Patent SG-11201903750R-A (SG)
Title: Improved sensing for automated biological cell injection
Inventor: BANERJEE ARUNAVA STEVEN
IPC: C12N15/89
Filed: 2017-10-31
Abstract: b6b 8b1 8b2 8b3 -5a1 8a2 O cc O cc O O (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 03 May 2018 (03.05.2018) WIP0 I PCT omit VIII °nolo omm moiiiimonno mil (10) International Publication Number WO 2018/080325 Al (51) International Patent Classification: C12N 15/89 (2006.01) B25J 7/00 (2006.01) B81B 7/00 (2006.01) (21) International Application Number: PCT/NZ2017/050141 (22) International Filing Date: 31 October 2017 (31.10.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 2016904438 31 October 2016 (31.10.2016) AU (71) Applicant: MEKONOS LIMITED [NZ/NZ]; 101 Pack- enham St, W., Auckland, 1010 (NZ). (72) Inventor: BANERJEE, Arunava Steven; Skydeck, 2150 Shattuck Avenue, Berkeley, California 94704 (US). (74) Agent: AJ PARK; State Insurance Tower, 1 Willis Street, P.O. Box 949, Wellington 6140 (NZ). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (54) Title: IMPROVED SENSING FOR AUTOMATED BIOLOGICAL CELL INJECTION Figure 1 (57) : A method of controlling a needle actuator to interact with a cell is provided, the method comprising: providing an actuator comprising a tower, a stage and a needle, wherein the needle is mounted on the stage; applying an electrostatic potential between the tower and the stage to retract the needle; moving the actuator towards the cell; reducing the potential so as to allow the stage and needle to move towards the cell; applying calibration data to detect when the needle has pierced the cell; and reducing the potential further once it has been detected that the needle has pierced the cell. The cell can be a biological cell. The needle can be a micro-needle and the stage can be a micro-stage. [Continued on next page] WO 2018/080325 Al MIDEDIMOMMIDIREIDINIRMOIMIIMOMMEMOIS MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))