Patent AU-2013205647-B2 (AU)
Title: Chimeric factor VIII polypeptides and uses thereof
Inventor: CHHABRA EKTA SETH
IPC: C07K14/00
Filed: 2013-01-12
Granted: 2016-02-04
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/106787 A1 18 July 2013 (18.07.2013) W I PO I P CT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A61K 38/37(2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, PCT/US2013/021330 DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, 12 January 2013 (12.01.2013) KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (25) Filing Language: English NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, (26) Publication Language: English RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, (30) Priority Data: ZM, ZW. 61/586,099 12 January 2012 (12.01.2012) US 61/586,654 13 January 2012 (13.01.2012) US (84) Designated States (unless otherwise indicated, for every 61/667,901 3 July 2012 (03.07.2012) US kind of regional protection available): ARIPO (BW, GH, 61/734,954 7 December 2012 (07.12.2012) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (71) Applicant: BIOGEN IDEC MA INC. [US/US]; 14 Cam- TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, bridge Center, Cambridge, MA 02142 (US). EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (72) Inventors: CHHABRA, Ekta, Seth; 1550 Worcester Road MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (Unit 128), Framingham, MA 01702 (US). LIU, Tongyao; TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, 53 Buckman Drive, Lexington, MA 02421 (US). ML, MR, NE, SN, TD, TG). PETERS, Robert; 51 Newfield street, West Roxbury, MA Published: 02132 (US). - with international search report (Art. 21(3)) (74) Agents: STEFFE, Eric, K. et al.; Sterne Kessler Goldstein - with sequence listing part description (Rule 5.2(a)) & Fox PLLC, 1100 New York Avenue, N.W., Washington, DC 20005 (US). (54) Title: CHIMERIC FACTOR VIII POLYPEPTIDES AND USES THEREOF Figure 1: Different VWF Constructs A ;B D1 D2 D' D3D 1 C336A C379A 011 n a 004 E VWVF-006 7' J 012 VWF-008 (FL-VWF) VWF-031 (VWF-Fc) VWFB25B VWF-0 53 (57) Abstract: The present invention provides a VWF fragment comprising the D' domain and D3 domain of VWF, a chimeric pro tein comprising the VWF fragment and a heterologous moiety, or a chimeric protein comprising the VWF fragment and a FVIII pro tein and methods of using the same. A polypeptide chain comprising a VWF fragment of the invention binds to or is associated with f4 a polypeptide chain comprising a FVIII protein and the polypeptide chain comprising the VWF fragment can prevent or inhibit bind ing of endogenous VWF to the FVIII protein. By preventing or inhibiting binding of endogenous VWF to the FVIII, which is a half life limiting factor for FVIII, the VWF fragment can induce extension of half-life of the FVIII protein. The invention also includes nucleotides, vectors, host cells, methods of using the VWF fragment, or the chimeric proteins.