Patent SG-11201407295Y-A (SG)
Title: Biocatalysts and methods for hydroxylation of chemical compounds
Inventor: CHEN HAIBIN
IPC: C12N9/04
Filed: 2013-05-07
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 14 November 2013 (14.11.2013) WIPOIPCT (10) International Publication Number WO 2013/169725 A3 (51) International Patent Classification: C12N 9/04 { 2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) Priority Data: 61/644,135 8 May 2012 (08.05.2012) (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Drive, Redwood City, California 94063 (US). (74) Agents: WHITING, Adam, K. et al.; CODEXIS, INC., 200 Penobscot Drive, Redwood City, California 94063 (US). PCT/US2013/039874 (81) Designated States (unless otherwise indicated , for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. 7 May 2013 (07.05.2013) English English US (72) Inventors: CHEN, Haibin; 61 Science Park Rd, #03- 15/24 THe Galen, Singapore Science Parkll, Singapore 117525 (SG). BONG, Yong Koy; BLK 652C #14-406, Jurong West St 61, Singapore 643652 (SG). CABIROL, Fabien; Bergerstrasse 24, 40213 Dusseldorf (DE). GO- HEL, Anupam; Pinus Hijau IV, No. 12, Meadow green, Lippo-cikarang, Bekasi, 17550 (ID). LI, Tao; 2521 Spindehill Drive Apt 4, Cincinnati, Ohio 45230 (US). MOORE, Jeffrey C.; 367 Orenda Circle, Westfield, New Jersey 07090 (US). QUINTANAR-AUDELO, Martina; 6.GhimMoh Rd #14-200, Singapore 270006 (SG). YANG, Hong; 61 Science Park Rd, #03-15/24 The Galen, Singa­ pore Science Parkll, Singapore 117525 (SG). COLLIER, Steven, J.; 22 Burroughs Rd, Lexington, Massachusetts 02420 (US). SMITH, Derek; 12 Holland Avenue #10-31, 272012 Singapore, Singapore 117525 (SG). (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) (88) Date of publication of the international search report: 6 March 2014 CJ i> o\ i-H cn i-H o CJ (54) Title: BIOCATALYSTS AND METHODS FOR HYDROXYLATION OF CHEMICAL COMPOUNDS (57) Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production ofhydroxylated com­ pounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hy­ droxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active phar­ maceutical agents. The present disclosure provides engineered proline hydroxylase biocatalysts, polynucleotides encoding the biocatalysts. methods of their preparation, and processes for preparing hydroxylated compounds using these engineered biocatalysts.