Patent SG-11201810998W-A (SG)
Title: Engineered beta-glucosidases and glucosylation methods
Inventor: LIANG JACK
IPC: C07H21/00
Filed: 2017-06-09
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) - (19) World Intellectual Property 111111101111011101111111101011111001111111111111111111111111011111111 Organization - - ' International Bureau (10) International Publication Number (43) International Publication Date ....0\"\"\" WO 2017/218325 Al 21 December 2017 (21.12.2017) WIP0 I PCT (51) International Patent Classification: (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 A61K 36/00 (2006.01) C07H 21/04 (2006.01) Penobscot Drive, Redwood City, California 94063 (US). C07H 21/00 (2006.01) CO7K 14/195 (2006.01) (81) Designated States (unless otherwise indicated, for every (21) International Application Number: kind of national protection available): AE, AG, AL, AM, PCT/US2017/036704 AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (22) International Filing Date: CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, 09 June 2017 (09.06.2017) DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (25) Filing Language: English KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (26) Publication Language: English MG, MK, MN, MW, MX, MY, MZ, NA, NG, NE NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (30) Priority Data: 62/350,625 15 June 2016 (15.06.2016) US SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- (84) ye, Redwood City, California 94063 (US). Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (72) Inventors: LIANG, Jack; 200 Penobscot Drive, Redwood UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, City, California 94063 (US). VROOM, Jonathan; 200 TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Penobscot Drive, Redwood City, California 94063 (US). EE, ES, FE FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, — GALANIE, Stephanie, Sue; 200 Penobscot Drive, Red MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, wood City, California 94063 (US). DELLAS, Nikki; 200 TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, — Penobscot Drive, Redwood City, California 94063 (US). KM, ML, MR, NE, 5N, TD, TG). = GARCIA, Ravi, David; 200 Penobscot Drive, Redwood City, California 94063 (US). (54) Title: ENGINEERED BETA-GLUCOSIDASES AND GLUCOSYLATION METHODS = = = = = Ho :a' \ = QH OH Ho— = — _ \ e = Cellabiose ot Oth er d1sxchar7tf6 a: , s E! 0 f = Other c:rictsaccharide _ = Fi = A : ,. .,epitur: Acc 9 pLcr 0.-g iLIC 13 Se of other sugar linkace 1-1 kin ei M FIG. 1 GO 1-1 ei ---- (57) : The present invention provides engineered 0-glucosidase (BGL) enzymes, polypeptides having BGL activity, and IN polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The 0 present invention also provides compositions comprising the enzymes, and methods of using the engineered BGL enzymes to make N products with 0-glucose linkages. o [Continued on next page] WO 2017/218325 Al MIDEDIMOMMIDIRMEMIllfillOillIODEVOIS Published: — with international search report (Art. 21(3)) — before the expiration of the time limit for amending the claims and to be republished in the event of receipt amendments (Rule 48.2(h)) of — with sequence listing part of description (Rule 5.2(a))