Patent SG-11201907504P-A (SG)
Title: Method and cell line for production of phytocannabinoids and phytocannabinoid analogues in yeast
Inventor: MOOKERJEE SHOHAM
IPC: C12N15/81
Filed: 2018-02-19
Abstract: International Publication Date (19) World Intellectual Property Organization International Bureau (10) International Publication Number WO 2018/148848 Al omit VIII °nolo VIII mom Imo oimIE Fig. 10 HO A GPP OH OH Methyl Oliveto! OH 0 HO Malonyl CoA Methyl CBG (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) 23 August 2018 (23.08.2018) WIP0 I PCT (51) International Patent Classification: 141 Avenue du President-Kennedy, Local SB-5230, Mon- C12P 17/02 (2006.01) Cl 2N 15/54 (2006.01) treal, Quebec H2X 1Y4 (CA). WILTSHIRE, Zachary, C07C 39/08 (2006.01) Cl 2N 15/81 (2006.01) Douglas; c/o 141 Avenue du President-Kennedy, Lo- CO7C 65/05 (2006.01) Cl 2N 9/00 (2006.01) cal SB-5230, Montreal, Quebec H2X 1Y4 (CA). CHEN, C12N 1/19 (2006.01) C12N 9/10 (2006.01) Kevin, John; c/o 141 Avenue du President-Kennedy, Local C12N 15/52 (2006.01) SB-5230, Montreal, Quebec H2X 1Y4 (CA). (21) International Application Number: PCT/CA2018/050189 (22) International Filing Date: 19 February 2018 (19.02.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/460,526 17 February 2017 (17.02.2017) US (71) Applicant: HYASYNTH BIOLOGICALS INC. [CA/CA]; 141 Avenue du President-Kennedy, Local SB-5230, Montreal, Quebec H2X 1Y4 (CA). (72) Inventors: MOOKERJEE, Shoham; c/o 141 Avenue du President-Kennedy, Local SB-5230, Montreal, Quebec H2X 1Y4 (CA). CAMPBELL, Alexander, James; c/o (74) Agent: WOOD, David et al.; BORDEN LADNER GER- VAIS LLP, 100 Queen Street, Suite 1300, Ottawa, Ontario K11 3 1J9 (CA). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (54) Title: METHOD AND CELL LINE FOR PRODUCTION OF PHYTOCANNABINOIDS AND PHYTOCANNABINOID ANA- LOGUES IN YEAST GPP (57) : A method and cell line for producing phytocannabinoids and phytocannabinoid analogues in yeast. The method applies, and the cell line includes, a yeast cell transformed with a polyketide synthase CDS and a cytosolic prenyltransferase CDS. The polyke- tide synthase enzyme catalyzes synthesis of olivetol or methyl-olivetol, and may include Cannabis sativa olivetolic acid synthase or Dictyostelium discoideum polyketide synthase (\"DiPKS\"). The yeast cell may be modified to include a phosphopantethienyl transferase for increased activity of DiPKS. The yeast cell may be modified to mitigate mitochondrial acetaldehyde catabolism for increasing malonyl-CoA available for synthesizing olivetol or methyl-olivetol. The prenyltransferase enzyme catalyzes synthesis of cannabigerol or a cannabigerol analogue, and may include an ct1313ct cytosolic prenyltransferase enzyme from Streptomyces sp CL190. The yeast cell may be modified to mitigate depletion of geranyl pyrophosphate for increasing available geranyl pyrophosphate for prenylation. [Continued on next page] WO 2018/148848 Al MIDEDIMOMOIDEIREEMOMMEIEHEIFIONVOIMIE GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))