Patent SG-11201903519U-A (SG)
Title: Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations
Inventor: MIKKELSEN TARJEI
IPC: C12Q1/68
Filed: 2017-10-18
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property MD 1101111 0 DOI HIM 011101 0 01111H111111111111111111111111111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ......0\" WO 2018/075693 Al 26 April 2018 (26.04.2018) WIP0 I PCT _ (51) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) (71) (72) International Patent Classification: (81) Designated States (unless otherwise indicated, for every Cl 2N 15/10 (2006.01) C12Q 1/68 (2018.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, PCT/US2017/057269 CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 18 October 2017 (18.10.2017) KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, English OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, Priority Data: TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 62/410,326 19 October 2016 (19.10.2016) US (84) Designated States (unless otherwise indicated, for every 62/490,546 26 April 2017 (26.04.2017) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, Applicant: 10X GENOMICS, INC. [US/US]; 7068 Koll UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, Center Parkway, Suite 401, Pleasanton, CA 94566 (US). TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Inventors: MIKKELSEN, Tarjei, Sigurd; 4700 Tassajara EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, Road, Apt. 3412, Dublin, CA 94568 (US). BELGRADER, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, Phillip; 2263 Cabernet Way, Livermore, CA 94450 (US). TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ZHENG, Xinying; 1763 Comstock Lane, San Jose, CA KM, ML, MR, NE, SN, TD, TG). 95124 (US). Agent: LAM, Amy et al.; Wilson Sonsini Goodrich & Rosati, 650 Page Mill Road, Palo Alto, CA 94304-1050 (US). (74) = = (54) CELL POPULATIONS i r ) ( 9081 Title: METHODS AND SYSTEMS FOR BARCODING NUCLEIC ACID MOLECULES FROM INDIVIDUAL CELLS OR Figure 9A (57) : The present disclosure provides methods, compositions and systems for analyzing individual cells or cell populations through a parti- tioned analysis of contents of individual cells or cell populations, such as cancer cells and cells of the immune system. Individual cells or cell popula- tions may be co-partitioned with processing reagents for accessing cellular contents, and for uniquely identifying the content of a given cell or cell pop- ulation, and subsequently analyzing the content of the cell and characteriz- ing it as having derived from an individual cell or cell population, includ- ing analysis and characterization of nucleic acid(s) from the cell through sequencing. = _ = - = - = _ _ = 920 sso _ = = 922 V — 1-1 M C:r IN 0 --.... Il © ei J 958 I_VIL.112.1V1124 , 1111, C [Continued on next page] WO 2018/075693 Al MIDEDIMOMMIDEFIERIMIONNIMMOVOIMIE Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event amendments (Rule 48.2(h)) of receipt of