Patent EA-001201-B1 (EA)
Title: Antisecretory factor peptides regulating pathological permeability changes
Inventor: LONNROTH IVAR
IPC: A23K1/165
Filed: 1996-08-23
Abstract: 1. A recombinant antisecretory factor having essentially the amino acid sequence, SEQ ID No. 1, or homologues or fragments thereof, having essentially the same biological activity. 2. A fragment of the recombinant antisecretory factor according to claim 1, wherein comprising at least 7-8 amino and having essentially the same biological activity. 3. A fragment according to claim 2, wherein comprising amino acids nos. 35-42 of the amino acid sequence of the recombinant antisecretory factor of claim 1. 4. A fragment according to claim 2, wherein comprising amino acids nos. 35-46 of the amino acid sequence of the recombinant antisecretory factor of claim 1. 5. A fragment according to claim 2, wherein comprising amino acids nos. 35-51 of the amino acid sequence of the recombinant antisecretory factor of claim 1. 6. A fragment according to claim 2, wherein comprising amino acids nos. 35-80 of the amino acid sequence of the recombinant antisecretory factor of claim 1. 7. A fragment according to claim 2, wherein comprising amino acids nos. 1-80 of the amino acid sequence of the recombinant antisecretory factor of claim 1. 8. A peptide corresponding to a fragment of the recombinant antisecretory factor of claim 3 and having an amino acid sequense: X1VCX2X3KX4R wherein X1 is none or Ile, X2 is His, Arg, or Lys X3 is Ser, Leu or another neutral amino acid and X4 is Thr or Ala. 9. A composition for normalising pathological fluid transport and/or inflammatory reactions in animals including human comprising as an active principal an effective amount of the recombinant antisecretory factor or a homologue thereof of claim 3 or a fragment thereof of claims 2 to7. 10. Use of a recombinant antisecretory factor of claim 1 for manufacturing a composition of claim 9. 11. Use of a recombinant antisecretory factor of any claim 2 to 7 for manufacturing a composition of claim 9. 12. A process of normalising pathological fluid transport and/or inflammatory reactions in vertebrates, including human, wherein comprising administration to the animals including human an effective amount of a recombinant antisecretory factor or a homologue thereof of claim 1 or a fragment of claims 2 to 7. 13. A feed for normalising pathological fluid transport and/or inflammatory reactions in vertebrates, including human, wherein comprising as an active ingradient a recombinant antisecretory factor or a homologue thereof of claim 1 or a fragment of claims 2 to 7. 14. A polyclonal antibody, specific to the recombinant antisecretory factor or a homologue thereof of claim 1 or a fragment thereof of claims 2 to7. 15. Use of an antibody of claim 14 foe detecting the antisecretory factor or a homologue thereof of claim 1 or a fragment thereof of claims 2 to7. 16. A sequense of nucleic acid coding for the recombinant antisecretory factor or a homologue thereof of claim 1. 17. A sequense of nucleic acid coding for the recombinant antisecretory factor of claims 2 to 7. 18. Use of the nucleic acid of claim 16 for producing the recombinant antisecretory factor or a homologue thereof of claim 1. 19. Use of the nucleic acid of claim 17 for producing the recombinant antisecretory factor of claims 2 to 7. 20. Use of the sequense of the nucleic acid of claim 16 for a probe or a primer derived from said nucleic acid sequense, for detecting said nucleic acid sequense, coding for the recombinant secretory factor or a homologue thereof of claim 1 in vertebrates, including human. 21. Use of the sequense of the nucleic acid of claim 17 for a probe or a primer derived from said nucleic acid sequense, for detecting said nucleic acid sequense, coding for the recombinant secretory factor of claims 2 to 7. 22. Vector of expression comprising nucleic acid sequence of claim 16 or 17. 23. A strain of procaryotic cells transformed by the vector of claim 20. 24. A strain of eucaryotic cells except for human cells, transformed by the vector of claim 20. 25. A strain of procalyotic cells, capable to produce the recombinant antisecretory factor or a homologue of claim 1 or a fragment of recombinant antisecretory factor of claim 2 to 7. 26. A strain of eucaryotic cells, capable to produce the recombinant antisecretory factor or a homologue of claim 1 or a fragment of recombinant antisecretory factor of claim 2 to 7.