Patent SG-11201804001R-A (SG)
Title: A cell culture substrate and method of making thereof
Inventor: YING JACKIE Y
IPC: C12N5/0735
Filed: 2016-11-30
Abstract: emenc. Nan.opanki () INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau 11111111111111111111111111111111111111111111111111111111111111111111R111111111111111 (10) International Publication Number WO 2017/095333 Al WIPO I PCT (43) International Publication Date 8 June 2017 (08.06.2017) (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12N 5/0735 (2010.01) kind of national protection available): AE, AG, AL, AM, (21) International Application Number: PCT/SG2016/050587 AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (22) International Filing Date: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 30 November 2016 (30.11.2016) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (26) Publication Language: English TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (30) Priority Data: ZW. 10201509845Q 30 November 2015 (30.11.2015) SG (84) Designated States (unless otherwise indicated, for every (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY kind of regional protection available): ARIPO (BW, GH, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Connexis, Singapore 138632 (SG). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (72) Inventors: YING, Jackie Y.; c/o Institute of Bioengineer- DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, ing and Nanotechnology, 31 Biopolis Way, The Nanos, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, #04-01, Singapore 138669 (SG). ERATHODIYIL, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Nandanan; c/o Institute of Bioengineering and Nanotech- GW, KM, ML, MR, NE, SN, TD, TG). nology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). NARAYANAN, Karthikeyan; c/o Institute Declarations under Rule 4.17: of Bioengineering and Nanotechnology, 31 Biopolis Way, — of inventorship (Rule 4.17(iv)) The Nanos, #04-01, Singapore 138669 (SG). WAN, An- drew C. A.; c/o Institute of Bioengineering and Nanotech- Published: nology, 31 Biopolis Way, The Nanos, #04-01, Singapore with international search report (Art 21(3)) 138669 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (54) Title: A CELL CULTURE SUBSTRATE AND METHOD OF MAKING THEREOF [Fig. 1 1 W O 20 17 /095 333 Al a Cross-linker Arise-11(511011e1 Acrylamide monomer 111 C41 reverse inic.rDernuisi.on Stabilizati-DEI.v.eqn - .5;i:fac.1 - at?rs 2. Pelynferizattrir - , 3 Fur:tr.:Ina , : (57) : A cell culture substrate comprising a substrate having a coating of a plurality of amine functionalized nanoparticles is disclosed. In one embodiment, the amine functionalized nanoparticle is a polymer of an acrylamide monomer, a cross -linker and an amine monomer. There is also provided a method of making the cell culture substrate either by drying the amine functionalized nan- oparticles when spread onto the said substrate or by covalent linkages of the substrate with thiol teiininated nanoparticles. In addi- tion, there is provided a method of culturing stem cells on the cell culture substrate having a coating of a plurality of the amine func - tionalized nanoparticles thereon.