Patent SG-11201808302V-A (SG)
Title: Methods and systems for determining a drop delay of a flow stream in a flow cytometer
Inventor: NORTON PIERCE
IPC: G01N15/14
Filed: 2017-06-14
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#111111101110101011111 3E1 0 011101E11111 111111111111111111 0111111 Organization 0 International Bureau (10) International Publication Number (43) International Publication Date .....0 WO 2018/067210 Al 12 April 2018 (12.04.2018) W I PO I PC T (51) International Patent Classification: (74) Agent: FIELD, Bret, E.; 201 Redwood Shores Parkway, GO1N 15/14 (2006.01) Suite 200, Redwood City, California 94065 (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2017/037508 kind of national protection available): AE, AG, AL, AM, (22) International Filing Date: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 14 June 2017 (14.06.2017) CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (25) Filing Language: English HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (26) Publication Language: English KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (30) Priority Data: OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 62/403,553 03 October 2016 (03.10.2016) US SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicant: BECTON, DICKINSON AND COMPANY [US/US]; 1 Becton Drive, MC110, Franklin Lakes, New (84) Designated States (unless otherwise indicated, for every Jersey 07417-1880 (US). kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (72) Inventor: NORTON, Pierce, 0.; 16280 Greenridge Ter- UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, race, Los Gatos, California 95032 (US). TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, Title: METHODS AND SYSTEMS FOR DETERMINING A DROP DELAY OF A FLOW STREAM IN A FLOW CYTOME- (54) = TER = = Step 1 = • = = Step 2 = = = = Step 3A Step 3B = = = _ = Step 4A Step 4B = 1 ,-1 d © ,-1 Step 5 FIG. 1 ei IN (57) : Methods and systems for determining a drop delay of a flow stream in a flow cytometer are provided. Aspects of the ' t:: methods according to certain embodiments include capturing an image of the flow stream to obtain an imaged flow stream, identifying 0 ---- a disturbance at a break off point in the imaged flow stream and calculating the drop delay of the flow stream based on the identified GO ,_ 1 disturbance. Systems for practicing the subject methods having an imaging sensor for capturing one or more images of the flow stream 0 and a processor configured to identify a disturbance at a break off in the imaged flow stream and calculating the drop delay using the N imaged flow stream are also provided. Non-transitory computer readable storage mediums are also described. C [Continued on next page] WO 2018/067210 Al MIDEDIMOMMIDIREEMOTINHOHOMMOVOIS MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))