Patent SG-11201407652R-A (SG)
Title: Production cell line enhancers
Inventor: CHEN GANG
IPC: C12N15/79
Filed: 2013-05-29
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/181253 A1 (51) International Patent Classification: C07K16/00 (2006.01) C12N15/79 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/043116 29 May 2013 (29.05.2013) English English (30) Priority Data: 61/652,549 13/904,587 29 May 2012 (29.05.2012) US 29 May 2013 (29.05.2013) US (71) Applicant: REGENERON PHARMACEUTICALS, INC. [US/US]; 777 Old Saw Mill River Road, Tarrytown, New York 10591 (US). (72) Inventors: CHEN, Gang; 1600 Fox Tail Lane, Yorktown Heights, New York 10598 (US). BURAKOV, Darya; 10 Ashton Road, Yonkers, New York 10705 (US). DESH- PANDE, Dipali; 62 Eldorado Court, White Plains, New York 10603 (US). (74) Agent: JOHNSON, Mary C.; Regeneron Pharmaceutic­ als, Inc., Ill Old Saw Mill River Road, Tarrytown, New York 10591 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) — with sequence listing part of description (Rule 5.2(a)) CJ i-H 00 ^ (54) Title: PRODUCTION CELL LINE ENHANCERS 5 (57) Abstract: The present invention relates to discovery of the ectopic expression of EDEM2 in a production cell to improve the fS yield of useful multi-subunit a protein. Thus, the present invention provides for production cell lines, such as the canonical mam- malian biopharmaceutical production cell - the CHO cell, containing recombinant polynucleotides encoding EDEM2. Also disclosed (5T is a production cell containing both an EDEM2-encoding polynucleotide as well an XBP1 -encoding polynucleotide. Improved titers S of antibodies produced by these cell lines are disclosed, as well as the improved cell densities attained by these cells in culture.