Patent SG-11201903042V-A (SG)
Title: Engineered meganucleases specific for recognition sequences in the hepatitis b virus genome
Inventor: JANTZ DEREK
IPC: C12N9/22
Filed: 2017-10-13
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property MillEIMO REE N EVIEHEDERIMEEIIMMEEMIE Organization - ' (10) International Publication Number International Bureau (43) International Publication Date .....0\"\" WO 2018/071849 A2 19 April 2018 (19.04.2018) WIP0 1 PCT _ (51) (21) (22) (25) Filing Language: (26) (30) (71) International Patent Classification: (72) Inventors: JANTZ, C12N 9/22 (2006.01) A61K 38/00 (2006.01) NC 27701 (US). Forest Way, Morrisville, NC 27560 (US). International Application Number: PCT/US2017/056638 (74) Agent: DIPIETRANTONIO, International Filing Date: field & Sacks, 02210-2206 (US). 13 October 2017 (13.10.2017) English (81) Designated States kind of national Publication Language: English AO, AT, AU, AZ, Priority Data: CA, CH, CL, CN, CO, CR, CU, CZ, DE, 62/408,356 14 October 2016 (14.10.2016) US DZ, EC, EE, EG, ES, FF GB, GD, GE, 62/452,506 31 January 2017 (31.01.2017) US HR, HU, ID, IL, 62/527,159 30 June 2017 (30.06.2017) US KR, KW, KZ, LA, 62/527,196 30 June 2017 (30.06.2017) US MG, MK, MN, OM, PA, PE, PG, PH, PL, PT, QA, RO, Applicant: PRECISION BIOSCIENCES, INC. SC, SD, SE, SG, [US/US]; 302 E. Pettigrew Street, Dibrell Building, Suite TR, TT, TZ, UA, A-100, Durham, NC 27701 (US). (84) Designated States kind of regional GM, KE, LR, LS, Derek; 1011 Gloria Avenue, Durham, 1916 Weaver J.; Wolf, Green- Boston, MA for every AE, AG, AL, AM, BR, BW, BY, BZ, DJ, DK, DM, DO, GH, GM, GT, HN, KG, KH, KN, KP, LY, MA, MD, ME, NG, NI, NO, NZ, RS, RU, RW, SA, TH, TJ, TM, TN, ZA, ZM, ZW. for every ARIPO (BW, GH, SL, ST, SZ, TZ, SMITH, James, Jefferson; Heather, P.C., 600 Atlantic Avenue, (unless otherwise indicated, protection available): BA, BB, BG, BH, BN, IN, IR, IS, JO, JP, KE, LC, LK, LR, LS, LU, MW, MX, MY, MZ, NA, SK, SL, SM, ST, SV, SY, UG, US, UZ, VC, VN, (unless otherwise indicated, protection available): MW, MZ, NA, RW, SD, = Title: ENGINEERED : The an open reading methods symptoms of a HBV infection, or treating hepatocellular carcinoma (HCC). Further, the invention encompasses (54) GENOME ---- encompasses 0 compositions N compositions for treating HBV infections or HCC. MEGANUCLEASES SPECIFIC FOR RECOGNITION SEQUENCES IN THE HEPATITIS FIGURE 5 First Subunit Second Subunit B VIRUS I recognition sequence present invention also treating or reducing pharmaceutical and the use of such = EE = EE = = I I 6 = M. iail i: \"i \V ::;::::;::;:::: = HBV1 Second Subunit Linker HBV2 First Subunit i = = = -- M . .M. ei -4! CT 71' GO 1-1 (57) Ir----- within 0 GO , 1 the comprising HBV2 Linker HBV1 present invention encompasses engineered meganucleases which recognize and cleave a frame (ORF) of the genome of at least two genotypes of the Hepatitis B virus (HBV). The of using such engineered meganucleases in a pharmaceutical composition and in methods for engineered meganuclease proteins, nucleic acids encoding engineered meganucleases, C [Continued on next page] WO 2018/071849 A2 IMIOHOIIIMIIMEIHOMMOHOIRIONMOIME UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — without international search report and to be republished upon receipt of that report (Rule 48.2(g)) — with sequence listing part of description (Rule 5.2(a))