Patent SG-11201805697W-A (SG)
Title: Multi-stage target cell enrichment using a microfluidic device
Inventor: BHAGAT ALI ASGAR S
IPC: B81B1/00
Filed: 2016-01-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111010111110111011110110111101111101101111101111011111 International Bureau 0.. .... .. (10) International Publication Number ..... ..or::,,, (43) International Publication Date WO 2017/131580 Al 3 August 2017 (03.08.2017) WI P0 I P CT (51) International Patent Classification: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, B81B 1/00 (2006.01) B07C 5/12 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, BOIL 3/00 (2006.01) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, (21) International Application Number: MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PCT/SG2016/050040 PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (22) International Filing Date: SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 28 January 2016 (28.01.2016) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (8 4 ) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicant: CLEARBRIDGE BIOMEDICS PTE LTD TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, [SG/SG]; 81 Science Park Drive, #02-03 Singapore Sci- TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, ence Park 1, Singapore 118257 (SG). DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (72) Inventors: BHAGAT, Ali Asgar S.; 63 Hume Avenue, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, #10-05, Singapore 598742 (SG). GUAN, Guofeng; 1 Nor- GW, KM, ML, MR, NE, SN, TD, TG). manton Park, #13-153, Singapore 118998 (SG). Declarations under Rule 4.17: (74) Agent: PATEL, Upasana; Marks & Clerk Singapore LLP, — of inventorship (Rule 4.17(N)) Tanjong Pagar, P 0 Box 636, Singapore 910816 (SG). — (81) Designated States (unless otherwise indicated, for every Published: _= kind of national protection available): AE, AG, AL, AM, — with international search report (Art. 21(3)) — AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, = = Title: MULTI-STAGE TARGET CELL ENRICHMENT USING A MICROFLUIDIC DEVICE (54) = 200 212 s 25 , .. 254 B 2 2nd stage wo2,,, = — _ 220 2 6 ._ 214 , D 272 270 S o 111 268 = = 210 44 ! 44%, 25. il ly 266 -If 260 250 = = = 218 = ' A. 64 . 222 — 23 d ., 262 224 4 / \ .... 1s t stage 226 Woi \-- _ 234 1-1 232 Figure 2a .4 240 of (57) : A microfluidic device comprises at least one inlet for receiving a sample comprising target cells and non-target cells; kr) a first spiral channel portion having an upstream end in a central region and a downstream end in a peripheral region, the upstream - end being coupled to the inlet, the first spiral channel portion being configured such that the target cells and the non- target cells oc - f.r) cupy different streams at the downstream end; a first waste outlet arranged to couple with streams of non-target cells at the down - I-1 ---- stream end of the first spiral channel portion; a link channel portion arranged to couple with streams of target cells at the down - I N stream end of the first spiral channel portion; a second spiral channel portion having an upstream end in a peripheral region and a 1-1 C downstream end in a central region, the upstream end of the second channel portion being coupled to the link channel portion, the ei second spiral channel portion being configured such that the target cells and the non-target cells occupy different streams at the 0 downstream end; a second waste outlet arranged to couple with streams of non-target cells at the downstream end of the second spir - ,-t al channel portion; and a sample outlet arranged to couple with streams of target cells at the downstream end of the second spiral channel portion.