Talk:Gram stain

message: Gram staining is useful, but I wouldn't consider it the most useful stain.  It helps determine the nature of the cell wall, but that alone is not terribly useful when it comes to identifying an organism.  I've changed it to 'most common' because it's the most basic, most taught stain for microbiology.  --Sugarskane 04:36, 20 July 2006 (UTC)
replies: text: The classification of gram negative and gram positive organisms is the essential first step in deciding the nature of an infection and the appropriate antibiotic coverage for an infection before a culture and sensitivity is available. It is the most common stain performed precisely because it is the most useful. - Nunh-huh 04:47, 20 July 2006 (UTC)
thread_title: 'Most Useful' to 'Most Common'

message: Should this article be named gram staining or gram stain? All of the other articles in :category:staining are of the "stain" type, but I see the usefulness in having this article named gram staining. I am undecided. However, I am strongly opposed to renaming the other articles in the category. -- Kjkolb 02:22, 7 May 2007 (UTC)
thread_title:  Name - gram staining vs. gram stain 

message: I am not sure if this stain was originally "invented" to differentiate between those two bacteria mentioned in the article. I was under the impression that it was developed to isolate bacteria in animal tissue, where the bacteria is one colour and the tissue is another. I will attempt to produce documentation of this! :) Rikku (talk) 18:06, 17 May 2008 (UTC)
thread_title:  Original Purpose 

message: This section was removed per WP:NOTHOWTO:
-- œ™ 04:48, 22 March 2010 (UTC)
thread_title:  Section removed 

message: *1a. If Sample is from BROTH: Apply 1 or 2 loopfuls of broth to a clean slide. Air dry. 
*1b. If Sample is from PLATE: Place a drop of water onto a slide. Add a colony to the water and mix. SPREAD out the liquid as much as possible. Air .
*2. Heat fix the slide. Allow the slide to cool.
*3. Stain with Crystal Violet for 30-120 seconds by flooding the slide with stain. 
*4. Apply Iodine solution for 30-120 seconds by flooding the slide with iodine. 
*5. CAREFULLY, decolorize for 1-3 second(s) with Acetone, or as long as needed (10-60 seconds) with 70% IPA/ 30% Acetone.   Immediately after, rinse with water.
*6. Counterstain with Safranin for 30-60 seconds by flooding the slide with stain. Rinse with water.
*7. Blot the slide with a disposable towel, gentle heat OK for the last of the moisture. Remove slide and view organisms using the oil immersion objective of your microscope.
see the results  — Preceding unsigned comment added by 139.190.107.202 (talk) 14:25, 29 May 2012 (UTC)
thread_title: How-To

message: Standard form used by the US Federal Government's Center for Disease Control is as follows:   
:gram
:Gram should be capitalized and never hyphenated when used as Gram stain; gram negative and gram positive should be lowercase and only hyphenated when used as a unit modifier.
:*Gram staining
:*gram negative
:*gram-positive bacteria
It would be helpful for this article, Gram-positive bacteria, and Gram-negative bacteria to be standardized accordingly. Wikiuser100 (talk) 15:03, 17 March 2014 (UTC)
replies: text: I've opened this for broader discussion and resolution at Wikipedia talk:Manual of Style#RfC: Inconsistent capitalization of eponym in same context.  — SMcCandlish ☏ ¢ >ʌⱷ҅ᴥⱷʌ<  06:47, 27 October 2017 (UTC)
thread_title:  Standardized spelling & punctuation 

message: Hello fellow Wikipedians,
I have just added archive links to  on Gram staining. Please take a moment to review my edit. If necessary, add  after the link to keep me from modifying it. Alternatively, you can add  to keep me off the page altogether. I made the following changes:
*Added archive https://web.archive.org/20110716152659/http://www.med-chem.com/procedures/GRAMSTAIN.pdf to http://www.med-chem.com/procedures/GRAMSTAIN.pdf
When you have finished reviewing my changes, please set the checked parameter below to true to let others know.
Cheers. —cyberbot IITalk to my owner:Online 06:39, 26 August 2015 (UTC)
thread_title:  External links modified 

message: When I learnt this, it was 1971, and for counterstaining we used Eosin.  — Preceding unsigned comment added by 124.191.164.107 (talk) 13:00, 21 April 2019 (UTC)
thread_title:  Counterstaning? 

message: We have two sections talking about the same thing, Gram-positive bacteria#Importance of the outer cell membrane in bacterial classification and Gram-negative bacteria#Taxonomy, that I believe are better put together in this article. They both start with "staining is part of traditional taxonomy, in most cases this stain corresponds to this structure", then goes off to talk about the outliers, much of which overlap. Artoria2e5 🌉 01:18, 18 November 2023 (UTC)
replies: text: I don't see the point in this proposal. Gram stain covers what it is, and does not need any further expansion of gram negative and gram-positive bacterial structures. It might be more suitable to do a bit more fine tuning of the mentioned sections. Each article needs to explain their structure ? There seems to be very nearly always some overlap between pages which is mostly unavoidable, imo. --Iztwoz (talk) 22:35, 23 November 2023 (UTC)
thread_title:  Move Taxonomy sections here