Patent AU-2013205064-B2 (AU)
Title: Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
Inventor: POLLNER REINHOLD B
IPC: C12Q1/68
Filed: 2013-04-13
Granted: 2015-07-30
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/184553 Al 12 December 2013 (12.12.2013) W I P 0 I P C T (51) International Patent Classification: (71) Applicant: GEN-PROBE INCORPORATED [US/US]; C12Q 1/70 (2006.01) 10210 Genetic Center Drive, San Diego, California 92121 (21) International Application Number: (US). PCT/US2013/043839 (72) Inventors: POLLNER, Reinhold B.; 5239 Kerwood (22) International Filing Date: Court, San Diego, California 92130 (US). LEE, Shy( Intenatona Fihng Date:3 un-Shyun; 12820 Caminito Beso, San Diego, California 3 June 2013 (03.06.2013) 92130 (US). (25) Filing Language: English (74) Agent: LANDES, Jeffrey E.; Gen-Probe Incorporated, (26) Publication Language: English 10210 Genetic Center Drive, San Diego, California 92121 (30) Priority Data: (US). 61/655,382 4 June 2012 (04.06.2012) US (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, [Continued on next page] (54) Title: COMPOSITIONS AND METHODS FOR AMPLIFYING AND CHARACTERIZING HCV NUCLEIC ACID GCCAGCCCCCTGATGGGGGCGACACTCCACCATGAATCACTCCCCTGTGA (57) Abstract: Disclosed are nucleic acid oligomers for amplifying one S_ GGAACTACTGTCTTCACGCAGAAAGCGTCTAGCCATGGCGTTAGTATGAG or more selected regions of HCV nucleic acid. Also disclosed are meth TGTCGTGCAGCCTCCAGGACCCCCCCTCCCGGGAGAGCCATAGTGGTCTG ods for specific amplification and characterization of HCV nucleic acid CGGAACCGGTGAGTACACCGGAATTGCCAGGACGACCGGGTCCTTTCTTG using the disclosed oligomers, as well as corresponding reaction mix GATAAACCCGCTCAATGCCTGGAGATTTGGGCGTGCCCCCGCAAGACTGC tures and kits. TAGCCGAGTAGTTGT GGGTCGCGAAAGGCCTTGTGGTACTGCCTGATAGG GTGCTTGCGAGTGCCCCGGGAGGTCTCGTAGACCGTGCACCATGAGCACG __ AATCCTAAACCTCAAAGAAAAACCAAACGTAACACCAACCGTCGCCCACA GGACGTCAAGTTCCCGGGTGGCGGTCAGATCGTTGGTGGAGTTTACTTGT TGCCGCGCAGGGGCCCTAGATTGGGTGTGCGCGCGACGAGGAAGACTTCC GAGCGGTCGCAACCTCGAGGTAGACGTCAGCCTATCCCCAAGGCACGTCG GCCCGAGGGCAGGACCTGGGCTCAGCCCGGGTACCCTTGGCCCCTCTATG GCAATGAGGGTTGCGGGTGGGCGGGATGGCTCCTGTCTCCCCGTGGCTCT CGGCCTAGCTGGGGCCCCACAGACCCCCGGCGTAGGTCGCGCAATTTGGG TAAGGTCATCGATACCCTTACGTGCGGCTTCGCCGACCTCATGGGGTACA TACCGCTCGTCGGCGCCCCTCTTGGAGGCGCTGCCAGGGCCCTGGCGCAT GGCGTCCGGGTTCTGGAAGACGGCGTGAACTATGCAACAGGGAACCTTCC TGGTTGCTCTTTCTCTATCTTCCTTCTGGCCCTGCTCTCTTGCCTGACTG TGCCCGCTTCAGCCTACCAAGTGCGCAATTCCTCGGGGCTTTACCATGTC ACCAATGATTGCCCTAACTCGAGTATTGTGTACGAGGCGGCCGATGCCAT CCTGCACACTCCGGGGTGTGTCCCTTGCGTTCGCGAGGGTAACGCCTCGA GGTGTTGGGTGGCGGTGACCCCCACGGTGGCCACCAGGGACGGCAAACTC CCCACAACGCAGCTTCGACGTCATATCGATCTGCTTGTCGGGAGCGCCAC _ CCTCTGCTCGGCCCTCTACGTGGGGGACCTGTGCGGGTCTGTCTTTCTTG TTGGTCAACTGTTTACCTTCTCTCCCAGGCGCCACTGGACGACGCAAGAC TGCAATTGTTCTATCTATCCCGGCCATATAACGGGTCATCGCATGGCATG GGATATGATGATGAACTGGTCCCCTACGGCAGCGTTGGTGGTAGCTCAGC TGCTCCGGATCCCACAAGCCATCATGGACATGATCGCTGGTGCTCACTGG _ GGAGTCCTGGCGGGCATAGCGTATTTCTCCATGGTGGGGAACTGGGCGAA GGTCCTGGTAGTGCTGCTGCTATTTGCCGGCGTCGACGCGGAAACCCACG TCACCGGGGGAAGTGCCGGCCGCACCACGGCTGGGCTTGTTGGTCTCCTT ACACCAGGCGCCAAGCAGAACATCCAACTGATCAACACCAACGGCAGTTG GCACATCAATAGCACGGCCTTGAACTGCAATGAAAGCCTTAACACCGGCT GGTTAGCAGGGCTCTTCTATCAGCACAAATTCAACTCTTCAGGCTGTCCT GAGAGGTTGGCCAGCTGCCGACGCCTTACCGATTTTGCCCAGGGCTGGGG TCCTATCAGTTATGCCAACGGAAGCGGCCTCGACGAACGCCCCTACTGCT GGCACTACCCTCCAAGACCT TGTGGCAT TGTGCCCGCAAAGAGCGTGTGT GGCCCGGTATATTGCTTCACTCCCAGCCCCGTGGTGGTGGGAACGACCGA Figure1A W O 2 0 13 /18 4 5 5 3 A 1 l l lll|||11111ll|||11ll III1||| 11||||||||||||||||||||||||||||||||||||||||||||||||| DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, GW, KM, ML, MR, NE, SN, TD, TG). TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, Published: ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every with international search report (Art. 2](3)) kind of regional protection available): ARIPO (BW, GH, - with sequence listing part of description (Rule 5.2(a)) GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ,