Patent SG-11201909712T-A (SG)
Title: Ketoreductase polypeptides and polynucleotides
Inventor: MOORE JEFFREY
IPC: C12N9/00
Filed: 2018-04-13
Abstract: selective oxidation 2. selective reduction NAD S-selective NADH KRED-NADH-102 R-selective KRED-101 NADP NADPH NADH Oxidase H2O - 02 (1a) .0 , 110H (1) (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 November 2018 (01.11.2018) WIPO I PCT o iimion ono o oloim imio (10) International Publication Number WO 2018/200214 A2 (51) International Patent Classification: C12P 7/04 (2006.01) C12N 9/00 (2006.01) (21) International Application Number: PCT/US2018/027450 (22) International Filing Date: 13 April 2018 (13.04.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/491,161 27 April 2017 (27.04.2017) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, CA 94063 (US). (72) Inventors: MOORE, Jeffrey, C.; 126 East Lincoln Av- enue, Rahway, NJ 07065 (US). LIANG, Jack; 200 Penob- scot Drive, Redwood City, CA 94063 (US). PENFIELD, Jonathan; 11269 Skyline Ct., Truckee, CA 96161 (US). NAZOR, Jovana; 200 Penobscot Drive, Redwood City, CA 94063 (US). DELLAS, Nikki; 200 Penobscot Drive, Redwood City, CA 94063 (US). MITCHELL, Vesna; 200 Penobscot Drive, Redwood City, CA 94063 (US). DUAN, Da; 200 Penobscot Drive, Redwood City, CA 94063 (US). FARASAT, Iman; 126 East Lincoln Avenue, Rahway, NJ 07065 (US). RODRIGUEZ-GRANILLO, Agustina; 126 East Lincoln Avenue, Rahway, NJ 07065 (US). MURPHY, Grant; 126 East Lincoln Avenue, Rahway, NJ 07065 (US). MARSHAL, Nicholas; 126 East Lincoln Avenue, Rahway, NJ 07065 (US). (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, CA 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, = (54) Title: KETOREDUCTASE POLYPEPTIDES AND POLYNUCLEOTIDES OH Na2PO4 Na2P03 phosphite dehydrogenase PDH-101 FIG. 1 W O 20 18/ 200214 A2 (57) : The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved prop- erties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketore- ductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase en- zymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system. [Continued on next page] WO 2018/200214 A2 IMIOHOIMIREIHHIMINIRIlinThin KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: without international search report and to be republished upon receipt of that report (Rule 48.2(g)) with sequence listing part of description (Rule 5.2(a))