Datasets:

EMBO
/

SourceData

<article doi="10.1038/35022604" abstract=""Lysosomal glycogen storage disease with normal acid maltase" which was originally described by Danon et al., is characterized clinically by cardiomyopathy, myopathy and variable mental retardation. The pathological hallmark of the disease is intracytoplasmic vacuoles containing autophagic material and glycogen in skeletal and cardiac muscle cells. Sarcolemmal proteins and basal lamina are associated with the vacuolar membranes. Here we report ten unrelated patients, including one of the patients from the original case report, who have primary deficiencies of LAMP-2, a principal lysosomal membrane protein. From these results and the finding that LAMP-2-deficient mice manifest a similar vacuolar cardioskeletal myopathy, we conclude that primary LAMP-2 deficiency is the cause of Danon disease. To our knowledge this is the first example of human cardiopathy-myopathy that is caused by mutations in a lysosomal structural protein rather than an enzymatic protein."><fig id="2628"><title></title><label>figf1</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2628"/><sd-panel panel_id="9110">a, The 2-bp deletion identified in exon 9b of patient 1. b, The T-to-A nonsense mutation in exon 4 of patient 2 (stop codon is underlined). c, Upper panel, intronic mutation in patient 3; lower panel, <sd-tag id="sdTag3" source="sdapi" category="assay" entity_type="" role="" text="sequencing" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">sequencing</sd-tag> of the <sd-tag id="sdTag1" source="sdapi" category="assay" entity_type="" role="" text="RT–PCR" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RT-PCR</sd-tag> product in patient 3, showing exon-6 skipping. d, Upper panel, The G-to-A point mutation at the splice-donor site in intron 5 of patient 6; lower panel, <sd-tag id="sdTag2" source="sdapi" category="assay" entity_type="" role="" text="sequencing" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">sequencing</sd-tag> of the <sd-tag id="sdTag4" source="sdapi" category="assay" entity_type="" role="" text="RT–PCR" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RT-PCR</sd-tag> product in patient 6, showing a 6-bp insertion at the junction between exons 5 and 6. The inserted sequence (underlined) is derived from the first six nucleotides of intron 5 (underlined), which creates a stop codon (dotted). Most probably, nucleotides 7 and 8, GT (double underlined), in intron 5 are alternatively recognized as a splice-donor site. e, Upper panel, the 10-bp deletion in patient 9. This mutation deletes four nucleotides from intron 5 and six from exon 6. Lower panel, sequence of the <sd-tag id="sdTag5" source="sdapi" category="assay" entity_type="" role="" text="RT–PCR" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RT-PCR</sd-tag> product in patient 9 reveals the deletion of the 10 nucleotides at the 5′ end of exon 6. The 'AG' (double underlined) in exon 6 immediately after the genomic DNA deletion appears to be recognized as an alternative splice-acceptor site.<graphic href="https://api.sourcedata.io/file.php?panel_id=9110"/></sd-panel></fig><fig id="2629"><title></title><label>figf2</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2629"/><sd-panel panel_id="9111">Figure 2 Representation of the mutant <sd-tag id="sdTag53" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2b" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2b</sd-tag>. 'Normal' represents the <sd-tag id="sdTag54" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2b" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2b</sd-tag> amino-acid sequence. Because patient 1, who developed full DD symptoms, has a mutation in exon 9b, the <sd-tag id="sdTag55" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2b" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2b</sd-tag> isoform is most probably responsible for DD. Residue 265, one of the putative disulphide-bond-creating cysteines, and two glycosylation sites are lost by exon-6 skipping in patient 3, which probably results in a significant change in the secondary structure. Black boxes represent the loops created between two cystein residues flanking each of the four loop regions; Y depicts a glycosylation site; hatched boxes show altered amino acids caused by frameshift mutations; dashes represent missing amino acids due to exon-6 skipping in patient 3. All of the other mutant proteins lack the transmembrane domain (grey bar) because of nonsense or frameshift mutations. Thus, those molecules cannot function as lysosomal 'membrane' proteins.<graphic href="https://api.sourcedata.io/file.php?panel_id=9111"/></sd-panel></fig><fig id="2630"><title></title><label>figf3</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2630"/><sd-panel panel_id="9112">a, <sd-tag id="sdTag8" source="sdapi" category="entity" entity_type="tissue" role="experiment" text="Skeletal muscle" ext_ids="UBERON:0001134" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="skeletal muscle tissue" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">Skeletal muscle</sd-tag>. Muscle extracts from <sd-tag id="sdTag9" source="sdapi" category="entity" entity_type="organism" role="component" text="patients" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patients</sd-tag> 1-6 and 10 and a control were <sd-tag id="sdTag10" source="sdapi" category="assay" entity_type="" role="" text="blotted" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">blotted</sd-tag> and labelled with antibodies against <sd-tag id="sdTag11" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> (top panel) and <sd-tag id="sdTag12" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="β-actin" ext_ids="P60709" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="ACTB" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">β-actin</sd-tag> (bottom panel). <sd-tag id="sdTag17" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> is deficient in all muscles examined except the sample from patient 1 that showed a trace amount of protein, which is probably the <sd-tag id="sdTag13" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2a" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2a</sd-tag> isoform. In support of this idea, <sd-tag id="sdTag14" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2b" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2b</sd-tag> is the principal <sd-tag id="sdTag18" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> species in skeletal muscle6. Numbers denote <sd-tag id="sdTag19" source="sdapi" category="entity" entity_type="organism" role="component" text="patients" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patients</sd-tag> as in Table 1; C, control. b, <sd-tag id="sdTag15" source="sdapi" category="entity" entity_type="tissue" role="experiment" text="Heart" ext_ids="UBERON:0000948" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="heart" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">Heart</sd-tag>. Protein was extracted from <sd-tag id="sdTag16" source="sdapi" category="entity" entity_type="tissue" role="experiment" text="cardiac muscle" ext_ids="UBERON:0001133" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="cardiac muscle tissue" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">cardiac muscle</sd-tag> of patient 4 and a control, <sd-tag id="sdTag20" source="sdapi" category="assay" entity_type="" role="" text="blotted" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">blotted</sd-tag> onto a nitrocellulose membrane, and labelled with the antibodies against <sd-tag id="sdTag21" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> (too panel) and <sd-tag id="sdTag22" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="β-actin" ext_ids="P60709" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="ACTB" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">β-actin</sd-tag> (bottom panel). <sd-tag id="sdTag23" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> is deficient also in <sd-tag id="sdTag24" source="sdapi" category="entity" entity_type="tissue" role="experiment" text="cardiac muscle" ext_ids="UBERON:0001133" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="cardiac muscle tissue" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">cardiac muscle</sd-tag>. C, control; 4, patient 4.<graphic href="https://api.sourcedata.io/file.php?panel_id=9112"/></sd-panel></fig><fig id="2631"><title></title><label>figf4</label><graphic href="https://api.sourcedata.io/file.php?figure_id=2631"/><sd-panel panel_id="9113"><sd-tag id="sdTag25" source="sdapi" category="assay" entity_type="" role="" text="Serial transverse sections" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Serial transverse sections</sd-tag> of the <sd-tag id="sdTag26" source="sdapi" category="assay" entity_type="" role="" text="biopsied" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">biopsied</sd-tag><sd-tag id="sdTag27" source="sdapi" category="entity" entity_type="tissue" role="assayed" text="muscle" ext_ids="UBERON:0002385" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="muscle tissue" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">muscle</sd-tag> from six DD <sd-tag id="sdTag36" source="sdapi" category="entity" entity_type="organism" role="component" text="patients" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patients</sd-tag>, and samples from a XMEA patient and a normal control, were <sd-tag id="sdTag28" source="sdapi" category="assay" entity_type="" role="" text="immunostained" ext_ids="BAO_0002426" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunofluorescent labeling" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunostained</sd-tag> with antibodies against <sd-tag id="sdTag37" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> and <sd-tag id="sdTag29" source="sdapi" category="entity" entity_type="protein" role="assayed" text="limp-I" ext_ids="Q14108" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="SCARB2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">limp-I</sd-tag>. <sd-tag id="sdTag38" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> is absent in all DD muscles (patient 6 shown here), whereas it is present in the XMEA <sd-tag id="sdTag39" source="sdapi" category="entity" entity_type="tissue" role="assayed" text="muscle" ext_ids="UBERON:0002385" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="muscle tissue" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">muscle</sd-tag>. The antibody against <sd-tag id="sdTag40" source="sdapi" category="entity" entity_type="protein" role="assayed" text="limp-I" ext_ids="Q14108" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="SCARB2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">limp-I</sd-tag> highlighted <sd-tag id="sdTag30" source="sdapi" category="entity" entity_type="subcellular" role="component" text="vacuoles" ext_ids="GO:0005773" norm_text="" ext_dbs="Gene Ontology" in_caption="True" ext_names="vacuole" ext_tax_ids="" ext_tax_names="" ext_urls="http://amigo.geneontology.org/amigo/term/">vacuoles</sd-tag> in both DD and XMEA muscles. <sd-tag id="sdTag31" source="sdapi" category="entity" entity_type="protein" role="component" text="LAMP-1" ext_ids="P11279" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-1</sd-tag> antibody faintly stained both DD and XMEA muscles, suggesting that the expression level of <sd-tag id="sdTag41" source="sdapi" category="entity" entity_type="protein" role="component" text="LAMP-1" ext_ids="P11279" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-1</sd-tag> might be lower in skeletal <sd-tag id="sdTag42" source="sdapi" category="entity" entity_type="tissue" role="assayed" text="muscle" ext_ids="UBERON:0002385" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="muscle tissue" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">muscle</sd-tag> than <sd-tag id="sdTag43" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> or <sd-tag id="sdTag44" source="sdapi" category="entity" entity_type="protein" role="assayed" text="limp-I" ext_ids="Q14108" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="SCARB2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">limp-I</sd-tag> (data not shown). The <sd-tag id="sdTag45" source="sdapi" category="entity" entity_type="tissue" role="assayed" text="muscle" ext_ids="UBERON:0002385" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="muscle tissue" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">muscle</sd-tag> from the patient without a lamp-2 mutation also showed <sd-tag id="sdTag46" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> deficiency on <sd-tag id="sdTag32" source="sdapi" category="assay" entity_type="" role="" text="immunohistochemistry" ext_ids="BAO_0000415" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunohistochemistry" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunohistochemistry</sd-tag> similar to genetically proven DD <sd-tag id="sdTag47" source="sdapi" category="entity" entity_type="organism" role="component" text="patients" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patients</sd-tag> with primary <sd-tag id="sdTag48" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag> deficiency (data not shown). The <sd-tag id="sdTag33" source="sdapi" category="assay" entity_type="" role="" text="immunohistochemical stains" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">immunohistochemical stains</sd-tag> of <sd-tag id="sdTag49" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LAMP-2" ext_ids="P13473" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-2</sd-tag>, <sd-tag id="sdTag50" source="sdapi" category="entity" entity_type="protein" role="assayed" text="limp-I" ext_ids="Q14108" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="SCARB2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">limp-I</sd-tag>, and <sd-tag id="sdTag51" source="sdapi" category="entity" entity_type="protein" role="component" text="LAMP-1" ext_ids="P11279" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="LAMP1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LAMP-1</sd-tag> in normal <sd-tag id="sdTag52" source="sdapi" category="entity" entity_type="tissue" role="assayed" text="muscle" ext_ids="UBERON:0002385" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="muscle tissue" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">muscle</sd-tag> shows numerous tiny particles and accentuates <sd-tag id="sdTag34" source="sdapi" category="entity" entity_type="tissue" role="component" text="type 1 fibres" ext_ids="UBERON:0004057" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="skeletal muscle fiber triad" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">type 1 fibres</sd-tag>. H&amp;amp;E, <sd-tag id="sdTag35" source="sdapi" category="assay" entity_type="" role="" text="haematoxylin and eosin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">haematoxylin and eosin</sd-tag>, original magnification ×150.<graphic href="https://api.sourcedata.io/file.php?panel_id=9113"/></sd-panel></fig></article>