Patent GB-1071421-A (GB)
Title: 2-substituted benzimidazoles as anti-fungals
IPC: A23K1/16
Filed: 1964-05-25
Abstract: 2 - (41 - Thiazolyl) - 5(6) - phenyl benzimidazole is prepared by reacting 4-thiazole carboxylic acid chloride with 3-nitro-4-aminobiphenyl, reducing the resultant N-(2-nitro-4-biphenyl) - 41 - thiazole carboxamide and acidifying with HCl to give 2-(41-thiazolyl)-5(6)-phenyl benzimidazole hydrochloride, and then converting this to the free base.  1 - Benzoyl - 2 - (41 - thiazolyl) - 5 - phenyl benzimidazole is prepared by the action of benzoyl chloride on the sodium salt of the above-mentioned 2 - (41 - thiazolyl) - 5(6)-phenylbenzimidazole.  2 - (41 - Thiazolyl) - 5(6) - (41 - methoxyphenyl) benzimidazole hydrochloride is prepared by reacting thiazole-4-carboxylic acid, thionyl chloride and p-(41-methoxyphenyl)-o-nitroaniline, reducing the resultant p-(41-methoxyphenyl) - o - nitro - N - thiazole - carbonylanilide in presence of HCl and refluxing the p-(41-methoxyphenyl) - N1 - (4 - thiazolecarbonyl)-o-phenylenediamine hydrochloride in acidified aqueous ethanol solution.  2 - (41 - Thiazolyl) - 5(6) - (21 - fluorophenyl) benzimidazole hydrochloride is prepared by reacting 2 - fluoro - 41 - aminobiphenyl with 4-cyanothiazole, refluxing a methanol solution of the hydrochloride of the resultant N-2-fluorobiphenyl(thiazole) - 4 - amidine with aqueous sodium hypochlorite and sodium hydroxide and then acidifying with HCl.  2 - (41 - Thiazolyl) - 5(6) - (41 - imidazolyl) -benzimidazole is prepared by reacting thiazole-4-carboxylic acid chloride with 4-(41-aminophenyl)imidazole, nitrating the product, reducing the nitrated material and then refluxing an acidified aqueous ethanol solution of the reduced material.  2 - (41 - Thiazolyl) - 5(6) - (21 - thiazolyl) -benzimidazole is prepared by reacting 4-cyanothiazole with 2-(41-aminophenyl)thiazole, treating the resultant N-4-(21-thiazolyl) phenyl (thiazole-4-amidine with sodium hypochlorite and refluxing an aqueous soda solution of the product.  2 - (41 - Thiazolyl) - 5(6) - dimethylamino benzimidazole is prepared by reacting thiazole-4-carboxylic acid chloride with N,N-dimethyl-p-phenylenediamine, nitrating the hydrochloride of the resultant N-(p-dimethylamino)-41-thiazole -carboxanilide and reducing the nitrated material.  2 - (41 - Thiazolyl) - 5(6) - fluorobenzimidazole is prepared by reacting 4-cyanothiazole with p-fluoroaniline and treating the resultant N - 41 - fluoro - phenyl(thiazole - 4 - amidine) with sodium hypochlorite.  By using 3,4-difluoroaniline instead of p-fluoro - aniline, 2 - (41-thiazolyl)-5,6 -difluorobenzimidazole is obtained.  p - (41 - Methoxyphenyl) - o - nitroaniline is prepared by nitrating p-phenylphenolbenzoate, hydrolysing the resultant 41-nitro-p-phenylphenol benzoate to p-hydroxy-41-nitrobiphenol, methylating this, reducing the p-methoxy-41-nitrobiphenyl to p-(41-methoxyphenyl)acetanilide, nitrating this and refluxing an acidified ethanol solution of the nitrated material.  1 - (41 - aminophenyl)imidazole is prepared by reaction of imidazole, sodium hydride and p-chloro-nitrobenzene and reduction of the N-(41-nitrophenyl)imidazole thus formed.ALSO:Foodstuffs, or containers or wrappings therefor, contain, as antifungal agents, at least one 2-R-benzimidazole in which R is a substituted or unsubstituted heterocyclic radical containing nitrogen and sulphur as the hetero atoms.  The fungicides are useful where fungi are known to attack doughs, cakes, breads, pastries, meats, cheeses, fruits (e.g. citrus fruits), vegetables, cereal, jams and jellies, brines, fish, poultry, fats and oils, juices, honey, syrup, condiments, alcoholic beverages, confections and other food products.  In particular, a composition for use in preparing bread may comprise flour, yeast and a 2-R-benzimidazole.  In examples, (a) 2-(41-thiozolyl) benzimidazole is added to bread dough, cream cheese or dog food meat, (b) 2-(21-thiazolyl) benzimidazole is added to bread dough, (c) oranges are dipped in an ethanolic solution of 2-(41-thiazolyl) benzimidazole and (d) processed cheese is wrapped in "Cellophane" (Trade Mark) coated with 2-(41-thiazolyl) benimidazole.ALSO:2-R-benzimidazoles in which R is a substituted or unsubstituted heterocyclic radical containing nitrogen and sulphur as the hetero atoms, or acid-addition salts or metal complexes thereof, are employed as fungicides.  They may be used in the treatment of plants, soil, other plant-growth media (e.g. humus, sand, manure, compost and artificially created plant growth solutions), fruits, seeds, fur and wood to prevent or reduce deterioration by applying them to a region where fungal growth is taking place or is expected.  They may be employed in solid or liquid form, e.g. as finely divided powders, granular materials, solutions, emulsions, suspensions, concentrates, emulsifiable concentrates or slurries.  They may be employed in compositions which also include finely divided dry or liquid diluents, extenders, fillers, conditioners, excipients (including various clays, diatomaceous earth and talc), water, and organic liquids (e.g. C1-C5 alkanols, kerosene, benzene, toluene, other petroleum distillate fractions or mixtures thereof).  Thus, an antifungal composition for application to plants comprises a 2-R-benzimidazole, where R is as defined above, or an acid-addition salt or metal complex thereof, together with a liquid diluent containing a wetting or emulsifying agent and/or a solid diluent.  In examples, (a) soil is treated with 2-(4\sv-thiazolyl) benzimidazole, (b) bean, celery or tomato plants are sprayed with an aqueous solution of 2-(4\sv-thiazolyl) benzimidazole and (c) oranges are treated with a composition consisting of 2-(4\sv-thiazolyl) benzimidazole and ethanol or wax. Tests are described in which compositions containing the following benzimidazoles (in aqueous solution, in some cases with added dimethyl formamide or the material known under the trade mark "Ewulphor") are evaluated as fungicides:-2-[3\sv-(1\sv,2\sv,5\sv-thiadiazolyl)] benzimidazole, 2-(2\sv-thiazolyl) benzimidazole, 2-(4\sv-thiazolyl)-5-methoxy benzimidazole, 1-acetyl-2-(4\sv-thiazolyl) benzimidazole, 2-(4\sv-thiazolyl)-5,6-difluoro benzimidazole, 2-(4\sv-thiazolyl)-5-fluoro benzimidazole, 2-(4\sv-thiazolyl)-5-(4\sv-fluorophenyl) benzimidazole hydrochloride 2-(4\sv-thiazolyl)-5-bromo benzimidazole, 2-(4\sv-thiazolyl)-5-phenyl benzimidazole, 2-(4\sv-thiazolyl)-5-(2\sv-fluoro-phenyl) benzimidazole hydrochloride, 1-benzoyl-2-(4\sv-thiazolyl) benzimidazole, 2-(4\sv-thiazolyl)-5-(4\sv-methoxy-phenyl) benzimidazole hydrochloride, 2-(4\sv-thiazolyl)-5-phenoxy-6-phenyl benzimidazole hydrochloride, 2-(2\sv-methyl-4\sv-thiazolyl) benzimidazole, 2-[4\sv-(1\sv,2\sv,3\sv-thiadiazolyl)] benzimidazole, 2-(4\sv-thiazolyl) benzimidazole hydrochloride and the Cu, Pb, Zn, Fe, Ce, Hg, Mn and Ni complexes of 2-(4\sv-thiazolyl) benzimidazole. A pharmaceutical or veterinary formulation for use in combating fungal infections comprises a 2-R-benzimidazole, wherein R is as defined above, together with an inert externally-applicable pharmaceutical or veterinary carrier or diluent.  The formulation may be in the form of a lotion, cream, jelly, ointment or dusting powder. Two or more of the benzimida zoles may be used together or the benzimidazole(s) may be used with other fungicidally action materials.  For instance, 2-(4\sv-thiazolyl) benzimidazole may be mixed with sorbic acid or its salts, propionic acid or its salts, mycostatin, sodium diacetate, trichomycin, amphotercin, griseofulvin, undecylenic acid, chlorquin adol, 5,7-dichloro-8-hydroxyquinoline, sodium o-phenylphenate, o-phenylphenol, biphenyl, chlorinated phenols, sodium benzoate, dehydroaceticacid or its salts, or esters of p-hydroxybenzoic acid.  Mixtures of the benzimidazole with antibacterial materials may also be employed especially in cosmetics and germicidal soaps.  In examples, (i) a water-insoluble ointment comprises 2-(2\sv-methyl-4\sv-thiazolyl) benzimidazole, (ii) a water-soluble ointment comprises 2-[3\sv-(1\sv,2\sv,5\sv-thiadiazolyl)] benzimidazole and (iii) a cream preparation for topical application comprises 2-(4\sv-thiazolyl) benzimidazole.  The compositions suitable for administration to domestic animals of Specification 948,635, and the pharmaceutical or veterinary compositions of Specification 987,069, are disclaimed.