Patent SG-11201909364U-A (SG)
Title: Controlling translocating molecules through a nanopore
Inventor: TABARD-COSSA VINCENT
IPC: C12Q1/68
Filed: 2018-04-27
Abstract: ccO O O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 November 2018 (01.11.2018) WIPO I PCT 01E101°1a °nolo Holollm iflo oimIE (10) International Publication Number WO 2018/201038 Al (51) International Patent Classification: C12Q 1/68 (2018.01) (21) International Application Number: PCT/US2018/029939 (22) International Filing Date: 27 April 2018 (27.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/491,572 28 April 2017 (28.04.2017) US (71) Applicants: THE UNIVERSITY OF OTTAWA [CA/CA]; 3042-800 King Edward, Ottawa, Ontario K1N 6N5 (CA). UNIVERSITY OF ROCHESTER MEDICAL CENTRE [US/US]; 601 Elmwood Avenue, Rochester, New York 14642 (US). (72) Inventors: TABARD-COSSA, Vincent; 40 Saint- Francois, Gatineau, Quebec J9A 1A9 (CA). BRIGGS, Kyle; 210 Stewart Street, Apt.27, Ottawa, Ontario K1N 6K2 (CA). MADEJSKI, Gregory; 3231 Eagle Harbor Wa- terport Rd, Albion, New York 14411 (US). MCGRATH, James; 2 Shrewsbury Lane, Fairport, New York 14450 (US). (74) Agent: MACINTYRE, Timothy D. et al.; Harness, Dickey & Pierce, P.L.C., P.O. Box 828, Bloomfield Hills, Michigan 48303 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) (54) Title: CONTROLLING TRANSLOCATING MOLECULES THROUGH A NANOPORE (57) : To reduce unwanted variation in the speed of DNA translocating solid-state nanopores, a nanoscale pre-confinement of translocating molecules is demonstrated using an ultra-thin nanoporous silicon nitride (NPN) membrane separated from a single sensing nanopore by a nanoscale cavity. Comprehensive experimental results demonstrate that the presence of this nanofilter results in a global minimum in the coefficient of variation of passage times in the sensing pore over a range of DNA sizes which depends on the height of the cavity. Such advanced nanopore devices minimize the standard deviation of the passage time distribution independently of its diameter and stability. These results also represents the first experimental verification that the inter-and intra-molecular passage time variation depends on the conformational entropy of such molecule prior to translocation, while providing a practical strategy for controlling transport across solid-state nanopores.