Patent EA-001741-B1 (EA)
Title: Dermatological and cosmetic compositions for topical application comprising sphingomyelinase to increase the levels of skin and mucosal ceramides
Inventor: CAVALIERE RENATA MARIA ANNA
IPC: C12N1/21
Filed: 1997-11-14
Abstract: 1. Use of sphingomyelinase for producing dermatological and/or cosmetic compositions suitable to be topically applied, to increase the levels of skin and mucosal ceramides, characterised in that the sphingomyelinase of Gram-positive bacteria, Gram-negative bacteria, lactic bacteria or mixtures thereof is used. 2. The use of Claim 1 for producing dermatological compositions suitable to be topically applied for the prevention or therapeutical treatment of atopic eczema, dermatosis and dermatitis, in particular atopic dermatitis, psoriasis, ichthyosis, Fabry's disease, Gaucher's disease, Tay-Sachs disease and Sjogren-Larsson's syndrome. 3. The use of Claims 1 or 2, characterized in that the lactic bacteria are selected from the group comprising Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus casei, Lactobacillus catenaforme, Lactobacillus cellobiosus, Lactobacillus crispatus, Lactobacillus curvatus; Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus jensenii, Lactobacillus leichmanii, Lactobacillus minutus, Lactobacillus plantarum, Lactobacillus rogosae, Lactobacillus salivarius, Bifidobacterium adolescentis, Bifidobacterium angulatum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium catenulatum, Bifidobacterium dentium, Bifidobacterium eriksonii, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium plantarum, Bifidobacterium pseudocatenulatum, Bifidobacterium pseudolongum, Steptococcus lactis, Streptococcus raffinolactis and Streptococcus thermophilus. 4. The use according to any of Claims 1-3 for preparing pharmaceutical or cosmetic compositions suitable for transdermal administration. 5. The use according to any of Claims 1-4, characterized in that sphingomyelinase before use is evolved from bacterial cells or it is used in the form of lyophilized or sonicated bacterial cells. 6. A dermatological or cosmetic composition comprising an amount of sphingomyelinase effective for bringing about an increase in the level of skin and mucosal ceramides, characterised in that the sphingomyelinase is obtained from Gram-positive bacteria, Gram-negative bacteria, lactic bacteria or mixtures thereof. 7. The composition of Claim 6, characterized in that the lactic bacteria are selected from the group comprising Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus buchneri, Lactobacillus casei, Lactobacillus catenaforme, Lactobacillus cellobiosus, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus jensenii, Lactobacillus leichmanii, Lactobacillus minutes, Lactobacillus plantarum, Lactobacillus rogosae, Lactobacillus salivarius, Bifidobacterium adolescentis, Bifidobacterium angulatum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium catenulatum, Bifidobacterium dentium, Bifidobacterium eriksonii, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium plantarum, Bifidobacterium pseudocatenulatum, Bifidobacterium pseudolongum, Steptococcus lactis, Streptococcus raffinolactis and Streptococcus thermophilus. 8. The composition of Claims 6 or 7, characterized in that sphingomyelinase before use is evolved from bacterial cells or it is used in the form of lyophilized or sonicated cells. 9. The composition of any one of Claims 6-8, characterized in that it comprises from 1x10<2> to 1x10<15> lactic bacteria/gram of composition. 10. The composition of any one of Claims 6-9, characterized in that it further comprises exogenous ceramide or products containing exogenous ceramide, sphingomyelines, fatty acids, cholesterol, ceramidase inhibitors, protease inhibitors, immunomodulators, vitamins, growth factors, surfactants, emulsifiers, stabilisers, lipids, rheological additives, humidifiers, antioxidants, preservatives, colouring agents, lakes, pigments, auxiliary substances (e.g. acids, bases, propellants) and functional substances (astringents, antiseborrheic agents, anti-dandruff agents, deodorants, skin purifiers, keratogenous agents, moisturisers, anti-xerosis agents, smoothing agents, screens, sunscreens, pigmenting agents, anti-depigmenting agents, emollients, restores, eutrophic agents, anti-wrinkle agents, antiradicals, stiffeners, anti-stretch-mark agents, vasoprotectors, anti-skin-rash agents, soothing agents, anti-cellulitis agents, tonifying agents, stimulants, hypereluting agents, epilators, nail protectors). 11. The composition of any one of Claims 6-10 in the form of creams, ointments, lotions, capsules, pearls, ovules, mascara, eyewashes, toothpaste, mouthwashes, lipsticks, liposomes, soap, shaving soap, tonics, douches, enteroclysis solutions, shampoos, anti-dandruff preparations, impregnated and/or medicated bandage or gauze, patches medicated emulsions, transdermal gels or patches.