Complex sentence: The point mutation in the N-terminal sequence results in a salt bridge formation between Glu7 and Lys24 which in turn results in decreased positive electrostatic forces, the net positive charges in Pi2 and Pi3 are 7 and 6 respectively.
Split into simpler sentences: The point mutation in the N-terminal sequence results in a salt bridge formation between Glu7 and Lys24 which in turn results in decreased positive electrostatic forces. The net positive charges in Pi2 and Pi3 are 7 and 6 respectively.