Patent SG-11201805162V-A (SG)
Title: Plasmid constructs for heterologous protein expression and methods of use
Inventor: PIERCE ROBERT H
IPC: A61K39/00
Filed: 2016-12-16
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization 1111111101111011101010111110101111101110101101111111111111101111101111011111 International Bureau 0.. .... .. (10) International Publication Number (43) International Publication Date ..... ...r .....1 WO 2017/106795 Al 22 June 2017(22.06.2017) WIPO I PCT (51) International Patent Classification: (72) Inventor: PIERCE, Robert H.; 877 Island Avenue, Unit C12N 15/85 (2006.01) A61P 35/00 (2006.01) 1002, San Diego, CA 92101 (US). C12N 15/79 (2006.01) A61K 48/00 (2006.01) C12N 15/63 (2006.01) A61K 39/39 (2006.01) (74) Agent: MOHAN-PETERSON, Sheela; 5820 Nancy C12N 15/09 (2006.01) A61K 39/00 (2006.01) Ridge Drive, San Diego, CA 92121 (US). (21) International Application Number: (81) Designated States (unless otherwise indicated, for every PCT/US2016/067388 kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (22) International Filing Date: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, 16 December 2016 (16.12.2016) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (25) Filing Language: English KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (26) Publication Language: English MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (30) Priority Data: RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, 62/269,702 18 December 2015 (18.12.2015) US TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, 62/375,245 15 August 2016 (15.08.2016) US ZA, ZM, ZW. (71) Applicant: ONCOSEC MEDICAL INCORPORATED (84) Designated States (unless otherwise indicated, for every [US/US]; 5820 Nancy Ridge Drive, San Diego, CA 92121 kind of regional protection available): ARIPO (BW, GH, (US). GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (72) Inventors; and TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (71) Applicants : CAMPBELL, Jean [US/US]; 839 Northeast TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, 92nd St., Seattle, WA 98115 (US). CANTON, David A. DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, [US/US]; 13023 Neddick Ave., Poway, CA 92064 (US). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). [Continued on next page] (54) Title: PLASMID CONSTRUCTS FOR HETEROLOGOUS PROTEIN EXPRESSION AND METHODS OF USE (57) : Provided are plasmid vector constructs encoding multiple immunomod- ulatory proteins where each protein or component thereof can be expressed utiliz- ing appropriate promotors and/or transla- tion modifiers. Additional immunomodu- latory proteins and genetic adjuvants con- taining shared tumor antigens can be added to further therapeutic potential as well as allow tracking of therapeutic treatment. pOMI A, 112 Also provides are methods of expressing the plasmid constructs. 11 ir) CT IN FIG .I 11 Ir ---- 11 ei O WO 2017/106795 Al MIDEDIMOMOIDEIHMEMOMOMENOMMODEVOIS Declarations under Rule 4.17: Published: — as to applicant's entitlement to apply for and be granted — with international search report (Art. 21(3)) a patent (Rule 4.17(0) — before the expiration of the time limit for amending the — as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) — with sequence listing part of description (Rule 5.2(a))