Patent SG-11201908891Y-A (SG)
Title: Perfusion medium
Inventor: OGAWA DAISIE
IPC: C12P21/08
Filed: 2018-03-27
Abstract: 1 2 3 5 Day 1000 - 900 800 - 700 • 600 - 500 •• 400 - 300 200 100 •• VCD (e 5ce lls /m L) 1-1 O GC 1-1 00 O 1-1 N C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 04 October 2018 (04.10.2018) W I PO I PCT ill Hu onus °nolo olommoillioliono oimIE (10) International Publication Number WO 2018/178063 Al (51) International Patent Classification: C12P 21/00 (2006.01) Cl 2P 21/08 (2006.01) (21) International Application Number: PCT/EP2018/057749 (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/479,414 31 March 2017 (31.03.2017) US (71) Applicant: BOEHRINGER INGELHEIM INTER- NATIONAL GMBH [DE/DE]; Binger Strasse 173, 55216 INGELHEIM AM RHEIN (DE). (72) Inventors: OGAWA, Daisie; Boehringer Ingelheim In- ternational GmbH, Corporate Patents, Binger Strasse 173, 55216 INGELHEIM AM RHEIN (DE). LIN, Hen- ry; Boehringer Ingelheim International GmbH, Corporate Patents, Binger Strasse 173, 55216 INGELHEIM AM RHEIN (DE). (74) Agent: SIMON, Elke, Anna Maria et al.; Boehringer Ingelheim International GmbH, Corporate Patents, Binger Strasse 173, 55216 Ingelheim Am Rhein (DE). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 27 March 2018 (27.03.2018) (81) English English (54) Title: PERFUSION MEDIUM Figure 3 (A) (57) : The invention relates to a method of culturing mammalian cells expressing a heterologous protein in a perfusion cell culture comprising adding iron and a retinoid to reduce wasteful cell bleed during production phase. The invention further relates to a serum-free perfusion medium comprising iron and a retinoid and its use for culturing cells in a perfusion culture during production phase or for reducing the cell bleed volume during production phase. [Continued on next page] WO 2018/178063 Al MIDEDIMOMMIDIREEMOMOMMONEHOMOVOIS TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) Published: — with international search report (Art. 21(3))