Question: what is the difference between tris base and tris hcl?
Answer: Tris-base 1M is water has pH around 11. ... Because in Tris-base you just add HCl to bring down its pH to 7, but in Tris-Hcl you have to add NaOH to bring pH to 7, although Tris-Hcl has HCl too. So it has more ionic strength(HCl + NaOH).