Patent SG-11201909618T-A (SG)
Title: Compositions and methods for library construction and sequence analysis
Inventor: GOLE JEFFREY
IPC: C12Q1/6844
Filed: 2018-04-18
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 25 October 2018 (25.10.2018) WIP0 I PCT Iiiimmoimiolollmonolomomuliololommovoiml (10) International Publication Number WO 2018/195217 Al 0 ° Dephosphorylation ° Denaturation (51) International Patent Classification: C12Q 1/68 (2018.01) C12Q 1/6853 (2018.01) C12Q 1/6806 (2018.01) C12Q 1/6855 (2018.01) C12Q 1/6844 (2018.01) C4OB 50/14 (2006.01) (21) International Application Number: PCT/US2018/028191 (22) International Filing Date: 18 April 2018 (18.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/487,423 19 April 2017 (19.04.2017) US 62/657,544 13 April 2018 (13.04.2018) US (71) Applicant: SINGLERA GENOMICS, INC. [US/US]; 505 Coast Blvd S, Ste 307, La Jolla, CA 92037 (US). (72) Inventors: GOLE, Jeffrey, A.; 12362 Carmel Country Rd., Unit G207, San Diego, CA 92130 (US). GORE, Athur- va; 12639 El Camino Real, Apartment 6404, San Diego, CA 92130 (US). LIU, Rui; 50 Guanglan Rd., Ste. 12-101, Shanghai, 201203 (CN). (74) Agent: CHEN, Peng et al.; Rimon, P.C., One Embarcadero Center, Suite 400, San Francisco, CA 94111 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. = (54) Title: COMPOSITIONS AND METHODS FOR LIBRARY CONSTRUCTION AND SEQUENCE ANALYSIS ssDNA Ligation Purification Sequencing 2nd Strand Synthesis Purification Index PCR Purification Semi Targeted PCR FIG.1 (57) : The present disclosure relates to methods for constructing polynucleotide libraries and/or polynucleotide sequencing. Related kits and devices are also disclosed. The present disclosure also relates to compositions, kits, devices, and methods for conduct- ing genetic and genomic analysis, for example, by polynucleotide sequencing. In particular aspects, provided herein are compositions, kits, and methods for constructing libraries with improved ligation efficiency and conversion rate during sequencing. In certain embod- iments, the compositions, kits, and methods herein are useful for analyzing polynucleotide fragments, such as circulating polynucleotide fragments in the body of a subject, Including circulating tumor DNA [Continued on next page] WO 2018/195217 Al MIDEDIMOMOIDEIREEMOMOHHEIHMEnin (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))