Patent EA-037466-B1 (EA)
Title: Device and method for express assessment of functional state of haemostasis system
Inventor: TYUTRIN IVAN ILLARIONOVICH
IPC: G01N33/48
Filed: 2019-08-23
Abstract: The invention relates to the field of medicine, in particular, to devices and methods for express evaluation of functional state of a hemostatic system. A device for express evaluation of the state of a hemostatic system, comprising a measuring chamber, electrodes connected to a frequency generator and a recording unit, wherein the measuring chamber and the electrodes are located on the base of a cartridge connected to the recording unit via a connector by means of a group of contact electrodes; the measuring chamber is located inside a piezoelectric detector made as a hollow cylinder, the outer surface of which is fitted with an external electrode divided by symmetrical slots into two equal parts, one part being a generator, and the other part being an ultrasonic oscillation receiver; the inner surface of the measuring chamber is fitted with an internal electrode, the central contact of which is built in the cartridge body; the recording unit includes a power system, a digital signal generator, a signal correction circuit, a microcontroller, an analog signal amplification and conversion board, a display, a keyboard, a Wi-Fi module, a USB port for the output to a computer, a non-volatile clock; the output of the power system unit is connected to the microcontroller input, the microcontroller output is connected to the display input, the microcontroller output is connected to the digital signal generator input; also, the digital signal generator output is connected to the microcontroller input, the digital signal generator output is connected to the signal correction circuit input, the signal correction circuit output is connected to the cartridge input, the cartridge output is connected to the input of the analog signal amplification, conversion and decoding board, the output of the analog signal amplification, conversion and decoding board is connected to the microcontroller input, the keyboard outputs are connected to the microcontroller inputs, the outputs of the real-time non-volatile clock, Wi-Fi module, USB output to a computer and the display are connected to the microcontroller inputs; also, the microcontroller outputs are connected to the inputs of the real-time non-volatile clock, Wi-Fi module, USB output to a computer and the display input. When applying the method for express evaluation of the functional state of a hemostatic system, a whole blood sample placed into the measuring chamber of the device according to claim 1 is exposed to ultrasonic oscillations at a frequency of 150-160 kHz; after that, an echo coagulogram is recorded, and, in time intervals T1=0.3 min, T2=2 min, T3=4 min, T4=5 min, determined are the magnitude of amplitude indicators 0A1, 0A2, 0A3, A04, respectively, the slope of curve between points A1 and A2 - ∟, the integrated index is calculated: area of S sector limited on the one side by a curve segment between points A1 and A4 and on the other side by X-axis; then, using a software, their values are compared with the reference equivalent values determined for healthy volunteers of various age groups, and upon an increase in the integrated index by at least 20%, the state of hypercoagulation is determined, and upon its decrease by at least 20%, the state of hypocoagulation is determined. The novel technical result is improvement of the method efficiency due to a capability of on-line monitoring of the clotting process in the real-time mode in the studied blood micro-volumes, improvement of convenience and availability of this study for a patient and a doctor.