baber/WOPTO huggingface dataset:
Patent JP-2022010243-A (JP)
Title: Manufacturing method of storage battery electrode
IPC: H01M4/139
Filed: 2021-11-11
Abstract: PROBLEM TO BE SOLVED: To provide graphene oxide that has high dispersibility and is easily reduced, graphene having high electron conductivity, an electrode for a storage battery having an active material layer having high electric conductivity, a manufacturing method thereof, and a power storage battery having an increased discharge capacity. 
     SOLUTION: A manufacturing method of a power storage battery electrode includes a step of dispersing graphene oxide in a solution containing alcohol or acid, a step of heating the graphene oxide dispersed in the solution, and a step of reducing the graphene oxide. 
     SELECTED DRAWING: Figure 1 
     COPYRIGHT: (C)2022,JPO&INPIT
Patent JP-2022104792-A (JP)
Title: Piperlongumine-based compound and immunoregulator comprising the same
Inventor: JOO SEONG SOO
IPC: C07D413/12
Filed: 2021-11-12
Abstract: To provide a piperlongumine-based compound and an immunoregulator comprising the same.SOLUTION: The invention provides a novel piperlongumine-based compound, an immunoregulator containing as an active ingredient the compound or a pharmaceutically acceptable salt or solvate thereof, and a health functional food for immunoregulation.SELECTED DRAWING: Figure 1
Patent JP-2023072200-A (JP)
Title: game machine
Inventor: NOZAKI TAKU
IPC: A63F7/02
Filed: 2021-11-12
Abstract: To provide a game machine capable of further enhancing interest in a game.SOLUTION: A game machine includes: special condition determination means for determining whether an execution result of a game satisfies a special condition; special game state control means capable of performing control to a special game state capable of granting predetermined game value to a player after completion of a game when it is determined that a special condition is satisfied; game state control means capable of controlling a game state at least including a normal game state, a specific game state more advantageous to a player than the normal game state, and a game-disabled state for disabling a game by a player as a game state in which a game is executed; threshold determination means capable of determining whether the number of times of execution of a special game state or a game value number granted to a player reaches a predetermined threshold; and restriction means capable of controlling a game state controlled by the game state control means to a normal game state or a game-disabled state on the basis of determination by the threshold determination means.SELECTED DRAWING: Figure 330
Patent JP-2023073102-A (JP)
Title: Nozzle, soldering apparatus and method for manufacturing substrate device
Inventor: SHIMIZU NOBUHIRO
IPC: B23K1/00
Filed: 2021-11-15
Abstract: To obtain a nozzle that can suppress the generation of solder bridges compared to the case where the tip of the outlet in the tip member attached to the main body is not stepped with the tip of the opening of the main body.SOLUTION: A nozzle 80 has an attachment 100 with an upper surface 110 that extends outwardly in the radial direction of the outlet 94 from which molten solder J is discharged upwardly and does not project upwardly from the outlet 94, and a lower surface 112 that is connected from the outer edge of the upper surface 110 to the base 80B side and slopes inwardly in the radial direction toward the base 80B side, and a nozzle body 82 which has an aperture 86 at its tip through which molten solder J is discharged, and in which the attachment 100 is attached to the nozzle body with the outlet 94 positioned above the aperture 86.SELECTED DRAWING: Figure 6
Patent JP-2023073613-A (JP)
Title: Lens device, manufacturing method for lens device, and imaging apparatus
Inventor: KIMURA SHOGO
IPC: G02B7/02
Filed: 2021-11-16
Abstract: To provide a lens device advantageous for obtaining high optical performance, for example.SOLUTION: A lens device (100a) includes an optical element (4), a lens barrel (1) housing the optical element (4), a receiving member (2) for receiving the optical element (4), a fixing member (3) for fixing the receiving member (2) to the lens barrel (1), and an adjustment mechanisms (5, 6) capable of adjusting a position of the receiving member (2) in an optical axis direction.SELECTED DRAWING: Figure 1
Patent JP-2022019802-A (JP)
Title: Article recovery device
IPC: B30B9/32
Filed: 2021-11-19
Abstract: PROBLEM TO BE SOLVED: To provide an article recovery device configured to easily detect a foreign objet when it is caught by an outer door, and provide an article collecting device capable of accurately measuring a mass of an article placed on a placing section. 
     SOLUTION: An article recovery device includes: a placing section on which an article can be placed; a measuring section for supporting the placing section; and an outer door that is configured outside a support range of the measuring section and that opens and closes the placing section so as to be exposed. The outer door is configured to move from an open position to a close position toward the placing section. The article recovery device includes a control section that controls movement of the outer door. When moving the outer door from the open position to the close position, the control section controls to stop the movement of the outer door from the open position to the close position upon detection of a change of a measured value by the measuring section. 
     SELECTED DRAWING: Figure 2 
     COPYRIGHT: (C)2022,JPO&INPIT
Patent JP-2023076280-A (JP)
Title: Angular contact ball bearing, bearing unit for dental air turbine, and dental air turbine hand piece
Inventor: OKAZAKI MINETO
IPC: F16C19/36
Filed: 2021-11-22
Abstract: To provide an angular contact ball bearing which makes wear of balls less likely to occur to inhibit reduction of a rotation speed of a dental air turbine over a long time, and to provide a bearing unit for a dental air turbine and a dental air turbine hand piece.SOLUTION: An angular contact ball bearing includes: an outer ring in which an outer ring raceway surface is formed on an inner peripheral surface; an inner ring in which an inner ring raceway surface is formed on an outer peripheral surface; and multiple balls disposed between the outer ring raceway surface and the inner ring raceway surface. The multiple balls include multiple large diameter balls each of which has a contact angle with the outer ring raceway surface and the inner ring raceway surface; and multiple small diameter balls each of which does not have a contact angle with the outer ring raceway surface and the inner ring raceway surface and has a diameter smaller than that of the large diameter ball. The multiple large diameter balls and the multiple small diameter balls are alternately arranged in a circumferential direction and made of the same kind of material and have different colors.SELECTED DRAWING: Figure 2
Patent JP-2023077791-A (JP)
Title: Coupling cuneus for orthogonal pipe of vinyl house
Inventor: SHINOHARA MASATO
IPC: E04B1/58
Filed: 2021-11-25
Abstract: To provide a coupling cuneus for an orthogonal pipe of a vinyl house in which in temporary fastening work, a cuneus is hardly detached from a coupling tool main body by impact of a hammer, temporary fastening of the orthogonal pipes can be achieved by only tightening and pipes can be held.SOLUTION: There is provided a substantially U-shaped cuneus 5 in a cross section which comprises: a plate part 50 which couples two pipes 1, 2 being inserted along insertion guides 40, 41 of a coupling tool main body 4 and being orthogonal; and both side faces 51, 51. For temporary fastening of two pipes 1, 2 held by the coupling tool main body 4 and being orthogonal, the cuneus 5 is inserted along the insertion guides 40, 41 on front and rear ends, then immediately after the cuneus presses and gets over a pipe 2 provided in a notch 42 to which the cuneus is slid and contacts, the cuneus is temporarily engaged and temporarily fastened by a step-shaped temporary fastening salient 6, the step-shaped temporary fastening salient being provided on a surface 50a of the plate part 50.SELECTED DRAWING: Figure 7
Patent JP-2023077853-A (JP)
Title: Residue recovery device and residue recovery method
Inventor: SUGITA KOJI
IPC: B08B3/02
Filed: 2021-11-25
Abstract: To suppress mixing of foreign objects into a residue recovery unit, and to suppress generation of foreign objects from the residue recovery unit.SOLUTION: A residue recovery device 10 includes: a tank 69; an upper cover 12 having a ceiling opening and a carry-in port; a shielding body 14 for shielding the carry-in port; a sink 16 which has a drain port, and whose cross section reduces downward; a filter paper mounting part 35 in which a funnel where a measurement filter paper is mounted has a pouring port connected to the drain port and an outlet; a filtration pump 65 which has a first suction port 65a connected to the outlet, which sucks filtrate from a rear surface of the measurement filter paper and returns it to a tank 69; a washing pump 71 which has a discharge port 71b for discharging a washing liquid 3; a washing valve 75; and a nozzle 79 arranged inside the upper cover 12, connected to the discharge port 71b via the washing valve 75, and having a first filtration filter 79b and a jetting port 79a connected to the downstream of the first filtration filter 79b.SELECTED DRAWING: Figure 2
Patent JP-2022088331-A (JP)
Title: System for manufacturing support structure
Inventor: CEYHAN CELIK
IPC: B29D30/00
Filed: 2021-11-26
Abstract: To provide a system for manufacturing a non-pneumatic wheel/tire assembly with increased performance.SOLUTION: A system 200 cures and manufactures a partially cured tire assembly 140. The system comprises: an annular hub member 210 slid into a corresponding annular, radially inner surface 142; a first annular curing platen which fastens a plurality of elongate spacer members for keeping a corresponding uniform cavity dimension, to the hub member using flap members, thereby keeping the corresponding uniform cavity dimension, which encloses a radially outermost surface of each of the spacer members, and which is for axially securing the hub member and the spacer members relative to each other; a second annular curing platen for axially securing the hub member and the spacer members relative to each other; and a plurality of elongate inserts.SELECTED DRAWING: Figure 1
Patent JP-2023080847-A (JP)
Title: work machine
Inventor: KIYOHARA Daiki
IPC: B25C7/00
Filed: 2021-11-30
Abstract: To improve workability of a work machine.SOLUTION: A driving machine 100 comprises: a coil spring 136 that generates energizing force; a plunger 126, which receives the energizing force generated by the coil spring 136 to move downward in a first direction P; and a drive blade 127 that moves downward in the first direction P together with the plunger 126. Further the driving machine 100 comprises: a housing part 111 having an opening portion on a front side in a second direction Q with respect to the plunger 126; and a cover part 135 covering the opening portion. The cover part 135 is detachably attached to the housing part 111. The driving machine 100 further has a plate 140, which is arranged between the plunger 126 and the drive blade 127 in the second direction Q, and covers at least portions of the plunger 126 and of the coil spring 136.SELECTED DRAWING: Figure 2
Patent JP-2023084759-A (JP)
Title: Radio wave interference monitoring device, and radio wave interference monitoring method
Inventor: SHIOIRI TAKESHI
IPC: G01S3/04
Filed: 2021-12-08
Abstract: To provide a radio wave interference monitoring device and a radio wave interference monitoring method that can efficiently monitor in a short time mutual interference between radio signals at respective places in a space in a complicated radio communication environment in which a plurality of signal sources transmits and receives the radio signals in a same frequency band, and that can be also applied to interference monitoring including a dynamic variation of an interference source.SOLUTION: In a radio wave interference monitoring device 1, a data processing device 40 is communicably connected with receiving sensor units 20 arranged at a plurality of measurement points in a radio wave interference monitoring area, and separates a signal transmitted from any one of a plurality of signal sources from mixed radio signals transmitted from the receiving sensor units 20 and received at the measurement points. A display control unit 55 displays results of analysis of analysis items including electric field intensity, arrival direction estimation, and constellation of the separated signals transmitted from the signal sources, and a result of creation of a heat map on a display 46 as radio wave interference information.SELECTED DRAWING: Figure 4
Patent JP-2022104560-A (JP)
Title: Reception device, reception method and reception program
IPC: G06Q30/06
Filed: 2021-12-14
Abstract: PROBLEM TO BE SOLVED: To provide a reception device, reception method and reception program of setting up a time zone of providing transaction objects at discount prices and receiving order of the transaction objects at the discount prices. 
     SOLUTION: A reception device according to the present disclosure includes an acquisition section that acquires time information representing a time zone providing users with transaction objects at discount prices and price information representing information of a discount price for every transaction object from stores receiving order of transaction objects from the users via a prescribed platform, and a reception section that receives order of the transaction objects based on the price information and time information acquired from the acquisition section. 
     SELECTED DRAWING: Figure 8 
     COPYRIGHT: (C)2022,JPO&INPIT
Patent JP-2022031925-A (JP)
Title: Core component of nuclear reactor and nuclear reactor
IPC: G21C3/33
Filed: 2021-12-14
Abstract: PROBLEM TO BE SOLVED: To provide a nuclear reactor having a structure allowing easy introduction to an existing reactor, and being suppressed in jumping-up of core components for vertical vibration. 
     SOLUTION: A present application is related to a core component of a nuclear reactor supported by a supporting structure including multiple horizontally arranged connection pipes. The core component has a body part, an entrance nozzle and a first pad part. The entrance nozzle is provided at one end side of the body part, and engageable with any one of the multiple connection pipes. The first pad part is provided at least at a part of an outer surface of the other end of the body part, and has a thermal expansion coefficient larger than that of the body part. 
     SELECTED DRAWING: Figure 4 
     COPYRIGHT: (C)2022,JPO&INPIT
Patent JP-2022050438-A (JP)
Title: System and method for supporting router sma abstractions for smp connectivity checks across virtual router ports in high performance computing environment
IPC: H04L41/122
Filed: 2021-12-20
Abstract: PROBLEM TO BE SOLVED: To provide systems and methods for supporting SMP connectivity checks across a virtual router in a high performance computing environment. 
     SOLUTION: An SMA model enhancement method allows for the possibility to send a packet (i.e., SMP) that is addressed to a local router port, and the SMA where the packet is addressed receives the packet, and then applies a new attribute that defines that the requested information is on a remote node (e.g., connected by a physical link across subnets). The SMA operates as a proxy (receiving an SMP and sending another request), or the SMA modifies the original packet and sends it on as an inter-subnet packet. 
     SELECTED DRAWING: Figure 14 
     COPYRIGHT: (C)2022,JPO&INPIT
Patent JP-2023093802-A (JP)
Title: Welding system, welding method, and program
Inventor: WAKITA NAOYA
IPC: B23K9/12
Filed: 2021-12-23
Abstract: To alleviate the burden on a welder.SOLUTION: A welding system controls a welding robot which welds a steel material while moving in a predetermined direction on a guide rail arranged along the steel material. The welding system includes: a position information acquisition section for acquiring erection jig position information related to a position of an erection jig provided on the steel material, and robot position information indicating a position of the welding robot; and a relative information acquisition section for acquiring relative information indicating a position of the welding robot relative to the erection jig, on the basis of the robot position information and the erection jig position information.SELECTED DRAWING: Figure 22
Patent JP-2023095314-A (JP)
Title: Aluminum extruded wire
Inventor: HOSHIKAWA KOSUKE
IPC: C22C21/00
Filed: 2021-12-24
Abstract: To provide an aluminum extruded wire that is made of aluminum having a small amount of alloy element added and has high strength and high strength uniformity in the longitudinal direction.SOLUTION: Provided is an aluminum extruded wire in which Fe, Cu, Ti, Mn, Mg, Cr, B, Ga, V and Zn with the total contained amount of 0.01 mass% or less and one or more selected from the group consisting of Ni, Y and Si are contained, and the balance of which is Al and unavoidable impurities, and in a cross section perpendicular to the longitudinal direction of the extruded wire, both the central measurement area including the center point of the cross section and the peripheral measurement area contacting the outer periphery of the cross section have an average crystal grain size measured by backscattered electron diffraction of 15 to 50 μm, respectively.SELECTED DRAWING: Figure 1B
Patent JP-2023096941-A (JP)
Title: Management system, device, program and management method
Inventor: SATO YUTARO
IPC: G06F3/12
Filed: 2021-12-27
Abstract: To suppress the occurrence of expiration in an encryption key with an expiration date.SOLUTION: A management system is provided with a management apparatus, a device, a storage device, and a server. The storage device and the server are configured to communicate with the management apparatus and the device. When the management apparatus obtains data identification information for identifying execution data used in device execution processing performed by the device from the server, the management apparatus uploads the data identification information to the storage device. When the device downloads the data identification information, the device transmits the data identification information and an encryption key with an expiration date to the server. When the device receives the encrypted execution data from the server, the device decrypts the execution data with the encryption key and uses the execution data to execute the device execution processing.SELECTED DRAWING: Figure 1
Patent JP-2023093280-A (JP)
Title: Rotary type concrete spray machine
Inventor: NISHIO HIDEKAZU
IPC: E04G21/02
Filed: 2021-12-22
Abstract: To provide a rotary type concrete spray machine improving drawbacks such that a discharge rate of the rotary type concrete spray machine used in concrete spraying for a tunnel construction is unstable because of a poor filling rate as material introduced from a hopper is not allowed to easily fall in a chamber, and considerable reduction in the discharge rate makes a large amount of spraying impossible.SOLUTION: A concrete spray machine is provided with: a rotary having a plurality of chambers; a hopper place on an upper part of the rotary; an air chamber arranged on a lower part of the rotary; a rotational shaft rotated through a reduction gear by a motor arranged on an upper part of the hopper and extending in a vertical direction; and a spiral blade in a tapering-off shape of which a tip part consecutively extends to the lower part of the hopper.SELECTED DRAWING: Figure 1
Patent JP-2021524702-A (JP)
Title: データ送信方法及び関連する装置
IPC: H04L47/20
Filed: 2019-04-04
Abstract: この出願の実施形態は、データ送信方法及び関連する装置を提供する。方法は、第１のノードによって、その分類属性値が第１の周波数ブロック分類範囲に属する送信されるべきフレームを、第１の周波数ブロックを利用することによって、第２のノードに送信するステップを含み、第１の周波数ブロックは、第１のノードと第２のノードとの間の少なくとも２つの周波数ブロックのうちの１つであり、送信されるべきフレームの分類属性値は、次の情報、即ち、フレームタイプ、送信レート、サービスの品質、サービス品質アクセスカテゴリ、空間ストリーム、送信持続期間、データパケットフォーマット、又はデータパケット帯域幅のうちの少なくとも１つを含む。この出願の実施形態において、第１のノードは、その分類属性値が第１の周波数ブロック分類範囲に属する送信されるべきフレームを、第１の周波数ブロックを利用することによって第２のノードに送信する。この場合において、周波数ブロックのスループットレートは増加されることができ、又は、周波数ブロックの全体のレイテンシレベルは低減されることができ、それによって、上位レイヤサービス要件の達成度が改善する。
Patent JP-2021519479-A (JP)
Title: 遺伝子検査とゲノム検査を識別するための人工知能と機械学習プラットフォーム
IPC: G16H50/30
Filed: 2019-03-19
Abstract: 遺伝子検査の識別性の改善は、健康関連変数の複数の異なる組み合わせを考慮した推奨遺伝子検査を含む第１の入力と、利用可能な遺伝子検査に関連する情報を含む第２の入力とを受け取る方法およびそれに付随するシステムを用いてなされる。これに基づいて、健康関連変数の異なる組み合わせと利用可能な遺伝子検査との間の複数のマッピングを含むルールのセットを生成する。ルールのセットを訓練データとして使用して分類器を訓練する。健康関連変数の第１の組み合わせ（健康関連変数の第１の組み合わせは、健康関連変数の複数の異なる組み合わせには含まれない）を含む第３の入力を受け取り、健康関連変数の第１の組み合わせを分類器への入力として提供し、分類器への入力に基づき、分類器からの出力として、利用可能な遺伝子検査から１種以上の推奨される遺伝子検査を受け取る。【選択図】 図３
Patent JP-2021529872-A (JP)
Title: 粘着剤組成物、これを含む粘着フィルム、粘着フィルムを含むバックプレートフィルムおよび粘着フィルムを含むプラスチック有機発光ディスプレイ
IPC: C09J133/06
Filed: 2019-07-29
Abstract: 本出願は、粘着剤組成物、これを含む粘着フィルム、粘着フィルムを含むバックプレートフィルムおよび粘着フィルムを含むプラスチック有機発光ディスプレイに関する。
Patent JP-2021530396-A (JP)
Title: 車両のタイヤ圧監視インターフェース
IPC: B60C23/04
Filed: 2018-07-19
Abstract: タイヤ圧監視システムインターフェースは、対応する複数の車両ホイールの複数のタイヤ圧センサの各々からタイヤ圧信号を受信するように構成される受信機と、警報装置と、タイヤ圧関連データのデータ選択を有する少なくとも１つのメニューを表示し、手動で入力された命令を受信するように構成される電子入出力装置と、電子メモリを有し、受信機、警報装置及び電子入出力装置に電子的に接続される電子コントローラとを備え、電子コントローラは、複数のホイールの各々について第１のタイヤ圧及び第２のタイヤ圧を定義するデータにアクセスするように電子メモリを動作させることであって、第１のタイヤ圧はオンロード走行条件用であり、第２のタイヤ圧はオフロード走行条件用であること、電子入出力装置を介して少なくとも１つのメニューを表示して、データ入力、データ選択及び動作命令のうちの少なくとも１つを受信することであって、命令はオフロード走行条件を要求することを含むこと、複数の車両ホイールの各々におけるタイヤ圧を監視すること、複数のホイールの各々におけるタイヤ圧の変化が、車両の外側でタイヤ圧を手動で変化させる人によって手動で達成されたかどうかをホイールごとに決定すること、及びタイヤ圧を手動で変化させる人に、各ホイールについてタイヤ圧の変化が達成されたことをホイールごとに警告するように警報装置を動作させることを行うように構成される。
Patent JP-2021531503-A (JP)
Title: 縁部撮像眼鏡レンズを有する拡張現実／仮想現実ニアアイ表示器
IPC: G02B27/02
Filed: 2019-07-16
Abstract: ニアアイ表示システムは、画像表示パネルと、プリズム組立体とを有し、プリズム組立体は、第１の要素及び第２の要素と、アイウェア眼鏡フレーム等の構造体とを備える。第１の要素及び第２の要素の前面及び後面は、プリズム組立体を形成するように位置合わせされ、接合される。部分反射コーティングは、第１の要素及び第２の要素の界面に施され、ビーム分割界面を画定する。画像表示パネルは、第１の要素の屈折面等の上側光学領域付近に配設される。ビーム分割界面の反対側の第２の要素の下側縁部は、コーティングされた反射面ミラーである。表示パネル、光学領域及び光反射面は、小型化をもたらし、対称性が崩れないように構成される。システムは、大きな瞳孔間距離（ＩＰＤ）のばらつき及び左目／右目を走らせる動作に適応する。【選択図】図１１
Patent JP-2021530849-A (JP)
Title: エネルギー貯蔵デバイス
IPC: H01M50/593
Filed: 2019-07-19
Abstract: エネルギー貯蔵デバイスを製造するための方法が提供される。スタックが基板上に設けられる。スタックは、第１の電極層、第２の電極層、および第１の電極層と第２の電極層との間の電解質層を含む。この方法は、第１の溝、第２の溝、および第３の溝を、基板のスタックの第２の面に対向するスタックの第１の面に形成することを含む。第１の溝は、第１の深さを有し、第１の表面は第２の電極層の第１の露出表面を含む。第２の溝は、第１の深さと異なる第２の深さを有し、第２の表面は第１の電極層の露出表面を含む。第３の溝は、第１の深さと実質的に同じ第３の深さを有し、第３の表面は第２の電極層の第２の露出表面を含む。
Patent JP-2022501326-A (JP)
Title: 自己免疫疾患の治療のための新規ピロリジンアミン化合物
IPC: A61K31/4709
Filed: 2018-09-07
Abstract: 本発明は、式（Ｉ）：（式中、Ｒ１、Ｒ２、Ｒ３、及びＲ４は、本明細書に記載するとおりである）の化合物、並びに、それらの薬学的に許容される塩、エナンチオマー、又はジアステレオマー、及び当該化合物を含む組成物、並びに、当該化合物の使用方法に関する。
Patent JP-2022500236-A (JP)
Title: 微多孔膜を処理する方法
IPC: B01D71/26
Filed: 2019-09-10
Abstract: 微多孔膜の表面を処理する方法は、（１）膜の少なくとも１つの表面を、（ａ）（ｉ）（メタ）アクリル酸モノマーおよび（ｉｉ）シラン官能性アクリルモノマーを含むビニルモノマーの混合物から調製したアクリルポリマー；ならびに（ｂ）塩基を含む処理用組成物と接触させるステップであって、アクリルポリマーが、マトリックス中に存在する充填剤と接触している、ステップと、（２）（１）の膜を、充填剤とアクリルポリマーとの間で縮合反応をもたらすに十分な条件に供するステップとを含む。処理された微多孔膜および水性処理用組成物も開示される。
Patent JP-2021524862-A (JP)
Title: 眼内圧を低下させるプロスタグランジンｆ２アルファ誘導体
IPC: A61P27/02
Filed: 2019-05-15
Abstract: 本発明は、臨床化学、特に、新規の生物学的に活性な化合物である、プロスタグランジンＦ２αのアミド誘導体に関する。これらの化合物は、低細胞毒性であり、哺乳類細胞中に内因性窒素酸化物の形成を刺激することができる。このような化合物の合成は、眼内圧を低下させることが可能な、プロスタグランジンＦ２αの生物学的に活性な誘導体の命名法の拡張を促進する。【選択図】 図１
Patent JP-2022501047-A (JP)
Title: 細胞内相図をマッピングするためのハイスループット法およびシステム
IPC: C12Q1/06
Filed: 2019-09-19
Abstract: 生体分子相互作用をマッピングまたはスクリーニングするためのハイスループット法およびシステムであって、方法が、複数の細胞、またはいくつかの場合、精製タンパク質自体を提供し、少なくとも１つの光波長によって制御可能なものを含む、相分離または凝集システムを発現する工程を含み、相分離または凝集システムが、場合によって蛍光タンパク質に融合しているターゲットタンパク質を含む、前記方法およびシステムを開示する。細胞をウェルに入れ、そして次いで、化学的および／または生物学的剤をウェルに導入する。次いで、ウェルを、相分離または凝集システムを制御する波長で照射し、その後、蛍光タンパク質またはフルオロフォアが蛍光を生じるようにウェルを照射し、その後、第一の領域が凝縮物を含有し、そして第二の領域が凝縮物を含有しない、試料の第一の領域および第二の領域内の蛍光の量に基づいて、相分離または凝集を定量化してもよい。光学的に制御されない相分離または凝集システムを利用する方法もまた開示する。【選択図】図１