Patent SG-11201907008R-A (SG)
Title: Methods and microorganisms for making 2,3-butanediol and derivatives thereof from c1 carbons
Inventor: ZHAO XINHUA
IPC: C12N9/04
Filed: 2018-01-30
Abstract: AisSOH OH -acetolactate co o pyruvate OH 0 CO 2 OH BudA rt. tr% OH OH Acetoin NAD(P)H NAD(P) 2,3 BDO ButA CO 2 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 02 August 2018 (02.08.2018) WIPO I PCT ill Hu °ninon olo olo Ho 11°111°1°1110 iflo oimIE (10) International Publication Number WO 2018/140928 Al (51) International Patent Classification: C12N 1/00 (2006.01) Cl 2N 15/52 (2006.01) C12N 1/21 (2006.01) Cl 2N 15/63 (2006.01) C12N 9/00 (2006.01) Cl 2P 7/16 (2006.01) C12N 9/04 (2006.01) (21) International Application Number: PCT/US2018/015909 (22) International Filing Date: 30 January 2018 (30.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/451,819 30 January 2017 (30.01.2017) US 62/504,626 11 May 2017 (11.05.2017) US 62/512,312 30 May 2017 (30.05.2017) US 62/588,985 21 November 2017 (21.11.2017) US (71) Applicant: INTREXON CORPORATION [US/US]; 1750 Kraft Drive, Suite 1400, Blacksburg, Virginia 24060 (US). (72) Inventors: ZHAO, Xinhua; 2771 Alliston Loop, Dublin, California 94568 (US). HELD, Mark, Anton; 1030 45th Street, REAR, Emeryville, California 94608 (US). HUYNH, Tina; 3301 High Street, Oakland, California 94619 (US). CHAO, Lily, Yuin; 72 Langton Street, Unit 3, San Francisco, California 94103 (US). TRINH, Na; 1839 Ygnacio Valley Road #181, Walnut Creek, Califor- nia 94598 (US). SCHAMLISCH, Matthias, Helmut; 340 Holly Avenue, South San Francisco, California 94080 (US). YEH, Bryan; 156 Arlene Drive, Walnut Creek, Califor- nia 94595 (US). KEALEY, James; 945 Midpine Way, Se- bastopol, California 95472 (US). DIETZEL, Kevin, Lee; 963 Fassler Avenue, Pacifica, California 94044 (US). (74) Agent: KELLY, David, A. et al.; Hunton & Williams LLP, Intellectual Property Department, 2200 Pennsylvania Ave., NW, Washington, District of Columbia 20037 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to the identity of the inventor (Rule 4.17(i)) as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(ii)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) — (54) Title: METHODS AND MICROORGANISMS FOR MAKING 2,3-BUTANEDIOL AND DERIVATIVES THEREOF FROM Cl CARBONS Methane FIG.1 1-1 00 (57) : Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as O 2,3-BDO are disclosed. For example, genetically modified methanotrophs that are capable of generating 2,3-BDO at high titers from \" a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also C disclosed. [Continued on next page] WO 2018/140928 Al MIDEDIMOMMIDIREEMOMMEENHOIEHMEEN Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))