Patent SG-11201900895Q-A (SG)
Title: Assay for determining potential to self-association of a protein using concentration-dependent self-interaction nanoparticle spectroscopy
Inventor: MARLOW MICHAEL
IPC: G01N33/68
Filed: 2017-08-18
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) CORRECTED VERSION (19) World Intellectual Property Organization International Bureau (43) International Publication Date 22 February 2018 (22.02.2018) WIPO I PCT 11111111111101101111111111101101011 H 01100 1111101110# (10) International Publication Number WO 2018/035470 A9 (51) International Patent Classification: G01N 33/50 (2006.01) GO1N 33/68 (2006.01) (21) International Application Number: PCT/US2017/047630 (22) International Filing Date: 18 August 2017 (18.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/376,788 18 August 2016 (18.08.2016) US (71) Applicant: REGENERON PHARMACEUTICALS, INC. [US/US]; 777 Old Saw Mill River Rd., Tarrytown, New York 10591 (US). (72) Inventors: MARLOW, Michael; 11 Park Place, Apt. 409, New Rochelle, New York 10801 (US). SENNETT, Michael; 182 Powder House Blvd., Sommerville, Massa- chusetts 02144 (US). SCHNEIDER, Michael; c/o Regen- eron Pharmaceuticals, Inc., 777 Old Saw Mill River Rd., Tarrytown, New York 10591 (US). = (74) Agent: KIRSCH, Gregory J.; Smith Gambrell & Russell LLP, 1230 Peachtree Street, N.E., Suite 3100, Promenade, Atlanta, GA 30309 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (48) Date of publication of this corrected version: 05 July 2018 (05.07.2018) (15) Information about Correction: see Notice of 05 July 2018 (05.07.2018) O N O 00 O 0 (54) Title: ASSAY FOR DETERMINING POTENTIAL TO SELF-ASSOCIATION OF A PROTEIN USING CONCEN- TRATION-DEPENDENT SELF-INTERACTION NANOPARTICLE SPECTROSCOPY (57) : Methods for producing high concentration protein formulations having high stability are provided. Assays for selecting proteins and formulation conditions that have high self-repulsive attributes are used as an early step in the manufacturing process. Specifically, a protein concentration-dependent self-interaction nanoparticle spectroscopy method is employed as a protein colloidal interaction assay.