Patent SG-11201908272U-A (SG)
Title: Methods and microorganisms for making 1,4-butanediol and derivatives thereof from c1 carbons
Inventor: ZHAO XINHUA
IPC: C07K14/195
Filed: 2018-03-13
Abstract: 4-BDO N acre Isocitrate a-ketoglutarateMn a-ketoglutarate dehydrogenase Succinyl COA aS Succinate ----- xaloacetate malyl-CoA ® do\" L-malate Fumarate ........ fumarate ''' 'reauctase (Frd) (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 20 September 2018 (20.09.2018) WIP0 I PCT Iiiimmolimionotiolomomonoimolionflommill (10) International Publication Number WO 2018/169972 Al (51) International Patent Classification: C07K 14/195 (2006.01) C12N 11/14 (2006.01) (21) International Application Number: PCT/US2018/022204 (22) International Filing Date: 13 March 2018 (13.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/470,953 14 March 2017 (14.03.2017) US (71) Applicant: INTREXON CORPORATION [US/US]; 1750 Kraft Drive, Suite 1400, Blacksburg, VA 24060 (US). (72) Inventors: ZHAO, Xinhua; 2771 Alliston Loop, Dublin, CA 94568 (US). HUYNH, Tina; 3301 High Street, Oak- land, CA 94619 (US). ORTH, Jeffrey; 1704 Central Ave., Apt. 6, Alameda, CA 94501 (US). CHAO, Lily Yuin; 72 Langton Street, Unit 3, San Francisco, CA 94103 (US). KEALEY, James; 945 Midpine Way, Sebastopol, CA 95472 (US). (74) Agent: YAO, Gene, J. et al.; Barnes & Thornburg LLP, 1000 N. West St., Suite 1500, Wilmington, DE 19801-1050 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: METHODS AND MICROORGANISMS FOR MAKING 1,4-BUTANEDIOL AND DERIVATIVES THEREOF FROM Cl CARBONS pMMO/ sMMO MDH Methane Methanol Formaldehyde U aceEF + Pyruvate IpdA Acetyl CoA FIG. 7 Citrate 1 -1 (57) : Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as O 1,4-BDO are disclosed. For example, genetically modified methanotrophs that are capable of generating 1,4-BDO at high titers from \" a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also C disclosed. [Continued on next page] WO 2018/169972 Al OII Declarations under Rule 4.17: as to the identity of the inventor (Rule 4.17(i)) as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(ii)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))