Patent FR-2965358-A1 (FR)
Title: Use of at least one amino acid sequence encoded by a nucleic acid sequence or of nucleic acid sequence, as a biomarker of a state of aged skin and/or signs of aging, optionally associated with dry skin
Inventor: DELATTRE CAROLINE
IPC: A61K8/60
Filed: 2010-09-24
Abstract: Use (i) of at least one amino acid sequence encoded by a nucleic acid sequence represented by SEQ ID NO: 1 (comprising a fully defined 333 amino acid sequence as given in the specification) or its an analogue or a fragment, or (ii) of nucleic acid sequence, as a biomarker of a state of aged skin and/or signs of aging, optionally associated with dry skin, is claimed. Independent claims are included for: (1) use of at least one amino acid sequence comprising SEQ ID NO: 12-20 (one of 9 fully defined 11-121 amino acid sequences (SEQ ID NO: 12-20) as given in the specification) or its analog with a sequence identity of at least 90% with the sequence and a biological activity of the same nature, or its fragment which comprises 3-48 amino acids of the sequence and which has a biological activity of the same nature, or at least one the nucleic acid sequence for screening active agents or physical treatments, which may modulate the activity, expression or maturation of the amino acid sequence or the nucleic acid sequence; (2) use of (i), (ii) or at least one agent modulating the activity, expression or maturation of the amino acid sequence or the nucleic acid sequence to prepare a multilayered epithelial cell model, preferably a reconstructed skin model; (3) a process of in vitro or ex vivo process to characterize a state of aged skin and/or signs of skin aging, optionally associated with dry skin comprising: carrying out, in a sample isolated from a skin, a measurement of qualitative or quantitative expression, maturation or activity of the amino acid sequence or of the nucleic acid sequence and comparing the measurement made in the step (a) to a reference measurement; (4) a process of in vitro or ex vivo screening of active agents or for physical treatment, which may modulate the activity, expression or maturation of the amino acid sequence comprising SEQ ID NO: 12-20 or the nucleic acid sequence comprising: placing the amino acid sequence or the nucleic acid sequence under conditions favorable to the activity, expression or maturation of the sequences; contacting the amino acid sequence or the nucleic acid sequence with at least one active agent to be tested, or exposing the amino acid sequence or nucleic acid sequence with a physical treatment to be tested; carrying out a qualitative or quantitative measurement of the expression, maturation or the activity of the amino acid sequence or the nucleic acid sequence; and comparing the measurement to a reference measurement; (5) a cosmetic process for preventing and/or treating an aged skin and/or signs of skin aging, optionally associated with skin dryness, comprising administering, to the individual, at least one composition comprising as active agent (i) or (ii); (6) a process for in vitro or ex vivo cosmetic process, to characterize the effectiveness of a cosmetic skin treatment in an individual, comprising: carrying out, before the implementation of cosmetic treatment, in a first isolated skin sample taken from the individual, isolated, at least a first qualitative or quantitative measurement of the expression, maturation or activity of at least one amino acid sequence or at least one nucleic acid sequence; carrying out, after the implementation of the cosmetic treatment, in isolated second skin sample taken from the individual, at least a second qualitative or quantitative measurement of the expression, maturation or activity of the amino acid sequence or the nucleic acid sequence, and comparing the first and second measures, preferably in order to deduce information relating to an effect of implementation of the cosmetic treatment; (7) a isolated peptide represented by a sequence of amino acid SEQ ID NO: 18 (comprising a fully defined a fully defined 29 amino acid sequence as given in the specification) or its analogue or its fragment; and (8) a cosmetic composition comprising a peptide or a nucleic acid sequence encoding the peptide. ACTIVITY : Dermatological; Antipruritic. MECHANISM OF ACTION : None given.