Patent EA-002242-B1 (EA)
Title: Mammalian-derived peptides for the treatment of microbial infection
Inventor: HOFFMAN BRIAN F
IPC: A01N63/50
Filed: 1998-08-10
Abstract: 1. A method for killing bacteria or fungi comprising contacting the bacteria or fungi with an antimicrobially effective amount of mammalian hemoglobin protein, hemoglobin protein fragment or polypeptide fragments thereof selected from the group consisting of α-chain hemoglobin, heme-free hemoglobin β-chain, heme-free hemoglobin chain, a hemoglobin α-3 fragment, a hemoglobin β-2 fragment, an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, an amino acid sequence of SEQ ID NO: 5, an amino acid sequence of SEQ ID NO: 6, fragments of said proteins or polypeptide fragments thereof and combinations thereof. 2. The method according to Claim 1, wherein said hemoglobin protein, hemoglobin protein fragment or polypeptide fragments are derived from humans. 3. The method according to Claim 1, wherein the bacteria are Gram-negative bacteria. 4. The method according to Claim 3, wherein the Gram-negative bacteria are selected from the group consisting of Escherichia coli and Pseudomonas aeruginosa. 5. The method according to Claim 1 wherein the bacteria are Gram-positive bacteria. 6. The method according to Claim 5 wherein the Gram-positive bacteria are selected from the group consisting of Streptococcus aureus and Streptococcus faecalis. 7. The method according to Claim 1, wherein the fungi are Candida albicans. 8. A method for treating a subject having a bacterial or fungal infection which comprises administering to the subject an antimicrobially effective amount of mammalian hemoglobin protein, hemoglobin protein fragment or polypeptide fragments thereof selected from the group consisting of α-chain hemoglobin, heme-free, hemoglobin β-chain, heme-free hemoglobin chain, a hemoglobin α-3 fragment, a hemoglobin β-2 fragment, an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, an amino acid sequence of SEQ ID NO: 5, an amino acid sequence of SEQ ID NO: 6, fragments of said proteins or polypeptide fragments thereof and combinations thereof. 9. The method according to Claim 8, wherein said hemoglobin protein, hemoglobin protein fragment or polypeptide fragments are derived from humans. 10. The method according to Claim 8, wherein the bacteria are Gram-negative bacteria. 11. The method according to Claim 10, wherein the Gram-negative bacteria are selected from the group consisting of Escherichia coli and Pseudomonas aeruginosa. 12. The method according to Claim 8 wherein the bacteria are Gram-positive bacteria. 13. The method according to Claim 12 wherein the Gram-positive bacteria are selected from the group consisting of Streptococcus aureus and Streptococcus faecalis. 14. The method according to Claim 8, wherein the fungi are Candida albicans. 15. A method for treating material subject to bacterial or fungal contamination, comprising applying or admixing with said material an antimicrobially effective amount of mammalian hemoglobin protein, hemoglobin protein fragment or polypeptide fragments thereof selected from the group consisting of α-chain hemoglobin, heme-free, hemoglobin β-chain, heme-free hemoglobin chain, a hemoglobin α-3 fragment, a hemoglobin β-2 fragment, an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, an amino acid sequence of SEQ ID NO: 5, an amino acid sequence of SEQ ID NO: 6, fragments of said proteins or polypeptide fragments thereof and combinations thereof. 16. The method according to Claim 15, wherein said hemoglobin protein, hemoglobin protein fragment or polypeptide fragments are derived from humans. 17. The method according to Claim 15, wherein the bacteria are Gram-negative bacteria. 18. The method according to Claim 17, wherein the Gram-negative bacteria are selected from the group consisting of Escherichia coli and Pseudomonas aeruginosa. 19. The method according to Claim 15 wherein the bacteria are Gram-positive bacteria. 20. The method according to Claim 19 wherein the Gram-positive bacteria are selected from the group consisting of Streptococcus aureus and Streptococcus faecalis. 21. The method according to Claim 15, wherein the fungi are Candida albicans. 22. A pharmaceutical dosage form comprising an antimicrobially effective amount of mammalian hemoglobin protein, hemoglobin protein fragment or polypeptide fragments thereof selected from the group consisting of α-chain hemoglobin, heme-free, hemoglobin β-chain, heme-free hemoglobin chain, a hemoglobin α-3 fragment, a hemoglobin β-2 fragment, an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, an amino acid sequence of SEQ ID NO: 5, an amino acid sequence of SEQ ID NO: 6, fragments of said proteins or polypeptide fragments thereof and combinations thereof. 23. The pharmaceutical dosage form according to Claim 22, wherein said hemoglobin protein, hemoglobin protein fragment or polypeptide fragments are derived from humans. 24. A polypeptide selected from the group consisting of an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, , an amino acid sequence of SEQ ID NO: 5, , an amino acid sequence of SEQ ID NO: 6, , an amino acid sequence of SEQ ID NO: 8, , an amino acid sequence of SEQ ID NO: 9 and fragments of said sequences. 25. The use of a mammalian hemoglobin protein, hemoglobin protein fragment or polypeptide fragments thereof selected from the group consisting of α-chain hemoglobin, heme-free, hemoglobin β-chain, heme-free hemoglobin chain, a hemoglobin α-3 fragment, a hemoglobin β-2 fragment, an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, an amino acid sequence of SEQ ID NO: 5, an amino acid sequence of SEQ ID NO: 6, fragments of said proteins or polypeptide fragments thereof and combinations thereof for antimicrobial treatment of bacteria or fungi. 1. A method for killing bacteria or fungi comprising contacting the bacteria or fungi with an antimicrobially effective amount of mammalian hemoglobin protein, hemoglobin protein fragment or polypeptide fragments thereof selected from the group consisting of α-chain hemoglobin, heme-free hemoglobin β-chain, heme-free hemoglobin chain, a hemoglobin α-3 fragment, a hemoglobin β-2 fragment, an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, an amino acid sequence of SEQ ID NO: 5, an amino acid sequence of SEQ ID NO: 6, fragments of said proteins or polypeptide fragments thereof and combinations thereof. 2. The method according to Claim 1, wherein said hemoglobin protein, hemoglobin protein fragment or polypeptide fragments are derived from humans. 3. The method according to Claim 1, wherein the bacteria are Gram-negative bacteria. 4. The method according to Claim 3, wherein the Gram-negative bacteria are selected from the group consisting of Escherichia coli and Pseudomonas aeruginosa. 5. The method according to Claim 1 wherein the bacteria are Gram-positive bacteria. 6. The method according to Claim 5 wherein the Gram-positive bacteria are selected from the group consisting of Streptococcus aureus and Streptococcus faecalis. 7. The method according to Claim 1, wherein the fungi are Candida albicans. 8. A method for treating a subject having a bacterial or fungal infection which comprises administering to the subject an antimicrobially effective amount of mammalian hemoglobin protein, hemoglobin protein fragment or polypeptide fragments thereof selected from the group consisting of α-chain hemoglobin, heme-free, hemoglobin β-chain, heme-free hemoglobin chain, a hemoglobin α-3 fragment, a hemoglobin β-2 fragment, an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, an amino acid sequence of SEQ ID NO: 5, an amino acid sequence of SEQ ID NO: 6, fragments of said proteins or polypeptide fragments thereof and combinations thereof. 9. The method according to Claim 8, wherein said hemoglobin protein, hemoglobin protein fragment or polypeptide fragments are derived from humans. 10. The method according to Claim 8, wherein the bacteria are Gram-negative bacteria. 11. The method according to Claim 10, wherein the Gram-negative bacteria are selected from the group consisting of Escherichia coli and Pseudomonas aeruginosa. 12. The method according to Claim 8 wherein the bacteria are Gram-positive bacteria. 13. The method according to Claim 12 wherein the Gram-positive bacteria are selected from the group consisting of Streptococcus aureus and Streptococcus faecalis. 14. The method according to Claim 8, wherein the fungi are Candida albicans. 15. A method for treating material subject to bacterial or fungal contamination, comprising applying or admixing with said material an antimicrobially effective amount of mammalian hemoglobin protein, hemoglobin protein fragment or polypeptide fragments thereof selected from the group consisting of α-chain hemoglobin, heme-free, hemoglobin β-chain, heme-free hemoglobin chain, a hemoglobin α-3 fragment, a hemoglobin β-2 fragment, an amino acid sequence of SEQ ID NO: 3, an amino acid sequence of SEQ ID NO: 2, an amino acid sequence of SEQ ID NO: 5, an amino acid sequence of SEQ ID NO: 6, fragments of said proteins or polypeptide fragments thereof and combinations thereof. 16. The method according to Claim 15, wherein said hemoglobin protein, hemoglobin protein fragment or polypeptide fragments are derived from humans. 17. The method according to Claim 15, wherein the bacteria are Gram-negative bacteria. 18. The method according to Claim 17, wherein the Gram-negative bacteria are selected from the group consisting of Escherichia coli and Pseudomonas aeruginosa. 19. The method according to Claim 15 wherein the bacteria are Gram-positive bacteria. 20. The method according to Claim 19 wherein the Gram-positive bacteria are selected from the group consisting of Streptococcus