Patent EA-003297-B1 (EA)
Title: Cell line of sugar beet plant and sugar beet plants, having resistance to sulfonylurea herbicides and to imidazolinone herbicides, seed of indicated plant, methods of producing of indicated cell line and hybrid beet plants and using of imidazolinone herbicide for controlling weeds, in the fields with indicated sugar beet plants
Inventor: PENNER DONALD
IPC: C12N9/88
Filed: 1997-07-08
Abstract: 1. A cell line of sugar beet plant with a mutated acetolactate synthase gene, encoding the synthase wherein cytosine is substituted by thymine at position 562 and guanine is substituted by adenine at position 337, wherein the mutated cells have a resistance to both imidazolinone and sulfonylurea herbicides, regenerable to a sugar beet plant wherein the resistance is transmitted by conventional cross-breeding of regenerated plants with plants susceptible to said herbicides. 2. A cell line of sugar beet plant of claim 1 which has been derived from sensitive cells of a parent sugar beet plant, designated as CR1-B deposited as ATCC 97961 and having resistance to sulfonylurea herbicides and no resistance to imidazolinone herbicides, by cultivation of the sensitive cells in a culture medium with the imidazolinone herbicide to select for the mutated cells, deposited as ATCC 97535 (93R30B). 3. A sugar beet plant comprising cells with mutated acetolactate synthase gene, wherein cytosine is substituted by thymine at position 562 and guanine is substituted by adenine at position 337, wherein the mutated cells have a resistance to both imidazolinone and sulfonylurea herbicides wherein the resistance is transmitted by conventional cross-breeding of plants. 4. The plant by claim 3, which is regenerated from cell line according to claim 2. 5. A sugar beet plant seed according to any of claims 3-5. 6. A method of producing cell line of sugar beet plant according to claim 1 wherein cells of a parent sugar beet plant having resistance to sulfonylurea herbicides and no resistance to imidazolinone herbicides, by cultivation of the sensitive cells in a culture medium with the imidazolinone herbicide to select for the mutated cells having resistance both to imidazolinone and to sulfonylurea herbicides. 7. The method by claim 6, wherein cells received from parent sugar beet plant designated as 93R30 (SurSur) deposited as ATCC 97961 are cultivated with the following obtaining of the line, deposited as ATCC 97535 (93R30B). 8. A method of producing of cross-bred sugar beet plant having resistance both to imidazolinone and to sulfonylurea herbicides wherein the plant is regenerated from cells line of the sugar beet according to any of claims 1-2 and cross-breeding of the regenerated plant with sugar beet plant, sensitive to said herbicides, with the obtaining of cross-bred plant having resistance both to imidazolinone and to sulfonylurea herbicides. 9. A method of producing of cross-bred beet plant, except sugar beet, having resistance both to imidazolinone and to sulfonylurea herbicides wherein the plant is regenerated from cells line of the sugar beet by claim 1 of formula received in accordance with claim 6 cross-breeding of the regenerated plant with beet plant, except sugar beet, sensitive to said herbicides, with the obtaining of cross-bred plant having resistance both to imidazolinone and to sulfonylurea herbicides. 10. Using of an imidazolinone herbicide to control weeds growing in the fields with sugar beet plant according to any of claims 3-4 of formula.