Talk:Sex-determining region Y protein

message: BASEDbio (talk) 20:06, 7 October 2013 (UTC)

message: It would be helpful if you could elaborate on how the SRY gene gets expressed, leading to the production of TDF, as well as the type of signaling pathway that TDF is involved in (specifically leading to primary sex cord development). It gets a little confusing/misleading in the second paragraph with the use of the words gene and protein; "This gene is a DNA-binding protein that enhances other transcription factors, or is a transcription factor itself." It would be helpful if you could clarify that the gene (SRY) codes for a protein (TDF) which then leads to the transcription of the primary sex cords mRNA by some signaling pathway.
BASEDbio (talk) 23:30, 3 October 2013 (UTC)
thread_title:  Suggestion 

message: Sorry for butting in here just before the start of this course assignment.  But I think it would worthwhile to discuss this merger proposal before rather than after this assignment starts.
This article is about the Testis determining factor protein while the SRY article is about the gene encoding this protein.  Because the subject of the gene and the protein are so interrelated, I think it makes more sense to have one article that discusses both. For same reason, the vast majority of the other ~10,000 Gene Wiki articles are about both the protein and the gene encoding that protein. Hence I think these two articles should be merged.  Thoughts? Boghog (talk) 20:29, 9 October 2013 (UTC)
thread_title:  Merger proposal 

message: Philippe B, Hawkins RJ, Sinclair AH, Taylor A, Griffiths BL, Goodfellow PN, and Fellous M. “Genetic evidence equating SRY and the testis-determining factor.” Human Molecular Genetics, Imperial Cancer Research Fund, Lincoln’s Inn Fields, London, UK. Nature: Vol. 348, 29 November 1990. <http://www.bio.davidson.edu/Courses/Molbio/restricted/01GenSRY/1990_evidence.pdf> 
Harley VR, Clarkson MJ, Argentaro A. The molecular action and regulation of the testis-determining factors, SRY (sex-determining region on the Y chromosome) and SOX9 [SRY-related high-mobility group (HMG) box 9]. Prince Henry's Institute of Medical Research, Victoria, Australia. 2003 Aug: 24(4):466-87. NCBI. PubMed. <http://www.ncbi.nlm.nih.gov/pubmed/12920151>
“Sex Determining Region Y.” GeneCards. The Human Gene Compendium. <http://www.genecards.org/cgi-bin/carddisp.pl?gene=SRY>
“SRY_HUMAN.” Reviewed, UniProtKB/Swiss-Prot. Uniprot. <http://www.uniprot.org/uniprot/Q05066#section_ref>
Nia2993 (talk) 02:18, 15 October 2013 (UTC)
thread_title:  Bibliography of Relevant Research 

message: The article will include both TDF and SRY as one topic (see "merger proposal" for reasons). A section devoted to the SRY gene will detail the region of DNA that SRY is found on (the Y chromosome), the transcription/regulatory factors that control expression of the SRY gene, the signaling pathways that these factors use to activate SRY, and any post-transcriptional modifications leading to the mRNA that codes for TDF. The next section will focus on Testes Determining Factor (the SRY protein). Emphasis will be placed on the function of TDF as a transcription factor. This will include the signaling pathway(s) it is involved in, other transcription factors that it interacts with, and the genes that these transcription factors regulate. The next section will describe how the activation of these genes (by TDF and its associated transcription factors) result in maleness/testes formation. This will include a description of the proteins these genes code for and how they ultimately lead to the development of testes/the male sex organs. The final section will briefly discuss the disorders that result from a malfunction in this extensive signaling pathway.  — Preceding unsigned comment added by BASEDbio (talk • contribs) 04:56, 18 October 2013 (UTC)
thread_title:  Summary of Article to Come 

message: Responsibility for the development of the Gene Regulation section. Pitpeelorchard (talk) 19:02, 18 November 2013 (UTC)
thread_title:  Member Contribution 

message: Overall the article is really great! There is a lot of good information and all the major points seemed to be covered thoroughly.  You also have a lot of wiki-links to other articles, which was really helpful. I found myself using some of them from time to time to make sure I knew exactly what was going on.  One suggestion I have is for the flow of the introduction.  When I was reading the first paragraph I was questioning about SRY and wished I had known more about that before delving into the TDF. Maybe conjoining the two paragraphs or adding a short introduction to SRY first might be helpful to the flow, rather than explaining TDF and then going back and explaining SRY, since there is no link to a separate article for SRY.  Also, you have an image for SRY, but is there an image for TDF specifically? That might be helpful as well. I really liked how you set up your next three sections. I thought that the flowed well into each other.  I liked how you started out by telling the audience that SRY is hard to study and slightly complicated, however I was curious about what types of mutations cause sex reversal at the end of that paragraph. Is there a way you could link to a wiki article on sex reversal, or find out anything about these mutations to just add a quick line about it?  In the second gene regulation paragraph, I had a few questions about the genes and binding site names.  I know that WT1 has a wiki-link, but are there wiki-links to the other two, SP1 and SF1? I found myself getting a little lost through that section and could use a little more explanation about the SF1, SP1, and WT1 elements. What exactly are they? What do they stand for? Also in that paragraph, I thought the part about DAX regulation seemed rushed. Is there any more information on that? How does DAX interact with this process, since it hasn’t been talked about in the previous sections before?  The evolution paragraph was great but I almost wish it was early in the article, maybe in the introduction or before gene regulation? That helped me put it into perspective and if you leave it there that’s fine, but personally I think it would have helped me earlier.  The introduction to the Function section was perfect in my opinion. It made sense. It flowed well. And it had links when I needed them.  I just noticed that there weren’t any references cited in this paragraph and I don’t know if that was your paragraph or one that came with the article but it might be a good idea to add some references if you can.  In Action in the nucleus, the sentences flowed well and the information wasn’t too overwhelming. I think this was also a good paragraph. I just have a question about NLS and what it is, but that’s very minor.  Again, your SOX9 paragraphs flowed well.  There were a few bits of information that I wished I had known earlier, like the SF1 protein information and function, but overall I didn’t find too many problems with these paragraphs. I thought your function sections were very well done.  The Sex Influences sections were also well done.  I really liked how you included the different syndromes and mutations with the SRY and sex development. I think the title of the next part may improve if you change it slightly. After reading the paragraph before it about the different syndromes, I thought I was going to be reading more of the same and was surprised to see that there was talk of schizophrenia and Parkinson’s.  I’m not exactly sure what you should change it to if you do decide to do that, but it might make it clearer to make it a big more specific to your paragraph.  And the Olympic screening was very interesting! Were there specific cases of this that you could link in? I found myself wanting to read more into the situation and learn about athletes that were found to be SRY-positive.  If you could add more about that, I think it would be a nice addition.  So, overall, your article is very well done. I thought you did a really good job getting the major points and you referenced well.  I think you could use a few more pictures if you can – maybe in the last part with the different disease or the athletes (maybe a picture of an athlete who couldn’t compete if that’s possible to find?) – and finding up some flow issues.  Once you fix the flow, I think your article will be superb and it clearly shows you’ve put a lot of work into it already. Great job! T1d7 (talk) 17:55, 22 November 2013 (UTC)
thread_title:  Peer Article Review 

message: Olympic testing: added additional information about the testing currently used for gender identification and more specifics of athletes that were found to have the SRY gene (it seems specific names of athletes are not available for privacy reasons) BASEDbio (talk) 22:19, 6 December 2013 (UTC)
thread_title:  Response to Peer Reviews 

message: Hello fellow Wikipedians,
I have just added archive links to  on Testis determining factor. Please take a moment to review my edit. If necessary, add  after the link to keep me from modifying it. Alternatively, you can add  to keep me off the page altogether. I made the following changes:
*Added archive https://web.archive.org/20100126090814/http://www.123hjemmeside.dk:80/Facius/7031466 to http://www.123hjemmeside.dk/Facius/7031466
When you have finished reviewing my changes, please set the checked parameter below to true to let others know.
Cheers.—cyberbot IITalk to my owner:Online 07:38, 10 January 2016 (UTC)
thread_title:  External links modified 

message: Hey, I've chosen this article to edit for a biology class. It looks like it's been a while since anyone edited the talk page, but I thought I would ask if there are any edits the community thinks need to be made? Otherwise, sources permitting, I would like to edit the sections about evolution, the role in the nucleus, and the role in other diseases. Thanks for any advice! Cluffa (talk) 21:59, 27 February 2019 (UTC)
thread_title:  Classwork 

message: This Wiki page seems pretty thorough already, so adding new parts to sections would be what I would focus on. There has to be more information out there for the weaker sections. The Evolution of the SRY gene could be a part to focus on since there seems to be a lack of information. There is probably studies you could find if you searched for the evolution of it. That would be useful information to have. Continuing on things you could add to, the part near the end talking about ongoing research could use help. Maybe adding new research to that area to explain what is specifically being looked at could be good information.  — Preceding unsigned comment added by Oimoenm (talk • contribs) 20:13, 31 March 2019 (UTC)
thread_title:  Peer Review 

message: Overall the article is thorough and has enough pertinent information on the topic for people to gain a general understanding of the testis determining factor. Some things that may need to be touched up is linking some of the forms of analysis like microarray and chromatin immunoprecipitation to the appropriate wiki pages. The last paragraph may need some more added to it if there is more out there, and the second sentence needs a citation attached to it I think. Those fixes are small, and I think otherwise the article is solid. It has a nice lead section and has most of the appropriate links and citations as well. Some more information could be added to the ongoing research section or possibly the role in other diseases, but I like the section on the olympic screening as it remained objective and gave a nice overview of the history of a semi-controversial topic. Mindiolam (talk) 19:28, 7 April 2019 (UTC)
thread_title:  Peer Review 

message: This article overall does a well job of explaining what the testis determining factor (TDF) is. It goes into detail on how the process of TDF works to create testis through transcription and upregulation. Throughout this article, include some more information and sources about the evolution of the SRY gene because I would personally like to know more about it. Although the section about the SRY evolution is not too long, the information you do have is useful but I think there could be some more. It's really interesting to know that the SRY gene has been shown to have come from the original Y chromosome in humans and that it is only one of four genes on the Y chromosome. For this study, using mice was plausible since they are mammals and easy subjects to study and they reproduce easily. Overall this article has plenty of information but it could use a few edits. In the regulation section, include links to certain terms that may not be understood by the average person coming across this article. Also during the Action in the nucleus section include some links as well for terms such as nuclear localization, importin β, calmodulin, Amh gene, and prostaglandin D synthase (Ptgds) gene. In general, this article has a lot of useful information but could use some more in certain areas, especially after having links to certain terms. There is plenty of information to be understood so adding more sections that go into detail about these would be helpful. The lead section of the article is great and gives detailed information about how the testis determining factor works. There is a clear structure among this article but it could be improved by adding more sections. There is a balance between each section, it is broken down nicely into three sections with subsections under each. I believe that they are in the right order of importance when pertaining to the TDF. The content and the way it is written is in a neutral point of view. There is no overarching viewpoint over the other and the authors did a good job of keeping the paper neutral. The article does have plenty of reliable sources with useful information to further improve this article. Overall this article was an interesting read and I learned valuable information that will be useful throughout this class. Although there are some small grammar errors and not having links to certain terminology, this is a well written article. Vohraa (talk) 06:11, 10 April 2019 (UTC)
thread_title:  Aafreen's Peer Review