Talk:Gel electrophoresis

message: Umm, this is mostly about molecules, not about electrophoresis... -- Marj Tiefert (26 March 2002)
:as it should be - gel electrophoresis is used almost exclusively to seperte and characterize molecules; the theory is not of much intrinsic interest.  — Preceding unsigned comment added by 24.60.137.141 (talk) 2:59, 31 September 2006 (UTC)
thread_title: Comment

message: This section should be deleted as the electrophoresis article makes all this moot
USP<726> cites Gel, Disk, Zone among others.  Also, USP<727> Capillary Electrophoresis, as well as Capillary Zone electrophoresis.  Perhaps electrophoresis should not redirect here.
Eh, I'm an idiot, although I do still think Gel electrophoresis is still too specific for electrophoresis to redirect here.Ix 01:47, 31 May 2004 (UTC)
thread_title:  Gel electrophoresis =/ electrophoresis  

message: This section candidate for deletion now that electrophoresis article available
Think electrophoresis should be seperate article.
Electrophoresis refers to the movement of charge particles in a medium
induced by an electric potential, and gel electrophoresis is merely the most
commom application, strongly recommend that electrophoresis not redirect to
this article. Does not adequately explain concepts such as the role of the zeta potential,
sediment potentials, streaming potential, electrophoretic mobility etc, 
nor does it explain related phenomena such as electroosmosis and other electrokinetic actions.
Also does not allow for proper understanding of equations modelling electrophoretic movement
for example Smoluchowski equation and O'Brien and White's model. 
Strongly believe electrophoresis should be a more general article linking to this page
and other applications such as electrophoretic deposition.
:Someone made electrophoresis a separate article recently, FYI. Nathan J. Yoder 22:52, 15 October 2005 (UTC)
thread_title:  Electrophoresis redirect? 

message: I think the first pragraph should give a hint of the modus operandi of the method, ie say that the separation occurs because of different velocity. I also think it would be more explanatory to say that one of the factors that determine the speed is the charge of the molecule, rather than say that it is the isoelectric point. The isoelectric point is just one of the factors that determine the charge that determines the velocity. The other factor is the pH of the medium in which it moves. The pH is normally determined by the buffer that is added. matrix is a word that is often used for the gel or medium in which the molecules move. But matrix is a word that has many meanings in different contexts. gel should be appropriate, or possibly medium. --Etxrge 13:15, 6 January 2006 (UTC)
thread_title:  A couple of suggestions 

message: I think we should mention which particle factors influence migration, like electrical charge, shape, and size. 128.230.44.239 (talk) 02:39, 7 March 2008 (UTC)
thread_title: Factors

message: The article scope is too limited.  It is not _only_ limited to applications of DNA, RNA and protein molecules, eitehr in theory or practice.  See e.g. LUDMILLA SHIRSHOVA and RAGNAR ÖSTERBERG;  “Electrophoresis of podzol soil humic acids”;  Environment International;  July–August 1998;  24 (5–6): pp. 625–628..  —DIV (128.250.80.15 (talk) 07:08, 23 October 2008 (UTC)
replies: text: WP:SOFIXIT -- MarcoTolo (talk) 18:35, 23 October 2008 (UTC)
replies: text: agree - there have been several papers recentlhy on sepration of nanoparticles by agarose gels Cinnamon colbert (talk) 04:02, 18 January 2011 (UTC)
thread_title:  Scope 

message: Another article exists on Agarose gel electrophoresis. The content overlaps considerably with this entry. I'm putting out there the suggestion to merge the two articles. 68.194.106.90 (talk) 20:07, 10 August 2010 (UTC)
replies: text: no; the agarose gel electrophoresis article is crappy, full of errors and typos; this article ain't so great, but its at least a startCinnamon colbert (talk) 02:30, 5 April 2011 (UTC)

text: Yeah all those related pages should be merged into this article with individual sections for them. But the Agarose gel electrophoresis article should be merged into this one, not the other way around, as there are other types of gels used in gel electrophoresis, which don't only use gels made from agarose. The electrophoresis article can be the general info and overview, and can link to this one, which can provide more detailed information on gel electrophoresis. - M0rphzone (talk) 06:40, 26 October 2011 (UTC)
thread_title:  Merge with article on Agarose Gel Electrophoresis?

message: :Continued from WT:MCB#Articles about electrophoresis
I have a couple of comments on the recent changes.  The first is that the discussion of TAE and TBE buffers is relevant to both agarose and polyacrylamide, and the article should reflect that.  Also, I agree that much of the material in the Visualization section can be moved to the applications subarticles and a shorter summary written here.  Be careful not to make the mistake of assuming that nucleic acids are only run in agarose gels; shorter oligonucleotides are better resolved in polyacrylamide gels. Antony–22 (talk⁄contribs) 01:20, 12 June 2013 (UTC)
thread_title:  Further remarks on article improvement 

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thread_title:  External links modified 

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