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| ######## GRADIO INPUT ######## | |
| import gradio as gr | |
| from ConstructBuilder.builder import build | |
| from Bio import SeqIO | |
| import pandas as pd | |
| import uuid | |
| import os | |
| def LabFusion(name, file , uni_seq , orientation:str="NC", gfp:bool=False, sol_tag:list=[]): | |
| dick = {} | |
| if file: | |
| sequences = SeqIO.to_dict(SeqIO.parse(file.name, "fasta")) | |
| for key in sequences: | |
| seq = sequences[key] | |
| dick[key] = build(str(seq.seq), sol_tag, gfp, orientation) | |
| else: | |
| dick[name] = build(uni_seq, sol_tag, gfp, orientation) | |
| table = pd.DataFrame.from_dict(dick) | |
| table.to_csv(f"/home/ubuntu/1TB_disk/constructos/gradio_projects/constructs_{name}.csv") | |
| return table, f"/home/ubuntu/1TB_disk/constructos/gradio_projects/constructs_{name}.csv" | |
| int_align = gr.Interface(fn=LabFusion, | |
| inputs=[ | |
| gr.Textbox(label="Project name"), | |
| gr.File(label="Insert a Fasta file"), | |
| gr.Textbox(label="Insert a unique amino acid sequence"), | |
| gr.Dropdown(choices=["N","C", "N+C"], label="Protein Orientarion"), | |
| gr.Checkbox(value=False, label="do you want GFP in your constructs?"), | |
| gr.CheckboxGroup(choices=['MBP', 'SUMO', 'Fh8', 'GST', | |
| 'NusA', 'Thioredoxin', 'FLAG', | |
| 'S-tag', 'CBP', 'STREPII', 'BAP', 'NT11', | |
| 'Tab2', 'Z-basic', 'ProteinA', 'IMPACT', | |
| 'mysB', 'PolyR', 'c-myc', 'S', 'SBP-tag', | |
| 'Strep-tag', 'Twin-Strep-tag', 'HAT', | |
| 'BCCP', 'HaloTag','all'], label="Solubility Tags")], | |
| outputs=[gr.DataFrame(label = " Constructs ") , | |
| gr.File(label="CSV output file")], | |
| title="ConstructMaker", | |
| live=True | |
| ) | |
| inter = gr.TabbedInterface([int_align], | |
| ["LabFusion"]) | |
| inter.launch(share=True) |