Patent EA-002980-B1 (EA)
Title: Chimeric construct comprising at least two genes capable of conferring tolerance of a plant to herbicides, a plant cell comprising said construct, a plant capable of conferring tolerance to at least herbicides, and method of treating plants by herbicides
Inventor: PALETT KEN
IPC: C12R1/38
Filed: 1997-07-10
Abstract: 1. A chimeric construct comprising at least two basic chimeric genes wherein each of said basic chimeric genes comprises regulatory elements necessary for transcription in a plant operably connected to a coding sequence encoding an enzyme that is capable of conferring tolerance to a herbicide on a plant, characterized in that at least one of said coding sequences encodes hydroxyphenyl pyruvate dioxygenase (HPPD). 2. The chimeric construct according to claim 1, characterized in that it further comprises a third basic chimeric gene comprising a coding sequence that encodes an enzyme that is capable of conferring herbicide tolerance on a plant. 3. The chimeric construct according to claim 1 or 2, characterized in that at least one coding sequence is derived from a Klebsiella. ap. nitrilase gene which is capable of conferring tolerance to a herbicide of the dihalohydroxybenzonitrile family on a plant. 4. The chimeric construct according to claim 3, characterized in that the herbicide is bromoxynil. 5. The chimeric construct according to claim 3, characterized in that the herbicide is ioxynil. 6. The chimeric construct according to claim 1 or 2, characterized in that at least one coding sequence encodes an enzyme which is capable of conferring tolerance to glyphosate on a plant. 7. The chimeric construct according to claim 1 or 2, characterized in that at least one coding sequence encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme which is capable of conferring tolerance on a plant to a herbicide that inhibits EPSPS in said plant. 8. The chimeric construct according to claim 7, characterized in that the second coding sequence encoding EPSPS enzyme is capable of conferring tolerance to glyphosate on a plant. 9. The chimeric construct according to claim 6, characterized in that the second coding sequence encodes glyphosate oxidoreductase, an enzyme of glyphosate detoxification. 10. The chimeric construct according to any one of claims 1 to 9, characterized in that the sequence encoding HPPD is derived from Pseudomonas sp. 11. The chimeric construct according to claim 10, wherein the Pseudomonas sp is Pseudomonas fluorescens. 12. The chimeric construct according to any one of claims 1 to 9, characterized in that the sequence encoding HPPD is derived from a plant. 13. The chimeric construct according to claim 12, wherein the plant is Arabidopsis thaliana. 14. Chimeric construct according to claim 12, wherein the plant is Daucus carota. 15. A vector comprising the chimeric construct according to any one of claims 1 to 14. 16. The vector according to claim 15, characterized in that it consists of a plasmid. 17. A plant cell, characterized in that it contains at least one chimeric gene according to any one of claims 1 to 14. 18. A plant regenerated from the transformed cell, characterized in that it is regenerated from the cell comprising the plant cell according to claim 17. 19. A method for producing transformed plants capable of expressing tolerance to at least two herbicides, characterized in that comprising introducing the chimeric construct according to any one of claims 1 to 14 and that the transformed cells are subjected to regeneration. 20. The method of treating plants by herbicides according to claim 18. 21. The method of treating a plant comprising applying at least two herbicides according to claim 20. 22. The method of treating a plant comprising applying at least three herbicides according to claim 20. 23. The method according to claim 20 or 22, characterized in that one of the herbicides is an HPPD inhibitor. 24. The method according to any one of claims 21 to 23, characterized in that the herbicides are applied simultaneously. 25. The method according to claim 27, characterized in that the herbicides are applied in the form of a single composition. 26. The method according to claim 25, characterized in that the herbicides composition is in the form of a mixture which is freshly prepared before application. 27. The method according to any one of claims 20 to 23, characterized in that the herbicides are applied in succession. 28. The method according to any one of claims 20 to 27, characterized in that the HPPD inhibitor is isoxaflutole. 29. The method according to any one of claims 20 to 27, characterized in that the HPPD inhibitor is sulcotrione. 30. The method according to any one of claims 20 to 29, characterized in that one of the herbicides belongs to the dihydroohydroxybenzonitrile family. 31. The method according to claim 30, characterized in that the herbicide is selected from the group consisting of bromoxynil and ioxynil. 32. The method according to any one of claims 20 to 31, characterized in that the second herbicide is an EPSPS inhibitor. 33. The method according to claim 32, wherein the EPSPS inhibitor is glyphosate or sulfosate.