Talk:Total internal reflection fluorescence microscope

message: Please simplify the language. -- Toytoy 00:47, Nov 19, 2004 (UTC)
Removed : 200 nm is less than one-third of the wavelength of red light. Not relevant Cnickelfr 
:It does seem relevant in that normally the axial resolution is limited by the wavelength of illumination.  But I don't know whether what you removed specifically was useful or not...  --169.230.5.59 18:03, 13 May 2005 (UTC)
thread_title: Comments by Toytoy

message: Is this the same as Epifluorescence microscopy? 'cause it sounds simillar.
--hydkat 10:07, 14 March 2006 (UTC)
thread_title:  Epifluorescence microscopy? 

message: I think the following comment is technically incorrect:
"Importantly, the fluorescence excitation energy of the evanescent wave is the same as the energy of the wavelength of the light that was totally internally reflected."
The intensity of light that penetrates into the lower index medium is actually a function of the angle of incidence that the illumination light strikes the interface, as well as the distance above the the interface in the lower medium.  Is the text trying to stress that the wavelength of the illuminating light does not change upon total internal reflection? I don't see why this is so important, and there is no reason to think that it would be altered in the first place.  I suggest this text be removed and new text that goes into some of the finer details of electromagnetic waves at a dielectric interface be added instead.
--Johnoreo 02:07, 2 October 2006 (UTC)
thread_title:  Importantly...? 

message: There is a TIR page and this TIRFM page. But wouldn't it be nice for the overall structure if there was a page entitled TIRF? That one would explain TIRF in general as a spectroscopic tool with some more theoretical detail ,e.g. penetration depth, intensity of the evanescent wave, dependence of incident angle, etc.
TIRFM is only one specific TIRF technique, i.e. TIRF imaging. Probably it is the most important one but there are other non-imaging techniques like TIRF sensing or TIRF-FCS.
I agree that TIRF does not always imply imaging. Most notably, TIRF spectroscopy has been going on since the 70's.  There is also another related technique, TIR microscopy (not fluorescence) that images the scatter light of particles in the prism geometry.  Perhaps they should be distinguished as well. 
142.150.76.90 20:34, 30 November 2006 (UTC)
thread_title:  TIRF is missing 

message: While the new diagrams are definitely an improvement, there are two things that I would like to see changed:
1. Objective-based TIRF is usually done in the inverted microscope configuration. So the picture should be flipped vertically
2. Fluorescence emission in ray diagrams is usually colored to indicate a redshift.  In these diagrams, the illuminating light color and the fluorescence light color should be swapped.
142.150.76.90 20:34, 30 November 2006 (UTC)
thread_title:  The new diagrams 

message: The objective can be placed on either side of the specimin
Also the laser can be shone from the plane the glass or from the side of the glass
http://www.olympusmicro.com/primer/techniques/fluorescence/tirf/tirfconfiguration.html
http://www.microscopyu.com/articles/fluorescence/tirf/tirfintro.html —The preceding unsigned comment was added by 84.64.130.245 (talk) 15:58, 9 December 2006 (UTC)
thread_title:  Different setups 

message: I don't see why the reference to Andre et al. regarding single-molecule imaging is relevant, the paper only has 5 citations according to Google Scholar. I added another reference with 500+ citations which seems to be more relevant. If somebody knows something better, go ahead.131.211.45.92 (talk) 16:38, 16 January 2012 (UTC)
thread_title:  Relevant citation? 

message: I prepared an image :File:Tirfm numbers like TIRFM2.svg, which uses the same numbers as TIRFM2.svg to simplify caption of both images together. Just for your information. -- Pemu (talk) 10:33, 4 August 2016 (UTC)
thread_title:  Image 

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