Talk:RNA splicing

message: This article needs a considerable amount of expansion, as it is a very important area of cell biology. Also, I move that it be moved to splicing (molecular biology), as genetics usually refers to the mode of inheritance, not the process of gene expression. A disambiguation page should be created for splicing, as it also refers to (to my memory) something to do with the manufacture of ropes. I'll try to make some edits when I have the time and have revised the subject.--ChrisJMoor 02:50, 3 Mar 2005 (UTC)
replies: text: I agree about the need of expansion and, imho, alternative splicing could be merged with this article (unless you're going to really add a lot of new stuff here). However I'm not so sure about renaming this article because genetics, biochemistry and molecular biology are such overlapping terms. --EnSamulili 20:24, 11 Mar 2005 (UTC)
thread_title: Untitled

message: I've expanded the article, introducing sections on splicing pathways, evolution, mechanism, and splicing errors.  I've also added a section on alternative splicing which refers to the main article; this could be used for a merge in the future, but should probably remain as is now that splicing (genetics) is expanded.  I've also added the section splicing (genetics)#Splicing errors for future expansion concerning the kinds of errors that can occur and the diseases they cause.
I'd like to move for a removal of the last section concerning mRNA structure and processing  as I feel that it is off-topic.  This section is described in the mRNA article, where it is probably more appropriate.
--Plociam 00:54, 26 July 2005 (UTC)
thread_title: Expanded Sections

message: Experimental manipulation of splicing using Morpholino antisense oligos has become a common technique in developmental biology.  Splicing can be blocked by targeting a Morpholino to the snRNP binding sites at the donor and receptor sites, or by targeting the nucleophilic adenine.  As I represent the company that manufactures Morpholino antisense, I feel it is better to leave the decision regarding linking the splicing page to the Morpholino article up to a neutral third party.  Here is a reference to the original splice study using Morpholinos in zebrafish: 
The paper is available online: http://www.gene-tools.com/files/draper_etal.pdf
There have been hundreds of subsequent papers using Morpholino-mediated splice modification.  The technique works in embryos and in tissue culture.
jmoulton@REMOVETHISgene-tools.com
23 Jan 06  As there has been no discussion of antisense manipulation of splicing, I have added a section on experimental manipulation of splicing.  I have also made the previous reference callout a live link to PubMed and added additional references.
Jon
A related discusion can be found at Talk:Influenza#Verifyability.2C_understandability.2C_relevance_concerns. WAS 4.250 23:42, 24 March 2006 (UTC)
thread_title: Proposed Added Link

message: I readded that snRNP are pronounced Snurps, as that is how they are commonly referred to in medicine and research. Rrten00 19:50, 14 December 2006 (UTC)
thread_title: Snurps

message: Added explanations for canonical (major spliceosome) and noncanonical (minor spliceosome) splicing. Unfortunately I had to renumber all references manually, since I had to add one at the beginning. Maybe an auto-numbered reference template should be used instead. Tekkaman 12:32, 24 January 2007 (UTC)
replies: text: Fixed it. Samulili 18:15, 24 January 2007 (UTC)
thread_title: Noncanonical splicing; reference problem

message: Since my edit comment was a bit unclear: "However, regardless of which pathway is used, the excised introns are discarded." was removed because some microRNAs and other non-coding RNAs come from mRNA introns. Narayanese (talk) 21:41, 27 May 2008 (UTC)
thread_title:  Discarded introns 

message: I'd like to propose that the following sentence be changed: "Like the major spliceosome, it is only found in the nucleus.[5]" Studies published in 2005 have shown that splicing can occur in dendrites and in human anucleate platelets. Also, the minor spliceosome in particular has been shown to be predominantly located in the cytoplasm in at least one study I am aware of (König et al. 2007, Cell). Darielab (talk) 00:13, 4 July 2009 (UTC)
thread_title:  Minor spliceosome 

message: external links available on this site:
http://highered.mcgraw-hill.com/sites/dl/free/0072437316/120060/ravenanimation.html
under chapter 15.
how splicesosomes process RNA
i don't know how to add it.  —Preceding unsigned comment added by Sameerbau (talk • contribs) 07:43, 11 October 2009 (UTC)
thread_title:  external links 

message: It would be nice to see a couple of figures that show the steps being described in "Self-splicing" and "Spliceosome formation and activity DANK FUCKING MEMES."
Aal42 (talk) 14:37, 4 April 2011 (UTC)
thread_title:  Figures 

message: I don't want to remove the banner myself, but I don't think it should be there. I'm a freshman taking biology in high school, which hardly qualifies as "expert", and I got 90% of what this page what trying to get across.
article is helpful and the resources are reliable.--RawansAljohani (talk) 03:51, 11 September 2018 (UTC)
replies: text: I agree, it already seems pretty simple compared to the actual subjects level. A simpler version can be on Simple English Wikipedia. Benj5378 (talk) 15:45, 13 June 2022 (UTC)
thread_title:  Too technical? 

message: — Assignment last updated by R210240 (talk) 02:54, 16 May 2023 (UTC)
thread_title: Wiki Education assignment: CHEM 378 - Biochemistry Lab - spring 2023