Patent CA-2597526-A1 (CA)
Title: New bridge between two known techniques + mass cloning that works. never been tried before.
Inventor: VOON GERARD
IPC: C12Q1/68
Filed: 2007-09-11
Abstract: 1. We could micro inject a generic version of the below mix of chemicals into stem cells and electric stimulate the cell to divide and also add growth factors such as Wnt, telomerase and epigenetic drugs (and mix of temperature changes), to cause the stem cells to and DNA within to proliferate. 2. Synthesizing (Cloning) mass (from stem cells) DNAs (for Humans and Animals) - see below - and then micro inject or electric fuse into a (reminded by AR) mass assembly line of nucleus removed Oocytes from multiple donors. Then electric pulse/shock them to fuse the DNA into the donor's cytoplasm in the Oocytes and electric shock (or heat shock) to divide At the prime Blastocyst to remove the stem cells and use to grow muscle tissue, neurons, blood, bone marrow .. We could open Oocyte banks. 3. We are going to try to skip the recombinant (or try both with or without plasmid recombinant) stage with the bacterial plasmid (below), but rather remove the DNA  from a eukaryoitic bacterial cells, microinject or electric fuse a human/animal/reptile/amphibian/fish DNA to the cytoplasm of the bacteria and then electric shock to stimulate the bacteria cell to divide and possibly use growth factors and epigenetic drugs to enhance proliferation. Once we need to grow specialized stem cells, we take an individual bacterial cell with the human DNA and remove the DNA then micro inject (electric fuse) the human DNA into a Oocyte (whose nucleus -DNA has been removed) (fuse the DNA to the Oocyte's cytoplasm) of a donor human female we could collect Oocyte in mass quantity. Electric shock (or heat shock) the cell into division. Then collect the human stem cells from the blastocyst, and tissue culture, use growth factors, eg. telomerase, Wnt, and use epigenetic drugs to continue to multiply, then use the stem cells to grow muscle tissue, neurons, blood, bone marrow... 4 We are also looking at bioreactors filled with either cytoplasm (or mix of a solution containing the materials below - eliminating the need for cytoplasm) taken from mass bacteria cells and organelles surrounding the large number of farmed DNA that is always constantly replicating and kept alive for a long time, possibly with tube to feed nutrients, oxygen and waste removal, studying pressure needs and membranes maybe holders with valleys the length width of DNAs or mix them all together;  sealed with a membrane above (taking into consideration (optimal chemical needs - see solution and primers below, surrounding overcrowding, density, protein (receptors) -enzymes (and their relationship to cell to cell signalling), chemical, energy molecules, pH maintenance, primers, MG2+, MgCl2, HCL, EDTA, KCL...), ammonium sulfate, ATP, GTP, NTP, EDTA and Proteinase K" and/or Centrifugation. While in the this mass farm of DNA, we could electric shock (or heat shock, alternating heat and cold -the solution is heated to separate the DNA strands. When it is cooled, the polymerase creates a copy of each strand. The process is repeated every five minutes until the desired amount of DNA is produced. - for exact details eg. exact temperature, see below) to stimulate the DNA to replicate and synthesize including addition of Polymerase Chain Reaction and and Ligase (the ase... family of enzymes).  Ethanol can used to purify the template DNA very carefully (highly purify) so not to inhibit the PCR process. To add to the DNA denaturation we can add glycerol, DMSO, or formamide