A tecnica de SNaPshot e baseada na incorporacao de um unico nucleotide marcado com fluorescencia (ddNTP) a um fragmento de DNA nao marcado. Apos a reacao de SNaPshot o produto e misturado com formamida e um size standard e submetido a eletroforese capilar para identificacao dos fragmentos marcados com fluorescencia. Atraves desta tecnica, e possivel genotipar SNPs (Figura 1), identificar regioes metiladas, mapear BACs, entre outras aplicacoes. Veja abaixo alguns artigos com o uso dessa tecnica.Figura 1. SNaPshot labeling chemistry relies on single-base extension and termination. The SNaPshot Multiplex Kit uses a single-tube reaction to interrogate SNPs at known locations. The chemistry is based on the dideoxy single-base extension of an unlabeled oligonucleotide primer (or primers). Each primer binds to a complementary template in the presence of fluorescently labeled ddNTPs and DNA polymerase. The polymerase extends the primer by one nucleotide, adding a single ddNTP to its 3 end. The fluorescence color readout reports which base was added.SNaPshot Multiplex System for SNP genotyping.pdfVeja nesse Application Note uma lista de varios artigos que usaram a tecnica de SNaPshot para diferentes finalidades.Abstract: The SNaPshot Multiplex System is a primer extensionbased method developed for the analysis of single nucleotide polymorphisms (SNPs) (Figure 1). Through its multiplexing capability, up to 10 SNPs can be analyzed in a single reaction, regardless of their positions on the chromosome or the amount of separation from neighboring SNP loci. The ability to use unlabeled, user-defined primers allows researchers to incorporate SNPs of interest cost-effectively. The Multiplex Ready Reaction Mix (included in the system) helps ensure robust, reproducible analyses of multiplexed samples. Researchers can analyze more than 23,000 SNP genotypes per day on just one 3730xl Genetic Analyzer.Generated BAC Fingerprinting on Capillary Electrophoresis System.pdfWhite paper que descreve o uso da tecnica de Analise de Fragmentos para o mapeamento genicoAbstract: BAC fingerprinting provides an efficient and cost-effective method of characterizing large genomic fragment libraries for genome sequencing, positional cloning, and physical mapping efforts.High-throughput fingerprinting of bacterial artificial chromosomes using the snapshot labeling kit and sizing of restriction fragments by capillary electrophoresis.Abstract: We have developed an automated, high-throughput fingerprinting technique for large genomic DNA fragments suitable for the construction of physical maps of large genomes. In the technique described here, BAC DNA is isolated in a 96-well plate format and simultaneously digested with four 6-bp-recognizing restriction endonucleases that generate 3' recessed ends and one 4-bp-recognizing restriction endonuclease that generates a blunt end. Each of the four recessed 3' ends is labeled with a different fluorescent dye, and restriction fragments are sized on a capillary DNA analyzer. The resulting fingerprints are edited with a fingerprint-editing computer program and contigs are assembled with the FPC computer program. The technique was evaluated by repeated fingerprinting of several BACs included as controls in plates during routine fingerprinting of a BAC library and by reconstruction of contigs of rice BAC clones with known positions on rice chromosome 10.Capillary electrophoretic analysis of methylation status in CpG-rich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine.pdfAbstract: Polymerase-mediated single-base extension (SBE) of primers using a fluorescently labeled 20,30-dideoxynucleotide triphosphate terminator was