recursal/Fanatic-Fandom huggingface dataset:
Worm Genomic Prep

## Reagents

* Lysis Buffer
	+ 200mM NaCl
	+ 100mM Tris-HCl pH 8.5
	+ 50mM EDTA, pH 8.0 0.5% SDS
	+ -add Proteinase K to 0.2mg/mL before use (stored as 20mg/ml stock in -20°)

* phenol/chloroform/isoamyl alcohol
* chloroform/isoamyl alcohol
* 3M Sodium acetate
* 100% ethanol
* 70% ethanol
* TE
	+ 10mM Tris-HCl, pH 8.0
	+ 1mM EDTA, pH 8.0

## Protocol

1. Pick N2 adult hermaphrodites onto 20 plates, 10 adults per plate, and place at 20°.

2. Wait five days until most of animals on plates are gravid adults

3. Bleach worms (see accompanying protocol) and put carcasses and eggs on 7 plates with no food, place at 20°.

4. Wait two days.

5. Rinse the worms off each plate with 2mls of M9 media and put them in 15 ml conical tubes.

6. Pellet them by spinning 2 minutes at 3000RPM in clinical centrifuge.

7. Remove supernatant and add 5 volumes of lysis buffer with Proteinase K (~100µL pellet of worms is convenient).

8. Incubate at 65°C for 1-2hrs.

9. Incubate at 95°C for 20-30min.

10. Add DNAse-free RNAse to 0.1mg/mL and incubate at 37°C 1 hour.

11. Extract twice with 1 volume phenol/chloroform/isolamyl alcohol.

12. Extract once with chloroform/isoamyl alcohol.

13. Add 0.1 volume 3M sodium acetate and >2 volumes 100% ethanol.

14. Mix and incubate at RT or in the freezer for at least 1hr.

15. Pellet DNA by centrifugation at 14000rpm for 15min.

16. Remove supernatant and wash pellet with 70% ethanol.

17. Air dry and resuspend in 200ul TE. Determine concentration and dilute if necessary.
Ethanol Precipitation of DNA

## Reagents Needed

* 3 M sodium acetate pH 5.2 or 5 M ammonium acetate
* DNA
* 100% ethanol

## Protocol

1. Measure the volume of the DNA sample.
2. Add 1/10 volume of sodium acetate, pH 5.2, (final concentration of 0.3 M)
	* These amounts assume that the DNA is in TE only; if DNA is in a solution containing salt, adjust salt accordingly to achieve the correct final concentration.
3. Mix well.
4. Add 2 to 2.5 volumes of cold 100% ethanol (calculated after salt addition).
5. Mix well.
6. Place on ice or at -20 degrees C for >20 minutes
7. Spin a maximum speed in a microfuge 10-15 min.
8. Carefully decant supernatant.
9. Add 1 ml 70% ethanol. Mix. Spin briefly. Carefully decant supernatant.
10. Air dry or briefly vacuum dry pellet.
11. Resuspend pellet in the appropriate volume of TE or water.
SOC Media

1. To 800 ml distilled H2O add:
	* 20 g Bacto Tryptone
	* 5 g Yeast Extract
	* 0.5 g NaCl
	* 833 uL 3M KCl
	* 10 mls MgCl2
	* 10 mls MgSO4
2. Adjust final volume to 1 L and mix
3. Divide into ten 100 ml aliquots and label bottles
4. Autoclave for sterilization
5. Allow to cool
6. Aseptically add 2 mls sterile 1M glucose to each aliquot
900 Dias de Namoro

***900 Dias de Namoro***é um curta-metragem de animação brasileiro, produzido pela Lincoln Animation e lançado originalmente em Junho de 2017. É o sexto curta de animação da Lincoln's e o terceiro e último da série Cheshire Cat. A produção foi a segunda animação sonora da Reforma Linck, e ajudou a impulsionar definitivamente a dublagem nas animações da Lincoln's. Foi baseado em uma tirinha publicada no Facebook das Crônicas de Wesley. Neste curta ouvimos pela primeira vez a voz dublada de Scarlett O'Cat.
Batata

***Batata*** é um curta-metragem híbrido de animação e live-action brasileiro, produzido pela Lincoln Studios-Film e lançado originalmente em 23 de Dezembro de 2019. É o quinto curta em live-action da Lincoln's e o primeiro curta em live-action sem cunho religioso do estúdio. O curta é adaptado de uma crônica de Luís Fernando Veríssimo, que parodia uma discussão filosófica a respeito de um dito popular do Brasil.
Chemically Competent Cells

1. Pick a single bacterial colony and start a 5 ml culture in LB. **There is no antibiotic resistance involved with these bacteria so sterile technique is important!**
2. Grow overnight at 37**°**.
3. Put the rotor, 0.6 ml Eppendorf tubes and your reagents (MgCl2-CaCl2 solution, 0.1 M CaCl2+ 10% glycerol) in the cold room.
4. Inoculate 200 mls LB with 2 mls overnight culture in a 2L flask and incubate at 37**°**, monitoring the growth of the culture by taking its OD600.
5. You will harvest the bacterial culture wen the OD600 reaches 0.35-0.4 and this may take several hours. **If it is higher than this, you cannot go forward with the rest of the protocol.**
6. When your culture is getting close, put four 50 ml falcon tubes on ice and turn on the centrifuge; setting the temperature to 4**°.**
7. Transfer the culture to the cold 50 ml falcon tubes.
8. Keep on ice for 10 minutes. **At this point it is very important that the cells are kept ice cold for the remainder of the protocol. ALWAYS keep them on ice!**
9. Spin the cells down at 4000 RPM for 10 minutes at 4**°**.
10. Decant the media carefully and stand the tubes upside down for 1 minute to get out as much of the media as possible.
11. Re-suspend each pellet by carefully swirling or **gently** vortexing in 15 mls of cold MgCl2-CaCl2 solution (60 mls total). Spin down the cells again.
12. Decant the media carefully and stand the tubes upside down for 1 minute to get out as much of the media as possible.
13. Re-suspend the pellet by carefully swirling or **gently** vortexing in 1 ml (4 mls total) ice cold 0.1 M CaCl2+ 10% glycerol.
14. Aliquot 50 uL/0.6 ml tube in the cold room and freeze in liquid nitrogen.
15. Store at -80**°**.
Live Microscopy (C. elegans Embryos)

**Protocol**

1. Prepare 2% agarose pad.
2. Dissect embryos from 2-4 young adult worms (simply cut worms in center using 30G1/2 needles) into a 10uL drop of 1X Egg Buffer on 22x22mm coverslip.
3. Mount on 2% agarose pad, securing cover slip with small drops of 2% agarose.
4. Circle areas containing embryos on back of slide using sharpie.

**Deltavision settings:**

1. Switch to #5 dichroic filter (for GFP/mCherry tags)
2. Move UV filer into position (back of microscope)
3. Use 20X objective to find embryos, image with 100X objective
4. Z-stacks: 2micron
5. Timing: 20 sec intervals, total imaging 1 hour
6. Exposures: 0.05 sec (GFP-Membrane), 0.1sec (mCherry-H2B)
Guernica

Aryane Menin

Camila Macedo

***Guernica*** é um curta-metragem de animação brasileiro, produzido pela Lincoln Animation e lançado originalmente em Setembro de 2012. É o segundo curta de animação da Lincoln's e pode ser considerado seu primeiro documentário. Trata-se de um estudo da famosa pintura cubista de Pablo Picasso, que se inspirou na tragédia da guerra civil espanhola para produzir a tela "Guernica". Por originalmente ser um trabalho escolar de Lincoln e suas amigas, é o primeiro curta do estúdio a trazer uma narração sonora.
Bleaching Worms

## Full Plate

* Be sure you have lots of gravid adult hermaphrodites on the plate.
* Wash worms off plate with M9 media. Transfer to Eppendorf or conical tube depending on volume.
* Spin worms down 2000RPM for 2 minutes. Drain supernatant.
* Make fresh bleach solution: Mix equal volumes of 3:2 bleach:water and 1M NaOH. This must be made fresh the day you bleach.
* Add bleach to worms. Vortex.  Spin worms down and drain supernatant. Worms can be in bleach solution for maximum 4 minutes.
* Wash worms two times with M9 media (add M9, vortex, spin down and drain supernatant).
* Resuspend carcasses and eggs in some volume of M9 and pipet onto plate.  If planning on synchronizing worms, pipet onto plate with no food (worms will arrest as L1s).

## Spot Method

* Make fresh bleach solution: mix equal volumes of 3:2 bleach:water and 1M NaOH.
* Pipet 25ul onto plate off the square of bacteria.
* Pick 15-20 gravid adult hermaphrodites into drop.
* Next day, pick L1s that have crawled out of adult carcasses into bacteria onto a new plate.
Da Bíblia

***Da Bíblia***  é uma série cinematográfica produzida pela Lincoln's. É a segunda série com teor religioso do estúdio, sendo inspirada pela série "A Fé Nova-Apostólica". A série mostra as visões da Lincoln's e seus artistas para as histórias do Velho e Novo Testamento, que compõe a Bíblia cristã. Algumas histórias podem ser ambientadas na época de Cristo, ou períodos anteriores, até chegar nos dias atuais. Lincoln considera esta série como "a mais importante do estúdio", onde ele finalmente pôde mostrar a sua visão de mundo e também o que ele acredita sobre religião e Deus. Apesar da primeira temporada da série ter sido produzida com o Theatro INA, a série não teve influência ou financiamento da Igreja Nova Apostólica. Certos ensinamentos que Lincoln aprendeu durante sua vida como um novo-apostólico podem influenciar de alguma forma os roteiros da série.
Meu Querido Papai

***Meu Querido Papai*** é um curta-metragem de animação brasileiro, produzido pela Lincoln Animation e lançado originalmente em Setembro de 2014. É o terceiro curta de animação da Lincoln's e o primeiro da série Cheshire Cat. A produção foi a primeira lançada durante o período da Reforma Linck, e consolidou o estúdio de animação da Lincoln's. Foi baseado vagamente em tirinhas publicadas no Facebook e algumas ideias de Lincoln. Mostrou pela primeira vez uma paródia a um filme famoso: Star Wars, e estreou os personagens The Crazies pela primeira vez. É a primeira aparição em cores de Cheshire Linck e Eddie Linck.
PCR from Worm Lysate

**Single worm PCR:**

* Pick an individual worm into 5ul 1X PCR buffer + 200ug/ml Proteinase K
* Freeze at -20° or -80° for at least 30 minutes
* Run lysis protocol on PCR machine: 1 hour 65° 15 minutes 95°
* HOLD at 15°

**To each 5ul worm lysate add:**

* 2ul 10X PCR buffer
* 2.5ul 2mM dNTPs
* .25ul TAQ
* 1.5ul 10mM primers
* dH20 to 25 ul

*This lysate can also be split, 2.5ul per PCR reaction.*

**Run on PCR program:**

* 20 sec 95°
* 30 sec 95° (denaturing step)
* 30 sec Tm-5° (annealing step)
* 72° 1 min/kb of product (elongation step)

*The above is 35X*

* 72° 10 min
* HOLD at 15°

**PCR on population of worms:**

* Pick 5-10 L4 or adult worms into 15ul 1X PCR buffer + 200ug/ml Proteinase K
* Freeze at -20° or -80° for at least 30 minutes.
* Run lysis protocol on PCR machine:
	+ 1 hour 65°
	+ 15 minutes 95°
	+ HOLD at 15°
* Set up PCR reactions:
	+ 2.5ul 10X PCR buffer
	+ 2.5ul 2mM dNTPs
	+ .25ul TAQ
	+ 1.5ul 10mM primers
	+ 1ul worm lysate
	+ dH20 to 25 ul

*Use same PCR program as above.*
 Tm= (# of Gs and Cs in primer)\*4+ (# of As and Ts in primer)\*2

Ideal Tm of a primer = 60°

**10X PCR Buffer**

* 100mM Tris-HCl, pH 8.3
* 500mM KCl
* 15mM MgCl2

**Troubleshooting**

if your PCRs are not working, test several things:

1. test your lysates against primers that work. the issue may be your lysate or your primers and this expmt allows you to test the lysate with primers that have worked.
2. amount of template: although 1ul of worm lysate made from a population of worms usually works, you can also test 2.5ul and 5ul to see if that works better.
3. altering the Mg concentration can affect your PCR. redo your PCR with a range of Mg concentrations from 1-2mM (.25mM increments).
4. try a touchdown PCR:
	* 94° 30 sec
	* 10 cycles:
		+ 94° 30 sec 1o degree above the melting temperature of your primers 10 cycles and subtract 0.5° with every cycle (total of 5°; typically 61-56°) 30 sec
		+ 72° 1 min (the standard is 30 sec – 1 min/ kb of PCR product)
	* 25 cycles:
		+ 98° 30 sec 4° below the melting temperature of your primers 30 sec 25 cycles
		+ 72° 1 min (the standard is 30 sec – 1 min/ kb of PCR product)
	* 72° 10 min
	* Hold at 15°
5. some primers just don’t work, especially with such a dirty lysate. order some new ones and try them.
Lincoln Records

***A Lincoln Records*** é um estúdio de cinema amador fundado em 17 de Setembro de 2010. Durante algum tempo o nome representava a equipe responsável pela produção do som na Lincoln's. A Lincoln Records mantém e gerencia as trilhas sonoras e dublagens de todos os filmes dos estúdios, possuindo um canal do Youtube e um Soundcloud, onde disponibiliza tais arquivos.
Jesus Cristo está no Barco!

***Jesus Cristo está no Barco!***é um curta-metragem de animação brasileiro, produzido pela Lincoln Animation e lançado originalmente em 10 de Janeiro de 2015. É o quarto curta em animação da Lincoln's e o primeiro da série religiosa do estúdio A Fé Nova-Apostólica. É a primeira produção da Lincoln's apresentando um personagem falante, apesar de ser dublado pela voz do narrador da história. É também o primeiro curta animado sonoro da Reforma Linck. Foi adaptado de um escrito do Apóstolo Maior Jean-Luc Schneider, presidente internacional da Igreja Nova Apostólica na época. Apresentou vários personagens do The Crazies pela primeira vez. Estreou com este curta o novo logotipo da Lincoln's, na forma de Cheshire Linck segurando uma espada e uma câmera, ajoelhado diante de uma "janela" com a constelação do Cruzeiro do Sul atrás.
Histórias de Comunhão - 90 Anos INA Brasil

***Histórias de Comunhão - 90 Anos INA Brasil*** é um curta-metragem em live-action brasileiro, produzido pela Lincoln Studios-Film e lançado originalmente em Novembro de 2017. É o quarto curta em live-action da Lincoln's, e o terceiro da série A Fé Nova-Apostólica. Produzido para exibição em um evento do aniversário de 90 da Igreja Nova Apostólica no Brasil, o curta mostra depoimentos de membros da Igreja e suas memórias sobre a formação das comunidades no país, além de fotos e informações históricas da Igreja em São Paulo. Pode ser considerado, junto de Guernica, um dos poucos documentários realizados pela Lincoln's.
Lincoln Studios-Film

***A Lincoln Studios-Film***  é um estúdio de cinema amador fundado em 17 de Setembro de 2010. Originalmente este era o nome da companhia, sendo alterado em 2015 quando o nome passou a ser utilizado pelo estúdio de live-action, e o nome Lincoln's assumiu como nome geral da companhia. A equipe responsável pelo live-action esteve presente na Lincoln's desde a sua fundação, mas como um estúdio independente desde 2015, com o lançamento da primeira produção live, "Agradecer". A Lincoln Studios-Film produz principalmente filmes com fantoches. Também é responsável pela produção dos vídeos semanais das séries do canal da Lincoln's no YouTube.
O Primeiro Natal

***O Primeiro Natal*** é um curta-metragem híbrido de live-action e animação brasileiro, produzido pela Lincoln Studios-Film e lançado originalmente em Dezembro de 2016. Teve duas pré-estreias antes do lançamento oficial, realizadas na INA Santo André. É o segundo curta-metragem em live-action da Lincoln's e o primeiro da série Da Bíblia. É baseado nos relatos bíblicos do nascimento de Jesus Cristo. É o primeiro musical "verdadeiro" do estúdio, sendo sua primeira tentativa "Agradecer", no ano anterior. É um dos projetos mais retrabalhados de Lincoln, possuindo 4 versões diferentes: 2 trabalhos em andamento, 1 versão final e 1 edição especial para o Theatro INA.
O Galo e a Pérola

Manuque Glub

Julian Madagascar

6 minutos (Versão YouTube)

***O Galo e a Pérola*** é um curta-metragem de animação brasileiro, produzido pela Lincoln Animation e lançado originalmente em 07 de Setembro de 2011. É o primeiro curta de animação da Lincoln's, o primeiro curta da série "La Fontaine" e também o filme inaugural do estúdio. É baseado em uma das fábulas de Jean de La Fontaine, que critica a sociedade da França no final da Idade Moderna. Apresentou pela primeira vez o personagem central da Lincoln's, Cheshire Linck Cat, e até o momento sua única aparição canônica em seu visual de 2010.
Lincoln's

A ***Lincoln's*** é uma companhia amadora de cinema, que reúne os Estúdios Lincoln, que produzem anualmente animações e live-actions dos mais variados tipos. A Lincoln's foi fundada em 17 de Setembro de 2010 sob o nome "A Lincoln Studios-Film". Em 2015, com o início das atividades do estúdio live-action, o tal estúdio absorveu o nome original da companhia e um novo nome, Lincoln's, passou a representar toda a companhia. A Lincoln's tem presença nas redes sociais e também no YouTube. Lincoln's também é o nome oficial dos estúdios nos lançamentos, com sua fonte tipográfica aparecendo sobre os títulos das produções em pôsteres, miniaturas de vídeos no youtube e outros produtos vinculados.
Ellia Maya

**Ellia Maya** was an advisor to the Royal Family of Syhl Shallow and especially close to Nolla Verin. She also acted as a spy for the country, infiltrating syhl shallow and pretending to be on their side, while secretly working with Lilith.

## Biography

### A Vow So Bold and Deadly

Working as a spy against Sylh Shallow, Ellia Maya's true name was *Chesleigh Darington*

Her parents were killed by the former Queen of Sylhwhich was the story she told to explain why she was spying on them. It later turned out that this story was true. She then worked in the employ of the Grand Marshal of Wildthorne Valley and was brought to a party by Crown Prince Rhen in Ironrose Castle. After a meeting with him, Ellia Maya promised him information about Sylh Shallow's military movements and a weapon, which can be used against Magesmiths. She died in an ambush on a small group around Grey and Harper.

## Physical Description

Ellia Maya has waist-length dark hair, olive skin and calculating gray eyes. She has a scar on her cheek that stretches into her hairline over her ear, where the hair has grown back in a narrow white streak.

## Trivia

* Ellia Maya spoke both Syssalah and the language of Emberfall.
You went a walk¹ вики

## Добро пожаловать на **You went a walk¹ вики**

Игра созданная Vaydoez (https://gamejolt.com/games/ywaw1officalpage/464458)

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====================================================================ИГРЫ===============================================================

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Первая демо (для друзей) > CLICK ME!

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===================================================================ИСТОРИЯ/СМЫСЛ/СЮЖЕТ===========================================

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## Эта вики посвящена игре You went a walk¹ сделанная Vaydoez!

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**YWAW - пародия на игры 90х, 80х**

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O Filho Pródigo

***O Filho Pródigo*** é um curta-metragem em live-action brasileiro, produzido pela Lincoln Studios-Film e lançado originalmente em 05 de Agosto de 2017. Teve um pré-lançamento em 28 de Maio de 2017, em uma versão incompleta, durante um evento da celebração dos 90 anos da Igreja Nova Apostólica no Brasil. É a terceira produção em live da Lincoln's e o segundo curta da série Da Bíblia. É a primeira produção da Lincoln's em widescreen anamórfico. Baseado na história bíblica do Evangelho de Lucas, traz o relato da época de Cristo para uma época mais contemporânea, entre os séculos XVIII-XIX. Pode ser considerada a primeira produção em média-metragem do estúdio, devido a sua duração de quase 48 minutos.
Federation of Vichumiy

The **Federation of Vichumiy** is a "country" located inside Shavijan. It is one of the three countries in the 3 Countries plan.

## Dictator

The dictator of the Federation of Vichumiy is Salvador. The Federation of Vichumiy is a country that is part of Shavijan like Andyara but also a "independent" country like Andyara. This country is a country that is Colterlianist which is idea dictator Salvador.

## Colterlianism

Colterlianism is a adapted, perfect form of Communism. Colterlianism is where every body is free and will not answer to any boss at work, but private properties . So far this is just Communism but this is where Salvador steps. The main problem with Communism is that nobody tells them to work so they don't. Then the government will take control and it will not be free. Salvador says that if you do work you will get paid and if you don't work you will not get paid(If you are sick your planned a vacation you will get half paid). This will lead to a ton of homeless people who didn't you want to work. The government will take the homeless give them food, shelter, water but they have to do the bidding of government(If you are homeless because of family reason it is ok),you will not be able to escape.
Agradecer

***Agradecer*** é um curta-metragem híbrido de live-action e animação brasileiro, produzido pela Lincoln Studios-Film e lançado originalmente em Outubro de 2015. É o primeiro curta em live-action da Lincoln's, e o primeiro curta em live-action com cunho religioso do estúdio. É o segundo curta da série A Fé Nova-Apostólica e foi baseado na história bíblica de Caim e Abel e no conceito de oferendar e agradecer da Igreja Nova Apostólica. Foi a primeira produção da Lincoln's a utilizar fantoches e atores reais.
50X Tris-Acetate for Electrophoresis (TAE)

## **50X Tris-Acetate for Electrophoresis (TAE)**

1. to 600 ml distilled H2O add:
	* 242 g Tris Base
	* 57.1 ml glacial Acetic Acid
	* 100 ml 0.5M EDTA (pH 8.0)
2. Mix and completely dissolve
3. Adjust final volume to 1L and remix
4. Label and pour into 1L bottle
5. Autoclave for sterilization

## **Making 2L of 50X TAE**

1. to 1200 ml distilled H2O add:
	* 484 g Tris Base
	* 114.2 ml glacial Acetic Acid
	* 200 ml 0.5M EDTA (pH 8.0)
2. Mix and completely dissolve
3. Adjust final volume to 1L and remix
4. Label and pour into 1L bottle
5. Autoclave for sterilization
PCR from Genomic Prep

* 1 ul genomic DNA
* 1.5 ul 10uM each primer
* 10 ul Phusion buffer
* 5 ul 2mM dNTPs
* dH2O to 49ul
* add 1 ul Phusion

**PCR Program:**

* 94**°** 30 sec
* 35 cycles:
	+ 94**°** 30 sec
	+ 5**°** below the melting temperature of your primers 30 sec
	+ 72**°** 1 min (the standard is 30 sec-1 min/ kb of PCR product)
* 72**°**10 min
* Hold at 15**°**

If that doesn't work, try a **Touchdown PCR**:

* 94**°** 30 sec
* 10 cycles
	+ 94**°** 30 sec
	+ 1**°** degree above the melting temperature of your primers 10 cycles and subtract 0.5**°** with every cycle (total of 5**°** ) 30 sec
	+ 72**°** 1 min (the standard is 30 sec – 1 min/ kb of PCR product)
* 25 cycles
	+ 98**°** 30 sec
	+ 4**°**degrees below the melting temperature of your primers 30 sec 25 cycles
	+ 72**°** 1 min (the standard is 30 sec – 1 min/ kb of PCR product)
* 72**°** 10 min
* Hold at 15
YWAW: 2MGD

**YWAW: 2MGD (YWAW: 2 Minigames Demo!) - последняя демоверсия игры YWAW!**
В этой демо будут две миниигры Ад и Рай.

**На данный момент нет ни одного скриншота!**

## **АД!**

**Вам нужно будет убежать от компьютерного вируса (имя его не известно), тем самым собирать монеты, которые понадобятся для прокачки игрока в компьютерном мире! Вам нужно будет добежать до финиша .**

## **РАЙ!**

'*Вам нужно будет прыгать по облакам, и собирать монеты, но всё так просто, постепенно зона будет сужаться! Если она настигнет вас это проигрыш! '***Вам нужно будет добежать до финиша.**
O Peixe

***O Peixe*** é um curta-metragem de animação brasileiro, produzido pela Lincoln Animation e lançado originalmente em 07 de Fevereiro de 2015. É o quinto curta em animação da Lincoln's e o segundo da série Cheshire Cat. O curta levou três anos para ser finalizado, sendo na época a produção de duração mais longa do estúdio a ser concluída com sucesso. Foi baseado em uma tirinha de Cascão, personagem da Turma da Mônica criado por Maurício de Sousa. Fez críticas aos movimentos políticos da época no Brasil contra o governo da Presidente Dilma Rousseff e a realização da Copa do Mundo de 2014 no país. Também fez referência ao período da crise hídrica do estado de São Paulo naquele ano. É o curta de estreia de Beethoven Fisher, personagem criado em homenagem a um peixinho beta que pertencia ao irmão de Lincoln, Victor.
WASHABLE

```
------------------------------------------------------ИНФОРМАЦИЯ------------------------------------------------

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> Внимание у каждого монитор разного размер и такая надпись может выглядеть странно
>
>

## WASHABLE

### Как получить?

DONTABLE SHOOTABLE ONABLE APPLEABLE

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-------------------------------------------------------------ГАЛЕРЕЯ------------------------------------------------

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Meleata

**Meleata** is a dish native to Sylh Shallow.

## General Information

Meleata is seasoned rice that’s been boiled with milk and dried beef. It’s common among soldiers because it’s easy to make and store. There are different recipes, as everyone makes it differently, affecting the taste with their own seasonings.

## Trivia

* Solt seasons his Meleata with oranges and cinnamon.
Lincoln Animation

***A Lincoln Animation*** é um estúdio de cinema amador fundado em 17 de Setembro de 2010 em Santo André, no estado de São Paulo, Brasil. Originalmente era um estúdio só, sendo divido em duas unidades em 2011. A reunificação das duas unidades de animação aconteceu durante os eventos chamados "Reforma Linck", iniciada em 2014. A Lincoln Animation foi o único estúdio da Lincoln's até 2015, quando Lincoln iniciou as produções live-actions do estúdio. Enquanto o estúdio de produções live assumiu o nome da companhia até aquele momento (A Lincoln Studios-Film), o estúdio de animação assumiu o Lincoln Animation. Os dois estúdios juntos formam o que chamamos de Lincoln's, que inclui também o canal do YouTube, as redes sociais, a Lincoln Records e o Theatro INA.
Cheshire Cat

***Cheshire Cat*** foi uma série cinematográfica produzida pela Lincoln's. Foi a primeira série oficial de animação da Lincoln Animation. A série mostra as peripécias de Cheshire, personagem principal da Lincoln's, e algumas interações entre ele e outros personagens dos The Crazies. Muitos personagens fizeram suas estreias nessa série. Teve um total de 3 curtas produzidos e muitos outros iniciados, porém algumas produções previstas para a série foram transferidas para "The Crazies", a série sucessora de "Cheshire Cat", e algumas outras foram canceladas durante os anos.
Хоумбрю правила

ФОРМАТИРУЕТСЯ

## **Правила, что были добавлены лично мастером.**

### *Очки отсылок:*

Обычно, на играх присутствует множество отсылок на различных персонажей из книг, комиксов, сериалов и тд, на ситуации, реплики и тому подобное. За их нахождение вы будете получать О*чки отсылок.* Собирать их можно неограниченное количество, а тратить на переброс мелких бросков не особо влияющих на игровой процесс или же конвертировать в *Очки судьбы* в соотношении 3 к 1.

### *Очки судьбы:*

Очки, что дарованы вам для изменения хода ситуации. Со старта их даётся 3 и больше этого числа быть не может. Заработать их можно путём разрешения сложных задач и ситуаций без помощи мастера, необычное исполнения различных идей и т.д. Тратятся на переброс кубиков, влияющих на успех того или иного действия:

```
- 1 очко судьбы - Переброс кубика.

```

```
- 2 очка судьбы - Бросок сразу считается успешным.

```

```
- 3 очка судьбы - Бросок считается критическим успехом.

```

По скольку их использование даёт возможность совершить невозможное, то разумеется, в некоторых ситуациях, использование очков судьбы запрещается, уточняйте у мастера.

## Система карт CardBox

CardBox - колода из 191 карты с эффектами, делящимися на 4 тира: бронзовые, серебряные, золотые, платиновые. Само собой, чем выше тир, тем более сильные эффекты предлагает карта.

Получить карту из желанной коробочки можно будет следующими путями:

- Обменять на неё 3 очка судьбы.

- Идеально пройти заготовленную ситуацию/модуль [По мнению ДМ]

- Принять в качестве благодарности за помощь ДМ'у [В том числе и материальную]

Полезная информация:

- Получение карты из CardBox'а происходит путём прокидывания 1d191 кубика.

- Карта привязывается непосредственно к игроку и может быть использована всего 1 раз на любом из его персонажей.

- Активация карты происходит путём её сброса с руки игрока. Действия и ходы персонажа на её использование не требуются.

- Если карта вам не понравилась, вы можете распылить её перед/после игры или когда персонаж совершает короткий или продолжительный отдых по следующему принципу:

* Платиновая карта = 3 очка судьбы

* Золотая карта = 2 очка судьбы

* Серебряная, бронзовая карта = 1 очко судьбы.

Ниже приведены примеры таких карт:
Noah

## Affiliations

### Nation

* United States of America (formerly)
* Emberfall (formerly)
* Sylh Shallow

### Residence

* Washington, D.C (formerly)
* Ironrose Castle, Emberfall (formerly)
* Crystal Palace, Syhl Shallow

### Occupation

* Doctor
* Healer

## Series Information

### Appearances

* *A Curse So Dark and Lonely*
* *A Heart So Fierce and Broken*
* *A Vow So Bold and Deadly*
* *Forging Silver into Stars*

**Noah** is a former doctor and Jake's boyfriend.
Lilith

art by montherox

**Lilith** was the magesmith that cursed Rhen to repeat the autumn of his eighteenth birthday until a girl fell in love with him.

## Early Life

Lilith arrived at King Broderick's palace one day for Prince Rhen's eighteenth birthday celebration. She decided to dance with King Broderick, then she afterward moved on to dance with Prince Rhen, and later on went on upstairs to head to Rhen's room. She was stopped by Grey guarding Rhen's door, along with other guards in the hallway. Lilith ended up bribing Grey into letting her into Rhen's room for the night.

Rhen ended up using Lilith as a "one night stand" and rejected her. Lilith was furious because of this rejection, and she embed the notorious curse on Rhen.

## Physical Description

Lilith was described as extremely beautiful. She featured soft skin, pink cheeks and rose-colored lips. She had hair the color of a raven's wing, with perfect curls falling over her shoulder. She often wore silk dresses that accentuated her curves, narrow waist, and the rise of her breast. After Grey tried to take her life, she was left with a terrible scar across her neck.
Lawrence

**Lawrence** is the man who Harper's father owed money to, and who her brother Jake is working for to keep him from going after the rest of the family. Lawernce forces Jake to hurt people and scare them into giving Lawerence his money.

## History

### A Curse So Dark And Lonely

Lawrence comes with some thugs and attacks Harper, Jake, Noah and Grey, shooting at them. They narrowly escape thanks to Grey teleporting all four off them to Emberfall.
Feeding RNAi protocol

**Day 1:**

* Start 20ml LB + carbenicillin cultures of your bacterial strain containing the RNAi vector.
* Don’t forget your empty vector control (L4440)!
* Grow overnight at 37º.
* Chunk worms and put at 20°

**Day 2:**

* In the morning, spin down the overnight cultures (5 min at 4,000 rpm in the table top centrifuge works), and resuspend them in 1ml total volume. This stock is enough for 10 plates and can be kept for up to a week at 4º.
* Put 100µl/plate of the resuspended bacteria onto NGM + IPTG + carbenicillin
* After the plates have dried, put plates at 37º to induce RNA expression overnight

**Day 3:**

* The next morning, put ~100µl of M9 onto an unspread NGM plate. You can also use the cover of one of your RNAi plates. Pick wildtype L4s into the M9 to rinse. You can pipette up and down if you want.
* Pick L4s from the M9 and put them on one of the plates seeded the day before with RNAi bacteria. You can also pipette them into the plate and let it dry, assuming that there is not very much OP50 in the M9. Put them at 20º and let them crawl around eating the bacteria for ~2 hrs. This should get rid of any residual OP50 in their gut.
* After 2 hrs, transfer the worms to fresh plates (seeded the day before with RNAi bacteria). Put 3-4 worms/plate and keep at 20º to let the parents self. Put the remaining plates at 4°.

**Day 6 or 7:**

* If your experiment requires staged worms (i.e. immunofluorescence or scoring apoptosis), pick L4s to the RNAi plate stored at 4° and put at 20º to perform the experiment the next day.
Solt

Captain **Solt** is a military member from Syhl Shallow.

## Biography

### A Vow So Bold and Deadly

Upon first meeting with Grey, the two men seem to be at odds. As part of Sylh Shallows military, solt is expected to follow his queen's orders, and while he did so under the reign of Karis Luran, he is slower to answer to her successor Lia Mara. He also defies Grey at every turn, even though he is to command the army into a war with Emberfall's Crown Prince Rhen. At one point, Grey orders Solt to fight numerous times, until he becomes sluggish and is easily defeated by younger recruits. Lia Mara ultimately stops this, telling solt that Grey would have had him fight until he couldn't hold his sword anymore. After Lia Mara leaves, Grey confides that she was wrong and he would have tied Solt's sword to his hands and kept him fighting.

## Personality

Solt is a soldier and behaves as one. he is fast to follow orders, exactly how they were told to him. He seemingly craves a certain degree of violence,. for someone to gain his respect, seen in his different behaviour under Queen Karis Luran and later her daughter Lia Mara, whom he doubts every so often.
Lia Mara

Official art by montherox

> Ah yes, the most dangerous person at the party is always the girl sitting alone with a book.
>
> Lia Mara, *A Heart So Fierce and Broken*

**Lia Mara** is the current queen of Sylh Shallow.

## Biography

### Early life

Lia Mara was born to Karis Luran, the late queen of Sylh Shallow and an unnamed general. She has one younger sister Nolla Verin; they were both raised by their mother in the Crystal Palace.

Despite being the eldest daughter, her mother decided that she would not be the one to inherit the throne, for she had a sweet character, and therefore her mother did not believe her capable of being queen. As result, her younger sister, Nolla Verin was named as heir to the throne.

### A Heart So Fierce and Broken

After a long carriage ride with her mother, sister, and escorts, they arrive at Ironrose Castle to have an audience with Rhen and form an alliance in which Karis Luran offers Nolla Verin as a wife. The audience does not go well, and they are forced to leave the castle.

On the way back to her land, Lia Mara takes her escorts and returns to Ironrose Castle without saying anything to her mother and sister. Her idea was to respectfully negotiate with Rhen, but he outsmarts her and kidnaps her, killing her guard (who she is friends with) in the process.

Locked in a room in the castle, Lia Mara regrets trusting Rhen, and thinking of a way to run away, she discovers a way to move the back wall of the fireplace and thus enter the other room, and doing so, discovers that the occupant of the room next door is Grey, who had also been taken hostage by Rhen. She hides behind the curtains but Grey discovers her and at first, doesn't trust her, but he doesn't give her away.

When Rhen takes Tycho and Grey to whip them and Grey knocks everyone unconscious. Lia Mara helps them escape and tells him she will give them asylum in Syhl Shallow and intercede for him with the queen as she discovers that he is the true heir if they guarantee her a safe return to her land.

## Personality

Lia Mara's personality traits differ greatly from the rest of her family. She abhors violence and instead values gentleness and compassion. She is often unsure about herself, questioning her own strength. She is very intelligent and clever; people often underestimate how clever she really is.

## Physical Description

Lia Mara has green eyes and red hair that she usually wears in a braid. She is tall and slender, with a fair complexion and a graceful posture. She likes to wear simple dresses in soft colors, such as blue, green, or purple, with lace or embroidery details. She also wears a silver necklace with a pendant that resembles a heart, which was given to her by Grey, the commander of the royal guard and her love interest.
Криг

## Бог Ремесел

Был скован и высечен с первым первым орудием ремесла, когда всем была нужна помощь в этом не лёгком деле. Криг помогает ценителям своего труда, вкладывающим всю душу в ремесленное дело неожиданным вдохновением и стремлением к новым вершинам. Его заповедью стало: "Дело мастера боится", а символом искры, летящие от наковальни.
Jax

by whimsicalillustration

> A bit of kindness chased by a bit of cruelty.
> Typical.
>
> Jax, *Forging Silver into Stars*

**Jax** is a blacksmith.

## Biography

Jax is best friends with Callyn, they are struggling to survive in the small town of Briarlock. Once Tycho's horse, Mercy, loses a shoe when he was carrying an important message between Ironrose Castle and the Crystal Palace, so he goes looking for a blacksmith and met Jax.

Like his best friend Callyn, Jax is 18 years old, and he's been working with his father at their blacksmith forge since he was old enough to wield a hammer.

Jax's life has been full of misfortune, from the fact that his mother died when he was born, to the accident that crushed his foot when he was thirteen, to the knowledge that his father spent all their tax money on liquor and gambling, and now their home is in danger.

When a mysterious noblewoman arrives with a handful of silver in exchange for holding a sealed message, Jax knows he has no choice, even though he suspects he's being asked to carry word of a threat against the throne. Far from the palace in their little border town of Briarlock, both Jax and Callyn think there's little risk in holding a dangerous message, until Lord Tycho, the King's Courier, arrives in town.

## Physical Description

Jax has dark hair and light hazel eyes. He is missing his right foot due to an accident when he was thirteen years old. He was helping his father fix a wagon axle and it collapsed on his leg and crushed his foot.

## Trivia

- Jax is currently in a romantic relationship with the King's Courier, Lord Tycho
Raina

**Raina** is a young girl, living in Blind Hollow.

## A Heart So Fierce And Broken

Together with her sister and her father, a trapper, Raina was scouring the woods for animals. There, the family stumbled upon the camp of Queen Karis Luran and her daughters. While her father and her sister were caught, Raina was able to hide, effectively seeing them being killed by the queen's guards, after Nolla Verin gave the command to do so. Staying in hiding, Raina was found by Lia Mara and her own guard Parrish. Both of them saw her, but let her go.

Later, back in her village, Raina was called by the barkeep Eowen to show three strangers the way to the village's blacksmith. Raina discovers one of them being Lia Mara and alarms Eowen. After the group around Lia Mara flees, Raina and the villagers find them engaged with the Royal Guard and help them in a fight, revealing that Raina remembers Lia Mara as the one who saved her.
Sinna Cataleha

## Biographical Information

### Age

Unborn (during A Vow So Bold and Deadly)3 (during Forging Silver into Stars)

## Affiliations

### Nation

* Emberfall
* Syhl Shallow

## Relationships

### Family

* Grey (father)
* Lia Mara (mother)
* Unborn Sibling † (unnamed)
* Nolla Verin (aunt)
* Rhen (uncle)
* Arabella † (aunt)
* Isadore † (aunt)
* King Broderick † (paternal grandfather)
* Paternal Grandmother † (unnamed)
* Karis Luran † (maternal grandmother)
* Maternal Grandfather (unnamed)

## Series Information

### Mentions

*A Vow So Bold and Deadly*

**Sinna Cataleha**, is the daughter of Grey and Lia Mara.
Karis Luran

**Karis Luran** was the late queen of Sylh Shallow.

## Biography

### A Curse So Dark and Lonely

Karis Luran has had a long time animosity with the royal family of Emberfall. For a long time she has planned to take Emberfall with her powerful army of soldiers.

When Rhen transforms into the monster, she uses that opportunity to attack with her army.

## Physical Description

Karis Luran had creamy skin, bloodred hair that hangs straight to her shoulders, and dark gold eyes. She was described as someone who is not beautiful, but is striking, with no kindness in her eyes. She had a presence that commanded the attention of everyone in the room immediately.

## Relationships

### Lia Mara

Lia Mara is Karis Luran's first daughter, but isn't named heir, because Karis Luran considered her too kind. Instead she named her youngest daughter Nolla Verin as her heir.

### Nolla Verin

Nolla Verin is Karis Luran's youngest daughter and chosen heir to the throne of Sylh Shallow. She also wished for the best for Nolla and encourages her to do the "right thing".
Brigid Kemmerer

**Brigid Kemmerer** is the author of the Cursebreakers trilogy and the Forging Silver into Stars trilogy.

## Personal life

Brigid Kemmerer was born on January 11, 1978, in Omaha, Nebraska, United States. As a child, her parents moved throughout the United States, from Albuquerque, New Mexico, to Cleveland, Ohio, until they settled near Annapolis, Maryland. She worked in the finance industry before becoming a full-time writer.

She currently lives in Baltimore sea with her husband, and sons.

## Bibliography

### Series

#### Elemental

* *Elemental*
* *Storm*
* *Fearless*
* *Spark*
* *Breathless*
* *Spirit*
* *Secret*
* *Sacrifice*

#### Letters to the Lost

* *Letters to the Lost*
* *More than We Can Tell*

#### Cursebreakers trilogy

* *A Curse So Dark and Lonely*
* *A Heart So Fierce and Broken*
* *A Vow So Bold and Deadly*

#### Forging Silver into Stars trilogy

* *Forging Silver into Stars*
* *Untitled 2nd book*
* *Untitled 3rd book*

#### Defy the Night

* *Defy the Night*
* *Defend the Dawn*
* *Destroy the Day*

### Standalones

* *Thicker Than Water*
* *Call It What You Want*

## External links

* Brigid Kemmerer on Wikipedia.
* Brigid Kemmerer's official site
* Brigid Kemmerer (@BrigidKemmerer) on Twitter.
* Brigid Kemmerer (@brigidkemmerer) on Instagram.
* Brigid Kemmerer on Facebook.
* Brigid Kemmerer on Tumblr.
* Brigid Kemmerer on Youtube.
* Brigid Kemmerer on Goodreads.
* Brigid Kemmerer on Pinterest.

## References

1. ↑ Brigid's Bloomsbury profile
2. ↑ Capital Gazette article
Cursebreakers Wiki

WELCOME TO THE CURSEBREAKERS WIKI

The Cursebreakers Wiki is dedicated to Brigid Kemmerer's book series, Cursebreakers trilogy and the spin-off Forging Silver into Stars trilogy!.

***Warning!*** *Please be advised that **this wiki contains major spoilers!** Read at your own risk!*

Wiki rules • Categories • Pages to Add • Pages that need more Info

BOOK SERIES

CURSEBREAKERS

FORGING SILVER INTO STARS

CHARACTERS

*Harper**Rhen**Grey**Lia Mara*

AFFILIATES

Defy the Night WikiSorcery of Thorns Wiki
Iisak

art by montherox

**Iisak** is a Scraver, who was held captive at Worwick's Tourney, but later freed by Grey.

## Physical Description

Iisak is described as somewhat human-shaped, but featuring dark gray skin. He has wings sprouting from his back - which measure a span of 12 feet -, and a curly tail. Furthermore, he has clawed hands and feet, and a shock of black hair.
Sorra

**Sorra** was a person guard to Lia Mara.

## Biography

### A Heart so Fierce and Broken

She is one of Lia Mara's personal guards. When Lia Mara attempts to meet with Crown Prince Rhen to discuss peace, she travels with her. When Rhen makes his intention to use Lia Mara as leverage clear, Sorra attacks the guards that grab Lia Mara stabbing 3 with a dagger before grabbing a sword and killing a 4th, she is then stabbed by another guard killing her almost instantly.

## Physical Description

Sorra had dark brown hair, which she usually had bound into a tight braid that hung trapped beneath her armor. She was described as having a beautiful face and a lean built. It is mentioned she wore no makeup.
Forging Silver into Stars trilogy

**Forging Silver into Stars‎** is a series of fantasy novels by young adult literature author Brigid Kemmerer. It consists of Forging Silver into Stars, an untitled second, and third book.

The series centers around Tycho 4 years after the events of A Vow So Bold and Deadly.

## Trivia

* The book covers are designed by Shane Rebenschied.
Callyn

by whimsicalillustration

> I used to dream of magic.
>
> Callyn, *Forging Silver into Stars*

**Callyn** is a baker.

## Biography

Callyn is eighteen years old, and she's been left to raise her younger sister, Nora, and run her family's bakery after her parents died. She lives far from any royal circles, and she just wants to bake bread and look after her sister -- and she might have a bit of a crush on her best friend, Jax, the blacksmith who lives down the lane. Callyn isn't afraid of much, but she hates magic: her mother was killed by a cursed beast during the war against Emberfall, and her father was killed during an assault on the Crystal Palace, when the people tried to take a stand against their magic-wielding new king. Callyn wants nothing to do with royalty or magic, but her world is turned upside down when she and Jax find themselves involved in a plot against the throne.
Emberfall

**Emberfall** is a kingdom ruled by Grey. It was formerly ruled by the Crown Prince Rhen.

## General Information

The royal family of Emberfall resides in Ironrose Castle, right at the heart of Emberfall.

## History

During Rhen's grandfather's rule, a small colony of magesmiths from the western colony of Verin took refuge in Emberfall near the northern border. They were the last remaining magesmiths and the King of Verin had sought to destroy them all, so they fled east. They swore alliance to Rhen's grandfather and caused few problems. They would sell their spells to the people, and the King saw it as an indulgence to allow it. Their magic was small and harmless. The King would tax them heavily for the privilege of practicing it, resulting in tensions that the King either ignored or simply did not care about. When the King's son reached marrying age, a young woman visited the castle, presenting herself as a candidate for marriage. But she was a magesmith in disguise, and once she was there, she bewitched the King's son to try to trick him into marriage.

She was not very powerful and the guards were able to imprison and later execute her, once she confessed. But the King took out his wrath on the magesmiths who remained and banned magic from the kingdom. He sent an army because it was said that as each one of the magesmiths was killed, the magic would be passed on to the others, making the magesmiths who remained, increasingly powerful. To avoid that, they had to slaughter them all at once, and so they did.

One escaped, or was hidden, and she appeared on the night of the Crown Prince Rhen's eighteenth birthday, dressed as a courtier, ready to seduce the prince. She had the strongest magic because she had absorbed all the magic of the rest of her kind who were dead.

She was not solely seeking revenge for her people but rather wanted to align herself with the royal family. Rhen turned her away after spending a night with her, allowing her to believe their night meant more than it did.

She cursed the Prince and the territory of Ironrose castle to repeat the same season, over and over, until he finds true love. The prince also becomes a monster who terrorizes and kills the people and plunges the country into poverty and terror.

After the Prince is able to remove the curse, it is discovered he had an older brother, Grey, who was the rightful heir to the throne. In the end, Grey ends up becoming the King and rules over all of Emberfall while Rhen is stripped of his status and becomes his advisor.

## Royal Insignia

The royal insignia of the Kingdom of Emberfall is a lion and a rose embossed in gold.
Syhl Shallow

**Syhl Shallow** is a country far northwest of Emberfall.

## General Information

Sylh Shallow is a neighbouring country of Emberfall, located on the other side of the shared mountain range. During the time of King Broderick's reign, the two monarchs had no quarrel with each other. The country is landlocked, with brutal winters, and dangerous game animals.

Syhl Shallow was previously ruled by the cruel queen Karis Luran, until her daughter Lia Mara killed her.
Tycho

* Cursebreakers
* Forging Silver into Stars

**Tycho** is a young stable boy, formerly sworn to Worwick.

## Biography

Tycho was a 15 year old boy who was working at Worwick's tourney with Grey.

### Forging Silver into Stars

Tycho is now 19 years old, working as the King's Courier, charged with delivering confidential messages between Syhl Shallow and Emberfall. Tycho is well aware of the problems magic can cause, but he also knows the benefits, and he's been gifted with rings made from Iishellasan steel that grant him certain abilities.

When his horse, Mercy, throws a shoe, Tycho ends up in Briarlock, seeking a blacksmith, where he meets Callyn and Jax and finds a lot more trouble than he bargained for.

## Physical Description

Tycho is described as being small for his age, with a wiry build that makes him look closer to twelve than fifteen. He has dark-blond hair that hangs just past his chin, and blue eyes.

## Trivia

* Tycho is afraid of soldiers, with Noah mentioning that he might be suffering from PTSD.
* Tycho is bisexual.

## References

1. ↑ Brigid Kemmerer on Instagram stories