Patient description: The patient is a 32-year-old obese woman who came to the clinic with weight concerns. She is 165 cm tall and her weight is 113 kg. She is complaining of sleep apnea, PCO and dissatisfaction with her body shape. She is a high-school teacher married for 5 years. She doesn't use any contraceptive methods for the past 4 months and she had no prior pregnancies. She doesn't smoke or use any drugs. She likes to try diets and exercise to lose weight. She completed the four square step test in 14 seconds. Her BP: 130/80, HR: 195/min and her BMI is: 41.54. Her labs: FBS: 98 mg/dl TG: 150 mg/dl Cholesterol: 180 mg/dl LDL: 90 mg/dl HDL: 35 mg/dl Her cardiac assessment is normal. Her joints and ROM are within normal.

Clinical trial eligibility criteria: eligible ages (years): 18.0-45.0,  Glucose Metabolism Disorder Male or female aged 18 2. Weight stable for at least 3 months prior to screening (< 5% weight change) 3. BMI of 21 4. Cohort 1 Aspartame Consumers: Primary dietary sweetener is aspartame; consumes at least 36 ounces of diet soda in which aspartame is a primary ingredient (Diet Coke or Diet Dr Pepper) a day for at least 6 months duration prior to study enrollment (Primary dietary sweetener is aspartame" should be defined as: "840g per week sucrose equivalent from aspartame and less than 280 grams per week sucrose equivalent from all other sweeteners (non-caloric and caloric sweeteners). For reference, one 12oz soda is 40g sucrose equivalent, and one teaspoon (one packet) of artificial sweetener is 4g sucrose equivalent.)" Cohort 2 Aspartame Naïve Participants: Consumes less than or equal to 2 cans of diet soda per week 5. Screening Visit #2 OGTT Outcome h plasma glucose < 110 mg/dl AND h plasma glucose < 140 mg/dl 6. Abnormal OGTT during the screening phase of the study (area under curve on the high end of distribution) 7. Must be able to comply with a metabolic Prudent diet 8. Willing to fast 10 hours before each OGTT and BodPod 9. Current level of exercise (aerobic and/or resistance training) must be able to be sustained while an in-patient  Any evidence of US National Cholesterol Education Program Adult Treatment Panel III Clinical Identification of the Metabolic Syndrome (must have 3 or more of the following risk factors): Abdominal Obesity, given as a waist circumference Men >102 cm (>40 in) Women > 88 cm (>35 in) Triglycerides >150 mg/dl HDL Cholesterol Men < 40 mg/dl Women <50 mg/dl Blood Pressure >130/ >85 mm Hg Fasting Glucose > 110 mg/dl 2. Average systolic blood pressure (SBP) > 150 mmHg and / or diastolic blood pressure (DBP) > 90 mmHG (based on 3 BPs taken at screening visit #1), 3. LDL-C > 240mg/dl 4. Triglycerides > 400 mg/dl 5. Evidence of a liver disorder (ALT > three fold of the ULN) 6. Evidence of any renal disease 7. Currently on a weight-loss diet 8. Diabetes 9. Self-reported history of thyroid dysfunction 10. Sugar sweetened beverage consumption (> 84 ounces per week) 11. Hemoglobin <13.0 g/dl for males and 12.0 g/dl for females 12. Current, prior (within 2 months), or anticipated use of any of the following medications: antihyperlipidemic, hyperglycemic medications, Antineoplastics, Antiretrovirals, Selective Serotonin Reuptake Inhibitors, Diuretics, Antihypertensives, Anticonvulsants, Hormone therapy, Birth control 13. Self-reported antibiotic use within the previous 3 months 14. Currently pregnant or lactating 15. History of cardiac disease 16. Active illegal drug user (self-reported) 17. History of GI surgery (gastric bypass, bariatric, Roux-en-Y, colon resection, etc.) 18. Habitual ingestion of > 2 alcoholic beverages/day 19. Ever diagnosed with an eating disorder (self-reported) 20. Use of steroids or beta agonists (orally, intranasal or inhaled) within a week of any OGTT 21. Positive Hepatitis Serology (Hep. B surface Antibody; Hep. B surface antigen; Hep. C surface antibody) 22. HIV Positive 23. Tobacco use within the past 3 months 24. Any medical or social condition that, in the opinion of the Investigator, might pose additional risk to the participant or confound the results of the study