initial commit

construct.py

@@ -0,0 +1,49 @@
+######## GRADIO INPUT ########
+
+import gradio as gr
+from ConstructBuilder.builder import build
+from Bio import SeqIO
+import pandas as pd
+import uuid
+import os
+
+def LabFusion(name, file , uni_seq , orientation:str="NC", gfp:bool=False, sol_tag:list=[]):
+    dick = {}
+    if file:
+        sequences = SeqIO.to_dict(SeqIO.parse(file.name, "fasta"))
+        
+        for key in sequences:
+            seq = sequences[key]
+            dick[key] = build(str(seq.seq), sol_tag, gfp, orientation) 
+    else:
+        dick[name] = build(uni_seq, sol_tag, gfp, orientation) 
+    table = pd.DataFrame.from_dict(dick)
+    
+    table.to_csv(f"/home/ubuntu/1TB_disk/constructos/gradio_projects/constructs_{name}.csv")
+    return table, f"/home/ubuntu/1TB_disk/constructos/gradio_projects/constructs_{name}.csv"
+
+
+int_align = gr.Interface(fn=LabFusion,
+                         inputs=[ 
+                            gr.Textbox(label="Project name"),   
+                            gr.File(label="Insert a Fasta file"),
+                            gr.Textbox(label="Insert a unique amino acid sequence"),
+                            gr.Dropdown(choices=["N","C", "N+C"], label="Protein Orientarion"),
+                            gr.Checkbox(value=False, label="do you want GFP in your constructs?"),
+                            gr.CheckboxGroup(choices=['MBP', 'SUMO', 'Fh8', 'GST',
+                                                       'NusA', 'Thioredoxin', 'FLAG', 
+                                                       'S-tag', 'CBP', 'STREPII', 'BAP', 'NT11',
+                                                       'Tab2', 'Z-basic', 'ProteinA', 'IMPACT', 
+                                                       'mysB', 'PolyR', 'c-myc', 'S', 'SBP-tag',
+                                                       'Strep-tag', 'Twin-Strep-tag', 'HAT', 
+                                                       'BCCP', 'HaloTag','all'], label="Solubility Tags")],
+                            outputs=[gr.DataFrame(label = " Constructs ") ,
+                                     gr.File(label="CSV output file")],
+                            title="ConstructMaker",
+                            live=True
+                        )
+
+inter = gr.TabbedInterface([int_align],
+                           ["LabFusion"])
+
+inter.launch(share=True)