Patent CA-2696047-C (CA)
Title: A method for identifying, expanding, and removing adult stem cells and cancer stem cells
Inventor: CLEVERS JOHANNES CAROLUS
IPC: C12N5/095
Filed: 2008-08-08
Granted: 2023-10-03
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY  (PCT) (19) World IntellectuM Property Organization 1111111111 111111 0111 11111111111 1 11 iilNllNlHlllllllllflInumm International Bureau (43) International Publication Date    PCT    (10) International Publication Number 19 February 2009 (19.02.2009)                  WO 2009/022907 A3 (51) International Patent Classification:                                   (74) Agent: HATZMANN, M.J.; Vereenigde, Johan de Witt-C12N 5/06 (2006.01)             CO7K 14/72 (2006.01)    laan 7, NL-2517 JR Den Haag (NL). C12N 5/08 (2006.01)             A 6IK 38/00 (2006.01) (81) Designated States (unless otherwise indicated, for every (21) International Application Number:                                      kind of national protection available): AE, AG, AL, AM, PCT/NI2008/050543                    AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, (22) International Filing Date: 8 August 2008 (08.08.2008)                 EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, (25) Filing Language:                 English                              LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, (26) Publication Language:            English                              RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TT, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 07114192.3                            10 August 2007 (10.08.2007) EP (84) Designated States (unless otherwise indicated for every kind of regional protection available): ARIPO (BW, GH, (71) Applicants (for all designated States except US):                     GM, KE, LS, MW, IWZ, NA, SD, SL, SZ, TZ, UG, ZM, HUBRECHT INSTITUUT [NUNL]; Uppsalalaan 8,    ZW), Eurasian (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM), =                                     NL-3584 CT Utrecht (NL). KONINKLIJKE  NEDER-European (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, F1, LANDSE AKADEMIE VAN WETENSCHAPPEN FR, GB, GR, HR, HU, lE, IS, IT, LT, LU, IN, MC, MT, NL, [M./NU; Kloveniersburgwal 29, NL-1011 JV Amsterdam    NO, PL, PT, RO, SE, SI, SK, TR), OAPI (BF, BJ, CF, CG, (NL). CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). (72) Inventors; and    Published: T.= (75) Inventors/Applicants (for US only): CLEVERS, Jo- ¨ with international search report =                                                                           hannes Carolus INLINL1; Ruysdaellaan 7, NL-3712 AP ¨ before the expiration of the dine limit for amending the Huis ler Heide (NL). BARKER, Nicholas [GB/NL];    claims and to be republished in the event of receipt of Mijdrechtstraat 66, NL-3522 HX Utrecht (NL). HAEGE-    amendments BARTH, Andrea PE/DE]; Antonstrasse 33, 13347 Berlin (DE). VAN DE WETERING, Marcus Lambertus (88) Date of publication of the international search report: [NUNL]; Kievitwiede 15, NL-3993 DA Houten (NL).                            25 June 2009 (54) Title: A METHOD FOR IDENTIFYING, EXPANDING, AND REMOVING ADULT STEM CELLS  AND CANCER STEM  CELLS ¨    (57) Abstract: The invention relates to the fieldsof biochemistry, pharmacyand oncology. The invention particularly relates to the -    use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in -    for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. -= In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6or 5 binding compound, identify the -    cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further ¨    relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells. cIN