Talk:Sense (molecular biology)

message: Both strands of the DNA helix serve as templates for RNA transcription of genes. Hence, one of the two strands is (arbitrarily) the plus/forward strand and the other one is the minus/reverse strand. Depending on the direction of the promoter, the plus strand may be the sense or antisense strand for a particular gene and the minus strand may be the antisense or sense strand for another gene. Mixing these two nomenclatures is highly misleading.  — Preceding unsigned comment added by 153.96.86.132 (talk) 10:09, 27 October 2015 (UTC)
thread_title:  Sense/Antisense and Plus/Minus 

message: In my experience "plus" and "minus" have been used to refer to the orientation of a sequence relative to genomic assembly, while "sense" and "antisense" are relative to an individual gene's transcription direction. I think this page needs to be corrected. Madeleine ✉ ✍ 18:39, 24 July 2009 (UTC)
replies: text: People kept editing it back and forth and I finally realized why. I think textbook convention is to write the 5'-->3' antisense DNA strand on top and the complementary 3'<--5' sense DNA strand on the bottom, but if you're a researcher, you care about the sense strand and that's what you use in documents and programs. Since translation from mRNA is 5'->3', it makes sense to write the (basically same sequence) sense DNA strand in the 5'->3' direction as well. Ultimately, it does not matter which strand is on top or bottom. Only two things matter: (1) which strand has the sequence for the amino acid codons and (2) the inherent direction of the bases (the 5' end and the 3' end). I made a note about it. Please clarify anything that could confuse people, but do not delete the note without replacing it with something better. I agree with anonymous 62.83.140.65, this is a fundamental concept. We need to explain it right. --Flecha2 (talk) 22:28, 16 December 2010 (UTC)
thread_title:  Plus vs. sense 

message: Sense sounds very similar to directionality (molecular biology). Please explain the differences in the article. -Pgan002 (talk) 13:52, 24 February 2011 (UTC)
replies: text: In a nutshell, "directionality" has meaning for strand of DNA or RNA, whereas "sense" usually describes the relationship between a strand of DNA or RNA and a coding sequence.  This article needs a lot of work, but I'm afraid I don't have time right now.  Adrian J. Hunter(talk•contribs) 14:06, 24 February 2011 (UTC)
thread_title:  Relationship to directionality? 

message: "Sometimes the phrase coding strand is encountered" Does this statement refer to the sense or the antisense strand? It is not clear in the article.72.93.188.84 (talk) 22:10, 28 June 2011 (UTC)
thread_title:  Which is the coding strand? 

message: In the schematic the mRNA is showing in the cell nucleus. For me, there is a previous RNA made in the cell nucleus and then outside it is converted into the mRAN.  — Preceding unsigned comment added by 81.65.6.19 (talk) 10:45, 19 April 2012 (UTC)
thread_title:  The Schematic showing how antisense DNA strands can interfere with protein translation. 

message: Since someone put a merge template on Sense strand I would like to argue about it here.  I like to put in links for terms like Sense strand or antisense strand and linking to Sense (molecular biology) just slows down people who want to know what the term means. Graeme Bartlett (talk) 09:19, 22 May 2012 (UTC)
thread_title: merge of Sense strand

message: Translation starts from 5' to 3' (the start codon is 5'-AUG-3'). In the table the RNA sense is wrong because it has its start codon at the 3' end and it should be at the 5' end. I mean the mRNA can't end in GUA-3' because the 5' end connects with the ribosome to initiate the traslation (not the 3' end). It could be 3'-...XXX-XXX-GUA-5' or also 5'-AUG-XXX-XXX...-3' but never 5'-...XXX-XXX-GUA-3'.--Miguelferig (talk) 18:28, 11 July 2013 (UTC)
replies: text: You are correct.  Putting a start codon at the end of a sequence like that is misleading anyway, as real mRNA molecules begin with a 5'-UTR, not a start codon.  I've removed the sequence and references to it. Adrian J. Hunter(talk•contribs) 11:27, 12 July 2013 (UTC)
thread_title:  Start codon is wrong 

message: I've fixed it. The Sense mRNA should be the one complementary to the antisense (template) DNA and "identical" to the sense/coding DNA.  — Preceding unsigned comment added by 5.12.239.4 (talk) 00:06, 22 April 2017 (UTC)
thread_title:  Sense/Antisense mRNA were reversed in the table