Patent DE-102010007548-A1 (DE)
Title: Microarray consisting of spots, at which mixture of polymerase and sequence specific primers is immobilized and coated with substrate, in which enzyme enabling sequence specific amplification is added, useful to genotype and quantify DNA
Inventor: PRAUSE STEFAN
IPC: C07H21/00
Filed: 2010-02-11
Abstract: Microarray consisting of spots, at which a mixture of polymerase and sequence-specific primers is immobilized using a method and is additionally coated with a precipitation efficient substrate, in which an enzyme that enables a sequence-specific amplification e.g. MutS is added, is claimed. An independent claim is included for sequence-specific primers for recombinase polymerase amplification (RPA)-method, comprising 2A wild type sequences comprising: (a1) 2A wild type sequences comprising Cys-Ala-Gly-Ala-Cys-Ala-Ala-Cys-Gly-Thr-Ala-Ala-Gly-Thr-Gly-Thr-Gly-Ala-Thr-Thr-Thr-Ala; (a2) 2A mutant type sequences comprising Cys-Ala-Gly-Ala-Cys-Ala-Ala-Cys-Ala-Thr-Ala-Ala-Gly-Thr-Gly-Thr-Gly-Ala-Thr-Thr-Thr; (a3) 3 wild type sequences Thr-Gly-Thr-Cys-Thr-Gly-Gly-Ala-Ala-Ala-Gly-Thr-Cys-Ala-Gly-Cys-Cys-Thr-Thr-Thr-Ala-Gly; (a4) 3 mutant type sequences comprising Thr-Thr-Gly-Thr-Cys-Thr-Gly-Ala-Ala-Ala-Gly-Thr-Cys-Ala-Gly-Cys-Cys-Thr-Thr-Thr-Thr-Gly-Thr-Cys-Thr-Gly-Ala-Ala-Ala-Gly-Thr-Cys-Ala-Gly-Cys-Cys-Thr; (b1) 4 wild type sequences comprising Cys-Cys-Ala-Thr-Thr-Thr-Cys-Thr-Ala-Cys-Ala-Cys-Thr-Ala-Ala-Thr-Gly-Thr-Cys-Cys-Ala-Ala-Thr-Thr-Thr-Cys-Thr-Ala-Cys-Ala-Cys-Thr-Ala-Ala-Thr-Gly-Thr-Cys-Cys-Ala-Cys; (b2) 4 mutant type sequences comprising Cys-Ala-Thr-Thr-Ala-Ala-Thr-Gly-Thr-Ala-Gly-Ala-Ala-Ala-Thr-Gly-Gly-Cys-Cys-Gly; (b3) 5 wild type sequences comprising Cys-Ala-Ala-Ala-Ala-Gly-Gly-Ala-Thr-Thr-Thr-Ala-Thr-Ala-Ala-Ala-Cys-Thr-Thr-Cys-Ala-Ala-Thr-Cys-Cys-Gly-Ala-Cys-Cys-Ala-Ala-Ala-Ala-Gly-Gly-Ala-Thr-Thr-Thr-Ala-Thr-Ala-Ala-Ala-Cys-Thr-Thr-Cys-Ala-Ala-Thr-Cys; (b4) 5 mutant type sequences comprising Cys-Ala-Ala-Ala-Ala-Gly-Gly-Ala-Thr-Thr-Thr-Ala-Cys-Ala-Ala-Ala-Cys-Thr-Thr-Cys-Ala-Ala-Thr; (c1) 6 wild type sequences comprising Ala-Ala-Thr-Gly-Gly-Cys-Gly-Thr-Thr-Ala-Cys-Ala-Gly-Cys-Cys-Ala-Cys-Cys-Ala-Ala-Ala-Ala-Thr-Gly-Gly-Cys-Gly-Thr-Thr-Ala-Cys-Ala-Gly-Cys-Cys-Ala-Cys-Cys-Ala; (c2) 6 mutant type sequences comprising Ala-Thr-Gly-Gly-Cys-Ala-Thr-Thr-Ala-Cys-Ala-Gly-Cys-Cys-Ala-Cys-Cys-Ala-Ala-Cys; (c3) 7 wild type sequences comprising Gly-Cys-Ala-Ala-Thr-Ala-Cys-Thr-Thr-Gly-Thr-Gly-Ala-Thr-Gly-Ala-Ala-Thr-Gly-Ala-Thr-Thr-Thr-Ala-Ala-Thr-Ala-Thr; (c4) 7 mutant type sequences comprising Thr-Thr-Gly-Cys-Ala-Ala-Thr-Ala-Cys-Thr-Thr-Gly-Thr-Gly-Ala-Thr-Gly-Ala-Thr-Thr-Thr-Ala-Ala-Thr-Ala-Thr-Cys-Ala-Ala-Gly; (d1) 8 wild type sequences comprising Cys-Ala-Gly-Cys-Cys-Gly-Gly-Ala-Ala-Cys-Thr-Gly-Ala-Gys-Gys-Ala-Ala-Thr-Thr-Thr-Cys-Ala-Cys-Ala-Gly-Cys-Cys-Gly-Gly-Ala-Ala-Cys-Thr-GGly-Ala-Gly-Gly-Ala-Ala-Thr-Thr-Thr-Cys; (d2) 8 mutant type sequences comprising Cys-Ala-Gly-Cys-Cys-Ala-Gly-Ala-Ala-Cys-Thr-Gly-Ala-Gly-Gly-Ala-Ala-Thr-Thr-Thr-Cys; (d3) 10 wild type sequences comprising Ala-Thr-Ala-Gly-Gly-Gly-Cys-Ala-Ala-Ala-Cys-Ala-Cys-Thr-Gly-Ala-Gly-Ala-Cys-Cys-Ala-Ala-Thr-Ala-Gly-Gly-Gly-Cys-Ala-Ala-Ala-Cys-Ala-Cys-Thr-Gly-Ala-Gly-Ala-Cys; (d4) 10 mutant type sequences comprising Ala-Ala-Thr-Ala-Gly-Gly-Gly-Ala-Ala-Ala-Ala-Ala-Cys-Thr-Gly-Ala-Gly-Ala-Cys-Ala-Cys-Ala-Thr-Ala-Gly-Gly-Gly-Cys-Ala-Ala-Ala-Ala-Ala-Cys-Thr-Gly-Ala-Gly-Ala-Cys-Ala-Cys-Ala; (e1) 13 wild type sequences comprising Thr-Cys-Thr-Thr-Cys-Gly-Ala-Ala-Thr-Cys-Ala-Thr-Thr-Gly-Ala-Thr-Gly-Thr-Gly; (e2) 13 mutant type sequences comprising Ala-Ala-Thr-Gly-Ala-Gly-Thr-Cys-Gly-Ala-Ala-Gly-Ala-Gly-Cys-Thr-Thr-Thr-Thr-Gly-Thr-Cys-Thr-Thr-Cys-Gly-Ala-Cys-Thr-Cys-Ala-Thr-Thr-Gly-Ala-Thr-Gly-Thr-Gly; (e3) A551T wild type sequences comprising Thr-Gly-Gly-Thr-Cys-Thr-Thr-Gly-Cys-Thr-Ala-Gly-Cys-Gly-Cys-Ala-Ala-Cys-Thr-Cys-Cys-Ala-Thr-Thr-Thr-Gly-Gly-Thr-Gly-Thr-Thr-Gly-Cys-Thr-Ala-Gly-Cys-Gly-Cys-Ala-Ala-Cys-Thr-Cys-Cys; (e4) A551T mutant type sequences comprising Thr-Gly-Gly-Thr-Cys-Thr-Thr-Gly-Cys-Thr-Ala-Gly-Cys-Ala-Cys-Ala-Ala-Cys-Thr; (f1) M166C wild type sequences comprising Thr-Ala-Thr-Thr-Cys-Ala-Ala-Ala-Gly-Cys-Ala-Ala-Thr-Gly-Ala-Gly-Thr-Ala-Thr-Cys-Cys-Cys-Thr-Ala-Thr-Thr-Cys-Ala-Ala-Ala-Gly-Cys-Ala-Ala-Thr-Gly-Ala-Gly-Thr-Ala-Thr-Cys; (f2) M166V mutant type sequences comprising Thr-Ala-Thr-Thr-Cys-Ala-Ala-Ala-Gly-Cys-Ala-Gly-Thr-Gly-Ala-Gly-Thr-Ala-Thr-Cys; (f3) D949V wild type sequences comprising Thr-Ala-Cys-Ala-Cys-Ala-Thr-Thr-Thr-Cys-Thr-Thr-Cys-Ala-Thr-Cys-Ala-Ala-Thr-Cys-Ala-Thr-Ala; and (f4) D949V mutant type sequences comprising Gly-Cys-Thr-Ala-Thr-Gly-Ala-Thr-Thr-Gly-Thr-Thr-Gly-Ala-Ala-Gly-Ala-Ala-Ala-Thr-Gly.