Patent SG-11201900724W-A (SG)
Title: Methods for detecting mycobacteria with solvatochromic dye conjugates
Inventor: BERTOZZI CAROLYN
IPC: C12Q1/04
Filed: 2017-07-31
Abstract: Oco O C () INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 February 2018 (01.02.2018) WIP0 I PCT omit VIII °nolo III oim VIII oiliommo oimIE (10) International Publication Number WO 2018/023134 Al (51) International Patent Classification: C12Q 1/04 (2006.01) (21) International Application Number: PCT/US2017/044760 (22) International Filing Date: 31 July 2017 (31.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/368,928 29 July 2016 (29.07.2016) US (71) Applicant: THE BOARD OF TRUSTEES OF THE LE- LAND STANFORD JUNIOR UNIVERSITY [US/US]; Office Of The General Counsel, Building 170, 3rd Floor, Main Quad, P.O. Box 20386, Stanford, CA 94305-2038 (US). (72) Inventors: BERTOZZI, Carolyn, R.; Dept. of Chemistry, Stanford University, Stanford, CA 94305-4401 (US). KA- MARIZA, Mireille; Dept. of Chemistry, Stanford Univer- sity, Stanford, CA 94305-4401 (US). SHIEH, Peyton; 7601 Eads Avenue 10, La Jolla, CA 92037 (US). (74) Agent: PECK, John, W.; Kppb LLP, 2400 E. Katella, Suite 1050, Anaheim, CA 92806 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (54) Title: METHODS FOR DETECTING MYCOBACTERIA WITH SOLVATOCHROMIC DYE CONJUGATES Figure 1 (57) : A series of carbohydrate-dye conjugates, as well as a method for detection of pathogenic or other organisms (e.g., bacteria) using the same are provided. The carbohydrate-dye conjugate can be enzymatically incorporated into live and active (viable) bacteria of interest for facile detection of said bacteria. The conjugate incorporation is achieved by utilizing one or more of the enzymes that are endogenous to the bacteria of interest, which can incorporate the conjugate via the conjugate's carbohydrate. A detectable signal is produced by the conjugate's dye only upon incorporation into the bacteria of interest, due to the changes in the dye's local environment upon incorporation. The conjugate may be metabolically incorporated into the fatty outer membrane of a bacterial cell wall, which provides a distinctly hydrophobic environment for the conjugate's dye, causing it to produce a detectable signal. [Continued on next page] WO 2018/023134 Al MIDEDIMOMOIDEIREIO I 0 10 1101 VIII OMMIOMMEHOIS MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))