Patent SG-11201811332Y-A (SG)
Title: Reagent nozzle sipper mixing system and method
Inventor: DREWS BRADLEY KENT
IPC: G01N35/10
Filed: 2017-12-21
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111111111111111111111111111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ...... WO 2018/128842 Al 12 July 2018 (12.07.2018) WIP0 1 PCT (51) International Patent Classification: Stephen Wayne; 5200 Illumina Way, San Diego, Califor- GO/N 35/10 (2006.01) G01N 35/00 (2006.01) nia 92122 (US). WHITACRE, Johanna Lynn; 5200 Illu- BOIL 3/00 (2006.01) G01N 35/04 (2006.01) mina Way, San Diego, California 92122 (US). PHELPS, G01N 35/02 (2006.01) Steven Scott; 5200 Illumina Way, San Diego, California (21) International Application Number: 92122 (US). ALVAREZ, Michelle L.; 5200 Illumina Way, PCT/US2017/067836 San Diego, California 92122 (US). NIZIOLEK, Michael Adalbert; 5200 Illumina Way, San Diego, California 92122 (22) International Filing Date: (US). BRYAN, Debra Sue; 5200 Illumina Way, San Diego, 21 December 2017 (21.12.2017) California 92122 (US). DARLAND, Joshua Augustin; 5200 Illumina Way, San Diego, California 92122 (US). (25) Filing Language: English (26) Publication Language: English (74) Agent: SCHOLZ, Christian D. et al.; WEAVER AUSTIN VILLENEUVE & SAMPSON LLP, P.O. Box 70250, Oak- (30) Priority Data: land, California 94612-0250 (US). 62/442,765 05 January 2017 (05.01.2017) US (81) Designated States (unless otherwise indicated, for every 1704760.6 24 March 2017 (24.03.2017) GB kind of national protection available): AE, AG, AL, AM, 15/841,098 13 December 2017 (13.12.2017) US AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (71) Applicant: ILLUMINA, INC. [US/US]; 5200 Illumina CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, Way, San Diego, California 92122 (US). DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (72) Inventors: DREWS, Bradley Kent; 5200 Illumina Way, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, San Diego, California 92122 (US). WANG, Michael Dai; — MG, MK, MN, MW, MX, MY MZ, NA, NG, NI, NO, NZ, = 5200 Illumina Way, San Diego, California 92122 (US). UL- ego, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, MANELLA, Umberto; 5200 Illumina Way, San Diego, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, California 92122 (US). OSMUS, James Michael; 5200 Il-TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = lumina Way, San Diego, California 92122 (US). CLARK, = (54) Title: REAGENT NOZZLE SIPPER MIXING SYSTEM AND METHOD (57) : An analysis instrument may perform analytical operations on an analyte that is combined with multiple reagents prior to being introduced = 206 ASPIRATE AIR into a flow cell. The instrument may include a nozzle sipper that aspirates = SHIFT VALVE(S) FFE ASPIRATE BU R __, 208 204 reagents from a recipient, along with an analyte. The reagents may be directed = 210 ) ..- SHIFT VALVES) ) to a volume and may be repeatedly moved into and out of the volume by cy- = ASPIRATE R1 cling of a pump. The reagents may be ejected into a destination recipient with 212) 2 ( 214 ...= SHIFT YAMS) the nozzle sipper promoting vorticity in the recipient to enhance mixing. The = ASPIRATE RN repeated aspiration and ejection through the nozzle sipper effectively mixes = SHIFT VALVE(S) the reagents and the template in an automated or semi-automated fashion. = SHIFT VALVES) = _ ASPIRATE R1 - 90 V.= SHIFT VALVE(S) = ASPIRATE RN 1- 232 - = 218 .._, (234 NO ASPIRATE To MIX -YES 1238 11 EXPEL INTO OHL TO MIX DESTINATION RECIMENT -... 6 L -238 N 7r 240 NO D ONE GC GC NO 242 130 , EL INTO N ASPIRATE AIR DESTINATION RECIPIENT 11 ,-.... - FIG. 11 O N O [Continued on next page] WO 2018/128842 Al MIDEDIMOMOIDEIREEMOMOHHHEIEDIREM (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))