Patent DE-102011084981-A1 (DE)
Title: Demonstrating effectiveness of cosmetic or pharmaceutical active substances, comprises cultivating cell or tissue model, determining expression of gene from mitochondrial enzyme and applying active substance on cell or tissue models
Inventor: GIESEN MELANIE
IPC: C12Q1/00
Filed: 2011-10-21
Abstract: Demonstrating the effectiveness of cosmetic or pharmaceutical active substances, comprises (a) cultivating a cell or tissue model; (b) determining the expression or activity of at least one gene or protein from the group of mitochondrial enzyme complexes and related transcription factors to define the before-status; (c) singly or repeatedly applying an active substance on the cell or tissue culture models for some time; (d) determining the expression or activity of at least one gene or protein; and (e) assessing the active substance based on difference between after-state and before-status. Test method for demonstrating the effectiveness of cosmetic or pharmaceutical active substances, comprises either (a) cultivating a cell or tissue model; (b) determining the expression or activity of at least one gene or protein from the group of mitochondrial enzyme complexes and related transcription factors to define the before-status; (c) singly or repeatedly applying an active substance on the cell or tissue culture models for some time; (d) determining the expression or activity of at least one gene or protein from the group of mitochondrial enzyme complexes and related transcription factors to determine the after status; and (e) assessing the active substance based on the difference between after-state and before-status, where the applied active substance stimulates the gene or protein; or recovering of at least one hair to give the hair follicle from the scalp of an individual by plucking; determining the expression or activity of at least one gene or protein from the group of mitochondrial enzyme complexes and related transcription factors to define the before-status in at least one hair; singly or repeatedly applying the active substance on the hairy skin of this individual; recovering at least one other hair to give the hair follicle from the scalp of an individual by plucking; determining the expression or activity of at least one gene or protein from the group of mitochondrial enzyme complexes and related transcription factors to determine the after status in at least one hair; and assessing the active substance based on the difference between after-status and before-status. An independent claim is included for individualized hair treatment for improving hair growth, hair structure and hair pigmentation, in vivo, comprising obtaining at least one hair to give the hair follicle from the scalp of an individual by plucking for extracorporeal analysis of hair follicle cells, examining at least one hair follicle on the expression or activity of at least one gene or protein from the group of mitochondrial enzyme complexes and related transcription factors, comparing the actual value of at least the hair-specific factor with predetermined desired values, and treating the hair of the individual with cosmetic products which improve the expression or activity of at least one gene or protein from the group of mitochondrial enzyme complexes and related transcription factors.