Question: how much gdna for pcr?
Answer: Normally used concentration are 100-250 ng for mammalian genomic DNA and 20 ng for linearized plasmid DNA (circular plasmid DNA is slightly less efficiently amplified) per 50µl reaction. Concentration of dNTPs. The concentration of each dNTPs (dATP, dCTP, dGTP and dTTP) should be 200 µM.