Talk:Gene expression

message: Southgatea (talk) 21:28, 25 February 2020 (UTC)

message: Need slow throughput techniques e.g. northern blot --Zven 12:12, 17 September 2006 (UTC)
thread_title: Measurement

message: Consider the totality of all gene expressions within a chromosome.
Are all genes on the same side of the DNA strand? In other words if
you split the double helix are all the genes on one strand or are 
they staggered between the two strands?
:If I remember correctly, which I think I do, each strand is just the mirror image of the other one.  Codons on one strand match up with Anti-codons on the other.--Urthogie 21:37, 22 April 2007 (UTC)
replies: text: Genes are dispersed among both strands in a single chromosome.  With 100% confidence. -- Josh 03:39, 15 May 2007 (UTC)
thread_title:  Is human DNA homo- or hetero- encoding? 

message: Why does housekeeping genes redirect to this article? ώЇЌĩ Ѕαи Яоzε †αLҝ 21:50, 11 June 2007 (UTC)
thread_title: Housekeeping genes

message: Was:
* Gene expression, or simply expression, is the process by which a gene's DNA sequence is converted into functional proteins.
But:
* DNA is not 'converted', but transcribed, leaving intact DNA behind
* the term 'DNA sequence' does not capture epigenomic 'information', which may indeed be part of the inheritable trait, or genetic information, and have an effect on the amount or structure of the 'expressed' gene or trait.
* not all 'gene expression', and perhaps not even most, results in protein production. Protein is not the only trait, or 'expression' of genetic information
* Sentence from gene article was more comprehensive, but not quite what we need here. "The process of producing a biologically functional molecule of either RNA or protein is called gene expression, and the resulting molecule itself is called a gene product."
* however, this still needs to be simple, so please continue to refine.
So is now:
* Gene expression, or simply expression, is the process by which the inheritable information which comprises a gene, such as the DNA sequence, is made manifest as a physical and biologically functional gene product, such as protein or RNA.
JetheroTalk 00:49, 9 July 2007 (UTC)
thread_title:  Intro Sentence 

message: I removed the initial lines "The expression of many genes is regulated after transcription (i.e., by microRNAs or ubiquitin ligases), so an increase in mRNA concentration need not always increase expression. In fact, mRNA concentration has been shown to be a poor predictor of resultant protein abundance.[1][2]" because IMHO it serves as a pretty bad start for what essentially should just be a walk-through of the different methods that exists to quantify mRNA (ie. the de facto definition of gene expression in the litterature). Even if we do want to talk proteins, and I agree that this is relevant, it should come in another section. --LasseFolkersen (talk) 12:50, 31 March 2009 (UTC)
thread_title:  Talking about proteins in the measurement of gene expression section 

message: This article is the MCB Wikiprojects Collaboration of the Month.  What does it need?  It appears to me that each of the sections should have a "main article: bla" link in it, for starters.  Then we need to decide how much detail goes into those sections, as each step (i.e. Transcription, Translation, etc.) has its own article already.  Should we be trying to improve the overall understanding of the whole process of expression here?  It's not entirely clear to me what the goal of the article is.  Agathman (talk) 00:11, 28 May 2009 (UTC)
replies: text: The two core questions in any article are "what is it" and "why is it important". The article could do with a better lead that would give a broad and simple overview, less emphasis on techniques (which are of limited interest to most of our audience) and a much expanded section on the various levels of regulation. Tim Vickers (talk) 02:57, 28 May 2009 (UTC)
thread_title:  What's on the to-do list? 

message: This article currently has a significant eukaryoitic bias; please try to avoid this! I will make some edits to clarify the differences between prokaryotic and eukaryotic gene expression, particularly with respect to co-translation in prok's, RNA processing, nuclear export, folding location and secretion via exocytic pathways. - Zephyris Talk 10:33, 29 July 2009 (UTC)
thread_title: Eukaryote bias

message: The previous version of this text explained correctly, that the produced RNA strand is complimentary to the used template DNA strand. DNA "TAC" to RNA "AUG" (encoding for methionine and also serving as start codon for translation) was given as an example. However, the template DNA strand is itself read in "reverse" 3' → 5' direction to create the new strand in 5' → 3' "sense" direction. The newly generated 5' → 3' RNA is therefore identical to the 5' → 3' coding strand of the DNA (complimentary strand of a complimentary strand), with the difference that T is replaced by U in RNA, of course. This new version is in concordance with the more detailed Transcription (genetics) article and, I believe, less confusing to the first time reader. --Firefly's luciferase (talk) 03:01, 26 February 2010 (UTC)
thread_title:  Transcription 

message: The words inducible gene redirect here and yet there's absolutely no trace of the word "inducible" in this article. Someone fix this please or remove the redirect and create a separate article for what an inducible gene is. 98.176.12.43 (talk) 19:34, 9 October 2010 (UTC)
replies: text: The term inducible gene was defined in a previous version of this article but lost in subsequent edits.  I have reintroduced these definitions.  In addition, I have changed the redirect of inducible gene to the more specific Gene_expression#Regulation_of_gene_expression.  Boghog (talk) 20:52, 9 October 2010 (UTC)
replies: text:  I also just noticed that a definition of an inducible gene was included in the Glossary_of_gene_expression_terms#I. Boghog (talk) 07:25, 10 October 2010 (UTC)
thread_title:  inducible genes 

message: Regulation of gene expression#Methods has a more extensive section on tools, albeit it has a focus on whether the abundance measured is relative or absolute (e.i. protein foo @ condition B is n times higher than @ condition A or foo is n pM ), as the article is about change in expression levels. Maybe a copy-paste possibility? --Squidonius (talk) 03:21, 10 October 2010 (UTC)
thread_title:  Tools 

message: Overexpression redirects to this article. What does it mean? --Abdull (talk) 12:29, 2 June 2012 (UTC)
replies: text: Overexpression = gene expression above the normal amount.  This can be done in the laboratory for example by gene knockin (inserting extra copies of the gene into a cell). Overexpression is also a possible mechanism by which pathogens or cancer cells can become resistant to drugs.  Boghog (talk) 12:46, 2 June 2012 (UTC)
thread_title:  ''Overexpression''? 

message: Both the articles refer to the same thing. I see no dissimilarities. The ultimate goal of gene expression is protein synthesis. These two articles are like seeing a snake from its mouth and from the tail. So I propose to merge the two articles. This message will also be posted on Gene expression article. --G.Kiruthikan (talk) 13:58, 1 March 2014 (UTC)
thread_title:  The articles [[Gene expression]] and [[Protein biosynthesis]] are similar 

message: Overexpress and Overexpression redirect to this article but are not defined.  Should the article cover the term or should redirects be removed? pgr94 (talk) 10:41, 12 May 2014 (UTC)
thread_title:  [[Overexpress]] and [[Overexpression]] undefined 

message: Substituted at 15:53, 29 April 2016 (UTC)
thread_title: Assessment comment

message: Long sections on "Transcriptional regulation in cancer" are being added to different articles on genetics. Whether these are WP:UNDUE is a matter of opinion, but that they are similar is not in doubt. It would make more sense to create a single article on that topic, and to link to it with a brief summary in each of the other articles. Chiswick Chap (talk) 07:22, 30 October 2016 (UTC)
replies: text:  Since the section was duplicated in at least 3 articles including this one, I've boldly factored it out leaving a main link and a very brief summary. I'm sure that can be improved upon, and it needs a general reference to a suitable review paper (unfortunately there wasn't one in the text). Chiswick Chap (talk) 08:00, 30 October 2016 (UTC)
thread_title: Multiple overlap

message: Please refer to the following line from the section 3: Measurement
".....ability to quantify the level at which a....."
Can we write "degree" instead of "level"? It sounds more expressive to me. I am not an expert, just a suggestion
Prabhakar Sarma Neog (talk) 13:42, 1 August 2017 (UTC)
thread_title:  Question/suggestion 

message: Not sure if this is the way to ask questions. Apologies otherwise.
    In the section on mechanism there is the following statement:
"The ribosome helps transfer RNA to bind to messenger RNA and takes the amino acid from each transfer RNA and makes a structure-less protein out of it.[9][10] Each mRNA molecule is translated into many protein molecules, on average ~2800 in mammals."
 My question: Are these 2800 DIFFERENT proteins of 2800 COPIES of a protein molecule? can someone clarify.
    Thanks  — Preceding unsigned comment added by 24.251.100.217 (talk) 00:47, 6 January 2019 (UTC)
thread_title:  Question/suggestion 

message: An editor has identified a potential problem with the redirect :Over expression and has thus listed it for discussion. This discussion will occur at Wikipedia:Redirects for discussion/Log/2022 February 5#Over expression until a consensus is reached, and readers of this page are welcome to contribute to the discussion.  Steel1943 (talk) 20:58, 5 February 2022 (UTC)
replies: text: Overexpression and Over-expression are also listed.  Jay (talk) 07:27, 13 February 2022 (UTC)
thread_title:  "Over expression" listed at [[Wikipedia:Redirects for discussion|Redirects for discussion]] 

message: I propose that we remove this article and replace it with a stub containing links to gene, transcription, regulation of gene expression, translation, etc. 
This is another example of redundant information that conflicts with the main articles on the subject. There's no point in having so many articles that cover the same topics - it encourages people to make edits that don't agree with information found elsewhere. Genome42 (talk) 18:32, 2 February 2024 (UTC)
thread_title:  Delete this article