Patent SG-11201809617R-A (SG)
Title: Intracellular delivery of biomolecules to induce tolerance
Inventor: LOUGHHEAD SCOTT
IPC: A61P37/02
Filed: 2017-05-03
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111101111 0111010101111101011111 0 1110111110111101H11111111111111111111110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\" WO 2017/192786 Al 09 November 2017 (09.11.2017) W I P0 I P C T (51) International Patent Classification: EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, A61K 35/17 (2015.01) A61P 37/02 (2006.01) MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, A61K 35/15 (2015.01) TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (21) International Application Number: PCT/US2017/030933 Published: (22) International Filing Date: — with international search report (Art. 21(3)) 03 May 2017 (03.05.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/331,384 03 May 2016 (03.05.2016) US (71) Applicant: SQZ BIOTECHNOLOGIES COMPANY [US/US]; 134 Coolidge Avenue, Watertown, MA 02472 (US). (72) Inventors: LOUGHHEAD, Scott; c/o SQZ Biotechnolo- gies Company, 134 Coolidge Avenue, Watertown, MA 02472 (US). GILBERT, Jonathan, B.; c/o SQZ Biotech- nologies Company, 134 Coolidge Avenue, Watertown, MA 02472 (US). BERNSTEIN, Howard; c/o SQZ Biotech- nologies Company, 134 Coolidge Avenue, Watertown, MA 02472 (US). SHAREI, Armon, R.; c/o SQZ Biotechnolo- gies Company, 134 Coolidge Avenue, Watertown, MA 02472 (US). MOORE, Finola; c/o SQZ Biotechnologies Company, 134 Coolidge Avenue, Watertown, MA 02472 (US). Agent: VANEVSKI, Filip et al.; Morrison & Foerster LLP, 755 Page Mill Road, Palo Alto, CA 94304-1018 (US). = (74) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (81) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (84) 1-1 SO GC Ir ---- (54) Title: INTRACELLULAR DELIVERY OF BIOMOLECULES TO INDUCE TOLERANCE ei C:N (57) : The present invention provides methods for inducing tolerance or suppressing an immune response to an antigen by 1-1 ----. passing a cell suspension containing an immune cell through a constriction, wherein the constriction deforms the cell thereby causing a IN , 1 perturbation of the cell such that an antigen and/or tolerogenic factor enters the cell, thereby generating a tolerogenic and/or immuno- © suppressive immune cell. In some embodiments, the tolerogenic and/or immunosuppressive immune cell is delivered to an individual N and presentation of the antigen induces tolerance and/or suppresses an immune response to the antigen. C