Patient description: 19 yo Hispanic female G1P1 at 32+ 6 weeks of gestational age presented to the OB clinic for routine follow up complaining of mild headache and leg swelling. Primary evaluation revealed BP of 146/99 and urine dipstick with 3+ proteins. Her BP and U/A were normal in previous visit. Repeat BP a few hours later is 150/100 mmHg. Laboratory studies showed a normal hematocrit, platelet count, and liver transaminase levels. She is complaining of fatigue but no fever or chills. She is also suffering of headaches with no vision changes. No shortness of breath, cough, chest pain, orthopnea and palpitations or skin rash were observed. Her physical exam was negative for abdominal pain, change in bowel habits, nausea, vomiting, dysuria, frequency, hematuria or frothy urine. Leg swelling was observed with no arthralgia or back pain. She has no specific past medical issues and only uses prenatal vitamins. Her family history is positive for DM type 2 and HTN. She is a social alcohol consumer with the negative history of smoking or drug use. She is only have one partner in past 2 years and didn't have any contraceptive methods since 2 years ago. Her BMI was 24 at the first

Clinical trial eligibility criteria: eligible ages (years): 18.0-100.0,  Endothelial Dysfunction Inflammation in Cardiopulmonary and Vascular Disease States Healthy Volunteers FOR WITH TO AND/OR AND/OR SUCH AS BUT NOT TO OTHER LUNG OR HEART 1. Males or females of age greater than or equal to 18 years old. 2. Subjects unable to provide informed consent must have a surrogate decision maker or another legally authorized representative (such as a legal guardian or holder of the DPA)  FOR WITH TO AND/OR AND/OR SUCH AS BUT NOT TO OTHER LUNG OR HEART 1. Hemoglobin <7.0 g/dL 2. Currently receiving infusion of any dosage of norepinephrine, vasopressin, or epinephrine; or dopamine at an infusion rate >2.5 micro g/kg/min 3. In the presence of known coronary artery disease (CAD) a systolic blood pressure (SBP) <90 mmHg. In the absence of known CAD a SBP <80 mmHg or mean arterial pressure (MAP) <60 mmHg. Research subjects may be excluded if in the opinion of the study investigators they have a condition that may adversely affect the outcome of the study or the safety of the volunteer. FOR WHO ARE TO CRRT (IRRESPECTIVE OF IN WHOM THE TOTAL OF BLOOD WILL NOT EXCEE 8 ML. 1. Males or females of age greater than or equal to 18 years old. 2. Subjects unable to provide informed consent must have a surrogate decision maker or another legally authorized representative (such as a legal guardian or holder of the DPA) FOR WHO ARE TO CRRT (IRRESPECTIVE OF IN WHOM THE TOTAL OF BLOOD WILL NOT 8 ML) 1. Hemoglobin <7.0 g/dL 2. In the presence of known coronary artery disease (CAD) a systolic blood pressure (SBP) <90 mmHg. In the absence of known CAD a SBP <80 mmHg or mean arterial pressure (MAP) <60 mmHg. FOR 1. Males or females of age greater than or equal to 18 years old 2. Must be able to provide informed, written consent FOR 1. Blood or platelet donation within the last 6 weeks. 2. Hemoglobin below normal (e.g. below 11.2 g/dl for females and below 13.7 mg/dl for males at the NIH CC); subjects may return for evaluation at a later date. (After initial enrollment, hematocrit does not need to be done prior to subsequent blood draws unless there is interval development of symptomatic anemia) 3. History of recreational drug use with the exception of marijuana (as long as marijuana use was >3 months from the time of study screening). 4. Active acute illness (i.e viral syndrome). Subjects may return for evaluation at a later date once the acute illness resolves. 5. Volunteers may be excluded if in the opinion of the study investigators they have a condition that may adversely affect the outcome of the study or the safety of the volunteer, such as, active tobacco use (> 6 months) in the past ten years, any tobacco use within 3 months prior to the screening evaluation, a history of clinically relevant and active cardiopulmonary disease (e.g. Including but not limited to any cardiopulmonary diseases not well controlled by medications, EKG with evidence of clinically relevant heart disease, COPD with hypoxemia and forced expiratory volume/forced vital capacity (FEV1/FVC) > 2 standard deviations from normal, etc.)