Patent GB-682801-A (GB)
Title: Medicinal preparations obtained from yeast and congenital animal organs or tissues and a method of producing the same
Filed: 1950-05-18
Abstract: Amino-acid mixtures or preparations of intensified anti-an mic activity are obtained by simultaneously mutually autolysing yeast in admixture with titurated congenital animal organs or tissues or press juices obtained therefrom.  Liver in the preferred animal material. The autolysis may be effected in the presence of agents such as toluene, chloroform or ethyl acetate.  After the autolysis there may be added to the liquid mixture, preferably at a pH of 3 to 6, a solvent such as methanol, ethanol, propanol, glycerin or glycol capable of dissolving the anti-an mic factors and other active substances and amino-acids leaving proteins, polypeptides and carbohydrates undissolved. The extract may be concentrated in vacuum and the solvent distilled off to form a concentrated powder. Alternatively, the autolysate may be treated with an adsorbant such as fullers earth or active carbon, the adsorbate being treated with acids such as hydrochloric, sulphuric, or phosphoric, aqueous phenol, or soap solution to recover the vitamins and other active materials. The filtrate from the adsorption step contains the amino-acids and may be passed through several absorption columns containing ion exchange material such as ion exchange resins.  The first layers may be capable of adsorbing basic, aromatic and sulphurous amino acids, the following layers capable of adsorbing neutral amino acids and the last layers capable of adsorbing acetous amino-acids, whereupon the adsorbed amino-acids are eluted from the ion exchange material and worked up to various amino-acids.ALSO:Amino-acid mixtures or preparations of intensified anti-an mic activity are obtained by simultaneously mutually autolysing yeast in admixture with titurated congenital animal organs or tissues or press juices obtained therefrom.  Liver is the preferred animal material.  The autolysis may be effected in the presence of agents such as toluene, chloroform or ethyl acetate.  After the autolysis there may be added to the liquid mixture, preferably at a pH of 3 to 6, a solvent such as methanol, ethanol, propanol, glycerin or glycol capable of dissolving the antian  factors and other active substances and amino-acids leaving proteins, polypeptides and carbohydrates undissolved.  The extract may be concentrated in vacuum and the solvent distilled off to form a concentrated powder.  This may be converted into tablets or pills.  Alternatively, the autolysate may be treated with an adsorbent such as fuller's earth or active carbon, the adsorbate being treated with acids such as hydrochloric, sulphuric, or phosphoric, aqueous phenol, or soap solution to recover the vitamins and other active materials.  The filtrate from the adsorption step contains the amino-acids and may be passed through several absorption columns containing ion exchange material such as ion exchange resins. The first layers may be capable of adsorbing basic, aromatic and sulphurous amino acids, the following layers capable of adsorbing neutral amino acids and the last layers capable of adsorbing acetous amino-acids, whereupon the adsorbed amino-acids are eluted from the ion exchange material and worked up to various amino-acids.