Patent SG-11201810871W-A (SG)
Title: Methods of producing modified natural killer cells and methods of use
Inventor: CHILDS RICHARD
IPC: C12N5/0783
Filed: 2017-07-25
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 February 2018 (01.02.2018) WIP0 I PCT onion °nolo III olom Immo mi Eno (10) International Publication Number WO 2018/022646 Al (51) International Patent Classification: C12N 5/0783 (2010.01) (21) International Application Number: PCT/US2017/043774 (22) International Filing Date: 25 July 2017 (25.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/366,493 25 July 2016 (25.07.2016) US (71) Applicant: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DE- PARTMENT OF HEALTH AND HUMAN SERVICES [US/US]; National Institutes Of Health, Office of Technol- ogy Transfer, 6011 Executive Boulevard, Suite 325„ Msc 7660, Bethesda, MD 20852-7660 (US). (72) Inventors: CHILDS, Richard, W.; Bldg 10 CRC Rm 35330, 10 Center Drive, Bethesda, MD 20892 (US). CHIN- NASAMY, Dhanalakshmi; Crc Bldg 10, Room #5e-5264, 10 Center Drive, Bethesda, MD 20892 (US). ALLAN, David, S.J.; Building 10-CRC, Room 5-5264, 10 Center Drive, Bethesda, MD 20892 (US). (74) Agent: GRAF, Susan, W.; Klarquist Sparkman, LLP, One World Trade Center, Suite 1600, 121 SW Salmon Street, Portland, OR 97204 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (54) Title: METHODS OF PRODUCING MODIFIED NATURAL KILLER CELLS AND METHODS OF USE (57) : Disclosed herein are method of producing NK cells that include one or more heterologous nucleic acids. The methods include culturing a population of isolated NK cells in the presence of one or more cytokines to produce a population of activated NK cells. The population of activated NK cells are transduced with a viral vector comprising the one or more heterologous nucleic acids, for example by contacting the activated NK cells with viral particles including the viral vector. The resulting transduced NK cells are then cultured in the presence of one or more cytokines, and optionally in the presence of irradiated feeder cells, to produce a population of expanded transduced NK cells. Also disclosed are methods of treating a subject with a disorder (such as a tumor or hyperproliferative disorder) by administering to the subject NK cells produced by the methods described herein.