Talk:Microscopy

message: Currently the sub-diffraction limit section is a mess, it is badly written, quickly changing and far more complex than most of the rest of this article; would it make sense to split it to a separate article? - Zephyris Talk 10:13, 27 July 2009 (UTC)
thread_title: Sub-diffraction limit techniques

message: Hi, 
the section on super-resolution fluorescence microscopy (i.e. techniques like STED, SIM, PALM, STORM, FPALM,...) is really outdated. That field has developed rapidly over the last two years. Maybe someone could provide an update.    —Preceding unsigned comment added by 134.76.215.201 (talk) 22:10, 20 November 2008 (UTC)
thread_title: STED, SIM, PALM, STORM, FPALM

message: I am doing a major overhaul of this page over the course of the next week or two, and its not finished yet! so please don't revert all of my changes because it seems a bit unstructured, it will get better :D Zephyris 21:12, 6 August 2006 (UTC)
replies: text: yes, in some respects. my feeling is that microscopy refers to any mechanism which 'directly' images a target, which does apply to some particle collider experiments. I think that some experiments, such as electron(?) scattering to reveal neutron/proton quark structures, would be appropriate here, and a short mention in the introduction and a short section (as for scanning probe microscopes) would be suitable. - Zephyris Talk 01:38, 18 November 2006 (UTC)
replies: text: I'd draw the line at what you can find in good sources.  If you find a book that refers to these things as microscopy, go for it. Dicklyon 06:31, 18 November 2006 (UTC)
thread_title: A polite request...

message: I have read through the talk here and at microscope to try and get something done about the future of these articles. It seems that people agree on what should be done, it just needs doing!
Here is an outline of what I believe a sensible solution is for the microscopy, microscope and all other related pages:
*Microscope and microscopy should become portal style pages, with summary articles and links to pages on the individual types of microscopy (optical, electron, etc.) and pages on the physical principles of basic microscopes (ie. optics, resolution, electron optics, etc.)
*Microscope should be written from a physical viewpoint, ie. the physics and history of microscopes, as microscopes are the actual instrument. Microscopy should be written from a more practical viewpoint, ie. the usage and reasons for usage of the different techniques.
*Optical microscope needs its own page, similar to electron microscope. Relevant information on individual optical microscopy instruments and techniques need to be moved to this page.
*Each individual microscopy technique and microscope type (eg. phase contrast, scanning electron, etc.) needs its own page, no matter how short - it is better to have a stub for expansion than a long and confusing parent article.
Finally and most importantly:
*microscope and microscopy should be kept short and simple. They are introductory pages to what is a very wide and in depth region of science. Detail should be confined to more focussed articles.
You have a week to make your comments, and, unless there are any major complaints, im going to get started! Zephyris 20:42, 30 July 2006 (UTC)
replies: text: Comment: nothing you propose seems crazy. Why wait? I cannot imagine that anyone will revert you if you make these reasonable changes. Be bold in updating pages. --JWSchmidt 22:05, 30 July 2006 (UTC)
replies: text: Thanks for the confidence boost :D, and i have now let myself loose on the articles, bwahahaha! Zephyris 21:12, 6 August 2006 (UTC)

text: I think that has been done - Marshman 03:51, 30 Mar 2005 (UTC)

text: and it still needs urgent revision. Karol 22:11, 30 December 2005 (UTC)
thread_title: Action

message: Is this link relevant for this webpage (page-owner, self-promotion?)? Pvosta 11:33, 12 September 2006 (UTC)
thread_title:  Link suggestion 

message: Shouldn't these be included? Rotational 05:41, 7 November 2007 (UTC)
thread_title:  Polarised light microscopy and Multiphoton microscopy?? 

message: Is there a page on specular microscopy already, or it is needed to be created? --CopperKettle (talk) 21:47, 27 September 2008 (UTC)
thread_title:  Specular microscopy 

message: This is a new article if anyone wants to have a look. ChildofMidnight (talk) 23:05, 3 August 2009 (UTC)
thread_title: [[Intravital microscopy]]

message: I think this might be of use for illustrating the differences between light and electron microscopy. It's currently used on several butterfly-related articles so if you need to make significant changes that might negatively affect the placement in those articles, I recommend creating a fork:
Papa Lima Whiskey (talk) 11:33, 1 May 2010 (UTC)
thread_title:  Image suggestion 

message: This would ultimately make a great featured article, but still needs a lot of work, as with any article this large in scope of topic. I am removing part of the lead, not because the information does not belong in the article or even in the lead, but to focus initially on the overall readability and usability of the article for the general reader of an encyclopedia. This sentence can be incorporated elsewhere for now, or back to the lead as editors develop it:
:"along with many others including materials science and forensic engineering where microstructure and surface details such as trace evidence are important for understanding properties or the causes of accidents."
While microscopy is fundamental for materials science, it does not necessarily trump its importance in the biological or physical sciences in general. An additional paragraph discussing the importance of microscopy as a technique in the biological and physical sciences plus materials science in particular could appropriately develop the lead section with this sentence. I did leave "revolutionized biology," in because microscopy did that, while it contributed greatly to the revolution of other areas of science. --KMLP (talk) 17:34, 12 October 2010 (UTC)
thread_title:  Lead section 

message: What happened in this sentence: "Staining may also introduce artifacts, apparent structural details that are caused by the processing of the specimen ame extent by specific microscopy techniques that can non-invasively increase the contrast of the image."?
I don't know enough about microscopy to correct this sentence, but it really should be revised. 129.206.24.62 (talk) 13:08, 12 June 2014 (UTC)
thread_title:  Limitations 

message: I'd like to start by thanking those who know something about microscopes for writing into this page for those of us who know little!
I looked this up this page after getting an ad for "affordable atomic force microscopes" because I had no clue about them.  Sadly, after reading the article, I still don't know how on what bases I would choose a microscope if I had the need.  Part of this is needing metrics.  Here is my beginning of a list of the metrics, based on what I (as a non-expert) would anticipate being important:
* finest demonstrated resolution of the microscopy technique in each dimension.  Are width and length equal for all techniques?  I doubt it.  Which techniques can measure depth?
* dimensions and units of measurement - optical microscopy, of course, allows many dimensions aligning with color (subject to diffraction limits etc); is electron microscopy a bunch of bits, or perhaps 8-bit values?
* how much damage the measuring technique does to the sample - atomic-force microscopes seem to scrape the surface; dyes change the chemistry...
* ability of a technique to resolve multiple layers in a sample
* capture pattern, e.g. scanning
* ability to image live specimens
* scanning speed in area/time - square-mm per second I suppose
* restrictions on size or other characteristics of the sample
With that done, I would propose setting up a table listing microscopy techniques and these dimensions, obviously to allow the reader to sort by one (or more?) dimensions of interest.
Schemaczar (talk) 19:23, 8 June 2017 (UTC)
thread_title:  Request some metrics for comparing microscopy techniques, and a table comparing them 

message: I can't find any useful discussion of reflected-light optical microscopy.  While little used in biology for very small targets, it seems very important in other sciences (industry, geology, etc.) and even for botanics and entomolog.--Jorge Stolfi (talk) 19:13, 12 June 2017 (UTC)
thread_title:  Reflected-light microscopy 

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thread_title:  A Commons file used on this page or its Wikidata item has been nominated for deletion