Talk:DNA polymerase

message: This article was the subject of a Wiki Education Foundation-supported course assignment, between 12 May 2020 and 22 June 2020. Further details are available on the course page. Student editor(s): Cstaheli. Peer reviewers: Mkalarobinson, Km86867. 20:55, 19 January 2022 (UTC)
thread_title: Wiki Education Foundation-supported course assignment

message: In the article it says that it acts both as the Primase enzyme and the Polymerase. According to pg567, World of the Cell (Becker et al, 2006, Benjamin Cummings) although the Primase and the Pol α are closely asssociated they are separate proteins. I've looked through some other literature but its mostly older stuff so is fairly ambiguous.GiollaUidir 08:18, 20 October 2006 (UTC)
replies: text: Infact, I've found another source that says they are separate: Figure 4-36, pg136, Molecular Cell Biology, Lodish et al., 2004, W.H. Freeman and Company. I'll edit when I get a chance. (Guess who's in the middle of an essay!? :P)GiollaUidir 09:03, 20 October 2006 (UTC)
thread_title: DNA Polymerase α

message: "Nuclease is a great advocate for DNA replication." ... maybe it's just today, but I have no idea what that is actually trying to say. Nuclease CLEAVES DNA strands so I don't think you want too much of it around during replication. Jimhsu77479 (talk) 06:50, 6 March 2009 (UTC)
thread_title: Confusing sentence

message: Any idea what actually removes the RNA primer from the DNA strand? Pol I in prokaryotes as far as I can see but I can't find what removes it from eukaryotes.GiollaUidir 08:37, 20 October 2006 (UTC)
replies: text: Yes, I'm talking to myself. 'tis Proliferating Cell Nuclear Antigen-Replication Factor C-DNA Polymerase δ (PCNA-Rfc-DNA Pol δ-a complex formed to stabilise Pol ’s binding to the template) from: Figure 4-36, pg136, Molecular Cell Biology, Lodish et al., 2004, W.H. Freeman and Company.GiollaUidir 09:47, 20 October 2006 (UTC)
thread_title: Primer removal

message: You introduced what the enzyme does, but should have also mentioned the different types in prokaryotes vs eukaryotes. There should be a reference to the possibilities of mutation and what could happen if that takes place. Referring to its importance in lab procedures is not important to the wider audience that may be trying to understand holistically what a DNA polymerase protein is and does.
Arjbajarj (talk) 05:29, 4 February 2014 (UTC)
thread_title: Introduction

message: I've always thought that "leading" and "lagging" refer to newly synthesized strands, not the template strands.  As such, it would seem to me that pol δ and ε are mislabeled.--Estelahe 20:24, 8 October 2007 (UTC)
thread_title: Strand nomenclature?

message: There seem to be lots of different ways to link to pages about individual polymerases used in this article. What is the standard nomenclature / page list? --Dan|(talk) 14:03, 17 December 2010 (UTC)
thread_title:  Nomenclature 

message: Can someone point me at good references describing the active site / catalytic mechanism of the polymerase? Is it a single domain, or do several domains need to work in concert in order to catalyse the addition of a base? Structural differences between the (presumably conserved) catalytic domains of the different enzyme types must be fascinating, so I'd like to start a section on that topic. --Dan|(talk) 14:03, 17 December 2010 (UTC)
thread_title:  Catalytic mechanism? 

message: Please post any suggestions surrounding the merger of Prokaryotic DNA polymerase‎ and Eukaryotic DNA polymerase to the main page of DNA polymerase Abenson9 (talk) 03:21, 13 November 2012 (UTC)
replies: text: Hi, I think, what you meant was, that you want discussion to take place right here. Klortho (talk) 03:24, 13 November 2012 (UTC)
thread_title: Proposed merger of subsections into this article

message: I moved the information below and categorized it by Prokaryotic vs. Eukaryotic polymerases like mentioned above.  I am going to delete the sections below but wanted to preserve the information below somewhere so that if someone disagrees we have a point of reference. Abenson9 (talk) 18:33, 19 November 2012 (UTC)
replies: text: Hi, Amy.  It is a good sentiment, to want to preserve this text, but completely unnecessary!  The way the wiki software works is that all the prior page revisions are all preserved.  It's actually much better and more robust to rely on the wiki software, than copying text here into the talk page, because no one could ever really know, without a lot of work, which version of the page this text that you pasted belonged to.  Just click "View history" on the page, to see what I mean. Klortho (talk) 22:48, 19 November 2012 (UTC)
thread_title: Consolidation/Movement of Information

message: If you haven't seen this yet, please check out User:Diberri's Wikipedia template filling tool (instructions).  Given a PubMed ID, one can quickly produce a full citation that can be copied and pasted into a Wikipedia article. This tool can save you a lot of work and ensure that the citations are displayed in a consistent manner. Cheers. Boghog (talk) 11:04, 2 December 2012 (UTC)
replies: text: Thanks for fixing the reference.  You were correct in assuming that it was the confusion between the two ids.  The paper that is referenced now is correct.  Thanks again! Abenson9 (talk) 02:12, 3 December 2012 (UTC)
thread_title:  Citation suggestion 

message: Please Wikilink POLH, POLI, POLK, POLG, POLQ and Pol ε (epsilon) to POLE (enzyme) .
crated fedility insterter look like typos
Please wikilink Xeroderma Pigmentosum Variant (XPS) to Xeroderma pigmentosum#Types,
perhaps reference with .
The two sentences referencing XPS could probably be consolidated.
The section Polymerase Rev1 and zeta section is lacking refs
The PubMed search review[pt Polymerase zeta]  gives 42 review articles
From a quick look at the abstracts maybe (guessing)
Cell Res. 2008 Jan;18(1):174-83.
DNA polymerase zeta (pol zeta) in higher eukaryotes. PMID: 18157155 
Cell Cycle. 2010 Feb 15;9(4):729-35. Epub 2010 Feb 23.
Multiple two-polymerase mechanisms in mammalian translesion DNA synthesis.
PMID: 20139724
http://diberri.crabdance.com/cgi-bin/templatefiller/index.cgi?ddb=&type=pubmed_id&id=&add_text_url=1
will format refs based on PMID
http://diberri.crabdance.com/cgi-bin/templatefiller/index.cgi?ddb=&type=pubmedcentral_id&id=&add_text_url=1
and on PMC
RDBrown (talk) 12:43, 11 December 2012 (UTC)
replies: text: Thanks for the suggestions.  I thought I had linked back to XPS, but I will definitely do that.  I am currently working on updating the families section and I haven't made it to Rev1 and zeta yet.  What is there currently is what was there originally.  Thanks for the article suggestions as that should make my life a little easier!  I will also link back to the genes of interest as well.  Thanks again for the feedback and hope you think everything is starting to take shape. Abenson9 (talk) 00:40, 12 December 2012 (UTC)
thread_title: Suggestions

message: In the table summarizing DNA polymerase families, it says family C is only found in eukaryotic species.  Yet it (correctly) lists Pol III as an example, which is a prokaryotic enzyme.  Shouldn't the Species column be changed from 'eukaryotic' to include prokaryotic, or even to just prokaryotic?  — Preceding unsigned comment added by 50.55.140.186 (talk) 03:57, 7 January 2013 (UTC)
thread_title:  Polymerase Family C 

message: Please update the article with info on this paper's findings. It may also be relevant to other articles (I'm only adding some info to the article POLQ).
It's featured in 2021 in science like so:
Biologists report that DNA polymerases, long thought to only transcribe DNA into DNA or RNA, can also write RNA segments into DNA. Polθ was found to promote RNA-templated DNA repair, with large implications for many fields of biology.
--Prototyperspective (talk) 10:51, 20 July 2021 (UTC)
thread_title:  Please update with: "Polθ reverse transcribes RNA and promotes RNA-templated DNA repair" 

message: — Assignment last updated by Hcs22e (talk) 22:41, 5 November 2023 (UTC)
thread_title: Wiki Education assignment: Adv Molecular Bio Bass-FSU-Fa23