Patent SG-11201810040W-A (SG)
Title: Direct selection of cells expressing high levels of heteromeric proteins using glutamine synthetase intragenic complementation vectors
Inventor: KETCHEM RANDAL R
IPC: C12N15/67
Filed: 2017-05-11
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111111111111111111111111111111111111111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2017/197098 Al 16 November 2017 (16.11.2017) WIPO I PCT (51) International Patent Classification: Declarations under Rule 4.17: C12N 15/65 (2006.01) C12N 15/67 (2006.01) — as to applicant's entitlement to apply for and be granted a (21) International Application Number: patent (Rule 4.1700) PCT/US2017/032139 Published: (22) International Filing Date: — with international search report (Art. 21(3)) 11 May 2017 (11.05.2017) — with sequence listing part of description (Rule 5.2(a)) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/334,966 11 May 2016 (11.05.2016) US (71) Applicant: AMGEN INC. [US/US]; One Amgen Center Drive, Thousand Oaks, California 91320 (US). (72) Inventors: KETCHEM, Randal R.; 6332 152nd Street SE, Snohomish, Washington 98296 (US). MCGREW, Jef- frey T.; 19237 160th Avenue NE, Woodinville, Washing- ton 98072 (US). FOMINA YADLIN, Dina A.; 2324 42nd _ Avenue E, Seattle, Washington 98112 (US). MUNRO, Trent P.; 1915 Seabreeze Street, Thousand Oaks, Cali- - fornia 91320 (US). AGRAWAL, Neeraj Jagdish; 1610 = Camino Olmo Apt. K, Thousand Oaks, California 91320 (US). DARIS, Kristine M.; 1210 Rotella Street, Newbury = Park, California 91320 (US). — = Agent: ANDALIS, Alex A.; One Amgen Center Drive, Thousand Oaks, California 91320 (US). = (74) = (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, = HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, = = = PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = = = Designated States (unless otherwise indicated, for every (84) = kind of regional protection available): ARIPO (BW, GH, — GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, = UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, — 1-1 GC MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). 0\ 0 IN 0\ 1-1 (54) Title: DIRECT SELECTION OF CELLS EXPRESSING HIGH LEVELS OF HETEROMERIC PROTEINS USING GLU- IN TAMINE SYNTHETASE INTRAGENIC COMPLEMENTATION VECTORS 1-1 0 (57) : This invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More \" specifically, the invention concerns improved selection of cells transfected with recombinantly engineered vectors designed to express 0 polypeptides, in particular heteromultimeric polypeptides.