Question: what are the major differences between helicases topoisomerases and ligases used in dna replication?
Answer: What are the major differences between helicases, topoisomerases, and ligases used in DNA replication? a) Helicases and topoisomerases unwind DNA, with helicases functioning to relieve supercoil stress ahead of the replication process; and ligases seal DNA that has been broken or synthesized as Okazaki fragments.