lflage/wiki-talks huggingface dataset:
Talk:Insertional mutagenesis

message: First, Isn't there an more general term than 'onocogene' for the inserted viral DNA? 
Second, Isn't the term 'onocogene' a mispelling of 'oncogene', which refers to a cancer-associated gene?
Third, Don't the preferred insertion sites of different viruses vary with the type of virus, as opposed to all viruses inserting their DNA next to the 'c-myc' gene?
Forth, Isn't it true that many viruses do not remain latent within a cell?
Fifth, Isn't the 'bursa of fabricus' present only in birds, while viruses affect more than birds?
Briancady413 (talk) 21:08, 17 November 2009 (UTC)
thread_title: Needs Work
Talk:Ka/Ks ratio

message: This article does not make it clear what is meant by a synonymous or non-synonymous site; the definitions of these also seem to be non-obvious (see http://pubmlst.org/software/analysis/start/manual/dsdn.shtml).  — Preceding unsigned comment added by 24.213.110.213 (talk) 19:35, 16 December 2012 (UTC)
replies: text: I came to the talk page to make exactly the same point (ask the same question). i.e. what is a synonymous vs. a non-synonymous site? By site I take it to mean a column of a multiple sequence alignment, but how is a site synonymous or not? --Dan Bolser (talk) 22:10, 9 November 2014 (UTC)
replies: text: Further to the Ars Technica article mentioned in the header above, I came to this article to see if its readability could be improved for general readers. However, despite being a biologist (trained last century, admittedly), I came unstuck on this same issue that the two previous commentators did. I understand what synonymous means in the context of base changes (and that'd be easy to articulate for a general reader), but I can't work out what a synonymous site is. It's not helped by the fact that there's no linked article, nor are the articles that are linked helpful on this point. Can anyone advise? --PLUMBAGO 09:37, 6 January 2016 (UTC)

text:  Me too. I'm pretty used to improving biology articles, and am similarly qualified to User:Plumbago, but John Timmer had a point in his Ars Technica article about this particular Wiki-specimen. It's a bit embarrassing to have Signpost (6 Jan 2016) pointing to bad science writing here, so perhaps we can work out what this article was trying to say, and do it a little less "impenetrably". At the moment, it's frankly at mermaid level. Chiswick Chap (talk) 12:59, 16 January 2016 (UTC)

text:  In case it helps, the number of synonymous "sites" is calculated as the number of possible changes in a base that would be synonymous. Any base can change to any of the three other bases. A codon (three bases) can thus change to 9 possible codons (any one change in one of the three bases). The number of synonymous sites was originally calculated as the number of possible codon changes that are synonymous (same for non-synonymous sites). This is the simplest model assuming that all of the 9 base changes are equally likely (Nei & Gojobori's version of dN/dS, 1986). However, since not all base changes are equally possible, more advanced maximum-likelihood methods take the different mutability of each base into account in calculated the relative neutral probability of synonymous and non-synonymous changes. 185.118.205.91 (talk) 08:47, 11 May 2018 (UTC)
thread_title: Synonymous sites
Talk:Kozak consensus sequence

message: Yes they are the referring to the same thing so should be merged. The Kozak sequence adds little and could be deleted and point to the Kozak consensus sequence article.
TransControl 03:03, 25 May 2007 (UTC)
thread_title: Merge of [[Kozak sequence]] with this page

message: I explained what the brackets, R and lower case letter stood for.
Blueawr (talk) 10:58, 24 August 2012 (UTC)
thread_title: Untitled
Talk:Mysore Airport

message: The article talks of Kingfisher Airlines that's long gone. SpiceJet has introduced flights here.   — Preceding unsigned comment added by 101.62.212.46 (talk) 18:06, 26 December 2012 (UTC)
thread_title: Update Required

message: I propose that Mysore Mandakalli Airport be merged into Mysore Airport. I think that the content in the Mysore Mandakalli Airport article is alomost same as Mysore Airport. Both articles are about same topic.- Prasannatb (talk) 08:46, 23 November 2011 (UTC)
thread_title:  Merger proposal
Talk:Microbial genetics

message: Hello wikipedia community, I will be editing this page for AdGen UWO. I will be adding a brief overview of the genomes found in the microorganisms; Bacteria, Viruses, Protozoa and Fungi, and Archaea. I will also be expanding further on the applications of Microbial genetics and how they have contributed to Science, Technology and Society.
(Ivorobi33 (talk) 23:16, 12 October 2015 (UTC)
replies: text: Great improvement. T.Shafee(Evo&Evo)talk 02:15, 14 January 2017 (UTC)
thread_title: Untitled

message: Overall the article seems well written but it could use some improvement. The lead paragraph needs more information to provide a general introduction to the topic. Another crucial area of improvement needed is the amount of information in each section needs to be balanced. The virus section is lacking the adequate amount of information. The article needs more information on how genetics impacts microbial organisms. 
The article is neutral and does not present the authors perspective which is good. Rnp2w (talk) 18:32, 13 October 2018 (UTC)
thread_title:  Peer Review
Talk:Microsatellite instability

message: Propose rename of Structure section to Definition - since that it what it seems to be about. - Rod57 (talk) 19:00, 12 July 2016 (UTC)
thread_title:  Propose rename of Structure section to Definition 

message: Need details of how it is tested. eg is it detectable in biopsy micrographs ? Are the micro-satellites in the cell nucleus or the cytoplasm ? Does it need a tumour biopsy ? Does it need DNA sequencing ? etc etc - Rod57 (talk) 19:04, 12 July 2016 (UTC)
thread_title:  Need details of how it is tested 

message: Latest Immunotherapy Research in Treating Colorectal Cancer quotes "But, clearly, the nivolumab data in conjunction with prior data with pembrolizumab in the same population really cements this concept that immune checkpoint therapy with PD-1 in MSI-high colorectal cancer is a fantastically active option." (further testing needed) - Rod57 (talk) 19:10, 12 July 2016 (UTC)
thread_title:  MSI-high status could indicate use of immunotherapy
Talk:Modifications (genetics)

message: Added sources 2 and 3 to the document. Mp6180 (talk) 05:49, 19 September 2019 (UTC)
thread_title: Untitled

message: I am not sure what this article is about. The lead appears to contradict the body as it says it is different to mutations and not inheritable, yet the body uses these concepts to explain the modification. It appears to be a conflation of genetic engineering, artificial selection, evolution and natural variation.  Aircorn (talk) 20:17, 23 September 2021 (UTC)
thread_title:  Confused
Talk:David Campbell (Australian politician)

message: Reworded but maintained this, it was not giving a NPOV previously, as it made claims (not simply spoke of) of Campbell being inept and this view being held by 'many', and did not cite that this view was held by the NSW Opposition lead by Barry O'Farrell.  —Preceding unsigned comment added by 115.64.132.151 (talk) 13:52, 14 April 2010 (UTC)
thread_title: "Mr. Slow"

message: This article needs a date of birth --Philip Baird Shearer (talk) 08:52, 22 April 2008 (UTC)
replies: text: TV News report said he was 52, so born in ≈1958. Need a confirming reference still. --220.101.28.25 (talk) 20:59, 20 May 2010 (UTC)
thread_title: Date of birth

message: He is known as "David Campbell", and his middle name is commonly known or used, so this should be reflected in the name of the article. I would suggest "David Campbell (Australian politician)". Cyril Washbrook (talk) 09:32, 22 May 2010 (UTC)
replies: text: Yes, that would be better. - Shiftchange (talk) 13:57, 22 May 2010 (UTC)
replies: text: There is still some confusion here. The article is called David Campbell. Yet the intro sentence has him listed as David Andrew Campbell. The info box has him listed as Andrew David Campbell. I understand the name of the article. But we shouldn't have confusion with his birth name and the intro sentence. Can someone please advise and/or correct? Jherschel (talk) 11:23, 21 September 2010 (UTC)
replies: text: The article used to be at David Andrew Campbell. It got moved. Problem solved! Frickeg (talk) 11:36, 21 September 2010 (UTC)
replies: text: I know what you're saying, but shouldn't the first line be called Andrew David ("David") Campbell and not David Andrew Campbell? Jherschel (talk) 12:32, 21 September 2010 (UTC)
replies: text: Oh, I see what you're saying. I think you've misunderstood. His name's "David Andrew Campbell", not "Andrew David Campbell" (see here). Frickeg (talk) 12:43, 21 September 2010 (UTC)
replies: text: Changed info box birth name to David Andrew Campbell Jherschel (talk) 14:18, 21 September 2010 (UTC)
thread_title: Article name
Talk:Nick translation

message: Er, how kan the Klenow Pol I fragment translate from a nick onwards? The Klenow fragment doesn't have 5' 3' exonuclease activity, hence it cannot remove the downstream nucleotides.
And a question concerning the second part: when DNase I nicks the dsDNA strand, it does so at random places, thus on both sense and antisense strands. But during polymerisation by Pol I, doesn't the progressive 5' 3' exonuclease eventually lead to a ds break? (Of course assuming that there is a nick downstream from the first, but on the other strand.) 84.197.145.92 16:33, 3 June 2007 (UTC)
thread_title: Er
Talk:Cavalry regiments of the British Army

message: In the (excellent) table showing the amalgamation of cavalry regiments from 1922 to 2015, the 9th/12th Lancers appears in the table as having amalgamated in 1922.  It amalgamated in the 60s.
Would love to help but out of my technical depth!
:Fixed it. Thank you very much for pointing out the error! noclador (talk) 20:27, 5 February 2017 (UTC)
thread_title:  Correction needed to table 

message: I have completed the B class checklist and believe that this article is a Start class article. It could be improved by adding in more in-line citations and more sources or references, as well as the addition of some supporting materials such as an infobox or some pictures. Just a few ideas. If you would like more in-depth comments, please list the article on WP:MHPR for peer review. Once you think it is ready for re-assessment, please add it to the list at WP:MHA. Hope this helps. AustralianRupert (talk) 05:27, 16 April 2009 (UTC)
thread_title: Improvements

message: Especially with the plan of Army 2020.Phd8511 (talk) 09:30, 12 July 2013 (UTC)
thread_title:  Need editing 
Talk:Subtilisin

message: Is this a difference enzyme from Bacillus Subtillis Ehrenberg strain 6051a called Sutilains  — Preceding unsigned comment added by 72.78.186.167 (talk) 17:50, 10 July 2016 (UTC)
thread_title:  Enzyme from Bacillus Subtillis Ehrenberg strain 6051a called Sutilains 

message: Would a kind soul be able to put the site specificity of Subtilisin into this Wiki page?
--Felix Tritschler (talk) 13:36, 31 July 2017 (UTC)
thread_title:  Site specificity ?
Talk:Oncogenomics

message: Any chance we could get that in a "vertical" arrangement?  I think there would be less wasted white space (or blue space) that way.  I have something in mind that does this:
Cancer research box
(one arrow down)
Oncogenomics box
(three arrows down)
Improve box - Prognosis box - Therapeutics box
The last three boxes would need their shapes changed (fewer words per line).  What do you think?  WhatamIdoing (talk) 05:35, 8 March 2008 (UTC)
thread_title: Flowchart

message: Content is outdated and inaccurate.  — Preceding unsigned comment added by 178.118.91.31 (talk) 02:21, 12 January 2012 (UTC)
thread_title: Outdated
Talk:Samuel Bellamy

message: Three WIkipedia articles I read do not agree on the part where Bellamy becomes Captain in around 1716. The Robert Teach and Captain Hornigold articles do not agree with this article on when Hornigold was replaced as Captain and by who.  —Preceding unsigned comment added by 174.57.1.28 (talk) 03:16, 22 January 2011 (UTC)
replies: text: The sources in that are based on the same jumble of unreliable primary sources as above. 134.139.22.143 (talk) 02:00, 21 November 2013 (UTC)
thread_title: Untitled

message: I am a genealogist and a relative of Mary Hallett. She was in fact born in Barnstable but was living in Eastham at the time she met Samuel Bellamy. What we call Wellfleet was at that time an unincorporated village within the town of Eastham. There is a record of the death of a baby boy given the name Samuel whose mother was Mary Hallett in Eastham in 1716. Unfortunately the Barnstable county court house was burned many years ago and court records on her trial are lost. However there is a grave in Eastham for the baby and next to it is the grave of Mary Hallett whose inscription indicates she was born in 1698 and died in 1746. There is no marriage or other children recorded for her at any time. There were no other unmarried or widowed Hallett women of the correct age living in Eastham at the time period with a recorded child who died as a baby. I did find a record of an Elizabeth Bellamy marrying a John Hallett in Barnstable in 1686. Mary's father's name was John. This indicates the strong likelihood that Mary was Sam Bellamy's cousin. Cousin marriage was commonplace on Cape Cod (and still is) at the time. Since this is original research it cannot be included in the article. If someone tells me how to make it possible for this information to be included in the article I would like to pursue that.  — Preceding unsigned comment added by 2603:9000:FF00:8E:75F7:9E61:F857:54F3 (talk) 04:48, 25 June 2020 (UTC)
thread_title:  Mary Hallett
Talk:Daniel Purcell

message: 3 Feb 2013 Brother or cousin...
Listening to BBC Radio Three just now, I heard a passing comment that said "it turns out that the Purcells were cousins". Has new research settled the question? There was also mention to an upcoming episode of Radio 3's Early Music Show (1pm (UK) totday). And a harpsichord piece called "Loves Last Shift", so eveidently thre is more known of Daniel's work than the sketch presently listed at WP. I hope someone who knows can refine the current Daniel Purcell page!
Tkbwik (talk) 09:01, 3 February 2013 (UTC)
thread_title: Untitled
Talk:PBR322

message: Right now the article states: "pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors.".
However had, was it the first vector? What was the very first vector used? I think it would be nice to find out. Perhaps pBR322 was not the first one. 2A02:8388:1641:5500:0:0:0:1 (talk) 18:15, 15 November 2022 (UTC)
thread_title:  Historic first use
Talk:M13 bacteriophage

message: Is it possible to include a diagram of this virus so we can visualize its construction and components, including its method for infecting its host bacteria? It may be important for automatically assembling Quantum dots, so this might be useful information. David Spector (talk) 08:30, 21 December 2011 (UTC)
thread_title:  Diagram? Microphotograph? 

message: M13 is one of the Ff phages (fd and f1 are others), a member of the family filamentous bacteriophage (inovirus). Ff phages are composed of circular single-stranded DNA (ssDNA) which is 6407 nucleotides long encapsidated in approximately 2700 copies of the major coat protein p8, and capped with about 5 copies each of four different minor coat proteins (p3 and p6 at one end and p7 and p9 at the other end) 42.111.166.7 (talk) 13:27, 26 April 2022 (UTC)
thread_title:  M13 

message: — Assignment last updated by FloeEdge (talk) 04:15, 20 October 2022 (UTC)
thread_title: Wiki Education assignment: Virology 2022
Talk:Glider competition classes

message: Is there any reason for the capitalizations?--Vierzehn (talk) 16:02, 25 March 2008 (UTC)
replies: text: If that's the case, then the M should be capitalized in "15 Metre Class" and the like, too.  It would, of course, be "15 m Class" (unit symbols are case-sensitive), but that has no effect on spelled-out unit names.  Why aren't they capitalized? Gene Nygaard (talk) 15:46, 10 March 2010 (UTC)

text: After a bit of research and adding (as a result) of some notes (BTW, please preserve them somewhere, it was a bit of work to collect) I think the history section of Open Class needs rewrite. EB28/29 is I think an extreme design, but it took 1. and 3. in 2010 WGC, so the opinion "extreme does not work" is not so clear anymore. Main train of thought as of now I think needs to be preserved as it is interesting and insightful (extreme vs practical), but the whole thing has to be written from 2012 perspective, probably reporting previous stereotypes. All in all, this is fascinating. Szafranpl (talk) 13:07, 31 August 2012 (UTC)
Talk:Pribnow box

message: Umm, the fiction section... in Neon Genesis, I believe that they're actually referring to the Pribnow Box itself, I don't think that it's "a sort of jargon" at all. I mean the context is that of an alien entity attempting to hack the HQ's control computer, and they discover that the hack is originating from the Prinbow Box. Isn't that pretty closely analagous to the action of RNA polymerase in a viral attack? Not to mention the entire film is an allegory, so I think that it's pretty likely that this was the auter's intention. Anyone have an objection to changing it? [05/04/2007]
I'm going to double my efforts to get around to watching NGE based solely on the above comment. Meanwhile, how in-line are the Pribnow box probabilities with current research/is there somewhere we can easily check? I would assume these probabilities will change as sequences (specifically Pribnow seqs) roll in, regardless of indicative trends in model organisms... Geno-Supremo (talk) 08:36, 20 May 2009 (UTC)
thread_title: Untitled

message: This page does not have sufficient sources. It must require at least 15 to 20 sources to be a good article.
GautamGhosh1996 (talk) 22:35, 5 September 2018 (UTC)
thread_title:  Sources
Talk:Malumfashi

message: MALUMFASHI LIKE MOST NORTHERN STATES HAS A HISTORY OF EMIRATE SYMTEM OF ADMINISTRATION WITH KATSINA EMIRATE AS THE EMIRATE COUNCIL AND GALADIMAN KATSINA AS THE DISTRICT HEAD OF MALUMFASHI.
MALUMFASHI PEOPLE ARE KNOWN FOR FARMING COTTON AND OTHER FOOD CROPS SUCH AS MILLET, CORN, GROUND NUTS, GUINEA CORN ETC. WOMEN IN MALUMFASHI TAKE ADVANTAGE OF THIS TO ENGAGE IN SMALL SCALE PRODUCTION OF GROUNDNUT COOKING OIL WGIC IS SOLD TO AUGUMENT FAMILY INCOME.
TYPICAL OF MOST RURAL AREAS IN AFRICA MALUMFASHI IS GROSSLY UNDERDEVELOPED THEREBY UNDERMINING THE HIGH PRODUCTIVE POTENTIAL OF ITS PEOPLE.
To deal with eventually. Picaroon (t) 00:45, 21 September 2007 (UTC)
thread_title: Block o' text
Talk:Recombineering

message: Are there no simplified pictures we could use to just briefly show the general overview of a recombineering based approach? 84.112.136.52 (talk) 04:20, 19 May 2014 (UTC)
thread_title:  Pictures 

message: Perhaps the article should start with a history of the technique at hand. 2A02:8388:1603:CB00:3AD5:47FF:FE18:CC7F (talk) 16:51, 17 June 2018 (UTC)
thread_title:  History
Talk:Regulatory sequence

message: From the intro: "By doing so, these regulatory proteins can either recruit another protein complex, called the RNA polymerase." They can either recruit a protein complex, or they can... what?
152.133.6.130 (talk) 18:53, 30 June 2008 (UTC)
thread_title:  or...? 

message: Hey All, 
I'm editing this page for AdGen 3595 at the Western University and I plan to add information to this "Regulatory Sequence" Stub with information found from the following sources: 
Hartwell, L.H., et al. (2014). Genetics: From Genes to Genomes. Canadian edition. Canada: McGraw-Hill Ryerson Limited. 
Levo M., and Segal E. (2014). In pursuit of design principles of regulatory sequences. Nature Reviews Genetics. 15, 453–468. 
Lodish, H., et al. (2000). Molecular Cell Biology. 4th edition. New York: W. H. Freeman
Nussbaum, R.L., McInnes, R.R., and Willard, H.F. (2015). Thompson & Thompson: Genetics in Medicine. 8th edition. Philadelphia: Elsevier Inc.
Pennacchio L.A., and Rubin E.M. (2001). Genomic strategies to identify mammalian regulatory sequences. Nature Reviews Genetics. 2, 100-109.
 
Ramaswami, G., et al. (2015). Genetic mapping uncovers cis-regulatory landscape of RNA editing. Nature Communications. 6:8194.
Nmartink (talk) 22:01, 4 October 2015 (UTC)
replies: text: Good plan, it'll be good to have some more information! T.Shafee(Evo﹠Evo)talk 09:08, 5 October 2015 (UTC)
thread_title:  Add to Stub  

message: Do you want to keep the table wrapper for the images in this article or remove it?  If you'd prefer to keep it so that the images scroll together, it'd be best to add "clear: right" (style="clear:right; float: right;") to the style parameter to avoid the issue I mentioned on the AI4 talk page, where the formatting is screwed up when an image or another table is placed directly above the tabled images. Seppi333 (Insert 2¢) 22:28, 10 August 2017 (UTC)
replies: text: Edit this double image table wrapper was also used in Post-transcriptional modification and Gene, so those pages will need to be modified as well. Seppi333 (Insert 2¢) 22:32, 10 August 2017 (UTC)
thread_title:  AI4-templated images
Talk:Variable number tandem repeat

message: "Flanking the repeats are segments of non-repetitive sequence (shown here as thin lines), allowing the VNTR blocks to be extracted with restriction enzymes and analyzed by RFLP, or amplified by the polymerase chain reaction (PCR) technique and their size determined by gel electrophoresis."  Does this make any sense to anyone?  First, this paragraph is one long run-on sentence.  Second, it is unintelligible.  If I had time, I would try to decipher it, but I just don't right now.  I just cannot understand how this came to be submitted like this. Jdevola (talk) 04:27, 2 August 2012 (UTC)
thread_title: Figure Description
Talk:Giannina Braschi

message: This article seems copied from somewhere else, and is very unencyclopedic in tone. Its subject may be notable, but please rewrite in a more neutral tone. Thanks! --lightspeedchick 01:51, 17 February 2007 (UTC)
replies: text: Agree - it needs to meet standards for a biographical article, not a literary essay. Working on it.Parkwells (talk) 13:50, 26 April 2019 (UTC)
thread_title: Untitled

message: Spanglish is a dialect, not a standard language. Lestrade (talk) 17:32, 1 November 2011 (UTC)
thread_title: Spanglish

message: Established standard headers for a biographical article, which this is supposed to be. Put life events in relatively chronological order. Try to organize literary career by chronology; reduce repetition about books. Need to move literary discussion to overview or related. Put lengthy reviews of 'US of Bananas' in the article on that book. Had too much here. If she is so influential, find references other than her books.  It is questionable where all editions of her books need to be listed here. If there is an article about the book, list there.Parkwells (talk) 16:45, 26 April 2019 (UTC)
thread_title: Editing goals