Patent AU-2006314220-A8 (AU)
Title: Laglidadg homing endonuclease variants having mutations in two functional subdomains and use thereof.
Inventor: PAQUES FREDERIC
IPC: C12N15/79
Filed: 2006-10-03
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date (10) International Publication Number 24 May 2007 (24.05.2007) PCT WO 2007/057781 A3 (51) International Patent Classification: AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CI, CN, C12N 9/22 (2006.01) C12N 15/10 (2006.01) CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, C12N 15/55 (2006.01) C12N 15/79 (2006.01) GB, GD, GE, GI, GM, GT, HN, HR, HU, ID, IL, IN, IS, C12N 15/64 (2006.01) C12N 15/82 (2006.01) JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MD, MG, MK, MN, MW, MX, MY, (21) International Application Number: MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, PCT/IB2006/003893 RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (22) International Filing Date: 25 October 2006 (25.10.2006) (84) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of regional protection available): ARIPO (BW, GI, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, (26) Publication Language: English ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM), European (AT, BE, BG, CI, CY, CZ, DE, DK, EE, ES, FI, (30) Priority Data: FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, NL, PL, PT, PCT/1B2005/003568 RO, SE, SI, SK, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, 25 October 2005 (25.10.2005) IB GN, GQ, GW, ML, MR, NE, SN, TD, TG). - Published: (71) Applicant (for all designated States except US): CEL- with international search report LECTIS [FR/FR]; 102 route de Noisy, F-93235 Cedex Ro- before the expiration of the time limit for amending the mainville (FR). claims and to be republished in the event of receipt of amendments (72) Inventor; and - with sequence listing part of description published sepa(75) Inventor/Applicant (for US only): PAQUES, Frederic rately in electronic form and available upon request from [FR/FR]; 92 Boulevard du Mardchal Joffre, F-92340 the International Bureau Bourg-la-Reine (FR). (88) Date of publication of the international search report: (74) Agent: CABINET ORES; 36 Rue de St P6tersbourg, 4 October 2007 F-75008 Paris (FR). For two-letter codes and other abbreviations, refer to the "Guid (81) Designated States (unless otherwise indicated, for every ance Notes on Codes and Abbreviations" appearing at the begin kind of national protection available): AE, AG, AL, AM, ning of each regular issue of the PCT Gazette. (54) Title: LAGLIDADG HOMING ENDONUCLEASE VARIANTS HAVING MUTATIONS IN TWO FUNCTIONAL SUBDO MAINS AND USE THEREOF. (57) Abstract: A LAGLIDADG homing endonuclease variant, having mutations in two separate subdomains, each binding a dis tinct part of a modified DNA target half-site, said LAGLIDADG homing endonuclease variant being able to cleave a chimeric DNA target sequence comprising the nucleotides bound by each subdomain. Use of said herodimeric meganuclease and derived products for genetic engineering, genome therapy and antiviral therapy.