Resonance energy transfer

File:FRET Jablonski diagram.svg
**Förster resonance energy transfer** (**FRET**), **fluorescence resonance energy transfer** (**FRET**), **resonance energy transfer** (**RET**) or **electronic energy transfer** (**EET**) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores). A donor chromophore, initially in its electronic excited state, may transfer energy to an acceptor chromophore through nonradiative dipole–dipole coupling. The efficiency of this energy transfer is inversely proportional to the sixth power of the distance between donor and acceptor, making FRET extremely sensitive to small changes in distance.

Measurements of FRET efficiency can be used to determine if two fluorophores are within a certain distance of each other. Such measurements are used as a research tool in fields including biology and chemistry.

FRET is analogous to near-field communication, in that the radius of interaction is much smaller than the wavelength of light emitted. In the near-field region, the excited chromophore emits a virtual photon that is instantly absorbed by a receiving chromophore. These virtual photons are undetectable, since their existence violates the conservation of energy and momentum, and hence FRET is known as a *radiationless* mechanism. Quantum electrodynamical calculations have been used to determine that radiationless (FRET) and radiative energy transfer are the short- and long-range asymptotes of a single unified mechanism.

## Terminology

 File:Concept of FRET.png
Förster resonance energy transfer is named after the German scientist Theodor Förster. When both chromophores are fluorescent, the term "fluorescence resonance energy transfer" is often used instead, although the energy is not actually transferred by fluorescence. In order to avoid an erroneous interpretation of the phenomenon that is always a nonradiative transfer of energy (even when occurring between two fluorescent chromophores), the name "Förster resonance energy transfer" is preferred to "fluorescence resonance energy transfer"; however, the latter enjoys common usage in scientific literature. FRET is not restricted to fluorescence and occurs in connection with phosphorescence as well.

## Theoretical basis

The FRET efficiency (**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle E}**
) is the quantum yield of the energy-transfer transition, i.e. the probability of energy-transfer event occurring per donor excitation event:

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle E = \frac{k\_\text{ET}}{k\_f + k\_\text{ET} + \sum{k\_i}},}**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle k\_\text{ET}}**
 is the rate of energy transfer, **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle k\_f}**
 the radiative decay rate of the donor, and **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle k\_i}**
 the rates of any other de-excitation pathways excluding energy transfers to other acceptors.

The FRET efficiency depends on many physical parameters that can be grouped as: 1) the distance between the donor and the acceptor (typically in the range of 1–10 nm), 2) the spectral overlap of the donor emission spectrum and the acceptor absorption spectrum, and 3) the relative orientation of the donor emission dipole moment and the acceptor absorption dipole moment.

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle E}**
 depends on the donor-to-acceptor separation distance **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle r}**
 with an inverse 6th-power law due to the dipole–dipole coupling mechanism:

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle E = \frac{1}{1 + (r/R\_0)^6}}**

with **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle R\_0}**
 being the Förster distance of this pair of donor and acceptor, i.e. the distance at which the energy transfer efficiency is 50%.
The Förster distance depends on the overlap integral of the donor emission spectrum with the acceptor absorption spectrum and their mutual molecular orientation as expressed by the following equation:

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle {R\_0}^6 = \frac{2.07}{128 \, \pi^5 \, N\_A} \, \frac{\kappa^2 \,Q\_D}{n^4} {\int F\_{\rm D}(\lambda) \, \epsilon\_{\rm A}(\lambda) \, \lambda^4 \, d\lambda} }**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle Q\_\text{D}}**
 is the fluorescence quantum yield of the donor in the absence of the acceptor, **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \kappa^2}**
 is the dipole orientation factor, **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle n}**
 is the refractive index of the medium, **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle N\_\text{A}}**
 is the Avogadro constant, and **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle J}**
 is the spectral overlap integral calculated as

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle J = \frac{\int f\_\text{D}(\lambda) \epsilon\_\text{A}(\lambda) \lambda^4 \, d\lambda}{\int f\_\text{D}(\lambda) \, d\lambda} = \int \overline{f\_\text{D}}(\lambda) \epsilon\_\text{A}(\lambda) \lambda^4 \, d\lambda,}**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle f\_\text{D}}**
 is the donor emission spectrum, **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \overline{f\_\text{D}}}**
 is the donor emission spectrum normalized to an area of 1, and **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \epsilon\_\text{A}}**
 is the acceptor molar extinction coefficient, normally obtained from an absorption spectrum.
The orientation factor κ is given by

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \kappa = \hat\mu\_\text{A} \cdot \hat\mu\_\text{D} - 3 (\hat\mu\_\text{D} \cdot \hat R) (\hat\mu\_\text{A} \cdot \hat R), }**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \hat\mu\_i}**
 denotes the normalized transition dipole moment of the respective fluorophore, and **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \hat R}**
 denotes the normalized inter-fluorophore displacement.
**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \kappa^2}**
 = 2/3 is often assumed. This value is obtained when both dyes are freely rotating and can be considered to be isotropically oriented during the excited-state lifetime. If either dye is fixed or not free to rotate, then **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \kappa^2}**
 = 2/3 will not be a valid assumption. In most cases, however, even modest reorientation of the dyes results in enough orientational averaging that **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \kappa^2}**
 = 2/3 does not result in a large error in the estimated energy-transfer distance due to the sixth-power dependence of **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle R\_0}**
 on **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \kappa^2}**
. Even when **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \kappa^2}**
 is quite different from 2/3, the error can be associated with a shift in **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle R\_0}**
, and thus determinations of changes in relative distance for a particular system are still valid. Fluorescent proteins do not reorient on a timescale that is faster than their fluorescence lifetime. In this case 0 ≤ **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \kappa^2}**
 ≤ 4.

For time-dependent analyses of FRET, the rate of energy transfer (**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle k\_\text{ET}}**
) can be used directly instead:

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle k\_\text{ET} = (\frac{R\_0}{r})^6 \, \frac{1}{\tau\_D}}**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \tau\_D}**
 is the donor's fluorescence lifetime in the absence of the acceptor.

The FRET efficiency relates to the quantum yield and the fluorescence lifetime of the donor molecule as follows:

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle E = 1 - \tau'\_\text{D}/\tau\_\text{D},}**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \tau\_\text{D}'}**
 and **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \tau\_\text{D}}**
 are the donor fluorescence lifetimes in the presence and absence of an acceptor respectively, or as

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle E = 1 - F\_\text{D}'/F\_\text{D},}**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle F\_\text{D}'}**
 and **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle F\_\text{D}}**
 are the donor fluorescence intensities with and without an acceptor respectively.

## Experimental confirmation of the Förster resonance energy transfer theory

The inverse sixth-power distance dependence of Förster resonance energy transfer was experimentally confirmed by Wilchek, Edelhoch and Brand using tryptophyl peptides. Stryer, Haugland and Yguerabide also experimentally demonstrated the theoretical dependence of Förster resonance energy transfer on the overlap integral by using a fused indolosteroid as a donor and a ketone as an acceptor. However, a lot of contradictions of special experiments with the theory was observed. The reason is that the theory has approximate character and gives overestimated distances of 50–100 ångströms.

## Methods to measure FRET efficiency

In fluorescence microscopy, fluorescence confocal laser scanning microscopy, as well as in molecular biology, FRET is a useful tool to quantify molecular dynamics in biophysics and biochemistry, such as protein-protein interactions, protein–DNA interactions, and protein conformational changes. For monitoring the complex formation between two molecules, one of them is labeled with a donor and the other with an acceptor. The FRET efficiency is measured and used to identify interactions between the labeled complexes. There are several ways of measuring the FRET efficiency by monitoring changes in the fluorescence emitted by the donor or the acceptor.

### Sensitized emission

One method of measuring FRET efficiency is to measure the variation in acceptor emission intensity. When the donor and acceptor are in proximity (1–10 nm) due to the interaction of the two molecules, the acceptor emission will increase because of the intermolecular FRET from the donor to the acceptor. For monitoring protein conformational changes, the target protein is labeled with a donor and an acceptor at two loci. When a twist or bend of the protein brings the change in the distance or relative orientation of the donor and acceptor, FRET change is observed. If a molecular interaction or a protein conformational change is dependent on ligand binding, this FRET technique is applicable to fluorescent indicators for the ligand detection.

### Photobleaching FRET

FRET efficiencies can also be inferred from the photobleaching rates of the donor in the presence and absence of an acceptor. This method can be performed on most fluorescence microscopes; one simply shines the excitation light (of a frequency that will excite the donor but not the acceptor significantly) on specimens with and without the acceptor fluorophore and monitors the donor fluorescence (typically separated from acceptor fluorescence using a bandpass filter) over time. The timescale is that of photobleaching, which is seconds to minutes, with fluorescence in each curve being given by

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \text{background} + \text{constant} \cdot e^{-\text{time}/\tau\_\text{pb}},}**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \tau\_\text{pb}}**
 is the photobleaching decay time constant and depends on whether the acceptor is present or not. Since photobleaching consists in the permanent inactivation of excited fluorophores, resonance energy transfer from an excited donor to an acceptor fluorophore prevents the photobleaching of that donor fluorophore, and thus high FRET efficiency leads to a longer photobleaching decay time constant:

**Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle E = 1 - \tau\_\text{pb}/\tau\_\text{pb}',}**

where **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \tau\_\text{pb}'}**
 and **Failed to parse (SVG with PNG fallback (MathML can be enabled via browser plugin): Invalid response ("Math extension cannot connect to Restbase.") from server "https://wikimedia.org/api/rest\_v1/":): {\displaystyle \tau\_\text{pb}}**
 are the photobleaching decay time constants of the donor in the presence and in the absence of the acceptor respectively. (Notice that the fraction is the reciprocal of that used for lifetime measurements).

This technique was introduced by Jovin in 1989. Its use of an entire curve of points to extract the time constants can give it accuracy advantages over the other methods. Also, the fact that time measurements are over seconds rather than nanoseconds makes it easier than fluorescence lifetime measurements, and because photobleaching decay rates do not generally depend on donor concentration (unless acceptor saturation is an issue), the careful control of concentrations needed for intensity measurements is not needed. It is, however, important to keep the illumination the same for the with- and without-acceptor measurements, as photobleaching increases markedly with more intense incident light.

### Lifetime measurements

FRET efficiency can also be determined from the change in the fluorescence lifetime of the donor. The lifetime of the donor will decrease in the presence of the acceptor. Lifetime measurements of the FRET-donor are used in fluorescence-lifetime imaging microscopy (FLIM).

## Fluorophores used for FRET

 File:Proteolytic cleavage of a Dual-GFP fusion FRET-pair.png
### CFP-YFP pairs

One common pair fluorophores for biological use is a cyan fluorescent protein (CFP) – yellow fluorescent protein (YFP) pair. Both are color variants of green fluorescent protein (GFP). Labeling with organic fluorescent dyes requires purification, chemical modification, and intracellular injection of a host protein. GFP variants can be attached to a host protein by genetic engineering which can be more convenient. Additionally, a fusion of CFP and YFP ("tandem-dimer") linked by a protease cleavage sequence can be used as a cleavage assay.

### BRET

A limitation of FRET performed with fluorophore donors is the requirement for external illumination to initiate the fluorescence transfer, which can lead to background noise in the results from direct excitation of the acceptor or to photobleaching. To avoid this drawback, bioluminescence resonance energy transfer (or **BRET**) has been developed. This technique uses a bioluminescent luciferase (typically the luciferase from *Renilla reniformis*) rather than CFP to produce an initial photon emission compatible with YFP.

BRET has also been implemented using a different luciferase enzyme, engineered from the deep-sea shrimp *Oplophorus gracilirostris*. This luciferase is smaller (19 kD) and brighter than the more commonly used luciferase from *Renilla reniformis*. Promega has developed this luciferase variant under the product name NanoLuc.

### Homo-FRET

In general, "FRET" refers to situations where the donor and acceptor proteins (or "fluorophores") are of two different types. In many biological situations, however, researchers might need to examine the interactions between two, or more, proteins of the same type—or indeed the same protein with itself, for example if the protein folds or forms part of a polymer chain of proteins or for other questions of quantification in biological cells.

Obviously, spectral differences will not be the tool used to detect and measure FRET, as both the acceptor and donor protein emit light with the same wavelengths. Yet researchers can detect differences in the polarisation between the light which excites the fluorophores and the light which is emitted, in a technique called FRET anisotropy imaging; the level of quantified anisotropy (difference in polarisation between the excitation and emission beams) then becomes an indicative guide to how many FRET events have happened.

### Others

Various compounds beside fluorescent proteins.

## Applications

The applications of fluorescence resonance energy transfer (FRET) have expanded tremendously in the last 25 years, and the technique has become a staple in many biological and biophysical fields. FRET can be used as a spectroscopic ruler to measure distance and detect molecular interactions in a number of systems and has applications in biology and biochemistry.

### Proteins

FRET is often used to detect and track interactions between proteins. Additionally, FRET can be used to measure distances between domains in a single protein by tagging different regions of the protein with fluorophores and measuring emission to determine distance. This provides information about protein conformation, including secondary structures and protein folding. This extends to tracking functional changes in protein structure, such as conformational changes associated with myosin activity. Applied in vivo, FRET has been used to detect the location and interactions of cellular structures including integrins and membrane proteins.

### Membranes

FRET can be used to observe membrane fluidity, movement and dispersal of membrane proteins, membrane lipid-protein and protein-protein interactions, and successful mixing of different membranes. FRET is also used to study formation and properties of membrane domains and lipid rafts in cell membranes and to determine surface density in membranes.

### Chemosensory

 File:FRET probe for the detection of Cd2+.gif
FRET-based probes can detect the presence of various molecules: the probe's structure is affected by small molecule binding or activity, which can turn the FRET system on or off. This is often used to detect anions, cations, small uncharged molecules, and some larger biomacromolecules as well. Similarly, FRET systems have been designed to detect changes in the cellular environment due to such factors as pH, hypoxia, or mitochondrial membrane potential.

### Signaling pathways

Another use for FRET is in the study of metabolic or signaling pathways. For example, FRET and BRET have been used in various experiments to characterize G-protein coupled receptor activation and consequent signaling mechanisms. Other examples include the use of FRET to analyze such diverse processes as bacterial chemotaxis and caspase activity in apoptosis.

### Other applications

In addition to common uses previously mentioned, FRET and BRET are also effective in the study of biochemical reaction kinetics. FRET is increasingly used for monitoring pH dependent assembly and disassembly and is valuable in the analysis of nucleic acids. This technique can be used to determine factors affecting various types of nanoparticle formation as well as the mechanisms and effects of nanomedicines.

## Other methods

A different, but related, mechanism is Dexter electron transfer.

An alternative method to detecting protein–protein proximity is the bimolecular fluorescence complementation (BiFC), where two parts of a fluorescent protein are each fused to other proteins. When these two parts meet, they form a fluorophore on a timescale of minutes or hours.

## See also

* Dexter electron transfer
* Förster coupling
* Surface energy transfer
* Time-resolved fluorescence energy transfer

## External links

* Forster Resonance Energy Transfer Demonstration on YouTube
* FRET Imaging (Tutorial of Becker & Hickl, website)