Patent EA-001036-B1 (EA)
Title: Unmodified fungal oil mortierella sp., method for production thereof, method of providing arachidonic acid to infant formula, infant formula containing arachidonic acid, method of providing a human with supplemental amount of arachidonic acid and cosmetic composition
Inventor: KYLE DAVID J
IPC: A23L1/30
Filed: 1996-01-03
Abstract: 1. An unmodified oil of fungus Mortierella sp., comprising at least about 40% arachidonic acid (ARA) in the triglyceride and no more than one fifth as much eicosapentaenoic acid (EPA) as arachidonic acid (ARA). 2. An unmodified fungal triglyceride oil according to claim 1, the oil comprising at least 50% arachidonic acid (ARA) in the triglyceride and essentially no eicosapentaenoic acid (EPA). 3. The unmodified fungal triglyceride oil of claim 1 or 2, wherein the oil contains no more than one tens as much at least 50% arachidonic acid (ARA). 4. A method for the production of an unmodified fungal oil, comprising arachidonic acid according to any one of claims 1-3, comprising: - cultivating Mortierella sp. in an aerated fermentor containing culture medium wherein a carbon source in an amount equivalent to at least 80 g/L glucose and a nitrogen source in an amount equivalent to at least 15 g/L yeast extract are added to the culture medium over the course of the fermentation; - maintaining the pH between 5 and 6 at the beginning of the cultivation; - maintaining the pH between 7 and 7.5 at the end of the cultivation; - harvesting biomass from the fermentor and - recovering said arachidonic acid-containing oil from said biomass. 5. The method of claim 4, wherein the dissolved oxygen level in the culture medium is at least 35% of the air saturation level. 6. The method of claim 4, wherein the nitrogen source is divided into two or more aliquots which are fed into the fermentor at different times, at least one aliquot being fed into the fermentor at a time different from the time the carbon source is placed into the fermentor. 7. The method according to any one of claims 4-6, wherein the Mortierella sp. is M. alpina. 8. The method according to any one of claims 4-7, further wherein crude arachidonic acid-containing oil is recovered from the biomass by extraction with a non-polar solvent and the crude oil is clarified by extraction with a polar organic solvent. 9. The method of claim 8, wherein the non-polar solvent is hexane. 10. The method of claim 8, wherein the polar solvent is acetone, ethanol and isopropyl alcohol. 11. A method of providing arachidonic acid (ARA) to an infant formula which comprises adding an unmodified fungal oil in an amount comparable to the amount in human breast milk wherein oil is added to infant formula as an unmodified fungal oil according to any one of claims 1-3. 12. Infant formula comprising arachidonic acid (ARA) to an infant formula which comprises adding an unmodified fungal oil in an amount comparable to the amount in human breast milk wherein an unmodified fungal oil is added to infant formula according to any one of claims 1-3. 13. A method of providing a human with supplemental arachidonic acid (ARA) comprising administering to a human in need of supplemental arachidonic acid (ARA) a composition containing unmodified fungal oil according to any one of claims 1-3 in an amount effective to provide supplemental arachidonic acid (ARA) to said human. 14. The method of 13, wherein comprising administering to a human in need of supplemental arachidonic acid (ARA) a composition containing said oil in an amount that supplies 0.2-0.8 g arachidonic acid (ARA)/day. 15. The method according to any one of claims 13-14, wherein said composition is administered enterally. 16. The method according to any one of claims 13-14, wherein said composition is administered parenterally. 17. The method according to any one of claims 13-14, wherein said composition is administered topically. 18. The method according to any one of claims, 13-14, wherein said human is a pregnant or nursing woman. 19. The method according to any one of claims 13-14, wherein said human in need of supplemental arachidonic acid (ARA) is suffering from a neurological disorder. 20. The method according to claim 19, wherein the neurological disorder is tardive dyskinesia, schizophrenia, or a peroxisomal disorder. 21. The method according to any one of claims 13-14, wherein said composition is administered to a human in need suffering from a disease condition associated with reduced serum level of arachidonic acid (ARA). 22. The method according to claim 21, wherein the disease is a liver disease, phenyl ketonuria or cystic fibrosis. 23. A cosmetic composition comprising unmodified fungal oil, wherein comprises oil as said oil according to any one of claims 1-2, wherein said oil is present in said composition in an amount effective to assist in maintaining skin tone when said composition is applied topically. The Eurasian patent is valid in all Contracting stated except for AM, KG, MD.