Talk:Thin-layer chromatography

message: On what basis does tlc separate chemicals? Polarity? Charge? Size?... Aaadddaaammm 05:46, 3 September 2006 (UTC)
replies: text: Mostly polarity. --Slashme (talk) 10:57, 30 June 2008 (UTC)
thread_title: Basis

message: Hi.  I just merged the AS page with this one, as per request.  If someone who has knowledge on this subject could cleanup my edits, I'd appreciate it.--C.lettinga 05:32, 15 December 2006 (UTC)
thread_title: Apropriate Solvant merger

message: I made some changes in the explanation of the TLC theory. It was wrongly explained. I hope everybody agrees with this Krulkrul 13:41, 28 October 2007 (UTC)
replies: text: The part about complete reversal of elution order was indeed problematic at best. However, it looks like you've omitted (or removed by oversimplifying) some important parts of how it works. First, you have removed details about how the experiment is run. Second, you have only considered the analyte vs mobile-phase competition (for stationary-phase binding), removing the commentary about partioning effects of the analyte between the stationary and mobile phases. DMacks 21:46, 29 October 2007 (UTC)
thread_title: Explanation of theory

message: This may just be me, but can someone explain to me the purpose of the last sentence in the section Plate Preparation? It just seems redundant: obvious for people who know what stationary and mobile phases are, and yet useless for people who require elaboration on what they are, and particularly the phases in TLC. Nonagonal Spider (talk) 04:53, 16 October 2008 (UTC)
thread_title:  Plate preparation 

message: My lecturer said we were using a partition technique. What does this mean? We used a silica coated plate to compare lipids—Preceding unsigned comment added by 78.144.150.50 (talk) 18:49, 26 November 2008 (UTC)
replies: text: Refers to Partition coefficient of each lipid between the stationary (silica) and mobile (whatever solvent) phases. It's a property that is different among the various lipids, and causes each one to move at different rates along the plate. DMacks (talk) 20:25, 26 November 2008 (UTC)
thread_title:  What is partition? 

message: TLC ist mostly used to identify medicinal plant and extracts thereof for the purpose of authentication and not so much as a general technique to analyze unknown plants constituents. Often TLC methods are compendial protocols e.g. in pharmacopeias.  — Preceding unsigned comment added by Osterluzei (talk • contribs) 17:43, 6 February 2011 (UTC)
thread_title: Medicinal plants

message: The lead section is rather detailed and much of the information could be moved to a different section of the article. As well, it may be good to have a section on different stationary phases (discussing reverse/normal phase and material) as well as another on mobile phases (eluting strength and solvent selectivity groups) to better organize the overall article. I may work on these edits if I get the chance --Dvnyn (talk) 03:02, 7 January 2023 (UTC)
thread_title:  Lead section and organization 

message: — Assignment last updated by RS UBC800 (talk) 21:13, 2 April 2023 (UTC)
thread_title: Wiki Education assignment: CHEM 300