Patent SG-11201407297V-A (SG)
Title: Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
Inventor: CHEN HAIBIN
IPC: C07K14/435
Filed: 2013-05-09
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau „ (10) International Publication Number (43) International Publication Date ^ ^ WO 2013/170050 A1 14 November 2013 (14.11.2013) WIPO I PCT (51) International Patent Classification: C07K14/435 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/040377 9 May 2013 (09.05.2013) English English (30) Priority Data: 61/646,100 11 May 2012 (11.05.2012) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Drive, Redwood City, California 94063 (US). (72) Inventors: CHEN, Haibin; Blk 716 Clementi W„ St 2 #06-11, Singapore 120716 (SG). MOORE, Jeffrey; 367 Orenda Circle, Westfield, New Jersey 07090 (US). COL­ LIER, Steven J.; 22 Burroughs Rd, Lexington, Massachu­ setts 02420 (US). SMITH, Derek; 12 Holland Avenue #10-31 272012, Singapore, Singapore 117525 (SG). NAZOR, Jovana; 200 Penobscot Dr, Redwood City, Cali­ fornia 94063 (US). HUGHES, Gregory; 2001 Hill Top Rd., Scotch Plains, New Jersey 07076 (US). JANEY, Jac­ ob; 50 West 72nd St Apt 1505, New York, New York 10023 (US). HUISMAN, Gjalt; 200 Penobscot Dr, Red­ wood City, California 94063 (US). NOVICK, Scott; 200 Penobscot Dr, Redwood City, California 94063 (US). AGARD, Nicholas; 200 Penobscot Dr, Redwood City, California 94063 (US). ALVIZO, Oscar; 200 Penobscot Dr, Redwood City, California 94063 (US). COPE, Gregory; 200 Penobscot Dr, Redwood City, California — 94063 (US). YEO, Wan Lin; Blk 213 Bedok North Street 1 #11-145, Singapore 460213 (SG). SUKUMARAN, Joly; Blk 703 W. Coast Rd. #11-391, Singapore 120703 (SG). NG, Stephanie; 200 Penobscot Dr, Redwood City, Cali­ fornia 94063 (US). (74) Agents: WHITING, Adam, K. et al.; CODEXIS, INC., 200 Penobscot Drive, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) © © o l> ^ (54) Title: ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND i-H AMINE COMPOUNDS o (57) Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding ^ the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engin- eered polypeptides with range of ketone a and amine substrate compounds to prepare secondary and tertiary amine product com- pounds.