Patent SG-11201909568U-A (SG)
Title: Reprogramming of a differentiated cell to an undifferentiated cell using exosome
Inventor: NARAYANAN KARTHIKEYAN
IPC: C12N5/0735
Filed: 2018-09-07
Abstract: MeanSize (nm) iPSC+75 118 + 75 HUE 5 7 119 + 68 Repro ramming.„ es Stem cells Stem cells: Uitracentrifugatir n A Fibroblasts FIG. 1 1-1 , re kr) O O cr O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau 111111111111110111111111111111101111101001111111111111111111111111101111111011110111111 (10) International Publication Number (43) International Publication Date WO 2019/050482 Al 14 March 2019 (14.03.2019) WIPO I PCT (51) International Patent Classification: C12N 5/0735 (2010.01) A61P 25/28 (2006.01) A61K 35/12 (2015.01) G01N 33/15 (2006.01) (21) International Application Number: PCT/SG2018/050457 (22) International Filing Date: 07 September 2018 (07.09.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201707334T 08 September 2017 (08.09.2017) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10, Connexis North Tower, Singapore 138632 (SG). (72) Inventors: NARAYANAN, Karthikeyan; c/o Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). DU, Chan; c/o In- stitute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). WAN, Andrew Chwee Aun; c/o Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (54) Title: REPROGRAMMING OF A DIFFERENTIATED CELL TO AN UNDIFFERENTIATED CELL USING EXOSOME (57) : The present invention generally relates to methods of reprogramming cells using exosomes derived from stem cells. The present invention also relates methods of screening drugs suitable for cancer treatment using the reprogrammed cells, obtained from the methods described herein. The present invention also relates to therapeutic uses of the reprogrammed cells, obtained from the methods described herein. [Continued on next page] WO 2019/050482 Al HINIONOIMEIMEME10 1 001 11111 IMEEMOIIIMEMEMIE TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: with international search report (Art. 21(3)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE