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PMC9429973
Notably, similar Ab levels were observed in those infected with Omicron and in non-infected pts, reflecting the genetic drift of this variant.
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PMC11001582
c,d, Representative confocal images of immunofluorescence staining showing absence of colocalization between the astrocytic markers S100β (c) or GFAP (d) (yellow) and HA-tag (red) in (GR)400 mouse brain cortex (c) and lumbar spinal cord ventral horn (d) at 6 months of age, n = 5 mice per genotype.
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PMC10204952
The experimental data were analyzed by one-way ANOVA for each individual type of cell culture media series and two-way ANOVA followed by Tukey’s post-hoc test on three types of cell culture media series.
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PMC11397654
The formation of a self-inclusion complex (i.e., intramolecular host–guest conjugate), rather than intermolecular polymerisation, was confirmed by NMR spectroscopy and trapped ion mobility spectrometry−mass spectrometry (TIMS–MS).
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PMC11750712
Lymph node ddPCR AUTO4 copy number ranged from 111 to 171,700 VCN per μg DNA.
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PMC11551844
Gold nanoparticles (AuNPs) exhibit different physical properties compared to small molecules, bulk materials and other nanoparticles.
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PMC11478724
This was done to identify possible miRNA targets on VDAC1 and STAT3.
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PMC10652261
HRMS (m/z): [M + H] calcd for C24H25ClN4S: 437.1561.
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PMC8345486
Spheroid disintegration under the influence of cisplatin was mainly observed in the A2780 line and its sublines.
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PMC10745221
These results indicate that no observable changes in protein arginine methylation accompany the reversion from the methionine-addicted and metastatic phenotype of the parent cells.
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PMC9429973
40% were male 60% female, the median age was 61 yrs. (
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PMC11098378
After cultures reached an OD600 of 0.4–0.5, protein expression was induced with 0.2 mM IPTG overnight at 18°C.
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PMC11678841
In this in vitro study, we assessed for the first time the potential of α-TOH and the vitamin E metabolite GA in modulating the epithelial immune response to C. albicans infection.
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PMC11694346
These advantages include tunable optical properties, fast response times, high sensitivity (even at micromolar concentrations), cost-effectiveness and straightforward operational procedures.
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PMC8751435
Briefly, cells were seeded in 96‐well plates at a density of 1 × 10 cells/well and treated with different concentrations of CME (0, 1, 10, 100, 200, 400, 600, 800, and 1000 μg/ml) for 24, 48, or 72 h, and cells were observed by using microscope before addition of CCK‐8.
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PMC11637820
Interestingly, our study revealed gram-positive bacteria to be more sensitive to CAP than gram-negative microbes.
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PMC10669966
Antigens were detected with SuperSignal™ West Pico PLUS chemiluminescent substrate (Cat.
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PMC11718274
Meanwhile, exposure of A-431 cancer cells to Y2O3NPs also significantly elevated the expression level of apoptotic p53 and mitochondrial ND3 genes compared to the elevations induced by doxorubicin treatment as depicted in Table 5.
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PMC8409415
The identity of each cell line was confirmed by DNA fingerprinting using short tandem repeat profiling., ,
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PMC10965680
In conclusion, for the first time, a comparative analysis of the Xiangdong black goat genome with those of other goat populations in China revealed genetic diversity, population genetic structure, and selection signals related to environmental adaptation-specific traits.
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PMC11489351
EIPA, 5-(N-ethyl-N-isopropyl) amiloride; NH4Cl, ammonium chloride; pHi, intracellular pH. In addition to these pronounced pH-dependent changes in glycolytic enzyme expression, our RNA-Seq analysis also revealed pHi-dependent transcription of several enzymes regulating pyruvate fate (Fig. 5B).
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PMC9429973
Integrative analysis revealed 118 proteins that were differentially expressed and/or precipitated by GATA1-IP, of which 49 were enriched in malignant and 69 proteins in normal erythroblasts (q<0.5).
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PMC11792090
Blood samples were collected 8 days post infusion.
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PMC11535726
In addition, the comparison of the nucleotides between the sequences of K.bulbosapicalis and K.dujuanhuensis showed differences of 9% (47/512 bp) across ITS, 1% (8/812 bp) across LSU and 0.1% (2/1003 bp) across SSU, excluding gaps.
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PMC11779876
Comprehensive clonality profiling will therefore be mandatory for effective allocation of these complex cellular therapies.
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PMC11755624
Its overexpression suppresses the activity of PI3K/AKT/mTOR in TNF-stimulated keratinocytes, which was associated with suppressed inflammation .
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PMC11216402
The median CV AUROC is 0.925.
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PMC11291697
In fact, altered glycine and D-serine levels have been related to the development of kindling epileptogenesis in mice .
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PMC11763111
E The mean value of average radiance in each group of mice was monitored using bioluminescence in order to compare the therapeutic efficacy of 4KO-LLT1 CAR-T with that of other CAR-T cells.
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PMC8357004
Other modifications include histone deacetylation affect local compaction of chromatin structure leads to TSGs silencing.
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PMC11011837
By digging into the molecular phenotype of these cell lines, we aim to identify potential variations among them, possibly allowing for the classification and stratification of the different cell lines at the metabolic level, unraveling differences, or similarities, in the same genetic subgroup.
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PMC11795610
It has been shown that myricetin significantly reduces the secretion of pro-inflammatory cytokines, such as IL-1β and IL-10, and has inhibitory effect on inflammation induced by LPS.
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PMC11711663
Clinical data were extracted from the MD Anderson Cancer Center’s custom electronic medical record system.
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PMC11538390
Allele frequency was estimated using birthplace data of 306,678 FinnGen study participants.
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PMC11566417
The inoculum was aliquoted into four wells of a 96-well plate at 200 µL/well for a concentration of 5000 CFU/well.
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PMC9429973
Forced SETD2 re-expression was obtained by nucleofection: 10 HMC-1.2 cells were resuspended in 85μl of cell line Nucleofector Solution V and 2μg of a SETD2 (GFP-tagged) construct were added.
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PMC11055443
Thus, inhibition of metalloproteases by TIMPs inhibits the access and attachment of bacteria to the Caco-2 cells.
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PMC11672617
The biological activity of the synthesized compounds was evaluated using four cell lines: Jurkat, K562, A549, and HEK293.
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PMC11621493
After the incubation, cells were washed two times with 1 ml PBS, and cellular lipids were extracted directly from the 6-well plates using 1 ml hexane/isopropanol (v/v, 3:2) for 10 min, followed by 0.5 ml hexane/isopropanol (v/v, 3:2) for 5 min and 0.5 ml 100% isopropanol for 5 min.
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PMC7185206
Additional flow diagrams in Supplemental Figure 8.
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PMC7185206
To determine whether PD-L1 protein levels are commensurate with CD274 (PD-L1) mRNA expression in human tumors, we queried The Cancer Genome Atlas (TCGA) for normalized reverse phase protein array (RPPA) PD-L1 protein levels and for normalized CD274 RNA-seq transcript sequences per million mapped fragments (FPKM) and calculated a PD-L1 protein-to-mRNA ratio for each sample.
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PMC9429973
Summary/Conclusion: Esomeprazole 40mg twice daily resulted in a significant reduction in liver iron content when compared to placebo in a heterogeneous group of patients with non-transfusion-dependent hereditary anemias with mild-to-moderate iron overload.
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PMC11345828
Incubation of sample and beads was performed as suggested at 4 °C overnight (16 h) in the dark, incubation with Biotinylated Detection Antibody was performed for 1 h at room temperature with shaking in the dark.
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PMC11348300
To better account for tumor heterogeneity, larger tumors were divided into sections before imaging, and the resulting signals were averaged during analysis.
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PMC11627060
This reduction in surface charge may be attributed to alterations in the secondary structure of the NPs, leading to decreased surface charges during NP assembly.
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PMC6684588
Plasmids were stably transfected in Chinese hamster ovary (CHO) DUKX cells and used to express ACE-083 (FST291-Fc) and FST315-Fc.
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PMC11594588
G protein-coupled receptors (GPCRs) are the most numerous and highly diverse group of membrane proteins responsible for transmitting and mediating external signals and stimuli across the lipid bilayer to effector sites located within the cell .
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PMC11746948
The samples were then subjected to methanol-chloroform-H2O precipitation, as described above, and resuspended in 30 µL lysis buffer, warmed to 37 °C for 10 min, and briefly (∼5 s) sonicated and treated with 90 µL lysis buffer with 0.2% Triton X-100 and 1.33 mM mPEG-Mal (5 kDa; Sigma) for a final concentration of 1 mM mPEG-Mal.
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PMC11723947
Unless otherwise stated, Th1 culture medium also contained 10 ng/mL rmIL-12 (R&D Systems) and 10 μg/mL anti-IL-4 antibody (11B11, BioXcell), and Th17 culture medium contained 10 ng/mL rmIL-1β (R&D Systems), 20 ng/mL rmIL-6 (R&D Sytems), 1 ng/mL rhTGF-β 1 and 10 μg/mL anti-IL-4 and anti-IFN-γ (XMG1.2, BioXcell) antibodies.
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PMC10850553
The tag count of C5AR1 from acquired data sets is shown.
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PMC11750712
In phase 1 dose escalation, four dose levels were explored: cohort 1 (n = 3 patients) received 25 × 10 AUTO4 T cells; cohort 2 (n = 2 patients) received 75 × 10 AUTO4 T cells; cohort 3 (n = 1 patient) received 225 × 10 AUTO4 T cells; and cohort 4 (n = 4 patients) received 450 × 10 AUTO4 T cells.
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PMC9261184
However, methane emissions over this voyage were 60% higher than those assumed by Roman-White et al., which can be attributed to the difference in the assumed methane slip.
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PMC10440586
Only 30% of patients with gliomas survive at five years after diagnosis, and this number drops to 6.8% for glioblastoma (GBM) .
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PMC11525028
Scale bars: 50 μm (left), 25 μm (right).Data are presented as the mean ± SD.
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PMC9105697
In 1 of the 2 patients with primary tumors, and 5 of the 13 samples with metastatic disease, breakpoint ctDNA was identified (Figure 2A).
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PMC11721587
However, unlike CC-Fu, CC-Fu could still phosphorylate its own activation loop as determined by the phospho-specific antibody pT158/S159 (fig. S1G) (26), as well as the coexpressed Cos2 and Sufu as indicated by their mobility shift (fig. S1H), suggesting that CC-Fu is catalytically active.
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PMC9429973
2018;17:1973).
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PMC5363531
Single and combined treatments were performed for 72 hours, sequential administrations at 72 (dasatinib pretreatment) + 72 hours (PEM incubation).
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PMC9512971
The length of the double-blind period (up to 14 days) was chosen because of the typical clinical course .
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PMC9429973
Results: A total of 215 patients were recruited.
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PMC10719213
The effect of cladribine on cell proliferation in Jurkat with 12 days (n = 5) and 16 days in culture (n = 5). (
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PMC11661040
Osteosarcoma, the most common primary bone malignancy, is a type of tumor originating from undifferentiated bone fibrous tissue, usually occurring in the distal femur and proximal tibia(Ritter et al. 2010).
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PMC11755519
The cargo they carry, such as RNA, DNA, proteins, metabolites, or lipids can affect the properties of target cells.
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PMC11747885
Compounds 41, 42, and 46 were excluded from this experiment because of their inherent cytotoxicity to HEK293 cells.
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PMC6114978
No angiosarcomas or chondrosarcomas were noted to exhibit loss of the Hippo kinases, but the sample sizes of these sarcomas were small.
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PMC6024716
Additionally, the role of OAZ1 in polyamine metabolism has been well studied , making it a promising target to explore the connection between OAZ1 inhibition and the level of different polyamines.
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PMC9436459
The other seven type D-I MDAAs with the diene moiety at C-6 of A ring have trans–trans and cis–trans configurations, as exemplified by 98–99 and 100–104, respectively.
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PMC7171147
N = 2.
[ { "tags": [ "O", "O", "O", "O" ], "tokens": [ "N", "=", "2", "." ] } ]
PMC11703896
Using RMSF simulation analysis, rapamycin was found to be more stable in ALK, EGFR and MET than other receptors.
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PMC11295144
To test whether the observed potentiation of NL2 effects by GABAARs may be a consequence of increased expression of NL2, we have transfected the HA-tagged NL2 cDNA into the control, α4β3δ-GABAAR- or α2β2γ2-GABAAR-expressing HEK293 cells and carried out ELISA using an HA tag-specific antibody (Supplementary Figure S3).
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PMC5311252
The oncomotif-miRNA signature was defined by selecting all genes with a false discovery rate<1.0 E-05 and an absolute log2 fold-change >1.
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PMC11746942
p values are indicated for each comparison.
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PMC9587866
These responses can effectively be dealt by using nanotechnological delivery methods to avert the side effects and further improve the effectiveness of the compounds (Patra et al., 2018).
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PMC5839394
SKBR3 cells were plated in 96 well plates at a density of 5 × 10/well, allowed to growth overnight and incubated for 24 h with different AgNPs-EPSs concentrations.
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PMC11194678
On the other hand, although the RNA-binding-deficient STAU1 HKΔRBD4 mutant possessed the LLPS ability, its failure to recruit and enrich target RNA resulted in a negligible impact on mTOR signaling and autophagy pathway, suggesting that both the LLPS and RNA-binding properties of STAU1 are required for its function in regulating RNA translation.
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PMC5034105
Moreover, vector-free JEV transmission due to persistence of virus in tonsils and virus shedding in oronasal secretions of pigs has recently been reported.
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PMC11635087
Notably, many algal cells become inactive at temperatures below 37℃, which is the optimal temperature for cultured mammalian cells, but schyzon cells remain active at this temperature, making it useful for synthetic biology research.
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PMC10976516
Lung cancer therapy relies on the tumor stage at the time of diagnosis (Table 1, types of treatment for various stages).
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PMC9964635
The protein c-kit tyrosine kinase is expected to interact strongly with cancer-targeted therapeutics.
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PMC11767793
Polyethyleneimine (PEI), also a polycation, is highly water soluble and is routinely used in gene transfection/delivery applications .
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PMC7723249
No difference in IL-8 production between CCHFV- and HAZV-infection was observed.
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PMC10993311
T2 = 20-26 weeks.
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PMC2118063
PE–Cy7 and A647-conjugated isotype-matched mAbs were used as controls.
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PMC10926885
By bypassing immunological checkpoints, which activate immune cells and cause them to attack cancer cells, several immunotherapy treatments for cancer are made possible.
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PMC11754784
Cancer cells were stained with CellTrace™ Far Red dye and M1 macrophages were stained with CellTrace™ CFSE for optical differentiation.
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PMC3035438
These results indicate that the purified chimeric IgG is highly specific to the target antigen but exhibits less reactivity in the initial culture supernatants due to interference by the excess of coexisting chicken IgM. Figure 3.Selection of antigen-specific chimeric IgG. (A) Primary screen of anti-apoferritin mAbs selected with the ADLib system from the chimera CX13 derived library.
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PMC11267830
Moreover, detailed histological analyses of liver and spleen samples confirmed reduced infiltration of RBM39-suppressed J.gamma1 cells in these organs (Fig. 5h).
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PMC11670407
However, the role of OTULIN in OS, especially in chemotherapy-resistant OS, has not been well investigated.
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PMC10142392
Accumulation at tumor sites depends on the camouflage, stability, blood circulation/retention, targeting ability of the nanoparticles, and other factors such as vascularization and angiogenesis .
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PMC8270637
Western blot was used to detect the expression of total NFATc1 protein and phosphorylated NFATc1 protein in T cells. (
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PMC11705067
Next, focusing on the functional analysis of the differentially expressed genes that are positively and negatively associated with both FDX1 and DLAT genes in ccRCC.
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PMC11001582
n (independent biological replicates, one eye counted per fly) = 15 WT/+, 15 WT/Mp RNAi, 12 GR36/+, 10 GR36/Mp RNAi; two-way ANOVA, Bonferroni’s multiple comparison.
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PMC3164356
Chromosomes were diluted and loaded onto the microfluidic chip so that around half of the 48 chambers were occupied by single chromosomes.
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PMC11349530
Kynurenine has been demonstrated to promote the expression of the protective TGF-β, the differentiation of Treg cells, and the induction of IDO1 expression in dendritic cells (DCs) (89–91).
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PMC4360730
Terms from PRO can be used to annotate biosample treatments that use protein reagents.
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PMC7762347
Despite the fact that humans and animals are in their everyday life exposed to complex cyanobacterial mixtures, the studies describing the combined effects of these toxins are scarce.
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PMC11673128
Cell suspension of 500.000 AS4 cells, previously exposed to ALKi, and 250.000 macrophages were co-cultured with anti-CD47 mAb at a concentration of 10 μg/mL and incubated in a serum-free IMDM within a 4-well Nunc™ Lab-Tek™ II Chamber Slide™ System (Thermofisher) for 1–2 hours, in a humidified 5% CO2 incubator at 37 °C.
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PMC11285179
Scale bar = 100 μm.
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PMC9429973
Methods: 32 blood samples were collected from 15 ERCC6L2 patients (some in multiple time points), and 6 healthy controls.
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PMC10154881
Mice were implanted subcutaneously with B16F10N4 melanoma and intratumorally treated saline (MOCK) or rMV‐SLAMblind (rMV) at day 4, day 5, day 6, day 8, and day 10.
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