Patent DE-102011104143-A1 (DE)
Title: Biochip based on silicon base produced in photolithography and etching technology useful in biological and medical analysis, comprises several individual recesses deposited in top e.g. silicon- or metal oxide layer of silicon based chips
IPC: C12M1/34
Filed: 2011-06-14
Abstract: Biochip based on silicon base produced in photolithography and etching technology, comprises several individual recesses deposited in the top e.g. silicon-, silicon dioxide- or metal oxide layer of the silicon based chips. The recesses serve as nano-reaction chambers for biochemical reactions, preferably the antigen-antibody-reaction for the purpose of analysis. The electronic connections of the chip are carried out such that conductivity variations in each of the reaction chambers are measured during the biological or biochemical detection-reaction and are transmitted to a computer. Biochip based on silicon base produced in photolithography and etching technology, comprises several individual recesses deposited in the top e.g. silicon-, silicon dioxide- or metal oxide layer of the silicon based chips. The recesses serve as nano-reaction chambers for biochemical reactions, where the biological and biochemical reactions take place in aqueous solution, preferably the antigen-antibody-reaction for the purpose of analysis. The electronic connections of the chip are carried out such that conductivity variations in each of the reaction chambers are measured during the biological or biochemical detection-reaction and are transmitted to a computer. Independent claims are also included for: (1) an apparatus comprising the biochip that is mounted movably in the apparatus, and immersed, introduced or moved through different reagent solutions, analyte solutions, serum, plasma, washing solutions, substrate solutions, incubators and/or air or gas blowers e.g. hair dryer. A magnet or coil acting as an electromagnet is mounted on the other side of the biochip so that when using magnetic beads in the reaction chambers, the magnet holds the beads during a movement of the biochip or during processing of the biochip with washer and reagent solutions in their respective reaction chambers. Different reagent- and washing solutions are applied (e.g. pipetted) on the biochip; (2) an apparatus for antigen-antibody based analysis, where further an enzyme-linked immunosorbent assay is performed in the reaction chamber, which is tracked and detected by measuring the conductivity i.e. of the analyte. A secondary antibody with bound enzyme on the respective primary antibody introduced into the respective reaction chamber also sits on the respective bead after at least one washing step, which converts a substrate. The detection is performed, e.g. by measuring the conductivity change during the substrate conversion by the enzyme. The binding of analyte on the bead loaded with primary antibody and the binding of the secondary antibody also takes place outside the reaction chamber. Similarly antigen-loaded beads are used to detect antibody; and (3) an apparatus for the antibody-based analysis, where further the conductivity in the individual reaction chamber is measured not only during the analyte binding but also during downstream phases of analysis, during downstream of the washing phases, during the binding of secondary antibody, an enzyme-linked secondary antibody or a modified or unmodified secondary antibody, during a downstream analytical detection reaction that occurs linked to or caused by the secondary antibody, or during the enzymatic conversion of substrate in the case of enzyme-linked immunosorbent assay.