Patent DE-102009028493-A1 (DE)
Title: Microfluidic cell e.g. flow cell for dielectrophoretic separation, accumulation and/or lysis of polarizable bioparticles e.g. bacteria, includes interdigital electrode system made of electrode groups with interdigitally disposed electrodes
Inventor: STUMBER MICHAEL
IPC: B01L3/00
Filed: 2009-08-13
Abstract: The microfluidic cell such as flow cell for dielectrophoretic separation, accumulation and/or lysis of polarizable bioparticles such as bacteria, cells and/or viruses, comprises an interdigital electrode system made of two electrode groups with interdigitally disposed electrodes (1a, 1b), and a micromixer having microchannels (2a) and micro-projections (2b). The interdigital electrode system and the micromixer are arranged on the same side of the microfluidic cell. The electrodes are arranged in the microchannels and form a combined interdigital-micromixer-system. The microfluidic cell such as flow cell for dielectrophoretic separation, accumulation and/or lysis of polarizable bioparticles such as bacteria, cells and/or viruses, comprises an interdigital electrode system made of two electrode groups with interdigitally disposed electrodes (1a, 1b), and a micromixer having microchannels (2a) and micro-projections (2b). The interdigital electrode system and the micromixer are arranged on the same side of the microfluidic cell. The electrodes are arranged in the microchannels and form a combined interdigital-micromixer-system. The electrodes and/or the microchannels and/or micro-projections are formed and arranged parallel to each other with respect to the flow direction in an angle of 20-70[deg] , and formed and arranged in a zig-zag manner or in the form of parallel slash pattern. The micromixer is made of an insulating material. The microfluidic cell has flat electrode, which is arranged at one side of the microfluidic cell that faces the side, at which the interdigital electrode system and the micromixer are arranged. The flat electrode corresponds to the surface of the combined interdigital-micromixer-system. The microfluidic cell includes a further interdigital electrode system made of two electrode groups with interdigitally disposed electrodes, and a further micromixer having microchannels and micro-projections. The combined interdigital-micromixer-system has a length of 10-60 mm, a width of 3-30 mm and/or an area (Lx B) of 30-1800 mm 2>. The microchannels and/or micro-projections have a length of 3-30 mm. The distance between the two microchannels is 30-500 mu m. The microchannels have a width of 30-800 mu m. The micro-projections have a width of 30-500 mu m and a height of 10-400 mu m. The microchannels have a depth of 10-400 mu m. The electrodes of the interdigital electrode system have a length of 3-30 mm, a width of 10-500 mu m, a height of 0.1-50 mu m and/or a distance of 10-500 mu m. The microfluidic cell has a length, a width of 3-40 mm and/or a height of 20-1000 mu m. The flat electrode has a length of 10-100 mm, a width of 3-50 mm, a height of 0.1-50 mu m and/or an area (Lx B) of 30-1800 mm 2>. An independent claim is included for a method for separation and/or accumulation of polarizable bioparticles with a microfluidic cell.