Patent SG-11201900669W-A (SG)
Title: Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
Inventor: BORRA-GARSKE MARGIE
IPC: C12N15/53
Filed: 2017-08-08
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 March 2018 (01.03.2018) o I iolo 011111 OM REHM IMO 011 (10) International Publication Number WO 2018/038906 Al WIPO I PCT (51) International Patent Classification: CO7H 21/00 (2006.01) C12N 9/06 (2006.01) C07K 14/435 (2006.01) Cl 2N 15/52 (2006.01) C12N 9/00 (2006.01) C12N 15/53 (2006.01) (21) International Application Number: PCT/US2017/045838 (22) International Filing Date: 08 August 2017 (08.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/380,165 26 August 2016 (26.08.2016) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). (72) Inventors: BORRA-GARSKE, Margie, Tabuga; 200 Penobscot Drive, Redwood City, California 94063 (US). ALVIZO, Oscar; 200 Penobscot Drive, Redwood City, California 94063 (US). MAYO, Melissa, Ann; 200 Penob- scot Drive, Redwood City, California 94063 (US). JENNE, Stephan; 200 Penobscot Drive, Redwood City, California 94063 (US). SOWELL-KANTZ, Auric, Anthony; 200 Penobscot Drive, Redwood City, California 94063 (US). MOLINARO, Carmela; 126 East Lincoln Avenue, Rah- way, New Jersey 07065 (US). TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 1-1 TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, O GC Cr) O (54) Title: ENGINEERED MINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS 0 (57) : The present application provides engineered polypeptides having imine or oxime reductase activity, polynucleotides N encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engi- C neered polypeptides with a range of ketone amine substrate compounds to prepare secondary and tertiary amine product compounds.