Patent SG-11201806647V-A (SG)
Title: Systems and methods for allergen detection
Inventor: GILBOA-GEFFEN ADI
IPC: G01N33/50
Filed: 2017-03-10
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/160616 Al 21 September 2017 (21.09.2017) WIPO I PCT 1111111111111101110101011111010111110111011110011011111101111111111111111110111111 (51) International Patent Classification: B01J 19/00 (2006.01) G01N 33/02 (2006.01) C12M 1/34 (2006.01) G01N 33/50 (2006.01) C12Q 1/68 (2006.01) (21) International Application Number: PCT/US2017/021737 (22) International Filing Date: 10 March 2017 (10.03.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/308,376 15 March 2016 (15.03.2016) US 62/308,377 15 March 2016 (15.03.2016) US (71) Applicant: DOTS TECHNOLOGY CORP. [US/US]; 16 Tech Circle, 2nd Floor, Natick, Massachusetts 01760 (US). (72) Inventors: GILBOA-GEFFEN, Adi; 31 Alden Rd., Way- land, Massachusetts 01778 (US). BABU BROWN, Re- nuka; 536 Glen Road, Weston, Massachusetts 02493 (US). STIDHAM, Sarah; 12 James St. #3, Brookline, Mas- sachusetts 02446 (US). VILLAREAL, Valerie; 129 Com- monwealth Avenue, Boston, Massachusetts 02116 (US). YOUNG, Adam J.; 82 Monroe Street, Dedham, Mas- sachusetts 02026 (US). ANTHONY, Joshua Glenn; 39 Sunset Rock Lane, Reading, Massachusetts 01867 (US). MURPHY, Patrick; 16 Valentine St., Cambridge, Mas- sachusetts 02139 (US). KEPLER, John H.; 19 Summer Street, Lexington, Massachusetts 02420 (US). JACOBS, Adam; 15 Crestwood Drive, Hollis, New Hampshire 03049 (US). (74) Agents: WARD, Donna T. et al.; DT Ward, P.C., 142A Main Street, Groton, Massachusetts 01450 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, [Continued on next page] (54) Title: SYSTEMS AND METHODS FOR ALLERGEN DETECTION Fig. 1a 1 W O 20 17 / 1606 16 Al (57) : The present invention provides systems, devices, signaling poly- nucleotides (SPNs), detection agents and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The de- tection system includes a separate sampler, at least one disposable detection vessel for receiving and processing a test sample and a detection device for measuring a fluores- cent signal. SPNs derived from aptamer that bind allergens are also provided as al- 30 lergen detection agents. SPNs have a single open structure, and are probed with a fluorophore. Changes in fluorescence polarization of detection agents upon the binding of allergens are measured to cal- culate the allergen content in a sample. WO 2017/160616 Al MIDEDIMOMOIDEIRMEMOMOHINIMEMOHIMMOIMIE LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))