Question: how to make 1.5 m tris buffer?
Answer: 1.5 M Tris, pH 6.8 (stock buffer for stacking gels) For 1 L • Dissolve 181.65 g Tris base in around 700 mL of ddH2O • Adjust the pH to 6.8 with concentrated HCl • Bring up the volume to 1 L with ddH2O Note: Make sure to let the solution cool down to room temperature before making the final pH adjustment.