Patent EA-003204-B1 (EA)
Title: A kit for carrying out retrovirus-mediated gene transfer into target cells
Inventor: ASADA KIYOZO
IPC: A61K48/00
Filed: 1996-11-07
Abstract: 1.A kit for carrying out retrovirus-mediated gene transfer into target cells, which comprises: (a) an effective amount of a functional material having retrovirus binding domain and/or an effective amount of another functional material having target cell binding domain; (b) an artificial substrate for incubating the retrovirus and the target cells; and (c) a target cell growth factor for pre-stimulating the target cells. 2. A kit according to claim 1, wherein the functional material is selected from Heparin-II binding region of fibronectin, fibroblast growth factors, collagens, polylysines and functional equivalents thereof. 3. A kit according to claim 1, wherein the functional material is a ligand which specifically binds to the correspondent target cells. 4. A kit according to claim 3, wherein the ligand is selected from cell adhesion proteins, hormones, cytokines, antibodies. 5. A kit according to claim 4, wherein the cell adhesion protein is a cell binding domain polypeptide of fibronectin. 6. A kit according to claim 5, wherein the cell binding domain polypeptide of fibronectin is a polypeptide of the binding domain to VLA-5 and/or VLA-4. 7. A kit according to claim 4, wherein the ligand is erythropoietin. 8. A kit according to any one of claims 1-7, wherein the functional materials are immobilized. 9. A kit according to claim 4, wherein the functional material is immobilized on beads. 10. A kit for carrying out retrovirus-mediated gene transfer into target cells, which comprises: (a) an effective amount of a functional material having a target cell binding domain, and a retrovirus binding domain derived from a fibroblast growth factor, a collagen or a polylysine, or a functional equivalent thereof on the same molecule; (b) an artificial substrate for incubating the retrovirus and the target cells; and (c) a target cell growth factor for pre-stimulating the target cells. 11. A kit according to claim 10, wherein the fibroblast growth factor is selected from a fragment comprising fibroblast growth factor with an amino acid sequence represented by SEQ. ID No. 3 of the Sequence Listing, functional equivalents of the factor and polypeptides containing the factor or functional equivalent of the factor. 12. A kit according to claim 10, wherein the functional material is a polypeptide having an amino acid sequence represented by SEQ. ID No. 4 or 5 of the Sequence Listing. 13. A kit according to claim 10, wherein the collagen is selected from a fragment having insulin binding domain derived from type V collagen, functional equivalents of the fragment and polypeptides containing the fragment or functional equivalents of the fragment. 14. A kit according to claim 13, wherein the fragment having insulin binding domain derived from type V collagen is a fragment having an amino acid sequence represented by SEQ. ID No. 6 of the Sequence Listing. 15. A kit according to claim 10, wherein the functional material is a polypeptide having an amino acid sequence represented by SEQ. ID No. 7 or 8 of the Sequence Listing. 16. A kit according to any one of claims 10-15, wherein the functional material is immobilized.