Talk:Histone

message: Please include the contributions of Marie Maynard Daly; ca. 1955 she determined that a major component of DNA-binding histones is lysine, while the predominant idea at the time was that the major component was arginine (see Albrecht Kossel). There is an article for her on Wikipedia that links out to this article, but this article on histones does not reference her. https://en.wikipedia.org/wiki/Marie_Maynard_Daly  — Preceding unsigned comment added by 170.140.104.4 (talk) 22:25, 11 November 2021 (UTC)
thread_title: Historical omission

message: Under the heading 'classes', near the end of the paragraph, it says "During meiosis, through the combination of nucleosome interactions with other proteins, the chromosome is assembled." I can't make sense of this sentence... and don't think it's as clear as it could be. --Seans Potato Business 03:52, 22 April 2007 (UTC)
replies: text: Hopefully it's better now. --chodges 19:03, 6 August 2007 (UTC)
thread_title: Unclear Sentence

message: "There are some variant forms in some of the major classes."  What are classes, and what are variant forms, and how does this relate to the previous paragraph? -Pgan002 02:09, 15 June 2007 (UTC)
thread_title:  Another unclear sentence 

message: I'm confused.
The article's header references chromatin, a DNA based composite within both Prokaryotic and Eukaryotic cells, for the purpose of defining Histones.
To the best of my knowledge, Histone proteins only exist in eukaryotes...
Isn't that misleading? perhaps a proper notation should be made at the opening paragraph, to clarify this.
cheers
Martin fed (talk) 14:22, 4 March 2009 (UTC)
thread_title:  Eukaroya and Prokarya 

message: Could someone add ? LeadSongDog come howl 15:44, 19 June 2009 (UTC)
thread_title:  Need infobox 

message: * core histones – H2A, H2B, H3 and H4
    * linker histones – H1 and H5
H5?
really?
kindly provide reference.
Sameerbau (talk) 20:10, 4 March 2011 (UTC)
thread_title:  classes of histones 

message: Couldnt see any mention of how many of each type a cell might have. 
Is each section of the genome always wound around the same type of histone ? 
What proportion of the cells histones are not wrapped in DNA at the various stages in the mitosis cell cycle ? - Rod57 (talk) 12:18, 15 September 2015 (UTC)
thread_title:  How many of each type 

message: Substituted at 17:58, 29 April 2016 (UTC)
thread_title: Assessment comment

message: I agree that history of histones is needed and I suggest adding the period when histones were found to be just a few types and highly conserved across taxonomically diverse organisms.  James F. Bonner and his collaborators in the 1960s showed isolated chromatin would not support RNA transcription in vitro but if the histones were extracted, RNA could be transcribed.  They went on to develop methods to purify each type of histones, to compare amino acid compositions from peas and calf thymus, and compare sequences to find almost no variation.  Before this period, T'so and Bonner had called together a conference, published in Nucleohistones, a volume of conference papers, in which it was clear that the number of kinds of histone was estimated to be very different by different investigators and that no one know how they would compare when isolated from different organisms. I will add a reference to sequence comparisons to this note in about 10 minutes. <ref>Huang RCC and Bonner J "Physical and Biological Properties of soluble nucleohistones." Journal of Molecular Biology  8(1):54-64 (January 1964)<ref>.LLMHoopes (talk) 23:23, 4 August 2018 (UTC)
thread_title:  More History Needed 

message: =I have added a section of material to the history section describing the analyses of the chemistry and biology of histones in the 1960s by James F Bonner and collaborators and Allfrey and Mirsky.  This addition addresses an imbalance in the treatment of structure of nucleosomes versus the histone molecules themselves and their effects on trancription of RNA in general terms. James Bonner was a member of the National Academy of Sciences and a Caltech faculty member who had published 10 books and over 200 articles in plant physiology (see WP article).  His contributions are an important part of this history. Allfrey and Mirsky suggested regulation by acetylation of histones very early, a phenomenon well established now as shown later in the Histone article.  They showed a general effect but could not show a gene-specific regulation due to lack of the techniques now available such as ChIP.  
LLMHoopes (talk) 09:21, 24 September 2018 (UTC)
thread_title:  Early History of chemistry and biology of histones in the 1960s 

message: The article presently states:
"It is not clear what structural implications histone phosphorylation has, but histone phosphorylation has clear functions as a post-translational modification, and binding domains such as BRCT have been characterised"
There has been at the least one paper that linked some form of phosphorylation on histones with the condensation of chromatin. I read it in a book for graduates not so long ago - if anyone knows the paper, the article could be extended in this regard. 2A02:8388:1604:CA80:3AD5:47FF:FE18:CC7F (talk) 19:21, 14 October 2018 (UTC)
thread_title:  Role of phosphorylation of Histones 

message: Are then all histones and methyl groups removed?
Iff they are, does any slight fold remain even at larger orders of magnitude than a few nucleobases.
During meiosis homologous chromosomes exchange parts.
Is that 100% random or are there biased preferred "junctions" due to chemical differences of the bases or other differentiation.
Watch all HHMI YouTube videos.
If you say something give us official data. Anyone can say lies.  — Preceding unsigned comment added by 2A02:587:4123:AB00:899:26D0:B533:907D (talk) 01:30, 20 September 2019 (UTC)
thread_title:  ''make paragraph'': Meiosis 

message: As of the "07:47, 24 December 2022‎" version of this article -- (the latest version as of the date of this comment) -- there seems to be a TYPO in the caption of the picture in the "Classes_and_variants" section. 
The caption there says:Histone heterooctamer (H3,H4,H2A,H2B) + DNS fragment, Frogbut the acronym "DNS" there looks (to me) like a TYPO.It probably should be [spelled] "DNA" ! ... (right?) 
Please forgive me if I should have just changed it, instead of asking questions here, first. 
By the way, I looked for a possible "mishap" ... by checking to see if that caption had ever contained the spelling "DNA" at some time in the past. Apparently not. I found that: when that caption was introduced -- (along with the picture) -- in a 2-step edit (3 minutes apart) on 24 October 2021 (03:42 and 03:45) ((See: https://en.wikipedia.org/w/index.php?title=Histone&diff=1051576048&oldid=1045209384 )) ... the spelling "DNS" was present right off the bat. 
PS: While I was typing this, some well-intentioned robot (a spell checker of some kind? perhaps) "suggested" correcting the word "heterooctamer" (in that same caption) to "heterotetramer" instead. Now in that case, I rhink the original author of the caption was smarter than the robot. (right?) 
I also did some checking to see whether the character string "DNS" could be found anywhere else in the latest version of this article. The fact that it could not ... "suggests" that the chance of the spelling "DNS" having been intentional (vs. accidental) is remote. 
That "checking" was not 100% conclusive, but ... it was easy to do.(Also; Note: "S" and "A" are close neighbors on a QWERTY keyboard.)
Any comments? Mike Schwartz (talk) 04:57, 22 January 2023 (UTC)
thread_title:  (minor) TYPO? in the "Classes and variants" section   

message: The names nomenclatures used in the table about classification of histone families and variants are not the most up-to-date versions. This table shows names for both replication-dependent and -independent histones in two different nomenclatures (Ej.: H1F0 independent, HIST1H1A dependent). This nomenclature is difficult to understand for non-expert people and it has been recently standardized by the HGNC and MGNC (in mice) in 2022. In addition, the table does not show pseudogenes of histones.
I think the article would benefit from adding this information. If nobody disagrees I can update the table.
As a consequence, I also believe that the names of all histones variants articles should be renamed to the newest nomenclature, redirecting old names titles. Is this something better to discuss in Wikipedia talk:WikiProject Molecular Biology? Are there any similar cases of articles names changed because of nomenclature changes? Aarón Mayo (talk) 15:31, 24 September 2023 (UTC)
thread_title:  Up-to-date and easier-to-understand Histone names nomenclature