Patent SG-11201806794R-A (SG)
Title: Purification of rna fractions using a hydrophilic polymeric material
Inventor: LAUFER THOMAS
IPC: G01N1/40
Filed: 2017-02-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 21 September 2017 (21.09.2017) WIPO I PCT (10) International Publication Number WO 2017/157650 Al 111111111111110111011111111111010111110 0111111111111111111111111111111111111110111111 (51) International Patent Classification: G01N 1/34 (2006.01) GO1N 1/40 (2006.01) (21) International Application Number: PCT/EP2017/054582 (22) International Filing Date: 28 February 2017 (28.02.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 16161077.9 18 March 2016 (18.03.2016) EP (71) Applicant: HUMMINGBIRD DIAGNOSTICS GMBH [DE/DE]; Im Neuenheimer Feld 583, 69120 Heidelberg (DE). (72) Inventors: LAUFER, Thomas; Lessingstr. 17, 68535 Edingen-Neckarhausen (DE). BEIER, Markus; Marc- Aurel-Weg 20, 69469 Weinheim (DE). KAHRAMAN, Mustafa; Waldhausweg 7, 66123 Saarbriicken (DE). KELLER, Andreas; Albert-Schweitzer-Str. 6, 66346 Piit- tlingen (DE). (74) Agent: GEEING, Andrea; ZSP Patentanwalte PartG mbB, Hansastrasse 32, 80686 Miinchen (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) W O 20 17157 / 65 0 Al (54) Title: PURIFICATION OF RNA FRACTIONS USING A HYDROPHILIC POLYMERIC MATERIAL (57) : The present invention relates to a method of removing an RNA fraction with > 200 nucleotides in length from a whole blood sample. The present invention also relates to a method of purifying an RNA fraction with < 200 nucleotides in length from a whole blood sample. The present invention further relates to a method of determining the level of RNA molecules with < 200 nucleotides in length. In addition, the present invention relates to a method for diagnosing a disease in an individual. Moreover, the present invention relates to a kit which is useful for carrying out the methods of the present invention.