Patent SG-11201903422W-A (SG)
Title: A detection method
Inventor: CHU JANG HANN
IPC: A61K31/7088
Filed: 2018-03-26
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111111111111111111111111111111111101011101111111111010H0111110111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/174830 Al 27 September 2018 (27.09.2018) W I POI PCT (51) International Patent Classification: crobiology, and Immunology, Science Drive 2, Singapore C12Q 1/6851 (2018.01) A61P 31/14 (2006.01) 117545 (SG). NYO, MM; c/o National University of Sin- A61K 31/7088 (2006.01) gapore, Blk MD4, Level 3, 5, Department of Microbiology, (21) International Application Number: and Immunology, Science Drive 2, Singapore 117545 (SG). PCT/SG2018/050134 (74) Agent: AMICA LAW LLC; 30 Raffles Place, #14-01 (22) International Filing Date: Chevron House, Singapore 048622 (SG). 26 March 2018 (26.03.2018) (81) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (26) Publication Language: English CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (30) Priority Data: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 10201702437X 24 March 2017 (24.03.2017) SG KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (71) Applicants: AGENCY FOR SCIENCE, TECHNOLO- MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, #20-10 Connexis, Singapore 138632 (SG). NATIONAL SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, UNIVERSITY OF SINGAPORE [SG/SG]; 21 Lower TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Kent Ridge Road, Singapore 119077 (SG). (84) Designated States (unless otherwise indicated, for every (72) Inventors: CHU, Jang Hann, Justin; c/o National Univer- kind of regional protection available): ARIPO (BW, GH, sity of Singapore, Blk MD4, Level 3, 5, Department of Mi- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, = = (54) Title: A DETECTION METHOD = 20- — _ 15- = c = = — w = 0- = = .. . -- . ‘ \ k., `N • • LI HFMD Healthy = -5 • • • • • . • • = tit2 f o .Q b 4 2 faQ 0 . 4 2 n a 4 2 42 toQ tiF t 4 1 01' 01 1 4 1 et. N* , i,b 1* eb 41 • 0 •„.;• A T 4 1: A T A „, scr A T Ak •ivr A T A „, •icr ..- i .- , .- .- 74 V :N - _ I • 0 0 0 0 0- 0 0- r y a ry ry a a ry a ry a 0 s‘% co `'‘• `C• `c• Nc• `‘• 1-1 0 Figure 10 M GC 'V (57) : The present invention relates to a method of detecting Hand-Foot-and-Mouth Disease (HMFD) in subjects. In particular, IN the method relates to detecting promising biomarkers in the saliva of these subjects. In an aspect of the present invention, there is 1-1 -- --- GC provided a method for detecting HFMD, the method comprising determining the presence of a microRNA (miRNA) in a saliva sample, wherein the presence of the miRNA in the saliva sample is indicative of HFMD. Preferably, the miRNA is any one selected from the 0 group consisting of mir-18b, mir-125a, mir-145, mir-155, mir-221, mir-324, mir- 335 and further comprises mir-23a and mir-142. ei o [Continued on next page] WO 2018/174830 Al MIDEDIM011010EIREEM311111111111111111111111111111111110111111111111 UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17 (iv)) Published: — with international search report (Art. 21(3)) — in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE