Patent AU-2018200176-A1 (AU)
Title: Mutated protoporphyrinogen IX oxidase (PPX) genes
Inventor: GOCAL GREGORY F W
IPC: A01H5/00
Filed: 2018-01-09
Abstract: Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that 5 are herbicide resitant. Figure 1: Amino acid sequence of Arabidopsis thaliana chloroplast protoporphyrinogen oxidase (P POX - At4g01690) (Accession # AX084732) (SEQ ID NO: 1) 1 MELSLLRPTTQSLLPSFSKPNLRLNVYKPLRLRCSVAGGPTVGSSKIEGG 51 GGTTITTDCVIVGGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNII 5 101 TREENGFLWEEGPNSFQPSDPMLTMVVVDSGLKDDVLGDPTAPRFVLWNG 151 KLRPVPSKLTDLPFFDLMSIGGKIRAGFGALG:RPSPPGREESVEEFVRR 201 NLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWKLEQNGGSIIGGTF 251 KAIQERNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEAISARLGSKVKL 301 SWKLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPLS 10 351 ESAANALSKLYYPPVAASISYPKEAIRTECLIDGELKGFGQLHPRTQGV 401 ETLGTIYSSSLFPNRAPPGRTLLLNYIGGSTNTGILSKSEGELVEAVDRD 451 LRKMLIKPNSTDPLKLGVRVWPQAIPQFLVG2HFDILDTAKSSL'ISSGYEG 501 LFLGGNYVAGVALGRCVEGAYETAIEVNNFMSRYAYK 15 Figure 2: Nucleotide sequence of Arabidopsis thaliana chloroplast protoporphyrinogen oxidase (PPOX -At4gO 1690) (Accession # AX084732) (SEQ ID NO: 2) 1 TGACAAAATT CCGIAATTCTC TGCGATTTCC ATGGAGTTAT CTCTTCTCCG TCCGACGACT 61 CAATCGCTTC TTCCGTCGTT TTCGAAGCCC AATCTCCGAT TAAATGTTTA TAAGCCTCTT 20 121 AGACTCCGTT GTTCAGTGGC CGGTGGACCA ACCGTCGGAT CTTCAAAAAT CGAAGGCGGA 181 GGAGGCACCA CCATCACGAC GGATTGTGTG ATTGTCGGCG GAGGTATTAG TGGTCTTTGCr 241 ATCGCTCAGG CGCTTGCTAC TAAGCATCCT GATGCTGCTC CGAATTTAAT TGTGACCAG 301 GCTAAGGATC GTGTTGGAGG CAACATTATC ACTCGTGAAG AGAATGGTTT TCTCTGGGAA 361 GAAGGTCCCA ATAGTTTTCA ACCGTCTGAT CCTATGCTCA CTATGGTGGT AGATAGTGGT 25 421 TTGAAGGATG ATTTGGTGTT GGGAGATCCT ACTGCGCCAA GGTTTGTGTT GTGGAATGGG2 481 AAATTGAGGC CGGTTCCATC CAACCTAACA GACTTACCGT TCTTTGATTT GATGAGTATT 541 GGTGGGAAGA TTAGAGCTGG TTTTGGTGCA CTTGGCATTC GACCGTCACC TCCAGTCGT 601 GAAGAATCTG TGGAGGAGTT TGTACGGCGT AACCTCGGTG ATGAGGTTTT T'GAGCGCCTG 661 ATTGAACCGT TTTGTTCAGG TGTTTATGCT GCTGATCCTT CAAAACTGAG CATGAAAGCA 30 721 GCGTTTGGGA AGGTTTGGAA ACTAGAGCAA AATGGTGGAA GCATAATAGG TGGTACTTTT 781 AAGGCAATTC AGGAGAGGAA AAACGCTCCC AAGGCAGAAC GAGACCCGCG CCTGCCAAAA 841 CCACAGCGCC AAACAGTTGG TTCTTTCAGG AAGGGACTTC GAATGTTGCC AGAAGCAATA 901 TCTGCAAGAT TAGGTAGCAA AGTTAAGTTG TCTTGGAAGC TCTCAGGTAT CACTAAGCTG 961 GAGAGCGGAG GATACAACTT AACATATGAG ACTCCAGATG GTTTAGTTTC CGTGCAGAGC 35 1021 AAAAGTCTTG TAATGACGGT GCCATCTCAT GTTGCAAGTG GTCTCTTGCG CCCTCTTTCT 1081 GAATCTGCTG CAAATGCACT CTCAAAACTA TATTACCCAC CAGTTGCAGC AGTATCTATC 1141 TCGTACCCGA AAGAAGCAAT CCGAACAGAA TGTTTGATAG ATGGTGAACT AAAGGGTTTT 1201 CGGCAATTGC ATCCACGCAC GCAAGGAGTT GAAACATTAG GAACTATCTA CAGCTCCTCA 1261 CTCTTTCCAA ATCGCGCACC GCCCGGAAGA ATTTTGCTGT TGAACTACAT TGGCGGGTCT 40 1321 ACAAACACCG GAATTCTGTC CAAGTCTGAA GGTGAGTTAG TGGAAGCAGT TGACAGAGAT 1381 TTGAGGAAAA TGCTAATTAA GCCTAATTCG ACCGATCCAC TTAAATTAGG AGTTAGGGTA 1441 TGGCCTCAAG CCATTCCTCA GTTTCTAGTT GGTCACTTTG ATATCCTTGA CACGGCTAAA 1501 TCATCTCTAA CGTCTTCGGG CTACGAAGGG CTATTTTTGG GTGGCATTA CGTCGCTGGT 1561 GTAGCCTTAG GCCGGTGTGT AGAAGGCGCA TATGAAACCG CGATTGAGGT CAACAACTTC 45 1021 ATGTCACGGT ACGCTTACAA GTAAATGTAA AACATTAAAT CTCCCAGCTT GCGTGAQTTT 1681 TATTAAATAT TTTGAGATAT CCAAAAAAAA AAAAAAAAA Figure 3: Amino acid sequence of.Arabidopsis thaliana mitochondnal protoporphyrinogen oxidase (At5gl4220 Accession # NM 121426) (SEQ ID NO: 3) 50 1 MfASGAVADHQIEAVSGKRVAVVGAGVSGLAAAYKLKSRGLNVTVFEADGR 51 VGGKLRSVMQNGLIWDECANTMTEAEPEVGSLLDDLGLRKQQFPISQKK 1 01 RYIVRNGVPVMLPTNPIELVTSSVLSTQSKFQILLEPFLWKKKSSKVSDA 101 SAEESVSEFFQRH-iFGQEVVCYLIDPFVGGTSAADDSLSMKHSFPDLWW 101 EKSFGSIIVGAIRTKFAAKGGKSRDTKSSPGTKKGSRG8SFKGGMQILP 55 151 DTLCKSLSHDEINLDSKV8LSSYNSGSRQENWSLSCVSHNETQRQNPHYD 201 AV IMTAPLCNVJKEMt4KGGQFQLNFLPEINYMLSVLITTFTKEKIKR