Complex sentence: Specifically, minigenes can act as a probe of which factors are important in splicing outcomes: minigenes can be constructed to test the way both cis-regulatory elements (RNA effects) and trans-regulatory elements (associated proteins/splicing factors) affect gene expression.
Split into simpler sentences: Specifically, minigenes can act as a probe of which factors are important in splicing outcomes. Minigenes can be constructed to test the way both cis-regulatory elements (RNA effects) and trans-regulatory elements (associated proteins/splicing factors) affect gene expression.