Patent SG-11201909470T-A (SG)
Title: Bi- or multi-differentiated organoid
Inventor: KNOBLICH JÜRGEN
IPC: C12N5/079
Filed: 2018-04-25
Abstract: PCTEP/060559 25 April 2018 (25.04.2018) English English (51) International Patent Classification: C12N 5/071 (2010.01) C12N 5/079 (2010.01) (21) International Application Number: (22) International Filing Date: Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (25) Filing Language: (26) Publication Language: (30) Priority Data: 17168051.5 25 April 2017 (25.04.2017) EP (71) Applicant: IMBA - INSTITUT FUR MOLEKULARE BIOTECHNOLOGIE GMBH [AT/AT]; Dr.Bohrgasse 3, 1030 Vienna (AT). (72) Inventors: KNOBLICH, Jurgen; c/o IMBA - INSTITUT FUR MOLEKULARE BIOTECHNOLOGIE GMBH, Dr. Bohrgasse 3, 1030 Vienna (AT). BAGLEY, Joshua A.; c/ o IMBA - INSTITUT FUR MOLEKULARE BIOTECH- NOLOGIE GMBH, Dr. Bohrgasse 3, 1030 Vienna (AT). (74) Agent: SONN & PARTNER PATENTANWALTE; Riemergasse 14, 1010 Vienna (AT). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). N 00 O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 November 2018 (01.11.2018) WIPO I PCT ill mu °mons °nolo Oil mo Ho oimIE (10) International Publication Number WO 2018/197544 Al (54) Title: BI- OR MULTI-DIFFERENTIATED ORGANOID (57) : The present invention relates to an in vitro method of producing a bi- or multi-differentiated tissue with at least two tissue types comprising the steps of developing a first tissue to a stage of differentiation of interest, developing a second tissue to a stage of differentiation of interest differing from the stage of differentiation of interest of the first tissue, placing said first and second tissue in a vicinity sufficient for fusion by growth, allowing the first and second tissue to grow and fuse to each other, thereby producing a bi- or multi- differentiated tissue comprising the first and second tissue with different stages of differentiation; bi- or multi- differentiated tissue obtained by such a method; uses of the tissue and kits for performing the method.