Question: how to use mfold software?
Answer: Find MFOLD on the web. Enter this URL for MFOLD: ... 
Then click the RNA Folding Form link.
Which will open up the following form: ... 
Optional Step. ... 
After a short wait, you will get this output page. ... 
ENERGY DOT PLOT. ... 
The dots in the box correspond to the indicated helical stem region in the structure. ... 
ENERGY DOT PLOT.