The International Spy Museum is an independent non-profit history museum which documents the tradecraft, history, and contemporary role of espionage. It holds the largest collection of international espionage artifacts on public display. The museum opened in 2002 in the Penn Quarter neighborhood of Washington, D.C., and relocated to L'Enfant Plaza in 2019.
 Milton Maltz, a code-breaker during the Korean War and founder of the Malrite Communications Group in 1956 (later The Malrite Company), conceptualized the International Spy Museum in 1996 as a for-profit organization. The original museum facility in the Penn Quarter neighborhood was built by Milton Maltz and The House on F Street, L.L.C. at a cost of approximately US$40 million. It opened to the public in 2002. 
 The foundation cost of the original museum was half funded by the Malrite Company; the other $20 million came from the District of Columbia through enterprise zone bonds and TIF bonds. The museum was part of the ongoing rejuvenation of Penn Quarter, kicked off in the 1980s by the Pennsylvania Avenue Development Corporation.
 In April 2015, plans were released for a new museum designed by Rogers Stirk Harbour + Partners. In January 2019, the museum began the process of moving from its previous F Street location to the new $162 million dedicated building at 700 L'Enfant Plaza, and it reopened to the public on May 12, 2019. The 32,000 square foot L'Enfant Plaza building has a 145-seat theater, rooftop terrace, and top-floor event space. The new museum is a non-profit enterprise.
 Educational and cultural programs are offered for students, adults, and families including scholarly lectures, films, book signings, hands-on workshops, and group tour packages. The museum charges admission fees.
 The museum houses more than 7,000 artifacts with around 1,000 on public display, accompanied by historical photographs, interactive displays, film, and video. The permanent collection traces the complete history of espionage, from the Ancient Greeks and the Roman Empire, the Middle Ages, the Renaissance, the British Empire, the American Revolutionary War, the French Revolution, the Napoleonic Wars, the American Civil War, both World Wars, the Cold War, and through present day espionage activity. Exhibits include:
 Visitors receive an Undercover Mission badge and cover identity in the "Briefing Center". Here, visitors preview the museum's spy artifacts and watch a five-minute film introducing the shadow world of spying.
 In the "Stealing Secrets" gallery, visitors learn about spies and spymasters, gadget makers, scientists, and engineers from past and present. Hundreds of imaginative inventions used to steal secrets are displayed in this gallery.
 In the "Making Sense of Secrets" gallery, visitors learn how secret information gets turned into useful intelligence. The gallery's interactive exhibits inform how codes are made, analyzed, and broken.
 In the "Covert Action" gallery, visitors discover the age-old techniques leaders use to secretly influence events abroad. They learn about covert mission failures and successes from sabotage to lethal action.
 The "Spying that Shaped History" gallery illustrates the impact of intelligence on history. Visitors explore stories from the American Revolution to 21st century cyberwarfare and hear what intelligence officers think about on-screen spies.
 The "An Uncertain World" gallery explores how spy agencies protect against threats at home. Visitors learn what can happen when they go too far and delve into spy tales from Renaissance Venice to Cold War Berlin. 
 Visitors receive the conclusion to their Undercover Mission in the Debriefing Center including a performance debrief that summarizes their top spy skills.
 The museum had an interactive exhibit called Operation Spy where visitors assumed the roles of covert agents and participated in a one-hour Hollywood-style spy simulation. Visitors moved from area to area, interacting with puzzles, tasks, motion simulators, sound effects, and video messages to work through a mission to intercept a secret arms deal involving a nuclear trigger.
 In 2011, the museum had an interactive called Spy in the City where visitors were given a GPS-type device and had to find clues near various landmarks in the area surrounding the museum to obtain the password for a secret weapon.
 Then-President of the United States, Barack Obama, visited the museum on July 30, 2010.