recursal/Fanatic-Fandom huggingface dataset:
Океанія

Океанія - віртуальна держава, побудована на принципах Парламентської республіки та принципу прямої демократії, розташована неподалік узбережжя океану що на півдні від спавну.

## Державний устрій

Океанія є правовою державою, влада в якій, рунтується на засадах Парламентської республіки та принципу прямої демократії. В країні відсутній парламент, а влада здійснюється напряму Народом шляхом законотворення. Основна владна сила зосереджена в руках Уряду, що формується Народом. Формальним поточним лідером є гравець з нікнеймом shvrma238, оскільки він обраний на посаду голови Уряду (в державі відсутня позиція президента як представника влади).

## Історія

Свій початок Океанія бере на сервері Кавуневий Майнкрафт, де була заснована в середині тодішнього третього сезону. На початку це була просто купка гравців що хотіли разом побудувати велику поселення під водою, неподалік від островів посеред океану. Першим зовнішньо-політичним контактом Океанії став візит посла Сполучених Штатів Бази. Відтоді в державі, за форму політичної організації було обрано республіку, а адміністративний устрій був федеративним; країна називалась Федеративна Республіка Океанія (скорочено ФРО).

На початку нового, четвертого сезону на сервері Кавуневий Майнкрафт, Океанія стала частиною Конституційної Федерації Баштан, разом із двома іншими королівствами: Елдією та Террасеном. В середині червня сервер зазнавав різке скорочення онлайну. На фоні цих подій на сервері похитнулась політична стабільність, багато країн почало відверто агресивно відкликатись про інших. В цей час Океанія, за результатами всенародного референдуму проголосила про свій суверенітет та вийшла зі складу Баштану. Оскільки не було передбачено порядок виходу Автономій зі складу Баштану, Океанія проголосила що відтепер жодні правові акти Баштану нечинні на її території, а єдиним вищим законом в ній є її Конституція.

Після занепаду серверу Кавуневий Майнкрафт, гравці Океанії перейшли на сервер OMHMS, але як тільки стало відомо про повернення Кавуневого в обличчі Гарбузевого Майнкрафту, було прийнято рішення знову перейти на новий сервер.

## Правова інформація

Посилання на Конституцію Океанії та перелік чинних законів.
Dreams of Utopia Wiki

**Welcome to the Dreams of Utopia Wiki!**
The Dreams of Utopia Nation Roleplay Server

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## Important articles

https://dreams-of-utopia.fandom.com/wiki/The\_Confederate\_States\_of\_Americahttps://dreams-of-utopia.fandom.com/wiki/Grunder\_Industrieshttps://dreams-of-utopia.fandom.com/wiki/Realm\_of\_the\_Red\_Dragon

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M.E.G.Dark

`**成立政府，更为人道。**`

描述
--

**M.E.G.Dark**，又被称作**“主要探险者集团集群Dark分部”**。该组织由意外进入集群Dark的M.E.G.或M.E.G.CN成员共同成立，目前已正式成为集群Dark的军事政府。**M.E.G.Dark**组织对大多流浪者都极其友好，通常都会予以流浪者民事援助或庇护，并欢迎他们的加入；但不幸的是，该组织目前因观念，领土以及资源归属等原因正与其他一些组织处于交战状态。

**M.E.G.Dark**的组织成员收集编写了大量集群Dark相关文档，并将其整理归类，放置于此公共数据库，以更好的帮助流浪者脱离危险或提供指引。若有条件，流浪者可以寻找最近的**M.E.G.Dark**据点，前哨站，基地，营地或殖民地寻求帮助或庇护。**M.E.G.Dark**在整个集群Dark的分布较散乱，然而不断兼并小型组织仍然壮大了该组织的规模。

等级
--

**M.E.G.Dark**组织拥有着精细的等级划分，每名成员都有着各自的必须履行的职责，例如研究员，安全员；该种措施大大强化了**M.E.G.Dark**的组织实力，并保证了组织运作的井然有序。并且，据该组织的高级官员透露，**M.E.G.Dark**组织会定期调动管理层人员，以保持清廉的管理队伍。
Level Dark -399

我进入了玻璃门，感到非常诡异

整个楼层充斥着恶臭

腐败不堪，面目全非

我开启了柜子，里面不是杏仁水]]，取而代之的是液态痛苦

不晓得发生了啥事，整个Level Dark -399只剩了废墟

如同掉入了不幸的前厅，一切都残破不堪

底下都是一些诡异的尸体以及腐败之水

我惊呆了，尽管以前去过腐败之地

我试着拍下该层的图像，却只能拍下诡异的图片

以前住在Level Dark -399生活也是可行的，更何况与前室中的朋友视讯

我想联络M.E.G.Dark

但手机根本没讯号

我可能命中注定要被困在这

我的鞋子早已被腐败之水腐蚀了

我不想死，我还要告知所有人

我只能继续深入，就算前方存在着未知的危险

突然，我看到了一本流浪者的日记

可惜，字非常的模胡

我真心希望能得到关于这腐败之层的信息

果然，里面真有些什麽

那是一张纸条

上面的叙述很短，但也够了

纸条上写：其实这里编号原本应该是”Level Dark -399”，但却没人相信我，他们依然认为此层是Level Dark 399的副本，於是此层就被命名為”Level Dark 399-B”，但我说过了，此层一定和Level Dark 399.1相连，某天，有人开启了一扇连接腐败之地的门，最后变的腐败不堪……

纸条何时被写出来的日期更是令人感到诡异，那个日期是：

1966/6/6

Level Dark -399的组织为何隐瞒这麽久！他们绝对不可能不知道！

我继续深入

发现了一个房间

但却也变的腐败不堪

唉，这不危险才怪，同时也令我感到害怕及诡异

刹那间，手机突然跳出了讯息

我当下觉得莫名其妙

于是我便回复

“Hello？”

“你收到了啊？走向那扇玻璃门，你即可逃离。”

身后突然出现了一道亮光，我转过头来

看来这讯息绝不是那么简单的东西

我开启了这扇玻璃门

我打开了

我进入了它，回到了水淹商城

我上传了档案，编号为”Level Dark -399”，希望不会被删除

一切都只能靠运气

作者:Awe‎

图像来源:Regal Elmwood Center由Awe修改

附注:无
主页

集群Dark

集群Dark是后室中的一个超集群，然而，集群Dark虽然也存在后室中集群I，集群ZH中的大部分物资与实体，但此集群更拥有独特的一面；异于其它集群的是，集群Dark很难与集群I，集群ZH建立来往。若你不小心来到这里，那么祝你好运吧，并尽量在被实体发现前逃离到安全的地方或人类前哨。

此维基是后室分站，目前站内有 2 名活跃的编辑者，他们维护着 25 份文档（共 246 份资料） 和 38 份多媒体文件。

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Технократична Республіка Кайнокс

Bez\_darnost- (Прем'єр міністр)

```
Нині Yaxie
```

Ну що ж, вітаю в Технократичній республіці Кайнокс, вона виникла в результаті самозіслання Onii\_Chan228 на околицю мапи, де він буде будувати маленький рай для всіх потребуючих. Ця республіка є симбіозом старого концепту "Технократичної Республіки Ґенсокьо", та "Королівства Кайнокс".

## Історія:

В минулому Onii\_Chan228 жив у Імперії Тохо-Комі, і він тоді був відомий як людина, що збудувала місто за 5 днів на острові Хейватосейко, відповідно і місто мало таку ж саму назву, і все би нічого, але сталося страшне, бог жадібності, покровитель всіх жидів, демон Орест Боруква забанив двох імператорів Тохо-Комі, за те що ті дали на горіхи негіднику, що підірвав їхню залізницю на тоді ще не колонізованому острові Хейватосейко, але за них заплатили викуп 200грн, і вони повернулися на сервер, після бюрократичної тяганини виявилося, що острів належить імперії Тохо-Комі, і мені виділили цей острів під старе місто, і в результаті я збудував там маленьке містечко, але жидівський демон Орест Боруква продовжував свої підступні дії, і своєю жадібністю він розігнав майже всіх жителів того світу залишив самих лише рагулів. Мені теж прийшлося тікати, і в результаті, я опинився в цьому новому світі, як потім виявилося, мої побратими теж десь у цьому світі, але де?Арку з КК і арку з вікінгами ми опустимо з етичних причин, можу лише заявити,що в якості компенсації кавуни ТРК для СШБ тепер абсолютно безкоштовні, в майбутньому цей список доповнять тростина, бамбук, і мед.

## Державний устрій:

Країною керує голова партії, члени партії можуть лише висувати ідеї, пропозиції, скарги, і голосувати, відповідно до настроїв партії голова партії буде приймати рішення.

* Канал (тиць)
* Конституція (тиць)

## Політика:

ТРК проводить політику невтручання та самозабеспечення, до всіх країн ставиться нейтрально, виключенням є лише КК, жителям КК забороняється вхід на територію ТКР на термін 6 місяців,з етично-політичних і моральних причин, котрі я відмовляюся називати для політичної стабільності в регіоні, і для того щоб не погіршити нинішню ситуацію.

## Релігія :

Релігія: Тохо-Комівське сінто, в цьому відгалуджені сінто Юка Казамі головна богиня
悲魇

描述
--

被证实为悲魇的第一张照片。

悲魇因其外观与行为，被认定为是与笑魇对立的友善实体，数据还表明，悲魇拥有略微低于人类的智力。但是笑魇发动的种族战争使得悲魇的数量大规模减少，因此原因，目前悲魇十分罕见。悲魇的外观类似于笑魇：同样也为发光脸，然而，悲魇却是皱眉脸。因而，流浪者可以很轻松的分辨出眼前发光脸的种族。

悲魇的部分特点同样也类似于笑魇：悲魇通常只能够安全地出现在暗或者较暗处，例如黑暗的房间或走廊结构；然而，悲魇有时候也可能出现在禁用颜色的地方，也就是说，悲魇能够在完全黑白的楼层里自由活动；而若某悲魇出现在较强的光线底下，那么该悲魇个体极有可能会在十几秒内被分解掉。

尽管光线对悲魇有极大的威胁，但是异于笑魇，悲魇对摧毁光源没有任何兴趣；同样的，悲魇也对狩猎没有任何兴趣，除非被激怒，悲魇不会主动攻击流浪者或其他实体，然而，这种情况较为罕见；至今为止，仍然未观察到悲魇使用语言或文字与外界交流，但是，悲魇似乎能够理解其他实体或流浪者的语言。

悲魇虽然可能会被被激怒，但是种族战争的战败使得悲魇很害怕外界事物，因此悲魇才会结伴流浪，并且遇见其他实体或流浪者都不敢轻易靠近，直到确认该流浪者或实体对其无威胁后才敢靠近。通常情况下，悲魇会给确认友好的流浪者带来物资，还有少数报告中指出，悲魇会将该流浪者带到人类据点或者楼层出口。

悲魇和笑魇被认为是绝对敌对的，无论是位于危险楼层中的敌对笑魇，还是位于宜居楼层中的友好笑魇，悲魇都会成群结队对其发起攻击。悲魇若是被笑魇所击败，就会迅速在原地爆炸，这些爆炸虽然威力较小，但其发出的光线往往会对周围的笑魇造成重创。而对于其他危险实体，如血色狂欢，悲魇则很少理会。

M.E.G.Dark的科学家认为，悲魇和笑魇同样都是由某种未知的光学物质构成的，而外来的光线却会干扰并摧毁其内部结构，导致其个体被迅速分解。因此，悲魇和笑魇才会如此厌恶光照。还有部分论文中表示，悲魇和笑魇可能存在身体，只是无法被肉眼所观察，尽管这些论文未经证实。

发现
--

相比笑魇，悲魇的发现时间较晚。然而，在M.E.G.Dark的档案员整理了笑魇的早期目击报告后发现，其中部分报告所描述的发光脸几乎与笑魇相反，并与悲魇极其相近，该种迹象表明，悲魇极有可能同于笑魇，很早就被后室所孕育出现，但是，经过观察，大多数悲魇都只会活动于集群ZH和集群Dark，原因未知。

**> 查看著作信息**

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作者: XiaoLu408；
附注: 祝您阅读愉快！
Emojus

"I can't believe you have done this to me, treated me like an animal, and you made me this demon. I hate you, and now you will perish" Emojus after finding and about to kill his captor

He was captured at a young age by GenCorp and experimented on beyond recognition, he was treated like an animal and finally snapped and escaped while killing anyone who stood in his way for turning him into this monster, He is a broken man who feels no empathy for anyone in GenCorp.
Level Dark 2

**生存难度: Class 3**
»⠀不安全
»⠀未勘探完全
»⠀实体绝迹?

描述
--

Level Dark 2是后室Dark集群的第3层，也被称作`“2号公路”`。

黑暗向前无限延伸。

Level Dark 2的主体外观表现为无限延伸的公路，并且，该楼层因为未知原因总是处于夜晚状态中。Level Dark 2中的公路铺上了白色/黄色的油漆，其公路材质不明，而且公路坚不可摧 —— 目前没有任何已知方法摧毁/破坏公路。Level Dark 2的道路周围布满了路灯，站在路灯下的流浪者会感到不安，还有报告表明，路灯会加快附近的时间流逝，尽管这未经证实。

Level Dark 2的周围场景无法进入，其中小部分进入者在晕厥后回到了楼层的起点；大部分进入者因未知原因失去了联络(可能是死亡了)。Level Dark 2的人行道上可能会罕见地出现休息站/公交车站，里面通常会摆放一些英文报纸，其内容主要是关于被称为“2号公路”上的离奇命案(但经调查，部分命案上提到了一种被称作“Dark Shadows”的实体；且上面的部分图片中的受害者似乎是失去联络的流浪者)。

每个流浪者的Level Dark 2的外貌似乎都各不相同，但每个起点背后的道路都被封锁了。Level Dark 2的公路有很多条分支，但经记录，似乎最终都会汇聚在一起。

该楼层受类似于Level 0中的孤立效应影响，无法遇见其他流浪者。Level Dark 2中没有任何资源，且所有进入者带入的资源都会随机减少一定量。因而很少有流浪者能走到红色告示牌下，并卡出该楼层，到达Level Dark 3；除此之外，没有任何方法离开该楼层。

派至Level Dark 2的大多数探员最终都失踪了，但M.E.G.Dark目前仍然没有放弃对Level Dark 2的调查。

实体
--

> 该楼层中没有任何实体。

> 我们错了，完全错了...

`M.E.G.Dark录像带(发现于Level Dark 3)`

视频中的探员不停向前跑，还不时回头，似乎有什么在追着他，一直持续了2分钟。

探员跑到了一处休息站，再次转头，什么也没有。

“终于甩掉了...”

录像中突然闪出一道黑影，探员躲了过去。

*录像中断。*

*录像恢复。*

探员喘着气，听到脚步声，立马停止了喘气。

探员给手枪上了膛。

突然，探员所在的房间的门被缓缓打开。

一道黑影迅速靠近，探员起身扣下几次扳机都没有击中。

录像机掉到地上，屏幕被一滴血遮住了。

最后一声枪响。

*录像结束*

目前尚不清楚为何这则录像会出现在Level Dark 3，可能是那名探员杀死了实体逃离这个楼层；但更有可能是实体对我们的挑衅，至此，我们将停止对Level Dark 2的调查，不能再有人死了...

> 查看著作信息

> 收起著作信息

作者: 晓雾；
图像来源: 由AI生成。
Zai

"GenCorp has taken everything from me, so I will take everything from them." - Zai's journal day 50

Zai is the vice-leader of The Hero Squad, right below the leader Akon. Zai at first worked for GenCorp until The Hero Squad revealed the horror of GenCorp and then he decided to quit and destroy the GenCorp facility he worked at. A few years later, Zai had awakened their full potential.
Cosmic Spider

"He was a purple spider-like creature with the eyes of a million galaxies, I saw him in the sky, staring at me like he was, observing my every move, studying me, or maybe... guiding me, he was of an immeasurable size from my standpoint, he looked infinite." - Akon describing his encounter to James

Cosmic spider is The Creator's right hand man and guides heroes to their destinies so they can unlock their full potential but XIU$H$J always tries to absorb the heroes; thankfully cosmic spider is one of the only beings who can stop it.
Level Dark 922

这是我来到后室的第922天，也是我落魄流浪的第922天。

我站起身，拿起木浆，继续划动木舟。木舟缓缓向前行驶，但汪洋大海中，木舟就如同蝼蚁般渺小。海上的大雾从我受困到现在，一直都没有消散，整个楼层没有丝毫光芒，如同我前方的人生一样。可以说，我完全是凭直觉在前行 —— 我甚至不知道我是不是在兜圈子。

又是几小时，我还是再重复的大海上，我意识到这是徒劳无功；我在迷途中行驶了许久，但我……甚至不知道，我究竟在为什么而前行……我倒在地上，随之倒在地上的还有背包，里面的东西都撒了出来。我翻找背包，所有物资都没了，但在这里，所有生理需求都被延缓了；突然，一张泛黄的照片吸引住了我，一滴眼泪掉在了上面：

当时，一场地震带走了我的家庭，除了在外出差的我和老家里的父母。得到信息后，我立马乘高铁回到了家，家已然成为了一片废墟。我不知道该怎么办，没有目的翻着废墟，最后，我翻出了一张照片 —— 相框的玻璃已经被石头压碎，上面布满了灰尘。我伸手擦拭照片，那是……我的……全家福……唯一使我感到安慰的是，石头没有把它刺穿。

祸不单行，公司的破产害我一无所有。我变得无向所从，人生剩余的道路被迷雾阻挡。我走到海边，海上正大雾弥漫，我正要走进去。幸亏，巡警发现不对拉住了我。微光透过薄雾，我决定重新开始这一切。我找到当初在我光辉时期来找我帮忙的所有人，结果他们听说了我的遭遇后，全拉黑了我；没有其他办法，我只能借贷款。终于，2年的努力，我创业成功了，然而，路还不是平坦的。

又是一个噩耗，母亲打来电话，父亲重病，让我尽快赶回去，要不然可能以后再也见不到他了。我急匆匆的跑出办公楼，豆粒大的雨打在我身上，我没有带伞。突然，我被石头绊倒了。再次睁开眼，一切都不见了，眼前只有黄色，很多黄色……

万幸，意外卡出Level 0后，M.E.G.CN.的人找到了我。他们告诉我，我卡入了后室，而这里是Level 1，这里几乎不遵守任何物理法则。我是一个相信科学的人，直到几天后，我才勉强接受了事实。

这段时间我都住在Level 1，直到那天，我本来就和往常一样，正在寻找物资。可突然，灯灭了，一只发着亮光的笑脸向我逼近。我不知所措，只能盲目逃跑，想逃回前哨站，但我毫无防备的卡入了墙内，离开了Level 1。但往好处想，我至少暂时摆脱了危险。

在离开了让我险些失去性命的Level 2，Level 3后，我在Level 4短暂休息了一段时间；后来，我不幸来到了Level 8，但我居然成功逃离了，在这里，我第一次杀死了实体，这比我想象的要容易多了。接下来的两年内，我又去了无数个楼层，有的安全，我就短暂休息了会，并补充资源；有些危险，但万幸，我都通过了。当时流浪者间流传着一个传说 —— Level ZH 399能够返回现实。

直到我真正来到Level ZH 399，才发现，这根本就是一个谎言，或者说，这个结局早已消逝。我心灰意冷，坐在一盏霓虹灯下，而它根本没带来丝毫放松。我想到了那个假的家园，我决定了，在那里结束可悲的一生。

然而，我没有到那里。我来到了一只木舟上，海面显得格外平静，就像当年我看到的海一样，大雾弥漫。我尝试了卡出等好几种方法，但都无法离开。绝望中，我掏出匕首想要自刎，然而，可能是因为楼层的特殊机制，刀没能对我造成任何伤害。我决定，到达楼层的终点，我相信那一定有着出口。

回到现在，已经过去了将近一年了，我还是在大海上，没有任何人，或实体。我在这一年中，早就迷失了方向，我知道这只是徒劳。再次擦拭那张泛黄的全家福，突然，上面反射过光线，我抬起头，迷雾仿佛就要散去，一切都逐渐清晰了，我知道，是有光线照来了……

万物皆有裂缝，那是光照进来的地方……而若我们不放弃，终会找到那缝隙。

> 打开著作信息

> 关闭著作信息

作者: 晓雾
Level Dark -1

无边无际的美丽湖泊。 ~

LEVEL DARK
**-1**

生存难度

**Class 1**

**逃脱指数: 1/5**

易于逃脱

**环境指数: 1/5**

极少环境危害

**实体指数: 0/5**

无有害实体

描述
--

* **Level Dark -1**也被称作“人造湖泊”，该楼层表现为一处被山脉环绕的人造湖泊，据记录，该楼层中还存在着与前室高度相似的昼夜变化以及自然现象。最初发现该楼层时，M.E.G.Dark当局将它定义为天然湖泊，后来勘探队发现湖泊的对岸存在大量未知材质机械，这些机械通过许多条未知材质水管连接着山后。据勘探队初步推测，这些机械可能是用于蓄水的，而这些水管可能是用于连接水源的。后来这些推测得到了证实，但是这些机械蓄水的情况很罕见并且发生的时间完全随机。不幸的是，因为山脉过于高耸陡峭，导致山脉以及山后区域的勘探难度过大，直到目前为止，仍然不清楚蓄水机械所连接水源的具体位置。而在此之后，M.E.G.Dark当局也因此重新定义了该楼层，并将该楼层改名为“人造湖泊”。由于该楼层的特殊性质，现将其分为以下区域进行介绍：

### 沿岸区域

* 沿岸区域的外观表现为一片沿水沙滩，该区域是**Level Dark -1**中最适合生存的地点。沿岸区域始终都被一种奇怪的薄雾所笼罩着，这种薄雾的颜色十分浅淡，以至于M.E.G.Dark探员几乎无法用拍摄设备对这种薄雾进行记录。出于某种未知原因，吸入这种雾气会给流浪者带来异常的轻松感，并慢慢地缓解流浪者进入后室以来遭受的所有心理伤害。沿岸区域中还零零散散地分布着一些对于流浪者而言极其重要的生存物资，这是该楼层已知唯一的物资来源，但值得注意的是，该楼层中所有的杏仁水和腰果水都被换成了同样的包装，如果需要饮用，必须先仔细辨认。

### 湖泊区域

* 湖泊区域相比起宜居的沿岸区域，勘探难度呈直线上升，因此原因，直到目前为止，有关该区域的信息仍尚未收集完全。湖泊区域无论是其内部还是其表面都显得十分空荡，流浪者能看见的只有无数长相奇异的岩石。该区域直到现在，也没有任何被证实的原生水生生物目击报告。过长时间探索该区域的行为是不被推荐的，因为这可能会导致流浪者氧气耗尽或精疲力竭，导致其被困湖泊。湖泊区域的深度是未知的，但M.E.G.Dark当局并不建议流浪者深入探索湖泊区域，不仅是因为湖泊区域勘探难度过大，更是因为深入湖泊区域有可能会导致流浪者来到虚空。流浪者如果要饮用从湖泊区域中提取的水，最好先将这些水进行淡化处理，因为这些水的内部结构与前室中海水的内部结构极其相似。

### 山脉区域

* 山脉区域的范围包括**Level Dark -1**中的山脉及山后，但该区域仅是一个假设。如上所述，流浪者想要到达该区域十分困难，到目前为止，没有任何被证实的山脉翻越记录。所有电子设备在山脉区域似乎都不可用，因为M.E.G.Dark当局曾经四次试图使用无人机勘探山脉区域，然而这些尝试都没有成功。据M.E.G.Dark当局推断，山脉区域很有可能还储存着大量水源，并以此供应蓄水。

现象
--

湖泊似乎有些不对劲。 ~
### 涨潮

* 涨潮是指蓄水机械开始异常运作时，该现象极其罕见但是十分危险。涨潮时期的湖泊区域将变得汹涌澎湃，并且会不断升高水位，以此扩张湖泊区域的范围，如果流浪者不幸被卷入了涨潮时期的湖泊区域，那么他的身体将会在数秒后被湖泊巨大的拉扯力撕碎。万幸的是，M.E.G.Dark当局在发现存在涨潮现象后，联合其他组织修建了人防工程。现在，流浪者只要不在涨潮时期进入湖泊区域就不会危及生命安全。涨潮现象发生时该楼层湖泊区域生存难度将会发生巨大变化：

LEVEL DARK
**-1**

生存难度

**Class 3**

**逃脱指数: 5/5**

无法逃脱

**环境指数: 5/5**

死区

**实体指数: 0/5**

无有害实体

### 退潮

* 涨潮现象结束后，湖泊区域就会开始缓慢恢复水位，M.E.G.Dark当局把这种现象称为退潮。经推断，这些退回的水可能是被排往了原处。异于涨潮现象，退潮往往只需要几小时就可以完成，但是M.E.G.Dark当局仍然明令禁止流浪者在退潮时期进入湖泊区域，因为湖泊区域无论处于涨潮时期还是退潮时期，都十分危险。退潮现象结束后该楼层湖泊区域生存难度将恢复如初。

实体
--

* 截止目前为止，**Level Dark -1**中仍然没有发现任何实体。

## 据点与前哨站

### M.E.G.Dark湖泊状况监测局

* 拥有33名常驻成员；
* 会对涨潮现象发布预警；
* 友好，且开放贸易；
* 拥有小规模武装力量；
* 主要目的是探索**Level Dark -1**。

### 沿岸居民

* 拥有64名成员；
* 愿意收留流浪者；
* 友好，且开放贸易；
* 乐于帮助流浪者。

## 入口与出口

### 入口

* 跳入Level Dark 74中的任意湖泊，流浪者都会被传送到该楼层。
* 找到任何与**Level Dark -1**相关的邀请函并卡入它都能带流浪者到达该楼层。
* 在长途跋涉中的停车场内做梦有大概率来到该楼层。

### 出口

* 潜入湖泊深处的流浪者将会抵达虚空。
* 只要流浪者在沿岸区域走得够远，就有极低的概率会到达Level Dark 399。
* 被卷入涨潮时期的湖泊有极小的概率会到达Level Dark -3。
* 卡入沿岸区域的地面会使流浪者来到Level Dark -2。

脚注
--

1. ↑ **Level Dark -1**中的一个标准日为22小时，其中白天有10小时，夜晚有12小时。
2. ↑ 导致涨潮与退潮现象持续时间差异的原因未知。

**> 查看作者信息**

**< 隐藏作者信息**

作者：XiaoLu408
图像：来源自互联网
Macrophage cell culture

Macrophages are able to initiate a particular type of inflammatory response *via* the release of a certain pattern of cytokines. They play a major role as immunoregulatory cells. Creative Biolabs has built a highly experienced team of scientists and quality staff that have a long history in macrophage isolation and culture. Human monocyte, human alveolar, murine peritoneal cavity, murine bone marrow, murine lung, and murine adipose tissues are available for macrophage isolation and culture. Our seasoned scientists are pleased to share our expertise and experience with our global clients and facilitate their meaningful macrophage project development.

Creative Biolabs combines deep, industry-leading expertise with an innovative Macrophage Therapeutics Development Platform to provide high-quality human/murine macrophage isolation and culture services. These isolated macrophages can be examined in vitro for their roles in phagocytosis, cytokine and mediator production, and other biological functions. Our R&D scientific team has accumulated extensive experience in comparing profiles of macrophages between controls and patients, which allows us to effectively determine the changes of the precursor number or the phenotype, with the eventual aim to help our clients develop cell therapies. For more information, please feel free to contact us and further discuss with our scientists.
Axl antibody

**axl antibody**

# AXL

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Full Name

AXL Receptor Tyrosine Kinase

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.

(Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

(Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.
Hyper IgE Syndrome

As a senior expert in the field of IgE research, Creative Biolabs is willing to provide our customers with a wealth of Hyper IgE Syndrome (HIES) -related background information.

### Introduction of HIES

HIES is recognized as a complex immunodeficiency disease accompanied by repeated lung and skin infections with bacteria, atopic dermatitis, and extremely high serum IgE levels. The initial high IgE syndrome is characterized by a weakened inflammatory response, accompanied by *Staphylococcus aureus* skin abscess and pneumonia, followed by emphysema. Clinically, the skin manifestations of patients with HIES are similar to common atopic dermatitis. These immunological findings are usually related to bone and connective tissue abnormalities. Most patients manifest beyond immune system abnormalities, including bone and connective tissue abnormalities, such as scoliosis, osteoporosis, joint hyperextension, unique facial appearance, and deciduous teeth retention.

### Genetics

Studies have identified that most autosomal dominant HIES (AD-HIES) are closely related to the mutation of STAT3. AD-HIES patients will also exhibit abnormalities in connective tissue and bones. The multi-system abnormalities in AD-HIES patients confirm that STAT3 not only plays an important role in the immune system, but also has extensive connections with teeth, musculoskeletal, and vascular systems.
Half-Life Strategies in Supporting the Development of Protein Therapeutics

Therapeutics based on macromolecular proteins and peptides have successfully been efficiently applied to treat serious human diseases. With the development of biotechnologies, a great number of authorized protein therapeutics and drug products have been utilized in clinical research over the last few decades. However, the main issue that needs to be resolved currently for protein therapeutics is the short half-lives caused by fast degradation in serum and quick elimination during clinical application due to enzymatic degradation, renal clearance, liver metabolism, and immunogenicity.

After putting in a lot of effort over the years, researchers have discovered some effective strategies for extending the half-life of protein therapeutics and biopharmaceutical products, such as polymers in drug half-life extension. Polymer conjugation is one of the widely used and efficient techniques in drug half-life extension research, and PEGylation in drug half-life extension is an efficient method for improving pharmacokinetic properties owing to its highly hydrophilic and mostly non-toxic features.

As protein engineering technology advances, bioactive natural protein conjugation, due to its lower toxic side effects, is becoming more accepted as a competitive method to prolong the half-life of drugs. Currently, diverse technologies of bioactive natural protein conjugation are being developed for the half-life extension of drug research, including:

\* Albumin-based half-life extension

Albumin conjugation has been extensively used in a variety of protein drugs on the market.

\* Fc-Fusion-based half-life extension

The Fc-Fusion technique works well for most therapeutic protein modifications.

\* Transferrin fusion-based half-life extension

Transferrin fusion, a novel method to realize half-life extension, has potential clinical application.

In addition, lack of efficacy continues to be a major driver of drug candidate attrition. The drug half-life assay, as the first essential step in drug development to determine the half-life of drugs, is an essential strategy for selecting suitable drug candidates for clinical trials. In this case, many CRO companies are working hard to provide a variety of assays for improving the evaluation of drug candidates, as did Creative Biolabs. Having updated their advanced and comprehensive half-life assay platform, they are confident in providing high-quality half-life assay services to global customers, including but not limited to drug half-life *in vitro* and *in vivo* detection services.

Creative Biolabs, as a global CRO company focusing on drug development for years, has accumulated extensive experience in half-life extension and won a prominent reputation from customers all over the world. Scientists at Creative Biolabs continue to improve its half-life extension technologies in order to provide customers with the best drug half-life extension services, contributing to accelerating drug development.
The Current Progress in NASH Diagnosis

Biomarkers are diagnostic tools that can predict disease progression in an individual patient and/or identify the patients who are more likely to respond to a particular treatment. Therefore, biomarkers have the potential to accurately inform treatment decisions and improve disease outcomes. It is well known that when NASH progresses to the final stage, necrotic inflammatory changes and serum transaminase levels gradually decrease. Currently available biomarkers are not superior to serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in identifying NASH patients with poor sensitivity (< 50%).

The ideal biomarkers should be easy to measure, repeatable, inexpensive, and accurate, especially for distinguishing NASH from simple steatosis and predicting the risk of disease progression. Such biomarkers would not only aid clinicians in the identification of patients with NASH but also allow for noninvasive and frequent monitoring of disease progression and response to therapy. These biomarkers include indicators of hepatocyte apoptosis, oxidative stress, and inflammation, as well as predictive models based on clinical variables. Furthermore, the biogenesis and composition of extracellular vesicles (EVs) have suggested that they might be also potential biomarkers for NASH diagnosis.

Emerging biomarker research in NAFLD and NASH will help pave the way for alternative noninvasive methodologies and potential therapies. Creative Biolabs provides non-invasive and reliable biomarkers development services for liver injury during NASH development. These biomarkers include but not limited to:

* Markers of Apoptosis
* Markers of Inflammation

* Markers of Oxidative Stress
* Markers of Exovesicles (EVs)
Phytosomes

At present, expensive research in novel drug delivery systems is going on to improve the therapeutic efficacy of the existing natural molecules. Extensive research in the field of herbal drug delivery systems as a means of improving the therapeutic indices of drugs is inevitable and thus phytosomes came into being. With integrated bioactive molecule development and engineering techniques, Creative Biolabs offers a comprehensive set of vesicular system that provides either individual service modules or final customized products for our valued clients. At present, we are capable to supply various services about phytosomes. We also provide solutions tailored to our customers’ unique specifications.

Natural products have been frequently used as sources of clinical useful pharmaceuticals. Hydrophilic constituents could be complexed with clinically useful nutrients such as phospholipids to convert them into lipid soluble complexes. This kind of complexes can be used to prepare liposome-like vesicles called phytosomes. In phytosomes, the complexation of phospholipids and water soluble active plant components involve chemical bond formation and therefore more stable. However in liposomes no chemical bond is formed and phosphatidylcholine molecules simply surround the water-soluble components. The phytosomes substantially improve the bioavailability of these hydrophilic active components. Phytosomes can easily cross the lipid membranes and are reported to increase the bioavailability of poorly lipid soluble plant based drugs by increasing the absorption in gastrointestinal tract.
C-Maf and γδ T Cell

### Short Introduction of c-Maf

c-Maf is a member of the basic leucine zipper transcription factors belonging to the AP-1 family. It has been shown that c-Maf is overexpressed in multiple myeloma (MM), enhancing tumor-stroma interactions. In the monocyte/macrophage lineage, c-Maf is essential for macrophage self-renewal.

### c-Maf and γδ T Cell

Maf deficiency caused an absolute lineage block at the immature CD24+CD45RBlo γδ thymocyte stage. c-Maf enforced Tγδ17 cell identity by promoting chromatin accessibility and expression of key type 17 program genes, notably *Rorc* and *Blk*, while antagonizing the transcription factor TCF1, which promotes interferon-γ-producing γδ T cells (Tγδ1 cells). Furthermore, γδ T cell antigen receptor (γδ TCR) signal strength tuned c-Maf expression. c-Maf is considered a universal regulator of Tγδ17 cell differentiation and maintenance.

### c-Maf in T Cells

c-Maf expression and activity are regulated at transcriptional, post-transcriptional, as well as post-translational levels. Transcription factors and RNA-mediated silencing control the amount of Maf transcripts, while phosphorylation will modify the activity, subcellular localization, and half-life of the protein.

In T cells, antigenic stimuli that modulate the stability of the c-Maf encoding mRNAs and/or the c-Maf protein may also induce the expression of transcriptional activators, or may cooperate with independent transcriptional stimuli, such as cytokine-driven STAT factors to induce c-Maf transcription. The selective use of those pathways by different stimuli and in distinct cell populations provides the potential for tailoring c-Maf expression to different circumstances.
Cataliesw Wiki

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IgY production

IgY purification by means of protein A (Staphylococcus aureus) or protein G (Streptococcus species, strains C and G) affinity columns is not possible, since these proteins have no binding site for IgY. The choice of a suitable IgY purification method is influenced by the scale of purification (laboratory or industrial), cost-effectiveness, technology, and impact on the environment (waste management).

Precipitation methods: Involving ammonium or sodium sulfate, polyethyleneglycol (PEG), caprylic acid, and carrageenin.

Chromatographic methods: Affinity chromatography, ion-exchange chromatography, hydrophobic interaction chromatography, thiophylic interaction chromatography, and gel-filtration chromatography.

Ultrafiltration: The purity of an IgY preparation can be increased by combination; for example, PEG precipitation can be combined with affinity chromatography.
Level Dark 999

⚘⚘**生存难度: Class 宜居**» 安全» 稳定» 无有害实体

Level Dark 999。

描述
--

Level Dark 999是后室Dark集群的第1000层 —— 也是集群Dark的最后一层。因其绝对的宜居性，此楼层在流浪者之间广为流传。

Level Dark 999的外观类似于毁灭前的Level 6.1，然而，Level Dark 999却并非人造，而是后室自身的产物。Level Dark 999相比Level 6.1，更加的未来化，并且遍布霓虹灯。该楼层中，食物是最不需要担心缺少的，每一家零食室/小吃店内都摆满了不会过期的食物，其中包括杏仁水。Level Dark 999中的所有房间似乎都规律排序。

Level Dark 999中的收银台上可能会罕见的出现一张任务清单 —— 每个流浪者也只能拥有一张，如果流浪者尝试换一张任务清单，或拾取多张任务清单，就会被看管小吃店的无面人驱赶出楼层，且无法再次返回。只要按要求完成任务，下一次入睡后，流浪者就会在前室的睡觉的地方醒来。有的任务很简单，如连续喝几瓶杏仁水；有的任务则很困难；如在无防护措施下近身杀死几只血色狂欢感染者。

该楼层中的霓虹灯也显异常，所有处于霓虹灯照射范围的流浪者都会感到舒适、放松；并且，除了会治愈流浪者心理上的疼痛，霓虹灯还可以治愈流浪者生理上的疼痛。然而，霓虹灯一旦损坏，即使被人工修好，也会失去所有作用，原因未知。

Level Dark 999是极少数能够返回前室的楼层之一。

实体
--

所有敌对实体都会消失在该楼层，原因未知；但该楼层的各个小吃店内都有无面人员工，且均为友好实体。

## 殖民地与前哨站

### M.E.G.Dark Alpha据点

该据点成立于2022年，并于2023年扩大了范围及成员数量，成为了Level Dark 999中最受欢迎的据点之一；该据点友好且开放贸易，拥有56名成员。

### 后室红十字会

* 拥有大量医疗资源；
* 主动提供医疗服务；
* 有11名成员；
* 长期与M.E.G.Dark合作。
CAR-T in vivo assay

Taking advantage of the CellRapeutics™ platform, Creative Biolabs provides customized, standardized, reliable and high-quality CAR-T preclinical in vivo assay services for clients across the world. Our one-stop service covers all aspects from cell biology to animal tests with a special emphasis on the construction of tumor xenograft models. Various robust animal models and assay systems have been used in-house to greatly facilitate CAR-T development and evaluation projects with far less time and budget.

CAR-T cells are autologous or allogeneic T cells targeting specifically to antigens or markers expressed by tumor cells. In vivo assessment is of pivotal importance for investigating the factors that may affect the efficacy of CAR-T cells. The approaches of delivering CAR genes into T cells, the in vitro culture conditions, the constituents of the CAR construct, and the types of host T cells all have great impacts on CAR-T efficacy and safety. Data from in vivo assays is valuable for CAR-T optimization. CAR-T cells capable of recognizing various tumor-associated antigens (TAAs) have been tested in many clinical trials and received plenty of encouraging results. However, several potential risks such as cytokine storm have also been posed for CAR-T cell clinical application. Therefore, preclinical in vivo assessment of the efficacy and safety of CAR-T cells are essential for dosage determination and risk managements for this promising immunotherapy against cancers.

Learn more: CAR-T in vivo assay
Agonistic Antibody Therapy

Manipulation of the immune response with either antagonistic antibodies of immune-repressor molecules or agonistic antibodies of immune-activating receptors has found great potential in the application for immune disease, cancer, and infectious disease therapy. While the antagonistic antibodies dominate the immune-checkpoint antibody drug discovery field, agonistic antibodies represent promising agents and various studies have reported antibody development targeting costimulatory molecules, such as CD27, CD40, OX40, 4‑1BB, GITR, ICOS, and CD28. Here, Creative Biolabs gives a brief introduction to the therapeutic application of agonistic antibodies in different diseases.

**Agonistic Antibody Therapy in Cancer**

Cancers can co-opt the immune-checkpoint pathways to evade the immune system. However, the discovery of the fact that therapeutic antibodies that block these receptors can take the brakes off the anti-tumor immune response and stimulate antitumor immunity, contributing to the development of immune-checkpoint antibodies. Antagonistic antibodies targeting coinhibitory receptors, such as CTLA-4 and PD-1, have been approved as monotherapies for advanced melanoma, lung cancer, and evaluated for the treatment of other types of human cancers.

Besides targeting the inhibitory pathways, agonist antibodies directed against the immunostimulatory receptors can also stimulate antitumor immunity and are emerging as a promising area of clinical development for cancer immunotherapies. For instance, Vonderheide et al. (2013) reviewed the use of agonistic CD40 monoclonal antibodies (mAbs) for cancer therapy, which has shown highly promising results without disabling toxicity. This CD40 mAb can activate antigen-presenting cells (APCs), promote antitumor T-cell responses, and foster cytotoxic myeloid cells with the potential to control cancer in the absence of T-cell immunity.
Potential Treatment Strategies for CNS Diseases Require More Research

For several decades, CNS diseases have caused serious burdens for individuals with these diseases in both physical and mental health. Studies show that more than 95% of CNS diseases have an unknown etiology, which presents scientists with significant obstacles on the road to the creation of efficient treatments. Parkinson's disease and Alzheimer's disease are the two most common types of conditions in the group of patients with CNS diseases, and researchers only have a few findings after years of efforts due to the complexity of the brain. However, new research conducted recently on these two types of illnesses has revealed some unexpected breakthroughs.

Recently, scientists at the Salk Institute published a paper in *Cell Stem Cell* indicating that people with Alzheimer's disease develop neurons that degenerate and experience a late-life stress process known as senescence, and these degenerating neurons will release inflammatory factors, triggering a cascade of brain inflammation that sends other brain cells out of control, which further proves that targeting senescent cells may be an effective way to slow down neuroinflammation and neurodegeneration in Alzheimer's disease. They also pointed out that some known drugs that cross the blood-brain barrier (BBB) may be used as treatment options in the future. There is no doubt that the findings give significant hope for patients with Alzheimer's disease. At the same time, it also represents another significant step forward in the treatment of Alzheimer's disease.

Meanwhile, a new potential therapy targeting Parkinson's disease is also being brought forward by scientists from the UK and Sweden, who indicated that nerve cells transformed from stem cells produced in a lab will be utilized to replace those destroyed by the disease, and they hope these can stop the spread of debilitating symptoms. Indeed, replacing degenerating dopamine cells in the midbrain of Parkinson's disease patients with transplants of dopaminergic neurons is seen as the most promising therapeutic approach. This will prevent the clinical progression of the disease due to its substantial loss of midbrain dopamine neurons in the substantia nigra pars compacta and decreased innervation to the striatum. As a result of its importance in the research of a new Parkinson's disease therapy, dopaminergic neuron differentiation has come to the attention of researchers. However, a great number of studies still need to be done for successful applications in CNS disease treatment.

As a pioneering biotech CRO dedicated to exploring neurological models for decades, Creative Biolabs has developed a comprehensive technology platform with a variety of available models, equipped with advanced facilities, experienced experts, and the latest technologies. The company is confident in its ability to provide global customers with a wide range of neuroscience services and neuroscience research tools, such as custom dopaminergic neuron differentiation model services, to accelerate their neuroscience research.
Level Dark -999.9

LEVEL DARK
**-999.9**

生存难度

**Class 5**

**逃脱指数: 4/5**

极难逃脱

**环境指数: 5/5**

死区

**实体指数: 5/5**

敌对实体侵占

**Level Dark -999.9**是Level Dark -999的亚层，是后室集群Dark中极其隐秘与诡异的一层。它在某种程度上并"不存在"。

Epic
## > 描述

Level Dark -999.9的外观与结构取决于流浪者的状态，但大多呈现为前室中的建筑。尽管如此，楼层的建筑与结构似乎会随着时间流逝逐渐转为单一类型，但尚不清楚原因。每种建筑都具有一定的差异，但它们都含有3个共同的元素。第1个是处于永夜状态，第2个是使流浪者缓慢消失直到完全融入混沌，无论是流浪者主动融入还是被该层结构强制融入，第3个是随着离楼层中心越远混沌程度会越高。但该层相比Level Dark -999来说较为稳定且突然重塑的频率 (相对) 也更少。该楼层还能消除流浪者在来到该楼层前所得到的任何疾病，无论是生理还是心理的。温度较为稳定，经常保持在 25°C (77°F) 左右。

**?**

在Level Dark -999.9中的环境可以说是异常宁静的，目前分贝值已探测到最低为约-9db，这通常会使流浪者感到恐惧。由于某种原因，在Level Dark -999.9中所有人工照明会全部失效，且来到此处的流浪者也会因某种原因导致彼此无法相遇，使对于该楼层的勘探难度大幅增加。在Level Dark -999.9中的建筑皆由组成Level Dark -999类似的材质所构成，许多性质与Level Dark -999相似。唯一与Level Dark -999不同的是，它们不具有保护机制，因此通过破坏墙壁来帮助勘探是完全可行的。Level Dark -999.9中的物资相比Level Dark -999来说十分稀少，包括但不限于核桃糕，杏仁水与前室中的食物，但它们大部分是变质的。
当流浪者在Level Dark -999.9停留过长时间时，该层将会进入缓慢融入至混沌的状态。事件发生后该层的任意结构将开始慢慢瓦解直到完全消失，在瓦解过程中还能观察到位于楼层底部的腐化之物海洋。此时与Level Dark -999性质相似的黑洞将开始大量出现并吞噬掉整个楼层，天空逐渐"故障化"，楼层与建筑开始失去稳定性并开始下坠与坍塌，底下的腐化之物开始上升，物资也不在生成。然后...

结束了

结束了

这就是"结局"

一个不存在的"结局"

一个属于你的"终点"

结束于那个扭曲之地的亚层

结束于一个未经证实的"楼层"的亚层

它的性质亿万年来是如此

也许，它在某种程度上并"不存在"

且是"不存在"中的"不存在"

它使你被遗忘于"不存在"的深处

成为"不存在"中唯一"存在"的的物质

或许，存在一种方法能够阻止它

或许和那个那个"结局"的逍逝有关

它使许多人尝试寻找一个新的"结局"

以至于过于深入探索后室集群Dark

但...寻找一个新的"结局"似乎并不是简单的事

于是有人冒险去勘探了那个扭曲之地

也正是那个传说中的"终点"所在的楼层

但那个扭曲之地似乎知道流浪者的目的

于是便将流浪者传送与此

在一个比地狱更加糟糕的地方

一个"不存在"的纬度

一个使你接受"堆栈溢出"错误的楼层

一层层的现实坍塌在你身上

它的重压给予你一种空虚的感觉

它的重压使你消失在混沌中

希望早已破灭，出口已经早已失去意义

结局已经到来

渐渐灰飞烟灭……使你成为混沌的一部分。混沌的一部分

混沌的一部分

混沌的一部分

混沌的一部分

混沌的一部分

混沌的一部分

...

插入文章

作者:Awe‎

图像来源:来源自免授权网站

附注:无

1. ↑ 这点和Level Dark -999很像。
2. ↑ 约6天。
In Vivo Model Development for Immune Checkpoint Drugs

During the drug discovery process, *in vivo* testing is critical for assessing drug efficacy. Equipped with advanced platforms and extensive experience, Creative Biolabs is always dedicated to assisting our clients with the most satisfactory *in vivo* model development services for immune checkpoint drug discovery.

### introduction to *In vivo* Model

*In vivo* testing focuses on the pharmacokinetics of the drugs in the living organism, especially drug safety, metabolism, and efficacy. Compared with *in vitro* testing, *in vivo* testing is more time-consuming and more demanding. In general, *in vivo* testing is followed by *in vitro* testing to test whether the compound can achieve the expected purpose and the potential risks that it may bring. As the key component of drug discovery, it is the only way to fully evaluate the pharmacology, pharmacokinetics, efficacy, and safety of the potential drug candidate.

In recent years, animal models have been served as the most common test subjects for preclinical pharmaceutical and biomedical research. There are a variety of animal models for drug discovery, which include but not limited to mice, rats, rabbits, dogs, cats, and non-human primates. The animal models can simulate the human system well to predict the efficacy of drugs on the human body, including the success or failure of the drug *in vivo*. In addition, the *in vivo* testing also provides relevant information on the drug's mechanism of action research.

Learn more: In Vivo Model Development for Immune Checkpoint Drugs
Probiotics for obesity

The gut microbiome has been identified as one of the most important factors in obesity pathogenesis and regulation. As a world-leading services provider, Creative Biolabs is confident in providing a full range of probiotic products and high-efficiency services for obesity treatment and prevention.

**Overview of Obesity**

Obesity or overweight is medically considered as a kind of chronic metabolic disease in which abnormal or excessive fat accumulates causing multiple adverse effects on human health. Over the past few decades, the prevalence of obesity worldwide has more than tripled since 1975, leading to current approximately one-third of the population now living with obesity. As a major public health problem, obesity is known to cause a series of human diseases and pathologies, among which cardiovascular diseases, diabetes, fatty liver, cancers are the most common types.

Creative Biolabs is proud to be one of your trusted partners in live biotherapeutics drug discovery. Our talented scientists with innovative technology platforms are always ready to provide fully custom services for the prevention and treatment of obesity and obesity-related metabolic disorders using potential probiotics. In addition to a spectrum of probiotic products, a wide range of custom analytical development and qualification services are also within our scope, which mainly include but not limited to:

Microbial Identification Services

Biological Safety Test

Potency Test

Stability Test

Antibiotic Susceptibility/Resistance Test

Learn more: probiotics for obesity
Level Dark 0

集群Dark是后室中的一个超集群，然而，集群Dark虽然也存在后室中集群I，集群ZH中的大部分物资与实体，但此集群更拥有独特的一面；异于其它集群的是，集群Dark很难与集群I，集群ZH建立来往。若你不小心来到这里，那么祝你好运吧，并尽量在被实体发现前逃离到安全的地方或人类前哨。 ~

LEVEL DARK
**0**

生存难度

**Class 1**

**逃脱指数: 2/5**

较难逃脱

**环境指数: 2/5**

较少环境危害

**实体指数: 0/5**

无有害实体

描述
--

此楼层最广为人知的照片。

经侦测，**Level Dark 0**同大多数楼层一样，也是一处非欧几里得空间。该性质使得GPS或指南针等道具在该楼层中完全失效，因此，对该楼层的探索极为困难，甚至是根本无法将**Level Dark 0**勘探完全。在该楼层滞留过久可能会引起流浪者的不安，恐惧等异常反应，因此，大多数流浪者只是将该楼层当作中转站。该楼层的主要区域外观表现为一处类圆形湖泊，此外该楼层还被分为沿岸区域，湖泊区域，人造区域以及山脉区域；但是山脉区域经过证实，楼层的非线性结构使流浪者无法离开其它区域抵达远处的山脉区域。**Level Dark 0**中不存在昼夜更替，因此，该楼层一直处于黑夜状态，不过，流浪者没有必要过于担心照明问题，该楼层的月亮发出的光芒足以流浪者看清道路，但目前仍然不清楚这些月光究竟是月亮对光的反射，还是月亮本身就是光源。

每个进入**Level Dark 0**的流浪者首先都会到达楼层的沿岸区域。在沿岸区域里，流浪者可以通常会发现两种路径，第一种路径类似于乡间小道，其由石子，沙子，泥土或青草构成，这些材质的分布被认为是完全混乱的；而第二种路径则类似于前室中的公路，相比第一种路径，路灯更有可能刷新于此。沿岸区域上可能会随机刷新一些树木，当前，这些树木无法用任何已知的手段破坏；对于流浪者而言，看到这些树木是件好事，因为树木旁往往会出现珍贵的生存物资。沿岸区域很少会出现路灯，这些路灯一般相隔都很近。流浪者如果跟着这些路灯走，最终就会到达人造区域。

该楼层中，人造区域十分罕见，通常情况下只有流浪者寻找得足够久才能到达。经证实，人造区域的空间大小是有限的，但是，因其非线性结构，经常会给流浪者带来走不到尽头的感觉。人造区域就像被故意分割开了一样，其地面被划分为了许多块。人造区域内规律的排列着路灯，因此，位处人造区域的流浪者完全不需要担心光亮问题。该区域中，靠近湖泊的部分还会整齐地排列着椅子，可供流浪者短暂休息。人造区域中的声音受到了严重的干扰，其主要表现为过于死寂的宁静或突然传来的震耳欲聋的噪声，同时，流浪者自己的声音也会受到干扰。

该楼层的湖泊与大多数湖泊都极其相似，无论是外观，还是其他的特征。但是，未经M.E.G.Dark组织成员的允许，任何人都不得进入**Level Dark 0**的湖泊区域，因为该楼层的湖泊内部酸性物质已经严重超标。要是流浪者足够幸运，只是短时间将身体浸入湖泊，可能就只会造成剧烈的麻木感和轻微的皮肤灼伤；但如果浸入时间过长，就必定会给流浪者造成无法逆转的伤害，如皮肤腐烂，肢体掉落，甚至是直接导致内部器官衰竭或死亡。

实体
--

该楼层似乎缺乏实体迹象，因为截止目前为止，仍未发现任何实体。

## 据点和前哨站

### M.E.G.Dark勘探队

* 由16名成员组成；
* 致力于探索**Level Dark 0**；
* 友好且开放贸易。

## 入口和出口

### 入口

* 卡出集群Dark中的许多楼层都能来到**Level Dark 0**。
* 卡出集群I或集群ZH任意楼层的暗处，都有较小概率抵达该楼层。

### 出口

* 该楼层只能通过卡出离开，之后流浪者便会到达集群Dark中的随机楼层。但在每次卡出前都请三思而后行，因为前方极有可能是从未被其他人类探索过的后室一角。

**> 查看著作信息**

**> 收起著作信息**

**作者：**XiaoLu408
**附注：**祝您阅读愉快！
Humanized Non-IgG Antibodies Engineering Service

Antibodies have become an effective tool in various human diseases treatment and diagnosis, and they play an essential role in the remarkable progress of antibodies as therapeutic molecules. Antibody humanization is necessary and of great significance when these therapeutic monoclonal antibodies are produced from non-human systems. It can decrease the immunogenicity, improve its pharmacokinetics *in vivo*, and maintain high affinity and specificity of monoclonal antibodies (mAbs) by antibody humanization engineering.

Heretofore, different methods and techniques have been developed for the humanization of non-human antibodies to improve their biological properties. Currently, antibody humanization can be conducted by several engineering techniques, such as complementarity determining the region (CDR) grafting, chain shuffling, resurfacing and antibody library selections.

With the development of research on bioactive molecules, not only IgG mAbs but also non-IgG antibodies play an increasingly important role in the diagnosis and treatment of diseases. It is necessary to develop humanization engineering techniques for non-IgG therapeutic antibodies. At Creative Biolabs, high-quality non-IgG antibodies (mainly IgA, IgM, and IgE) are generated by our Expression System Platform through DNA recombinant technologies. For non-IgG therapeutic antibodies that are obtained from non-human expression systems, we optimize them through humanization engineering technique to enhance their performance.
Human Macrophage Culture

Macrophages are able to initiate a particular type of inflammatory response *via* the release of a certain pattern of cytokines. They play a major role as immunoregulatory cells. Creative Biolabs has built a highly experienced team of scientists and quality staff that have a long history in macrophage isolation and culture. Human monocyte, human alveolar, murine peritoneal cavity, murine bone marrow, murine lung, and murine adipose tissues are available for macrophage isolation and culture. Our seasoned scientists are pleased to share our expertise and experience with our global clients and facilitate their meaningful macrophage project development.

Creative Biolabs combines deep, industry-leading expertise with an innovative Macrophage Therapeutics Development Platform to provide high-quality human/murine macrophage isolation and culture services. These isolated macrophages can be examined *in vitro* for their roles in phagocytosis, cytokine and mediator production, and other biological functions. Our R&D scientific team has accumulated extensive experience in comparing profiles of macrophages between controls and patients, which allows us to effectively determine the changes of the precursor number or the phenotype, with the eventual aim to help our clients develop cell therapies.

Learn more: Human Macrophage Culture
AI-based small molecule design and optimization

### Our Services for Small Molecule Design and Optimization

At Creative Biolabs, we are dedicated to helping our worldwide customers to select, design, and optimize the small-molecule for drug discovery.

For instance, deep learning (DL) methods are commonly used for achieving target recognition of small molecules in drug discovery. These DL models can successfully predict the activity and property of various small molecules. As one of the most popular DL architectures, a convolutional neural network (CNN) has been developed by analyzing the sparse connectivity and shared weights to mimic the interaction between candidate compounds and proteins. Besides, a big data library for training the DL models, including the AlphaGo, has been established to collect enough data for AI-aided intricate simulation.

**Creative Biolabs** has developed a novel AI-based small molecule design and optimization platform to boost the functions of our AI-driven drug discovery pipeline. Our ligand- and/or structure-based databases and focused library approaches will remove the obstacles of your projects and bring breakthroughs to affect the future of small-molecule drug discovery.