Patent SG-11201907319Q-A (SG)
Title: Gamma delta t cells and a method of augmenting the tumoricidal activity of the same
Inventor: WANG SHU
IPC: C12N5/0783
Filed: 2018-02-08
Abstract: 70 60 50 a c6. Day 7 Figure 2A 10 - PBMC Day 17 40 30 20 O cc N 00 O O (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 16 August 2018 (16.08.2018) WIP0 1 PCT olimion °nolo DID mo IIV mollm IIII iflo oimIE (10) International Publication Number WO 2018/147805 Al (51) International Patent Classification: C12N 5/0783 (2010.01) A61P 35/00 (2006.01) A61K 35/17 (2015.01) (21) International Application Number: PCT/SG2018/050055 (22) International Filing Date: 08 February 2018 (08.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201701009Q 08 February 2017 (08.02.2017) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10, Connexis, Singapore 138632 (SG). (72) Inventors: WANG, Shu; c/o Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). ANG, Wei Xia; c/o Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). ZENG, Jieming; c/o Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). (74) Agent: YUSARN AUDREY; 24 Raffles Place #27-01 Clif- ford Centre, Singapore 048621 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: GAMMA DELTA T CELLS AND A METHOD OF AUGMENTING THE TUMORICIDAL ACTIVITY OF THE SAME 100 90 CTLA-4 (57) : The present invention relates to a method of generating T cells having at least one down-regulated co-inhibitory receptor, the method comprising the steps of: (a) culturing a population of cells comprising T cells with a phosphoantigen (i.e. zolendronic yS acid) to expand the yS T cells; and (b) culturing the expanded yS T cells with artificial antigen -presenting cells expressing a Fc receptor, and an anti-CD3 antibody. The present invention also relates to T cells generated according to a method of the present invention, yS preferably wherein the yS T cell expresses NKG2D chimeric antigen receptor, as well as methods of treatment and medical uses thereof, specifically for colorectal and ovarian cancers. [Continued on next page] WO 2018/147805 Al MIDEDIMOMMIDIREEMOMMEIDEEMOIREOMEMOIS Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(ii)) of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a))