Talk:Oligonucleotide synthesis

message: Phoshoramidite entry is similar to this but made indipendently, I think.
It should be fixed as it contains some extra stuff but lacks other stuff and does not link here.  —Preceding unsigned comment added by Squidonius (talk • contribs) 16:55, 25 October 2007 (UTC)
thread_title: similarity

message: There is a mistake in the drawing; an extraneous oxygen is present between the cytosine N1 and the ribose ring.  —Preceding unsigned comment added by 140.247.246.12 (talk) 19:06, 9 April 2008 (UTC)
thread_title: drawning

message: Has there been any progress in the length of sequence that can be synthesized?  The article says:
The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized.
However, the reference is from 1992. Any progress since then?
pgr94 (talk) 10:34, 25 June 2011 (UTC)
replies: text: Thanks, it does indeed. pgr94 (talk) 13:22, 2 October 2011 (UTC)
thread_title:  Sequence length 

message: Hello. In this article I read: "The two most often used solid-phase materials are Controlled Pore Glass (CPG) and macroporous polystyrene (MPPS).[55]"
I checked the resource [55] and I havn't found anything about polysterene, it's just all about CPG.
I need more informations about macroporous polystyrene (non-swellable polystyrene) for solid-phase oligonucleotide synthesis. I haven't even found it commercially available. I would appreciate some more information - even where to buy it or a CAS No - Thanks.  — Preceding unsigned comment added by 134.100.29.35 (talk) 09:33, 18 September 2011 (UTC)
thread_title:  Solid Support Material 

message: In Nucleoside_phosphoramidite I read that the phosphoramidite reacts with water to a H-phosphonate diester (see picture: [here]).
"When water is served as a nucleophile, the product is an H-phosphonate diester as shown in Scheme above. Due to the presence of residual water in solvents and reagents, the formation of the latter compound is the most common complication in the preparative use of phosphoramidites, particularly in oligonucleotide synthesis."
I have synthesized a modified phoshoramidite (as a crude product!) and I want to use this crude product for the coupling step without any further purification. In case that I have some H-phosphonate byproducts, will they also be coupled to CPG? Are H-phosphonates even soluble in acetonitrile or dichloromethane?
What about hydrolized Bis-N,N’-diisopropylamino-(2-cyanoethyl)-phosphoramidite: will this compound also couple to CPG? --80.171.177.86 (talk) 14:49, 3 December 2011 (UTC)
replies: text: Could you move your discussion to your talk pages? Thanks. Carstensen (talk) 16:30, 13 December 2011 (UTC)
thread_title: Coupling Step: Influence of water

message: Why is the basicity of the exocyclic amino group of guanosine low that it doesnt need to be protected (Source?)? Compared to Adenine and Cytosine the pKA of Guanine is the highest with 12.3... Thet619 (talk) 23:29, 13 January 2013 (UTC)
thread_title:  Basicity of exocyclic amino group 

message: I noticed that you deemed this a Good Article, but it seems far too technical to me. Do you have an opinion on this? Exercisephys (talk) 18:11, 14 March 2014 (UTC)
replies: text: Hey Exercisephys, it's been several years since I evaluated this article, and unfortunately I must admit that I am not familiar at all with Good Article guidelines anymore. I'd value any additional input! -download ׀ talk 03:44, 15 March 2014 (UTC)
thread_title:  Too Technical? 

message: Hello fellow Wikipedians,
I have just added archive links to  on Oligonucleotide synthesis. Please take a moment to review my edit. If necessary, add  after the link to keep me from modifying it. Alternatively, you can add  to keep me off the page altogether. I made the following changes:
*Added archive https://web.archive.org/20111110054741/http://www.azcobiotech.com:80/instruments/OligoMaster.php to http://www.azcobiotech.com/instruments/OligoMaster.php
*Added archive https://web.archive.org/20110915101336/http://www.azcobiotech.com/instruments/NewSynthesizers.php to http://www.azcobiotech.com/instruments/NewSynthesizers.php
*Added archive https://web.archive.org/20121103005405/http://www.biosset.com:80/products/all to http://www.biosset.com/products/all
*Added archive https://web.archive.org/20110807171508/http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&ParentId=696378 to http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&ParentId=696378
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thread_title:  External links modified 

message: Hello fellow Wikipedians,
I have just added archive links to  on Oligonucleotide synthesis. Please take a moment to review my edit. If necessary, add  after the link to keep me from modifying it. Alternatively, you can add  to keep me off the page altogether. I made the following changes:
*Added archive https://web.archive.org/20110930100234/http://www.bioautomation.com/Synthesizers-MerMade%20384.html to http://www.bioautomation.com/Synthesizers-MerMade%20384.html
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thread_title:  External links modified