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<article doi="10.1002/emmm.201100193" abstract="Cancer cells are metabolically stressed during tumour progression due to limited tumour vascularity and resultant nutrient, growth factor and oxygen deficiency that can induce cell death and inhibit tumour growth. We demonstrate that Rab25, a small GTPase involved in endosomal recycling, that is genomically amplified in multiple tumour lineages, is a key regulator of cellular bioenergetics and autophagy. RAB25 enhanced survival during nutrient stress by preventing apoptosis and autophagy via binding and activating AKT leading to increased glucose uptake and improved cellular bioenergetics. Unexpectedly, Rab25 induced the accumulation of glycogen in epithelial cancer cells, a process not previously identified. Strikingly, an increase in basal ATP levels combined with AKT-dependent increases in glucose uptake and glycogen storage allowed maintenance of ATP levels during bioenergetic stress. The clinical relevance of these findings was validated by the ability of a Rab25-dependent expression profile enriched for bioenergetics targets to identify patients with a poor prognosis. Thus, Rab25 is an unexpected regulator of cellular bioenergetics implicated as a useful biomarker and potential therapeutic target."><fig id="1310"><title></title><label>Figure 1</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1310"/><sd-panel panel_id="3058">A. <sd-tag id="sdTag20" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression decreases <sd-tag id="sdTag528" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="nutrient" ext_ids="CHEBI:33284" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="nutrient" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">nutrient</sd-tag> withdrawal (24 h) induced cell death in ovarian <sd-tag id="sdTag1" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag> and <sd-tag id="sdTag2" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="IOSE29" ext_ids="CVCL_5535" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="IOSE-29" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">IOSE29</sd-tag> cells measured using the <sd-tag id="sdTag486" source="sdapi" category="assay" entity_type="" role="" text="Cell death ELISA plus assay" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Cell death ELISA plus assay</sd-tag> (left); cells were cultured in complete media (containing 5% <sd-tag id="sdTag4" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag>), <sd-tag id="sdTag529" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> free media (SF), complete media plus <sd-tag id="sdTag6" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="2DG" ext_ids="CHEBI:15866" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="2-deoxy-D-glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">2DG</sd-tag>, <sd-tag id="sdTag530" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> and <sd-tag id="sdTag7" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> free media (SF-glu), or in <sd-tag id="sdTag8" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="amino acid" ext_ids="CHEBI:33709" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="amino acid" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">amino acid</sd-tag>, <sd-tag id="sdTag14" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and SF <sd-tag id="sdTag485" source="sdapi" category="entity" entity_type="undefined" role="component" text="Earls buffer salt solution" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Earls buffer salt solution</sd-tag> (<sd-tag id="sdTag9" source="sdapi" category="entity" entity_type="undefined" role="component" text="EBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">EBSS</sd-tag>). (a) p < 0.01 versus control and (b) p < 0.05 versus pcDNA. (Right) Percentage of apoptotic cells (sub-G0 population) by <sd-tag id="sdTag10" source="sdapi" category="assay" entity_type="" role="" text="flow cytometry" ext_ids="BAO_0000005" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="flow cytometry" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">flow cytometry</sd-tag> in <sd-tag id="sdTag15" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag> cells following <sd-tag id="sdTag16" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag531" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> withdrawal (Glu/SF). (a) p < 0.001 versus <sd-tag id="sdTag17" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag>pcDNA control and (b) p < 0.05 <sd-tag id="sdTag18" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag><sd-tag id="sdTag21" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> versus <sd-tag id="sdTag19" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag>pcDNA control.<graphic href="https://api.sourcedata.io/file.php?panel_id=3058"/></sd-panel><sd-panel panel_id="3059">B. Decreasing <sd-tag id="sdTag24" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression increases sensitivity to <sd-tag id="sdTag527" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="nutrient" ext_ids="CHEBI:33284" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="nutrient" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">nutrient</sd-tag> stress induced cell death. Expression of <sd-tag id="sdTag25" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> was down-regulated by siRNA or shRNA specific to <sd-tag id="sdTag26" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=3059"/></sd-panel><sd-panel panel_id="3061">C. <sd-tag id="sdTag40" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> regulates autophagy activity in ovarian cancer cells under <sd-tag id="sdTag534" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> and <sd-tag id="sdTag42" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> deprivation conditions. <sd-tag id="sdTag51" source="sdapi" category="assay" entity_type="" role="" text="Western blot" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">Western blot</sd-tag> for the 16kD <sd-tag id="sdTag614" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9BXW4///Q9H492///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3C///MAP1LC3A///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>-II fragment, indicative of autophagy activity, in <sd-tag id="sdTag43" source="sdapi" category="entity" entity_type="cell_line" role="component" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag> cells after 4 and 6 h of <sd-tag id="sdTag535" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> and <sd-tag id="sdTag45" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> withdrawal.<graphic href="https://api.sourcedata.io/file.php?panel_id=3061"/></sd-panel><sd-panel panel_id="3062">D. <sd-tag id="sdTag59" source="sdapi" category="assay" entity_type="" role="" text="Electron microscopic analysis" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Electron microscopy</sd-tag> of autophagy in ovarian <sd-tag id="sdTag54" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag> cells after 4 and 24 h of <sd-tag id="sdTag613" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> and <sd-tag id="sdTag58" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> withdrawal (upper panel, low magnification). High magnification images of boxed areas with arrowheads depicting <sd-tag id="sdTag61" source="sdapi" category="entity" entity_type="subcellular" role="assayed" text="autophagic vacuoles" ext_ids="GO:0005776" norm_text="" ext_dbs="Gene Ontology" in_caption="True" ext_names="autophagic vacuole" ext_tax_ids="" ext_tax_names="" ext_urls="http://amigo.geneontology.org/amigo/term/">autophagic vacuoles</sd-tag> (lower panel, inset; N: <sd-tag id="sdTag62" source="sdapi" category="entity" entity_type="subcellular" role="assayed" text="nucleus" ext_ids="GO:0005634" norm_text="" ext_dbs="Gene Ontology" in_caption="True" ext_names="nucleus" ext_tax_ids="" ext_tax_names="" ext_urls="http://amigo.geneontology.org/amigo/term/">nucleus</sd-tag>). Average number of <sd-tag id="sdTag63" source="sdapi" category="entity" entity_type="subcellular" role="assayed" text="autophagosomes" ext_ids="GO:0005776///GO:0032010" norm_text="" ext_dbs="Gene Ontology///Gene Ontology" in_caption="True" ext_names="autophagic vacuole///phagolysosome" ext_tax_ids="" ext_tax_names="" ext_urls="http://amigo.geneontology.org/amigo/term////http://amigo.geneontology.org/amigo/term/">autophagosomes</sd-tag> per cell was calculated by counting the number of <sd-tag id="sdTag64" source="sdapi" category="entity" entity_type="subcellular" role="assayed" text="autophagosomes" ext_ids="GO:0005776///GO:0032010" norm_text="" ext_dbs="Gene Ontology///Gene Ontology" in_caption="True" ext_names="autophagic vacuole///phagolysosome" ext_tax_ids="" ext_tax_names="" ext_urls="http://amigo.geneontology.org/amigo/term////http://amigo.geneontology.org/amigo/term/">autophagosomes</sd-tag> in 16 individual cells from two-independent experiments.<graphic href="https://api.sourcedata.io/file.php?panel_id=3062"/></sd-panel><sd-panel panel_id="3063">E-H. Expression of <sd-tag id="sdTag65" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> decreases <sd-tag id="sdTag66" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag539" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> deprivation induced signalling activation. Protein expression was measured by <sd-tag id="sdTag73" source="sdapi" category="assay" entity_type="" role="" text="RPPA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RPPA</sd-tag> or <sd-tag id="sdTag540">WB</sd-tag> analysis.E. <sd-tag id="sdTag68" source="sdapi" category="assay" entity_type="" role="" text="RPPA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RPPA</sd-tag> detection of time-dependent activation of <sd-tag id="sdTag72" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AMPK" ext_ids="Q13131///Q9Y478///P54646" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="PRKAA1///PRKAB1///PRKAA2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AMPK</sd-tag> after <sd-tag id="sdTag541" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="nutrient" ext_ids="CHEBI:33284" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="nutrient" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">nutrient</sd-tag> withdrawal.<graphic href="https://api.sourcedata.io/file.php?panel_id=3063"/></sd-panel><sd-panel panel_id="3065">E-H. Expression of <sd-tag id="sdTag75" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> decreases <sd-tag id="sdTag76" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag521" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> deprivation induced signalling activation. Protein expression was measured by <sd-tag id="sdTag548">RPPA</sd-tag> or <sd-tag id="sdTag543" source="sdapi" category="assay" entity_type="" role="" text="WB" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">WB</sd-tag> analysis.F. <sd-tag id="sdTag544" source="sdapi" category="assay" entity_type="" role="" text="WB" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">WB</sd-tag> analysis of <sd-tag id="sdTag78" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AMPK" ext_ids="P54646///Q13131///Q9Y478" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="PRKAA2///PRKAA1///PRKAB1" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AMPK</sd-tag> and <sd-tag id="sdTag80" source="sdapi" category="entity" entity_type="protein" role="assayed" text="acetyl-CoA carboxylase" ext_ids="O00763///Q13085" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACB///ACACA" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">acetyl-CoA carboxylase</sd-tag> (<sd-tag id="sdTag86" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="Q13085///O00763" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACA///ACACB" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag>) phosphorylation in <sd-tag id="sdTag81" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="HEY" ext_ids="CVCL_0297" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HEY" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HEY</sd-tag> ovarian cancer cells (upper panel). <sd-tag id="sdTag545" source="sdapi" category="assay" entity_type="" role="" text="WB" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">WB</sd-tag> of phospho-<sd-tag id="sdTag85" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="Q13085///O00763" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACA///ACACB" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag> levels in <sd-tag id="sdTag79" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag>, <sd-tag id="sdTag82" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="IOSE80ht" ext_ids="CVCL_5546" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="IOSE-80" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">IOSE80ht</sd-tag> and <sd-tag id="sdTag83" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="SKOV3" ext_ids="CVCL_0532" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="SK-OV-3" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">SKOV3</sd-tag> ovarian cells after 1 h of <sd-tag id="sdTag546" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="nutrient" ext_ids="CHEBI:33284" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="nutrient" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">nutrient</sd-tag> withdrawal (lower panel).<graphic href="https://api.sourcedata.io/file.php?panel_id=3065"/></sd-panel><sd-panel panel_id="3066">E-H. Expression of <sd-tag id="sdTag91" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> decreases <sd-tag id="sdTag92" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag549" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> deprivation induced signalling activation. Protein expression was measured by <sd-tag id="sdTag90" source="sdapi" category="assay" entity_type="" role="" text="RPPA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RPPA</sd-tag> or <sd-tag id="sdTag550">WB</sd-tag> analysis.G. <sd-tag id="sdTag94" source="sdapi" category="assay" entity_type="" role="" text="RPPA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RPPA</sd-tag> detection of phosphorylation of <sd-tag id="sdTag95" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="Q13085///O00763" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACA///ACACB" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag> after withdrawal of <sd-tag id="sdTag551" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> and <sd-tag id="sdTag97" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=3066"/></sd-panel><sd-panel panel_id="3067">E-H. Expression of <sd-tag id="sdTag103" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> decreases <sd-tag id="sdTag104" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag554" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> deprivation induced signalling activation. Protein expression was measured by <sd-tag id="sdTag555">RPPA</sd-tag> or <sd-pretag id="sdPretag14473386131850" parent-tag-id="628">WB</sd-pretag> analysis.H. Effect of <sd-tag id="sdTag107" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> down-regulation on <sd-tag id="sdTag108" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AMPK" ext_ids="Q13131///Q9Y478///P54646" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="PRKAA1///PRKAB1///PRKAA2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AMPK</sd-tag> (left panel) and <sd-tag id="sdTag109" source="sdapi" category="entity" entity_type="protein" role="assayed" text="ACC" ext_ids="Q13085///O00763" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="ACACA///ACACB" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">ACC</sd-tag> (right panel) phosphorylation. Total cellular protein, isolated from <sd-tag id="sdTag110" source="sdapi" category="entity" entity_type="cell_line" role="component" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag>pcDNA and <sd-tag id="sdTag111" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expressing cells 72 h post-transfection with either non-target (NT) RNAi or <sd-tag id="sdTag112" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> specific RNAi, was separated by <sd-tag id="sdTag491" source="sdapi" category="assay" entity_type="" role="" text="polyacrylamide gel electrophoresis (PAGE)" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">polyacrylamide gel electrophoresis (PAGE)</sd-tag> followed by <sd-tag id="sdTag492" source="sdapi" category="assay" entity_type="" role="" text="WB" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">WB</sd-tag> analysis. (a) p < 0.001 versus NT RNAi control and (b) p < 0.001, pcDNA versus <sd-tag id="sdTag113" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=3067"/></sd-panel></fig><fig id="1311"><title></title><label>Figure 2</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1311"/><sd-panel panel_id="3068">Expression of <sd-tag id="sdTag116" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> increases endogenous <sd-tag id="sdTag134" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> level in ovarian cancer cells. *p < 0.001 <sd-tag id="sdTag117" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> versus pcDNA. The mean cellular concentration of <sd-tag id="sdTag135" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> per mg of protein (based on the standard curve generated using a known amount of <sd-tag id="sdTag136" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag>) is 2.36−10 moles/mg for <sd-tag id="sdTag133" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="IOSE29ht" ext_ids="CVCL_5535" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="IOSE-29" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">IOSE29ht</sd-tag>pcDNA cells, 6.21−10 moles/mg for <sd-tag id="sdTag137" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="IOSE29ht" ext_ids="CVCL_5535" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="IOSE-29" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">IOSE29ht</sd-tag><sd-tag id="sdTag118" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> cells, 8.14−11 moles/mg for <sd-tag id="sdTag119" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="IOSE80ht" ext_ids="CVCL_5546" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="IOSE-80" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">IOSE80ht</sd-tag>pcDNA cells, 1.05−10 moles/mg for <sd-tag id="sdTag120" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="IOSE80ht" ext_ids="CVCL_5546" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="IOSE-80" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">IOSE80ht</sd-tag><sd-tag id="sdTag121" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> cells, 2.85−10 moles/mg for <sd-tag id="sdTag122" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag>pcDNA cells, 3.81−10 moles/mg for <sd-tag id="sdTag123" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag><sd-tag id="sdTag124" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> cells, 7.23−10 moles/mg for <sd-tag id="sdTag125" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="HEY" ext_ids="CVCL_0297" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HEY" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HEY</sd-tag>pcDNA cells and 9.82−10 moles/mg for <sd-tag id="sdTag126" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="HEY" ext_ids="CVCL_0297" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HEY" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HEY</sd-tag><sd-tag id="sdTag127" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> cells.<graphic href="https://api.sourcedata.io/file.php?panel_id=3068"/></sd-panel><sd-panel panel_id="3069"><sd-tag id="sdTag138" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression increases <sd-tag id="sdTag139" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> synthesis and delays fall in total cellular <sd-tag id="sdTag140" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> levels after <sd-tag id="sdTag141" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag525" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> withdrawal, p < 0.001 <sd-tag id="sdTag143" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> versus control pcDNA.<graphic href="https://api.sourcedata.io/file.php?panel_id=3069"/></sd-panel><sd-panel panel_id="3070">Down-regulation of <sd-tag id="sdTag147" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression decreases ability to maintain <sd-tag id="sdTag148" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> after <sd-tag id="sdTag149" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag526" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> withdrawal (a) p < 0.05 SF-Glu versus <sd-tag id="sdTag151" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag> and (b) p < 0.05 versus control.<graphic href="https://api.sourcedata.io/file.php?panel_id=3070"/></sd-panel><sd-panel panel_id="3071">Expression of <sd-tag id="sdTag157" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> increases total cellular <sd-tag id="sdTag164" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> content while down-regulation of <sd-tag id="sdTag158" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression decreases total cellular <sd-tag id="sdTag165" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> content (µg of <sd-tag id="sdTag166" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag>/mg protein). Cells were cultured in complete media for 24 h before <sd-tag id="sdTag167" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> measurement. (a) p < 0.01 <sd-tag id="sdTag160" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> versus pcDNA, (b) p < 0.05 <sd-tag id="sdTag162" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> RNAi versus NT RNAi and (c) p < 0.05 shRAb25 versus shRNA control.<graphic href="https://api.sourcedata.io/file.php?panel_id=3071"/></sd-panel><sd-panel panel_id="3072">Decrease in cellular <sd-tag id="sdTag174" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> content after <sd-tag id="sdTag175" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag561" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag> withdrawal *p < 0.001 <sd-tag id="sdTag177" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> versus pcDNA.<graphic href="https://api.sourcedata.io/file.php?panel_id=3072"/></sd-panel><sd-panel panel_id="3073">Effect of <sd-tag id="sdTag569" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="GPi" ext_ids="16219026" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="BAY U6751 hydrate" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">GPi</sd-tag> (<sd-tag id="sdTag625" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Bay U6571" ext_ids="16219026" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="BAY U6751 hydrate" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">Bay U6571</sd-tag>) and <sd-tag id="sdTag190" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="oligomycin" ext_ids="CHEBI:25675" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="oligomycin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">oligomycin</sd-tag> on <sd-tag id="sdTag182" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> production. <sd-tag id="sdTag183" source="sdapi" category="entity" entity_type="cell_line" role="component" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag>pcDNA transfected and <sd-tag id="sdTag185" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expressing cells were pretreated with vehicle (control), 30 µM of <sd-tag id="sdTag193" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Bay U6571" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Bay U6571</sd-tag> (<sd-tag id="sdTag568" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="GPi" ext_ids="16219026" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="BAY U6751 hydrate" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">GPi</sd-tag>) or 25 µg/ml of <sd-tag id="sdTag195" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="oligomycin" ext_ids="CHEBI:25675" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="oligomycin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">oligomycin</sd-tag> for 2 h. Cells were then cultured in <sd-tag id="sdTag564" source="sdapi" category="entity" entity_type="undefined" role="component" text="serum" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">serum</sd-tag>- and <sd-tag id="sdTag187" source="sdapi" category="entity" entity_type="molecule" role="component" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> free <sd-tag id="sdTag624" source="sdapi" category="entity" entity_type="undefined" role="component" text="RPMI" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RPMI</sd-tag> 1640 in the presence or absence of <sd-tag id="sdTag643" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Bay U6571" ext_ids="16219026" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="BAY U6751 hydrate" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">Bay U6571</sd-tag> or <sd-tag id="sdTag197" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="oligomycin" ext_ids="CHEBI:25675" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="oligomycin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">oligomycin</sd-tag> for the indicated times. (a) p < 0.01 <sd-tag id="sdTag198" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="oligomycin" ext_ids="CHEBI:25675" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="oligomycin" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">oligomycin</sd-tag> time 0 versus control time 0 and (b) p < 0.01 versus time 0 at each treatment group.<graphic href="https://api.sourcedata.io/file.php?panel_id=3073"/></sd-panel><sd-panel panel_id="3074">Effect of <sd-tag id="sdTag199" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Bay U6571" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Bay U6571</sd-tag> on cellular <sd-tag id="sdTag200" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> (left panel) and <sd-tag id="sdTag201" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> (right panel) levels in <sd-tag id="sdTag202" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HEY" ext_ids="CVCL_0297" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HEY" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HEY</sd-tag> cells. (a) p < 0.01 versus pcDNA control time 0 at 5% <sd-tag id="sdTag204" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag> and (b) p < 0.01 <sd-tag id="sdTag570" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="GPi" ext_ids="16219026" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="BAY U6751 hydrate" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">GPi</sd-tag> versus control at each corresponding treatment.<graphic href="https://api.sourcedata.io/file.php?panel_id=3074"/></sd-panel><sd-panel panel_id="3075">Down regulation of <sd-tag id="sdTag211" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression and inhibition of <sd-tag id="sdTag212" source="sdapi" category="entity" entity_type="molecule" role="component" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> breakdown by <sd-tag id="sdTag573" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="GPi" ext_ids="16219026" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="BAY U6751 hydrate" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">GPi</sd-tag> administration increases <sd-tag id="sdTag214" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="2DG" ext_ids="CHEBI:15866" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="2-deoxy-D-glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">2DG</sd-tag> induced cell death *p < 0.05 versus shRNA control.<graphic href="https://api.sourcedata.io/file.php?panel_id=3075"/></sd-panel></fig><fig id="1312"><title></title><label>Figure 3</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1312"/><sd-panel panel_id="3076">A. <sd-tag id="sdTag222" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression increases phospho but not total <sd-tag id="sdTag224" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AKT" ext_ids="Q9Y243///P31749///P31751" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="AKT3///AKT1///AKT2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AKT</sd-tag> and <sd-tag id="sdTag225" source="sdapi" category="entity" entity_type="protein" role="assayed" text="GSK3" ext_ids="P49841///P49840" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="GSK3B///GSK3A" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">GSK3</sd-tag> levels in <sd-tag id="sdTag223" source="sdapi" category="entity" entity_type="cell_line" role="component" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag> cells leading to decreased phosphorylation of <sd-tag id="sdTag226" source="sdapi" category="entity" entity_type="protein" role="assayed" text="GS" ext_ids="P54840///P13807" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="GYS2///GYS1" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">GS</sd-tag> (pGS).<graphic href="https://api.sourcedata.io/file.php?panel_id=3076"/></sd-panel><sd-panel panel_id="3077">B. <sd-tag id="sdTag628" source="sdapi" category="assay" entity_type="" role="" text="WB" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">WB</sd-tag> analysis of <sd-tag id="sdTag236" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AKT" ext_ids="Q9Y243///P31749///P31751" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="AKT3///AKT1///AKT2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AKT</sd-tag> and <sd-tag id="sdTag237" source="sdapi" category="entity" entity_type="protein" role="assayed" text="GSK3" ext_ids="P49841///P49840" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="GSK3B///GSK3A" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">GSK3</sd-tag> phosphorylation confirming the inhibitory effect of PI3K pathways inhibitors <sd-tag id="sdTag241" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag>, <sd-tag id="sdTag574" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="GPi" ext_ids="16219026" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="BAY U6751 hydrate" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">GPi</sd-tag> and synthase kinase 3 inhibitor (<sd-tag id="sdTag242" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="GSK3i" ext_ids="176158" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="SB 216763" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">GSK3i</sd-tag>) in <sd-tag id="sdTag239" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HEY" ext_ids="CVCL_0297" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HEY" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HEY</sd-tag> cells.<graphic href="https://api.sourcedata.io/file.php?panel_id=3077"/></sd-panel><sd-panel panel_id="3078">C. Inhibition of <sd-tag id="sdTag249" source="sdapi" category="entity" entity_type="protein" role="intervention" text="GSK3" ext_ids="P49841///P49840" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="GSK3B///GSK3A" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">GSK3</sd-tag> activity increases total cellular <sd-tag id="sdTag250" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> levels. pcDNA transfected and <sd-tag id="sdTag252" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expressing ovarian cancer cells were cultured in complete media in the presence of 10 µM <sd-tag id="sdTag253" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="GSK3i" ext_ids="176158" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="SB 216763" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">GSK3i</sd-tag> for the indicated times. Total cellular <sd-tag id="sdTag254" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> content was measured and normalized with total protein content. (a) p < 0.001 versus no <sd-tag id="sdTag255" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="GSK3i" ext_ids="176158" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="SB 216763" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">GSK3i</sd-tag> treated pcDNA cells.<graphic href="https://api.sourcedata.io/file.php?panel_id=3078"/></sd-panel><sd-panel panel_id="3079">D-E. Effect of <sd-tag id="sdTag629" source="sdapi" category="entity" entity_type="protein" role="intervention" text="PI3K" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">PI3K</sd-tag> inhibitor <sd-tag id="sdTag262" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> on cellular <sd-tag id="sdTag263" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> content and <sd-tag id="sdTag264" source="sdapi" category="entity" entity_type="molecule" role="component" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> production. Ovarian cancer cells were pretreated with <sd-tag id="sdTag265" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> for at least 2 h in complete media. Cells were then subjected to 2 h<sd-tag id="sdTag268" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag269" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag> withdrawal to deplete endogenous <sd-tag id="sdTag270" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> followed by culturing cells in <sd-tag id="sdTag630" source="sdapi" category="entity" entity_type="undefined" role="component" text="RPMI" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RPMI</sd-tag>-<sd-tag id="sdTag271" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> media in the presence of <sd-tag id="sdTag272" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> for 2 h (2 hSF). After 2 h of <sd-tag id="sdTag631" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="nutrient" ext_ids="CHEBI:33284" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="nutrient" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">nutrient</sd-tag> stress, complete media containing <sd-tag id="sdTag276" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> was added to the cells for 30 min (5% <sd-tag id="sdTag277" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag>) for recovery. A second <sd-tag id="sdTag278" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag279" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag> withdrawal (15 min SF) followed immediately to examine the effect on <sd-tag id="sdTag280" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> and <sd-tag id="sdTag281" source="sdapi" category="entity" entity_type="molecule" role="component" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> levels. a, p < 0.001 5% <sd-tag id="sdTag282" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag> versus 2 hSF, b, p < 0.01 15 min SF versus 5% <sd-tag id="sdTag284" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag>.D. There is an increase in <sd-tag id="sdTag285" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> content during recovery phase (5% <sd-tag id="sdTag286" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag>) and a decrease in <sd-tag id="sdTag287" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> when cells are subject to a second <sd-tag id="sdTag632" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="nutrient" ext_ids="CHEBI:33284" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="nutrient" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">nutrient</sd-tag> stress (15 min SF).<graphic href="https://api.sourcedata.io/file.php?panel_id=3079"/></sd-panel><sd-panel panel_id="3080">D-E. Effect of <sd-tag id="sdTag576" source="sdapi" category="entity" entity_type="gene" role="component" text="PI3K" ext_ids="5290///5291///5294///5293" norm_text="" ext_dbs="NCBI gene///NCBI gene///NCBI gene///NCBI gene" in_caption="True" ext_names="PIK3CA///PIK3CB///PIK3CG///PIK3CD" ext_tax_ids="9606///9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene////http://www.ncbi.nlm.nih.gov/gene/">PI3K</sd-tag> inhibitor <sd-tag id="sdTag294" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> on cellular <sd-tag id="sdTag295" source="sdapi" category="entity" entity_type="molecule" role="component" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> content and <sd-tag id="sdTag296" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> production. Ovarian cancer cells were pretreated with <sd-tag id="sdTag297" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> for at least 2 h in complete media. Cells were then subjected to 2 h<sd-tag id="sdTag300" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag301" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag> withdrawal to deplete endogenous <sd-tag id="sdTag302" source="sdapi" category="entity" entity_type="molecule" role="component" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> followed by culturing cells in <sd-tag id="sdTag578" source="sdapi" category="entity" entity_type="undefined" role="component" text="RPMI" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">RPMI</sd-tag>-<sd-tag id="sdTag303" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> media in the presence of <sd-tag id="sdTag304" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> for 2 h (2 hSF). After 2 h of <sd-tag id="sdTag577" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="nutrient" ext_ids="CHEBI:33284" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="nutrient" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">nutrient</sd-tag> stress, complete media containing <sd-tag id="sdTag308" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> was added to the cells for 30 min (5% <sd-tag id="sdTag309" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag>) for recovery. A second <sd-tag id="sdTag310" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> and <sd-tag id="sdTag311" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag> withdrawal (15 min SF) followed immediately to examine the effect on <sd-tag id="sdTag312" source="sdapi" category="entity" entity_type="molecule" role="component" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> and <sd-tag id="sdTag313" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> levels. a, p < 0.001 5% <sd-tag id="sdTag314" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag> versus 2 hSF, b, p < 0.01 15 min SF versus 5% <sd-tag id="sdTag316" source="sdapi" category="entity" entity_type="undefined" role="intervention" text="FBS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">FBS</sd-tag>.E. Utilization of <sd-tag id="sdTag317" source="sdapi" category="entity" entity_type="molecule" role="component" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> to produce <sd-tag id="sdTag318" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=3080"/></sd-panel><sd-panel panel_id="3081">F. Addition of <sd-tag id="sdTag325" source="sdapi" category="entity" entity_type="protein" role="intervention" text="AKT" ext_ids="Q9Y243///P31749///P31751" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="AKT3///AKT1///AKT2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AKT</sd-tag> inhibitor <sd-tag id="sdTag328" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="MK2206" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">MK2206</sd-tag> (<sd-tag id="sdTag339" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="AKTi" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">AKTi</sd-tag>) abolishes <sd-tag id="sdTag326" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>-dependent <sd-tag id="sdTag327" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> storage. (a) p < 0.05 versus shRNA control and (b) p < 0.01 SF + <sd-tag id="sdTag340" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="AKTi" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">AKTi</sd-tag> versus SF.<graphic href="https://api.sourcedata.io/file.php?panel_id=3081"/></sd-panel><sd-panel panel_id="3082">G. Inhibiting <sd-tag id="sdTag334" source="sdapi" category="entity" entity_type="protein" role="component" text="AKT" ext_ids="Q9Y243///P31749///P31751" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="AKT3///AKT1///AKT2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AKT</sd-tag> pathway and <sd-tag id="sdTag335" source="sdapi" category="entity" entity_type="molecule" role="component" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> metabolism decreases cell viability. Cells were pretreated with 10 µM of <sd-tag id="sdTag336" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> or <sd-tag id="sdTag341" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="AKTi" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">AKTi</sd-tag>, 100 µM <sd-tag id="sdTag342" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="LND" ext_ids="CHEBI:50138" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="lonidamine" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">LND</sd-tag> or <sd-tag id="sdTag343" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="3BrPy" ext_ids="70684" norm_text="" ext_dbs="PubChem" in_caption="True" ext_names="bromopyruvic acid" ext_tax_ids="" ext_tax_names="" ext_urls="https://pubchem.ncbi.nlm.nih.gov/compound/">3BrPy</sd-tag> for 2 h before switching to SF media in the presence of inhibitors for an addition16 h before <sd-tag id="sdTag352" source="sdapi" category="assay" entity_type="" role="" text="cell titre blue viability assay" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">cell titre blue viability assay</sd-tag>. *p < 0.001 shRNA <sd-tag id="sdTag338" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> versus shRNA control.<graphic href="https://api.sourcedata.io/file.php?panel_id=3082"/></sd-panel></fig><fig id="1313"><title></title><label>Figure 4</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1313"/><sd-panel panel_id="3083">A. <sd-tag id="sdTag353" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> over-expression increases <sd-tag id="sdTag581" source="sdapi" category="entity" entity_type="molecule" role="reporter" text="H3" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">H3</sd-tag>-labelled <sd-tag id="sdTag354" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="2DG" ext_ids="CHEBI:15866" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="2-deoxy-D-glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">2DG</sd-tag> uptake. (a) p < 0.001 <sd-tag id="sdTag355" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> versus pcDNA. Results are mean ± s.d. of a triplicate in one of representative experiment.<graphic href="https://api.sourcedata.io/file.php?panel_id=3083"/></sd-panel><sd-panel panel_id="3084">B. Down-regulation of <sd-tag id="sdTag361" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> or <sd-tag id="sdTag362" source="sdapi" category="entity" entity_type="gene" role="intervention" text="AKT" ext_ids="207" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="AKT1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">AKT</sd-tag> expression, or inhibition <sd-tag id="sdTag582" source="sdapi" category="entity" entity_type="gene" role="intervention" text="AKT" ext_ids="207" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="AKT1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">AKT</sd-tag> activity by <sd-tag id="sdTag364" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="MK2206" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">MK2206</sd-tag> decreases <sd-tag id="sdTag365" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> uptake in <sd-tag id="sdTag366" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HEY" ext_ids="CVCL_0297" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HEY" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HEY</sd-tag> cells (a) p < 0.001 versus NT siRNA control, (b) p < 0.05 versus siRab25 and (c) p < 0.05 versus siAKT.<graphic href="https://api.sourcedata.io/file.php?panel_id=3084"/></sd-panel><sd-panel panel_id="3085">C. Effect of down regulation of <sd-tag id="sdTag368" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> and <sd-tag id="sdTag584" source="sdapi" category="entity" entity_type="gene" role="component" text="AKT" ext_ids="207" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="AKT1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">AKT</sd-tag> inhibitor <sd-tag id="sdTag644" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="MK2206" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">MK2206</sd-tag> (<sd-tag id="sdTag646" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="AKTi" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">AKTi</sd-tag>) on <sd-tag id="sdTag372" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> uptake. Cells were either pretreated for 2 h with <sd-tag id="sdTag645" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="AKTi" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">AKTi</sd-tag> before assessing <sd-tag id="sdTag375" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glucose" ext_ids="CHEBI:17234" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glucose</sd-tag> uptake. (a) p < 0.05 versus shRNA control and (b) p < 0.05 versus shRab25.<graphic href="https://api.sourcedata.io/file.php?panel_id=3085"/></sd-panel><sd-panel panel_id="3086">D. <sd-tag id="sdTag379" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Rab25" ext_ids="P57735" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Rab25</sd-tag> co-localization with <sd-tag id="sdTag383" source="sdapi" category="entity" entity_type="protein" role="assayed" text="GLUT1" ext_ids="P11166" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="SLC2A1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">GLUT1</sd-tag> in <sd-tag id="sdTag380" source="sdapi" category="entity" entity_type="cell_line" role="component" text="A2780" ext_ids="CVCL_0134" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="A2780" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">A2780</sd-tag> cells. <sd-tag id="sdTag639" source="sdapi" category="assay" entity_type="" role="" text="Immunofluorescence microscopy" ext_ids="BAO_0000450" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="fluorescence microscopy" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">Immunofluorescence microscopy</sd-tag> staining of <sd-tag id="sdTag384" source="sdapi" category="entity" entity_type="protein" role="assayed" text="GLUT1" ext_ids="P11166" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="SLC2A1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">GLUT1</sd-tag> (red) and <sd-tag id="sdTag381" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Rab25" ext_ids="P57735" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Rab25</sd-tag> (green). Co-localization of <sd-tag id="sdTag385" source="sdapi" category="entity" entity_type="protein" role="assayed" text="GLUT1" ext_ids="P11166" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="SLC2A1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">GLUT1</sd-tag> and <sd-tag id="sdTag382" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Rab25" ext_ids="P57735" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Rab25</sd-tag> (i.e. yellow) is indicated by arrows. Single scale bar, 10 µM.<graphic href="https://api.sourcedata.io/file.php?panel_id=3086"/></sd-panel><sd-panel panel_id="3087">E-G. Resistance to <sd-tag id="sdTag386" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="2DG" ext_ids="CHEBI:15866" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="2-deoxy-D-glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">2DG</sd-tag> induced cell death in <sd-tag id="sdTag387" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> cells is mediated through the PI3K/AKT pathway. Inhibition of the PI3K/AKT pathway was achieved by addition of 10 µM of <sd-tag id="sdTag509" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> or <sd-tag id="sdTag510" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="MK2206" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">MK2206</sd-tag>.E. AKT inhibition increases sensitivity to <sd-tag id="sdTag393" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="2DG" ext_ids="CHEBI:15866" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="2-deoxy-D-glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">2DG</sd-tag> induced cell death. *p < 0.001 versus <sd-tag id="sdTag394" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="HEY" ext_ids="CVCL_0297" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HEY" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HEY</sd-tag>pcDNA cells.<graphic href="https://api.sourcedata.io/file.php?panel_id=3087"/></sd-panel><sd-panel panel_id="3088">E-G. Resistance to <sd-tag id="sdTag399" source="sdapi" category="entity" entity_type="molecule" role="component" text="2DG" ext_ids="CHEBI:15866" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="2-deoxy-D-glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">2DG</sd-tag> induced cell death in <sd-tag id="sdTag400" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> cells is mediated through the PI3K/AKT pathway. Inhibition of the PI3K/AKT pathway was achieved by addition of 10 µM of <sd-tag id="sdTag403" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> or <sd-tag id="sdTag404" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="MK2206" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">MK2206</sd-tag>.F. Down-regulation of <sd-tag id="sdTag408" source="sdapi" category="entity" entity_type="gene" role="intervention" text="AKT" ext_ids="207" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="AKT1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">AKT</sd-tag> by siRNA specific to AKT.<graphic href="https://api.sourcedata.io/file.php?panel_id=3088"/></sd-panel><sd-panel panel_id="3089">E-G. Resistance to <sd-tag id="sdTag410" source="sdapi" category="entity" entity_type="molecule" role="component" text="2DG" ext_ids="CHEBI:15866" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="2-deoxy-D-glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">2DG</sd-tag> induced cell death in <sd-tag id="sdTag411" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> cells is mediated through the PI3K/AKT pathway. Inhibition of the PI3K/AKT pathway was achieved by addition of 10 µM of <sd-tag id="sdTag414" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="PI103" ext_ids="CHEBI:90524" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="PI-103" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">PI103</sd-tag> or <sd-tag id="sdTag415" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="MK2206" ext_ids="CHEBI:67271" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="MK-2206" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">MK2206</sd-tag>.G. Or down-regulation of <sd-tag id="sdTag416" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> by shRNA specific to <sd-tag id="sdTag417" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> enhances <sd-tag id="sdTag418" source="sdapi" category="entity" entity_type="molecule" role="component" text="2DG" ext_ids="CHEBI:15866" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="2-deoxy-D-glucose" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">2DG</sd-tag> induced cell death. *p < 0.001 versus RNAi control cells.<graphic href="https://api.sourcedata.io/file.php?panel_id=3089"/></sd-panel></fig><fig id="1314"><title></title><label>Figure 5</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1314"/><sd-panel panel_id="3090">Ovarian cancer <sd-tag id="sdTag424" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="HEY" ext_ids="CVCL_0297" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HEY" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HEY</sd-tag> cells (upper panel) were co-transfected with <sd-tag id="sdTag425" source="sdapi" category="entity" entity_type="gene" role="component" text="AKT" ext_ids="207" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="AKT1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">AKT</sd-tag>-<sd-tag id="sdTag433" source="sdapi" category="entity" entity_type="protein" role="reporter" text="IFPN" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">IFPN</sd-tag> and <sd-tag id="sdTag426" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>-<sd-tag id="sdTag434" source="sdapi" category="entity" entity_type="protein" role="reporter" text="IFPC" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">IFPC</sd-tag> or with <sd-tag id="sdTag427" source="sdapi" category="entity" entity_type="gene" role="component" text="AKT" ext_ids="207" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="AKT1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">AKT</sd-tag>-<sd-tag id="sdTag511" source="sdapi" category="entity" entity_type="protein" role="reporter" text="IFPC" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">IFPC</sd-tag> and <sd-tag id="sdTag428" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>-<sd-tag id="sdTag512" source="sdapi" category="entity" entity_type="protein" role="reporter" text="IFPN" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">IFPN</sd-tag>. Cells become fluorescent due to an interaction between <sd-tag id="sdTag429" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AKT" ext_ids="Q9Y243///P31749///P31751" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="AKT3///AKT1///AKT2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AKT</sd-tag> and <sd-tag id="sdTag430" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Rab25" ext_ids="P57735" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Rab25</sd-tag> bringing the two halves of the fluorescent protein into proximity creating a stable complex and restoring fluorescence. <sd-tag id="sdTag436" source="sdapi" category="entity" entity_type="cell_line" role="experiment" text="Hela" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">Hela</sd-tag> cells stably expressing <sd-tag id="sdTag431" source="sdapi" category="entity" entity_type="gene" role="component" text="AKT" ext_ids="207" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="AKT1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">AKT</sd-tag>-<sd-tag id="sdTag513" source="sdapi" category="entity" entity_type="protein" role="reporter" text="IFPN" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">IFPN</sd-tag> (lower panel) were transfected with <sd-tag id="sdTag432" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>-<sd-tag id="sdTag514" source="sdapi" category="entity" entity_type="protein" role="reporter" text="IFPC" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">IFPC</sd-tag> or <sd-tag id="sdTag435" source="sdapi" category="entity" entity_type="gene" role="component" text="PDK" ext_ids="5170" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="PDPK1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">PDK</sd-tag>-<sd-tag id="sdTag515" source="sdapi" category="entity" entity_type="protein" role="reporter" text="IFPC" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">IFPC</sd-tag> as control. A brightfield image corresponding to the fluorescent image is shown and transfected cells indicated by an arrow.<graphic href="https://api.sourcedata.io/file.php?panel_id=3090"/></sd-panel><sd-panel panel_id="3091">Detection of <sd-tag id="sdTag438" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> and <sd-tag id="sdTag439" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AKT" ext_ids="Q9Y243///P31749///P31751" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="AKT3///AKT1///AKT2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AKT</sd-tag> interaction by <sd-tag id="sdTag442" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. Ovarian cells expressing <sd-tag id="sdTag443" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-tagged <sd-tag id="sdTag440" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Rab25" ext_ids="P57735" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Rab25</sd-tag> were IP with anti-<sd-tag id="sdTag444" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag> antibody. The resultant complex was separated by <sd-tag id="sdTag590" source="sdapi" category="assay" entity_type="" role="" text="gel electrophoresis" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">gel electrophoresis</sd-tag> and detected by <sd-tag id="sdTag633" source="sdapi" category="assay" entity_type="" role="" text="WB" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">WB</sd-tag> using anti-<sd-tag id="sdTag441" source="sdapi" category="entity" entity_type="protein" role="assayed" text="AKT" ext_ids="Q9Y243///P31749///P31751" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="AKT3///AKT1///AKT2" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">AKT</sd-tag> antibody.<graphic href="https://api.sourcedata.io/file.php?panel_id=3091"/></sd-panel><sd-panel panel_id="3092">Generation of <sd-tag id="sdTag450" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> deletion mutants. Expression of the deletion mutants was detected by <sd-tag id="sdTag593" source="sdapi" category="assay" entity_type="" role="" text="immunofluorescence staining" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">immunofluorescence staining</sd-tag> (upper panel) and <sd-tag id="sdTag634" source="sdapi" category="assay" entity_type="" role="" text="WB" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">WB</sd-tag> (middle panel) using anti-<sd-tag id="sdTag453" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> antibody which interacts with <sd-tag id="sdTag454" source="sdapi" category="entity" entity_type="protein" role="reporter" text="YFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">YFP</sd-tag>. Diagrammatic representation of the structure of the mutants is shown in the lower panel.<graphic href="https://api.sourcedata.io/file.php?panel_id=3092"/></sd-panel><sd-panel panel_id="3093"><sd-tag id="sdTag455" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> deletion mutants do not alter cellular <sd-tag id="sdTag456" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> and <sd-tag id="sdTag457" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> levels. Ovarian cancer cell lysates were collected 24 h post transfection. (a) p < 0.05 versus empty vector transfected cells.<graphic href="https://api.sourcedata.io/file.php?panel_id=3093"/></sd-panel></fig><fig id="1315"><title></title><label>Figure 6</label><graphic href="https://api.sourcedata.io/file.php?figure_id=1315"/><sd-panel panel_id="3094">A. <sd-tag id="sdTag464" source="sdapi" category="entity" entity_type="gene" role="assayed" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression correlates with <sd-tag id="sdTag465" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> levels in <sd-tag id="sdTag595" source="sdapi" category="entity" entity_type="organism" role="experiment" text="patient" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patient</sd-tag><sd-tag id="sdTag596" source="sdapi" category="disease" entity_type="" role="" text="tumours" ext_ids="C04" norm_text="" ext_dbs="MeSH" in_caption="True" ext_names="Neoplasms" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ncbi.nlm.nih.gov/mesh/?term=">tumours</sd-tag>. Total RNA and total cellular extracts, isolated from 31 ovarian cancer <sd-tag id="sdTag597" source="sdapi" category="entity" entity_type="organism" role="experiment" text="patient" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patient</sd-tag> specimens, were subjected to <sd-tag id="sdTag466" source="sdapi" category="entity" entity_type="gene" role="assayed" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> gene expression and <sd-tag id="sdTag467" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="glycogen" ext_ids="CHEBI:28087" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="glycogen" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">glycogen</sd-tag> content analysis using <sd-tag id="sdTag635" source="sdapi" category="assay" entity_type="" role="" text="qPCR" ext_ids="BAO_0002084" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="real-time PCR " ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">qPCR</sd-tag> and glycogen assay, respectively.<graphic href="https://api.sourcedata.io/file.php?panel_id=3094"/></sd-panel><sd-panel panel_id="3095">B-C. The <sd-tag id="sdTag472" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> expression signature identifies <sd-tag id="sdTag599" source="sdapi" category="entity" entity_type="organism" role="assayed" text="patients" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patients</sd-tag> with a poor prognosis. Ovarian cancer <sd-tag id="sdTag600" source="sdapi" category="entity" entity_type="organism" role="assayed" text="patients" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patients</sd-tag> were classified as either mirroring the <sd-tag id="sdTag473" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>-associated gene expression signature (<sd-tag id="sdTag474" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> signature) or not (non-<sd-tag id="sdTag475" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> signature) using linear discriminant analysis in BRB tools, in two independent published ovarian datasets. Progression free <sd-tag id="sdTag606" source="sdapi" category="assay" entity_type="" role="" text="survival curves" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls=""><sd-pretag id="sdPretag14383268010060" parent-tag-id="610">survival</sd-pretag> curves</sd-tag>for <sd-tag id="sdTag601" source="sdapi" category="entity" entity_type="organism" role="assayed" text="patients" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patients</sd-tag> with advanced disease (Stage II to IV) are shown. Univariate analyses were plotted using Kaplan-Meier method and Coxplots used for multivariate analyses (co-variates included stage, grade, histology and residual disease).B. Tothill et al, 2008.C. Dressman et al, 2007.<graphic href="https://api.sourcedata.io/file.php?panel_id=3095"/></sd-panel><sd-panel panel_id="3096">D. Prediction of breast cancer overall <sd-tag id="sdTag610" source="sdapi" category="assay" entity_type="" role="" text="survival" ext_ids="OBI_0000699" norm_text="" ext_dbs="OBI" in_caption="True" ext_names="survival assessment" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/">survival</sd-tag> in two independent published breast datasets (upper panel) Pawitan et al, 2005 and (lower panel) Chin et al, 2006, based on <sd-tag id="sdTag477" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>-associated gene signature. Breast <sd-tag id="sdTag607" source="sdapi" category="entity" entity_type="organism" role="assayed" text="patient" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patient</sd-tag> classification was determined by BRB tool class prediction function to identify <sd-tag id="sdTag608" source="sdapi" category="entity" entity_type="organism" role="assayed" text="patient" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patient</sd-tag> with <sd-tag id="sdTag478" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>-associated gene expression signature (<sd-tag id="sdTag479" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag>-signature) or without (non-<sd-tag id="sdTag480" source="sdapi" category="entity" entity_type="gene" role="component" text="Rab25" ext_ids="57111" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="RAB25" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Rab25</sd-tag> signature). All <sd-tag id="sdTag609" source="sdapi" category="entity" entity_type="organism" role="assayed" text="patient" ext_ids="9606" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Homo sapiens" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">patients</sd-tag> from the datasets were included in class prediction.<graphic href="https://api.sourcedata.io/file.php?panel_id=3096"/></sd-panel></fig></article>