Patent NZ-572364-A (NZ)
Title: Tomato plants having higher levels of resistance to botrytis
Inventor: FINKERS HENDRIKUS JOHANNES
IPC: C12N15/82
Filed: 2007-04-25
Abstract: Disclosed is a method of producing a Botrytis-resistant tomato plant, said method comprising the steps of: a) providing a Botrytis-resistant donor tomato plant, preferably a Botrytis-resistant plant of the species S. habrochaites, more preferably a Botrytis-resistant plant of the line S. habrochaites LYC 4/78; b) transferring nucleic acid from said donor plant to one or more Botrytis-susceptible recipient tomato plants, preferably a plant of the species S. lycopersicum, wherein said transfer results in the introduction of genomic material from the donor plant in the corresponding region of the genome of said one or more recipient plants; c) selecting from amongst said recipient tomato plants a plant that comprises within its genome at least one QTL for Botrytis-resistance derived from said Botrytis-resistant donor tomato plant, wherein said selection comprises detecting on chromosome 4, 6, 9, 11 and/or 12 of said recipient tomato plant at least one genetic marker linked to said at least one QTL for Botrytis-resistance, - wherein the location of said QTL on chromosome 4 of said plant is indicated by a genomic region comprising the genetic markers CT50, C2At1g74970, P14M49-283e, P14M48-74e, P14M50-67e, CT1739 and P14M50-85h on chromosome 4 of S. habrochaites or on chromosome 4 of S. lycopersicum wherein the location of said QTL on chromosome 6 of said plant is indicated by a genomic region comprising the genetic markers P22M50-188h, P14M48-521e, P15M48-386h, P18M51-199h, P18M51-103h, P22M50-103e, P18M51-388e, P15M48-395e, P22M50-124e, P14M48-160e and P22M50-513h on chromosome 6 of S. habrochaites or on chromosome 6 of S. lycopersicum, - wherein the location of said QTL on chromosome 9 of said plant is indicated by a genomic region comprising the genetic markers P18M50-141, P14M49-240, TG254, TG223, TG10, P18M50-134h, P14M49-243h, P18M50-599, P14M60-222h, P22M51-417h, P14M50-174h, P14M60-157h, P14M60-107h, P15M48-138h, P14M48-113h, Tm2a, P18M51-146h, P14M48-282h and P14M50-276h on chromosome 9 of S. habrochaites or on chromosome 9 of S. lycopersicum, - wherein the location of said QTL on chromosome 11 of said plant is indicated by a genomic region comprising the genetic markers P14M60-215e, P14M61-173h, P14M50-307h, TG47, P14M50-29xCD, P18M51-358h, P18M50- 27xCD, P18M51-136h, P22M50-488h, TG393, P14M61-396h, P22M51-235h and P22M51-174e on chromosome 11 of S. habrochaites or on chromosome 11 of S. lycopersicum, and - wherein the location of said QTL on chromosome 12 of said plant is indicated by a genomic region comprising the genetic markers CT19, TG68, P14M48-411e, P18M50-244h, P18M50-273h, P14M61-420h, P14M61-406h, P14M61-223h, P14M60-193h, P22M51-314h, TG565, P14M48-172h, P22M50-321e, P14M60-219e, P14M48-153h, P22M50-97h, TG296, P22M50-131h and P22M51-135h, preferably by a genomic region comprising the genetic markers P14M61-420h, P14M61-406h, P14M61-223h, P14M60-193h, P22M51-314h, TG565, P14M48-172h, P22M50-321e, P14M60-219e, P14M48-153h, P22M50- 97h, TG296, and P22M50-131h on chromosome 12 of S. habrochaites or on chromosome 12 of S. lycopersicum.