Patent HU-0202250-A2 (HU)
Title: Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
Inventor: HABERHAUER GREGOR
IPC: C12N15/31
Filed: 2000-06-23
Abstract: Isolated Corynebacteium glutamicumnucleic acids encoding membrane construction and membrane transport (MCT) proteins or their portions, are new. Isolated Corynebacteium glutamicumnucleic acids encoding membrane construction and membrane transport (MCT) proteins or their portions, are new. A MCT nucleic acid (N1) does not consist of any of the F-designated genes defined in the specification and is selected from: (a) one of the 338 nucleic acid sequences (S1) defined in the specification, or their portions; (b) a nucleic acid which encodes a polypeptide selected from one of the 338 polypeptide sequences (S2) defined in the specification; or (c) a nucleic acid which encodes a naturally occurring allelic variant of a polypeptide selected from S2. Independent claims are also included for the following: (1) a nucleic acid (N2) comprising a sequence which is at least 50% homologous to a sequence selected from S1 or their portions, provided that the nucleic acid does not consist of any of the F-designated genes defined in the specification; (2) an isolated nucleic acid (N3) comprising a fragment of at least 15 nucleotides of a nucleic acid comprising a sequence selected from S1; (3) an isolated nucleic acid (N4) which hybridizes to N1 to N3 under stringent conditions; (4) a nucleic acid (N5) comprising the sequence of N1, N2, N3 or N4, or their portions, and a nucleic acid sequence encoding a heterologous polypeptide; (5) an expression vector comprising N1, N2, N3, N4 or N5; (6) a host cell transfected with the expression vector of (5); (7) a method of producing a polypeptide, comprising culturing the cell of (6); (8) an isolated Corynebacteium glutamicumMCT polypeptide or its portion; (9) an isolated polypeptide (P1) comprising a sequence selected from S2, provided that the sequence is not encoded by any of the F-designated genes defined in the specification; (10) an isolated polypeptide (P2) comprising a naturally occurring allelic variant of P1; (11) an isolated polypeptide which encoded by N2; (12) an isolated polypeptide comprising a sequence which is at least 50% homologous to a sequence selected from S2, provided that the sequence is not encoded by any of the F-designated genes defined in the specification; (13) a method (M1) of producing a chemical, comprising culturing a cell transfected with the vector of (5) and recovering the chemical from the culture; (14) a method for producing a chemical, comprising culturing a cell whose genomic DNA has been altered by the inclusion of N1, N2, N3, N4 or N5; (15) a method for diagnosing the presence or activity of Corynebacterium diphtheriaein a subject, comprising detecting the presence of one or more of the sequences selected from S1 and S2, providing that the sequences are not or are not encoded by any of the F-designated sequences defined in the specification; and (16) a host cell comprising a nucleic acid selected from S1, where: (a) the nucleic acid is disrupted; (b) the nucleic acid comprises one or more modifications; or (c) the regulatory region of the nucleic acid is modified relative to the wild-type regulatory region.