lflage/wiki-talks huggingface dataset:
Talk:Digoxigenin

message: It can  easily be attached to oligonucleotides, at either 5' or 3' ends by chemical modifications. 
To detect mRNA production in a given cell : 
1- Prepare complimentary RNA with DIG attached to them. Nowadays, you order that over the internet.
2- Following your preferred protocol for In situ hybridization, attach this probe to mRNA produced by the cell.
3- Use an anti-DIG antibody to detect DIG (and hence, mRNA). 
This is basically rubbish.  I'm not sure how Dig is attached, but it's usually conjugated to a specific nucleotide (normally Uridine) which is incorporated into the complimentary mRNA when it's synthesised - therefore attaching it to the bits where nucleotides join together into strands is unlikely.
Nobody in their right mind would order RNA over the internet; everyone but everyone produces their mRNA fragments in house.  Presumably whoever wrote this is getting confused with primer design, which is (as far as I'm aware) largely done online; given oligonucleotide primers (10-20 nucleotides) and a cDNA library or a complete set of genomic DNA, you can produce a large number of gene fragments (commonly 100-1000 nucleotides) using PCR; which is usually put in a plasmid.  You then use an RNA polymerase, and a nucleotide mix containing only Dig-labelled Uridine triphosphate, to make a transcript that can be detected by antibody.
It can also be conjugated to specific sugars and allowed to incorporate into glycoproteins in live cells, and detected by anti-Dig antibodies.
Am updating to reflect this, and to make it read less like a protocol for ISH.
Confuseddave 20:03, 12 January 2007 (UTC)
thread_title: Untitled
Talk:Hortonia, Wisconsin

message: I don't understand how this article meets WP:BIO. --Anhydrobiosis (talk) 17:36, 11 July 2010 (UTC)
replies: text: It's not a biography, so why would it meet WP:BIO? If you're talking about notability, consensus has clearly determine that towns, villages, and cities of any size are inherently notable. Royalbroil 03:28, 12 July 2010 (UTC)
Talk:Summative assessment

message: Summative evaluation gives 448,000 google hits. Summative assessment gives 568,000 google hits. Merging "Summative evaluation" to this article is recommended--IsleScapeTalk 20:11, 12 January 2007 (UTC)
thread_title: Merger

message: I've heard the terms "formative evaluation" and "summative evaluation" used in a project context (ie neither education nor psychology). This may mean that separate articles may be required. --Spartakan (talk) 18:20, 4 February 2010 (UTC)
thread_title:  Project evaluation also uses formative and summative 

message: can anyone remove the ad at the end? it's purely commercial...  —Preceding unsigned comment added by 84.59.220.220 (talk) 13:17, 23 July 2010 (UTC)
thread_title:  /* External links */ 

message: Who the hell is Scriven?
This quote needs to be introduced and given some context.
Hwfr (talk) 00:37, 20 September 2017 (UTC)
thread_title:  Scriven (para. 1) 

message: — Assignment last updated by jmenglund (talk) 16:05, 18 October 2022 (UTC)
thread_title: Wiki Education assignment: Teaching and Learning with the Internet
Talk:R.E.X. Records

message: I'm changing a phrase in the article: "[...]though they were especially active in the Christian metal genre some acts (such as Circle of Dust) were exclusively sold in general markets". Exclusively? Circle of Dust, at the very least, was sold in Christian music stores and through Christian music catalogs by the time I became a fan in 1999, which leads me to believe that this sentence is either false or that these artists weren't distributed directly to Christian establishments until they had their repertoires bought by other labels after R.E.X. went under. Either way, can we look into this more? –BrentonRyan 10 April 2009  —Preceding undated comment added 18:05, 10 April 2009 (UTC)
replies: text: Check for yourself -- Billboard states that they are exclusively carried in mainstream markets, at least in 1995. To be clear, it's in the 4th column on page 40, under the Plankeye photo. "R.E.X.'s approach can be seen in the growing success of the industrial-rock ensemble Circle of Dust, which, while Christian in content, is marketed exclusively to the mainstream." Dan, the CowMan (talk) 01:47, 4 November 2009 (UTC)
Talk:The Hundred Year Association of New York

message: A user named Chowbok has decided that this image needs to be deleted. In their fairly stern warning, he/she uses some very impressive phrases like "replaceable fair use" and links to a confusing description about what this supposedly means on their user page.  I'm not quite sure I understand it.  Sorry, I'm not a heavy user of Wikipedia, so don't know all the lingo.
I do however, have full rights to use this image for any purpose I see fit -- including uploading it to Wikipedia to illustrate The Hundred Year Association of New York's 2006 Public Service and Scholarship Award Ceremony.  I am the Executive Director of the Association and this image was taken by Amiaga Photography, which was contracted by us for this purpose.
There was some confusion on my part when I uploaded the image and I may have chosen an incorrect classification from the dozens of options in the drop-down menu.  My apologies. If I knew how to fix this, I would. Hopefully, someone in this wonderful community can help me select the correct one.
Thank you in advance.
Lukevl 20:13, 12 January 2007 (UTC)
thread_title: Image of Mayor Bloomberg speaking at the 2006 Awards Ceremony
Talk:Mycobacterium marinum

message: At least some of the references in this article (apart from being in the wrong format) are attributed wrongly, i.e. the numbers don't tie up. Probably needs an expert to sort this out... Smalljim 10:29, 24 March 2007 (UTC)
replies: text: I have to agree that the references have been done out of format and confusing. I tagged the article with a cleanup tag; I don't have time to do it right now. Matt.leeck (talk) 00:19, 11 March 2009 (UTC)
thread_title: References in the article

message: I would REALLY appreciate some information on possible internal infections caused by use of (washed) kitchen utensils in cleaning infected fish tank supplies. 172.191.235.97 00:41, 9 September 2007 (UTC)
thread_title: Internal infections???

message: " ... as M. marinum is a very common atypical mycobacterium causing skin infection (70). " The (70) at the end of this sentence looks like an inline citation to reference number 70 - suggesting that the sentence has been pasted. I haven't found the source. Seventy references would be many for an article - suggesting that the source may be a book. Aa77zz (talk) 17:34, 4 March 2011 (UTC)
thread_title:  plagarism? 

message: Prior content in this article duplicated one or more previously published sources. The material was copied from: https://www.sciencedirect.com/science/article/pii/S0007122699932459. Copied or closely paraphrased material has been rewritten or removed and must not be restored, unless it is duly released under a compatible license. (For more information, please see "using copyrighted works from others" if you are not the copyright holder of this material, or "donating copyrighted materials" if you are.)
For legal reasons, we cannot accept copyrighted text or images borrowed from other web sites or published material; such additions will be deleted. Contributors may use copyrighted publications as a source of information, and, if allowed under fair use, may copy sentences and phrases, provided they are included in quotation marks and referenced properly. The material may also be rewritten, providing it does not infringe on the copyright of the original or plagiarize from that source. Therefore, such paraphrased portions must provide their source. Please see our guideline on non-free text for how to properly implement limited quotations of copyrighted text. Wikipedia takes copyright violations very seriously, and persistent violators will be blocked from editing. While we appreciate contributions, we must require all contributors to understand and comply with these policies. Thank you.  Justlettersandnumbers (talk) 10:57, 25 August 2020 (UTC)
thread_title:  Copyright problem removed
Talk:Lee Patrick (actress)

message: "Patrick died suddenly from a heart seizure on the day before her 81st birthday, at Laguna Beach, California.[3]"
" (November 22, 1901 – November 26, 1982) "
Wouldn't "the day before her 81st birthday" be Nov 21? Jamoche (talk) 04:47, 2 November 2014 (UTC)
replies: text: Finally. — Maile  (talk) 13:47, 1 March 2015 (UTC)
thread_title:  Dates don't add up
Talk:Kimberly, Wisconsin

message: Lukethein, I did not mean to take a chainsaw to your addition but a scalpel would not do.  Kimberly is a village and was referred to numerous times in your edit as a town.  Sources for your material were non existent in places or incorrectly tagged in others, and the massive amount of the data itself should have been split into the already existing sections of the page in order to keep the material relevant to each section.  That combined with a number of typos I caught on the first pass made me think that a clean start would be better than trying to nit-pick your post apart with tons of small edits.
A lot of your info was good.  Please re-enter it with sources and parsed into the existing sections.
Kermit814 (talk) 05:53, 10 December 2008 (UTC)
thread_title: Additional details on Kimberly
Talk:Sajama Lines

message: The coordinates below are in the vicinity of these lines. Some lines can be followed with Google Earth (I have not yet checked all of them); resolution is decent.
-18.27259461473799,-68.71213575564465
Crj 20:24, 12 January 2007 (UTC)
thread_title: untitled
Talk:Heinrich Böll Foundation

message: Anybody has time to add info on this?
All we now have is "The German state did subsidize the work of the foundation with 63 million Euros in 2018." SvenAERTS (talk) 13:07, 23 September 2023 (UTC)
thread_title:  Financial power of the foundation?
Talk:Pasalubong

message: This strikes me as a mere dictionary definition, and a foreign dictionary to boot. Ought it have its own article? Avalon 20:32, 12 January 2007 (UTC)
thread_title: Dicdef

message: The result was no merge.
I vote Don't merge-This is a cultural concept and therefore must be expanded. I think this should be recategorized.23prootie 00:51, 13 January 2007 (UTC)
replies: text: changed vote to "don't merge" as the article has independent RS and has expanded significantly.--Lenticel (talk) 11:18, 14 May 2008 (UTC)
replies: text: I'm boldly closing this as don't merge.--Lenticel (talk) 11:18, 14 May 2008 (UTC)
thread_title: Merge

message: Hey,I was pleased to find this definition here as I'd never heard of such habit before and as partially being related to the Philis, I could learn something new which no one has told me before - thanx !  —Preceding unsigned comment added by Palmozy (talk • contribs) 00:32, 9 December 2008 (UTC)
thread_title:  Surprising entry
Talk:The Devastator

message: Just looked it up on BC basemap, and it's there; but the article name at present is climbers' slang, it seems; the official name should be the one used, unless the summit is widely-known e.g. Mount Cheam vs Cheam Peak.  This page could be a redirect, with its contents relocated to Devastator Peak; entry also needed on the Devastator disambiguation page (which I'll do but will have to be fixed if what I'm suggesting is done....).  I'm going to suggest, also, at the Mountains WiskiProject that the infobox have a "name status" field - official/gazetted, local use, climbers use/guidebooks etc.Skookum1 21:39, 14 January 2007 (UTC)
replies: text: I just moved the contents to Devastator Peak and made this a redirect.  Any further discussion please have on Talk:Devastator Peak.Skookum1 19:15, 15 January 2007 (UTC)
replies: text: I have changed this from a redirect to a disambiguation page. The name The Devastator is not just used in climbing literature but also in geology, such as The Devastator Assemblage. Devastator Peak is known as The Devastator on the GSC website here. Volcanoguy 04:55, 14 October 2011 (UTC)
thread_title: Official name is Devastator Peak
Talk:Ellen's Acres

message: If anyone wants to add to the Ellen's Acres pages, please do so! NoseNuggets 3:40 PM US EST Jan 12 2007.
As a native Nevadan, raised out in the boonies near Tonopah, who used to play with dusters and tires, finding this show just warms my heart.  Is the show still in production?  (I've got no tv.)  Any place to get some neat Ellen gear?  :>  WuShufei (talk) 23:43, 24 January 2008 (UTC)
thread_title: Untitled

message: Ellen's Acres aired on ABC Kids in Australia. I Snuggleblanket (talk) 22:45, 15 April 2017 (UTC)
thread_title:  Australia
Talk:Ellen Corby

message: I question the date for 'The Homecoming' photo (1974). The TV movie aired Dec 1971. I would assume that the photo is also 1971.  — Preceding unsigned comment added by 107.184.68.39 (talk) 10:49, 7 September 2014 (UTC)
thread_title: Untitled

message: Why is there no mention of her 40-year relationship with Stella Luchetta? (2A00:23C4:6390:AE00:2CF3:4DB8:29C0:8F62 (talk) 21:11, 13 April 2018 (UTC)
replies: text: I've added some information.Mighty Antar (talk) 21:58, 13 April 2018 (UTC)
thread_title:  Stella Luchetta
Talk:Robert de la Rocha

message: was/is I think it appropriate to refer to living persons in the present tense. Roberto De La Rocha is alive until proven otherwise. As I gather from http://www.musicfanclubs.org/rage/zack.htm , he has also taken up his creative craft again. I think that's reason enough to refer to him in the present tense.  —Preceding unsigned comment added by 81.242.221.163 (talk) 23:20, 30 May 2010 (UTC)

message: I would like to request to move this article from Roberto de la Rocha to Robert de la Rocha since he was born Robert Isaac de la Rocha. Test0101010101 (talk) 16:42, 13 September 2014 (UTC)
thread_title:  Move request
Talk:Germanisation

message: Tet another anti-German page on Wikipedia. Do you have pages on the intense Polonisation exersizes of Poland in all the surrounding lands (belonging to others) they invaded over the centuries, not least since 1919 and 1945? Thought not. 2A00:23C4:B607:CF00:F92B:9A1:8477:FF6E (talk) 15:12, 22 October 2020 (UTC)
replies: text: "Do you have pages on the intense Polonisation exersizes". Yes. The main article is Polonization. Dimadick (talk) 09:07, 25 October 2020 (UTC)

text: But suppose that we didn't have the article on Polonization, and that subject just happened to have slipped through the cracks, how would that make a bit of difference to the validity of this article, which is sourced to reliable sources? The answer is, it wouldn't, because the existence or non-existence of other articles is not a reflection on the accuracy of this one.  I do not think that Wikipedia is in any way "anti-German", but it is pretty clearly anti-Nazi, not to the extent that we allow our articles on Nazism to be propagandistic or inaccurate -- they have to fulfill the same standards as any other article -- but to the extent that the vast majority of Wikipedia editors reject Nazism, neo-Nazism, and fascism.  In my view, that's not a bug, it's a feature. Beyond My Ken (talk) 08:43, 18 September 2022 (UTC)
thread_title: Anti-German

message: The lead says "" However, this (taken together as a whole) is unreferenced, unsupported by the article body, and incorrect. The first half of the sentence, before the parenthetical, could plausibly either be a mix-up which was supposed to be the other way around (when discussing the German language, Germanisation occurs when non-German words are adopted and adapted into German, and this is, as the parenthetical states, also called Eindeutschung), or else it could be a (slightly confusing but technically accurate) summary of how languages like Czech were Germanised by importing German words (but in that case the unreferenced and unsupported-in-the-article-body parenthetical must be dropped, because Eindeutschung is the opposite process). -sche (talk) 22:45, 29 November 2022 (UTC)
thread_title:  Linguistic Germanisation
Talk:Pierre Spengler

message: According to the infobox, Spengler was born in 1947, married by age 8 in 1955, and divorced by age 10 in 1957. Some fact-checking required. For now, I'm deleting the spouse info.Robbmonster (talk) 06:43, 27 October 2021 (UTC)
thread_title:  something doesn't add up...
Talk:Principle of consent

message: This page appears to be created, not as neutral article on the "Principle of consent" concept (in the context of a hypothetical vote on a United Ireland), but as a soapbox for the musings and thoughts of the creating editor. (Who is a self proclaimed "Independent Socialist Republican" who has stood in local elections on a related ticket). This article needs serious rework to adjust the POV tone, and/or may simply warrant deletion - not just because of the tone - but because the "Principle of consent" concept may not warrant an article of it's own, and could easily be merged into the relevant United Ireland article(s). Guliolopez 21:03, 12 January 2007 (UTC)
replies: text: I've had a stab at re-writing it.  I think it does deserve its own article. Scolaire 16:20, 13 January 2007 (UTC)
replies: text: Good job. Your rewrite demonstrates that -indeed- the concept can be described without bias, and within a standalone article. Guliolopez 19:55, 13 January 2007 (UTC)
thread_title: POV/Soapbox

message: Please do not change "all elected parties in Britain, Northern Ireland and the Republic of Ireland" to "all elected parties in the United Kingdom and the Republic of Ireland." It is not the same thing.  The point being made is that all parties in the Northern Ireland Assembly, as well as all parties at Wesminster, accept the principle.  Scolaire 19:50, 30 January 2007 (UTC)
replies: text: Agreed. Distinction in this case is required, and is important - not just as a point of clarity - but in the context of the concept. Guliolopez 20:25, 30 January 2007 (UTC)
thread_title: Britain, Northern Ireland and the Republic
Talk:Ted Coombs

message: Though TC may be a notable author, most of this biography is rather self-indulgent and non-encyclopedic, not to mention unsourced. I have placed appropriate tagsDGG 21:06, 12 January 2007 (UTC)
thread_title: Untitled

message: User:Tedcoombs - Could someone (dis)prove this? Temperaltalk and matrix? 23:17, 28 December 2007 (UTC)
thread_title: User claiming to be Ted
Talk:Shadow World (role-playing game)

message: Trying to get this page started.  Provided an initial outline loosely adopted from the Forgotten Realms page - feel free to change anything.
Oaxaca dan 00:13, 15 January 2007 (UTC)

message: I've set up several additional pages to cut down on length here - this will be the main strategy: use this page as a general overview and provide the in-depth information on the associated pages.  As this page grows, we can redirect the material into individual pages.
Ones added so far:
*Kulthea
*Emer
*Lords of Orhan
*Jaiman
*Dark Gods of Charon
*List of locations on Kulthea - main page for cities and important places.
*List of Shadow World races - main page for the races found in the SW campaign
Oaxaca dan
::Most of these pages have already been deleted and redirected to Shadow World, and I don't believe most of the rest will ever happen.  In general, I've been removing information that simply restates what's in copyrighted material and made the whole thing a more encyclopedic discussion of Shadow World as a fictional work. Holzman-Tweed (talk) 00:32, 15 January 2012 (UTC)
thread_title: Related pages
Talk:Jan Šejna

message: The schemes of Sejna are given much too credibility throughout the marked section, marked as . wiki-vr 21:19, 12 January 2007 (UTC)
replies: text: Non-compliancy removed after some edits. wiki-vr 08:53, 13 January 2007 (UTC)
thread_title:  Non-compliant 

message: Source: the the Book "RED COCAINE -- THE DRUGGING OF AMERICA AND THE WEST" from Page 116 onwards
=> https://web.archive.org/web/20220309134821/https://portalconservador.com/livros/Joseph-Douglass-Red-Cocaine-The-Drugging-of-America-and-the-West.pdf
in the book are further sources
Opinion: The Books talks to much about: "the Russians are guilty, the Chines people is guilty, the Czech people are guilty" without naming ways to mitigate if one land floods another with illegal drugs to destroy the moral in a country.
=> you can remove this opinion since talk ist not for opinions, but the source above looks valid.
194.230.160.192 (talk) 03:38, 20 October 2022 (UTC)
thread_title:  Does not cite any source
Talk:Avenger (1981 video game)

message: Title- Vic Avenger (Arcade-style action.) Object/Rules- Use your laser cannon to score as many points as you can by destroying the attacking aliens before they destroy you. Three rounds, aliens increase speed they force skirmishes. Scoring- 10, 20 or 30 points for aliens. Mystery points for flying saucers. Libro0 19:13, 2 November 2007 (UTC)
thread_title:  Instructions 

message: There are two different first-party Space Invaders clones for the C64, Avenger and Avengers. They share the same sound effects but have very different graphics (Avenger is wide-screen and the aliens all move in perfect sync, while Avengers is proportioned like the arcade game thanks to putting all the score and control information on the right side, and the aliens each move individually like the arcade game).  I'm not entirely sure which one was released first - this article claims that C64 Avenger was released in 1982, but it has a 1983 copyright date on its title screen, while Avengers has a 1982 copyright date. - Wrassedragon (talk) 12:05, 14 December 2018 (UTC)
replies: text: As far as my research goes, there was "Avenger" SI clone for C64 released (probably) in 1983 - which is a "port" from VIC and subject of this article. I will look for RS about the other "Avengers", so we may add informations about this game to the article (not easy task, as only few magazines are available online and I was born long after this age to remember all this from my own experience). Pavlor (talk) 14:29, 28 December 2018 (UTC)
thread_title:  Avenger / Avengers