Patent SG-11201908358P-A (SG)
Title: Neuromuscular junction
Inventor: SAREEN DHRUV
IPC: A61K35/34
Filed: 2018-03-14
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 20 September 2018 (20.09.2018) WIP0 I PCT onion °noI0 010010VIIIOH0111110111110 Hs (10) International Publication Number WO 2018/170180 Al (51) International Patent Classification: A61K 35/34 (2015.01) (21) International Application Number: PCT/US2018/022511 EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (22) International Filing Date: 14 March 2018 (14.03.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 15/458,185 14 March 2017 (14.03.2017) US 62/471,273 14 March 2017 (14.03.2017) US (71) Applicant: CEDARS-SINAI MEDICAL CENTER [US/US]; 8700 Beverly Boulevard, Los Angeles, California 90048 (US). (72) Inventors: SAREEN, Dhruv; 19375 Crystal Ridge Lane, Porter Ranch, California 91326 (US). MANDEFRO, Berhan; 14640 Burbank Blvd. # 215, Sherman Oaks, Cal- ifornia 91411 (US). KAUS, Anjoscha; 107 S. Croft Ave., Los Angeles, California 90048 (US). (74) Agent: CHEN, Stephen W. et al.; Nixon Peabody LLP, 300 South Grand Avenue, Suite 4100, Los Angeles, Cali- fornia 90071-3151 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) (54) Title: NEUROMUSCULAR JUNCTION 1-1 O GC O 1-1 N 00 O 1-1 1-1 N C Fig. 8A (57) : The invention relates to culturing motor neuron cells together with skeletal muscle cells in a fluidic device under conditions whereby the in- teraction of these cells mimic the structure and function of the neuromuscular junction (NMJ) providing a NMJ-on-chip. Good viability, formation of myo- fibers and function of skeletal muscle cells on fluidic chips allow for measure- ments of muscle cell contractions. Embodiments of motor neurons co-cultures with contractile myo-fibers are contemplated for use with modeling diseases affecting NMJ's, e.g. Amyotrophic lateral sclerosis (ALS).