Patent SG-11201901628X-A (SG)
Title: Methods and compositions for spinal cord cells
Inventor: VATINE GAD
IPC: C12N5/0797
Filed: 2017-08-29
Abstract: Figure14 Neuroepithelia 'Fgrr Aod Stage iRSC MN precursors (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 March 2018 (08.03.2018) WIPO I PCT ill~~~~~~~~ 011101110VIIIOH olo olo Ho mil Immo ow (10) International Publication Number WO 2018/044934 Al (51) International Patent Classification: A61K 35/24 (2015.01) Cl 2M 3/00 (2006.01) A61K 35/30 (2015.01) Cl 2N 5/0797 (2010.01) (21) International Application Number: PCT/US2017/049193 (22) International Filing Date: 29 August 2017 (29.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/380,780 29 August 2016 (29.08.2016) US PCT/US2016/057724 19 October 2016 (19.10.2016) US 15/352,289 15 November 2016 (15.11.2016) US (71) Applicant: CEDARS-SINAI MEDICAL CENTER [US/US]; 8700 Beverly Blvd., Los Angeles, California 90048 (US). (72) Inventors: VATINE, Gad; 508 1/2 South Ogden Drive, Los Angeles, California 90026 (US). SANCES, Sam; 2021 Ocean Avenue, Apt. 223, Santa Monica, California 90405 (US). SVENDSEN, Clive; 15242 Friends Street, Pacifica Palisades, California 90272 (US). SAREEN, Dhruv; 19375 Crystal Ridge Lane, Porter Ranch, California 91326 (US). KERL, Alexis J.; 8700 Beverly Blvd., Los Angeles, Cali- fornia 90048 (US). (74) Agent: CHEN, Stephen, W. et al.; NIXON PEABODY LLP, 300 South Grand Avenue, Suite 4100, Los Angeles, California 90071-3151 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, = (54) Title: METHODS AND COMPOSITIONS FOR SPINAL CORD CELLS Seat Ink reils per well D30 * SLDM IMDWF12 (57) : Described here are systems and methods for deriving both spinal motor neurons and brain microvascular endothelial cells from induced pluripotent stem cells using distinct methods and combining them in a chip format. Neurons cultured alone in chip microvolume displayed increased calcium transient function and chip-specific gene expression. When seeded with endothelial cells, interaction further enhanced neural function, elicited vascular-neural interaction, niche gene expression with enhanced in vivo-like signatures arising from the chip co-cultures. Development of novel media formulations further allow for improved readout of differen- tiation process, by eliminating additives that otherwise confound differentiation processes and resulting phenotypes. [Continued on next page] WO 2018/044934 Al MIDEDIMOMOIDEIREIDIONOMOINOMMEMOIMIE DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))