Patent SG-11201806018Y-A (SG)
Title: Protein cage-stabilized pickering emulsions and the use thereof
Inventor: LIM SIERIN
IPC: A61K47/42
Filed: 2017-01-16
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization M1111101110101011111 HO 11111 011101110111011011110011011111011 III 11 International Bureau 0.. ..... ..Yi jd (10) International Publication Number ...... ......:;,/ (43) International Publication Date WO 2017/123160 Al 20 July 2017 (20.07.2017) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A61K 8/06 (2006.01) A61K 47/42 (2017.01) kind of national protection available): AE, AG, AL, AM, A61K 9/107 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/SG2017/050020 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (22) International Filing Date: KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, 16 January 2017 (16.01.2017) MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (25) Filing Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (26) Publication Language: English TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 10201600290W 14 January 2016 (14.01.2016) SG (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicants: NANYANG TECHNOLOGICAL UNI- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, VERSITY [SG/SG]; 50 Nanyang Avenue, Singapore TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, 639798 (SG). AGENCY FOR SCIENCE, TECHNO- TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, LOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, #20-10, Connexis, Singapore 138632 (SG). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, (72) Inventors: LIM, Sierin; c/o Nanyang Technological Uni- — versity, 50 Nanyang Avenue, Singapore 639798 (SG). GW, KM, ML, MR, NE, SN, TD, TG). — SARKER, Miridul; c/o Nanyang _ Technological Univer- Published: = sity, 50 Nanyang Avenue, Singapore 639798 (SG). TOM- — with international search report (Art. 21(3)) for Science, Technology Way, #20-10, Connexis, — with sequence listing part of description (Rule 5.2(a)) CZAK, Nikodem; c/o Agency = And Research, 1 Fusionopolis Singapore 138632 (SG). = (74) Agent: VIERING, JENTSCHURA & PARTNER LLP; Office, Rochor Road, Singa- . P.O Box 1088, Rochor Post pore 911833 (SG). PICKERING EMULSIONS AND THE USE THEREOF (54) Title: PROTEIN CAGE-STABILIZED 11 = — _ Figure = 1 = nm = 25 = ,.,b., . structure of E2 Protein cage Aqueou Crystallograhic 1-1 *1! with loaded cargo Phase 0 it Hydrophobic Moiecule Schematic diagram of E2 protein Hydrophilic Molecule cage-stabilized Pickering emulsion 1-1 cr) r‘l 1-1 It-- ._, (57) : The present invention relates to a Pickering emulsion comprising an aqueous phase, an oil phase and a nanoparticle, ::::; wherein the nanoparticle is a protein cage. The protein cage is preferably a Bacillus stearothermophilus E2 protein of pyruvate de- e , ' hydrogenase multi-enzyme complex or an E2LC2 protein. Preferably the aqueous or oil phase of the Pickering emulsion com prises 0 an agent, or the protein cage is coupled to or loaded with an agent, wherein the agent is a therapeutic, nutritional, nutraceutical or cosmetic agent. The invention further includes use of the Pickering emulsions disclosed herein in pharmaceutical, cosmetic, or food applications, or as a controlled release delivery system, and use of protein cages as emulsifiers in Pickering emulsions.