Mass extinction 3: Permian–Triassic extinction event

The **Permian–Triassic extinction event**, also known as the **P–Tr extinction**, the **P–T extinction**, the **End-Permian Extinction**, and colloquially as the **Great Dying**, formed the boundary between the Permian and Triassic geologic periods, as well as between the Paleozoic and Mesozoic eras, approximately 251.9 million years ago. It is the Earth's most severe known extinction event, with around 81% of all marine species and 70% of terrestrial vertebrate species becoming extinct. It was the largest known mass extinction of insects. Some 57% of all biological families and 83% of all genera became extinct.

There is evidence for one to three distinct pulses, or phases, of extinction. Potential causes for those pulses include one or more large meteor impact events, massive volcanic eruptions (such as the Siberian Traps), and climate change brought on by large releases of underwater methane, methane-producing microbes, or combustion of fossil fuels.

The speed of the recovery from the extinction is disputed. Some scientists estimate that it took 10 million years (until the Middle Triassic), due both to the severity of the extinction and because grim conditions returned periodically for another 5 million years. However, studies in Bear Lake County, near Paris, Idaho, showed a relatively quick rebound in a localized Early Triassic marine ecosystem, taking around 2 million years to recover, suggesting that the impact of the extinction may have been felt less severely in some areas than others. Until 2000, it was thought that rock sequences spanning the Permian–Triassic boundary were too few and contained too many gaps for scientists to reliably determine its details. However, it is now possible to date the extinction with millennial precision. U–Pb zircon dates from five volcanic ash beds from the Global Stratotype Section and Point for the Permian–Triassic boundary at Meishan, China, establish a high-resolution age model for the extinction – allowing exploration of the links between global environmental perturbation, carbon cycle disruption, mass extinction, and recovery at millennial timescales. The extinction occurred between 251.941 ± 0.037 and 251.880 ± 0.031 Ma ago, a duration of 60 ± 48 ka. A large (approximately 0.9%), abrupt global decrease in the ratio of the stable isotope 13

C to that of 12

C, coincides with this extinction, and is sometimes used to identify the Permian–Triassic boundary in rocks that are unsuitable for radiometric dating. Further evidence for environmental change around the P–Tr boundary suggests an 8 °C (14 °F) rise in temperature, and an increase in CO

2 levels by 2000 ppm (for comparison, the concentration immediately before the industrial revolution was 280 ppm, and the amount today is about 410 ppm). There is also evidence of increased ultraviolet radiation reaching the earth, causing the mutation of plant spores.

It has been suggested that the Permian–Triassic boundary is associated with a sharp increase in the abundance of marine and terrestrial fungi, caused by the sharp increase in the amount of dead plants and animals fed upon by the fungi. For a while this "fungal spike" was used by some paleontologists to identify the Permian–Triassic boundary in rocks that are unsuitable for radiometric dating or lack suitable index fossils, but even the proposers of the fungal spike hypothesis pointed out that "fungal spikes" may have been a repeating phenomenon created by the post-extinction ecosystem in the earliest Triassic. The very idea of a fungal spike has been criticized on several grounds, including: *Reduviasporonites*, the most common supposed fungal spore, may be a fossilized alga; the spike did not appear worldwide; and in many places it did not fall on the Permian–Triassic boundary. The reduviasporonites may even represent a transition to a lake-dominated Triassic world rather than an earliest Triassic zone of death and decay in some terrestrial fossil beds. Newer chemical evidence agrees better with a fungal origin for *Reduviasporonites*, diluting these critiques.

Uncertainty exists regarding the duration of the overall extinction and about the timing and duration of various groups' extinctions within the greater process. Some evidence suggests that there were multiple extinction pulses or that the extinction was spread out over a few million years, with a sharp peak in the last million years of the Permian. Statistical analyses of some highly fossiliferous strata in Meishan, Zhejiang Province in southeastern China, suggest that the main extinction was clustered around one peak. Recent research shows that different groups became extinct at different times; for example, while difficult to date absolutely, ostracod and brachiopod extinctions were separated by 670,000 to 1.17 million years. In a well-preserved sequence in east Greenland, the decline of animals is concentrated in a period 10,000 to 60,000 years long, with plants taking an additional several hundred thousand years to show the full impact of the event.

An older theory, still supported in some recent papers, is that there were two major extinction pulses 9.4 million years apart, separated by a period of extinctions well above the background level, and that the final extinction killed off only about 80% of marine species alive at that time while the other losses occurred during the first pulse or the interval between pulses. According to this theory one of these extinction pulses occurred at the end of the Guadalupian epoch of the Permian. For example, all but one of the surviving dinocephalian genera died out at the end of the Guadalupian, as did the Verbeekinidae, a family of large-size fusuline foraminifera. The impact of the end-Guadalupian extinction on marine organisms appears to have varied between locations and between taxonomic groups — brachiopods and corals had severe losses.

### Marine organisms

Marine invertebrates suffered the greatest losses during the P–Tr extinction. Evidence of this was found in samples from south China sections at the P–Tr boundary. Here, 286 out of 329 marine invertebrate genera disappear within the final two sedimentary zones containing conodonts from the Permian. The decrease in diversity was probably caused by a sharp increase in extinctions, rather than a decrease in speciation.

The extinction primarily affected organisms with calcium carbonate skeletons, especially those reliant on stable CO2 levels to produce their skeletons. These organisms were susceptible to the effects of the ocean acidification that resulted from increased atmospheric CO2.

Among benthic organisms the extinction event multiplied background extinction rates, and therefore caused maximum species loss to taxa that had a high background extinction rate (by implication, taxa with a high turnover). The extinction rate of marine organisms was catastrophic.

Surviving marine invertebrate groups included articulate brachiopods (those with a hinge), which had undergone a slow decline in numbers since the P–Tr extinction; the Ceratitida order of ammonites; and crinoids ("sea lilies"), which very nearly became extinct but later became abundant and diverse.

The groups with the highest survival rates generally had active control of circulation, elaborate gas exchange mechanisms, and light calcification; more heavily calcified organisms with simpler breathing apparatuses suffered the greatest loss of species diversity. In the case of the brachiopods, at least, surviving taxa were generally small, rare members of a formerly diverse community.

The ammonoids, which had been in a long-term decline for the 30 million years since the Roadian (middle Permian), suffered a selective extinction pulse 10 million years before the main event, at the end of the Capitanian stage. In this preliminary extinction, which greatly reduced disparity, or the range of different ecological guilds, environmental factors were apparently responsible. Diversity and disparity fell further until the P–Tr boundary; the extinction here (P–Tr) was non-selective, consistent with a catastrophic initiator. During the Triassic, diversity rose rapidly, but disparity remained low.

The range of morphospace occupied by the ammonoids, that is, their range of possible forms, shapes or structures, became more restricted as the Permian progressed. A few million years into the Triassic, the original range of ammonoid structures was once again reoccupied, but the parameters were now shared differently among clades.

### Terrestrial invertebrates

The Permian had great diversity in insect and other invertebrate species, including the largest insects ever to have existed. The end-Permian is the largest known mass extinction of insects; according to some sources, it is the *only* insect mass extinction. Eight or nine insect orders became extinct and ten more were greatly reduced in diversity. Palaeodictyopteroids (insects with piercing and sucking mouthparts) began to decline during the mid-Permian; these extinctions have been linked to a change in flora. The greatest decline occurred in the Late Permian and was probably not directly caused by weather-related floral transitions.

Most fossil insect groups found after the Permian–Triassic boundary differ significantly from those before: Of Paleozoic insect groups, only the Glosselytrodea, Miomoptera, and Protorthoptera have been discovered in deposits from after the extinction. The caloneurodeans, monurans, paleodictyopteroids, protelytropterans, and protodonates became extinct by the end of the Permian. In well-documented Late Triassic deposits, fossils overwhelmingly consist of modern fossil insect groups.

### Terrestrial plants

#### Plant ecosystem response[edit]

The geological record of terrestrial plants is sparse and based mostly on pollen and spore studies. Plants are relatively immune to mass extinction, with the impact of all the major mass extinctions "insignificant" at a family level.[*dubious – discuss*] Even the reduction observed in species diversity (of 50%) may be mostly due to taphonomic processes. However, a massive rearrangement of ecosystems does occur, with plant abundances and distributions changing profoundly and all the forests virtually disappearing; the Palaeozoic flora scarcely survived this extinction.

At the P–Tr boundary, the dominant floral groups changed, with many groups of land plants entering abrupt decline, such as *Cordaites* (gymnosperms) and *Glossopteris* (seed ferns). Dominant gymnosperm genera were replaced post-boundary by lycophytes—extant lycophytes are recolonizers of disturbed areas.

Palynological or pollen studies from East Greenland of sedimentary rock strata laid down during the extinction period indicate dense gymnosperm woodlands before the event. At the same time that marine invertebrate macrofauna declined, these large woodlands died out and were followed by a rise in diversity of smaller herbaceous plants including Lycopodiophyta, both Selaginellales and Isoetales. Later, other groups of gymnosperms again become dominant but again suffered major die offs. These cyclical flora shifts occurred a few times over the course of the extinction period and afterwards. These fluctuations of the dominant flora between woody and herbaceous taxa indicate chronic environmental stress resulting in a loss of most large woodland plant species. The successions and extinctions of plant communities do not coincide with the shift in δ13C values, but occurred many years after. The recovery of gymnosperm forests took 4–5 million years.

#### Coal gap[edit]

No coal deposits are known from the Early Triassic, and those in the Middle Triassic are thin and low-grade. This "coal gap" has been explained in many ways. It has been suggested that new, more aggressive fungi, insects and vertebrates evolved and killed vast numbers of trees. These decomposers themselves suffered heavy losses of species during the extinction and are not considered a likely cause of the coal gap. It could simply be that all coal-forming plants were rendered extinct by the P–Tr extinction, and that it took 10 million years for a new suite of plants to adapt to the moist, acid conditions of peat bogs. Abiotic factors (factors not caused by organisms), such as decreased rainfall or increased input of clastic sediments, may also be to blame.

On the other hand, the lack of coal may simply reflect the scarcity of all known sediments from the Early Triassic. Coal-producing ecosystems, rather than disappearing, may have moved to areas where we have no sedimentary record for the Early Triassic. For example, in eastern Australia a cold climate had been the norm for a long period, with a peat mire ecosystem adapted to these conditions. Approximately 95% of these peat-producing plants went *locally* extinct at the P–Tr boundary; coal deposits in Australia and Antarctica disappear significantly *before* the P–Tr boundary.

### Terrestrial vertebrates

There is enough evidence to indicate that over two thirds of terrestrial labyrinthodont amphibians, sauropsid ("reptile") and therapsid ("proto-mammal") families became extinct. Large herbivores suffered the heaviest losses.

All Permian anapsid reptiles died out except the procolophonids (although testudines have *morphologically*-anapsid skulls, they are now thought to have separately evolved from diapsid ancestors). Pelycosaurs died out before the end of the Permian. Too few Permian diapsid fossils have been found to support any conclusion about the effect of the Permian extinction on diapsids (the "reptile" group from which lizards, snakes, crocodilians, and dinosaurs (including birds) evolved).

The groups that survived suffered extremely heavy losses of species, and some terrestrial vertebrate groups very nearly became extinct at the end of the Permian. Some of the surviving groups did not persist for long past this period, but others that barely survived went on to produce diverse and long-lasting lineages. However, it took 30 million years for the terrestrial vertebrate fauna to fully recover both numerically and ecologically.

### Possible explanations

An analysis of marine fossils from the Permian's final Changhsingian stage found that marine organisms with low tolerance for hypercapnia (high concentration of carbon dioxide) had high extinction rates, and the most tolerant organisms had very slight losses.

The most vulnerable marine organisms were those that produced calcareous hard parts (from calcium carbonate) and had low metabolic rates and weak respiratory systems, notably calcareous sponges, rugose and tabulate corals, calcite-depositing brachiopods, bryozoans, and echinoderms; about 81% of such genera became extinct. Close relatives without calcareous hard parts suffered only minor losses, such as sea anemones, from which modern corals evolved. Animals with high metabolic rates, well-developed respiratory systems, and non-calcareous hard parts had negligible losses except for conodonts, in which 33% of genera died out.

This pattern is consistent with what is known about the effects of hypoxia, a shortage but not total absence of oxygen. However, hypoxia cannot have been the only killing mechanism for marine organisms. Nearly all of the continental shelf waters would have had to become severely hypoxic to account for the magnitude of the extinction, but such a catastrophe would make it difficult to explain the very selective pattern of the extinction. Mathematical models of the Late Permian and Early Triassic atmospheres show a significant but protracted decline in atmospheric oxygen levels, with no acceleration near the P–Tr boundary. Minimum atmospheric oxygen levels in the Early Triassic are never less than present-day levels and so the decline in oxygen levels does not match the temporal pattern of the extinction.

Marine organisms are more sensitive to changes in CO

2 (carbon dioxide) levels than terrestrial organisms for a variety of reasons. CO

2 is 28 times more soluble in water than is oxygen. Marine animals normally function with lower concentrations of CO

2 in their bodies than land animals, as the removal of CO

2 in air-breathing animals is impeded by the need for the gas to pass through the respiratory system's membranes (lungs' alveolus, tracheae, and the like), even when CO

2 diffuses more easily than oxygen. In marine organisms, relatively modest but sustained increases in CO

2 concentrations hamper the synthesis of proteins, reduce fertilization rates, and produce deformities in calcareous hard parts. In addition, an increase in CO

2 concentration is inevitably linked to ocean acidification, consistent with the preferential extinction of heavily calcified taxa and other signals in the rock record that suggest a more acidic ocean. The decrease in ocean pH is calculated to be up to 0.7 units.

It is difficult to analyze extinction and survival rates of land organisms in detail because few terrestrial fossil beds span the Permian–Triassic boundary. Triassic insects are very different from those of the Permian, but a gap in the insect fossil record spans approximately 15 million years from the late Permian to early Triassic. The best-known record of vertebrate changes across the Permian–Triassic boundary occurs in the Karoo Supergroup of South Africa, but statistical analyses have so far not produced clear conclusions. However, analysis of the fossil river deposits of the floodplains indicate a shift from meandering to braided river patterns, indicating an abrupt drying of the climate. The climate change may have taken as little as 100,000 years, prompting the extinction of the unique *Glossopteris* flora and its herbivores, followed by the carnivorous guild. End-Permian extinctions did not occur at an instantaneous time horizon; particularly, floral extinction was delayed in time.

## Biotic recovery

In the wake of the extinction event, the ecological structure of present-day biosphere evolved from the stock of surviving taxa. In the sea, the "Modern Evolutionary Fauna" became dominant over elements of the "Palaeozoic Evolutionary Fauna". Typical taxa of shelly benthic faunas were now bivalves, snails, sea urchins and Malacostraca, whereas bony fishes and marine reptiles diversified in the pelagic zone. On land, dinosaurs and mammals arose in the course of the Triassic. The profound change in the taxonomic composition was partly a result of the selectivity of the extinction event, which affected some taxa (e.g., brachiopods) more severely than others (e.g., bivalves). However, recovery was also differential between taxa. Some survivors became extinct some million years after the extinction event without having rediversified (dead clade walking, e.g. the snail family Bellerophontidae, whereas others rose to dominance over geologic times (e.g., bivalves).

## Theories about cause

Pinpointing the exact causes of the Permian–Triassic extinction event is difficult, mostly because it occurred over 250 million years ago, and since then much of the evidence that would have pointed to the cause has been destroyed or is concealed deep within the Earth under many layers of rock. The sea floor is also completely recycled every 200 million years by the ongoing process of plate tectonics and seafloor spreading, leaving no useful indications beneath the ocean.

Yet, scientists have gathered significant evidence for causes, and several mechanisms have been proposed. The proposals include both catastrophic and gradual processes (similar to those theorized for the Cretaceous–Paleogene extinction event).

* The *catastrophic* group includes one or more large bolide impact events, increased volcanism, and sudden release of methane from the sea floor, either due to dissociation of methane hydrate deposits or metabolism of organic carbon deposits by methanogenic microbes.
* The *gradual* group includes sea level change, increasing anoxia, and increasing aridity.

Any hypothesis about the cause must explain the selectivity of the event, which affected organisms with calcium carbonate skeletons most severely; the long period (4 to 6 million years) before recovery started, and the minimal extent of biological mineralization (despite inorganic carbonates being deposited) once the recovery began.

#### Possible impact sites[edit]

Several possible impact craters have been proposed as the site of an impact causing the P–Tr extinction, including the 250 km (160 mi) Bedout structure off the northwest coast of Australia and the hypothesized 480 km (300 mi) Wilkes Land crater of East Antarctica. An impact has not been proved in either case, and the idea has been widely criticized. The Wilkes Land sub-ice geophysical feature is of very uncertain age, possibly later than the Permian–Triassic extinction.

The 40 km (25 mi) Araguainha crater in Brazil has been most recently dated to 254.7 ± 2.5 million years ago, overlapping with estimates for the Permo-Triassic boundary. Much of the local rock was oil shale. The estimated energy released by the Araguainha impact is insufficient to have directly caused the global mass extinction, but the colossal local earth tremors would have released huge amounts of oil and gas from the shattered rock. The resulting sudden global warming might have precipitated the Permian–Triassic extinction event.

A 2017 paper by Rampino, Rocca and Presser (after a 1992 abstract by Rampino) noted the discovery of a circular gravity anomaly near the Falkland Islands which might correspond to an impact crater with a diameter of 250 km (160 mi), as supported by seismic and magnetic evidence. Estimates for the age of the structure range up to 250 million years old. This would be substantially larger than the well-known 180 km (110 mi) Chicxulub impact crater associated with a later extinction. However, Dave McCarthy and colleagues from the British Geological Survey illustrated that the gravity anomaly is not circular and also that the seismic data presented by Rocca, Rampino and Baez Presser did not cross the proposed crater or provide any evidence for an impact crater.

### Volcanism[edit]

The final stages of the Permian had two flood basalt events. A smaller one, the Emeishan Traps in China, occurred at the same time as the end-Guadalupian extinction pulse, in an area close to the equator at the time. The flood basalt eruptions that produced the Siberian Traps constituted one of the largest known volcanic events on Earth and covered over 2,000,000 square kilometres (770,000 sq mi) with lava. The date of the Siberian Traps eruptions and the extinction event are in good agreement.

The Emeishan and Siberian Traps eruptions may have caused dust clouds and acid aerosols, which would have blocked out sunlight and thus disrupted photosynthesis both on land and in the photic zone of the ocean, causing food chains to collapse. The eruptions may also have caused acid rain when the aerosols washed out of the atmosphere. That may have killed land plants and molluscs and planktonic organisms which had calcium carbonate shells. The eruptions would also have emitted carbon dioxide, causing global warming. When all of the dust clouds and aerosols washed out of the atmosphere, the excess carbon dioxide would have remained and the warming would have proceeded without any mitigating effects.

The Siberian Traps had unusual features that made them even more dangerous. Pure flood basalts produce fluid, low-viscosity lava and do not hurl debris into the atmosphere. It appears, however, that 20% of the output of the Siberian Traps eruptions was pyroclastic (consisted of ash and other debris thrown high into the atmosphere), increasing the short-term cooling effect. The basalt lava erupted or intruded into carbonate rocks and into sediments that were in the process of forming large coal beds, both of which would have emitted large amounts of carbon dioxide, leading to stronger global warming after the dust and aerosols settled.

In January 2011, a team, led by Stephen Grasby of the Geological Survey of Canada—Calgary, reported evidence that volcanism caused massive coal beds to ignite, possibly releasing more than 3 trillion tons of carbon. The team found ash deposits in deep rock layers near what is now the Buchanan Lake Formation. According to their article, "coal ash dispersed by the explosive Siberian Trap eruption would be expected to have an associated release of toxic elements in impacted water bodies where fly ash slurries developed.... Mafic megascale eruptions are long-lived events that would allow significant build-up of global ash clouds." In a statement, Grasby said, "In addition to these volcanoes causing fires through coal, the ash it spewed was highly toxic and was released in the land and water, potentially contributing to the worst extinction event in earth history." In 2013, a team led by Q.Y. Yang reported the total amounts of important volatiles emitted from the Siberian Traps are 8.5 × 107 Tg CO2, 4.4 × 106 Tg CO, 7.0 × 106 Tg H2S and 6.8 × 107 Tg SO2, the data support a popular notion that the end-Permian mass extinction on the Earth was caused by the emission of enormous amounts of volatiles from the Siberian Traps into the atmosphere.

In 2015, evidence and a timeline indicated the extinction was caused by events in the large igneous province of the Siberian Traps.

In 2020 scientists reconstructed the mechanisms that led to the extinction event in a biogeochemical model, showed the consequences of the greenhouse effect on the marine environment and reported that the mass extinction can be traced back to volcanic CO2 emissions. Further evidence – based on paired coronene-mercury spikes – for a volcanic combustion cause of the mass extinction was published in 2020.

### Methane hydrate gasification

Main article: Clathrate gun hypothesis

Further information: Arctic methane release

Scientists have found worldwide evidence of a swift decrease of about 1% in the 13C/12C isotope ratio in carbonate rocks from the end-Permian. This is the first, largest, and most rapid of a series of negative and positive excursions (decreases and increases in 13C/12C ratio) that continues until the isotope ratio abruptly stabilised in the middle Triassic, followed soon afterwards by the recovery of calcifying life forms (organisms that use calcium carbonate to build hard parts such as shells).

A variety of factors may have contributed to this drop in the 13C/12C ratio, but most turn out to be insufficient to account fully for the observed amount:

* Gases from volcanic eruptions have a 13C/12C ratio about 0.5 to 0.8% below standard (δ13C2 about −0.5 to −0.8%), but an assessment made in 1995 concluded that the amount required to produce a reduction of about 1.0% worldwide requires eruptions greater by orders of magnitude than any for which evidence has been found. (However, this analysis addressed only CO2 produced by the magma itself, not from interactions with carbon bearing sediments, as later proposed.)
* A reduction in organic activity would extract 12C more slowly from the environment and leave more of it to be incorporated into sediments, thus reducing the 13C/12C ratio. Biochemical processes preferentially use the lighter isotopes since chemical reactions are ultimately driven by electromagnetic forces between atoms and lighter isotopes respond more quickly to these forces, but a study of a smaller drop of 0.3 to 0.4% in 13C/12C (δ13C2 −3 to −4 ‰) at the Paleocene-Eocene Thermal Maximum (PETM) concluded that even transferring all the organic carbon (in organisms, soils, and dissolved in the ocean) into sediments would be insufficient: even such a large burial of material rich in 12C would not have produced the 'smaller' drop in the 13C/12C ratio of the rocks around the PETM.
* Buried sedimentary organic matter has a 13C/12C ratio 2.0 to 2.5% below normal (δ13C2 −2.0 to −2.5%). Theoretically, if the sea level fell sharply, shallow marine sediments would be exposed to oxidation. But 6500–8400 gigatons (1 gigaton = 109 metric tons) of organic carbon would have to be oxidized and returned to the ocean-atmosphere system within less than a few hundred thousand years to reduce the 13C/12C ratio by 1.0%, which is not thought to be a realistic possibility. Moreover, sea levels were rising rather than falling at the time of the extinction.
* Rather than a sudden decline in sea level, intermittent periods of ocean-bottom hyperoxia and anoxia (high-oxygen and low- or zero-oxygen conditions) may have caused the 13C/12C ratio fluctuations in the Early Triassic; and global anoxia may have been responsible for the end-Permian blip. The continents of the end-Permian and early Triassic were more clustered in the tropics than they are now, and large tropical rivers would have dumped sediment into smaller, partially enclosed ocean basins at low latitudes. Such conditions favor oxic and anoxic episodes; oxic/anoxic conditions would result in a rapid release/burial, respectively, of large amounts of organic carbon, which has a low 13C/12C ratio because biochemical processes use the lighter isotopes more. That or another organic-based reason may have been responsible for both that and a late Proterozoic/Cambrian pattern of fluctuating 13C/12C ratios.

Other hypotheses include mass oceanic poisoning, releasing vast amounts of CO

2, and a long-term reorganisation of the global carbon cycle.

Prior to consideration of the inclusion of roasting carbonate sediments by volcanism, the only proposed mechanism sufficient to cause a global 1% reduction in the 13C/12C ratio was the release of methane from methane clathrates. Carbon-cycle models confirm that it would have had enough effect to produce the observed reduction. Methane clathrates, also known as methane hydrates, consist of methane molecules trapped in cages of water molecules. The methane, produced by methanogens (microscopic single-celled organisms), has a 13C/12C ratio about 6.0% below normal (δ13C −6.0%). At the right combination of pressure and temperature, it gets trapped in clathrates fairly close to the surface of permafrost and in much larger quantities at continental margins (continental shelves and the deeper seabed close to them). Oceanic methane hydrates are usually found buried in sediments where the seawater is at least 300 m (980 ft) deep. They can be found up to about 2,000 m (6,600 ft) below the sea floor, but usually only about 1,100 m (3,600 ft) below the sea floor.

The area covered by lava from the Siberian Traps eruptions is about twice as large as was originally thought, and most of the additional area was shallow sea at the time. The seabed probably contained methane hydrate deposits, and the lava caused the deposits to dissociate, releasing vast quantities of methane. A vast release of methane might cause significant global warming since methane is a very powerful greenhouse gas. Strong evidence suggests the global temperatures increased by about 6 °C (10.8 °F) near the equator and therefore by more at higher latitudes: a sharp decrease in oxygen isotope ratios (18O/16O); the extinction of *Glossopteris* flora (*Glossopteris* and plants that grew in the same areas), which needed a cold climate, with its replacement by floras typical of lower paleolatitudes.

However, the pattern of isotope shifts expected to result from a massive release of methane does not match the patterns seen throughout the early Triassic. Not only would such a cause require the release of five times as much methane as postulated for the PETM, but would it also have to be reburied at an unrealistically high rate to account for the rapid increases in the 13C/12C ratio (episodes of high positive δ13C) throughout the early Triassic before it was released again several times.

### Anoxia

See also: Anoxic event

Evidence for widespread ocean anoxia (severe deficiency of oxygen) and euxinia (presence of hydrogen sulfide) is found from the Late Permian to the Early Triassic. Throughout most of the Tethys and Panthalassic Oceans, evidence for anoxia, including fine laminations in sediments, small pyrite framboids, high uranium/thorium ratios, and biomarkers for green sulfur bacteria, appear at the extinction event. However, in some sites, including Meishan, China, and eastern Greenland, evidence for anoxia precedes the extinction. Biomarkers for green sulfur bacteria, such as isorenieratane, the diagenetic product of isorenieratene, are widely used as indicators of photic zone euxinia because green sulfur bacteria require both sunlight and hydrogen sulfide to survive. Their abundance in sediments from the P-T boundary indicates hydrogen sulfide was present even in shallow waters.

This spread of toxic, oxygen-depleted water would have devastated marine life, causing widespread die-offs. Models of ocean chemistry suggest that anoxia and euxinia were closely associated with hypercapnia (high levels of carbon dioxide). This suggests that poisoning from hydrogen sulfide, anoxia, and hypercapnia acted together as a killing mechanism. Hypercapnia best explains the selectivity of the extinction, but anoxia and euxinia probably contributed to the high mortality of the event. The persistence of anoxia through the Early Triassic may explain the slow recovery of marine life after the extinction. Models also show that anoxic events can cause catastrophic hydrogen sulfide emissions into the atmosphere (see below).

The sequence of events leading to anoxic oceans may have been triggered by carbon dioxide emissions from the eruption of the Siberian Traps. In that scenario, warming from the enhanced greenhouse effect would reduce the solubility of oxygen in seawater, causing the concentration of oxygen to decline. Increased weathering of the continents due to warming and the acceleration of the water cycle would increase the riverine flux of phosphate to the ocean. The phosphate would have supported greater primary productivity in the surface oceans. The increase in organic matter production would have caused more organic matter to sink into the deep ocean, where its respiration would further decrease oxygen concentrations. Once anoxia became established, it would have been sustained by a positive feedback loop because deep water anoxia tends to increase the recycling efficiency of phosphate, leading to even higher productivity.

### Hydrogen sulfide emissions

A severe anoxic event at the end of the Permian would have allowed sulfate-reducing bacteria to thrive, causing the production of large amounts of hydrogen sulfide in the anoxic ocean. Upwelling of this water may have released massive hydrogen sulfide emissions into the atmosphere and would poison terrestrial plants and animals and severely weaken the ozone layer, exposing much of the life that remained to fatal levels of UV radiation. Indeed, biomarker evidence for anaerobic photosynthesis by Chlorobiaceae (green sulfur bacteria) from the Late-Permian into the Early Triassic indicates that hydrogen sulfide did upwell into shallow waters because these bacteria are restricted to the photic zone and use sulfide as an electron donor.

The hypothesis has the advantage of explaining the mass extinction of plants, which would have added to the methane levels and should otherwise have thrived in an atmosphere with a high level of carbon dioxide. Fossil spores from the end-Permian further support the theory: many show deformities that could have been caused by ultraviolet radiation, which would have been more intense after hydrogen sulfide emissions weakened the ozone layer.

### Microbes[edit]

A hypothesis published in 2014 posits that a genus of anaerobic methanogenic archaea known as *Methanosarcina* was responsible for the event. Three lines of evidence suggest that these microbes acquired a new metabolic pathway via gene transfer at about that time, enabling them to efficiently metabolize acetate into methane. That would have led to their exponential reproduction, allowing them to rapidly consume vast deposits of organic carbon that had accumulated in the marine sediment. The result would have been a sharp buildup of methane and carbon dioxide in the Earth's oceans and atmosphere, in a manner that may be consistent with the 13C/12C isotopic record. Massive volcanism facilitated this process by releasing large amounts of nickel, a scarce metal which is a cofactor for enzymes involved in producing methane. On the other hand, in the canonical Meishan sections, the nickel concentration increases somewhat after the δ13C concentrations have begun to fall.

### Combination of causes

Possible causes supported by strong evidence appear to describe a sequence of catastrophes, each worse than the last: the Siberian Traps eruptions were bad enough alone, but because they occurred near coal beds and the continental shelf, they also triggered very large releases of carbon dioxide and methane. The resultant global warming may have caused perhaps the most severe anoxic event in the oceans' history: according to this theory, the oceans became so anoxic, anaerobic sulfur-reducing organisms dominated the chemistry of the oceans and caused massive emissions of toxic hydrogen sulfide.

However, there may be some weak links in this chain of events: the changes in the 13C/12C ratio expected to result from a massive release of methane do not match the patterns seen throughout the early Triassic; and the types of oceanic thermohaline circulation that may have existed at the end of the Permian are not likely to have supported deep-sea anoxia.