Patent SG-11201907012V-A (SG)
Title: Molecular switches
Inventor: HELD MARK ANTON
IPC: C12N15/53
Filed: 2018-01-30
Abstract: 90 80 - 70 - 60 - 50 40 30 20 7 10 - 0 C.) (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 02 August 2018 (02.08.2018) WIPO I PCT mu °million °nolo 01IIII111111mOIIIIII (10) International Publication Number WO 2018/140936 Al (51) International Patent Classification: C12N 9/04 (2006.01) C12N 15/53 (2006.01) C12N 9/88 (2006.01) C12N 15/60 (2006.01) C12N 15/52 (2006.01) (21) International Application Number: PCT/US2018/015925 (22) International Filing Date: 30 January 2018 (30.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/451,819 30 January 2017 (30.01.2017) US 62/504,626 11 May 2017 (11.05.2017) US 62/512,312 30 May 2017 (30.05.2017) US 62/588,985 21 November 2017 (21.11.2017) US (71) Applicant: INTREXON CORPORATION [US/US]; 1750 Kraft Drive, Suite 1400, Blacksburg, Virginia 24060 (US). (72) Inventors: HELD, Mark Anton; 1030 45th Street, REAR, Emeryville, California 94608 (US). ZHAO, Xinhua; 2771 Alliston Loop, Dublin, California 94568 (US). CHAO, Lily Yuin; 72 Langton Street, Unit 3, San Francisco, Califor- nia 94103 (US). TRINH, Na; 1839 Ygnacio Valley Road #181, Walnut Creek, California 94598 (US). KEALEY, James; 945 Midpine Way, Sebastopol, California 95472 (US). DIETZEL, Kevin Lee; 963 Fassler Avenue, Pacifi- ca, California 94044 (US). (74) Agent: KELLY, David A. et al.; Barnes & Thornburg LLP, 11 South Meridian Street, Indianapolis, IN 46204-3535 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, W O 20 18/ 140936 Al (54) Title: MOLECULAR SWITCHES (57) : Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as 2,3-BDO; 1,4-BDO; isobutyralde- hyde; isobutanol; 1-butanol; n-butanol; ethanol; fatty alcohols; and fatty acid methyl ester are disclosed. For example, genetically modified methanotrophs that are capa- ble of generating 2,3-BDO; 1,4-BDO; isobutyraldehyde; isobutanol; 1-butanol; n-bu- tanol; ethanol; fatty alcohols; and fatty acid methyl ester at high titers from a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also disclosed. These microorganisms and methods make use of molecular switches to regulate gene expression. [Continued on next page] WO 2018/140936 Al MIDEDIMOMMIDIREEMOMMEENCIMOMEN EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to the identity of the inventor (Rule 4.170) as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))