Tutorial II

https://youtu.be/0agjJZzORZE

The second tutorial of the game.

## Introduction the to Genome Editor

An introduction to the genome editor so it can teach you how it works.

## Estimated time

40 minutes to 1.3 hours

This tuorial will guide you through how to use the genome editor. Please start by going to thr geome editing lab.

### Step 2

Overview

Good!

What you see here is the genome editor. It is divided in two parts:

- The preview window

- The mode editor

We will start off by introducing the preview window. It shows you your organism at a certain age. The slider right below the preview is used to set at what age you whould like to view your organism. Now set the preview to show your organism at age 2.0 h.

#### Solution:

Press the slider and scroll it until it shows 2.0 h age.

### Step 3

Good job!

That is all we need to know about the preview for now. Now some genetics! All cells in the human body have the same DNA, but muscle and brain cells behave very differently. This is reffered to as diffrent modes of a cell in our lab. The genome editor can handle 40 different modes. Each mode has it's own properties. One mode is edited at a time in the genome editor and to change what modeis currently being edited we use the selector "Edit mode"

Now let's edit the mode called M5.

#### Solution:

Select M5 in the edit mode by clicking on it.

### Step 4

Inital mode

Now all properties you see below the selector you just used describe properties of mode M5. You might wonder, what mode are the cells in the preview in? They are in mode M1 because M1 is by default the inital mode. What mode is inital just tells the preview what mode to start with. Now make M5 inital instead by pressing the radio button "Inital"

### Step 5

Nice work! There are a lot of diffrent properties in each mode, more than we can go through in this tutorial. This mean you will have to explore them yourself. If you want more information about any setting in the genome editor then just click on the accompaying text with an underline. Now read the text about the property "Red color". You might have to scroll down to see it.

### Step 6

Finding Properties

Good job! Now find two more properties and make your mode M5 cells completely red.

### Step 7

Very beautiful!

If you drag the preview time slider back and forth you see your cells dividing. Many settings control how the divisons takes place.

Two important settings are what modes the two daughter cells are in. These settings are labeled "Child 1: mode" and "Child 2: mode". Set them so that when a mode M5 cell splits:

- Child 1 is of mode M6

- Child 2 is of mode M7

### Step 8

Good job!

You should now see two diffrent cells, one in M6 and one in M7.

Increase the preview time to 4 h to see what these cells split into.

### Step 9:

Close the circle

Good job!

You should now see 4 cells. Two in mode M6 on the left and two on mode M7 on the right. The family tree looks like:

time: 0.0h

/ \

6 7 time: 2.0 h

/ \ / \

6 6 7 7 time: 4.0 h

Now change the family tree into

5

/ \

6 7

/ \ / \

5 5 5 5

by setting the "Child 1: mode=M5 and Child 2: mode=m5 of mode M6 and M7

Tip: Instead of changing the selector "Edit mode", you can tap on cells in the preview to change what mode to edit.

### Step 10:

Insert into Microscope

Good job! Now your inital M5 cell splits into an M6 and M7 cell. They will further split into two M5 cells each and the life cycle is complete. Now place one of these cells in the microcope and see how three types of cells, but all with the same genomes.

## Description

Follow the instruction in the pop-up dialogs.