Patent SG-11201901000X-A (SG)
Title: Compositions and methods of cell attachment
Inventor: LEVNER DANIEL
IPC: G01N33/50
Filed: 2017-07-12
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 18 January 2018 (18.01.2018) W I PO I PCT ill~~~~~~~~ 011101010VIIIOH olo omm offilm °nom° oimIE (10) International Publication Number WO 2018/013718 Al (51) International Patent Classification: C12M 1/12 (2006.01) G01N 33/50 (2006.01) C12N 5/07 (2010.01) (21) International Application Number: PCT/US2017/041762 (22) International Filing Date: 12 July 2017 (12.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/361,259 12 July 2016 (12.07.2016) US (71) Applicant: EMULATE, INC. [US/US]; 27 Drydock, Boston, MA 02210 (US). (72) Inventors: LEVNER, Daniel; 55 Sewall Avenue, 5C, Brookline, MA 02446 (US). HUH, Dongeun, Huh; 3411 Chestnut Street, #433, Philadelphia, PA 19104 (US). JANG, Kyung, Jin; 2 Greenmeadow Lane, Andover, MA 01810 (US). FRASER, Jacob; 19 Kingston Street, Apt. 2, Somerville, MA 02144 (US). KERNS, Jordan; 7 Her- itage Drive, Reading, MA 01867 (US). VARONE, Anto- nio; 4975 Washinton Street, Unit 118, West Roxbury, MA 02132 (US). (74) Agent: CARROLL, Peter, G. et al.; Medlen & Carroll, LLP, 1440 Broadway, Suite 1010, Oakland, CA 94612 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) = (54) Title: COMPOSITIONS AND METHODS OF CELL ATTACHMENT Sulfa-SAN PAH Suifosaccinintidyl e-14 . -aido-2'-riitrophonvlaminathexaaava PAW 492.40 1-1 Spacer Am 18,2 A cc FIGURE 1 N O (57) : Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in cc a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as O mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed. C