Patent SG-11201402502R-A (SG)
Title: Populations of hematopoietic progenitors and methods of enriching stem cells therefor
Inventor: KELLER GORDON
IPC: C12Q1/24
Filed: 2012-11-21
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 30 May 2013 (30.05.2013) WIPOIPCT (10) International Publication Number WO 2013/075222 A1 (51) International Patent Classification: C12N 5/0789 (2010.01) C12Q 1/24 (2006.01) C12N 5/071 (2010.01) (21) International Application Number: PCT/CA2012/001076 (22) International Filing Date: (25) Filing Language: (26) Publication Language: 21 November 2012 (21.11.2012) English (30) Priority Data: 61/562,094 21 November2011 (21.11.2011) English US (71) Applicant: UNIVERSITY HEALTH NETWORK [CA/CA]; 190 Elizabeth Street, R. Fraser Elliott Building - Room 1S-417, Toronto, Ontario M5G 2C4 (CA). (72) Inventors: KELLER, Gordon; #1 Douglas Crescent, Toronto, Ontario M4W 2E6 (CA). ZUNIGA-PFLUCK- ER, Juan Carlos; 787 Shaw Street, Toronto, Ontario M6G 3L9 (CA). KENNEDY, Marion J.; 1 Douglas Crescent, Toronto, Ontario M4W 2E6 (CA). STURGEON, Chris­ topher Michael; 1-28 Indian Road Crescent, Toronto, Ontario M6P 2G1 (CA). DITADI, Andrea; 2-400 Roxton Road, Toronto, Ontario M6G 3R2 (CA). AWONG, Geneve Sheandra; 33 Wood Street, Apt# 602, Toronto, Ontario M4Y 2P8 (CA). (74) Agents: DONAHUE, Brian, A. et al.; Morrison & Foer- ster LLP, 755 Page Mill Road, Palo Alto, CA 94304-1018 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (54) Title: POPULATIONS OF HEMATOPOIETIC PROGENITORS AND METHODS OF ENRICHING STEM CELLS THERE­ FOR CJ CJ CJ i> © en i-H o CJ O £ D Activin/Nodal Primitive Hematopoiesis CD341ow/- CD43+ CD34+ CD235a+ CD43+ C041a+ EfyP-GFC CFU-M primitive streak f mesoderm xi8y A. ?:»xJay~12: 5 < ^ o Ery-CFC CFU-M T cell Figure 7 Definitive Hematopoiesis (57) Abstract: There described herein method is a of enriching a population of stem cells for hematopoietic progenitors. The method comprises inducing hematopoietic differentiation in population of human a embryonic stem cells or human induced pluripotent stem cells; sorting the population based on expression of CD43 and at least one of CD34, CD31 and CD144; and selecting a fraction that is at least one of CD34 CD43\", CD31 CD43\" + + and CD144 CD43\". + Also provided are populations of hematopoietic progenitors ob­ tained by the methods described herein.