Patent AU-2013204140-B2 (AU)
Title: Common light chain mouse
Inventor: BABB ROBERT
IPC: A01K67/027
Filed: 2013-03-05
Granted: 2016-01-28
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/134263 A1 12 September 2013 (12.09.2013) W I P 0 I P C T (51) International Patent Classification: (74) Agent: ZIPPIN, Margarita; Regeneron Pharmaceuticals, A01K67/027(2006.01) C07K16/00 (2006.01) Inc., 777 Old Saw Mill River Rd, Tarrytown, NY 10591 C12N15/85 (2006.01) (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2013/029125 kind of national protection available): AE, AG, AL, AM, .) . AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (22)I5 March 2013 (05.03.2013) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, (25) Filing Language: English HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, (26) Publication Language: English ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (30) Priority Data: NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, 13/412,936 6 March 2012 (06.03.2012) US RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, (71) Applicant: REGENERON PHARMACEUTICALS, ZM, ZW. INC. [US/US]; 777 Old Saw Mill River Road, Tarrytown, NY 10591 (US). (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (72) Inventors: BABB, Robert; 292 Voorhis Ave, River Edge, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, NJ 07661 (US). McWHIRTER, John; 2152 Crescent UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, Drive, Tarrytown, NY 10591 (US). MACDONALD, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Lynn; 16 Gedney Way, White Plains, NY 10505 (US). EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, STEVENS, Sean; 355 Berry Street #413, San Francisco, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, CA 94158 (US). DAVIS, Samuel; 332 West 88th Street, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Apt. B2, New York, NY 10024 (US). BUCKLER, David, ML, MR, NE, SN, TD, TG). R.; 6 Erick Court, Chester, NJ 07930 (US). MEAGHER, Published: Karolina, A.; 14 Church Street, 2nd Floor, Tarrytown, NY 10591 (US). MURPHY, Andrew, J.; 10 Newton Court, - with international search report (Art. 21(3)) Croton-on-hudson, NY 10520 (US). - with sequence listing part of description (Rule 5.2(a)) (54) Title: COMMON LIGHT CHAIN MOUSE Mouse - CK Deleted Endogenous Mouse lgw LocusA Enh Enh Mouse Mouse Rearranged Germline VK3-7 Leader CK Human VK'1-39JK5 FLE N~~eo Fr No 8I Targeting Vector Human Human Enh Enh -V.3-15 W1-39J.5 Promoter + FLP + 4F L M ouse M o use Vx3 7 Leader Cs Engineered Human ..k e *I Vi1-39Jx5 Locus Human Huan En Eh V3-15 V1-39J5 Enh Enh Promoter (57) Abstract: A genetically modified mouse is provided, wherein the mouse expresses an immunoglobulin light chain repertoire 0 characterized by a limited number of light chain variable domains. Mice are provided that express just one or a few immunoglobulin light chain variable domains from a limited repertoire in their germline. Methods for making light chain variable regions in mice, in cluding human light chain variable regions, are provided. Methods for making human variable regions suitable for use in multispe cific binding proteins, e.g., bispecific antibodies, are provided.