Patent SG-11201805124V-A (SG)
Title: Methods and compositions of insect control
Inventor: ARHANCET JUAN PEDRO
IPC: C07H21/04
Filed: 2016-12-07
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111010111110111011101111101100111011111111101111011111 International Bureau ... .... ..Yjd ..... .....!;,,, (10) International Publication Number (43) International Publication Date WO 2017/116644 Al 6 July 2017 (06.07.2017) WI P0 I P CT (51) International Patent Classification: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, C12N 1/20 (2006.01) Cl 2N 15/00 (2006.01) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, C12N 1/00 (2006.01) CO7H 21/04 (2006.01) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (21) International Application Number: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, PCT/US2016/065408 TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (22) International Filing Date: ZW. 7 December 2016 (07.12.2016) (84) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (26) Publication Language: English TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (30) Priority Data: TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, 62/273,654 31 December 2015 (31.12.2015) US DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (71) Applicant: APSE, INC. [US/US]; 4320 Forest Park Aven- SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, ue, Suite 303, St. Louis, Missouri 63018 (US). GW, KM, ML, MR, NE, SN, TD, TG). (72) Inventors: ARHANCET, Juan Pedro, Humberto; 4320 Declarations under Rule 4.17: Forest Park Avenue, Suite 303, St. Louis, Missouri 63108 — (US). KILLMER, John, L. 4320 Forest Park Avenue, as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(0) Suite 303, St. Louis, Missouri 63108 (US). McLAUGH- — LIN, Patrick, D.; 4320 Forest Park Avenue, Suite 303, St. — of inventorship (Rule 4.17(iv)) — Louis, Missouri 63108 (US). Published: (74) Agents: CAMPBELL, John, W. et al.; Morgan, Lewis & international 21(3)) _ with search report (Art. = Bockius LLP, Attention: Docketing Department, 1111 Pennsylvania Avenue, NM., DC 20004 — before the expiration of the time limit for amending the Washington, (US). _ claims and to be republished in the event of receipt of (81) Designated States (unless otherwise indicated, for every amendments (Rule 48.2(h)) = kind of national protection available): AE, AG, AL, AM, = AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, with sequence listing part of description (Rule 5.2(a)) . BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, = = = = = = = Il 7r 7r ,tD ,tD 1-1 11 (54) Title: METHODS AND COMPOSITIONS OF INSECT CONTROL I N (57) : The invention describes recombinant DNA sequences transcribed into RNA constructs capable of forming Virus Like C Particles (VLPs) suitable for insect control applications. Specifically, the disclosure provides a method for controlling target insects ei comprising, transforming a microbial host with a first DNA sequence comprising a gene encoding a bacteriophage capsid protein en. ) and a second DNA sequence encoding an RNA transcript comprising at least one bacteriophage pac sequence coupled to an RNAi precursor sequence, inducing the microbial host to express the first and second DNA sequences, isolating virus-like-particles (VLPs) comprising the capsid protein and RNAi precursor from the microbial host, and contacting the isolated VLPs with the target insects.