Talk:Bacillus cereus

message: *I've reverted the recent edits made by 192.203.222.78 regarding Gram status as I've been unable to find a source confirming B. cereus as Gram-variable—all of my texts says G+ve (as does my personal experience with Bacilllus strains). With a reputable source (i.e. one whose conclusion isn't drawn from technical errors like old cultures, poor technique, etc), I'm willing to talk.... MarcoTolo 20:21, 12 April 2006 (UTC)
thread_title: Gram status

message: Some suggestions for the development of this and related pages. First, we need to disambiguate the B. cereus sensu stricto species from the B. cereus group. This may require linking to a discussion of the definitions of bacterial species in light of horizontal gene transfer. B. cereus group species differ mainly by their plasmid content, and a few highly specific chromosomal differences, like the conserved point mutation in the plcR gene of B. anthracis. 
Secondly, it should be made clear that the basic plasmid-less bug is an opportunistic pathogen that has an array of enterotoxins and so on, but it doesn't do much damage to healthy people. True pathogenicity is conferred in all cases by toxins on plasmids. Crystal toxins on pBtoxis etc. for B. thuringiensis, the tripartite anthrax toxin on pXO1 for B. anthracis. In B. cereus sensu stricto, it has been shown recently that emetic syndrome is caused by a toxin on a plasmid, pCERE01 (annoyingly, it has been given different names by two different groups), which has a lot of its sequence in common with pXO1 but with a different pathogenicity island. Periodontal isolates also possess distinct pXO1-like plasmids. 
In addition, the conjugative plasmids and a few other mobile genetic elements should be evoked as they can be involved in the spread of virulence determinants and constitute a bit of a sore point for classification in the B. cereus family.
IGollum 01:17, 15 June 2007 (UTC)
thread_title:  B. cereus group, toxins, virulence and plasmids 

message: The article speaks only about "improper cooking", but does not say what might be "proper cooking".
Someone might think 100 °C is enough.  
Bork (talk) 14:03, 30 August 2008 (UTC)
replies: text: "proper cooking"? Currently a sentence reads, "If rice is cooked at, or over, 100 degrees Celsius for 20 minutes or more Bacillus cereus cannot survive[verification needed], therefore eliminating possible food poisoning."  Because of the verification needed, I propose changing the sentence to read as:  Bacillus cereus spores survive the cooking of rice in boiling water, and the spores can germinate and grow either during a slow cooling process or storage at room temperature, thus it is recommended that all cooked rice be stored either in a cold refrigerator or above 60°C cite.  For certain food styles such as sushi where storage of cooked rice at room temperature is required, a Hazard Analysis and Critical Control Point (HACCP) procedure has been devised which coats boiled rice that has been quickly pre-cooled to room temperature within a 30-minute time-window with a 4.47% vinegar, 3.63% sugar, and 1.23% salt mixture to reduce the rice's pH below 4.4. It is claimed this room temperature rice will be safe to consume for 24 hours, after which disposal is required.cite 2 Gzuufy (talk) 01:40, 8 August 2010 (UTC)

text: A partial cite for the sentence as it is currently written.  The cite says, "Temperatures under 212°F can allow some of the spores to survive" (in the sub-section Control and Treatment).  On the prior page for another bacillus the text asserts boiling for 30 minutes kills its spores. Gzuufy (talk) 02:37, 8 August 2010 (UTC)
thread_title: Heat stability

message: Hey all! The doi on the following citation appears to be dead: 
* 
So I went to follow-up and find the actual doi, but I can't find it anywhere. Actually I can't find the article online anywhere. Is anyone able to locate this? Maybe it only exists in print and as such has no doi? I hope someone else can solve the mystery. Thanks guys! Ajpolino (talk) 05:18, 7 February 2016 (UTC)
replies: text: I saw your note here and decided to investigate. To the best of my searching abilities, the article is not available online, although it is indexed in PubMed and other places. Several sources claim to link to the full text online article because JEMS publishes full text online for free. However, the JEMS online archives only go back to 2008, while this article is from 2005. I have no idea why there was a DOI included in the original citation, and as you noted it doesn't work at all, so I have removed it. Fortunately, I was able to track the article down in a library and can attest that it does exist and supports the cited statement. It's actually quite a good, simple source for the practical side of B. cereus food poisoning, and I might use it to improve the article a bit if I have time later. Thanks for pointing this out! A2soup (talk) 03:00, 15 February 2016 (UTC)
replies: text:  Great! Mystery solved!! Ajpolino (talk) 03:02, 15 February 2016 (UTC)
thread_title:  Broken doi source 

message: — Assignment last updated by ICapt.NemoI (talk) 19:23, 19 October 2022 (UTC)
thread_title: Wiki Education assignment: MIBO 3500 Introduction to Microbiology