Patent SG-11201808698T-A (SG)
Title: Fixed-bed bioreactor with constant-flow pump/ tubing system
Inventor: LESCH HANNA
IPC: C12M1/00
Filed: 2017-04-03
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11M1111101110101011111 HO 11111 01110111101111110011111011111110fi0I II International Bureau ... .... ..Yjd ..... ...,/ (10) International Publication Number (43) International Publication Date WO 2017/180341 A2 19 October 2017(19.10.2017) WIPO I PCT (51) International Patent Classification: Heinaahontie 9, 70870 Hiltulanlahti (FI). TUUNANEN, C12P 21/02 (2006.01) C12M 1/02 (2006.01) Tarja, Hannele; Maitotie 7 C 19, 70780 Kuopio (FI). (21) International Application Number: KARNAATTU, Milla Jonna, Karoliina; Lehvakuja 6, PCT/US2017/025681 70150 Kuopio (FI). MULLER, Achim; Susiniementie 238, 71150 Vartiala (FI). KARHINEN, Minna, Kristiina; (22) International Filing Date: Sammonkatu 28 A16, 70500 Kuopio (FI). 3 April 2017 (03.04.2017) (74) Agent: POHL, Mark; Pharmaceutical Patent Attorneys, (25) Filing Language: English LLC, 55 Madison Avenue, 4th Floor, Morristown, NJ 07960-7397 (US). (26) Publication Language: English (81) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of national protection available): AE, AG, AL, AM, 62/322,651 14 April 2016 (14.04.2016) US AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (71) Applicant: TRIZELL LTD. [GB/GB]; Sanderum House BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, Oakley Road, Chinnor, Oxfordshire OX39 4TW (GB). DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (71) Applicant (for US only): PHARMACEUTICAL PAT- KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, ENT ATTORNEYS, LLC [US/US]; 55 Madison Avenue, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, 4th Floor, Morristown, NJ 07960 (US). NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (72) Inventors: LESCH, Hanna, P.; Microkatu 1S, 70210 RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, Kuopio (Fl). VALONEN, Piia, Kristiina; Maaherrankatu — ZA, ZM, ZW. 1 B 38, 70100 Kuopio (FI). RASANEN, Eva, Kristiina; [Continued on next page] (54) Title: FIXED-BED _ — _ — - = = = = . -it— BIOREACTOR WITH CONSTANT-FLOW PUMP/ TUBING SYSTEM (57) : We have modified a commercially-available adherent cell culture bioreactor in several ways to increase productivity of cultured cells, while decreasing contamination risk. We found that modifying a commer- cially-available adherent cell culture bioreactor to provide for slower cell culture medium flow unexpectedly and dramatically increases the productiv- B ity of the cultured adherent cells. We also developed a new sampling mani- fold configuration and new way of taking samples, to reduce contamination risk. = = _ W se a ---- r = ei 2 — Il 7r M © GC O W I , 0 0 :00 0 0 III /a FIG. 2 ,-1 IN ,-1 © ei O WO 2017/180341 A2 IMIOHOIIIThigThil1130H011111VOIRMON (84) Designated States (unless otherwise indicated, for every as to applicant's entitlement to apply for be granted and kind of regional protection available): ARIPO (BW, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, GH, SZ, RU, — DE, LT, SE, a patent (Rule 4.17(ii)) as to the applicant's entitlement to claim the earlier application (Rule 4.17(iii)) of inventorship (Rule 4.17(iv)) the priority of SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, Published: GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — without international search report and to upon receipt of that report (Rule 48.2(g)) be republished — as to the identity of the inventor (Rule 4.17(i))