Complex sentence: Differential binding is carried out by the induced fit mechanism-the substrate first binds weakly, then the enzyme changes conformation increasing the affinity to the transition state and stabilising it, so reducing the activation energy to reach it.
Split into simpler sentences: Differential binding is carried out by the induced fit mechanism. The substrate first binds weakly, then the enzyme changes conformation increasing the affinity to the transition state and stabilising it, so reducing the activation energy to reach it.