recursal/Fanatic-Fandom huggingface dataset:
Liberty Sunshine

**Liberty Sunshine** (リバティーサンシャイン) is a coord that belongs to the live genre and the brand SHINING DIVA. It first appeared in episode 40 of Waccha Primagi! worn by Jennifer Sumire Sol.

### Game

It is an ultra rare coord that belongs to the live genre and the brand SHINING DIVA. It first appeared in Chapter 6: Queen Jennifer! The Primagi Grand Festival! as a part of the Chapter 6 Coords Collection 1.

## Appearance

### Dress

A shimmery sweetheart mini-dress with vertical pearl chains covering it. A metallic fringe is sewn diagonally around the chest and skirt, and hanging from the back is a red cape-like layer with a dark red and gold flame design and trim of gold shimmery feathers. Around the waistband are white and gold pearl chain loops decorated with orange and red gems. Above the chains is a large gem on a gold sun base. The bottom of the skirt is trimmed with glittery silver ruffles. A sequined jacket is worn over the dress with a silver lining on the torso and a red lapel decorated with gems and gold designs. Sewn on each side is an orange feather cluster tipped in gold, and hanging from the back are gold sequined strips. The layered sleeves have a silver cuff lined in gold, adorned by several gems. A gem choker with gold detail is included with pearl chains looped beneath it.

### Shoes

White sandals with a silver chunk heel and straps over the foot bound together by pearl chains. The gems going up the center come in blue, green, yellow, and red, with the red gem on a gold sun attached to the pearl anklet. On top of this are orange gradient feathers tipped in gold. The legs are covered by two different pieces, the right leg has a sequined piece with the knee cut out and two pearl chains at the thigh. The left leg is shimmery with a silver ornate piece wrapped around it with gems.

### Accessory

A silver tiara with a gem sun in the middle. Decorating it are gold-tipped feathers and five-pointed rainbow gem shards.
Asaka Sara

Template:Infobox character
Template:Nihongo is a supporting character in *KiraKira☆Pretty Cure A La Mode* and member of the band Wild Azur. She first appears in episode 1 as a cameo but made her official debut in episode 3. She plays the drums.

## Bio

### Appearance

She has black hair cut at an angle with a streak of pale grey off-center of her bangs, and she has sharp brown eyes. She usually wears a mauve hat with a black stripe around the middle and fuchsia earrings. Her lips are more pronounced than most characters.

Her normal attire consists of a grey long sleeved shirt beneath an angular cut grey T-shirt, grey pants with a crimson belt, an a pair of light grey and white sneakers.
Lemonade Idol Coord

**Lemonade Idol Coord** (レモネードアイドルコーデ) is a premium-type coord that is Brandless. In the Pretty Rhythm series, it is composed of star-type prism stones. It first appeared in episode 31 of Pretty Rhythm Dear My Future worn by So Min.

### Game

The coord is composed of star-type normal prism stones that first appeared in Season 11. In the PriChan arcade, the coord is a premium-type Pri☆Chan rare coord that is Brandless.

## Appearance

### Tops

A white buttoned top with ruffles on each side of the chest and a short red tie. A low-cut goldenrod vest is worn over this with four gold buttons and white lining. White wrist cuffs with gold buttons are included.

### Bottoms

Puffy goldenrod shorts with ruffled trim on the leg holes. Each layer is lined in white.

### Shoes

Short goldenrod boots with a black sole. The top is lined in white.
Manamana Lemon

**Manamana Lemon** (マナマナれもん) is a coord that belongs to the live genre and the brand Radiant Abyss. It did not appear in the anime.

### Game

It is a super rare coord that belongs to the live genre and the brand Radiant Abyss. It first appeared in Primagi Studio Chapter 4: Carrots for Waiting! Here Comes Carron! as a part of the Primagi Lottery.

## Gallery

### Official Coord

* 2

*Manamana Lemon Hair Accessory*
Little Princess Pink

**Little Princess Pink** (リトルプリンセスピンク) is a coord that belongs to the live genre and the brand Princess Magic. It did not appear in the anime.

### Game

It is a super rare coord that belongs to the live genre and the brand Princess Magic. It first appeared in Primagi Studio Chapter 3: Swee~t Primagi! Hanitan's Debut hani~! as a part of the Promotional Coords.
Solaine

Template:Infobox character

Template:Nihongo is the boss of the pâtisserie "MON TRESOR" who scouted and educated Kirahoshi Ciel about becoming a top pâtissier.

## Bio

### Appearance

Solaine is a French woman who has ivory-colored hair. She wears framed sunglasses, pink lip gloss and yellow teardrop earrings. She normally is in a pale baby-blue blouse and a pair of black trousers, as well as black flats. A long blue scarf with red stripes is around her neck.

### Personality

Solaine is dedicated to the qualities of desserts and is rather picky about sweets at any stores she visits, and can be judgmental and critical if her standards are not being met, as seen in episode 37. However, she does have an eye for noticing when a person does have talent, such as training Ciel to become the very best.

### Relationships

* **Kirahoshi Ciel** - Her former student. She didn't like the idea of Ciel living at Ichigozaka, but eventually came to accept Ciel's decision.

## History

Prior to the season, Solaine was searching for a new partner to run her shop. She came across Jean-Pierre Zylberstein, but Jean-Pierre refused causing them to argue. Right after Jean-Pierre walked away from her, she noticed that Kirahoshi Ciel had a talent for cooking and scouted her. With Ciel, MON TRESOR gained popularity and the Paris economy soared, until Ciel left a note saying she wanted to travel the world.

After spending some time searching for Ciel, Solaine first appeared at a live demonstration at the Halloween Party, criticizing the pumpkin dessert Ciel had finished. She then inspected the shop Ciel owned and deemed it as too small for a genius like Ciel and that Ichigozaka was limiting Ciel's abilities. She then handed the contest flyer to Ciel, as a way to push her to join it.

The next day, Solaine was surprised Ciel had made her a hamster pumpkin pudding that was created by Ciel and the five other girls. Upon tasting it, she recalled her youth in rural France. After listening to Ciel's speech on the importance of friendship, Solaine was deeply impressed, invited Ciel's friends to join Ciel's company, and finally acknowledged Ciel's growth when she was away.
Horaemon Wiki

**Welcome to the Horaemon Wiki!**
Horaemon (Japanese: ホラえもん) is a collective parody series. It parodied I Dream of Jeannie (1965-1970), Jeannie (1973), Winsome Witch (1965-1966) and Wizards of Waverly Place (2007-2012) under the pen name of Fujiko H. Fujio (Japanese: 藤子・H・不二雄; Hepburn: Fujiko eichi fujio), which was not used by any author of the Fujiko Fujio family. It is produced by Shin-Ei Corporation.

We're a collaborative community website about Horaemon that anyone, including you, can build and expand. Wikis like this one depend on readers getting involved and adding content. Click the "ADD NEW PAGE" or "EDIT" button at the top of any page to get started!

## Important articles

PlaceholderA page about your topicPlaceholderA Main CharacterPlaceholderThe First EpisodePlaceholderAn Important LocationPlaceholderA Key EventPlaceholderA Crucial Item

*Need help building out this community?*

* Rules of this wiki
* Getting Started
* How to Contribute
* Managing your new community
* Guides
* All Help articles

You can also be part of the larger Fandom family of communities. Visit Fandom's Community Central!

*Community Founders*: Write a good and paragraph-length description for your welcome section about your topic. Let your readers know what your topic is about and add some general information about it. Then you should visit the admin dashboard for more tips.
Wizards of Atsuko Place

***Wizards of Atsuko Place*** is an implementation of *Wizards of Waverly Place* for *Horaemon*. It stars Atsuko Kagami, Moko-chan and Sazae Fuguta (from *Sazae-san*) as three wizard siblings with magical abilities competing to win sole custody of the family powers.

The theme song for English is "Everything Is Not What It Seems" performed by Selena Gomez and the theme song for Japanese is "Yume wo Kanaete Horaemon" (as it is integrated to the main *Horaemon* package).

## Theme song lyrics

* English
* Japanese (romaji)
* Japanese (kana)

Well you know everything's gonna be a breeze

That the end will no doubt justify the means

You can fix any problem with the slightest ease

Yes, please..

Well you might find out it'll go to your head

When you write a report on a book you never read

With the snap of your fingers you can make your bed

That's what I said

Everything is not what it seems!

When you can get all you wanted in your wildest dreams,

You might run into trouble if you go to extremes,

Because everything is not what it seems

Everything is not what it seems!

When you can have what you want by the simplest of means

Be careful not to mess with the balance of things

Because everything is not what it seems!

Kokorononaka itsumo itsumo egai teru, (Egai teru!)

Yume o noseta jibun dake no sekai chizu, (Tachakonta ~!)

Sora o tonde jikan o koete tōi kuni demo,

Doawoakete hora ikitai yo imasugu. (Dokodemodoa ~!)

Otonaninattara wasure chau no ka na?

Son'na tokiniha omoidashite miyou…

Sharararara, boku no kokoro ni,

Itsu made mo kagayaku yume,

Horaemon, sono poketto de kanae sasete ne!

Sharararara, uta o utaou,

Min'nade sā tewotsunaide,

Horaemon, sekaijū ni yume o sō afure sasete!

心の中 いつもいつもえがいてる、（えがいてる！）

夢をのせた自分だけの世界地図、（タチャコンタ～！）

空を飛んで時間を越えて遠い国でも、

ドアをあけてほら行きたいよ今すぐ。（どこでもドア～！）

大人になったら忘れちゃうのかな？

そんな時には思い出してみよう…

シャララララ、僕の心に、

いつまでもかがやく夢、

ホラえもん、そのポケットでかなえさせてね！

シャララララ、歌をうたおう、

みんなでさあ手をつないで、

ホラえもん、世界中に夢をそうあふれさせて！
Sonobe Kei

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. He's the founder and oldest member of the band Wild Azur. He plays the bass guitar.

## Appearance

Kei wears a gray shirt with a black cat on it. He also wears a yellow jacket, purple pants, and brown shoes.

## Gallery

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Nakamura Midori

Template:Infobox character
Template:Nihongo is a minor character from *KiraKira☆Pretty Cure A La Mode*. She first appears in episode 9. She works as a nursery school teacher in Ichigozaka.

## Bio

### Appearance

Midori has tanned skin and big, moss-colored eyes.She has long straightened brown hair with an off-center split in her bangs, and she wears a white headband. Over her ears are a pair of big round glasses.

Normally, she wears a white sweater with a turquoise ribbon at the neck, paired with a pale beige skirt, black socks, and a pair of pink and white sneakers. Over her outfit is a pastel pink apron with pink, blue, and green pockets.

## Gallery

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Little Baron

**Little Baron** (リトルバロン) is a coord that belongs to the stylish genre and the brand Radiant Abyss. It did not appear in the anime.

#### Game

It is a rare coord that belongs to the stylish genre and the brand Radiant Abyss. It first appeared in Chapter 2: An Excited Lemon! as a part of the Chapter 2 Coords.

## Appearance

### Tops

A white top and grey vest worn with a dark grey blazer over it. The blazer has white lining to accent its cuffs, while the black lapel has white and grey lining and four buttons on the torso. The bottom is trim with ruffles. At the neck is a cerulean cravat with bow.

### Bottoms

Dark grey shorts with black an white leg cuffs. White and grey lines the middle area and each hip, and going down each leg are vertical grey stripes. The string laced through the middle is grey.

### Shoes

Dark grey boots with black sole and grey shoelaces, accenting the stripe around the top of the shoe and white stockings. On the side of the stocking is a dark grey buckled strap.

### Accessory

Dark grey cap with grey string laced through it and a band of cerulean around the middle.
Mitsuoka Junko

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. She is one of Ichika's classmates.

## Bio

### Appearance

Junko is a young girl with short brown hair worn in two pigtails, and she has thick bangs mostly brushed aside. She has brown eyes.
Normally she is only seen in her school uniform.

### Personality

Junko is known to be easy going and laid back.

## Trivia

* She shares her voice actress with Nono Hana from *HUGtto! Pretty Cure*.

## Gallery

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Blue Sky in the Pocket

"**Blue Sky in the Pocket**" (青い空はポケットさ *Aoi Sora wa Poketto Sa*) is an ending song that was first used for the first three years of *Horaemon* (from 1979 to 1981). The ending footage featured Doraemon characters for 1979 and Candice (from *Candy Candy*) and Atsuko Kagami (from *Himitsu no Akko-chan*) for 1979 and 1980.

The remastered release of *Invisible House For Sale* displays images of Atsuko Kagami dressed up like *Sofia the First* characters accompanied by credits and this song. Similarly, the remastered versions of *The Blind Date* and *The Commercial* of *Atsuko the Jeannie* used images of Candice and Atsuko Kagami cosplaying as League of Legends characters, replacing the footage featuring Doraemon characters.

## Used in:

* 1979 - Atsuko the Jeannie
* 1979 - I Dream of Candy
* 1980 - I Dream of Candy
Lucky Coord Mates

**Lucky Coord Mates** (ラッキーコーデメイツ) is a coord that belongs to the live genre and the brand Princess Magic. It did not appear in the anime.

### Game

It is a super rare coord that belongs to the live genre and the brand Princess Magic. It first appeared in Primagi Studio Chapter 8: Awaken, Himeme! The Miraculous Oshare Princess! as a part of the Promotional Coords.
I Love Kuromi

**Rarity** | Super Rare

**I Love Kuromi** (クロミだいすき) is a coord that belongs to the live genre and the brand Radiant Abyss. It did not appear in the anime.

### Game

It is a super rare coord that belongs to the live genre and the brand Radiant Abyss. It first appeared in Chapter 6: Queen Jennifer! The Primagi Grand Festival! as a part of the Limited-Time Coords.

## Appearance

### Tops

A grey vertical stripe top with black straps going around the torso and shoulder, connected by a chain of white, purple and black spheres. Grey ruffles go around the top of the chest and off-shoulder sleeve, which have accents of purple. A solid grey band goes down the chest with images of Kuromi and black hearts, with a strip of purple and white tulle on each side. The black and white scalloped fabric on the neck has a purple bow adorned by a pink and black skull. White gloves are included, with a split cuff based on the top with a purple woven band around the wrist. Multiple rings are included.

### Bottoms

A black pannier with the Kuromi fabric and tulle design from the top going down the middle. On each lower-side of the skirt is the skull bow, and the bottom of the skirt is trim with ruffles over a white fluffy petticoat. Around the skirt hem is a purple wavy stripe. Attached to the waist is a purple and black frilly purse with off-white, purple and black pom-pom on the strap. Kuromi's face is drawn on the front.

### Shoes

Purple shoes with pom-pom sewn to the strap and a black sole. White tube socks are included, with a black ribbon design and Kuromi's head on the side. The grey striped cuff has a woven band around the middle. On the thigh is a spherical chain.

### Accessory

A grey woven hat with a purple strap around the middle. A chain of spheres circles the bottom with a glittery Kuromi head on the front.
Xu

**Capital City Population**: approx. 20000

**Political View**: Emperor. The Xu Empire is currently being ruled by Emperor Ryuzaki. He is known to have 2 sons.

**Races**: All races can be found here but mostly Humans.

**Main Trade**: As the Capital City of the Empire, it has almost everything but is mostly known for it's army power.

**City Attractions**:

* Xu Tower
* Xu Waterfall
* Best army barracks

**Defenses**: The city is protected by multiple layers of stone walls and is protected by the Empire Guards.
Izumi Wataru

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. He is very good at sports and Ichika's classmate.

## Appearance

Wataru has brown spiky hair with brown colored eyes. He is seen usually with a bandage on his right cheek and wearing his school uniform.

## Trivia

* He shares his first name with Kaido Wataru, a minor character and Minami's brother from *Go! Princess Pretty Cure*.

## Gallery

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I Love My Melody

**Rarity** | Super Rare

**I Love My Melody** (マイメロディだいすき) is a coord that belongs to the live genre and the brand Cherry Sugar. It did not appear in the anime.

### Game

It is a super rare coord that belongs to the live genre and the brand Cherry Sugar. It first appeared in Chapter 6: Queen Jennifer! The Primagi Grand Festival! as a part of the Limited-Time Coords.
Little Baron Navy

**Little Baron Navy** (リトルバロンネイビー) is a coord that belongs to the casual genre and the brand Radiant Abyss. It did not appear in the anime.

### Game

It is a rare coord that belongs to the casual genre and the brand Radiant Abyss. It first appeared in Primagi Studio Chapter 4: Carrots for Waiting! Here Comes Carron! as a part of the Studio Chapter 4 Coords.
Nakano Hiroki

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. He is Ichika's classmate.

## Appearance

Hiroki has messy light brown hair with the same colored eyes. He is seen usually wearing his school uniform with a red shirt underneath.

## Personality

Hiroki is known to be make any mood cheerful and loves to play basketball.

## Gallery

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Kagurazaka Risa

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. She is one of Ichika's classmates.

## Bio

### Appearance

Risa has chin length brown-auburn hair that flares out on end. Her eyes are brown and she is normally seen in her school uniform.

### Personality

Risa is a cheerful young girl who tends to fangirl a lot.

## Trivia

* Risa shares her name with Shibata Risa from *Heartcatch Pretty Cure!*.

## Gallery

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Kirakiraru Thieves

File:KKPCALM 01 Kirakiraru Thieves.png  The Template:Nihongo were a group of evil and mischievous fairies who were the first antagonists in *KiraKira☆Pretty Cure A La Mode.* Their main goal is to steal the Kirakiraru found in the world's sweets to become powerful. They wear a red belt with a buckle that has a black star on it. Using said belt's dark energy, they can drain the Kirakiraru from sweets or desserts, which turn the sweets black.

The thieves use their favourite sweets to drain the Kirakiraru which differs from member to member - Gummy desires the Kirakiraru from cakes, while Pulupulu seeks the Kirakiraru from puddings. If they swallow the Kirakiraru, they can transform into bigger and more powerful forms of themselves depending on how much they use.

In episode 11, the thieves were all purified by the Cures, with the star-shaped buckles shattered. In episode 12, Gummy reveals to the Cures that he and the other fairies who once comprised the group were given the belts by Julio, putting them under his control. In episode 21, it is revealed that the purified thieves helped the missing fairies reunite with Pekorin and Chourou.

## Trivia

* Gummy is the only member of the Kirakiraru Thieves to have his own unique voice actor.

## Gallery

*Main Page: Kirakiraru Thieves/Image Gallery*
Usami Genichirou

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. He is the father to Usami Ichika, and husband to Usami Satomi.

## Personality

He works at a karate dojo and can easily tell if Ichika is feeling troubled by just looking at her.

## Relationships

* **Usami Ichika** - His daughter, whom he loves dearly. He takes care of Ichika while her mother is traveling around the world as a doctor.
* **Usami Satomi** - His wife.

## Gallery

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Grocery Shop Owner

Template:Infobox character
The Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. He is in charge of the PR for Ichigozaka's shopping district.

## Appearance

He is a slightly aged man with short gray hair, but he normally wears a blue cap. He wears a faded mint green-colored jacket with a white shirt underneath. He also wears khaki pants, black and white sneakers, and a navy blue apron.

## Personality

He is said to be a friendly man who helps with the shopping district's PR.

## History

In episode 6, he mistakes Kenjou Akira for Ichika's boyfriend. In the same episode, he also tells Akira how he has known Ichika back when she was a baby.

In episode 13, he enlists the help of Ichika, Himari, and Aoi to promote the city's shopping district. Himari is left in charge of interviewing people on the street, but her shyness gets the better of her. However, he later compliments Himari after her report had become popular on the internet.

## Relationships

* **Usami Ichika** - He has known Ichika since she was a baby, and is friends with her.

## Gallery

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Marching Hanitan

**Marching Hanitan** (マーチングはにたん) is a coord that belongs to the live genre and the brand Eternal Revue. It did not appear in the anime.

### Game

It is a super rare coord that belongs to the live genre and the brand Eternal Revue. It first appeared in Primagi Studio Chapter 8: Awaken, Himeme! The Miraculous Oshare Princess! as a part of the Primagi Lottery.
Horaemon no Uta

"**Horaemon no Uta**" is the official opening theme song for the Japanese version of Horaemon. It was first used on 5 January 1979 as the opening theme for Atsuko the Jeannie's *Helen the Troy*. It parodies *Doraemon's Song* of 1979.

## Lyrics

* Kana
* Romaji

こんなこと良いな、てきたらいいな、

あんあ夢、こんな夢、いっぱいあるけど。

みんな、みんな、みんな、叶えてくれる、

不思議なポッケで叶えてくれる。

空を自由に飛びたいな…

「ハイ! タチャコンター」

アン、アン、アン、とっても大好き… ホラえもん。

アン、アン、アン、とっても大好き… ホラえもん。

Kon'na koto yoi na, dekitara ī na,

An a yume, kon'na yume, ippai arukedo.

Min'na, min'na, min'na, kanaete kureru,

Fushigina pokke de kanaete kureru.

Sora o jiyū ni tobitai na…

'Hi! Tachakontā'

An, an, an, tottemo daisuki… Horaemon.

An, an, an, tottemo daisuki… Horaemon.

## Versions

* **5 January 1979 - 24 December 1994**: Kumiko Ōsugi
* **March 1982 - April 1982**: Regal Children's Chorus
* **25 December 1994 - 31 October 1997**: Satoko Yamano
* **11 November 1997 - 31 October 1999**: Vienna Boy's Choir
Usami Satomi

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. She is the mother to Usami Ichika, and wife to Usami Genichirou.

## Bio

### Appearance

Satomi resembles her daughter with long curly auburn hair worn in a low ponytail. She has red eyes.
Normally she wears a mauve top with denim pants and a pair of lavender and white sneakers. Over this is a white labcoat with a stethoscope around her neck.

### Personality

A bighearted lady, Satomi travels to small villages all over the world as a doctor. Also as a loving mother, she often makes sweets with Ichika as a means to give her the most love as possible during their limited time together.

## Relationships

* **Usami Ichika** - Her daughter. Ichika sees her mother as a heroine who travels the world to help people. Satomi regularly sends her letters and packages with handmade sweets. She sometimes regrets not being there for Ichika, but Ichika assured her that she's okay.

* **Usami Genichirou** - Her husband.

## Gallery

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Diable

Template:Infobox character
Template:Nihongo is a villain from *KiraKira☆Pretty Cure A La Mode*. It is a demonic dog-like spirit who wants Kirakiraru to regain its true form. It was defeated by the Cures in episode 36 and then absorbed into Grave's car after retreating.

## Appearance

Diable is a dark blue wolf with pointy triangular ears, a jet black star with red borders on his forehead, a dark crimson orb adorned with demon wings around his neck and sharp claws.

## History

Diable first appeared in episode 33 after following the Cures from the past. He tells the Cures to give him the kirakiraru, since he needs it to regain his normal form. The Cures refuse and the fight breaks out, with the Cures emerging victorious. After suffering defeat, he returns to Grave and Elisio's lair to rest.

He appears in episode 34 still resting, but this time he sends out 3 smaller fox like sprits to make fairies and animals hate each other.

In episode 35 he recovers from his previous defeat and now resembles a wolf like creature. Grave asks him if he would be his servant, but Diable refuses, saying he has no intention of working with a peasant like him. Diable attacks a sweets presentation, making attendees turn against Himari and Aoi, but he again ends up defeated later by the Cures, thus again returning to the lair to rest.

In episode 36 he finally regains his humanoid form after recovering and leaves to exact revenge on the Cures, but this time Grave takes major interest in him. Diable attacks the Cures and manages to stain them in darkness to immobilize them, but Cure Whip breaks out of it, followed by other Cures. Together they overpower him and he returns to the lair to rest again. This time however Grave, with the intent of using his power, arrives with his Gravemobile and runs him over.

In episode 37 Grave is seen upgrading his Gravemobile with Diable's powers and is eager to give it a "test ride".

In episode 40, he appears to have been absorbed into one of Elisio's cards along with Grave and his car after Grave is defeated by the Cures' hands.

In the finale, after being released from the cards of Elisio, he reincarnates into a puppy and is seen accompanied with a reincarnated Noir and Lumière a few years after the Cures’ final battle.

## Abilities

Diable possessed a power to change people's emotions by staining the kirakiraru in their hearts to dark. Diable also had the power to come back stronger than before after suffering defeat at the hands of Cures.
In his humanoid form, he could use his darkness to immobilize the Cures.

## Trivia

* Elisio remarked that looking at Diable was almost like staring into Noir's heart.

## Gallery

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Mizushima Mitsuyoshi

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. He works for "Tategami Konzern" and serves as Aoi's butler and caretaker.

## Appearance

He is usually seen in the typical butler attire, consisting of black tails, slacks, and shoes, a white shirt with a gray bow tie and vest, and white gloves. He has short black hair and blue eyes, as well as glasses.

## Personality

Mitsuyoshi is highly professional and responsible as a butler. He, however, also came off as conservative, believing that Aoi, being the heiress of her family business, shouldn’t be playing rock music. Deep down, Mitsuyoshi does greatly cares for Aoi, and is respectful of her ambition as a rock star.

## History

Before the beginning of the series, Mitsuyoshi used to be an orphan, until he got adopted by the Tategami family. Since then, he grew up with Aoi and formed a close bond with her.

He first appears in episode 14, making a sudden appearance at Aoi's concert with Wild Azur. He leaps onto the stage and picks up Aoi before carrying her away, leaving her guitar behind. Later on, the other Cures investigate why he did that and learned that Aoi was born into a rich family and Mitsuyoshi wants her to quit music and the patisserie to become a more refined lady, much to Aoi's objections. During the dinner party, he slowly starts to warm up to Aoi's love for playing rock music when her band makes a surprise appearance. However, Julio crashes the party and steals the Kirakiraru of her bandmates and the guests, including Mitsuyoshi. During that battle, it was shown that he actually enjoyed Aoi's song, hence why his Kirakiraru wasn't exactly stolen, and Gelato uses its strength to overpower Julio. After the guests had their Kirakiraru restored, Aoi convinces Mitsuyoshi to allow her to keep going to the KiraKira Patisserie and Wild Azur, and he agrees, as long as Aoi continues to do well in school.

In episode 42, at Wild Azur’s farewell concert, he accompanied his boss, who then entitled him as the successor of Tategami Konzern, much to his surprise.

## Relationships

* **Tategami Aoi** - Mitsuyoshi grew up with Aoi, thus they have a relatively close relationship. At first, he pretended to object Aoi from pursuing her music career, but when he was unconscious, the ripples of Soul Believer remained in his Kirakiraru, hinting his hidden support towards her, and after regaining consciousness, he permitted her.

## Trivia

* He is the third butler serving for a Cure, preceded by Jii-ya/Sakamoto, who serves Minazuki Karen from *Yes! Pretty Cure 5* and *Yes! Pretty Cure 5 GoGo!*, and Sebastian, who serves Yotsuba Alice from *Doki Doki! Pretty Cure*.
* He shares his voice actor with Nikaidou Takuya, a minor character from *Doki Doki! Pretty Cure.*

## Gallery

Template:Scroll box
Template:KKPCALM characters
Chourou

Template:Infobox character
Template:Nihongo is a wise old fairy from the *KiraKira☆Pretty Cure A La Mode* series. He lives on Ichigo Mountain. In order to please everyone, he practices making sweets with his disciples. However, after being caught in a cream explosion, his body takes the appearance of a ghost. He often asks the Cures to protect the sweets from those who aim to steal the Kirakiraru. He ends his sentences with "~jaba."

## Bio

### Appearance

Chourou has purple arms and shirt and tail. He also has a white beard that covers most of his face so his eyes cannot be seen, though it is shown that his eyes are actually brownish red. For the majority of time, he carries a yellow umbrella with him. After regaining his body, he appears to have short stubby legs.

As a human, he appears to be a tall older-looking man with white puffy hair and a mustache and brownish-orange eyes. He wears a purple suit with an dark orange ascot and white gloves. He wears the same thing on top of his head as he did as a fairy.

### Personality

Despite his old age, Chourou is still active. He cares for his apprentice a lot.

## History

In episode 1, he is briefly seen defending the inhabitants of Ichigo Mountain from the cream explosion that rained down upon Ichigozaka, causing visions of certain sweets for the five soon-to-be Cures. His voice could be heard within the box toward the end of the episode.

In episode 2, after the box is opened and turned into the full-sized KIRAKIRA Patisserie, he is seen hiding within the stone oven. After seeing him in it, Ichika promptly closes the door and panics. He then passes through the door and after he introduces himself, he explains the story of the legendary patisserie Pretty Cure to Ichika.

In episode 8, he transforms into a human in front of the Cures for the first time, but he still can't exit from the KIRAKIRA Patisserie.

In episode 39, he gathers with other fairies to hold a council meeting regarding how to face Noir's threat.

In episode 49, his body has become gigantic and gone on a rampage after absorbing too much Kirakiraru. However he is saved by the Cures and able to return to his body. A few years later, he is shown to have opened a sweets shop with Pekorin.

## Abilities

Because of his ghostly appearance, he can float through walls and other solid substances. Pekorin can also go through him. In episode 8, it is revealed that Chourou can transform into a human.

## References

Template:KKPCALM characters
Template:Mascots
Crystal Animals

File:KKPCALM 32 Crystal animals.png
The Template:Nihongo are small creatures that appear in *KiraKira☆Pretty Cure A La Mode*. Each of the Cures owns one.

After resonating with each Cures' resolve and honest emotions they evolved from spheres into crystal statues. Inserted into the Kirakiraru Creamer, they come alive and turn into large mounts for their respective Cures.

After episode 32, they are able to move on their own and act like little companions.

## Appearance

As a crystal statue, the animals are pale blue and resembles a cup with jewel designs on their detailed body.

In mount form the animals grow larger and gain crystal paws, tails or tail details, a unique gem necklace on a frilly collar, and a frilly saddle piece.

**Bunny**- Ichika's Crystal Animal, A small pale pink bunny with red eyes. On its head is a crystal bow with a strawberry gem on it. The sound it makes is "*pyon*", which is the Japanese onomatopoeia for hopping.

**Squirrel** - Himari's Crystal Animal, A light yellow squirrel with brown eyes. It has a crystal marking on its head and a crystal collar with two cherries attached to it. The sound it makes is "*kururu*", a reference to how squirrels' tails curl.

**Lion** - Aoi's Crystal Animal, A pale blue lion with a light blue mane and tuft of fur on its tail. It has blue eyes and wears a golden crown. The sound it makes is "*gao*", the onomatopoeia used for roaring.

**Cat** - Yukari's Crystal Animal, A lilac cat that has slanted purple eyes. It wears a lavender ribbon around its neck and a gem with a light purple ribbon. The sound it makes is "*nya*", the onomatopoeia used for meowing.

**Dog**  - Akira's Crystal Animal, A pale burgundy dog that has brown eyes, pointed ears, and a fluffy tail. It wears a red pointed collar or bow with a square-shaped crystal in the center. The sound it makes is "*wan*", barking.

**Pegasus** - Ciel's Crystal Animal, A pale mint pegasus with a crystalline mane, two small wings, and a sprig of holly on its left ear. Around its neck is a pale green and white collar. The sound it makes is "*patata*", likely imitative of flapping wings.

## Trivia

* In KKPCALM47, the Crystal Pegasus is able to recognize Ciel in her fairy form.
* The fate of the Crystal Animals remains unknown after the end of KiraKira☆Pretty Cure A La Mode.
Prince Nata

Template:Infobox character
**Template:Nihongo** is a minor character who appears in *KiraKira☆Pretty Cure A La Mode**.* He is the prince from the Kingdom of Confetto.

## Personality

Nata has flamboyant mannerisms in spite of his royal title. He is also too lazy to participate in hard labour such as running and doing karate even when he is the one who issued the challenge.

Because of him being lovesick towards Yukari, he is gullible enough to fall for her fickleness, and is completely oblivious to Akira being a girl.

He has a habit of using English phrases in his sentences.

## Relationships

**Kotozume Yukari** - Upon meeting Yukari, he falls for her at first sight and even going as far as challenging Akira as a means to win her over, totally unaware that she is toying with his feelings. However, he still accepts her rejection before he departs Ichigozaka for his kingdom.

## Trivia

* Prince Nata was designed by Kawano Hiroyuki, the animation director of *KiraKira☆Pretty Cure A La Mode* episode 25.

## Gallery

Template:Scroll box
Template:KKPCALM characters
Kotozume Shino

Template:Infobox character
Template:Nihongo is a minor character in *KiraKira☆Pretty Cure A La Mode*. She is Yukari's grandmother, who is a tea ceremony expert living in a tea ceremony house.

## Personality

Shino is a refined person and a highly respected expert on tea ceremonies.

## History

Before the course of the series, Shino has been teaching Yukari about the arts and manners of tea ceremonies.

She first debuts in episode 16, teaching the girls how to behave properly during tea ceremonies in an elegant manner.

## Relationships

* **Kotozume Yukari:** Her granddaughter whom she taught the basics of the tea ceremony and her own expertise. She is loving and completely supportive of Yukari pursuing any hobby. Yukari used to be averagely talented in everything, but did not enjoy doing anything. Having no goals and expectations placed on her only contributed to Yukari's anxiety in her childhood. When Yukari went missing, she was extremely worried, until she found out that Yukari managed to return safely and was relieved. She also appreciates Yukari's baking skills.

## Trivia

* She speaks using Hannari and Kyo words.

## Gallery

### Official art

KKPCALM Kotozume Shino profile Asahi 2.pngAdditional art from TV AsahiKKPCALM-concept art 2.11-Kotozume Shino.pngConcept art from the bluray art gallery
### Screenshots

KKPCALM16 - Rio and Akira proper eating.pngKKPCALM29-Yukari's grandma asking her a favor.pngShino asking Yukari for a favorKKPCALM29-Yukari's grandma waiting for Yukari.pngShino waiting for YukariKKPCALM29-Yukari's grandma tells Akira Yukari is missing.pngShino telling Akira worriedly that Yukari has gone missingKKPCALM29-Yukari's grandma talking about Yukari over macarons.pngShino appreciating Yukari through tasting macaronsKKPCALM48-Bibury tells Shino those girls are Precure.pngShino is told the girls saving everyone are Pretty Cures
Atsuko Kagami

**Atsuko Kagami** (加賀美あつ子 *Kagami Atsuko*), known as **Akko** (アツコ) for short, is the lead of Akko-chan's Got a Secret! and an occasional part of Akatsuka's Star System.

She is notable for being an early example of a magical girl, and one of Akatsuka's few shojo manga heroines after his switch to a more gag-centric career. Her personality and details are known to significantly be altered through each adaptation, to adjust to the settings of each new decade and to put in newer trends.

She is one of the first characters to appear in *Horaemon*. It first appeared in November 1978's *Akko at the Surfing Club*.
Three-star Kitty

Template:Infobox character
Template:Nihongo is a minor character from *KiraKira☆Pretty Cure A La Mode*. He is introduced in episode 5 as a cat who brings good fortune to sweets shops he visits.

## Appearance

A large, grey and white bicolor cat with three white stars on his forehead (hence his nickname).

## Personality

Although famous in Ichigozaka for supposedly bringing good fortune to sweets shops, he is notoriously picky in who he allows to pet him. Yukari's ability to tame anyone is shown by Three-star Kitty instantly running up to her and lying down on his back to be petted. Ichika's attempts to pet him keep failing comically.

## History

He is seen briefly in episode 1, but is first introduced in episode 5. In episode 8 he is shown to approve of KIRAKIRA Patisserie, thereby (supposedly) bringing good fortune on it.

In episode 34, Three-star Kitty is revealed to be the leader of the local cats. After Yukari (transformed into a cat herself) tames two of his underlings, he asks her to represent the cats in their quarrel with the fairies. With help from Kirarin she succeeds in getting the cats and fairies to make up, but transforms back into a human during the battle against Diable. Afterwards Three-star Kitty is seen sulking because he thinks cat Yukari has disappeared like she threatened, but he is cheered up by Kirarin.

Three-star Kitty plays a big role in the battle against Grave in episode 40. As leader of the cats, he leads the local animals to assist the fairies in making sweets (and kirakiraru) to help the Cures to free Ichigozaka from Grave. When they succeed in making the sweets, he and Pekorin personally bring a shortcake to Cure Whip.

In episode 45, Emiru remarks that Three-star Kitty hasn't been around much lately, and one of her friends wonders if he just left without saying anything.

## Relationships

* **Kotozume Yukari** - She appears to be the only one that he allows to pet him, even running up to her to be petted as soon as he spots her.

## Gallery

Template:Scroll box

Template:KKPCALM characters
1979

The **1979 season** marked the first use of Horaemon no Uta, first used in Atsuko the Jeannie's *Helen of Troy*.

## Atsuko the Jeannie

* Helen of Troy - 5 January 1979
* The Sailors - 31 January 1979
* The Kid Brother - 1 April 1979
* The Blind Date - 21 April 1979
* The Commercial - 8 October 1979
* Don Juan - 31 October 1979
* The Dog - 1 November 1979
* The Jinx - 15 November 1979
* The Wish - 29 November 1979

## Candy Jeannie

* Happy Anniversary - 3 March 1979
* Always on Sunday - 5 March 1979
* My Master, the Rich Tycoon - 7 March 1979
* My Master, the Rainmaker - 9 March 1979
* My Wild Eyed Master - 11 March 1979
* What's New, Poodle Dog? - 13 March 1979
* The Fastest Gun in the East - 15 March 1979
* How to Be a Genie in 10 Easy Lessons - 17 March 1979
* Who Needs a Green Eyed Jeannie? - 19 March 1979
RIPA buffer 和 鼠尾裂解液配方

**RIPA buffer**

1M Tris-HCL ph=7.6 (40X)

5Mm NaCL

10% Triton X-100 (10X)

10% dexoycholate sodium (脱氧胆酸钠)(10X)

20% SDS       (200x)

0.5M EDTA   (100x)

100mM PMSF  (100x)

100mM Na3VO4        (100x)

PI             (100x)

**50ml 1x RIPA**

1.25 ml  Tris

1.5 ml   NaCL

5ml     Trixton-100X

5ml     Sodium dexoycholate

250ul    SDS

500ul    EDTA

36.5ml    H20

蛋白酶抑制剂现用现加

**1X  final tail concertation of tail digestion buffer**

50mM  KCL

10Mm  Tris –HCL(PH 9.0)

0.1%Triton X-100

0.4 mg/ml proteinase K
Kenjou Tomi

Template:Infobox character
Template:Nihongo is a minor character who appears in *KiraKira☆Pretty Cure A La Mode*. She is Akira and Miku's grandmother. She lives nextdoor to Usami Ichika. She always lovingly watches over the Kenjou sisters.

## Appearance

Tomi is a short-statured old lady with a round face and white hair. She wears tan capri pants, a pink shirt and a short-sleeved mint vest. She also wears light orange socks and a red hairband. She is nearly always seen with a smile on her face. This smile includes her eyes, so they are rarely shown opened.

## Personality

A very gentle and kind old lady who cares very much for her granddaughters. Occasionally she helps out the neighborhood committee.

## History

Tomi has lived nextdoor to the Usamis for a long time. At the end of episode 5 Akira moves in with her, and in episode 6 she explains why she came to live with her grandmother.

Tomi herself isn't seen until episode 30, when she comes with Miku to the school festival at Akira's highschool. Together with the KiraPati girls they go around the school festival.

She appears again in episode 36, this time to cheer on Akira's Central Ichigozaka team at the inter-neighborhood sports day. In a flashback, she and Akira are seen helping out the neighborhood committee. When it turns out that Akira was participating in the activities while sick, the other team members take her place, and Tomi stays with Akira. Seeing the others do their best for her, Akira remembers how much everyone has done for her. She tells Tomi that she is really happy she came to live with her.

In episode 44, Tomi is seen bringing Akira a present from Ichika to cheer her up. Later that episode she calls for Akira who had gone to help Ichika study for her math test, to tell her Miku has disappeared from the hospital.

## Relationships

* Kenjou Akira - Her older granddaughter. She lives with Tomi because it's closer to her highschool.
* Kenjou Miku - Her younger granddaughter. She has been hospitalized for a long time and her parents moved to be closer to the hospital.

## Gallery

KKPCALM 30 Miku with her grandmother.pngMiku and TomiKKPCALM 30 Dormouse.pngDressed up for the school festivalKKPCALM 30 Ciel teaching Miku.pngWatching Ciel help Miku with decorating cookiesKKPCALM36-Tomi Akira neighborhood committee chairman.pngTomi and Akira visit the neighborhood committee chairmanKKPCALM36-Akira says she's glad she came to live with her grandma.pngAkira says she's glad she came to live with TomiKKPCALM36-Kenjou Tomi closeup eyes open.pngCloseup with eyes openKKPCALM36-Kenjou Tomi closeup smile.pngCloseup smilingKKPCALM44-Tomi brings Akira a present from Ichika.pngBringing Akira a present from IchikaKKPCALM44-Tomi tells Akira that Miku has disappeared.pngTelling Akira that Miku has disappeared from the hospitalKKPCALM45-Miku Tomi Akira at Xmas party.pngMiku, Tomi and Akira at the KiraPati Xmas party
Template:KKPCALM characters
学术讲座

## 2021年5月12日——一夜白头：Myth V Truth—云发育论坛2021年第四讲——哈佛大学 许雅捷

**Skin Deep: Stem Cells at the Nexus of the Niche, Physiology, and the External Environment**

**一夜白头：Myth versa Truth, Myth and Truth**

精神压力充满了现代社会生活的方方面面，与每一个人都有密切的关联,它广泛影响着身体各种组织器官和功能。压力如何影响组织生物学，成体干细胞和微环境，导致组织退化，一夜白头：Myth还是Truth, 哪些是Myth 哪些是 Truth……云发育论坛2021年第四讲，由哈佛大学皮肤干细胞学家许雅捷教授给大家带来毛发生长和衰老的秘密。

**Abstract**

Modern society is overrun by stress, which affects people of all ages, genders, and occupations. Stress is thought to influence a wide variety of tissues and processes in the body, and is perhaps the single most common risk factor for any disease or change in the skin. Despite its profound effect, surprisingly little is known about the mechanisms by which stress impacts tissue biology, and whether stress-related tissue degeneration is associated with the effect of stress on somatic stem cells, their niches, or their progeny. To understand whether and how stress affects tissue changes, my lab adapted several approaches to model both transient acute stress and long-lasting chronic stress and delineated their impacts on skin stem cells. Combining physiology, neurobiology, endocrinology, stem cell biology, and functional genetics, we have identified **two distinct mechanisms** by which stress affects different populations of stem cells in the skin. For melanocyte stem cells, stress-induced neuronal activity drives rapid loss of melanocyte stem cells directly, leading to premature hair graying. For hair follicle stem cells, elevated stress hormones from the adrenal glands under stress inhibits hair follicle regeneration and prolongs stem cell quiescence via the impact of stress hormones on the niche.
Qupath定量

QuPath软件进行IHC（免疫组化）染色的定量的最基础操作，每一步记得保存；撰写这篇指南的软件版本比较早（具体不记得了），你如果下载了最新版本，你可以自己对照着看一下，基本功能，大差不差。

## 1.Create a Project

所有定量工作进行前，要建立一个项目，该项目路径必须是一个空的文件夹 （所有命名用英文，不然最后导出的measurement放到excel可能会出现乱码）。

点击如图所示Create project按钮，选择新建好的空文件夹，点击确定即创建完毕，可在Image List下方看到你新建的项目名称（即为文件夹名称）（图1）。

## 2.Add Images

   点击create project按钮右侧的add images按钮导入图片，在出现的对话框中添加想要定量的属于该项目的图片，并在set image type下拉菜单中选择H-DAB图片类型，点击import按钮等进度条走完即完成图片的导入。

导入后，图片的明自会在项目里显示出来，双击图片名即可打开图片，在右侧窗口中显示图片（注意，PNG格式图片似乎不支持）（图2）。

## 3.Set Color Deconvolution

IHC染色图片即H-DAB类型图片在软件里分为三种颜色图层来识别，分别是苏木素的蓝色，DAB的棕褐色，背景的其他色，这一步大概意思是确定各个颜色的数值，让软件在接下来步骤中可以正确识别各个颜色。

在Tools菜单中选择任意喜欢的ROI工具，画出一个区域，这个区域要包括上述提到的三种颜色，即苏木素、DAB还有背景，然后点击analyze→preprocessing→estimate stain vectors，弹出的窗口中选择Auto，再点击确定即可完成这一步的设置。然后选中刚才的ROI删除即可（选中的方式有两种，第一是点击ROI边框，被选中的ROI边框会被高亮为黄色；另一种是在Annotations栏下进行选择）。

## 4.ROI

组织那么”大“一块，染色扫片出来，并不是所有区域的染色结果都符合你的”预期吧“；或许有些地方你“染得不好吧”；是不是有些地方有所谓的”边缘效应“呢。

一些时候你可能就像定量那些一下就能看出来趋势的地方，所以ROI工具就是你最好的帮手，选择喜欢的ROI，圈出来所有你想要定量的位置。有多种工具可以选择，方框，圈，画笔，魔术棒等。

需要注意的是，对于某些特殊定量要求，一些工具画的ROI和另一些工具画的ROI没法重叠，有一些可以操作我语言表述能力不强，有需要你可以去试试。

## 5.数细胞，Cell detection

Qupath软件定量是以细胞为单位的，每一个细胞返回一个数值，所以开始定量的第一步就是数细胞，我就以cell detection为例说明（如果是ki67数阳性率的要选择positive cell detection，操作类似不赘述）。

analyze→cell detection→cell detection，出现一个窗口，第一的下拉菜单中你会看到两个选择：1是按苏木素染色强度来数细胞（单一苏木素色彩通道），2是按总的光密度值来数细胞（所有颜色叠加），想用哪个就用哪个。

看有这么多参数，重点说两个：cell expansion，顾名思义是细胞的大小，决定了细胞质的面积，这个看你细胞的真实情况进行调整；threshold，细胞计数的最小强度值，如果像素块的AOD mean值小于它，软件就不会计数。

其他的呢你随便调吧，目的都是让细胞数得更准确（前文说过，图片带不带比例尺，数值的单位是不一样的，微米或者px，导致具体数值也不同）。

## 6.批处理，Run for Project

即把你对一张图片进行的操作应用到所有，这是重点，这也是我摒弃实验室传统方法，研究qupath定量软件的原因，这个真的省时间。

你对第一张图片进行完数细胞操作后，点击在左侧workflow那一栏，上面的list中记录你对该图片进行的所有操作，在下方点击create script，弹出来一个窗口，基本上会有set image type, set color deconvolution, select annotations, cell dectection，这些命令，点击该窗口上方run→run for project，在新窗口将想要进行批处理的图片选上，run开始。

## 7. 导出数据

在批处理结束之后，需要点击一下save，否则我们导出不了数据。点击measure→export measurement，然后选择我们要导出的路径并修改文件名，在export type下拉菜单中选择Cells（我们的数据是以细胞为单位的），点击populate，然后在column to include中选择我们想要导出的数据类型，导出即可。

1. ↑ https://doi.org/10.1038/s41598-017-17204-5
亚克隆引物设计及实验操作

亚克隆据百度百科说，是将一个基因从一个载体上转移到另一个载体上的克隆。我们一般采用同源重组的方式将目的基因插入到目的载体上。

设计引物：

一般来说根据目的基因两端序列，以及目的载体插入位点两端序列设计引物：同源部分在15-20bp，两端引物Tm值要小于10摄氏度。

注意事项：

对于pLVX载体，我们一般在正向引物加上EcoRI酶切位点，在反向引物加上BamHI酶切位点。

对于要加Tag的引物，如果加在目的基因5’端，我们在设计引物的时候，需要将Tag设计到起始密码子右侧；且要在Tag的两侧加上Kozak序列（GCCACC）和柔性蛋白序列（例如：Ggtggtggtggttca）；如果在3‘端则是中止密码子需要更改位置，其余同理。