Patent NZ-514619-A (NZ)
Title: HIV peptides, antigens, vaccine compositions, immunoassay kit and a method of detecting antibodies induced by HIV
Inventor: SOERENSEN BIRGER
IPC: G01N33/569
Filed: 2000-03-02
Abstract: 1. Peptide c h a r a c t e r i z e d i n t h a t it comprises at least one amino acid sequence selected from the groups of amino acid sequences: Xaa, Xaa2Xaa3Xaa4 XaaS Xaa6 A) a Xaag Xaag Gh Thr Pro Trp Xaa, 4Xaa, s Xaa, 6 Xaa, 7 Xaa, $ Val Xaazo (SEQ ID NO: 1) wherein the amino acids of the chain could have the following meanings; Xaa in position 1 of the peptide derivate is Lys or Arg, Xaa in position 2 is Ala, Gly, Ser or Arg, Xaa in position 3 is Leu or Met, Xaa in position 4 is Gly or Arg, Xaa in position 5 is Pro, Thr, Val, Ser, Gln or Ala, Xaa in position 6 is Gly, Ala, Lys, Arg, Gln or Glu, Xaa in position 8 is Thr or Ser, Xaa in position 9 is Leu or live, Xaa in position 14 is Thr, Ser or Val, Xaa in position 15 is Ala or Ser, Xaa in position 16 is Cys or Ser, Xaa in position 17 is Gln or Leu Xaa in position 18 is Gly, Glu or Arg, Xaa in position 20 is Gly or Arg, the peptide comprises at least nine consecutive amino acids of the sequence of SEQ ID NO: 1, Xaa, Xaa2 Xaa3 Xaa4 XaaS Gly Leu Asn Pro Leu Val [Gly] n Xaa, 2 Xaa, 3 Tyr Xaa, 5 Pro Xaal7Xaal, Ile Leu Xaa2, Xaa22 (SEQ ID NO: 4) wherein the amino acids of the chain have the following meaning; Xaa in position 1 is Arg, Lys, Asp or none Xaa in position 2 is Trp, Gly, Lys or Arg, Xaa in position 3 is Ile, Leu, Val or Met Xaa in position 4 is live, Val or Leu Xaa in position 5 Leu, Met, Val or Pro Xaa in position 12 is Arg, Lys Xaa in position 13 is Met or Leu, Xaa in position 15 is Ser, Cys or Gln, Xaa in position 17 is Thr, Val, lle, Ser or Ala, Xaa in position 18 is Ser, Gly or Thr, Xaa in position 21 is Asp, Glu, Cys or Gly, Xaa in position 22 is Gly or none wherein the sequence of SEQ ID NO: 4 comprises at least six consecutive amino acids and n = 0,1,2 or 3, Xaa, Xaa2Xaa3 Pro lie Pro Xaa7 Xaa8 Xaa9 Xaa, 0Xaa1, Xaa, 2 [Gly] n Xaa, 3 Xaa, 4Xaa, s Xaa, 6 Xaa, 7 Xaa, 8 Xaa, g Xaa20 Xaa2, Xaa22 Xaa23 Xaa24 (SEQ ID NO: 9) wherein Xaa in position 1 is Asn, Ser, Gly, His, Ala, Pro, Arg or none Xaa in position 2 is Asn, Ala or Lys Xaa in position 3 is Pro, Gln, Gly, Ile or Leu Xaa in position 7 is Val or Ala Xaa in position 8 is Gly or Lys Xaa in position 9 is Glu, Asp, Lys, Phe or Thr Xaa in position 10 is lle, Met, Val or Leu Xaa in position 11 is Tyr, Leu or none Xaa in position 12 is Ser or none Xaa in position 13 is Arg or none Xaa in position 14 is Asp, Arg, Trp, Ala or none Xaa in position 15 is lie or none Xaa in position 16 is Tyr or none Xaa in position 17 is Lys or Arg Xaa in position 18 is Arg, Lys or Asp Xaa in position 19 is Trp or Gly Xaa in position 20 is Ile, Met, Val, Gln or Ala Xaa in position 21 is Ile, Val or Ala Xaa in position 22 is Leu, Met or Val Xaa in position 23 is Gly or Cys Xaa in position 24 is Leu or none wherein the sequence of SEQ ID NO: 9 consists of at least six consecutive amino acids and n = 1,2 or 3, and Xaa, Xaa2 lle lle XaaS Xaa6 Xaa7 Xaa8 Xaa9 Leu Xaa" [Gly] n [Arg] m Xaa, 2 Xaa, 3 Xaa, 4 Xaa, SXaa, s Xaa"Xaa, $ Xaa, 9Xaazo Xaa2, Xaa2zXaa23 Xaa24 Xaa25 (SEQ ID NO: 15) wherein the Xaa in position 1 is Pro, Lys, Arg or none Xaa in position 2 is Glu, Arg, Phe or Lys Xaa in position 5 is Pro or Thr Xaa in position 6 is Met, Thr or Nleu Xaa in position 7 is Phe or Leu Xaa in position 8 is Ser, Thr, Ala or Met Xaa in position 9 is Ala, Glu or Leu Xaa in position 11 is Ser or none Xaa in position 12 is Ala, Arg or none Xaa in position 13 is Ile, Leu or none Xaa in position 14 is Ser, Ala, Leu or none Xaa in position 15 is Tyr, Glu or Asp Xaa in position 16 is Gly or Asp Xaa in position 17 is Ala or Leu Xaa in position 18 is Thr, Ile, Val, Leu or Asn, Xaa in position 19 is Pro, Thr or Ser Xaa in position 20 is Tyr, Phe, Nleu, His or Gln Xaa in position 21 is Asp, Asn, Leu or Ala Xaa in position 22 is Leu, Ile, Val or Asn Xaa in position 23 is Asn, Tyr, Cys or Gly Xaa in position 24 is Thr, Met, lie, Ala, Val or none Xaa in position 25 is Gly or none wherein the sequence of SEQ ID NO: 15 consists of at least six consecutive amino acids, n = 1,2 or 3 and m= 0,1,2 or 3 independent of each other, the terminal ends of the sequences may be free carboxyl-or amino groups, amides, acyls, acetyls or salts thereof, two or more of the Cys residues may form part of an intrachain-or interchain disulphide binding, a-S- (CH2) p-S- or a- (CH2) p-bridge wherein p = 1-8 optionally intervened by one or more heteroatoms such as O, N and S and/or the said peptide sequences are immobilized to a solid support.