Patent EA-003832-B1 (EA)
Title: Gene delivery complex for percutaneous delivery of foreign genetic material in an antigen presenting cells and method for use
Inventor: LISZIEWICZ JULIANNA
IPC: A61K31/17
Filed: 1998-09-15
Abstract: 1. A gene delivery complex for percutaneous delivery of foreign genetic material in an antigen presenting cells, comprising the foreign genetic material and a non-viral vector, wherein the vector having specific affinity for the foreign genetic material and a receptor on an antigen presenting cell. 2. The gene delivery complex of Claim 1, wherein the foreign genetic material is a DNA molecule which encodes a reverse-transcriptase dependent virus or a mutant reverse-transcriptase dependent virus. 3. The gene delivery complex of Claim 2, wherein the foreign genetic material is a DNA molecule encoding at least a substantial portion of a replication-defective human immunodeficiency virus. 4. The gene delivery complex of Claim 3, wherein the foreign genetic material is a DNA molecule at least a substantial portion of an integrase negative mutant of a dual-tropic primary isolate of a human immunodeficiency virus. 5. The gene delivery complex of Claim 4, wherein the DNA further includes one or more stop codons in one or more of the reading frames of the integrase gene. 6. The gene delivery complex of Claim 1, wherein the complex is selected from the group consisting of DNA conjugates of sugars, polyethylenimine, polyethylenimine derivatives, and mixtures thereof. 7. The gene delivery complex of Claim 1, wherein the antigen presenting cell is a Langerhans cell or a dendritic cell. 8. The gene delivery complex of Claim 1, wherein the receptor on an antigen presenting cell is the mannose receptor. 9. Method of transducing cells with foreign genetic material comprising the steps of exposing the cells to the gene delivery complex described in any one of Claims 1 - 8. 10. Method of producing a foreign gene product in vitro, wherein comprising: (a) transducing an antigen presenting target cell of Claim 9; and (b) cultivating said transduced antigen presenting target cell under conditions suitable for expressing aim foreign gene product. 11. Method of preventive and therapeutic immunization for animals by exposing the animal to a gene delivery complex described in any one of Claims 1-8. 12. Method of preventive and therapeutic immunization for animals against reverse-transcriptase-dependent virus infection, the steps comprising: isolating dendritic cells from the animal, exposing the cells to the gene delivery complex described in any one of Claims 1 -8, and reintroducing the cells to the body of the animal. 13. Method of preventive and therapeutic immunization for animals against reverse-transcriptase-dependent virus infection, the steps comprising exposing the animal to the gene delivery complex described in any one of Claims 1 -8 on the skin or on mucosa surfaces. 14. Method of treating active virus infection, such as an HIV infection, wherein comprising the steps of: treating a patient in need thereof with a drug combination suitable for suppressing viral load, immunizing the patient with the gene delivery complex of any one of Claims 1 to 8 and continuing treatment with a drug combination suitable for suppressing viral load until immune response develops, and monitoring for rebound of the patient's viral load; and in the event viral load rebounds, restarting treatment with the drug combination, or another drug combination suitable for suppressing viral load and immunizing the patient with the gene delivery complex of any one of Claims 1 to 8.