Patent SG-11201809048P-A (SG)
Title: Improved method for producing virus like particles
Inventor: CARDOSA MARY JANE
IPC: C12N15/57
Filed: 2017-04-19
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#11101111011101010111110101111101110111110ifill10110411011101111 Organization International Bureau (10) International Publication Number (43) International Publication Date .......'\" WO 2017/184696 Al 26 October 2017 (26.10.2017) WIPO I PCT (51) International Patent Classification: HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, A61K 39/12 (2006.01) C12N 15/57 (2006.01) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, C12N 15/40 (2006.01) MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, (21) International Application Number: PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, PCT/US2017/028300 SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (22) International Filing Date: (84) Designated States (unless otherwise indicated, for every 19 April 2017 (19.04.2017) kind of regional protection available): ARIPO (BW, GH, (25) Filing Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (26) Publication Language: English UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (30) Priority Data: EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, 62/326,129 22 April 2016 (22.04.2016) US MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, (71) Applicant: INTEGRATED RESEARCH KM, ML, MR, NE, SN, TD, TG). ASSOCIATES, LLC [US/US]; 4050 Redwood Highway, Suite E, San Rafael, California 94903 (US). Published: (72) Inventors: CARDOSA, Mary Jane; 47-2-2 Gurney — with international search report (Art. 21(3)) ville, Jalan Pemenang, Georgetown, Penang, 10250 (MY). — with sequence listing part of description (Rule 5.2(a)) _ BOSSART, Katharine; 178 Commonwealth Ave., San Francisco, California 94118 (US). — = Agent: PELLEGRINI, Carl J. et al.; SUGHRUE MION (74) PLLC, 2100 Pennsylvania Avenue, Suite 800, Washington, = District of Columbia 20037 (US). _ = Designated States (unless otherwise indicated, for every (81) = kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, Title: IMPROVED METHOD FOR PRODUCING VIRUS LIKE PARTICLES = (54) = — FIGURE 9 = = = = RSV tk polyA signal p10 AddlilPV $V40 pulyA = = _ 0 doIC Flash der FIW = FtigNi NS1 BMA getsi HIM E IRES hfsp iluman ftwin voprpteln 1-1 0\ 0 (57) : The present invention relates to the production of structural proteins and virus-like particles (VLPs) of flaviviruses and 71' of hepatitis C. Production is through use of a single expression cassette comprising a viral structural gene, a furin encoding gene and a 1 -1 bicistronic expression element such as an internal ribosome entry site (IRES) between the viral and furin genes. Both the viral gene and furin gene can include a partial capsid encoding sequence acting as a signal peptide to co-locate the viral protein and furin and to Il act as a membrane anchor for the viral protein and furin. A separate expression cassette comprising a non-structural viral gene can be combined with the initial cassette. The structural proteins and VLPs can be used in vaccines and in the treatment of viral infections. ei C