Patent SG-11201901483Q-A (SG)
Title: Vaccine constructs and uses thereof against staphylococcus infections
Inventor: MALOUIN FRANÇOIS
IPC: C12N15/63
Filed: 2017-10-20
Abstract: - co 0 0 V ▪ ) C73 CO w O I- dp • • • n • • T • • • 00 bt56 n • o n - -00- - 1 638 400 409 600 102 400 n 25 600 6 400 1 600 400 00 00 Detection limit n Immune o Preimmune (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 April 2018 (26.04.2018) WIPO I PCT ill~~~~~~~~ 011101010VIIIOH olo olomiolonommom oimIE (10) International Publication Number WO 2018/072031 Al (51) International Patent Classification: C07K 14/31 (2006.01) Cl 2N 1/21 (2006.01) A61K 39/085 (2006.01) Cl 2N 15/31 (2006.01) A61P 31/04 (2006.01) Cl 2N 15/62 (2006.01) A61P 37/04 (2006.01) Cl 2N 15/63 (2006.01) CO7K 19/00 (2006.01) (21) International Application Number: PCT/CA2017/051253 (22) International Filing Date: 20 October 2017 (20.10.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/411,120 21 October 2016 (21.10.2016) US (71) Applicant: SOCPRA SCIENCES ET GENIES S.E.C. [CA/CA]; Pavillon Irene-Pinard, B6-3012, 2500, boul. de l'Universite, Sherbrooke, Quebec J1K 2R1 (CA). (72) Inventors: MALOUIN, Francois; 235 chemin d'Orford- sur-le-Lac, Eastman, Quebec JOE 1P0 (CA). STER, Ce- line; 142 Chemin de Cookshire, Compton, Quebec JOB 1L0 (CA). COTE-GRAVEL, Julie; 1091, rue Fairmount, app. #2, Sherbrooke, Quebec J1H 3N6 (CA). BROUILLETTE, Eric; 1143, rue de Forillon, Sherbrooke, Quebec J1N 4K9 (CA). (74) Agent: GOUDREAU GAGE DUBUC; 2000, avenue McGill College, #2200, Montreal, Quebec H3A 3H3 (CA). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (54) Title: VACCINE CONSTRUCTS AND USES THEREOF AGAINST STAPHYLOCOCCUS INFECTIONS NS O N O 00 O C Protein Mix A720AhemB Combination FIG. 16 (57) : There is provided a fusion construct of formula (I): X-A-linker-B-Z (I) wherein : (1) A and B are identical or different and are independently: (a) a polypeptide comprising a SACOL0029 polypeptide as set forth in any one of the sequences depicted in FIG. 24 (SEQ ID NOs: 5 and 121 to 131), a SACOL0264 polypeptide (SEQ ID NO : 185), a SACOL0442 polypeptide as set forth in any one of the sequences depicted in FIG. 22D (SEQ ID NOs: 29 and 82 to 92), a SACOL0718 polypeptide (SEQ ID NO : 186), a SACOL0720 polypeptide as set forth in any one of the sequences depicted in FIGs. 23I-J (SEQ ID NOs: 11 and 109 to 120), a SACOL1353 polypeptide (SEQ ID NO : 187), a SACOL1416 polypeptide (SEQ ID NO : 188), a SACOL1611 polypeptide (SEQ ID NO : 189), a SACOL1867 polypeptide as set forth in any one of the sequences depicted in FIG. 25D (SEQ ID NOs: 152 to 164), a SACOL1912 polypeptide (SEQ ID NO : 43), a SACOL1944 polypeptide (SEQ ID NO : 190), a SACOL2144 polypeptide (SEQ ID NO : 191), a SACOL2365 polypeptide (SEQ ID NO : 192), a SACOL2385 polypeptide (SEQ ID NO : 50) or a SACOL2599 polypeptide (SEQ ID NO : 193), based on the gene nomenclature from the Staphylococcus aureus COL (SACOL) genome set forth in [Continued on next page] WO 2018/072031 Al MIDEDIMOMOIDEIREEMONOMMIDIOMMEHOIS DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) NCBI Reference Sequence NC 002951.2; (b) a polypeptide encoded by a gene from a same operon as a gene encoding the polypeptide of (a); (c) a polypeptide comprising an immunogenic fragment of at least 13 consecutive amino acids of (a) or (b); (d) a polypeptide comprising an amino acid sequence at least 60% identical overall to the sequence of the polypeptide of any one of (a) to (c); or (e) a polypeptide comprising an immunogenic variant comprising at least 13 consecutive amino acids of any one of (a) to (c); (2) the linker is an amino acid sequence of at least one amino acid or is absent; (3) X is absent or is an amino acid sequence of at least one amino acid; and (4) Z is absent or is an amino acid sequence of at least one amino acid. Also provided are compositions and kits comprising the fusion and uses of these fusions, compositions and kits.