Patent EA-000634-B1 (EA)
Title: Recombinant doudle-stranded dna molecule sequence for expression sucrophosphorylase in a plant, method of producing a transgenic plant with increased expression of sucrophosphorylase in different parts of a plant and a line of transformed plant cells
Inventor: BARRY GERARD FRANCIS
IPC: C12N15/29
Filed: 1996-02-08
Abstract: 1. A recombinant, double-stranded DNA molecule comprising in sequence for encoding sucrose phosphorylase comprising constructive elements: a promoter, active in cells of target plant tissue, a gene encoding sucrose phosphorylase enzyme Streptococcus mutans having the sequence of nucleatides:   a 3' non-translated DNA sequence which functions in plant cells to cause transcriptional termination of sucrosephosphorylase gene and the addition of polyadenylated RNA sucrosephosphorylase. 2. A recombinant, double-stranded DNA molecule wherein said promoter is selected from the group consisting of a zein promoter, a patatin promoter, a rice glutein promoter, the subunit soybean 7S promoter, a promoter of ADP glucose pyrophosphorylase, the TFM7 promoter, and the tomato TFM9 promoter. 3. The method of producing a transgenic plant, with increased expression of sucrose phosphorylase enzyme, comprising the steps of inserting into the genome of a plant cell non-differentiated callus of a recombinant, double-stranded DNA molecule according to Claim 1 or 2 and regenerating from said transformed plant cells a genetically transformed plant, the genome of which contains said DNA sequence. 4. The method of Claim 3 wherein increased polysaccharide (e.g. starch) content in solids of a transformed plant and more uniform distribution of starch and solids of said transformed plant. 5. The method of Claim 4 wherein said genetically transformed plant is selected from the group consisting of maize, wheat, rice, tomato, potato, sweet potato, peanut, barley, cotton, strawberry, raspberry, and cassava. 6. The method of Claim 3 wherein increased expression of sucrose phosphorylase enzyme increases starch content in solids of said transformed plant. 7. The method of Claim 4, wherein said genetically transformed plant is selected from the group consisting of potato, sweet potato and tomato. 8. The method of Claim 3 wherein a recombinant, double-stranded DNA molecule is introduced into genome of a potato plant cell, a maize plant cell, a rice plant cell, a wheat plant cell, a tomato plant cell, a barley plant cell, a sugarbeet plant cell, a sweetpotato plant cell, a sugarcane plant cell, a banana plant cell, a plantain plant cell. 9. The method of Claim 8 wherein said plant cell is a potato plant cell. 10. The method of Claim 8 wherein said plant cell is a maize plant cell. 11. The method of Claim 8 wherein said plant cell is a rice plant cell, a wheat plant cell, a tomato plant cell, a barley plant cell. 12. A line of transformed plant cells obtained according to Claims 3-11 comprising a recombinant, double-stranded DNA molecule according to any of 1-2 Claim. 13. A line according to Claim 12 wherein said cells are a potato plant cell, a maize plant cell, a rice plant cell, a wheat plant cell, a tomato plant cell, a barley plant cell, a sugarbeet plant cell, a sweetpotato plant cell, a sugarcane plant cell, a banana plant cell, a plantain plant cell and a strawberry plant cell.