Patent SG-11201810331Y-A (SG)
Title: Single chain variable fragment cd3 binding proteins
Inventor: DUBRIDGE ROBERT B
IPC: C07K16/28
Filed: 2017-05-19
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111111111111111111011111 110 11E111110 11111110 11111111111110 11111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\"1 WO 2017/201493 Al 23 November 2017 (23.11.2017) WIPO I PCT (51) International Patent Classification: (74) Agent: LIN, Clark Y.; WILSON SONSINI GOODRICH A61K 39/395 (2006.01) C07K 16/18 (2006.01) & ROSATI, 650 Page Mill Road, Palo Alto, California A61K 47/48 (2006.01) C07K 16/28 (2006.01) 94304 (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2017/033673 kind of national protection available): AE, AG, AL, AM, (22) International Filing Date: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 19 May 2017 (19.05.2017) CA, DZ, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (25) Filing Language: English HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, (26) Publication Language: English KW, MK, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, (30) Priority Data: PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, 62/339,685 20 May 2016 (20.05.2016) US SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicant: HARPOON THERAPEUTICS, INC. [US/US]; 4000 Shoreline Court, Suite 250, South San Fran- (84) Designated States (unless otherwise indicated, for every cisco, California 94080 (US). kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (72) Inventors: DUBRIDGE, Robert B.; 825 Holly Road, Bel- UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, mont, California 94002 (US). SETO, Pui; 292 Clifton Ave., TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, San Carlos, California 94070 (US). AUSTIN, Richard J.; EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, 1169 Guerrero St., San Francisco, California 94110 (US). MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, EVNIN, Luke; 768 El Camino Del Mar, San Francisco, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, = California 94121 (US). GUENOT, Jeanmarie; 45 Juniper KM, ML, MR, NE, SN, TD, TG). Street, #3, San Francisco, California 94103 (US). LEMON, Bryan D.; 2493 Dell Ave., Mountain View, California — 94043 (US). = (54) Title: SINGLE CHAIN VARIABLE FRAGMENT CD3 BINDING PROTEINS = = = = 4e-.13 = = 3e , 6 - _ = = V c = 0 2e-6 - • bCD3 ---0-- VVI3 = CO — v— bHSA Molar Eq = a) —6-- bHSA Mass Eq _ = ra Ie.-6 - = 0 _ 1-1 1 1 1 1 0. 0 2 0e+10 4 Oe+10 6 Oe+10 8.0e+10 1 1 Oe+11 1.2e+11 M t:t 71- Phage Plated FIG. 1 1-1 0 N ---- (57) : Disclosed herein are single chain variable fragment CD3 binding proteins with improved binding affinities, and robust IN ,- 1 aggregation profiles. Also described are multispecific binding proteins comprising a single chain variable fragment CD3 binding protein 0 according to the instant disclosure. Pharmaceutical compositions comprising the binding proteins disclosed herein and methods of using N such formulations are provided. C [Continued on next page] WO 2017/201493 Al MIDEDIM111101111111111111111111111111111111111111110111111111111111111111111111111111 Declarations under Rule 4.17: — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(H)) — as to the applicant's entitlement to claim the priority of earlier application (Rule 4.17(iii)) the Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt amendments (Rule 48.2(h)) of — with sequence listing part of description (Rule 5.2(a))