Page 411-420

Yes Jet, they do appear to be an elitist org. But
 someone has to pony up the bucks for "validation" of a particular
compound, and recoup the money by whatever means. Unless the gov funds
the effort, and requires public domain....that's the model we are stuck
with.

But I didn't mean for that to be a defacto example. Dr. Strum has
indicated he'd work with any oncs & orgs with a sincere scientific
mind set.

Another parrallel example of a funding/trial effort could be something like Give1forDad: http://give1fordad.com/about-the-study/

Or perhaps the Cures Within Reach/ Cures Accelarator platform:

http://www.cureswithinreach.org/

http://cureaccelerator.org/

In any case....was just curious if the "Strum Project" still had some legs to it.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Sun Oct 02, 2016 06:22 PM
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Dear MnMKona,
I am not sure, but it was my impression from my last contact with Dr
Strum that he was quite disappointed about getting 3BP on the move.
Maybe things were more complicated than he envisioned. And since he has
not reacted up to this moment, himself, is a clear indication to me that
 his laudable efforts have hit a snag. Which would be a pity for science
 and patients.
kind regards, finder.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Sat Oct 15, 2016 06:28 PM
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I do know that Dr Ko is trying to raise money for clinical trials via the Ko foundation.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by MnMKona on Sat Oct 15, 2016 11:17 PM
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Yes & Ko has recently published a couple of papers.....stirring up interest?

Separately, this recent paper suggests interest by some folks at UC Irvine.

https://cancerandmetabolism.biomedcentral.com/articles/10.11

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by sumeetnag on Sun Oct 16, 2016 02:41 AM
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From my knowledge a team of
experts was created, protocol created using experience of those with
3bp. All the doctors were asked to present patients keen to undergo the
trial.
So far to my knowledge only one patient went through this process, there
 were challenges with the delivery mode. Eventually it did not work for
this pancreatic cancer patient. Dr.Strum eventually gave up due to lack of patients being put forward by the Doctors.
What is the update from the Dutch-German clinics? They practised it in various forms of delivery.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Mon Oct 17, 2016 08:51 PM
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Thank you everyone for your recent posts to the thread, they have really cheered me up.

The patients who went to the Bracht clinic were honorary friends of
the thread and I am sad that 3-BP caused the tragedy that it did. The
entire motivation of our thread has been to try and help these patients.

There is now a growing backlog of items of interest regarding 3-BP
that I am raring to post to the thread. However, until the official
investigation report is released we will be in somewhat of a holding
pattern.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by MnMKona on Mon Oct 17, 2016 09:01 PM
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Ugh..Dr Strum had mentioned that in the past he had this problem of enrollment, whereby the oncs provide the population.

But in a small clinic setting, there's not enough of an initial population to screen through & qualify.

He mentioned to me he thought that wouldn't be a problem with 3BP
given all the "underground" interest. To bad it wasn't made better known
 of. That's a real shame.
And I wonder what the challenge of the delivery mode was?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by lolopuffin on Mon Oct 17, 2016 09:12 PM
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I've missed something.  What tragedy?  What happened?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Mon Oct 17, 2016 10:48 PM
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lolopuffin, there was a 3-BP tragedy at the
Bracht clinic on the German border. At least 3 of the patients that were
 treated there some time near the end of July passed away within days of
 treatment. It is still being investigated, though the heresay evidence
to date suggests that a change in 3-BP supplier possibly in combination
with the way in which it was formulated lead to an overdosing of 3-BP.

Much of the 3-BP community has been in stand by mode waiting for a
conclusive report about the incident. Some of the leading treatment
clinics are no longer advertising 3-BP services.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Tue Oct 18, 2016 08:32 PM
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The psiram blog is doing a great job of reporting
 on 3-BP and the Bracht incident. Something that I noticed in their
reports was that a German pharmacy had produced 1400 ampules of 3-BP.{I
think this might be the one that D has referred to, Hesse?}

This is quite an astonishing amount of production for a chemical that
 has never even started a clinical trial. When you go online to Chinese
supplier website, you can buy kilogram scale quantities of
pharmaceutical grade 3-BP. I think it should very obvious to all on our
thread that we urgently need clinical trial evidence of 3-BP. 3-BP has
already rolled out on an industrial scale and it seems only fair that
those who are even now being treated with it have  objective research
that can inform their decision to try it. That 3-BP should be in the
clinic is not headline worthy news on our thread, though the scale of
current gray market usage of it IS news to me.

https://www.psiram.com/ge/index.php/3-Bromopyruvat\_Therapie

https://www.psiram.com/ge/index.php?title=Klaus\_Ross&typ

"According to Deutsche Apotheker-Zeitung have the pharmacy in the past more than 1,400 3-BP - ampoules manufactured and sold by prescription.After the deaths the pharmacy had to assure the authorities, no longer produce the drug as a formulation, and to start a recall."

Original German "Nach Angaben der Deutschen Apotheker-Zeitung habe
die Apotheke in der Vergangenheit mehr als 1400 3-BP - Ampullen auf
Rezept hergestellt und verkauft.Nach
 den Todesfällen habe die Apotheke den den Behörden zusichern müssen,
das Arzneimittel nicht mehr als Rezeptur herzustellen, und einen Rückruf
 zu starten."

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Oct 22, 2016 08:07 PM
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finder, I know that we are all in quiet mode
though there is just so many interesting things going on with 3-BP, so I
 though I would break the silence.

I only have the abstract for the below, yet it seems to be referring
to you exact idea of isotoping 3-BP. It would be very interesting to see
 what a scan of isotopic 3-BP might show in vivo regarding possible
indications of therapeutic target engagement.

"2-deoxyglucose is not a specific glycolytic inhibitor" Anyone have a
 explanation of this statement. It seems very mysterious to me.

 http://www.proteomicsconference.com/abstract/2016/systematic

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Oct 22, 2016 08:24 PM
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I want to post some of the 3-BP items that have been piling up so that they don't zap into the unfindable box.

I came across this longecity thread that appears quite related to
much of our discussions. There are some highly interesting comments on
this thread. First the thread was motivated by the idea of creating the
conditions for mitophagy (eliminating mitochondria especially defective
ones). I had no idea that such a process was even possible. We have
noted on the thread before PQQ as a mitogenesis agent, though this was
not mentioned with respect to a comprehensive mitophagy strategy. What I
 am wondering is whether mitophagy plus mitogenesis might be helpful in
cancer treatment.  "nicotinamide (and also niacin and nicotinamide
riboside) can increase this ratio and dramatically increase mitophagy— "

We have noted on the thread before that the NAD+ to NADH ratio is a centrally important redox indicator for the cell,

"The balance between the oxidized and reduced forms of nicotinamide adenine dinucleotide is called the *NAD*+/*NADH ratio*. This *ratio* is an important component of what is called the *redox state* of a cell, a measurement that reflects both the metabolic activities and the health of cells." wiki

though I had no idea that this indicator had further importance in
cell behavior, apparently it does :  "Cells use the ratio of NAD+ to
NADH (the oxidized and reduced forms of nicotinamide adenine
dinucleotide) as a signal to begin mitophagy."

http://www.longecity.org/forum/topic/86297-a-protocol-to-upg

The motivation would be that stressing OXPHOS and then possibly
glycolysis with an anti-glycolytic might greatly stress cancer cells. It
 has been noted on our thread that some cancers (e.g. ccRCC) appear to
be highly deficient in mitochondria. A mitophagy strategy might be
especially powerful in such contexts.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Oct 22, 2016 08:34 PM
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"CRC cell exposure to acetate upregulates the
expression of MCT1, MCT4 and CD147, while promoting MCT1 plasma membrane
 localization. We also observed that acetate increases CRC cell
glycolytic phenotype and that acetate-induced apoptosis and
anti-proliferative effect was potentiated by 3BP. Our data suggest that
acetate selectivity towards CRC cells might be explained by the fact
that aquaporins and MCTs are found overexpressed in CRC clinical cases."

I am not sure whether this one has been posted to the thread yet,
though it always seems worthwhile posting something that might
upregulate MCT-1.

https://www.ncbi.nlm.nih.gov/pubmed/27661124

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Oct 22, 2016 08:41 PM
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Another one I wanted to post was from the wikia.
Someone posted that the 3-BP spectro analysis idea was reasonable (think
 that one was yours finder).

It is always great to have those with a chemical background giving us
 some confirmation. This got me thinking that this might be a great one
for the 3-BP clinics to use as a simple cheap check to ensure that they
were using the right 3-BP dosage. Toss some 3-BP into a curvette and see
 what the concentration was. Perhaps such a simple procedure could avoid
 dosing problems in the future. Waiting hours after preparing a 3-BP
batch for treatment introduces too many variables and thus too much
uncertainty regarding how much active 3-BP was in fact present. A simple
 check might help reduce the risk.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Oct 22, 2016 09:48 PM
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Wow! We really need to keep up wih what's going
on out there. I am here for the people to make sure that we're not so
clued out that we have no idea what's happening.

So, it looks like they started an anti-aging clinical trial several
months ago. This has been shown to reverse key metabolic features of
aging in mice in a week. There are so many players in motion with this
that I would call on the thread to help think what this would mean for
our metabolic approach to cancer. How might NMN fit into our
perspective?

http://www.dmarge.com/2016/07/anti-aging-drug-humans.html

https://en.wikipedia.org/wiki/Nicotinamide\_mononucleotide#ci

If you want some, it's online, apparently it has been out there for a while now.

The thread has discussed the possibility of shifting NAD+ levels, though this was considered unlikely. Perhaps not.

"We trace the cause to an alternate PGC-1α/β-independent pathway of
nuclear-mitochondrial communication that is induced by a decline in
nuclear NAD+and the accumulation of HIF-1α under normoxic conditions,
with parallels to Warburg reprogramming. Deleting SIRT1 accelerates this
 process, whereas raising NAD+levels in old mice restores mitochondrial
function to that of a young mouse in a SIRT1-dependent manner. Thus, a
pseudohypoxic state that disrupts PGC-1α/β-independent
nuclear-mitochondrial communication contributes to the decline in
mitochondrial function with age, a process that is apparently
reversible."

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4076149/#R55

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Oct 22, 2016 09:54 PM
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One of my posts has just went to moderation. I think it's too important to wait, so here's the edited version.

Looks like there might be an anti-aging treatment in the pipe. We
have talked about possibly changing NAD+ levels, apparently someone has
figured out how to make this happen.

"We trace the cause to an alternate PGC-1α/β-independent pathway of
nuclear-mitochondrial communication that is induced by a decline in
nuclear NAD+and the accumulation of HIF-1α under normoxic conditions,
with parallels to Warburg reprogramming. Deleting SIRT1 accelerates this
 process, whereas raising NAD+levels in old mice restores mitochondrial
function to that of a young mouse in a SIRT1-dependent manner. Thus, a
pseudohypoxic state that disrupts PGC-1α/β-independent
nuclear-mitochondrial communication contributes to the decline in
mitochondrial function with age, a process that is apparently
reversible."

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4076149/#R55

NMN is already available online.

Would love ot hear thread comments on how this might be integrated into our anti-metabolic approach to cancer.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Oct 22, 2016 11:19 PM
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This article extensively discusses NAD/NADH ratio and there is a mention of 3BrOP.

http://www.anti-agingfirewalls.com/2015/05/01/part-4-of-the-

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by kccoc on Sat Oct 22, 2016 11:53 PM
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Not sure if this article has already been posted here, if not, just fyi;

"BP-induced cytotoxicity was independent of p53.
When combined with cisplatin or oxaliplatin, BP led to massive cell
death. The anti-proliferative effects of low-dose platinum were
strikingly potentiated also in resistant p53-deficient cells. Together
with the reported lack of toxicity, this indicates the potential of BP
as a clinical chemopotentiating agent."

"3-Bromopyruvate as inhibitor of tumour cell energy metabolism and chemopotentiator of platinum drugs (2008)" - www.ncbi.nlm.nih.gov /pubmed/19383331

Therefore, instead of 3BP as a monotherpay, might 3BP not be positioned as an 'enhancer' to pharma-standard chemo drug?

Much like "pre-med' to prevent nauseas or swelling, can 3BP be use in
 situation where a lower dose of chemo drug is necessary for patients
that could not tolerate toxicity at standard dosing regimen? or even for
 cost consideration, to be able to use less of the chemo drug.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Oct 23, 2016 03:42 PM
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kc, this is a very good point that the thead has
largely ignored up to this point. Typically cancer patients want to
follow their doctor's advice which usually means substantial amounts of
chemotherapy. There are probably now hundreds of thousands of American
cancer patients obediently adhering to this treatment plan. Few of them
would even consider 3-BP as a potential treatment.

3-BP uses a trojan horse strategy to enter cancer cells, perhaps it
could also use this strategy to make it to market. All those hundreds of
 thousands of patients could keep their chemo and possibly potentiate it
 with some 3-BP. It would be a great way for 3-BP to finally enter
clinical trials.

I guess the reason why we did not wind up with those clinical trials
is that the original 3-BP patient reports were so dramatic. The liver
patient was treated with 3-BP monotherapy and the melanooma patient was
treated with combo 3-BP paracetamol. Usually doctors only introduce a
new drug as an addition to standard of care chemo. This generally leads
to muted expectations that the new drug will be effective. However, most
 of the focus has shifted to 3-BP as the central treatment with other
add ons to intensive its effects.

Most of the chemo 3-BP studies were done a few years ago. It would be
 interesting to hear if some on the thread have tried this combo.

I was interested to see how 3-BP through depleting ATP helped to
reduce multi-drug resistance. Cancer cells can simply pump out chemo.
However, with ATP they can't.

https://www.ncbi.nlm.nih.gov/pubmed/24463111

Figure 3A of the article you cited shows the cell cycle stage
proportions of cells treated with 3-BP. This has me wondering whether
some sort of 3-BP cell cycle combo might be reasonable. Also I am
wondering about the chronobiology side of 3-BP. Is there an exploitable
weakness of the metabolism of cancer cells through the cycle of the day.
 For example, do cancer cells have constantly high energy demands or
just at certain points of the day. It would be quite interesting to know
 whether normal cells power down at night while cancer cells might be
relatively more active.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by MnMKona on Sun Oct 23, 2016 06:45 PM
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Jcancom

The chronomodulated chemotherapy strategy is not new, "The schedule
for the release of your medication is based on the biological uniqueness
 of the drug being given, the time when specific cancer cells divide
[power up?] and are more vulnerable to cell death, and the time when
healthy cells are at rest and least sensitive to toxicity from
chemotherapy."

http://www.blockmd.com/what-is-integrative-cancer-care/chron

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Oct 23, 2016 07:52 PM
 Quote | Reply

MnM, exactly, exactly!

Chronobiology has been around for decades. I am wondering what
happens when they do the chrono research for 3-BP. I do not remember any
 of the 3-BP studies that I have read considering time of day. I would
be very interested to see what the result would be if they were to
systematically manipulate variables such as cell cycle stage, time of
day, ambient temperature etc. .

My hunch is that there could be some room to improve 3-BP's
effectiveness. Maximally knocking down ATP levels in cancer cells might
improve outcomes. With heart attack they have found that reducing body
temperature by even a few degrees can lead to better outcomes. Possibly
reducing body temperature before anti-metabolic treatment could also be
helpful (put the body in suspended animation while the cancer cells do
not get the message).

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by MnMKona on Sun Oct 23, 2016 09:24 PM
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I'm not sure if this statement, "By comparing the
 levels of metabolites in tumors with those in normal tissues, they were
 able to chart the rise and fall of these chemicals." reflects what
comparative time frames (if any). Or if 3BP or it's "effector" compounds
 are even included in their panel makeup (one would think it is or
should be).
Using this tool would require tissue samples taken at different times of day (not likely to happen).
So how would one mimic/translate daily rhythms in petri dish?
http://www.cancertherapyadvisor.com/renal-cell-carcinoma/kid
But
 Dr. Block has done it (and/or whoever) in different disease types &
 chemos, so it's possible! Using population studies to verify.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Mon Oct 24, 2016 07:19 PM
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MnM, I am just not sure whether the Chrono
studies that have been done to date for traditional chemotherapy truly
applies to anti-metabolics. We might need a new branch of Chrono
therapy: Chronometabolic therapy.

Chemotherapies are targeting different mechanisms of action which
probably are related to circadian cycles in a different way than with
3-BP and other such drugs. There might be a magic moment sometime during
 the circadian cycle where 3-BP treatment could have exceptional
effectiveness. It is very frustrating that this research does not seem
to have been done. There is so much translational research that is still
 on the table waiting to be done for 3-BP.

{finder, is probably out there reading this so he can help us out.}
It is possible that a non-invasive scan could be done that would show
how tumor metabolism changed through the day. Research might then use
such scans to coordinate treatment.

I think it is fair to suggest that in the published patient reports
to date of 3-BP the circadian rhythm of the hospitals were taken into
account more so than that of the patients. Most of the patients appear
to have been treated between noon and 3 PM. This would exactly fit the
shift rotation of these hosptials.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Mon Oct 24, 2016 07:23 PM
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I suggested combo chemo 3-BP in a previous post.
Here is a cautionary tale with combo chemo DCA. It is sobering to
realize that even with an entire team of highly qualified researchers
within a clinical trial context things can go wrong: unexpected
synergies might occur that are difficult to understand even in
retrospect.

 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939783/

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Mon Oct 24, 2016 07:32 PM
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Here is another chemical called DCA with an
interesting anti-cancer potential. D has posted about using
acidification strategies as a treatment strategy. I would love to hear
his comments on how this DCA might be combined to enhance the effect.
Perhaps locking in lactic acid?   Somewhat surprising how large the ATP
decrease was with DCA treatment.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3161789/#pone.0

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by MnMKona on Mon Oct 24, 2016 10:06 PM
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Jcan
"Chronometabolic therapy" has a nice intriguing ring to it!
I think the chronochemo studies might at least provide a tentative template for the metabolic approach.
Never really crossed my mind how those studies were designed.
Certainly "mechanism of action" requires looking at all aspects of cell behaviour, including circadium rhythm.
Interestingly,
 there has been a vast array of new "cancer metabolism", conferences,
orgs, a facebook page, web sites, etc popping up.
The metabolic modality of cancer seems to be busting wide open.......3BP HAS to in the mix(es) somewhere.
BTW, Dr. Ko will be speaking at this one in Feb.
http://metabolictherapeuticsconference.com/speakers/

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Meech90 on Tue Oct 25, 2016 11:10 AM
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Some interesting speakers that I know work with a
Keto diet, in specific for cancer therapy. Dr. Seyfried and Miriam
Kalamian are both heavily interested in that field and have a ton of
experience with patients. Andrew Scarborough is an interesting one for
people to check out. I believe he's achieved remission with a Keto diet
for GBM.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Tue Oct 25, 2016 03:29 PM
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DEar Jcancom,
I have been on holidays for the past two weeks, so that is why I have
been silent most of the time. As for the linked paper on proteomics and
isotopes, it looks as if the abstract has been retracted. Strange. The
use of isotopes in proteomics resesarch is 99% stable isotopes, since
detection is mostly by advanced mass spectrometers. I am currently
working on the analysis of a 3-BP related dataset, and there is no need
to use any isotopes. Brome has two stable isotopes, 79Br and 81Br, which
 occur at about 50% each in nature. Fragments of proteins that would
contain bromine (very unlikely to occur) will have their peaks split up
with a mass difference of 2 daltons if one bromine would have bound to
the fragment. For more, patterns become increasingly complex, but they
will stand out as a sore tooth during analysis.
The term "glycolytic inhibitor" is not appropriate for 2-DG, since it
works by competing with glucose for the phosphate (ATP) in a cell, but
does not inhibit the enzyme in any other way. Just semantics, to my
opinion.

kind regards,
finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by alternmed on Thu Oct 27, 2016 08:10 PM
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Dear all

a sad out of subject ,daniel has lost his wife.

i wanted to post my condoleances to his site but i am getting blocked from some reasons..

I hope he and others fighters will continue as there is many will need all help that can provided in research...

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Meech90 on Thu Oct 27, 2016 08:33 PM
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I'm incredibly sorry for your loss Daniel. That is awful news. If you need anything at all don't hesitate.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Emad-Ly on Fri Oct 28, 2016 02:58 PM
 Quote | Reply

I can't believe what happened with Daniel

my heart and soul are broken for this tragedy

he was a true hero to us , giving us his limited time just to help desperate people like us

he is like a mentor to me , i learned from him how to apply these
alternative treatments to my very important patient (my mother)

i even started to administrate a high risk treatments
like Salinomycin to my mother all thanks to God and thanks to Daniel who
 shared all this information only to help my mother and my entire family

and of course , all thanks to his dear wife , without her there wouldn't be a Daniel helping us with his precious time

I have been inspired by him and his wife all the time , and kept strong and taking risks trying to make things better

now after what happened i felt like I lost a true family member , i wish i can be with you now Daniel

im so sorry for your lost , i will never forget your great help

your wife and all the warriors who lost against cancer will live in our hearts

please stay strong

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by jetsparkle on Fri Oct 28, 2016 03:31 PM
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Oh, no!!!!!! What a terrible tragedy!!!

My dearest Daniel, I know you loved and will always continue to love
your precious Miha.   What a wonderful person she will always be in our
hearts==and how we all admire your deep love and actions as a true
warrior for her during these past few years.  She knew the depth of your
 devotion for her, Daniel--and how exceptional a husband and person you
are indeed.

I just cannot adequately express my sense of pain and heartfelt
sympathy upon her passing and  the terrible loss you must be feeling
right now.  You will always be in my heart and I will pray for you as
you try to cope with all of this.

Remember her great love for you and the fact that because you feel
the same way for her that she had a few extra years of life--but how I
wish there could have been many, many more for her.  You were her
steadfast warrior--never forget that.  And you shared your time and
research with so many during all of this--showing the greatness of your
spirit and heart.  Never change--continue to be the gentle, open person
you are.  Miha would have wanted that, I believe.  And she herself
shared you with us--showing her kind  spirit during her travails, too.

I will always have you in my heart, Daniel--please know that many
care about you and will  be wishing you peace of heart and of mind.

Sending your my heartfelt and deepest condolences and hopes,

Jetsparkle

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by peggyznd on Fri Oct 28, 2016 03:46 PM
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Dear Daniel, I am so saddened to hear of the loss
 of your wife, whom you obviously loved dearly and championed for her.
 The terrible grief you must feel is shared by all of us, and do hope
that you have friends and family near by you at this time.

Sincerely,

Peggy

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by falconet on Fri Oct 28, 2016 04:52 PM
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words fail to descirbe how tragic this feels to
me. I don't think I can ever be a happy person again even if my own mom
survives this battle. something lost in me forever as I had never seen a
 love like this. I believe the most precious gift, aside from all the
valuable information he shared with us, was to have faith in love.
dear Daniel, forgive me that I never had anything to offer in return.
Pouya.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by ipappas on Fri Oct 28, 2016 05:18 PM
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Dear Daniel, I am deeply saddened about your
great loss... accept my sincere condoleances, it is terribly painful.
We, your friends since 3 years on this thread, we share and feel the
pain.. as if it were a family member. For 3 years you have taught and
encouraged us with your countless, balanced, wise and thoughtful posts
here and on your website. Thank you so much. You achieved alot for your
wife and learned a lot on this path, which I hope will lead one day soon
 to no more lost battles... Once you recover, please carry on helping
those in need by sharing your research! With sincere friendship and
sympathy,
Ion.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Fri Oct 28, 2016 06:36 PM
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Dear Daniel,
this is a sad message. We hope that Miha may rest in peace and that you will find the strength to cope with this terrible loss.
kind regards,
finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Fri Oct 28, 2016 06:55 PM
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Dear Daniel, my deepest condolences.

Your relentless efforts to help your wife are an overwhelming
testament to your love and devotion for her. We on the thread have been
first hand witnesses to your driving determination to find that next
step forward that would finally lead to the answer. Year after year
after year, your focus has never wavered. Together with your guidance
the entire thread has sought out that next step.

While that next step was beyond reach, you can be rest assured that
your wife has lit the way forward for others on this same journey. Of
all the tens of millions of people who have struggled with this illness,
 she was one of the few who did not stay on the well worn and known to
be futile trail, but instead made a path for herself. There can be
absolutely no doubt that we would be so much closer to finding that
final piece of the puzzle if more had also tried to blaze a new trail.

There is now an eternal light that guide the way for future fellow travellers.

Sincerely,                                                                              Jcancom

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Sat Oct 29, 2016 09:09 AM
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Daniel I am so sorry for your loss. I know when I
lost my mother back in February I was mad at the doctors that took care
of her because they just said go home and die with family. I moved her
in with my family and we set up hospice. It was not easy to watch.
I found this website all too late but keep reading the posts daily.
Your, finder, jcancom, and others. You all have something brilliant to
bring forth and I just wonder why you all have not gotten together to
really find a pathway that would help others. There are many on the blog
 that I am sure would contribute should you all ever decide to follow
that road. Not even sure if you all live in the same country but I think
 with your intelligence and your post, you can all come up with
something.
Love and light to all.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by MnMKona on Mon Oct 31, 2016 05:53 AM
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Oh man....me saying that I know how this feels
(sister & mother lost, father & wife, mother & father in law
 still fighting) is of little value.
But others have said it very well. This is such sorrowful news.
May the serenity prayer find it's appropriate way into your heart Daniel.

God, grant me the serenity to accept the things I cannot change,
Courage to change the things I can,
And wisdom to know the difference.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Danielus on Tue Nov 01, 2016 03:36 PM
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Dear Friends, thank you so much
 for all the beautiful kind and supportive thoughts and words. It means
so much to me ... I agree with Chris: we need to think how, together, we
 can take what we have learned to a next level.

What I haven’t wrote on my website is that until
one and a half month ago my wife was fine. Just before that we were on
holiday. But at the mid of September she had some constipation and she
went to the hospital to see if they can help. We stayed in the hospital
for 4 days and after that she went out of the hospital with edema and
coughing. The treatments used by the hospital have brought her in to a
state such that after that everything went out of control. Essentially,
they washed out of her blood all the minerals and other important
elements trying to lower Ca level which was elevated (but that was at
that level for the whole past year). Initially they did not want to
understand the origin of the high Ca in our case, as I explained: it was
 the elevated Cortisol produced by the tumor which in turn was leading
to a high para thyroid hormone, and which in turn was triggering loss of
 Ca from the bones. The right approach would be to focus either on
lowering Cortisol production or Phara Thyroid hormone. But their first
choice was to wash out her blood during many days … such a big mistake
for them doing it and for us accepting it. Finally, they understood they
 should focus on Cortisol or Para Thyroid but it was too late as they
already washed out her blood … it only took a few weeks after this to
lose my dear wife.

With this it becomes so clear to me that once they
have to deal with late stage cancer patients, most hospitals simply do
not know what to do. The protocols they have in place are for “easy”
jobs only.

As I wrote on my website, I intend to (very soon)
start up a Foundation to further help cancer patients and doctors with
anticancer treatment optionshttp://www.cancertreatmentsresearch.com/?p=1997

And that will not only be sharing info on my
website but also connecting doctors with patients and finding doctors
willing to start clinical trials with new drugs, in countries that allow
 for that. I am already working on that. But beyond that, I realize that
 cancer patients may need a structure not only for help with anticancer
treatment options but also with other treatments such as the one
encountered by us (calcium). Cancer patients have a sensitive body and
treatment protocols that work for non-cancer patients may not work for
cancer patients. Another challenge I found in our journey and that other
 friends have encountered is the time to diagnostic. If my dear wife
would be examined when her complains started, we would not be stage IV
at the diagnostic point. But the family doctor delayed the investigation
 by months so that we had to go to another country for a diagnostic.
Western Countries, at least western Europe, developed a system that it
aims at minimizing the costs (while insurance companies growing
profits). And cancer patients suffer the most from this. Either when
they should be diagnosed as time matters or when they are late stage
when hospital stop trying to help. Fortunately, there are countries
where costs are low and private clinics can enable a fast diagnostic. At
 some stage, via the Foundation, I also intend to address this aspect
and support patients to get a fast and low cost access to diagnostic
tools. So far, in Europe, I have a clear view on how to do that.

To conclude, I am in deep pain as I lost my dear
wife. Normally, I should not want to have to deal with cancer anymore.
But I know how painful can be all the steps from diagnostic until the
end, whether that is positive or negative, and I intend to try reduce or
 eliminate that pain for others. And if some of you agree we can think
how we can work together on that line. The most important is to make
effective solutions practical and accessible.

Thank you all for everything and it was a great
pleasure to meet you all on this forum as we grew our knowledge and
shared feelings together.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Meech90 on Wed Nov 02, 2016 10:01 AM
 Quote | Reply

It's scary to hear what one hospital visit can do. I
 was hospitalized for 3 days two weeks ago with kidney issues, and was
given a bolus dose of dextrose and a shot of insulin to fix a slightly
elevated potassium issue. I was worried at the time due to the dextrose
obviously feeding cancer cells and the IGF-1 receptors within the cancer
 cells. It's tough to be careful during emergencies and it's definitely
scary to hear what one hospital visit can actually bring.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by falconet on Wed Nov 02, 2016 12:50 PM
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My mom's condition also quickly deteriorated
after a Cataract surgery for which she was put into general anesthesia.
she immediately developed ascites and felt generally worse. she'd go for
 long walks till the surgery with no pain whatsoever and generally good
appetite. and then everything turned for worse. if it is a coincidence
and it has nothing to do with what happened to the hospital, it's one
hell of a coincidence.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Wed Nov 02, 2016 06:10 PM
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Cure heart disease with hypoxia? Suppose this is not great news for those oxygen bars that have sprung up.

https://www.sciencedaily.com/releases/2016/11/161101093856.h

Am trying to figure out how shutting down oxygen consumption might
actually help as an anti-cancer treatment. Seems to be going in the
wrong direction. Wouldn't this just push cancer cells to be more
glyocolytic? Sort of revving the Warburg effect. Might anyone help
out?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by MnMKona on Fri Nov 04, 2016 05:01 PM
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Take lots of time to get rested & centered
Daniel, we all know you must be totally exhausted physically,
mentally......& especially emotionally.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Meech90 on Fri Nov 04, 2016 09:38 PM
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Jcancom and finder, maybe you can chime in on this.
I've been looking at Gallium Scans. Essentially the same principle as
PET scans (injecting with a substance that is uptaken by cancerous
tissue vs injecting with a substance that is taken up by tissue
experiencing inflammation). Anyway, these scans are used to detect
cancer among other things. Has there been any thought given to combining
 this gallium with an anti-cancer substance, or possibly using a
substance which mimics gallium in order to attack cancer cells. It would
 be fairly specific, as it would be targeting areas of inflammation. Of
course it sounds like there would be some collateral damage, but I
believe the same can be said about substances attacking PET positive
tissue. Thoughts?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by ernemef on Sat Nov 05, 2016 12:05 AM
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Meech, you may find this interesting.

http://atlasofscience.org/a-novel-radionuclide-treatment/

a trial is presently underway in Melbourne and Sydney Australia.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Sat Nov 05, 2016 08:52 AM
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Dear Meech90,
Ga in itself is not specific for any tumor, as far as I know. It is in
its isotopic form, along with other elements, that is interesting. It
can be produced on-site from 68Ge(rmanium) and relatively easily
separated and coupled to the target molecule for tracing or treatment.
This is in contrast to 18F DG, which requires a cyclotron to produce.
And it is already in use, both for detection as well as for treatment.
kind regards, finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Sat Nov 05, 2016 09:38 AM
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I was under the impresssion that we need to increase glutathione in patients with cancer. Can someone comment on this?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Sat Nov 05, 2016 09:38 AM
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I was under the impresssion that we need to increase glutathione in patients with cancer. Can someone comment on this?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Sat Nov 05, 2016 09:53 AM
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I am trying to understand the
glutathione treatment. One post was for decreasing and another for
adding via IV. Can someone please clairify this for me.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Sat Nov 05, 2016 12:14 PM
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Are you saying they are using DMSO in an IV with 3BP????

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Sat Nov 05, 2016 04:56 PM
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Dear chris1023,
what is the purpose of your series of questions? Please expand on your motives.
kind regards, finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Meech90 on Mon Nov 07, 2016 04:54 PM
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Thank you to the two posters for their replies to my earlier post.

In other news, here is what Dr. Jason Williams is up to now: http://www.prnewswire.com/news-releases/doctor-develops-new-

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Mon Nov 07, 2016 05:31 PM
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I am just re-reading many of
the posts and find some contradicting posts. So just to be sure I want
to make sure I am educating myself correctly. SO if someone can comment
and claify, that would be great.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Mon Nov 07, 2016 05:41 PM
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Howard, just wondering how you are doing and what steps you have done?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Mon Nov 07, 2016 07:22 PM
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Dear chris1023,
I have a feeling that you are not being frank and honest with us when
you say that you want to educate yourself and want elucidation on
seemingly contradictory postings? But correct me if I am wrong about
this feeling. In that case, please explain what it is that you are
looking for, so we can try to be of help with your queeste. kind
regards, finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Mon Nov 07, 2016 07:27 PM
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Not sure what your getting at. The
posts I am reading, if you look back, ie DMSO in an IV or Orally, just
asked if that is really safe since DMSO is a solvent. We used to use it
with aspirin and rub it over painful joints. As far as Gluthione, I was
always under the assumption that GT is important to prevent cancer and
an instrumental antiox. but posts on here are saying to want to deplete
it. SO there are my questions.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Mon Nov 07, 2016 10:35 PM
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ernemef, Thank you for your post! It greatly cheered me up.

Clinical cancer research has more than its fair share of
disappointments: it is always encouraging to see treatments that
show some promise. The article that you posted at least on a first
reading seemed fairly positive.

The recent news about Novartis pulling away from CAR T-cells was a
tough one to cope with and then the phase 3 for peg argnine reporting a
negative result did not help any. These were multi-year efforts and it
is now not completely clear what their path forward might be. With
metabolic treatments, one has this dismal thought that they might never
be proven to be effective (nor not effective).

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by ernemef on Tue Nov 08, 2016 05:55 AM
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jancom here is a bit more detail on the LuPsma nuclear treat ment running in Australia:https://twitter.com/jillmargo1

Click on "More places may open for 'game-changing' nuclear treatment for prostate cancer"

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Tue Nov 08, 2016 05:58 PM
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Dear chris1023,
DMSO is a solvent. Glutathionine is important for cancer prevention, but
 many tumors have a high production of glutathionine, since it is
involved in cellular detoxification. It renders a lot of tumors
resistant against many forms of chemo, including 3-bp. Several
publications have shown that many resistant tumors become vulnerable
when glutathionine production is inhibited, including some melanomas.
Paracetamol is such an inhibitor. I hope this helps to explain the
observed inconsistencies you noted. kind regards, finder.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Tue Nov 08, 2016 08:25 PM
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Ernemef, wow! Good one!

Those results look very strong indeed. I am not sure how such a large
 turn around in cancer burden could occur so fast without some sort of a
 TLS response. Wonder if the laws in Germany would allow patients to
receive this treatment now.

If anyone else has more good news, please by all means post to our thread.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Tue Nov 08, 2016 11:02 PM
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"the absence of MCTs (monocarboxylic acid transporters) in β-cells explains the pyruvate paradox"

"In all tissues other than in the pancreatic islet, the expression of MCT1 and LDH contributes to flexibility of metabolism"

http://www.biochemsoctrans.org/content/36/3/300.long

I do not remember the thread ever discussing this one before. It
would seem to have importance concerning treatment strategies for
pancreatic cancer.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Wed Nov 09, 2016 06:09 PM
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Jcancom, I can tell you for certain that
clinical trails cost lots of money. I do know for certain that Dr Ko
will be starting this up soon. I will be posting on here from time to
time the outcomes, once they begin.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Wed Nov 09, 2016 11:07 PM
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chris, it has been such a disappointment to me
that through all these years on the thread the entire clinical trial
process continues to be based on fundamental principles that have not,
will not and probably could never create the change we all want.

 Some efforts have been made to change some of the basic flaws,
though from what I can see these flaws remain. We need to create a
process whereby all that is needed is a great idea. Moving back to a
regulatory environment where only safety would need to be established
and perhaps patients could finance their own treatment in phase 1 could
help us out of this near complete suspended animation in development
of a large range of oncology treatments.

When ernemef recently posted about a targeted radioactive treatment
and the success it was having in the clinic I was quite surprised
because I had read about that approach years and years ago and believed
that it must not have worked out because I had not heard any recent
reports.

If we could simply set up the right basic structure everything could
take care of itself. Adam Smith's recipe for a well functioning ecomomy
is surprisingly brief: self-interest, limited government and free trade.
 A 2 or 3 point plan to modernize the clinical trial process would move
us so much closer to our goal than yet more elaborate and highly
expensive big science projects.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Thu Nov 10, 2016 08:47 PM
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Dear chris1023, your actions, reactions are quite
puzzling. With this last reply you suggest to know more about the plans
 of Dr Ko than we do? If so, please elaborate! But unless you are the
person I spoke with on the phone tonight for more than 1 hour, I assume
you are making things up? kind regards, finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by chris1023 on Fri Nov 11, 2016 11:39 AM
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Finder, I am finding your reply's and accusations quite upsetting and why you are so puzzled.

But no I have never spoken to you.

Yes, I probably do know more plans then you do.

If you would like to discuss this I would be happy to call you on the phone should you provide your name and number.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Sat Nov 12, 2016 02:00 PM
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Dear chris1023,
There is no need to feel accused. I am just being cautious and hesitant
to interact here with people who come, to my opinion, storming in with
detailed questions and unverifiable claims. As you may well know, since
the Bracht catastrophe, 3-BP is under scrutiny by the AG in Germany.
This has resulted in an increase of visits to sites discussing 3-BP by
journalists and a lot of other people. You should be able to understand
that under these circumstances, your questions do arouse some
hesitation, at least that is the case with me. And if you further seem
to imply that you do know things about a clinical trial in which Dr Ko
is involved, then I am puzzled. And curious, eager to learn more. And if
 you are provinding some good reasons, I will call you.
kind regards, finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Mon Nov 14, 2016 07:37 PM
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Dear chris1023, so far no reply to my post. I am
still waiting for your additional explanations on the Ko trial, and a
good reason for your agressive questions, and the total lack of feedback
 on my explanations to your previous supposed questions. You are not
doing a good job for building a base of confidence with me. It is up to
you to prove my wrong. kind regards, finder.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Mon Nov 14, 2016 08:19 PM
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Dear finder, chris might be as frustrated as the
rest of us to get our thread back on track. It's been months and months.
 Is there any word about the investigation into Bracht? The issue about
how Germany might want to comprehenively modify its alternative medicine
 system clearly will be a lengthy political process, though the specific
 question of what happened at the Bracht clinic should, one would think,
 now have answers. Our thread is very much interested in and looking
forward to these answers.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Tue Nov 15, 2016 08:04 PM
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Always great to hear good news. Little bit unsure
 whether the good news is somehow entangled with the insider trading
allegations.

http://www.kurzweilai.net/dramatic-remissions-in-blood-cance

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Wed Nov 16, 2016 04:10 PM
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Dear Jcancom,
I am also frustrated by the disaster in Germany. But I am working very
hard behind the screens to try to get some positive things out of it. I
have probably paraphrased Rahm Emananuel here: "Never let a good crisis
go to waste". The extra attention Bracht has caused in the media, has
had several results so far, as judging from the MSm reporting. There is
more widespread attention to 3BP than before by cancerpatients. Some of
the relatives of deceased patients have comitted to the benefits of
3BP,despite the death of their relatives. This is in relation to the
fact that these patients were already given up, sent home to die.
Combined with the observation that the treatment with 3BP seemed to
improve the quality of live of these patients, it was sufficient for the
 relatives to declare this in a Dutch MSM journal. Furthermore, the
German legislation has so far not ruled against the system of "Natur
Heiler". The exact rules for 3BP application are under revision, but the
 essence of German law still allows to use 3BP at the discretion and
professional responsability of the doctor involved.
So far some of the facts. I can do a lot of speculation, but I rather
refrain from that for the moment.
But I stay with my remarks to chris: if you come up with claims, ask
questions, try to be open about your goals. It is reassuring to know
that a person is of good intentions. I hope I still have that impression
 from the other posters at this thread. Kind regards, finder.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Tue Nov 22, 2016 08:13 PM
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Dear Finder: It has been really tough waiting
this one out. Is there anything yet from the authorities on the
investigation? Do you have any urls from the post above from Dutch
media? I would love to hear some of the comments from the families who
thought that there was at least a symptomatic benefit.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Sat Nov 26, 2016 12:52 PM
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Dear Jcancom,
some links to articles in Dutch media:
http://www.omroepgelderland.nl/nieuws/2115593/Apeldoornse-na
http://www.volkskrant.nl/buitenland/oud-patienten-geloven-ni
http://www.trouw.nl/tr/nl/4516/Gezondheid/article/detail/436
http://www.rtlnieuws.nl/gezondheid/patient-omstreden-natuurg
All in Dutch, but Google can help you. At the moment, there is almost
perfect silence in the media. I will report if things do change.
kind regards, finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Nov 27, 2016 02:18 AM
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Dear finder, thank you for those links. Those
articles were very different in tone then almost all of the English
language commentary that I have read online. I am not sure how one can
go about breaking out of the anglo-Google world to the deutch-Google
one.

Came across this online. Sounds interesting. Are they really saying
that they could specifically target cancer cells with 3-BP using CXCL12.
 Could be extremely powerful if true. Comments please!

treating metastatic cancers. Cancer cells are sequestered and
specifically targeted using CXCL12, a potent chemoattractant, and
3-bromopyruvate, a glycolytic inhibitor.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by peggyznd on Sun Nov 27, 2016 01:38 PM
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Do you have a link to this article. I cannot find the exact article, so wonder if there is a corrected title or actual link.

Peggy

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Nov 27, 2016 04:13 PM
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Peggy, you posting to me?

I see what others meant how it can sometimes get a little confusing knowing who is posting to whom.

Looks like the kids might be ready to show us how its done with 3-BP.
 Everyone with a basic level of biology who reads our thread at least
has to admit that there is a whole lot of horsepower under the hood of
the 3-BP rocketship. If the kids at the science fair can find a way of
uncorking the potential it would not be hard to imagine massive
responses.

Any approach that could find a way to specifically target 3-BP to
cancer cells should have extreme anti-cancer effects. I am quite vague
though about CXCL12. About all I can guess is 3-BP bonded to CXCL12
which hones to the cancer vasculature.

Anyone be in the North Jersey area sometime around March of next
year. Wonder if someone biotech recruiters will be paying attention.

http://www.njrsf.org/

http://www.njrsf.org/ cgi-bin/CatList.pl

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Nov 27, 2016 05:05 PM
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I have no clue at all where the CXCL12 fits in
with 3-BP, though the below definitely seems quite interesting. Is it
really possible to have a chemotaxic drug act as a chemical robot? That
would be very cool! Any help figuring this out would be greatly
appreciated.

"It has also been shown that CXCL12 signalling regulates the
expression of CD20 on B cells.[16]CXCL12 is also chemotactic for
mesenchymal stem cellsand is expressed in the area of inflammatory bone
destruction, where it mediates their suppressive effect on
osteoclastogenesis"

For instance, blockingCXCR4, the receptor for CXCL12,
withPlerixafor(AMD-3100) increased the effectiveness of combretastatin
in a mouse model of breast cancer, presumably by preventing macrophages
from being recruited to tumours.[15][16]Furthermore, CXCL12 signaling in
 conjunction with CXCR7 signaling has been implicated in the progression
 of pancreatic cancer.[5]In the urinary tract system, methylation of the
 CXCL12 promoter and expression of PD-L1 may be powerful prognostic
biomarkers for biochemical recurrence in prostate carcinoma patients
after radical prostatectomy, and further studies are ongoing to confirm
if CXCL12 methylation may aid in active surveillance strategies.[25]In
the field of oncology, melanoma associated fibroblasts are stimulated by
 stimulation of the A2B adenosine receptor followed by stimulation of
fibroblast growth factor and increased expression of CXCL12"

https://en.wikipedia.org/wiki/Stromal\_cell-derived\_factor\_1

*"Chemical robots* that use artificial chemotaxis to
navigate autonomously have been designed. Applications include targeted
delivery of drugs in the body."

https://en.wikipedia.org/wiki/Chemotaxis

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by peggyznd on Sun Nov 27, 2016 07:21 PM
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Is there some one in this science fair whose work
 centers on 3-BP?  Still don't find a good link, but maybe it is my
search engine.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Nov 27, 2016 08:09 PM
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Yes. The link that I posted included the entry about 3-BP.

Here it is again.

Anything that could direct 3-BP directly to cancer cells obviously could be extremely potent.

http://www.njrsf.org/cgi-bin/CatList.pl

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Nov 27, 2016 08:28 PM
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Dear finder, could you help me out? Where might
they be going with CXCL12 and 3-BP? Big mystery, to me. Would it be
preconditioning the tumor area with something like Plerixafor as noted
on the wiki page and then using free 3-BP that is somehow attracted to
the altered CXCL12 cells? No idea, on this one.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by peggyznd on Sun Nov 27, 2016 08:49 PM
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Let us know when these are reviewed by the judges and that will be helpful.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Nov 27, 2016 11:30 PM
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I am not seeing the Saudi Journal of Medicine and Medical Sciences in pubmed. Article covered the 3-BP terrain quite well.

Would have like to see 7-aminocarboxycoumarins  mentioned https://www.ncbi.nlm.nih.gov/pubmed/24672058 and perhaps methyglyoxal, though they might have been thought as off the trail.

A sobering quote near the end of the article:

"Being antagonized by reduced glutathione and N-acetyl cysteine, 3BP
can be considered as a safe chemotherapeutic agent. When signs of
overdosage, toxicity, or undesirable side effects develop after 3BP
administration, reduced glutathione or N-acetyl cysteine can be given
immediately as a rescue therapy."

This is not exactly news to those on the thread, though one certainly
 wonders whether the patients at the Bracht clinic might still be alive
if this advice had been followed. Treating with NAC instead of vitamin C
 might have helped. I am not sure whether even the hospitals who cared
for the Bracht patients treated with it. That is likely a question of
interest in the investigation. Perhaps part of the German investigation
should consider how 3-BP rescue treatment with NAC or
reduced glutathione should be understood to be standard of care.

http://www.sjmms.net/article.asp?issn=1658-631X;year=2017;vo

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Tue Nov 29, 2016 05:22 PM
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Dear Jcancom,
I am afraid I have to disagree. If 3-BP is formulated inappropriately,
it will start by damaging the blood cells and the lining of the
bloodvessels. This will result in blood cloths, finally obstructing the
major veins and other vessels, especially capillaries in the brain.
Within minutes resulting in damage and ultimately, death. NAC nor
glutathionin can save one, however fast given. It can only help to
supress a short pulse of 3BP in the bloodsystem, but the blood itself
can more than adequately cope with this under normal physiological
surcumstances. kind regards, finder.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Danielus on Tue Nov 29, 2016 05:54 PM
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Dear Friends, based on personal
 experience, as seen on CT, ALA IV given 24h after 3BP injection in the
tumor, succeeded to help protect the tumor against 3BP action. That was
not the case when ALA was not given. Based on this, I expect that it
depends on how high the overdose is. If that is not too high, strong
antioxidants given IV may help the normal cells survive. If the dose is
way too high, there may be nothing to save.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Tue Nov 29, 2016 06:33 PM
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Dear Danielus,
glad to hear from you! We hope all is well with you, which is a strange
wish given the circumstances. But you will, we hope appreciate our
support for your cause. As for antioxidants in combination with 3BP, I
think there are several reports in scientific literature that show that
3BP can be counteracted with NAC, glutathionine. In general,
antioxidants do supress secondary effects of 3BP, whereas NAC directly
interacts with 3BP on a molecular basis. kind regards, finder.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Tue Nov 29, 2016 06:33 PM
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D, thanks for stepping in on this one. When I
read the Saudi article it occurred to me this is a pivotally important
question related to the Bracht incident. It was apparently understood
very early on that something was going south at Bracht. Administering
the rescue treatment at that time might have been helpful. I wondered
if the vitamin C that was given might have been of some help. It seems
doubly tragic that there might have been an effective rescue for Bracht,
 though it was unrecognized. I will be very interested to see how the
investigation considers this aspect of the Bracht tragedy.

We talked about the reduced gluthione and the patent related to 3-BP
treatment (?). Might look that one up again and see what it said. I am
glad that you mentioned the distinction between rescuing normal cells
and cancer cells. If TLS were the problem, then stopping the 3-BP effect
 would be important. If it were more about an overdose that was
affecting normal cells, then the question really is how strong of a
rescue effect there would be. I can see what Finder means about rescue
therapy probably not being too helpful once for example brain ischemia
has been ongoing for a while. The Bracht patients were reported to have
these stroke like signs. Rescuing patients at that stage might be
difficult.

D, have you had experience with reduced GSH when it helped rescue
normal cells more so than cancer cells? I think I remember you
mentioning how you had a bottle of reduced GSH on hand for safety.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Tue Nov 29, 2016 06:52 PM
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Dear Jcancom,
I can not imagine a toxic effect of 3BP which can not be countered by a
healthy body, if acute problems are avoided. 3BP is an eager molecule,
trying to react with exposed SH -groups on proteins and small molecules
like NAC and glutathionine. This is its primary way of working. But it
has a number of additional properties, making it suitable for cancer
killing. It is readily transported by MCT1, overexpressed in many solid
tumors. The combination of specifically reacting with enzymes involved
in glycolysis, being transported by MCT 1, and releasing Ca ions from
damaged mitochondria (thank you, Jcancom for the hint!), makes 3BP a
type of medication without any serious competitor, at this time of
writing. Regardless the (assumed) enormous input from big pharma to come
 up with an alternative. Until now, 3bp sets the level at which big
pharma will have to play. Eventually, they will succeed. kind regards,
finder.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Dec 03, 2016 12:12 AM
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Here's a patent on reduced glutathione. This
might have been the one we discussed on the thread earlier. I had not
remembered that this patent claimed that reduced glutathione could
actually have anti-cancer treatment effects.

https://www.google.ca/patents/WO2013103840A1?cl=en&dq=glutathione+3-Bromopyruvate&hl=en&sa=X&ved=0ahUKEwjy3euojdfQAhVD54MKHc7mChMQ6AEIHDAA

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by dumbcritic on Mon Dec 05, 2016 05:39 PM
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From Krebs to clinic: glutamine metabolism to cancer therapy
http://www.nature.com/nrc/journal/v16/n10/full/nrc.2016.71.h

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Tue Dec 06, 2016 06:50 PM
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Dear dumbcritic,
thanks for the link. It remains however, a tantalizing question how one
can discuss metabolic changes in tumours without even mentioning the
Warburg effect in relation to 3BP? And I just read another review on
metabolic properties of cancer, focussing on the Warburg effect, without
 even mentioning the impressive work of Pedersen and Ko? What is going
on? kind regards, finder

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Dec 17, 2016 08:55 PM
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I do not remember whether or not the thread has
talked about the below research before, though I am impressed. This
would seem to be an ideal combo for 3-BP. There are not that many escape
 routes open to cancer cells upon 3-BP treatment though the oxygenation
status surrounding the tumor would be one of the more obvious ones. Very
 smart idea using an anerobic microbe to attack cancer.

http://www.dailymail.co.uk/health/article-2033871/Soil-bacte

A while back I received a brown envelope which showed the history of a
 patient treated with 3-BP. Initially the patient had a very strong
response, though once the hypoxic region of the tumor was unveiled 3-BP
lost effectiveness. Having an anerobic therapy might help capture more
of 3-BPs potential. Perhaps it might be necessary to do some tinkering
though because from what I understand the url above requires direct
tumor injection. Might there be a way to package such a therapy which
could be given systemically?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Danielus on Sat Dec 17, 2016 09:35 PM
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Dear J,

More than a year ago, after connecting various pieces of information
and type of response to 3BP in patients, I came up with the idea that
there may be reponse to 3BP only in the regions of the tumor exposed to
oxygen http://www.cancertreatmentsresearch.com/?p=125.
 When that idea came to my mind it was an AHA moment and I still belive
in it. But that was just a theory, and I do not have yet evidence to
support that. Should I understand that you now have evidence to support
that in the "brown envelope"? If yes, can you please share more on the
evidence?

A little more about treatments with Clostridium http://www.cancertreatmentsresearch.com/?p=1207.

Kind regards,

Daniel

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Dec 17, 2016 10:17 PM
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There D, this was from a patient we looked at off
 thread from an email of 8-18-2015. The first CT was dated 5/2011. The
patient was nomically described as stage 4 bladder.

The patient had a truly monumental cancer burden. Liver, pancreas, prostate...  Pretty much the entire mid-chest.

3-BP initially seemed to have short term effectiveness. After a month
 of treatment: pancreatic lesion no longer visible, pelvic lesion no
longer visible, sacral mass no longer visible. Yet, there remained a
huge amount of tumor. Tumor did not respond after the first round and
became resistent. Tumor only appeared to be responsive for about a
month.

Those looking for the downside would say well that really has
no clinical relevance. The patient would receive no sustained benefit,
and likely achieve zero increase in life expectancy. That would be true,
 though cancer is typically like that, it wouldn't be defeated with just
 one victory.

The ominous implication also is that 3-BP might in some circumstances
 simply potentiate the hypoxic tumor mass to express resistance.

There are not that many metabolic escape routes available to a cancer
 cell with 3-BP. It would be very interesting to see what might happen
if there were combo 3-BP and anaerobic treatment.

As a note, the above referenced patient was treated with

TACE; not clear if there was one treatment or more over a period of
one week. The conclusion noted that more dosing would be necessary. Also
 larger tumore simply would not receive adequate 3-BP. This is somewhat
strange because there are other largish 3-BP TACE responses out there
online, so it seems quite reasonable to expect that this patient would
have done better if more than a week of treatment had been given.

D, do you read this patient report in the same way? Is this a question of induced resistance due to uncovering hypoxia?

The biological rationale being that MCT expression is driven by oxygen levels.

All the best of the season to you, D, and to all of our friends on the thread!

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Dec 17, 2016 10:25 PM
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A lot of the time I feel that I am a step or two
behind the researchers when they think up a novel approach to cancer
therapy. Targeting hypoxic regions with anaerobic bacteria is very
smart. The articles describe how this treatment has a surgical
precision. The bacteria can only survive in an environment without
oxygen.

Research was also out of Hopkins.

A combo with 3-BP would appear to be a very helpful strategy.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Dec 17, 2016 10:49 PM
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D, something that I also found interesting in
reading about this was that such bacteria might be behind the large
anti-tumor responses reporte by Colley many years ago.

I also found it interesting that one article suggested that anerobic
bacteria might now become used in alternative cancer treatment. Is this
legal? Could alternative medicine actually use bacteria found in dirt as
 a treatment?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sat Dec 17, 2016 11:34 PM
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D, should have consulted your webpage. You were
all over the Clostridium story and have a great write-up for it. Some of
 the media reports were using the phrases "cancer cure" applicable to a
wide range of tumor types. What would cancer be like if there were no
areas of hypoxia? Would cancer then be an illness that could be truly
considered a manageable chronic disease? Cancer is so protean, it never
appears to have an attackable weakness that is always present: hypoxic
regions would be one such weakness.These
 hypoxic regions could be especially dangerous because they occur where
the blood vessels have grown well beyond their supply lines. They would
be the deep seated tumor masses.

Perhaps hypoxic regions would be less vulnerable to apoptosis and
free radicals becasue their mitochondria would not be capable of
functioning without oxygen.

D, do you also see the substantial potential of a combo 3-BP and anaerobic treatment?

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Dec 18, 2016 12:10 AM
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3-BP enters through simple diffusion. Is that a new one?

"one of the most extensively studied anticancer compounds is
3-bromopyruvate [89]. It is well known that 3BP enters cancer cells not
only by speci?c MCTs but also by simple diffusion "

free full text

http://www.mdpi.com/1420-3049/21/12/1730

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Dec 18, 2016 07:31 PM
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Sorry everyone I am just so excited by these Clostridium bacteria that I can't let go of them!

The research that I have read suggests that these bacteria can create
 molecular scale margins that are not possible surgically. The bacteria
will destroy the hypoxic cells right up to the normoxic cells. Any
cancer therapy that is that specific should have very significant
treatment effects. Research has also mentioned that the genetic
engineering of Clostridium really has only started with novyi NT. Any of
 its genes could modified or any other gene could be added in. We are
only getting started with this and D's references show that there have
already been patient responses.

I would love to see what might happen if they were to do a
Clostridium oncolytic virus combo. Perhaps the endospores could be
invected with the virus. When the endospore opened up inside the hypoxic
 zone the virus could start to multiply. The great part of this is that
the hypoxic zone is apparently immune privledged. What I understand that
 to mean is that immune cells would not have entry, thus the viruses
could replicate without being attacked by the immune system. Once the
bacteria had cleared all of the hypoxic cancer cells, the pool of virus
would then be able to infect and lyze cancer cells in the normoxic zone.

I'd also love to see what would happen if the bacteria were
genetically engineered to be luminescent. Photodynamic therapy could
then be used. Here again the great part is that once all the hypoxic
cancer cells had been eliminated the bacterial light source would be
right on the margin between hypoxia and normoxia. This might allow for a
 treatment effect perhaps 1 cm beyond where the bacteria would be
effective. There might even be a way to amplify the luminescent effect
using radiation or a chemical introduced into the treatment enviroment.

Some of the other research noted that apparently hypoxic regions can
actually occur in quite small tumors. It would be such an amazing chance
 to enter the tumor at the centre and attack from the inside out. Most
approaches start on the outside and work in, this is known to be
hopelessly ineffective because the hypoxic zone does not have the
environment necessary for cancer cell lysis. Up till this point the
outside in therapies can be fairly effective, though the remaining cells
 that are not destroyed then cause tumor rebound. Removing the inside
and beyond with bacteria etc. could be a very effective approach.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by finder on Sun Dec 18, 2016 08:28 PM
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Dear Jcancom,
please do not get too overexcited by the Clostridium, Candida might be an other source. I personally do not accept these theories.What
 is factual,is that many lipopolysaccharides fom the glycocalyx of
several bacteria, are able to induce the conversion of monocytes into
macrophages. And macrophages are involved in the innate immne system.
And this is really complicated. kind regrads, finder.

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### RE: Anyone used 3bp (3-bromopyruvate)?

 by Jcancom on Sun Dec 18, 2016 09:52 PM
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Dear Finder, yes my previous enthusiasms have
been nearly entirely shown subsequently, as evidenced by our thread, to
have been misplaced. Notwithstanding, I still will call it (somewhat
hesitantly) partypooping, though with cancer fair enough if you can
see daylight odds are slim that you will actual reach that daylight
before that 6'5 300 pound linebacker makes contact.

I am really interested to see what the translational scientists will
add onto Clostridium treatment. In one of the studies they found there
was large responses in 30% of mice. Might probiotics up that number as
it did with ipilimumab? What genetic changes might be helpful? For
example might the bacteria start making paracetamol or 3-BP? There is a
whole lot of road out there and it will be very interesting to see how
they will amp up Clostri.

I am especially excited about the potential to actually meaningfully
redefine what cancer is. Eliminating hypoxic zones would clearly do
that. There is self-apparent strategy that cancer could use to counter
this effect. For all the research that has been done, nothing that I am
aware of has actually fundamentally change the essence of cancer in such
 a way.

On D's site, one of the posters wanted to know whether alternative
clinics are offering this. Some of the websites were also suggesting
that this might move to alternative clinics. I found this surprising.
Apparently, genetically modified engineered organisms as medicine are
required to not only receive FDA approval but also approval from a
branch of the NIH. Would it really be legal for an alternative clinic to
 find some high school kids to CRISPeR up some Clostri? Considering that
 a phase 1 for these bacteria has already been published this might be
legal under Right to Try.

Best Wishes of the season to you, Finder.

I am sure all of us on the thread are looking forward to a Great Year for 3-BP in 2017.

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