Patent DE-102010026369-A1 (DE)
Title: Dynamic breeding, reproduction and/or differentiation of stem cells or primary cells of human and animal origin, comprises adhering stem cells or primary cells of human and animal origin on cell carriers, culturing, dynamically cultivating
Inventor: HOFFMEISTER HANS PROF DR
IPC: C12M1/42
Filed: 2010-07-07
Abstract: Dynamic breeding, reproduction and/or differentiation of stem cells or primary cells of human and animal origin, comprises: adhering the stem cells or primary cells of human and animal origin on cell carriers or implant surface, culturing by dynamic supply of nutrient medium, carrying out the cultivation, propagation or differentiation in an overlay-atmosphere; dynamically cultivating the adherent cells on cell carriers or implant surface; and simultaneously subjecting to constant and/or fluctuating pressure load. Dynamic breeding, reproduction and/or differentiation of stem cells or primary cells of human and animal origin, comprises: adhering the stem cells or primary cells of human and animal origin on cell carriers or implant surface, culturing by dynamic supply of nutrient medium, carrying out the cultivation, propagation or differentiation in an overlay-atmosphere, where a rotating bed (6) driven by a magnetic actuator is designed as the cell carriers or implant surface in a reactor housing (1); dynamically cultivating the adherent cells on cell carriers or implant surface; and simultaneously subjecting to constant and/or fluctuating pressure load. The cell carrier or implant surface is rotationally moved through the culture medium in the reactor housing and through the overlay atmosphere in the head-space of the reactor housing alternately at a constant rotation speed of the rotating bed of 0.1-20 revolutions per minute, preferably 0.5-2 revolutions per minute. An independent claim is also included for a pressure-rotating bed bioreactor for dynamic breeding, reproduction and/or differentiation of stem cells or primary cells of human and animal origin, comprising a pressure-resistant reactor housing made of borosilicate glass with pressure-resistant lids, which are detachably arranged on the sides, tightly fastened to the reactor housing and are made of stainless steel, where a storehouse is provided in the two lids (2, 3) for accommodating a shaft or a receiving device (4). The rotating bed comprising the cell carriers or implant surface is attached to the shaft or receiving device. The rotating bed is used as a package of cell carriers or implant surface arranged over one another, with defined distance from each other parallel to the shaft or receiving device of the rotating bed through the rectangular slabs, which are supported by lateral receivers different respectively to the diameter of the reactor housing adjusted width or as a package of cell carrier or implant surface with round discs having defined spaces. The rotating bed is arranged with adjusted diameter of the reactor housing, which is arranged perpendicular to the shaft or receiving device on the shaft or receiving device or other conventional cell carriers or implant surface, which are used as rotating beds. The rotating beds are driven by a permanent magnet (7) arranged on the shaft or receiving device, or rotated by magnetic actuator outside the reactor housing in rotation. The lids are provided with ports for the supply and discharge of culture- and nutrient medium. The ports for the supply and discharge of overlay atmosphere are provided in the inlets and outlets for the culture- and nutrient medium. The pressure control valves are arranged in proportional technology for inlet and outlet of overlay atmosphere.