Patent HU-0301136-A2 (HU)
Title: Corynebacterium glutamicum genes encoding proteins involved in carbon metabolism and energy production
Inventor: HABERHAUER GREGOR
IPC: C12N15/31
Filed: 2000-06-23
Abstract: An isolated nucleic acid (I) from Corynebacterium glutamicum encoding a sugar metabolism and oxidative phosphorylation (SMP) protein, or a portion of it, providing that the nucleic acid does not consist of any of the F-designated genes, given in the specification, is new. Independent claims are also included for the following: (1) an isolated C. glutamicum nucleic acid that comprises one of 391 sequences (A), given in the specification, or a portion of it, providing the nucleic acid does not consist of any of the F-designated genes; (2) an isolated nucleic acid which encodes a polypeptide with one of 391 sequences (B), given in the specification, providing the nucleic acid does not consist of any of the F-designated genes; (3) an isolated nucleic acid which encodes a naturally occurring allelic variant of a polypeptide with a sequence of (B), providing that the nucleic acid does not consist of any of the F-designated genes; (4) an isolated nucleic acid comprising a nucleotide which is 50 % homologous to a nucleotide sequence of (A) or a portion of it, providing the nucleic acid does not consist of any of the F-designated genes; (5) an isolated nucleic acid comprising a fragment of 15 nucleotides of a nucleic acid with a sequence of (A), providing the nucleic acid does not consist of any of the F-designated genes; (6) an isolated nucleic acid which hybridizes (I) or (1) - (5) under stringent conditions; (7) an isolated nucleic acid comprising (I) or (1) - (6) or a portion of them and a nucleotide sequence encoding a heterologous polypeptide; (8) a vector comprising (I) or (1) - (7); (9) a host cell transfected with (8); (10) producing a polypeptide comprising culturing (9); (11) an isolated SMP polypeptide from C. glutamicum, or a portion of it; (12) an isolated polypeptide comprising an amino acid sequence of (B), providing that the sequence is not encoded by any of the F-designated genes; (13) an isolated polypeptide which is encoded by a nucleic acid comprising a nucleotide sequence that is 50 % homologous to a sequence of (A), providing that the nucleic acid does not consist of any of the F-designated nucleic acids; (14) an isolated polypeptide comprising an amino acid sequence which is 50 % homologous to a sequence of (B), providing the sequence is not encoded by any of the F-designated genes; (15) producing a fine chemical comprising culturing a cell containing (8); (16) producing a fine chemical comprising culturing a cell whose genomic DNA has been altered by the inclusion of (I) or (1) - (7); (17) diagnosing for the presence or activity of C. diphtheriae in a subject comprising detecting the presence of one of (A) or (B), providing the sequences are not or are not encoded by any of the F-designated sequences; (18) a host cell comprising a nucleic acid of (A), where the nucleic acid has been disrupted; (19) a host cell comprising a nucleic acid of (A), where the nucleic acid comprises a nucleic acid modification; and (20) a host comprising a nucleic acid of (A), where the regulatory region is modified relative to the wild-type regulatory region of the molecule.