Patent SG-11201903411Y-A (SG)
Title: Anti-p53 antibodies
Inventor: SABAPATHY TR KANAGA
IPC: A61K38/17
Filed: 2017-10-17
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property MD 1101111 0 DOI HIM 011101 0 0111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/074978 Al 26 April 2018 (26.04.2018) WIP0 I PCT _ (51) (21) (22) (25) Filing Language: (26) (30) (71) International Patent Classification: (SG). LANE, David P; c/o Agency for Science, Technol- C07K16/18 (2006.01) A61P 35/00 (2006.01) ogy and Research, 1 Fusionopolis Way, #20-10 Connexis, A61K 39/395 (2006.01) A61K 38/17 (2006.01) Singapore 138632 (SG). HWANG, Le-Ann; c/o Agency A61K 48/00 (2006.01) GO1N 33/577 (2006.01) for Science, Technology and Research, 1 Fusionopolis Way, International Application Number: #20-10 Connexis, Singapore 138632 (SG). KOH, Xin Yu; c/o Agency for Science, Technology and Research, 1 Fu- PCT/SG2017/050522 sionopolis Way, #20-10 Connexis, Singapore 138632 (SG). International Filing Date: WAH, Liew Oi; c/o National University of Singapore, 11 17 October 2017 (17.10.2017) Hospital Drive, Singapore 169610 (SG). English (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore Publication Language: English 903031 (SG). Priority Data: (81) Designated States (unless otherwise indicated, for every 1617564.8 17 October 2016 (17.10.2016) GB kind of national protection available): AE, AG, AL, AM, Applicants: AGENCY FOR SCIENCE, TECHNOLO- AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, #20-10 Connexis North Tower, Singapore 138632 (SG). DZ, EC, EE, EG, ES, FL GB, GD, GE, GH, GM, GT, HN, SINGAPORE HEALTH SERVICES PTE LTD [SG/ ]; HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 31 Third Hospital Avenue, #03-03, Bowyer Block C, Singa- KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, pore 168753 (SG). NATIONAL UNIVERSITY OF SIN- MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, GAPORE [SG/ ]; 21 Lower Kent Ridge Road, Singapore OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, 119077 (SG). SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Inventors: SABAPATHY, Tr Kanaga; c/o National Can- cer Centre Singapore, 11 Hospital Drive, Singapore 169610 (72) — = — = = Title: ANTI-P53 ANTIBODIES R175H (57) : The invention relates to methods for producing an antibody which is specific for a mutant p53 polypeptide over wildtype p53 polypeptide, comprising using said mutant p53 polypeptide as an immunogen wherein said polypeptide comprises: (i) an antigen sequence, E comprising an amino acid sequence of the mutant p53 polypeptide including the mutation and (54) = = — , , N. at least one amino acid immediately adjacent to the mutation, and (ii) a scaffold sequence for providing the antigen sequence in a solvent-accessible configuration, in particular wherein ‘ I I \"\" the scaffold sequence is thioredoxin. In the specific embodiments, antibodies against mutant p53 comprising R175H, R248Q or R273H are generated. Also disclosed are the uses of said antibodies for diagnosis, prognosis and stratification of patient groups and further encompasses R248Q the use of antibodies for imaging and treatment of cancer. = = = _ _ = = = = = ,N.e,s R273H ,1 Il GC IN 71' 0 66 Il © ei ,g,,LE(EF_rANLA , BJFIZT , Iilf P C [Continued on next page] WO 2018/074978 Al MIDEDIMOMOIDEIREEMOOVEHROMEMOVOIMIE (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) — with (an) indication(s) in relation to deposited biological material furnished under Rule 13bis separately from the description (Rules 13bis.4(d)(i) and 48.2 (a) (viii))