Patent SG-11201908360Q-A (SG)
Title: In-situ fluidic inspection
Inventor: LIN SZ-CHIN STEVEN
IPC: B01L3/00
Filed: 2018-04-02
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11 October 2018 (11.10.2018) WIPO I PCT ill~~~~~~~~ 011101010VIIIOH oI0 OH1110IIIIIDIDIIIIImmoiliollIE (10) International Publication Number WO 2018/187207 A3 (51) International Patent Classification: BOIL 3/00 (2006.01) B81C 99/00 (2010.01) (21) International Application Number: PCT/US2018/025686 (22) International Filing Date: 02 April 2018 (02.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/482,414 06 April 2017 (06.04.2017) US (71) Applicant: ILLUMINA, INC. [US/US]; 5200 Illumina Way, San Diego, California 92122 (US). (72) Inventors: LIN, Sz-Chin Steven; 5200 Illumina Way, San Diego, California 92122 (US). LEMOINE, Richard; 5200 Illumina Way, San Diego, California 92122 (US). PAN- CHAPAKESAN, Rajagopal; 5200 Illumina Way, San Diego, California 92122 (US). COX-MURANAMI, Wes- ley A.; 5200 Illumina Way, San Diego, California 92122 (US). SEGALE, Darren; 5200 Illumina Way, San Diego, California 92122 (US). (74) Agent: SCUDERI, Stephen P.; Heslin Rothenberg Farley & Mesiti P.C., 5 Columbia Circle, Albany, New York 12203 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: IN-SITU FLUIDIC INSPECTION FIG. 1B y100 (57) : A method includes engaging a well of a cartridge with a flow sensor of an instrument. The cartridge includes: a rotary valve including a rotatable port and a center port; the well in fluid communication with a channel, the channel including a channel port that the rotatable port is to align to in order to receive fluid from the well; and a flowcell including an inlet gasket in fluid communication with the center port. A source of pressurized air is connected to the flow sensor in order to establish a mass flow of air through a flow path. The flow path extends through one of the flow sensor, the channel, the rotary valve, and the flowcell. The mass flow of air through the flow path is measured with the flow sensor. It is determined if there is one of an air leak and an air blockage within the flow path. 10 I 56 [Continued on next page] W O 20 18/187 207 A3 WO 2018/187207 A3 MIDEDIMO 11111 OE IIMOIIMIEDMOHEIHMIHMOMECIE Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) (88) Date of publication of the international search report: 03 January 2019 (03.01.2019)