Patent SG-11201408353Q-A (SG)
Title: An expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids
Inventor: CHANG MATTHEW WOOK
IPC: C12N15/63
Filed: 2013-06-12
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau „ (10) International Publication Number (43) International Publication Date ^ ^ WO 2013/191651 A1 27 December 2013 (27.12.2013) WIPO I PCT (51) International Patent Classification: C12N15/63 (2006.01) C12N15/70 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: PCT/SG2013/000242 12 June 2013 (12.06.2013) English English (30) Priority Data: 61/661,593 19 June 2012 (19.06.2012) (71) Applicant: NANYANG TECHNOLOGICAL UNIVER­ SITY [SG/SG]; 50 Nanyang Avenue, Singapore 639798 (SG). (72) Inventors: CHANG, Matthew Wook; c/o NANYANG TECHNOLOGICAL UNIVERSITY, 50 Nanyang Avenue, Singapore 639798 (SG). LO, Tat Ming Samuel; c/o NAN­ YANG TECHNOLOGICAL UNIVERSITY, 50 Nanyang Avenue, Singapore 639798 (SG). POH, Chueh Loo; c/o NANYANG TECHNOLOGICAL UNIVERSITY, 50 Nan­ yang Avenue, Singapore 639798 (SG). (74) Agent: VIERING, JENTSCHURA & PARTNER LLP; P.O. Box 1088, Rochor Post Office, Rochor Road, Singa­ pore 911833 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Ug (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (54) Title: AN EXPRESSION CONSTRUCT FOR SENSING CELL DENSITY AND SUBSTRATE AVAILABILITY AND ITS USE IN CONVERSION OF HYDROXYCINNAMIC ACIDS Time delay/ sensing cell density Sensing substrate acid) => Product (depends on substrate) o 0 i-H o\ m B i-H o CJ o & (57) Abstract: An Expression system; isolated nucleic acid molecule or host cell comprising: (i) A first gene en­ coding for a first enzyme linked to a first promoter, wherein the first promoter is a time delay promoter; (ii) A second gene encoding for a second enzyme capable of us­ ing the product generated by the first enzyme as a sub­ strate, wherein the second gene is operably linked to a second promoter, wherein the second promoter is indu­ cible by the product generated by the first enzyme; (iii) Optionally, a third gene encoding a transcription factor that represses expression of the second gene in the ab­ sence of the product generated by the first enzyme,