Patient description: A 17 year old boy complains of vomiting, non-bloody diarrhea, abdominal pain, fever, chills and loss of appetite for the past 3 days. He ate a salad at a restaurant prior to his diarrhea onset. Physical exam was remarkable for pallor, jaundice, and diffuse abdominal tenderness. Lab results were as follows: Hemoglobin: 9.7 g/dL Platelet: 110,000 /cu.mm Creatinine: 3.6 mg/dL blood urea nitrogen (BUN): 73 mg/dL direct bilirubin: 2.4 mg/dL lactate dehydrogenase (LDH): 881 IU/L (normal: 110-265 IU/L) Peripheral blood smear showed a moderate number of schistocytes and helmet cells. Shiga-like toxin-producing E. coli (STEC) stx1/stx2 were found in stools. He has no other underlying disease and he is not on any medications.

Clinical trial eligibility criteria: eligible ages (years): 18.0-49.0,  Enterotoxigenic Escherichia Coli Infection Diarrhea Healthy male and non-pregnant female subjects aged 18 to <50 years; 2. BMI of 19.0 to 35.0 kg/m2 3. Willingness to participate after informed consent has been obtained from the subject prior to any study related procedures. 4. Completion of a training session and demonstration of comprehension of the protocol procedures and knowledge of ETEC-associated illness by passing a written examination. 5. If subject is of childbearing potential: 1. Negative pregnancy test at screening with understanding to not become pregnant within 28 days after challenge; 2. Subject has practiced an effective method of contraception during the 30 days before screening (Visit 0); 3. Subject agrees to employ adequate birth control measures for the duration of the study  Participated in research involving investigational product within 30 days before planned date of first vaccination or planned use through Day 44; 2. Any prior exposure to ETEC (including LSN03-016011/A) or cholera occupationally or received LT (Or any mutant forms of LT (e.g., LTR192G, LTR192GL211A), ETEC, or cholera vaccine); 3. Subjects with known abnormal stooling patterns (fewer than 3 per week or more than 3 per day); 4. Known allergies to any component of the vaccine; 5. Subjects with known allergies to more than 1 planned antibiotics: 6. History of diarrhea while traveling in a developing country within the last 3 years; 7. Subjects whose occupation involves handling of ETEC or cholera bacteria; 8. Women who are pregnant or breastfeeding; 9. Significant medical conditions including chronic, immunosuppressive, malignant, or gastrointestinal diseases (e.g. History of Irritable Bowel Syndrome (as defined by the Rome III or medical diagnosis) or gastric ulcer disease) or enteric, pulmonary, cardiac, liver or renal disease. Some medical conditions which are adequately treated and stable may be acceptable in the study (e.g. hypertension); 10. Significant abnormalities in screening lab hematology or serum chemistries; 11. Use of any medication known to effect the immune system (e.g. systemic corticosteroids) within 30 days of vaccination or planned use during active study period (excluding inhaled steroids); 12. Evidence of confirmed infection with HIV, Hepatitis B or Hepatitis C; 13. Subjects with IgA (Immunoglobulin A) deficiency (serum IgA < 7 mg/dl or limit of detection of assay); 14. Regular use of antacids, antidiarrheal, loperamide, bismuth subsalicylate, diphenoxylate or similar medication less than 2 weeks prior to enrolling in the study and through the inpatient portion of the study; 15. Known or suspected alcohol abuse or illicit drug use within the last year, positive urine toxicology for drugs of abuse; 16. Persons who are committed to an institution (by virtue of an order issued either by the judicial or the administrative authorities); 17. Persons who are in a dependent relationship with the sponsor, an investigator or other study team members, or the study center. 18. Any other which, in the investigator's opinion, would compromise the ability of the subject to participate in the study, the safety of the study, or the results of the study