Patent SG-11201901221Y-A (SG)
Title: Methods for detecting aav
Inventor: JIN XIAOYING
IPC: G01N33/68
Filed: 2017-08-14
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 111111111111111111 11111111110111110100111111011100111111111111011111011110111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/035059 Al 22 February 2018 (22.02.2018) WIP0 I PCT (51) International Patent Classification: C12N 7/00 (2006.01) G01N 33/68 (2006.01) Published: C12N 15/86 (2006.01) — with international search report (Art 21(3)) — before the expiration of the time limit for amending the (21) International Application Number: claims and to be republished in the event of receipt of PCT/US2017/046814 amendments (Rule 48.2(h)) (22) International Filing Date: — with sequence listing part of description (Rule 5.2(a)) 14 August 2017 (14.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/375,314 15 August 2016 (15.08.2016) US (71) Applicant: GENZYME CORPORATION [US/US]; 500 Kendall Street, Cambridge, MA 02142 (US). (72) Inventors: JIN, Xiaoying; c/o Sanofi, 55 Corporate Drive, Bridgewater, NJ 08807 (US). O'RIORDAN, Catherine, R.; c/o Sanofi, 55 Corporate Drive, Bridgewater, NJ 08807 - (US). LIU, Lin; c/o Sanofi, 55 Corporate Drive, Bridgewa- ter, NJ 08807 (US). ZHANG, Kate; c/o Sanofi, 55 Corpo- - rate Drive, Bridgewater, NJ 08807 (US). = Agent: DONAHUE, Brian, A. et al.; Morrison & Foerster = (74) LLP, 755 Page Mill Road, Palo Alto, CA 94304-1018 (US). = Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (81) = = DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, = HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, = = SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = = Designated States (unless otherwise indicated, for every (84) = kind of regional protection available): ARIPO (BW, GH, = GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, = UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, = TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, — EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). = — 1-1 ..! Declarations under Rule 4.17: ON — of inventorship (Rule 4.17(iv)) 1 1 in © en 11 in (54) Title: METHODS FOR DETECTING AAV ---- (57) : Provided herein are methods for determining the serotype of a virus particle and/or or determining the heterogeneity GC , 1 of a virus particle (e.g., an AAV particle). In other embodiments, the invention provides methods to determine the heterogeneity of C::::) AAV particles. In some aspects, the invention provides viral particles (e.g., rAAV particles) with improved stability and/or improved N transduction efficiency by increasing the acetylation and/or deamidation of capsid proteins. C