Talk:Coombs test

message: The form of the Coombs test may not be specified but it is usually clear from the clinical context or why the test was requested by a physician that the direct or indirect form is referred to.
I understand what you are getting at here but it is a little ambiguous.  Is it really needed?--Ennaear 10:21, 27 July 2006 (UTC)
replies: text: Please make an improvement. Snowman 11:24, 27 July 2006 (UTC)
thread_title:  clarity 

message: I removed the gel electrophoresis reference.  The gel technology used for AHG testing is actually a small chamber filled with polyacrylamide gel.  The gel traps agglutinated RBC's at the top, allowing non-agglutinated RBC's to flow freely to the bottom upon centrifugation.  I am unaware of gel electrophoresis use in this context.  Thanks!--Ennaear 07:46, 28 July 2006 (UTC)
thread_title:  testing methods 

message: Does anyone have any photographs of agglutination?  Perhaps photographs of the gross appearance of agglutination or the microscopic appearance. Snowman 15:05, 28 July 2006 (UTC)
thread_title: Images

message: I made a few minor grammatical fixes (mostly apostrophes) and added a sentence to the opening paragraph describing the most common clinical uses of the Coombs test. Also removed the sentence "A Coombs test is requested by a physician when it is thought that the result will advance knowledge of a patients illness" - as it seems this is true of any medical test. MastCell 17:43, 10 August 2006 (UTC)
thread_title:  Minor revisions 

message: I took off the "Expand" stub... this article seems pretty expanded. MastCell 22:22, 11 August 2006 (UTC)
thread_title:  "Expand" stub removed 

message: Some of the links are said to be double interwiki.  What is this? Snowman 14:28, 4 December 2006 (UTC)
thread_title:  Double interwiki 

message: I think I'm at least average and I couldn't follow this article because it uses so much unexplained language.  I tried to start to fix things but there's just to much.  For example I figure RBCs means Red blood cells but what's RBC's mean. Its worse than a visit to the doctor's office. --Droll 06:49, 14 December 2006 (UTC)
replies: text: It is a complicated subject. It is set out logically. I have removed the tag as I feel the topic has been covered comprehensively. Snowman 09:02, 14 December 2006 (UTC)
replies: text: The aim of the article was start at a basic level and move on for specialised readers. This is in line with the principals of the wiki. Snowman 12:08, 14 December 2006 (UTC)
replies: text: I have rewrote the introduction, which did seem a bit confusing.  Does that help? Snowman 16:27, 14 December 2006 (UTC)
thread_title:  Incomprehesible by non-professionals??  

message: It says in the article that blood can be taken by a phlebotomist, nurse or physician.  Is my medical school unusual in letting students take blood ;) JoncomelatelyCome over 22:48, 28 December 2006 (UTC)
replies: text: I see what you mean, I have reworded that section. Snowman 23:32, 28 December 2006 (UTC)
thread_title:  Situations not covered (TIC) 

message: Consider the following paragraph:
"The direct Coombs test is used to detect red blood cells sensitized with IgG alloantibody, IgG autoantibody, and complement proteins. It detects antibodies bound to the surface of red blood cells in vivo. The red blood cells (RBCs) are washed (removing the patient's own plasma) and then incubated with antihuman globulin (also known as "Coombs reagent"). If this produces agglutination of the RBCs, the direct Coombs test is positive."
I think it's inappropriate to say that RBCs get sensitized by antibodies, rather it's the other way round, meaning, RBCs can sensitize some component of the immune system leading to production of IgM/IgG that gets bound to the RBC surface. Since, it'd be fairly complex to express this principle, and somewhat even beyond the scope of this article, I suggest the following alternative to the above paragraph"
The direct Coombs test is used to detect antibodies or complement proteins bound to the surface of red blood cells in vivo. The antibodies could be IgG allo- or autoantibodies. The red blood cells (RBCs) are washed (removing the patient's own plasma) and then incubated with antihuman globulin (also known as "Coombs reagent"). If this produces agglutination of the RBCs, the direct Coombs test is positive.
Or more simply, sensitized with could be substituted by primed with.
Do let me know what you all think.
Ketan Panchal, MBBS (talk) 08:48, 2 May 2008 (UTC)
replies: text: In certain diseases or conditions an individuals blood may contain IgG antibodies that can specifically bind with antigens on the red blood cell (RBC) surface membrane, and their circulating red blood cells (RBCs) can become coated with IgG alloantibodies and/or IgG autoantibodies. Complement proteins may subsequently bind to the bound antibodies.  A laboratory test, the direct Coombs test, is used to detect these antibodies or complement proteins that are bound to the surface of red blood cells; a blood sample is taken and the RBCs are washed (removing the patient's own plasma) and then incubated with antihuman globulin (also known as "Coombs reagent"). If this produces agglutination of the RBCs, the direct Coombs test is positive. Snowman (talk) 11:02, 2 May 2008 (UTC)
replies: text: I have made some changes to the introduction, which should be a summary for the whole article. Snowman (talk) 11:29, 2 May 2008 (UTC)
thread_title: A Suggestion

message: i've redirected Antiglobulin test here, since it even stated that it meant the same thing. People working on this article, Coombs test, might wish to check the previous version of Antiglobulin test in case there is any info there that is not included here. The stub was very short so someone working on this article should be able to do it quickly. Boud (talk) 20:17, 15 June 2011 (UTC)
thread_title:  Antiglobulin test redirect to here 

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thread_title:  External links modified