallins near their isoelectric point. Eur J Biochem. 1997 Jan

15;243(1-2):415-21. PubMed PMID: 9030767.

101: Gangopadhyay A, Petrick AT, Thomas P. Modification of antibody isoelectric

point affects biodistribution of 111-indium-labeled antibody. Nucl Med Biol. 1996

Apr;23(3):257-61. PubMed PMID: 8782234.

102: Chen N, Chrambach A. Enhanced field strength and resolution in gel

electrophoresis upon substitution of buffer by histidine at its isoelectric

point. Electrophoresis. 1996 Apr;17(4):699-703. PubMed PMID: 8738330.

103: Woitas RP, Morioka T. Influence of isoelectric point on glomerular

deposition of antibodies and immune complexes. Nephron. 1996;74(4):713-9. PubMed

PMID: 8956307.

104: Sergeant N, Bussière T, Vermersch P, Lejeune JP, Delacourte A. Isoelectric

point differentiates PHF-tau from biopsy-derived human brain tau proteins.

Neuroreport. 1995 Nov 13;6(16):2217-20. PubMed PMID: 8595206.

105: Patrickios CS, Yamasaki EN. Polypeptide amino acid composition and

isoelectric point. II. Comparison between experiment and theory. Anal Biochem.

1995 Oct 10;231(1):82-91. PubMed PMID: 8678324.

106: Shimura K, Kasai K. Fluorescence-labeled peptides as isoelectric point (pI)

markers in capillary isoelectric focusing with fluorescence detection.

Electrophoresis. 1995 Aug;16(8):1479-84. PubMed PMID: 8529618.

107: Zhu YC, Harn DA, Quinn JJ. Characterization of isoelectric point (pI)

fractions of soluble egg antigen of Schistosoma japonicum. Zhongguo Ji Sheng

Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(1):13-9. PubMed PMID: 8044897.

108: Walter H, Forciniti D. Cross-partitioning: determination of isoelectric

point by partitioning. Methods Enzymol. 1994;228:223-33. PubMed PMID: 7519284.

109: Okada M, Harima H, Hosoi Y, Sogo A. Influences of some low molecular

compounds on enzymatic activity and isoelectric point of aspartate

aminotransferase from rat liver. J Nutr Sci Vitaminol (Tokyo). 1993

Aug;39(4):311-21. PubMed PMID: 8283311.

110: Strang CJ, Wales ME, Brown DM, Wild JR. Site-directed alterations to the

geometry of the aspartate transcarbamoylase zinc domain: selective alteration to

regulation by heterotropic ligands, isoelectric point, and stability in urea.

Biochemistry. 1993 Apr 27;32(16):4156-67. PubMed PMID: 8476846.

111: Chardon H, Pachetti C, Collet L, Bellon O, Lagier E. [Determination of the

isoelectric point of beta-lactamases isolated from 67 Klebsiella oxytoca strains

and phenotype behaviour against eight beta-lactam antibiotics]. Pathol Biol

(Paris). 1993 Apr;41(4):343-8. French. PubMed PMID: 8233633.

112: Zahner JE, Cheney CM. A Drosophila homolog of bovine smg p25a GDP

dissociation inhibitor undergoes a shift in isoelectric point in the

developmental mutant quartet. Mol Cell Biol. 1993 Jan;13(1):217-27. PubMed PMID:

8417327; PubMed Central PMCID: PMC358901.

113: Bush E, Currie RA. Determination of transcription factor isoelectric point

by two-dimensional native isoelectric focusing and electrophoretic mobility shift

analysis. Anal Biochem. 1992 Oct;206(1):189-94. PubMed PMID: 1456433.

114: Cerban F, Gruppi A, Vottero-Cima E. Trypanosoma cruzi: involvement of IgG

isotypes in the parasitemia control of mice immunized with parasite exoantigens

of isoelectric point 4.5. Exp Parasitol. 1992 Aug;75(1):137-45. PubMed PMID:

115: Kato T, Yamagata Y, Arai T, Ichishima E. Purification of a new extracellular

90-kDa serine proteinase with isoelectric point of 3.9 from Bacillus subtilis

(natto) and elucidation of its distinct mode of action. Biosci Biotechnol

Biochem. 1992 Jul;56(7):1166-8. PubMed PMID: 1368833.

116: Shinagawa T, Ohshima H, Kondo T. Isoelectric point of an ion-penetrable

membrane. Biophys Chem. 1992 Jun;43(2):149-56. PubMed PMID: 1498249.

117: Pogliani L. Molecular connectivity model for determination of isoelectric

point of amino acids. J Pharm Sci. 1992 Apr;81(4):334-6. PubMed PMID: 1501067.

118: Forciniti D, Hall CK, Kula MR. Protein partitioning at the isoelectric

point: influence of polymer molecular weight and concentration and protein size.

Biotechnol Bioeng. 1991 Nov;38(9):986-94. PubMed PMID: 18600861.

119: Muranova TA, Shvyrkova IG. [Anomalous behavior of certain proteins having an

isoelectric point near 4.5 during their precipitation from aqueous solutions from

organic solvents]. Bioorg Khim. 1991 Aug;17(8):1038-40. Russian. PubMed PMID:

120: Englund AK, Lundahl P. The isoelectric point of the human red cell glucose

transporter. Biochim Biophys Acta. 1991 Jun 18;1065(2):185-94. PubMed PMID:

121: Ribeiro JM, Sillero A. A program to calculate the isoelectric point of

macromolecules. Comput Biol Med. 1991;21(3):131-41. PubMed PMID: 1914443.

122: Slitine FE, Toulmond A. Two-dimensional electrophoresis of Arenicola marina

extracellular hemoglobin: separation of chains with identical molecular mass but

different isoelectric point. Comp Biochem Physiol B. 1991;100(3):631-4. PubMed

PMID: 1814687.

123: Birgens HS, Kristensen LO. Impaired receptor binding and decrease in

isoelectric point of lactoferrin after interaction with human monocytes. Eur J

Haematol. 1990 Jul;45(1):31-5. PubMed PMID: 2165916.

124: ten Kate CI, Fischman AJ, Rubin RH, Fucello AJ, Riexinger D, Wilkinson RA,

Du L, Khaw BA, Strauss HW. Effect of isoelectric point on biodistribution and

inflammation: imaging with indium-111-labelled IgG. Eur J Nucl Med.

1990;17(6-8):305-9. PubMed PMID: 2286203.

125: Bellotti V, Merlini G, Bucciarelli E, Perfetti V, Quaglini S, Ascari E.

Relevance of class, molecular weight and isoelectric point in predicting human

light chain amyloidogenicity. Br J Haematol. 1990 Jan;74(1):65-9. PubMed PMID:

126: Farías RN, López Viñals AE, Posse E, Morero RD. Relationship between

isoelectric point of native and chemically modified insulin and liposomal fusion.

Biochem J. 1989 Nov 15;264(1):285-7. PubMed PMID: 2690824; PubMed Central PMCID:

PMC1133574.

127: Quemeneur E, Marcillat O, Eichenberger D, Vial C. Isoelectric point

heterogeneity of the two oligomeric forms of heart mitochondrial creatine kinase.

Biochem Int. 1989 Feb;18(2):365-71. PubMed PMID: 2764954.

128: Ikeda T, Ubuka T, Ishino K. Determination of the isoelectric point value of

3-mercaptopyruvate sulfurtransferase and its shift by treatment with oxidized

glutathione. Acta Med Okayama. 1989 Feb;43(1):1-7. PubMed PMID: 2718769.

129: Snyder FF, Joyce JE, Carter-Edwards T, Joshi R, Rylance HL, Wallace RC, Nuki

G. Hypoxanthine-guanine phosphoribosyltransferase deficiency in three brothers

with gout: characterization of a variant, HPRTEdinburgh, having altered

isoelectric point, increased thermal lability and normal levels of messenger RNA.

J Inherit Metab Dis. 1989;12(4):390-402. PubMed PMID: 2516172.

130: Norden AG, Flynn FV, Fulcher LM, Richards JD. Renal impairment in myeloma:

negative association with isoelectric point of excreted Bence-Jones protein. J

Clin Pathol. 1989 Jan;42(1):59-62. PubMed PMID: 2466054; PubMed Central PMCID:

PMC1141792.

131: Jacobsen JV, Bush DS, Sticher L, Jones RL. Evidence for precursor forms of

the low isoelectric point alpha-amylase isozymes secreted by barley aleurone

cells. Plant Physiol. 1988 Dec;88(4):1168-74. PubMed PMID: 16666439; PubMed

Central PMCID: PMC1055735.

132: Park KS, Frost BF, Shin S, Park IK, Kim S, Paik WK. Effect of enzymatic

methylation of yeast iso-1-cytochrome c on its isoelectric point. Arch Biochem

Biophys. 1988 Nov 15;267(1):195-204. PubMed PMID: 2848448.

133: Persechini PM, Young JD. The primary structure of the lymphocyte

pore-forming protein perforin: partial amino acid sequencing and determination of

isoelectric point. Biochem Biophys Res Commun. 1988 Oct 31;156(2):740-5. PubMed

PMID: 3190678.

134: Suzuki J, Kobayashi S, Kagaya K, Fukazawa Y. Heterogeneity of hemolytic

efficiency and isoelectric point of streptolysin O. Infect Immun. 1988

Sep;56(9):2474-8. PubMed PMID: 3045002; PubMed Central PMCID: PMC259590.

135: Ubuka T, Masuoka N, Yoshida S, Ishino K. Determination of isoelectric point

value of 3-mercaptopyruvate sulfurtransferase by isoelectric focusing using

ribonuclease A-glutathione mixed disulfides as standards. Anal Biochem. 1987

Dec;167(2):284-9. PubMed PMID: 3481677.

136: Walsh MJ, Shapshak P, Graves MC, Imagawa DT, Tourtellotte WW. Isoelectric

point restriction of cerebrospinal fluid and serum IgG antibodies to measles

virus polypeptides in multiple sclerosis. J Neuroimmunol. 1987 Apr;14(3):243-52.

PubMed PMID: 3549773.

137: Bontrop RE, Baas EJ, Otting N, Schreuder GM, Giphart MJ. Molecular diversity

of HLA-DQ. DQ alpha and beta chain isoelectric point differences and their

relation to serologically defined HLA-DQ allospecificities. Immunogenetics.

1987;25(5):305-12. PubMed PMID: 3570381.

138: Eckersall PD, Thomas A, Marshall GM, Douglas TA. The effect of neuraminidase

on the molecular weight and the isoelectric point of the steroid induced alkaline

phosphatase of dogs. J Comp Pathol. 1986 Sep;96(5):587-91. PubMed PMID: 3760270.

139: Schreuder GM, Tilanus MG, Bontrop RE, Bruining GJ, Giphart MJ, van Rood JJ,

de Vries RR. HLA-DO polymorphism associated with resistance to type I diabetes

detected with monoclonal antibodies, isoelectric point differences, and

restriction fragment length polymorphism. J Exp Med. 1986 Sep 1;164(3):938-43.

PubMed PMID: 3462303; PubMed Central PMCID: PMC2188401.

140: Eng H, Robertson JA, Stemke GW. Properties of urease from Ureaplasma

urealyticum: kinetics, molecular weight, and demonstration of multiple enzyme

isoelectric point forms. Can J Microbiol. 1986 Jun;32(6):487-93. PubMed PMID:

141: Green MS, Hossler PA, Castor CW. Connective tissue activation. XXX:

Isoelectric point microheterogeneity of CTAP-III, a human platelet-derived growth

factor. Proc Soc Exp Biol Med. 1986 Apr;181(4):555-9. PubMed PMID: 3081909.

142: Candiano G, Ghiggeri GM, Delfino G, Queirolo C, Vecchio G. Isoelectric

focusing in low-denaturing media: visualization in renal disease of variation of

the isoelectric point of albumin not related to a remarkable conformational

variation. J Chromatogr. 1986 Jan 24;374(2):279-88. PubMed PMID: 2420813.

143: Palant CE, Bonitati J, Bartholomew WR, Brentjens JR, Walshe JJ, Bentzel CJ.

Nodular glomerulosclerosis associated with multiple myeloma. Role of light chain

isoelectric point. Am J Med. 1986 Jan;80(1):98-102. PubMed PMID: 3079952.

144: Moguilevsky N, Retegui LA, Masson PL. Comparison of human lactoferrins from

milk and neutrophilic leucocytes. Relative molecular mass, isoelectric point,

iron-binding properties and uptake by the liver. Biochem J. 1985 Jul

15;229(2):353-9. PubMed PMID: 4038272; PubMed Central PMCID: PMC1145067.

145: Yang VC, Langer R. pH-dependent binding analysis, a new and rapid method for

isoelectric point estimation. Anal Biochem. 1985 May 15;147(1):148-55. PubMed

PMID: 4025813.

146: Högstorp H, Jacobsson H, Wallin R, Saldeen T. Two forms of alpha

2-antiplasmin: differences in KAV and isoelectric point. Thromb Res. 1984 Sep

1;35(5):559-65. PubMed PMID: 6484898.

147: Melcion C, Mougenot B, Baudouin B, Ronco P, Moulonguet-Doleris L, Vanhille

P, Beaufils M, Morel-Maroger L, Verroust P, Richet G. Renal failure in myeloma:

relationship with isoelectric point of immunoglobulin light chains. Clin Nephrol.

1984 Sep;22(3):138-43. PubMed PMID: 6435919.

148: Iavorkovskiĭ LI, Blumberga IA, Velik LV, Pushkarev IA. [Isoelectric point of

Bence Jones protein and kidney failure in Bence Jones myeloma]. Klin Med (Mosk).

1984 Apr;62(4):79-84. Russian. PubMed PMID: 6737972.

149: Eichner R, Bonitz P, Sun TT. Classification of epidermal keratins according

to their immunoreactivity, isoelectric point, and mode of expression. J Cell

Biol. 1984 Apr;98(4):1388-96. PubMed PMID: 6201491; PubMed Central PMCID:

PMC2113226.

150: Coward RA, Delamore IW, Mallick NP, Robinson EL. The importance of urinary

immunoglobulin light chain isoelectric point (pI) in nephrotoxicity in multiple

myeloma. Clin Sci (Lond). 1984 Feb;66(2):229-32. PubMed PMID: 6420108.

151: Lanzillo JJ, Stevens J, Tumas J, Fanburg BL. Spontaneous change of human

plasma angiotensin I converting enzyme isoelectric point. Arch Biochem Biophys.

1983 Dec;227(2):434-9. PubMed PMID: 6320726.

152: Yamamoto S, Leonard EJ, Meltzer M. Molecular weight, isoelectric point, and

stability of a murine lymphokine that induces macrophage tumoricidal activity. J

Reticuloendothel Soc. 1983 May;33(5):343-51. PubMed PMID: 6341579.

153: Gachon AM, Kpamegan G, Dastugue B. One step isolation of human lysozyme

using exclusion chromatography based on isoelectric point. Clin Chim Acta. 1983

Apr;129(2):189-95. PubMed PMID: 6851162.

154: Schmidt-Ullrich R, Thompson WS, Kahn SJ, Monroe MT, Wallach DF. Simian virus

40 (SV40)-specific isoelectric point-4.7--94,000-Mr membrane glycoprotein: major

peptide homology exhibited with the nuclear and membrane-associated 94,000-Mr

SV40 T-antigen in hamsters. J Natl Cancer Inst. 1982 Oct;69(4):839-49. PubMed

PMID: 6288993.

155: Weller DL. Isoelectric point and molecular weight of a diaphorase of

Entamoeba invadens. J Parasitol. 1982 Apr;68(2):343-5. PubMed PMID: 6896214.

156: Santagostino A, Giagnoni G, Fumagalli P, Pavesi D, Torretta E. Isoelectric

point determination of human and camel beta-endorphin, alpha-endorphin and

enkephalins. Biochem Biophys Res Commun. 1982 Jan 29;104(2):577-82. PubMed PMID:

157: Shibata K, Constans J, Viau M, Matsumoto H. Polymorphism of the haptoglobin

peptides by isoelectric focusing electrophoresis and isoelectric point

determinations. Hum Genet. 1982;61(3):210-4. PubMed PMID: 7173864.

158: Wang EC, Rose JA. Deoxyribonucleic acid single-strand-specific endonucleases

in human cells: partial purification of a salt-resistant endonuclease with an

acidic isoelectric point. Biochemistry. 1981 Feb 17;20(4):755-8. PubMed PMID:

159: Miwa N, Takayanagi H, Suzuki A. Comparative studies on two active enzyme

forms of human urinary urokinase. I. Purification by serial column chromatography

and homogeneity analyses of molecular weight and isoelectric point. Chem Pharm

Bull (Tokyo). 1981 Feb;29(2):463-71. PubMed PMID: 7023709.

160: Blumberga IA, Iavorkovskiĭ LI. [Determination of the paraprotein isoelectric

point (pI) in the blood and urine by isoelectric focusing on a thin layer of

polyacrylamide gel]. Lab Delo. 1981;(10):598-601. Russian. PubMed PMID: 6170790.

161: McGregor JL, Clemetson KJ, James E, Luscher EF, Dechavannne M.

Characterization of human blood platelet membrane proteins and glycorproteins by

their isoelectric point (pI) and apparent molecular weight using two-dimensional

electrophoresis and surface-labelling techniques. Biochim Biophys Acta. 1980

Jul;599(2):473-83. PubMed PMID: 7407103.

162: Bjørklid E, Little C. The isoelectric point of phospholipase C from Bacillus

cereus. FEBS Lett. 1980 May 5;113(2):161-3. PubMed PMID: 6771157.

163: Revina TA, Podboronov VM, Sycheva IM. [Determination of isoelectric point of

lysozymes isolated from various tick species]. Med Parazitol (Mosk). 1980

Mar-Apr;49(2):33-5. Russian. PubMed PMID: 7393131.

164: Goldman WF, Baptist JN. Isoelectric point electrophoresis: a new technique

for protein purification. J Chromatogr. 1979 Nov 21;179(2):330-2. PubMed PMID:

165: Berg W, Hillvärn B, Arwin H, Stenberg M, Lundström I. The isoelectric point

of thrombin and its behaviour compared to prothrombin at some solid surfaces.

Thromb Haemost. 1979 Oct 31;42(3):972-82. PubMed PMID: 41337.

166: Purtell JN, Pesce AJ, Clyne DH, Miller WC, Pollak VE. Isoelectric point of

albumin: effect on renal handling of albumin. Kidney Int. 1979 Sep;16(3):366-76.

PubMed PMID: 529683.

167: Clyne DH, Pesce AJ, Thompson RE. Nephrotoxicity of Bence Jones proteins in

the rat: importance of protein isoelectric point. Kidney Int. 1979

Sep;16(3):345-52. PubMed PMID: 529681.

168: Anderson NL, Hickman BJ. Analytical techniques for cell fractions. XXIV.

Isoelectric point stadnards for two-dimensional electrophoresis. Anal Biochem.

1979 Mar;93(2):312-20. PubMed PMID: 37775.

169: Evenson MA, Deutsch HF. Influence of fatty acids on the isoelectric point

properties of human serum albumin. Clin Chim Acta. 1978 Oct 16;89(2):341-54.

PubMed PMID: 709880.

170: Bomford A, Berger M, Lis Y, Williams R. The iron content of human liver and

spleen isoferritins correlates with their isoelectric point and subunit

composition. Biochem Biophys Res Commun. 1978 Jul 14;83(1):334-41. PubMed PMID:

171: Morris JA, Stevens AE, Sojka WJ. Isoelectric point of cell-free K99 antigen

exhibiting hemagglutinating properties. Infect Immun. 1978 Mar;19(3):1097-8.

PubMed PMID: 346483; PubMed Central PMCID: PMC422302.

172: Kambara M, Asai T, Kumasaki M, Konishi K. An electrochemical study on the

human dental enamel with special reference to isoelectric point. J Dent Res. 1978

Feb;57(2):306-12. PubMed PMID: 28340.

173: Plancke Y, Dautrevaux M, Biserte G. Human serum hemopexin: direct evidence

for change of its isoelectric point upon heme binding. A new serum protein

fractionation. Biochimie. 1978;60(2):171-5. PubMed PMID: 667170.

174: Chuat JC, Laprevotte I, Bernard C, Pilon C. The isoelectric point of the p30

polypeptide as a marker of mouse endogenous viruses. J Gen Virol. 1978

Jan;38(1):169-73. PubMed PMID: 202669.

175: Plancke Y, Dautrexvaux M, Biserte G. Change of human hemopexin isoelectric

point upon heme binding. FEBS Lett. 1977 Jun 15;78(2):291-4. PubMed PMID: 885254.

176: Sprouse RF. Determination of molecular weight, isoelectric point, and

glycoprotein moiety for the principal skin test-reactive component of

histoplasmin. Infect Immun. 1977 Jan;15(1):263-71. PubMed PMID: 832901; PubMed

Central PMCID: PMC421357.

177: Aita M. [Histochemical study of the isoelectric point of the protein

structures of lymphocytes]. Bull Assoc Anat (Nancy). 1976 Sep;60(170):451-3.

French. PubMed PMID: 69457.

178: Barshteĭn IuA, Danilenko II, Ruban VI. [Detection of the membranous

structures of Salmonella typhimurium at a neutral isoelectric point]. Mikrobiol

Zh. 1976 Jun-Sep;38(4):499-501. Ukrainian. PubMed PMID: 63102.

179: Yasutake A. [Studies on iodide-hydrogen peroxide oxidoreductase in human

parotid saliva--purification, characterization of the enzyme with isoelectric

point of 9.6 and its antibacterial action (author's transl)]. Hiroshima Daigaku

Shigaku Zasshi. 1976;8(1):12-27. Japanese. PubMed PMID: 1069798.

180: Ockert G, Schmidt T. [On the epidemiology of Dientamoeba fragilis Jepps and

Dobell 1918. 4th communication: evidence of Dientamoeba fragilis in Enterobius

eggs using isoelectric point determination]. J Hyg Epidemiol Microbiol Immunol.

1976;20(1):76-81. German. PubMed PMID: 944216.

181: Entlicher G, Kocourek J. Studies on phytohemagglutinins. XXIV. Isoelectric

point and hybridization of the pea (Pisum sativum L.) isophytohemagglutinins.

Biochim Biophys Acta. 1975 May 30;393(1):165-9. PubMed PMID: 237565.

182: Londesborough JC, Hamberg U. The sialic acid content and isoelectric point

of human kininogen. Biochem J. 1975 Feb;145(2):401-3. PubMed PMID: 239685; PubMed

Central PMCID: PMC1165231.

183: Watanabe T. [Studies on exo-type of Beta-N-acetylglucosaminidase in human

parotid saliva. Purification and some properties of the enzyme having isoelectric

point 7.2 (author's transl)]. Hiroshima Daigaku Shigaku Zasshi. 1975;7(2):134-45.

Japanese. PubMed PMID: 1074988.

184: Ericson I. Determination of the isoelectric point of rat liver mitochondria

by cross-partition. Biochim Biophys Acta. 1974 Jul 12;356(1):100-7. PubMed PMID:

185: Harding GB, Burton RM, Stroupe SD, Westphal U. Steroid protein interactions.

XXVIII. The isoelectric point and pH stability of the progesterone-binding

globulin. Life Sci. 1974 Jun 16;14(12):2405-12. PubMed PMID: 4143644.

186: Scuncio G, Cannella C, Grimaldi S, De Santis P. [The isoelectric point of

human thyroglobulin]. Folia Endocrinol. 1974 Apr;27(2):233-45. Italian. PubMed

PMID: 4406494.

187: Ochi Y, Yatani T, Yoshimura M, Miyazaki T, Kaimasu I. [Proceedings:

Isolation of LATS-IgG by isoelectric point electrophoresis]. Nihon Naibunpi

Gakkai Zasshi. 1974 Feb 20;50(2):559. Japanese. PubMed PMID: 4476568.

188: Barni S, Bolognani Fantin AM. [Histochemical determination of the apparent

isoelectric point of the venom of the granulous skin glands of some amphibia].

Riv Istochim Norm Patol. 1974;18(1-4):147. Italian. PubMed PMID: 4470613.

189: Tso MY. Some properties of the nitrogenase proteins from Clostridium

pasteurianum. Molecular weight, subunit structure, isoelectric point and EPR

spectra. Arch Microbiol. 1974;99(1):71-80. PubMed PMID: 4369192.

190: Barni S, Bolognani Fantin AM. [Histochemical determination of the

isoelectric point in the granulose glands of the skin in several species of

amphibia (author's transl)]. Acta Histochem. 1974;50(2):145-54. Italian. PubMed

PMID: 4217538.

191: Ambler J, Miller JN, Johnson P, Orr TS. Characterisation of an allergen

extracted from Ascaris suum. Determination of the molecular weight, isoelectric

point, amino acid and carbohydrate content of the native allergen.

Immunochemistry. 1973 Dec;10(12):815-20. PubMed PMID: 4203356.

192: Chuat JC, Bernard C, Laprevotte I, Seban C, Boiron M. Studies on mouse

sarcoma virus. IV. The isoelectric point of the group-specific antigen. Int J

Cancer. 1973 Nov 15;12(3):742-51. PubMed PMID: 4134010.

193: Strongin AIa, Levin ED, Stepanov VM. [Isoelectric focusing in a stable pH

gradient of 1-3. The isoelectric point of swine pepsin]. Biokhimiia. 1973

Sep-Oct;38(5):1098-100. Russian. PubMed PMID: 4592476.

194: Catsimpoolas N. Transient state isoelectric focusing. Digital measurement of

zone position, zone area, segmental pH gradient, and isoelectric point as a

function of time. Anal Biochem. 1973 Jul;54(1):66-78. PubMed PMID: 4725675.

195: Wynne D, Shalitin Y. Beef liver esterase. I. Isoelectric point and molecular

weight. Arch Biochem Biophys. 1973 Jan;154(1):199-203. PubMed PMID: 4734722.

196: Ghosh S, Basu MK, Schweppe JS. Agarose gel electrophoresis of serum

lipoproteins: determination of true mobility, isoelectric point, and molecular

size. Anal Biochem. 1972 Dec;50(2):592-601. PubMed PMID: 4345791.

197: Caro RA, De Paoli T, Hager AA, Szyszkowsky R, Radicella R. Preparation,

physicochemical properties and biological behaviour of a radiogold colloid

stabilized with gelatine of isoelectric point 9-2. Int J Appl Radiat Isot. 1972

Aug;23(8):382-4. PubMed PMID: 4628126.

198: McGaughey RW, Murray FA. Properties of blastokinin: amino acid composition,

evidence for subunits, and estimation of isoelectric point. Fertil Steril. 1972

Jun;23(6):399-404. PubMed PMID: 5030869.

199: Galdiero F, Morelli F. [The isoelectric point of extracellular proteases of

certain bacterial species]. G Batteriol Virol Immunol. 1972 May-Aug;65(5):140-3.

Italian. PubMed PMID: 4200183.

200: Ui N. Electrophoretic mobility and isoelectric point of hog thyroglobulin.

Biochim Biophys Acta. 1972 Feb 29;257(2):350-64. PubMed PMID: 5022432.

201: Arashima S, Matsuda I. Ornithine transcarbamylase, an isoelectric point (pI)

isozyme in human liver and its deficiency. Biochem Biophys Res Commun. 1971 Oct

1;45(1):145-50. PubMed PMID: 5139917.

202: Entlicher G, Kostír JV, Kocourek J. Studies on phytohemagglutinins. 8.

Isoelectric point and multiplicity of purified concanavalin A. Biochim Biophys

Acta. 1971 Jun 29;236(3):795-7. PubMed PMID: 5559972.

203: Kelly MJ, Reithel FJ. A thermodynamic analysis of the monomer-dimer

association of -lactoglobulin A at the isoelectric point. Biochemistry. 1971 Jun

22;10(13):2639-44. PubMed PMID: 5557805.

204: Hopwood D. Use of isoelectric focusing to determine the isoelectric point of

bovine serum albumin after treatment with various common fixatives. Histochem J.

1971 May;3(3):201-5. PubMed PMID: 4106574.

205: Ceriani RL, Thomas-Morvan C, Nataf BM. Difference in isoelectric point of

27S and 19S thyroidal iodoproteins determined by isoelectric focusing. Proc Soc

Exp Biol Med. 1971 Apr;136(4):1351-4. PubMed PMID: 5554485.

206: Kiriukhin IF. [Isolation of proteins with an isoelectric point of 6.75 from

the liver and a study of their physico-chemical properties]. Biull Eksp Biol Med.

1971 Mar;71(3):48-50. Russian. PubMed PMID: 5563788.

207: Pänkäläinen M, Aro H, Simons K, Kivirikko KI. Protocollagen proline

hydroxylase: molecular weight, subunits and isoelectric point. Biochim Biophys

Acta. 1970 Dec 22;221(3):559-65. PubMed PMID: 5499439.

208: Morikofer-Zwez S, von Wartburg JP, Aebi H. Heterogeneity of erythrocyte

catalase: variability of the isoelectric point. Experientia. 1970 Sep

26;26(9):945-7. PubMed PMID: 5529498.

209: Okada Y, Okunuki K. Studies of cytochrome b-555 from larvae of the housefly,

Musca domestica L. II. Isoelectric point, amino acid composition and circular

dichroic spectra. J Biochem. 1970 Mar;67(3):487-96. PubMed PMID: 5424373.

210: Larcan A, Stoltz JF, Genetet B, Streiff F. [Study of the isoelectric point

of human lymphocytes in Hanks medium]. C R Seances Soc Biol Fil.

1970;164(6):1318-20. French. PubMed PMID: 4252368.

211: Stoltz JF, Streiff F, Larcan A. [Electrokinetic effects in the blood

suspension. I. Elementary theoretical aspects. Modifications during preservation.

Study on the isoelectric point for erythrocytes and blood platelets]. Rev Fr

Transfus. 1969 Dec;12(4):409-21. French. PubMed PMID: 5377273.

212: Khesii IaE, Khemchian LB. [Isoelectric point of ribonucleoproteids of

reticular cell cultures sensitivend resistant to enteroviruses during viral

infection]. Tsitologiia. 1969 Dec;11(12):1559-67. Russian. PubMed PMID: 4986819.

213: Flohé L. [Specific detection of glutathione peroxidase on cellogel

electrophoresis strips and determination of the isoelectric point]. Hoppe Seylers

Z Physiol Chem. 1969 Jul;350(7):856-8. German. PubMed PMID: 4389992.

214: Frömter E, Lüer K. [Concentration and isoelectric point of the fixed charge

in the proximal convoluted tubule of the rat kidney]. Pflugers Arch.

1969;307(2):R76. German. PubMed PMID: 5814893.

215: Polson A, Fawcett C, Katz W. Determination of the isoelectric point of

tanned gelatins. Biochem J. 1969 Jan;111(2):247-8. PubMed PMID: 5763792; PubMed

Central PMCID: PMC1187815.

216: Kubota Y, Ueki H. Determination of the isoelectric point of bovine plasma

albumin by cellulose acetate paper electrophoresis. J Biochem. 1968

Sep;64(3):405-6. PubMed PMID: 5707826.

217: Nichol JC. Sedimentation behavior of chymotrypsinogen A in the vicinity of

the isoelectric point. J Biol Chem. 1968 Aug 10;243(15):4065-9. PubMed PMID:

218: Bishop WH, Richards FM. Isoelectric point of a protein in the crosslinked

crystalline state: beta-lactoglobulin. J Mol Biol. 1968 Apr 28;33(2):415-21.

PubMed PMID: 5700702.

219: Matsuo Y, Horio T. [Experimental physico-chemical methods in biochemistry.

63. Isoelectric point fraction method in the electrophoresis of proteins].

Tanpakushitsu Kakusan Koso. 1967 Sep;12(9):737-48. Japanese. PubMed PMID:

220: Pokidova NV, Buzina TP, Sapozhnikova GA, Ermol'eva ZV. [Determination of

isoelectric point of interferon by means of chromatography on KM-sephadex].

Antibiotiki. 1967 Jul;12(7):547-9. Russian. PubMed PMID: 5595915.

221: SOPHIANOPOULOS AJ, SASSE EA. ISOELECTRIC POINT OF PROTEINS BY DIFFERENTIAL

CONDUCTIMETRY. J Biol Chem. 1965 Apr;240:PC1864-6. PubMed PMID: 14285536.

222: BODO G, JUNGWIRTH C. [THE DETERMINATION OF THE ISOELECTRIC POINT OF CHICK

INTERFERON BY ZONAL ELECTROPHORESIS IN DENSITY GRADIENTS]. Biochem Z. 1964 Jul

8;340:56-9. German. PubMed PMID: 14317952.

223: NISHIZAKI S. [PHARMACEUTICAL AND CHEMICAL STUDIES ON INSULIN. (30) ON

N-TERMINAL AMINO ACIDS AND CHEMICAL STRUCTURES OF BONITO AND TUNNY FISH INSULINS

PURIFIED BY PRECIPITATION AT ISOELECTRIC POINT]. Eisei Shikenjo Hokoku. 1963

Oct;81:30-3. Japanese. PubMed PMID: 14162026.

224: ALESHIN BV, KRIVOBOK IV. [SHIFTS IN THE ABSORPTIVE CAPACITY AND ISOELECTRIC

POINT OF THYROID CELLS DURING THE ACTION OF 6-METHYLTHIOURACIL]. Biull Eksp Biol

Med. 1963 Aug;56:101-6. Russian. PubMed PMID: 14090457.

225: SVENSMARK O, KRISTENSEN P. Isoelectric point of native and sialidase-treated

human-serum cholinesterase. Biochim Biophys Acta. 1963 Mar 12;67:441-52. PubMed

PMID: 13979553.

226: SCHLEYER F. [Studies on determination of the time of death by measurement of

the isoelectric point of extracts from the organs of cadavers]. Zentralbl Allg

Pathol. 1961 May 31;102:272-4. German. PubMed PMID: 13747812.

227: BUTLER LO. Studies on the preparation and isoelectric point of

staphylococcal alpha-haemolysin. Biochem J. 1959 Jan;71(1):67-73. PubMed PMID:

13628534; PubMed Central PMCID: PMC1196750.

228: JACKSON DS, NEUBERGER A. Observations on the isoionic and isoelectric point

of acid-processed gelatin from insoluble and citrate-extracted collagen. Biochim

Biophys Acta. 1957 Dec;26(3):638-9. PubMed PMID: 13499423.

229: LAMPRECHT L. [Physiology of true house fungus; consumption of anorganic

nitrogen compounds in relation to the concentration, pH value and isoelectric

point]. Arch Mikrobiol. 1957;27(2):182-218. German. PubMed PMID: 13522135.

230: KENNEDY ER, WOODHOUR AF. Quantitative studies of differential staining

reactions. I. The effect of pH on the quantity of dye retained by bacteria and

the apparent isoelectric point. J Bacteriol. 1956 Oct;72(4):447-50. PubMed PMID:

13366944; PubMed Central PMCID: PMC357932.

231: KELENYI G. [Determination of the isoelectric point of tissues in

hematological histology]. Kiserl Orvostud. 1956 Mar;8(2):192-6. Hungarian. PubMed

PMID: 13332892.

232: SCHUMMELFEDER N. [Effect of the buffer solution on the chromatic

determination of the isoelectric point of tissue elements]. Z Zellforsch Mikrosk

Anat. 1956;44(5):488-94. German. PubMed PMID: 13393351.

233: SCHUMMELFEDER N, STOCK KF. [Determination of the range of the pH change

(isoelectric point) of tissue elements with fluorchrome acridine orange]. Z

Zellforsch Mikrosk Anat. 1956;44(3):327-38. German. PubMed PMID: 13338629.

234: RAACKE ID, LI CH. Corticotropins (ACTH). VI. Isoelectric point of

alpha-corticotropin as determined by zone electrophoresis on starch. J Biol Chem.

1955 Jul;215(1):277-85. PubMed PMID: 14392162.

235: LARGIER JF. A rapid method for isoelectric point determination. Biochim

Biophys Acta. 1955 Jul;17(3):341-5. PubMed PMID: 13239689.

236: WEIR JH, TSUJI FI, CHASE AM. The isoelectric point of Cypridina luciferase.

Arch Biochem Biophys. 1955 May;56(1):235-9. PubMed PMID: 14377569.

237: PRICE WC. Genetic composition in relation to isoelectric point and

serological reaction of strains in tobacco mosaic virus. Trans N Y Acad Sci. 1954

Feb;16(4):196-201. PubMed PMID: 13187557.

238: TOMCSIK J. [An effect of proteins on bacteria which is dependent upon

isoelectric point]. Bibl Paediatr. 1954;58:410-9. German. PubMed PMID: 13229951.

239: HARDEN VP, HARRIS JO. The isoelectric point of bacterial cells. J Bacteriol.

1953 Feb;65(2):198-202. PubMed PMID: 13034716; PubMed Central PMCID: PMC169666.

240: PASYNSKII A, BLOKHINA V. [Displacement of isoelectric point in proteins in

their deformation]. Dokl Akad Nauk SSSR. 1952 Oct 21;86(6):1171-3. Undetermined

Language. PubMed PMID: 12998569.

241: IMAI T, KUBOTA S. [Growth of stomach cancer and isoelectric point of cancer

cells]. Gan. 1952 Sep;43(2-3):201-2. Undetermined Language. PubMed PMID:

242: DOERING P. [Investigations on the isoelectric point of erythrocytes]. Naunyn

Schmiedebergs Arch Exp Pathol Pharmakol. 1952 Sep;216(4):410-4. Undetermined

Language. PubMed PMID: 12986750.

243: INGRAM VM. Isoelectric point of chymotrypsinogen by a Donnan equilibrium

method. Nature. 1952 Aug 9;170(4319):250-1. PubMed PMID: 12993124.

244: FRITZE E, DOERING P. [The electric charge and the isoelectric point of the

blood cells and their relation to the cell function]. Klin Wochenschr. 1952 Jan

1;30(1-2):19-22. Undetermined Language. PubMed PMID: 14939637.

245: BIER M, NORD FF. On the mechanism of enzyme acition. XLVI. The effect of

certain ions on crystalline trypsin and reinvestigation of its isoelectric point.

Arch Biochem Biophys. 1951 Sep;33(2):320-32. PubMed PMID: 14886014.

246: SARKAR NK. Effect of salts on the isoelectric point of myosin. Enzymologia.

1951 Jun 15;15(1):1-2. PubMed PMID: 14896889.

247: JANUS JW, KENCHINGTON AW, WARD AG. A rapid method for the determination of

the isoelectric point of gelatin using mixed bed deionization. Research. 1951

May;4(5):247-8. PubMed PMID: 14844834.

248: RAVICH-SHCHERBO MI, ZAKHAROVA NA. [Isoelectric point and serum protein

coagulation threshold in cancer]. Arkh Patol. 1951 Mar-Apr;13(2):64-9.

Undetermined Language. PubMed PMID: 14858158.

249: SCHRIEVER H, WESTPHAL E. [Ampholyte nature and isoelectric point of the cell

membrane as it affects concentration potential]. Pflugers Arch.

1951;253(4-5):402-11. Undetermined Language. PubMed PMID: 14911350.

250: CARPENTER DC, SMITH WC. Separation of a crystalline globulin from tomato

juice and determination of its isoelectric point. Arch Biochem. 1949

Aug;23(1):45-8. PubMed PMID: 18135761.

251: TARASSEVICH LM. Determination of the isoelectric point of virus proteins by

the staining technique. Dokl Akad Nauk Soiuza Sov Sotsialisticheskikh Resp.

1945;47(9):666-8. PubMed PMID: 21026721.

252: Fishman M, Moyer LS. THE CHLOROPHYLL-PROTEIN COMPLEX : I. ELECTROPHORETIC

PROPERTIES AND ISOELECTRIC POINT. J Gen Physiol. 1942 May 20;25(5):755-64. PubMed

PMID: 19873311; PubMed Central PMCID: PMC2142537.

253: Heidelberger M, Pedersen KO. THE MOLECULAR WEIGHT AND ISOELECTRIC POINT OF

THYROGLOBULIN. J Gen Physiol. 1935 Sep 20;19(1):95-108. PubMed PMID: 19872918;

PubMed Central PMCID: PMC2141410.

254: Fischgold H, Chain E. The spontaneous decomposition of lecithin and its

bearing on the determination of the isoelectric point. Biochem J.

1934;28(6):2044-51. PubMed PMID: 16745608; PubMed Central PMCID: PMC1253437.

255: Andrzejewski H. Effect of addition of salts on the isoelectric point of

proteins. III. Biochem J. 1932;26(5):1397-405. PubMed PMID: 16744958; PubMed

Central PMCID: PMC1261051.

256: Hitchcock DI. THE ISOELECTRIC POINT OF A STANDARD GELATIN PREPARATION. J Gen

Physiol. 1931 Jul 20;14(6):685-99. PubMed PMID: 19872615; PubMed Central PMCID:

PMC2141050.

257: Straup D. THE FLOCCULATION OF GELATIN AT THE ISOELECTRIC POINT. J Gen

Physiol. 1931 May 20;14(5):643-60. PubMed PMID: 19872613; PubMed Central PMCID:

PMC2141126.

258: Terroux KG. The isoelectric point of sheep oxyhaemoglobin determined by

titration curves with the glass electrode. J Physiol. 1931 Mar 23;71(3):323-30.

PubMed PMID: 16994180; PubMed Central PMCID: PMC1403058.

259: Przyłecki SJ, Giedroyć W. Effect of addition of salts on the isoelectric

point of proteins. II. Biochem J. 1931;25(5):1734-5. PubMed PMID: 16744740;

PubMed Central PMCID: PMC1260802.

260: Przyłecki SJ. Effect of addition of salts on the isoelectric point of

proteins. I. Biochem J. 1931;25(3):713-27. PubMed PMID: 16744632; PubMed Central

PMCID: PMC1260688.

261: Abramson HA. ELECTROKINETIC PHENOMENA. III : THE "ISOELECTRIC POINT" OF

NORMAL AND SENSITIZED MAMMALIAN ERYTHROCYTES. J Gen Physiol. 1930 Nov

20;14(2):163-77. PubMed PMID: 19872579; PubMed Central PMCID: PMC2141106.

262: Hitchcock DI. THE COMBINATION OF GELATIN WITH HYDROCHLORIC ACID : II. NEW

DETERMINATIONS OF THE ISOELECTRIC POINT AND COMBINING CAPACITY OF A PURIFIED

GELATIN. J Gen Physiol. 1929 Mar 20;12(4):495-509. PubMed PMID: 19872477; PubMed

Central PMCID: PMC2323736.

263: Stearn AE. AMPHOTERIC BEHAVIOR OF COMPLEX SYSTEMS : IV. NOTE ON THE

ISOELECTRIC POINT AND IONIZATION CONSTANTS OF SULFANILIC ACID. J Gen Physiol.

1927 Jan 20;10(3):379-83. PubMed PMID: 19872329; PubMed Central PMCID:

PMC2140914.

264: Hitchcock DI. THE ISOELECTRIC POINT OF GELATIN AT 40 degrees C. J Gen

Physiol. 1924 Mar 20;6(4):457-62. PubMed PMID: 19872087; PubMed Central PMCID:

PMC2140649.

265: Loeb J. IONIZING INFLUENCE OF SALTS WITH TRIVALENT AND TETRAVALENT IONS ON

CRYSTALLINE EGG ALBUMIN AT THE ISOELECTRIC POINT. J Gen Physiol. 1922 Jul

20;4(6):759-68. PubMed PMID: 19871973; PubMed Central PMCID: PMC2140540.

266: Loeb J. THE ELIMINATION OF DISCREPANCIES BETWEEN OBSERVED AND CALCULATED

P.D. OF PROTEIN SOLUTIONS NEAR THE ISOELECTRIC POINT WITH THE AID OF BUFFER

SOLUTIONS. J Gen Physiol. 1922 May 20;4(5):617-9. PubMed PMID: 19871960; PubMed

Central PMCID: PMC2140519.

267: Coulter CB. THE ISOELECTRIC POINT OF RED BLOOD CELLS AND ITS RELATION TO

AGGLUTINATION. J Gen Physiol. 1921 Jan 20;3(3):309-23. PubMed PMID: 19871866;

PubMed Central PMCID: PMC2140442.

268: Lloyd DJ. Note on the Production of a Contracting Clot in a Gel of Gelatin

at the Isoelectric Point. Biochem J. 1920 Oct;14(5):584-5. PubMed PMID: 16742914;

PubMed Central PMCID: PMC1258922.

269: Loeb J. AMPHOTERIC COLLOIDS : II. VOLUMETRIC ANALYSIS OF ION-PROTEIN

COMPOUNDS; THE SIGNIFICANCE OF THE ISOELECTRIC POINT FOR THE PURIFICATION OF

AMPHOTERIC COLLOIDS. J Gen Physiol. 1918 Nov 20;1(2):237-54. PubMed PMID:

19871741; PubMed Central PMCID: PMC2140300.