Question: how to do tlc count?
Answer: Count the cells in the Neubauer chamber. These are counted in the four large corner square labeled as W. is X number. One large area is 1 x 1 mm and depth is 0.1 mm. Total area counted in 4 large squares = 4 x 1 x o.1 = 0.4 µL. X x 10 / 4 is cell in 1 µL. ... 
Total TLC = counted cells x 50 = TLC/cmm.