Patent SG-11201407901P-A (SG)
Title: Single-particle analysis of particle populations
Inventor: ANDERSON MEGAN
IPC: C12Q1/68
Filed: 2013-05-21
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 28 November 2013 (28.11.2013) WIPOIPCT (10) International Publication Number WO 2013/177206 A2 (51) International Patent Classification: C12Q 1/68 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/042086 21 May 2013 (21.05.2013) English English (30) Priority Data: 61/649,845 21 May 2012 (21.05.2012) US (71) Applicant (for all designated States except US): FLUI- DIGM CORPORATION [US/US]; 7000 Shoreline Court, Suite 100, South San Francisco, California 94080 (US). (72) Inventors; and (71) Applicants (for US only)-. ANDERSON, Megan [US/US]; 2400 M Street NW, Apt. 439, Washington, Dis­ trict of Columbia 20037 (US). CHEN, Peilin [US/US]; 1023 Landmark Circle, Richmond, California 94806 (US). FOWLER, Brian [US/US]; 3362 Los Prados Street, San Mateo, California 94403 (US). KAPER, Fiona [NL/US]; 468 Santa Alicia, Solano Beach, California 92075 (US). LEBOFSKY, Ronald [CA/CA]; 79 Elsfield Road, Eto- bicoke, Ontario M8Y 3R7 (CA). MAY, Andrew [GB/US]; 564 29th Avenue, San Francisco, California 94121 (US). (74) Agents: HALIDAY, Emily M. et al.; Weaver Austin Vil- leneuve & Sampson LLP, P.O. Box 70250, Oakland, Cali­ fornia 94612-0250 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). [Continued on next page] (54) Title: SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS CS1 bd TS-F inimnraxEasjs3£LiiT\"iriiin.! : TS-R bcl CS2 CJ o CJ i> i> Off chip * A bc3 CS1 ^ A 3c4 CS1 A bc5 C$1 C52 hc3 B CS2 bc4 B C$2 hc5 B B C$2 hc5 Amplicons: All unique FIG. 10A i-H o CJ o & (57) Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analyt - ic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out \"in parallel,\" i.e., essentially simultaneously in the separate reaction volumes. WO 2013/177206 A2 I lllll llllllll II llllll III lllll lllll III III III lllll lllll llll lllll lllll lllll lllllll llll llll llll Published: — without international search report and to be republished upon receipt of that report (Rule 48.2(g))