Patent DE-102005062984-A1 (DE)
Title: Washing or cleaning composition containing specific amylase variants, useful for dishwashing and laundry compositions, have increased stability especially against oxidizing agents
Inventor: BESSLER CORNELIUS
IPC: C11D3/386
Filed: 2005-12-28
Abstract: Washing and cleaning composition (A) contains an alpha -amylase (aA) variant (I) that is derived from (a) a starting aA homologizable with the aA designated AA560 (sequence reproduced) by amino acid chnages at positions 9, 149, 182, 186, 202, 257, 295, 299, 323 or 345, optionally also at other positions or (b) from AA560 by introducing any of 90 specified combinations of mutations. Washing and cleaning composition (A) contains an alpha -amylase (aA) variant (I) that is derived from (a) a starting aA homologizable with the aA designated AA560 (sequence reproduced) by amino acid chnages at positions 9, 149, 182, 186, 202, 257, 295, 299, 323 or 345, optionally also at other positions or (b) from AA560 by introducing any of 90 specified combinations of mutations. These mutations are (one-letter amino acid abbreviations) are: (1)M9L/M202I; (2) M9L/M202I/M323T; (3) M9L/M202I/M323T/M382Y; (4)M9L/M202I/Y295F/A339S; (5)M9L/M202I/Y295F; (6)M9L/M202I/A339S; (7)M9L/M202IY295F/A339S; (8)M9L/M202IY295F/A339S/E345R; (9)M9L/G149A/M202I/Y295F/A339S/E345R; (10)-(18) as (1)-(9) but M202L rather than M202I; (19)-(27) as (1)-(9) but M202T rather than M202I; (28) M9L/G149A/M202I/V214T/Y295F/N299Y/M323T/A339S/E345R; (29) M9L/G149A/M202L/V214I/Y295F/M323T/A339S/E345R/M382Y; (30) M9L/G149A/G182T/G186AM202I/V214I/Y295F/N299Y/M323T/A339S; (31) M9L/G149A/G182T/G186A/M202L/T257I/Y295F/N299Y/M323T/A339S/E345R; (32) M9L/G149A/M202L/V214T/Y295F/N299Y/M323T/A339S/E345R; (33) M9L/G149A/M202I/V214I/Y295F/M323T/A339S/E345R; (34) M9L/G149A/G182T/G186A/M202L/V214I/Y295F/N299Y/M323T/A339S; (35) M9L/G149A/G182T/G186A/M202I/T257I/Y295F/N299Y/M323T/A339S/E345R; (36) M9L/G149A/M202I/V214T/Y295F/N299Y/M323T/A339S/E345R/N471E; (37) M9L/G149A/M202L/V214I/Y295F/M323T/A339S/E345R/M382Y/N471E; (38) M9L/G149A/G182T/G186A/M202I/V214I/Y295F/N299Y/M323T/A339S/N471E; (39) M9L/G149A/G182T/G186A/M202L/T257I/Y295F/N299Y/M323T/A339S/E345R/N471E; (40) M202L/M105F/M208F; (41) G133E/M202L/Q361E; (42) G133E/M202L/R444E; (43) M202L/Y295F; (44) M202L/A339S; (45) M202L/M323T; (46) M202L/M323T/M309L; (47) M202L/M323T/M430I; (48) M202L/V214T/R444Y; (49) M202L/N283D/Q361E; (50) M202L/M382Y/K383R; (51)M202L/K446R/N484Q; (52) M202I/Y295F; (53) M202I/A339S; (54) M202I/M105F/M208F; (55) G133E/M202I/Q361E; (56) G133E/M202I/R444E; (57) M202I/M323T; (58) M202I/M323T/M309L; (59) M202I/M323T/M430I; (60) M202I/V214T/R444Y; (61) M202I/N283D/Q361E; (62) M202I/K446R/N484Q; (63) M202I/K446R/N484Q; (64) M202V/M105F/M208F; (65) G133E/M202V/Q361E; (66) G133E/M202V/R444E; (67) M202V/M323T; (68) M202V/M323T/M309L; (69) M202V/M323T/M430I; (70) M202V/M323T/M9L; (71) M202V/V214T/R444Y; (72) M202V/N283D/Q361E; (73) M202V/M382Y/K383R; (74) M202V/K446R/N484Q; (75) M202T/M105F/M208F; (76) G133E/M202T/Q361E; (77) G133E/M202T/R444E; (78) M202T/Y295F; (79) M202T/A339S; (80) M202T/M323T; (81) M202T/M323T/M309L; (82) M202T/M323T/M430I; (83) M202T/M323T/M9L; (84) M202T/V214T/R444Y; (85) M202T/N283D/Q316E; (86) M202T/A339S; (87) M202T/Y925F; (88) M202T/N299F,Y; (89) M202T/M382Y/K383R; or (90) M202T/K446R/N484Q. Independent claims are included for the following: (1) combination product comprising, in two separate packs, agent A comprising 10-75wt.% builder; 0.1-10wt.% (I) and the balance water; and agent B comprising 10-75wt.% builder and the balance water; and (2) similar combination product in which agent B contains 0.1-15wt.% bleaching agent.