Patent SG-11201900442P-A (SG)
Title: Sorting of t lymphocytes in a microfluidic device
Inventor: LOUTHERBACK KEVIN D
IPC: G01N1/34
Filed: 2017-07-21
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#111011110111010101111101 1101001111113111011111010111n#011# Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\" WO 2018/018017 Al 25 January 2018 (25.01.2018) WI P0 I P C T (51) International Patent Classification: (72) Inventors; and A61K 35/14 (2015.01) GO1N 1/34 (2006.01) (71) Applicants: LOUTHERBACK, Kevin D. [US/US]; 5858 A61K 35/28 (2015.01) Horton Street, Suite 320, Emeryville, California 94608 (US). BRONEVETSKY, Yelena [US/US]; 5858 Hor- (21) International Application Number: PCT/US2017/043395 ton Street, Suite 320, Emeryville, California 94608 (US). BEEMILLER, Peter J. [US/US]; 5858 Horton Street, (22) International Filing Date: Suite 320, Emeryville, California 94608 (US). WANG, Xi- 21 July 2017 (21.07.2017) aohua [CN/US]; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). CHAPMAN, Kevin T. [US/US]; (25) Filing Language: English 5858 Horton Street, Suite 320, Emeryville, California 94608 (26) Publication Language: English (US). (30) Priority Data: (74) Agent: HORTON, Kenneth E.; Kirton McConkie, 36 S. 62/365,372 21 July 2016 (21.07.2016) US State Street, Suite 1900, Salt Lake City, Utah 84111 (US). (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 (81) Designated States (unless otherwise indicated, for every Horton Street, Suite 320, Emeryville, California 94608 kind of national protection available): AE, AG, AL, AM, (US). AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, = (54) Title: SORTING OF T LYMPHOCYTES IN A MICROFLUIDIC DEVICE = 715 ---% 1 = = — = EE = = _ = = = = = - = 1 i 708/710 720 • '' ' . ,, - - - _ 730 ,-, FIG. 7B 702 IN Il (57) : Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid 0 of; sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be Il configured to have a critical size (D o ) that separates activated T lymphocytes from nave T lymphocytes. Also provided are microfluidic --... © devices having an array of posts configured to separate activated T lymphocytes from nave T lymphocytes, compositions enriched for Ot T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating 1-1 c:::: ) subjects suffering from a pathogenic disorder or cancer by administering such compositions. ei C [Continued on next page] WO 2018/018017 Al MIDEDIMOMOIDEIREEMOOMMOHMOIRMERMIS KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))