Patent SG-11201810527T-A (SG)
Title: Methods and apparatus that increase sequencing-by-binding efficiency
Inventor: STROMBERG SEAN
IPC: C12Q1/6832
Filed: 2018-04-25
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111110111111111011111111 01111111111111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/200709 Al 01 November 2018 (01.11.2018) WIPO I PCT (51) International Patent Classification: EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, C12Q 1/6832 (2018.01) C12Q 1/6869 (2018.01) MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (21) International Application Number: TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, PCT/US2018/029420 KM, ML, MR, NE, SN, TD, TG). (22) International Filing Date: Published: 25 April 2018 (25.04.2018) — with international search report (Art. 21(3)) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/489,610 25 April 2017 (25.04.2017) US 62/526,514 29 June 2017 (29.06.2017) US 15/712,632 22 September 2017 (22.09.2017) US 15/922,787 15 March 2018 (15.03.2018) US (71) Applicant: OMNIOME, INC. [US/US]; 10575 Roselle Street, San Diego, CA 92121 (US). (72) Inventors: STROMBERG, Sean; C/o Omniome, Inc., 10575 Roselle Street, San Diego, CA 92121 (US). VIE- CELL, John; C/o Omniome, Inc., 10575 Roselle Street, San Diego, CA 92121 (US). VIJAYAN, Kandaswamy; C/o Omniome, Inc., 10575 Roselle Street, San Diego, CA 92121 (US). OLIPHANT, Arnold; C/o Omniome, Inc., 10575 Roselle Street, San Diego, CA 92121 (US). Agent: TERRANOVA, Zachary, L. et al.; Mintz Levin (74) Cohn Ferris Glovsky and Popeo, P.C., One Financial Cen- ter, Boston, MA 02111 (US). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (81) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (84) = 1-1 (54) Title: METHODS AND APPARATUS THAT INCREASE SEQUENCING-BY-BINDING EFFICIENCY ON r..., (57) : A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid © with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates C:::: ) for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring ei --.... the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence GC of 1-1 ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that c;;;;) encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the ei next template position from an error in the b ase call. C