Patent SG-11201808914U-A (SG)
Title: Methods, systems and kits for in-pen assays
Inventor: LIONBERGER TROY A
IPC: G01N21/75
Filed: 2017-04-14
Abstract: 19) (43) 19 INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) World Intellectual Property -, Organization 1111111101111011101010111110101111101110111111110111011110111101111101111011111 International Bureau .. .... ..Yejd .... ...,,, (10) International Publication Number International Publication Date WO 2017/181135 A2 October 2017(19.10.2017) WIPO I PCT (51) (21) (22) (25) Filing Language: (26) Publication Language: (30) (71) (72) Inventors: LIONBERGER, Troy A.; 5858 Horton Street, _ International Patent Classification: Tanner; 5858 Horton Street, Suite 320, Emeryville, Cali- GO1N 21/64 (2006.01) B81B 7/02 (2006.01) fornia 94608 (US). WANG, Gang F.; 5858 Horton Street, GO1N 21/75 (2006.01) Suite 320, Emeryville, California 94608 (US). LAVIEU, Gregory G.; 5858 Horton Street, Suite 320, Emeryville, International Application Number: PCT/US2017/027795 California 94608 (US). TENNEY, John A.; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). International Filing Date: KARUNAKARAN, Aathavan; 5858 Horton Street, Suite 14 April 2017 (14.04.2017) 320, Emeryville, California 94608 (US). SINGHAL, Anupam; 5858 Horton Street, Suite 320, Emeryville, Cali- English fornia 94608 (US). LIN, I-Jong; 5858 Horton Street, Suite English 320, Emeryville, California 94608 (US). Priority Data: (74) Agents: KUAN, Roger C. et al.; K&L Gates, 630 Hansen 62/323,500 15 April 2016 (15.04.2016) US Way, Palo Alto, California 94304 (US). 62/364,568 20 July 2016 (20.07.2016) US (81) Designated States (unless otherwise indicated, for every Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 kind of national protection available): AE, AG, AL, AM, Horton Street, Suite 320, Emeryville, California 94608 AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (US). BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, Suite 320, Emeryville, California 94608 (US). FOWLER, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, Matthew E.; 5858 Horton Street, Suite 320, Emeryville, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, California 94608 (US). ELMS, Phillip J. M.; 5858 Horton NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, Street, Suite 320, Emeryville, California 94608 (US). RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, LOUTHERBACK, Kevin D.; 5858 Horton Street, Suite TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, 320, Emeryville, California 94608 (US). LOWE, JR, Ran- ZA, ZM, ZW. dall D.; 5858 Horton Street, Suite 320, Emeryville, Cali- fornia 94608 (US). GONG, Jian; 5858 Horton Street, (84) Designated States (unless otherwise indicated, for every Suite 320, Emeryville, California 94608 (US). NEVILL, J. kind of regional protection available): ARIPO (BW, GH, [Continued on next page] — — = = and METHODS, SYSTEMS AND KITS FOR IN-PEN ASSAYS the methods, sys - and an analyte of of a biological mi - = (54) Title: = 242 122 428 406 the results of an assay. diffusion rates of a reporter analyte may be a secreted product = = : in order to quantify an amount of analyte in a microfluidic device. The 424 426 $ * 402 404 FIG. 4C Methods, systems and kits are described herein for detecting devices of the present disclosure rely on a difference between the N = = = = _ = _ _ a =413 X =412 E-kX 414 kin en 11 11 GC ,-, IN ,-, (57) 0 tems \" interest Q cro-object. In particular, molecule WO 2017/181135 A2 111114113101ThigThil1130111B30111MMOIS GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, — as to the applicant's entitlement to claim the priority of TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, the earlier application (Rule 4.17(iii)) DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, Published: LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, — without international search report and to be republished SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). upon receipt of that report (Rule 48.2(g)) — Declarations under Rule 4.17: with sequence listing part of description (Rule 5.2(a)) — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(ii))