Patent SG-11201803878W-A (SG)
Title: Engineering structurally defined non-saccharide glycosaminoglycan mimetics via a polyproline scaffold
Inventor: LEE SONG-GIL
IPC: C12P13/24
Filed: 2016-11-11
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property - Organization International Bureau (43) International Publication Date ..... ...sre.1 18 May 2017 (18.05.2017) WIPO I PCT (10) WO International Publication Number 111111111111311111111111111111111111111111111111111111111111111111111111111111111111111111 2017/082827 Al (51) International Patent Classification: 138669 (SG). LEE, Su Seong; c/o Institute of Bioengin- C07K 14/00 (2006.01) A61P 25/00 (2006.01) eering and Nanotechnology, 31 Biopolis Way, The Nanos, C12P 13/24 (2006.01) A61P 35/00 (2006.01) #04-01, Singapore 138669 (SG). KHOU, Siv Ly; do Insti- A61K 31/401 (2006.01) tute of Bioengineering and Nanotechnology, 31 Biopolis (21) International Application Number: Way, The Nanos, #04-01, Singapore 138669 (SG). TOH, Kah Chin Jerry; c/o Institute of Bioengineering and Nan- PCT/SG2016/050559 otechnology, 31 Biopolis Way, The Nanos, #04-01, Singa- (22) International Filing Date: pore 138669 (SG). JEE, Joo-Eun; c/o Institute of Bioen- 11 November 2016 (11.11.2016) gineering and Nanotechnology, 31 Biopolis Way, The Nanos, #04-01, Singapore 138669 (SG). (25) Filing Language: English (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; (26) Publication Language: English P.O. Box 1531, Robinson Road Post Office, Singapore (30) Priority Data: 903031 (SG). 10201509331Y 12 November 2015 (12.11.2015) SG (81) Designated States (unless otherwise indicated, for every 10201602104X 17 March 2016 (17.03.2016) SG kind of national protection available): AE, AG, AL, AM, (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, Connexis, Singapore 138632 (SG). DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (72) Inventors: LEE, Song-Gil; c/o Institute of Bioengineering KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, and Nanotechnology, 31 Biopolis Way, The Nanos, #04- MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, 01, Singapore 138669 (SG). LIM, Teck Chuan; c/o Insti- OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, tute Bioengineering Nanotechnology, 31 Biopolis of and SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, Way, The Nanos, #04-01, Singapore 138669 (SG). CAI, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, Shuting; c/o Institute of Bioengineering and Nanotechno- ZW. logy, 31 Biopolis Way, The Nanos, #04-01, Singapore [Continued on next page] (54) Title: ENGINEERING STRUCTURALLY DEFINED NON-SACCHARIDE GLYCOSAMINOGLYCAN MIMETICS VIA A POLYPROLINE SCAFFOLD : A A (57) non-saccharide glycosaminoglycan mimetic molecule com- prising a polyproline backbone and one or more non- saccharide molecules, k OH ' 0 2$0 wherein the non-saccharide molecules are bound to one or more prolines Ec o_,..--- o,so„),, , ' and/or proline derivatives the backbone, is disclosed herein. In on polyproline 1 2 4 NS a preferred embodiment the non-saccharide molecule is a negatively charged sulfate. The invention also relates to methods of synthesizing said non-sac - charide glycosaminoglycan mimetic molecule and the use of the molecules B thereof in targeting cell adhesion molecules such as selectin. '‘P I l MINIS 0114 ---• 0112404 I 0 r ` \ -` X‘ R •1^ - 0 1- t3 Q C7 6 6 R = OPPE4u myl H I'l 0 N 0 1 , 4 141 .4t S' t ' ' -' - '1 , -\ Blottn ° IN ei ot ot ei C IN I'l C ei N II P - 0P - -- p Fig. 9 -0190 , -* 0` i pild4 GMs * O WO 2017/082827 Al MIDEDIMOHIMIRDIDENEHHEINERUDEN (84) Designated States (unless otherwise indicated, for every Declarations under Rule 4.17: kind of regional protection available): ARIPO (BW, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, GH, SZ, RU, of inventorship (Rule 4.17(iv)) Published: TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DK EE ES FI FR GB GR HR HU IE IS IT , , , , , , , , , , , , LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, DE, LT , SE, GA, with international search report (Art 21(3)) GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).