Complex sentence: Rational protein design approaches make predictions of protein sequences that will fold to specific structures which can later be tested experimentally through methods such as peptide synthesis, site-directed mutagenesis or artificial gene synthesis.
Split into simpler sentences: Rational protein design approaches make predictions of protein sequences that will fold to specific structures. These predicted sequences can then be validated experimentally through methods such as peptide synthesis, site-directed mutagenesis or artificial gene synthesis.