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  1. .config/.last_opt_in_prompt.yaml +1 -0
  2. .config/.last_survey_prompt.yaml +1 -0
  3. .config/.last_update_check.json +1 -0
  4. .config/access_tokens.db +0 -0
  5. .config/active_config +1 -0
  6. .config/application_default_credentials.json +9 -0
  7. .config/config_sentinel +0 -0
  8. .config/configurations/config_default +9 -0
  9. .config/credentials.db +0 -0
  10. .config/default_configs.db +0 -0
  11. .config/gce +1 -0
  12. .config/hidden_gcloud_config_universe_descriptor_data_cache_configs.db +0 -0
  13. .config/legacy_credentials/yiqu.rising@gmail.com/.boto +6 -0
  14. .config/legacy_credentials/yiqu.rising@gmail.com/adc.json +8 -0
  15. .config/logs/2025.11.05/14.33.13.470069.log +765 -0
  16. .config/logs/2025.11.05/14.33.36.385956.log +5 -0
  17. .config/logs/2025.11.05/14.33.44.287731.log +153 -0
  18. .config/logs/2025.11.05/14.33.45.559498.log +5 -0
  19. .config/logs/2025.11.05/14.33.53.434728.log +8 -0
  20. .config/logs/2025.11.05/14.33.54.129583.log +8 -0
  21. .config/logs/2025.11.08/03.31.43.532264.log +8 -0
  22. .config/logs/2025.11.08/03.38.30.158621.log +18 -0
  23. .config/logs/2025.11.08/03.38.33.972101.log +9 -0
  24. .config/logs/2025.11.08/03.41.52.318962.log +30 -0
  25. .config/logs/2025.11.08/03.41.54.648119.log +39 -0
  26. .config/logs/2025.11.08/03.46.31.985987.log +8 -0
  27. .config/logs/2025.11.08/03.46.32.585013.log +8 -0
  28. .config/logs/2025.11.08/03.56.10.930875.log +15 -0
  29. .config/logs/2025.11.08/03.56.28.454394.log +22 -0
  30. .config/logs/2025.11.08/03.56.56.000749.log +8 -0
  31. .config/logs/2025.11.08/03.56.56.815151.log +8 -0
  32. .config/logs/2025.11.08/04.00.48.739317.log +8 -0
  33. .config/logs/2025.11.08/04.00.49.407053.log +8 -0
  34. .config/logs/2025.11.08/04.02.17.927382.log +8 -0
  35. .config/logs/2025.11.08/04.02.19.083433.log +8 -0
  36. .config/logs/2025.11.08/04.04.13.667264.log +8 -0
  37. .config/logs/2025.11.08/04.04.14.707530.log +8 -0
  38. .gitattributes +9 -0
  39. .gradio/certificate.pem +31 -0
  40. README.md +2 -8
  41. app.py +67 -0
  42. data/hplc.jsonl +100 -0
  43. hplc_lora/README.md +210 -0
  44. hplc_lora/adapter_config.json +46 -0
  45. hplc_lora/adapter_model.safetensors +3 -0
  46. hplc_lora/chat_template.jinja +5 -0
  47. hplc_lora/special_tokens_map.json +17 -0
  48. hplc_lora/tokenizer.json +3 -0
  49. hplc_lora/tokenizer_config.json +2064 -0
  50. huggingface_tokenizers_cache/.locks/models--unsloth--llama-3-8b-Instruct-bnb-4bit/348af68926a93e44c8f013e5d4d180b37a569570.lock +0 -0
.config/.last_opt_in_prompt.yaml ADDED
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.config/.last_update_check.json ADDED
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+ {"last_update_check_time": 1762353225.0701716, "last_update_check_revision": 20251031202127, "notifications": [], "last_nag_times": {}}
.config/access_tokens.db ADDED
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.config/active_config ADDED
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.config/configurations/config_default ADDED
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+ [component_manager]
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+ disable_update_check = true
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+ [compute]
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+ [core]
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+ project = coherent-ascent-464321-s9
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.config/legacy_credentials/yiqu.rising@gmail.com/.boto ADDED
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.config/logs/2025.11.05/14.33.13.470069.log ADDED
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+ 2025-11-05 14:33:25,498 DEBUG root Loaded Command Group: ['gcloud', 'components']
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+ 2025-11-05 14:33:25,501 DEBUG root Loaded Command Group: ['gcloud', 'components', 'update']
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+ 2025-11-05 14:33:25,504 INFO ___FILE_ONLY___ Beginning update. This process may take several minutes.
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+
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+ 2025-11-05 14:33:25,569 DEBUG root Chosen display Format:table[box,title="These components will be updated."](details.display_name:label=Name:align=left,version.version_string:label=Version:align=right,data.size.size(zero="",min=1048576):label=Size:align=right)
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+ 2025-11-05 14:33:25,569 DEBUG root Chosen display Format:table[box,title="These components will be installed."](details.display_name:label=Name:align=left,version.version_string:label=Version:align=right,data.size.size(zero="",min=1048576):label=Size:align=right)
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+ 2025-11-05 14:33:25,611 INFO ___FILE_ONLY___ ┌──────────────────────────────────────────────────────────────────────────────┐
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+ 2025-11-05 14:33:27,117 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/components/google-cloud-sdk-gsutil-nix-20251031202127.tar.gz HTTP/1.1" 200 1949
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+ 2025-11-05 14:33:27,151 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/components/google-cloud-sdk-core-nix-20251031202127.tar.gz HTTP/1.1" 200 2326
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+ 2025-11-05 14:33:27,171 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/components/google-cloud-sdk-gcloud-crc32c-linux-x86_64-20251031202127.tar.gz HTTP/1.1" 200 1531280
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+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
458
+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
461
+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
462
+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,185 INFO ___FILE_ONLY___ ═
466
+ 2025-11-05 14:33:27,186 INFO ___FILE_ONLY___ ═
467
+ 2025-11-05 14:33:27,186 INFO ___FILE_ONLY___ ═
468
+ 2025-11-05 14:33:27,186 INFO ___FILE_ONLY___ ═
469
+ 2025-11-05 14:33:27,186 INFO ___FILE_ONLY___ ═
470
+ 2025-11-05 14:33:27,186 INFO ___FILE_ONLY___ ═
471
+ 2025-11-05 14:33:27,186 INFO ___FILE_ONLY___ ═
472
+ 2025-11-05 14:33:27,186 INFO ___FILE_ONLY___ ═
473
+ 2025-11-05 14:33:27,186 INFO ___FILE_ONLY___ ╝
474
+
475
+ 2025-11-05 14:33:27,188 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
476
+
477
+ 2025-11-05 14:33:27,188 INFO ___FILE_ONLY___ ╠═ Downloading: gcloud cli dependencies (Platform Specific) ═╣
478
+
479
+ 2025-11-05 14:33:27,188 INFO ___FILE_ONLY___ ╚
480
+ 2025-11-05 14:33:27,190 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): dl.google.com:443
481
+ 2025-11-05 14:33:27,201 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/components/google-cloud-sdk-gcloud-deps-linux-x86_64-20210416153011.tar.gz HTTP/1.1" 200 104
482
+ 2025-11-05 14:33:27,201 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
483
+ 2025-11-05 14:33:27,201 INFO ___FILE_ONLY___ ╝
484
+
485
+ 2025-11-05 14:33:27,203 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
486
+
487
+ 2025-11-05 14:33:27,203 INFO ___FILE_ONLY___ ╠═ Installing: BigQuery Command Line Tool ═╣
488
+
489
+ 2025-11-05 14:33:27,203 INFO ___FILE_ONLY___ ╚
490
+ 2025-11-05 14:33:27,302 INFO ___FILE_ONLY___ ═
491
+ 2025-11-05 14:33:27,304 INFO ___FILE_ONLY___ ═
492
+ 2025-11-05 14:33:27,307 INFO ___FILE_ONLY___ ═
493
+ 2025-11-05 14:33:27,309 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,312 INFO ___FILE_ONLY___ ═
495
+ 2025-11-05 14:33:27,315 INFO ___FILE_ONLY___ ═
496
+ 2025-11-05 14:33:27,317 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,319 INFO ___FILE_ONLY___ ═
498
+ 2025-11-05 14:33:27,321 INFO ___FILE_ONLY___ ═
499
+ 2025-11-05 14:33:27,324 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,326 INFO ___FILE_ONLY___ ═
501
+ 2025-11-05 14:33:27,328 INFO ___FILE_ONLY___ ═
502
+ 2025-11-05 14:33:27,330 INFO ___FILE_ONLY___ ═
503
+ 2025-11-05 14:33:27,332 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,335 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,337 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,339 INFO ___FILE_ONLY___ ═
507
+ 2025-11-05 14:33:27,342 INFO ___FILE_ONLY___ ═
508
+ 2025-11-05 14:33:27,345 INFO ___FILE_ONLY___ ═
509
+ 2025-11-05 14:33:27,347 INFO ___FILE_ONLY___ ═
510
+ 2025-11-05 14:33:27,349 INFO ___FILE_ONLY___ ═
511
+ 2025-11-05 14:33:27,351 INFO ___FILE_ONLY___ ═
512
+ 2025-11-05 14:33:27,353 INFO ___FILE_ONLY___ ═
513
+ 2025-11-05 14:33:27,355 INFO ___FILE_ONLY___ ═
514
+ 2025-11-05 14:33:27,358 INFO ___FILE_ONLY___ ═
515
+ 2025-11-05 14:33:27,360 INFO ___FILE_ONLY___ ═
516
+ 2025-11-05 14:33:27,361 INFO ___FILE_ONLY___ ═
517
+ 2025-11-05 14:33:27,363 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:27,365 INFO ___FILE_ONLY___ ═
519
+ 2025-11-05 14:33:27,367 INFO ___FILE_ONLY___ ═
520
+ 2025-11-05 14:33:27,369 INFO ___FILE_ONLY___ ═
521
+ 2025-11-05 14:33:27,371 INFO ___FILE_ONLY___ ═
522
+ 2025-11-05 14:33:27,372 INFO ___FILE_ONLY___ ═
523
+ 2025-11-05 14:33:27,376 INFO ___FILE_ONLY___ ═
524
+ 2025-11-05 14:33:27,378 INFO ___FILE_ONLY___ ═
525
+ 2025-11-05 14:33:27,380 INFO ___FILE_ONLY___ ═
526
+ 2025-11-05 14:33:27,382 INFO ___FILE_ONLY___ ═
527
+ 2025-11-05 14:33:27,386 INFO ___FILE_ONLY___ ═
528
+ 2025-11-05 14:33:27,396 INFO ___FILE_ONLY___ ═
529
+ 2025-11-05 14:33:27,401 INFO ___FILE_ONLY___ ═
530
+ 2025-11-05 14:33:27,403 INFO ___FILE_ONLY___ ═
531
+ 2025-11-05 14:33:27,405 INFO ___FILE_ONLY___ ═
532
+ 2025-11-05 14:33:27,408 INFO ___FILE_ONLY___ ═
533
+ 2025-11-05 14:33:27,410 INFO ___FILE_ONLY___ ═
534
+ 2025-11-05 14:33:27,413 INFO ___FILE_ONLY___ ═
535
+ 2025-11-05 14:33:27,417 INFO ___FILE_ONLY___ ═
536
+ 2025-11-05 14:33:27,419 INFO ___FILE_ONLY___ ═
537
+ 2025-11-05 14:33:27,422 INFO ___FILE_ONLY___ ═
538
+ 2025-11-05 14:33:27,424 INFO ___FILE_ONLY___ ═
539
+ 2025-11-05 14:33:27,426 INFO ___FILE_ONLY___ ═
540
+ 2025-11-05 14:33:27,428 INFO ___FILE_ONLY___ ═
541
+ 2025-11-05 14:33:27,430 INFO ___FILE_ONLY___ ═
542
+ 2025-11-05 14:33:27,433 INFO ___FILE_ONLY___ ═
543
+ 2025-11-05 14:33:27,435 INFO ___FILE_ONLY___ ═
544
+ 2025-11-05 14:33:27,437 INFO ___FILE_ONLY___ ═
545
+ 2025-11-05 14:33:27,440 INFO ___FILE_ONLY___ ═
546
+ 2025-11-05 14:33:27,442 INFO ___FILE_ONLY___ ═
547
+ 2025-11-05 14:33:27,444 INFO ___FILE_ONLY___ ═
548
+ 2025-11-05 14:33:27,446 INFO ___FILE_ONLY___ ═
549
+ 2025-11-05 14:33:27,448 INFO ___FILE_ONLY___ ═
550
+ 2025-11-05 14:33:27,448 INFO ___FILE_ONLY___ ╝
551
+
552
+ 2025-11-05 14:33:27,456 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
553
+
554
+ 2025-11-05 14:33:27,456 INFO ___FILE_ONLY___ ╠═ Installing: BigQuery Command Line Tool (Platform Spec... ═╣
555
+
556
+ 2025-11-05 14:33:27,456 INFO ___FILE_ONLY___ ╚
557
+ 2025-11-05 14:33:27,457 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
558
+ 2025-11-05 14:33:27,457 INFO ___FILE_ONLY___ ╝
559
+
560
+ 2025-11-05 14:33:27,461 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
561
+
562
+ 2025-11-05 14:33:27,461 INFO ___FILE_ONLY___ ╠═ Installing: Bundled Python 3.13 ═╣
563
+
564
+ 2025-11-05 14:33:27,461 INFO ___FILE_ONLY___ ╚
565
+ 2025-11-05 14:33:27,464 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
566
+ 2025-11-05 14:33:27,464 INFO ___FILE_ONLY___ ╝
567
+
568
+ 2025-11-05 14:33:27,466 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
569
+
570
+ 2025-11-05 14:33:27,466 INFO ___FILE_ONLY___ ╠═ Installing: Bundled Python 3.13 (Platform Specific) ═╣
571
+
572
+ 2025-11-05 14:33:27,466 INFO ___FILE_ONLY___ ╚
573
+ 2025-11-05 14:33:30,570 INFO ___FILE_ONLY___ ═
574
+ 2025-11-05 14:33:30,584 INFO ___FILE_ONLY___ ═
575
+ 2025-11-05 14:33:30,598 INFO ___FILE_ONLY___ ═
576
+ 2025-11-05 14:33:32,918 INFO ___FILE_ONLY___ ═
577
+ 2025-11-05 14:33:32,941 INFO ___FILE_ONLY___ ═
578
+ 2025-11-05 14:33:32,968 INFO ___FILE_ONLY___ ═
579
+ 2025-11-05 14:33:32,986 INFO ___FILE_ONLY___ ═
580
+ 2025-11-05 14:33:33,014 INFO ___FILE_ONLY___ ═
581
+ 2025-11-05 14:33:33,031 INFO ___FILE_ONLY___ ═
582
+ 2025-11-05 14:33:33,124 INFO ___FILE_ONLY___ ═
583
+ 2025-11-05 14:33:33,141 INFO ___FILE_ONLY___ ═
584
+ 2025-11-05 14:33:33,157 INFO ___FILE_ONLY___ ═
585
+ 2025-11-05 14:33:33,188 INFO ___FILE_ONLY___ ═
586
+ 2025-11-05 14:33:33,204 INFO ___FILE_ONLY___ ═
587
+ 2025-11-05 14:33:33,219 INFO ___FILE_ONLY___ ═
588
+ 2025-11-05 14:33:33,232 INFO ___FILE_ONLY___ ═
589
+ 2025-11-05 14:33:33,248 INFO ___FILE_ONLY___ ═
590
+ 2025-11-05 14:33:33,263 INFO ___FILE_ONLY___ ═
591
+ 2025-11-05 14:33:33,279 INFO ___FILE_ONLY___ ═
592
+ 2025-11-05 14:33:33,295 INFO ___FILE_ONLY___ ═
593
+ 2025-11-05 14:33:33,310 INFO ___FILE_ONLY___ ═
594
+ 2025-11-05 14:33:33,329 INFO ___FILE_ONLY___ ═
595
+ 2025-11-05 14:33:33,344 INFO ___FILE_ONLY___ ═
596
+ 2025-11-05 14:33:33,492 INFO ___FILE_ONLY___ ═
597
+ 2025-11-05 14:33:33,970 INFO ___FILE_ONLY___ ═
598
+ 2025-11-05 14:33:33,994 INFO ___FILE_ONLY___ ═
599
+ 2025-11-05 14:33:34,039 INFO ___FILE_ONLY___ ═
600
+ 2025-11-05 14:33:34,051 INFO ___FILE_ONLY___ ═
601
+ 2025-11-05 14:33:34,062 INFO ___FILE_ONLY___ ═
602
+ 2025-11-05 14:33:34,074 INFO ___FILE_ONLY___ ═
603
+ 2025-11-05 14:33:34,085 INFO ___FILE_ONLY___ ═
604
+ 2025-11-05 14:33:34,098 INFO ___FILE_ONLY___ ═
605
+ 2025-11-05 14:33:34,110 INFO ___FILE_ONLY___ ═
606
+ 2025-11-05 14:33:34,121 INFO ___FILE_ONLY___ ═
607
+ 2025-11-05 14:33:34,133 INFO ___FILE_ONLY___ ═
608
+ 2025-11-05 14:33:34,145 INFO ___FILE_ONLY___ ═
609
+ 2025-11-05 14:33:34,156 INFO ___FILE_ONLY___ ═
610
+ 2025-11-05 14:33:34,168 INFO ___FILE_ONLY___ ═
611
+ 2025-11-05 14:33:34,180 INFO ___FILE_ONLY___ ═
612
+ 2025-11-05 14:33:34,191 INFO ___FILE_ONLY___ ═
613
+ 2025-11-05 14:33:34,203 INFO ___FILE_ONLY___ ═
614
+ 2025-11-05 14:33:34,216 INFO ___FILE_ONLY___ ═
615
+ 2025-11-05 14:33:34,228 INFO ___FILE_ONLY___ ═
616
+ 2025-11-05 14:33:34,239 INFO ___FILE_ONLY___ ═
617
+ 2025-11-05 14:33:34,251 INFO ___FILE_ONLY___ ═
618
+ 2025-11-05 14:33:34,263 INFO ___FILE_ONLY___ ═
619
+ 2025-11-05 14:33:34,275 INFO ___FILE_ONLY___ ═
620
+ 2025-11-05 14:33:34,286 INFO ___FILE_ONLY___ ═
621
+ 2025-11-05 14:33:34,298 INFO ___FILE_ONLY___ ═
622
+ 2025-11-05 14:33:34,310 INFO ___FILE_ONLY___ ═
623
+ 2025-11-05 14:33:34,321 INFO ___FILE_ONLY___ ═
624
+ 2025-11-05 14:33:34,333 INFO ___FILE_ONLY___ ═
625
+ 2025-11-05 14:33:34,345 INFO ___FILE_ONLY___ ═
626
+ 2025-11-05 14:33:34,357 INFO ___FILE_ONLY___ ═
627
+ 2025-11-05 14:33:34,369 INFO ___FILE_ONLY___ ═
628
+ 2025-11-05 14:33:34,381 INFO ___FILE_ONLY___ ═
629
+ 2025-11-05 14:33:34,393 INFO ___FILE_ONLY___ ═
630
+ 2025-11-05 14:33:34,405 INFO ___FILE_ONLY___ ═
631
+ 2025-11-05 14:33:34,417 INFO ___FILE_ONLY___ ═
632
+ 2025-11-05 14:33:34,429 INFO ___FILE_ONLY___ ═
633
+ 2025-11-05 14:33:34,429 INFO ___FILE_ONLY___ ╝
634
+
635
+ 2025-11-05 14:33:34,490 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
636
+
637
+ 2025-11-05 14:33:34,490 INFO ___FILE_ONLY___ ╠═ Installing: Cloud Storage Command Line Tool ═╣
638
+
639
+ 2025-11-05 14:33:34,490 INFO ___FILE_ONLY___ ╚
640
+ 2025-11-05 14:33:35,031 INFO ___FILE_ONLY___ ═
641
+ 2025-11-05 14:33:35,042 INFO ___FILE_ONLY___ ═
642
+ 2025-11-05 14:33:35,053 INFO ___FILE_ONLY___ ═
643
+ 2025-11-05 14:33:35,063 INFO ___FILE_ONLY___ ═
644
+ 2025-11-05 14:33:35,072 INFO ___FILE_ONLY___ ═
645
+ 2025-11-05 14:33:35,105 INFO ___FILE_ONLY___ ═
646
+ 2025-11-05 14:33:35,117 INFO ___FILE_ONLY___ ═
647
+ 2025-11-05 14:33:35,132 INFO ___FILE_ONLY___ ═
648
+ 2025-11-05 14:33:35,147 INFO ___FILE_ONLY___ ═
649
+ 2025-11-05 14:33:35,161 INFO ___FILE_ONLY___ ═
650
+ 2025-11-05 14:33:35,178 INFO ___FILE_ONLY___ ═
651
+ 2025-11-05 14:33:35,189 INFO ___FILE_ONLY___ ═
652
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653
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654
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655
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656
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657
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658
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659
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660
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661
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662
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663
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664
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665
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666
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667
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668
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669
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670
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671
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672
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673
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674
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675
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676
+ 2025-11-05 14:33:35,493 INFO ___FILE_ONLY___ ═
677
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678
+ 2025-11-05 14:33:35,538 INFO ___FILE_ONLY___ ═
679
+ 2025-11-05 14:33:35,555 INFO ___FILE_ONLY___ ═
680
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681
+ 2025-11-05 14:33:35,576 INFO ___FILE_ONLY___ ═
682
+ 2025-11-05 14:33:35,586 INFO ___FILE_ONLY___ ═
683
+ 2025-11-05 14:33:35,596 INFO ___FILE_ONLY___ ═
684
+ 2025-11-05 14:33:35,607 INFO ___FILE_ONLY___ ═
685
+ 2025-11-05 14:33:35,616 INFO ___FILE_ONLY___ ═
686
+ 2025-11-05 14:33:35,626 INFO ___FILE_ONLY___ ═
687
+ 2025-11-05 14:33:35,635 INFO ___FILE_ONLY___ ═
688
+ 2025-11-05 14:33:35,644 INFO ___FILE_ONLY___ ═
689
+ 2025-11-05 14:33:35,656 INFO ___FILE_ONLY___ ═
690
+ 2025-11-05 14:33:35,670 INFO ___FILE_ONLY___ ═
691
+ 2025-11-05 14:33:35,682 INFO ___FILE_ONLY___ ═
692
+ 2025-11-05 14:33:35,695 INFO ___FILE_ONLY___ ═
693
+ 2025-11-05 14:33:35,707 INFO ___FILE_ONLY___ ═
694
+ 2025-11-05 14:33:35,719 INFO ___FILE_ONLY___ ═
695
+ 2025-11-05 14:33:35,733 INFO ___FILE_ONLY___ ═
696
+ 2025-11-05 14:33:35,749 INFO ___FILE_ONLY___ ═
697
+ 2025-11-05 14:33:35,769 INFO ___FILE_ONLY___ ═
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+ 2025-11-05 14:33:35,785 INFO ___FILE_ONLY___ ═
699
+ 2025-11-05 14:33:35,802 INFO ___FILE_ONLY___ ═
700
+ 2025-11-05 14:33:35,802 INFO ___FILE_ONLY___ ╝
701
+
702
+ 2025-11-05 14:33:35,831 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
703
+
704
+ 2025-11-05 14:33:35,831 INFO ___FILE_ONLY___ ╠═ Installing: Cloud Storage Command Line Tool (Platform... ═╣
705
+
706
+ 2025-11-05 14:33:35,831 INFO ___FILE_ONLY___ ╚
707
+ 2025-11-05 14:33:35,832 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
708
+ 2025-11-05 14:33:35,832 INFO ___FILE_ONLY___ ╝
709
+
710
+ 2025-11-05 14:33:35,836 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
711
+
712
+ 2025-11-05 14:33:35,836 INFO ___FILE_ONLY___ ╠═ Installing: Default set of gcloud commands ═╣
713
+
714
+ 2025-11-05 14:33:35,836 INFO ___FILE_ONLY___ ╚
715
+ 2025-11-05 14:33:35,838 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
716
+ 2025-11-05 14:33:35,839 INFO ___FILE_ONLY___ ╝
717
+
718
+ 2025-11-05 14:33:35,840 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
719
+
720
+ 2025-11-05 14:33:35,840 INFO ___FILE_ONLY___ ╠═ Installing: Google Cloud CLI Core Libraries (Platform... ═╣
721
+
722
+ 2025-11-05 14:33:35,840 INFO ___FILE_ONLY___ ╚
723
+ 2025-11-05 14:33:35,841 INFO ___FILE_ONLY___ ══════════════════════════════
724
+ 2025-11-05 14:33:35,841 INFO ___FILE_ONLY___ ══════════════════════════════
725
+ 2025-11-05 14:33:35,841 INFO ___FILE_ONLY___ ╝
726
+
727
+ 2025-11-05 14:33:35,845 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
728
+
729
+ 2025-11-05 14:33:35,845 INFO ___FILE_ONLY___ ╠═ Installing: Google Cloud CRC32C Hash Tool ═╣
730
+
731
+ 2025-11-05 14:33:35,845 INFO ___FILE_ONLY___ ╚
732
+ 2025-11-05 14:33:35,847 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
733
+ 2025-11-05 14:33:35,847 INFO ___FILE_ONLY___ ╝
734
+
735
+ 2025-11-05 14:33:35,849 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
736
+
737
+ 2025-11-05 14:33:35,849 INFO ___FILE_ONLY___ ╠═ Installing: Google Cloud CRC32C Hash Tool (Platform S... ═╣
738
+
739
+ 2025-11-05 14:33:35,849 INFO ___FILE_ONLY___ ╚
740
+ 2025-11-05 14:33:35,884 INFO ___FILE_ONLY___ ══════════════════════════════
741
+ 2025-11-05 14:33:35,884 INFO ___FILE_ONLY___ ══════════════════════════════
742
+ 2025-11-05 14:33:35,884 INFO ___FILE_ONLY___ ╝
743
+
744
+ 2025-11-05 14:33:35,889 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
745
+
746
+ 2025-11-05 14:33:35,889 INFO ___FILE_ONLY___ ╠═ Installing: gcloud cli dependencies (Platform Specific) ═╣
747
+
748
+ 2025-11-05 14:33:35,889 INFO ___FILE_ONLY___ ╚
749
+ 2025-11-05 14:33:35,889 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
750
+ 2025-11-05 14:33:35,889 INFO ___FILE_ONLY___ ╝
751
+
752
+ 2025-11-05 14:33:35,894 DEBUG root Updating notification cache...
753
+ 2025-11-05 14:33:35,894 INFO ___FILE_ONLY___
754
+
755
+ 2025-11-05 14:33:35,895 INFO ___FILE_ONLY___ Performing post processing steps...
756
+ 2025-11-05 14:33:35,896 DEBUG root Executing command: ['/tools/google-cloud-sdk/bin/gcloud', 'components', 'post-process']
757
+ 2025-11-05 14:33:43,462 DEBUG ___FILE_ONLY___
758
+ 2025-11-05 14:33:43,462 DEBUG ___FILE_ONLY___
759
+ 2025-11-05 14:33:43,685 INFO root descriptor_list: [{'universeDomain': 'googleapis.com', 'universeShortName': '', 'authenticationDomain': 'auth.cloud.google.com', 'projectPrefix': '', 'cloudWebDomain': 'cloud.google.com', 'documentationDomain': 'cloud.google.com', 'version': '1.0.0', 'state': 'primary', 'artifactRegistryDomain': 'pkg.dev'}]
760
+ 2025-11-05 14:33:43,685 INFO ___FILE_ONLY___
761
+ Update done!
762
+
763
+
764
+ 2025-11-05 14:33:43,688 DEBUG root Chosen display Format:none
765
+ 2025-11-05 14:33:43,688 INFO root Display format: "none"
.config/logs/2025.11.05/14.33.36.385956.log ADDED
@@ -0,0 +1,5 @@
 
 
 
 
 
 
1
+ 2025-11-05 14:33:36,386 DEBUG root Loaded Command Group: ['gcloud', 'components']
2
+ 2025-11-05 14:33:36,388 DEBUG root Loaded Command Group: ['gcloud', 'components', 'post_process']
3
+ 2025-11-05 14:33:36,390 DEBUG root Running [gcloud.components.post-process] with arguments: []
4
+ 2025-11-05 14:33:43,370 DEBUG root Chosen display Format:none
5
+ 2025-11-05 14:33:43,370 INFO root Display format: "none"
.config/logs/2025.11.05/14.33.44.287731.log ADDED
@@ -0,0 +1,153 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ 2025-11-05 14:33:44,288 DEBUG root Loaded Command Group: ['gcloud', 'components']
2
+ 2025-11-05 14:33:44,320 DEBUG root Loaded Command Group: ['gcloud', 'components', 'update']
3
+ 2025-11-05 14:33:44,322 DEBUG root Running [gcloud.components.update] with arguments: [--quiet: "True", COMPONENT-IDS:8: "['gcloud', 'core', 'bq', 'gsutil', 'compute', 'preview', 'alpha', 'beta']"]
4
+ 2025-11-05 14:33:44,323 INFO ___FILE_ONLY___ Beginning update. This process may take several minutes.
5
+
6
+ 2025-11-05 14:33:44,330 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): dl.google.com:443
7
+ 2025-11-05 14:33:44,342 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/components-2.json HTTP/1.1" 200 233158
8
+ 2025-11-05 14:33:44,355 WARNING root Component [compute] no longer exists.
9
+ 2025-11-05 14:33:44,356 INFO ___FILE_ONLY___
10
+
11
+ 2025-11-05 14:33:44,356 INFO ___FILE_ONLY___
12
+ Your current Google Cloud CLI version is: 546.0.0
13
+
14
+ 2025-11-05 14:33:44,356 INFO ___FILE_ONLY___ Installing components from version: 546.0.0
15
+
16
+ 2025-11-05 14:33:44,356 INFO ___FILE_ONLY___
17
+
18
+ 2025-11-05 14:33:44,356 DEBUG root Chosen display Format:table[box,title="These components will be removed."](details.display_name:label=Name:align=left,version.version_string:label=Version:align=right,data.size.size(zero="",min=1048576):label=Size:align=right)
19
+ 2025-11-05 14:33:44,357 DEBUG root Chosen display Format:table[box,title="These components will be updated."](details.display_name:label=Name:align=left,version.version_string:label=Version:align=right,data.size.size(zero="",min=1048576):label=Size:align=right)
20
+ 2025-11-05 14:33:44,357 DEBUG root Chosen display Format:table[box,title="These components will be installed."](details.display_name:label=Name:align=left,version.version_string:label=Version:align=right,data.size.size(zero="",min=1048576):label=Size:align=right)
21
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ ┌────────────────────────────────────────────────┐
22
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___
23
+
24
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ │ These components will be installed. │
25
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___
26
+
27
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ ├─────────────────────────┬────────────┬─────────┤
28
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___
29
+
30
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ │ Name │ Version │ Size │
31
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___
32
+
33
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ ├─────────────────────────┼────────────┼─────────┤
34
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___
35
+
36
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ │
37
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ gcloud Alpha Commands
38
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___
39
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ │
40
+ 2025-11-05 14:33:44,373 INFO ___FILE_ONLY___ 2025.10.31
41
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___
42
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ │
43
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ < 1 MiB
44
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___
45
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ │
46
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___
47
+
48
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ │
49
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ gcloud Beta Commands
50
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___
51
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ │
52
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ 2025.10.31
53
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___
54
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ │
55
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ < 1 MiB
56
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___
57
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ │
58
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___
59
+
60
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ │
61
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___ gcloud Preview Commands
62
+ 2025-11-05 14:33:44,374 INFO ___FILE_ONLY___
63
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___ │
64
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___
65
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___
66
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___ │
67
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___ < 1 MiB
68
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___
69
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___ │
70
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___
71
+
72
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___ └─────────────────────────┴──��─────────┴─────────┘
73
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___
74
+
75
+ 2025-11-05 14:33:44,375 INFO ___FILE_ONLY___
76
+
77
+ 2025-11-05 14:33:44,378 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): dl.google.com:443
78
+ 2025-11-05 14:33:44,388 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/RELEASE_NOTES HTTP/1.1" 200 1514051
79
+ 2025-11-05 14:33:44,913 INFO ___FILE_ONLY___ For the latest full release notes, please visit:
80
+ https://cloud.google.com/sdk/release_notes
81
+
82
+
83
+ 2025-11-05 14:33:44,914 INFO ___FILE_ONLY___ Performing in place update...
84
+
85
+
86
+ 2025-11-05 14:33:44,915 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
87
+
88
+ 2025-11-05 14:33:44,916 INFO ___FILE_ONLY___ ╠═ Downloading: gcloud Alpha Commands ═╣
89
+
90
+ 2025-11-05 14:33:44,916 INFO ___FILE_ONLY___ ╚
91
+ 2025-11-05 14:33:44,918 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): dl.google.com:443
92
+ 2025-11-05 14:33:44,970 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/components/google-cloud-sdk-alpha-20251031202127.tar.gz HTTP/1.1" 200 800
93
+ 2025-11-05 14:33:44,970 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
94
+ 2025-11-05 14:33:44,970 INFO ___FILE_ONLY___ ╝
95
+
96
+ 2025-11-05 14:33:44,972 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
97
+
98
+ 2025-11-05 14:33:44,972 INFO ___FILE_ONLY___ ╠═ Downloading: gcloud Beta Commands ═╣
99
+
100
+ 2025-11-05 14:33:44,972 INFO ___FILE_ONLY___ ╚
101
+ 2025-11-05 14:33:44,975 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): dl.google.com:443
102
+ 2025-11-05 14:33:44,987 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/components/google-cloud-sdk-beta-20251031202127.tar.gz HTTP/1.1" 200 797
103
+ 2025-11-05 14:33:44,988 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
104
+ 2025-11-05 14:33:44,988 INFO ___FILE_ONLY___ ╝
105
+
106
+ 2025-11-05 14:33:44,989 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
107
+
108
+ 2025-11-05 14:33:44,989 INFO ___FILE_ONLY___ ╠═ Downloading: gcloud Preview Commands ═╣
109
+
110
+ 2025-11-05 14:33:44,990 INFO ___FILE_ONLY___ ╚
111
+ 2025-11-05 14:33:44,992 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): dl.google.com:443
112
+ 2025-11-05 14:33:45,050 DEBUG urllib3.connectionpool https://dl.google.com:443 "GET /dl/cloudsdk/channels/rapid/components/google-cloud-sdk-preview-20241115154308.tar.gz HTTP/1.1" 200 823
113
+ 2025-11-05 14:33:45,051 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
114
+ 2025-11-05 14:33:45,051 INFO ___FILE_ONLY___ ╝
115
+
116
+ 2025-11-05 14:33:45,053 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
117
+
118
+ 2025-11-05 14:33:45,053 INFO ___FILE_ONLY___ ╠═ Installing: gcloud Alpha Commands ═╣
119
+
120
+ 2025-11-05 14:33:45,053 INFO ___FILE_ONLY___ ╚
121
+ 2025-11-05 14:33:45,054 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
122
+ 2025-11-05 14:33:45,054 INFO ___FILE_ONLY___ ╝
123
+
124
+ 2025-11-05 14:33:45,058 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
125
+
126
+ 2025-11-05 14:33:45,058 INFO ___FILE_ONLY___ ╠═ Installing: gcloud Beta Commands ═╣
127
+
128
+ 2025-11-05 14:33:45,058 INFO ___FILE_ONLY___ ╚
129
+ 2025-11-05 14:33:45,059 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
130
+ 2025-11-05 14:33:45,059 INFO ___FILE_ONLY___ ╝
131
+
132
+ 2025-11-05 14:33:45,064 INFO ___FILE_ONLY___ ╔════════════════════════════════════════════════════════════╗
133
+
134
+ 2025-11-05 14:33:45,064 INFO ___FILE_ONLY___ ╠═ Installing: gcloud Preview Commands ═╣
135
+
136
+ 2025-11-05 14:33:45,064 INFO ___FILE_ONLY___ ╚
137
+ 2025-11-05 14:33:45,065 INFO ___FILE_ONLY___ ════════════════════════════════════════════════════════════
138
+ 2025-11-05 14:33:45,065 INFO ___FILE_ONLY___ ╝
139
+
140
+ 2025-11-05 14:33:45,070 DEBUG root Updating notification cache...
141
+ 2025-11-05 14:33:45,070 INFO ___FILE_ONLY___
142
+
143
+ 2025-11-05 14:33:45,071 INFO ___FILE_ONLY___ Performing post processing steps...
144
+ 2025-11-05 14:33:45,072 DEBUG root Executing command: ['/tools/google-cloud-sdk/bin/gcloud', 'components', 'post-process']
145
+ 2025-11-05 14:33:52,779 DEBUG ___FILE_ONLY___
146
+ 2025-11-05 14:33:52,779 DEBUG ___FILE_ONLY___
147
+ 2025-11-05 14:33:52,846 INFO root descriptor_list: [{'universeDomain': 'googleapis.com', 'universeShortName': '', 'authenticationDomain': 'auth.cloud.google.com', 'projectPrefix': '', 'cloudWebDomain': 'cloud.google.com', 'documentationDomain': 'cloud.google.com', 'version': '1.0.0', 'state': 'primary', 'artifactRegistryDomain': 'pkg.dev'}]
148
+ 2025-11-05 14:33:52,846 INFO ___FILE_ONLY___
149
+ Update done!
150
+
151
+
152
+ 2025-11-05 14:33:52,848 DEBUG root Chosen display Format:none
153
+ 2025-11-05 14:33:52,849 INFO root Display format: "none"
.config/logs/2025.11.05/14.33.45.559498.log ADDED
@@ -0,0 +1,5 @@
 
 
 
 
 
 
1
+ 2025-11-05 14:33:45,560 DEBUG root Loaded Command Group: ['gcloud', 'components']
2
+ 2025-11-05 14:33:45,561 DEBUG root Loaded Command Group: ['gcloud', 'components', 'post_process']
3
+ 2025-11-05 14:33:45,563 DEBUG root Running [gcloud.components.post-process] with arguments: []
4
+ 2025-11-05 14:33:52,685 DEBUG root Chosen display Format:none
5
+ 2025-11-05 14:33:52,686 INFO root Display format: "none"
.config/logs/2025.11.05/14.33.53.434728.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-05 14:33:53,436 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-05 14:33:53,521 DEBUG root Loaded Command Group: ['gcloud', 'config', 'set']
3
+ 2025-11-05 14:33:53,523 DEBUG root Running [gcloud.config.set] with arguments: [SECTION/PROPERTY: "component_manager/disable_update_check", VALUE: "true"]
4
+ 2025-11-05 14:33:53,524 INFO ___FILE_ONLY___ Updated property [component_manager/disable_update_check].
5
+
6
+ 2025-11-05 14:33:53,525 DEBUG root Chosen display Format:default
7
+ 2025-11-05 14:33:53,525 INFO root Display format: "default"
8
+ 2025-11-05 14:33:53,530 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.05/14.33.54.129583.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-05 14:33:54,131 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-05 14:33:54,216 DEBUG root Loaded Command Group: ['gcloud', 'config', 'set']
3
+ 2025-11-05 14:33:54,218 DEBUG root Running [gcloud.config.set] with arguments: [SECTION/PROPERTY: "compute/gce_metadata_read_timeout_sec", VALUE: "0"]
4
+ 2025-11-05 14:33:54,219 INFO ___FILE_ONLY___ Updated property [compute/gce_metadata_read_timeout_sec].
5
+
6
+ 2025-11-05 14:33:54,219 DEBUG root Chosen display Format:default
7
+ 2025-11-05 14:33:54,220 INFO root Display format: "default"
8
+ 2025-11-05 14:33:54,225 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.31.43.532264.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:31:43,534 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 03:31:43,545 DEBUG root Loaded Command Group: ['gcloud', 'config', 'unset']
3
+ 2025-11-08 03:31:43,547 DEBUG root Running [gcloud.config.unset] with arguments: [SECTION/PROPERTY: "compute/gce_metadata_read_timeout_sec"]
4
+ 2025-11-08 03:31:43,547 INFO ___FILE_ONLY___ Unset property [compute/gce_metadata_read_timeout_sec].
5
+
6
+ 2025-11-08 03:31:43,548 DEBUG root Chosen display Format:default
7
+ 2025-11-08 03:31:43,549 INFO root Display format: "default"
8
+ 2025-11-08 03:31:43,603 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.38.30.158621.log ADDED
@@ -0,0 +1,18 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:38:30,167 DEBUG root Loaded Command Group: ['gcloud', 'auth']
2
+ 2025-11-08 03:38:30,304 DEBUG root Loaded Command Group: ['gcloud', 'auth', 'revoke']
3
+ 2025-11-08 03:38:30,311 DEBUG root Running [gcloud.auth.revoke] with arguments: [--all: "True", --quiet: "True"]
4
+ 2025-11-08 03:38:32,958 DEBUG root (gcloud.auth.revoke) Cannot revoke GCE-provided credentials.
5
+ Traceback (most recent call last):
6
+ File "/tools/google-cloud-sdk/lib/googlecloudsdk/calliope/cli.py", line 944, in Execute
7
+ resources = calliope_command.Run(cli=self, args=args)
8
+ ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
9
+ File "/tools/google-cloud-sdk/lib/googlecloudsdk/calliope/backend.py", line 955, in Run
10
+ resources = command_instance.Run(args)
11
+ ^^^^^^^^^^^^^^^^^^^^^^^^^^
12
+ File "/tools/google-cloud-sdk/lib/surface/auth/revoke.py", line 118, in Run
13
+ if not c_store.Revoke(account):
14
+ ^^^^^^^^^^^^^^^^^^^^^^^
15
+ File "/tools/google-cloud-sdk/lib/googlecloudsdk/core/credentials/store.py", line 1698, in Revoke
16
+ raise RevokeError('Cannot revoke GCE-provided credentials.')
17
+ googlecloudsdk.core.credentials.store.RevokeError: Cannot revoke GCE-provided credentials.
18
+ 2025-11-08 03:38:32,963 ERROR root (gcloud.auth.revoke) Cannot revoke GCE-provided credentials.
.config/logs/2025.11.08/03.38.33.972101.log ADDED
@@ -0,0 +1,9 @@
 
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:38:33,973 DEBUG root Loaded Command Group: ['gcloud', 'auth']
2
+ 2025-11-08 03:38:33,982 DEBUG root Loaded Command Group: ['gcloud', 'auth', 'application_default']
3
+ 2025-11-08 03:38:33,998 DEBUG root Loaded Command Group: ['gcloud', 'auth', 'application-default', 'revoke']
4
+ 2025-11-08 03:38:34,000 DEBUG root Running [gcloud.auth.application-default.revoke] with arguments: [--quiet: "True"]
5
+ 2025-11-08 03:38:34,001 INFO ___FILE_ONLY___ Application Default Credentials have not been set up, nothing to revoke.
6
+
7
+ 2025-11-08 03:38:34,001 DEBUG root Chosen display Format:none
8
+ 2025-11-08 03:38:34,001 INFO root Display format: "none"
9
+ 2025-11-08 03:38:34,098 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.41.52.318962.log ADDED
@@ -0,0 +1,30 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:41:52,319 DEBUG root Loaded Command Group: ['gcloud', 'auth']
2
+ 2025-11-08 03:41:52,326 DEBUG root Loaded Command Group: ['gcloud', 'auth', 'list']
3
+ 2025-11-08 03:41:52,331 DEBUG root Running [gcloud.auth.list] with arguments: []
4
+ 2025-11-08 03:41:53,628 DEBUG root Chosen display Format: table[title='Credentialed Accounts'](
5
+ status.yesno(yes='*', no=''):label=ACTIVE,
6
+ account
7
+ )
8
+ 2025-11-08 03:41:53,629 INFO root Display format: " table[title='Credentialed Accounts'](
9
+ status.yesno(yes='*', no=''):label=ACTIVE,
10
+ account
11
+ )"
12
+ 2025-11-08 03:41:53,725 INFO ___FILE_ONLY___ Credentialed Accounts
13
+ 2025-11-08 03:41:53,725 INFO ___FILE_ONLY___
14
+
15
+ 2025-11-08 03:41:53,725 INFO ___FILE_ONLY___ ACTIVE
16
+ 2025-11-08 03:41:53,725 INFO ___FILE_ONLY___
17
+ 2025-11-08 03:41:53,725 INFO ___FILE_ONLY___ ACCOUNT
18
+ 2025-11-08 03:41:53,725 INFO ___FILE_ONLY___
19
+
20
+ 2025-11-08 03:41:53,725 INFO ___FILE_ONLY___ *
21
+ 2025-11-08 03:41:53,725 INFO ___FILE_ONLY___
22
+ 2025-11-08 03:41:53,726 INFO ___FILE_ONLY___ yiqu.rising@gmail.com
23
+ 2025-11-08 03:41:53,726 INFO ___FILE_ONLY___
24
+
25
+ 2025-11-08 03:41:53,726 INFO ___FILE_ONLY___
26
+ To set the active account, run:
27
+ $ gcloud config set account `ACCOUNT`
28
+
29
+
30
+ 2025-11-08 03:41:53,739 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.41.54.648119.log ADDED
@@ -0,0 +1,39 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:41:54,649 DEBUG root Loaded Command Group: ['gcloud', 'auth']
2
+ 2025-11-08 03:41:54,678 DEBUG root Loaded Command Group: ['gcloud', 'auth', 'login']
3
+ 2025-11-08 03:41:54,681 DEBUG root Running [gcloud.auth.login] with arguments: [--update-adc: "True"]
4
+ 2025-11-08 03:42:47,637 DEBUG requests_oauthlib.oauth2_session Generated new state UbZpE7wyHhiHeLrISJV4dlQhQqRsUW.
5
+ 2025-11-08 03:42:47,637 INFO ___FILE_ONLY___ Go to the following link in your browser, and complete the sign-in prompts:
6
+
7
+ https://accounts.google.com/o/oauth2/auth?response_type=code&client_id=32555940559.apps.googleusercontent.com&redirect_uri=https%3A%2F%2Fsdk.cloud.google.com%2Fauthcode.html&scope=openid+https%3A%2F%2Fwww.googleapis.com%2Fauth%2Fuserinfo.email+https%3A%2F%2Fwww.googleapis.com%2Fauth%2Fcloud-platform+https%3A%2F%2Fwww.googleapis.com%2Fauth%2Fappengine.admin+https%3A%2F%2Fwww.googleapis.com%2Fauth%2Fsqlservice.login+https%3A%2F%2Fwww.googleapis.com%2Fauth%2Fcompute+https%3A%2F%2Fwww.googleapis.com%2Fauth%2Faccounts.reauth&state=UbZpE7wyHhiHeLrISJV4dlQhQqRsUW&prompt=consent&token_usage=remote&access_type=offline&code_challenge=maf8MoTx0k-wqonpf7L-H_edqJAhfdL1vqWdYIt2fYI&code_challenge_method=S256
8
+
9
+
10
+ 2025-11-08 03:43:40,193 DEBUG requests_oauthlib.oauth2_session Requesting url https://oauth2.googleapis.com/token using method POST.
11
+ 2025-11-08 03:43:40,193 DEBUG requests_oauthlib.oauth2_session Supplying headers {b'Accept': b'application/json', b'Content-Type': b'application/x-www-form-urlencoded;charset=UTF-8', b'user-agent': b'google-cloud-sdk gcloud/546.0.0 command/gcloud.auth.login invocation-id/c377213330524b4fbb7c6f419374122a environment/GCE environment-version/None client-os/LINUX client-os-ver/6.6.105 client-pltf-arch/x86_64 interactive/True from-script/True python/3.12.12 term/xterm-color (Linux 6.6.105+)'} and data {'grant_type': 'authorization_code', 'client_id': '32555940559.apps.googleusercontent.com', 'client_secret': 'ZmssLNjJy2998hD4CTg2ejr2', 'code': '4/0Ab32j914uyaD3W_ztbjGemOJsEVAW7GOpH2bLvNbb5-ru0sy5Uf3XDTsQ7BhVdeGKDqY_A', 'redirect_uri': 'https://sdk.cloud.google.com/authcode.html', 'code_verifier': 'j3VMl54_cdjOt_ZApbhATZUtNXTlKho4AMH0Mh6FULJOKTxOuJahlSnS.5l7Xz5T__lcqjuzPi0iDQZDDDhTz16eeFWfVs5yeV7g2l9Yw9UkOFsGKh6PfVuzouf12NMz'}
12
+ 2025-11-08 03:43:40,193 DEBUG requests_oauthlib.oauth2_session Passing through key word arguments {'json': None, 'timeout': None, 'auth': None, 'verify': None, 'proxies': None}.
13
+ 2025-11-08 03:43:40,194 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): oauth2.googleapis.com:443
14
+ 2025-11-08 03:43:40,243 DEBUG urllib3.connectionpool https://oauth2.googleapis.com:443 "POST /token HTTP/1.1" 200 None
15
+ 2025-11-08 03:43:40,243 DEBUG requests_oauthlib.oauth2_session Prepared fetch token request body grant_type=authorization_code&client_id=32555940559.apps.googleusercontent.com&client_secret=ZmssLNjJy2998hD4CTg2ejr2&code=4%2F0Ab32j914uyaD3W_ztbjGemOJsEVAW7GOpH2bLvNbb5-ru0sy5Uf3XDTsQ7BhVdeGKDqY_A&redirect_uri=https%3A%2F%2Fsdk.cloud.google.com%2Fauthcode.html&code_verifier=j3VMl54_cdjOt_ZApbhATZUtNXTlKho4AMH0Mh6FULJOKTxOuJahlSnS.5l7Xz5T__lcqjuzPi0iDQZDDDhTz16eeFWfVs5yeV7g2l9Yw9UkOFsGKh6PfVuzouf12NMz
16
+ 2025-11-08 03:43:40,243 DEBUG requests_oauthlib.oauth2_session Request to fetch token completed with status 200.
17
+ 2025-11-08 03:43:40,243 DEBUG requests_oauthlib.oauth2_session Request headers were {'user-agent': b'google-cloud-sdk gcloud/546.0.0 command/gcloud.auth.login invocation-id/c377213330524b4fbb7c6f419374122a environment/GCE environment-version/None client-os/LINUX client-os-ver/6.6.105 client-pltf-arch/x86_64 interactive/True from-script/True python/3.12.12 term/xterm-color (Linux 6.6.105+)', 'Accept-Encoding': 'gzip, deflate', 'Accept': b'application/json', 'Connection': 'keep-alive', 'Content-Type': b'application/x-www-form-urlencoded;charset=UTF-8', 'Content-Length': '405'}
18
+ 2025-11-08 03:43:40,243 DEBUG requests_oauthlib.oauth2_session Request body was grant_type=authorization_code&client_id=32555940559.apps.googleusercontent.com&client_secret=ZmssLNjJy2998hD4CTg2ejr2&code=4%2F0Ab32j914uyaD3W_ztbjGemOJsEVAW7GOpH2bLvNbb5-ru0sy5Uf3XDTsQ7BhVdeGKDqY_A&redirect_uri=https%3A%2F%2Fsdk.cloud.google.com%2Fauthcode.html&code_verifier=j3VMl54_cdjOt_ZApbhATZUtNXTlKho4AMH0Mh6FULJOKTxOuJahlSnS.5l7Xz5T__lcqjuzPi0iDQZDDDhTz16eeFWfVs5yeV7g2l9Yw9UkOFsGKh6PfVuzouf12NMz
19
+ 2025-11-08 03:43:40,244 DEBUG requests_oauthlib.oauth2_session Response headers were {'Pragma': 'no-cache', 'Expires': 'Mon, 01 Jan 1990 00:00:00 GMT', 'Date': 'Sat, 08 Nov 2025 03:43:40 GMT', 'Cache-Control': 'no-cache, no-store, max-age=0, must-revalidate', 'Content-Type': 'application/json; charset=utf-8', 'Vary': 'Origin, X-Origin, Referer', 'Content-Encoding': 'gzip', 'Server': 'scaffolding on HTTPServer2', 'X-XSS-Protection': '0', 'X-Frame-Options': 'SAMEORIGIN', 'X-Content-Type-Options': 'nosniff', 'Transfer-Encoding': 'chunked'} and content {
20
+ "access_token": "ya29.a0ATi6K2so5RCSo2fT8t7_GYxzm5yTF77mIqOsNtzC9iCWmxK89kI-MnJ03GNGwj6kLn18UjivfNHt5nLcHC3Z-ij6IvXg-iFYDQqS7RFowsswhcURpDawzdz_rN2iQLbX8RDelLBPvUcXOfjZWuJyii4MUrgjIMfpeeA0K4l8Ev8vu3ZBfA8_gRV3Ehtqx7GuPzMuBpq4HttHaCgYKAaQSARESFQHGX2MiXYeXS67caKV15ve02LPIMA0211",
21
+ "expires_in": 3599,
22
+ "refresh_token": "1//0gnRqTAeK7oNbCgYIARAAGBASNwF-L9IrqqKn5MpcjUzYnUJ0lrWZTF9qqdbnsK-ZL5lF0FsYsOzJirJwBsSRFGTfwAO-9_fqiMg",
23
+ "scope": "https://www.googleapis.com/auth/accounts.reauth https://www.googleapis.com/auth/compute https://www.googleapis.com/auth/sqlservice.login https://www.googleapis.com/auth/userinfo.email openid https://www.googleapis.com/auth/appengine.admin https://www.googleapis.com/auth/cloud-platform",
24
+ "token_type": "Bearer",
25
+ "id_token": "eyJhbGciOiJSUzI1NiIsImtpZCI6ImI1ZTQ0MGFlOTQxZTk5ODFlZTJmYTEzNzZkNDJjNDZkNzMxZGVlM2YiLCJ0eXAiOiJKV1QifQ.eyJpc3MiOiJodHRwczovL2FjY291bnRzLmdvb2dsZS5jb20iLCJhenAiOiIzMjU1NTk0MDU1OS5hcHBzLmdvb2dsZXVzZXJjb250ZW50LmNvbSIsImF1ZCI6IjMyNTU1OTQwNTU5LmFwcHMuZ29vZ2xldXNlcmNvbnRlbnQuY29tIiwic3ViIjoiMTAwNjc4ODk5Mzk2MzIzMzEwMjI4IiwiZW1haWwiOiJ5aXF1LnJpc2luZ0BnbWFpbC5jb20iLCJlbWFpbF92ZXJpZmllZCI6dHJ1ZSwiYXRfaGFzaCI6InRkWlp5WmhxcklUYXNjVlQ2MU96R0EiLCJpYXQiOjE3NjI1NzM0MjAsImV4cCI6MTc2MjU3NzAyMH0.NYntoKpsR-WsCZqwfINuQlGS9xWyUYECyfzoFN7iyG3oA4jTUVNnfa9CArhV2k2S_fCQeySO_XN-olSLotaYksc_8weG_dHLt6RWNDC_p1IXiF1X-4TFNBOpp1mp6YtR1PkoyX7TTAEHwRuBaImDuyu7cpOD2k8wGsBQaH7puaemgRvf4xRPJMBaq4BBOk7K2qQsKY-yLEeZd5or6QPtdLPRSgDv0NdfWHtZtcDBzwgj95XZPoerCDFsI2yTmCsHk3hdi53JU2a1ctj2I5MoC5ZReEfur-t2Yx-MTG8TtneFw6TcIxCC6bUFus3IdjrTeJyysVANENU_1omN_Iqu2A"
26
+ }.
27
+ 2025-11-08 03:43:40,244 DEBUG requests_oauthlib.oauth2_session Invoking 0 token response hooks.
28
+ 2025-11-08 03:43:40,244 DEBUG requests_oauthlib.oauth2_session Obtained token {'access_token': 'ya29.a0ATi6K2so5RCSo2fT8t7_GYxzm5yTF77mIqOsNtzC9iCWmxK89kI-MnJ03GNGwj6kLn18UjivfNHt5nLcHC3Z-ij6IvXg-iFYDQqS7RFowsswhcURpDawzdz_rN2iQLbX8RDelLBPvUcXOfjZWuJyii4MUrgjIMfpeeA0K4l8Ev8vu3ZBfA8_gRV3Ehtqx7GuPzMuBpq4HttHaCgYKAaQSARESFQHGX2MiXYeXS67caKV15ve02LPIMA0211', 'expires_in': 3599, 'refresh_token': '1//0gnRqTAeK7oNbCgYIARAAGBASNwF-L9IrqqKn5MpcjUzYnUJ0lrWZTF9qqdbnsK-ZL5lF0FsYsOzJirJwBsSRFGTfwAO-9_fqiMg', 'scope': ['https://www.googleapis.com/auth/accounts.reauth', 'https://www.googleapis.com/auth/compute', 'https://www.googleapis.com/auth/sqlservice.login', 'https://www.googleapis.com/auth/userinfo.email', 'openid', 'https://www.googleapis.com/auth/appengine.admin', 'https://www.googleapis.com/auth/cloud-platform'], 'token_type': 'Bearer', 'id_token': 'eyJhbGciOiJSUzI1NiIsImtpZCI6ImI1ZTQ0MGFlOTQxZTk5ODFlZTJmYTEzNzZkNDJjNDZkNzMxZGVlM2YiLCJ0eXAiOiJKV1QifQ.eyJpc3MiOiJodHRwczovL2FjY291bnRzLmdvb2dsZS5jb20iLCJhenAiOiIzMjU1NTk0MDU1OS5hcHBzLmdvb2dsZXVzZXJjb250ZW50LmNvbSIsImF1ZCI6IjMyNTU1OTQwNTU5LmFwcHMuZ29vZ2xldXNlcmNvbnRlbnQuY29tIiwic3ViIjoiMTAwNjc4ODk5Mzk2MzIzMzEwMjI4IiwiZW1haWwiOiJ5aXF1LnJpc2luZ0BnbWFpbC5jb20iLCJlbWFpbF92ZXJpZmllZCI6dHJ1ZSwiYXRfaGFzaCI6InRkWlp5WmhxcklUYXNjVlQ2MU96R0EiLCJpYXQiOjE3NjI1NzM0MjAsImV4cCI6MTc2MjU3NzAyMH0.NYntoKpsR-WsCZqwfINuQlGS9xWyUYECyfzoFN7iyG3oA4jTUVNnfa9CArhV2k2S_fCQeySO_XN-olSLotaYksc_8weG_dHLt6RWNDC_p1IXiF1X-4TFNBOpp1mp6YtR1PkoyX7TTAEHwRuBaImDuyu7cpOD2k8wGsBQaH7puaemgRvf4xRPJMBaq4BBOk7K2qQsKY-yLEeZd5or6QPtdLPRSgDv0NdfWHtZtcDBzwgj95XZPoerCDFsI2yTmCsHk3hdi53JU2a1ctj2I5MoC5ZReEfur-t2Yx-MTG8TtneFw6TcIxCC6bUFus3IdjrTeJyysVANENU_1omN_Iqu2A', 'expires_at': 1762577019.2441561}.
29
+ 2025-11-08 03:43:40,265 INFO ___FILE_ONLY___
30
+ Application Default Credentials (ADC) were updated.
31
+
32
+ 2025-11-08 03:43:40,269 INFO ___FILE_ONLY___
33
+ You are now logged in as [yiqu.rising@gmail.com].
34
+ Your current project is [None]. You can change this setting by running:
35
+ $ gcloud config set project PROJECT_ID
36
+
37
+ 2025-11-08 03:43:40,270 DEBUG root Chosen display Format:none
38
+ 2025-11-08 03:43:40,270 INFO root Display format: "none"
39
+ 2025-11-08 03:43:40,287 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.46.31.985987.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:46:31,988 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 03:46:31,995 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 03:46:31,996 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 03:46:32,000 INFO ___FILE_ONLY___ (unset)
5
+
6
+ 2025-11-08 03:46:32,000 DEBUG root Chosen display Format:value(.)
7
+ 2025-11-08 03:46:32,000 INFO root Display format: "value(.)"
8
+ 2025-11-08 03:46:32,026 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.46.32.585013.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:46:32,587 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 03:46:32,590 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 03:46:32,592 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 03:46:32,597 INFO ___FILE_ONLY___ (unset)
5
+
6
+ 2025-11-08 03:46:32,597 DEBUG root Chosen display Format:value(.)
7
+ 2025-11-08 03:46:32,598 INFO root Display format: "value(.)"
8
+ 2025-11-08 03:46:32,624 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.56.10.930875.log ADDED
@@ -0,0 +1,15 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:56:10,932 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 03:56:11,103 DEBUG root Loaded Command Group: ['gcloud', 'config', 'set']
3
+ 2025-11-08 03:56:11,106 DEBUG root Running [gcloud.config.set] with arguments: [SECTION/PROPERTY: "project", VALUE: "coherent-ascent-464321-s9"]
4
+ 2025-11-08 03:56:11,113 WARNING root Your active project does not match the quota project in your local Application Default Credentials file. This might result in unexpected quota issues.
5
+
6
+ To update your Application Default Credentials quota project, use the `gcloud auth application-default set-quota-project` command.
7
+ 2025-11-08 03:56:11,172 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): cloudresourcemanager.googleapis.com:443
8
+ 2025-11-08 03:56:12,234 DEBUG urllib3.connectionpool https://cloudresourcemanager.googleapis.com:443 "GET /v1/projects/coherent-ascent-464321-s9?alt=json HTTP/1.1" 200 None
9
+ 2025-11-08 03:56:12,235 INFO ___FILE_ONLY___ INFORMATION: Project 'coherent-ascent-464321-s9' has no 'environment' tag set. Use either 'Production', 'Development', 'Test', or 'Staging'. Add an 'environment' tag using `gcloud resource-manager tags bindings create`.
10
+
11
+ 2025-11-08 03:56:12,236 INFO ___FILE_ONLY___ Updated property [core/project].
12
+
13
+ 2025-11-08 03:56:12,237 DEBUG root Chosen display Format:default
14
+ 2025-11-08 03:56:12,238 INFO root Display format: "default"
15
+ 2025-11-08 03:56:12,243 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.56.28.454394.log ADDED
@@ -0,0 +1,22 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:56:28,455 DEBUG root Loaded Command Group: ['gcloud', 'auth']
2
+ 2025-11-08 03:56:28,458 DEBUG root Loaded Command Group: ['gcloud', 'auth', 'application_default']
3
+ 2025-11-08 03:56:28,484 DEBUG root Loaded Command Group: ['gcloud', 'auth', 'application-default', 'set_quota_project']
4
+ 2025-11-08 03:56:28,485 DEBUG root Running [gcloud.auth.application-default.set-quota-project] with arguments: [QUOTA_PROJECT_ID: "coherent-ascent-464321-s9"]
5
+ 2025-11-08 03:56:28,504 INFO root Using alternate credentials from file: [/content/.config/application_default_credentials.json]
6
+ 2025-11-08 03:56:28,510 DEBUG google.auth.transport.requests Making request: POST https://oauth2.googleapis.com/token
7
+ 2025-11-08 03:56:28,511 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): oauth2.googleapis.com:443
8
+ 2025-11-08 03:56:28,551 DEBUG urllib3.connectionpool https://oauth2.googleapis.com:443 "POST /token HTTP/1.1" 200 None
9
+ 2025-11-08 03:56:28,566 DEBUG urllib3.connectionpool Starting new HTTPS connection (1): cloudresourcemanager.googleapis.com:443
10
+ 2025-11-08 03:56:29,574 DEBUG urllib3.connectionpool https://cloudresourcemanager.googleapis.com:443 "POST /v1/projects/coherent-ascent-464321-s9:testIamPermissions?alt=json HTTP/1.1" 200 None
11
+ 2025-11-08 03:56:29,575 INFO ___FILE_ONLY___
12
+ Credentials saved to file: [/content/.config/application_default_credentials.json]
13
+
14
+ 2025-11-08 03:56:29,576 INFO ___FILE_ONLY___
15
+ These credentials will be used by any library that requests Application Default Credentials (ADC).
16
+
17
+ 2025-11-08 03:56:29,576 INFO ___FILE_ONLY___
18
+ Quota project "coherent-ascent-464321-s9" was added to ADC which can be used by Google client libraries for billing and quota. Note that some services may still bill the project owning the resource.
19
+
20
+ 2025-11-08 03:56:29,576 DEBUG root Chosen display Format:none
21
+ 2025-11-08 03:56:29,576 INFO root Display format: "none"
22
+ 2025-11-08 03:56:29,602 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.56.56.000749.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:56:56,002 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 03:56:56,006 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 03:56:56,008 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 03:56:56,008 DEBUG root Chosen display Format:value(.)
5
+ 2025-11-08 03:56:56,009 INFO root Display format: "value(.)"
6
+ 2025-11-08 03:56:56,009 INFO ___FILE_ONLY___ coherent-ascent-464321-s9
7
+
8
+ 2025-11-08 03:56:56,049 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/03.56.56.815151.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 03:56:56,817 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 03:56:56,820 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 03:56:56,822 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 03:56:56,822 DEBUG root Chosen display Format:value(.)
5
+ 2025-11-08 03:56:56,822 INFO root Display format: "value(.)"
6
+ 2025-11-08 03:56:56,823 INFO ___FILE_ONLY___ coherent-ascent-464321-s9
7
+
8
+ 2025-11-08 03:56:56,857 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/04.00.48.739317.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 04:00:48,742 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 04:00:48,752 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 04:00:48,753 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 04:00:48,754 DEBUG root Chosen display Format:value(.)
5
+ 2025-11-08 04:00:48,754 INFO root Display format: "value(.)"
6
+ 2025-11-08 04:00:48,754 INFO ___FILE_ONLY___ coherent-ascent-464321-s9
7
+
8
+ 2025-11-08 04:00:48,814 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/04.00.49.407053.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 04:00:49,408 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 04:00:49,410 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 04:00:49,411 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 04:00:49,411 DEBUG root Chosen display Format:value(.)
5
+ 2025-11-08 04:00:49,412 INFO root Display format: "value(.)"
6
+ 2025-11-08 04:00:49,412 INFO ___FILE_ONLY___ coherent-ascent-464321-s9
7
+
8
+ 2025-11-08 04:00:49,437 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/04.02.17.927382.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 04:02:17,930 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 04:02:17,937 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 04:02:17,939 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 04:02:17,940 DEBUG root Chosen display Format:value(.)
5
+ 2025-11-08 04:02:17,940 INFO root Display format: "value(.)"
6
+ 2025-11-08 04:02:17,941 INFO ___FILE_ONLY___ coherent-ascent-464321-s9
7
+
8
+ 2025-11-08 04:02:18,022 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/04.02.19.083433.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 04:02:19,085 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 04:02:19,089 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 04:02:19,091 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 04:02:19,091 DEBUG root Chosen display Format:value(.)
5
+ 2025-11-08 04:02:19,092 INFO root Display format: "value(.)"
6
+ 2025-11-08 04:02:19,092 INFO ___FILE_ONLY___ coherent-ascent-464321-s9
7
+
8
+ 2025-11-08 04:02:19,137 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/04.04.13.667264.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 04:04:13,669 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 04:04:13,676 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 04:04:13,678 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 04:04:13,680 DEBUG root Chosen display Format:value(.)
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+ 2025-11-08 04:04:13,681 INFO root Display format: "value(.)"
6
+ 2025-11-08 04:04:13,681 INFO ___FILE_ONLY___ coherent-ascent-464321-s9
7
+
8
+ 2025-11-08 04:04:13,768 DEBUG root SDK update checks are disabled.
.config/logs/2025.11.08/04.04.14.707530.log ADDED
@@ -0,0 +1,8 @@
 
 
 
 
 
 
 
 
 
1
+ 2025-11-08 04:04:14,710 DEBUG root Loaded Command Group: ['gcloud', 'config']
2
+ 2025-11-08 04:04:14,713 DEBUG root Loaded Command Group: ['gcloud', 'config', 'get']
3
+ 2025-11-08 04:04:14,715 DEBUG root Running [gcloud.config.get] with arguments: [SECTION/PROPERTY: "project"]
4
+ 2025-11-08 04:04:14,716 DEBUG root Chosen display Format:value(.)
5
+ 2025-11-08 04:04:14,716 INFO root Display format: "value(.)"
6
+ 2025-11-08 04:04:14,716 INFO ___FILE_ONLY___ coherent-ascent-464321-s9
7
+
8
+ 2025-11-08 04:04:14,753 DEBUG root SDK update checks are disabled.
.gitattributes CHANGED
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  *.zip filter=lfs diff=lfs merge=lfs -text
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  *.zst filter=lfs diff=lfs merge=lfs -text
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  *tfevents* filter=lfs diff=lfs merge=lfs -text
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+ hplc_lora/tokenizer.json filter=lfs diff=lfs merge=lfs -text
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+ outputs_hplc/checkpoint-1/tokenizer.json filter=lfs diff=lfs merge=lfs -text
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+ outputs_hplc/checkpoint-3/tokenizer.json filter=lfs diff=lfs merge=lfs -text
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+ sample_data/mnist_test.csv filter=lfs diff=lfs merge=lfs -text
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+ sample_data/mnist_train_small.csv filter=lfs diff=lfs merge=lfs -text
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+ unsloth_compiled_cache/__pycache__/UnslothGRPOTrainer.cpython-312.pyc filter=lfs diff=lfs merge=lfs -text
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+ unsloth_compiled_cache/__pycache__/UnslothOnlineDPOTrainer.cpython-312.pyc filter=lfs diff=lfs merge=lfs -text
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+ unsloth_compiled_cache/__pycache__/UnslothRLOOTrainer.cpython-312.pyc filter=lfs diff=lfs merge=lfs -text
.gradio/certificate.pem ADDED
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+ -----END CERTIFICATE-----
README.md CHANGED
@@ -1,12 +1,6 @@
1
  ---
2
- title: Llama-3 HPLC Method Development Troubleshooting
3
- emoji: 🌍
4
- colorFrom: blue
5
- colorTo: gray
6
  sdk: gradio
7
  sdk_version: 5.49.1
8
- app_file: app.py
9
- pinned: false
10
  ---
11
-
12
- Check out the configuration reference at https://huggingface.co/docs/hub/spaces-config-reference
 
1
  ---
2
+ title: Llama-3_HPLC_Method_Development__Troubleshooting
3
+ app_file: app.py
 
 
4
  sdk: gradio
5
  sdk_version: 5.49.1
 
 
6
  ---
 
 
app.py ADDED
@@ -0,0 +1,67 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ import gradio as gr
2
+ import torch
3
+ import unsloth
4
+ from unsloth import FastLanguageModel
5
+ from transformers import TextStreamer
6
+
7
+ # Define model and tokenizer loading parameters
8
+ MODEL_NAME = "unsloth/llama-3-8b-Instruct-bnb-4bit"
9
+ max_seq_length = 2048
10
+ load_in_4bit = True
11
+ dtype = None
12
+
13
+ # Load the base model and then the fine-tuned LoRA adapter
14
+ model, tokenizer = FastLanguageModel.from_pretrained(
15
+ model_name=MODEL_NAME,
16
+ max_seq_length=max_seq_length,
17
+ dtype=dtype,
18
+ load_in_4bit=load_in_4bit,
19
+ )
20
+
21
+ # Load the LoRA adapter from the saved directory
22
+ # This assumes 'hplc_lora' is in the current working directory
23
+ # or in the directory where the app will be deployed.
24
+ model.load_adapter("hplc_lora")
25
+
26
+ # Ensure tokenizer pad_token is set for generation
27
+ tokenizer.pad_token = tokenizer.eos_token
28
+ tokenizer.padding_side = "right"
29
+
30
+ # --- Simple inference function using your fine-tuned model ---
31
+ def hplc_chat(user_prompt):
32
+ system_prompt = (
33
+ "You are an expert analytical chemist specializing in HPLC method development "
34
+ "and troubleshooting. Give concise, step-by-step actions with reasoning, "
35
+ "numeric targets, and acceptance criteria."
36
+ )
37
+ # Combine into chat-style prompt string
38
+ text = f"<|begin_of_text|><|start_header_id|>system<|end_header_id|>\n{system_prompt}\n\n" \
39
+ f"<|start_header_id|>user<|end_header_id|>\n{user_prompt}\n\n" \
40
+ f"<|start_header_id|>assistant<|end_header_id|>\n"
41
+ inputs = tokenizer([text], return_tensors="pt").to(model.device)
42
+
43
+ with torch.no_grad():
44
+ output = model.generate(
45
+ **inputs,
46
+ max_new_tokens=400,
47
+ temperature=0.4,
48
+ top_p=0.9,
49
+ eos_token_id=tokenizer.eos_token_id, # Use EOS token to stop generation
50
+ pad_token_id=tokenizer.pad_token_id # Use pad_token_id during generation
51
+ )
52
+ answer = tokenizer.decode(output[0], skip_special_tokens=True)
53
+ # Extract only the assistant's response part
54
+ return answer.split("assistant")[-1].strip()
55
+
56
+ # --- Build the Gradio interface ---
57
+ demo = gr.Interface(
58
+ fn=hplc_chat,
59
+ inputs=gr.Textbox(label="Enter your HPLC question or problem", lines=5, placeholder="e.g., Retention time drift >5%"),
60
+ outputs=gr.Textbox(label="Model response", lines=12),
61
+ title="🧪 Llama-3 HPLC Method Development & Troubleshooting",
62
+ description="Ask about gradient setup, peak shape, pressure, or column issues. The model will suggest step-by-step fixes and acceptance criteria.",
63
+ )
64
+
65
+ # Launch the Gradio app if the script is run directly
66
+ if __name__ == "__main__":
67
+ demo.launch(share=True)
data/hplc.jsonl ADDED
@@ -0,0 +1,100 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ {"instruction": "Fix peak tailing/broadening/splitting.", "input": "Matrix: river water; Analytes: azo dyes; Column: C18, 2.1×100 mm, 1.7 µm; Oven 25°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Gradient 10→95% B over 6 min; Flow 0.30 mL/min. Observation: tailing factor ~1.8; occasional split peaks on main analyte.", "output": "Likely causes and actions:\n1) Match sample solvent to initial mobile phase; dilute strong solvents.\n2) Lower pH to suppress ionization for bases (pH 2.8–3.2) or raise for acids (pH 6.5–7.5).\n3) Consider polar-embedded/C18-AQ or PFP to reduce secondary interactions.\n4) Replace guard; check for column void (reverse flush briefly).\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "peak_shape", "detector": "FLD", "instrument": "HPLC"}}
2
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: QC standard in water; Analytes: azo dyes; Column: C18, 2.1×100 mm, 1.7 µm; Oven 45°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Isocratic 50% B; Flow 0.50 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "carryover", "detector": "MS", "instrument": "UHPLC"}}
3
+ {"instruction": "Diagnose column frit clogging or void formation.", "input": "Matrix: serum; Analytes: sugars; Column: C8, 4.6×100 mm, 3.5 µm; Oven 35°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 10→80% B over 6 min; Flow 0.50 mL/min. Observation: plate count dropped 40%; asymmetry 1.7; possible void.", "output": "Likely causes and actions:\n1) Check asymmetry and plates with a system suitability compound.\n2) Reverse-flush 5–10 min; replace guard; inspect for voids (shoulder/doublet).\n3) Reduce max pressure; verify fittings/ferrules to prevent channeling.\n4) Log column volumes; retire if performance out of spec.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "column_health", "detector": "UV", "instrument": "HPLC"}}
4
+ {"instruction": "Diagnose bubble/degassing issues causing ripple.", "input": "Matrix: wastewater effluent; Analytes: acidic herbicides (pKa 2–4); Column: PFP, 2.1×100 mm, 1.9 µm; Oven 30°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Isocratic 50% B; Flow 0.50 mL/min. Observation: periodic bubble bursts; UV shows spikes.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "degassing", "detector": "FLD", "instrument": "HPLC"}}
5
+ {"instruction": "Diagnose bubble/degassing issues causing ripple.", "input": "Matrix: wine; Analytes: quinolones; Column: Phenyl-hexyl, 3.0×100 mm, 3 µm; Oven 35°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Gradient 10→60% B over 15 min; Flow 0.30 mL/min. Observation: periodic bubble bursts; UV shows spikes.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "degassing", "detector": "FLD", "instrument": "UHPLC"}}
6
+ {"instruction": "Fix peak tailing/broadening/splitting.", "input": "Matrix: urine; Analytes: azo dyes; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 25°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 35% B; Flow 0.50 mL/min. Observation: tailing factor ~1.8; occasional split peaks on main analyte.", "output": "Likely causes and actions:\n1) Match sample solvent to initial mobile phase; dilute strong solvents.\n2) Lower pH to suppress ionization for bases (pH 2.8–3.2) or raise for acids (pH 6.5–7.5).\n3) Consider polar-embedded/C18-AQ or PFP to reduce secondary interactions.\n4) Replace guard; check for column void (reverse flush briefly).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "peak_shape", "detector": "FLD", "instrument": "HPLC"}}
7
+ {"instruction": "Fix peak tailing/broadening/splitting.", "input": "Matrix: tablet dissolution (pH 6.8); Analytes: phenols; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 30°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Isocratic 50% B; Flow 0.50 mL/min. Observation: tailing factor ~1.8; occasional split peaks on main analyte.", "output": "Likely causes and actions:\n1) Match sample solvent to initial mobile phase; dilute strong solvents.\n2) Lower pH to suppress ionization for bases (pH 2.8–3.2) or raise for acids (pH 6.5–7.5).\n3) Consider polar-embedded/C18-AQ or PFP to reduce secondary interactions.\n4) Replace guard; check for column void (reverse flush briefly).\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "peak_shape", "detector": "PDA", "instrument": "HPLC"}}
8
+ {"instruction": "Resolve pump pulsation/check valve problems.", "input": "Matrix: QC standard in water; Analytes: steroids; Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 30°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Isocratic 50% B; Flow 1.0 mL/min. Observation: pulsation visible on pressure trace; peak area RSD ~6%.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "pump", "detector": "UV", "instrument": "HPLC"}}
9
+ {"instruction": "Resolve pump pulsation/check valve problems.", "input": "Matrix: cosmetics extract (IPA); Analytes: alkylbenzene sulfonates; Column: C18, 2.1×100 mm, 1.7 µm; Oven 35°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 2→95% B over 6 min; Flow 1.0 mL/min. Observation: pulsation visible on pressure trace; peak area RSD ~6%.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "pump", "detector": "UV", "instrument": "UHPLC"}}
10
+ {"instruction": "Diagnose column frit clogging or void formation.", "input": "Matrix: milk; Analytes: beta-lactams; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 45°C; A: water + 0.05% TFA; B: ACN; Gradient 10→80% B over 12 min; Flow 0.30 mL/min. Observation: plate count dropped 40%; asymmetry 1.7; possible void.", "output": "Likely causes and actions:\n1) Check asymmetry and plates with a system suitability compound.\n2) Reverse-flush 5–10 min; replace guard; inspect for voids (shoulder/doublet).\n3) Reduce max pressure; verify fittings/ferrules to prevent channeling.\n4) Log column volumes; retire if performance out of spec.\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "column_health", "detector": "MS", "instrument": "UHPLC"}}
11
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: milk; Analytes: acidic herbicides (pKa 2–4); Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 40°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 10→60% B over 8 min; Flow 0.30 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "carryover", "detector": "UV", "instrument": "HPLC"}}
12
+ {"instruction": "Diagnose retention time drift >5%.", "input": "Matrix: oil matrix (hexane); Analytes: polyphenols; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 35°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Gradient 10→95% B over 6 min; Flow 1.0 mL/min. Observation: RT drifts +6–10% over a batch of 30 samples.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "RT_drift", "detector": "MS", "instrument": "HPLC"}}
13
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: drinking water; Analytes: acidic herbicides (pKa 2–4); Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 25°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Gradient 2→40% B over 10 min; Flow 0.40 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "carryover", "detector": "MS", "instrument": "HPLC"}}
14
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: tablet dissolution (pH 6.8); Analytes: azo dyes; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 40°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 50% B; Flow 1.0 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "baseline", "detector": "MS", "instrument": "UHPLC"}}
15
+ {"instruction": "Troubleshoot high backpressure increase vs baseline.", "input": "Matrix: pharma API in diluent; Analytes: sugars; Column: C18, 2.1×100 mm, 1.7 µm; Oven 35°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Isocratic 60% B; Flow 0.30 mL/min. Observation: pressure increased 30–50% vs yesterday; pulses under gradient.", "output": "Likely causes and actions:\n1) Bypass column to assess system backpressure; replace inline filter and frits (0.2 µm).\n2) Flush column: 50:50 water:organic (10 min) → 100% organic (10 min) → 100% water (10 min).\n3) Check solvent miscibility; remove salts before 100% organic to avoid precipitation.\n4) Inspect pump seals/check valves; verify degasser performance (vacuum level OK).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "pressure", "detector": "UV", "instrument": "HPLC"}}
16
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: soil extract (MeOH); Analytes: acidic herbicides (pKa 2–4); Column: PFP, 2.1×100 mm, 1.9 µm; Oven 45°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Gradient 2→40% B over 6 min; Flow 0.30 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "baseline", "detector": "PDA", "instrument": "HPLC"}}
17
+ {"instruction": "Troubleshoot high backpressure increase vs baseline.", "input": "Matrix: tablet dissolution (pH 6.8); Analytes: beta-lactams; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 25°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Isocratic 50% B; Flow 1.0 mL/min. Observation: pressure increased 30–50% vs yesterday; pulses under gradient.", "output": "Likely causes and actions:\n1) Bypass column to assess system backpressure; replace inline filter and frits (0.2 µm).\n2) Flush column: 50:50 water:organic (10 min) → 100% organic (10 min) → 100% water (10 min).\n3) Check solvent miscibility; remove salts before 100% organic to avoid precipitation.\n4) Inspect pump seals/check valves; verify degasser performance (vacuum level OK).\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "pressure", "detector": "PDA", "instrument": "HPLC"}}
18
+ {"instruction": "Ensure sufficient column equilibration between runs.", "input": "Matrix: milk; Analytes: alkylbenzene sulfonates; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 25°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 10→60% B over 6 min; Flow 1.0 mL/min. Observation: first run after gradient shift differs from subsequent runs.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "equilibration", "detector": "UV", "instrument": "HPLC"}}
19
+ {"instruction": "Diagnose column frit clogging or void formation.", "input": "Matrix: wastewater effluent; Analytes: steroids; Column: C18, 4.6×150 mm, 5 µm; Oven 30°C; A: water + 0.05% TFA; B: ACN; Isocratic 60% B; Flow 0.30 mL/min. Observation: plate count dropped 40%; asymmetry 1.7; possible void.", "output": "Likely causes and actions:\n1) Check asymmetry and plates with a system suitability compound.\n2) Reverse-flush 5–10 min; replace guard; inspect for voids (shoulder/doublet).\n3) Reduce max pressure; verify fittings/ferrules to prevent channeling.\n4) Log column volumes; retire if performance out of spec.\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "column_health", "detector": "MS", "instrument": "UHPLC"}}
20
+ {"instruction": "Diagnose retention time drift >5%.", "input": "Matrix: serum; Analytes: sugars; Column: C8, 4.6×100 mm, 3.5 µm; Oven 35°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Gradient 5→40% B over 6 min; Flow 1.0 mL/min. Observation: RT drifts +6–10% over a batch of 30 samples.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "RT_drift", "detector": "MS", "instrument": "UHPLC"}}
21
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: serum; Analytes: acidic herbicides (pKa 2–4); Column: PFP, 2.1×100 mm, 1.9 µm; Oven 35°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Gradient 2→95% B over 15 min; Flow 0.50 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "temperature", "detector": "MS", "instrument": "UHPLC"}}
22
+ {"instruction": "Fix peak tailing/broadening/splitting.", "input": "Matrix: milk; Analytes: steroids; Column: C18, 4.6×150 mm, 5 µm; Oven 45°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Isocratic 35% B; Flow 0.40 mL/min. Observation: tailing factor ~1.8; occasional split peaks on main analyte.", "output": "Likely causes and actions:\n1) Match sample solvent to initial mobile phase; dilute strong solvents.\n2) Lower pH to suppress ionization for bases (pH 2.8–3.2) or raise for acids (pH 6.5–7.5).\n3) Consider polar-embedded/C18-AQ or PFP to reduce secondary interactions.\n4) Replace guard; check for column void (reverse flush briefly).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "peak_shape", "detector": "MS", "instrument": "UHPLC"}}
23
+ {"instruction": "Troubleshoot high backpressure increase vs baseline.", "input": "Matrix: urine; Analytes: acidic herbicides (pKa 2–4); Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 40°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Isocratic 35% B; Flow 0.50 mL/min. Observation: pressure increased 30–50% vs yesterday; pulses under gradient.", "output": "Likely causes and actions:\n1) Bypass column to assess system backpressure; replace inline filter and frits (0.2 µm).\n2) Flush column: 50:50 water:organic (10 min) → 100% organic (10 min) → 100% water (10 min).\n3) Check solvent miscibility; remove salts before 100% organic to avoid precipitation.\n4) Inspect pump seals/check valves; verify degasser performance (vacuum level OK).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "pressure", "detector": "FLD", "instrument": "HPLC"}}
24
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: pharma API in diluent; Analytes: acidic herbicides (pKa 2–4); Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 30°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Gradient 5→60% B over 12 min; Flow 0.30 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "carryover", "detector": "UV", "instrument": "HPLC"}}
25
+ {"instruction": "Diagnose column frit clogging or void formation.", "input": "Matrix: wastewater influent; Analytes: steroids; Column: C8, 4.6×100 mm, 3.5 µm; Oven 45°C; A: water + 0.1% FA; B: ACN; Gradient 10→60% B over 8 min; Flow 1.0 mL/min. Observation: plate count dropped 40%; asymmetry 1.7; possible void.", "output": "Likely causes and actions:\n1) Check asymmetry and plates with a system suitability compound.\n2) Reverse-flush 5–10 min; replace guard; inspect for voids (shoulder/doublet).\n3) Reduce max pressure; verify fittings/ferrules to prevent channeling.\n4) Log column volumes; retire if performance out of spec.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "column_health", "detector": "MS", "instrument": "HPLC"}}
26
+ {"instruction": "Ensure sufficient column equilibration between runs.", "input": "Matrix: drinking water; Analytes: azo dyes; Column: Phenyl-hexyl, 3.0×100 mm, 3 µm; Oven 30°C; A: water + 0.1% FA; B: ACN; Gradient 5→40% B over 12 min; Flow 0.30 mL/min. Observation: first run after gradient shift differs from subsequent runs.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "equilibration", "detector": "FLD", "instrument": "UHPLC"}}
27
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: wine; Analytes: basic amines (pKa 8–10); Column: PFP, 2.1×100 mm, 1.9 µm; Oven 30°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Isocratic 40% B; Flow 1.0 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "temperature", "detector": "MS", "instrument": "HPLC"}}
28
+ {"instruction": "Resolve buffer pH/precipitation/mixing problems.", "input": "Matrix: leaf extract; Analytes: phenols; Column: C8, 4.6×100 mm, 3.5 µm; Oven 45°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Isocratic 50% B; Flow 0.50 mL/min. Observation: salt precipitation suspected at high % organic.", "output": "Likely causes and actions:\n1) Use volatile buffers (ammonium formate/acetate) at ≤10–20 mM for MS; check pH at run temp.\n2) Avoid high-salt transitions to 100% organic; flush with salt-free aqueous before.\n3) Filter and degas buffers; replace after 48 h; verify miscibility A/B.\n4) At low pH for bases, add 0.1% FA; for strong tailing, consider 2–5 mM TFA (UV penalty).\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "buffer", "detector": "UV", "instrument": "HPLC"}}
29
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: food homogenate; Analytes: phenols; Column: C18, 4.6×150 mm, 5 µm; Oven 40°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 10→60% B over 10 min; Flow 0.30 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "temperature", "detector": "PDA", "instrument": "HPLC"}}
30
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: oil matrix (hexane); Analytes: vitamins (B-group); Column: C8, 4.6×100 mm, 3.5 µm; Oven 40°C; A: water + 0.1% FA; B: ACN; Gradient 5→80% B over 15 min; Flow 0.50 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "baseline", "detector": "UV", "instrument": "UHPLC"}}
31
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: leaf extract; Analytes: basic amines (pKa 8–10); Column: Phenyl-hexyl, 3.0×100 mm, 3 µm; Oven 35°C; A: water + 0.1% FA; B: ACN; Isocratic 50% B; Flow 0.40 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "temperature", "detector": "FLD", "instrument": "UHPLC"}}
32
+ {"instruction": "Decide on ion-pairing conditions and caveats.", "input": "Matrix: QC standard in water; Analytes: polyphenols; Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 45°C; A: water + 0.05% TFA; B: ACN; Gradient 2→60% B over 20 min; Flow 0.50 mL/min. Observation: basic amines tail at pH>6; poor retention at low organic.", "output": "Likely causes and actions:\n1) Try without ion-pair first using acidic pH and endcapped C18; assess tailing.\n2) If needed, test HFBA/TFA (UV penalty) or volatile IP reagents for MS with caution.\n3) Document equilibration burden and memory effects; dedicate system if adopting IP.\n4) Consider polar-embedded or mixed-mode columns as alternatives.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "ion_pair", "detector": "MS", "instrument": "HPLC"}}
33
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: groundwater; Analytes: caffeine analogs; Column: C18, 2.1×100 mm, 1.7 µm; Oven 25°C; A: water + 0.05% TFA; B: ACN; Isocratic 50% B; Flow 1.0 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "baseline", "detector": "UV", "instrument": "HPLC"}}
34
+ {"instruction": "Decide on ion-pairing conditions and caveats.", "input": "Matrix: groundwater; Analytes: azo dyes; Column: C18, 2.1×100 mm, 1.7 µm; Oven 45°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Gradient 2→60% B over 6 min; Flow 1.0 mL/min. Observation: basic amines tail at pH>6; poor retention at low organic.", "output": "Likely causes and actions:\n1) Try without ion-pair first using acidic pH and endcapped C18; assess tailing.\n2) If needed, test HFBA/TFA (UV penalty) or volatile IP reagents for MS with caution.\n3) Document equilibration burden and memory effects; dedicate system if adopting IP.\n4) Consider polar-embedded or mixed-mode columns as alternatives.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "ion_pair", "detector": "UV", "instrument": "UHPLC"}}
35
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: QC standard in water; Analytes: glycosides; Column: C8, 4.6×100 mm, 3.5 µm; Oven 40°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Isocratic 40% B; Flow 1.0 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "temperature", "detector": "PDA", "instrument": "UHPLC"}}
36
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: urine; Analytes: azo dyes; Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 35°C; A: water + 0.1% FA; B: ACN; Gradient 10→40% B over 8 min; Flow 0.50 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "baseline", "detector": "UV", "instrument": "UHPLC"}}
37
+ {"instruction": "Diagnose column frit clogging or void formation.", "input": "Matrix: groundwater; Analytes: bile acids; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 45°C; A: water + 0.1% FA; B: ACN; Isocratic 50% B; Flow 1.0 mL/min. Observation: plate count dropped 40%; asymmetry 1.7; possible void.", "output": "Likely causes and actions:\n1) Check asymmetry and plates with a system suitability compound.\n2) Reverse-flush 5–10 min; replace guard; inspect for voids (shoulder/doublet).\n3) Reduce max pressure; verify fittings/ferrules to prevent channeling.\n4) Log column volumes; retire if performance out of spec.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "column_health", "detector": "UV", "instrument": "UHPLC"}}
38
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: drinking water; Analytes: amino acids; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 40°C; A: water + 0.1% FA; B: ACN; Isocratic 35% B; Flow 1.0 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "baseline", "detector": "PDA", "instrument": "HPLC"}}
39
+ {"instruction": "Diagnose retention time drift >5%.", "input": "Matrix: drinking water; Analytes: beta-lactams; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 40°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Gradient 10→40% B over 12 min; Flow 0.40 mL/min. Observation: RT drifts +6–10% over a batch of 30 samples.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "RT_drift", "detector": "FLD", "instrument": "UHPLC"}}
40
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: food homogenate; Analytes: neutral pesticides; Column: Phenyl-hexyl, 3.0×100 mm, 3 µm; Oven 40°C; A: water + 0.1% FA; B: ACN; Isocratic 40% B; Flow 0.50 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "carryover", "detector": "PDA", "instrument": "HPLC"}}
41
+ {"instruction": "Ensure sufficient column equilibration between runs.", "input": "Matrix: urine; Analytes: neutral pesticides; Column: C18, 4.6×150 mm, 5 µm; Oven 30°C; A: water + 0.1% FA; B: ACN; Gradient 10→40% B over 6 min; Flow 0.30 mL/min. Observation: first run after gradient shift differs from subsequent runs.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "equilibration", "detector": "PDA", "instrument": "HPLC"}}
42
+ {"instruction": "Decide on ion-pairing conditions and caveats.", "input": "Matrix: oil matrix (hexane); Analytes: caffeine analogs; Column: Phenyl-hexyl, 3.0×100 mm, 3 µm; Oven 25°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Gradient 5→80% B over 10 min; Flow 0.40 mL/min. Observation: basic amines tail at pH>6; poor retention at low organic.", "output": "Likely causes and actions:\n1) Try without ion-pair first using acidic pH and endcapped C18; assess tailing.\n2) If needed, test HFBA/TFA (UV penalty) or volatile IP reagents for MS with caution.\n3) Document equilibration burden and memory effects; dedicate system if adopting IP.\n4) Consider polar-embedded or mixed-mode columns as alternatives.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "ion_pair", "detector": "MS", "instrument": "HPLC"}}
43
+ {"instruction": "Decide on ion-pairing conditions and caveats.", "input": "Matrix: milk; Analytes: sugars; Column: C18, 4.6×150 mm, 5 µm; Oven 30°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Gradient 10→60% B over 6 min; Flow 0.30 mL/min. Observation: basic amines tail at pH>6; poor retention at low organic.", "output": "Likely causes and actions:\n1) Try without ion-pair first using acidic pH and endcapped C18; assess tailing.\n2) If needed, test HFBA/TFA (UV penalty) or volatile IP reagents for MS with caution.\n3) Document equilibration burden and memory effects; dedicate system if adopting IP.\n4) Consider polar-embedded or mixed-mode columns as alternatives.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "ion_pair", "detector": "UV", "instrument": "UHPLC"}}
44
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: wastewater influent; Analytes: acidic herbicides (pKa 2–4); Column: C8, 4.6×100 mm, 3.5 µm; Oven 35°C; A: water + 0.05% TFA; B: ACN; Isocratic 50% B; Flow 0.30 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "temperature", "detector": "PDA", "instrument": "UHPLC"}}
45
+ {"instruction": "Fix peak tailing/broadening/splitting.", "input": "Matrix: groundwater; Analytes: caffeine analogs; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 40°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Isocratic 40% B; Flow 0.50 mL/min. Observation: tailing factor ~1.8; occasional split peaks on main analyte.", "output": "Likely causes and actions:\n1) Match sample solvent to initial mobile phase; dilute strong solvents.\n2) Lower pH to suppress ionization for bases (pH 2.8–3.2) or raise for acids (pH 6.5–7.5).\n3) Consider polar-embedded/C18-AQ or PFP to reduce secondary interactions.\n4) Replace guard; check for column void (reverse flush briefly).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "peak_shape", "detector": "MS", "instrument": "UHPLC"}}
46
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: polymer extract; Analytes: caffeine analogs; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 35°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 40% B; Flow 0.40 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "carryover", "detector": "FLD", "instrument": "HPLC"}}
47
+ {"instruction": "Diagnose bubble/degassing issues causing ripple.", "input": "Matrix: urine; Analytes: azo dyes; Column: C18, 2.1×100 mm, 1.7 µm; Oven 40°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Isocratic 50% B; Flow 1.0 mL/min. Observation: periodic bubble bursts; UV shows spikes.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "degassing", "detector": "MS", "instrument": "HPLC"}}
48
+ {"instruction": "Eliminate ghost peaks seen in solvent blanks.", "input": "Matrix: wastewater effluent; Analytes: azo dyes; Column: PFP, 2.1×100 mm, 1.9 µm; Oven 45°C; A: water + 0.1% FA; B: ACN; Isocratic 60% B; Flow 0.50 mL/min. Observation: ghost peaks in solvent blanks at random RTs.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "ghost_peaks", "detector": "MS", "instrument": "UHPLC"}}
49
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: pharma API in diluent; Analytes: azo dyes; Column: PFP, 2.1×100 mm, 1.9 µm; Oven 30°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Isocratic 35% B; Flow 0.50 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "carryover", "detector": "FLD", "instrument": "UHPLC"}}
50
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: polymer extract; Analytes: polyphenols; Column: PFP, 2.1×100 mm, 1.9 µm; Oven 35°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Gradient 5→40% B over 20 min; Flow 0.40 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "carryover", "detector": "UV", "instrument": "HPLC"}}
51
+ {"instruction": "Propose starting conditions for HPLC method development.", "input": "Matrix: wastewater influent; Analytes: steroids; Column: PFP, 2.1×100 mm, 1.9 µm; Oven 25°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Gradient 5→95% B over 12 min; Flow 0.50 mL/min. Goal: fast baseline method to separate target class within 8–12 min.", "output": "Likely causes and actions:\n1) Start: C18 or PFP; 35–40°C; 1.0 mL/min (4.6 mm) or 0.3–0.5 mL/min (2.1 mm).\n2) Acidic mobile for bases: A 10 mM ammonium formate + 0.1% FA; B ACN.\n3) Gradient 5→40% B in 10–12 min; adjust to hit k′ 2–10 and Rs>1.5 for critical pairs.\n4) If poor retention at low %B, try C18-AQ or phenyl-hexyl; consider HILIC for very polar.\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "method_dev", "detector": "MS", "instrument": "UHPLC"}}
52
+ {"instruction": "Fix peak tailing/broadening/splitting.", "input": "Matrix: tablet dissolution (pH 6.8); Analytes: caffeine analogs; Column: C18, 4.6×150 mm, 5 µm; Oven 45°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 2→60% B over 15 min; Flow 0.30 mL/min. Observation: tailing factor ~1.8; occasional split peaks on main analyte.", "output": "Likely causes and actions:\n1) Match sample solvent to initial mobile phase; dilute strong solvents.\n2) Lower pH to suppress ionization for bases (pH 2.8–3.2) or raise for acids (pH 6.5–7.5).\n3) Consider polar-embedded/C18-AQ or PFP to reduce secondary interactions.\n4) Replace guard; check for column void (reverse flush briefly).\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "peak_shape", "detector": "UV", "instrument": "UHPLC"}}
53
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: cosmetics extract (IPA); Analytes: glycosides; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 35°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Gradient 5→40% B over 15 min; Flow 1.0 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "temperature", "detector": "MS", "instrument": "UHPLC"}}
54
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: urine; Analytes: beta-lactams; Column: C18, 4.6×150 mm, 5 µm; Oven 25°C; A: water + 0.05% TFA; B: ACN; Isocratic 60% B; Flow 0.40 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "carryover", "detector": "MS", "instrument": "HPLC"}}
55
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: plasma; Analytes: acidic herbicides (pKa 2–4); Column: PFP, 2.1×100 mm, 1.9 µm; Oven 30°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Gradient 10→60% B over 12 min; Flow 0.50 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "temperature", "detector": "FLD", "instrument": "UHPLC"}}
56
+ {"instruction": "Reduce carryover observed in blanks after high standards.", "input": "Matrix: urine; Analytes: beta-lactams; Column: Phenyl-hexyl, 3.0×100 mm, 3 µm; Oven 35°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 40% B; Flow 0.50 mL/min. Observation: blank after high standard shows 0.5–2% residual peak.", "output": "Likely causes and actions:\n1) Strengthen needle wash (≥80% organic + 0.1% acid); extend wash cycles.\n2) Include a high-organic purge step between runs; evaluate backflush if available.\n3) Use low-adsorption vials/caps; check sample loop/syringe cleanliness.\n4) Set acceptance carryover <0.1% of high standard peak area.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "carryover", "detector": "UV", "instrument": "HPLC"}}
57
+ {"instruction": "Decide on ion-pairing conditions and caveats.", "input": "Matrix: cosmetics extract (IPA); Analytes: neutral pesticides; Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 35°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Gradient 2→40% B over 8 min; Flow 0.50 mL/min. Observation: basic amines tail at pH>6; poor retention at low organic.", "output": "Likely causes and actions:\n1) Try without ion-pair first using acidic pH and endcapped C18; assess tailing.\n2) If needed, test HFBA/TFA (UV penalty) or volatile IP reagents for MS with caution.\n3) Document equilibration burden and memory effects; dedicate system if adopting IP.\n4) Consider polar-embedded or mixed-mode columns as alternatives.\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "ion_pair", "detector": "PDA", "instrument": "HPLC"}}
58
+ {"instruction": "Diagnose bubble/degassing issues causing ripple.", "input": "Matrix: urine; Analytes: vitamins (B-group); Column: C18, 2.1×100 mm, 1.7 µm; Oven 40°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Gradient 2→95% B over 20 min; Flow 0.50 mL/min. Observation: periodic bubble bursts; UV shows spikes.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "degassing", "detector": "MS", "instrument": "UHPLC"}}
59
+ {"instruction": "Diagnose retention time drift >5%.", "input": "Matrix: food homogenate; Analytes: beta-lactams; Column: C8, 4.6×100 mm, 3.5 µm; Oven 40°C; A: water + 0.1% FA; B: ACN; Isocratic 30% B; Flow 1.0 mL/min. Observation: RT drifts +6–10% over a batch of 30 samples.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "RT_drift", "detector": "FLD", "instrument": "HPLC"}}
60
+ {"instruction": "Fix autosampler needle seat leak/cross-contamination.", "input": "Matrix: urine; Analytes: vitamins (B-group); Column: C8, 4.6×100 mm, 3.5 µm; Oven 25°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 60% B; Flow 1.0 mL/min. Observation: variable injection volumes; leaks near needle seat.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "autosampler", "detector": "UV", "instrument": "UHPLC"}}
61
+ {"instruction": "Resolve buffer pH/precipitation/mixing problems.", "input": "Matrix: pharma API in diluent; Analytes: PFAS (PFOS/PFOA); Column: C18, 2.1×100 mm, 1.7 µm; Oven 40°C; A: water + 0.1% FA; B: ACN; Gradient 2→40% B over 20 min; Flow 1.0 mL/min. Observation: salt precipitation suspected at high % organic.", "output": "Likely causes and actions:\n1) Use volatile buffers (ammonium formate/acetate) at ≤10–20 mM for MS; check pH at run temp.\n2) Avoid high-salt transitions to 100% organic; flush with salt-free aqueous before.\n3) Filter and degas buffers; replace after 48 h; verify miscibility A/B.\n4) At low pH for bases, add 0.1% FA; for strong tailing, consider 2–5 mM TFA (UV penalty).\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "buffer", "detector": "MS", "instrument": "UHPLC"}}
62
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: milk; Analytes: azo dyes; Column: C18, 2.1×100 mm, 1.7 µm; Oven 25°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 40% B; Flow 1.0 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "baseline", "detector": "PDA", "instrument": "HPLC"}}
63
+ {"instruction": "Ensure sufficient column equilibration between runs.", "input": "Matrix: tablet dissolution (pH 6.8); Analytes: sugars; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 45°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Isocratic 40% B; Flow 0.40 mL/min. Observation: first run after gradient shift differs from subsequent runs.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "equilibration", "detector": "PDA", "instrument": "HPLC"}}
64
+ {"instruction": "Resolve buffer pH/precipitation/mixing problems.", "input": "Matrix: milk; Analytes: glycosides; Column: PFP, 2.1×100 mm, 1.9 µm; Oven 40°C; A: water + 0.05% TFA; B: ACN; Gradient 5→95% B over 12 min; Flow 0.50 mL/min. Observation: salt precipitation suspected at high % organic.", "output": "Likely causes and actions:\n1) Use volatile buffers (ammonium formate/acetate) at ≤10–20 mM for MS; check pH at run temp.\n2) Avoid high-salt transitions to 100% organic; flush with salt-free aqueous before.\n3) Filter and degas buffers; replace after 48 h; verify miscibility A/B.\n4) At low pH for bases, add 0.1% FA; for strong tailing, consider 2–5 mM TFA (UV penalty).\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "buffer", "detector": "PDA", "instrument": "HPLC"}}
65
+ {"instruction": "Resolve pump pulsation/check valve problems.", "input": "Matrix: polymer extract; Analytes: neutral pesticides; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 40°C; A: water + 0.1% FA; B: ACN; Gradient 5→60% B over 12 min; Flow 1.0 mL/min. Observation: pulsation visible on pressure trace; peak area RSD ~6%.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "pump", "detector": "MS", "instrument": "UHPLC"}}
66
+ {"instruction": "Eliminate ghost peaks seen in solvent blanks.", "input": "Matrix: beer; Analytes: vitamins (B-group); Column: C18, 4.6×150 mm, 5 µm; Oven 25°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Isocratic 50% B; Flow 0.40 mL/min. Observation: ghost peaks in solvent blanks at random RTs.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "ghost_peaks", "detector": "PDA", "instrument": "HPLC"}}
67
+ {"instruction": "Resolve buffer pH/precipitation/mixing problems.", "input": "Matrix: leaf extract; Analytes: alkylbenzene sulfonates; Column: C18, 4.6×150 mm, 5 µm; Oven 40°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Gradient 10→40% B over 8 min; Flow 0.30 mL/min. Observation: salt precipitation suspected at high % organic.", "output": "Likely causes and actions:\n1) Use volatile buffers (ammonium formate/acetate) at ≤10–20 mM for MS; check pH at run temp.\n2) Avoid high-salt transitions to 100% organic; flush with salt-free aqueous before.\n3) Filter and degas buffers; replace after 48 h; verify miscibility A/B.\n4) At low pH for bases, add 0.1% FA; for strong tailing, consider 2–5 mM TFA (UV penalty).\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "buffer", "detector": "FLD", "instrument": "UHPLC"}}
68
+ {"instruction": "Eliminate ghost peaks seen in solvent blanks.", "input": "Matrix: food homogenate; Analytes: beta-lactams; Column: C18, 4.6×150 mm, 5 µm; Oven 35°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Isocratic 40% B; Flow 0.30 mL/min. Observation: ghost peaks in solvent blanks at random RTs.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "ghost_peaks", "detector": "UV", "instrument": "UHPLC"}}
69
+ {"instruction": "Fix peak tailing/broadening/splitting.", "input": "Matrix: groundwater; Analytes: beta-lactams; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 25°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Gradient 10→60% B over 12 min; Flow 1.0 mL/min. Observation: tailing factor ~1.8; occasional split peaks on main analyte.", "output": "Likely causes and actions:\n1) Match sample solvent to initial mobile phase; dilute strong solvents.\n2) Lower pH to suppress ionization for bases (pH 2.8–3.2) or raise for acids (pH 6.5–7.5).\n3) Consider polar-embedded/C18-AQ or PFP to reduce secondary interactions.\n4) Replace guard; check for column void (reverse flush briefly).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "peak_shape", "detector": "PDA", "instrument": "HPLC"}}
70
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: urine; Analytes: glycosides; Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 45°C; A: water + 0.1% FA; B: ACN; Isocratic 60% B; Flow 0.40 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "temperature", "detector": "FLD", "instrument": "HPLC"}}
71
+ {"instruction": "Resolve buffer pH/precipitation/mixing problems.", "input": "Matrix: urine; Analytes: sugars; Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 40°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 40% B; Flow 0.30 mL/min. Observation: salt precipitation suspected at high % organic.", "output": "Likely causes and actions:\n1) Use volatile buffers (ammonium formate/acetate) at ≤10–20 mM for MS; check pH at run temp.\n2) Avoid high-salt transitions to 100% organic; flush with salt-free aqueous before.\n3) Filter and degas buffers; replace after 48 h; verify miscibility A/B.\n4) At low pH for bases, add 0.1% FA; for strong tailing, consider 2–5 mM TFA (UV penalty).\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "buffer", "detector": "FLD", "instrument": "HPLC"}}
72
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: drinking water; Analytes: neutral pesticides; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 40°C; A: water + 0.05% TFA; B: ACN; Isocratic 35% B; Flow 0.50 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "temperature", "detector": "FLD", "instrument": "HPLC"}}
73
+ {"instruction": "Resolve buffer pH/precipitation/mixing problems.", "input": "Matrix: groundwater; Analytes: glycosides; Column: C18, 4.6×150 mm, 5 µm; Oven 45°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Isocratic 40% B; Flow 0.50 mL/min. Observation: salt precipitation suspected at high % organic.", "output": "Likely causes and actions:\n1) Use volatile buffers (ammonium formate/acetate) at ≤10–20 mM for MS; check pH at run temp.\n2) Avoid high-salt transitions to 100% organic; flush with salt-free aqueous before.\n3) Filter and degas buffers; replace after 48 h; verify miscibility A/B.\n4) At low pH for bases, add 0.1% FA; for strong tailing, consider 2–5 mM TFA (UV penalty).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "buffer", "detector": "UV", "instrument": "UHPLC"}}
74
+ {"instruction": "Diagnose bubble/degassing issues causing ripple.", "input": "Matrix: soil extract (MeOH); Analytes: vitamins (B-group); Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 35°C; A: water + 0.05% TFA; B: ACN; Gradient 5→95% B over 10 min; Flow 0.40 mL/min. Observation: periodic bubble bursts; UV shows spikes.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "degassing", "detector": "MS", "instrument": "UHPLC"}}
75
+ {"instruction": "Fix autosampler needle seat leak/cross-contamination.", "input": "Matrix: groundwater; Analytes: acidic herbicides (pKa 2–4); Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 40°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 40% B; Flow 0.30 mL/min. Observation: variable injection volumes; leaks near needle seat.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "autosampler", "detector": "PDA", "instrument": "UHPLC"}}
76
+ {"instruction": "Diagnose column frit clogging or void formation.", "input": "Matrix: wine; Analytes: basic amines (pKa 8–10); Column: C18, 4.6×150 mm, 5 µm; Oven 45°C; A: water + 0.05% TFA; B: ACN; Isocratic 30% B; Flow 0.50 mL/min. Observation: plate count dropped 40%; asymmetry 1.7; possible void.", "output": "Likely causes and actions:\n1) Check asymmetry and plates with a system suitability compound.\n2) Reverse-flush 5–10 min; replace guard; inspect for voids (shoulder/doublet).\n3) Reduce max pressure; verify fittings/ferrules to prevent channeling.\n4) Log column volumes; retire if performance out of spec.\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "column_health", "detector": "MS", "instrument": "UHPLC"}}
77
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: milk; Analytes: neutral pesticides; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 35°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 2→40% B over 8 min; Flow 1.0 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "baseline", "detector": "UV", "instrument": "HPLC"}}
78
+ {"instruction": "Diagnose bubble/degassing issues causing ripple.", "input": "Matrix: wine; Analytes: steroids; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 40°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Gradient 5→40% B over 20 min; Flow 1.0 mL/min. Observation: periodic bubble bursts; UV shows spikes.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "degassing", "detector": "MS", "instrument": "UHPLC"}}
79
+ {"instruction": "Address temperature-related retention and viscosity issues.", "input": "Matrix: plasma; Analytes: amino acids; Column: C8, 4.6×100 mm, 3.5 µm; Oven 40°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 2→40% B over 12 min; Flow 0.50 mL/min. Observation: RT shortens 3–5% when lab ambient increases; viscosity changes.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "temperature", "detector": "UV", "instrument": "UHPLC"}}
80
+ {"instruction": "Eliminate ghost peaks seen in solvent blanks.", "input": "Matrix: oil matrix (hexane); Analytes: azo dyes; Column: C18, 2.1×100 mm, 1.7 µm; Oven 45°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Gradient 2→60% B over 15 min; Flow 0.40 mL/min. Observation: ghost peaks in solvent blanks at random RTs.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "ghost_peaks", "detector": "UV", "instrument": "UHPLC"}}
81
+ {"instruction": "Decide on ion-pairing conditions and caveats.", "input": "Matrix: oil matrix (hexane); Analytes: PFAS (PFOS/PFOA); Column: C18, 2.1×100 mm, 1.7 µm; Oven 35°C; A: water + 0.05% TFA; B: ACN; Isocratic 30% B; Flow 0.40 mL/min. Observation: basic amines tail at pH>6; poor retention at low organic.", "output": "Likely causes and actions:\n1) Try without ion-pair first using acidic pH and endcapped C18; assess tailing.\n2) If needed, test HFBA/TFA (UV penalty) or volatile IP reagents for MS with caution.\n3) Document equilibration burden and memory effects; dedicate system if adopting IP.\n4) Consider polar-embedded or mixed-mode columns as alternatives.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "ion_pair", "detector": "FLD", "instrument": "HPLC"}}
82
+ {"instruction": "Diagnose bubble/degassing issues causing ripple.", "input": "Matrix: polymer extract; Analytes: phenols; Column: C8, 4.6×100 mm, 3.5 µm; Oven 30°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Isocratic 30% B; Flow 1.0 mL/min. Observation: periodic bubble bursts; UV shows spikes.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "degassing", "detector": "UV", "instrument": "HPLC"}}
83
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: QC standard in water; Analytes: neutral pesticides; Column: C18, 4.6×150 mm, 5 µm; Oven 45°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Isocratic 50% B; Flow 0.50 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "baseline", "detector": "PDA", "instrument": "HPLC"}}
84
+ {"instruction": "Stabilize noisy or drifting baseline.", "input": "Matrix: river water; Analytes: acidic herbicides (pKa 2–4); Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 45°C; A: water + 0.1% FA; B: ACN; Isocratic 60% B; Flow 0.40 mL/min. Observation: baseline ripple and drift during gradient; S/N reduced.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "baseline", "detector": "PDA", "instrument": "HPLC"}}
85
+ {"instruction": "Ensure sufficient column equilibration between runs.", "input": "Matrix: QC standard in water; Analytes: steroids; Column: PFP, 2.1×100 mm, 1.9 µm; Oven 25°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 50% B; Flow 0.40 mL/min. Observation: first run after gradient shift differs from subsequent runs.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "equilibration", "detector": "PDA", "instrument": "UHPLC"}}
86
+ {"instruction": "Diagnose retention time drift >5%.", "input": "Matrix: leaf extract; Analytes: bile acids; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 35°C; A: water + 0.1% FA; B: ACN; Gradient 2→95% B over 6 min; Flow 0.50 mL/min. Observation: RT drifts +6–10% over a batch of 30 samples.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "RT_drift", "detector": "UV", "instrument": "UHPLC"}}
87
+ {"instruction": "Fix autosampler needle seat leak/cross-contamination.", "input": "Matrix: river water; Analytes: alkylbenzene sulfonates; Column: C8, 4.6×100 mm, 3.5 µm; Oven 25°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Isocratic 30% B; Flow 1.0 mL/min. Observation: variable injection volumes; leaks near needle seat.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: carryover <0.1% of high standard; blank shows no analyte peaks.", "meta": {"topic": "autosampler", "detector": "MS", "instrument": "HPLC"}}
88
+ {"instruction": "Decide on ion-pairing conditions and caveats.", "input": "Matrix: wine; Analytes: caffeine analogs; Column: C18, 4.6×150 mm, 5 µm; Oven 25°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Gradient 10→80% B over 6 min; Flow 0.50 mL/min. Observation: basic amines tail at pH>6; poor retention at low organic.", "output": "Likely causes and actions:\n1) Try without ion-pair first using acidic pH and endcapped C18; assess tailing.\n2) If needed, test HFBA/TFA (UV penalty) or volatile IP reagents for MS with caution.\n3) Document equilibration burden and memory effects; dedicate system if adopting IP.\n4) Consider polar-embedded or mixed-mode columns as alternatives.\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "ion_pair", "detector": "MS", "instrument": "HPLC"}}
89
+ {"instruction": "Diagnose retention time drift >5%.", "input": "Matrix: cosmetics extract (IPA); Analytes: beta-lactams; Column: C18, 2.1×100 mm, 1.7 µm; Oven 35°C; A: water + 0.1% FA; B: ACN; Isocratic 35% B; Flow 0.50 mL/min. Observation: RT drifts +6–10% over a batch of 30 samples.", "output": "Likely causes and actions:\n1) Verify oven setpoint holds within ±0.1°C; allow ≥15 min stabilization.\n2) Increase post-gradient equilibration to ≥10–15 column volumes.\n3) Prime A/B 5 min to remove composition drift; verify mixer volume.\n4) Run bracketing standards every 10 injections; apply RT window ±2%.\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "RT_drift", "detector": "FLD", "instrument": "HPLC"}}
90
+ {"instruction": "Diagnose column frit clogging or void formation.", "input": "Matrix: drinking water; Analytes: bile acids; Column: C18, 4.6×150 mm, 5 µm; Oven 35°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Gradient 5→80% B over 20 min; Flow 0.40 mL/min. Observation: plate count dropped 40%; asymmetry 1.7; possible void.", "output": "Likely causes and actions:\n1) Check asymmetry and plates with a system suitability compound.\n2) Reverse-flush 5–10 min; replace guard; inspect for voids (shoulder/doublet).\n3) Reduce max pressure; verify fittings/ferrules to prevent channeling.\n4) Log column volumes; retire if performance out of spec.\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "column_health", "detector": "MS", "instrument": "UHPLC"}}
91
+ {"instruction": "Decide on ion-pairing conditions and caveats.", "input": "Matrix: groundwater; Analytes: phenols; Column: Phenyl-hexyl, 3.0×100 mm, 3 µm; Oven 40°C; A: 10 mM ammonium bicarbonate (pH 9.0); B: ACN; Gradient 10→95% B over 12 min; Flow 1.0 mL/min. Observation: basic amines tail at pH>6; poor retention at low organic.", "output": "Likely causes and actions:\n1) Try without ion-pair first using acidic pH and endcapped C18; assess tailing.\n2) If needed, test HFBA/TFA (UV penalty) or volatile IP reagents for MS with caution.\n3) Document equilibration burden and memory effects; dedicate system if adopting IP.\n4) Consider polar-embedded or mixed-mode columns as alternatives.\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "ion_pair", "detector": "UV", "instrument": "HPLC"}}
92
+ {"instruction": "Fix autosampler needle seat leak/cross-contamination.", "input": "Matrix: river water; Analytes: polyphenols; Column: C8, 4.6×100 mm, 3.5 µm; Oven 45°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Isocratic 35% B; Flow 1.0 mL/min. Observation: variable injection volumes; leaks near needle seat.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "autosampler", "detector": "PDA", "instrument": "HPLC"}}
93
+ {"instruction": "Troubleshoot high backpressure increase vs baseline.", "input": "Matrix: drinking water; Analytes: PFAS (PFOS/PFOA); Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 35°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 50% B; Flow 0.50 mL/min. Observation: pressure increased 30–50% vs yesterday; pulses under gradient.", "output": "Likely causes and actions:\n1) Bypass column to assess system backpressure; replace inline filter and frits (0.2 µm).\n2) Flush column: 50:50 water:organic (10 min) → 100% organic (10 min) → 100% water (10 min).\n3) Check solvent miscibility; remove salts before 100% organic to avoid precipitation.\n4) Inspect pump seals/check valves; verify degasser performance (vacuum level OK).\nAcceptance: backpressure within ±10% of baseline; plate count within spec.", "meta": {"topic": "pressure", "detector": "FLD", "instrument": "HPLC"}}
94
+ {"instruction": "Fix autosampler needle seat leak/cross-contamination.", "input": "Matrix: leaf extract; Analytes: sugars; Column: C18-AQ, 2.1×150 mm, 3 µm; Oven 25°C; A: 20 mM ammonium acetate (pH 6.8); B: ACN; Isocratic 40% B; Flow 1.0 mL/min. Observation: variable injection volumes; leaks near needle seat.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "autosampler", "detector": "FLD", "instrument": "HPLC"}}
95
+ {"instruction": "Fix peak tailing/broadening/splitting.", "input": "Matrix: food homogenate; Analytes: basic amines (pKa 8–10); Column: PFP, 2.1×100 mm, 1.9 µm; Oven 30°C; A: water + 0.1% FA; B: ACN; Gradient 10→80% B over 6 min; Flow 1.0 mL/min. Observation: tailing factor ~1.8; occasional split peaks on main analyte.", "output": "Likely causes and actions:\n1) Match sample solvent to initial mobile phase; dilute strong solvents.\n2) Lower pH to suppress ionization for bases (pH 2.8–3.2) or raise for acids (pH 6.5–7.5).\n3) Consider polar-embedded/C18-AQ or PFP to reduce secondary interactions.\n4) Replace guard; check for column void (reverse flush briefly).\nAcceptance: S/N > 10 at LOQ; baseline drift <5% over run.", "meta": {"topic": "peak_shape", "detector": "UV", "instrument": "UHPLC"}}
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+ {"instruction": "Resolve pump pulsation/check valve problems.", "input": "Matrix: polymer extract; Analytes: polyphenols; Column: HILIC amide, 2.1×100 mm, 1.7 µm; Oven 35°C; A: 50 mM ammonium formate (pH 3.2); B: MeOH; Gradient 5→60% B over 6 min; Flow 0.40 mL/min. Observation: pulsation visible on pressure trace; peak area RSD ~6%.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "pump", "detector": "UV", "instrument": "UHPLC"}}
97
+ {"instruction": "Troubleshoot high backpressure increase vs baseline.", "input": "Matrix: serum; Analytes: azo dyes; Column: C8, 4.6×100 mm, 3.5 µm; Oven 30°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Gradient 2→60% B over 15 min; Flow 0.50 mL/min. Observation: pressure increased 30–50% vs yesterday; pulses under gradient.", "output": "Likely causes and actions:\n1) Bypass column to assess system backpressure; replace inline filter and frits (0.2 µm).\n2) Flush column: 50:50 water:organic (10 min) → 100% organic (10 min) → 100% water (10 min).\n3) Check solvent miscibility; remove salts before 100% organic to avoid precipitation.\n4) Inspect pump seals/check valves; verify degasser performance (vacuum level OK).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "pressure", "detector": "FLD", "instrument": "HPLC"}}
98
+ {"instruction": "Fix autosampler needle seat leak/cross-contamination.", "input": "Matrix: tablet dissolution (pH 6.8); Analytes: alkylbenzene sulfonates; Column: Polar-embedded C18, 2.1×150 mm, 3 µm; Oven 40°C; A: 5 mM ammonium acetate in water (pH ~5.0); B: MeOH; Isocratic 40% B; Flow 0.30 mL/min. Observation: variable injection volumes; leaks near needle seat.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: Rs>1.5 for critical pair, asymmetry 0.9–1.3, %RSD RT <1.0%.", "meta": {"topic": "autosampler", "detector": "PDA", "instrument": "UHPLC"}}
99
+ {"instruction": "Diagnose bubble/degassing issues causing ripple.", "input": "Matrix: wine; Analytes: glycosides; Column: C18, 4.6×150 mm, 5 µm; Oven 25°C; A: 10 mM ammonium formate + 0.1% FA in water (pH ~3.2); B: ACN; Isocratic 30% B; Flow 0.50 mL/min. Observation: periodic bubble bursts; UV shows spikes.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: k′ between 2–10; tailing factor <1.3; RT window ±2%.", "meta": {"topic": "degassing", "detector": "FLD", "instrument": "UHPLC"}}
100
+ {"instruction": "Resolve pump pulsation/check valve problems.", "input": "Matrix: cosmetics extract (IPA); Analytes: alkylbenzene sulfonates; Column: C18, 2.1×100 mm, 1.7 µm; Oven 40°C; A: water + 0.05% TFA; B: ACN; Gradient 2→95% B over 6 min; Flow 0.40 mL/min. Observation: pulsation visible on pressure trace; peak area RSD ~6%.", "output": "Likely causes and actions:\n1) Run solvent and system blanks; isolate source by bypassing modules.\n2) Replace suspect solvents; ensure fresh buffer (≤48 h) and proper filtration (0.2 µm).\n3) Inspect degasser (vacuum level, tubing kinks) and pump check valves/seals.\n4) Perform needle-seat test, adjust wash, and verify no leaks (draw-air test).\nAcceptance: recovery 80–120%, precision (%RSD) ≤5% for n=6.", "meta": {"topic": "pump", "detector": "PDA", "instrument": "HPLC"}}
hplc_lora/README.md ADDED
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+ ---
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+ base_model: unsloth/llama-3-8b-Instruct-bnb-4bit
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+ library_name: peft
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+ pipeline_tag: text-generation
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+ tags:
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+ - base_model:adapter:unsloth/llama-3-8b-Instruct-bnb-4bit
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+ - lora
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+ - sft
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+ - transformers
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+ - trl
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+ - unsloth
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+ ---
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+
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+ # Model Card for Model ID
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+
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+ <!-- Provide a quick summary of what the model is/does. -->
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+
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+
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+
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+ ## Model Details
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+
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+ ### Model Description
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+
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+ <!-- Provide a longer summary of what this model is. -->
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+
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+
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+
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+ - **Developed by:** [More Information Needed]
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+ - **Funded by [optional]:** [More Information Needed]
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+ - **Shared by [optional]:** [More Information Needed]
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+ - **Model type:** [More Information Needed]
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+ - **Language(s) (NLP):** [More Information Needed]
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+ - **License:** [More Information Needed]
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+ - **Finetuned from model [optional]:** [More Information Needed]
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+
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+ ### Model Sources [optional]
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+
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+ <!-- Provide the basic links for the model. -->
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+
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+ - **Repository:** [More Information Needed]
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+ - **Paper [optional]:** [More Information Needed]
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+ - **Demo [optional]:** [More Information Needed]
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+
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+ ## Uses
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+
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+ <!-- Address questions around how the model is intended to be used, including the foreseeable users of the model and those affected by the model. -->
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+
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+ ### Direct Use
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+
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+ <!-- This section is for the model use without fine-tuning or plugging into a larger ecosystem/app. -->
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+
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+ [More Information Needed]
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+
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+ ### Downstream Use [optional]
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+
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+ <!-- This section is for the model use when fine-tuned for a task, or when plugged into a larger ecosystem/app -->
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+
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+ [More Information Needed]
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+
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+ ### Out-of-Scope Use
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+
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+ <!-- This section addresses misuse, malicious use, and uses that the model will not work well for. -->
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+
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+ [More Information Needed]
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+
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+ ## Bias, Risks, and Limitations
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+
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+ <!-- This section is meant to convey both technical and sociotechnical limitations. -->
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+
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+ [More Information Needed]
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+
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+ ### Recommendations
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+
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+ <!-- This section is meant to convey recommendations with respect to the bias, risk, and technical limitations. -->
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+
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+ Users (both direct and downstream) should be made aware of the risks, biases and limitations of the model. More information needed for further recommendations.
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+
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+ ## How to Get Started with the Model
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+
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+ Use the code below to get started with the model.
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+
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+ [More Information Needed]
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+
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+ ## Training Details
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+
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+ ### Training Data
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+
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+ <!-- This should link to a Dataset Card, perhaps with a short stub of information on what the training data is all about as well as documentation related to data pre-processing or additional filtering. -->
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+
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+ [More Information Needed]
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+
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+ ### Training Procedure
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+
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+ <!-- This relates heavily to the Technical Specifications. Content here should link to that section when it is relevant to the training procedure. -->
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+
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+ #### Preprocessing [optional]
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+
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+ [More Information Needed]
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+
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+
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+ #### Training Hyperparameters
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+
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+ - **Training regime:** [More Information Needed] <!--fp32, fp16 mixed precision, bf16 mixed precision, bf16 non-mixed precision, fp16 non-mixed precision, fp8 mixed precision -->
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+
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+ #### Speeds, Sizes, Times [optional]
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+
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+ <!-- This section provides information about throughput, start/end time, checkpoint size if relevant, etc. -->
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+
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+ [More Information Needed]
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+
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+ ## Evaluation
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+
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+ <!-- This section describes the evaluation protocols and provides the results. -->
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+
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+ ### Testing Data, Factors & Metrics
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+
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+ #### Testing Data
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+
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+ <!-- This should link to a Dataset Card if possible. -->
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+
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+ [More Information Needed]
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+
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+ #### Factors
124
+
125
+ <!-- These are the things the evaluation is disaggregating by, e.g., subpopulations or domains. -->
126
+
127
+ [More Information Needed]
128
+
129
+ #### Metrics
130
+
131
+ <!-- These are the evaluation metrics being used, ideally with a description of why. -->
132
+
133
+ [More Information Needed]
134
+
135
+ ### Results
136
+
137
+ [More Information Needed]
138
+
139
+ #### Summary
140
+
141
+
142
+
143
+ ## Model Examination [optional]
144
+
145
+ <!-- Relevant interpretability work for the model goes here -->
146
+
147
+ [More Information Needed]
148
+
149
+ ## Environmental Impact
150
+
151
+ <!-- Total emissions (in grams of CO2eq) and additional considerations, such as electricity usage, go here. Edit the suggested text below accordingly -->
152
+
153
+ Carbon emissions can be estimated using the [Machine Learning Impact calculator](https://mlco2.github.io/impact#compute) presented in [Lacoste et al. (2019)](https://arxiv.org/abs/1910.09700).
154
+
155
+ - **Hardware Type:** [More Information Needed]
156
+ - **Hours used:** [More Information Needed]
157
+ - **Cloud Provider:** [More Information Needed]
158
+ - **Compute Region:** [More Information Needed]
159
+ - **Carbon Emitted:** [More Information Needed]
160
+
161
+ ## Technical Specifications [optional]
162
+
163
+ ### Model Architecture and Objective
164
+
165
+ [More Information Needed]
166
+
167
+ ### Compute Infrastructure
168
+
169
+ [More Information Needed]
170
+
171
+ #### Hardware
172
+
173
+ [More Information Needed]
174
+
175
+ #### Software
176
+
177
+ [More Information Needed]
178
+
179
+ ## Citation [optional]
180
+
181
+ <!-- If there is a paper or blog post introducing the model, the APA and Bibtex information for that should go in this section. -->
182
+
183
+ **BibTeX:**
184
+
185
+ [More Information Needed]
186
+
187
+ **APA:**
188
+
189
+ [More Information Needed]
190
+
191
+ ## Glossary [optional]
192
+
193
+ <!-- If relevant, include terms and calculations in this section that can help readers understand the model or model card. -->
194
+
195
+ [More Information Needed]
196
+
197
+ ## More Information [optional]
198
+
199
+ [More Information Needed]
200
+
201
+ ## Model Card Authors [optional]
202
+
203
+ [More Information Needed]
204
+
205
+ ## Model Card Contact
206
+
207
+ [More Information Needed]
208
+ ### Framework versions
209
+
210
+ - PEFT 0.17.1
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huggingface_tokenizers_cache/.locks/models--unsloth--llama-3-8b-Instruct-bnb-4bit/348af68926a93e44c8f013e5d4d180b37a569570.lock ADDED
File without changes