Patent EP-2713166-A1 (EP)
Inventor: THIEME ANDREAS
IPC: G01N35/00
Filed: 2012-10-01
Abstract: Laboratory machine (1) for program-controlled treatment of at least one laboratory sample, comprises (a) a treatment device (2) for the program-controlled treatment of the laboratory sample using several program parameters which are at least partially selected by an user, (b) an electronic control device (3) comprising at least one processor device (4) and at least one storage device (5), (c) a user interface device for manual input of data by a user and for displaying information comprising the data, and (d) at least one control program for controlling the laboratory machine. Laboratory machine (1) for program-controlled treatment of at least one laboratory sample, comprises (a) a treatment device (2) for the program-controlled treatment of the laboratory sample using several program parameters which are at least partially selected by an user, (b) an electronic control device (3) comprising at least one processor device (4) for data processing which comprises the execution of a control program for controlling the laboratory machine, and at least one storage device (5) for storing data, preferably the control program and the program parameters, (c) a user interface device for manual input of data by a user and for displaying information comprising the data, comprising a display, preferably a touchscreen display, and (d) at least one control program for controlling the laboratory machine, comprising instructions for (i) displaying at least one query area comprising at least one information area and at least one input area which is associated with the information area, in a first display area of the display, (ii) querying the program parameter which is required for the program-controlled execution of the treatment, in which an information for the program parameter is displayed in the information area, and an input from the user, preferably a touch of the input area, is captured in the input area, (iii) displaying at least one value which is input by the user for the program parameter in an information area of the second display area of the display, respectively after input of the program parameter by the user, (iv) displaying at least one or all values input by the user for the program parameters in the second display area, after the querying of the required program parameters, and (v) carrying out the treatment using the queried program parameters. Independent claims are also included for: (1) the program-controlled treatment of the laboratory sample using the laboratory machine by the control program in the laboratory machine, comprising the steps (i), (iia) carrying out progressive querying of program parameters which are required for the execution of the treatment, in which the information for the program parameter is displayed in the information area and an input from the user, preferably a touch on the input area, is captured in the input area, step (iii), step (iv) and step (v), where the laboratory machine comprises (a1) the treatment device for automatic treatment of the laboratory sample, where the treatment is automatically controlled using several program parameters, and the component (b), (c) and (d); (2) a storage medium comprising the control program for the laboratory machine, and instructions for (ia) displaying the query area in the first display area of the touchscreen display and carrying out the step (iia) and steps (iii), (iv) and (v); and (3) use of the laboratory machine or the method for carrying out the laboratory task for treating the laboratory sample, where the laboratory task comprises nucleic acid purification, preferably purification of genomic DNA from whole blood using Eppendorf MagSep Blood gDNA kit (RTM: Reagent kit for purification of genomic DNA from whole blood) (MagSep blood gDNA), purification of genomic DNA from living tissue using Eppendorf MagSep Tissue gDNA kit (RTM: Reagent kit for purification of genomic DNA from living tissue) (MagSep Tissue gDNA), purification of viral RNA or DNA from cell-free body fluids using the Eppendorf MagSep Viral DNA/RNA kit (RTM: Reagent kit for purification of viral RNA or DNA from cell-free body fluids) (MagSep Viral DNA/RNA), and PCR applications, preferably production of at least one PCT mastermix from pre-mixes or individual constituents, preferably buffer, polymerase, deoxynucleotide triphosphates, primers or markers (compose mastermix), where the volume of each required constituent is calculated by the control device, preferably by program module which is associated with the compose mastermix treatment, dilution of at least two DNA/RNA samples for obtaining the same concentration in all samples (normalize concentrations), where the desired concentrations is input manually by the user, or imported from a file, or dilution of at least one DNA/RNA standard sample with a known concentration in succession for producing a calibration curve which is usable for quantitative PCR (create dilution series) or producing reaction processes in which several samples are combined with at least one mastermix (setup reactions), where optionally the reactions are also used for replication of DNA/RNA.