Question: how does tmb substrate work?
Answer: Abcam's TMB ELISA Substrate (High Sensitive) detects horseradish peroxidase (HRP) activity and contains 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution. ... When this substrate system is reacted with peroxidase, a soluble blue reaction product is obtained.