Patent SG-11201900753V-A (SG)
Title: A method of isolating mesenchymal stem cells from umbilical cord amniotic membrane using a cell culture medium
Inventor: PHAN TOAN THANG
IPC: C12N5/0775
Filed: 2017-10-05
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 12 April 2018 (12.04.2018) WIP0 1 PCT °mons °nolo omoiloo mon ilommio oimIE (10) International Publication Number WO 2018/067071 Al (51) International Patent Classification: C12N 5/0775 (2010.01) A61K 35/51 (2015.01) (21) International Application Number: PCT/SG2017/050500 (22) International Filing Date: 05 October 2017 (05.10.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/404,582 05 October 2016 (05.10.2016) US (71) Applicant: CELLRESEARCH CORPORATION PTE. LTD. [SG/SG]; 137 Market Street, #08-02, Grace Global Raffles Building, Singapore 048943 (SG). (72) Inventor: PHAN, Toan Thang; 137 Market Street, #08-02, Grace Global Raffles Building, Singapore 048943 (SG). (74) Agent: MCLAUGHLIN, Michael Gerard; McLaughlin IP Pte. Ltd., 24A Mosque Street, Singapore 059504 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, = (54) Title: A METHOD OF ISOLATING MESENCHYMAL STEM CELLS FROM UMBILICAL CORD AMNIOTIC MEMBRANE USING A CELL CULTURE MEDIUM 99,70% 99,80% 139,50% 99,00% 99,70% 99,50% 1-1 1-1 N O N O GC O 1-1 O Fig. 6c (57) : The present invention relates to a method of isolating a mesenchymal stem cell population from the amniotic membrane of the umbilical cord, the method comprising cultivating umbilical cord tissue in a culture medium comprising DMEM (Dulbecco's modified eagle medium), F12 (Ham's F12 Medium), M171 (Medium 171) and FBS (Fetal Bovine Serum). The invention also relates to a mesenchymal stem population isolated from the amniotic membrane of the umbilical cord, wherein at least about 90 % or more cells of the stem cell population express each of the following markers: CD73, CD90 and CD105 and lack expression of the following markers: CD34, CD45 and HLA-DR. The invention also relates to a pharmaceutical composition of this mesenchymal stem population. [Continued on next page] WO 2018/067071 Al MIDEDIMOMOIDEIROHOINII01100111011EIHIEMOVOIS MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h))