Talk:Western blot

message: Westerns are not only used in molecular biology ;-), perhaps "protein chemistry" may be more appropriate (from Hennef, Germany)
How is the word incubate being used here? What keeps the protein of interest from washing away? Why does binding with an antibody make the protein stick to the nitrocellulose membrane?
Western should be written like western, not Western because it does not come from a name like Southern blotting.
:The commericial instructions sitting on my desk say Western is correct. Ladlergo 20:16, 4 April 2006 (UTC)
thread_title: Old stuff 

message: The article says:
The membrane is "sticky" and binds proteins non-specifically
You may have previously seen proteins stuck to surfaces.....maybe blood stains or grass stains. The plastic membranes that are used for a Western blot hold onto the proteins tightly and you do not have to worry about the proteins washing off of the membrane during the antibody steps.
The antibodies have nothing to do with the target protein(s) staying attached to the membrane. The antibodies specifically bind to their target protein by a molecular lock-and-key type of interaction. The antibodies that are used for Western blots typically have a slow rate at which they detatch from their target protein, allowing them to stay attached through the various incubation and rinse steps.
also known as an immunoblot
I noticed that "Tissue preparation" is listed as the first step the immunoblot (western blot). It should be "Sample preparation". I've done hundreds of immunoblots, but only a few from tissue culture cells. As a molecular microbiologist, most of my samples are from yeasts, subcellular fractions of yeasts,  or their culture supernatants. There are a lot of other samples besides just 'tissue'  —Preceding unsigned comment added by Yeastpics (talk • contribs) 11:08, 30 August 2010 (UTC)
thread_title:  proteins sticking to the membrane 

message: For those confused by my edit summary it should read "that I think's most common", but some fool decided to put the apostrophe key next to the enter key! Joe D (t) 16:57, 10 January 2006 (UTC)
thread_title: er...

message: User Alan Au, seems to hate Molecular Station no matter if the pages have quite a lot of information.  I believe Alan Au has no idea about bioinformatics or any other molecular biology information. He believes RNA Bioinformatics is not "accepted by the scientific community" and removed my contributions to that page. See Bioinformatics Talk.
Compare these pages:
Western Blot
Western Blotting.  They both have a lot of information, although one was deleted. See discussion at Alan Au's Editing. 
Wikipedia will never be a good resource for scientists, researchers and the general public as we do not have highly educated (Ph.D's, M.Sc's) individuals contributing as many of their contributions are being deleted.
I am a PdD who ran western blots a year or two after they were first described, and i have contributed to this article..I don't have the energy to compeltly re write it (needed) esp since my technically correct rewrites get trashed by people who write things that are just plain wrong.
Having said all that, as  sicentist who does biotechnology, I , and to my perosnal knowledge, other scientists, often rely on wkipedia as a first line of info.Cinnamon colbert (talk) 22:06, 22 February 2009 (UTC)
thread_title:  Alan Au Editing 

message: Just a quick nitpick... chemiluminescence and fluorecence are not the same thing, although they do have similar photophysical mechanisms. Fluorescence refers to the excitation of a molecule to an excited state, and the subsequent decay by radiative emission from that excited state. Chemiluminescence refers to the situation where a molecule is formed in an excited electronic state as the product of a chemical reaction, and subsequently decays by radiative emission to the ground state. There are a couple of instances in the article where chemiluminescence and fluorescence are mentioned as though they are interchangeable... not a big deal, just thought I'd mention it. -Oli 203.63.0.143 23:36, 11 June 2006 (UTC)
replies: text: or, leaving aside the technical mumbo jumbo...in fluorescence, a molecule absorbs a particle of light (a photon) and then emits a second photon. The energy of the 1st photon is greater then the energy of the 2nd photon.
In chemiluminescence, a chemical change releases energy that is "stored" in a molecule, and the released energy appears as a photon. This is highly unusual, as most chemical changes that release energy (eg, burning of gasoline) result in the release of heat.  —Preceding unsigned comment added by Cinnamon colbert (talk • contribs) 13:34, 25 February 2009 (UTC)
thread_title:  Chemiluminescence != fluorescence 

message: From the Southern blot article: "As the technique was eponymously named, Southern blot should be capitalised, whereas northern and western blots should not."
--Gycklaren 10:48, 27 May 2007 (UTC)
replies: text: It was capital W when I was a student in the 80s. 2A01:CB0C:CD:D800:60E4:E8AC:CCF6:6E79 (talk) 14:34, 19 January 2021 (UTC)
thread_title:  Consistency? 

message: The picture on the right seems to be missing? --David Munch 13:20, 17 June 2007 (UTC)
thread_title:  Article picture 

message: Should it be "western blot" or "Western blot"?  Strictly speaking "western blot" does not seem to be a proper noun.  However, the CDC journal of style (http://www.cdc.gov/ncidod/EID/style_guide_p2.htm) supports using a capital W in "western." I've seen it written both ways in both scientific articles and prose.  Science, the premiere American science magazine, uses a capital W and Nature, the premiere British one, uses lowercase.  Thoughts?12.144.50.194 (talk) 17:55, 21 February 2008 (UTC)
replies: text: I checked a the style guides for submitting to various science journals and the CDC website and most capitalize Western blot. The World Health Organization, however, does not capitalize eastern, northern or western blot, only Southern blot (named after the biologist). This article uses a mixture of cases so I changed them all to upper case - feel free to change to the lower case as long as they are consistent. Dr d12 (talk) 20:07, 9 May 2008 (UTC)

text: I'd say that if Burnette spelled it that way we should go with it, because that's probably the most definitive answer we will get. However, didn't he capitalise the entire term, i.e. Western Blot instead of Western blot? Unfortunately I have no access to the paper ATM, but maybe someone can check this: . -- Shinryuu (talk) 21:38, 11 October 2011 (UTC)
replies: text: In the paper 'Blot' is only capitalized in the title (along with 'Proteins', etc., as is usual for titles). In the remainder of the article it's 'blot' and 'blotting'. 129.67.74.57 (talk) 10:53, 12 October 2011 (UTC)
thread_title: Capitalization

message: as a PhD who was one of the first people in the world to actually do a western with my own two hands, shortly after Towbin et al, this article is getting worse with time; it used to be better !!!!!
(and I do know what i am talking about; I know that Ponceau S is now the preferred post blot protein stain, vs the commercial product from pierce (anthranavally et al) or direct blue/black or eosin or copper based stains; I know that BCIP/NBT fades on a dry pvdf blot, but that DAB is supposed to be toxic, ...)
the fancy graphics are just bad
I know, it is wiki, don't complain, fix it, but I get tired of having to re re re re correct stupid errors put in by others.  — Preceding unsigned comment added by 50.195.10.169 (talk) 18:03, 21 March 2013 (UTC)
replies: text: I seriously doubt you're an expert on the subject. I think you're a troll who has nothing better to do than complain about wikipedia articles. If you are indeed an expert, make an account and contribute, instead of complaining. Higgyrun3 (talk) 00:03, 27 March 2013 (UTC)
thread_title: getting worse with time

message: Does the term RIBA have anything to do with this topic?
I have edited the Royal Institute of British Architects article to change:
to
But the Riba (disambiguation) page does not list this page.
If a user is likely to search for this topic using "RIBA", then please add this article to Riba (disambiguation). Verbcatcher (talk) 01:54, 16 February 2014 (UTC)
thread_title: RIBA

message: --User:Ceyockey (talk to me) 13:43, 14 September 2014 (UTC)
thread_title:  Nature Methods paper to consider for inclusion 

message: It seems that the article claims fluorescence is more sensitive than chemiluminescence but then claims the opposite in a later paragraph. Not sure which is correct, but it's something to look out for.  — Preceding unsigned comment added by 143.215.227.205 (talk) 18:22, 31 December 2014 (UTC)
thread_title: Contradiction

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thread_title:  External links modified 

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thread_title:  External links modified 

message: Hello All,
Would it be possible for me to introduce a technology that automates Western blots (http://www.proteinsimple.com/simple_western_overview.html)? It's been widely accepted in publications, journals, academia and industry folks. I want to show how Westerns can be performed via capillary electrophoresis. 
Thank You,
Steven — Preceding unsigned comment added by Steven.le57 (talk • contribs) 17:10, 9 May 2019 (UTC)
thread_title:  Automated Western blot technology introduction and overview  

message: I'm no expert, but this looks relevant to include?
Chinese ministry investigates duplications in papers by university president
Prevalence of Inappropriate Image Duplication in Biomedical Research Publications
— Preceding unsigned comment added by ChrisLambertUK (talk • contribs) 22:31, 27 November 2019 (UTC)
thread_title: Controversy?

message: I am a Molecular Biology master student and, this is the article that assigned to me under the Recent Developments in Biotechnology Course. Further details are available on the course page. 
Student editor: Sumydrmz
Sumydrmz (talk) 18:43, 11 December 2022 (UTC)
replies: text: The course page link is: outreachdashboard.wmflabs.org/courses/Uskudar_University,_Istanbul,_Turkiye/MLC501_Recent_Developments_in_Biotechnology_2022-2023_Fall_(2022-2023)/home Sumydrmz (talk) 08:12, 20 December 2022 (UTC)
thread_title:  Wiki Education- Course Assignment