Question: what is the difference between np 40 and triton x-100?
Answer: NP-40 (Nonidet P-40) and Triton X-100 are milder, nonionic detergents. ... Proteins retain their native state in the presence of these detergents and protein-protein interactions can be preserved. These buffers can be used for co-IPs. NP-40 and Triton X-100 will not lyse nuclear membranes.