Patent SG-11201808869W-A (SG)
Title: Affinity chromatography devices
Inventor: MCMANAWAY MICHAEL
IPC: G01N30/60
Filed: 2017-03-29
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11111111011110111010101111101011111011101011111101111010111011111101111011111 International Bureau 0.. .1 j ..... ..or::,„, (10) International Publication Number (43) International Publication Date WO 2017/176522 Al 12 October 2017(12.10.2017) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every BOlD 15/22 (2006.01) GO1N 30/60 (2006.01) kind of national protection available): AE, AG, AL, AM, BOlD 15/38 (2006.01) B01J 20/28 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BOlD 63/08 (2006.01) B01J 20/286 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, BOlD 63/10 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (21) International Application Number: KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, PCT/US2017/024686 MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (22) International Filing Date: NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, 29 March 2017 (29.03.2017) RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (25) Filing Language: English ZA, ZM, ZW. (26) Publication Language: English (84) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of regional protection available): ARIPO (BW, GH, 15/094,428 8 April 2016 (08.04.2016) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 15/409,733 19 January 2017 (19.01.2017) US TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (71) Applicant: W.L. GORE & ASSOCIATES, INC. DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, [US/US]; 555 Paper Mill Road, Newark, DE 19711 (US). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, (72) Inventors: MCMANAWAY, Michael, C.; 245 N Bo- hernia Avenue, Cecilton, MD 21913 (US). SWETLIN, GW, KM, ML, MR, NE, SN, TD, TG). Brian, J.; 300 Hannum Mill Road, West Grove, PA 19390 Published: (US). ZUKOR, Kenneth, S.; 229 Canvas Back Drive, Havre De Grace, MD 21078 (US). with international search report (Art. 21(3)) — before the expiration of the time limit for amending the (74) Agents: JOHNS, David, J. et al.; W.L. Gore & Associates, claims and to be republished in the event of receipt of Inc., 551 Paper Mill Road, P.O. Box 9206, Newark, DE amendments (Rule 48.2(h)) = 19714-9206 (US). = (54) Title: AFFINITY CHROMATOGRAPHY DEVICES 7 150 25 L Mr 25 2Q 11 N 110 25 25 4R N as ir) FIG. 7 ,tD I N (57) : The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from Il ---- an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane as- IN sembly or a wound membrane assembly. The membrane assemblies' include (1) at least one polymer membrane that contains therein IN C inorganic particles and (2) at least one impermeable layer (e.g., a thermoplastic polymer in a solid state). The polymer membrane N and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysac - 0 charide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be ,..1: cleaned with a caustic solution between uses. The chromatography devices has a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.