Patent GB-475868-A (GB)
Title: Process for the production of dry preparations from entire glands, and more particularly from the animal pancreas
IPC: A61K35/39
Filed: 1936-04-22
Abstract: Dry enzymatic preparations are obtained from entire animal glands, particularly the pancreas, by subjecting the pulped gland to extraction at ordinary temperature with anhydrous acetone or anhydrous azeotropric mixtures containing acetone in a closed system, subjecting the extract to reflux distillation to free the solvent from water and extracted substances, recycling the solvent until the pulp is dehydrated and defatted and freeing it in situ from the residual solvent.  The liquid residue remaining after distilling off the solvent is precipitated, e.g. by addition of neutral salts, whereby substances of the nature of protective colloids are recovered which may be added in liquid or solid form to the dry gland or its aqueous preparations.  An apparatus suitable for carrying out the process comprises an extractor 1 provided with a false bottom 3, a stirrer 2 and heating coil 4, solvent receptacles 11, 12 and a storage tank 13 from which solvent may be forced up into the receptacles.  The extractor may be connected alternatively with a vessel 19 or with a condenser 23 communicating with absorption tanks 25-27, all of which communicate with the solvent still 21.  In the process of extraction solvent from the vessels 11, 12 is allowed to flow on to the comminuted gland placed in the extractor and after standing is passed on through the vessel 19 into the still 21, the vapours from which are <PICT:0475868/IV/1> condensed by the packing 36 and cooling coil 37 sufficiently to remove water, the solvent passing on and being condensed by coil 40.  At the conclusion of the extraction the extractor is put into communication with the condenser 23, steam is supplied to the coil 4 and a vacuum is applied at 28 the solvent being thus removed from the pulp remaining in the extractor and recovered subsequently from the absorption tanks 25-27 which may contain bisulphite of soda.  The extract remaining in the still 21 is drawn off at 33.ALSO:Dry enzymatic preparations are obtained from entire animal glands, particularly the pancreas, by subjecting the pulped gland to extraction at ordinary temperature with anhydrous acetone or anhydrous azeotropic <PICT:0475868/III/1> mixtures containing acetone in a closed system, subjecting the extract to reflux distillation to free the solvent from water and extracted substances, recycling the solvent until the pulp is dehydrated and defatted and freeing it in situ from the residual solvent.  The liquid residue remaining after distilling off the solvent is precipitated, e.g. by addition of neutral salts, whereby substances of the nature of protective colloids are recovered which may be added in liquid or solid form to the dry gland or its aqueous preparations. An apparatus suitable for carrying out the process comprises an extractor 1 provided with a false bottom 3, a stirrer 2 and heating coil 4, solvent receptacles 11, 12 and a storage tank 13 from which solvent may be forced up into the receptacles.  The extractor may be connected alternatively with a vessel 19 or with a condenser 23 communicating with absorption tanks 25--27, all of which communicate with the solvent still 21.  In the process of extraction solvent from the vessels 11, 12 is allowed to flow on to the comminuted gland placed in the extractor and after standing is passed on through the vessel 19 into the still 21, the vapours from which are condensed by the packing 36 and cooling coil 37 sufficiently to remove water, the solvent passing on and being condensed by coil 40.  At the conclusion of the extraction the extractor is put into communication with the condenser 23, steam is supplied to the coil 4 and a vacuum is applied at 28 the solvent being thus removed from the pulp remaining in the extractor and recovered subsequently from the absorption tanks 25--27 which may contain bisulphite of soda.  The extract remaining in the still 21 is drawn off at 33.