Patent SG-11201807651T-A (SG)
Title: Marine dna polymerases
Inventor: LARSEN ATLE NORALF
IPC: C12N9/12
Filed: 2017-03-22
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/162765 Al 28 September 2017 (28.09.2017) WIPO I PCT (51) International Patent Classification: C12N 9/12 (2006.01) (21) International Application Number: PCT/EP2017/056865 (22) International Filing Date: 22 March 2017 (22.03.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 1604876.1 22 March 2016 (22.03.2016) GB (71) Applicant: UNIVERSITETET I TROMSO - NORGES ARKTISKE UNIVERSITET [NO/NO]; PO Box 6050, Langnes, N-9037 Tromso (NO). (72) Inventors: LARSEN, Atle Noralf; Sykehusveien 23, 9019 Tromso (NO). PIOTROWSKI, Yvonne; Sykehusveien 23, 9019 Tromso (NO). ASSEFA, Netsanet Gizaw; Syke- husveien 23, 9019 Tromso (NO). LANES, Olav; Sykehus- veien 23, 9019 Tromso (NO). (74) Agent: GARDNER, Rebecca; DEHNS, St Bride's House, 10 Salisbury Square, London, Greater London EC4Y 8JD (GB). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) = (54) Title: MARINE DNA POLYMERASES Figure 5 120 1rD Temperature eIC) (57) : The present invention relates to DNA polymerases. In particular, the present invention relates to DNA polymerases based on a DNA polymerase from a Psychrobacillus sp. The present invention provides an isolated DNA polymerase or an enzymat- ically active fragment thereof, said DNA polymerase comprising the amino acid sequence of SEQ ID NO:1 or comprising an amino acid sequence which is at least 70% identical to SEQ ID NO:l. The invention also provides nucleic acid molecules comprising a nucleotide sequence that encodes the DNA polymerase. The invention also provides a method of nucleotide polymerisation and a method of amplifying a nucleic acid in which the DNA polymerase or an enzymatically active fragment thereof is used. 111111111111110111011111111111010111110111011111111010111111111111111111111110111111 W O 20 17 / 16 27 65 Al