Patent SG-11201804381S-A (SG)
Title: De novo antibody design
Inventor: BAKER TERENCE SEWARD
IPC: G16B15/00
Filed: 2016-12-01
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property , Organization International Bureau ir s... 00)d (43) International Publication Date .... ....r .„,„1 8 June 2017 (08.06.2017) W I P0 1 PCT ID Hit (10) WO International 2017/093435 111111111111111111IIIIII11111111111111111111111111111111IIIIIMIIIIIIIIIIIIIII Publication Al Number (51) International Patent Classification: (74) Agent: FORSYTHE, Dominic; J A Kemp, 14 South GOOF 19/16 (2011.01) Square, Gray's Inn, London Greater London WC1R 5JJ (GB). (21) International Application Number: PCT/EP2016/079497 (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (22) International Filing Date: AO, AT, AU, AZ, BA, BB, BG, BH, BN BR, BW, BY, 1 December 2016 (01.12.2016) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (25) Filing Language: English DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (26) Publication Language: English KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (30) Priority Data: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, 1521447.1 4 December 2015 (04.12.2015) GB OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (71) Applicant: UCB BIOPHARMA SPRL [BE/BE]; Allee TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, de la Recherche 60, 1070 Brussels (BE). ZW. (72) Inventors: BAKER, Terence Seward; c/o UCB Celltech, (84) Designated States (unless otherwise indicated, for every 208 Bath Road, Slough Berkshire SL1 3WE (GB). LIU, kind of regional protection available): ARIPO (BW, GH, Xiaofeng; c/o UCB Celltech, 208 Bath Road, Slough GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Berkshire SL1 3WE (GB). SHI, Jiye; c/o UCB Celltech, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, 208 Bath Road, Slough Berkshire SL1 3WE (GB). TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, TAYLOR, Richard David; c/o UCB Celltech, 208 Bath DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Road, Slough Berkshire SL1 3WE LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (GB). SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). [Continued on next page] (54) Title: DE NOVO ANTIBODY DESIGN : designing (57) Computer-implemented methods of an antibody 1 that will bind to a target epitope are disclosed. In one arrangement, the meth- Fig. identifying hotspot that bind to od comprises one or more residues will each a corresponding one of one or more hotspot sites on the target epitope. Can- didate antibody structures are selected from a database such that characterist- ic atoms within the antibody structure and hotspot characteristic atoms can Identify hotspot 00 be superimposed computationally with an averaged spatial deviation less residue(s) than a predetermined threshold. A designed antibody is generated by repla- cing matching residues with different residues such that a predicted affinity is increased. Select candidate antibody structures from 200 database 1-1 • .4t in M it M Generate a designed antibody using candidate antibody structure 300 01 0 It-- 1-1 0 ei O WO 2017/093435 Al MIDEDIMO110101011101011110111100111110ME1110VOIMIE Published: — with international search report (Art. 21(3))