Patent EA-002605-B1 (EA)
Title: Device and method for obtaining and initially preparing tissue samples for molecular genetic diagnosis
Inventor: BREM GOTTFRIED
IPC: C12Q1/6806
Filed: 1998-05-25
Abstract: 1. A device taking and initially preparing samples containing cells with DNA, comprising: a sample container (1) and means (4) for obtaining the samples, said means being inserted in the sample container after the sample has been obtained and sealed inside said container, wherein  the sample container (1) has a bottom and side walls and is closed with a lid that can be easily perforated and also includes means (5) for fixing the inserted sample obtaining means in an area of the container side walls distant from the bottom, whereby means for protecting against enzymes having a catabolic effect on the DNA are provided in the container, whereby the means for obtaining the sample are embodied in such a way that, once inserted in the sample container, said means are fixed in a stable manner to the means provided in the sample container for fixing which divides the sample container into at least one sample chamber (6,6a) delimited by the bottom (2) and the side walls (3) of the sample container (1) and the front end (7) of the sample obtaining means (4) facing the sample container (1). 2. The device of claim 1,sample container characterized in that the lid that can be easily perforated inwards is a film or water-impermeable membrane. 3. The device of claim 1 or 2, characterized in that the means for protecting against enzymes having a catabolic effect on the DNA is alkali, proteinase K and or a molecular sieve. 4. The device of claim 3, characterized in that the proteinase K is separated from a corresponding buffer by a membrane which is destroyed when the means (4) for obtaining the samples being inserted in the sample container causing proteinase K to contact the buffer. 5. The device of any preceding claim, characterized in that the front end (7) of the sample obtaining means (4) facing the sample container (1) is conical. 6. The device of any preceding claim, characterized in that the front end (7) of the sample obtaining means (4) facing the sample container (1) has at least one sharp edge. 7. The device of any preceding claim, characterized in that the bottom (2) of the sample container (1) is provided with a projection (8) protruding into the inner area of the sample container (1) which, when the means (4) for obtaining the samples being inserted in the sample container, enters into a corresponding recess on the end (7) of the sample obtaining means (4) such that the sample material introduced through the sample obtaining means is crushed between the projection and the sample obtaining means (4). 8. The device of claim 7, characterized in that the projection is connected to the walls of the sample container (1) so that when inserting the sample obtaining means having a corresponding recess into the sample container, said sample container divides into two sample chambers (6,6a). 9. The device of claim 7 or 8, characterized in that the projection (8) is conical. 10. The device of claims 7 to 9, characterized in that the projection (8) is a wall dividing the sample container (1) into two chambers. 11. The device of any preceding claim, characterized in that the sample container (1) is fixed on a holding device. 12. The device of claim 11, characterized in that the holding device is a cover plate. 13. The device of any preceding claim, characterized in that the end of the sample obtaining means (4) opposite the sample container is adapted for a pin. 14. The device of any preceding claim, characterized in that the sample container (1) is fixed to a tenon plate (10) of an ear tag enabling its separation. 15. The device of any preceding claim, characterized in that the sample container (1) is fixed to a mortise plate (10) of an ear tag enabling its separation. 16. The device of claim 14 or 15, characterized in that the ear tag (10,11) and the sample container are provided with similar identification number. 17. A method for obtaining samples containing cells with DNA, characterized in that the sample is obtained using a device as defined in claim 1, wherein the sample is obtained using the end (7) of the sample obtaining means (4), said end (7) facing the sample container (1) and being inserted into the sample container (1), thus forming a sealed sample chamber (6,6a) limited by the bottom (2) and the walls (3) of the sample container (1) and facing the sample container by the end (7) of the sample obtaining means(4). 18. The method of claim 17, characterized in that the sample obtaining means (4) is perforated through an animal ear and introduce into the sample container (1) using a special device. 19. The method of claim 18, characterized in that the animal is simultaneously marked. 20. A method for typing animals population, comprising obtaining animals samples using the device according to any of claims 1 to 16, an analytical treatment of said samples in a laboratory and putting them in a catalogue. 21. A use of the device according to any of claims 1 to 16 for obtaining samples containing cells with DNA.