Patent EA-004060-B1 (EA)
Title: Purified aminocyclopropan-1-carboxylate-synthase and aminocyclopropan-1-carboxylate-oxydase of a coffee plant, coded nucleic acids thereof, methods for transforming a coffee plant cell and transformed coffee plant cells
Inventor: STILES JOHN I
IPC: C12N15/11
Filed: 1997-08-11
Abstract: 1. A substantially pure aminocyclopropan-1-carboxylate (ACC)-synthase from Coffee arabica consisting essentially of the amino acid sequence SEQ. ID. NO.10, Fig. 1. 2. An isolated nucleic acid sequence that codes on expression for an ACC synthase, as claimed in claim 1 comprising nucleotide sequence SEQ. ID. NO.11, Fig. 2, and DNA variants produced on the basis of degeneracy of genetic code. 3. A substantially pure ACC oxidase from Coffee arabica consisting essentially of the amino acid sequence SEQ. ID. NO.12, Fig. 3. 4. An isolated nucleic acid sequence that codes an expression for Coffee arabica ACC oxidase, as claimed in claim 3 comprising SEQ. ID. No. 13, Fig. 4, and DNA variants received on the basis of degeneracy of genetic code. 5. A transforming vector comprising a transcription promoter of a coffee plant cell operably linked to a nucleic acid sequence, as claimed in claim 2. 6. The transforming vector of claim 5, wherein the nucleic acid sequence is operably linked to the transcription promoter in a sense orientation. 7. The transforming vector of claim 5, wherein the nucleic acid sequence is operably linked to the transcription promoter in an antisense orientation. 8. The transforming vector comprising a transcription promoter of a coffee plant cell operably linked to the nucleic acid sequence, as claimed in claim 4. 9. The transforming vector of claim 8, wherein the nucleic acid sequence is operably linked to the transcription promoter in a sense orientation. 10. The transforming vector of claim 8, wherein the nucleic acid sequence is operably linked to the transcription promoter in an antisense orientation. 11. A transformed coffee plant cell produced by the process of inserting a transforming vector into a coffee plant cell, as claimed in any of claims 5-7. 12. The transformed coffee plant cell of claim 11, wherein said vector is a vector of claim 6. 13. The transformed coffee plant cell of claim 11, wherein said vector is a vector of claim 7. 14. The transformed coffee plant cell produced by the process of inserting a transforming vector into a coffee plant cell, as claimed in any of claims 8-10. 15. The transformed coffee plant cell of claim 11, wherein said vector is a vector of claim 9. 16. The transformed coffee plant cell of claim 11, wherein said vector is a vector of claim 10. 17. A coffee plant cell transformed with a nucleic acid sequence of claim 2 and DNA sequence of claim 4 by the process of inserting a transforming vector of claim 5 and transforming vector of claim 8 into a coffee plant cell. 18. The transformed coffee plant cell of claim 17, wherein at least one of nucleic acid sequence is linked to a transcription promoter in an antisense orientation. 19. A method for transforming a coffee plant cell with a nucleic acid sequence as claimed in claim 2 comprising providing a transforming vector of claim 7, wherein the nucleic acid sequence is operably linked to a transcription promoter in an antisense orientation; and inserting the transforming vector into a coffee plant cell. 20. The method for transforming a coffee plant cell with a nucleic acid sequence as claimed in claim 4 comprising providing a transforming vector of claim 10, wherein the nucleic acid sequence is operably linked to a transcription promoter in an antisense orientation; and inserting the transforming vector into a coffee plant cell. 21. The method for transforming a coffee plant cell with a nucleic acid sequence of claim 2 and a nucleic acid sequence of claim 4 comprising providing first transforming vector as claimed in claim 5, providing second transforming vector as claimed in claim 8, wherein at least one of the above mentioned DNA sequence is operably linked to a transcription promoter in an antisense orientation; and inserting each of the transforming vectors into a coffee plant cell. 22. The method for transforming a coffee plant cell with a nucleic acid sequence of claim 2 comprising providing a transforming vector as claimed in claim 6 and inserting the transforming vector into a coffee plant cell. 23. The method for transforming a coffee plant cell with a nucleic acid sequence of claim 4 comprising providing a transforming vector as claimed in claim 9 and inserting the transforming vector into a coffee plant cell. 24. The method for transforming a coffee plant cell with a nucleic acid sequence of claim 2 and a nucleic acid sequence of claim 4 comprising providing first transforming vector as claimed in claim 5, providing second transforming vector as claimed in claim 8, wherein at least one of the above mentioned DNA sequences is operably linked to a transcription promoter in a sense orientation; and inserting the transforming vector into a coffee plant cell.