Patent SG-11201805214Q-A (SG)
Title: Layered cell culture particles and methods of making thereof
Inventor: PHELPS MWITA
IPC: C12N5/00
Filed: 2016-12-30
Abstract: BaseGranulation Components Solution A Solution B or Suspension Solution C W O 17 / 11755 9 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/117559 Al 6 July 2017 (06.07.2017) WIPO I PCT (51) International Patent Classification: C12N 1/22 (2006.01) C12N 5/00 (2006.01) (21) International Application Number: PCT/US2016/069564 (22) International Filing Date: 30 December 2016 (30.12.2016) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/272,828 30 December 2015 (30.12.2015) US (71) Applicant: LIFE TECHNOLOGIES CORPORATION [US/US]; 5823 Newton Drive, Carlsbad, CA 92008 (US). (72) Inventors: PHELPS, Mwita; 5823 Newton Drive, Carls- bad, CA 92008 (US). GULDE, Paul; 5823 Newton Drive, Carlsbad, CA 92008 (US). FIKE, Richard; 9310 Hunting Valley North, Clarence, NY 14031 (US). REYNOLDS, Mary; 5823 Newton Drive, Carlsbad, CA 92008 (US). HASSETT, Richard; 5823 Newton Drive, Carlsbad, CA 92008 (US). (74) Agent: REDDY, Daphne; 5823 Newton Drive, Carlsbad, CA 92008 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: with international search report (Art. 21(3)) (54) Title: LAYERED CELL CULTURE PARTICLES AND METHODS OF MAKING THEREOF Figure 5b: Cell Culture Media Layered Granules - Formulation 2 (57) : The present invention is directed to dry cell culture media or feeds comprising layered particles. The less stable or sensitive components are separated spatially from reactive components in media, feeds, supplements, or concentrates due to layering. The invention relates to processes for preparing such layered compositions and methods of making cell culture compositions that are stable, thermally, photo-chemically and/or to gamma irradiation. The invention also relates to kits and culture systems using media layered particles. 111111111111110111011111111111010111110111011101011111111111101111111110111111