Patent SG-11201810633W-A (SG)
Title: New fluorescent dyes and their uses as biomarkers
Inventor: ROMANOV NIKOLAI
IPC: C07D491/04
Filed: 2017-09-29
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1 Ill 11111 0 11101 HOE HI 1 0 0111011001111111101011 MEV OEN Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\"1 WO 2018/060482 Al 05 April 2018 (05.04.2018) WIP0 I PCT (51) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) (71) Applicant: (72) Inventor: ROMANOV, Nikolai; International Patent Classification: (81) Designated States (unless otherwise indicated, for every C07D 417/04 (2006.01) A61K 49/10 (2006.01) kind of national protection available): AE, AG, AL, AM, CO7D 491/04 (2006.01) CO9K 11/06 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, PCT/EP2017/074880 DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 29 September 2017 (29.09.2017) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, English TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Priority Data: (84) Designated States (unless otherwise indicated, for every 62/402,635 30 September 2016 (30.09.2016) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, ILLUMINA CAMBRIDGE LIMITED UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, [GB/GB]; Chesterford Research Park, Little Chesterford, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Nr. Saffron Walden Essex CB10 1XL (GB). EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, c/o Illumina Cambridge MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, Limited, Chesterford Research Park, Little Chesterford, Nr. TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Saffron Walden Essex CB10 1XL (GB). KM, ML, MR, NE, SN, TD, TG). Agent: MURGITROYD & COMPANY; Scotland House, Declarations under Rule 4.17: 165-169 Scotland Street, Glasgow Strathclyde G5 8PL _ as to applicant's entitlement to apply for and be granted a (GB). patent (Rule 4.1700) (74) = = = = D O V , CD °, Z n 0 1 —i 0 0 a c. , 0 .., m0 C/ ) n r r l Fluorescen Inte t ns ity ( Ar bitrary Un it) g Z n = H co 2, R ( o DYES AND THEIR USES AS BIOMARKERS / R ) R N g i R3 0 0 N I R2 R 4 (I) labels. The compounds may be (54) = = = = = = 1 © © -.... 1-1 N (57) _ used 250 200 150 100 50 0 -• r • 0 \ \ . . ill 11. \ \\ / 4 1 \ .\\ i / 0 \\ \ \\ / \ . • 6 1 4 \\ \.\\ \ \ \‘• \ \ • \ s \• \\\,'• — ffc-star440sx — — ffc-1-9 -- ffc-I-10 --- ffc-1-6 ---- ffc-1-1 470 520 570 620 670 720 770 Wavelength (nm) \" 171c 1 relates to new fluorescent dyes and their uses as fluorescent in nucleic acid sequencing applications. [Continued on next page] WO 2018/060482 Al 11111111111111011101010111110111010111110110011111111111101011111110110111111 Published: — with international search report (Art. 21(3))