Patent SG-11201811315P-A (SG)
Title: Reagent channel mixing system and method
Inventor: DREWS BRADLEY KENT
IPC: G01N35/02
Filed: 2017-12-21
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111111111111111111111111111111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\"\" WO 2018/128841 Al 12 July 2018 (12.07.2018) WIP0 1 PCT (51) International Patent Classification: (72) Inventors: DREWS, Bradley Kent; 5200 Illumina Way, G01N 35/10 (2006.01) G01N 35/04 (2006.01) San Diego, California 92122 (US). WANG, Michael Dai; BOIL 3/00 (2006.01) GO1N 35/00 (2006.01) 5200 Illumina Way, San Diego, California 92122 (US). UL- G01N 35/02 (2006.01) MANELLA, Umberto; 5200 Illumina Way, San Diego, California 92122 (US). CLARK, Stephen Wayne; 5200 (21) International Application Number: Illumina Way, San Diego, California 92122 (US). WHI- PCT/US2017/067834 TACRE, Johanna Lynn; 5200 Illumina Way, San Diego, (22) International Filing Date: California 92122 (US). PHELPS, Steven Scott; 5200 Illu- 21 December 2017 (21.12.2017) mina Way, San Diego, California 92122 (US). ALVAREZ, (25) Filing Language: English Michelle L.; 5200 Illumina Way, San Diego, California 92122 (US). NIZIOLEK, Michael Adalbert; 5200 Illumi- (26) Publication Language: English na Way, San Diego, California 92122 (US). BRYAN, De- bra Sue; 5200 Illumina Way, San Diego, California 92122 (30) Priority Data: (US). DARLAND, Joshua Augustin; 5200 Illumina Way, 62/442,772 05 January 2017 (05.01.2017) US San Diego, California 92122 (US). 1704758.0 24 March 2017 (24.03.2017) GB 15/841,102 13 December 2017 (13.12.2017) US (74) Agent: SCHOLZ, Christian D. et al.; WEAVER AUSTIN (71) Applicant: ILLUMINA, INC. [US/US]; VILLENEUVE & SAMPSON LLP, P. 0. BOX 70250, 5200 Illumina OAKLAND, California 94612-0250 (US). Way, San Diego, California 92122 (US). = (54) Title: REAGENT CHANNEL MIXING SYSTEM AND METHOD (-- 114 1fL 144 I PM \-.........38 1-1 Il 7r GC (57) : An analysis system GO introduced into a flow cell. The instrument ei one-by-one. The volume may be formed as a serpentine channel in a valve manifold associated with sippers for aspirating the reagents. Fe _ may perform operations on an analyte that may be combined with multiple regents prior to being may include a volume into which the reagents to be combined with the analyte are aspirated 1-1 - --- GO The reagents may then be mixed by cycling a pump to move the reagents within the mixing volume or channel. For this, the reagents , I may be aspirated from a recipient into the volume or channel, ejected back into the recipient, and this process may be performed 0 repeatedly to enhance mixing. ei C [Continued on next page] WO 2018/128841 Al IIMEDIMOMOIDEIREEMOMOHOHHEIVOIREM (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))