Patent SG-11201808022W-A (SG)
Title: Selection and cloning of t lymphocytes in a microfluidic device
Inventor: BRONEVETSKY YELENA
IPC: C12N5/0783
Filed: 2017-03-16
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11111111011110111010101111101011111011101111011011101110101101111101111011111 International Bureau ... ......jd (10) International Publication Number ......„.,./ (43) International Publication Date WO 2017/161210 Al 21 September 2017 (21.09.2017) WIP0 I PCT (51) International Patent Classification: (74) Agents: BREIER, Adam M. et al.; McNeill Baur PLLC, C12N 5/0783 (2010.01) C12M 1/24 (2006.01) 11501 Dublin Boulevard, Suite 200, Dublin, California G01N 35/08 (2006.01) BOlD 37/00 (2006.01) 94568 (US). G01N 33/00 (2006.01) (81) Designated States (unless otherwise indicated, for every (21) International Application Number: kind of national protection available): AE, AG, AL, AM, PCT/US2017/022846 AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (22) International Filing Date: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, 16 March 2017 (16.03.2017) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (25) Filing Language: English KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (26) Publication Language: English NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (30) Priority Data: RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, 62/309,454 17 March 2016 (17.03.2016) US TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, 62/326,667 22 April 2016 (22.04.2016) US ZA, ZM, ZW. 62/412,212 24 October 2016 (24.10.2016) US (84) Designated States (unless otherwise indicated, for every 62/470,744 13 March 2017 (13.03.2017) US kind of regional protection available): ARIPO (BW, GH, (72) Inventors; and GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, _ (71) Applicants : BRONEVETSKY, Yelena [US/US]; 5858 TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Horton Street, Suite 320, Emeryville, California 94608 TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (US). WANG, Xiaohua [CN/US]; 5858 Horton Street, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Suite 320, Emeryville, California 94608 (US). BEEM-LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, - — ILLER, Peter J. [US/US]; 5858 Horton Street Suite 320, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Emeryville, California 94608 (US). BEAUMONT, GW, KM, ML, MR, NE, SN, TD, TG). Kristin G. [US/US]; 5858 Horton Street, Suite 320, Published: = Emeryville, California 94608 (US). LOWE, JR., Randall — D. [US/US]; 5858 Horton Street, Suite 320, Emeryville, with international search report (Art. 21(3)) = California 94608 (US). MASTROIANNI, Alexander J. — before the expiration of the time limit for amending the = [US/US]; 5858 Horton Street, Suite 320, Emeryville, Cali- claims and to be republished in the event of receipt of fornia 94608 (US). CHAPMAN, Kevin T. [US/US]; 5858 amendments (Rule 48.2(h)) = Horton Street, Suite 320, Emeryville, California 94608 = (US). = = = = = = = = 1-1 © 11 \" (54) Title: SELECTION AND CLONING OF T LYMPHOCYTES IN A MICROFLUIDIC DEVICE 1-1 1-1 (57) : Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing ---- one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and per - fusing IN culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to un- dergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced ac - N cording to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating can- t on. ) cer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a ''t microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lympho - P.- cytes into the subject.