Patent SG-11201901518W-A (SG)
Title: Methods for producing virus for vaccine production
Inventor: RAO RAMAN
IPC: C12N7/02
Filed: 2017-09-01
Abstract: 6 days riusicm culture W O 20 18/0 45 3 44 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 March 2018 (08.03.2018) WIPO I PCT Iiiimmommionotioloolommoillmommom oimiE (10) International Publication Number WO 2018/045344 Al (51) International Patent Classification: A61K 39/125 (2006.01) CO7K 14/105 (2006.01) A61K 39/13 (2006.01) Cl 2N 7/02 (2006.01) C07K 14/085 (2006.01) (21) International Application Number: PCT/US2017/049956 (22) International Filing Date: 01 September 2017 (01.09.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/382,621 01 September 2016 (01.09.2016) US (71) Applicants: TAKEDA VACCINES, INC. [US/US]; One Takeda Parkway, Deerfield, IL 60015 (US). TAKEDA PHARMACEUTICAL COMPANY LIMITED [JP/JP]; 1-1, Doshomachi 4-Chome, Chuo-ku, Osaka-shi, Osaka, 541-0045 (JP). (72) Inventors: RAO, Raman; c/o Takeda Vaccines Pte. Ltd., 21 Biopolis Rd., Nucleos North Tower Level 4, Singa- pore 138567 (SG). SATO, Tatsuki; c/o Takeda Pharma- ceutical Company Limited, 4720, Aza-Takeda, Oaza-Mit- sui, Hikari-shi, Yamaguchi, 743-0011 (JP). ODA, Kaori; c/o Takeda Pharmaceutical Company Limited, 4720, Aza- Takeda, Oaza-Mitsui, Hikari-shi, Yamaguchi, 743-0011 (JP). NAKAMURA, Kuniaki; c/o Takeda Pharmaceu- tical Company Limited, 4720, Aza-Takeda, Oaza-Mit- sui, Hikari-shi, Yamaguchi, 743-0011 (JP). NISHIHAMA, Takeshi; c/o Takeda Pharmaceutical Company Limited, 4720, Aza-Takeda, Oaza-Mitsui, Hikari-shi, Yamaguchi, 743-0011 (JP). (74) Agent: LITTLEFIELD, Otis et al.; Morrison & Foerster LLP, 425 Market Street, San Francisco, CA 94105-2482 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (54) Title: METHODS FOR PRODUCING VIRUS FOR VACCINE PRODUCTION FIG. 1 To Downstream Process (57) : The present disclosure relates to methods of producing Enterovirus C, e.g., for poliomyelitis vaccine production. In some embodiments, the methods include adding polysorbate to the cell culture medium during or prior to inoculation with the virus and/or culturing cells in a fixed bed bioreactor. Further provided herein is an Enterovirus C produced by the methods of production disclosed herein, as well as compositions, immunogenic compositions, and vaccines related thereto. [Continued on next page] WO 2018/045344 Al MIDEDIMOMMIDIRERIONOIMMIMOIMMOIMIE HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))