Patent NZ-601950-A (NZ)
Title: Method for producing pyripyropene
Inventor: OYAMA KAZUHIKO
IPC: C12P17/18
Filed: 2011-01-19
Abstract: Disclosed is a method for producing pyripyropene A, characterized by culturing a microorganism in which at least one polynucleotide in (I) to (III) below or a recombinant vector comprising it/them is introduced with pyripyropene E and isolating pyripyropene A via pyripyropene O: (I) an isolated polynucleotide having at least one nucleotide sequence selected from the nucleotide sequences in (a) to (d) below: (a) a nucleotide sequence of SEQ ID NO: 266, (b) a nucleotide sequence which is capable of hybridizing with a sequence complementary to the nucleotide sequence of SEQ ID NO: 266 under stringent conditions, and which encodes a protein substantially equivalent to the protein encoded by the nucleotide sequence of SEQ ID NO: 266, (c) a nucleotide sequence of SEQ ID NO:266 in which one or more nucleotides are deleted, substituted, inserted or added, and which encodes a protein substantially equivalent to the protein encoded by the nucleotide sequence of SEQ ID NO:266, and (d) a nucleotide sequence which has at least 90% identity to a polynucleotide of the nucleotide sequence of SEQ ID NO: 266, and which encodes a protein substantially equivalent to the protein encoded by the nucleotide sequence of SEQ ID NO:266; (II) an isolated polynucleotide having a nucleotide sequence encoding at least one amino acid sequence selected from SEQ ID NOs:267 to 275 or a substantially equivalent amino acid sequence thereto; (III) an isolated polynucleotide having at least one nucleotide sequence selected from the nucleotide sequences in (1) to (4) below: (1) a nucleotide sequence in (a) to (i) below: (a) a nucleotide sequence from 3342 to 5158 of a nucleotide sequence shown in SEQ ID NO: 266, (b) a nucleotide sequence from 5382 to 12777 of a nucleotide sequence shown in SEQ ID NO: 266, (c) a nucleotide sequence from 13266 to 15144 of a nucleotide sequence shown in SEQ ID NO: 266, (d) a nucleotide sequence from 16220 to 18018 of a nucleotide sequence shown in SEQ ID NO: 266, (e) a nucleotide sequence from 18506 to 19296 of a nucleotide sequence shown in SEQ ID NO: 266, (f) a nucleotide sequence from 19779 to 21389 of a nucleotide sequence shown in SEQ ID NO: 266, (g) a nucleotide sequence from 21793 to 22877 of a nucleotide sequence shown in SEQ ID NO: 266, (h) a nucleotide sequence from 23205 to 24773 of a nucleotide sequence shown in SEQ ID NO:266, and (i) a nucleotide sequence from 25824 to 27178 of a nucleotide sequence shown in SEQ ID NO:266; (2) a nucleotide sequence which is capable of hybridizing with a sequence complementary to the nucleotide sequence in (1) under stringent conditions, and which encodes a protein substantially equivalent to a protein encoded by each nucleotide sequence; (3) a nucleotide sequence of a polynucleotide of the nucleotide sequence in (1) in which one or more nucleotides are deleted, substituted, inserted or added, and which encodes a protein substantially equivalent to the protein encoded by each nucleotide sequence; and (4) a nucleotide sequence which has at least 90% identity to a polynucleotide of the nucleotide sequence in (1), and which encodes a protein substantially equivalent to the protein encoded by each nucleotide sequence.