Patent SG-11201407794R-A (SG)
Title: Xylose fermenting yeast constructed using a modified genome shuffling method
Inventor: GENG ANLI
IPC: C12R1/865
Filed: 2013-05-27
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 28 November 2013 (28.11.2013) WIPOIPCT (10) International Publication Number WO 2013/176627 A1 (51) International Patent Classification: C12N15/08 (2006.01) C12P 7/10 (2006.01) C12N15/04 (2006.01) C12R 1/84 (2006.01) C12N1/19 (2006.01) C12R 1/865 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000214 27 May 2013 (27.05.2013) English (30) Priority Data: 201203879-0 25 May 2012 (25.05.2012) English SG (71) Applicant: NGEE ANN POLYTECHNIC [SG/SG]; 535 Clementi Road, Singapore 599489 (SG). (72) Inventors: GENG, Anli; c/o School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, 535 Clem­ enti Road, Singapore 599489 (SG). ZHANG, Wei; c/o School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, 535 Clementi Road, Singapore 599489 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. BOX 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (54) Title: XYLOSE FERMENTING YEAST CONSTRUCTED USING A MODIFIED GENOME SHUFFLING METHOD 100- 80 60 ® 2 40 o >> X 20- 0- 100 g/L xylose Xylose Ethanol Biomass —p— 20 —i— 40 —I— 60 50 40 30 ' O 20 i Ul 10 100 80 60 40 20 o <o Q o 80 100 l> CJ i> i-H cn i-H o CJ Time (h) Figure 3 (a) (57) Abstract: Disclosed is a method of providing a recombinant microorganism. The method comprises the steps of: (a) providing a hybrid microorganism comprising DNA from host a microorganism and donor a microorganism; and (b) fusing DNA extracted from a second microorganism into the hybrid microorganism to form the recombinant microorganism. Also disclosed are recombinant yeasts produced by the method of the present disclosure and a method of fermenting sugar using the recombinant yeast produced by the method of the present disclosure.