Patent SG-11201811104T-A (SG)
Title: Screening kit and method
Inventor: BOCCHI MASSIMO
IPC: G01N35/10
Filed: 2017-06-14
Abstract: gaudhumidity Gontwiled hilicroscOp iye () INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 21 December 2017 (21.12.2017) WIPO I PCT omit VIII two III OH VIII ioo Illoono iflo oimIE (10) International Publication Number WO 2017/216739 Al (51) International Patent Classification: G01N 35/10 (2006.01) GO1N 15/10 (2006.01) G01N 15/14 (2006.01) (21) International Application Number: PCT/IB2017/053530 (22) International Filing Date: 14 June 2017 (14.06.2017) (25) Filing Language: Italian (26) Publication Language: English (30) Priority Data: 102016000061106 14 June 2016 (14.06.2016) IT 102016000122158 01 December 2016 (01.12.2016) IT (71) Applicant: CELLPLY S.R.L. [IT/IT]; Via Fondazza, 53, 40125 Bologna (IT). (72) Inventors: BOCCHI, Massimo; c/o Cellply S.r.l., Via Fondazza, 53, 40125 Bologna (IT). FAENZA, Andrea; c/o Cellply S.r.l., Via Fondazza, 53, 40125 Bologna (IT). ROC- CHI, Laura; c/o Cellply S.r.l.., Via Fondazza, 53, 40125 Bologna (IT). BISCARINI, Dario; c/o Cellply S.r.l., Via Fondazza, 53, 40125 Bologna (IT). PECORARI, Nicola; c/o Cellply S.r.l., Via Fondazza, 53, 40125 Bologna (IT). (74) Agent: RIGAMONTI, Dorotea et al.; c/o Jacobacci & Partners S.p.A., Via Senato, 8, 1-20121 Milano (IT). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (54) Title: SCREENING KIT AND METHOD imagif) analysis Fla 28 (57) : The present invention relates to a method for the large-scale high-content analysis of biological samples, implemented in a reversed open microwell system which comprises an array of open microwells, at least one microchannel, at least one input port for reagents and/or for one or more biological samples and at least one output port for them, said input and output ports being in microfluidic communication with one or more of said microchannels, wherein said microchannel has a cross- section area of micrometric dimensions and provides fluid to said microwells, wherein said reversed open microwell system is inserted in an automated management system which comprises the following features: an incubator at controlled temperature, humidity and CO2, fluid dispensing system, phase- contrast and fluorescence image acquisition. The present invention further relates to a kit for introducing fluids in a microfluidic device which comprises a tip, and to a microfluidic device which comprises at least one microchannel and an input region which comprises at [Continued on next page] WO 2017/216739 Al MIDEDIMOMOIDEIRMEMODIMMINEIIMIDEVOIS OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) least one vertical channel, said tip and said vertical channel being dimensioned so to produce an interference coupling therebetween. In a further aspect, a system for discharging one or more fluids in a microfluidic device is described. Said discharge region comprises: a discharge container in fluidic connection with said microfluidic device through at least one discharge channel and an output port.