Patent SG-11201907566T-A (SG)
Title: Methods and compositions for labeling cells
Inventor: BOUTET STEPHANE CLAUDE
IPC: C12Q1/6816
Filed: 2018-12-07
Abstract: 8 1005 1006 1001. 1002 II 1026 1021 1027 1028 1029 1030 onl I ta, 4 LW 1 i2 ,- ,s5o , s, L.QPN.ffnnfxy$m**4* 10 1 24 1023 1025 1031 102; FIG. 10 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 13 June 2019 (13.06.2019) WIPO I PCT 11111111111110111111111111111101111101011111111111111111111111111110011111011110111111 (10) International Publication Number WO 2019/113533 Al (51) International Patent Classification: Cl 2Q 1/6816 (2018.01) (21) International Application Number: PCT/US2018/064600 (22) International Filing Date: 07 December 2018 (07.12.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/596,557 08 December 2017 (08.12.2017) US 16/107,685 21 August 2018 (21.08.2018) US 62/723,960 28 August 2018 (28.08.2018) US (71) Applicant: 10X GENOMICS, INC. [US/US]; 7068 Koll Center Parkway, Suite 401, Pleasanton, California 94566 (US). (72) Inventors: BOUTET, Stephane Claude; 7068 Koll Cen- ter Parkway, Suite 401, Pleasanton, California 94566 (US). LUCERO, Michael Ybarra; 7068 Koll Center Parkway, Suite 401, Pleasanton, California 94566 (US). MIKKELSEN, Tarjei Sigurd; 7068 Koll Center Parkway, Suite 401, Pleasanton, California 94566 (US). PFEIFFER, Katherine; 7068 Koll Center Parkway, Suite 401, Pleasan- ton, California 94566 (US). (74) Agent: YANDELL-ZHAO, Margaret; Wilson Sonsini Goodrich & Rosati, PC, 650 Page Mill Road, Palo Alto, Cal- ifornia 94304 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (54) Title: METHODS AND COMPOSITIONS FOR LABELING CELLS M M M (57) : The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing 0 of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell. N C [Continued on next page] WO 2019/113533 Al I 0111 111111110111111 0111 11112101 II 111 DIII OM VIII VIII OM II 0 1111111111111111 UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))