Patent SG-11201808493Q-A (SG)
Title: Surface-based detection of nucleic acid in a convection flow fluidic device
Inventor: KHODAKOV DMITRIY
IPC: B01L7/00
Filed: 2017-03-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11M11111011101 11111111110101111101110101110101111001111111111111110111111 International Bureau 0.. .... .. (10) International Publication Number ..... ..or::,,, (43) International Publication Date WO 2017/172760 Al 5 October 2017 (05.10.2017) IN I PO I P CT (51) International Patent Classification: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, BOIL 7/00 (2006.01) C12Q 1/68 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (21) International Application Number: KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, PCT/US2017/024530 MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (22) International Filing Date: NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, 28 March 2017 (28.03.2017) RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (25) Filing Language: English ZA, ZM, ZW. (26) Publication Language: English (84) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of regional protection available): ARIPO (BW, GH, 62/314,909 29 March 2016 (29.03.2016) US GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (71) Applicant: WILLIAM MARSH RICE UNIVERSITY TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, [US/US]; 6100 Main St., Houston, TX 77005 (US). DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (72) Inventors: KHODAKOV, Dmitriy, A; c/o William Marsh LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Rice University, 6100 Main St., MS-142, Houston, TX SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, 77005 (US). ZHANG, David; c/o William Marsh Rice GW, KM, ML, MR, NE, SN, TD, TG). University, 6500 Main St., Suite 425, Houston, TX 77030 Published: (US). — with international search report (Art. 21(3)) (74) Agent: HIGHLANDER, Steven, L.; Parker Highlander before the expiration of the time limit for amending the PLLC, 1120 S. Capital Of Texas Highway, Building One, claims and to be republished in the event of receipt of Suite 200, Austin, TX 78746 (US). amendments (Rule 48.2(h)) Designated States (unless otherwise indicated, for every _ with sequence listing part of description (Rule 5.2(a)) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (81) — Il © IN N IN Il IN 1-1 (54) Title: SURFACE-BASED DETECTION OF NUCLEIC ACID IN A CONVECTION FLOW FLUIDIC DEVICE 0 N (57) : The present disclosure provides methods, composition and devices for performing convection-based PCR and non- ea.. ) enzymatic amplification of nucleic acid sequences. Techniques and reagents employed in these methods include toehold probes, ,7 1 .1 . 9 strand displacement reactions, Rayleigh-Benard convection, temperature gradients, multiplexed amplification, multiplexed detection, and DNA functionalization, in open and closed systems, for use in nucleic tests and assays.