Patent SG-11201806310T-A (SG)
Title: Linked perfusion to continuous-flow stirred-tank reactor cell culture system
Inventor: HILLER GREGORY WALTER
IPC: C12P21/00
Filed: 2017-01-25
Abstract: International Patent Classification: C12M 1/00 (2006.01) C12N 5/00 (2006.01) C12P 21/00 (2006.01) (21) International Application Number: PCT/US2017/014814 (22) International Filing Date: 25 January 2017 (25.01.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/287,194 26 January 2016 (26.01.2016) US 62/331,619 4 May 2016 (04.05.2016) US (71) Applicant: BOEHRINGER INGELHEIM INTERNA- TIONAL GMBH [DE/DE]; Boehringer Ingelheim GmbH, Corporate Patents, Binger Strasse 173, 55216 Ingelheim am Rhein (DE). (72) Inventors; and (71) Applicants US only): HILLER, Gregory Walter [US/US]; c/o VP, IP, Legal, Boehringer Ingelheim USA Corp., 900 Ridgebury Road, P.O. Box 368, Ridgefield, Connecticut 06877-0368 (US). GAGNON, Matthew Paul [US/US]; c/o VP, IP, Legal, Boehringer Ingelheim USA Corp., 900 Ridgebury Road, P.O. Box 368, Ridgefield, Connecticut 06877-0368 (US). COFFMAN, Jonathan [US/US]; c/o VP, IP Legal, Boehringer Ingelheim USA Corp., 900 Ridgebury Rd., P.O. Box 368, Ridgefield, Con- necticut 06877-0368 (US). (74) Agents: MORRIS, Michael P. et al.; Boehringer Ingel- heim Pharmaceuticals, Inc., 900 Ridgebury Road, P.O. Box 368, Ridgefield, CT 06877-0368 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). arations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.1700) Decl [Continued on next page] (54) Title: LINKED PERFUSION TO CONTINUOUS-FLOW STIRRED-TANK REACTOR CELL CULTURE SYSTEM HIGH VIABILITY CONTINUOUS OR SEMI CONTINUOUS CELL TRANSFER -1. '\" CELL WA S C E T L E L c .F u R L E Tu E RE RETENTION DEVICE REC RCULATION LOOP C><) CONSTANT VOLUME N-I PERFUSION BIOREACTOR HIGHLY CONCENTRATED FEED CELL CONTAINING CONTINUOUS HARVEST FRESH MEDIUM W O 20 17 / 13 2185 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/132185 Al 3 August 2017 (03.08.2017) WIPO I PCT CONTINUOUS STIRRED.TANK BIOREACTOR (CSTR) OR C HEMOSTAT Figure 1 (57) : Methods of protein production in a linked culture and production bioreactor system are provided. Such methods in- clude a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculat- ing a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest. 11111111111111011101111111111101011111011101110111010111H11111111111110111111 WO 2017/132185 Al 1#11101MOHNOIRME30111101010EIMEMOVOIS Published: — with international search report (Art. 21(3))