Patent SG-11201807940X-A (SG)
Title: Production of antigen-specific t-cells
Inventor: OELKE MATHIAS
IPC: A61K39/39
Filed: 2017-03-16
Abstract: International Patent Classification: A61K 39/39 (2006.01) A61K 35/17 (2015.01) A61K 47/48 (2006.01) C07K 14/725 (2006.01) (21) International Application Number: PCT/US2017/022663 (22) International Filing Date: 16 March 2017 (16.03.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/309,234 16 March 2016 (16.03.2016) US (71) Applicants: NEXIMMUNE, INC. [US/US]; 9119 Gaither Road, Ste A, Gaithersburg, MD 20877 (US). THE JOHNS HOPKINS UNIVERSITY [US/US]; 3400 N. Charles Street, Baltimore, MD 21218 (US). (72) Inventors: OELKE, Mathias; Neximmune, Inc., 9119 Gaither Road, Ste A, Gaithersburg, MD 20877 (US). SAN- TOS, Jose, Luis; Neximmune, Inc., 9119 Gaither Road, Ste A, Gaithersburg, MD 20877 (US). KIM, Sojung; Nex- immune, Inc., 9119 Gaither Road, Ste A, Gaithersburg, MD 20877 (US). SCHNECK, Jonathan; The Johns Hop- kins University, 3400 N. Charles Street, Baltimore, MD 21218 (US). KOSMIDES, Alyssa; The Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD 21218 (US). (74) Agents: HAYMAN, Mark, L. et al.; Morgan, Lewis & Bockius LLP, 1111 Pennsylvania Avenue, NW, Washing- ton, DC 20004 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, CF, CG, CI, CM, GA, GN, GQ, BJ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) [Continued on next page] (54) Title: PRODUCTION OF ANTIGEN-SPECIFIC T-CELLS FIG. 1 100nm DEXTRAN ANTI-BIOTIN COAT IRON OXIDE CORE SIGNAL 1 MHC-Ig DIMER- BIOTIN SIGNAL 2 CD28- BIOTIN 25 co g 20 15 10 5 2C T CELLS O O PMEL T CELLS CD8 CELL ACTIVATION ANTIGEN CD8 CELL PRESENTING CELL MHC-1 TCR SIGNAL 1 SIGNAL 2 CD80ICD88 CD28 ANTI-BIOTIN IRON-DEXTRAN NAN OPARTICL E W O 20 17 / 16 109 2 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/161092 Al 21 September 2017 (21.09.2017) WIPO I PCT (57) : The invention in various aspects provides for magnetic enrichment and/or expansion of antigen-specific T cells, al- lowing for identification and characterization of antigen-specific T cells and their T cell receptors (TCRs) for therapeutic and/or dia- gnostic purposes, as well as providing for production of antigen-specific engineered T cells for therapy. Incubation of paramagnetic nano-aAPCs in the presence of a magnetic field, either during enrichment and/or expansion steps, activates T cells through magnetic clustering of paramagnetic particles on the T cell surface. 1111111111111101110101011111010111110 01111111101011011101011111111111110111111 WO 2017/161092 Al 1111111111111101110111111111111111110111011111111111111111111111011111111111110111111 — before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))