Box jellyfish

**Box jellyfish** (class **Cubozoa**) are cnidarian invertebrates distinguished by their cube-shaped medusae. Some species of box jellyfish produce extremely potent venom: *Chironex fleckeri*, *Carukia barnesi* and *Malo kingi*. Stings from these and a few other species in the class are extremely painful and can be fatal to humans.

## Taxonomy and systematics

At least 51 species of box jellyfish were known as of 2018. These are grouped into two orders and eight families. A few new species have since been described, and it is likely that additional undescribed species remain.

Class **Cubozoa**

* Order Carybdeida
	+ Family Alatinidae
	+ Family Carukiidae
	+ Family Carybdeidae
	+ Family Tamoyidae
	+ Family Tripedaliidae
* Order Chirodropida
	+ Family Chirodropidae
	+ Family Chiropsalmidae
	+ Family Chiropsellidae

## Description

The medusa form of a box jellyfish has a squarish, box-like bell, from which its name is derived. From each of the four lower corners of this hangs a short pedalium or stalk which bears one or more long, slender, hollow tentacles. The rim of the bell is folded inwards to form a shelf known as a velarium which restricts the bell's aperture and creates a powerful jet when the bell pulsates. As a result, box jellyfish can move more rapidly than other jellyfish; speeds of up to 6 metres (20 ft) per minute have been recorded.

In the center of the underside of the bell is a mobile appendage called the manubrium which somewhat resembles an elephant's trunk. At its tip is the mouth. The interior of the bell is known as the gastrovascular cavity. It is divided by four equidistant septa into a central stomach and four gastric pockets. The eight gonads are located in pairs on either side of the four septa. The margins of the septa bear bundles of small gastric filaments which house nematocysts and digestive glands and help to subdue prey. Each septum is extended into a septal funnel that opens onto the oral surface and facilitates the flow of fluid into and out of the animal.

The box jellyfish's nervous system is more developed than that of many other jellyfish. They possess a nerve ring around the base of the bell that coordinates their pulsing movements, a feature found elsewhere only in the crown jellyfish. Whereas some other jellyfish have simple pigment-cup ocelli, box jellyfish are unique in the possession of true eyes, complete with retinas, corneas and lenses. Their eyes are set in clusters called rhopalia, located in pockets halfway up the outer, flat surfaces of the bell. Each contains two rhopalial ocelli with lenses, one directed upwards and the other downwards and inwards towards the manubrium. This enables the animal to see specific points of light, as opposed to simply distinguishing between light and dark. Box jellyfish also have twenty ocelli (simple eyes) that do not form images, but detect light and dark; they therefore have a total of twenty-four eyes. Near the rhopalia are statoliths which detect gravitational pull and help the animal to orient itself.

Box jellyfish also display complex, probably visually-guided behaviors such as obstacle avoidance and fast directional swimming. Research indicates that, owing to the number of rhopalial nerve cells and their overall arrangement, visual processing and integration at least partly happen within the rhopalia of box jellyfish. The complex nervous system supports a relatively advanced sensory system compared to other jellyfish, and box jellyfish have been described as having an active, fish-like behavior.

A fully grown box jellyfish can measure up to 20 cm (7.9 in) along each box side (or 30 cm (12 in) in diameter), and the tentacles can grow up to 3 m (9.8 ft) in length. Its weight can reach 2 kg (4.4 lb). There are about 15 tentacles on each corner. Each tentacle has about 500,000 cnidocytes, containing nematocysts, a harpoon-shaped microscopic mechanism that injects venom into the victim. Many different kinds of nematocysts are found in cubozoans.

## Distribution

Although the notoriously dangerous species of box jellyfish are largely restricted to the tropical Indo-Pacific region, various species of box jellyfish can be found widely in tropical and subtropical oceans, including the Atlantic Ocean and the east Pacific Ocean, with species as far north as California (*Carybdea confusa*), the Mediterranean Sea (*Carybdea marsupialis*) and Japan (such as *Chironex yamaguchii*), and as far south as South Africa (for example, *Carybdea branchi*) and New Zealand (such as *Copula sivickisi*).

## Ecology

### Age and growth

It has been found that the statoliths, which are composed of calcium sulfate hemihydrate, exhibit clear sequential incremental layers, thought to be laid down on a daily basis. This has enabled researchers to estimate growth rates, ages, and age to maturity. *Chironex fleckeri*, for example, increases its inter-pedalia distance (IPD) by 3 mm (0.12 in) per day, reaching an IPD of 50 mm (2 in) when 45 to 50 days old. The maximum age of any individual examined was 88 days by which time it had grown to an IPD of 155 mm (6 in).

### Behavior

The box jellyfish actively hunts its prey (small fish), rather than drifting as do true jellyfish. They are capable of achieving speeds of up to 1.5 to 2 metres per second or about 4 knots (7.4 km/h; 4.6 mph).

The venom of cubozoans is distinct from that of scyphozoans, and is used to catch prey (small fish and invertebrates, including prawns and bait fish) and for defence from predators, which include the butterfish, batfish, rabbitfish, crabs (blue swimmer crab) and various species of turtle including the hawksbill sea turtle and flatback sea turtle. It seems that sea turtles are unaffected by the stings because they seem to relish box jellyfish.

## Danger to humans

Although the box jellyfish has been called "the world's most venomous creature", only a few species in the class have been confirmed to be involved in human deaths, and some species pose no serious threat at all.

In Australia, fatalities are most often caused by the largest species of this class of jellyfish, *Chironex fleckeri*. Researchers at the University of Hawaii's Department of Tropical Medicine found the venom causes cells to become porous enough to allow potassium leakage, causing hyperkalemia, which can lead to cardiovascular collapse and death as quickly as within 2 to 5 minutes. It was postulated that a zinc compound may be developed as an antidote.

In Australia, *C. fleckeri* has caused at least 64 deaths since the first report in 1883, but even in this species most encounters appear to result only in mild envenoming. Most recent deaths in Australia have been in children, which is linked to their smaller body mass. In parts of the Malay Archipelago, the number of lethal cases is far higher (in the Philippines alone, an estimated 20-40 die annually from Chirodropid stings), probably owing to limited access to medical facilities and antivenom, and the fact that many Australian beaches are enclosed in nets and have vinegar placed in prominent positions allowing for rapid first aid. Vinegar is also used as treatment by locals in the Philippines.

The recently discovered and very similar *Chironex yamaguchii* may be equally dangerous, as it has been implicated in several deaths in Japan. It is unclear which of these species is the one usually involved in fatalities in the Malay Archipelago. In 1990, a 4-year-old child died after being stung by *Chiropsalmus quadrumanus* at Galveston Island in the Gulf of Mexico, and either this species or *Chiropsoides buitendijki* are considered the likely perpetrators of two deaths in West Malaysia. Warning signs and first aid stations have been erected in Thailand following the death of a 5-year-old French boy in August 2014. A woman died in July 2015 after being stung off Ko Pha Ngan, and another at Lamai Beach at Ko Samui on 6 October 2015. At least two deaths in Australia have been attributed to the thumbnail-sized Irukandji jellyfish. Those who fall victim to these may suffer severe physical and psychological symptoms, known as Irukandji syndrome. Nevertheless, most victims do survive, and out of 62 people treated for Irukandji envenomation in Australia in 1996, almost half could be discharged home with few or no symptoms after 6 hours, and only two remained hospitalized approximately a day after they were stung.

In Australia (mainly northern; however, box jellyfish can live in subtropical waters as far south as Sydney, with *Carybdea marsupialis* being recorded as far south as Bermagui, Sapphire Coast) the highest risk period for the box jellyfish is between October and May, but stings and specimens have been reported all months of the year. Similarly, the highest risk conditions are those with calm water and a light, onshore breeze; however, stings and specimens have been reported in all conditions.

In Hawaii, box jellyfish numbers peak approximately seven to ten days after a full moon, when they come near the shore to spawn. Sometimes the influx is so severe that lifeguards have closed infested beaches, such as Hanauma Bay, until the numbers subside.

### Protection during swimming or diving

Wearing pantyhose or full body lycra suits during diving (both by women and men, also under scuba-diving suit) is an effective protection against box jellyfish stings. The pantyhose were formerly thought to work because of the length of the box jellyfish's stingers (nematocysts), but it is now known to be related to the way the stinger cells work. The stinging cells on a box jellyfish's tentacles are not triggered by touch, but are instead triggered by the chemicals found on skin; the chemicals are not present on the hose's outer surface, so the jellyfish's nematocysts do not fire.

### First aid for stings

See also: Jellyfish#Treatment of stings
Once a tentacle of the box jellyfish adheres to skin, it pumps nematocysts with venom into the skin, causing the sting and agonizing pain. Flushing with vinegar is used to deactivate undischarged nematocysts to prevent the release of additional venom. A 2014 study reported that vinegar also increased the amount of venom released from already-discharged nematocysts; however, this study has been criticized on methodological grounds.

Removal of additional tentacles is usually done with a towel or gloved hand, to prevent secondary stinging. Tentacles can still sting if separated from the bell, or after the creature is dead. Removal of tentacles may cause unfired nematocysts to come into contact with the skin and fire, resulting in a greater degree of envenomation.

Although commonly recommended in folklore and even some papers on sting treatment, there is no scientific evidence that urine, ammonia, meat tenderizer, sodium bicarbonate, boric acid, lemon juice, fresh water, steroid cream, alcohol, cold packs, papaya, or hydrogen peroxide will disable further stinging, and these substances may even hasten the release of venom. Heat packs have been proven for moderate pain relief. The use of pressure immobilization bandages, methylated spirits, or vodka is generally not recommended for use on jelly stings. In severe *Chironex fleckeri* stings cardiac arrest can occur quickly.

### Possible antidotes in humans

In 2011, researchers at the University of Hawaii announced that they had developed an effective treatment against the stings of Hawaiian box jellyfish by "deconstructing" the venom contained in their tentacles. Its effectiveness was demonstrated in the PBS *Nova* episode "Venom: Nature's Killer", originally shown on North American television in February 2012. Their research found that zinc gluconate prevented the disruption of red blood cells and reduced the toxic effects on the cardiac activity of research mice.

In April 2019, a team of researchers at the University of Sydney announced that they had found a possible antidote to *Chironex fleckeri* venom that would stop pain and skin necrosis if administered within 15 minutes of being stung. The research was the result of work done with CRISPR whole genome editing in which the researchers selectively deactivated skin-cell genes until they were able to identify ATP2B1, a calcium transporting ATPase, as a host factor supporting cytotoxicity. The research showed the therapeutic use of existing drugs targeting cholesterol in mice, although the efficacy of the approach has not yet been proven in humans.