Patent SG-11201806453T-A (SG)
Title: Improved blood-brain barrier endothelial cells derived from pluripotent stem cells for blood-brain barrier models
Inventor: PARK TAE-EUN
IPC: G01N33/50
Filed: 2017-02-16
Abstract: FIG3 4 a 6 7 8 8000 ts 'E 6000 U E 4000 ce al 200: I- W O 20 17 / 1430 49 Al Normoxia dypoxia Estimated in vivo RBB TEO? value $13$ chip Niue (-601: Days of culture on TEER chip (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/143049 Al 24 August 2017 (24.08.2017) WIPO I PCT 1111111111111101110111111111110101111101110101111101111111111111110111111111110111111 (51) International Patent Classification: C12N 5/095 (2010.01) G01N 27/403 (2006.01) GO1N 27/40 (2006.01) G01N 33/50 (2006.01) (21) International Application Number: PCT/US2017/018150 (22) International Filing Date: 16 February 2017 (16.02.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/295,693 16 February 2016 (16.02.2016) US (71) Applicant: PRESIDENT AND FELLOWS OF HAR- VARD COLLEGE [US/US]; 17 Quincy Street, Cam- bridge, Massachusetts 02138 (US). (72) Inventors: PARK, Tae-Eun; 400 Brookline Avenue, Bo- ston, Massachusetts 02115 (US). HERLAND, Anna; 19 Vernon Street, Brookline, Massachusetts 02246 (US). FITZGERALD, Edward Anthony; 28 Ellison Avenue, Boston, Massachusetts 02126 (US). INGBER, Donald El- liot; 162 West Canton Street, Boston, Massachusetts 02118 (US). (74) Agents: RESNICK, David S. et al.; Nixon Peabody LLP, 100 Summer St., Boston, Massachusetts 02110-2131 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. ( 84 ) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.1700) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) [Continued on next page] (54) Title: IMPROVED BLOOD-BRAIN BARRIER ENDOTHELIAL CELLS DERIVED FROM PLURIPOTENT STEM CELLS FOR BLOOD-BRAIN BARRIER MODELS (57) : The invention relates to a method of creating a human blood-brain barri- er (BBB) model from the differentiation of human pluripotent stem cells (hPSCs), wherein the BBB exhibits sustained transen- dothelial electrical resistances (TEER) over 2000 Q. cm' for at least 3 days after seeding. WO 2017/143049 Al 1#1101MOMOIDEIROIDEOMOIDEMIDOINEMOMOVOIMIE Published: — with international search report (Art. 21(3))