baber/WOPTO huggingface dataset:
Patent IT-MN920022-A1 (IT)
Title: Bracciale per sfigmomanometro polivalente-unificato che consente la  duplice modulazione in lunghezza ed ampiezza delle camere d'aria
Inventor: AGOSTA ROBERTO
Filed: 1992-06-26
Abstract: Il bracciale proposto trova applicazione nella pratica clinica medico-chirurgica di rilievo dei valori pressori arteriosi sistemici. Gli attuali manicotti per sfigmonanometro non sono modificabili in ampiezza e lunghezza e inducono la necessità, da parte dell'operatore, di dover sempre disporre contemporaneamente di tre diversi esemplari per adulto con ampiezza e lunghezza specificamente differenziate per braccia di piccola, normale e grande circonferenza, creando difficoltà mai risolte di dotazione, ingombro e memorizzazione. Il dispositivo proposto (Tavola 1) è in grado di fornire una radicale soluzione alla suddetta problematica, in quanto realizza un bracciale polivalente-unificato, dotato di due morsetti metallici con presse comandate da leve rotanti di 180°, sovrapposti e separabili (Tavola 5), i quali, facendole scorrere attraverso le loro due fessure, servono tre fasce: superiore, media e inferiore (Tavola 2); queste, dotate ciascuna di un manicotto di gomma appositamente dimensionato in ampiezza e lunghezza, possono essere separate o rese comunicanti mediante due brevi tubi di gomma X e V muniti di valvola (Tavola 1 e 3), realizzandosi così la possibilità di modulare sia la lunghezza che la ampiezza del dispositivo il quale, di volta in volta, può trasformarsi in bracciale per braccia piccole, normali e grandi risolvendo gli attuali problemi di dotazione, ingombro e memorizzazione e consentendo anche alle camere d'aria di effettuare, nell'ambito dell'adulto, il completo avvolgimento di tutta la gamma di circonferenze di braccia in esame, con riduzione del controproducente attuale fattore di dispersione pressoria e in condizioni ottimali di funzionalità prestazionale dei manicotti.
Patent IT-RM920548-A1 (IT)
Title: Dispositivo tergicristallo per autoveicoli
Inventor: HONER GERHARD
IPC: B60S1/60
Filed: 1992-07-17
Abstract: Un dispositivo di tergicristallo per autoveicoli, in particolare per i vetri di copertura di fari di autoveicoli, con un tergicristallo oscillabile da una posizione di servizio per la pulitura, in cui la sua spazzola è orientabile lungo una superficie del vetro del lato esterno dell'autoveicolo, in una posizione di riposo modificata rispetto alla posizione di servizio, e con una fascia di copertura che copre il tergicristallo, quando questo è in una posizione di riposo, verso il lato esterno del veicolo e che, quando il tergicristallo si trova in - posizione di servizio, libera una via per il movimento oscillatorio della spazzola, è caratterizzato dal fatto che con il movimento del tergicristallo dalla posizione di riposo nella posizione di servizio può essere mosso un listello di sostegno su cui poggia la spazzola del tergicristallo e dal fatto che il listello di supporto presenta, nella posizione di servizio, un sovrappasso livellato con la superficie del vetro.
Patent IT-RM940838-A1 (IT)
Title: "cerotto per assorbimento transcutaneo a rilascio controllato di estratto vegetale"
Inventor: VERARDI STEFANO
IPC: A61L/
Filed: 1994-12-23
Abstract: Cerotto per assorbimento transcutaneo a rilascio controllato, caratterizzato dal fatto di comprendere in combinazione le seguenti parti:-	uno strato adesivo puro di collante;-	uno strato di estratto idroalcolico di Ginseng e di eccipiente, reso adesivo dalla presenza di un collante; e -	un supporto sottile, flessibile, non repellente nei confronti del materiale adesivo, essendo eventualmente presente sullo strato adesivo puro uno strato protettivo asportabile, eventualmente dotato di una rima pretagliata centrale (onde facilitarne l'asportazione).
Patent IT-TO910521-A1 (IT)
Inventor: FRANCIONI RENZO
IPC: B65B/
Filed: 1991-07-04
Abstract: The articles (B) in an input flow are diverted onto a certain number of superposed sliding planes (5, 6, 7). This is achieved by means of one or more wheels, the peripheries of which have segments (10, 11, 12) which are spaced from the axis of rotation (X9) of the wheels (9) by distances corresponding to the distances of the sliding planes from the axis of rotation (X9). Each article is thus transferred towards a respective sliding plane (5, 6, 7) by a gradual movement. An alignment conveyor (16) located further downstream vertically aligns groups of articles on the superposed sliding planes (5, 6, 7) to define the piles. The piles are formed as a result of the articles (B) which are on the upper sliding planes (6, 7) falling onto the article below. The piles are preferably formed immediately before they are inserted in their packages (F). <IMAGE>
Patent IT-TO940883-A1 (IT)
Title: Materasso a molle magneto pulsore terapeutico.
Inventor: PASCIUTI FRANCO MARIO
IPC: A61N/
Filed: 1994-11-09
Abstract: Il materasso per mezzo di un pannello composto da un tracciato di metallo, buon conduttore, posto all'interno del materasso, emana dei segnali magneto pulsori. I segnali pervengono al pannello da una apparecchiatura elettronica.La finalità è di concorrere a curare stati infiammatori e stati dolorosi del nostro corpo con il segnale magneto pulsore durante il sonno o il riposo;
Patent IT-TO950100-A1 (IT)
Inventor: GENTA ALESSANDRO
IPC: H01R13/436
Filed: 1995-02-14
Abstract: An electric connector (1) presenting an insulating casing (2) with a number of axial cavities (3); a number of electric terminals (4) housed in the cavities (3); primary retaining means (18) for retaining the terminals (4) inside the cavities (3); secondary retaining means (25) in turn presenting a first movable element (26) connectable in a closed position to the casing (2); and a second element (33) interposed between the first element (26) and the casing (2) and movable between a disabling position preventing connection of the connector (1) to a complementary connector (1'), and an enabling position permitting such connection; the first element (26) presenting a pair of cam tabs (31) cooperating with the second element (33) so as to move it into the enabling position when the first element (26) is in the closed position.
Patent IT-TO970873-A1 (IT)
Inventor: GIULIANI ADRIANO
IPC: F16H61/18
Filed: 1997-10-02
Abstract: A control device for a gear change of a vehicle, in particular a commercial vehicle, provided with a casing (2), a control member (4) accommodated inside the casing (2) so as to be able to travel along its axis (A) to carry out a selection operation in the range of gears and occupy at least two selection positions (S4,S5), and so as to be able to rotate about said axis (A) to carry out a gear engagement/disengagement operation, with a first spring (49) interposed between the casing (2) and the control member (4) and adapted to exert along the axis (A) a first force (B1) on the control member (4) itself so as to bring it into abutment with a first stop means (11) defining a first (S5) of said selection positions (S4,S5), with a second spring (50) adapted to exert on the control member (4) a second force (B2) opposing the first force (B1) to displace the control member (4) itself towards a second stop member (31) defining a second (S4) of said selection positions (S4,S5), with a pneumatic cylinder co-operating with the spring (49,50) to counteract selectively the first and second forces (B1,B2) and bring the control member (4) in a stable manner respectively in the second selection position (S4) and in the first selection position (S5), and with a timed control unit (5) for controlling the pneumatic cylinder (5). <IMAGE>   <IMAGE>
Patent IT-VE930024-A1 (IT)
Title: Dispositivo per l'azionamento automatico di portelli di macchine  lavatrici centrifugatrici e simili
Inventor: OTTOLIN LUIGI
IPC: D06F/
Filed: 1993-08-06
Abstract: Dispositivo per l'azionamento automatico di portelli di macchine lavatrici, centrifugatrici e simili comprendenti un cesto rotante attorno ad un asse orizzontale all'interno di un cilindro fisso, caratterizzato dal fatto che:- un bordo del portello, parallelo alle generatrici del cesto, e il bordo corrispondente dell'apertura del cesto sono articolati fra di loro,- il bordo opposto del portello è impegnabile amovibilmente nel corrispondente bordo dell'apertura del cesto,- il portello può ruotare attorno all'articolazione solamente nello spazio interno al cesto ed è provvisto di mezzi per comandare tale rotazione,- l'operazione di scarico del materiale avviene facendo ruotare il cesto nel verso secondo il quale il bordo amovibile del portello precede quello articolato.
Patent NO-20111285-A1 (NO)
Title: System og fremgangsmate for styring av tralfiske
Inventor: SKJOLD-LARSEN HENNING
IPC: G01S11/00
Filed: 2011-09-22
Abstract: Et system for styring av trålfiske omfatter to eller flere sensorer for å måle trålparametre under fiske, en prosessorenhet for prosessering av sensordata en skjerm for fremvisning av sensordata og/eller prosesserte sensordata. En fremgangsmåte for styring av trålfiske omfatter måling av to eller flere trålparametre under fiske, beregning av trålparametrenes avvik fra ønsket verdi, og visning av avviket på en skjerm.
Patent NO-20180757-A1 (NO)
Inventor: DONDERICI BURKAY
IPC: G01V99/00
Filed: 2018-06-04
Abstract: A logging system includes an electromagnetic logging tool that collectsat least one measurement of a subterranean formation at a borehole. The logging system also includes a processing system that generates a plurality of earth models of the subterranean formation based on the at least one measurement of the subterranean formation. The processing system projects at least one second earth model upon a well path of the borehole, and selectsa model of the plurality of models based on a spatial correlation between at least one parameter of the selected model and a corresponding at least one parameter of the at least one second earth model.
Patent PE-39499-A1 (PE)
Title: Almohadilla absorbente elastizada
Inventor: DATTA PAUL JOSEPH
IPC: B32B5/26
Filed: 1998-01-14
Abstract: SE REFIERE A UNA ALMOHADILLA ABSORBENTE QUE DEFINE UN EJE LONGITUDINAL Y UN EJE TRANSVERSAL Y QUE COMPRENDE: A) UNA CAPA INFERIOR; B) UNA CAPA SUPERIOR PERMEABLE AL LIQUIDO SUPERPUESTA EN LA CAPA INFERIOR; C) UNA PIEZA ABSORBENTE COLOCADA ENTRE (A) Y (B); Y D) REBORDES LATERALES EN POSICION TRANSVERSAL Y ORIENTADOS HACIA AFUERA DE LA PIEZA ABSORBENTE, DONDE CADA REBORDE LATERAL COMPRENDE UNA PARTE ELASTICA QUE ESTA AL MENOS PARCIALMENTE FUERA DEL PLANO DE LA PIEZA ABSORBENTE CUANDO LA ALMOHADILLA ABSORBENTE SE ENCUENTRA POR LO GENERAL EN POSICION PLANA. ADEMAS DICHA ALMOHADILLA ABSORBENTE TIENE UNA RIGIDEZ GURLEY MAYOR QUE 200 mg APROXIMADAMENTE. LA PRESENTE INVENCION TIENE USO EN ARTICULOS ABSORBENTES TALES COMO ALMOHADILLAS, TOALLAS SANITARIAS Y SIMILARES
Patent PE-20001133-A1 (PE)
Title: Depresor de pirita
Inventor: ORTECHO ACOSTA DANIEL ROLANDO
IPC: B03D1/018
Filed: 1999-01-25
Abstract: ESTA REFERIDA A LA FABRICACION DE UN DEPRESOR DE PIRITA, UTILIZADO EN LA CONCENTRACION DE MINERALES POR FLOTACION. EL PROCEDIMIENTO PARA SU PREPARACION CONSISTE EN : i) DISOLVER EL NAFTENATO DE HIERRO, EN UNA CANTIDAD NO MAYOR DEL 15%, EN UN SOLVENTE ORGANICO, DE PREFERENCIA AGUARRAS; ii) A LO OBTENIDO EN (i) AGREGAR ALCOHOL ISOPROPILICO EN SIMILAR PROPORCION QUE EL OTRO SOLVENTE ORGANICO
Patent PE-52699-A1 (PE)
Title: Compuestos y metodos para preparar acidos 4-fenil-4-ciano-ciclohexanoicos sustituidos
Inventor: DIEDERICH ANN MARIE
IPC: C07C253/30
Filed: 1998-02-10
Abstract: SE REFIERE A UN METODO PARA PRODUCIR UN COMPUESTO DE FORMULA (I), DONDE R1 ES (CR4R5)nCOO(CR4R5)mR6, (CR4R5)n(CONR4), (CR4R5)rR6, ENTRE OTROS; SUSTITUIDOS OPCIONALMENTE CON UNO O MAS HALOGENOS; m ES 0-2, n ES 1-4, r ES 0-6, R4 Y R5 SON INDEPENDIENTEMENTE H O ALQUILO C1-C2; R6 ES H, METILO, OH, ARILO, ENTRE OTROS, SI R6 ES OH, m ES 2 o r ES 2-6 o SI R6 ES 2-TETRAHIDROPIRANILO, 2-TETRAHIDROFURANILO, ENTRE OTROS, m ES 1-2 o r ES 1-6; X ES YR2, HALOGENO, NH2 O FORMILAMINA; X2 ES O o NR8; Y ES O � S(O)m`, m' ES 0-2; R2 ES CH3, NO2 o CH2CH3 SUSTITUIDOS CON UNO O MAS HALOGENOS; R3 ES H, HALOGENO, ALQUILO C1-C4, -CH=CR8'R8', NR8R10, CH2NR8R10, C(Z')H, ENTRE OTROS; R8 ES H, ALQUILO C1-C4 SUSTITUIDO CON 1-3 F; R8' ES R8 o F; R10 ES OR8 o R11; R11 ES H o ALQUILO C1-C4 SUSTITUIDO CON 1-3 F; Z' ES O, NR9, NOR8, NCN, ENTRE OTROS; R'Y R'' SON H o COOH. EL METODO COMPRENDE TRATAR UN COMPUESTO DE FORMULA (II) CON BROMURO DE LITIO EN UN DISOLVENTE POLAR A UNA TEMPERATURA ENTRE 60�C-100�C OPCIONALMENTE EN UNA ATMOSFERA INERTE. TAMBIEN SE REFIERE A LOS COMPUESTOS INTERMEDIOS DE FORMULA (II)
Patent PE-20000014-A1 (PE)
Title: Triazolopurinas, procedimientos para su preparacion y su empleo como medicamentos
Inventor: KUMMER WERNER
IPC: C07D487/14
Filed: 1997-07-21
Abstract: SE REFIERE A COMPUESTOS QUE SE PUEDEN OBTENER AL REACCIONAR UNA AMINOGUANIDINA CON UN DERIVADO DE ACIDO CARBOXILICO, OBTENIENDOSE UN TRIAZOL, EL QUE LUEGO REACCIONA CON UN ESTER ALQUILICO DE ACIDO CIANOACETICO FORMANDOSE UN ANILLO EN CONDICIONES ALCALINAS Y FORMANDOSE UNA TRIAZOLPIRIMIDINA. LOS COMPUESTOS OBTENIDOS ACTUAN COMO ANTAGONISTAS DE "ADENOSINA", NEUROMODULADOR ENDOGENO CON EFECTOS INHIBIDORES DEL SISTEMA NERVIOSO CENTRAL (SNC), RINONES Y OTROS ORGANOS, POR LO QUE SERIAN DE UTILIDAD EN EL TRATAMIENTO DE ENFERMEDADES DEGENERATIVAS COMO DEMENCIA SENIL DEL TIPO ENFERMEDAD DE ALZHEIMER, TRASTORNOS ASOCIADOS CON LA EDAD, DEPRESION; ENFERMEDADES NEURODEGENERATIVAS COMO ENFERMEDAD DE PARKINSON, JAQUECA, TRATAMIENTO DE DISNEA; ENFERMEDADES CARDIOVASCULARES COMO INSUFICIENCIA CARDIACA, DE FIBROSIS CISTICA (MUCOVISCOSIDAD)
Patent PE-26098-A1 (PE)
Title: Un nuevo derivado quinolina del acido carboxilico que posee un substituto 7-(4-aminometil-3-oxima) pirrolidina, y proceso para su preparacion
Inventor: KIM SE HO
IPC: C07D471/04
Filed: 1996-12-20
Abstract: SE REFIERE AL COMPUESTO DE FORMULA (I), EN DONDE: R ES H, METILO o AMINO; "Q" ES C-H, C-F, C-Cl o N, ENTRE OTROS; R1 ES PREFERENTEMENTE CICLOPROPIL o 2,4 DIFLUOROFENIL; R2 ES H, ALQUIL C1-C4, HOMOPROPARGIL, BENCIL SUSTITUIDO CON F, METILO o METOXI, o ES 2-FLUOROETIL, ENTRE OTROS; R3 Y R4 SON INDEPENDIENTEMENTE H, ALQUILO C1-C3 o FORMAN JUNTO CON EL NITROGENO UN ANILLO. TAMBIEN SE REFIERE AL PROCEDIMIENTO DE PREPARACION DE (I), QUE COMPRENDE: HACER REACCIONAR LA QUINOLINA DEL ACIDO CARBOXILICO SUSTITUIDA EN POSICION 7 CON UN HALOGENO, CON: a) UN COMPUESTO (III) EN PRESENCIA DE UN ACIDO ACEPTOR Y A UNA TEMPERATURA DE 200�C o b) UN COMPUESTO (III`) TAL COMO (III), EN DONDE: R3 Y R4 SON H Y UN GRUPO AMINO PROTECTOR, EN PRESENCIA DE UNA BASE. LOS COMPUESTOS OBTENIDOS SON ANTIBIOTICOS DE AMPLIO ESPECTRO, INCLUYENDO CEPAS RESISTENTES, QUE PRESENTAN PROPIEDADES FARMACOCINETICAS Y DE ABSORCION MEJORADAS
Patent PL-254034-A1 (PL)
Title: Method of continusly separating components of mixtures in serial flow
IPC: C02F1/26
Filed: 1985-06-17
Abstract: A process for separating an extract component from a raffinate component contained in a feed mixture. A unidirectional fluid flow system is maintained through a series of separating units through which the components travel at different rates. The units are interconnected in series so as to form a single closed loop. A component concentration distribution is established within the system of units so as to comprise a series of zones. Feed and displacement fluid are each mixed with a different intercolumn stream prior to being passed into the inlets of two of the units and extract and raffinate are taken only as portions of streams from outlets of two or more of the units at appropriate points on the component concentration distribution. At the appropriate times the inlets and outlets are shifted so as to simulate movement of the units in a direction downstream with respect to the direction of fluid flow and thereby enable the inlets and outlets to continually lie in the appropriate zones.
Patent PL-272768-A1 (PL)
Title: Method of obtaining barium sulfate of chemically reactive surface
IPC: C09C1/02
Filed: 1988-05-30
Abstract: There is described a process for preparing barium sulphate having a chemically reactive surface by precipitating barium ions in an aqueous medium by means of sulphate ions. 
<??>To make barium sulphate available as a filler having functional properties, the precipitation, or coprecipitation, is carried out in the presence of additional anions of water-soluble compounds which are precipitable with barium ions and form sparingly soluble barium compounds therewith. 
<??>The product, which contains foreign ions and is reactive, is subjected to a suitable aftertreatment for whichever particular use is envisaged.
Patent PL-298041-A1 (PL)
Title: Arrangement for closing a wall opening in particular an access opening to a network switching station
Inventor: PRIMUS ILLO-FRANK
IPC: H02B7/00
Filed: 1993-03-12
Abstract: A device for closing off a wall opening used for access to a switch cubicle embedded in concrete has a frame with soffit plate and a hinged door. The box section frame holds the hinge which has an arm projecting through the frame onto the rear of the door. The door has an opening angle of more than 90 degrees. The frame is made from extruded aluminium. Around its inner walls are shoulders which form location surfaces for the hinge unit, a tubular insert or a corner jointing piece. Alternatively, the frame for the hinge unit may be of two part form, a trough-shaped section abutting the concrete wall closed by an L-shaped piece. ADVANTAGE- Concealed hinges, easy waterproofing and full access to interior of cubicle.
Patent PL-313371-A1 (PL)
Title: Deformable, thermally stabilised open lattice structure
Inventor: DINGER ROLF
IPC: D04B21/12
Filed: 1996-03-20
Abstract: An open network structure of knitted or woven fabric, made of multifilament hybrid yarn contg. two sorts of filaments (A) and (B) in a wt. ratio of (20:80)-(80:20), pref. (40:60)-(60:40), and opt. up to 40 wt% other filaments (C). Component (A) consists of textured filaments with a m.pt. of more than 180 (pref. above 220, esp. pref. above 250) deg. C. (B) consists of filaments with a m.pt. of less than 220 (pref. below 200, esp. pref. below 180) deg. C, and the m.pt. of (B) is at least 20 (pref. at least 40, esp. pref. at least 80) deg. C below the m.pt. of (A). Also claimed is a process for the prodn. of the above thermally bondable structure by weaving or knitting smooth fabric or fabric with a relief pattern, in which the yarn which is supplied to the loom or knitting machine contains at least 30 (pref. at least 70) % yarn as above.
Patent PL-322282-A1 (PL)
Title: Magnetic, superficial damping element and glue in particular for application onto metal surfaces
Inventor: RUHE THOMAS
IPC: G10K11/162
Filed: 1996-03-26
Abstract: PCT No. PCT/EP96/01326 Sec. 371 Date Jan. 7, 1998 Sec. 102(e) Date Jan. 7, 1998 PCT Filed Mar. 26, 1996 PCT Pub. No. WO96/32709 PCT Pub. Date Oct. 17, 1996In a magnetizable flat damping component with an acoustic wave damping or suppressing layer (5) with an adhesive layer (2) bonded thereto for securing the damping component to other objects (6) and with magnetizable particles (3) in the damping component the magnetizable particles are integrated into the adhesive layer (2) using binding agents (4).
Patent PL-324459-A1 (PL)
Title: Improvement related to a liquid dispensing device
Inventor: BOLTON TERENCE WILLIAM
IPC: B05B7/24
Filed: 1996-03-21
Abstract: PCT No. PCT/EP96/01225 Sec. 371 Date Apr. 14, 1998 Sec. 102(e) Date Apr. 14, 1998 PCT Filed Mar. 21, 1996 PCT Pub. No. WO97/03757 PCT Pub. Date Feb. 6, 1997Apparatus for dispensing a fine spray of liquid particles comprises a hollow tubular casing (1) whose internal cross section is formed with at least one abutment surface. A tubular liquid source (2) is positioned within the casing (1) with the abutment surface in close contact with the outer surface of the source to provide support thereto. The casing (1) includes a passageway for the flow of air from a mouthpiece (3) positioned at one end of the casing (1) to a nozzle (9) positioned at the other end of the casing (1) into which the discharge end of the liquid source protrudes. The liquid source may comprise a pen formed with a nib (14) of absorbent material.
Patent PL-331418-A1 (PL)
Title: Short-circuit protection for semiconductor-type change-over switches
Inventor: SAIRANEN MARTTI
IPC: H03K17/08
Filed: 1998-06-09
Abstract: PCT No. PCT/FI98/00495 Sec. 371 Date Mar. 31, 1999 Sec. 102(e) Date Mar. 31, 1999 PCT Filed Jun. 9, 1998 PCT Pub. No. WO98/59421 PCT Pub. Date Dec. 30, 1998A power control (20, 40) for controlling the electric power fed from voltage source (12) to a load (13) comprises a field effect transistor (21, Q1) for either allowing or disabling the flow of current from voltage source to load as a response to the presence or absence of a control pulse led to the gate of the field effect transistor. Additionally the power control comprises means for measuring the change of the gate potential of the field effect transistor (24, R6, R7) and for either allowing or disabling a control pulse on the basis of the gate potential change measurement. In the power control the control pulse is allowed to switch the field effect transistor (21, Q1) to conducting state when a stop (31) in the voltage increase due to Miller effect is detected at the start of the control pulse, and the control pulse is respectively disabled from switching the field effect transistor to conducting state when no Miller effect stop (33) is detected in the field effect transistor gate potential change at the start of the control pulse.
Patent PL-333483-A1 (PL)
Title: Methods of and apparatus for making aluminium foil bags
Inventor: WILD HANS-PETER
IPC: B31B50/16
Filed: 1999-05-31
Abstract: The method involves transporting two foils (4,6) from which the sides of the bags will be formed. Holes are formed in a base foil (8) at intervals corresponding to the width of a bag while transporting it. The base foil is folded along the center line parallel to the transport direction (52), whereby the holes are arranged symmetrically with respect to the center line. Hole measurements are performed. The side foils and folded base foil are stacked. The foils are joined so that where the base foil is sandwiched between the side foils, the side foils are only joined directly to each other via the holes. The joined layers are cut so that a side edge joint region is divided between each two foil bags. An Independent claim is also included for an arrangement for implementing the method.
Patent PL-341681-A1 (PL)
Title: Method of obtaining complex fingerprints of dna methylation
Inventor: OLEK ALEXANDER
IPC: C12Q1/6886
Filed: 1998-11-27
Abstract: Method for characterizing, classifying and differentiating tissues and cell types, for predicting the behavior of tissues and groups of cells, and for identifying genes with changed expression. The method involves obtaining genomic DNA from a tissue sample, the genomic DNA subsequently being subjected to shearing, cleaved by means of a restriction endonuclease or not treated by either one of these methods. The base cytosine, but not 5-methylcytosine, from the thus-obtained genomic DNA is then converted into uracil by treatment with a bisulfite solution. Fractions of the thus-treated genomic DNA are then amplified using either very short or degenerated oligonucleotides or oligonuclcotides which are complementary to adaptor oligonucleotides that have been ligated to the ends of the cleaved DNA. The quantity of the remaining cytosines on the guanine-rich DNA strand and/or the quantity of guanines on the cytosine-rich DNA strand from the amplified fractions are then detected by hybridization or polymerase reaction, which quantities are such that the data generated thereby and automatically applied to a processing algorithm allow the drawing of conclusions concerning the phenotype of the sample material.