Patent SG-11201903913Q-A (SG)
Title: A method of detecting the presence of different target analytes and related kits thereof
Inventor: YING JACKIE Y
IPC: G01N33/532
Filed: 2017-12-26
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111111111111111111111111111111111111111111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/132062 Al 19 July 2018 (19.07.2018) WI P0 I P C T (51) International Patent Classification: (72) Inventors: YING, Jackie Y.; c/o Institute of Bioengi- G01N 33/532 (2006.01) B82Y 15/00 (2011.01) neering and Nanotechnology, 31 Biopolis Way, Singapore (21) International Application Number: 138669 (SG). ZHANG, Yi; c/o Institute of Bioengineering PCT/SG2017/050645 and Nanotechnology, 31 Biopolis Way, Singapore 138669 (SG). YU, Yue; c/o Institute of Bioengineering and Nan- (22) International Filing Date: otechnology, 31 Biopolis Way, Singapore 138669 (SG). 26 December 2017 (26.12.2017) (74) Agent: DONALDSON & BURKINSHAW LLP; 24 Raf- (25) Filing Language: English fles Place, #15-00 Clifford Centre, Singapore 048621 (SG). (26) Publication Language: English (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (30) Priority Data: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 10201700257X 12 January 2017 (12.01.2017) SG CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, #20-10 Connexis, Singapore 138632 (SG). KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (54) Title: A METHOD OF DETECTING THE PRESENCE OF DIFFERENT TARGET ANALYTES AND RELATED KITS THEREOF (A) 441 427 443 = 425 437 429 i 439 400 — 435 445 -- 405 ti 40 7 417 s 409 ..su. U123 431 413 401- --- ' 4, 421 433 — *--- 411 415 419 FIG. 4 1-1 ei (57) : This disclosure relates to a multiplex diagnostic method and related kits thereof for detecting the presence of different V::+ © target analytes in a sample, the method comprising providing the sample and a plurality of reporter particles such as gold nanoparticles N having co different colours to a substrate, the substrate having a assigned spatial regions; and determining, on the substrate, the presence M of a colour arising from the reporter particles in each of a assigned spatial regions on the substrate, wherein each of the assigned spatial 1-1 --.... regions on the substrate is configured to immobilize a target analyte that is different from the other assigned spatial regions, wherein Ot reporter particles of each different colour are configured to couple to a target analyte that is different from the reporter particles of 1-1 © the other different colours, and wherein a is an integer that is at least two and co is an integer that is at least two. In one embodiment, ei the reporter particles are configured to give rise to a secondary colour when reporter particles of two or more different colours are O [Continued on next page] WO 2018/132062 Al MUNRO DOI HONER 13 0111 001 010 1101 VIII iliEVOIS SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) — in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE colocalized in the same spatial region, wherein the secondary colour resulting from the colocalization of the reporter particles of two or more different colours from the co different colours is distinct from another secondary colour resulting from the colocalization of reporter particles of other combinations of colours from the co different colours.