Patent SG-11201805592R-A (SG)
Title: Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
Inventor: CHAPMAN KEVIN T
IPC: C07K16/00
Filed: 2017-01-13
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization MD 1111101110101011111 HO 11111 0111011101110011110111 10111110H III International Bureau 0.. .... .. ..... ..or:;„, (10) International Publication Number (43) International Publication Date WO 2017/123978 Al 20 July 2017 (20.07.2017) WIPO I PCT (51) International Patent Classification: [CN/US]; 5858 Horton Street, Suite 320, Emeryville, Cali- C07K16/00 (2006.01) C07K 16/30 (2006.01) fornia 94608 (US). FOX, George L. [US/US]; 5858 Hor- C07K 16/28 (2006.01) G01N 33/569 (2006.01) ton Street, Suite 320, Emeryville, California 94608 (US). RADEL, Peggy A. [US/US]; 5858 Horton Street, Suite (21) International Application Number: 320, Emeryville, California 94608 (US). PCT/US2017/013483 (74) Agents: MCNEILL, Rebecca M. et al.; McNeill Baur (22) International Filing Date: PLLC, 125 Cambridge Park Drive, Suite 301, Cambridge, 13 January 2017 (13.01.2017) Massachusetts 02140 (US). (25) Filing Language: English (81) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (30) Priority Data: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, 62/279,341 15 January 2016 (15.01.2016) US DO, DZ, EC, EE, EG, ES, FE GB, GD, GE, GH, GM, GT, 62/411,690 23 October 2016 (23.10.2016) US HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, 62/412,092 24 October 2016 (24.10.2016) US KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, Horton Street, Suite 320, Emeryville, California 94608 NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (US). RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (72) Inventors; and ZA, ZM, ZW. (71) Applicants : CHAPMAN, Kevin T. [US/US]; 5858 Hor- - ton Street, Suite 320, Emeryville, California 94608 (US). (84) Designated States (unless otherwise indicated, for every _ kind ARIPO (BW, GH, WHITE, Mark P. [US/US]; 5858 Horton Street, Suite of regional protection available): GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 320, Emeryville, California 94608 (US). WANG, Xiaohua = 5858 Horton Street, Suite 320, Emeryville, Cali- TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, [CN/US]; fornia 94608 (US). PARK, Minha [US/US]; 5858 Horton TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FE FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, = Street, Suite 320, Emeryville, California 94608 (US). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, STADLER, Guido K. [AT/US]; 5858 Horton Street, Emeryville, California 94608 (US). LOWE, JR., Randall SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, = D. 5858 Horton Street, Suite 320, Emeryville, GW, KM, ML, MR, NE, SN, TD, TG). [US/US]; = California 94608 (US). GUAN, Xiao [CN/US]; 5858 Hor- Published: = ton Street, Suite 320, Emeryville, California 94608 (US). with international search report (Art. 21(3)) — MCEWEN, Jason M. [US/US]; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). WANG, Gang, F. = = = = = = = 1-1 GC IN C :N (54) Title: METHODS OF PRODUCING PATIENT-SPECIFIC ANTI-CANCER THERAPEUTICS AND METHODS OF TREAT- en el MENT THEREFOR 1-1 ---- (57) : A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from IN at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a C flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissoci - el ated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) us - , ing the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells ,71 . 9 can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engin - .,. eered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.