Patient description: The patient is a 47-year-old Asian woman complaining of persistent feelings of sadness. She lost interest in activities she used to enjoy. She states that her mood is mostly depressed for the past 3 weeks. She also lost her appetite , which led to about 5kg weight loss. She complains of loss of energy and feelings of worthlessness nearly every day. She is not using any drugs and she does not smoke. She doesn't drink alcohol. She used to exercise every day for at least 30 min. But she doesn't have enough energy to do so for the past 3 weeks. She also has some digestive issues recently. She is married and has 4 children. She is menopausal. Her husband was recently diagnosed with colon cancer and he is starting his chemotherapy. There is nothing remarkable in her past medical history and her drug history is only positive for Vit D3 1000 units daily. Her family history is negative for any psychologic problems. Her HAM-D score is 20.

Clinical trial eligibility criteria: eligible ages (years): 21.0-65.0,  Healthy Volunteers Alcohol Drinking Related Problems Alcohol-Related Disorders Stage 1: 1. 21 to 65 years old 2. Healthy volunteers only: Consuming on average 7 or less standard drinks/week if female; 14 or less standard drinks/week if male (as determined by the most recent measurement within the past 90 days Alcohol Timeline Followback) 3. AUD participants only: Diagnosed with current moderate to severe alcohol use disorder according to most recent SCID 5 diagnosis  Significant history of head trauma or cranial surgery 2. History of neurological disease based on self-report and neuromotor physical exam, conducted by a health care provider, that would interfere with neuroimaging research. Posthoc, clinical MRI scans done according to NIH Clinical Center policy may be reviewed and if there is evidence from that scan of past or current neuroabnormalities that, in the PI or MAI s expert opinion, interfere with research neuroimaging data, the subject may be excluded from data analysis. 3. Physical health concern that would significantly impair or increase the risk of study participation. 4. Healthy volunteers only: Have fulfilled DSM-5 for a current substance or alcohol use disorder 5. Female participants only: Currently pregnant 6. Presence of any ferromagnetic objects in the body that may be aversively affected by or contraindicated for MRI as determined by the NIAAA MRI Safety Screening Questionnaire of the screening protocol 14-AA-0181 7. Any flag on the NIAAA MRI Safety Screening Questionnaire, unless cleared by medically responsible staff (MD/NP) 8. History of non-substance related psychosis 9. Lack of experience with alcohol (defined as less than 3 lifetime drinks Stage 2: 1. 21 to 65 years old 2. Inpatient currently seeking treatment for alcohol use disorder Significant history of head trauma or cranial surgery, 2. History of neurological disease based on self-report and neuromotor physical exam, conducted by a health care provider, that would interfere with neuroimaging research. 3. Physical health concern that would significantly impair or increase the risk of study participation. 4. Presence of any contraindication for fMRI as determined by the NIAAA MRI Safety Screening Questionnaire of the screening protocol 14-AA-0181 5. Any flag on the NIAAA MRI Safety Screening Questionnaire, unless 6. History of non-substance related psychosis 7. Female participants only: Currently pregnant