Patent SG-11201907469Y-A (SG)
Title: Method and cell line for production of polyketides in yeast
Inventor: MOOKERJEE SHOHAM
IPC: C12N1/19
Filed: 2018-02-19
Abstract: MalonyldoAFig. W O 20 18/ 1488 49 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 23 August 2018 (23.08.2018) WIP0 I PCT omit VIII °nolo VIII VIII 11E1E1m oimIE (10) International Publication Number WO 2018/148849 Al (51) International Patent Classification: C12N 15/52 (2006.01) Cl 2N 9/00 (2006.01) C12N 1/19 (2006.01) C12P 7/02 (2006.01) C12N 15/81 (2006.01) C12P 7/20 (2006.01) (21) International Application Number: PCT/CA2018/050190 (22) International Filing Date: 19 February 2018 (19.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/460,526 17 February 2017 (17.02.2017) US (71) Applicant: HYASYNTH BIOLOGICALS INC. [CA/CA]; 141 Avenue du President-Kennedy, Local SB-5230, Montreal, Quebec H2X 1Y4 (CA). (72) Inventors: MOOKERJEE, Shoham; c/o 141 Avenue du President-Kennedy, Local SB-5230, Montreal, Quebec H2X 1Y4 (CA). CAMPBELL, Alexander James; c/o 141 Avenue du President-Kennedy, Local SB-5230, Montreal, Quebec H2X 1Y4 (CA). WILTSHIRE, Zachary Douglas; c/o 141 Avenue du President-Kennedy, Local SB-5230, Montreal, Quebec H2X 1Y4 (CA). CHEN, Kevin John; c/ o 141 Avenue du President-Kennedy, Local SB-5230, Mon- treal, Quebec H2X 1Y4 (CA). (74) Agent: WOOD, David et al.; Borden Ladner Gervais LLP, 100 Queen Street, Suite 1300, Ottawa, Ontario K11 3 1J9 (CA). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) (54) Title: METHOD AND CELL LINE FOR PRODUCTION OF POLYKETIDES IN YEAST (57) : A method and cell line for producing polyketides in yeast. OH The method applies, and the cell line includes, a yeast cell transformed with a polyketide synthase coding sequence. The polyketide synthase enzyme catalyzes synthesis of olivetol or methyl-olivetol, and may in- HO dude Dictyostelium discoideum polyketide synthase (\"DiPKS\"). Wild Methyl Olio t' type DiPKS produces methyl-olivetol only. DiPKS may be modified to produce olivetol only or a mixture of both olivetol and methyl-olivetol. o o The yeast cell may be modified to include a phosphopantethienyl trans- HO J~~ s ,co A ferase for increased activity of DiPKS. The yeast cell may be modified to mitigate mitochondrial acetaldehyde catabolism for increasing mal- onyl-CoA available for synthesizing olivetol or methyl-olivetol.