Patent SG-11201909765X-A (SG)
Title: An improved method for high level production of crm
Inventor: MASILAMANI BALAMURALI
IPC: C07K14/34
Filed: 2018-04-19
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 25 October 2018 (25.10.2018) WIP0 I PCT omit VIII °nolo om mil olo Ho Immo (10) International Publication Number WO 2018/193475 A2 (51) International Patent Classification: Not classified (21) International Application Number: PCT/IN2018/050235 (22) International Filing Date: 19 April 2018 (19.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 201741014335 22 April 2017 (22.04.2017) IN (71) Applicant: BIOLOGICAL E LIMITED [IN/IN]; 18/1 & 3, Azamabad, Hyderabad, Telangana, HYDERABAD 500020 (IN). (72) Inventors: MASILAMANI, Balamurali; Biological E Limited, 18/1 & 3, Azamabad, Hyderabad, Telangana, HY- DERABAD 500020 (IN). SRIRAMAN, Rajan; Biological E Limited, 18/1 & 3, Azamabad, Hyderabad, Telangana, HYDERABAD 500020 (IN). DIXIT, Mandar Shirish; Biological E Limited, 18/1 & 3, Azamabad, Hyderabad, Telangana, HYDERABAD 500020 (IN). CHAKKA, De- viprasanna; Biological E Limited, 18/1 & 3, Azamabad, Hyderabad, Telangana, HYDERABAD 500020 (IN). NAI- DU, Sureddisatyam; Biological E Limited, 18/1 & 3, Azamabad, Hyderabad, Telangana, HYDERABAD 500020 (IN). MATUR, Ramesh Venkat; Biological E Limited, 18/1 & 3, Azamabad, Hyderabad, Telangana, HYDER- ABAD 500020 (IN). MANTENA, Narender Dev; Biologi- cal E Limited, 18/1 & 3, Azamabad, Hyderabad, Telangana, HYDERABAD 500020 (IN). DATLA, Mahima; Biologi- cal E Limited, 18/1 & 3, Azamabad, Hyderabad, Telangana, HYDERABAD 500020 (IN). (74) Agent: MANDHANE, Ramachandra R.; Kayser & Com- pany, 2nd Floor Raja Bahadur Mansion, 20, Ambalal Doshi Marg, Fort, Mumbai, Maharashtra, MUMBAI 400001 (IN). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (54) Title: AN IMPROVED METHOD FOR HIGH LEVEL PRODUCTION OF CRM Overlap extend PCR 1XX Amp/median& cif:7197 diphtherteeATCC 53821 cassette from C. Amplification of eri/t cassette FIGURE 1 (57) : The present invention provides an improved method for the production of CRM197 with high yield using engineered 1-1 Corynebacterium diphtheria strain having increased copy number of CRM197 gene, wherein the method comprises growing the strain ei in a media free of animal derived components with one or more amino acids. O [Continued on next page] WO 2018/193475 A2 lilillEHEIMIRENOMOMBEEMEOlinii# SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: of inventorship (Rule 4.17 (iv)) Published: without international search report and to be republished upon receipt of that report (Rule 48.2(g)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE