domain2go_app.py

import streamlit as st
import requests
from io import StringIO
from Bio import SeqIO
import os
import time
import pandas as pd

from run_domain2go_app import *


def convert_df(df):
   return df.to_csv(index=False).encode('utf-8')


st.markdown("""
<div style="background-color:#f9f9f9;padding:10px">
<p style="color:#b22d2a;font-size:15px;">Disclaimer</p>
<p style="color:#000000;font-size:14px;">This program is designed to generate predictions for a single protein due to the extended runtime of InterProScan. If you need predictions for multiple UniProtKB/Swiss-Prot proteins, we recommend utilizing our comprehensive protein function prediction dataset available in our <a href="https://github.com/HUBioDataLab/Domain2GO">Github repository</a>.</p>
</div>
""", unsafe_allow_html=True)



domain_tab, pred_tab = st.tabs(['Domains', 'Function predictions'])

with domain_tab:
    st.header('Domains in sequence')

with st.sidebar:

    st.title("Domain2GO: Mutual Annotation-Based Prediction of Protein Domain Functions")
    st.write("[![arXiv](https://img.shields.io/badge/bioRxiv-2022.11.03.514980-b31b1b.svg)](https://www.biorxiv.org/content/10.1101/2022.11.03.514980v1) [![github-repository](https://img.shields.io/badge/GitHub-black?logo=github)](https://github.com/HUBioDataLab/Domain2GO)")

    if 'example_seq_button' not in st.session_state:
        st.session_state.example_seq_button = False

    def click_button():
        st.session_state.example_seq_button = not st.session_state.example_seq_button
    
    input_type = st.radio('Select input type', ['Enter sequence', 'Upload FASTA file'])
    if input_type == 'Enter sequence':
        if st.session_state.example_seq_button:
            sequence = st.text_area('Enter protein sequence in FASTA format.', 
                value='>sp|O18783|PLMN_NOTEU\n'
                'MEYGKVIFLFLLFLKSGQGESLENYIKTEGASLSNSQKKQFVASSTEECEALCEKETEFVCRSFEHYNKEQKCVIMSENSKTSSVERKRDVVLFEKRIYLSDCKSGNGRNYRGTLSKTKSGITCQKWSDLSPHVPNYAPSKYPDAGLEKNYCRNPDDDVKGPWCYTTNPDIRYEYCDVPECEDECMHCSGENYRGTISKTESGIECQPWDSQEPHSHEYIPSKFPSKDLKENYCRNPDGEPRPWCFTSNPEKRWEFCNIPRCSSPPPPPGPMLQCLKGRGENYRGKIAVTKSGHTCQRWNKQTPHKHNRTPENFPCRGLDENYCRNPDGELEPWCYTTNPDVRQEYCAIPSCGTSSPHTDRVEQSPVIQECYEGKGENYRGTTSTTISGKKCQAWSSMTPHQHKKTPDNFPNADLIRNYCRNPDGDKSPWCYTMDPTVRWEFCNLEKCSGTGSTVLNAQTTRVPSVDTTSHPESDCMYGSGKDYRGKRSTTVTGTLCQAWTAQEPHRHTIFTPDTYPRAGLEENYCRNPDGDPNGPWCYTTNPKKLFDYCDIPQCVSPSSFDCGKPRVEPQKCPGRIVGGCYAQPHSWPWQISLRTRFGEHFCGGTLIAPQWVLTAAHCLERSQWPGAYKVILGLHREVNPESYSQEIGVSRLFKGPLAADIALLKLNRPAAINDKVIPACLPSQDFMVPDRTLCHVTGWGDTQGTSPRGLLKQASLPVIDNRVCNRHEYLNGRVKSTELCAGHLVGRGDSCQGDSGGPLICFEDDKYVLQGVTSWGLGCARPNKPGVYVRVSRYISWIEDVMKNN')
        else:
            sequence = st.text_input('Enter protein sequence in FASTA format.')
        name = sequence.split('\n')[0].strip('>')
        st.button('Use example sequence', on_click=click_button)
    else:
        protein_input = st.file_uploader('Choose file')
        if protein_input:
            protein_input_stringio = StringIO(protein_input.getvalue().decode("utf-8"))
            fasta_sequences = SeqIO.parse(protein_input_stringio, 'fasta')
            for fasta in fasta_sequences:
                name, sequence = fasta.id, str(fasta.seq)

    email = st.text_input('Enter your email for InterProScan query: ')

    # prevent user from clicking 'Find domains' button if email or sequence is empty
    domains_submitted = False  
    if st.button('Find domains'):
        if email and sequence:
            domains_submitted = True
            st.session_state.disabled = True
        else:
            st.warning('Please enter your email and protein sequence first.')
    else:
        with domain_tab:
            st.warning('Please enter your query and click "Find domains" to see domains in sequence.')
with domain_tab:
    no_domains = False
    error_in_interproscan = False
    if domains_submitted:
        with st.spinner('Finding domains in sequence using InterProScan. This may take a while...'):
            result = find_domains(email, sequence, name)
        result_text = result[0]
        if result_text == 'Domains found.':
            st.success(result_text + 'You can now see function predictions for the sequence in the "Function predictions" tab.')
            st.session_state['domain_df'] = result[1]
        elif result_text == 'No domains found.':
            st.warning(result_text)
            no_domains = True
        else:
            st.error(result_text)
            st.write(f'InterProScan job id: {result[1]}')
            st.write(f'InterProScan job response: {result[2]}')
            error_in_interproscan = True


    if 'domain_df' in st.session_state:
        with st.expander('Show domains in sequence'):
            st.write(st.session_state.domain_df)
            domains_csv = convert_df(st.session_state.domain_df)
            st.download_button(
                label="Download domains in sequence as CSV",
                data=domains_csv,
                file_name=f"{name}_domains.csv",
                mime="text/csv",
            )

with pred_tab:
    st.header('Function predictions')
    if 'domain_df' not in st.session_state:
        if no_domains:
            st.warning('No domains found. Please find domains in sequence first.')
        elif error_in_interproscan:
            st.error('Error in InterProScan. Please check InterProScan job id and response.')
        else:
            st.warning('Please find domains in sequence first.')
    else:
        with st.spinner('Generating function predictions...'):
            cwd = os.getcwd()
            mapping_path = "{}Domain2GO/data".format(cwd.split("Domain2GO")[0])
            pred_results = generate_function_predictions(st.session_state.domain_df, mapping_path)
            pred_result_text = pred_results[0]
            if pred_result_text == 'Function predictions found.':
                st.success(pred_result_text)
                st.session_state['pred_df'] = pred_results[1]
            elif pred_result_text == 'No function predictions found.':
                st.warning(pred_result_text)

        if 'pred_df' in st.session_state:
            with st.expander('Show function predictions'):
                st.write(st.session_state.pred_df)
                pred_csv = convert_df(st.session_state.pred_df)
                st.download_button(
                    label="Download function predictions as CSV",
                    data=pred_csv,
                    file_name=f"{name}_function_predictions.csv",
                    mime="text/csv",
                )