Patent SG-11201407809Q-A (SG)
Title: Chromatographic purification of immunoglobulin g preparations with particles having multimodal functionalities
Inventor: GAGNON PETER STANLEY
IPC: C07K1/16
Filed: 2013-01-21
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/180647 A1 (51) International Patent Classification: C07K1/16 (2006.01) C07K16/00 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000031 21 January 2013 (21.01.2013) English English (30) Priority Data: 61/653,913 31 May 2012 (31.05.2012) US (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionpolis Way, #20-10 Connexis, Singapore 138632 (SG). (72) Inventor: GAGNON, Peter, Stanley; 20 Biopolis Way, #06-01 Centres, Singapore 138668 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; 1531 Robinson Road Post Office, Singapore 903031 (SG). BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) (81) Designated States (unless otherwise indicated, for every Published: kind of national protection available)-. AE, AG, AL, AM, _ . ^ international search t (Art 21(3) ) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, F 1 1 r- •t o 00 i-H en i-H o CJ o & (54) Title: CHROMATOGRAPHIC PURIFICATION OF IMMUNOGLOBULIN G PREPARATIONS WITH PARTICLES HAV­ ING MULTIMODAL FUNCTIONALITIES (57) Abstract: A method of purifying sample a containing a desired protein includes the steps of (i) providing a packed chromato­ graphic column having positively charged porous particles, (ii) equilibrating the column to the conditions to which the desired pro­ tein in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume ap - plied to the packed chromatographic column is less than or equal to the interparticle space of the positively charged porous particles within the packed chromatographic column, (iv) eluting the desired protein from the packed chromatographic column, where the de­ sired protein is in a purer state and in the conditions to which the packed chromatographic column was equilibrated; where the de - sired protein is an antibody, an antibody fragment, an antibody derivative, or an antibody fusion protein. (No suitable Figure)