Question: what is the difference between conventional pcr and nested pcr?
Answer: Conventional PCR (C-PCR) has been used to detect specific target genes in various microorganisms (5, 6, 13). Nested PCR (N-PCR) was developed to improve sensitivity but can give erroneous positive results due to DNA contamination (1). ... Real-time quantitative PCR (Q-PCR) can detect V.