Patent IL-102615-A (IL)
Title: Immunoassy kit for simultaneously detecting antibodies to hiv-1, hiv-2, htlv-1 and htlv-ii and methods for te detections of antibodies
IPC: G01N33/53
Filed: 1992-07-23
Abstract: An immunoassay kit for simultaneously detecting antibodies to HIV-1, HIV-2, HTLV-I and HTLV-II comprising at least one peptide from each of four group of peptides, coupled to a solid support, the peptides groups having the formulae a-X-b, a-Y-b, a-Z-b and a-W-b, wherein: X is selected from the group consisting of Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Ala Ser (BCH-87c) Lys Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Ala Ser (BCH-87ck) Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg (BCH-132) Lys Lys Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Ala Ser (BCH-266) Lys Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Ala Ser Gly Lys Leu Ile (BCH-408) Lys Lys Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp Asn Ala Ser Gly Lys Leu Ile (BCH-408k) and analogs thereof; Y is selected from the group consisting of Arg Val Thr Ala Ile Glu Lys Tys Leu Gln Asp Gln Ala Arg Leu Asn Ser Trp Gly Cys Ala Phe Arg Gln Val Cys His Thr Thr Val Pro Trp Val Pro Trp Val AdnAsp Ser (BCH-202c) Lys Val Thr Ala Ile Glu Lys Tyr Leu Gln Asp Gln Ala Arg Leu Asn Ser Trp Gly Cys Ala Phe Arg Gln Val Cys His Thr Thr Val Pro Trp Val Asn Asp Ser (BCH-202ck) Lys Lys Val Thr Ala Ile Glu Lys Tyr Leu Gln Asp Gln Ala Arg Leu Asn Ser Trp Gly Cys Ala Phe Arg Gln Val Cys His Thr Thr Val Pro Trp Val Asn Asp Ser (BCH-265) Lys Val Thr Ala Ile Glu Lys Tyr Leu Gln Asp Gln Ala Arg Leu Asn Ser Trp Gly Cys Ala Phe Arg Gln Val Cys His Thr Thr Val Pro Trp Val Asn Asp Ser Ala Phe Arg Gln Val (BCH-417) Lys Val Thr Ala Ile Glu Lys Tyr Leu Gln Asp Gln Ala Arg Leu Asn Ser Trp Gly Cys Ala Phe Arg Gln Val His Thr Thr Val Pro Trp Val Asn Asp Ser Gly Lys His Ile (BCH-422) and analogs thereof; Z is selected from the group consisting of Pro His Ser Asn Leu His Ile Leu Glu Pro Ser Ile Pro Trp Lys Ser Lys Pro Tyr Val Glu Pro Thr Ala Pro Gln Val Leu (BCH-234) Ala Ile Val Ser Pro Ser His Asn Leu Ile Leu Pro Pro Phe Ser Leu Ser Pro Val Pro Thr Leu Gly Ser Arg (BCH-416) and analogs thereof; W is selected from the group consisting of Leu Val His Asp Ser Asp Leu Glu His Val Leu Thr Pro Ser Thr Ser Trp Thr Thr Lys Ile Leu (BCH-219) Thr Ser Glu Pro Thr Gln Pro Pro Pro Thr Ser Pro Pro Leu Leu Val His Asp Ser Asp Leu Glu His Val Leu (BCH-456) Thr Ser Glu Pro Thr Gln Pro Pro Pro Thr Ser Pro Pro Leu Val His Asp Ser Asp Leu Glu His Val Leu (BCH-486) and analogs thereof; a is an amino terminus, one to eight amino acids or a substituent effective to facilitate coupling or to improve the immunogenic or antigenic activity of the peptide; and b is a carboxy terminus, one to eight amino acids or a substituent effective to facilitate coupling or to improve the immunogenic or antigenic activity of the peptide.