Patient description: The patient is a 60-year-old Spanish man presenting with shortness of breath about a day before. The symptoms began acutely and progressively worsened. He is a known case of COPD since 2 years ago. The spirometry revealed post-bronchodilator FEV1/FVC = 60% of predicted values. He smokes 20 cigarette per day. His past medical history is remarkable for BPH and he is using Flomax for that. His family history is positive for HTN in his brother. His medication includes Duo-Neb inhaled q4 hr PRN, Vit D3 1000 units per day and Flomax for his PBH. He is an obese man who is acutely ill but oriented and conscious. The vital signs are as bellow: BP: 135/80 RR: 25/min HR: 75 bpm BMI: 40 O2sat: 90%

Clinical trial eligibility criteria: eligible ages (years): 18.0-75.0,  COPD Asthma for Healthy Smoking Subjects 1. Must have signed an informed consent indicating that they understand the purpose of and procedures required for the study and are willing to participate in the study. 2. Be between 18 and 75 years of age, inclusive, at informed consent. 3. Healthy as determined by a physician, based on medical history and physical examination. 4. Must have smoked regularly in the 12-month period preceding the screening visit and have a pack history of ≥ 5 pack years (number of pack years = number of cigarettes per day/20 x number of years smoked). for All COPD Subjects 1. Must have signed an informed consent indicating that they understand the purpose of and procedures required for the study and are willing to participate in the study. 2. Aged between 40 and 75 years of age inclusive, at the time of signing the informed consent. 3. COPD diagnosis: Subjects with a diagnosis of COPD as defined by the American Thoracic Society (ATS)/European Respiratory Society (ERS) guidelines (Celli, 2004). Symptoms must be compatible with COPD for at least 1 year prior to screening and post-bronchodilator spirometry readings at screening Post-bronchodilator FEV1/FVC ratio of <0.7 Post-bronchodilator FEV ≥40 % and ≤80 % of predicted normal values calculated using reference equations. Additional for Smoking COPD Subjects 1. Must have smoked regularly in the 12-month period preceding the screening visit and have a pack history of ≥ 5 pack years (number of pack years = number of cigarettes per day/20 x number of years smoked)  for Healthy Smoking Subjects Any potential subject who meets any of the following will be excluded from the participating study. 1. Upper or lower respiratory tract infection within 4 weeks of the screening visit. 2. Positive test for alcohol at screening. 3. Taking prescription medication in the 14 days before screening. 4. Subjects whose primary consumption of tobacco is via methods other than cigarettes (manufactured or self-rolled). Primary methods of tobacco consumption that are excluded but are not limited to pipes, cigars and e-cigarettes. 5. Subjects who are unable to produce a total weight of at least 0.1 grams (g) of selected sputum at screening 6. Urinary cotinine levels at screening < 30 ng/ml. 7. Subject is mentally or legally incapacitated. 8. Subject is an employee of the Sponsor or contract research organization (CRO), or a relative of an employee of the Sponsor or CRO. 9. Any other reason that the Investigator considers makes the subject unsuitable to participate for COPD Subjects Any potential subject who meets any of the following will be excluded from the participating study 1. Upper or lower respiratory tract infection within 4 weeks of the screening visit. 2. Positive test for alcohol at screening. 3. Subjects who are unable to produce a total weight of at least 0.1g of selected sputum at screening. 4. Subject is mentally or legally incapacitated. 5. Subject is an employee of the Sponsor or contract research organization (CRO), or a relative of an employee of the Sponsor or CRO. 6. Any other reason that the Investigator considers makes the subject unsuitable to participate. 7. A history of life-threatening COPD including Intensive Care Unit admission and/or requiring intubation. 8. Taking theophylline in the 28 days before screening 9. Change in COPD medication in the 28 days before screening 10. Taking oral steroids in the 28 days before screening Additional Criterion for Smoking COPD Subjects 1. Subjects whose primary consumption of tobacco is via methods other than cigarettes (manufactured or self-rolled). Primary methods of tobacco consumption that are excluded but are not limited to pipes, cigars and e-cigarettes. 2. Urinary cotinine levels at screening < 30 ng/ml. Additional Criterion for Ex-Smoking COPD Subjects 1. The subject is a smoker (regular or irregular), or has smoked or used nicotine-containing products within the 6 months prior to screening