Patent SG-11201901991Q-A (SG)
Title: Methods of detecting per cell pd-l1 expression and uses thereof
Inventor: PATTERSON BRUCE K
IPC: G01N33/574
Filed: 2017-09-06
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#11101111 0 11101 HOE 3E1 0 011101111110111111 OHRE III OEN Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2018/048936 Al 15 March 2018 (15.03.2018) WIP0 I PCT — (51) (21) (22) (25) Filing Language: (26) (30) (71) (72) (71) International Patent Classification: (74) Agent: FIELD, Bret E.; 201 Redwood Shores Parkway, G01N 1/30 (2006.01) G01N 33/574 (2006.01) Suite 200, Redwood City, California 94065 (US). G01N 33/483 (2006.01) G01N 15/14 (2006.01) (81) Designated States (unless otherwise indicated, for every International Application Number: kind of national protection available): AE, AG, AL, AM, PCT/US2017/050322 AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, International Filing Date: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, 06 September 2017 (06.09.2017) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, English KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, Publication Language: English MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, Priority Data: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, 62/384,037 06 September 2016 (06.09.2016) US TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Applicant: INCELLDX, INC. [US/US]; 1700 El Camino (84) Designated States (unless otherwise indicated, for every Real, Menlo Park, California 94027 (US). kind of regional protection available): ARIPO (BW, GH, Inventors; and GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, Applicants: PATTERSON, Bruce K. [US/US]; 274 TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Mosher Way, Palo Alto, California 94304 (US). EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, CHARGIN, Amanda Noel [US/US]; 11 Jackson Street, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, San Jose, California 95112 (US). SHULTS, Keith TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, [US/US]; 9825 Sam Donald Road, Nolensville, Tennessee KM, ML, MR, NE, SN, TD, TG). 37135 (US). = = = Title: METHODS OF , — ..-_-, (54) DETECTING PER CELL PD-L1 EXPRESSION AND USES THEREOF , of neoplasia cells. to detect whether addition, kits that find = = C , pos(77 20 %) PD-L1 pos(0.21 %) = = = = _ = = : Methods are of the methods include cytometrically assaying a labeled cell suspension neoplastic cell that expresses PD-L1 above a predetermined threshold in practicing the subject methods are also provided. -- -- GO Aspects 1 i 0 2 10' 10• 10 5 10 7 10' APC-A FIG, 1 provided for detecting the per cell programmed 1 . Q - 10 4 10 5 1 0 5 10 7 APC-A -death ligand 1 (PD-L1) expression to quantify per cell PD-L 1 expression is present in the neoplasia sample. In _ Il .4 M tz. co ii- © (57) ,-1 a 0 use ei O [Continued on next page] WO 2018/048936 Al MIDEDIMOMMIDEFIEHVIVEHEMERMOVOIS Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event amendments (Rule 48.2(h)) of receipt of