Talk:Lambda phage

message: The discussion seems to be overly technical in places. Also, is point 7 under Lifestyle backwards? It seems to say it will lyse unstressed cells and integrate into stressed cells. 24.18.247.19 22:50, 7 January 2006 (UTC)
replies: text: *Please see this PDF file for information on this (Ilyas is right). I wish I could use some of the images, but they are undoubtedly non-free. --N Shar 01:22, 27 October 2006 (UTC)
replies: text: Done. I'm not sure I did a good job, so if anyone wants to look at it... --N Shar 01:17, 27 October 2006 (UTC)
thread_title: Lytic or Lysogenic?

message: The statement is correct but confusing. When the phage first encounters the cell and all its genes are expressed, the activity of cII depends on the amount of nutrients available, because this controls protease activity. If nutrients are abundant, the proteases actively cleave cII and cause a lytic cycle (counterproductive, perhaps, but evolution does not result in the best possible organism). If nutrients are sparse, cII is not cleaved and the lysogenic cycle begins.
Later, however, when the lysogen is "stressed" by UV light, cI is cleaved by the bacterial protein RecA and the lytic cycle is induced. This is the reverse of what happens initially.
Perhaps it would be best to remove all occurances of the word "stressed" to clarify this. I may do this later when I can log in. --67.125.30.232 17:58, 26 October 2006 (UTC)
replies: text: Done. I'm not sure I did a good job, so if anyone wants to look at it... --N Shar 01:17, 27 October 2006 (UTC)
thread_title:  Re: point 7 (Lifestyle) 

message: Why was this changed? My source was my uni notes but I can't find it anywhere else... If anyone can find a number with a reference that would be nice :).
It seems that there are 48,502 base pairs in the circular genome... actually in the phage particle head are 48,490 base pairs of dsDNA and 12 bases of ssDNA at either end of the molecule. These numbers could instead be 48,514 and 48,502... I'm trying to find out for sure.--Gorton k (talk) 15:56, 17 February 2008 (UTC)
thread_title: Base-pairs of Genome

message: Personally, some figures (especially for the CI/Cro lysogenic regulation section) will really help with understanding for those new to the material. Does anyone know of any good ones [I have the diagram from "Molecular genetics of Bacteria" here]? Jimhsu77479 (talk) 05:13, 18 May 2009 (UTC)
thread_title:  Figures 

message: Can someone revise these sentences: "The phage genes expressed in this dormant state code for proteins that repress expression of other phage genes [what other phage genes? Those natural to the bacterium? What role do they play?]. These proteins are broken down when the host cell is under stress, resulting in the expression of the repressed phage genes"? For the last sentence, wouldn't it be better: "The proteins expressed by the inhibitory phage genes [...] resulting in the expression of the repressed ones"? Wisapi (talk) 20:49, 19 September 2010 (UTC)
thread_title:  Intruduction revision needed 

message: I notice that there is no mention of cro binding to PL and inhibiting protein N production. However I've not been able to figure out the physiological reason for this binding. Rhodydog (talk) 22:28, 10 May 2013 (UTC)
thread_title:  Cro binding to PL 

message: I am a physician, not a biologist.  I got here from studying wikipedia on the Irish potato famine and it's causes, both social and biological.  I wondered, and address those who are more veteran at things wikipedia.  It occurs to me that this article needs a section, perhaps short, geared to an intelligent layman audience, on why this article is rightly classified as high importance.  How is that done without going "off topic?"  — Preceding unsigned comment added by Purebread (talk • contribs) 16:45, 17 March 2016 (UTC)
replies: text: If you want to demonstrate importance, I would suggest an additional paragraph in the lede indicating two things that make lambda so notable - it is universally used in molecular biology for the construction and screening of genomic and cDNA libraries, prior to PCR the only way to clone genes, while likewise it served for about three decades as the prototypical example of a complex multi-gene regulatory system and was used to teach these coordinated processes to virtually all graduate students in biomedical fields. Without these dual bases for notability, it would be just another virus.  69.166.47.99 (talk) 22:07, 22 April 2016 (UTC)
thread_title:  Suggest section 

message: The article should mention how many genes lambda has. 2A02:8388:1641:4980:91F8:9D5D:211B:93E3 (talk) 02:18, 8 April 2023 (UTC)
thread_title:  Number of genes