Patent SG-11201809482T-A (SG)
Title: Systems and methods for enriching target cells in a sample
Inventor: BHAGAT ALI ASGAR S
IPC: G01N15/10
Filed: 2016-05-04
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property C.--.` Organization 3 MD HIM 0 11101 HOE 010 1111 1 0 I 011111010101101111111101110 HOED International Bureau (10) International Publication Number (43) International Publication Date .......\"' WO 2017/192098 Al 09 November 2017 (09.11.2017) WIP0 I PCT (51) International Patent Classification: (72) Inventors: BHAGAT, Ali Asgar S.; 63 Hume Avenue, C12M 1/00 (2006.01) B81B 1/00 (2006.01) #10-05, Singapore 598742 (SG). WU, Andrew En-Tzu; GO1N 15/10 (2006.01) Fl 6K 99/00 (2006.01) 37 Lorong 5 Toa Payoh, #15-355, Singapore 310037 (SG). BOIL 3/00 (2006.01) HUANG, Junquan; Blk 139B #19-60 Lorong lA Toa Payoh, Singapore 312139 (SG). GUAN, Guofeng; 1 Nor- (21) International Application Number: PCT/SG2016/050205 manton Park, #13-153, Singapore 118998 (SG). CHAO, Shuzhe; Clementi Ave 4, BLK 305 #05-425, Singapore (22) International Filing Date: 120305 (SG). KOH, Yau Luong; 21 Jansen Road, Singa- 04 May 2016 (04.05.2016) pore 548412 (SG). LEE, Yifang; 29 Daisy Avenue, Singa- (25) Filing Language: English pore 359505 (SG). LEONG, Man Chun; APT BLK 297A, #12-30, Compassvale street, Singapore 541297 (SG). (26) Publication Language: English (74) Agent: PATEL, Upasana; Marks & Clerk Singapore LLP, (71) Applicant: CLEARBRIDGE BIOMEDICS PTE LTD Tanjong Pagar, P 0 Box 636, Singapore 910816 (SG). [SG/SG]; 81 Science Park Drive, #02-03 Singapore Science (81) Designated States (unless otherwise indicated, for every Park 1, Singapore 118257 (SG). kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, _ (54) Title: SYSTEMS AND METHODS 300 -\"\-> Pump 328 326 324 322 FOR ENRICHING TARGET CELLS IN A SAMPLE 310 318 362 chip comprising a upstream end of the channel, the directed to the waste an output flow rate a sample collection microfluidic chip chip is coupled to = = = Cartridge with Inertial Microfluidic Enrichment Chip — = 312 Stemple D' il ' Eml . Sample 16 314 3 Collect • Valve Purnpa ... es. 0 1 X.* * i• • : : 344 . . ,,: „. 4 '\",: Oilusn 11 I 0 1 . 7 t) (1 : . E. h al 352 Valve 2 • '; le a or all m //it. : *4 imateullei6 • . • Waste = = NI — — 334 342 Output • Controller 1 :--- Output 2 354 = _ 370 360 3 a microfluidic to an of the microfluidic cells are chip; chip; output of the microfluidic = = — = = = — I nputs I Outputs 1 320 350 330 332 340 Figure : A system for enriching target cells in a sample is disclosed. The system comprises inlet, a sample outlet, a waste outlet and a microfluidic channel, the sample inlet being coupled channel and the sample outlet and the waste outlet being coupled to a downstream end chip being configured such that target cells are directed to the sample outlet and non-target a sample input pump configured to introduce the sample into the sample inlet of the microfluidic configured to measure a flow rate at the sample outlet and / or the waste outlet of the microfluidic valve having a configuration switchable between a first configuration in which the sample coupled to a sample output container and a second configuration in which the sample output of the (57) c:::: , microfluidic N microfluidic ---, detector N switching = _ = 1-1 GO sample 01 ON outlet; Il Il cz is N C [Continued on next page] WO 2017/192098 Al MIDEDIMOMOIDEIRMEMOMOHEIDIONIMODEVON EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) a waste output container; and a controller configured to control flow rate measured by the output flow rate detector. the configuration of the sample collection switching valve using the