Patent SG-11201407768Q-A (SG)
Title: Generating pluripotent cells de novo
Inventor: VACANTI CHARLES A
IPC: C12N5/07
Filed: 2013-04-24
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 31 October 2013 (31.10.2013) WIPOIPCT (10) International Publication Number WO 2013/163296 A1 (51) International Patent Classification: C12N 5/07 (2010.01) A61K 48/00 (2006.01) C12N 5/02 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/037996 24 April 2013 (24.04.2013) English (30) Priority Data: 61/637,631 61/779,533 24 April 2012 (24.04.2012) 13 March 2013 (13.03.2013) English US US (71) Applicants: THE BRIGHAM AND WOMEN'S HOS­ PITAL, INC. [US/US]; 75 Francis Street, Boston, Mas­ sachusetts 02115 (US). RIKEN [ JP/JP] ; 2-1, Hirosawa, Wako-Shi, Saitama, 351-0198 (JP). TOKYO WOMEN'S MEDICAL UNIVERSITY [JP/JP]; 8-1, Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 (JP). (72) Inventors: VACANTI, Charles A.; 355 Pond Street, Uxbridge, Massachusetts 01569 (US). VACANTI, Martin P.; 2220 Westchester Drive #6, Manhattan, Kansas 66503 (US). KOJIMA, Koji; 50 Longwood Avenue #414, Brookline, Massachusetts 02446 (US). OBOKATA, Haruko; c/o RIKEN, 2-1, Hirosawa, Wako-shi, Saitama, 351-0198 (JP). WAKAYAMA, Teruhiko; c/o RIKEN, 2- 1, Hirosawa, Wako-shi, Saitama, 351-0198 (JP). SASAI, Yoshiki; c/o RIKEN, 2-1, Hirosawa, Wako-shi, Saitama, 351-0198 (JP). YAMATO, Masayuki; c/o Tokyo Wo­ men's Medical University, 8-1, Kawada-cho, Shinjuku-ku, Tokyo, 162-8666 (JP). (74) Agents: RESNICK, David et al.; Nixon Peabody LLP, 100 Summer Street, Boston, Massachusetts 02110-2131 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, [Continued on next page] (54) Title: GENERATING PLURIPOTENT CELLS DE NOVO 0\ CJ 40%i 35%- 30%- CO LU 25%- CJ + o_ 20%- CD *d- o 15%- c=> 10%- i i r « L T 9 o%- • //// (57) Abstract: The technology de­ scribed herein relates to methods, as­ says, and compositions relating to caus­ ing a cell to assume more pluripotent a state, e.g. without introducing foreign genetic material. ^ i ~—i ~—r J® i-H o CJ o & WO 2013/163296 A11 lllll llllllll II llllll III lllll lllll lllll III III III lllll lllll lllll lllll llll llll lllllll llll llll KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, FT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))