Patient description: The patient is a 20 year old Caucasian female presents to the clinic with one-sided vision lost and facial weakness, dysarthria and numbness lasting for 1 day. She visited her PCP and underwent brain MRI which revealed a single plaque in the brainstem. After few months, she experienced lower extremities weakness led to balance problem. The second MRI revealed another lesion in the left cerebral hemisphere. The diagnosis of Relapsing Remitting Multiple Sclerosis (RRMS) is confirmed after the second MRI. Her past surgical history is significant for a C-section 2 years ago and she has a one child. She is divorced and is not currently in any sexual relationship. She smokes 10 cigarettes per day and drinks alcohol occasionally. She is working as an editor in a publisher company and she is happy that she can keep working from home most of the time. She is under the treatment of RRMS from 7 months ago.

Clinical trial eligibility criteria: eligible ages (years): 18.0-65.0,  Multiple Sclerosis Male and female subjects, 18-65 years of age, inclusive, at the time of informed consent signature 2. RRMS diagnosed according to the McDonald treatment naïve or currently using any of the FDA-approved DMDs (excluding natalizumab (Tysabri®), mitoxantrone or Rebif®) 3. Have a disease duration of up to twenty years 4. Be willing and able to comply with the study procedures for the duration of the trial 5. Have given written informed consent and signed Health Insurance Portability and Accountability Act (HIPAA) Authorization before any study related activities are carried out 6. Female subjects must not be either pregnant or breast-feeding and must lack childbearing potential, as defined by either: The following must be fulfilled by the Healthy Control subjects: 1. Male and female subjects, 18-65 years of age, inclusive, at the time of informed consent signature 2. Be willing and able to comply with the study procedures for the duration of the trial 3. Have given written informed consent and signed Health Insurance Portability and Accountability Act (HIPAA) Authorization before any study related activities are carried out 4. Female subjects must not be either pregnant or breast-feeding and must lack childbearing potential, as defined by either  Have received treatment within three months prior to Screening with interferon-beta-1a (Rebif®), IVIG or plasmapheresis 2. Have received treatment within thirty days prior to screening with immunosuppressant agents (e.g. including but not limited to mitoxantrone, cyclophosphamide, cladribine, fludarabine, cyclosporine or total body irradiation) or any other concomitant immunomodulatory therapies (e.g., azathioprine, methotrexate, CellCept®, natalizumab, alemtuzumab/Campath and other immunomodulators/monoclonal agents) 3. Have had a relapse within thirty days prior to the Screening Visit 4. Have received steroid treatment within thirty days prior to the initial MRI scan date at Study Day 1 5. Have inadequate liver function, defined by a alanine aminotransferase (ALT) > 2.5x upper limit of normal (ULN), or alkaline phosphatase > 2.5x ULN, or total bilirubin > 1.5x ULN 6. Have inadequate bone marrow reserve, defined as a total white blood cell count < 3.0x 109/L, platelet count < 75x109/L, hemoglobin < 100g/L 7. Have complete transverse myelitis or simultaneous-onset bilateral optic neuritis 8. Have a history of alcohol or drug abuse 9. Have thyroid dysfunction 10. Have moderate to severe renal impairment 11. Have a major medical or psychiatric illness that in the opinion of the investigator creates undue risk to the subject or could affect compliance with the study protocol 12. Have a history of seizures not adequately controlled by treatment 13. Have serious or acute cardiac disease, such as uncontrolled dysrhythmias, uncontrolled angina pectoris, cardiomyopathy, or uncontrolled congestive heart failure 14. Have, in the opinion of the investigator, any visual, physical or cognitive impairment that would preclude the subject from complying with the study protocol 15. Have a known hypersensitivity or allergy to interferon-beta or any of the excipients 16. Have received an investigational drug or experimental procedure within the past thirty days 17. Are pregnant or attempting to conceive The following must be fulfilled by the Healthy Control subjects: 1. Have met any of the above noted