Talk:Human leukocyte antigen

message: Quote: "Historically, HLA genes were identified as a result of the ability to successfully transplant organs between HLA similar individuals." ... I suspect this sentence should read something like "Historically, HLA genes were identified as a result of rejection problems even transplanting between similar blood group individuals."  Could someone please confirm this change???  121.91.106.141 (talk) 10:25, 28 November 2010 (UTC)
thread_title:  Human Leukocyte Antigen 

message: I need to collect the blood sample of the recipient and the donor for HLA Typing and Cross Match and transport it to another city. Can somebody guide me on which medium to collect the samples in, whether anticoagulants are required or not and whether I need serum or whole blood? Thank You.[REMOVED]
Answer: For HLA typing it is recommend to have about 10 mls of blood. When I surveyed
HLA DQA1 and DQB1 typically 300 microliter to 1.5 millliliter per patient per test, so that safely per typing analysis one needs about 3.0 mls for DQ typing about the same for DR (as the test is considerably larger). The blood should be taken into a vacutainer (Light Blue, Sodium Citrate 3.2%) and is stable if kept at 4'C for a long period of time. 4'C can be obtain on slightly wet ice, or wet ice 0'C but freezing is not good becuase it lyses the cells. I have found that with older individuals more cells are required, so if you are over 50 plan on giving the entire amount of 10 mls. [BTW don't use heparin, green topped tubes, heparin interferes with DNA collection and PCR]
10/1/2006 - prd
I'm sitting in an HLA lab that receives yellow top tubes exclusively (they've never seen blue tops here) so call your HLA lab and check with them before you send your sample.  They're very nice people.  —Preceding unsigned comment added by 198.133.139.5 (talk) 13:30, 3 October 2007 (UTC)
replies: text: I use yellow top tubes also, the light blue tops and yellow top tubes contain different citrate solutions, Yellow top tubes are acid citrate dextrose (ACD). The dextrose keeps cells healthier longer but if you are immediately preparing cells or freezing them at -80'C it does not make a difference. The thing one never wants to do is to freeze blood with heparin. Heparin can loosen the heme from hemoglobin and if that makes its way to the nucleus of the WBCs, effectively, the DNA is ruined.PB666 yap 04:38, 27 July 2008 (UTC)
thread_title:  How to collect Blood Sample for HLA Typing? 

message: I think this article needs a new introduction, understandable to a general reader.  I didn't get a clear indication that HLAs are actually part of the human immune system until the section on HLA Functions where it describes the function of HLA in the process of helping the T-cells to identify non-self, infected, and tumor cells via surface antigens. It's sorta part of the immune system's IFF (Identification Friend or Foe) subsystem, as it were.  Yes, this is simplistic, but I think that many readers who aren't familiar with cellular biology might need an Issac Asimov type explanation, at least initially.  I already knew HLA's importance to the immune system functioning before reading this article, but others that come across the term elsewhere may be looking to find out what a HLA is, what it does, and it's importance to us.  Becksguy 14:14, 12 June 2007 (UTC)
replies: text: The article has been reorganized, the a small description of function has been made in the header, and the function subsection has been brought forward. Illustrations of HLA MHC Class I and II have been added.Pdeitiker 03:56, 7 July 2007 (UTC)
replies: text: If you ever need a good, reliable, understandable introduction, I would recommend Kimball's Biology Pages. Kimball is a retired Harvard biology professor, and in this site he put his textbook online and keeps updating it. Another good source of a good, reliable, understandable introduction is the Merck Manual Home Edition here. Unfortunately, some of the free online dictionaries aren't too good; they have generic definitions that are literally correct (usually) but don't help anyone understand the concept. I knew people who wrote those definitions, usually freelancers who got today's equivalent of $2 per definition. Nbauman (talk) 16:56, 12 January 2009 (UTC)
thread_title:  New Introduction 

message: Added a sentance abut MHC in the first paragraph, the first mention of MHC was not until the classification section, and I don't think the link between the two terms is clear in the article anyway. At the moment some parts of the article suggest that MHC are a type of HLA, while other sections appear to suggest that HLA are a type of MHC. (as far as I am aware the latter is correct), it is a little confusing if the reader hasn't studied MHC before.Philman132 (talk) 12:00, 26 April 2009 (UTC)
thread_title:  MHC 

message: In the "Major MHC class II and HLA-DR" part there is a statement: "4 β-chains (only 3 possible per person), encoded by HLA-DRB1, DRB3, DRB4, DRB5 loci"
Could the "only 3 possible per person" statement have a reference or an explanation?
193.6.152.93 (talk) 09:14, 15 February 2013 (UTC)
thread_title:  Classification 

message: Am I missing something? The percentages in that table don't match, if 68 corresponds to 3.4%, 145 cannot be 21.5%. 
If I recalculate the percentages for common and well documented alleles based on the rare and very rare percentages (common: 68*41.6/280=10.1%; well doc: 178*41.6/280=26.44%), the percentages add up to 100% (21.5+41.6+10.1+26.4=99.6). So where do the percentages for C and WD come from? Ssscienccce  (talk) 03:38, 14 September 2015 (UTC)
thread_title:  Table of HLA alleles in each prevalence category 

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