Patent EA-005290-B1 (EA)
Title: A method of diagnosing or prognosticating hiv infection in a subject
IPC: G01N33/569
Filed: 2000-11-27
Abstract: 1. A method of diagnosing and/or prognosticating HIV infection in a subject comprising the steps of: (a) performing in vitro a measurement of the level of one or more markers in the form of (i) urokinase plasminogen activator receptor (uPAR), and/or (ii) soluble urokinase plasminogen activator receptor (suPAR), and/or one or more degradation products of (i) or (ii) and (b) using the measurement value obtained to evaluate the state of the subject. 2. A method of diagnosing and/or prognosticating HIV infection in a subject comprising the steps of: (a) performing in vitro a measurement of the level of a marker in the form of (i) urokinase plasminogen activator receptor (uPAR), and/or (ii) soluble urokinase plasminogen activator receptor (suPAR), or (iii) one or more degradation products of (i) or (ii), and (b) using the measurement value obtained to evaluate the state of the subject. 3. A method according to claims 1 or 2, wherein the biological fluid sample is a urine sample, and wherein the measurement of the marker is correlated to the total concentration level of the sample. 4. A method according to any one of claims 1-3, wherein step a) is effected by means of a stick. 5. A method according to any one of claims 1-3, wherein step a) is effected using ELISA. 6. A method of evaluating the progression of the state of a subject suffering from HIV infection comprising the steps of: (a) performing in vitro a measurement of the level of one or more markers in the form of (i) urokinase plasminogen activator receptor (uPAR), and/or (ii) soluble urokinase plasminogen activator receptor (suPAR),and/or (iii) one or more degradation products of (i) or (ii), in each of a number of biological fluid samples from a subject, wherein the samples are obtained at different points in time, and (b) using the measurement values obtained to evaluate the progression of the state of the subject. 7. A method of diagnosing and/or prognosticating HIV infection in a subject comprising the steps of: (a) performing in vitro a of the level of a marker in the form of (i) urokinase plasminogen activator receptor (uPAR), and/or (ii) soluble urokinase plasminogen activator receptor (suPAR), or (iii) one or more degradation products of (i) or (ii), ii), measurement in each of a number of biological fluid samples from a subject, wherein the samples are obtained at different points in time, and (b) using the measurement value obtained to evaluate the state of the subject. 8. A method according to claim 6 or 7, wherein the subject evaluated is in treatment. 9. A method according to claim 8, wherein the subject is in highly active anti-retroviral therapy (HAART). 10. An ELISA-kit for evaluating the physical state of a subject suffering from HIV infection comprising a) a plate comprising a number of wells, b) an immobilised capture agent capable of capturing one or more marker in the form of (i) urokinase plasminogen activator receptor (uPAR), and/or (ii) soluble urokinase plasminogen activator receptor (suPAR), and/or one or more degradation products of (i) or (ii) and c) a binding partner capable of binding to the said marker, the binding partner comprising d) a label system. 11. An ELISA-kit for evaluating the physical state of a subject suffering from HIV infection comprising a) a plate comprising a number of wells, b) an immobilised capture agent capable of capturing a marker in the form of (i) urokinase plasminogen activator receptor (uPAR), and/or (ii) soluble urokinase plasminogen activator receptor (suPAR), and/or one or more degradation products of (i) or (ii) and c) a binding partner capable of binding to the said marker, the binding partner comprising d) a label system. 12. A stick-kit for evaluating the physical state of a subject suffering from HIV infection comprising a) a stick adapted to be contacted with a biological fluid sample and comprising means for displaying visually the result of the evaluation, b) an immobilised capture agent capable of capturing one or more markers in the form of (i) urokinase plasminogen activator receptor (uPAR), and/or (ii) soluble urokinase plasminogen activator receptor (suPAR), and/or more degradation products of (i) or (ii) and c) a binding partner capable of binding to the said marker, the binding partner comprising d) a label system. 13. A stick-kit for evaluating the physical state of a subject suffering from HIV infection comprising a) a stick adapted to be contacted with a biological fluid sample and comprising means for displaying visually the result of the evaluation, b) an immobilised capture agent capable of capturing a marker in the form of (i) urokinase plasminogen activator receptor (uPAR), and/or (ii) soluble urokinase plasminogen activator receptor (suPAR), and/or more degradation products of (i) or (ii) and c) a binding partner capable of binding to the said marker, the binding partner comprising d) a label system. 14. A kit according to claim 12 or 13, wherein the capture agent is an antibody to the marker. 15. A kit according to any one of claims 12 to 14, wherein the binding partner is an antibody to the marker.