Patent EP-2505636-A1 (EP)
Inventor: LEGRAND CATHERINE
IPC: C12R1/89
Filed: 2011-04-01
Abstract: The invention relates to a method for recovering a specific cellular component from a microorganism, which comprises steps of: providing a biomass of a microorganism, adding algal cytotoxin or a chemical analogue or derivative thereof, which is capable of rupturing the cell wall and / or cell membrane of the microorganism and also cell wall and/or cell membrane of at least one of the following: 
 
microalgae selected from the group consisting of species of the genera  Achnantes, Agmenellum,Amphiprora, Amphora, Anabaena, Anabaenopsis, Ankistrodesmus, Arthrospira, Attheya, Boeklovia, Botryococcus, Biddulphia, Brachiomonas, Bracteococcus, Carteria, Chaetoceros, Characium, Chlamydomonas, Cricophaera, Crypthecodinium, Cryptomonas, Chlorella, Chlorococcum, Chrysophaera, Coccochloris, Cocconeis, Cyclotella, Cylindrotheca, Dermocarpa, Dunaliella, Ellipsoidon, Entomoneis, Euglena, Eremosphaera, Extubocellulus, Franceia, Fragilaria, Gleocapsa, Gleothamnion, Hantzschia, Haematococcus, Hormotilopsis, Hymenomonas, Isochrysis, Lepocinclis, Melosira, Minidiscus, Micractinum, Microcystis, Monallanthus, Monoraphidium, Muriellopsis, Nannochloris, Nannochloropsis, Navicula, Neochloris, Nephroselmis, Nitzschia, Nodularia, Nostoc, Ochromonas, Oedogonium, Oocystis, Oscillatoria, Papiliocellulus, Parachlorella, Pascheria, Pavlova, Peridinium, Phaeodactylum, Phagus, Plankthothrix, Platymonas, Plectonema, Pleurochrysis, Phormidium, Pleurosigma, Porphyridium, Prymnesium, Pseudochlorella, Pyramimonas, Pyrobotrus,Radiosphaera, Rhodomonas, Rhodosorus, Sarcinoid, Scenedesmus, Schizochytrium, Scrippsiella, Seminavis, Skeletonema, Spirogyra, Spirulina, Stichococcus, Synechococcus, Synechocystis, Synedra, Tetraedron, Tetraselmis, Thalassiosira, Trachyneis, Traustrochytrium, Trentepholia, Ulkenia, Viridiella, Volvox  and  Xenococcus   
or bacteria selected from the group consisting of the genera  Acinetobacter, Actinobacter, Aerogenes, Alcanivorax, Arthrobacter, Bacillus, Clostridium, Cupriviadus, Dietzia, Gordonia, Escherichia, Flexibacterium, Micrococcus, Mycobacterium, Nocardia, Pseudomonas, Ralstonia, Rhodococcus, Rhodomicrobium, Rhodopseudomonas, Shewanella, Shigella, Streptomyces, Wautersia  and  Vibrio  or yeasts selected from the group consisting of the genera  Candida, Clavispora, Cryptococcus, Deparyomyces, Endomycopsis, Galactomyces, Hansenula, Kluyveromyces, Lipomyces, Pachysolen, Pichia, Rhodosporidium, Rhodotorula, Saccharomyces, Waltomyces, Yarrowia  and  Trichosporon  or 
filamentous fungi selected from the group consisting of the genera  Absidia, Aspergillus, Blakeslea, Chaetomium, Cladosporium, Claviceps, Cladosporidium, Cunninghamella, Emericella, Entomophthora, Fusarium, Gibberella, Glomus, Humicola,  Malbranchea,  Mucor, Mortierella, Paecilomyces, Penicillium, Puccia, Pythium, Rhizopus, Saprolegnia, Trichoderma, Thermomycs, Ustilago  and  Zygorhynchus  to the microbial biomass, 
-releasing an intracellular component from the cell, and 
-recovering said intracellular component. The invention relates also a composition used for rupturing lipid-producing algae cells and an integrated system for rupturing lipid-producing algae cells.