Patent DE-102008048709-A1 (DE)
Title: Tempering tissue parts, comprises introducing containers of e.g. plastic into tempering device, which allows tempering over cryopreservation and storing up to depreservation and carrying out defrosting by pulsed, superheated steam strokes
Inventor: QUINGER JOACHIM
IPC: A01N1/02
Filed: 2008-09-24
Abstract: Tempering tissue parts by cryopreservation with a subsequent heating regime after introducing tissue parts in a container (1), adding cryomedium into the container and subjecting the regime to a predetermined temperature, comprises introducing one or more containers of plastic or metal into a tempering device, which allows a continuous tempering over the cryopreservation and storing up to the depreservation, and carrying out the defrosting by pulsed, superheated steam strokes, which are conducted by the interior of the temperature transformer for the perfusion. Tempering tissue parts by cryopreservation with a subsequent heating regime after introducing tissue parts in a container (1), adding cryomedium into the container and subjecting the regime to a predetermined temperature, comprises introducing one or more containers of plastic or metal into a tempering device, which allows a continuous tempering over the cryopreservation and storing up to the depreservation, where: the cryopreservation is executed in such a way that after the addition of a cryomedium; the containers are locked between heat contact surfaces (7) having a very high pressure but at least with a pressure of 1 kg/cm 2>; and the temperature range of 4[deg] C to at least -40[deg] C is passed through very quickly by the supply of gaseous cooling medium into the interior of temperature transformer, and carrying out the defrosting by pulsed, superheated steam strokes, which are conducted by the interior of the temperature transformer for the perfusion, where the heat contact surfaces between the containers containing the biological material, and the device are arranged when reheating the required melting energy are so quickly fed that a temperature rate is increased to greater than 30 K/minute between a storage temperature of below -28[deg] C and the emerging temperature of above 20[deg] C. An independent claim is included for a device comprising a container (1), which consists of biocompatible and medically safe plastics or metals provided with sealing elements (3), which consist of cold-resistant biocompatible- and elastic material with a spring effect, where the container is fitted with its heat transfer surfaces (2), in a hollow body lying towards each other with heat contact surfaces (7), where the hollow body consists of highly conductive metals and equipped with a cavity (5), which continuously or discontinuously incorporates and delivers heat transfer by connections (6), where the hollow bodies are fitted between rigid plates and an elastic, insulating material is provided between hollow- and rigid plates, which hold a contact pressure and compensate the measurement difference of the container, and the rigid plates are pressed together with a defined pressure, where the contact pressure is generated by suitable mechanical devices such as springs, eccentric tappet or threaded connections.