Patent SG-11201901651T-A (SG)
Title: Compositions containing fusion protein of albumin and analogs thereof, methods for making and using the same
Inventor: CHU CHUN KWONG
IPC: C12N15/62
Filed: 2017-06-27
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111111111111111111111111111111111111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/038803 Al 01 March 2018 (01.03.2018) W I P0 I P C T (51) International Patent Classification: TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, A61K 38/31 (2006.01) C07K 14/765 (2006.01) EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, A61K 38/38 (2006.01) C07K 14/655 (2006.01) MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, A61K 38/22 (2006.01) Cl 2N 15/62 (2006.01) TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, A61K 38/16 (2006.01) A61P 5/02 (2006.01) KM, ML, MR, NE, SN, TD, TG). (21) International Application Number: PCT/US2017/039477 Published: — with international search report (Art. 21(3)) (22) International Filing Date: — before the expiration of the time limit for amending the 27 June 2017 (27.06.2017) claims and to be republished in the event of receipt of (25) Filing Language: English amendments (Rule 48.2(h)) — with sequence listing part of description (Rule 5.2(a)) (26) Publication Language: English (30) Priority Data: 15/249,346 26 August 2016 (26.08.2016) US (71) Applicant: NAL PHARMACEUTICAL GROUP LIMITED [CN/CN]; Rm 2109 Windsor House, 311 Gloucester Road, Causeway Bay, Hong Kong (CN). (72) Inventor; and (71) Applicant: MO, Joseph, Y. [US/US]; 1 Belleview Terrace, Princeton, New Jersey 08540 (US). Inventor: CHU, Chun Kwong; Room 1531, Herring Gull — (72) House, Shakok Estate, Shatin, NT, Hong Kong (CN). Agent: CHOE, Yun H.; Lucas & Mercanti, LLP, 30 Broad = (74) Street, 21st Floor, New York, New York 10004 (US). Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (81) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (84) ,_ 1 (54) Title: COMPOSITIONS CONTAINING FUSION PROTEIN OF ALBUMIN AND ANALOGS THEREOF, METHODS FOR MAKING AND USING THE SAME M (57) : The invention is related to fusion proteins of human somatostatin (e.g., SST-14 or SST-28) and human serum albumin, of comprising a region at least 85% homologous to human somatostatin and a region at least 85% homologous to human serum albumin pc or a region with a partial amino acid sequence of human serum albumin, wherein linker peptide sequences may be present between M somatostatin and somatostatin moieties or somatostatin and albumin moieties. Also disclosed are constructs wherein the somatostatin 0 --.... moiety contains multiple tandem repeats of a somatostatin sequence. In selected embodiments, the orientation of the somatostatin and Ot albumin moieties can be varied, and such sequences may impact the binding and efficacy of the disclosed fusion proteins. Also disclosed 1-1 C are methods of making and using the aforementioned constructs. The somatostatin-albumin fusion protein demonstrated enhanced N stability when incubated in rat plasma in vitro and prolonged plasma half-life in vivo compared with free somatostatin. C