Patent SG-11201907277X-A (SG)
Title: A high throughput 3d assay for immune cell and drug homing, migration and tumor cytotoxicity
Inventor: SHERMAN HILARY A
IPC: C12Q1/02
Filed: 2018-02-06
Abstract: coco O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 16 August 2018 (16.08.2018) WIP0 1 PCT iii1111111 01E1111111111111111111111111111111111111111111111111111111111110111111111111 (10) International Publication Number WO 2018/148208 Al (51) International Patent Classification: C12N 5/09 (2010.01) C12Q 1/02 (2006.01) (21) International Application Number: PCT/US2018/017081 (22) International Filing Date: 06 February 2018 (06.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/455,881 07 February 2017 (07.02.2017) US (71) Applicant: CORNING INCORPORATED [US/US]; 1 Riverfront Plaza, Corning, New York 14831 (US). (72) Inventor; and (71) Applicant: SHERMAN, Hilary A. [US/US]; 59 Freshet Road, Madbury, New Hampshire 03823 (US). (74) Agent: WILKS, Susan S; Corning Incorporated, Intellec- tual Property Department, SP-Ti-03, Corning, New York 14831 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (54) Title: A HIGH THROUGHPUT 3D ASSAY FOR IMMUNE CELL AND DRUG HOMING, MIGRATION AND TUMOR CYTOTOXICITY 400 400 FIG. 5A FIG. 5B FIG. 5C (57) : The present disclosure relates to methods for performing assays for active migration and cytotoxicity of a therapeutic agent towards tumor cells, e.g., immune cell and/or drug homing, migration, and tumor cytotoxicity. The methods are performed in labware that provide opportunities for a therapeutic agent, such as an immune cell or a drug, to migrate toward tumor cells, including tumor cells growing in a 3D spheroid conformation. The methods allow for, among other uses, the investigation of the effects of a therapeutic agent, such as immune cells or a drug, on tumor cells, and enable the investigation of homing, tumor cytotoxicity, and tumor immune evasion in a single, easy-to-use, high throughput system for more in vivo-like testing.