Patent DE-102013200822-A1 (DE)
Title: Parallel implementation of number of bio-experiments to identify optimal process parameters, comprises e.g. providing disposable bioreactors, setting first and second value, determining parameter vectors and determining result value
Inventor: SCHNEIDER FALK
IPC: C12M1/36
Filed: 2013-01-18
Abstract: Parallel implementation of a number of bio-experiments to identify optimal process parameters in a simultaneous minimization of a number of bio-experiments, comprises: e.g. providing a number of disposable bioreactors (102); setting a first value (104) and a second value; determining parameter vectors (106); assigning (110) each specific parameter vector from reduced parameter vector to each group of disposable bioreactors; providing the parameter vector component values in a control experiment unit; filling disposable bioreactors with raw materials; and determining a result value. Parallel implementation of a number of bio-experiments to identify optimal process parameters in a simultaneous minimization of a number of bio-experiments, comprises: providing a number of disposable bioreactors (102); setting a first value (104) and a second value for each one of parameters for performing the respective bio-experiments in disposable bioreactors; determining parameter vectors (106) whose components are respectively the predetermined first values and second values such that combinations of each of the first values and second values are respectively provided in a thus formed vectors of parameters, and the set of parameter vectors is a parameter vector group; reducing the number of parameter vectors (108) of the parameter vector set based on a comparison of each specific parameter vector with existing specific parameter vectors for each of which a measurement result of a system based on the specific parameter vector equivalent to bio-experiments exists, such that a reduced parameter vector group is formed from the total parameter vectors; assigning (110) each specific parameter vector from the reduced parameter vector to each group of disposable bioreactors; providing the parameter vector component values in a control experiment unit; filling of disposable bioreactors with raw materials for the bio-experiments; controlling by the experiment control unit which is specific for each disposable bioreactor values of the parameter vectors by detectors on each disposable bioreactor and actuators for the values of the parameter vectors; measuring the disposable bioreactor-specific measurement results of the bio-experiments; and determining a result value of the components of a specific parameter vector based on the measured bioreactor specific measurement results and the corresponding specific parameter vector values, and the existing specific parameter vectors and associated measurement results, such that a minimum number of experiments are needed for the determination of optimal specific process parameters. Independent claims are also included for: (1) a bio-experiment control system for parallel implementation of a number of organic experiments in many disposable bioreactors for the determination of optimal process parameters in a simultaneous minimization of the number of bio-experiments, comprising an interface component for receiving a first value and a second value, a combination module which is adapted for determination of specific parameter vectors whose components are respectively the predetermined first values and second values, reduction module that is adapted for reducing the number of vectors of parameters of the parameter vector group, an assignment module adapted to assign a respective specific parameter vector from the reduced parameter vector group to disposable bioreactor, a transmission module, which is adapted to provide the parameter vector component values based on results of the allocation module to a experiment control unit, a control module adapted to control the specific values for each disposable bioreactor of parameter vectors by the experiment control unit, a measurement module that is adapted for measuring disposable bioreactor-specific measurement results of the bio-experiments, and a calculation module adapted for determining a result value of the components of a specific parameter vector based on the measured bioreactor specific measurement results and the corresponding specific parameter vector values, and the existing parameter vectors and associated measurement results; (2) a computer system comprising the bio-experiment control system (400) for parallel implementation of a number of bio-experiments; and (3) a data processing program for controlling a parallel implementation of a number of bio-experiments, comprising software code portions capable of implementing the above method when the computer program is run on a data processing system; and (4) a computer program product for controlling a parallel implementation of bio-experiments stored on a computer-readable medium, comprising computer-processable program means for causing a computer to perform the above method when the program means are executed on the computer.