Patent SG-11201909619R-A (SG)
Title: A method and a device for quantifying living organisms and use of a device
Inventor: BLACKBURN NICHOLAS DAVID
IPC: C02F103/00
Filed: 2017-04-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 November 2018 (01.11.2018) WIPO I PCT omit °nolo m11°1111E11 mini° oimIE (10) International Publication Number WO 2018/196932 Al (51) International Patent Classification: GO1N 21/64 (2006.01) C12Q 1/02 (2006.01) G01N 33/18 (2006.01) C12Q 1/06 (2006.01) GO1N 15/14 (2006.01) CO2F 103/00 (2006.01) (21) International Application Number: PCT/DK2017/050131 (22) International Filing Date: 28 April 2017 (28.04.2017) (25) Filing Language: English (26) Publication Language: English (71) Applicant: MICROWISE APS [DK/DK]; Munkegardsvej 6 B, 3490 Kvistgard (DK). (72) Inventors: BLACKBURN, Nicholas David; Munkegardsvej 6 B, 3490 Kvistgard (DK). POULSEN, Per-Ove; Broveshojvej 19, Lille Heddinge, 4673 Rod- vig Stevns (DK). ENGELL-SORENSEN, Kirsten; Terp Skovvej 107 b, Hojbjerg 8270 (DK). HAECKY, Pia Bodil; Munkegardsvej 6 B, 3490 Kvistgard (DK). (74) Agent: PATENTGRUPPEN A/S; Aaboulevarden 31, 4th floor, DK-8000 Aarhus C (DK). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (54) Title: A METHOD AND A DEVICE FOR QUANTIFYING LIVING ORGANISMS AND USE OF A DEVICE 11,12,13,14,16 15 • -2,3 10 18 17 V 19 Fig. 1 (57) : Disclosed is a method for quantifying living organisms (1, 4) in a liquid sample (2), the method comprising the steps of • guiding the liquid sample (2) into a chamber (3), • analysing pictures of the sample (2) inside the chamber (3) to detect the number of organisms (4) moving by themselves in the sample, • illuminating the sample (2) with light in at least a part of the violet-blue spectrum while detecting the number of organisms (1) that are fluorescent in the sample inside the chamber (3), and • analysing pictures of the sample (2) inside the chamber (3) while illuminating the sample (2) with light in at least a part of the violet-blue spectrum to detect 00 the number of organisms (1, 4) that are both moving by themselves and fluorescent. A device (15) for quantifying living organisms O (1, 4) and use of a device (15) is also disclosed. C [Continued on next page] WO 2018/196932 Al MIDEDIMOMOIDEIREEMOMOHEIRIMOIHIMEMOIMIE GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3))