Patent SG-11201901733P-A (SG)
Title: Measurement of protein expression using reagents with barcoded oligonucleotide sequences
Inventor: FAN CHRISTINA
IPC: G01N33/68
Filed: 2017-09-25
Abstract: PUBLISHEDUNDER THE PATENT COOPERATION TREATY (PCT) oimiolollm °nolo olum moll!!! ooluou () International Publication Number WO 2018/058073 A2 WIPO I PCT (12) INTERNATIONAL APPLICATION (19) World Intellectual Property Organization International Bureau (43) International Publication Date 29 March 2018 (29.03.2018) (51) International Patent Classification: GO1N 33/68 (2006.01) G01N 33/543 (2006.01) G01N 33/50 (2006.01) GOOF 19/00 (2018.01) C12Q 1/68 (2018.01) (21) International Application Number: PCT/US2017/053331 (22) International Filing Date: 25 September 2017 (25.09.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/399,795 26 September 2016 (26.09.2016) US 62/464,279 27 February 2017 (27.02.2017) US 62/515,952 06 June 2017 (06.06.2017) US (71) Applicants: CELLULAR RESEARCH, INC. [US/US]; 4040 Campbell Avenue, Suite 110, Menlo Park, CA 94025 (US). BECTON, DICKINSON AND COMPANY [US/US]; 1 Becton Drive, Franklin Lakes, NJ 07417 (US). (72) Inventors: FAN, Christina; 4040 Campbell Avenue, Suite 110, Menlo Park, CA 94025 (US). CHANG, Christina; 4040 Campbell Avenue, Suite 110, Menlo Park, CA 94025 (US). SHUM, Eleen; 4040 Campbell Avenue, Suite 110, Menlo Park, CA 94025 (US). ZOELLNER, Olaf; 4040 Campbell Avenue, Suite 110, Menlo Park, CA 94025 (US). BANSAL, Nidhanjali; 4040 Campbell Avenue, Suite 110, Menlo Park, CA 94025 (US). GHADIALI, James; 4040 Campbell Avenue, Suite 110, Menlo Park, CA 94025 (US). LAM, Gretchen, Yinbon; 4040 Campbell Avenue, Suite 110, Menlo Park, CA 94025 (US). JENSEN, Eric; 1 Becton Drive, Franklin Lakes, NJ 07417 (US). ROHRER, Jurg; 1 Becton Drive Franklin Lakes, Franklin Lakes, CA 07417 (US). GAYLORD, Brent; 1 Becton Drive, Franklin Lakes, CA 07417 (US). MARTIN, Jody; 1 Becton Drive, Franklin Lakes, CA 07417 (US). VIDAL, Jason, G.; 1 Becton Dri- ve, Franklin Lakes, NJ 07417 (US). = (54) Title: MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SE- QUENCES 505a 505b 505c 515 520 510 N O co O 00 O C 530 FIG. 5 (57) : Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide. [Continued on next page] WO 2018/058073 A2 IMIOHOIIIMIIMENH 0 OIREIHEOIKIIIIMMOIS (74) Agent: FULLER, Michael, L.; Knobbe, Martens, Olsen & Bear LLP, 2040 Main Street 14th Floor, Irvine, CA 92614 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: without international search report and to be republished upon receipt of that report (Rule 48.2(g)) with sequence listing part of description (Rule 5.2(a))