Enzyme
stringlengths 4
59
| Organism
stringlengths 1
70
| Substrate
stringlengths 1
86
| km
float64 0
1.4M
⌀ | Unit_Km
stringclasses 65
values | Kcat
stringlengths 1
16
| Unit_Kcat
stringclasses 134
values | Kcat/Km
stringlengths 1
21
| Unit_Kcat/Km
stringclasses 335
values | Commentary[Temp]
stringclasses 165
values | Commentary[pH]
stringclasses 225
values | Commentary[Mutant]
stringlengths 1
93
| Commentary[Cosubstrate]
stringlengths 1
89
| pubmed id
float64 1.5M
35.5M
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TruB | Escherichia coli | tRNA | 1,610 | nM | 0.012 | s^-1 | 7.5 × 10^3 | M^-1s^-1 | 37°C | 7.5 | K19M | 10,924,141 |
|
TruB | Escherichia coli | tRNA | 900 | nM | 0.011 | s^-1 | 1.2 × 10^4 | M^-1s^-1 | 37°C | 7.5 | K19R | 10,924,141 |
|
TruB | Escherichia coli | tRNA | 324 | nM | 0.23 | s^-1 | 7.1 × 10^5 | M^-1s^-1 | 37°C | 7.5 | P20G | 10,924,141 |
|
TruB | Escherichia coli | tRNA | 517 | nM | 0.52 | s^-1 | 1.0 × 10^6 | M^-1s^-1 | 37°C | 7.5 | P20L | 10,924,141 |
|
RluA | Escherichia coli | tRNA | 108 | nM | 0.099 | s^-1 | 9.2 × 10^5 | M^-1s^-1 | 37°C | 7.5 | Wild-type | 10,924,141 |
|
RluA | Escherichia coli | tRNA | 318 | nM | 0.061 | s^-1 | 1.9 × 10^5 | M^-1s^-1 | 37°C | 7.5 | P29G | 10,924,141 |
|
FKBP | Halobacterium cutirubrum | N-suc-A-L-P-F-pNA | null | 0.78 | mM^-1s^-1 | 15°C | 2.9 M KCl | 11,054,562 |
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FKBP | Halobacterium cutirubrum | N-suc-A-A-P-F-pNA | null | 0.15 | mM^-1s^-1 | 15°C | 2.9 M KCl | 11,054,562 |
|||||
FKBP | Thermococcus KS1 | N-suc-A-L-P-F-pNA | null | 350 | mM^-1s^-1 | 15°C | 11,054,562 |
||||||
FKBP | Thermococcus KS1 | N-suc-A-A-P-F-pNA | null | 290 | mM^-1s^-1 | 15°C | 11,054,562 |
||||||
FKBP | Methanococcus thermolithotrophicus | N-suc-A-L-P-F-pNA | null | 350 | mM^-1s^-1 | 15°C | 11,054,562 |
||||||
FKBP | Methanococcus thermolithotrophicus | N-suc-A-A-P-F-pNA | null | 200 | mM^-1s^-1 | 15°C | 11,054,562 |
||||||
Trigger factor | Escherichia coli | N-suc-A-L-P-F-pNA | null | 430 | mM^-1s^-1 | 10°C | 11,054,562 |
||||||
Trigger factor | Escherichia coli | N-suc-A-A-P-F-pNA | null | 160 | mM^-1s^-1 | 10°C | 11,054,562 |
||||||
FKBP | Bovine | N-suc-A-L-P-F-pNA | null | 640 | mM^-1s^-1 | 10°C | 11,054,562 |
||||||
FKBP | Bovine | N-suc-A-A-P-F-pNA | null | 50 | mM^-1s^-1 | 10°C | 11,054,562 |
||||||
CyP18 | Escherichia coli | N-suc-A-L-P-F-pNA | null | 23400 | mM^-1s^-1 | 15°C | 11,054,562 |
||||||
VibE | Vibrio cholerae | 2,3-DHB | 0.46 | µM | 138 | min^-1 | 5 × 10^6 | s^-1M^-1 | ATP | 11,112,537 |
|||
VibE | Vibrio cholerae | salicylate | 16 | µM | 29.3 | min^-1 | 3 × 10^4 | s^-1M^-1 | ATP | 11,112,537 |
|||
EntE | Escherichia coli | 2,3-DHB | 2.7 | µM | 330 | min^-1 | 2 × 10^6 | s^-1M^-1 | ATP | 11,112,537 |
|||
EntE | Escherichia coli | salicylate | 91 | µM | 150 | min^-1 | 3 × 10^4 | s^-1M^-1 | ATP | 11,112,537 |
|||
YbtE | Yersinia pestis | 2,3-DHB | 400 | µM | 14 | min^-1 | 6 × 10^2 | s^-1M^-1 | ATP | 11,112,537 |
|||
YbtE | Yersinia pestis | salicylate | 4.6 | µM | 230 | min^-1 | 8 × 10^5 | s^-1M^-1 | ATP | 11,112,537 |
|||
VibE | Vibrio cholerae | DHB | 3 | µM | 20 | min^-1 | VibB | 11,112,537 |
|||||
VibE | Vibrio cholerae | salicylate | 2 | µM | 5 | min^-1 | VibB | 11,112,537 |
|||||
VibE | Vibrio cholerae | salicylate | 18.6 | µM | 4.0 | min^-1 | EntB | 11,112,537 |
|||||
VibH | Vibrio cholerae | norspermidine | 1.5 | mM | 430 | min^-1 | 4.8 × 10^3 | s^-1M^-1 | DHB-S-VibB | 11,112,537 |
|||
VibH | Vibrio cholerae | 1,7-diaminoheptane | 6.2 | mM | 697 | min^-1 | 1.9 × 10^3 | s^-1M^-1 | DHB-S-VibB | 11,112,537 |
|||
VibH | Vibrio cholerae | octylamine | 30 | mM | 157 | min^-1 | 87 | s^-1M^-1 | DHB-S-VibB | 11,112,537 |
|||
VibH | Vibrio cholerae | hexylamine | 192 | mM | 84 | min^-1 | 7 | s^-1M^-1 | DHB-S-VibB | 11,112,537 |
|||
VibH | Vibrio cholerae | VibB | 0.88 | µM | 600 | min^-1 | 1.1 × 10^7 | s^-1M^-1 | norspermidine | 11,112,537 |
|||
MTH1 | Homo sapiens | 2-OH-rATP | 4.3 | µM | 4.7 | s^-1 | 1.09 | s^-1 µM^-1 | 30°C | 8.0 | 11,139,615 |
||
MTH1 | Homo sapiens | 8-OH-rGTP | 55 | µM | 2.6 | s^-1 | 0.05 | s^-1 µM^-1 | 30°C | 8.0 | 11,139,615 |
||
MTH1 | Homo sapiens | 8-OH-rATP | 51 | µM | 1.1 | s^-1 | 0.02 | s^-1 µM^-1 | 30°C | 8.0 | 11,139,615 |
||
MTH1 | Homo sapiens | 2-OH-dATP | 8.3 | µM | 13.9 | s^-1 | 1.68 | s^-1 µM^-1 | 30°C | 8.0 | 11,139,615 |
||
MTH1 | Homo sapiens | 8-OH-dGTP | 15.2 | µM | 12.3 | s^-1 | 0.81 | s^-1 µM^-1 | 30°C | 8.0 | 11,139,615 |
||
MTH1 | Homo sapiens | 8-OH-dATP | 13.9 | µM | 10.8 | s^-1 | 0.78 | s^-1 µM^-1 | 30°C | 8.0 | 11,139,615 |
||
tHisF | Thermotoga maritima | PRFAR | 1.7 | μM | 2.2 | s^-1 | 1.3 | μM^-1s^-1 | 25°C | 8.5 | Wild-type | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | NH3 | 2.2 | mM | 2.0 | s^-1 | 0.9 | mM^-1s^-1 | 25°C | 8.5 | Wild-type | PRFAR (50 μM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | PRFAR | 1.5 | μM | 0.8 | s^-1 | 0.6 | μM^-1s^-1 | 25°C | 8.0 | Wild-type | Glutamine (5 mM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | Glutamine | 0.32 | mM | 0.4 | s^-1 | 1.4 | mM^-1s^-1 | 25°C | 8.0 | Wild-type | PRFAR (50 μM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | Glutamine | 0.5 | mM | 0.1 | s^-1 | 0.2 | mM^-1s^-1 | 25°C | 8.0 | Wild-type | ImGP | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | Glutamine | null | 0.1 | s^-1 | 25°C | 8.0 | Wild-type | ProFAR | 11,264,293 |
|||
tHisF | Thermotoga maritima | PRFAR | 5.7 | μM | 1.8 | s^-1 | 0.32 | μM^-1s^-1 | 25°C | 8.5 | C9A | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | NH3 | 7.3 | mM | 1.8 | s^-1 | 0.24 | mM^-1s^-1 | 25°C | 8.5 | C9A | PRFAR (50 μM) | 11,264,293 |
tHisF | Thermotoga maritima | PRFAR | 1.7 | μM | 0.000037 | s^-1 | 0.000022 | μM^-1s^-1 | 25°C | 8.5 | D11N | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | PRFAR | 8.5 | μM | 2.2 | s^-1 | 0.26 | μM^-1s^-1 | 25°C | 8.5 | K19S | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | NH3 | 5.5 | mM | 1.7 | s^-1 | 0.3 | mM^-1s^-1 | 25°C | 8.5 | K19S | PRFAR (50 μM) | 11,264,293 |
tHisF | Thermotoga maritima | PRFAR | 2.8 | μM | 1.0 | s^-1 | 0.35 | μM^-1s^-1 | 25°C | 8.5 | D51N | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | NH3 | 22 | mM | 2.0 | s^-1 | 0.09 | mM^-1s^-1 | 25°C | 8.5 | D51N | PRFAR (50 μM) | 11,264,293 |
tHisF | Thermotoga maritima | PRFAR | 2.8 | μM | 2.2 | s^-1 | 0.76 | μM^-1s^-1 | 25°C | 8.5 | N103A | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | NH3 | 7.3 | mM | 2.8 | s^-1 | 0.38 | mM^-1s^-1 | 25°C | 8.5 | N103A | PRFAR (50 μM) | 11,264,293 |
tHisF | Thermotoga maritima | PRFAR | 28.3 | μM | 0.005 | s^-1 | 0.00018 | μM^-1s^-1 | 25°C | 8.5 | D130E | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | NH3 | 3.7 | mM | 0.003 | s^-1 | 0.0009 | mM^-1s^-1 | 25°C | 8.5 | D130E | PRFAR (50 μM) | 11,264,293 |
tHisF | Thermotoga maritima | PRFAR | 850 | μM | 0.004 | s^-1 | 0.0000043 | μM^-1s^-1 | 25°C | 8.5 | D130N | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | PRFAR | 34 | μM | 0.046 | s^-1 | 0.0014 | μM^-1s^-1 | 25°C | 8.5 | D176N | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | NH3 | 4.4 | mM | 0.044 | s^-1 | 0.01 | mM^-1s^-1 | 25°C | 8.5 | D176N | PRFAR (50 μM) | 11,264,293 |
tHisF | Thermotoga maritima | PRFAR | 2.8 | μM | 1.1 | s^-1 | 0.39 | μM^-1s^-1 | 25°C | 8.5 | D183N | NH3 (100 mM NH4 acetate) | 11,264,293 |
tHisF | Thermotoga maritima | NH3 | 5.5 | mM | 2.0 | s^-1 | 0.35 | mM^-1s^-1 | 25°C | 8.5 | D183N | PRFAR (50 μM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | PRFAR | 3.8 | μM | 0.9 | s^-1 | 0.26 | μM^-1s^-1 | 25°C | 8.0 | C9A | Glutamine (5 mM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | Glutamine | 0.64 | mM | 0.6 | s^-1 | 1.0 | mM^-1s^-1 | 25°C | 8.0 | C9A | PRFAR (50 μM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | PRFAR | 30 | μM | 0.057 | s^-1 | 0.0022 | μM^-1s^-1 | 25°C | 8.0 | K19S | Glutamine (5 mM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | Glutamine | 1.6 | mM | 0.08 | s^-1 | 0.056 | mM^-1s^-1 | 25°C | 8.0 | K19S | PRFAR (50 μM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | PRFAR | 1 | μM | 0.057 | s^-1 | 0.067 | μM^-1s^-1 | 25°C | 8.0 | D51N | Glutamine (5 mM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | Glutamine | 0.32 | mM | 0.05 | s^-1 | 0.18 | mM^-1s^-1 | 25°C | 8.0 | D51N | PRFAR (50 μM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | PRFAR | 0.45 | μM | 0.19 | s^-1 | 0.46 | μM^-1s^-1 | 25°C | 8.0 | N103A | Glutamine (5 mM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | Glutamine | 0.25 | mM | 0.2 | s^-1 | 0.93 | mM^-1s^-1 | 25°C | 8.0 | N103A | PRFAR (50 μM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | PRFAR | 15 | μM | 0.003 | s^-1 | 0.0002 | μM^-1s^-1 | 25°C | 8.0 | D130E | Glutamine (5 mM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | PRFAR | 0.83 | μM | 0.02 | s^-1 | 0.026 | μM^-1s^-1 | 25°C | 8.0 | D176N | Glutamine (5 mM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | Glutamine | 0.21 | mM | 0.009 | s^-1 | 0.048 | mM^-1s^-1 | 25°C | 8.0 | D176N | PRFAR (50 μM) | 11,264,293 |
tHisH-tHisF | Thermotoga maritima | PRFAR | 2.1 | μM | 0.099 | s^-1 | 0.05 | μM^-1s^-1 | 25°C | 8.0 | D183N | Glutamine (5 mM) | 11,264,293 |
Prolyl oligopeptidase | Pyrococcus furiosus | Z-Ala-Pro-pNA | 43.7 | µM | 17.9 | s^-1 | 410 | s^-1mM^-1 | 85°C | 7.5 | 0.1 M NaCl | 11,278,687 |
|
Prolyl oligopeptidase | Pyrococcus furiosus | Z-Gly-Pro-pNA | 160 | µM | 16.9 | s^-1 | 106 | s^-1mM^-1 | 85°C | 7.5 | 0.1 M NaCl | 11,278,687 |
|
Prolyl oligopeptidase | Pyrococcus furiosus | Z-Ala-Ala-pNA | 43 | µM | 2.50 | s^-1 | 57.6 | s^-1mM^-1 | 85°C | 7.5 | 0.1 M NaCl | 11,278,687 |
|
Prolyl oligopeptidase | Pyrococcus furiosus | H-Ala-Pro-pNA | 19.4 | µM | 0.079 | s^-1 | 4.08 | s^-1mM^-1 | 85°C | 7.5 | 0.1 M NaCl | 11,278,687 |
|
Prolyl oligopeptidase | Pyrococcus furiosus | H-Gly-Pro-pNA | 20.7 | µM | 0.060 | s^-1 | 2.90 | s^-1mM^-1 | 85°C | 7.5 | 0.1 M NaCl | 11,278,687 |
|
Prolyl oligopeptidase | Pyrococcus furiosus | H-Asp-Pro-pNA | 40 | µM | 0.073 | s^-1 | 1.82 | s^-1mM^-1 | 85°C | 7.5 | 0.1 M NaCl | 11,278,687 |
|
hITPase | Homo sapiens | ITP | 0.51 | mM | 580 | s^-1 | 1140 | 10^3 M^-1s^-1 | 37°C | 8.5 | 50 mM MgCl2, 1 mM DTT | 11,278,832 |
|
hITPase | Homo sapiens | dITP | 0.31 | mM | 360 | s^-1 | 1160 | 10^3 M^-1s^-1 | 37°C | 8.5 | 50 mM MgCl2, 1 mM DTT | 11,278,832 |
|
hITPase | Homo sapiens | XTP | 0.57 | mM | 640 | s^-1 | 1120 | 10^3 M^-1s^-1 | 37°C | 8.5 | 50 mM MgCl2, 1 mM DTT | 11,278,832 |
|
GtfB | Amycolatopsis orientalis (A82846) | UDP-glucose | 1.3 | mM | 17 | min^-1 | 37°C | 9 | 11,294,642 |
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GtfB | Amycolatopsis orientalis (A82846) | Vancomycin aglycone (4) | 250 | µM | 17 | min^-1 | 37°C | 9 | 11,294,642 |
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GtfE | Amycolatopsis orientalis ATCC19795 | Vancomycin aglycone (4) | 1.5 | mM | 60 | min^-1 | 37°C | 9 | 11,294,642 |
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GtfE | Amycolatopsis orientalis ATCC19795 | Teicoplanin aglycone (6) | null | 20 | min^-1 | 37°C | 9 | 11,294,642 |
|||||
GtfB | Amycolatopsis orientalis (A82846) | Teicoplanin aglycone (6) | null | 0.3 | min^-1 | 37°C | 9 | 11,294,642 |
|||||
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose 5-phosphate | 76 | µM | 22 | s^-1 | 2.9 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 85 µM CTP, 10 mM MgCl2 | 11,305,920 |
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-arabinose 5-phosphate | 3,000 | µM | 2.3 | s^-1 | 0.0077 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 85 µM CTP, 10 mM MgCl2 | 11,305,920 |
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-erythrose 4-phosphate | 38 | µM | 0.5 | s^-1 | 0.13 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 85 µM CTP, 10 mM MgCl2 | 11,305,920 |
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribose 5-phosphate | 2,200 | µM | 4.1 | s^-1 | 0.019 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 85 µM CTP, 10 mM MgCl2 | 11,305,920 |
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose | 1,100 | µM | 3.6 | s^-1 | 0.033 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 85 µM CTP, 10 mM MgCl2 | 11,305,920 |
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-xylulose 5-phosphate | null | µM | 3.6 | s^-1 | <0.010 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 85 µM CTP, 10 mM MgCl2 | 11,305,920 |
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | NADPH | 44 | µM | 22 | s^-1 | 5.0 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 85 µM CTP, 10 mM MgCl2 | 11,305,920 |
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | NADH | null | µM | 0.064 | s^-1 | <0.0011 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 85 µM CTP, 10 mM MgCl2 | 11,305,920 |
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribitol 5-phosphate | 130 | µM | 13 | s^-1 | 1.0 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-erythrose 4-phosphate | null | µM | 0.15 | s^-1 | <0.00030 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-galactose 6-phosphate | null | µM | 0.21 | s^-1 | <0.00042 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribose 5-phosphate | null | µM | 0.52 | s^-1 | <0.0010 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose 5-phosphate | null | µM | 0.77 | s^-1 | <0.0015 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | CTP | 99 | µM | 13 | s^-1 | 1.3 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | Wild type | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose 5-phosphate | 270 | µM | 6.8 | s^-1 | 0.25 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | R18A | 11,305,920 |