the united states generates a colossal seven million tons of sewage sludge annually enough to fill 2 500 olympic-sized swimming pools while a portion of this waste is repurposed for manure and other land applications a substantial amount is still disposed of in landfills in a new study texas a&m university researchers have uncovered an efficient way to use leftover sludge to make biodegradable plastics in the september issue of the journal american chemical society (acs) omega the researchers have shown that the bacterium zobellella denitrificans zd1 found in mangroves can consume sludge and wastewater to produce polyhydroxybutyrate a type of biopolymer that can be used in lieu of petroleum-based plastics in addition to reducing the burden on landfills and the environment the researchers said zobellella denitrificans zd1 offers a way to cut down upstream costs for bioplastics manufacturing a step toward making them more competitively priced against regular plastics the price of raw materials to cultivate biopolymer-producing bacteria accounts for 25-45% of the total production cost of manufacturing bioplastics certainly this cost can be greatly reduced if we can tap into an alternate resource that is cheaper and readily obtainable said dr kung-hui (bella) chu professor in the zachry department of civil and environmental engineering we have demonstrated a potential way to use municipal wastewater-activated sludge and agri- and aqua-culture industrial wastewater to make biodegradable plastics furthermore the bacterial strain does not require elaborate sterilization processes to prevent contamination from other microbes further cutting down operating and production costs of bioplastics polyhydroxybutyrate an emerging class of bioplastics is produced by several bacterial species when they experience an imbalance of nutrients in their environment this polymer acts as the bacterias supplemental energy reserves similar to fat deposits in animals in particular an abundance of carbon sources and a depletion of either nitrogen phosphorous or oxygen cause bacteria to erratically consume their carbon sources and produce polyhydroxybutyrate as a stress response one such medium that can force bacteria to make polyhydroxybutyrate is crude glycerol a byproduct of biodiesel manufacturing crude glycerol is rich in carbon and has no nitrogen making it a suitable raw material for making bioplastics however crude glycerol contains impurities such as fatty acids salts and methanol which can prohibit bacterial growth like crude glycerol sludge from wastewater also has many of the same fatty acids and salts chu said that the effects of these fatty acids on bacterial growth and consequently polyhydroxybutyrate production had not yet been examined there is a multitude of bacterial species that make polyhydroxybutyrate but only a few that can survive in high-salt environments and even fewer among those strains can produce polyhydroxybutyrate from pure glycerol said chu we looked at the possibility of whether these salt-tolerating strains can also grow on crude glycerol and wastewater for their study chu and her team chose the zobellella denitrificans zd1 whose natural habitat is the salt waters of the mangroves they then tested the growth and the ability of this bacteria to produce polyhydroxybutyrate in pure glycerol the researchers also repeated the same experiments with other bacterial strains that are known producers of polyhydroxybutyrate they found that zobellella denitrificans dz1 was able to thrive in pure glycerol and produced the maximum amount of polyhydroxybutyrate in proportion to its dry weight that is its weight without water next they tested the growth and ability of zobellella denitrificans zd1 to produce polyhydroxybutyrate in glycerol containing salt and fatty acids and found that even in these conditions it produced polyhydroxybutyrate efficiently even under balanced nutrient conditions when they repeated the experiments in samples of high-strength synthetic wastewater and wastewater-activated sludge they found the bacteria was still able to make polyhydroxybutyrate although at quantities lower than if they were in crude glycerol chu noted that by leveraging zobellella denitrificans zd1 tolerance for salty environments expensive sterilization processes that are normally needed when working with other strains of bacteria could be avoided zobellella denitrificans zd1 natural preference for salinity is fantastic because we can if needed tweak the chemical composition of the waste by just adding common salts this environment would be toxic for other strains of bacteria she said so we are offering a low cost a sustainable method to make bioplastics and another way to repurpose biowastes that are costly to dispose of other contributors to this research include fahad asiri chih-hung chen myung hwangbo and yiru shao from the civil and environmental engineering department at texas a&m this research is supported by the kuwait institute for scientific research the ministry of higher education of kuwait fellowship and the fellowship from the ministry of science and technology of taiwan