Patent EA-000736-B1 (EA)
Title: Bioprocess for production of dipeptide based compounds
Inventor: BORNEMAN SCOTT W
IPC: C07K5/06
Filed: 1996-04-08
Abstract: 1. A process for producing a dipeptide Ala-Pro, representing a) expressing in a recombinant host cell a wherein said expressing DNA vector contains a recombinant DNA molecule encoding oligopeptide or polypeptide, containing multiple direct repeats of said dipeptide Alo-Pro; b) introducing of said host cells under conditions needed for expressin ofsaid oligopeptide or polypeptide; c) cleaving of the expressed oligopeptide or polypeptide by an enzyme, capable to isolate said dipeptides Ala-Pro from one another to isolate said dipeptides Ala-Pro; and c) separating and purifying said dipeptides Ala-Pro. 2. The process of claim 1, wherein said oligopeptide or polypeptide contain about from 14 to 20 direct repeats Ala-Pro. 3. The process of claim 1, wherein said enzyme is selected from the group containing propylpeptidase Lactobacillus, Xanthomonas maltophilia, Flavorbacterium meningosepticum or mixtures thereof. 4. The process of claim 1, wherein said expressing DNA sector expresses oligopeptide Ala-Pro-Ala-Pro-Ala-Pro. 5. The process of claim 4, wherein said enzyme is selected from the group containing propylpeptidase Lactobacillus, Xanthomonas maltophilia, Flavorbacterium meningosepticum or mixtures thereof. 6. The process of claim 1, wherein said expressing DNA vector encodes fused protein, containing a cleaving fragment, connected to said oligopeptide or polypeptide, containing multiple direct repeatsdipeptide Ala-Pro. 7. The process of claim 6, wherein said cleaving fragment is a domain binding maltose. 8. The process of claim 6, wherein said oligopeptide or polypeptide contain about from 14 to 4 direct repeats Ala-Pro. 9. The process of claim 6 , wherein said expressing DNA sector expresses oligopeptide Ala-Pro-Ala-Pro-Ala-Pro. 10. The process of claim 7, wherein said oligopeptide or polypeptide contain about from 14 to 20 direct repeats Ala-Pro. 11. The process of claim 7, wherein said expressing DNA sector expresses oligopeptide Ala-Pro-Ala-Pro-Ala-Pro. 12. The process of claim 6, wherein said enzyme is selected from the group containing propylpeptidase Lactobacillus, Xanthomonas maltophilia, Flavorbacterium meningosepticum or mixtures thereof. 13. A process for producing a dipeptide Lys-Pro, representing a) expressing in a recombinant host cell a wherein said expressing DNA vector contains a recombinant DNA molecule encoding oligopeptide or polypeptide, containing multiple direct repeats of said dipeptide Lys-Pro; b) introducing of said host cells under conditions needed for expressin ofsaid oligopeptide or polypeptide; c) cleaving of the expressed oligopeptide or polypeptide by an enzyme, capable to isolate said dipeptides Lys-Pro from one another to isolate said dipeptides Lys-Pro; and c) separating and purifying said dipeptides Lys-Pro. 14. The process of claim 13, wherein said expressing DNA sector expresses oligopeptide Lys-Pro-Lys-Pro. 15. The process of claim 14, wherein said enzyme is selected from the group containing propylpeptidase Lactobacillus, Xanthomonas maltophilia, Flavorbacterium meningosepticum or mixtures thereof. 16. The process of claim 1, wherein said expressing DNA vector encodes fused protein, containing a cleaving fragment, connected to said oligopeptide or polypeptide, containing multiple direct repeatsdipeptide Lys-Pro. 17. The process of claim 16, wherein said expressing DNA sector expresses oligopeptide Lys-Pro-Lys-Pro. 18. The process of claim 17, wherein said enzyme is selected from the group containing propylpeptidase Lactobacillus, Xanthomonas maltophilia, Flavorbacterium meningosepticum or mixtures thereof.