Patent SG-11201811783Y-A (SG)
Title: Functionalised chromatography medium comprising polymer nanofibres and process of preparation thereof
Inventor: HUMMERSONE MARC
IPC: B01J20/28
Filed: 2017-07-14
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111111111111111111111111111111111111111111111111111111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2018/011600 Al 18 January 2018 (18.01.2018) W I P0 I P C T (51) International Patent Classification: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, BO1J 20/28 (2006.01) BO1J 20/32 (2006.01) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. BO1J 20/286 (2006.01) (84) Designated States (unless otherwise indicated, for every (21) International Application Number: kind of regional protection available): ARIPO (BW, GH, PCT/GB2017/052085 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (22) International Filing Date: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 14 July 2017 (14.07.2017) TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (25) Filing Language: English MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (26) Publication Language: English TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (30) Priority Data: 1612249.1 14 July 2016 (14.07.2016) GB Published: 1612252.5 14 July 2016 (14.07.2016) GB — with international search report (Art. 21(3)) 1709996.1 22 June 2017 (22.06.2017) GB (71) Applicant: PURIDIFY LTD. [GB/GB]; Stevenage Bio- science Catalyst, Gunnels Wood Road, Stevenage Hertford- shire SG1 2FX (GB). (72) Inventors: HUMMERSONE, Marc; 19 Town Lane, Ben- _ ington, Stevenage, Hertfordshire, SG2 7LA (GB). WAL- LIS, Benjamin; 30 Abbots Grove, Stevenage, Hertford- = shire, SG1 1NR (GB). LEROUX, Frederic; Flat 7, 65-67 Torianno Avenue, Kentish Town, Greater London NW5 = _ 2SG (GB). LAW, William; Ivy Cottage, 77 Chishill Road, = Heydon, Cambridgeshire, SG8 8PN (GB). Agent: NEWMAN, Alastair James Morley; J A Kemp, 14 South Square, Gray's Inn, London, Greater London WC1R = (74) = 5JJ (GB). Designated States (unless otherwise indicated, for every (81) = — kind of national protection available): AE, AG, AL, AM, — AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, = = DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, _ HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, = KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, = OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, = = = (54) Title: FUNCTIONALISED CHROMATOGRAPHY MEDIUM COMPRISING POLYMER NANOFIBRES AND PROCESS OF THEREOF PREPARATION _ (57) : The present invention provides a functionalised polymeric chro- 1-1 R i matography medium, prepared by a process which comprises (i) providing a sub- O OH 7-7---- '0H strafe formed of one or more polymer nanofibres, (ii) grafting one or more neutral © R 2 (I) 0 (II) polymer chains from the substrate, and (iii) contacting the grafted product with a 1-1 R R 4 R5 reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its 1-1 enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastere- 0 --.... omers thereof: with one or more functional groups present on the nanofibre substrate, 00 wherein R 1, R 2, R R and R 3, 4 5 may be the same or different, and are chosen from 1-1 0 H, halogen, C 1 -C 4 alkyl, or C i-C 4 alkoxy provided that at least one of R 1, R 2, ei R 3, R 4 or R 5 is not hydrogen. O