Question: how much dtt to add to lysis buffer?
Answer: Prepare 1 mL of SDS lysis working buffer (100 mM Tris pH 6.8, 2% SDS, 300 mM NaCl, 50 mM DTT). The volume required will depend on the total number of cells for 10,000 cells per µL and may be more than 1 mL. a. Dissolve one vial of No Weigh DTT in 50 µl of lysis buffer stock.