Patent NZ-629703-A (NZ)
Title: Methods and compositions for detecting multiple analytes with a single signal
Inventor: SICILIANO NICHOLAS
IPC: G01N33/543
Filed: 2013-03-07
Abstract: A method of concurrently detecting a first analyte of interest and a second analyte of interest in a test sample comprises contacting a solid support (10) with the test sample comprising a first analyte of interest (20) and a second analyte of interest (40), a bridge unit (30) comprising a second capture reagent, and a signal detection unit (60) comprising a third capture reagent. The first analyte of interest from the sample and the second analyte of interest from the sample are different; and detecting the presence or absence of the signal detection unit which indicates the presence or absence of the first analyte of interest and second analyte of interest in the sample concurrently with a single signal. The single signal is detected only when the first analyte of interest from the sample, the second analyte of interest from the sample, and the bridge unit form a bridging complex and the signal detection unit binds to the bridging complex. The first capture reagent (15) is affixed to the solid support. The first analyte of interest from the sample comprises a first interaction unit that binds to the first capture reagent and a second interaction unit that binds to the bridge unit; and the second analyte of interest from the sample comprises a first interaction unit and a second interaction unit. The first interaction unit of the second analyte of interest binds the bridge unit. The first and second interaction unit of the first analyte of interest from the sample and the first and second interaction unit of the second analyte of interest from the sample are each, independently, a heterologous interaction unit. The signal detection unit that binds to: i) the second analyte from the sample; ii) to the second analyte’s first interaction unit or second interaction unit; iii) to a component of the bridging complex; or iv)a component of the bridging complex that is only present when the complex contains the first and second analytes from the sample. A complex comprises a solid support, a first analyte of interest and a second analyte of interest from a test sample, a bridge unit, and a signal detection unit. The first analyte of interest comprises a first interaction unit and a second interaction unit and the second analyte of interest comprises a first interaction unit and a second interaction unit. The first and second interaction unit of the first analyte of interest from the sample and the first and second interaction unit of the second analyte of interest from the sample are each, independently, a heterologous interaction unit when the first analyte of interest from the sample, the second analyte of interest from the sample, and the bridge unit form a bridging complex and the signal detection unit binds to the bridging complex. The signal detection unit binds to: i) the second analyte, ii) to the second analyte’s first interaction unit or second interaction unit, iii) to a component of the first and second analyte complex, or iv)a component of an analyte-bridge complex that is only present when the complex contains the first and second analytes. A complex comprises a solid support (10), a first analyte of interest (20), a second analyte of interest (40), a bridge unit (30), and a signal detection unit (60). Each member of the complex binds to each other directly or indirectly.