Patent SG-11201810531T-A (SG)
Title: Ligand identification by co-fractionation
Inventor: SKIRYCZ ALEKSANDRA
IPC: G01N33/564
Filed: 2017-05-29
Abstract: AgurelMaterial 2.Lysis 3.Fractionation --10. 3452g 10' 128812g 45' 4b SEC 5. MTBE & LC/MS SECedutionvolum. 444 4 1 Fmdiong 44 4 W O 20 17 / 207 460 Al Input • . Lipids (upper phase) Polar (lower phase) Proteins (pellet) Time (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 07 December 2017 (07.12.2017) WIP0 I PCT omit VIII inn Holonmomiloolioluom (10) International Publication Number WO 2017/207460 Al (51) International Patent Classification: GO1N 33/564 (2006.01) GO1N 33/68 (2006.01) (21) International Application Number: PCT/EP2017/062841 (22) International Filing Date: 29 May 2017 (29.05.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 16172000.8 30 May 2016 (30.05.2016) EP (71) Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V. [DE/DE]; Hofgartenstr. 8, 80539 Miinchen (DE). (72) Inventors: SKIRYCZ, Aleksandra; Am Alten Mortel- werk 10, 14469 Potsdam (DE). KIERSZNIOWSKA, Sylwia; Glolmer Fichten 32, 14476 Potsdam (DE). VEYEL, Daniel; Gartenstr. 9, 14482 Potsdam (DE). WILLMITZER, Lothar; Waldwinkel 27, 14532 Klein- machnow (DE). CHODASIEWICZ, Monika; Storchen- hof 5, 14476 Potsdam (DE). (74) Agent: VOSSIUS & PARTNER (NO 31); SiebertstraBe 3, 81675 Miinchen (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 2 I (3)) (54) Title: LIGAND IDENTIFICATION BY CO-FRACTIONATION (57) : This invention relates to a method of determining ligands of macromolecules, said method comprising or consisting of (a) subjecting a sample comprising (i) complexes formed by said macromolecules and said ligands and (ii) unbound ligands to a method which separates said complexes from said un- bound ligands; (b) releasing ligands from complexes obtained in step (a); and (c) subjecting the released ligands obtained in step (b) to a chemical analysis method, thereby determining said ligands of said macromolecules. 4a Size Filtration 10. 3452g 30' 3452g 15' 10' 3452g 15' Input Wash Buffer Wash Buffer i Wash Buffer // ' C ' ,7- Flow Wash 100°c Elute