Question: what is the difference between g and rpm in centrifugation?
Answer: g Force or Relative Centrifugal Force (RCF) is the amount of acceleration to be applied to the sample. It depends on the revolutions per minute (RPM) and radius of the rotor, and is relative to the force of Earth's gravity. ... Therefore, you have to convert g force (RCF) into revolutions per minute (rpms) and vice versa.