Talk:Nucleic acid thermodynamics

message: — Preceding unsigned comment added by Super randome user (talk • contribs) 09:52, 10 April 2018 (UTC)

message: Hej guys, I have some questions marks in my head about this section of the article. Especially about this sentence:
"[A], [B], and [AB] will be equal, and equal to half the initial concentration of double-stranded nucleic acid, [AB]initial."
and then you conclude that
T_m = -\frac{\Delta G^\circ}{R\ln\frac{[AB]_{initial}}{2}}.
But if [A]=[B]=[AB] then \ln{\frac{[A][B]}{[AB]}} = 0 and nothing else. 
The other question mark is about the units of the equilibrium constant:
K=\frac{[A][B]}{[AB]}
If [A], etc. is in mol/l then K has the unit mol/l   and hence 
K=ln\frac{[A][B]}{[AB]}
does not make sens.  
Super randome user (talk) 09:56, 10 April 2018 (UTC)
thread_title:  Derivation of Melting Temperature Wrong

message: The definition cited by this Article (SantaLucia1998) assumes a two-state model of DNA-hybridization (ds vs ss). This model is the basis for the nearest-neighbor model of SantaLucia, but does not completely reflect the nature of hybridization. There exist intermediate states of hybridization where only a partial hybridization between two strands took place (also called "hybridization cores"). Usually, the definition only includes a statement about the single-stranded strands and does not comment on the state of the remaining DNA. I changed it in the paragraph but I did not find a proper source for this.  — Preceding unsigned comment added by 192.124.248.157 (talk) 14:08, 14 January 2013 (UTC)
thread_title: Incorrect (?) Definition Melting Temperature

message: I know this section is small, but the DNA section does not actually talk about DNA melting in terms of why it happens. So perhaps this should be placed within DNA. I tried looking at Wikipedia what was the point of DNA melting but there was nothing Tourskin 01:52, 4 February 2007 (UTC)
thread_title: I

message: I feel the information from melting temperature can go in here as a subsection. In addition, I feel the article title "melting temperature" is too vague to only be refering to the DNA phenomenon. -Madeleine 01:13, 23 March 2007 (UTC)
replies: text: I agree; the name "melting temperature" is innapropriately vague, and "DNA melting" makes a far better title. In terms of content the two articles overlap and would be more approproiate as one. - Zephyris Talk 09:21, 4 April 2007 (UTC)
replies: text: Merger has been done. -Madeleine 17:52, 6 April 2007 (UTC)

text:  I might get use to people deleting my info if only they gave proper reason - so why was the info regarding the use of DNA melting deleted? The composition of the nucleotides determines the melting temperature. Well whoopee doo for finding that one out but doesn't that mean that mutations (which are weaker in bonding due to their abnormal positioning and hence bonding) will melt easier? Tourskin 21:47, 12 May 2007 (UTC)
replies: text:  I removed it because it was wrong and unreferenced. Mutations do not have "weird structures" -- a point mutation might start with a single nucleotide mismatch, but those are rapidly detected and repaired to form a stable bonded double-helix structure, and subsequent rounds of DNA replication match each base with its complement. Perhaps your wording was wrong, perhaps you were thinking of an obscure related technology that I have heard of, but you didn't give a reference and I'm not going to help you with this, because you're being rude. I find it disturbing that you apparently made this scientific page on wikipedia claiming a statement of fact that was in merely based on your own speculations. Madeleine 22:14, 12 May 2007 (UTC)
thread_title:  Proposal to merge 

message: I think this is the more general term, since it's used for chemical denaturation as well as heat, although the article still should refer to "melting" of course and there will be redirect. Any comments / objections?  Madeleine 20:16, 20 May 2007 (UTC)
replies: text: I have no objections. Tourskin 22:48, 20 May 2007 (UTC)

text: DNA denaturation is the correct term, is it not? In terms of accepted nomenclature, "melting" is an inaccurate term. I remember being taught as an undergraduate (many moons ago now) that the concept of "melting temperature" is just a common misconception of the abbreviation "TM"; the actual term being "median temperature". Argh 01 (talk) 09:20, 12 May 2011 (UTC)
thread_title:  Move page to "DNA denaturation" 

message: The 'separion' inserted long ago . Insertad with 'urea' addition. In fact urea act during  dissociation. Also urea buffer is used to DNA separation  . (Separation is error here separation of DNA in urea buffer on chromatography columns...) Dissociation is more correct term. Xook1kai Choa6aur (talk) 04:19, 31 August 2009 (UTC)
thread_title:  dissociation or separation ?

message: Could someone add a typical melting temperature or a range (maybe for some typicals strands).
77.11.52.78 (talk) 17:40, 12 January 2010 (UTC)
replies: text: The melting temperature depends on the base composition as well as the length of the DNA sequence. So there is no fixed melting temperature or a narrow melting temperature range.  — Preceding unsigned comment added by 192.124.248.157 (talk) 14:36, 14 January 2013 (UTC)
thread_title:  Typical melting temperature 

message: This article, now titled Nucleic acid thermodynamics, was the result of a merge between DNA denaturation (the former title of this article), Nucleic acid hybridization, and Annealing (biology).  I'd like to note that I made an error in one of the edit summaries: the second edit after the page move, at 17:07, 9 April 2010, was actually the merge from "Annealing (biology)" and not "Nucleic acid hybridization". Antony-22 (talk) 21:02, 13 April 2010 (UTC)
thread_title:  Merge 

message: The scope Nearest-neighbor method has been extended beyond melting temperature context. The extension include the possibilities of non-Watson-Crick pairings (mismatches) with appropriate reference.
FysBio (talk) 15:06, 27 March 2013 (UTC)
thread_title:  Extending the scope of Nearest-neighbor model