Talk:Enzyme/Archive 1

message: I've read the discussion below and I really feel that the article must at least mention ribozymes and link to a more specific article.
The kinetics section needs some work.  The Briggs-Haldane equation isn't even mentioned, and the use of double recipricol plots is not very common in research, except possibly as a quick test but it is so easy to fit the data properly now that I don't know anyone who uses them.
No mention of transient kinetics?
Any thoughts/objections?
Hichris 21:57, 30 November 2005 (UTC)
thread_title: As an enzymologist, a few notes:

message: These are a list of enzymes in the Wikipedia:Orphaned_Articles list they could certainly do with being properly catagorized and adopted on a page or two.  I know absolutely nothing about the subject (perhaps I should read the article) that is why I am dumping them here in the hope that one of you clever bods know what to do.
Phosphoglucomutase, Protein disulfide isomerase, Uridine monophosphate synthetase, ATCase, DNase I, DnaB helicase, Enoyl CoA isomerase, Galactokinase, Glucose Oxidase Enzyme
:Some of those are DNA enzymes which deserve to be in the DNA article.  Or maybe an article describing the proteins involved in DNA replication.  I haven't decided yet.  I'll give it a look.  --G3pro 13:30, 22 Sep 2004 (UTC)
replies: text: The enzyme that is most common in detergents is the protease Thermolysin, I belive it comes from a Bacillus sp. but don't hold me to that. look it up, good luck! Adenosine | Talk 05:55, 2 August 2006 (UTC)
replies: text: UPDATE! (Shamelessly ripped from thefreedictionary.com) Ther`mo`ly´sin n.1.(Biochem.) a proteolytic enzyme obtained from the bacterium Bacillus thermoproteolyticus, which hydrolyses the N-terminal amide bonds of hydrophobic amino acid residues in proteins. It is used in studies of protein structure. It has a molecular weight of about 37,500 and contains zinc and calcium in its active configuration. Adenosine | Talk 06:08, 2 August 2006 (UTC)
replies: text: We should add this somewhere on wikipedia Adenosine | Talk 06:08, 2 August 2006 (UTC)
thread_title:  Lost enzymes 

message: Somebody has defaced the enzyme page somewhat
* Thanks for bringing this to our attention. See also Wikipedia:Vandalism in progress. -Fennec (はさばくのきつね) 21:50, 18 Oct 2004 (UTC)
thread_title:  Defaced 

message: Why is there a section on protein purification here?  It is basically content free and irrelevant IMO.
Secondly, why is there a section on the quantum mechanical model of enzymes?  There is no actual explanation of the model.  I don't know what people thought in 1972, but as things stand today, quantum mechanics is considered to be mostly irrelevant to enzymology, because the molecules involved are too large, with the possible exception of the hydride transfer.  
It would make more sense to have an overview of the lock and key model, the induced fit model, and then a comparison to contemporary views.  --Theyeti 04:40, 17 Nov 2004 (UTC)
thread_title: Some Suggestions

message: There are two different meanings for the greek root words listed. Someone more knowledgable than me, please address this. 128.61.70.49 06:06, 28 Apr 2005 (UTC)
thread_title: Origin of the term

message: the diagram http://en.wikipedia.org/wiki/Image:Activation2.JPG is wrong
the blue line is wrong, it does not join the red line at the start of the reaction, the peak should be in the middle.
:Well, time is used as the independent variable. -- Jerry Crimson Mann 10:37, 29 July 2005 (UTC)
thread_title:  wrong diagram 

message: A user removed the following, saying it was speculation.  Could someone verify it before it gets put back in?
: Evolution works against having the amino acids from an active region widely dispersed, instead tending to keep the amino acids involved in each active region compacted fairly closely together in the chain and conserved against mutation, separating these regions by long stretches of "spacer" amino acids where mutation is much less critical (although some mutations in these regions can also inactivate the product). It is because the precise structure of each region tends to be fairly critical to correct function, and the frequency of a mutation which would produce a nonfunctional active region is proportional to the length of the chain separating the amino acids involved. This has the additional effect of making each region act, relative to mutation, somewhat like an independent subunit which can be duplicated, deleted, moved, or "mixed-and-matched" with other such regions, generating new proteins to be tested for evolutionary success. This would seem to be a more efficient process in terms of the likelihood of successfully generating functional new enzymes than would having the functionally related amino acids of the active site widely dispersed throughout the amino acid chain, with random mutation occurring anywhere.
Dave (talk) 17:46, August 2, 2005 (UTC)
:The sequence of amino acids in a protein determines its three-dimensional shape, which in turn confers its function. Segments of the protein that are critical to its function resist evolutionary pressures because mutations of such segments are often lethal to the organism. These critical "active sites" tend to be conserved over time and so can be found in many organisms and proteins that have similar function. Analogously, functionally important segments of an organism's DNA tend to be conserved and to recur as common motifs. In this chapter, the author introduces algorithms for comparing DNA and protein sequences to reveal similar regions. Particular attention is given to the problem of searching a large database of catalogued sequences for regions similar to a newly determined sequence of unknown function.  Gzuckier 19:57, 2 August 2005 (UTC)
thread_title:  Removed section 

message: Shouldn't the comment at the lower equation at Enzyme#Thermodynamics say "in lungs - low CO2 concentration"? Otherwise there would be no clear reason why the reaction should be reversed there. — Pt (T) 08:35, 5 August 2005 (UTC)
thread_title:  CO<sub>2</sub> concentration and balance 

message: Does this section add much to the article, I don't find it very informative.  The Metabolic pathway article covers nearly all of this material.  The allosteric effector info could be added to a more concise kinetics section.  Any thoughts?
Hichris 19:03, 5 December 2005 (UTC)
replies: text: I am with you in this analysis. In my mind this article should be briefer and more an introduction to the topic with links to the more complete articles in enzyme kinetics and metabolic pathways.  It it a lot of work to consolidate the kinetics articles which is why i have been reluctant to start but it definitely needs attention in the future. David D. (Talk) 20:53, 5 December 2005 (UTC)
thread_title:  Metabolic pathways and allosteric enzymes 

message: The user 81.139.75.141 will stop vandalising this page or the IP address will be permanently blocked from Wikipedia and all of its sister projects.
:::—gorgan_almighty 12:26, 8 December 2005 (UTC)
thread_title:  Vandalism