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LLM-Enzyme-Kinetics-Archive-LLENKA

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train · 35.6k rows

Enzyme stringlengths 4 59	Organism stringlengths 1 70	Substrate stringlengths 1 86	km float64 0 1.4M ⌀	Unit_Km stringclasses 65 values	Kcat stringlengths 1 16	Unit_Kcat stringclasses 134 values	Kcat/Km stringlengths 1 21	Unit_Kcat/Km stringclasses 335 values	Commentary[Temp] stringclasses 165 values	Commentary[pH] stringclasses 225 values	Commentary[Mutant] stringlengths 1 93	Commentary[Cosubstrate] stringlengths 1 89	pubmed id float64 1.5M 35.5M ⌀
Glu1/Dhr1 chimera 21	Zea mays/Sorghum bicolor	DIMBOAGlc	0.104	mM	20.15	s^-1	195.19	s^-1/mM		5.8	8 aa of Glu1 replaced with Dhr1		10,748,038
Glu1/Dhr1 chimera 22	Zea mays/Sorghum bicolor	pNPGlc	0.35	mM	68.90	s^-1	196.42	s^-1/mM		5.8	10 aa of Glu1 replaced with Dhr1		10,748,038
Glu1/Dhr1 chimera 22	Zea mays/Sorghum bicolor	oNPGlc	1.02	mM	45.65	s^-1	44.75	s^-1/mM		5.8	10 aa of Glu1 replaced with Dhr1		10,748,038
Glu1/Dhr1 chimera 22	Zea mays/Sorghum bicolor	dhurrin	0.18	mM	27.17	s^-1	150.9	s^-1/mM		5.8	10 aa of Glu1 replaced with Dhr1		10,748,038
Glu1/Dhr1 chimera 22	Zea mays/Sorghum bicolor	DIMBOAGlc	0.098	mM	25.67	s^-1	261.93	s^-1/mM		5.8	10 aa of Glu1 replaced with Dhr1		10,748,038
Dhr1/Glu1 chimera 17	Sorghum bicolor/Zea mays	dhurrin	0.15	mM	18.68	s^-1	124.3	s^-1/mM		5.8	C-terminal 47 aa of Dhr1 replaced with Glu1		10,748,038
Dhr1/Glu1 chimera 23	Sorghum bicolor/Zea mays	dhurrin	0.04	mM	53.96	s^-1	1349.8	s^-1/mM		5.8	C-terminal 16 aa of Dhr1 replaced with Glu1		10,748,038
Dhr1/Glu1 chimera 39	Sorghum bicolor/Zea mays	dhurrin	0.12	mM	23.41	s^-1	195.0	s^-1/mM		5.8	Internal 31 aa of Dhr1 replaced with Glu1		10,748,038
Glu1	Zea mays	dhurrin	0.076	mM						5.8		Competitive inhibitor	10,748,038
RNA triphosphatase	Semliki Forest virus	5'-[γ-32P]RNA2	2.99	µM	5.5	min^-1			25°C	7-8	Nsp2-N		10,748,213
NTPase	Semliki Forest virus	GTP	90	µM	230	min^-1					Nsp2-N		10,748,213
RNA triphosphatase	Semliki Forest virus	5'-[γ-32P]RNA2	null						25°C	7-8	Nsp2		10,748,213
RNA triphosphatase	Semliki Forest virus	5'-[γ-32P]RNA2	null						25°C	7-8	Nsp2-C		10,748,213
RNA triphosphatase	Semliki Forest virus	5'-[γ-32P]RNA2	null						25°C	7-8	Nsp2 (K192N)		10,748,213
RNA triphosphatase	Sindbis virus	5'-[γ-32P]RNA2	null						25°C	7-8	Nsp2		10,748,213
AhpC	Mycobacterium tuberculosis	1+2	2,750	µM	2.3	min^-1	0.8	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpC	Mycobacterium tuberculosis	3	860	µM	0.9	min^-1	1.0	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpC	Mycobacterium tuberculosis	4+5	1,300	µM	2.43	min^-1	1.9	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpC	Mycobacterium tuberculosis	tert-Butyl	1,100	µM	2.1	min^-1	1.9	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpC	Mycobacterium tuberculosis	H2O2	1,500	µM	5.3	min^-1	3.5	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpC	Mycobacterium tuberculosis	PhC(CH3)2OOH	550	µM	2.0	min^-1	3.8	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpC	Mycobacterium tuberculosis	6	91	µM	8.8	min^-1	97	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpD	Mycobacterium tuberculosis	1+2	350	µM	2.7	min^-1	7.7	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpD	Mycobacterium tuberculosis	3	150	µM	0.8	min^-1	5.6	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpD	Mycobacterium tuberculosis	4+5	2,170	µM	4.8	min^-1	2.2	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpD	Mycobacterium tuberculosis	tert-Butyl	230	µM	3.6	min^-1	15.7	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpD	Mycobacterium tuberculosis	H2O2	1,500	µM	5.1	min^-1	3.4	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpD	Mycobacterium tuberculosis	PhC(CH3)2OOH	52	µM	4.0	min^-1	77	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpD	Mycobacterium tuberculosis	6	132	µM	10.7	min^-1	81.4	µM^-1min^-1	25°C	7.0	WT	NADH	10,766,746
AhpC	Mycobacterium tuberculosis	tert-Butyl	null						25°C	7.0	C61S	DTT	10,766,746
AhpC	Mycobacterium tuberculosis	tert-Butyl	null						25°C	7.0	C174S	DTT	10,766,746
AhpC	Mycobacterium tuberculosis	tert-Butyl	null						25°C	7.0	C176S	DTT	10,766,746
AhpD	Mycobacterium tuberculosis	tert-Butyl	null						25°C	7.0	C129S	DTT	10,766,746
sGUS	Scutellaria baicalensis	baicalein 7-O-β-D-glucuronide	12.1	µM	639	s^-1	52.8	µM^-1·s^-1		6.5	Wild-type		10,858,442
sGUS	Scutellaria baicalensis	baicalein 7-O-β-D-glucuronide	10.3	µM	0.135	s^-1	0.0131	µM^-1·s^-1		6.5	E212A		10,858,442
sGUS	Scutellaria baicalensis	baicalein 7-O-β-D-glucuronide	9.7	µM	0.011	s^-1	0.0042	µM^-1·s^-1		6.5	E329A		10,858,442
InhA	Mycobacterium tuberculosis	DDCoA	46	µM	501	min^-1	10.9	µM^-1min^-1	25°C	6.8	Wild type	NADH	10,869,170
InhA	Mycobacterium tuberculosis	NADH	66	µM	501	min^-1			25°C	6.8	Wild type	DDCoA	10,869,170
InhA	Mycobacterium tuberculosis	DDCoA	70	µM	21	min^-1	0.3	µM^-1min^-1	25°C	6.8	Y158F	NADH	10,869,170
InhA	Mycobacterium tuberculosis	NADH	2	µM	21	min^-1			25°C	6.8	Y158F	DDCoA	10,869,170
InhA	Mycobacterium tuberculosis	DDCoA	104	µM	993	min^-1	8.9	µM^-1min^-1	25°C	6.8	S94A	NADH	10,869,170
InhA	Mycobacterium tuberculosis	NADH	250	µM	993	min^-1			25°C	6.8	S94A	DDCoA	10,869,170
InhA	Mycobacterium tuberculosis	DDCoA	36	µM	720	min^-1	20.0	µM^-1min^-1	25°C	6.8	I47T	NADH	10,869,170
InhA	Mycobacterium tuberculosis	NADH	152	µM	720	min^-1			25°C	6.8	I47T	DDCoA	10,869,170
InhA	Mycobacterium tuberculosis	DDCoA	70	µM	925	min^-1	13.2	µM^-1min^-1	25°C	6.8	I21V	NADH	10,869,170
InhA	Mycobacterium tuberculosis	NADH	120	µM	925	min^-1			25°C	6.8	I21V	DDCoA	10,869,170
InhA	Mycobacterium tuberculosis	HXDCoA	10	µM	700	min^-1	70	µM^-1min^-1	25°C	6.8	M161V	NADH	10,869,170
InhA	Mycobacterium tuberculosis	NADH	332	µM	700	min^-1			25°C	6.8	M161V	HXDCoA	10,869,170
InhA	Mycobacterium tuberculosis	DDCoA	72	µM	861	min^-1	11.9	µM^-1min^-1	25°C	6.8	A124V	NADH	10,869,170
InhA	Mycobacterium tuberculosis	NADH	142	µM	861	min^-1			25°C	6.8	A124V	DDCoA	10,869,170
InhA	Mycobacterium tuberculosis	DDCoA	null						25°C	6.8	Wild type	NADH, Triclosan 0.22 µM	10,869,170
InhA	Mycobacterium tuberculosis	NADH	null						25°C	6.8	Wild type	DDCoA, Triclosan 0.21 µM	10,869,170
InhA	Mycobacterium tuberculosis	DDCoA	null						25°C	6.8	Y158F	NADH, Triclosan 47 µM	10,869,170
InhA	Mycobacterium tuberculosis	DDCoA	null						25°C	6.8	S94A	NADH, Triclosan 0.14 µM	10,869,170
InhA	Mycobacterium tuberculosis	NADH	null						25°C	6.8	I47T	DDCoA, Triclosan 0.18 µM	10,869,170
InhA	Mycobacterium tuberculosis	NADH	null						25°C	6.8	I21V	DDCoA, Triclosan 0.12 µM	10,869,170
InhA	Mycobacterium tuberculosis	HXDCoA	null						25°C	6.8	M161V	NADH, Triclosan 4.3 µM	10,869,170
CelB	Pyrococcus furiosus	pNp-gal	1.11	mM	90	s^-1	81.1	mM^-1s^-1	30°C	5.4			10,879,560
CelB	Pyrococcus furiosus	pNp-gal	1.31	mM	236	s^-1	180.2	mM^-1s^-1	40°C	5.4			10,879,560
CelB	Pyrococcus furiosus	pNp-gal	2.05	mM	580	s^-1	282.9	mM^-1s^-1	50°C	5.4			10,879,560
CelB	Pyrococcus furiosus	pNp-gal	2.09	mM	1222	s^-1	584.7	mM^-1s^-1	60°C	5.4			10,879,560
CelB	Pyrococcus furiosus	pNp-gal	1.97	mM	1486	s^-1	754.3	mM^-1s^-1	65°C	5.4			10,879,560
CelB	Pyrococcus furiosus	pNp-gal	2.8	mM	2907	s^-1	1038.2	mM^-1s^-1	75°C	5.4			10,879,560
CelB	Pyrococcus furiosus	pNp-gal	4.1	mM	4863	s^-1	1186.1	mM^-1s^-1	85°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-gal	0.59	mM	56	s^-1	94.9	mM^-1s^-1	30°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-gal	0.82	mM	143	s^-1	174.4	mM^-1s^-1	40°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-gal	1.09	mM	293	s^-1	268.8	mM^-1s^-1	50°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-gal	0.72	mM	648	s^-1	900.0	mM^-1s^-1	60°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-gal	0.77	mM	1000	s^-1	1298.7	mM^-1s^-1	65°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-gal	1.61	mM	2288	s^-1	1421.1	mM^-1s^-1	75°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-gal	3.16	mM	4380	s^-1	1386.1	mM^-1s^-1	85°C	5.4			10,879,560
CelB	Pyrococcus furiosus	pNp-gal	2.8	mM	2900	s^-1	1000	mM^-1s^-1	75°C	5.4			10,879,560
CelB	Pyrococcus furiosus	oNp-gal	2.57	mM	9600	s^-1	3700	mM^-1s^-1	75°C	5.4			10,879,560
CelB	Pyrococcus furiosus	pNp-glu	0.37	mM	2600	s^-1	7100	mM^-1s^-1	75°C	5.4			10,879,560
CelB	Pyrococcus furiosus	oNp-glu	0.25	mM	3300	s^-1	12900	mM^-1s^-1	75°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-gal	1.61	mM	2300	s^-1	1400	mM^-1s^-1	75°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	oNp-gal	0.87	mM	2400	s^-1	2700	mM^-1s^-1	75°C	5.4			10,879,560
Sßgly	Sulfolobus solfataricus	pNp-glu	0.026	mM	520	s^-1	20000	mM^-1s^-1	75°C	5.4		Lower substrate concentrations	10,879,560
Sßgly	Sulfolobus solfataricus	pNp-glu	1.32	mM	1000	s^-1	7600	mM^-1s^-1	75°C	5.4		Higher substrate concentrations	10,879,560
Sßgly	Sulfolobus solfataricus	oNp-glu	0.053	mM	702	s^-1	13250	mM^-1s^-1	75°C	5.4		Lower substrate concentrations	10,879,560
HMG-CoA reductase	Archaeoglobus fulgidus	HMG-CoA	1,752	μM	2.0	s^-1	1.14	mM^-1s^-1	50°C	6.0		NADPH	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	HMG-CoA	1,752	μM	2.0	s^-1	1.14	mM^-1s^-1	50°C	9.0		NADH	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	NADPH	500	μM	1.0	s^-1	2.0	mM^-1s^-1	50°C	6.0		HMG-CoA	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	NADH	160	μM	2.0	s^-1	12.5	mM^-1s^-1	50°C	9.0		HMG-CoA	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	Mevaldehyde	5,000	μM	0.40	s^-1	0.08	mM^-1s^-1	50°C	6.0		NADPH	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	Mevaldehyde	5,000	μM	5.0	s^-1	1.0	mM^-1s^-1	50°C	7.0		NADH	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	NADPH	540	μM	0.40	s^-1	0.74	mM^-1s^-1	50°C	6.0		Mevaldehyde	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	NADH	200	μM	5.0	s^-1	25	mM^-1s^-1	50°C	7.0		Mevaldehyde	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	Mevaldehyde	400	μM	0.05	s^-1	0.125	mM^-1s^-1	50°C	9.0		NADP^+	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	Mevaldehyde	400	μM	0.10	s^-1	0.25	mM^-1s^-1	50°C	10.0		NAD^+	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	CoASH	20	μM	0.05	s^-1	2.5	mM^-1s^-1	50°C	9.0		NADP^+	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	CoASH	20	μM	0.10	s^-1	5.0	mM^-1s^-1	50°C	10.0		NAD^+	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	NADP^+	1,100	μM	0.05	s^-1	0.045	mM^-1s^-1	50°C	9.0		Mevaldehyde, CoASH	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	NAD^+	2,100	μM	0.10	s^-1	0.048	mM^-1s^-1	50°C	10.0		Mevaldehyde, CoASH	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	Mevalonate	620	μM	0.25	s^-1	0.40	mM^-1s^-1	50°C	7.0		NADP^+	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	Mevalonate	620	μM	5.0	s^-1	8.1	mM^-1s^-1	50°C	10.0		NAD^+	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	CoASH	120	μM	0.25	s^-1	2.1	mM^-1s^-1	50°C	7.0		NADP^+	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	CoASH	30	μM	5.0	s^-1	167	mM^-1s^-1	50°C	10.0		NAD^+	10,892,815
HMG-CoA reductase	Archaeoglobus fulgidus	NADP^+	1,700	μM	0.25	s^-1	0.15	mM^-1s^-1	50°C	7.0		Mevalonate, CoASH	10,892,815
TruB	Escherichia coli	tRNA	148	nM	0.110	s^-1	7.4 × 10^5	M^-1s^-1	37°C	7.5	Wild-type		10,924,141