Twiv 815 95

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95:01 with getting a vaccination
95:03 um
95:05 and the deal is that the shingrix
95:07 vaccination is a protein subunit vaccine
95:10 so there's no virus involved
95:12 so an antiviral is not gonna and
95:14 antiviral is not gonna deal with that
95:16 uh did you guys have a strong reaction
95:18 to shingrix
95:21 no little little stiffness in the arm
95:23 not bad no okay
95:25 uh kathy you're next yes christos writes
95:29 hello i love your show keep
95:31 communicating science you are very
95:32 unique in what you're doing i want to
95:34 hear your opinion on the use of tcid 50
95:37 to determine virus titers either with in
95:39 vitro assays or in patients i keep
95:42 seeing it on sars2 papers and to be
95:44 honest i hate it i feel like
95:46 quantification of infectious particles
95:48 should be measured only in plaque
95:49 forming units tcid50 mixes up cpe
95:53 general pathogenic effects synthesia and
95:55 so forth it doesn't seem right for
95:57 viruses like sars2 am i wrong here's a
96:00 neat little paper describing two mink
96:02 outbreaks
96:03 cheers christos
96:05 okay so i will tell you my take on
96:07 plaque assays versus tcid50s
96:11 they're both using cytopathic effect
96:16 and in some cases maybe cincitia or
96:18 whatever to measure
96:19 um
96:21 a platforming unit or um some kind of
96:24 pathogenesis that allows you to score a
96:27 particular well
96:29 uh in a tcid 50 assay
96:33 they're both quantitative
96:37 and if you just
96:38 state your units consistently in tcid50
96:42 units or platforming units i think it's
96:44 fine
96:45 uh one of my colleagues here
96:48 doesn't like plaque essays and says that
96:50 tcid50 tcid50s
96:52 are
96:53 better uh it may depend on your virus
96:56 what you're used to and so forth
96:58 but really
97:00 in my mind they're measuring the same
97:01 things but now let's hear what vincent
97:04 and rich think well i i don't do tcic
97:08 d50s but not all viruses will plac so
97:11 sometimes you have to do a tcid50 if you
97:13 want infectivity and i noticed that
97:15 companies tend to use tcid50s a lot
97:18 because i think you can do more high
97:20 throughput
97:21 with tci 50 than black assay is hard to
97:23 do in a high throughput but i agree with
97:25 they're both measuring cpe um and you
97:28 could even do both and make an
97:30 equivalence of the two if you wanted for
97:32 your particular virus i agree we we had
97:36 i
97:38 have done only plaque assays except for
97:41 i had a
97:43 a student once we were studying
97:46 a pox virus of seals
97:49 and actually
97:50 isolated this thing and it wouldn't
97:52 plaque
97:53 but uh it would cause a cytopathic
97:55 effect
97:57 i found it uh to me it's always been
97:59 cumbersome because i'm familiar with the
98:01 tcic 50 as you've got to visually
98:03 inspect all these wells and decide where
98:05 the cytopathic effect is but i think
98:07 there are sort of staining methods that
98:09 you can use
98:10 where you can use a plate reader and in
98:12 that case it would be a piece of cake
98:15 and i think some people use tcid50
98:17 because it's easier yeah
98:19 if you can do that kind of thing so but
98:21 i agree i think they're both measuring
98:24 virus replication
98:26 so
98:27 that's the key
98:28 and you know as cathy said some people
98:30 like one some people like the other it's
98:32 fine there's something satisfying about
98:33 a plaque because you can sort of relate
98:36 to the notion that a virus did this a
98:39 single virus set this off but that
98:42 doesn't really
98:44 tell you
98:45 uh everything you need to know about the
98:47 population of viruses that you stuck on
98:48 the plate okay
98:50 um
98:51 because plaque assays you know are
98:53 themselves not uh perfect in quantifying
98:56 all the potentially infectious particles
98:59 in the in the solution so from that
99:01 point of view
99:03 they both are a kind of a relative
99:05 measure of what's going on but they're
99:07 both measuring infectivity so cool
99:10 this paper is very nice that that uh
99:14 it was sent to us here
99:16 uh by christos about me galbraith
99:18 stars cov2 outbreaks on mink farms in
99:20 germany um greece
99:23 and it's very detailed it's cool tracks
99:26 it really well by the way they killed
99:28 all the minks in the farm banks in
99:30 denmark and netherlands after the
99:32 outbreak
99:33 i also learned that on
99:36 our latest twiv
99:39 last one is from bev
99:42 you'll probably have lots of people
99:44 telling you this but just in case
99:46 dogs eyes refresh at a faster rate than
99:49 ours
99:50 do
99:51 so what we see as a moving screen
99:54 is a flickering mess
99:58 here's the first link i found on it
100:01 so we were
100:03 thinking about whether dogs see tv
100:06 and uh this article in the nest is do
100:10 dogs see the tv the same as humans
100:14 dogs see a whole new picture
100:16 when it comes to tv because of the
100:18 flicker rate yeah
100:20 so what do they end up seeing they don't
100:23 have the same visual acuity
100:26 um with the advent of high resolution
100:28 broadcasting
100:30 hopefully television producers have
100:31 tapped into the canine market
100:36 dog tv a channel devoted to programming
100:39 for dogs launched in 2012.
100:41 it broadcasts a mix of relaxing and
100:44 stimulating content designed to reduce
100:46 stress boredom and loneliness while dogs
100:48 are alone in the house
100:51 i my neighbor who has like a dozen
100:53 schnauzers
100:55 they they rescue them
100:57 their tv when i go to my car i can see
101:00 the tv and it's on 24 7 to entertain the
101:05 dogs
101:06 you know you could go out any time of
101:08 day and it's on
101:10 so they see a flickering mess
101:13 i see
101:14 but clearly some dogs are mesmerized by
101:16 tv
101:17 yep well flickering mess could probably
101:19 be pretty entertaining could be
101:22 all right it's time for some picks
101:24 and i am really
101:26 looking forward to hearing the next
101:28 installment of dixon's top 10 comedians
101:31 well
101:32 this is number four as i recall
101:34 and uh he should have been number one
101:36 but i didn't alphabetize them or
101:38 anything i just randomly picked them mel
101:40 brooks is
101:42 by far
101:43 the most versatile of all the comedians
101:46 that i've
101:47 become attached to and my
101:49 that it tickles my funny bone every time
101:52 this man says something it's usually
101:54 funny
101:55 uh and he intends it to be and he's
101:57 quick clever
101:59 a remarkable individual
102:01 he's made movies he's done stand up
102:04 he's uh
102:06 selected incredible casts for some of
102:09 his movies
102:10 and this is a compilation of his life
102:12 basically it's a pretty long
102:14 youtube documentary on mel brooks and
102:18 he starts out by dancing with his wife
102:21 and singing um
102:24 sweet georgia brown in polish
102:27 and he and the documentary ends with the
102:30 same
102:31 stand-up except that uh his wife is now
102:33 sitting in the audience and he has to go
102:35 get her she doesn't want to do it and he
102:36 drags her onto the stage and the moment
102:38 they get started she doesn't miss a line
102:41 she does all the steps correct it was
102:43 just incredible they were a wonderful
102:44 couple
102:45 there's some very touching and warm
102:47 scenes from gene wilder
102:50 as uh he recollects all of his
102:52 experiences with mel brooks and
102:54 carl reiner of course figures
102:55 prominently in this as well
102:57 one of the best lines i thought i've
102:59 ever heard from him
103:00 was when he was being interviewed by
103:02 carl reiner as the 2000 year old man
103:05 and of course they sent him first to the
103:07 mayo clinic to make sure he was 2 2000
103:09 years old
103:10 and he says
103:11 well well i'm not quite 2000 years old
103:14 he says
103:15 in september 16th uh i'll be 2000 years
103:19 but i'm not quite there yet so let's not
103:20 push it
103:23 he was even funny at that level
103:25 um so carl renner asked him once he said
103:28 you know it must have been rough being
103:30 2000 years old when you first started
103:31 your life there were no medicines there
103:33 were no doctors he said what did you do
103:35 for illnesses he said what do you mean
103:37 what kind of illness have you got in
103:38 mind he says well
103:39 what if you got diarrhea what would
103:41 happen he says well you took clean
103:42 peaches
103:46 that was instantaneous out of nowhere
103:48 the man was and still
103:50 is um the fastest thinking funny man
103:55 i've ever heard so that's that's my pick
103:58 i'll have to have a look at this it
103:59 looks good oh you'll like it you'll like
104:01 it you know another quick one was joan
104:03 rivers she was really good i don't know
104:05 she was excellent she was i don't know
104:06 if she's on your list but i liked she
104:08 was really rapid she could have she
104:10 wasn't you're absolutely right in fact
104:12 someone
104:13 put an old clip of her
104:16 on the johnny so johnny carson
104:17 apparently got her started
104:19 yeah but there was a disconnect yeah
104:21 well then when she when he was retiring
104:23 she wanted him to
104:24 give her the show and he didn't so she
104:26 was right
104:28 i watched this clip of her
104:30 where she came 20 years after her first
104:32 appearance and
104:33 she was so quick and
104:35 saying funny things it was amazing
104:37 yup
104:38 totally agree cathy
104:40 sorry sorry born melvin kaminsky he's 95
104:43 years old
104:45 he was a corporal in the u.s army
104:48 served in the battle of the bulge
104:50 exactly melvin kaminsky
104:53 kathy what do you have for us so i have
104:55 some watercolors that are a virtual
104:58 exhibit and i want to read you
105:00 um
105:02 something
105:03 written by the artist
105:05 i'm a painter photographer who spent 12
105:07 years at the art students league in new
105:09 york studying with mario cooper and dale
105:11 myers i am a member of the sama gundy
105:14 club in new york city these are
105:16 representative of some of my recent
105:18 watercolor paintings that i entitled
105:20 germination
105:21 i live in fort lee new jersey with my
105:23 wife marlene bloom an accomplished
105:26 artist who paints in oil on canvas
105:29 to see my photographs please go to
105:32 depommyphotoart.com
105:35 so what you have now figured out is that
105:39 these beautiful watercolors are by dixon
105:43 and i didn't even know that he had this
105:47 talent or this hobby uh
105:50 and they're just they're just fantastic
105:52 well i love so yeah
105:55 how this art show came about and and
105:57 you're
105:58 doing watercolors
106:00 yeah well um
106:02 it all started 12 years ago no that's
106:04 not true
106:05 i was i always used to love to draw as a
106:07 kid
106:08 and i think that's what drew drew me to
106:10 uh no pun intended to the
106:12 to the visual aspects of biology i loved
106:15 all the diagrams and the colors of the
106:18 different tissues etc
106:19 um
106:20 i collected stuff out of nature and uh
106:23 used to observe them in little jars and
106:25 stuff just like other little kids did
106:27 then when i got to be an adult i reached
106:29 a point in my life when i
106:31 i needed to fill a gap that was missing
106:32 for quite a while so i decided to take
106:34 some lessons at the art students league
106:36 which is
106:37 anybody can walk in that door
106:39 pay 56 dollars for months worth of
106:43 lessons from world famous artists
106:46 and without any um
106:49 trepidations of fear of being overly
106:51 criticized because you don't know how to
106:53 do anything and i sat in mario cooper's
106:56 um
106:56 class for a month without painting
106:58 anything
106:59 i watched everybody else you sit in this
107:01 big square room and there might be 30
107:03 people in there and they're all busy
107:05 painting and they're getting paper out
107:07 and everything and i'm sitting there
107:08 like i've never seen any of this before
107:10 it's like you know entering an operating
107:12 room and you're going to do brain
107:13 surgery and then they expect you to do
107:15 it the first time you go in there you
107:16 can't do it and i see this kid six years
107:19 old sitting on my left and there's a
107:20 woman in her late 80s sitting on my
107:22 right and they're both feverishly
107:24 working away and i've got this blank
107:26 piece of paper
107:27 and mario cooper sits in the middle of
107:29 this square and he goes around and he
107:30 looks at everybody's work
107:32 he's got this little pencil in his hand
107:35 and if you've done something good
107:37 he gives you a little penciled star at
107:39 the bottom left hand corner of your
107:41 paper
107:42 and if you've done something bad he just
107:43 keeps walking he doesn't say anything he
107:46 just
107:46 keeps going and he says or he'll say
107:48 something like well i think you've done
107:50 enough painting on that one but you know
107:51 he'll move on
107:53 but he's got these he had these
107:54 wonderful insights uh which
107:57 really
107:58 were quite
108:00 applicable to lots of other things uh
108:02 and he said for instance he has the
108:04 class he is what's the darkest place in
108:06 the room
108:08 and everybody's looking around for the
108:10 darkest place in the room he says well
108:12 you don't have to look too far he says
108:13 it's actually your eyes
108:16 if you look at your eyes not the pupils
108:18 but the irises around them you can
108:21 that's the darkest place if you look
108:22 into the pupil it's even darker
108:26 so when you make your eyes and they went
108:28 on to explain how to do eyes at any rate
108:29 so i i started out painting
108:32 flowers and i you know i was very bad at
108:35 it i mean i was really bad but i must
108:37 tell you here's what kind of a teacher
108:39 he was
108:41 the first time i put paint on paper
108:44 it didn't matter what it was and i
108:46 forgot i didn't even save it he came
108:48 over to me specifically he looked down
108:51 and he put a little
108:53 penciled star at the bottom left-hand
108:55 corner of my paper
108:58 he says you're not going gonna know if
108:59 you're a painter unless you paid a miles
109:01 worth of painting
109:03 when you get to the mile
109:05 then you can look back and say well i
109:07 didn't make much of an improvement here
109:08 i think i'll take up golf
109:10 you know but but i i kept improving i
109:13 guess and um all these iterations of the
109:17 germination series
109:19 started with pictures of flowers and
109:21 those are all stamens of flowers that
109:24 morphed into these crazy
109:27 things and his wife dale meyer who took
109:29 over his class for him after he passed
109:31 away
109:32 it was a very sad event of course um
109:35 she looked down at me i was painting
109:36 flowers up to that point and all of a
109:38 sudden she looks at these things and
109:39 says
109:40 well you've certainly stepped on the
109:41 edge of a cliff now haven't you
109:45 and i
109:46 i just kept doing i'm still doing them i
109:48 i
109:49 i love
109:51 the feeling of paint on paper and the
109:53 effect that it has i don't like messy
109:55 artwork like
109:57 oils my wife loves them
109:59 they stink

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