Patent SG-11201804510P-A (SG)
Title: Biparatopic polypeptides antagonizing wnt signaling in tumor cells
Inventor: ZINZALLA VITTORIA
IPC: C07K16/46
Filed: 2016-12-02
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau 111111111M 11111111111111111111111111111111111111111111111111111111111111R111111111111111 (10) International Publication Number WO 2017/093478 Al WIPO I PCT (43) International Publication Date 8 June 2017 (08.06.2017) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.170) Published: with international search report (Art 21(3)) with sequence listing part of description (Rule 5.2(a)) (51) International Patent Classification: C07K 16/28 (2006.01) C07K 16/46 (2006.01) A61K 39/00 (2006.01) A61P 35/00 (2006.01) C07K 16/18 (2006.01) (21) International Application Number: PCT/EP2016/079575 (22) International Filing Date: 2 December 2016 (02.12.2016) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 15197999.4 4 December 2015 (04.12.2015) EP (71) Applicant: BOEHRINGER INGELHEIM INTERNA- TIONAL GMBH [DE/DE]; Binger Strasse 173, 55216 In- gelheim Am Rhein (DE). (72) Inventors: ZINZALLA, Vittoria; Boehringer Ingelheim GmbH/ Corporate Patents, Binger Strasse 173, 55216 In- gelheim Am Rhein (DE). KUENKELE, Klaus-Peter; Boehringer Ingelheim GmbH/ Corporate Patents, Binger Strasse 173, 55216 Ingelheim Am Rhein (DE). BUYSE, Marie-Ange; Ablynx N.V. Technologiepark 21, 9052 Zwijnaarde (BE). CROMIE, Karen; ABLYNX N.V Tech- nologiepark 21, 9052 Zwijnaarde (BE). STAELENS, Stephanie; Ablynx N.V. Technologiepark 21, 9052 Zwijn- aarde (BE). STRUBBE, Beatrijs; Ablynx N.V. Technolo- giepark 21, 9052 Zwijnaarde (BE). (74) Agents: SIMON, Elke et al.; Boehringer Ingelheim GmbH, Corporate Patents, Binger Strasse 173, 55216 In- gelheim Am Rhein (DE). W O 20 17 /093 47 8 Al (54) Title: BIPARATOPIC POLYPEPTIDES ANTAGONIZING WNT SIGNALING IN TUMOR CELLS (57) : The invention provides novel biparatopic LRPS/LRP6 cross-reactive binding polypeptides, and more specifically novel biparatopic LRPS/LRP6 cross-reactive immunoglobulin single variable domain constructs which can inhibit Wnt signaling pathways. The invention also relates to specific sequences of such polypeptides, methods of their production, and methods of using them, including methods of treatment of diseases such as cancer.