Patent SG-11201900231R-A (SG)
Title: Culture media for culturing pluripotent stem cells in suspension
Inventor: AMIT MICHAL
IPC: C12N5/0735
Filed: 2017-07-19
Abstract: 3 1o 1 10 4 10 5 10 1 11:1 2 Oct4-APC Fig. 23B Q1 Q2 92.3 3.55 03 1 59 Q2 0.15 Q3 0.74 111 11.11111 1 1111111 10 5 Q. 10 4 4 10 2 10 1 G1 1.99 10 4 10 2 ”ry O cc O (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 25 January 2018 (25.01.2018) W I PO I PCT Iiiimmomitia °nolo omm °loin loommo oimIE (10) International Publication Number WO 2018/015954 Al (51) International Patent Classification: C12N 5/0735 (2006.01) C12N 5/071 (2010.01) (21) International Application Number: PCT/IL2017/050812 (22) International Filing Date: 19 July 2017 (19.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/363,879 19 July 2016 (19.07.2016) US (71) Applicant: ACCELLTA LTD. [IL/IL]; Malat Building 3rd Floor, Campus of The Technion, 3200004 Haifa (IL). (72) Inventors: AMIT, Michal; 261 Yuvalim, 2014200 Mis- gav (IL). ANGEL, Itzchak; 22 Lotus Street, 7404578 Nes Ziona (IL). (74) Agent: EHRLICHE, Gal et al.; G. E. Ehrlich (1995) Ltd., 11 Menachem Begin Road, 5268104 Ramat Gan (IL). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (54) Title: CULTURE MEDIA FOR CULTURING PLURIPOTENT STEM CELLS IN SUSPENSION (57) : Provided are defmed culture media for culturing pluripotent stem cells in a suspension culture devoid of substrate adherence, the defmed culture media comprising an effective amount of a protease inhibitor; a GSK313 inhibitor and at least one agent selected from the group consisting of a protease inhibitor and a WNT3A polypeptide; a WNT3A polypeptide and a stabilizing agent thereof with the proviso that said stabilizing agent is not a lipid vesicle; and/or a GSK313 inhibitor, with the proviso that the medium is devoid of an ERK1/2 inhibitor. Also provided are cell cultures and methods of suing same. [Continued on next page] WO 2018/015954 Al MIDEDIMOM0101011EFIDINIROVINIMINHEMOVOIS TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))