Patent SG-11201407405V-A (SG)
Title: Method for identifying ligands of the ahr receptor having therapeutic sebosuppressive activity, and said ligands
Inventor: SAURAT JEAN HILAIRE
IPC: G01N33/68
Filed: 2013-05-15
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 21 November 2013 (21.11.2013) WIPOIPCT (10) International Publication Number WO 2013/171696 A1 (51) International Patent Classification: G01N33/50 (2006.01) G01N33/68 (2006.01) (21) International Application Number: PCT/IB2013/053979 (22) International Filing Date: 15 May 2013 (15.05.2013) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 12168121.7 15 May 2012 (15.05.2012) EP (72) Inventor; and (71) Applicant SAURAT, : Jean, Hilaire [FR/CH]; 22 rue de l'Athenee, CH-1206 Geneve (CH). (74) Agents: BUGNION GENEVE et al.; 10, route de Floris­ sant, Case Postale 375, CH-1211 Geneva 12 (CH). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) (54) Title: METHOD FOR IDENTIFYING LIGANDS OF THE AHR RECEPTOR HAVING THERAPEUTIC SEBOSUPPRESS- IVE ACTIVITY, AND SAID LIGANDS Fig.2 Progenitor cells 1 B Undifferentiated cells 28 \V St- (57) Abstract: A method for sorting substances for the purpose of determin­ ing their capacity for sebosuppressive activity in topical skin treatment, com­ prising an in vivo test, the said in vivo test comprising the following steps: -choosing substance from a among the ligands of the AhR receptor; -choos­ ing a mammal expressing the CYP1A1 gene; -treating via topical route part of the skin of the said mammal comprising sebaceous glands, with a com­ position comprising the said substance, following a dose/ response/response time protocol; -examining by immunohistochemical staining the expression of CYP1A in sebaceous glands of the said part of the skin of the said mam­ mal; -selecting the said substance as a function of the sequence of onset of immunohistochemical staining in several different types of cells of the said sebaceous glands. Pharmaceutical composition for topical use intended to re­ duce sebogenesis, characterized in that contains it 5,6 benzoflavone as active substance. Differentiated cells 3B \" Mature ceils 4B