Talk:Reverse transcription polymerase chain reaction

message: RT PCR is also short for real time PCR
Real time PCR is not a variation of reverse transcriptase PCR AFAIK...Its simply a PCR reaction where the components are tracked in real time... Jarwulf 01:34, 31 Mar 2005 (UTC)
thread_title: Untitled

message: RT-PCR is a very commonly used technique in molecular biology (or at least it's one i'm very familliar with), and I want to do a complete overhaul of this page. There are too many typos, too many misconceptions, and too many omissions of the most important parts. I just started today, but over the coming days, I want to make big changes. Feel free to discuss anything with me. Thanks. --Michael (talk) 18:13, 28 February 2008 (UTC)
thread_title:  I want to do a complete overhaul... 

message: Hi all.  A group of us will be adding new contents to this article over next several weeks.  Our plan is to organize the article into following seven sections:
* Introduction
* History 
* Principles
* Application
* Challenges 
* Protocol
* Publication Guidelines 
Your suggestions, comments, and edits are always welcomed and appreciated.  Kkotani1 (talk) 01:44, 14 November 2012 (UTC)
thread_title: Plans for contents addition

message: Hi, guys. Here's a final-week mini-review of your page. It is just a few random notes, that I jotted down while skimming the page, and is not intended as a comprehensive review.
* It would be nice if you could add some figures.  Please see some suggestions here, under "Figures and Images".
* "is often interchanged with":  do you mean "is often confused with"?
* Under "History", you have some content that seems to have nothing to do with history.  For example, the last paragraph of that section.  Perhaps this content could be moved to some other section(s) where it would fit better?
* Under "Principles" you have the same problem, in the paragraph beginning, "Despite its major advantages, RT-PCR is not without drawbacks."  Perhaps you could have a "pros and cons" subsection at the end of "Principles", and move these there -- it is just a thought, other solutions might be better.
* In general, your article is pretty good, and is among the best from this class that I've looked at so far.  You might look to an article GA-rated article like X-ray_crystallography for some ideas, if you want to restructure it a little bit during this last week.
* Under "Application", I think each of the paragraphs that have a bold inline-header, should really be a subsection.  That way, they'll show up in the table of contents.
Klortho (talk) 18:45, 9 December 2012 (UTC)
replies: text: Thanks for the suggestions! How do I go about making each paragraph that has a bold inline-header in the "Abbplication" section a subsection?MHN2785 (talk) 16:37, 12 December 2012 (UTC)
replies: text: Boghog already fixed that in this edit.  See Wikipedia:Layout#Headings_and_sections. Adrian J. Hunter(talk•contribs) 00:13, 13 December 2012 (UTC)
thread_title:  Final week mini-review 

message: Substituted at 04:14, 30 April 2016 (UTC)
thread_title: Assessment comment

message: rRT-PCR, real time RT-PCR. is used in  Coronavirus_disease_2019. rRT-PCR appears to be the contemporary (2020) usage and preferable to RRT-PCR. The mixed case, rRT-PCR, usage needs to be included in Nomenclature.
LarryLACa (talk) 21:08, 3 April 2020 (UTC)
replies: text: "... monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR)." certainly suggests rRT-PCR = qRT-PCR. Whereas the next sentence, "Combined RT-PCR and qPCR are routinely used ...", suggests rRT-PCR and qRT-PCR are distinct. The next paragraph remarks "Such use may be confusing, ... ." Well yah, with a little imagination and effort jargon can cause confusion. Conversely, a lucid and honest explanation makes a concept evident and prevents confusion. Regards, ... PeterEasthope (talk) 02:23, 11 April 2020 (UTC)
thread_title: rRT-PCR=qRT-PCR