Patent DE-102009011755-A1 (DE)
Title: Electroporation reactor for pressured electroporation of biological commercial- and waste material as process material in continuous process throughput, comprises two roller groups with a roller and a fluid permeable transport device
Inventor: BERGHOEFER THOMAS
IPC: C12M1/42
Filed: 2009-03-04
Abstract: The electroporation reactor for pressured electroporation of biological commercial- and waste material as process material (4) in continuous process throughput, comprises two roller groups (1, 2) with a roller (3) forming a gap (6) at adjustable distance to each other, a fluid permeable transport device (7) that rotates during the process over guiding rollers positioned external of the vessel. The rotational axis of all rollers lies horizontal and parallel to each other and all rollers of both roller groups rotate in process with respective equal peripheral speed. The electroporation reactor for pressured electroporation of biological commercial- and waste material as process material (4) in continuous process throughput, comprises two roller groups (1, 2) with a roller (3) forming a gap (6) at adjustable distance to each other, a fluid permeable transport device (7) that rotates during the process over guiding rollers positioned external of the vessel. The rotational axis of all rollers lies horizontal and parallel to each other and all rollers of both roller groups rotate in process with respective equal peripheral speed in transport direction of the process material. Fluid-filled vessel is completely submerged in the gap formed though both roller groups. The transport device draws during the process with the peripheral speed of the rollers through the gap formed by roller groups. The roller group is connected to an electric reference potential and the other roller group is connected with a central subgroup directly adjacent rollers, directly or over the fluid indirectly over a field discharge electrode at the exit of the voltage source or both roller groups are divided in a similar subgroup of adjacent rollers. The central subgroups are connected electrically-directed or over the fluid indirectly over a fixed field discharge electrode on the adjacent exit of an earth symmetrical voltage source. The connection line of the roller middle point passes one of the roller groups to the connection line of the roller middle point of another roller group or converges in transport direction of the process material. The successive subgroups of the roller group are electric-conductively connected over the fluid. The rollers are made of mechanically deformable material inert against the process material. The roller group lying within the curvature of the transport device consists of a roller of electric conductive material, whose diameter is larger than the group opposite to the roller. The rollers of both roller groups are equipped on its both ends with an electric field discharge profile. The ends of the roller of both roller groups exist in shadow of the field discharge electrode. The transport device consisting of transport band arranged on a layer made of electrically conductive rods connected over insulation tape, where the rods have an interval of 1-20 mm. The voltage source is a direct current voltage source, alternating current voltage source or high voltage impulse generator. An independent claim is included for a method for pressured electroporation of biological commercial- and waste material as process material in continuous process throughput.