Patent EA-003750-B1 (EA)
Title: Herbal extract composition with immune-boosting capability and the method of preparing and use
IPC: A61K36/82
Filed: 1999-06-19
Abstract: 1. An herbal extract composition for stimulating cell mediated immunity, comprising extract of ARUM. 2. An herbal extract composition as recited in claim 1, further comprising extract of POMEGRANATE. 3. An herbal extract composition as recited in claim 1, further comprising extract of HIBISCUS. 4. An herbal extract composition as recited in claim 1, further comprising extract of TEA. 5. An herbal extract composition as recited in claim 2, further comprising extract of HIBISCUS. 6. An herbal extract composition as recited in claim 2, further comprising extract of TEA. 7. An herbal extract composition as recited in claim 5, further comprising extract of TEA. 8. An herbal extract composition for stimulating cell mediated immunity, comprising extract of POMEGRANATE. 9. An herbal extract composition as recited in claim 8, further comprising extract of HIBISCUS. 10. An herbal extract composition as recited in claim 8, further comprising extract of TEA. 11. An herbal extract composition as recited in claim 9, further comprising extract of TEA. 12. An herbal extract composition as recited in claim 7, characterized in that the extracts of ARUM, POMEGRANATE, HIBISCUS and TEA are aqueous extracts. 13. The herbal extract composition of claim 12, wherein said composition further comprises at least one cytokine. 14. The herbal extract composition of claim 13, wherein said cytokine is an interleukin. 15. The herbal extract composition of claim 13, wherein said cytokine is an interferon. 16. The herbal extract composition of claim 12, wherein said composition further comprises at least on tumor necrosis factor. 17. The herbal extract composition of claim 12, wherein said composition further comprises at least one immunoglobulin. 18. The herbal extract composition of claim 12, wherein said composition further comprises polysaccharide. 19. The herbal extract composition of claim 13, wherein said composition further comprises proteins. 20. The herbal extract composition of claim 13, wherein said composition further comprises RNA. 21. The herbal extract composition of claim 13, wherein said composition further comprises DNA. 22. The herbal extract composition of claim 13, wherein said composition contains approximately equal portions of said aqueous extracts. 23. A method of preparing an herbal extract composition of claim 2 for stimulating cell mediated immunity, comprising the steps of: (a) preparing extract of ARUM by boiling dried, finely crushed ARUM in water; and (b) preparing extract of POMEGRANATE by boiling dried, finely crushed POMEGRANATE in water; and (c) combining said extract of ARUM and said extract of POMEGRANATE and boiling said combined extracts of ARUM and POMEGRANATE. 24. A method of preparing an herbal extract as recited in claims 3-7, comprising the steps of: (a) preparing extract of ARUM by boiling dried, finely crushed ARUM in water; and (b) preparing extract of POMEGRANATE by boiling dried, finely crushed POMEGRANATE in water; and (c) preparing extract of HIBISCUS by boiling dried, finely crushed HIBISCUS in water; (d) preparing extract of TEA by boiling dried, finely crushed TEA in water; and (e) combining said extract of HIBISCUS and said extract of TEA with said combined extracts of ARUM and POMEGRANATE and boiling said combined extracts of ARUM, POMEGRANATE, HIBISCUS, TEA. 25. A method for stimulating in vitro cell mediated immunity comprising contacting cells with an efficacious amount of a preparation consisting essentially of extract by one of claims 1-11. 26. A method for stimulating in vitro cell mediated immunity as recited in claim 25, wherein said cells comprise cells from normal patients. 27. A method for stimulating in vitro cell mediated immunity as recited in claim 25, wherein said cells comprise cells from patients having leukemia. 28. A method for stimulating in vitro cell mediated immunity as recited in claim 25, wherein said cells comprise cells from patients having renal failure. 29. A method for stimulating in vitro lymphocyte blastogenesis, comprises contacting lymphocyte suspensions with an efficacious amount of a preparation consisting essentially of extracts by one of claims 1-11. 30. A method for stimulating in vitro cell mediated immunity as recited in claim 29, wherein said lymphocyte suspensions comprise lymphocytes from normal patients. 31. A method for stimulating in vitro cell mediated immunity as recited in claim 29, wherein said lymphocyte suspensions comprise lymphocytes from patients having leukemia. 32. A method for stimulating in vitro cell mediated immunity as recited in claim 29, wherein said lymphocyte suspensions comprise lymphocytes from patients having renal failure. 33. A method for stimulating in vitro cytokine production, comprising contacting peripheral mononuclear blood cells with an efficacious amount of a preparation consisting essentially of extracts by one of claims 1-11. 34. A method for stimulating in vitro cell mediated immunity as recited in claim 33, wherein said peripheral mononuclear blood cells are obtained from normal patients. 35. A method for stimulating in vitro cell mediated immunity as recited in claim 33, wherein said peripheral mononuclear blood cells are obtained from patients having leukemia. 36. A method for stimulating in vitro cell mediated immunity as recited in claim 33, wherein said peripheral mononuclear blood cells are obtained from patients having renal failure. 37. A method for inhibiting in vitro HIV infection, comprising contacting cells with an efficacious amount of a preparation consisting essentially of extract by one of claims 1-11. 38. A method for inhibiting in vitro binding of gp120 to MT-4 cells, comprising contacting said MT-4 cells with an efficacious amount of a preparation consisting essentially of extract by one of claims 1-11.