Patent SG-11201811149T-A (SG)
Title: METABOLIC DRUG LOADING OF EVs
Inventor: WIKLANDER Oscar
IPC: A61K35/545
Filed: 2017-07-10
Abstract: oo C-AraC endogenous EVs -0- C18-AraC exogenous EVs -o- Free C18-4raC 4. CTRL EVa 0.1 1 10 100 1000 Figure 1 Conc (nM) 120 100 ; 8 6 0 0 40 20 0 0.01 • (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 18 January 2018 (18.01.2018) W I PO I PCT omit °nolo omm HE om °nom° oimIE (10) International Publication Number WO 2018/011128 Al (51) International Patent Classification: A61K 9/48 (2006.01) A61K 35/545 (2015.01) A61K 48/00 (2006.01) A61K 47/66 (2017.01) A61K 35/13 (2015.01) (21) International Application Number: PCT/EP2017/067237 (22) International Filing Date: 10 July 2017 (10.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1611990.1 11 July 2016 (11.07.2016) GB (71) Applicant: EVOX THERAPEUTICS LTD [GB/GB]; 2nd Floor, King Charles House, Park End Street, Oxford, Oxfordshire OX1 1JD (GB). (72) Inventor: WIKLANDER, Oscar; Greta Garbos vag 21B, 16940 Solna (SE). (74) Agent: COOLEY (UK) LLP; Dashwood, 69 Old Broad Street, London, Greater London EC2M 1QS (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) (54) Title: METABOLIC DRUG LOADING OF EVs (57) : The present invention relates to methods for metabolically loading extracellular vesicles (EVs) with a pharmacological agent. The methods comprise culturing EV source cells in the presence of a metabolic component comprising a pharmacological agent, which is thereby incorporated into the EV-producing cells and subsequently into the EV. The invention further relates to medical uses and com- positions of such EVs. W O 20 18/0 11128 Al UT CTRL EVs Free PhLip-IBU PhLip-IBU endogenous EVs PhLip-IBU exogenous EVs Positive CTRL 0 20 40 60 80 100 120 Relative COX-2 activity (estimated based on PGE2 pg/ml) UT CTRL EVs IBU-B9 exogenous EVs- -1 IBU-B7 exogenous EVs--I IBU-B9 endogenous EVs-1 IBU-B7 endogenous EVs-M-1 Positive CTRL+ 20 40 60 80 100 120 Figure 3 (estimated based on PGE2 pg/ml) Relative COX-2 activity Figure 2