Patent SG-11201908337V-A (SG)
Title: Stimulatory cell lines for ex vivo expansion and activation of natural killer cells
Inventor: KAMIYA TAKAHIRO
IPC: A61P35/00
Filed: 2018-03-27
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 04 October 2018 (04.10.2018) WIP0 I PCT onion °noI0 OH1101OHHOH0II111111 0IIIIII (10) International Publication Number WO 2018/182511 Al (51) International Patent Classification: C12N 5/0783 (2010.01) Cl 2N 15/13 (2006.01) A61K 35/17 (2015.01) A61P 35/00 (2006.01) C12N 15/24 (2006.01) A61P 31/00 (2006.01) C12N 15/28 (2006.01) (21) International Application Number: PCT/SG2018/050138 (22) International Filing Date: 27 March 2018 (27.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/477,311 27 March 2017 (27.03.2017) US (71) Applicant: NATIONAL UNIVERSITY OF SINGA- PORE [SG/SG]; 21 Lower Kent Ridge Road, Singapore 119077 (SG). (72) Inventors: KAMIYA, Takahiro; c/o National University of Singapore, Yong Loo Lin School of Medicine, 21 Low- er Kent Ridge Road, Singapore 119077 (SG). CAMPANA, Dario; c/o National University of Singapore, Yong Loo Lin School of Medicine, 21 Lower Kent Ridge Road, Singapore 119077 (SG). (74) Agent: AMICA LAW LLC; 30 Raffles Place, #14-01 Chevron House, Singapore 048622 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) W O 20 18/ 18 25 11 Al (54) Title: STIMULATORY CELL LINES FOR EX VIVO EXPANSION AND ACTIVATION OF NATURAL KILLER CELLS k562-mb15- +mb12 +mb113 +mb12 41BBL +mbl3 (57) : The present invention relates to genetically engineered cell populations derived from an immortalised/ cancerous cell that do not express MHC class I molecules but that are modified to express membrane-bound IL-15, membrane-bound 4-1 BBL ligand, and at least one other membrane bound molecule, such as an interleukin or anti-CD3 antibody. The co-culture of said cells with a population of immune cells results in the activation and expansion of at least one subpopulation of immune cells. Expanded populations of NK cells derived from the co-culture of a mixed cell culture with the stimulatory cell lines may be used in methods of treating cancer or an infectious disease. In a separate embodiment, a plurality of nucleic acids for use in preparing the engineered cell population are provided. [Continued on next page] WO 2018/182511 Al IIMEDIM01101 DIDEFIE0ME101IIIIIIIM01111111111111111111111111 Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE