Patient description: Patient is a 34-year-old woman from Jordan who comes to clinic with some general and non-specific bones and joints pain. She is married and has 3 children. Her past medical and drug history are unremarkable. Her BMI is 23, BP: 120/75, HR: 75/min. Her laboratory study is remarkable for Vit D: 14ng/ml and otherwise healthy. (Ca: 9.2mg/dl, Phosphorus: 3.2mg/dl, PTH: 28pg/ml)

Clinical trial eligibility criteria: eligible ages (years): 18.0-75.0,  Inflammation Rheumatic Diseases Main 1. Capable of giving written informed consent, which includes compliance with study requirements and restrictions listed in the consent form. 2. Age requirements Cohorts 1 through 4 (healthy): Male or female age ≥ 18 years and ≤ 50 years Cohort 5 (AS): Male or female age ≥ 18 years and ≤ 75 years. 3. Females must agree to use a highly effective birth control method (<1% failure rate per year) throughout the study, reproductive status of non-childbearing based on medical history, or is postmenopausal Non-childbearing potential defined as pre-menopausal female with medical history of bilateral tubal ligation, bilateral oophorectomy (removal of the ovaries) or hysterectomy; hysteroscopic sterilization Postmenopausal defined as 12 months of spontaneous amenorrhea; with follicle stimulating hormone (FSH) confirmation Woman of Childbearing potential (WCBP) who is already using an established method of highly effective contraception or agrees to use one of the allowed BC methods for at least 28 days prior to the start of dosing (as determined by the Investigator Brochure or Investigator or designee) to sufficiently minimize the risk of pregnancy throughout study participation (until completion their study follow-up visit). 4. Males who are sexually active must agree to use one of the allowed birth control methods. Male subjects must also agree to sufficiently minimize the risk of pregnancy throughout study participation (until completion their follow-up visit). 5. Body Mass Index (BMI) Cohorts 1-4 (healthy): BMI 18.5 to 31 kg/m2 at Screening Cohort 5 (AS): BMI 18 to 33 kg/m2 at Screening Additional for Cohort 5 (AS) only: 6. AS diagnosis fulfilling the Modified New York for AS. 7. Active AS, as defined by the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) ≥ 4 despite NSAID, corticosteroid or DMARD therapy. 8. item 2, spinal pain score ≥ 4 (out of 10). 9. AS diagnosis confirmed by Sacroiliac (SI) imaging within the last 2 years (confirmed by central reader during Screening). 10. Does not have a history of complete spinal ankylosis. 11. CRP > ULN at Screening. 12. Two prior failures to NSAID therapy (sub-optimal clinical response following four weeks at maximum tolerated dose) or intolerance to NSAID therapy. Main  An individual will NOT be eligible for in this study if any of the following apply. 1. Clinically significant illness which required medical treatment within 8 weeks or a clinically significant infection within 4 weeks prior to Screening. 2. Disease diagnosis that may influence the outcome of the study; such as psychiatric disorders or disorders of the gastrointestinal tract, liver, kidney, respiratory system, endocrine system, hematological system, neurological system, cardiovascular system within 4 weeks prior to randomization, or individuals who have preexisting metabolic congenital abnormality(ies). 3. Positive Quantiferon test. 4. Use of prescription and non-prescription drugs Cohorts 1 through 4 (healthy): Use of prescription or non-prescription drugs, including vitamins, herbal and dietary supplements within 14 days or 5 half-lives (whichever is longer) prior to the first dose of study treatment, unless in the opinion of an Investigator and Medical Monitor the medication will not interfere with the study procedures or compromise subject safety or permitted under Section 5.10.1 Cohort 5 (AS) Current or prior treatment regimen that includes a biologic therapy (e.g., anti-TNF-alpha, anti-IL-17A, anti-IL-6, or anti-IL-12/23) Use of parenteral and/or intra-articular steroids or immunosuppressants within 6 weeks prior to the first dose of study treatment. Oral steroids are permitted if the dosage is ≤10 mg/day prednisone (or equivalent) and is stable for a minimum of 4 weeks before the first dose of study treatment and remains unchanged throughout the study. Also note: Oral NSAID treatment is permitted if the dosage is stable for a minimum of 2 weeks before the first dose of study treatment and remains unchanged throughout the study. Oral sulfasalazine treatment is permitted if the dosage is ≤ 3 g/d (max) and is stable for a minimum of 4 weeks before the first dose of study treatment and remains unchanged throughout the study. Oral methotrexate use is permitted if used for a minimum of 3 months, the dosage is ≤ 20 mg/week, and is the dose stable for a minimum of 4 weeks (including dosage of concomitant folate) before the first dose of study treatment and remains unchanged throughout the study. Subcutaneous methotrexate use is permitted if the dosage is ≤ 20 mg/week and is stable for a minimum of 4 weeks (including dosage of concomitant folate) before the first dose of study treatment and remains unchanged throughout the study. 5. Individual who received an investigational product (including placebo) 30 days prior to the start of dosing (5 half-lives or twice the duration of the biological effect of the investigational product), whichever is longer. 6. Weight loss or gain of >10% between screening and up to the start of dosing. 7. Hemoglobin level ≤ 12 g/dL at Screening. 8. Positive result for HIV (HIV-1/HIV-2 Antibodies), HBsAg or HCVAb screening tests. 9. Known or suspected history of drug abuse (amphetamines, barbiturates, cannabinoids, cocaine, opiates and phencyclidine) or alcohol misuse* within 6 months prior to Screening, or a positive urine drug test and/or alcohol breathalyzer test at Screening or baseline