Patent SG-11201807124P-A (SG)
Title: Surface enhanced raman spectroscopy (sers) microfluidics biosensor for detecting single and/or multiple analytes
Inventor: OLIVO MALINI
IPC: G01N33/50
Filed: 2017-03-23
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization MIN1011110111010101H HO 111110111011110011111HOIHOIMIOU III International Bureau no .... ..Y1 j (10) International Publication Number (43) International Publication Date ... ......7:2 WO 2017/164815 Al 28 September 2017 (28.09.2017) WIP0 I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every GO1N 21/65 (2006.01) G01N 33/50 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/SG2017/050144 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, 23 March 2017 (23.03.2017) KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (25) Filing Language: English NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (26) Publication Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (30) Priority Data: ZA, ZM, ZW. 10201602281Y 23 March 2016 (23.03.2016) SG (84) Designated States (unless otherwise indicated, for every (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY kind of regional protection available): ARIPO (BW, GH, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Connexis North Tower, Singapore 138632 (SG). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (72) Inventors: OLIVO, Malini; c/o Singapore Bioimaging TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, Consortium, 11 Biopolis Way, Helios #01-02, Singapore DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, 138667 (SG). PERUMAL, Jayakumar; c/o Singapore SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Bioimaging Consortium, 11 Biopolis Way, Helios #01-02, _ GW, KM, ML, MR, NE, SN, TD, TG). Singapore 138667 (SG). BALASUNDARAM, Ghayathri; — _ c/o Singapore Bioimaging Consortium, 11 Biopolis Way, Published: Helios #01-02, Singapore 138667 (SG). — — with international search report (Art. 21(3)) Agent: VIERING, JENTSCHURA & PARTNER LLP; before the expiration of the time limit for amending the (74) P.O. Box 1088, Rochor Post Office, Rochor Road, Singa- claims and to be republished in the event of receipt of pore 911833 (SG). amendments (Rule 48.2(h)) = Title: SURFACE ENHANCED RAMAN SPECTROSCOPY (SERS) MICROFLUIDICS BIOSENSOR FOR DETECTING (54) AND/OR MULTIPLE ANALYTES SINGLE = FIG. 10 (57) : A biosensor for the detection of an analyte using surface-enhanced Raman spectroscopy (SERS) is provided. The bio- sensor includes a SERS-active substrate and a microfludic circuit device arranged to be in = = MI]ro'luidic fluid communication with the SERS-active substrate. Method of manufacturing a bio - sensor, and methods for detecting an analyte using the biosensor, wherein the analyte may = = = Device Ai 1 , 1ZrotIiid[u clitp be haptoglobin, are also provided. S :kb substrate Conjugate different = — 1°Antibody in each channel Incubation with Blofluid /protein mixture & washing .,4 — 1% BSA treatment & Il 2° washing lb Antibody t ,;nobody I Conjugate Antibody with SERS Biomarker 2 Bi °marker I ir) 1 tag mixture Il GC Incubation & 71' 4= 0 SERS` : 1-1 - 1 , 7 T -7fr r- 4 .+Y. measurement IN 1-1 0 N O