Patent SG-11201810339W-A (SG)
Title: Glycoengineering of e-selectin ligands
Inventor: SACKSTEIN ROBERT
IPC: C12N5/0797
Filed: 2017-05-22
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111111111111111111111111111111111101111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2017/201537 Al 23 November 2017 (23.11.2017) WIPO I PCT (51) International Patent Classification: GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, A61K 35/12 (2015.01) C12N 5/0784 (2010.01) UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, C12N 5/077 (2010.01) C12N 5/0797 (2010.01) TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, C12N 5/078 (2010.01) EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (21) International Application Number: MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, PCT/US2017/033868 TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (22) International Filing Date: 22 May 2017 (22.05.2017) Published: — (25) Filing Language: English with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (26) Publication Language: English (30) Priority Data: 62/339,704 20 May 2016 (20.05.2016) US 62/354,350 24 June 2016 (24.06.2016) US (72) Inventor; and (71) Applicant: SACKSTEIN, Robert [US/US]; 26 Fox Run Drive, Sudbury, Massachusetts 01776 (US). (74) Agent: KNUDSEN, Geoffry; Bryan Cave LLP, 1290 Av- _ enue of the Americas, New York, New York 10104 (US). = (81) Designated States (unless otherwise indicated, for every = kind of national protection available): AE, AG, AL, AM, - AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, = DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, = KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, = = TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = = (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, = = _ (54) Title: GLYCOENGINEERING = = = FIG. 1A = = CD2 Il OF E-SELECTIN LIGANDS CD34 CD45 CD73 CM -taNa: • if.'„<%%, CD105 v41 CD106 CD166 10 , 3% IN en (57) : The present invention provides methods of enforcing expression of an E-selectin and/or L-selectin ligand on a surface ir) of a cell. Also provided are methods of enabling and/or increasing binding of a cell to E-selectin and/or L-selectin, methods of increas- inghoming and/or extravasation in a population of transplanted cells, methods of producing modified cells, including stem cells, for N transplanting into a subject, methods of treating or ameliorating the effects of a symptom, a disease or an injury in a subject, and meth- ods for inducing and/or enhancing homing of a population of cells to a therapeutic target in a subject. The invention further provides - pharmaceutical compositions comprising a population of cells produced by the methods of the invention and kits that include such C:::: ) compositions for treating or ameliorating the effects of a symptom, a disease or an injury in a subject. ei C