Patent SG-11201811745U-A (SG)
Title: T cell compositions for immunotherapy
Inventor: SLANETZ ALFRED
IPC: C12N5/0786
Filed: 2017-06-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111110111111111011111111 01111101011H011111110011111011101111111011110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\" WO 2018/005712 Al 04 January 2018 (04.01.2018) W I PO I PCT (51) International Patent Classification: (74) Agent: SOMERVILLE, Deborah, A. et al.; Fox Roth- C12N 5/078 (2010.01) CO7K 14/54 (2006.01) schild LLP, Princeton Pike Corporate Center, 997 Lenox C12N 5/0783 (2010.01) CO7K 14/55 (2006.01) Drive, Bldg. 3, Lawrenceville, NJ 08648-2311 (US). C12N 5/0786 (2010.01) A61K 35/17 (2015.01) (81) Designated States (unless otherwise indicated, for every (21) International Application Number: kind of national protection available): AE, AG, AL, AM, PCT/US2017/039846 AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (22) International Filing Date: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, 28 June 2017 (28.06.2017) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (25) Filing Language: English KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (26) Publication Language: English MG, MK, MN, WV, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (30) Priority Data: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 62/355,533 28 June 2016 (28.06.2016) US TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 62/355,506 28 June 2016 (28.06.2016) US (84) Designated States (unless otherwise indicated, for every 62/355,458 28 June 2016 (28.06.2016) US kind of regional protection available): ARIPO (BW, GH, (71) Applicant: GENEIUS BIOTECHNOLOGY, INC. GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, [US/US]; 12 Michigan Drive, Natick, MA 01760 (US). UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (72) Inventors: SLANETZ, Alfred, E.; 14 Nichols Road, Co- EE, ES, FL FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, hasset, MA 02025 (US). NAKAGAWA, Terry, Y.; 3359 — MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, 5M, = Church Street, Evanston, IL 60203 (US). HERRMAN, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Marissa, A.; 1118 Saratoga Street, Apt. 2, Boston, MA KM, ML, MR, NE, 5N, TD, TG). 02128 (US). = (54) Title: T CELL COMPOSITIONS FOR IMMUNOTHERAPY = = = 10 5 cD 104 Pentamer+ 1.10 10 6 105 crs CD107a+ Gamma+ 2 22 -- :\"V 107a+ 0.46 10 6 10 5 03 TNFa 0 25 TNFa IL-2 0.059 E = Antigen 2 6 - 104 '' 104 = C D 103 _ ci_ — 0 = - 103 E (-) 10 0 3 -10 IFNg+ 1.82 1 — 0 .104 IL-2 0.14 0 10 2 10 3 10 4 10 5 = = Comp-BL1-H :: CD197-Alexa = Fluor 48 — 109-Control = CD8+ _ 127395 10 3 0 10 3 IFNy C5 109 fcs 10 4 10 5 -10 3 0 10 3 10 4 10 5 IL-2 C5 109 fcs 10 6 1111111111.11•1 10 6 = 1 0 Pentamer+ 10 5 CD10 7 a+ 62 CI Gamra+ 1 07 a+ 10 5 INF - NFa+ IL -2+ 21.1 E 104 +Antigen 1° m 4 1 -- 10 cz, v3 4 u- 1 o = 1- -. 11 a) 0 10 3 0- -10 3 c-) 0 103 -103 IFN g 10 4 0 -104 -+ IL2 3.5 ei 1-1 10 1 IN ir) Comp-BL1-H 0 --.... 00 1-1 0 (57) : The invention relates ei antigens that are useful for adoptive immunotherapy and methods for making the T cell compositions. C 10 2 10 3 10 4 10 5 :: CD197-Alexa Fluor 48 to compositions comprising -10 3 0 10 3 10 10 5 IFN 7 a heterogeneous population FIG. 6C -10 3 0 10 10 4 105 IL-2 of T cells with reactivity to selected [Continued on next page] WO 2018/005712 Al D ill Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event amendments (Rule 48.2(h)) of receipt of