Patent SG-11201907148S-A (SG)
Title: Two-dimensional amorphous carbon coating and methods of growing and differentiating stem cells
Inventor: ÖZYILMAZ BARBAROS
IPC: C23C16/26
Filed: 2018-02-23
Abstract: 102 104 00 O 00 O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 30 August 2018 (30.08.2018) WIP0 I PCT ill mu °million °nolo moll imio oimIE (10) International Publication Number WO 2018/156082 Al (51) International Patent Classification: C01B 32/15 (2017.01) A61L 31/08 (2006.01) C23C 16/26 (2006.01) C12N 5/07 (2010.01) (21) International Application Number: PCT/SG2018/050082 (22) International Filing Date: 23 February 2018 (23.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/463,112 24 February 2017 (24.02.2017) US 62/546,680 17 August 2017 (17.08.2017) US (71) Applicant: NATIONAL UNIVERSITY OF SINGA- PORE [SG/SG]; 21 Lower Kent Ridge Road, Singapore 119077 (SG). (72) Inventors: OZYILMAZ, Barbaros; c/o National Uni- versity of Singapore, Faculty of Science, Department of Physics, 21 Lower Kent Ridge Road, Singapore 119077 (SG). OROFEO, Carlo Mendoza; c/o National University of Singapore, Faculty of Science, Department of Physics, 21 Lower Kent Ridge Road, Singapore 119077 (SG). AN- DERSEN, Henrik; c/o National University of Singapore, Faculty of Science, Department of Physics, 21 Lower Kent Ridge Road, Singapore 119077 (SG). ZHANG, Hongji; c/o National University of Singapore, Faculty of Science, Department of Physics, 21 Lower Kent Ridge Road, Sin- gapore 119077 (SG). TOH, Chee Tat; c/o National Uni- versity of Singapore, Faculty of Science, Department of Physics, 21 Lower Kent Ridge Road, Singapore 119077 (SG). MARTIN-FERNANDEZ, Inigo; c/o National Uni- versity of Singapore, Faculty of Science, Department of Physics, 21 Lower Kent Ridge Road, Singapore 119077 (SG). (54) Title: TWO-DIMENSIONAL AMORPHOUS CARBON COATING AND METHODS OF GROWING AND DIFFEREN- TIATING STEM CELLS FIG. (57) : Described is a composite material composed of an atomically thin (single layer) amorphous carbon disposed on top of a substrate (metal, glass, oxides); an article formed thereof and method of forming the article, comprising decomposing a carbon-con- taining gas (i.e. methane) to generate at least one decomposed species; and forming the 2D amorphous carbon (2DAC) film on the surface of the substrate. The 2DAC film can be used as a coating having an atomic structure consisting of non-hexagonal and hexagonal carbon rings, having a ratio of the hexagonal carbon rings to the non -hexagonal carbon rings is less than 1.0, wherein the coating is an implant coating. The present disclosure also provides a method of growing and differentiating stem cells, comprising disposing a seed layer on the surface of the substrate coated with 2DAC; and absorbing growth factors in a stem cell medium onto the surface of the substrate coated with 2DAC. [Continued on next page] WO 2018/156082 Al MIDEDIMOMOIDEIREEMOMOMMIIMMEIBUDIMIE (74) Agent: AMICA LAW LLC; 30 Raffles Place, #14-01 Chevron House, Singapore 048622 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE