Talk:Immunohistochemistry

message: Ok it decribe the procces but what is measured? seen? and what is its goal?--Scubafish 18:46, 12 June 2006 (UTC)
thread_title: basics

message: The article are almost exactly the same. Need to be merged, both articles would benefit.--DO11.10 18:48, 16 March 2007 (UTC)
replies: text: Agree I didn't notice that immunohistochemical staining existed as a page. It is redundant and not as thorough as immunohistochemistry. Merge ASAP Dr Aaron 06:22, 17 March 2007 (UTC)
thread_title:  Proposed_Merge_with_Immunohistochemical staining 

message: From a technical perspective, the two procedures are virtually identical except for sample preparation. Permeabilization is also more critical in ICC vs. IHC, but by and large, the two only differ in minor details. At present, the immunocytochemistry article needs a lot of work and I believe merging would benefit both articles in reducing repetition. Immunocytochemistry should take the form of a section in the Immunohistochemistry article. This section could highlight some of the key differences between the two. 142.103.207.10 19:32, 22 August 2007 (UTC)
replies: text: Good points - support merge of ICC into IHC. -- MarcoTolo 19:42, 22 August 2007 (UTC)

text: I have just realized that an immunostaining page already exists and links to IHC as well as several other techniques. As such, I suggest that ICC be linked to in that page as well and this article be considered a stub until someone chooses to expand it. What is the benefit of putting them together when a summary page already exists? - Dante Marx 20:26, 11 September 2007 (UTC)
replies: text: I think that you should merge ICC - it is essentially the same thing (cells vs tissues) and in practical terms the two are often used interchangeably. Dr Aaron (talk) 13:23, 13 March 2008 (UTC)

text: Since this discussion is already well along, I've taken steps to bring all those interested in this most recent question to this page: there are now notes on the talk:immunostaining and talk:immunocytochemistry pages that will bring people over here before they start redundant discussions. — eitch 17:04, 18 April 2008 (UTC)
thread_title:  Proposed Merge from Immunocytochemistry 

message: This article was automatically assessed because at least one WikiProject had rated the article as start, and the rating on other projects was brought up to start class. BetacommandBot 16:28, 10 November 2007 (UTC)
thread_title:  WikiProject class rating

message: I tagged one sentence for implying IHC can be used to "diagnose and treat cancer." 
Also, I feel as though the Immunotherapy section seems out of place and should be removed. Any comments?
AkashAD (talk) 15:32, 24 December 2007 (UTC)
replies: text: I agree that the immunotherapy section has nothing to do with IHC. It should be removed. Dr Aaron (talk) 13:23, 13 March 2008 (UTC)
replies: text: I disagree.  Give me a little time, and I'll track down some good references on the use of immunohistochemistry in directing therapy.  The first use was estrogen and progesterone receptor positivity for treatment with tamoxifen.  Then Her-2/neu for Herceptin came.  There are some other now -- c-kit for Gleevec, and others.  I can have the section together in a couple of days, tops.  As tamoxifen and Gleevec are not monoclonal antibodies I will change the section title as well.   Novangelis (talk) 13:55, 13 March 2008 (UTC)
thread_title:  Immunotherapy 

message: From the section Direct and indirect IHC: "There are two strategies used for the immmunohistochemical detection of antigens in tissue, the direct method and the indirect method. In both cases, the tissue is treated to rupture the membranes, usually by using a kind of detergent such as Triton X-100."
This quote highlights the confusion some people have between immunohistochemistry and immunocytochemistry. Permeabilization is more important in ICC because the samples are whole cells. In IHC, the sample is a tissue section, which has been "permeabilized" by having a microtome blade go through it. Detergents are used in IHC to reduce surface tension, not for permeabilization.
142.103.207.10 (talk) 22:56, 14 May 2008 (UTC)
thread_title:  IHC and ICC confusion 

message: Many articles mention "Immunohistochemical profile"s, and that term now redirects here but there is no description here. What is an IHC profile, eg. is it a list of some set of cell surface antigens with +/- or quantitative values for each ? ( Rosehip neuron mentions "immunohistochemical profile (GAD1+CCK+, CNR1–SST–CALB2–PVALB–)" ) Are there any standards or are they different for each application or disease ? - Rod57 (talk) 11:01, 12 September 2018 (UTC)
thread_title:  Immunohistochemical profile could be mentioned