Patent SG-11201900058Y-A (SG)
Title: Method of generating mesenchymal stem cells and uses thereof
Inventor: Liu tong ming
IPC: C12N5/0775
Filed: 2017-07-04
Abstract: Mesoderm FOXF+- Handl+ APLNR1 MSC CD14-, CD34-, CD45-, CO29+, CD44+, C049c+, 0373+, CD105+, CD151+, CD166+ (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11 January 2018 (11.01.2018) WIP0 I PCT onion OH oI0IIIIIIII1101HDIDMDlII111111 DIIIIII (10) International Publication Number WO 2018/009147 Al (51) International Patent Classification: C12N 5/0775 (2010.01) A61P 19/04 (2006.01) A61K 35/28 (2015.01) (21) International Application Number: PCT/SG2017/050339 (22) International Filing Date: 04 July 2017 (04.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201605481T 04 July 2016 (04.07.2016) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 (SG). (72) Inventors: LIU, Tong Ming; c/o Genome Institute of Sin- gapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). LIM, Bing; c/o Genome Institute of Sin- gapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). LI, Pin; c/o Genome Institute of Singapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) = (54) Title: METHOD OF GENERATING MESENCHYMAL STEM CELLS AND USES THEREOF 1-1 N 1-1 t:T O O GC O 1-1 N O ACF FBRFs FPEVc do di d2 Stage 1 d10 Stage 2 d21 Stage 3 (57) : The present disclosure provides a method of generating mature mesenchymal stem cells (MSC) from lateral plate mesoderm comprising culturing the lateral plate mesoderm cells on an extracellular matrix in a MSC cell culture media comprising (i) fibroblast growth factor; (ii) platelet-derived growth factor (PDGF); (iii) epidermal growth factor (EGF) and (iv) ascorbic acid. Also claimed are MSCs obtained by said culture method and a method of treating cartilage and bone disease in a patient using said cells. In addition, a culture medium for deriving primitive streak mesendoderm cells from pluripotent stem cells comprising (a) activin; (b) WNT- signalling activator; and/or (c) fibroblast growth factor and a culture medium for deriving lateral plate mesoderm cells from primitive streak mesendoderm cells comprising (1) fibroblast growth factor; (2) bone morphogenetic protein; (3) follistatin; and optionally Rho- associated protein kinase (ROCK) inhibitor are claimed.