Patent SG-11201408473U-A (SG)
Title: Methods for inactivation of viruses and bacteria in cell culture media
Inventor: SHIRATORI MASARU KEN
IPC: C12N5/00
Filed: 2013-06-20
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 27 December 2013 (27.12.2013) WIPOIPCT (10) International Publication Number WO 2013/192395 A1 (51) International Patent Classification: C12N 5/00 (2006.01) (21) International Application Number: PCT/US2013/046756 (22) International Filing Date: 20 June 2013 (20.06.2013) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 61/662,349 20 June 2012 (20.06.2012) US 13/844,051 15 March 2013 (15.03.2013) US (71) Applicant (for all designated States except AL, AT, BA, BE, BG, CH, CN, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IN, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR) : GENENTECH, INC. [US/US]; 1 DNA Way, South San Francisco, CA 94080 (US). (71) Applicant (for AL, AT, BA, BE, BG, CH, CN, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IN, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK. SM, TR only)-. F. HOFFMANN-LA ROCHE AG [CH/CH]; Grenzacherstrasse 124, CH-4070 Basel (CH). (72) Inventors: SHIRATORI, Masaru, Ken; 1 DNA Way, South San Francisco, CA 94080 (US). KISS, Robert, David; 1 DNA Way, South San Francisco, CA 94080 (US). PRASHAD, Hardayal; 1 DNA Way, South San Francisco, CA 94080 (US). IVERSON, Raquel; 1 DNA Way, South San Francisco, CA 94080 (US). BOURRET, Justin; 1 DNA Way, South San Francisco, CA 94080 (US). KIM, Michael; 1 DNA Way, South San Francisco, CA 94080 (US). CHARANIYA, Salim; 1 DNA Way, South San Francisco, CA 94080 (US). (74) Agents: ZHOU, Jie et al.; Morrison & Foerster LLP, 425 Market Street, San Francisco, CA 94105-2482 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, [Continued on next page] (54) Title: METHODS FOR INACTIVATION OF VIRUSES AND BACTERIA IN CELL CULTURE MEDIA [P04] mM(0.1,5.9) 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 [Ca] 1.5 mM(0.1,2.9) 1.5 [Ca] mM(0.1,2.9) 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 [P04] mM(0.1,5.9) (57) Abstract: The invention provides for methods of viral inactiv- ation using high temperature short time (HTST) treatment and ad­ justment of various parameters such that generation of precipitate and depositions of precipitate are reduced and/or minimized. o\ m CJ o\ ©A = Media 3 and Media 4 OB = Media 6 @c = Media 11 ©D = Media 12 ©E = Media 13 © F = Media 8 or Media 9 #G = Media 7 WO 2013/192395 A11 lllll llllllll II llllll III lllll lllll lllll III III III lllll lllll lllll llll llll lllll lllllll llll llll OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))