Patent SG-11201908785S-A (SG)
Title: Methods and kits for determining binding sites
Inventor: PILETSKY SERGEY
IPC: G01N33/68
Filed: 2018-03-19
Abstract: FigEpitope 2 (6) Antigen (2) MiP nanoparticle 1 (8) 14 2 AU AU Mass Spectrometry m/z 14 Wash off unbound peptides MIP nanoparticle 2 (10) 1-1 GC N 1-1 00 O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 04 October 2018 (04.10.2018) W I PO I PCT D IV o Ho minim io imio VII IE (10) International Publication Number WO 2018/178629 Al (51) International Patent Classification: GO1N 33/68 (2006.01) (21) International Application Number: PCT/GB2018/050707 (22) International Filing Date: 19 March 2018 (19.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1704823.2 27 March 2017 (27.03.2017) GB (71) Applicant: UNIVERSITY OF LEICESTER [GB/GB]; Fielding Johnson Building, Leicester, Leicestershire LE1 7RH (GB). (72) Inventors: PILETSKY, Sergey; c/o University of Leices- ter, Fielding Johnson Building, Leicester, Leicestershire LE1 7RH (GB). PILETSKA, Elena; c/o University of Leicester, Fielding Johnson Building, Leicester, Leicester- shire LE1 7RH (GB). CANFAROTTA, Francesco; c/o University of Leicester, Fielding Johnson Building, Leices- ter, Leicestershire LE1 7RH (GB). JONES, Don; c/o Uni- versity of Leicester, Fileding Johnson Building, Leicester, Leicestershire LE1 7RH (GB). (74) Agent: HUTTER, Anton; Venner Shipley LLP, The Sur- rey Technology Centre, The Surrey Research Park, 40 Oc- cam Road, Guildford, Surrey GU2 7YG (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (54) Title: METHODS AND KITS FOR DETERMINING BINDING SITES (57) : The invention provides methods, compositions, and kits for the characterisation and analysis of proteins. Methods are provided for determining, on a protein, a binding site for a binding partner, the methods comprising: contacting a protein with a plurality of monomers, and polymerising the monomers to create a protein:polymer complex; digesting the protein in the complex to produce a peptide:polymer complex; isolating the peptide:polymer complex; and sequencing the peptide, wherein the peptide corresponds to a binding site for a binding partner. [Continued on next page] WO 2018/178629 Al MIDEDIMOMMIDIMMUMMOMOIDEEEMOMMOVOIS TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))