Patent SG-11201811370X-A (SG)
Title: A rationally developed african swine fever attenuated virus strain protects against challenge with parental virus georgia 2007 isolate
Inventor: BORCA MANUEL V
IPC: A61K39/12
Filed: 2017-06-26
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property C.--.` MD 11011110111010101111101 11010101110111111011011101111011110111 OEN Organization International Bureau (10) International Publication Number 03 (43) International Publication Date ......•' WO 2018/005358 Al 04 January 2018 (04.01.2018) W I PO I PCT (51) International Patent Classification: THE UNIVERSITY OF CONNECTICUT [US/US]; 400 C07K 14/005 (2006.01) A61K 39/12 (2006.01) Farmington Avenue, MC 6400, Farmington, Connecticut (21) International Application Number: 06032 (US). PCT/US2017/039277 (72) Inventors: BORCA, Manuel V.; 40550 Route 25, Ori- (22) International Filing Date: ent Point, New York 11957 (US). GLADUE, Douglas 26 June 2017 (26.06.2017) P.; 40550 Route 25, Orient Point, New York 06437 (US). HOLINKA-PATTERSON, Lauren G.; 40550 Route 25, (25) Filing Language: English Orient Point, New York 11944 (US). RISATTI, Guillermo R.; 61 N. Eagleville Road, Storrs, Connecticut 06498 (US). (26) Publication Language: English O'DONNELL, Vivian K.; 61 N. Eagleville Road, Storrs, (30) Priority Data: Connecticut 06269 (US). 15/200,407 01 July 2016 (01.07.2016) US (74) Agent: GOLDBERG, Joshua B.; Nath, Goldberg & Mey- (71) Applicants: THE UNITED STATES OF AMERI- er, 112 S. West Street, Alexandria, Virginia 22314 (US). CA, AS REPRESENTED BY THE SECRETARY (81) Designated States (unless otherwise indicated, for every OF AGRICULTURE [US/US]; 1400 Independence Ave. kind of national protection available): AE, AG, AL, AM, S.W., Washington, District of Columbia 20250 (US). AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (54) Title: A RATIONALLY DEVELOPED AFRICAN SWINE FEVER ATTENUATED VIRUS STRAIN PROTECTS AGAINST CHALLENGE WITH PARENTAL VIRUS GEORGIA 2007 ISOLATE Fig. 1A : 40 50 .218W-0 RELKLTAIFFS OAPSIIKEK26 AIOWILME OLPCTRCTIO AFSTI1PKNPT, Malay/ Li1-20/1 , 7 T G Pma 18 A , R Pr5 18 Killaan3 19 I , Crl Cr3 Tengam1I82) Fair...W/95A 21 Wildabeaalaagte 18 Kiaak.,1(84) 22 Vintarla Falls Lairs (67) 2 rIganda(61) 2 L70 K./5 2 Haiti apan0ar(51) La Cranja(63) Liabon(50) Lea(35) . •I•.. I 00 90 100 110 120 ASFV-0 2 TLNNSE WrFAFENNV14 KILLNKKUSW 8152KNITE08 ILKTIKvOiT DvicAw8KL. malawi Li1-20/1 17 K N Fra 18 . Pr5 18 Killean3 K 19 Cri in Cr3 Tangani(52) Fairfield/96/1 21 Wild.haaalaagta 18 almakma(64) 22 Viat.ria Palls Moira (67) 178anda(51) E70 E75 Haiti Spencer 151) , Im Cranja(03/ 2 11 Liahon(60) Lae (35) GC (57) : African swine fever virus (ASFV) is the etiological agent of a contagious, often lethal viral disease of domestic pigs. imr) The control of African Swine Fever (ASF) has been hampered by the unavailability of vaccines. Experimental vaccines have been en k i n derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated ASFVs; however, these vaccines are only C:::: ) successful when protecting against homologous viruses. We have constructed a recombinant A9GL/AUK virus derived from the highly 0 --.... virulent ASFV Georgia 2007 (ASFV-G) isolate by deleting the specific virulence-associated 9GL (B119L) and the UK (DP96R) genes. GC ,- 1 In vivo, ASFV-G A9GL/AUK administered intramuscularly to swine even at relatively high doses (10 6 HAD50) does not induce disease. 0 N C [Continued on next page] WO 2018/005358 Al MIDEDIMOMOIDEIREEMOOVIREINIMMEHOM CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a)) Importantly, animals infected with 10 4 or 10 6 HAD 5 0 are solidly protected against the presentation of clinical disease when challenged at 28 days post infection with the virulent parental strain Georgia 2007.