Patent SG-11201407789R-A (SG)
Title: Humanized non-human animals with restricted immunoglobulin heavy chain loci
Inventor: MACDONALD LYNN
IPC: C07K16/40
Filed: 2013-03-07
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 19 December 2013 (19.12.2013) WIPOIPCT (10) International Publication Number WO 2013/187953 A1 (51) International Patent Classification: A01K 67/027 ( 2006.01) C07K16/40 (2006.01) C07K16/28 (2006.01) C07K16/46 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/US2013/029624 7 March 2013 (07.03.2013) English (30) Priority Data: 61/658,466 61/663,131 12 June 2012 (12.06.2012) 22 June 2012 (22.06.2012) English US US (71) Applicant: REGENERON PHARMACEUTICALS, INC. [US/US]; 777 Old Saw Mill River Road, Tarrytown, NY 10591 (US). (72) Inventors: MACDONALD, Lynn; 16 Gedney Way, White Plains, NY 10605 (US). MCWHIRTHER, John; 2152 Crescent Drive, Tarrytown, NY 10591 (US). TU, Naxin; 514 Washington Avenue, Pleasantville, NY 10570 (US). STEVENS, Sean; 355 Berry Street #413, San Fran­ cisco, CA 94158 (US). MURPHY, Andrew, J.; 10 New­ ton Court, Croton-on-Hudson, NY 10520 (US). (74) Agent: POBURSKY, Kevin J.; Choate, Hall & Stewart LLP, Two International Place, Boston, MA 02110 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) o\ i> 00 i-H en i-H o CJ o & (54) Title: HUMANIZED NON-HUMAN ANIMALS WITH RESTRICTED IMMUNOGLOBULIN HEAVY CHAIN LOCI (57) Abstract: Mice, embryos, cells, and tissues having restricted immunoglobulin heavy chain a locus and an ectopic sequence en­ coding one or more ADAM6 proteins are provided, in various embodiments, mice are described that have humanized endogenous immunoglobulin heavy chain loci and are capable of expressing an ADAM6 protein or ortholog or homolog or functional fragment thereof that is functional in a male mouse. Mice, embryos, cells, and tissues having an immunoglobulin heavy chain locus character­ ized by a single human VH gene segment, a plurality of human D H gene segments and plurality a of human J H gene segments and cap­ able expressing an ADAM6 protein or ortholog or homolog or functional fragment thereof are also provided.