Patent SG-11201909797Q-A (SG)
Title: Methods for generating genetically modified animals
Inventor: YANG LUHAN
IPC: C12N5/075
Filed: 2018-04-20
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 25 October 2018 (25.10.2018) WIPO I PCT onion °nolo mu 100 Ho INN oimIE (10) International Publication Number WO 2018/195402 Al W O 20 18/ 195 40 2 Al (51) International Patent Classification: AO1K 67/027 (2006.01) Cl 2N 15/873 (2006.01) C12N 5/075 (2010.01) Cl 2N 15/877 (2006.01) C12N 5/16 (2006.01) Cl 2N 15/90 (2006.01) (21) International Application Number: PCT/US2018/028539 (22) International Filing Date: 20 April 2018 (20.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/487,898 20 April 2017 (20.04.2017) US 62/500,197 02 May 2017 (02.05.2017) US 62/527,702 30 June 2017 (30.06.2017) US 62/543,610 10 August 2017 (10.08.2017) US (71) Applicant: EGENESIS, INC. [US/US]; 300 Technology Square, Suite 301, Cambridge, MA 02139 (US). (72) Inventor: YANG, Luhan; c/o Egenesis, Inc., 300 Technol- ogy Square, Suite 301, Cambridge, MA 02139 (US). (74) Agent: FOURNIER, David, B. et al.; Perkins Coie LLP, P.O. Box 1247, Seattle, WA 98111-1247 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) = (54) Title: METHODS FOR GENERATING GENETICALLY MODIFIED ANIMALS (57) : The present disclosure provides methods of generating multiplexed genetically modified animals, for example, porcine endogenous retrovirus (PERV)- inactivaied pigs. The disclosure also provides methods of improving the birth rate of multiplexed genetically modified animals. In some embodiments, the present dis- closure is concerned with the generation and utilization of porcine cells in which porcine endogenous retroviral (PERV) elements have been inactivated. In sonic em- bodiments, the PERV-free or PERV-reduced porcine cells are cloned to produce porcine embryos. In some embodiments, the PERV-free or PERV-reduced embryos may be grown into adult swine from which organs and/or tissues may be extracted and used for such purposes as xenotransplantation into non-porcine animals such as humans. FIGURE 1613