Dehytion3

so much

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(32);

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wlll turnn too whisperr inn yourr ear,,<<

p0And ~~*you*~~ know what they say might hurt ~~*you*~~，L

1. ///And//////////////////////////\\\\\\\\\\\\\\\\\\\\\\\\\\\\
2. //////////////you/////////////\\\\\\\\\\\\\\\\\\\\\\\\\\\\
3. //////////////////////////know//\\\\\\\\\\\\\\\\\\\\\\\\\\\\
4. ///////////////////////////////\\\\\\\\\that\\\\\\\\\\\\\
5. ///////////////////////////////\it\\\\\\\\\\\\\\\\\\\\\\\
6. ///////////////////means////////\\\\\\\\\\\\\\\\\\\\\\\\\\\\

* so much
* so much
* so much
	+ so much
	+ so much
	+ ~~so much~~
		- ~~so much~~
		- ~~so much~~
		- ~~And~~
		- ~~you don't~~
		- ~~even feel~~
		- ~~a thing，~~
		- ~~so much~~

~~so much~~

* And you don't.
	+ **解析失败 (语法错误): {\displaystyle \ce{^{19^{15}\_{19}n^{-}\_{-}Th+}}**
		- * ~~so much~~
			* ~~so much~~
				+ ~~so much~~

                have lost it all(layout\_width="

```
var Map;
 function map(){
 this.mapp=null;
 this.makemap=function(){
 this.mapp=document.createElement ("div");
 this.mapp.classN"!";
 document.body.appendChild(this.mapp );
 }
 }

```
"

## have lost it all

~~so much]~~

* [19][15][19]
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		- \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\6
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					- ~~so much~~
					- ~~so muchmuchmuchmuchmuchmuch~~
				+ ~~so much~~
				+ ~~so much~~

* ~~so much~~
* ~~so much~~
* ~~so much~~
	+ so much
	+ so much
	+ so much
		- so much
		- so much
		- so much
			* so much
			* so much
			* so much

* so much
* so much
* so much

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I'm sorry that I didn't reach my goal and I didn't finish my task. I am useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless uselessuseless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless, useless useless useless useless

Self destruction.

because

!

.

w

*Corynebacterium accolens* is the predominant species of the healthy human nasal microbiota, and its relative abundance is decreased in the context of chronic rhinosinusitis (CRS). This study aimed to evaluate the antimicrobial potential of *C. accolens* isolated from a healthy human nasal cavity against planktonic and biofilm growth of *Staphylococcus aureus* (*S. aureus*) and methicillin-resistant *S. aureus* (MRSA) clinical isolates (CIs) from CRS patients. Nasal swabs from twenty non-CRS control subjects were screened for the presence of *C. accolens* using microbiological and molecular techniques. *C. accolens* CIs and their culture supernatants were tested for their antimicrobial activity against eight *S. aureus* and eight MRSA 4CIs and *S. aureus* ATCC25923. The anti-biofilm potential of *C. accolens* cell-free culture supernatants (CFCSs) on *S. aureus* biofilms was also assessed. Of the 20 nasal swabs, 10 *C. accolens* CIs were identified and confirmed with *rpoB* gene sequencing. All isolates showed variable antimicrobial activity against eight out of 8 *S. aureus* and seven out of eight MRSA CIs. Culture supernatants from all *C. accolens* CIs exhibited a significant dose-dependent antibacterial activity (*p* < 0.05) against five out of five representative *S. aureus* and MRSA CIs. This inhibition was abolished after proteinase K treatment. *C. accolens* supernatants induced a significant reduction in metabolic activity and biofilm biomass of *S. aureus* and MRSA CIs compared to untreated growth control (*p* < 0.05). *C. accolens* exhibited antimicrobial activity against *S. aureus* and MRSA CIs in both planktonic and biofilm forms and holds promise for the development of innovative probiotic therapies to promote sinus health.

chronic rhinosinusitis, *Corynebacterium accolens*, microbiota, sinusDisruption of the human nasal microbiome homeostasis is found in patients with chronic rhinosinusitis (CRS). CRS is an inflammatory disorder of the mucosa of the nasal cavity and paranasal sinuses, characterized by various clinical manifestations including sinus/facial pain, nasal congestion, rhinorrhoea, post-nasal discharge, and a reduced sense of smell for a minimum of 12 weeks duration [1]. Whilst the aetiology of CRS is thought to be multifactorial, disruption of the microbial community residing in the sinuses, termed dysbiosis, has recently been implicated in CRS pathophysiology, in particular in more severe patients [2]. Dysbiosis is generally described as an imbalance of pathologic and commensal bacteria, which are involved in the protection against overgrowth of pathobionts or potentially disease-causing organisms [3,4]. In CRS patients, the microbiome is characterised by a decrease in the relative abundance of *Corynebacterium* and an expansion of pathogenic bacteria including *Staphylococcus*, *Haemophilus*, *Moraxella* and *Enterobacteriacea* [5]. Among pathogenic species, *Staphylococcus aureus* is the most frequently isolated species in patients with CRS [6]. Furthermore, exacerbations of CRS due to *S. aureus* and methicillin-resistant *S. aureus* (MRSA) have been reported in severe recalcitrant disease, leading to immune dysregulation, barrier dysfunction, biofilm formation, and worse clinical outcomes [7,8,9].

Several studies have shown that microbes that typically exist within the mucosa of the nasal cavity compete amongst themselves and inhibit the growth of competitors either by releasing antagonistic substances or by limiting access to nutrients from the surrounding environment [10,11]. For example, *Corynebacterium accolens*, a common commensal nasal species secretes the enzyme triacylglycerol lipase (LipS1) that degrades triacylglycerol to produce free fatty acids that interfere with the growth of *Streptococcus pneumoniae* in the nasal cavity [12,13].

Similarly, probiotic bacteria have been acknowledged as a potential novel treatment in various diseases of the gut linked to dysbiosis, as they can interfere with the growth of pathogenic organisms and provide a host-beneficial advantage [14]. In the context of CRS, manipulation

of the sinonasal microbiome has been recognized as an innovative strategy to promote the re- health

it …! perfect!!!!!

!!!!!!

!!!!!

!!!!!!!

!!!!

Self destruction.

1. ↑ I *am falling,*

I am ~~fading,~~**解析失败 (语法错误): {\displaystyle \operatorname{argsh}2\\operatorname{argch}7\left\vert e \right\vert \liminf>}**
2. ↑ !!!!!!!