Patent SG-11201807223U-A (SG)
Title: CANCER TREATMENT BASED ON DELIVERY OF OLIGOES VIA GAP JUNCTIONS FROM HUMAN MESENCHYMAL STEM CELLS (hMSC)
Inventor: COHEN IRA S
IPC: C12P19/34
Filed: 2017-03-23
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111141101110101011111 HO 11111011101111011110110011111011111110111011# International Bureau .. .... ..Yejd (10) International Publication Number (43) .... ...,,, International Publication Date WO 2017/165641 Al 28 September 2017 (28.09.2017) WIP0 I PCT (51) International Patent Classification: (74) Agents: MOLINELLI, Eugene J. et al.; P.O. Box 231, A01N 43/04 (2006.01) CO7H 21/00 (2006.01) Manassas, Virginia 20108 (US). A61N 1/362 (2006.01) CO7H 21/04 (2006.01) A61P 35/00 (2006.01) C12P 19/34 (2006.01) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (21) International Application Number: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, PCT/US2017/023803 BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FL GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, 23 March 2017 (23.03.2017) KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (25) Filing Language: English MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (26) Publication Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (30) Priority Data: TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, 62/312,230 23 March 2016 (23.03.2016) US ZA, ZM, ZW. (71) Applicants: THE TRUSTEES OF COLUMBIA UNI- (84) Designated States (unless otherwise indicated, for every VERSITY IN THE CITY OF NEW YORK [US/US]; kind of regional protection available): ARIPO (BW, GH, 412 Low Memorial Library, 535 West 116th Street, New GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, York, New York 10027 (US). THE RESEARCH TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, _ FOUNDATION for THE STATE UNIVERSITY OF TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, NEW YORK [US/US]; 35 State Street, Albany, New York DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, 12207 (US). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, — _ SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, (72) Inventors: COHEN, Ira S.; 23 Hawks Nest Road, Stony GW, KM, ML, MR, NE, SN, TD, TG). Brook, New York 11790 (US). BRINK, Peter R.; 101 Dyke Road, New York 11733 ROSEN, Declarations under Rule 4.17: = Setauket, (US). Michael R.; 25 East 86th Street, Apt 14C, New York, New — as to applicant's entitlement to apply for and be granted a York 10028 (US). patent (Rule 4.1700) = = = [Continued on next page] = (54) Title: CANCER TREATMENT BASED ON DELIVERY OF OLIGOES VIA GAP JUNCTIONS FROM HUMAN MESEN - STEM CELLS (hMSC) CHYMAL = _ _ 1.6 = = — M 1.2 u — Control = = = c ' 0.8 c m — Z Treated — > 0.4 -1 8 1-1 E .4 M 0.0 ~r~ , 1-1 iv 0 10 20 30 40 Days kr) V; ,-1 FIG.19A IN (57) 1-1 : A method of treating cancer in vivo includes introducing in vitro into human mesenchymal stem cells (hMSCs) at C least one type of inhibitory oligonucleotide, and contacting a tumor tissue of syncytial cancer cells with the hMSCs in vivo under N conditions permitting a hMSC to form a gap junction channel with a first syncytial cancer cell of the tumor tissue. As a result, the at en. ) least one type of inhibitory oligonucleotide is delivered into the first syncytial cancer cell by traversing the gap junction channel and 7 1 ,1 9 the at least one type of inhibitory oligonucleotide is delivered into a second syncytial cancer cell of the tumor tissue by traversing a gap junction channel between the first syncytial cancer cell and the second syncytial cancer cell. WO 2017/165641 Al MIDEDIM01111E111 IIIIIOINIE0111111111111111111100111101111111011110111111 — as to the applicant's entitlement to claim the earlier application (Rule 4.17(iii)) Published: the priority of — with sequence listing part of description (Rule 5.2(a)) — with international search report (Art. 21(3))