Patent AU-2020100181-A4 (AU)
Title: Stable Prebiotic Compositions
Inventor: MCKEE BENJAMIN ALEXANDER
IPC: A23L33/125
Filed: 2020-02-05
Granted: 2020-03-12
Abstract: STABLE PREBIOTIC COMPOSITIONS Abstract There is provided a stable prebiotic composition comprising 0.5 mg/g to 20 mg/g of nigerose; and a total concentration of 20 mg/g to 250 mg/g of trehalose, nigerose, maltulose, isomaltose, panose, turanose, kojibiose and palatinose, the total concentration of trehalose, nigerose, maltulose, isomaltose, panose, turanose, kojibiose and palatinose is stable for a period of six months or more at room temperature and/or stable for at least 12 hours at a temperature of 40°C, 50°C, or 70°C and the stable prebiotic composition increases Lactobacilli and/or Bifidobacteria levels and reduces Clostridia levels in the intestine of an animal. 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II 6OZZ3 ---- 8O ZZ3 I OZZ3 9OZZ3 tOzz:) 9OZZ3 90ZZ3 tozn: I I I: IOZZ3 I OZZ3 leil~ulTOZZ3 4) L .I1m 9t,/9 cu 0) z o 0 4-. rur U-U 0) m to F 0) z a z z .9 C., S C., (N N r S r z 'U '-I 0) r U) U) C) C) it it 01 zoulu fl!+ HV II [ £TH 0 0 VH, 8ETOZ:J I 6IETOZO LETOZO t7ETOZ3 E ETOZJ) OETOZJ) 6ZTOZ:J 8 ZTOZ:J L ZTOZJ) 9ZTOZJ) s ZTOZJ) ZZTOZ:J TZTOZ:J eI!l Lon 8Lon 8LCR 8Lon 6 .4/, O<) ex *muuu C> r~e'~i-~v (N0 - L 9t4101 0) Si TO I iO I I I STOI) 9Z10) sil0) N10) U TO Li I I 910) I 510) r014) ;0, eo -oo 46- 0000 0 " 0,I A 44,5 Ito ' 11%1 0z 0% 8888808 , L 9t7IC0 Iesoune1 osouqljepv IGSOJ!ZpNJ suouun ww U-soq~~ Scu .4-# asouedj asoiapueaqi RsOIJ3 sa umou)~uf asoliiewosi asoiqoiwa!E9 asou!leled asoinleqaiJ. asoiqIfo)i - - asolievq - - a5ouejnIL asO.1RIN ;aso!8uiwel asoiqoflaJ asoiqolnul asoleqajig'e asoleqajL-e'e co Lf 0 iJn 0 Ln U- * U asouedj asoi apueaqiL asoIJ3 souMOleUf ~soq umoumu asoi.lewosi asoiqo!4uag I- - - solle~A I~ - - souejni_ asOJ82!N - ~ asoinileviN asoiqjeuiwe-I asolqoIIaD asoiqolnul asoleLIaJ1-Ha'e asoieqaIIL-e'e 0M C Ljn C Lfl LL- Figure 20 Figure 21 Kojibiose Maltulose Erlose 30 abTrehalose 20 Isomallose 10 Trehalulose Nigerose Sucrose Maltose Turanose Q~~~J(DC C Ai( OJ~. x J N 0 O I~ 1 0 1 A~,A~pepojqa0 (N0 (0N a)e I-0 8t7I80 t~ -- oe -go ----- ~ 6 0 U ---.5 0 A - I. - -- .... l-l U-- wx 4% aa) 'P-t .Ir 8t70O ---- )4 * - - - A H--~k ii __ - - - o - - ,. -- A 0 -,, -* - - o~ I-c I.-to -- to --- I--z ---- x -- 05 I --- <O- t I --- - - * --- o 4 u P- * --- o ___~ ~ 6 *__ __0-, LFI 0 i Nr ' agdwes jad901nn U- 9t,/ I-z - 4% Coo 0 Or a)m I-0 8t7'to .I0r '4 '-I Lf, 0-4 -I 0 0 0 00 C0 0 qCT r4 6 o w C3 U- tn 0LA! 4-. oo L I- 9t7It7n in.nIrtnL d~o x C m 00 r-4 00 P4iC Ln 0 0 0 0 0 0 0 0 0 0 NO 0 NW 0~ N U- x ot 4Q%4 SMU S.0910 Iet Cyl0 ___ __ _ ___ __ ___ ___ L 12<1 all 0 r, tZ L Ilz m C4 4 A~!I.E....q8 Figure 32 50 -Maltulose 45- grs 40 - Tiknq N 0 Nr r-i 0:-meJf N f~Y 0nm o mi o -i 00 t fD~-I r-i'nt-'j Niemid) U) wr-~r-l*trmmi _1 _1 U UU U UUUUU U CJ UU U Figure 33 y =0.4943x -0 115 -10 0Y> 0 -Matulose -Combined T+K Linear (Maltulose) Linear (Combined T+K) Figure 34 18.00R' = 0.9711 180.00 120.00 •Maltulose 120.00 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00 Figure 35 R'= 0,7634 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00 Figure 36 200 *Nigerose *y=10.856X - 7.8848 a+ 2 4 6 8 10 12 14 16 -s0 Figure 37 250 *Nigerose 200 y .22x+3 R=0.5283 100 49, 0 2 4 6 8 10 12 14 16 Figure 38 0 Bifiobactria 0LactobacillimCotda Figure 39 Total anaerobes 5 Bifidobacteria 3 Clostridia Enteics 4) ifn a 0 Cuu 4) o E Sw o rC - (o O 't mO 0 ' Inci I I I I I I I II I I 7 --r I o D 0) 0 N- (D t '0 6 mC - C v ( nl~ojo ruio Bo U)U .4.i F- F -12 m2 0> 03 r- o L m~ofoi -oB 9t4 61 m F- m I 4a Ca ~. j R U! 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ED a) I I I I I I 9SUOdS9~ D~ H ~b t L 5 StdSS asuodseH asuodseH Figure 44 (A) 1.5 Cotia 1 F-1 __ LU.: In vivo -0.5 (B) 5 __________ *Lactobacilli o Bifidobacteria 3 *Clostridia U Whole 0-0 Hone Hone Hone Hone In vitro -2 -3 -4 A 00,0 -V) am 9% L) N N r'I Lf) jwnp So gtl/9% Ir czmIr __ __ ~, 4 ____ - #/ T <49 _ _ _ Oe, -_____-9 L-K-- rv4 ___K11so 4j FF1 'I' Lii 0 ~gliiiI ~: t'L OL 9 9 1' 9L 9 o 0 I ____I 0?' 0~ 0~ OL 9L 9 9?~ 9~ 9L 90 I~) I 43 I o I 0 I- 'I 1= U] 9~ 9L 90 9~0~9L0L 9 9 I' ~ 0 a) L 8t710t7 Sr 200O 90'0 90'0 t'O T~1l 917 'C GZ 9'L oo 09 ZL 0 co) F-4 -4 -4 vr -4 -T cn -4 -4 -4 (CN -4 -- (14 reN (3/aw LIO~ejzua)U-4 .L-z LL* - 9t/t Figure 50 (A) 200 Analysis 1(0 months) R= 0.9202 a100 7 7.5 8 8.5 9 9.5 Prebiotic Activity (B) 200 Analysis 2 (12 months) 140 -R2 =0.8198 140 Prebiotic Activity Ltn Cffn C, 0 m CU m w- .. 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