Patent RU-2651073-C2 (RU)
Inventor: Novoseltsev Aleksej Yurevich
IPC: A61M1/34
Filed: 2016-07-22
Granted: 2018-04-18
Abstract: FIELD: medicine and veterinary medicine. 
     SUBSTANCE: invention relates to the field of medicine, namely to veterinary medicine. Vein puncture is performed, an exfusion phase, on which a portion of blood is taken, a dose of an anticoagulant and a diluent from the reservoirs containing them is introduced therein, and the reinfusion phase on which the resulting exfusate is returned to the vein through a plasma filter with the simultaneous separation of plasma on it and cyclic repetition of the exfusion and reinfusion phases in which a portion of blood, the dose of anticoagulant and diluent is introduced into the straightening elastic chamber of the pump segment, which is previously compressed into the reinfusion phase. Volume of the exfusate is set by limiting the degree of straightening of the chamber of the pump segment, after which the reverse current of the anticoagulant and diluent in the decreasing containers and the exfusate in the vein and the plasma into the plasma filter is blocked in the beginning of the reinfusion phase. In this case, the initial clotting time of the animal's blood is predetermined before the start of the procedure. Calculate the dose of anticoagulant and inject it intravenously before the procedure, and for the catheterization of the vein, the femoral or jugular vein, the central catheter is placed, or a peripheral catheter is placed on the subcutaneous vein of the thigh or shoulder of the animal. In this case, instead of the removed plasma, the saline solution is introduced, the volume of which is calculated depending on the degree of hydration of the animal, and during the procedure during the phase of exfusion, an anticoagulant is additionally administered. In addition, before catheter insertion in a vein, catheters are washed with an anticoagulant solution, and immediately after catheter insertion into the vein, the catheter is washed with saline and filled with an anticoagulant, forming an anticoagulant plug, the blood draw and recovery are regulated by an anti-recirculation valve, blood flow is regulated during the exfusion, and infusion of a solution of calcium gluconate is carried out after the termination of the procedure plasmapheresis. In a special case, when the catheter is inserted into the jugular vein, the central venous pressure is measured and the amount of saline compensation is calculated based on the measurement result. Bloodline is blocked so that the phase of exfusion lasts 1 second. In particular, for small animals, the blood draw is regulated by manually clamping the return bloodline. Device for carrying out the method of plasmapheresis includes a blood draw and recovery means, an exfusion bloodline comprising a clamp and a pump segment having an elastic chamber with inlet and outlet nozzles. Reinfusion bloodline containing a plasma filter, an air trap with a filter and a clamp. Both said lines and the blood draw and recovery means are connected via a tee, a plasma outlet line connected to a plasma filter and comprising a clamp and a plasma reservoir, a pressure measurement line connected through a tee with a reinfusion bloodline and an exfusion bloodline. At the same time, check valves are built in the exfusion bloodline, the reinfusion bloodline, the anticoagulant supply line, the diluent supply line and the plasma outlet line, an anticirculation valve is built in the reinfusion bloodline after the air trap, means for controlling the lumen is installed on the exfusion bloodline before the pump segment, and the anticoagulant supply line and the diluent supply line are connected to the exfusion bloodline. 
     EFFECT: method and device allow to optimize the parameters of plasmapheresis, to increase its reliability and safety of use in animals, including the procedure for animals with a low body weight. 
     5 cl, 2 dwg, 2 ex