Patent SG-11201910070P-A (SG)
Title: Universal short adapters for indexing of polynucleotide samples
Inventor: SINGER TATJANA
IPC: C12Q1/6869
Filed: 2018-05-07
Abstract: 17 index primer 222 220 218 204 15 index primer 202 214 Short universal adapter (Y-shaped) 223 216 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 15 November 2018 (15.11.2018) WIPO I PCT omit VIII °nolo Holommoillionomom oimIE (10) International Publication Number WO 2018/208699 Al (51) International Patent Classification: C12Q 1/6869 (2018.01) Cl 2N 15/10 (2006.01) GOOF 19/22 (2011.01) (21) International Application Number: PCT/US2018/031459 (22) International Filing Date: 07 May 2018 (07.05.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/503,272 08 May 2017 (08.05.2017) US 62/524,390 23 June 2017 (23.06.2017) US (71) Applicant: ILLUMINA, INC. [US/US]; 5200 Illumina Way, San Diego, California 92122 (US). (72) Inventors: SINGER, Tatjana; 5200 Illumina Way, San Diego, California 92122 (US). KELLEY, Ryan; 5200 Il- lumina Way, San Diego, California 92122 (US). BEAN, Gordon; 5200 Illumina Way, San Diego, California 92122 (US). VERMAAS, Eric; 5200 Illumina Way, San Diego, California 92122 (US). (74) Agent: ZHOU, Feng et al.; WEAVER AUSTIN VIL- LENEUVE & SAMPSON LLP, P. 0. BOX 70250, OAK- LAND, California 94612-0250 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). = (54) Title: UNIVERSAL SHORT ADAPTERS FOR INDEXING OF POLYNUCLEOTIDE SAMPLES Figure 2C (57) : The disclosed embodiments concern index oligonucleotides configured to identify sources of samples of nucleic acids and methods, apparatus, systems and computer program products for identifying and making the index oligonucleotides. In some implementations, the index oligonucleotides include a set of index sequences, a Hamming distance between any two index sequences of the set of index sequences meeting one or more criteria. System, apparatus, and computer program products are also provided for determining a sequence of interest using the index oligonucleotides. [Continued on next page] W O 20 18/ 208699 Al WO 2018/208699 Al MIDEDIMOMOIDEIRDERIHNOIREIHRIONVOIMIE Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))