Question: how to prepare 1 m tris hcl ph 8.0?
Answer: To prepare a 1M stock solution of Tris-Cl: Dissolve 121 g Tris base in 800 ml H2O. Adjust to desired pH with concentrated HCl. Approximately 70 ml HCl is needed to achieve a pH 7.4 solution, and 42 ml for a pH 8.0 solution.