Patent SG-11201808976W-A (SG)
Title: Method of producing a recombinant protein
Inventor: EMMINS ROBYN ALEXANDRA
IPC: C12N15/70
Filed: 2017-05-04
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111011111111101 IIIIIIIIIIIIIIIIIII1011101111111101111111110111110111011111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\" WO 2017/191255 Al 09 November 2017 (09.11.2017) W I P0 I P C T — (51) (21) (22) (25) Filing Language: (26) (30) (71) (72) International Patent Classification: C07K 14/245 (2006.01) C12N International Application Number: International Filing Date: 04 Publication Language: Priority Data: 62/332,636 06 May 2016 (06.05.2016) 62/338,045 18 May 2016 (18.05.2016) 15/70 (2006.01) London Peter; PCT/EP2017/060654 Hertfordshire nage May 2017 (04.05.2017) Daniel; UDEN, English English Ioannis; sity US London US (74) Agent: INTELLECTUAL Patents LIMITED [GB/GB]; sex TW8 9GS (GB). Middlesex TW8 9GS (81) Designated kind GlaxoSmithK- AO, Hertfordshire SG1 CA, Gun- DZ, SG1 2NY (GB). HR, HOARE, Kenneth; Stevenage neering, Department of Biochemical Engineering, Univer- Michael; 32 Cherry Tree Road, East Finchley, Greater London N2 9QL (GB). LEWIS, Alan GlaxoSmithKline, Gunnels Wood Road, Stevenage SG1 2NY (GB). LEWIS, William John GlaxoSmithKline, Gunnels Wood Road, Steve- Hertfordshire SG1 2NY (GB). MCINALLY, Adam 1/2 9 Yorkhill Parade, Glasgow G3 8SW (GB). Mark; GlaxoSmithKline, Gunnels Wood Road, Hertfordshire SG1 2NY (GB). VOULGARIS, c/o The Advanced Centre for Biochemical Engi- College London, Bernard Katz Building, Gordon Street, WC1H OAH (GB). HARRISON, Anna; GlaxoSmithKline, Global Applicant: GLAXOSMITHKLINE PROPERTY DEVELOPMENT 980 Great West Road, Brentford (GB). Inventors: EMMINS, Robyn Alexandra; line, Gunnels Wood Road, Stevenage 2NY (GB). FINKA, Gary Brian; GlaxoSmithKline, nels Wood Road, Stevenage Hertfordshire CN925.1, 980 Great West Road, Brentford Middle- States (unless otherwise indicated, for every of national protection available): AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, = Title: METHOD OF PRODUCING A 0.2 : The present invention relates comprising the signal sequence, a sequence can be used to provide a method of the recombinant protein. (54) protein N expression A RECOMBINANT PROTEIN 044 0.11 0.15 Figure 5 D.07 0.04 9.16 0.13 = = = — _ — = — = = = = = = = .V. . .1.:1 . to a recombinant signal sequence recombinant protein and methods of producing the recombinant protein. The recombinant for controlling the viscosity of the fermentation, and/or controlling basal (pre-induction) -,, . derived from E. Coli. The invention also relates to a fusion Il in in ei 1-1 cT 1-1 ----. (57) IN 0 signal C [Continued on next page] WO 2017/191255 Al MIDEDIMOMOIDEIROIDEMOMOHHOH1MODEVOIMIE KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a))