Patent SG-11201901621V-A (SG)
Title: Development of spinal cord on a microfluidic chip
Inventor: KERNS S
IPC: C12N15/85
Filed: 2017-08-29
Abstract: PLASMA TREAT CHIPS `AIR PLUG TOP CHANNEL * COAT BOTTOM CHANNEL WITH COL IV:FN, INCUBATE HOUR AT 37 ° C ' REMOVE COL IV:FN * DRY CHIP FOR 15 MINUTES IN VACUUM DESICCATOR * COAT TOP CHANNEL WITH LAMININ, INCUBATE 2 HOURS AT 37 ° C * PLATE iBMECs ON BOTTOM CHANNEL * WASHED APICAL CHAMBER WITH NEURAL MEDIUM * SEED OF NEURAL CELLS * INCUBATE OVER NIGHT AT 37 ° C FIG. 1 W O 20 18/044885 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 08 March 2018 (08.03.2018) WIPO I PCT olimion °nolo III o Ho mil oimIE (10) International Publication Number WO 2018/044885 Al (51) International Patent Classification: C12N 5/071 (2010.01) C12N 5/0797 (2010.01) C12N 5/079 (2010.01) Cl 2N 15/85 (2006.01) C12N 5/0793 (2010.01) (21) International Application Number: PCT/US2017/049115 (22) International Filing Date: 29 August 2017 (29.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/380,780 29 August 2016 (29.08.2016) US PCT/US2016/057724 19 October 2016 (19.10.2016) US 15/352,289 15 November 2016 (15.11.2016) US (71) Applicants: EMULATE, INC. [US/US]; 27 Drydock, Boston, MA 02210 (US). CEDARS-SINAI MEDICAL CENTER [US/US]; 8700 Beverly Blvd, Los Angeles, CA 90048 (US). (72) Inventors: KERNS, Jordan; 7 Heritage Drive, Reading, MA 01867 (US). WEN, Norman; 4975 Washington Street, Unit 118, West Roxbury, MA 02132 (US). LUCCHESI, Carol; 16 Carroll Ct., Westwood, MA 02090 (US). HINO- JOSA, Christopher; 69 Holworthly Street, Cambridge, MA 02138 (US). FRASER, Jacob; 19 Kington Street, Apt. 2, Somerville, MA 02144 (US). HAMILTON, Geraldine; 509 East 2nd Street, Unit 7, Boston, MA 02127 (US). VATINE, Gad; 508 1/2 South Ogden Drive, Los Ange- les, CA 90026 (US). SANCES, Sam; 2021 Ocean Ave, Apt. 223, Santa Monica, CA 90405 (US). SVENDSEN, Clive; 15242 Friends Street, Pacific Palisades, CA 90272 (US). LEVNER, Daniel; 55 Sewall Ave 5C, Brookline, MA 02446 (US). SAREEN, Dhruv; 19375 Crystal Ridge Lane, Porter Ranch, CA 91326 (US). (74) Agent: CARROLL, Peter, G. et al.; Medlen & Carroll, LLP, 1440 Broadway, Suite 1010, Oakland, CA 94612 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (54) Title: DEVELOPMENT OF SPINAL CORD ON A MICROFLUIDIC CHIP (57) : The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barri- er. Culture of such cells in a microfluidic device, whether alone or in combi- nation with other cells, drives maturation and/or differentiation further than existing systems. [Continued on next page] WO 2018/044885 Al MIDEDIMOMMIDIREEMOMMOHEHMODEVOIS SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))