Talk:Tris

message: the MW of tris is 121.14 g/mole, the MW of tris-HCl is 157.59
http://www.jtbaker.com/msds/englishhtml/t7112.htm 129.3.17.129 (talk) 00:15, 22 June 2010 (UTC)
thread_title: Molecular weight

message: The Solubility listed (220g/100mL) is not correct.  
This would make an 18.16M solution of Tris, which is definitely insoluble at RT (I just tried to make 5M @25C, heated it, sonicated it, stirred it overnight, really banged on it, and it was a no go.  I was however able to make 2.5M Tris without much difficulty [at pH 8]).  I think the solubility is somewhere closer to ~4M (somewhere in the neighborhood of 50g/100mL) but I can't seem to find information about saturated solutions of Tris in any of the CRC handbooks.  Can someone more knowledgeable please correct this information, or baring that remove the solubility?  —Preceding unsigned comment added by 137.131.130.41 (talk) 17:27, 11 August 2008 (UTC)
thread_title: Solubility

message: If anyone ever wants to construct an infobox, here are the particulars. savidan(talk) (e@) 09:02, 9 April 2006 (UTC)
replies: text: Some of the analysis information you have here is only going to be specific to that lot. They aren't innate properties of tris itself. Benzene 15:10, 6 June 2006 (UTC)
thread_title:  Infobox 

message: The two alternative systematic names:  2-amino-2-hydroxymethyl-1,3-propanediol or 2-nitro-2-hydroxymethyl-1,3-propanediol describe quite different compounds.  I would suggest that the latter (2-nitro) be deleted.
Dr Thermo 15:23, 26 October 2007 (UTC)
thread_title: Systematic name

message: The upper and lower case of Tris/tris is not consistent. What is the correct case?  —Preceding unsigned comment added by 137.248.208.240 (talk) 07:17, 13 October 2010 (UTC)
thread_title:  Upper or lower case? 

message: It appears to be vandalized, I lack the technical skills to revert this to the original. 94.209.235.75 (talk) 16:01, 18 December 2010 (UTC)
replies: text: .  Thanks for catching the error and reporting it here.  -- Ed (Edgar181) 18:01, 18 December 2010 (UTC)
thread_title:  Reference 6 

message: Tris is by far the most popular buffer for molecular biology and biochemistry (for cell biology it is phosphate) so I think it would be fair to highlight some of its drawbacks.
First of all a buffer with a pka above 8 is a poor choice for buffering at pH 7. Most people forget that. Additionally, Tris pka is much more temperature and concentration dependent than other buffers (and if the pka of the buffer changes the pH of the solution changes). Here's a link with some examples of changes in pH with change in temperature http://promega.wordpress.com/2011/02/25/what-makes-a-good-buffer/ 
And it's neutral at buffering pH so it is much more likely to transverse membranes. And that neutral primary amine is a good nucleophile as well. It is sterically hindered so less reactive than you would otherwise expect but it is a liability.
And while Tris is cheap, so is MOPS and HEPES (which on the other hand decomposes when autoclaved).
Structurebiologist (talk) 20:22, 2 July 2013 (UTC)
thread_title:  Criticism / drawback 

message: Substituted at 09:16, 30 April 2016 (UTC)
thread_title: Assessment comment

message: I removed references to Tris being one of Good's buffers from the article. Tris is not one of the 20 Good's buffers. Good's original 1966 paper used Tris as an example of a "bad" buffer in search for alternatives with better characteristics: "... some very inappropriate buffers have been used in biological research. These buffers have been bad because of inefficacy, undesired reactivity, or toxicity. '...' Tris(hydroxymethyl)aminomethane (Tris) has poor buffering capacity below pH 7.5. Moreover it is a primary aliphatic amine of considerable reactivity and consequently it is often inhibitory" (Quote from https://doi.org/10.1021/bi00866a011). This has also been emphasised in other articles, e.g. https://www.promega.de/en/resources/guides/lab-equipment-and-supplies/buffers-for-biochemical-reactions/; https://doi.org/10.1021/acs.biochem.1c00200 and is also mentioned in the Good's buffer Wikipedia article (https://en.wikipedia.org/wiki/Good%27s_buffers). 128.176.164.101 (talk) 12:52, 5 April 2024 (UTC)
thread_title:  Tris is not a Good's buffer