Patent SG-11201806002S-A (SG)
Title: Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process
Inventor: GENOVESE NICHOLAS
IPC: C12N15/85
Filed: 2017-01-17
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization MD 1111101110101011111 HO 11111 01110111010111110101110110111110111011# International Bureau .. .... ..Yejd ..... .....!;,,, (10) International Publication Number (43) International Publication Date WO 2017/124100 Al 20 July 2017 (20.07.2017) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A23L 1/31 (2006.01) C12N 15/85 (2006.01) kind of national protection available): AE, AG, AL, AM, C12N 5/077 (2010.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/US2017/013782 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (22) International Filing Date: KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, 17 January 2017 (17.01.2017) MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (25) Filing Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (26) Publication Language: English TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 62/278,869 14 January 2016 (14.01.2016) US (84) Designated States (unless otherwise indicated, for every 62/361,867 13 July 2016 (13.07.2016) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicant: MEMPHIS MEATS, INC. [US/US]; 1933 TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Davis Street, Suite 312, San Leandro, California 94577 TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (US). DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (72) Inventors: GENOVESE, Nicholas; 1517 Hayden Street, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Hayward, California 94545 (US). DESMET, Danielle SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, _ Nicole; 1921 Delaware St. #4, Berkeley, California 94709 GW, KM, ML, MR, NE, SN, TD, TG). — (US). SCHULZE, Eric; 821 Leavenworth St., Unit 53, Published: _ = San Francisco, California 94109 (US). — with international search report (Art. 21(3)) (74) Agent: MADHURI, Roy; Cooley LLP, 1299 Pennsylvania with sequence listing of description (Rule 5.2(a)) part Avenue, NW, Suite 700, Washington, DC 20004 (US). = = = = = = = = = = Il © (54) Title: METHODS FOR EXTENDING THE REPLICATIVE CAPACITY OF SOMATIC CELLS DURING AN EX VIVO 0 CULTIVATION PROCESS Il 'V (57) : A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amend - \" ments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applica- tions and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first IN exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and O p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental popula - e , ' tions. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase 0 protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application ,..1: is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus; another is from the livestock species Bos taurus.