Patent SG-11201901135P-A (SG)
Title: Integrated microfluidic system for culturing and testing
Inventor: BIGLIARDI PAUL
IPC: G01N33/50
Filed: 2017-08-10
Abstract: WO 18/03095 8 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 15 February 2018 (15.02.2018) WIP0 I PCT IiiimmolioloiollmonoloollloomoinuioluovoimIE (10) International Publication Number WO 2018/030958 Al (51) International Patent Classification: BOIL 3/00 (2006.01) Cl 2N 5/07 (2010.01) C12M 3/00 (2006.01) G01N 33/50 (2006.01) (21) International Application Number: PCT/SG2017/050400 (22) International Filing Date: 10 August 2017 (10.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201606627Q 10 August 2016 (10.08.2016) SG (71) Applicant: 1. AGENCY FOR SCIENCE, TECHNOL- OGY AND RESEARCH [SG/SG]; 1 Fusionopolis WAY #20-10 Connexis, Singapore 138632 (SG). (72) Inventors: BIGLIARDI, Paul; c/o Institute Of Medical Biology, 8A Biomedical Grove, #06-06, Singapore 138648 (SG). WANG, Zhiping; c/o Singapore Institute Of Manu- facturing Technology, 71 Nanyang Dr, Singapore 038075 (SG). WU, Ruige; c/o Singapore Institute Of Manufactur- ing Technology, 71 Nanyang Dr, Singapore 038075 (SG). MASSIMO, Alberti; c/o Singapore Institute Of Manu- facturing Technology, 71 Nanyang Dr, Singapore 038075 (SG). WU, Bo; c/o Singapore Institute Of Manufactur- ing Technology, 71 Nanyang Dr, Singapore 038075 (SG). BIGLIARDI-QI, Mei; c/o Institute Of Medical Biology, 8A Biomedical Grove, #06-06, Singapore 138648 (SG). GOPU, Sriram; c/o Institute Of Medical Biology, 8A Biomedical Grove, #06-06, Singapore 138648 (SG). RA- MASAMY, Srinivas; c/o Institute Of Medical Biology, 8A Biomedical Grove, #06-06, Singapore 138648 (SG). DANCIK, Yuri Hebert; c/o Institute Of Medical Biology, 8A Biomedical Grove, #06-06, Singapore 138648 (SG). (74) Agent: CHIN, Dexter Kam; Horizon IP Pte. Ltd., 7500A Beach Road, #04-306/308, The Plaza, Singapore 199591 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (54) Title: INTEGRATED MICROFLUIDIC SYSTEM FOR CULTURING AND TESTING (57) : The present disclosure describes a microfluidic chip comprising at least one microfluidic unit having two fluidic compartments, such as an upper and a lower compartment, sep- arated by a permeable support, one or more access openings for the fluidic compartments, and a first and a second set of inlet and outlet channels which allow fluids to be perfused into the two compartments, respectively. The permeable support serves as a support for a first sample provided through the access opening. The access opening may be closed with a lid or accommodate an 640 inset. In a preferred embodiment, the microfluidic chip is used for culturing and in vitro testing an organotypic skin culture or ep- ithelial tissue. The present disclosure also describes an environ- mental control system for interfacing with a microfluidic chip and for controlling temperature and humidity. 650 620 Fig. 6b [Continued on next page] WO 2018/030958 Al MUNRO 111111111111111111111111111111111111 11111111111111 1111110 IIIIIIIIIIII GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))