Talk:Knockout mouse

message: The picture on this page labeled "knockout mice" is simply a picture of two mice. The same picture is on the Mouse page with the same label. There's really nothing in the picture that suggests that they're anything other than regular mice. A better picture would be of a knockout mouse that shows a physical phenotype. Realisticradical (talk) 16:08, 14 July 2008 (UTC)
thread_title:  picture 

message: In step 1 of procedure section:
 a gene that normal mice don't have and that transfers resistance to a certain antibiotic
Mice aren't sensitive to antibiotics - has this bit been copied in error from an article on bacterial gene knockout? RupertMillard (Talk) 15:46, 14 July 2007 (UTC)
replies: text: it's not too well-explained, but it's not a mistake.  antibiotics like Neomycin and G418 are used as selectable markers because they also kill eukaryotic cells (including mouse embryonic stem cells which are used for this procedure).  i've also always been confused by the fact that they're called antibiotics but that's what people use and i can tell you first-hand that they kill mammalian cells too.  the gene usually used is neomycin phosphotransferase which allows the cells to metabolize the antibiotic and thereby confers resistance.  Roadnottaken 16:18, 14 July 2007 (UTC)

text: That was quick! Thank you for answering my question. RupertMillard (Talk) 16:31, 14 July 2007 (UTC)
thread_title:  resistance to antiobiotic - mistake? 

message: Just a few quick notes. The article lacking full explanations, and no proper references. Subject is highly commercial so NPOV is going to be hard to get right...
Feel we are missing some sections ... history?  Leevanjackson (talk) 23:45, 19 February 2008 (UTC)
thread_title:  MCOTW 

message: KO mouse lines do NOT get fancy names. The NHGRI link provides the information that I just deleted (on Methusalah -the name of a price, not a strain- and Frantic), but is just plain wrong. Correct naming information can be found on the site of The Jackson Laboratory (http://jaxmice.jax.org/findmice/index.html). Some older spontaneous mutants got some fancy names, especially those with cerebellar mutations (staggerer, stargazer, hotfoot, etc), but these nowadays also go by more mundane names. --Crusio (talk) 08:41, 12 March 2008 (UTC)
thread_title:  Fancy names 

message: I found the following quote at
What are the drawbacks of knockout mice? 
While knockout mice technology represents a valuable research tool, some important limitations exist. About 15 percent of gene knockouts are developmentally lethal, which means that the genetically altered embryos cannot grow into adult mice. The lack of adult mice limits studies to embryonic development and often makes it more difficult to determine a gene's function in relation to human health. In some instances, the gene may serve a different function in adults than in developing embryos. 
Knocking out a gene also may fail to produce an observable change in a mouse or may even produce different characteristics from those observed in humans in which the same gene is inactivated. For example, mutations in the p53 gene are associated with more than half of human cancers and often lead to tumors in a particular set of tissues. However, when the p53 gene is knocked out in mice, the animals develop tumors in a different array of tissues. 
Either this website is quoting the Wikipedia article (section "Limitations") or vice versa.
Mirughaz (talk) 21:00, 7 May 2009 (UTC)
thread_title:  Unattributed quotation 

message: Hello fellow Wikipedians,
I have just added archive links to  on Knockout mouse. Please take a moment to review my edit. If necessary, add  after the link to keep me from modifying it. Alternatively, you can add  to keep me off the page altogether. I made the following changes:
*Added archive https://web.archive.org/20100611201320/http://molpharm.wisc.edu/Faculty/fac_pages/gould/Pub12754522.pdf to http://molpharm.wisc.edu/Faculty/fac_pages/gould/Pub12754522.pdf
When you have finished reviewing my changes, please set the checked parameter below to true to let others know.
Cheers.—cyberbot IITalk to my owner:Online 04:58, 26 February 2016 (UTC)
thread_title:  External links modified 

message: Although this article is longer/more thorough, some of the same information is found in Gene knockout and in several related articles, for example Gene knockdown, Gene knockin, and Conditional gene knockout.  I wonder if we really need so many articles with very similar information. --Hordaland (talk) 18:46, 20 March 2016 (UTC)
thread_title:  Merge? 

message: Substituted at 21:20, 29 April 2016 (UTC)
thread_title: Assessment comment