Talk:Peptide synthesis

message: This is not sourced and also vague/irrelevant, potentially misleading and/or factually incorrect in parts. I don't know what the table adds (at least without more detail/clarification) Algertzars (talk) 07:53, 8 January 2018 (UTC)
thread_title:  Taken from Fmoc/Boc SPPS comparison section 

message: moved this here as it is all unsourced or badly sourced:
;Solid-phase synthesis
Since its introduction over 40 years ago, SPPS has been significantly optimized. First, the resins themselves have been optimized. Furthermore, the 'linkers' between the C-terminal amino acid and polystyrene resin have improved attachment and cleavage to the point of mostly quantitative yields. The evolution of side chain protecting groups has limited the frequency of unwanted side reactions. In addition, the evolution of new activating groups on the carboxyl group of the incoming amino acid have improved coupling and decreased epimerization. Finally, the process itself has been optimized. In Merrifield's initial report, the deprotection of the α-amino group resulted in the formation of a peptide-resin salt, which required neutralization with base prior to coupling. The time between neutralization of the amino group and coupling of the next amino acid allowed for aggregation of peptides, primarily through the formation of secondary structures, and adversely affected coupling. The Kent group reported that concomitant neutralization of the α-amino group and coupling of the next amino acid led to improved coupling. Each of these improvements has helped SPPS become the robust technique that it is today.
There are two majorly used forms of SPPS – Fmoc and Boc. Unlike ribosome protein synthesis, solid-phase peptide synthesis proceeds in a C-terminal to N-terminal fashion. The N-termini of amino acid monomers is protected by either of these two groups and added onto a deprotected amino acid chain. Automated synthesizers are available for both techniques, though many research groups continue to perform SPPS manually.
;Solid-phase synthesis
The name solid support implies that reactions are carried out on the surface of the support, but this is not the case. Reactions also occur within these particles, and thus the term "solid support" better describes the insolubility of the polymer. The physical properties of the solid support, and the applications to which it can be utilized, vary with the material from which the support is constructed, the amount of cross-linking, as well as the linker and handle being used. Most scientists in the field believe that supports should have the minimum amount of cross-linking to confer stability. This should result in a well-solvated system where solid-phase peptide synthesis can be carried out.
-- Jytdog (talk) 02:54, 7 January 2018 (UTC)
replies: text: I'm trying to clean up this article, I just wrote most of the below as a more concise summary and am trying to cut out waffle. Can we not try and find sources rather than just removing it? Algertzars (talk) 03:35, 7 January 2018 (UTC)
replies: text: Actually your edits were exacerbating the problems the tags described.  You did not add any sources in your edits.  We love experts in Wikipedia but this article has become truly terrible from people just writing what they know instead of editing Wikipedia - which means finding reliable sources, summarizing them, and citing them.  Would you please have a read of WP:EXPERT and follow the advice there?  I do appreciate your desire to improve this article, but adding yet more unsourced WP:OR is not what it needs...Jytdog (talk) 03:39, 7 January 2018 (UTC)
replies: text:  I'm not just writing everything I know - I'm trying to give a concise overview and can add citations. This is very basic material and is covered in most of the text books already cited. It would be more constructive to add citation needed than just remove it. The article is a mess because there is so much badly organized and irrelevant information. I was trying to remedy that broadly first. Algertzars (talk) 03:45, 7 January 2018 (UTC)
replies: text: OK, I will revert back to where you were, and let's see what you can do! Jytdog (talk) 04:36, 7 January 2018 (UTC)
replies: text: Thanks. I'd appreciate you put <citation needed> where you can if there are any more problems - I'm trying to salvage as much of what is already there as I can (even though there is a large amount of unreferenced material). As I mentioned much of it can be referenced using standard text books, but this will take some time.  — Preceding unsigned comment added by Algertzars (talk • contribs) 05:36, 7 January 2018 (UTC)

text: Removed content from here that was transferred back into the main article Algertzars (talk) 04:29, 8 January 2018 (UTC)
thread_title:  Unsourced or badly sourced