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CRISPRTool

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LfOreVEr commited on Apr 7

Commit

6664381

•

1 Parent(s): c2ffe9b

Update cas9on.py

Files changed (1) hide show

cas9on.py CHANGED Viewed

@@ -83,7 +83,7 @@ def find_crispr_targets(sequence, chr, start, strand, transcript_id, exon_id, pa
             if i >= target_length:
                 target_seq = sequence[i - target_length:i + 3]
                 tar_start = start + i - target_length
-                tar_end = start + i + 3
                 gRNA = ''.join([dnatorna[base] for base in sequence[i - target_length:i]])
                 targets.append([target_seq, gRNA, chr, str(tar_start), str(tar_end), str(strand), transcript_id, exon_id])

             if i >= target_length:
                 target_seq = sequence[i - target_length:i + 3]
                 tar_start = start + i - target_length
+                tar_end = start + i + 3 - 1
                 gRNA = ''.join([dnatorna[base] for base in sequence[i - target_length:i]])
                 targets.append([target_seq, gRNA, chr, str(tar_start), str(tar_end), str(strand), transcript_id, exon_id])