Patent SG-11201903429Q-A (SG)
Title: Molecular rheostat for cofactor balance
Inventor: BOWIE JAMES
IPC: C12P7/16
Filed: 2017-10-18
Abstract: TopGlycolysis Hex Pgi Pfk Ald Tpi () INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 April 2018 (26.04.2018) WIP0 I PCT 0111111010 0111 °nolo oviniliolommovoimIE (10) International Publication Number WO 2018/075624 Al (51) International Patent Classification: C12P 19/36 (2006.01) C12P 7/16 (2006.01) (21) International Application Number: PCT/US2017/057156 (22) International Filing Date: 18 October 2017 (18.10.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/409,731 18 October 2016 (18.10.2016) US (71) Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA [US/US]; 1111 Franklin Street, 12th Floor, Oakland, CA 94607-5200 (US). (72) Inventors: BOWIE, James, U.; 4243 Le Bourget Av- enue, Culver City, CA 90232 (US). KORMAN, Tyler, P.; 290 W. Laurel Avenue, Sierra Madre, CA 91024 (US). OPGENORTH, Paul, H.; 1839 South Beverly Glen Boule- vard, Apt #5, Los Angeles, CA 90025 (US). (74) Agent: BAKER, Joseph, R., Jr.; Gavrilovich, Dodd & Lindsey LLP, 4370 La Jolla Village Drive, Suite 303, San Diego, CA 92122 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). = (54) Title: MOLECULAR RHEOSTAT FOR COFACTOR BALANCE (57) : The disclosure provides a metabolic pathway for producing a metabolite, the metabolic pathway having a co-factor regulatory system for cofactor utilization in the metabolic pathway. 2x Gald-3-P Molecular Rheosta t Non-Stoichiornetric Stoithiometric x Pi .-,.: mGap f tivity: Hydrolysis jr x 1,3 BPG x ADP---,.: Pgk x ATP* , : >, • 2x NADP + GapN -2x 2x NADPH W O 20 18/075 6 24 Al AlsS nye Ih,D KivD YahlS 2x 3 PG Bottom Glycolysis 4 Isobutanol FIG. 1 Pgm Eno Pyk 2x NADP 2x NADP 4 \" [Continued on next page] WO 2018/075624 Al MIDEDIMOM0101011EFIEHMOMMEHOIDIOHEMOVOIS Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))