Patent AU-2019301062-A1 (AU)
Title: Micro flow-through electroporation devices and methods of cell transfection
Inventor: CHANG CHIH-WEI
IPC: C12M1/42
Filed: 2019-07-09
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) 
(19) World Intellectual Property 
(1) Organization11111111111111111111111I1111111111111i1111liiiii 
International Bureau (10) International Publication Number 
(43) International Publication Date W O 2020/014264 Al 
16 January 2020 (16.01.2020) W IPO I PCT 
(51) International Patent Classification: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, 
C12M 3/06 (2006.01) C12M1/42 (2006.01) HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 
(21) International Application Number: KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 
PCT/US2019/041055 MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, 
OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 
(22) International Filing Date: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 
09 July 2019 (09.07.2019) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.  
(25) Filing Language: English (84) Designated States (unless otherwise indicated, for every 
kind of regional protection available): ARIPO (BW, GH, 
(26)PublicationLanguage: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, 
(30) Priority Data: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 
62/695,436 09 July 2018 (09.07.2018) US TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 
EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, 
(71)Applicant: NANOCAV, LLC [US/US];9920Jefferson MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, 
Boulevard,CulverCity,CA90232(US). TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, 
(72) Inventor: CHANG, Chih-Wei; c/o NantBio, Inc., 9920 KM, ML, MR, NE, SN, TD, TG).  
Jefferson Boulevard, Culver City, CA 90232 (US).  
Published: 
(74) Agent: KORAL, Elisabeth et al.; Harness, Dickey & - with international search report (Art. 21(3)) 
Pierce, P.L.C., 7700 Bonhomme Ave., Suite 400, Clayton, - with amended claims (Art. 19(1)) 
MO 63105 (US).  
(81) Designated States (unless otherwise indicated, for every 
kind of national protection available): AE, AG, AL, AM, 
AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 
CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, 
(54) Title: MICRO FLOW-THROUGH ELECTROPORATION DEVICES AND METHODS OF CELL TRANSFECTION 
FIG. 2B 
offset-mesh 
Surface: Electric field norm (kV/cm 
0.3 - 16 
0.2 1,4 
0.1
12 
0 > 
0. ............................. 3 
__0.3 
-0.4 0 -0.5 0, 
-0.6 0.2 
-0.7 flow 
0.4 0.6 0.8 1 1,2 1.4 1.6 1.8 
(57) Abstract: Systems and methods are provided for transfecting cells, such as mammalian cells and nonmammalian cells, using an 
electroporation apparatus having an electroporation chamber including an upper micromesh electrode, a lower micromesh electrode 
CA and a path defined in the electroporation chamber. The electroporation apparatus includes a first input allowing passage of cells into the 
electroporation chamber and a first output allowing passage of electroporated cells from the electroporation chamber. The first input 
and the first output are separated by an offset distance.