Patent SG-11201911919P-A (SG)
Title: Image capillary isoelectric focusing to analyze protein variants in a sample matrix
Inventor: RAMBHADRAN ANU
IPC: G01N27/27
Filed: 2018-08-17
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) CORRECTED VERSION (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11111 1011111DEllIIIII11111N 1111111111 11111 11111 11111 11111 11111 1111 MINN 11111111 (10) International Publication Number WO 2019/036604 A9 21 February 2019 (21.02.2019) WI PO I PCT (51) International Patent Classification: A61K 39/395 (2006.01) A61K 39/44 (2006.01) GO1N 27/26 (2006.01) GO1N 27/27 (2006.01) GO1N 33/15 (2006.01) (21) International Application Number: PCT/US2018/046893 (22) International Filing Date: 17 August 2018 (17.08.2018) English English Inc., 777 Old Saw Mill River Rd., Tarrytown, NY 10591 (US). LU, Kun; c/o Regeneron Pharmaceuticals, Inc., 777 Old Saw Mill River Rd., Tarrytown, NY 10591 (US). (74) Agent: PAVAO, Matthew; Cooley LLP, 1299 Pennsylva- nia Avenue, NW, Suite 700, Washington, DC 20004-2400 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, 1E, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/547,602 18 August 2017 (18.08.2017) US (71) Applicant: REGENERON PHARMACEUTICALS, INC. [US/US]; 777 Old Saw Mill River Rd., Tarrytown, NY 10591 (US). (72) Inventors: RAMBHADRAN, Anu; c/o Regeneron Phar- maceuticals, Inc., 777 Old Saw Mill River Rd., Tarrytown, NY 10591 (US). CUI, Peng; c/o Regeneron Pharmaceu- ticals, Inc., 777 Old Saw Mill River Rd., Tarrytown, NY 10591 (US). RYAN, Clare; c/o Regeneron Pharmaceuti- cals, Inc., 777 Old Saw Mill River Rd., Tarrytown, NY 10591 (US). BIGWARFE, Paul; c/o Regeneron Pharma- ceuticals, Inc., 777 Old Saw Mill River Rd., Tarrytown, NY 10591 (US). LASTRO, Michele; c/o Regeneron Pharma- ceuticals, Inc., 777 Old Saw Mill River Rd., Tarrytown, NY 10591 (US). MA, Junyu; c/o Regeneron Pharmaceuticals, (54) Title: IMAGE CAPILLARY ISOELECTRIC FOCUSING TO ANALYZE PROTEIN VARIANTS IN A SAMPLE MATRIX Figure IA 0.24- 0.22- 0.20- 0.18- 0.16- 0 014- 011- a FA- CT ' c 0.06- OSA- 002- 000- Nre -0.02 3. , , 0 120 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6t 610 640 6.60 080 7.00 7.20 740 700 7.80 8.00 . 8.10 8.40 . . 8.008.80 ( 9.009.209.409.60 . 6.80 Tao en O 1-1 O (57) : Embodiments of the present disclosure are directed to methods, systems, devices and kits corresponding to a method CN1I for analyzing charge variants of a protein such as vascular endothelial growth factor VEGF-Trap. C [Continued on next page] WO 2019/036604 A9 I ill Hilt II UI OH 11111 DM MI I II III ill Di Declarations under Rule 4.17: — as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(h)) Published: with international search report (Art 21(3)) with sequence listing part of description (Rule 5.2(a)) (48) Date of publication of this corrected version: 04 April 2019 (04.04.2019) (15) Information about Correction: see Notice of 04 April 2019 (04.04.2019)