Patent BG-113328-A (BG)
Title: Technology for preparation and composition of biologically active product containing a probiotic complex, a prebiotic, pectins, a vitamin complex and extracts
Inventor: KIRCHEV DIMITAR
IPC: C12N1/20
Filed: 2021-02-23
Abstract: The technology is implemented by sequentially proceeding through the following phases: 1. Initial creation of a matrix structure; 2. Integration of the probiotic complex into the matrix structure; 3. Inclusion of the active components - vitamin complex - vitamins C, K2, D3, as well as the trace elements - zinc and selenium; 4. Inclusion of the extracts - echinacea, turmeric and ginger. The phases of the technology in content and technological aspects proceed as follows: 1. Formation of matrix structure by processing the modified ingredients in a hermetically designed chamber mixer, the formation of matrix structure is realized at pre-set values of the following technological parameters: temperature - 42°C, vacuum 0.5 MPa, pH 4.0, rotation rate - 32° rpm, complex penetration level - 12.5. As a result of these processes, a matrix-structured vector-oriented polymolecular structure is created. 2. Integration of the probiotic complex into the matrix structure. It is carried out in a specialized module observing the following parameters of the technological operation: temperature - 8°C, pH - 5.5, vacuum 1.5 MPa, ultrasonic wave frequency 12 Hz. This ensures precise incorporation of the probiotic complex (Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus salivarius ssp. Thermophilus, Lactobacillus casei subsp. Casei, Lactobacillus reuteri) or (Lactobacillus bulgaricus, Bifidobacterium bifidum, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium adolescentis) and the prebiotic - inulin in the matrix structure, obtaining a polymolecular biocomplex with controlled activation of internal bonding interactions in the resulting mixture. All of this results in obtaining a multicultural combination between the probiotic complex and the polymolecular matrix structure, and hence increased activity and vitality of the probiotic strains. 3. Incorporation of the active components - vitamin complex - vitamins C, K2, D3, as well as the trace elements - zinc and selenium. This is carried out by layer fragmented phase injection into the surface layer of the matrix complex created before this operation.   It is realized under strict observance of the values of the parameters characterizing the technological process - temperature - 35°C, pressure - 1.5 atm, degree of atomization and application on the surface of the matrix complex 12 g/h, vacuum 0.5 MPa. 4. The last phase is the incorporation of the extracts of echinacea, turmeric and ginger into the constructed combined matrix complex. The mixture of extracts is incorporated into the hitherto structured complex by nanofragmentor layered dry injection through controlled rotational application of the extracts, achieving a high degree of homogeneity. The controlled parameters during the execution of this technological phase are the following values - speed of the fragmentation cell integrated in the technological module - 750 rpm/h, pressure - 1.5 atm, temperature - 35°C, degree of swirl and rotation - 85 rpm/45°.