Patent SG-11201808573Y-A (SG)
Title: Nucleic acid stabilization reagent, kits, and methods of use thereof
Inventor: MCEWEN JASON M
IPC: C07H21/02
Filed: 2017-03-30
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization MD 1101111 II HEIN HO 11111 01E010 Hill 010111110111110 HOED International Bureau .. .... ..Yejd (10) International Publication Number (43) International Publication Date ..... ...r .....1 WO 2017/173105 Al 5 October 2017 (05.10.2017) IN I PO I P CT (51) International Patent Classification: (30) Priority Data: C07H 21/02 (2006.01) C07D 221/00 (2006.01) 62/316,514 31 March 2016 (31.03.2016) US C07H 21/04 (2006.01) CO7D 303/04 (2006.01) CO7D 209/00 (2006.01) CO7D 307/77 (2006.01) (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 Hoon Street, Suite 320, Emeryville, California 94608 CO7D 211/04 (2006.01) CO7D 321/00 (2006.01) rt CO7D 217/02 (2006.01) (US). (72) Inventor: MCEWEN, Jason M.; 5858 Horton Street, (21) International Application Number: PCT/US2017/025065 Suite 320, Emeryville, California 94608 (US). (22) International Filing Date: (74) Agents: MCNEILL, Rebecca M. et al.; McNeill Baur 30 March 2017 (30.03.2017) PLLC, 125 Cambridge Park Drive, Suite 301, Cambridge, Massachusetts 02140 (US). (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, [Continued on next page] (54) Title: NUCLEIC , r Co oeocs4 ACID STABILIZATION REAGENT, KITS, AND METHODS OF USE THEREOF (57) : Reagents for stabilizing the nucleic acids of a biological cell, PA: p compositions, kits and methods of use thereof are described. The stabilization = the nucleic acids within the biological for reagents may prepare cell storage and 4 6 ,189 preserve the representative population of the nucleic acids for later isolation and =Module P odue Other Modulus analysis. = 2 71A 100 - .r.O . 1 9 = = = * 1 = \ \_ . 114 1 110. r. xrar i s4 aca FIG. 1A = 102-, ' '-'''° 10 = ir - 20t pox Liu FIG. 1B = 200-, r f Il 214- kr) 214 © 11 en , ,js-214 's- 214 -214 Ir...' 212 14. j 1,- 11 IN IN FIG. IC 1-1 0 ei O WO 2017/173105 Al MIDEDIMOMOIDEIRMEMOMMEHOM010 IIMMOVOIMIE BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, RU, KZ, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, KG, LU, MZ, TJ, TM), European (AL, AT, BE, BG, CH, CY, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, CZ, IT, RS, SE, DE, LT, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, PT, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, SL, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). UG, GA, US, UZ, VC, VN, ZA, ZM, ZW. Published: (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, with international search report (Art. 21(3))