recursal/Fanatic-Fandom huggingface dataset:
Danika (Gravity Hurts)

## Danika

**Miss Danika Pretty-Eyes** is Sage Valentino's imaginary friend from when Sage was 5 to 9 years old. She also guards the Memory Storage Room of Sage's Mindscape.

## Appearance

   Danika is a pale pink-tinted unicorn, who has a lighter pink aura around her. Her mane is a dull pink color and is fairly long. She has no tail. Her eyes are a vibrant blue color, and are usually closed. Her hooves are a pastel purple shade and has the same light colour tufts with hair right above the hooves.
Love Like Woe

**Love Like Woe** is the pairing of **Sagitarrius Pines** and **Robbie Valentino**.

 Overview

**Sage and Robbie** started out as enemies since the age of 9, but ended up as lovers thanks to Mabel matchmaking them.

## Gravity Hurts

### "Season One"

#### Little Summer Boy

Robbie and Sage are revealed to be dating and the parents of Hudson and Dixie. They are seen both sitting on the couch watching TV as Hudson is trying to sneak Eileen inside the kitchen. Sage notices her son and Eileen and quickly calls out to him in confusion, which freaks Hudson out. Sage walks into the kitchen and asks why Eileen was just smuggled into the kitchen as if she was some kind of secret item, then notices the two reading the 4th journal.

#### RV There Yet?

the two are first seen in the garage of their house. Dixie walks in and asks the two if she could borrow the car, in which Robbie declines her request and tells her how she is not allowed to drive for the rest of the summer break, because of her accident in Crabital C.

## Alternate Universes

### Reverse Falls AU

An AU where characters like Dipper and Mabel switch places with characters like Pacifica and Gideon.

### Lady And The Tramp AU

An AU where Sage is a refined lady who lives in an upperclass family and Robbie is a mutt, and the two meet and go on many romantic adventures and fall in love.

#### Reverse Lady And The Tramp AU

An AU where Robbie takes the place of Lady and Sage takes the place of Tramp.

### Monster Falls AU

An AU where everyone is a monster. Sage is a faun and Robbie is a zombie.

### Childhood Friends AU

An AU where Sage and Robbie are childhood friends instead of fitting with the enemies to lovers trope.

### Mystery Buddies AU

An AU where Robbie and Sage are the two solving the mysteries of Gravity Falls.

### Mystery Inc AU

An AU where characters are representing characters from Scooby Doo and solve mysteries involving supernatural creatures and a lot of missteps.

### Partners In Crime AU

An AU where Sage and Robbie Valentino solve mysteries and search for enemy spies in 1950s Gravity Falls.
Beboerne på gården

Sjaske og Flopper er et par revolutionerende som Nancy og Albert møder uden for skoven. De bor på en gård med en ukendt antal af deres egne børn og sørger fuldstændigt for sig selv. Selvom de er i et højt antal, er der ingen af børnene der sulter, tværtimod er alle sunde og raske. Hver dag kører Sjaske, moren, ud og roder rundt i de omkringliggende huse efter mad og drikker. Dette nabolag kalder de “et skattekammer af en endnu ukendt størrelse”. Deres gårdsplads er belagt med brosten med byggemateriale spredt over det hele på gården. Flopper er en høj, tynd mand og er faren til deres børn. Har går med dreadlocks og sørger hovedsageligt for at passe på børnene og lege med dem. Selvom han kaldes Flopper, hedder han faktisk Rikki. Alle deres børn har en enorm mængde af energi, der skaber en stemning som smittes på Nancy og Albert da de først ankommer til gården. Børnene tilbringer størstedelen af deres tid til at øve sig på en hel række af forskellige ting. Nogle øver sig på at stå på hænder, hopper over hinandens skuldre, og ellers også en del andre atletiske aktiviteter. Nogle af de ældre børn laver flikflak, en af de store drenge jonglere med fire bolde, mens han holder en fodbold i luften med et af sine ben. De tre mindste børn balancerer med kegler og hulahopringe på næsen, mens en af pigerne går på linje over gårdspladsen fra tag til tag. De leger ikke, de træner og øver sig hårdt. GÅrden er næsten som et cirkus. En anden pige spyer ild og en dreng sætter ild til ham selv og slukker det med det samme igen. De vil lave deres egen hær af deres egne børn. De har en baby ved navn “Anarkia”.

Gården blev købt af nogle hippier, men alt andet har de selv skulle konstruere og sørge for. Eksempelvis har Flopper skulle reparere deres tag, men uden en uddannelse i lige dette, er arbejdet gjort sjusket men det virker. De var oprindeligt fra københavn, men flyttede til øen efter bz-bevægelsen.
Agano Shimizu

**Agano Shimizu** (あがの しみず *Shimizu ag a no*), also known as **Tender**(やりこい *Yarikoi*), is a student in Class 1-C at U.A. High School, training to become a Pro Hero.

 Appearance

## Abilities

**Overall Abilities**:

**Tactical Intellect**:

## Battles & Events

**Entrance Exam Arc**

* U.A. Entrance Exam

**Quirk Apprehension Test Arc**

* Quirk Apprehension Test

**Battle Trial Arc**
**U.S.J. Arc**

* U.S.J. Incident
	+ U.A. Students vs. Kurogiri

**U.A. Sports Festival Arc**

* U.A. Sports Festival
	+ Obstacle Race
	+ Cavalry Battle

**Vs. Hero Killer Arc**

* Hero Agency Internships
* Hosu Incident
	+ U.A. Students vs. Hero Killer: Stain
* Rescue Training Race

**Final Exams Arc**

* First Term Final Exam
	+ Team Iida and Ojiro vs. Power Loader

**My Hero Academia: Two Heroes**

* I-Island Incident
	+ U.A. Students vs. I-Island Security Bots
	+ All Might & Deku vs. Wolfram

**Forest Training Camp Arc**

* Quirk Training Camp
* Vanguard Action Squad Invasion

**Provisional Hero License Exam Arc**

* Provisional Hero License Exam
	+ Crushing of U.A.

**Shie Hassaikai Arc**
**U.A. School Festival Arc**

* U.A. School Festival

**Joint Training Arc**

* Joint Training Battle
	+ Round 3

**Endeavor Agency Arc**

* Hero Interview Training
* Hero Work-Studies II
Benny Weir

**Benny Weir** is Ethan's loud and goofy best friend, who is born with the power to cast spells and use magic. He often gets into trouble with his antics. He is a student at Whitechapel High.

## Background

Benny has been Ethan's louder, goofier best friend since they were both six years old. While he is just as brave and strategically clever as his pal, Benny admits that in his case it only applies to Video games. Benny's dream is to one day make it big as a sorcerer or wizard, paranormal expert, and babe magnet. He discovers that he is a spellmaster right before the Dusk III: Unbitten advance screening when his Grandma Weir gives him a spellbook and Ethan some daggers. He is similar to Ethan, being geeky and book-smart. He and Ethan understand each other better than anyone else, although Benny is more funny and outspoken than Ethan while Ethan is the more serious and sarcastic one. Benny often tries to lighten the mood when things get too intense. He is girl-crazy and frequently drags Ethan into his schemes, getting them both in trouble. Despite that, Benny is a genuine friend to Ethan and is always ready to go on another adventure. He is similar to Ethan, book-smart with an encyclopedic knowledge of science fiction, comic books and video games. Benny often struggles with how to most effectively use magic but practices daily to improve. In season 2, it's his second year in Whitechapel High and is still continuing his skills as a Spellmaster while surviving typical High School challanges.

## Abilities

As a Spellmaster, Benny can cast spells, and make potions like a wizard. His skills, however, work mostly incorrectly and miss, as a large number of his attempts don't work the way he intends, for instance he once accidentally turned Ethan into a werewolf (*Blue Moon*). His skills with potion making is shown to be better, as they normally work. However he is generally best with simple magic.  He also possibly possesses the ability to sense other spells, for instance he could sense that the cheerleading chant was actually a spell in *Three Cheers for Evil*.

However in season 2, following a lot of practice he gets better at magic, mastering more complex spells such as erasing memories, knocking others unconscious, and reversal spells.

## Trivia:

* His favorite type of coffee is a half-caff low fat latte mocha chino no foam and a half-caff low fat latte mocha chino. (*The Brewed/The Movie*)
* He is also a fan of the Star Wars franchise, as shown in the film when he pauses to acknowledge the vendor's vintage Star Wars shirt during combat with vampires. (The Movie)
* He used to be afraid of robots when he was six. (*The Movie*)
* He had/has a crush on Catwoman. (*Die Pod)*
* He was/is afraid of Cyberdontist. (*Independence Daze*)
Abbie (Total Drama Student Ambitions)

**Abbie** was a contestant on Total Drama Student Ambitions as a member of the Consumers.

## Personality

Abbie is extremely positive and cheerful, which often keeps her going during challanges.

## Total Drama Student Ambitions

Abbie was first introduced in School Orientation along with her

## Trivia

### General

* Abbie was entirely based on the 'Girl Scout' song from the Beetlejuice musical.
Cité des Dieux

Le Royaume Hivernal contient des Pierres d'Illumination, objet nécessaire à l'Exaltation des Héros de Lumière. Ce Donjon est ouvert les lundis, mercredis, vendredis et dimanches.

Baal possède une Défense incroyablement élevée. À moins de posséder un Héros capable d'ignorer la Défense adverse, il faudra d'abord se débarrasser de ses sbires afin de rendre le combat un peu moins difficile. Attaquez d'abord l'Élémentaire de Feu et vous pourrez respirer un peu car il inflige de lourds dégâts. Vous pourrez ensuite soit abattre l'Élémentaire d'Eau en prévision d'un long combat pour ne plus subir de perte de Défense, ou bien attaquer directement Baal, surtout si encore une fois vous possédez un Héros capable d'ignorer la Défense adverse.
Vaughn Lowey

**Vaughn** was a contestant on Total Drama Project Reality as a member of The Phantom Vikings.

## Personality

Just like his label says, Vaughn is lacking of empathy and quite the motormouth, making it hard for people to understand him. Vaughn usually is quite snarky, but often lets Lena be the more snarky one out of the two. Due to the lack of empathy, Vaughn can sometimes be a bit innocently insensitive.

## Total DramaRama

*View this page for his Total DramaRama counterpart.*

## Trivia

### References

* Vaughn is inspired by the Beetlejuice character, Betelgeuse.
Lana Pines

**Lana Pines** is a mermaid human hybrid and the daughter of Mermando & Mabel Pines. She is the cousin of Hudson and Dixie.

## Personality

Lana has shown to be really easy-going and carefree. She has proven herself to also be extremely dependable and able to hold secrets by keeping Eileen's secret about her liking Hudson.
Template Guidance

This is the guide for how to use **Templates** correctly, or well so!!

## Fandom Template

Templates for specific fandoms are always welcomed to be made or requested! Underneath this will be showcases of fandom-related templates, with text explaining what should go in what row!

## Background information

### Last episode

What episode they appeared in last. If they have only had one appearance, this should be blank.

### Voice

If this character has spoken, a voice actor should be listed here.

### Inspiration

If this character is inspired by someone or something, it should be listed here.

## Character information

### Other names

If the character has any nicknames, aliases or such they should be listed here.

### Age

How old is the character? If a birthyear is revealed, it should be put here also.

### Personality

A short summary of their personality should be listed here.

### Appearance

A short description of what the character looks like should be here.

### Occupation

If the character is a student or has a job, it should be listed here.

### Alignment

If the character is Evil, Good or neutral, it should be listed here.

### Goal

If the character has a goal or two(or more), it should be listed here.

### Affiliations

If the character is affiliated with something, like Mabel is affiliated to the Mystery Shack or .GIFfany is to Love Academy 7, it should be listed here.

### Home

If where the character lives is confirmed, it should be listed here. If the city and not a specific location is confirmed, it should be listed instead of a fanon location.

### Relatives

If the character has any relatives, they should be listed here.

### Pets

If the character has any pets, they should be listed here.

### Allies

If the character has any allies, they should be listed here.

### Enemies

If the character has any enemies, they should be listed here.
Sage Bothman

**Sage Bothman** is the *Be More Chill* self-insert of Sage.

## Alternative Universes

### Squip AU

Haruka Sagah is a squip that modded by a famous modder from New Jersey called 6uru that happens to be the squip sold to Jeremy Heere. Instead of wanting to dominate the world, this squip will try to help Jeremy but ends up accidentally getting feelings and falling for the awkward teen boy.

The modding of this squip gives them updates most other squips don't have.

### Reverse Squip AU

It is basically the same as the Squip AU, but Sagah ends up wanting to dominate the world.
Jeremy Heere

**Jeremy Heere** is the main protagonist of Be More Chill, and a junior at Middleborough High School. He is uncool, uninteresting, and wishes he can do more than survive in high school, as well as get his long time crush Christine Canigula to notice him. After learning about the Squip from his bully, Rich Goranski, Jeremy buys one and takes it, and learns how to become popular with help of his Squip, not real the dangers that await him.

## Character Description

Jeremy is sweet, introspective, awkward, and very much uncool, and he just hopes to keep his head down enough to survive high school and maybe get the girl of his dreams, Christine Canigula, to notice him. When he's given the opportunity to have a supercomputer called a Squip (Super Quantum Unit Intel Processor) implanted in his brain that tells him how to become popular, he has to balance following its rules with following his own heart.

## Physical appearance

Jeremy is often called "Tall-ass" by Rich. When talking to him in the bathroom, he mentions that he's "not even that tall", and Rich says that he "would be if [he wasn't] slouched over all the time." After he gets his Squip, he stops slouching.

During the beginning of the musical, he wears a striped yellow and blue shirt with a dark blue cardigan as well as glasses in the Broadway production but eventually changes into an Eminem shirt after he gets his Squip. He also stops needing his glasses when he has the Squip, due to it fixing his vision.

## Trivia

* His best friend is Michael, and they are considered Player 1 and Player 2, with Jeremy being Player 2.
* He signs up for the school play for an opportunity to get closer to Christine.
* He's a Junior in High school.
* His parents are divorced, and he lives with his dad (who has a habit of not wearing pants, which annoys Jeremy).
* He's Jewish.
* The Squip gives him an Eminem shirt to wear, which he does, not knowing that Eminem died. As a result, Brooke takes pity on him and they grow closer in "Upgrade".
* He becomes friends with his former bully Rich Goranski while under influence of the Squip.
* His original Squip looks and sounds like Keanu Reeves
Tut gordoburgo

tut gordoburgo es un cerdo de 30 años malhumorado y engreido,odia a robert,zack y ryan lo que lo llevo a secuestrar a eric goldberg,tut gordoburgo tiene un hijo llamado pup gordoburgo,quien a diferencia de tut es amigo de robert,tut no esta de acuerdo con esto y siempre trata de alejar a pup de esos ''maricas'' forma en la que tut los llama,es alcoholico y tambien es un padre incompetente,su frase caracteristica es ''salgan de mi jardin idiotas''
Sage's Self-ships

This is a list of **Sage's Self-ships**. This list is listed alphabetically. Bolded out characters are current main self-ships.

## Romantic F/Os

### A

Adam Maitland

Alistair Theirin

Anita Radcliffe

Asgore Dreemurr

### B

Barbara Maitland

Barry (Pokémon)

Bif Taylor

Bran Stark

Brock (Pokémon)

### C

Cameron Frye

Cody (Total Drama)

### E

Eddie Kaspbrak

Erik The Slayer

### F

Ferris Bueller

Fluttershy +

### G

Gary Smith

GlaDOS

### H

Haida

Hanta Sero

Hop (Pokémon)

### I

Indigo Wreath + Enótita = Approach With Care

### J

Jeremy Heere + Sage Bothman =

Jimmy Hopkins + Sage Holland Drage = Two Bisexual Catastrophes

Jonah Simms + Sage Halley Kitty Mitnick =

Jude Lizowski + Sage Attberry = Sk8er Boi

### K

Katsuki Bakugo

Kazuichi Soda + Chouko Yoshida =

Kili

**Knuckles the Echidna + Sage the Cat = Set Your Heart Free**

### M

Mark Jarvis

Max Goof

Michael Kelso

Miles Morales

Moira Burton

Monika

Mr. Mistoffelees

### P

Peregrin Took

Pete Kowalski

Peter Parker

Piers Nivans

### R

Rarity

**Robbie Valentino + Sagittarius Pines = Love Like Woe**

Roger Radcliffe

### S

Seth Hadaway Alden

Seth Haber Ackerman

Shane +

Sherry Birkin

Shoto Todoroki

Sonic the Hedgehog

Soul Evans

Steve (Minecraft) + Sage (Minecraft)

### T

Tenya Iida + Chouko Serizawa =

The Scout

Tom Gurney

Tommy Jarvis

## Platonic F/Os

### C

Clint (Stardew Valley)

Cream the Rabbit

### R

Rhys Strongfork

Robin (Stardew Valley)

## Familial F/Os

### C

Cassie Lang (Sister)

Chester Davies (Harry Potter) (Brother)

### L

Laura Kinney (Sister)

Logan Howlett (Father)

Lydia Deetz (Adoptive daughter)

### M

Marnie (Stardew Valley) (aunt-in law)

**Mabel Pines (sister)**

### S

Scott Lang (god-father)

**Stanford Pines (Grand-uncle)**

**Stanley Pines (Grand-uncle)**

### T

Thor Odinson (Brother)

Tony Stark (God-father)
Gravity Falls (disambiguation)

**Gravity Falls** may refer to:

## Series generalization

1. Gravity Falls (TV series), the Disney Channel Original Series.
2. "Gravity Falls Main Title Theme," the theme song of the series.
3. Gravity Falls: International Versions, a guide to *Gravity Falls* in other countries.
4. Gravity Falls: Broadcast List, a guide to the worldwide broadcasting of *Gravity Falls*.
5. Gravity Falls special events, list of marathons and events concerning the series.

## Locations

1. Gravity Falls, Oregon, the location where the show takes place.
2. Gravity Falls Forest, an area surrounding most of the town.
3. Gravity Falls Gossiper, a newspaper publisher and building.
4. Gravity Falls Civic Center and Buffet, the public building.
5. Gravity Falls Junkyard, the town's local junkyard.
6. Gravity Malls, a local shopping mall.
7. Gravity Falls Museum of History, a museum detailing the history of the town.
8. Gravity Falls Pool, a public pool in the town.
9. Gravity Falls Water Tower, the water tower of the town.
10. Gravity Falls Cemetery, a cemetery located in the town.
11. Gravity Falls Maximum Security Prison, the state penitentiary.
12. Gravity Falls Swap Meet, a flea market.
13. Gravity Falls High School, a local school.
14. Gravity Falls Library, a public library located in the town.
15. Gravity Falls Police Department, the law enforcement team.

## Merchandise

1. *Gravity Falls: Legend of the Gnome Gemulets*, the *Gravity Falls* Nintendo 3DS game.
2. *Gravity Falls: Six Strange Tales*, the first DVD vol. release of season 1.
3. *Gravity Falls: Even Stranger*, the second DVD vol. release of season 1.
4. Gravity Falls apparel, a series of officially licensed clothing.
5. Gravity Falls Vinylmation, variation of a brand of Vinylmations.
6. *Gravity Falls: Journal 3*, the real-life version of *Journal 3*.
7. Gravity Falls Funko Pop! Vinyls Figures

## Others

Gravity Falls Public Access TV, the television network.
Categorization of pages

This is the guide for how to categorize pages to the right categories!! This page will also include a small guide for all the categories!

## Original Characters

Original characters are often categorized as "Original Characters"(Obvious, I know), "Characters" (all character pages are to be categorized as this) and if a character is a self-insert, please do categorize them as "Self-Inserts".

## Canon Characters

A canon character, also known as an official character from a piece of media, is often categorized as "Canon Characters" and "Characters".
Seth Haber Ackerman

**Seth Haber Ackerman** is a contestant on Total Drama Teen Tension and a member of the Curved Chickens.

## Personality

Seth is considered one of the smartest of the contestants and the most snarky one. Always with a comeback or an answer for a question, Seth has it all, including the looks, according to his admirer. He may not be the most social or open, but he can still sometimes be read like an open book.
Gisement

Les gisements sont des territoires à conquérir pour y gagner de l'or et des gemmes. Le temps joue sur le nombre de gains que le territoire peut vous donner au maximum, néanmoins attention aux pillages et exploitation ennemies pouvant vous faire perdre votre productivité.

**Petit conseils** :   - La Puissance des monstres gardant les mines sont assez élevé mais très facilement gagnable  même avec 30% de moins que eux en termes de puissance globale :)

                        - prendre le 2ème emplacement d'exploitation peut être judicieux mais seulement après avoir    atteint les 180-200kk de puissance global.
Radion (Gravity Hurts)

## Radion

**Radion** is a god who possesses the appearance of a bat-coyote-eagle mix who is often associated with desires, the wanting to be sanctuarized and the want to fulfill one's life goal. He was once a normal human, but got turned into a Kouza.

## Background

Ralph used to be a quite normal person and he used to own the sawmill in Gravity Falls. He showed much desire to be
Kitty Cox

**Kitty Cox** is a playable character of the Duck Duck Goose DLC of *The Night Ripper*.

## Appearance

Kitty Cox is a female caucasian young adult in her 20's. Her hair is of medium length and a dark brown color.

## Role

Kitty Cox is a young investigator who records her investigations and works for the local group of citizens who owns a radion broadcasting show where they uncover both old and recent cases, from all over the world. One foggy night, she is leaving work late to investigate the streets where one of the most recent victims was killed but is soon pursued by the duck-faced killer herself.
List of Total Drama Broadway Dream Roles

The following is a **list of Total Drama Broadway Dream roles**, listed alphabetically.

## Allen

### Narrator

The **Narrator** is the one describing the story of the famous actor Clay Dickson and how he relentlessly stalked his unnamed victim.

## Arie

### Addi

**Addi** is the secretary at the company Friday Banks, Norman and Cordie work at. She shows romantic interest in Cordie and eventually ends up with him in the end.

## Bodie

### Norman

**Norman** is the boss of Friday Banks and her love interest. On several occasions, he has acted upon finding Friday attractive and has made several sexual comments about her, making her extremely uncomfortable. Although making sexual comments that make Friday feel uncomfortable, Norman is shown to worry about her and often shows to care about how she's doing, mostly after her breakdown.

## Henley

### Friday Banks

**Friday Banks** is the main character of *Lawsuit for Office Romance*. She was portrayed as a hard-working woman who is often considered a bit too pretty for her workplace. During the first act, she is portrayed as feeling unaccomplished and during most scenes in the first act wishing for a change in her life. She shows to hate her boss Norman during the start, until her breakdown where he's one of the few people who show to care about her. Towards the end, she realizes she loves Norman and her hometown, which she had planned to leave at some point but never did.

## Jordan

### Cordie

Cordie is the intern of Norman and an admirer of Friday Banks, who he realizes does not reincorporate his feelings and soon realizes Addi has always been there for him.
Self-Inserting Heaven Wiki

**Welcome to the Self-Inserting Heaven Wiki!**
We are a cooperative community site for any self-inserter or self-shipper to expand upon! The wiki rely on readers and editions who get involved and add content, all about canon and original content!

We welcome most type of content that does not harm anyone! So anything from original characters, articles about your Fictional Others, fanfictions, F/O lists, and articles about songs. Just remember to credit where credit is due, like for example on pages about canon content like characters or locations, or even concepts!

## Important articles

PlaceholderA page about your topicPlaceholderA Main CharacterPlaceholderThe First EpisodePlaceholderAn Important LocationPlaceholderA Key EventPlaceholderA Crucial Item

*Need help building out this community?*

* Page categorization
* Title naming
* About disambiguations
Villagers (Stardew Valley)

¨

**Villagers** are characters in *Stardew Valley*. They are citizens who live in and around Pelican Town. Each villager has a daily routine, so they can be located in different sections of town depending on the in game time of the day.

Villagers provide quests and can be given gifts to increase affection. Each villager has unique likes and dislikes, and will respond to gifts differently.

*For villager gift preferences, see the following pages:*
*Friendship*
*List of All Gifts*

## Marriage Candidates

### Bachelors

* [1]

Alex

* [2]

Elliott

* [3]

Harvey

* [4]

Sam

* [5]

Sebastian

* [6]

Shane

### Bachelorettes

* [7]

Abigail

* [8]

Emily

* [9]

Haley

* [10]

Leah

* [11]

Maru

* [12]

Penny

## Non-marriage candidates

* [13]

Caroline

* [14]

Clint

* [15]

Demetrius

* [16]

Dwarf

* [17]

Evelyn

* [18]

George

* [19]

Gus

* [20]

Jas

* [21]

Jodi

* [22]

Kent

* [23]

Krobus

* [24]

Lewis

* [25]

Linus

* [26]

Marnie

* [27]

Pam

* [28]

Pierre

* [29]

Robin

* [30]

Sandy

* [31]

Vincent

* [32]

Willy

* [33]

Wizard

## Non-giftable NPCs

* [34]

Bouncer

* [35]

Gil

* [36]

Governor

* [37]

Grandpa

* [38]

Gunther

* [39]

Henchman

* [40]

Marlon

* [41]

Morris

* [42]

Mr. Qi
Baldur's Gate

Heapside

Baldur's Gate est une ville côtière située au bord de la Sea of Swords. C'est un carrefour commercial important du fait de son accès à l'intérieur du continent de Faerûn par la rivière Chionthar.

## Organisation

Baldur's Gate est dirigé par un conseil de 4 ducs dont un grand duc. Le grand duc actuel de la ville est Ulder Ravenguard. L'ordre est maintenu par la milice des Flaming Fist dont Baldur's Gate est la base opérationnelle.

## Événements

D'étranges événements se sont produits récemment dans la région, le plus marquant étant la disparition soudaine du grand duc Ulder Ravenguard. Une guerre de pouvoir s'est enclenchée entre les deux principaux candidats au titre, les ducs Darmin Zodge et Dillard Portyr.

La cité voisine de Elturel ayant également disparu soudainement et sans explications, de nombreux migrants et réfugiés sont arrivés à Baldur's Gate. Les autorités ont donc rapidement fermé les portes et la ville s'est refermée sur elle-même.
Arthas Jr.

Arthas Jr. est une liche qui détient la taverne Deadman's Inn à Baldur's Gate.

## Histoire

Aucun élément sur le passé d'Arthas Jr. n'a été révélé.

## Lien avec les Hellsong Pierce Eyes

Il rencontre les Hellsong Pierce Eyes lorsqu'ils entrent dans son bar à la recherche d'informations sur le culte des 3 Mortifères. Il accepte de les aider en échange d'une affaire avec Bebero Rougenez, qui lui offre de la bière empoisonnée au venin de son serpent Master Snakou contre des informations. Arthas Jr. leur indique donc d'aller consulter Dujek One eye.
3 Mortifères

Culte des 3 mortifères vénérant les dieux suivants:

Le dieu Bahal: dieu du chaos. Ses fidèles ont la réputation de pratiquer l'assassinat dans la forme la pure qu'il soit.

Le dieu Bane: dieu de la tyrannie.

Le dieu Mirkul: dieu des os et de la nécromancie. Ses adeptes sont entrainés à des rites qui permettent aux mortels de communiquer avec des personnes décédées.
Master Snakou

Master Snakou est un Anaconda venimeux de 8 mètres de long venant des Serpent's Hills.

## Histoire

Master Snakou vient des Serpent's Hills, un marais situé au nord de Baldur's Gate. Il fut capturé par un groupe de braconniers après avoir tenté d'en faire son déjeuner. Il est ensuite amené sur les marchés de Baldur's Gate où il sera acheté par le propriétaire du Pet Store.

## Lien avec les Hellsong Pierce Eyes

Lors d'un passage au Pet Store, Bebero Rougenez l'adopta pour 30 pièces d'or. Il est depuis son animal de compagnie et coopère principalement pour la création de boissons "épicées" à base de venin de serpent et pour attaquer furtivement des ennemis.
Deadman's Inn

Le Deadman's Inn est une taverne située à Baldur's Gate et gérée par Arthas Jr.. Cette taverne est réputée pour être un lieu de magouilles où les malfrats de la ville se retrouvent pour faire affaire.

## Evénements

Les Hellsong Pierce Eyes sont passés dans cette taverne afin de trouver des informations sur le culte des 3 Mortifères. Ils ont pu obtenir des indications de la part d'Arthas Jr. en échange d'une bière relevée au poison de serpent fabriquée pas Bebero.

Ereël et Akreon ont également remarqué un accès à des sous-terrains dans la cave de la taverne. Il est possible que ces sous-terrains mennent à l'Under Dark.
Sage the Cat

**Sage the Cat** (サルビア ザ キャット *Sarubia za Kyatto*) is a fictional character from the *Sonic the Hedgehog* series. She is an anthropomorphic cat with various enchantment and skill-related abilities. She is the daughter of King Dorian and Queen Lucille of the Nereth dynasty. She wields the [sword (Sonic The Hedgehog)|Purifier sword], a large mithril blade, which she was given by her father.
Scoring of Apoptotic Nuclei

* Hermaphrodites were picked as late-stage L4s and allowed to age for 22-26 hours.
* They were mounted in M9 on 1.5% agarose pads containing 0.2 mM levamisole and their gonads examined by fluorescence microscopy for cells completely surrounded by ced1::GFP. 30-40 gonads are scored per genotype
* make agar pads that contain levamisole (the stock solution is 1000X; this immobilizes the worms) fresh every-time; for dumpy worms this stock is 2000X
* keep the agar at ~95 degrees while making each pad
* flank the slide that you are making your pad on with a slide and coverslip on either side
* put a drop of 1.5% agarose + levamisole on a slide and drop a 22X50 coverslip perpendicular to the slide on top to flatten.
* leave for 3 minutes
* slide the coverslip off and add drop of oil
* place worms on the pad quickly so it doesn’t dry out
* put 22X22 coverslip on top
* seal with agarose
* add 10-25 ul M9 underneath coverslip
* visualize on Deltavision with 100X objective
Sagitarrius Valentino (Gravity Hurts)

## Sagitarrius Pines

### Personality

Cheerful, moody, sisterly, awkward, good-hearted, selfless, romantic, anxious, friendly, maternal, accepting,

### Relatives

Mr. and Mrs. Pines(parents)Mabel Pines(younger sister)
Dipper Pines(younger brother)
Shermie Pines(grandfather)
Filbrick Pines(great-grandfather)
Caryn Pines(great-grandmother)
Stan Pines(great-granduncle)
Marilyn Pines(great-grandaunt)
Ford Pines(great-granduncle)
Robbie Valentino(husband)
Hudson Valentino(son)
Dixie Valentino(daughter)

### Powers and abilities

Art, designing, caretaking, singing, knitting, baking, sewing

**Sagitarrius "Sage" Pines Valentino** is a major character in Gravity Hurts and the father of Hudson and Dixie Valentino, and the wife of Robbie Valentino.
Oreo Shake

Hello, This Page is written by the one and only, Oreo Shake

I competed in a camp called Camp Lazy, I was the quiet type and never talked to anyone, I sucked at challenges and never won one, my alliance members were Cool 90's Theme and Green, we had a plan to eliminate others but sadly it was a tie between my alliance members once and Green had to go, then me and Handicapped Parking Sign tied in the next elimination, but that is when I was eliminated, 9th place out of 20, although I was sad I was leaving the game, the game experience was fun for me, I'm just glad C9T won my friend, although Camp Lazy is no longer existing (Well I don't know), I had a great time and looking foward to compete again in another camp.
10B Competent Cell Preparation

*Adopted from Stone Lab protocol by Christian (May 2016)*

**Strain** = NB570, 10-Beta Cells (Streptomycin resistant)

## **Solutions**

**LB:**

* 1% bactotryptone
* 0.5% yeast extract
* 1% NaCl. Autoclave 40 min.

**SOB (Maniatis):**

* 2% bactotryptone
* 0.5% yeast extract
* 0.05% NaCl
* 2.5mM KCl. pH to 7 with NaOH
* Autoclave 40 min, then add MgCl2 and MgSO4 to 10mM before using

**RF1:**

* 100mM RbCl
* 50mM MnCl2-H2O
* 30mM KOAc
* 10mM CaCl2-2H2O
* 15% glycerol
* pH to 5.8 with acetic acid (CH3COOH) and sterile filter.

**RF2:**

* 10mM MOPS
* 10mM RbCl
* 75mM CaCl2-2H2O
* 15% glycerol
* pH to 6.8 with NaOH and sterile filter.

## **Protocol**

*Notes:*

* Always keep cells on ice.
* Use Avanti J-26 XP centrifuge (Sin 112) at 4°C with JA-20 rotor.
* I usually double this protocol to avoid making cells constantly.

1. Grow 5mL culture overnight in LB (from a single colony).

2. Add 2mL overnight culture in 100mL SOB + 10mM MgCl2 + 10mM MgSO4. *Note*: I’ve substituted SOC or 2XTY supplemented with MgCl2/MgSO4 instead of the SOB without any issues.

3. Grow ~2 hours @ 37C.

4. Check OD. When OD is between 0.4-0.6, cells are ready to harvest.

5. Split into 2x 50mL tubes. a. Note: use JA-20 rotor tubes.

6. Ice cells 10 minutes.

7. Spin 3K for 10 minutes @ 4C. Remove sup.

8. Resuspend each pellet with 16mL ice cold RF1 (16mL per 50mL tube). *Note*: Can vortex gently or use pipetteman. Be gentle.

9. Ice cells 15 minutes.

10. Spin 3K for 10 minutes @ 4C. Remove sup.

11. Resuspend each pellet with 4mL ice cold RF2 (4mL per 50mL tube).

12. Ice cells 15 minutes.

13. Make 100uL aliquots on ice (or dry ice) in 500uL microcentrifuge tubes. a. Note: I use metal racks to chill lots of tubes at once.

14. Flash freeze aliquots in liquid N2.

15. Store @ -80C. 16. Test competency. Transforming 100ng of plasmid should give lawn.
Antibiotic Stock Solutions

## 50 mg/ml Carbenicillin (1000X)

1. Add 500 mg Carbenicillin to 10 mls distolled water in 15 ml falcon tube
2. Mix to dissolve
3. Filter through syringe filter
4. Aliquot 550 μl per 1.5 ml Eppendorf tube
5. Store at -20 °C

## 50 mg/ml Kanamycin (1000X)

1. Add 500 mg Kanamycin to 100 mls distilled water in 15 ml falcon tube
2. Mix to dissolve
3. Filter through syringe filter
4. Aliquot 550 μl per 1.5 ml Eppendorf tube
5. Store at -20 °C

## 25 mg/ml Chloramphenicol (1000X)

1. Add 250 mg Chloramphenicol to 10 ms EtOH in 15 ml falcon tube
2. Mix to dissolve
3. Aliquot 550 μl per 1.5 ml Eppendorf tube
4. Store at -20 °C

## 50 mg/ml Streptomycin (1000X)

1. Add 500 mg Streptomycin to 10 ml distilled water in 15 ml falcon tube
2. Mix to dissolve
3. Filter through syringe filter
4. Aliquot 550 μl per 1.5 ml Eppendorf tube
5. Store at -20 °C
S Basal

1. To 400 ml distilled H2O add:
	* 5.85 g NaCl
	* 1 g K2HPO4
	* 6 g KH2PO4
	* 1 ml cholesterol (5 mg/ml in ethanol)
2. Bring final volume to 1 L and re-mix
3. Label and divide into 10 100 ml aliquots
4. Autoclave for sterilization

## For 2L S Basal

1. To 800 ml distilled H2O add:
	* 11.7 g NaCl
	* 2 g K2HPO4
	* 12 g KH2PO4
	* 2 ml cholesterol (5 mg/ml in ethanol)
2. Bring final volume to 1 L and re-mix
3. Label and divide into 10 100 ml aliquots
4. Autoclave for sterilization

## Making Complete S Basal (S Medium)

1. Follow steps for S Basal as directed above
2. Let cool and to each bottle add aseptically:
	1. 1 ml 1M Potassium Citrate, pH 6.0
	2. 1 ml Trace Metals solution
	3. 300 μl 1M MgSO4
	4. 300 μl 1M CaCl2
Co-CRISPR RNP Injection Mix Protocol

1. Resuspend *target*-gRNA and *dpy*-10-gRNA in IDT duplex buffer
	* Add 10ul duplex buffer to each to resuspend to 200uM gRNA
		+ *Keep resuspended RNAs on ice to prevent degradation*
2. Resuspend dsDNA Ultramer oligo repair templates in water
	* Add 80ul RNAse-free water to resuspend to 50uM dsDNA oligo
		+ *\*If using plasmid repair template, be sure to phenol:chloroform purify it to remove RNAses before adding it to your mix*
3. Duplex 200uM *target*-gRNA and 200uM *dpy*-10-gRNA with 200uM tracrRNA
	* Add 2.25ul target gRNA, 0.25ul dpy-10 gRNA, and 2.5ul tracrRNA in RNAse-free microfuge tube
	* Incubate at 95°C for 5 minutes
	* Spin down briefly
	* Incubate at RT for 5 minutes
		+ *\*\* The Zahler lab uses a 1:9 dpy-10-gRNA to target-gRNA ratio, and the Dernburg lab uses a 1:12 dpy-10-gRNA to target-gRNA ratio.*
4. Complex *target*-gRNA:*dpy*-10-gRNA:tracrRNA duplex with Cas9 protein
	* Add 5ul 40uM Cas9 protein (freshly thawed from -80°C) to 5ul duplexed RNA
	* Incubate at RT for 5 minutes
5. Add target repair template
	* If using ssDNA Ultramer oligo repair template, add 1.3ul 50uM ssDNA target repair oligo, for ~6uM final concentration
	* If using plasmid repair template, add (phenol:chloroform purified) plasmid to final concentration of 10-30ng/ul
6. Add *dyp*-10 dsDNA Ultramer oligo repair template
	1. Add 0.65ul 50uM dyp-10 ssDNA Ultramer oligo repair template to RNP mix for ~3uM final concentration
	2. Spin at 13,000RPM for 5 minutes

*Notes*:

* Always use barrier/filter tips to prevent contamination.
* Always use RNAse-free microfuge tubes, tips, and water.
* Work in an RNAse-free environment, wear full PPE, and change gloves often.
* Always keep resuspended RNA on ice to prevent degradation.

*\*\*When screening, dumpy worms are usually dpy-10 homozygotes, which will need to be crossed out.*
LB Agar

1. Add to 1000 ml in a 2 L flask (agar will not go into solution):
	1. 10 g Bacto-tryptone
	2. 5 g Yeast extract
	3. 10 g NaCl
	4. 20 g Agar
2. Sterilize by autoclaving. Keep stir bar in flask during autoclaving.
3. After autoclaving, place flask on stir plate and stir slowly. Cool so that you can touch flask with bare hands (around 55°C). If necessary, add 1 ml antibiotic (if you add it while the media is still hot the antibiotic will not work) and mix. Pour into petri dishes.
4. Mark petri dishes with marker to indicate what types of plates they are. See below.
5. When agar has solidified, turn plates over to dry upside down for one day. Ask someone to bag them and put in the cold room.

**Plates cannot stay indefinitely at room temperature so make sure you remind someone to bag the plates!**
HeLa Cell Immunostaining

1. The day before, cells need to be split. I usually wait until they are ~80% confluent, and then split them into chambered coverglass wells 50uL cells: 450uL media. Be sure to spin off trypsin so they adhere better.

2. Allow to grow overnight in 37C CO2 incubator, about 18-26 hours.

3. Remove chamber from incubator and check cells on scope to make sure they are adhered and look healthy.

4. Carefully aspirate off media from corner of well (avoid aspirating off too many cells).

5. Immediately add 500uL pre-extraction buffer (0.2% Triton X-100, 100mM Pipes pH 6.8, 1mM MgCl2, 5mM EGTA), incubate 1 min @ RT.

6. Carefully aspirate off buffer, add 200uL fixative (3.2% Paraformaldehyde in PBS), incubate 10min @ RT.

7. Aspirate off fixative, wash cells 1X in 1X PBS. Block with 200uL 3% BSA (30mg/mL in PBS) for 1 hour @ RT.

8. Aspirate off block, add ~100uL of primary Ab (3% BSA, 0.1% TX-100, Bub1 1:500, DM1A 1:1000, & RNase A .08mg/mL in PBS). Incubate chamber (wrapped in parafilm) overnight @4C.

9. The next morning, wash cells 3X in PBS+0.01% TX-100 for 5min @ RT.

10. Add secondary AB (goat a-mouse 488 1:300, goat a-rabbit 594 1:300, Draq5 1:1000, 3% BSA, 0.1% TX-100 in PBS) for 1hr @ RT in the dark.

11. Wash cells 3X in PBS+0.01% TX-100 for 5min @ RT.

12. Mount with 20uL mounting media (1X PBS, 70% glycerol).

13. If not imaging immediately, store chamber @4C wrapped in parafilm.
Protocols

List of different protocols used in the lab.

Immunofluorescence

PCR from Worm Lysate

PCR from Genomic Prep

Live Imaging/Embryo Microscopy

coCRISPR RNP Mix Assembly

Bacterial Transformation (10Beta)

Feeding RNAi

Scoring Apoptosis

Freezing Worms

Bleaching Worms

Worm Viability Assay

Worm Viability Spreadsheet

Worm Genomic Prep

Trizol Extraction of RNA

Ethanol Precipitation

Co-immunoprecipitation

Worm Lysate Prep & Western Blot Analysis

HeLa Cell Immunostaining

10Beta Competent Cell Prep
Directions for Autoclave

## Autoclaving Dishes

* **Beakers** and **graduated cylinders** are autoclaved uncovered.
* **Bottles** are autoclaved with their caps on but not completely screwed on.
	+ Rule of thumb: screw cap on tight and then loosen half a turn.
* **Flasks** are capped with foil.

* Put autoclave tape on the cap or foil of both bottles and flasks so any users know that they are sterile.
* Autoclave time for dishes (choose dry run):
	+ sterilization ~ 20 minutes
	+ dry ~ 20 minutes
* Put dishes back in their cabinet after autoclaving, once cooled.

## Autoclaving Liquids or Fluids

* **Liquids in bottles** are autoclaved with their caps on but not completely screwed on.
* **Liquids in flasks** are autoclaves with a foil cap.
* Put autoclave tape on the cap or foil of both bottles and flasks so any users know that they are sterile.
* Autoclave time for fluids (choose fluid run):
	+ sterilization ~40 minutes
* **Remember to reset jacket pressure after a fluid run!**
* Put bottles on the shelf after autoclaving.
* Place flasks with media on a stir plate after autoclaving to let cool before adding antibiotic or pouring plates.
Synthetic Complete Dropout Media

1. 26.7 g Dropout Base (DOB)
2. + appropriate amino acid supplement mixture
	* SC-Leu: 1.83 g/L
	* SC-Leu-Trp: 1.74 g/L
	* SC-Leu-Trp-His: 1.66 g/L

+ 100 g Adenine Sulfate

1. Millipore water to 1 liter

## Plates Containing 3-Amino- 1,2,4- Triazole (3AT)

1. Follow the previous recipe for SC-Leu-Trp-His
2. Cool to approximately 65 °C
3. Add 3AT as powder, stir to dissolve, then pour plates without further adjusting the pH
4. Make plates with 10 mM, 25 mM, 75 mM, and 100 mM 3At to test auto-activation of DB fusion
Freezing Worms

1. Make sure worms are clean: no contaminating bacteria or mold! Chunk clean plate of relevant genotype to six NGM plates with spread or spotted OP50. If you are freezing a balanced strain, pick 20-25 of the balanced genotype to each plate to make sure that your balanced genotype is well represented in the frozen population.
2. Worms should be harvested off plates one day after the plates have starved so that the population of worms are mostly L1s and L2s with no food in their gut (this reduces the viability of frozen worm stocks). Add ~2mls of S basal to each plate and swirl briefly to dislodge worms. Remove S basal to 15ml conical tube.
3. Spin down worms 15-30 sec at 2000RPM in clinical centrifuge. Remove all but 1.5mls S basal and add 1.5mls worm freezing solution. Mix and aliquot 600ul into 5 sterile Nunc vials. Insert colored caps into two of the vials (write strain number on top), and label all the tubes with the strain number AND genotype.
4. Freeze all five tubes at -80° overnight. Pull out one of the nalgene tubes and let thaw. Pour out onto a spread NGM plate and let dry. This is your test thaw. **If enough of the worms do not survive the freezing you will need to refreeze the strain**. If the test thaw is successful, enter the strain in the worm lab collection; it will be assigned the BHL strain designation (i.e. BHL1). Three of the tubes will go in to strain collection at -80°. One of these will be the Nunc tube with a colored cap insert on which the BHL strain designation will be written. The other Nunc tube will be placed in the liquid nitrogen container in the basket, cane and position indicated in the strain collection.
5. If you need a strain from the collection, check with other members of the lab to see if it is already out. If you pull a strain out of the -80° collection, you are responsible for maintaining it for a year so that if anyone else needs it, they can go to you and not have to pull it out of the strain collection. If you pull out the last strain from the -80° collection, you are responsible for freezing down another three copies to replace it. Strains are to be pulled out of the liquid nitrogen collection only if all strains from the -80° collection are gone or otherwise compromised.
TRIzol method for RNA isolation

1. chunk one N2 (or mutant) 100mm plate to 4-6 100mm plates seeded with OP50
2. let grow at 20° (unless doing a prep on a temperature sensitive mutant) until the plate is dense and most of the OP50 is gone (most of the animals should be adults and L4s); this should take 2-4 days depending on the density of the plate that was chunked
3. wash worms off these plates with M9 media into a 1.5ml eppendorf tube (you may need to spin the tube down several times to get all the washes in one tube)
4. after getting all the worms in one tube, wash 1X with 1ml of M9 media
5. add 800ul TRIzol
6. homogenize for 60 sec (by vortexing) - add 160ul chloroform (1/5 volume of Trizol)
7. mix by vortexing for 15 sec
8. incubate for 5 min at room temperature
9. centrifuge at 12,000 g for 15 min at 4°C
10. transfer the aqueous phase into a new tube
11. add 500ul isopropanol
12. centrifuge at max for 10 min in the cold room
13. wash the pellet with 500ul 70 % ethanol
14. centrifuge at max for 2 min in the cold room
15. air-dry the pellet for 5-10 min - disolve the pellet in 50-100 ml DEPC-H2O
16. OD prep at 260nm, you’ll need 1ug for RT-PCR (prepping 2 100mm plates of N2 worms typically give total RNA at a concentration of .5ug/ul)
10X PBS

1. To 800 ml distilled H2O add:
	* 80 g NaCl
	* 2.0 g KCl
	* 14.4 g Na2HPO4
	* 2.4 g KH2PO4
2. Adjust pH to 7.4
3. Adjust final volume to 1L and re-mix
4. Label and pour into 1 L bottle
5. Autoclave for sterilization

## **Making 1X PBST**

1. Make 10X PBS as directed above
2. Once cool add to 10L Carboy
3. Add 20 ml Tween
4. Fill to final volume of 10L and mix well

## Making High Salt 1X PBST

1. Follow directions for 10X PBST except add an additional 146.1 g NaCl
2. Once cool add to 10L Carboy
3. Add 20 ml Tween
4. Fill to final volume of 10L and mix well
Bhalla Lab Wiki

**Welcome to the Bhalla Lab Wiki!**
Here you will find details about protocols used in the lab. Feel free to add tips and make changes that better the protocols.

Special thanks to Sagel Ejantkar for creating this website and transcribing all the protocols from the Bhalla binder!!!

the admin dashboard for more tips.

## Guide to the website

Lab On-boarding and etiquette

Protocols

Media and Plates

Lab Map

Antibody Guide

*Need help building out this community?*

* Getting Started
* How to Contribute
* Managing your new community
* Guides
* All Help articles

You can also be part of the larger Fandom family of communities. Visit Fandom's Community Central!
Stocked Reagents

## **1 M CaCl2**

1. Dissolve 14.7 g per 100 mls distilled H2O
2. Autoclave.

## **1 M MgCl2**

1. Dissolve 20.3 g per 100 mls distilled H2O
2. Autoclave.

## **1 M NaCl**

1. Dissolve 5.8 g per 100 mls distilled H2O
2. Autoclave.

## **1 M MgSO4**

1. Dissolve 12.0 g per 100 mls distilled H2O
2. Autoclave

## **5 mg/ml Cholesterol in EtOH**

1. Dissolve 1 g cholesterol in 200 ml EtOH.
2. Filter sterilize. *DO NOT AUTOCLAVE!*

## **10 N NaOH**

1. Dissolve 40 g per 100 mls distilled H2O
2. Store in 50 ml conical tubes.
3. *DO NOT AUTOCLAVE!*

## **10 N KOH**

1. dissolve 56.1 per 100 ml distilled H2O
2. Store in 50 ml conical tubes.
3. *DO NOT AUTOCLAVE!*
Bacterial Transformation (10-Beta Cells)

These cells are designed for quick transformation and transformation efficiency actually drops if you increase the time of ice incubation steps.

1. Thaw aliquot of 10B cells on ice

2. Add plasmid (generally 1uL)

3. Incubate on ice 2 minutes.

4. Heatshock 42°C for 30 seconds.

5. Incubate on ice 1 minute.

6. Add 300uL SOC media (sterile technique) to cells.

7. Mix well and plate cells on LB + Carb plate.

* NOTE: If you are using KAN or other resistance marker, you need to shake the cells in SOC for 30 minutes prior to plating, but this step is not necessary for carb/amp resistance plasmids.