Patent SG-11201908489X-A (SG)
Title: De novo synthesized combinatorial nucleic acid libraries
Inventor: COX ANTHONY
IPC: C12N5/071
Filed: 2018-03-14
Abstract: VariantsExpected vs Observed Frequenci. Expected Probability —Observed — — .00 5.00E-06 8.00 4.50E-06 7.00 4.00E-06 4?: 3.50E-06 6.00 3.00E-06 (5 5.00 2.50E-06 EL 4.00 2.00E-06 e-I 3.00 1.50E-06 2.00 1.00E-06 1.00 5.00E-07 0.00 0.00E+00 Ave rag e Coun t (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 20 September 2018 (20.09.2018) WIPO I PCT IiiimmommionotiolomolomollumioommovoimIE (10) International Publication Number WO 2018/170164 Al (51) International Patent Classification: C40B 30/06 (2006.01) C12N 5/071 (2010.01) C40B 40/08 (2006.01) C12N 5/10 (2006.01) C4OB 50/02 (2006.01) C12Q 1/68 (2018.01) C4OB 50/06 (2006.01) A61K 31/711 (2006.01) C12N 15/66 (2006.01) (21) International Application Number: PCT/US2018/022487 (22) International Filing Date: 14 March 2018 (14.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/471,723 15 March 2017 (15.03.2017) US 62/578,326 27 October 2017 (27.10.2017) US (71) Applicant: TWIST BIOSCIENCE CORPORATION [US/US]; 455 Mission Bay Blvd. South, Suite 545, San Francisco, California 94158 (US). (72) Inventors: COX, Anthony; 455 Mission Bay Blvd. South, Suite 545, San Francisco, California 94158 (US). CHEN, Siyuan; 455 Mission Bay Blvd. South, Suite 545, San Fran- cisco, California 94158 (US). LEDOGAR, Charles; 455 Mission Bay Blvd. South, Suite 545, San Francisco, Califor- nia 94158 (US). TOPPANI, Dominique; 455 Mission Bay Blvd. South, Suite 545, San Francisco, California 94158 (US). (74) Agent: HARBURGER, David; WILSON SONSINI GOODRICH & ROSATI, 650 Page Mill Road, Palo Alto, California 94304 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (54) Title: DE NOVO SYNTHESIZED COMBINATORIAL NUCLEIC ACID LIBRARIES FIG. 22 (57) : Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease. [Continued on next page] WO 2018/170164 Al MIDEDIMOMOIDEIREEMOMMEIROHEOHMEn HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(H)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))