Talk:Polyacrylamide gel electrophoresis

message: So isn't this exactly the same thing as a Western-blot ?
Maybe the articles should be fused ? XApple 21:35, 25 February 2007 (UTC)
thread_title: Difference with [[Western_blot]]

message: *SDS is used for two reasons, first it denatures the protein (with the exclusion of disulfide links) such that the secondary, teriary, and quatranry stuctures are not a factor.  Secondly, since proteins can have a positive, negative, or neutral charge SDS (which is a negatively charged detergent) standardizes the protein.  Hope that helps! -- Robert Stone, Jr. 00:15, 1 January 2007 (UTC)
thread_title: Why SDS is used

message: some info on native PAGE would be nice 202.36.134.22 04:03, 31 October 2007 (UTC)
thread_title: native PAGE

message: Is it true, that only proteins can be separated with this technique? Why not any molecule?  —Preceding unsigned comment added by 130.225.22.254 (talk) 14:15, 22 March 2009 (UTC)
replies: text: Theoretically, all mixtures containing anions  can be sampled. Yet, the gel has relatively huge pores for the huge protein molecules. The gel is not suitable for separating smaller anions. Furthermore, electricity is applied to the cell, meaning simpler anions will probably just be broken down through electrolysis. Lastly, detection is often visual which involves sticking coloured or fluorescent dyes to the proteins. It is highly unlikely substances like coomassie brilliant blue will colour just any random anion! Be my guest to look anything mentioned up. Redtails (talk) 16:32, 18 June 2009 (UTC)
thread_title:  Which samples can be used? 

message: SDS PAGE requires that proteins to be run are denatured to thier primary structures and coated with the SDS detergent so that charges and native folding are not factors, only primary structure length which is directly related to weight.  I would like to contest the statement in the intro paragraph that states that the proteins mobility in a PAGE gel is a function of "high order protein folding".  This is incorrect for the reason stated above.
Prudhommei1 (talk) 19:04, 24 September 2009 (UTC)
thread_title:  Issue with intro paragraph 

message: While the Procedure section as it stand now looks very professional, I do have some nits to pick, since there are some odd (in my experience) things in there. I've done Westerns in half a dozen different labs (and thus used half a dozen different protocols), but there are two things I've never heard before. (1)Degassing the gel solution, and (2) the use of different buffers for anode and kathode. Where do these come from? Just wondering, as both seem totally unnecessary to me. Also, I would add to the first section the heating of the sample to >90 degrees C to aid denaturing, in my experience universally done. 82.21.156.156 (talk) 20:25, 27 September 2009 (UTC)
thread_title:  Protocol oddities 

message: The figure caption on the first gel on the page says "Picture of an SDS-PAGE. The molecular marker is in the left lane". The molecular marker page linked talks only about DNA markers. Since this is an SDS-PAGE gel it should be a protein ladder.  — Preceding unsigned comment added by 137.186.174.29 (talk) 04:26, 10 July 2011 (UTC)
replies: text: Removed link Hempelmann (talk) 06:08, 20 July 2011 (UTC)
thread_title:  First figure 

message: This article is pretty much only about Laemmli-type discontinuous SDS-PAGE, and mostly ignores all other types of SDS-PAGE (different buffer systems, non-discontiuous, Schaegger-type, etc.) Although disc. SDS-PAGE is probably the most used, it is not the only variant. Currently the article gives the impression that SDS-PAGE is synonymous with Laemmli gels. I will try to address that, but further input is very welcome. Thanks, Rainbowwrasse (talk) 12:51, 13 October 2011 (UTC)
thread_title:  ignores most variants 

message: I have PhD in molecular biology and run SDS PAGE gels for a living
I don't edit wiki cause I don't like the idea that work i do for free can be used by for profits, and cause i'm tired of re re re correcting stupidity
whoever is responsible for this page
take out all those stupid graphics showing weighing out chemicals
learn something about SDS page, and update the refs 
etc
good luck (eg, coomassie may still be the most popular stain, but it is now often used in non organic (aq) solutions, not with methanol; the ivgn fluor dyes are catching up;  — Preceding unsigned comment added by 50.195.10.169 (talk) 15:19, 29 November 2012 (UTC)
replies: text: As you are an expect why are you reading the article if you you do not intend to contribute to it? For those readers who have never used the method, or seen it demonstrated, the graphics are useful. What aspects of SDS PAGE do you think are missing and need to be learned about? Graham Colm (talk) 19:15, 29 November 2012 (UTC)
thread_title: lousy 

message: I've edited this article to expand its scope to include all types of polyacrylamide gel electrophoresis and not just SDS-PAGE, since there is so much overlap between all these methods.  See discussion at Talk:Gel electrophoresis#Unmerging Agarose Gel Electrophoresis?. Antony–22 (talk⁄contribs) 01:55, 27 June 2013 (UTC)
thread_title:  Article scope expanded 

message: I think there is a need for a separate page for SDS-PAGE, mainly because that is the most performed of the PAGE.  This page can be a general introduction for the PAGE, theories, and forms of PAGE, use of PAGE for DNA electrophoresis (e.g. in DNA sequencing), etc. Hzh (talk) 11:38, 1 October 2013 (UTC)
thread_title:  SDS-PAGE