Patent SG-11201805973W-A (SG)
Title: Rodents having an engineered heavy chain diversity region
Inventor: MACDONALD LYNN
IPC: C07K16/00
Filed: 2017-01-12
Abstract: DNterm Nterm rev trans W O 20 17 / 12380 4 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/123804 Al 20 July 2017 (20.07.2017) WIPO I PCT (51) International Patent Classification: A01K 67/027 (2006.01) C07K 16/00 (2006.01) (21) International Application Number: PCT/US2017/013247 (22) International Filing Date: 12 January 2017 (12.01.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/278,127 13 January 2016 (13.01.2016) US (71) Applicant: REGENERON PHARMACEUTICALS, INC. [US/US]; 777 Old Saw Mill River Road, Tarrytown, New York 10591-6707 (US). (72) Inventors: MACDONALD, Lynn; c/o Regeneron Phar- maceuticals, Inc., 777 Old Saw Mill River Road, Tarry- town, New York 10591 (US). MCWHIRTER, John; c/o Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591 (US). MURPHY, An- drew J.; c/o Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Road, Tarrytown, New York 10591 (US). (74) Agents: WU, Rita S. et al.; 410 Seventeenth Street, Suite 2200, Denver, Colorado 80202 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: with international search report (Art. 21(3)) [Continued on next page] (54) Title: RODENTS HAVING AN ENGINEERED HEAVY CHAIN DIVERSITY REGION 28 AID hotspots (10 natural+18 artificial) D6 Mem Figure IA (57) : Non-human animals and methods and compositions for making and using them are provided, wherein said non-hu- man animals have a genome comprising an engineered or recombinant diversity cluster within an immunoglobulin heavy chain vari- able region, which engineered or recombinant diversity cluster comprises an insertion of one or more coding sequences of a non-im- munoglobulin polypeptide of interest. Non-human animals described herein express antibodies characterized by complementary de- termining regions (CDRs), in particular, CDR3s having diversity that directs binding to particular antigens. Methods for producing antibodies from non-human animals are also provided, which antibodies contain human variable regions and mouse constant re- gions. 111111111111110111011111111111010111110 01111111101111110111111111111111111110111111 WO 2017/123804 Al 1#1101MO1101DEIROIDEOM0110111101101110EMOVON before the expiration of the time limit for amending the — with sequence listing part of description (Rule 5.2(a)) claims and to be republished in the event of receipt of amendments (Rule 48.2(h))