Patent SG-11201810332T-A (SG)
Title: Neoepitope vaccine compositions and methods of use thereof
Inventor: JONES FRANK
IPC: C07K14/705
Filed: 2017-05-26
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) CORRECTED VERSION ,,,,,,,,,1111.111.„ m„ Organization 11„ ,,,,,,„„,11,11„,,,,,,,,,,,,,111111111111111111111101111 (19) World Intellectual Property ---,. International Bureau (10) International Publication Number (43) International Publication Date ...../ WO 2017/205810 A8 30 November 2017 (30.11.2017) WIPO I PCT (51) International Patent Classification: SOON-SHIONG, Patrick; 9920 Jefferson Boulevard, Cul- A61K 39/00 (2006.01) C07K 14/705 (2006.01) ver City, CA 90232 (US). NIAZI, Kayvan; 9920 Jefferson C12N 15/86 (2006.01) Boulevard, Culver City, CA 90232 (US). RABIZADEH, (21) International Application Number: Shahrooz; 9920 Jefferson Boulevard, Culver City, CA PCT/US2017/034802 90232 (US). (22) International Filing Date: (74) Agent: RAMANATHAN, Renuka et al.; Wilson Sonsini 26 May 2017 (26.05.2017) Goodrich & Rosati, 650 Page Mill Road, Palo Alto, CA 94304-1050 (US). (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of national protection available): AE, AG, AL, AM, (30) Priority Data: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 62/342,752 27 May 2016 (27.05.2016) US CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (71) Applicants: ETUBICS CORPORATION [US/US]; 410 HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, West Harrison Street, Suite 200, Seattle, WA 98119 (US). KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, NANT HOLDINGS IP, LLC [US/US]; 9922 Jefferson MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, Boulevard, Culver City, CA 90232 (US). NANTCELL, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, INC. [US/US]; 9920 Jefferson Boulevard, Culver City, CA SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, = 90232 (US). TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (72) Inventors: JONES, Frank, R.; 410 West Harrison Street, (84) Designated States (unless otherwise indicated, for every = Suite 200, Seattle, WA 98119 (US). RICE, Adrian; 410 kind of regional protection available): ARIPO (BW, GH, — West Harrison Street, Suite 200, Seattle, WA 98119 (US). GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, = — (54) Title: NEOEPITOPE VACCINE COMPOSITIONS AND METHODS OF USE THEREOF (57) : In certain embodiments, methods and compositions are pro- _ _ = Patient Sample —* Whole exome sequencing for mutations vided for generating immune responses against tumor neo-antigens or neo- epitopes. In particular embodiments there may be provided methods for con- = strutting and producing recombinant adenovirus-based vector vaccines con- taining nucleic acid sequences encoding tumor neo-antigens and neo-epitopes = — — - = =f - Translation of mutated sequence with centrally positioned (and corresponding WT peptide) mutant into 17mer amino acid that allow for vaccinations in individuals with preexisting immunity to aden- ovirus. In additional embodiments, methods and compositions are provided for the treatment of cancer using immunotherapy based on recombinant ade- killer cells. In = MHC Class I prediction novirus-based vectors combined with engineered natural some binding Analysis embodiments, the methods and compositions further comprises a nucleic acid — encoding for an immunological fusion partner. = — = IEBD Analysis homology to known epitopes from of or cancers i T cell interaction prediction (IEBD program) GC Peptides predicted to bind MHC Class I with IC505500 (- Peptides predicted toy bind MHC Class I with 1050.500 , or interact with T cell, © 11 GC kr) © Candidate Neo-antlgens or Neo-epltopes r'l --.... IN FIG. 1 1-1 0 r'l C [Continued on next page] WO 2017/205810 A8 MIDEDIMOMOIDEIROIDEMODIMINHOMMOVE# UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) (48) Date of publication of this corrected version: 04 January 2018 (04.01.2018) (15) Information about Correction: see Notice of 04 January 2018 (04.01.2018)