Enzyme
stringlengths 4
59
| Organism
stringlengths 1
70
| Substrate
stringlengths 1
86
| km
float64 0
1.4M
⌀ | Unit_Km
stringclasses 65
values | Kcat
stringlengths 1
16
| Unit_Kcat
stringclasses 134
values | Kcat/Km
stringlengths 1
21
| Unit_Kcat/Km
stringclasses 335
values | Commentary[Temp]
stringclasses 165
values | Commentary[pH]
stringclasses 225
values | Commentary[Mutant]
stringlengths 1
93
| Commentary[Cosubstrate]
stringlengths 1
89
| pubmed id
float64 1.5M
35.5M
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
His-BGPh | Pyrococcus horikoshii | p-Nph-β-D-Galp | 1.3 | mM | 123 | s^-1 | 94.34 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | p-Nph-β-D-Xylp | 0.1 | mM | 3 | s^-1 | 31.83 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | p-Nph-β-D-Manp | 0.14 | mM | 2 | s^-1 | 14.60 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | Salicin | 1.96 | mM | 44 | s^-1 | 22.20 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | Methyl-β-D-Glcp | 40.74 | mM | 35 | s^-1 | 0.85 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | n-Amyl-β-D-Glcp | 2.02 | mM | 31 | s^-1 | 15.11 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | n-Hexyl-β-D-Glcp | 0.54 | mM | 33 | s^-1 | 60.28 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | n-Octyl-β-D-Glcp | 0.2 | mM | 34 | s^-1 | 170.70 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | n-Nonyl-β-D-Glcp | 0.08 | mM | 39 | s^-1 | 471.57 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | n-Decyl-β-D-Glcp | 0.08 | mM | 37 | s^-1 | 469.62 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | n-Undecyl-β-D-Glcp | 0.05 | mM | 43 | s^-1 | 944.37 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
His-BGPh | Pyrococcus horikoshii | n-Dodecyl-β-D-Glcp | 0.03 | mM | 36 | s^-1 | 1152.90 | mM^-1s^-1 | 90°C | 6.0 | His-tagged | 0.1% Triton X-100, 0.3 M NaCl | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | Laminaribiose | 1 | mM | 908 | s^-1 | 908.0 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | Cellobiose | 30 | mM | 1333 | s^-1 | 44.4 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | Cellotriose | 3 | mM | 197 | s^-1 | 66 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | Cellotetraose | 1.7 | mM | 584 | s^-1 | 343 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | β-Gentiobiose | 100 | mM | 1360 | s^-1 | 14 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | p-Nph-β-D-Glcp | 0.5 | mM | 542 | s^-1 | 1084.0 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | p-Nph-β-D-Galp | 4.7 | mM | 1020 | s^-1 | 217.0 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | p-Nph-β-D-Xylp | 4 | mM | 284 | s^-1 | 71.0 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | Salicin | 5 | mM | 880 | s^-1 | 175.9 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | n-Amyl-β-D-Glcp | 1.1 | mM | 256 | s^-1 | 232 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
Sβ-gly | Sulfolobus solfataricus | n-Hexyl-β-D-Glcp | 1 | mM | 263 | s^-1 | 263 | mM^-1s^-1 | 75°C | 6.5 | Wild-type | Not specified | 10,675,537 |
ALR1 | Candida intermedia | NADPH | 61 | µM | 14.6 | s^-1 | 2.4 × 10^5 | M^-1 s^-1 | 25°C | 7.0 | D-Xylose (707 mM) | 10,681,056 |
|
ALR1 | Candida intermedia | D-Xylose | 82 | mM | 14.6 | s^-1 | 178 | M^-1 s^-1 | 25°C | 7.0 | NADPH (0.22 mM) | 10,681,056 |
|
ALR2 | Candida intermedia | NADH | 28 | µM | 11.2 | s^-1 | 4 × 10^5 | M^-1 s^-1 | 25°C | 7.0 | D-Xylose (707 mM) | 10,681,056 |
|
ALR2 | Candida intermedia | NADPH | 56 | µM | 11 | s^-1 | 2 × 10^5 | M^-1 s^-1 | 25°C | 7.0 | D-Xylose (707 mM) | 10,681,056 |
|
ALR2 | Candida intermedia | D-Xylose | 50 | mM | 10.5 | s^-1 | 210 | M^-1 s^-1 | 25°C | 7.0 | NADH (0.22 mM) | 10,681,056 |
|
ALR2 | Candida intermedia | D-Xylose | 52 | mM | 9.8 | s^-1 | 188 | M^-1 s^-1 | 25°C | 7.0 | NADPH (0.22 mM) | 10,681,056 |
|
EcSßgly | Sulfolobus solfataricus | ONPG | 4.1 | mM | 1689 | s^-1 | 411.95 | s^-1mM^-1 | 75°C | Free enzyme | 10,682,286 |
||
EcSßgly | Sulfolobus solfataricus | ONPG | 5.3 | mM | 1662 | s^-1 | 313.6 | s^-1mM^-1 | 75°C | Immobilised enzyme | 10,682,286 |
||
EcSßgly | Sulfolobus solfataricus | Cellobiose | 32.7 | mM | 628 | s^-1 | 19.20 | s^-1mM^-1 | 75°C | Free enzyme | 10,682,286 |
||
EcSßgly | Sulfolobus solfataricus | Cellobiose | 21.6 | mM | 727 | s^-1 | 33.7 | s^-1mM^-1 | 75°C | Immobilised enzyme | 10,682,286 |
||
EcSßgly | Sulfolobus solfataricus | Oleuropein | 240.4 | mM | 350.8 | s^-1 | 1.46 | s^-1mM^-1 | 70°C | Free enzyme | 10,682,286 |
||
AtOMT1 | Arabidopsis thaliana | Quercetin | 1.76 | µM | 384.6 | pkat/mg | 0.016 | µM^-1s^-1 | 30°C | 7.5 | S-adenosyl-L-methionine | 10,700,397 |
|
AtOMT1 | Arabidopsis thaliana | Myricetin | 3.38 | µM | 227.3 | pkat/mg | 0.003 | µM^-1s^-1 | 30°C | 7.5 | S-adenosyl-L-methionine | 10,700,397 |
|
UBGAT | Scutellaria baicalensis | UDP-glucuronic acid | 72.9 | µM | 30-40°C | 7.5 | 10,724,177 |
||||||
UBGAT | Scutellaria baicalensis | Baicalein | 52.4 | µM | 257 | nkat mg^-1 | 4.90 | 30-40°C | 7.5 | 10,724,177 |
|||
UBGAT | Scutellaria baicalensis | Scutellarein | 109 | µM | 290 | nkat mg^-1 | 2.67 | 30-40°C | 7.5 | 10,724,177 |
|||
UBGAT | Scutellaria baicalensis | Wogonin | 106.5 | µM | 182 | nkat mg^-1 | 1.71 | 30-40°C | 7.5 | 10,724,177 |
|||
ADH γγ isozyme | Homo sapiens | isoLCA | 18.3 | μmol/L | 0.9 | min^-1 | 0.05 | min^-1 × μmol/L^-1 | 25°C | 10 | NAD^+ 2.5 mmol/L | 10,733,557 |
|
ADH γγ isozyme | Homo sapiens | isoDCA | 28.3 | μmol/L | 2.8 | min^-1 | 0.10 | min^-1 × μmol/L^-1 | 25°C | 10 | NAD^+ 2.5 mmol/L | 10,733,557 |
|
ADH γγ isozyme | Homo sapiens | isoCDCA | 15.3 | μmol/L | 1.3 | min^-1 | 0.09 | min^-1 × μmol/L^-1 | 25°C | 10 | NAD^+ 2.5 mmol/L | 10,733,557 |
|
ADH γγ isozyme | Homo sapiens | isoUDCA | 23.8 | μmol/L | 2 | min^-1 | 0.08 | min^-1 × μmol/L^-1 | 25°C | 10 | NAD^+ 2.5 mmol/L | 10,733,557 |
|
ADH γγ isozyme | Homo sapiens | Ethanol | 490 | μmol/L | 50 | min^-1 | 0.10 | min^-1 × μmol/L^-1 | 25°C | 10 | NAD^+ 2.5 mmol/L | 10,733,557 |
|
IGP synthase (HIS7) | Saccharomyces cerevisiae | PRFAR | 4 | µM | 3.9 | s^-1 | 9.5 × 10^5 | M^-1s^-1 | 30°C | 7.0 | Wild-type | Glutamine (40 mM) | 10,733,892 |
IGP synthase (HIS7) | Saccharomyces cerevisiae | Glutamine | 1.7 | mM | 5.2 | s^-1 | 3.1 × 10^3 | M^-1s^-1 | 30°C | 7.0 | Wild-type | PRFAR (100 µM) | 10,733,892 |
IGP synthase (HIS7) | Saccharomyces cerevisiae | NH4Cl | 246 | mM | 0.6 | s^-1 | 2.4 | M^-1s^-1 | 30°C | 8.5 | Wild-type | PRFAR (100 µM) | 10,733,892 |
IGP synthase (HisF-HisH) | Escherichia coli | PRFAR | 1.5 | µM | 8.5 | s^-1 | 5.7 × 10^6 | M^-1s^-1 | 30°C | 8.0 | Wild-type | Glutamine (saturating) | 10,733,892 |
IGP synthase (HisF-HisH) | Escherichia coli | Glutamine | 240 | µM | 9.1 | s^-1 | 3.8 × 10^4 | M^-1s^-1 | 30°C | 8.0 | Wild-type | PRFAR (saturating) | 10,733,892 |
IGP synthase (HisF-HisH) | Escherichia coli | NH4Cl | 291 | mM | 8.8 | s^-1 | 30 | M^-1s^-1 | 30°C | 8.0 | Wild-type | PRFAR (saturating) | 10,733,892 |
IGP synthase (HIS7) | Saccharomyces cerevisiae | Glutamine | 3.5 | mM | 0.006 | s^-1 | 1.7 | s^-1M^-1 | 30°C | 7.0 | Wild-type | None | 10,733,892 |
IGP synthase (HIS7) | Saccharomyces cerevisiae | Glutamine | 5.4 | mM | 5 | s^-1 | 926 | s^-1M^-1 | 30°C | 7.0 | Wild-type | 5'-ProFAR (2 mM) | 10,733,892 |
IGP synthase (HisF-HisH) | Escherichia coli | Glutamine | 4.8 | mM | 0.07 | s^-1 | 14.6 | s^-1M^-1 | 30°C | 8.0 | Wild-type | None | 10,733,892 |
CelB | Pyrococcus furiosus | pNp-Glc | 0.53 | mM | 13 | s^-1 | 25 | mM^-1s^-1 | 20°C | 4.8 | Wild-type | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Gal | 0.7 | mM | 9.1 | s^-1 | 13 | mM^-1s^-1 | 20°C | 4.8 | Wild-type | 10,736,164 |
|
CelB | Pyrococcus furiosus | Cellobiose | 23 | mM | 16 | s^-1 | 0.69 | mM^-1s^-1 | 20°C | 4.8 | Wild-type | 10,736,164 |
|
CelB | Pyrococcus furiosus | Lactose | 52 | mM | 13 | s^-1 | 0.25 | mM^-1s^-1 | 20°C | 4.8 | Wild-type | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Glc | 1.1 | mM | 47 | s^-1 | 43 | mM^-1s^-1 | 20°C | 4.8 | N415S | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Gal | 1.2 | mM | 4.2 | s^-1 | 3.5 | mM^-1s^-1 | 20°C | 4.8 | N415S | 10,736,164 |
|
CelB | Pyrococcus furiosus | Cellobiose | 20 | mM | 20 | s^-1 | 1.0 | mM^-1s^-1 | 20°C | 4.8 | N415S | 10,736,164 |
|
CelB | Pyrococcus furiosus | Lactose | 95 | mM | 21 | s^-1 | 0.22 | mM^-1s^-1 | 20°C | 4.8 | N415S | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Glc | 0.92 | mM | 40 | s^-1 | 43 | mM^-1s^-1 | 20°C | 4.8 | M424V | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Gal | 3.4 | mM | 12 | s^-1 | 3.4 | mM^-1s^-1 | 20°C | 4.8 | M424V | 10,736,164 |
|
CelB | Pyrococcus furiosus | Cellobiose | 16 | mM | 18 | s^-1 | 1.1 | mM^-1s^-1 | 20°C | 4.8 | M424V | 10,736,164 |
|
CelB | Pyrococcus furiosus | Lactose | 97 | mM | 21 | s^-1 | 0.22 | mM^-1s^-1 | 20°C | 4.8 | M424V | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Glc | 0.57 | mM | 26 | s^-1 | 45 | mM^-1s^-1 | 20°C | 4.8 | T371A | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Gal | 1.3 | mM | 11 | s^-1 | 8.2 | mM^-1s^-1 | 20°C | 4.8 | T371A | 10,736,164 |
|
CelB | Pyrococcus furiosus | Cellobiose | 11 | mM | 15 | s^-1 | 1.4 | mM^-1s^-1 | 20°C | 4.8 | T371A | 10,736,164 |
|
CelB | Pyrococcus furiosus | Lactose | 52 | mM | 17 | s^-1 | 0.33 | mM^-1s^-1 | 20°C | 4.8 | T371A | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Glc | 1.3 | mM | 40 | s^-1 | 31 | mM^-1s^-1 | 20°C | 4.8 | A419T | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Gal | 4.5 | mM | 14 | s^-1 | 3.1 | mM^-1s^-1 | 20°C | 4.8 | A419T | 10,736,164 |
|
CelB | Pyrococcus furiosus | Cellobiose | 25 | mM | 19 | s^-1 | 0.75 | mM^-1s^-1 | 20°C | 4.8 | A419T | 10,736,164 |
|
CelB | Pyrococcus furiosus | Lactose | 83 | mM | 15 | s^-1 | 0.18 | mM^-1s^-1 | 20°C | 4.8 | A419T | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Glc | 0.42 | mM | 1600 | s^-1 | 3900 | mM^-1s^-1 | 90°C | 4.8 | Wild-type | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Gal | 5 | mM | 2400 | s^-1 | 480 | mM^-1s^-1 | 90°C | 4.8 | Wild-type | 10,736,164 |
|
CelB | Pyrococcus furiosus | Cellobiose | 14 | mM | 670 | s^-1 | 48 | mM^-1s^-1 | 90°C | 4.8 | Wild-type | 10,736,164 |
|
CelB | Pyrococcus furiosus | Lactose | 120 | mM | 1300 | s^-1 | 11 | mM^-1s^-1 | 90°C | 4.8 | Wild-type | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Glc | 1.4 | mM | 1400 | s^-1 | 1000 | mM^-1s^-1 | 90°C | 4.8 | N415S | 10,736,164 |
|
CelB | Pyrococcus furiosus | pNp-Gal | 8.5 | mM | 1000 | s^-1 | 120 | mM^-1s^-1 | 90°C | 4.8 | N415S | 10,736,164 |
|
CelB | Pyrococcus furiosus | Cellobiose | 38 | mM | 240 | s^-1 | 6.4 | mM^-1s^-1 | 90°C | 4.8 | N415S | 10,736,164 |
|
Glu1 | Zea mays | pNPGlc | 0.38 | mM | 24.16 | s^-1 | 63.57 | s^-1/mM | 5.8 | 10,748,038 |
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Glu1 | Zea mays | oNPGlc | 1.3 | mM | 19.83 | s^-1 | 15.25 | s^-1/mM | 5.8 | 10,748,038 |
|||
Glu1 | Zea mays | DIMBOAGlc | 0.098 | mM | 29.16 | s^-1 | 297.55 | s^-1/mM | 5.8 | 10,748,038 |
|||
Dhr1 | Sorghum bicolor | dhurrin | 0.05 | mM | 77.70 | s^-1 | 1494.2 | s^-1/mM | 5.8 | 10,748,038 |
|||
Glu1/Dhr1 chimera 2 | Zea mays/Sorghum bicolor | pNPGlc | 0.39 | mM | 107.91 | s^-1 | 276.69 | s^-1/mM | 5.8 | C-terminal 47 aa of Glu1 replaced with 53 aa of Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 2 | Zea mays/Sorghum bicolor | oNPGlc | 1.36 | mM | 64.25 | s^-1 | 47.24 | s^-1/mM | 5.8 | C-terminal 47 aa of Glu1 replaced with 53 aa of Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 2 | Zea mays/Sorghum bicolor | dhurrin | 0.36 | mM | 22.70 | s^-1 | 62.4 | s^-1/mM | 5.8 | C-terminal 47 aa of Glu1 replaced with 53 aa of Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 2 | Zea mays/Sorghum bicolor | DIMBOAGlc | 0.205 | mM | 43.33 | s^-1 | 211.36 | s^-1/mM | 5.8 | C-terminal 47 aa of Glu1 replaced with 53 aa of Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 15 | Zea mays/Sorghum bicolor | pNPGlc | 0.35 | mM | 34.26 | s^-1 | 97.88 | s^-1/mM | 5.8 | C-terminal 23 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 15 | Zea mays/Sorghum bicolor | oNPGlc | 1.39 | mM | 31.19 | s^-1 | 22.43 | s^-1/mM | 5.8 | C-terminal 23 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 15 | Zea mays/Sorghum bicolor | dhurrin | 0.51 | mM | 13.46 | s^-1 | 26.1 | s^-1/mM | 5.8 | C-terminal 23 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 15 | Zea mays/Sorghum bicolor | DIMBOAGlc | 0.106 | mM | 24.02 | s^-1 | 226.60 | s^-1/mM | 5.8 | C-terminal 23 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 16 | Zea mays/Sorghum bicolor | pNPGlc | 0.42 | mM | 90.80 | s^-1 | 216.38 | s^-1/mM | 5.8 | Internal 30 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 16 | Zea mays/Sorghum bicolor | oNPGlc | 1.23 | mM | 54.02 | s^-1 | 43.91 | s^-1/mM | 5.8 | Internal 30 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 16 | Zea mays/Sorghum bicolor | dhurrin | 0.1 | mM | 31.83 | s^-1 | 303.1 | s^-1/mM | 5.8 | Internal 30 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 16 | Zea mays/Sorghum bicolor | DIMBOAGlc | 0.2 | mM | 19.54 | s^-1 | 97.70 | s^-1/mM | 5.8 | Internal 30 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 21 | Zea mays/Sorghum bicolor | pNPGlc | 0.36 | mM | 87.24 | s^-1 | 242.55 | s^-1/mM | 5.8 | 8 aa of Glu1 replaced with Dhr1 | 10,748,038 |
||
Glu1/Dhr1 chimera 21 | Zea mays/Sorghum bicolor | oNPGlc | 1.4 | mM | 37.05 | s^-1 | 26.46 | s^-1/mM | 5.8 | 8 aa of Glu1 replaced with Dhr1 | 10,748,038 |
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Glu1/Dhr1 chimera 21 | Zea mays/Sorghum bicolor | dhurrin | 0.09 | mM | 46.02 | s^-1 | 494.8 | s^-1/mM | 5.8 | 8 aa of Glu1 replaced with Dhr1 | 10,748,038 |