Patent GB-1106646-A (GB)
Title: Process for biosynthetically producing proteinaceous compositions
IPC: C12N1/26
Filed: 1965-10-29
Abstract: A high protein composition having a protein content in excess of 50% and an essential amino acid index in excess of 45 is produced by cultivating one (or more) of the following strains of micro-organisms:-  Pseudomonas ligustri (A.T.C.C. No. 15522) Pseudomonas pseudomallei (A.T.C.C. No. 15523)  Pseudomonas orvilla (A.T.C.C. No. 15524)  Alcaligenes sp. (A.T.C.C. No. 15525) Cellulomonas galba (A.T.C.C. No. 15526)  Brevibacterium insectiphilium (A.T.C.C. No. 15528)  Corynebacterium sp. (A.T.C.C. No. 15529)  Corynebacterium pourometabolum (A.T.C.C. No. 15530)  on an n-aliphatic hydrocarbon feed in a medium comprising an aqueous growth medium containing oxygen and other essential cell nutrients, employing temperatures ranging from 20 DEG  to 55 DEG  C., for a sufficient period of time to obtain the high protein composition, and harvesting the micro-organism cells.  At the end of the fermentation, the cells are isolated from the fermentation medium by decantation, filtration or centrifugation.  The filtered cells are then dewatered using a rotary drum dryer or spray dryer and are usually spray-dried at 150-185 DEG  C. for 2-30 seconds to render them nonviable. The fermentation broth, the microorganism cells and the proteinaceous compositions may be employed as an animal feed supplement, and in making glues and adhesives. Protein may be extracted by lysing with acetone, followed by basic or acidic extraction and isoelectric precipitation.ALSO:A high protein composition having a protein content in excess of 50 per cent. and an essential amino acid index in excess of 45 is produced by cultivating one (or more) of the following strains of micro-organisms:-  Pseudomonas ligustri (A.T.C.C. No.15522) Pseudomonas pseudomallei (A.T.C.C. No.15523) Pseudomonas orvilla (A.T.C.C. No.15524) Alcaligenes sp. (A.T.C.C. No.15525) Cellulomonas galba (A.T.C.C. No.15526) Brevibacterium insectiphilium (A.T.C.C. No.15528) Corynebacterium sp. (A.T.C.C. No.15529) Corynebacterium pourometabolum (A.T.C.C. No.15530) on an n-aliphatic hydrocarbon feed in a medium comprising an aqueous growth medium containing oxygen and other essential cell nutrients employing temperatures ranging from 20 DEG  to 55 DEG C., for a sufficient period of time to obtain the high protein composition, and harvesting the micro-organism cells.  The hydrocarbon feedstock preferably consists of C1 to C30 aliphatic hydrocarbons such as C6 to C10 light napthas, C11-C30 gas oils and petroleum fractions containing them. Normal C6-C30 mono- or polyolefines and branched aliphatic alkanes and olefins may be present in proportions of up to 30% by weight. Polycyclic aromatic compounds should be excluded.  The aqueous growth medium typically includes 4-120 g/l n-aliphatic hydrocarbon, 0.5-15 g/l potassium hydrogen phosphate, 5-15 g/l ammonium hydrogen phosphate, 0.1-10 g/l sodium sulphate, 0.1-0.7 g/l magnesium sulphate heptahydrate and 0.002-0.05 g/l of each of ferrous sulphate heptahydrate, manganese sulphate quartohydrate and sodium chloride. Many other ingredients are mentioned.  Oxygen may be provided by either an oxygen-enriched gas or oxygen-containing compounds.  The cultivation may be conducted batchwise in periods ranging from 30 minutes to 120 hours, or continuously; a pH in the range 6 to 8 is maintained.  At the end of the fermentation, the cells are isolated from the fermentation medium by decantation, filtration or centrifugation.  The filtered cells are then dewatered using a rotary drum dryer or spray dryer and are usually spray-dried at 150-185 DEG C. for 2-30 seconds to render them non-viable. The fermentation broth, the micro-organism cells and the proteinaceous compositions may be employed as an animal feed supplement, and in making glues and adhesives. Protein may be extracted by lysing with acetone, followed by basic or acidic extraction and iso-electric precipitation. The following essential amino acids are obtained from the harvested cells: arginine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, tyrosine and valine.  In the procedure of several Examples, n-hexadecane is employed as the hydrocarbon feedstock.  In one further Example, Alcaligenes sp. and Cellulomonas galba were cultivated first on a growth medium having 4-16% concentrations of n-hexadecane and then on a growth medium having 6-10% concentrations of n-amyl benzene.ALSO:A high protein composition having a protein content in excess of 50 per cent and an essential amino acid index in excess of 45 is produced by cultivating one (or more) of the following strains of micro-organisms:-  .Pseudomonas ligustri.(A.T.C.C. No. 15522) .Pseudomonas pseudomallei.(A.T.C.C. No. 15523) .Pseudomonas orvilla.(A.T.C.C. No. 15524) .Alcaligenes sp..(A.T.C.C. No. 15525) .Cellulomonas galba.(A.T.C.C. No. 15526) .Brevibacterium insectiphilium.(A.T.C.C. No. 15528) .Corynebacterium sp..(A.T.C.C. No. 15529) .Corynebacterium pourometalsolum.(A.T.C.C. No. 15530)  on an n-aliphatic hydrocarbon feed in a medium comprising an aqueous growth medium containing oxygen and other essential cell nutrients, employing temperatures ranging from 20 DEG  to 55 DEG C., for a sufficient period of time to obtain the high protein composition, and harvesting the micro-organism cells.  At the end of the fermentation, the cells are isolated from the fermentation medium by decantation, filtration or centrifugation.  The filtered cells are then dewatered using a rotary drum dryer or spray dryer and are usually spray-dried at 150-185 DEG C. for 2-30 seconds to render them non-viable.  The fermentation broth, the micro-organism cells and the proteinaceous compositions may be employed as an animal feed supplement, and in making glues and adhesives.  Protein may be extracted by lysing with acetone, followed by basic or acidic extraction and iso-electric precipitation.  The following essential amino acids are obtained from the harvested cells: arginine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, tyrosine and valine.