Patent SG-11201900518X-A (SG)
Title: Biomarker detection system
Inventor: SUN LING LING
IPC: G01N21/64
Filed: 2017-07-21
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 25 January 2018 (25.01.2018) WIP0 I PCT omit °nolo omm olo (10) International Publication Number WO 2018/017021 A3 (51) International Patent Classification: GO1N 21/64 (2006.01) GO1N 21/552 (2014.01) (21) International Application Number: PCT/SG2017/050372 (22) International Filing Date: 21 July 2017 (21.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201606001Q 21 July 2016 (21.07.2016) SG (71) Applicants: TEMASEK POLYTECHNIC [SG/SG]; 21 Tampines Avenue 1, Singapore 529757 (SG). AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10, Connexis, Singa- pore 138632 (SG). TAN TOCK SENG HOSPITAL PTE LTD [SG/SG]; 11 Jalan Tan Tock Seng, Singapore 308433 (SG). (72) Inventors: SUN, Ling Ling; c/o Temasek Polytechnic, 21 Tampines Avenue 1, Singapore 529757 (SG). NG, Willie; c/o Temasek Polytechnic, 21 Tampines Avenue 1, Sin- gapore 529757 (SG). ZHOU, Xiaodong; c/o Institute of Materials Research and Engineering 2 Fusionopolis Way, #08-03, Innovis, Singapore 138634 (SG). WONG, Ten It; c/o Institute of Materials Research and Engineering 2 Fu- sionopolis Way, #08-03, Innovis, Singapore 138634 (SG). LEO, Yee Sin; c/o Tan Tock Seng Hospital Pte Ltd, 11 Jalan Tan Tock Seng, Singapore 308433 (SG). (74) Agent: DAVIES COLLISON CAVE ASIA PTE. LTD.; 10 Collyer Quay #07-01 Ocean Financial Centre, Singapore 049315 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (54) Title: BIOMARKER DETECTION SYSTEM Layer 1 PMMA Layer 2 Double-side adhesive Mylar ® Layer 3 Single-side adhesive Mylar ® Layer 4 PMMA O N O 00 O O Figure 5 (57) : Disclosed is a localised surface plasmon resonance (LSPR) nanopillar assembly. The LSPR assembly is for use in sensing the presence of a biomarker when attached to a quantum dot. The LSPR assembly comprises a substrate and an array. The array comprises a LSPR nanopillar and a polymer spacer attached to the nanopillar. The LSPR assembly further comprises an antibody attached to the at least one polymer spacer. In the LSPR assembly, a combined height of the polymer spacer and antibody is selected by varying the number of monomer units of the polymer spacer, so that, when in use with the biomarker and the quantum dot, the quantum dot is at a predetermined distance from the nanopillar. [Continued on next page] WO 2018/017021 A3 MIDEDIMOMOIDEIRDERIO 011111101011011010111 11111111101110 II MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) (88) Date of publication of the international search report: 21 June 2018 (21.06.2018)