Patent SG-11201903428X-A (SG)
Title: Pluripotent stem cell assay
Inventor: SLUKVIN IGOR
IPC: C12N5/00
Filed: 2017-11-15
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111111111111111111111111111111111111111111111111111111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/090084 Al 24 May 2018 (24.05.2018) W I PO I PCT (51) International Patent Classification: TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, C12N 5/00 (2006.01) C12Q 1/00 (2006.01) KM, ML, MR, NE, SN, TD, TG). C12N 5/0735 (2010.01) G01N 33/48 (2006.01) (21) International Application Number: Published: PCT/AU2017/051254 — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (22) International Filing Date: 15 November 2017 (15.11.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 2016904679 16 November 2016 (16.11.2016) AU (71) Applicant: CYNATA THERAPEUTICS LIMITED [AU/AU]; Level 3, 62 Lygon Street, Carlton, Victoria 3053 (AU). (72) Inventors: SLUKVIN, Igor; 1 Turnwood Circle, Verona, Wisconsin 53593 (US). HEI, Derek; 3221 Tanglewood Dr, Madison, Wisconsin 53719 (US). DRIER, Diana; 302 Glenway Street, Madison, Wisconsin 53705 (US). (74) Agent: GRIFFITH HACK; GPO Box 1285, Melbourne, Victoria 3001 (AU). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (81) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (84) ,_ 1 (54) Title: PLURIPOTENT STEM CELL ASSAY (57) : The invention relates to a method for detecting residual, undifferentiated pluripotent stem cells (PSCs) in a culture of 7r cells differentiated from PSCs, the method comprising: culturing the cells on a substrate coated with laminin-521 and E-cadherin in a = medium comprising a ROCK inhibitor; quantitating in the cultured cells expression of a marker of residual, undifferentiated PSCs; and 0 © comparing the marker expression in the cultured cells with the marker expression in a reference culture of cells comprising a known 01 proportion of PSCs, wherein lower marker expression in the culture of cells than marker expression in the reference culture of cells C -F.. :) , indicates absence of residual, undifferentiated PSCs in the cultured cells or presence of residual, undifferentiated PSCs in the cultured Ot cells at a proportion lower than the known proportion of PSCs in the reference culture of cells. The invention also relates to a method C 1-1 for manufacturing a therapeutic composition and a method for treating or preventing a condition in a subject. ei C