Patient description: The patient is a 60-year-old Spanish man presenting with shortness of breath about a day before. The symptoms began acutely and progressively worsened. He is a known case of COPD since 2 years ago. The spirometry revealed post-bronchodilator FEV1/FVC = 60% of predicted values. He smokes 20 cigarette per day. His past medical history is remarkable for BPH and he is using Flomax for that. His family history is positive for HTN in his brother. His medication includes Duo-Neb inhaled q4 hr PRN, Vit D3 1000 units per day and Flomax for his PBH. He is an obese man who is acutely ill but oriented and conscious. The vital signs are as bellow: BP: 135/80 RR: 25/min HR: 75 bpm BMI: 40 O2sat: 90%

Clinical trial eligibility criteria: eligible ages (years): 28.0-80.0,  Chronic Obstructive Pulmonary Disease Asthma for all subjects: 1. Subject's written informed consent obtained prior to any study-related procedure; 2. Ability to understand the study procedures, the risks involved and ability to demonstrate correct use of the inhaler using the AIM™ (Aerosol Inhalation Monitor) Vitalograph® 3. Body Mass Index (BMI) between 18 and 32 kg/m2 extremes inclusive; 4. Good physical status, determined on the basis of the medical history and a general clinical examination, at screening; 5. Vital signs within normal limits: Diastolic BP 40-90 mmHg, Systolic BP 90-140 mmHg or 90-160 mmHg if >45 yrs 6. Males fulfilling one of the following 1. Males with non-pregnant Women of childbearing potential (WOCBP) partners: they and/or their partner of childbearing potential must be willing to use a highly effective birth control method in addition to the male condom from the signature of the informed consent and until 90 days after the follow-up visit. Subjects must not donate sperm during the study and for 90 days after the follow-up visit or 2. Males with pregnant WOCBP partner: they must be willing to use male contraception (condom) from the signature of the informed consent and until 90 days after the follow-up visit. Subjects must not donate sperm during the study and for 90 days after the follow-up visit or 3. Non-fertile male subjects (contraception is not required in this case) or 4. Males with partner not of childbearing potential (contraception is not required in this case). 7. WOCBP fulfilling one of the following 1. WOCBP with fertile male partners: they and/or their partner must be willing to use a highly effective birth control method with low user dependency from the signature of the informed consent and until 30 days after the follow-up visit or 2. WOCBP with non-fertile male partners (contraception is not required in this case). 8. Females of non-childbearing potential defined as physiologically incapable of becoming pregnant (i.e. post-menopausal or permanently sterile). Tubal ligation or partial surgical interventions are not acceptable. If indicated, as per investigator's request, post-menopausal status may be confirmed by follicle-stimulating hormone levels (according to local laboratory ranges) 9. 12 -lead digitised Electrocardiogram (12-lead ECG) considered as normal at screening and at Day -1 Additional only for Healthy Volunteers and Asthmatic patients: 1. Male and female subjects aged 28-55 years inclusive; 2. Non or ex-smokers who smoked < 5 pack years (pack-years = the number of cigarette packs per day times the number of years) and stopped smoking > 6 months prior to screening; Additional only for Healthy Volunteers: 1. Lung function measurements within normal limits at screening: FEV1 equal to or more than 80% of predicted Additional only for Asthmatic patients: 1. Diagnosis of asthma: Established diagnosis of permanent asthma for at least 12 months according to GINA guidelines 2. Patients with a pre-bronchodilator 60%≤ FEV1 < 80% of the predicted normal value 3. Patients with a documented reversibility defined as an increase ≥ 12% and 200mL over baseline within 30 min after inhalation of 400µg salbutamol pMDI Additional only for COPD patients: 1. Male and female patients aged 40-80 years inclusive 2. A smoking history of at least 10 pack-years 3. Current or ex-smokers are eligible. 4. Established diagnosis of COPD 5. A post-bronchodilator FEV1 ≤ 50% of the predicted normal value and a post-bronchodilator FEV1/FVC < 0.7 10-15 minutes after 4 puffs (4x100 µg) of Salbutamol pMDI  for all subjects: 1. Pregnant or lactating women; 2. Clinically relevant and uncontrolled respiratory, cardiac, hepatic, gastrointestinal, renal, endocrine, metabolic, neurologic, or psychiatric disorders 3. Clinically relevant abnormal laboratory values at screening suggesting an unknown disease and requiring further clinical investigation 4. Subjects with medical diagnosis of narrow-angle glaucoma, prostatic hypertrophy or bladder neck obstruction 5. Positive HIV1 or HIV2 serology at screening 6. Blood donation or blood loss less than 2 months prior screening 7. Participation to another clinical trial where investigational drug was received and last investigations were performed less than 90 days prior to screening; 8. Documented history of alcohol abuse within 12 months prior to screening or a positive alcohol breath test 9. Documented history of drug abuse within 12 months prior to screening 10. Positive results from the Hepatitis serology which indicates acute or chronic Hepatitis B or Hepatitis C at screening 11. Subjects who have cardiovascular condition such as, but not limited to unstable ischemic heart disease, NYHA Class III/IV left ventricular failure, acute ischemic heart disease in the last year prior to study screening, which may impact the safety of the subject or the evaluation of the result of the study according to the Investigator's judgment 12. Unsuitable veins for repeated venepuncture/cannulation 13. Intake of non-permitted concomitant medications in the predefined period prior to screening 14. Radiation exposure, including that from the present study, in the last 12 months 15. Known intolerance/hypersensitivity or contra-indication to treatment Additional only for Healthy volunteers and Asthmatic patients: 1. Positive urine test for cotinine at screening or prior Day 1 2. Current use of any nicotine or nicotine replacement product Additional only for Asthmatic and COPD patients: 1. Use of systemic corticosteroids medication within 4 weeks prior to the screening or slow release corticosteroids within 12 weeks before the screening or prior Day 1 2. A diagnosis of lung cancer or a history of lung cancer 3. A history of lung volume reduction surgery 4. Lower respiratory tract infection that requires use of antibiotics Additional only for Healthy volunteers: 1. Subjects with history of breathing problems Additional only for Asthmatic patients: 1. History of near fatal asthma, hospitalization for asthma in intensive care unit 2. Any change in dose, schedule or formulation in the treatment of asthma in the 4 weeks prior to the screening visit or prior Day 1 3. Non-persistent asthma: 4. Treatment with chronic systemic corticosteroids or anti IgE or other monoclonal antibodies 5. Known respiratory disorders other than asthma Additional only for COPD patients: 1. Any change in dose, schedule or formulation in the treatment for COPD in the 4 weeks prior to the screening 2. A concurrent diagnosis of asthma, in the opinion of the investigator 3. Known respiratory disorder other than COPD that in the Investigator's opinion would affect efficacy and safety evaluation or place the patient at risk. 4. Oxygen therapy: patients requiring long term (at least 12 hours daily) oxygen therapy for chronic hypoxemia. 5. Change in dose or type of any medications for COPD within 4 weeks prior to the screening visit