{{위키데이터 속성 추적}}
[[파일:MiR-200 secondary structure.png|220px|섬네일|오른쪽|miR-200 마이크로RNA의 이차구조]]
분자 생물학에서 '''mir-200 mircoRNA'''는 짧은 RNA분자이다. [[마이크로RNA]]는 [[mRNA]]에 붙고 그것이 잘라지게 하는 방법으로 번역을 막아 다른 [[유전자]]의 발현 정도를 조절한다. miR-200과는 miR-200a, miR-200b, miR-200c, miR-141, miR-429를 포함한다. miR-200 마이크로RNA가 [[암]]전이에 연관이 있다는 증거는 속속히 드러나고 있다.<ref name="pmid18411277">{{저널 인용|author=Korpal M|author2=Lee ES|author3=Hu G|author4=Kang Y |title=The miR-200 family inhibits epithelial-mesenchymal transition (EMT) and promotes mesenchymal-epithelial transition (MET) by direct targeting of E-cadherin transcriptional repressors ZEB1 and ZEB2 |journal=J. Biol. Chem. |volume=283 |issue=22 |pages=14910–4 |date=2008-05 |pmid=18411277 |doi=10.1074/jbc.C800074200 |url=}}</ref>

== 게놈 위치 ==
miR-200의 다섯 구성원은 두 가지의 클러스터에 존재한다. 사람에서는 miR-200a, miR-200b, miR-429는 [[염색체]]1번에 위치하고, miR-200c, miR-141은 [[염색체]]12번에 위치한다. 쥐에서는 두 클러스터가 [[염색체]]4번 6번에 위치한다.<ref name="pmid18411277"/>

== 발현 ==
miR-200과의 구성원은 [[상피]]조직에 풍부하다.<ref name="pmid15944708">{{저널 인용|author=Lu J|author2=Getz G|author3=Miska EA|저자표시=et al. |title=MicroRNA expression profiles classify human cancers |journal=Nature |volume=435 |issue=7043 |pages=834–8 |date=2005-06 |pmid=15944708 |doi=10.1038/nature03702 |url=}}</ref>

== 암진행에 연관 ==
miR-200과는 전이의 시작인 상피-간엽 전환(EMT, epithelial-mesenchymal transition)에 중요한 역할을 한다고 알려졌다. EMT는 배아 발생의 한 부분이며, 암의 진행과 많은 유사점을 공유한다. EMT과정에서 세포는 접착능력을 잃고 운동성이 증가한다. 이것은 E-[[캐드헤린]]발현의 억제로 나타나며 전이의 처음 과정이다.

대조적으로, miR-200은 전이의 마지막 단계를 촉진하는 것으로 보였으며 이것은 전이되는 암세포가 EMT의 반대과정인 MET를 전이되어 도착된 세포에서 콜로니를 형성할 때 일어난다. 쥐의 동형 암 세포 라인에서, miR-200과는 전이를 할 수 있는 세포(4T1세포)에서는 강하게 발현되지만, 콜로니를 형성 할 수 없는 세포(4TO7세포)에서는 발현되지 않았다.
비전이성세포인 4TO7세포에서 miR-200c의 과발현은 쉽게 MET를 가능하게 하였으며 간과 폐에 콜로니를 생성하였다.<ref>{{저널 인용| doi = 10.1158/0008-5472.CAN-10-1346 | author = Dykxhoorn DM | year = 2010 | title = MicroRNAs and metastasis: little RNAs go a long way | url = | journal = Cancer Res | volume = 70 | issue = 16| pages = 6401–6406 | pmid = 20663901 | pmc = 2922433 }}</ref>

miR-200은 E-[[캐드헤린]]전사 억제자인 [[ZEB1]]과 [[ZEB2]]를 대상으로 한다. miR-141과 miR200b는 E-[[캐드헤린]]의 발현을 줄여서 세포의 이동능력을 증가시키고 EMT를 유발한다.<ref name="pmid18376396">{{저널 인용|author=Gregory PA|author2=Bert AG|author3=Paterson EL|저자표시=et al. |title=The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1 |journal=Nat. Cell Biol. |volume=10 |issue=5 |pages=593–601 |date=2008-05 |pmid=18376396 |doi=10.1038/ncb1722 |url=}}</ref><ref name="pmid18381893">{{저널 인용|author=Park SM|author2=Gaur AB|author3=Lengyel E|author4=Peter ME |title=The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2 |journal=Genes Dev. |volume=22 |issue=7 |pages=894–907 |date=2008-04 |pmid=18381893 |pmc=2279201 |doi=10.1101/gad.1640608 |url=}}</ref>

=== 암 ===
EMT와 [[암]]진행에 있어서 miR-200의 역할은 연결되어있다:

* [[방광암]]<ref name="pmid19671845">{{저널 인용|author=Adam L|author2=Zhong M|author3=Choi W|저자표시=et al. |title=miR-200 expression regulates epithelial-to-mesenchymal transition in bladder cancer cells and reverses resistance to epidermal growth factor receptor therapy |journal=Clin. Cancer Res. |volume=15 |issue=16 |pages=5060–72 |date=2009-08 |pmid=19671845 |doi=10.1158/1078-0432.CCR-08-2245 |url=}}</ref><ref name="pmid20473948">{{저널 인용|author=Wiklund ED|author2=Bramsen JB|author3=Hulf T|저자표시=et al. |title=Coordinated epigenetic repression of the miR-200 family and miR-205 in invasive bladder cancer |journal=Int J Cancer |volume= 128|issue= 6|pages= 1327–34|date=2010-05 |pmid=20473948 |doi=10.1002/ijc.25461 |url=}}</ref>
* [[유방암]]<ref name="pmid19839049">{{저널 인용|author=Tryndyak VP|author2=Beland FA|author3=Pogribny IP |title=E-cadherin transcriptional down-regulation by epigenetic and microRNA-200 family alterations is related to mesenchymal and drug-resistant phenotypes in human breast cancer cells |journal=Int. J. Cancer |volume=126 |issue=11 |pages=2575–83 |date=2010-07 |pmid=19839049 |doi=10.1002/ijc.24972 |url=}}</ref><ref name="pmid19787069">{{저널 인용|author=Dykxhoorn DM|author2=Wu Y|author3=Xie H|저자표시=et al. |editor1-last=Blagosklonny |editor1-first=Mikhail V. |title=miR-200 enhances mouse breast cancer cell colonization to form distant metastases |journal=PLoS ONE |volume=4 |issue=9 |pages=e7181 |year=2009 |pmid=19787069 |pmc=2749331 |doi=10.1371/journal.pone.0007181 |url=}}</ref>
* [[흑색종]]<ref name="pmid20957176">{{저널 인용|author=Elson-Schwab I|author2=Lorentzen A|author3=Marshall CJ |editor1-last=Danen |editor1-first=Erik H. J. |title=MicroRNA-200 family members differentially regulate morphological plasticity and mode of melanoma cell invasion |journal=PLoS ONE |volume=5 |issue=10 |pages= e13176|year=2010 |pmid=20957176 |pmc=2949394 |doi=10.1371/journal.pone.0013176 |url=}}</ref>
* [[난소암]]<ref name="pmid19501389">{{저널 인용|author=Hu X|author2=Macdonald DM|author3=Huettner PC|author4=Feng Z|author5=El Naqa IM|author6=Schwarz JK|author7=Mutch DG|author8=Grigsby PW|author9=Powell SN|author10=Wang X |title=A miR-200 microRNA cluster as prognostic marker in advanced ovarian cancer. |journal=Gynecol Oncol |volume=114 |issue=3 |pages=457–64 |year=2009 |pmid=19501389 |doi=10.1016/j.ygyno.2009.05.022}}</ref>
* [[췌장암]]<ref name="pmid20388782">{{저널 인용|author=Ali S|author2=Ahmad A|author3=Banerjee S|author4=Padhye S|author5=Dominiak K|author6=Schaffert JM|author7=Wang Z|author8=Philip PA|author9=Sarkar FH |title=Gemcitabine sensitivity can be induced in pancreatic cancer cells through modulation of miR-200 and miR-21 expression by curcumin or its analogue CDF. |journal=Cancer Res |volume=70 |issue=9 |pages=3606–17 |year=2010 |pmid=20388782 |pmc=2978024 |doi=10.1158/0008-5472.CAN-09-4598}}</ref><ref name="pmid19654291">{{저널 인용|author=Li Y|author2=VandenBoom TG|author3=Kong D|author4=Wang Z|author5=Ali S|author6=Philip PA|author7=Sarkar FH |title=Up-regulation of miR-200 and let-7 by natural agents leads to the reversal of epithelial-to-mesenchymal transition in gemcitabine-resistant pancreatic cancer cells. |journal=Cancer Res |volume=69 |issue=16 |pages=6704–12 |year=2009 |pmid=19654291 |doi=10.1158/0008-5472.CAN-09-1298 |pmc=2727571}}</ref>
* [[전립선암]]<ref name="pmid19544444">{{저널 인용|author=Kong D|author2=Li Y|author3=Wang Z|author4=Banerjee S|author5=Ahmad A|author6=Kim HR|author7=Sarkar FH |title=miR-200 regulates PDGF-D-mediated epithelial-mesenchymal transition, adhesion, and invasion of prostate cancer cells. |journal=Stem Cells |volume=27 |issue=8 |pages=1712–21 |year=2009 |pmid=19544444 |doi=10.1002/stem.101}}</ref>
* [[위암]]<ref name="pmid20484038">{{저널 인용|author=Shinozaki A|author2=Sakatani T|author3=Ushiku T|author4=Hino R|author5=Isogai M|author6=Ishikawa S|author7=Uozaki H|author8=Takada K|author9=Fukayama M |title=Downregulation of microRNA-200 in EBV-associated gastric carcinoma. |journal=Cancer Res |volume=70 |issue=11 |pages=4719–27 |year=2010 |pmid=20484038 |doi=10.1158/0008-5472.CAN-09-4620}}</ref>
* [[폐암]]<ref name="pmid19759262">{{저널 인용|author=Gibbons DL|author2=Lin W|author3=Creighton CJ|author4=Rizvi ZH|author5=Gregory PA|author6=Goodall GJ|author7=Thilaganathan N|author8=Du L|author9=Zhang Y|author10=Pertsemlidis A|author11=Kurie JM |title=Contextual extracellular cues promote tumor cell EMT and metastasis by regulating miR-200 family expression. |journal=Genes Dev |volume=23 |issue=18 |pages=2140–51 |year=2009 |pmid=19759262 |pmc=2751985 |doi=10.1101/gad.1820209}}</ref>

== 각주 ==
{{각주}}

== 참고 문헌 ==
<div style='display:none'>
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</div>

[[분류:생물학]]