Patent CA-3079574-A1 (CA)
Inventor: ALONSO FERNANDEZ MARIA JOSE
IPC: A61K31/337
Filed: 2018-11-02
Abstract: CA 03079574 2020-04-17 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111 1 11111111 111111 1 11 11111 1 111 11111 1 11 111111111 1111 1111 1 1111 11 1111111111 1 111111 Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2019/086627 Al 09 May 2019 (09.05.2019) WIPO I PCT (51) International Patent Classification: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, A 61K 9/51 (2006.01) A 61K 39/395 (2006.01) OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, A61K 31/337 (2006.01) SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (21) International Application Number: PCT/EP2018/080050 (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (22) International Filing Date: GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, 02 November 2018 (02.11.2018) UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (25) Filing Language: English TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, EE, IS, IT, LT, LU, LV, (26) Publication Language: English MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (30) Priority Data: TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, P201'7312'7'7 02 November 201'7 (02.11.201'7) ES KM, ML, MR, NE, SN, TD, TG). (71) Applicant: UNIVERSIDADE DE SANTIAGO DE Published: COMPOSTELA [ES/ES]; Edificio EMPRENDIA - Cam-¨ with international search report (Art. 21(3)) pus Vida, 15'782 Santiago de Compostela 15 A Conifia (ES). _ before the expiration of the time limit for amending the (72) Inventors: ALONSO FERNANDEZ, Maria José; claims and to be republished in the event of receipt of UNIVERSEDADE DE SANTIAGO DE COMPOSTELA amendments (Rule 48.2(h)) Edificio EMPRENDIA - Campus Vida, 15'782 SANTIA- ¨ with sequence listing part of description (Rule 5.2(a)) GO DE COMPOSTELA 15 A Corufia (ES). TEIJEIRO OSORIO, Desireé; UNIVERSIDADE DE SANTIAGO DE COMPOSTELA Edificio EMPRENDIA -Campus Vi-da, 15'782 SANTIAGO DE COMPOSTELA 15 A Corufia (ES). TEIJEIRO VALINO, Carmen Maria; UNIVERSI-_ DADE DE SANTIAGO DE COMPOSTELA Edificio EM-PRENDIA - Campus Vida, 15'782 Santiago de Compostela 15 A Conuia (ES). CADETE PIRES, Ana; UNIVERSI-DADE DE SANTIAGO DE COMPOSTELA Edificio EM-PRENDIA - Campus Vida, 15'782 Santiago de Compostela 15 A Corufia (ES). (74) Agent: ZBM PATENTS APS; Symbion, Box 33 Frueb-jergvej 3, 2100 Copenhagen (DK). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (54) Title: DRUG DELIVERY SYSTEMS AND METHODS COMPRISING POLYSIALIC ACID AND/OR OTHER POLYMERS ¨ (57) Abstract: The present invention generally relates to particles, including nanocapsules or other nanoentities, comprising a polymer It such as polysialic acid. The particles are able to access the interior of the cells, and/or to procure the intracellular release of the associated "I'IG drugs. In 5 one aspect, the present invention is directed to nanocapsules or other entities having an exterior or surface comprising a h polymer such as polysialic acid. In some cases, targeting moieties such as Lyp-1 or tLyp-1 peptide are bonded to the polymer, e.g., 2 using aminoalkyl (CI-CO succinimide or other linkers. These may be created, for example, by reacting a carboxylate moiety on a polymer with an aminoalkyl maleimide (CI-CO or an aminoalkyl 10 (CI-CO methacrylamide, and reacting the resulting aminoalkyl Ole (CI-CO maleimide or the aminoalkyl (CI-CO methacyrlamide to a cysteine or other sulfur group. Targeting moieties are bonded to the polymer, for example, by reacting a carboxylate moiety on a polymer with a N-hydroxysuccinimide or a carbodiimide, and reacting the intermediate formed with a lysine or arginine group on a targeting peptide to produce polymer-amide-peptide. Other 15 aspects of the invention are generally directed to methods of making or using such compositions, kits including such compositions, or the like.