Question: what is the difference between tween 20 and triton x 100?
Answer: Triton is normally used in inmuno pp to isolate hydrophobic proteins; Tween 20 for lysis of cells and inmunoblots washes. The difference seems to be that Triton X-100 it has a phenolic ring that absorbs UV, can interfere in UV spectrometry to determinate proteins at 280 nm.