Patent SG-11201407806Y-A (SG)
Title: Mixed multifunctional metal affinity surfaces for reducing aggregate content in protein preparations field
Inventor: GAGNON PETER
IPC: C07K1/18
Filed: 2013-02-06
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 5 December 2013 (05.12.2013) WIPOIPCT (10) International Publication Number WO 2013/180649 A1 (51) International Patent Classification: C07K1/18 (2006.01) C07C 259/06 (2006.01) C07C 233/47 (2006.01) K01 1) 15/36 (2006.01) C07C 55/22 (2006.01) K01 1) 15/38 (2006.01) C07C 55/02 (2006.01) K01 1) 39/00 (2006.01) C07C 211/14 ( 2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000048 6 February 2013 (06.02.2013) English (30) Priority Data: 61/653,716 31 May 2012 (31.05.2012) English US (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis, Singapore 138632 (SG). (72) Inventor: GAGNON, Peter; 20 Biopolis Way, #06-01 Centres, Singapore 138668 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road, Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) 0\ •t o 00 i-H en i-H o CJ o & (54) Title: MIXED MULTIFUNCTIONAL METAL AFFINITY SURFACES FOR REDUCING AGGREGATE CONTENT IN PROTEIN PREPARATIONS FIELD (57) Abstract: Compositions for reducing the aggregate content of a protein preparation include a first substrate having a first sur­ face-bound ligand possessing metal affinity functionality a and a second surface-bound ligand optionally provided on a second sub - strate and having an aggregate charge opposite to that of the metal affinity functionality of the first substrate, wherein the first sur - face-bound ligand and the second surface-bound ligand are positioned such that the protein preparation may contact both the first surface-bound ligand and the second surface-bound ligand simultaneously.