Complex sentence: In contrast to Polymerase Cycling Assembly, Gibson Assembly is a single-step, isothermal reaction with larger sequence-length capacity; ergo, it is used in place of Polymerase Cycling Assembly for genomes larger than 6 kb.
Split into simpler sentences: In contrast to Polymerase Cycling Assembly, Gibson Assembly is a single-step, isothermal reaction with larger sequence-length capacity. Ergo, it is used in place of Polymerase Cycling Assembly for the synthesis of genomes larger than 6 kb.