Patent SG-11201804197R-A (SG)
Title: T7 alpha viral vector system
Inventor: WAGNER THOMAS E
IPC: C12N15/86
Filed: 2016-11-18
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property - Organization International Bureau Sd ... ...... ..r .„,\" (43) International Publication Date 26 May 2017(26.05.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111311111111111111111 2017/087763 Publication Al Number (51) International Patent Classification: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, C12N 15/86 (2006.01) Cl 2N 5/074 (2010.01) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, C12N 7/02 (2006.01) C12N 5/071 (2010.01) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (21) International Application Number: OM PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW SA, PCT/US2016/062703 SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (22) International Filing Date: TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, 18 November 2016 (18.11.2016) ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (30) Priority Data: TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, 62/256,788 18 November 2015 (18.11.2015) US TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (71) Applicant: ORBIS HEALTH SOLUTIONS LLC LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, [US/US]; 111 Smith Hines Road, Suites E&F, Greenville, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, South Carolina 29607 (US). GW, KM, ML, MR, NE, SN, TD, TG). (72) Inventor: WAGNER, Thomas E.; 104 Golden Wings Declarations under Rule 4.17: Way, Greenville, South Carolina 29650 (US). — as to applicant's entitlement to apply for and be granted a (74) Agents: SCHORR, Kristel et al.; Foley & Lardner LLP, patent (Rule 4.170) 3000 K Street NW, Suite 600, Washington, District of as to the applicant's entitlement to claim the priority of the Columbia 20007 (US). earlier application (Rule 4.17 (iii)) Designated States indicated, (81) (unless otherwise for every Published: kind of national available): AE, AG, AL, AM, protection AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, — with international search report (Art 21(3)) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, [Continued on next page] Title: T7 ALPHA VIRAL VECTOR SYSTEM (54) Figure 1 A 17 terminator H E FP rigin ‘,. RES , i prom E -' V -- 17 prom r 7/NS P 1- 4/HygEGFP pT , 17.mines: z.gss :h 5 1-1 .41 en IN IN GC ------------------. o nspl -4 IN 1-1 (57) : The present invention relates generally to a gene expression system utilizing an alphavirus replicon and T7 promoter. N The system is capable of expressing proteins in the cell cytoplasm without integrating the gene of interest into the genome of a host cell. The invention has a wide range of applications such as producing induced pluripotent cells and vaccines against pathogens and 0 cancers. WO 2017/087763 Al 111M110111101110111111111111111110111111111111111111111111011111111011110111111 — before the expiration of the time limit for amending the claims and amendments to be (Rule republished 48.2(h)) in the event of receipt of