Patent SG-11201809381X-A (SG)
Title: Polynucleotides encoding interleukin-12 (il12) and uses thereof
Inventor: FREDERICK JOSHUA
IPC: C12N15/67
Filed: 2017-05-18
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 23 November 2017 (23.11.2017) WIPO I PCT omit VIII two III VIII NEE 111°011mm oimIE (10) International Publication Number WO 2017/201350 Al (51) International Patent Classification: A61K 38/20 (2006.01) Cl 2N 15/67 (2006.01) CO7K 14/54 (2006.01) (21) International Application Number: PCT/US2017/033422 (22) International Filing Date: 18 May 2017 (18.05.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/338,483 18 May 2016 (18.05.2016) US 62/443,693 07 January 2017 (07.01.2017) US SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) 1-1 kr) O 1-1 O N _ N O 1-1 N C (71) Applicant: MODERNATX, INC. [US/US]; 200 Technol- with sequence listing part of description (Rule 5.2(a)) ogy Square, Cambridge, Massachusetts 02139 (US). (72) Inventors: FREDERICK, Joshua; c/o MODERNATX, INC., 200 Technology Square, Cambridge, Massachu- setts 02139 (US). HEWITT, Susannah; c/o MODER- NATX, INC., 200 Technology Square, Cambridge, Mass- achusetts 02139 (US). BAI, Ailin; c/o MODERNATX, INC., 200 Technology Square, Cambridge, Massachu- setts 02139 (US). HOGE, Stephen; c/o MODERNATX, INC., 200 Technology Square, Cambridge, Massachusetts 02139 (US). PRESNYAK, Vladimir; c/o MODERNATX, INC., 200 Technology Square, Cambridge, Massachusetts 02139 (US). MCFADYEN, Iain; c/o MODERNATX, INC., 200 Technology Square, Cambridge, Massachusetts 02139 (US). BENENATO, Kerry; c/o MODERNATX, INC., 200 Technology Square, Cambridge, Massachusetts 02139 (US). KUMARASINGHE, Ellalahewage Sathya- jith; c/o MODERNATX, INC., 200 Technology Square, Cambridge, Massachusetts 02139 (US). (74) Agent: MANDRAGOURAS, Amy, E. et al.; Nelson Mullins Riley & Scarborough LLP, One Post Office Square, Boston, Massachusetts 02109-2127 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (54) Title: POLYNUCLEOTIDES ENCODING INTERLEUKIN-12 (IL12) AND USES THEREOF (57) : The present disclosure relates to polynucleotides comprising an open reading frame of linked nucleosides encoding human interleukin-12 (IL12), functional fragments thereof, and fusion proteins comprising IL12. In some embodiments, the open read- ing frame is sequence-optimized. In particular embodiments, the disclosure provides sequence-optimized polynucleotides comprising nucleotides encoding the polypeptide sequence of human IL12, or sequences having high sequence identity with those sequence opti- mized polynucleotides.