Patent SG-11201809522W-A (SG)
Title: Method of nucleic acid sequence determination
Inventor: VIJAYAN KANDASWAMY
IPC: C12Q1/68
Filed: 2017-04-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 11111111111111111111111111111111111 1111111 111111111111 111111111111111111110111111 Organization (10) International Publication Number International Bureau (43) International Publication Date ......0\"\" WO 2017/190012 Al 02 November 2017 (02.11.2017) WIP0 I PCT (51) International Patent Classification: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, C12Q 1/68 (2006.01) HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, (21) International Application Number: KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, PCT/US2017/030135 MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (22) International Filing Date: SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, 28 April 2017 (28.04.2017) TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (30) Priority Data: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 62/329,489 29 April 2016 (29.04.2016) US TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, 62/444,733 10 January 2017 (10.01.2017) US EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (71) Applicant: OMNIOME, INC. [US/US]; 10575 Roselle TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, Street, San Diego, California 92121 (US). KM, ML, MR, NE, SN, TD, TG). (72) Inventors: VIJAYAN, Kandaswamy; c/o Omniome, Inc., 10575 Roselle Street, San Diego, California 92121 (US). Declarations under Rule 4.17: IYIDOGAN, Pinar; c/o Orrmiome, Inc., 10575 Roselle — as to applicant's entitlement to apply for and be granted a _ Street, San Diego, California 92121 (US). patent (Rule 4.17(0 — as to the applicant's entitlement to claim the priority of the (74) Agent: THOMAS, Tiffany B.; Kilpatrick Townsend & = —_ earlier application (Rule 4.17(iii)) Stockton LLP, 1100 Peachtree Street, Suite 2800, Atlanta, = Georgia 30309 (US). Published: — with international search report (Art. 21(3)) (81) Designated States (unless otherwise indicated, for every — with sequence listing part of description (Rule 5.2(a)) = kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, = (54) Title: METHOD OF NUCLEIC ACID SEQUENCE DETERMINATION = = 111111111 111111 11111 1 111111111 iiii 8 Bin ding ( nm) L ,;, t . 0 CBT 0 TDE /\"—• \ -ti / - -- f I \ • -• ,-1 • :: ) — _ _ e r\" --------1------LF\" ••- -- A \ C T 0.5 G ncorporation A \ C i ... , T ../ 0 0 200 400 600 800 1000 1200 1400 1-1 Reaction Progress (seconds) el FIG. 1 ,—, © © cr, 1-1 (57) : Provided are sequencing-by-binding methods of detecting cognate nucleotides using a crippled DNA polymerizing enzyme that possesses the ability to bind the next correct nucleotide downstream of a primer in a template-dependent fashion, but does 1-1 not possess the activity needed to promote phosphodiester bond formation. Use of the crippled DNA polymerase permits interrogation of one nucleotide at a time, without incorporation of any nucleotide. Labeled nucleotides, such as fluorescently labeled nucleotides, can el be used in conjunction with the crippled DNA polymerase to establish cognate nucleotide identity in a rapid manner.