Patent SG-11201906397U-A (SG)
Title: Diagnostic applications using nucleic acid fragments
Inventor: LO YUK-MING DENNIS
IPC: C12Q1/68
Filed: 2018-01-25
Abstract: FIGz a co C3 C t). ea 0 1 .0 4 E . 13) 0 2 (o F- 1-1 kr) GC N 1-1 00 O 1-1 N C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 02 August 2018 (02.08.2018) WIP0 I PCT omit IIl °nolo milli mu ow (10) International Publication Number WO 2018/137685 Al (51) International Patent Classification: C12Q 1/68 (2018.01) G01N 33/50 (2006.01) C12Q 1/70 (2006.01) (21) International Application Number: PCT/CN2018/074138 (22) International Filing Date: 25 January 2018 (25.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/450,541 25 January 2017 (25.01.2017) US 62/507,154 16 May 2017 (16.05.2017) US PCT/US2017/058099 24 October 2017 (24.10.2017) US (71) Applicants: THE CHINESE UNIVERSITY OF HONG KONG [CN/CN]; Office of Research and Knowledge Transfer Services, Room 301, Pi Ch'iu Building, Shatin, New Territories, Hong Kong 999077 (CN). GRAIL, INC. [US/US]; 1525 O'Brien Dr., Menlo Park, CA California 94025 (US). (72) Inventors: LO, Yuk-Ming Dennis; 4th Floor, 7 King Tak Street, Homantin, Kowloon, Hong Kong 999077 (CN). CHIU, Rossa Wai Kwun; House 31, Double Haven, 52 Ma Lok Path, Shatin, New Territories, Hong Kong 999077 (CN). CHAN, Kwan Chee; Flat C, 15th Floor, Block 5, Peak One, Mei Tin Road, Shatin, New Territories, Hong Kong 999077 (CN). JIANG, Peiyong; Flat 7 on 1st Floor of Block B of Kwong Lam Court, Nos. 62-66 (No. 62) Siu Lek Yuen Road, Shatin, New Territories, Hong Kong (CN). LAM, Wai Kei; Flat B, 3/F, Block 14 Beacon Heights, 14 Lung Ping Road, Kowloon, Hong Kong (CN). (54) Title: DIAGNOSTIC APPLICATIONS USING NUCLEIC ACID FRAGMENTS (57) : Various embodiments are directed to applications (e.g., classification of biological samples) of the analysis of the count, the fragmentation patterns, and size of cell-free nucleic acids, e.g., plasma DNA and serum DNA, including nucleic acids from pathogens, such as viruses. Embodiments of one application can determine if a subject has a particular condition. For example, a method of present disclosure can determine if a subject has cancer or a tumor, or other pathology. Embodiments of another application can be used to assess the stage of a condition, or the progression of a condition over time. For example, a method of the present disclosure may be used to determine a stage of cancer in a subject, or the progression of cancer in a subject over time (e.g., using samples obtained from a subject at different times). [Continued on next page] WO 2018/137685 Al MUNRO 11111111111111111111111111311111 1111111111 HIM 110 111110111111111111 (74) Agent: INSIGHT INTELLECTUAL PROPERTY LIMITED; 19 A, Tower A, InDo Building, No. 48A Zhichun Road, Haidian District, Beijing 100098 (CN). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))