Patent SG-11201808957U-A (SG)
Title: Penicillin-g acylases
Inventor: NAZOR JOVANA
IPC: C12P21/00
Filed: 2017-05-05
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111 IOU 011101011M 010 1111 1 OM 0111111110101101 OH OE 11110 1111 0E111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"1 WO 2017/193022 Al 09 November 2017 (09.11.2017) WIP0 I PCT — (51) (21) (22) (25) Filing Language: (26) (30) (71) (72) Inventors: NAZOR, Jovana; 200 Penobscot Drive, Red- International Patent Classification: car; C12N 9/84 (2006.01) C12P 21/00 (2006.01) (US). wood International Application Number: toria PCT/US2017/031342 ifornia International Filing Date: Penobscot 05 May 2017 (05.05.2017) (74) Agent: English 200 Publication Language: English (US). (81) Designated Priority Data: kind 62/332,103 05 May 2016 (05.05.2016) US AO, Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- CA, ye, Redwood City, California 94063 (US). DZ, HR, KW, wood City, California 94063 (US). MITCHELL, Ves- MK, na; 200 Penobscot Drive, Redwood City, California 94063 PA, (US). ELGART, David; 200 Penboscot Drive, Redwood SD, City, California 94063 (US). LEXA, Katrina, W.; 126 TT, East Lincoln Ave, Rahway, New Jersey 07065 (US). DEL- LAS, Nikki; 200 Penobscot Drive, Redwood City, Cali- (84) Designated fornia 94063 (US). ORR, Robert, Kevin; 126 East Lin- kind coln Ave, Rahway, New Jersey 07065 (US). ALVIZO, Os- GM, 200 Penobscot Drive, Redwood City, California 94063 GARCIA, Ravi, David; 200 Penobscot Drive, Red- City, California 94063 (US). VIDUYA, Judy, Vie- Antonio; 200 Penobscot Drive, Redwood City, Cal- 94063 (US). MOFFETT, Courtney, Dianne; 200 Drive, Redwood City, California 94063 (US). MACKNIGHT, Kamrin, T.; CODEXIS, INC., Penobscot Drive, Redwood City, California 94063 States (unless otherwise indicated, for every of national protection available): AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. States (unless otherwise indicated, for every of regional protection available): ARIPO (BW, GH, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, - = = Title: PENICILLIN 1000 800 600 400 200 : The encoding such enzymes, compositions including the enzymes, and methods of using the enzymes. (54) el cleotides -G ACYLASES Insulin 1\ b Al Al 61 al B1 829 Al B29 B29 k Al Bi 629 = = = = = _ = = = = = = — = 1-1 ei ei © M c:r ,—, IN ,—, 0 (57) 0.25 0.5 0.75 1 1.25 1.5 1.25 2 2.25 FIG. 1 present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynu- [Continued on next page] WO 2017/193022 Al IMEDIMOM0101011MMEMOMOMMIONEEHINEVOIMIE UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) — with sequence listing part of description (Rule 5.2(a))