lflage/wiki-talks huggingface dataset:
Talk:Malate–aspartate shuttle

message: The opening paragraph says that NADH carries the "reduction equivalent" of a hydrogen ion. I find it confusing. NADH is the reduced form of NAD+. A hydride anion is the "reduced equivalent" of a proton (or a hydrogen cation). It is clearer to state that NADH formally carries a hydride anion. Since the hydride anion is too strong of a Bronsted base to exist in water, it is carried as part of NADH.
--Chibibrain (talk) 06:59, 22 November 2009 (UTC)
thread_title: NADH formally carries a hydride anion

message: The article says malate starts in the cytosol but the diagram shows it in the intermembrane space. How does it get there from the cytosol?  —Preceding unsigned comment added by 94.173.122.171 (talk) 16:32, 18 April 2010 (UTC)
replies: text: Unlike the inner membrane, the outer membrane is not a barrier to small metabolites, which freely exchange between cytoplasm and intermembrane space.96.54.32.44 (talk) 01:18, 14 December 2010 (UTC)
thread_title:  cytosol? 

message: This same pathway is used for reductive carboxyloation of pyruvate to oxaloacetate in the matrix of the mitochondria then transfer of malate and subsequent oxidation back to oxaloacetate during gluconeogenesis.  Should a section be included here? `````  — Preceding unsigned comment added by 69.138.157.32 (talk) 17:31, 4 November 2012 (UTC)
thread_title:  Gluconeogenesis 

message: I do not see why, at physiologic pH that the diagram would show an ionized hydroxyl group on the malate molecule.   — Preceding unsigned comment added by 172.78.56.88 (talk) 16:39, 22 February 2017 (UTC)
thread_title:  Negative Charge on Hydroxy group of Malate? 
Talk:Memory B cell

message: Encyclopedic articles should give an introduction to a topic, or at least have an introductory section.
This article reads like someone copied lingo out of a text book without knowing what the words and concepts really mean.  Not knowing what the words really mean means being unable to translate into language people outside the field will understand. 50.71.80.195 (talk) 12:43, 23 December 2014 (UTC)
thread_title:  Reads more like a 3 or 4th year university text book than an encyclopedia 

message: I removed some unsourced material:
Memory B cell response on first, and second infections of a virus.1. Antigens from the virus are brought into the body, and are then accepted by the receptors on the B cells. 2. The B cells accept the antigens, and then start to allow the antigens to change the antibodies and the B cell. 3. The B cells either change into memory B cells, or create plasma cells that secrete the newly changed antigens. 4. On the second infection when that same viruses antigens are present the memory B cells recognize this virus. This causes a much larger response from these memory B cells compared to the first response of the B cells. 5. Since there was a much larger response from the Memory B cells this causes more plasma cells to be produced as a response. When there are more abundant plasma cells, more antibodies are then produced as a result of this.
--Malerooster (talk) 02:51, 3 June 2015 (UTC)
thread_title:  Image and material 

message: Hello. Over the next few weeks I will be working on this page by adding information from reliable sources and organizing information. Here are some of the sources that I plan on using: 
1. Seifert, M., Küppers, R. Human memory B cells. Leukemia 30, 2283–2292 (2016). https://doi.org/10.1038/leu.2016.226
2. Taylor, Justin J., Marc K. Jenkins, and Kathryn A. Pape. "Heterogeneity in the differentiation and function of memory B cells." Trends in immunology 33.12 (2012): 590-597.
3.Phan, Tri Giang, and Stuart G. Tangye. "Memory B cells: total recall." Current opinion in immunology 45 (2017): 132-140.
4. McHeyzer-Williams, Louise J., and Michael G. McHeyzer-Williams. “ANTIGEN-SPECIFIC MEMORY B CELL DEVELOPMENT.” Annual Review of Immunology, vol. 23, no. 1, Apr. 2005, pp. 487–513, 10.1146/annurev.immunol.23.021704.115732. 
5. Shinnakasu, Ryo, and Tomohiro Kurosaki. “Regulation of Memory B and Plasma Cell Differentiation.” Current Opinion in Immunology, vol. 45, Apr. 2017, pp. 126–131, 10.1016/j.coi.2017.03.003. Accessed 29 Jan. 2021.  — Preceding unsigned comment added by Immcarle144 (talk • contribs) 05:31, 29 January 2021 (UTC)
thread_title:  Editing and cleaning up the Memory B cell page
Talk:Fake blog

message: This page really needs to be expanded. I'm sure there's stuff I'm unaware of which could be added. Cheers, Lankybugger 21:28, 14 February 2007 (UTC)
replies: text: Merged the contents of Flog blog here per the What Links Here, which showed Flog linking here and not to Flog blog. Cheers, Lankybugger 17:20, 15 February 2007 (UTC)
thread_title: Untitled

message: There is another category of fake blogs: randomly generated blogs -- using random text generators. For example,  and  are two examples of supposed blog communities which are using this script  to generate their entire websites. Their purpose is not clear, although probably it has something to do with online advertisement. -- Pichote 22:33, 18 October 2007 (UTC)
replies: text: The article Spam blog covers the randomly generated blogs and could be added here as a see also. --MetaEd (talk) 20:53, 6 October 2010 (UTC)
thread_title: Random generated blog communities

message: This article covers two quite different meanings of the term 'fake blog': blogs created by corporations or other organisations which give the illusion of independent grassroots support (also called 'astroturfing'), and parody blogs created to mock a person or group, or simply for humour value. One key difference being that the latter type is usually extremely obviously fake and meant to be seen as such, while the former is meant to be seen as 'real'. I think these concepts are so different they should be covered in two different articles; one possibility would be to split this article, or perhaps the material about the first type should be merged into the astroturfing article while the material about the second type (which seems to be the more popular meaning) stays here. Robofish (talk) 00:26, 24 April 2010 (UTC)
replies: text: It might also be useful to distinguish between fake blogs that are promotional of the brand or personality supposedly behind them (ie positive), and fake blogs that are created to ridicule or criticise the author (ie: negative)? (I have been trying to improve the brandjacking article and have made this distinction). Paul W (talk) 13:46, 13 July 2010 (UTC)
thread_title:  Two quite different meanings 

message: — Assignment last updated by Kdowning2 (talk) 16:33, 19 March 2024 (UTC)
thread_title: Wiki Education assignment: Writing 10 - Both Classes
Talk:Metabolic engineering

message: This page is being update as part of a class project for the senior Chemical Engineering elective, Metabolic Engineering at the A. James Clark School of Engineering at University of Maryland, College Park. 
Reviews:
I added a few citation needed to the article. 
Major Concerns:
The term 'metabolic engineering' emerged in the 1990’s to describe bacteria that had been genetically engineered using the strategy of removing, one by one, the most restrictive bottlenecks in a metabolic pathway and the recombinant DNA technology.
^ This sentence is not a clear definition, and the end of it doesn't make sense. For the history possiby mention that the first book was published by Stephanopoulous in 199X and a dedicated journal began publication in 199X.
"As the world population is growing, oil, food and energy prices are likely to climb. The challenge is not to stop using tires, eating or buying oil and clothes . Rather, it’s about developing alternative, more sustainable ingredients from which to produce the products we rely on. Recent advances in metabolic engineering are helping us prepare for and meet these most pressing challenges."
^ This sounds like a call to arms, or an advertisement not an encyclopedia.
The example of Metabolic Flux Analysis hardly reads like an encyclopedic entry. It isn't very clear to follow either if I had no idea what was going on.  In simple terms, the goal is to calculate theoretical yield, which absent of the network would be 6/7, (85.7%) but with network constraints including cofactor balances it becomes 3/7   Then with a modified network, it increases back to 6/7. The goal of the analysis is finding the best way to remove the bottlenecks. It also involves implementing the proposed changes, and analyzing how the cell responds, which looking at the paper shows that it does not operate anywhere near the theoretical yield, which you do not mention.
Now since PYR no longer leaves the cell, DAHP now gets 6 moles for every seven moles of glucose that eneters the cell.
^ Pyr never leaves the cell, it leaves the system you are analyzing.
The last section is good, and should probably be placed above the example.  It needs more refinement, and more links.
Mfconway (talk) 19:30, 25 November 2011 (UTC)
thread_title: Untitled

message: My name is baraa  I'm a master's student at uskuder university and this is my final exam assignment Braa Elwaleed (talk) 14:00, 24 January 2023 (UTC)
thread_title:  Hello Wikipedia
Talk:Microbody

message: Lysosomes do not belong to microbodies!
Microbodies are: peroxisomes, glyoxysomes, glycosomes and Woronin bodies.
Glyoxysomes are in filamentous fungi and plants and besides peroxisomal enzymes, they harbor two key enzymes of the glyoxylate cycle: isocitrate liase and malate synthase. Glycosomes are found in kinetoplastida like Trypanosoma and additionally to peroxisomal enzymes they house glycolysis enzymes.
Woronin bodies is a separate story. They do not possess any enzyme, they are filled with only one protein called HEX1 which forms hexagonal crystals and thus whole organelle gets hexagonal shape. Woronin bodies seal septal pores to protect the cell filaments from cytoplasmic bleeding and death during the mechanical injury.
Dmanagadze 12:10, 5 May 2007 (UTC)
replies: text: Then this should be explained as to WHY they do not belong to microbodies and WHO defined this. 2A02:8388:1641:8380:8920:7EEB:D50A:6466 (talk) 16:29, 27 September 2019 (UTC)
thread_title: [Untitled]

message: I am editing this page please do not re edit
thank youJoshgatz1994 (talk) 13:06, 18 March 2013 (UTC)
thread_title: [Untitled2]

message: Is a lysosome not a microbody as well? If not, which definition is applied here and why does it exclude lysosomes?
I am not absolutely certain but I think that lysosomes are also microbodies. Either way I think it would help to explain whether they are NOT, if they are not; right now the article is a bit ... sparse. 2A02:8388:1641:8380:8920:7EEB:D50A:6466 (talk) 16:23, 27 September 2019 (UTC)
thread_title:  Lysosomes
Talk:Microsome

message: this information on cytochrome p450 is misleading to the point of being incorrect and needs rewording.  From my understanding Cytochrome p450 is in cell membranes, and while cytochrome p450 can be in a microsome, this implies that it is only in the cytoplasm.  I don't think that this information is correct and seriously needs to be reviewed by an expert.  However, I am no where near an expert, I just see conflicting information with this an other sources by pharmogolists discussing this matter - but I haven't found a written form to cite.
**The information is correct, microsomes are fragments of ER that contain P450s. P450s are typically found in either the ER or the mitochondria (so it is true that not all P450s are in the ER). The microsomes contain more than just P450s (for example UGTs involved in conjugative reactions among others).  —Preceding unsigned comment added by 158.135.7.57 (talk) 14:55, 9 September 2008 (UTC)
thread_title: Untitled
Talk:Green High School (Franklin Furnace, Ohio)

message: This sentence seems to have been inadvertently copied from the other Green High School. TonyTheTiger 23:46, 15 February 2007 (UTC)
thread_title:  Dubious sentence 
Talk:Mitotic index

message: Jparcoeur (talk) 14:58, 4 November 2014 (UTC)

message: Cell growth occurs when it goes through interphase and mitosis to complete the cell cycle. Most cells when mature, begin to lose the ability to divide; while others begin dividing rapidly. For a group of cells that lose the ability to divide and rarely complete the cell cycle, a high proportion of cells in the resting stage of the cell cycle (G1) is expected. In a rapidly dividing cell population, a high proportion of cells in the stage of mitosis is to be expected. One way to quantify cell division is by using the mitotic index; number of cells in mitosis divided by the total number of cells. Quantifying a dividing cell populations can show how cells differ in their capability to divide. 
Cblan001 (talk) 01:57, 5 November 2014 (UTC)
thread_title: Untitled

message: \mbox{Mitotic I}=\frac\mbox{(P+M+A+T)}\mbox{N}*100%
, (P+M+A+T) —  the sum of all cells in phase as prophase, metaphase, anaphase and telophase, respectively; N — total number of cells.
Cblan001 (talk) 01:38, 5 November 2014 (UTC)
thread_title:  Formula
Talk:Müller glia

message: I do not see a good reason why the redirect goes from Müller glia to Muller glia, rather than the other way round, given that they are named after Heinrich Müller (physiologist). -- Daniel Mietchen (talk)  01:16, 24 December 2013 (UTC)
thread_title:  Page name 

message: Millar cell function 2409:4063:210F:D815:0:0:1EA0:B0AC (talk) 13:42, 13 July 2022 (UTC)
thread_title:  Ocular anatomy
Talk:Myelocyte

message: Are they a single cell type in the granulocytic series, or all of them ?
Haematopoiesis says "All blood cells are divided into three lineages.
*Erythroid cells ...
*Lymphocytes are ...
* Myelocytes, which include granulocytes, megakaryocytes and macrophages and are derived from common myeloid progenitors, are involved in such diverse roles as innate immunity, adaptive immunity, and blood clotting. This is myelopoiesis.
- Rod57 (talk) 14:03, 28 December 2017 (UTC)
thread_title:  Confusing intro
Talk:Myofilament

message: Article starts with "Myofilaments are the two protein filaments of myofibrils", but the rest of the article refers to "three different types of myofilaments".
Permacultura (talk) 13:41, 5 April 2022 (UTC)
replies: text: Hello Permacultura - it's one of the many issues (everywhere) waiting to be addressed (by someone). Most sources refer to two and just a few include the elastic. It could probably be overcome by stating that the two are usually referred to as being the filaments that slide over in muscle contraction and bring in the third type. OR just stick to the cited three and change the lead. which imo is the better option.  Thanks --Iztwoz (talk) 18:42, 5 April 2022 (UTC)
replies: text: You're probably right, User:Iztwzo . I'm studying Physiology 1 now, so I'll check out a few of the current textbooks, and see what I come up with Permacultura (talk) 06:42, 14 April 2022 (UTC)
thread_title: Article contradicts itself: How many types are there? 2 or 3?
Talk:NFAT

message: Source ideas for this page 
Macián, F., López-Rodríguez, C., & Rao, A. (2001). Partners in transcription: NFAT and AP-1. Oncogene, 20(19), 2476–2489. https://doi.org/10.1038/sj.onc.1204386
Medyouf, H., & Ghysdael, J. (2008). The calcineurin/NFAT signaling pathway: a novel therapeutic target in leukemia and solid tumors. Cell Cycle (Georgetown, Tex.), 7(3), 297–303. https://doi.org/10.4161/cc.7.3.5357
Nguyen, T., & Di Giovanni, S. (2008). NFAT signaling in neural development and axon growth. International Journal of Developmental Neuroscience: The Official Journal of the International Society for Developmental Neuroscience, 26(2), 141–145. https://doi.org/10.1016/j.ijdevneu.2007.10.004
Pan, M.-G., Xiong, Y., & Chen, F. (2013). NFAT Gene Family in Inflammation and Cancer. Current Molecular Medicine, 13(4), 543–554. https://doi.org/10.2174/1566524011313040007
Qin, J.-J., Nag, S., Wang, W., Zhou, J., Zhang, W.-D., Wang, H., & Zhang, R. (2014). NFAT as cancer target: mission possible? Biochimica Et Biophysica Acta, 1846(2), 297–311. https://doi.org/10.1016/j.bbcan.2014.07.009
Rao, A., Luo, C., & Hogan, P. G. (1997). Transcription factors of the NFAT family: regulation and function. Annual Review of Immunology, 15, 707–747. https://doi.org/10.1146/annurev.immunol.15.1.707
Immcarle63 (talk) 21:49, 5 February 2018 (UTC)
thread_title: Untitled
Talk:Brit Awards 2007

message: Why does the sound keep dubbing out? Is this to prevent obscenities being broadcast? Oliver Davison 21:32, 14 February 2007 (UTC)
replies: text: I don't think so, as it cut out during the Oasis preformance --84.68.56.195 21:53, 14 February 2007 (UTC)
replies: text: During the credits it said it was to cover strong language. Bit stupid during a song, and they didn't get the language anyway. --84.68.56.195 22:00, 14 February 2007 (UTC)
replies: text: "Possibly as a result of this, bad language was carefully censored from the show." This isn't very accurate, as a swear did get through. Also, though this is my opinion, the producers seemed to over do it a bit... --84.68.56.195 22:15, 14 February 2007 (UTC)

text: Not realising thia WP:TALK. There it clearly states that 'Talk pages are not for general conversation', so having now realised that I have breached this I apolopgise if I have broken the guidelines. I think (although not seemingly it already has) this heading should be stoppedOliver Davison 18:44, 19 February 2007 (UTC)
thread_title:  Sound Problem 

message: Is this really needed? —The preceding unsigned comment was added by 84.68.56.195 (talk) 22:00, 14 February 2007 (UTC)
thread_title:  Is record label winners needed?
Talk:Nucleoprotein

message: I am improving this page for a class project under the supervision of Dr. Lesly Temesvari (Username: LTEMESV) at Clemson University (Clemson, SC, USA).Pzhang2 (talk) 22:36, 15 September 2015 (UTC)
thread_title: Untitled

message: It would make a lo f sense to have all the Nucleoprotein information on the one page. Deoxyribonucleoprotein and Ribonucleoprotein could sensibly be merged into this page to collate the information. If the sections got too large at some later stage they could be re-split. Honestly, DNA-binding protein and Protein–DNA interaction could conceivably be merged in too. Any opinions? T.Shafee(Evo&Evo)talk 12:23, 15 June 2017 (UTC)
replies: text: I certainly support merging Nucleoprotein, Ribonucleoprotein and Deoxyribonucleoprotein to eliminate massive redundancy and overlap.  I think DNA-binding protein is a different thing, though, as I don't think "nucleoprotein" is normally used for transcription factors and proteins involved in DNA replication and so on, except in the context of experiments involving short DNA fragments.  DNA-binding protein and Protein–DNA interaction could probably be merged with each other, though. Adrian J. Hunter(talk•contribs) 02:23, 14 April 2018 (UTC)
replies: text:  Good points. I've gone ahead and done the Deoxyribonucleoprotein-Ribonucleoprotein-Nucleoprotein merge as well as the DNA-binding protein-Protein–DNA interaction. Also added a redirect from empty Protein–RNA interaction to RNA-binding protein. T.Shafee(Evo&Evo)talk 11:46, 14 April 2018 (UTC)
thread_title:  Merge suggestion
Talk:Nutrient sensing

message: Great start on the article! One thing that stuck out to me was on the plant section. Elaborate on "higher plants," I understand what you mean, but others may not.   — Preceding unsigned comment added by Spencer.Schulz (talk • contribs) 05:11, 22 May 2017 (UTC)
thread_title: Untitled

message: I am a graduate student working on nutrient sensing and signaling. I aim to update this page with specific differences in mechanisms of nutrient sensing between lower (eg. yeast) and higher (plants and multicellular) eukaryotes. I may also add a section on bacterial nutrient sensing eventually.  — Preceding unsigned comment added by Amogh.jalihal (talk • contribs) 05:59, 21 June 2016 (UTC)
thread_title:  Making this page more current 

message: The article is well well written, and I like the citations and how they are active links. There was one source where the link was broken or not working. In the "Types of Nutrients in Plants" section, you have K & P, it be nice to somehow mark that they're symbols from the periodic table, or you can simply spell out the compound for any non scientific reader. I think it would be great if the brain and gut section had some more information, and maybe you're looking into that? Other than that the article is legit and I didn't notice any grammatical errors.  — Preceding unsigned comment added by Dawood34 (talk • contribs) 06:20, 22 May 2017 (UTC)
thread_title:  Peer Review.
Talk:Oogonium

message: This article seems to refer to Homo sapiens - or rather the femal of... How are the oogonia/oögonia of the algae (e.g. Fucus) to be included/sorted out? —The preceding unsigned comment was added by Osborne (talk • contribs) 14:05, 19 June 2007 (UTC)
thread_title: Algae and other forms of life
Talk:Graiguenamanagh

message: With all respect to the photographer, this primarily shows a man, a dog, and a boat.  Something that shows the town proper or part of it would be better I think.  --BrokenSphere 21:36, 14 February 2007 (UTC)
replies: text: As the person from the town believes it should be "Village of the monks" and , and  all seem to agree I am changing it to reflect this. (Okeeffe.christopher (talk) 03:24, 14 February 2008 (UTC)
thread_title: Better picture?
Talk:County Carlow

message: The statement in the new lead which was inserted into the county articles is completely misleading; County Carlow is one of the 32 traditional counties - yes. But the traditional county is not co-locational with modern Carlow County Council. Sarah777 (talk) 12:12, 9 April 2011 (UTC)
thread_title: The new lead

message: In the infobox the population is given as 56875 (census 2016), but the source if of the census 2011. In the main article the population is given as 54812 (census 2011) - outdated. And no source given.--Wanfried-Dublin (talk) 19:09, 8 January 2017 (UTC)
thread_title:  Wrong source for population 
Talk:River Barrow

message: The Irish name is An Bhearú!! (acc. to Gasaitéar na hÉireann, logainm.ie) I've never seen Barra anywhere. Bearb(h)a is the old spelling.    It is the same name as the River Bervie in Scotland.
Cmconraoi (talk) 20:44, 9 April 2010 (UTC)
thread_title:  Irish name 

message: :What does "Most Prominent" mean? The Barrow is marginally longer than the Suir, but has a much smaller flow rate, about the same as the Nore. The River Suir flowing through Waterford City hardly looks less prominent than the Barrow (at any stage). I will remove the "Most Prominent" statement.Johnnyf1nn (talk) 22:23, 10 November 2014 (UTC)
replies: text: Needs expanding; references. Sarah777 (talk) 20:59, 18 June 2011 (UTC)
replies: text: Well, we have not defined "Barrow" sufficiently. From what I can see the Barrow is the main river. The estuary is called "the Barrow Estuary" or "the Waterford Estuary." Considering the Barrow near its source, it runs downstream to the south. It is joined by the Nore from the right, which makes the Nore a right-bank tributary. The Nore ENDS there. The Barrow continues south made more prominent by the waters of the Nore. Now from the right comes the Suir. That makes the Suir another right-bank tributary. The Suir ends at the junction. The Barrow goes on to the sea more prominent than either the Suir or the Nore. The prominence of the Suir IS the prominence of the Barrow below the junction. We endorse this view in the box by calling the Nore and the Suir tributaries. I got no doubt that the Suir is more prominent than the upper Barrow, but that is not the point. The mighty Barrow Estuary beats all the tributaries in prominence. Barrow forever! Hip, hip .... But now, the "Three Sister" terminology is somewhat confusing to me. Are they to be considered one sister at a time independently or as a united trinity of sisters? Frankly it sounds a bit Celtic and a bit pagan to me. As has been suggested above I think this local usage should be explained. I'm very confused. Perhaps we might start with what and where is the Barrow.Botteville (talk) 02:29, 30 May 2016 (UTC)
thread_title:  Most Prominent?