Patent SG-11201805533Q-A (SG)
Title: Composition and methods for cryopreservation of hutc
Inventor: GOSIEWSKA ANNA
IPC: A01N1/02
Filed: 2017-01-06
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization 11M111110111011111111111 010 11111 01110111 0111 010 11111 1111 10111111111111 0111111 International Bureau ... .... ..Yjd (10) International Publication Number (43) International Publication Date ..... ...r .....1 WO 2017/123465 Al 20 July 2017 (20.07.2017) WIPO I PCT (51) International Patent Classification: AO1N 1/02 (2006.01) (21) International Application (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/278,780 14 January (71) Applicant: DEPUY SYNTHES [US/US]; 325 Paramount 02767 (US). (72) Inventors: GOSIEWSKA, Skillman, New Jersey 08558 Welsh Road and McKean Pennsylvania 19477 (US). (74) Agents: SHIRTZ, Joseph DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, Number: MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, PCT/US2017/012491 NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, 6 January 2017 (06.01.2017) TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. English (84) Designated States (unless otherwise indicated, for every English kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 2016 (14.01.2016) US TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, PRODUCTS, INC. DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, Drive, Raynham, Massachusetts LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Anna; 199 Grandview Road GW, KM, ML, MR, NE, SN, TD, TG). , (US). KIHM, Anthony J.; Declarations under Rule 4.17: Road, Spring House, as to applicant's entitlement to apply for and be granted a patent (Rule 4. 17(ii)) F. et al.; Johnson & Johnson, as to the applicant's entitlement to claim the priority of the Plaza, New Brunswick, New Jer- earlier application (Rule 4. 17(iii)) Published: otherwise indicated, for every available): AE, AG, AL, AM, — with international search report (Art. 21(3)) BB, BG, BH, BN, BR, BW, BY, CR, CU, CZ, DE, DJ, DK, DM, One Johnson & Johnson sey 08933 (US). — (81) Designated States (unless kind of national protection AO, AT, AU, AZ, BA, BZ, CA, CH, CL, CN, CO, METHODS FOR CRYOPRESERVATION OF HUTC tissue-derived cells. The sucrose, mannitol, (54) Title: COMPOSITION AND 35 30 —30—FORMULATION B —.—FORMULATION I MOD, LOW Ca 25 20 ,,, — Figure 1 umbilical cord salts; and (d) DMSO, human umbilical cord tissue-derived cells LLI 0 151-- 11 10 kf) 1 1' Cr) N ,-1 N 1-1 (57) : This invention N cryopreservation compositions and lactobionic acid. The invention also provides for kits comprising the composition and 0 and methods of cryopreserving the cells, which utilize the compositions. ) C 0.5 1 15 2 2.5 3 3.5 4 45 TIME (hrs) provides for compositions for cryopreservation of human may comprise: (a) amino acids; (b) vitamins; (c) inorganic