Patent SG-11201805166P-A (SG)
Title: Light-mediated polymerase chain reaction amplification and product detection system and methods of use
Inventor: BIRO RONALD L
IPC: B01L7/00
Filed: 2017-03-08
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111010111110111011111011011011101111101111101111011111 International Bureau .. .... ..Yejd (10) International Publication Number (43) International Publication Date .... ...,,, WO 2017/156126 Al 14 September 2017 (14.09.2017) WIP0 I PCT (51) International Patent Classification: BOIL 3/00 (2006.01) G01N 21/64 (2006.01) BOIL 7/00 (2006.01) G01N 35/02 (2006.01) KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (21) International Application Number: PCT/US2017/021359 TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (22) International Filing Date: (84) Designated States (unless otherwise indicated, for every 8 March 2017 (08.03.2017) kind of regional protection available): ARIPO (BW, GH, (25) Filing Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (26) Publication Language: English TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (30) Priority Data: 62/306,432 10 March 2016 (10.03.2016) US DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, (71) Applicant: PIONEER HI-BRED INTERNATIONAL, INC. [US/US]; 7100 NW 62ND AVE, PO BOX 1014, Johnston, Iowa 50131-1014 (US). GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: (72) Inventors: BIRO, Ronald L; 7250 NW 62nd Avenue, PO as to applicant's entitlement to apply for and be granted a Box 552, Johnston, Iowa 50131-0552 (US). HUNT, Beau; patent (Rule 4.17(u)) 1010 NE 5th Street, Ankeny, Iowa 50021-2065 (US). — as to the applicant's entitlement to claim the priority of the _ Agent: STIMSON, Michael J.; 7250 NW 62ND AVE, (74) earlier application (Rule 4.17(iii)) PO BOX 552, Johnston, Iowa 50131-0552 (US). Published: Designated States (unless otherwise indicated, for every (81) — with international search report (Art. 21(3)) = kind of national protection available): AE, AG, AL, AM, _ before the expiration of the time limit for amending the AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) — = _ = = = = = = = = = 1-1 ,tD ei ,-1 ,tD kin ,-1 (54) Title: LIGHT-MEDIATED POLYMERASE CHAIN REACTION AMPLIFICATION AND PRODUCT DETECTION SYS - 11 TEM AND METHODS OF USE 0 N (57) : A PCR amplification and product detection system is disclosed. The system utilizes a uniform and direct photonic en. ) heating subsystem to mediate reaction-by-reaction, high-throughput PCR amplification detectable by a fluorescence detection sub - system. Reaction-by-reaction temperature monitoring for dynamic feedback heat regulation is also disclosed. Also disclosed are methods for using the same.