Question: how to prepare 1 m tris buffer?
Answer: Solution A: Dissolve 121.14 g Tris (American Bioanalytical #AB14042) in 800 ml dH2O.
Adjust pH to 7.0 with the appropriate volume of concentrated HCl. Bring final volume to 1 liter with deionized water.
Autoclave and store at room temperature.