Patent EA-003632-B1 (EA)
Title: Phytase from a monascus anka microorganism, process for producing thereof and animal feed containing said phytases
Inventor: NAGASHIMA TADASHI
IPC: A23K1/165
Filed: 1997-09-25
Abstract: 1. Phytase derived from a Monascus anka microorganism having the following properties: a) Michaelis constants (referred to hereinafter as Km) of 10 to 110 muM when phytic acid is used as a substrate; b) substrate specificity: acting on phytic acid, p-nitrophenylphosphate, D-glucose-6-phosphate, fructose-6-phosphate, D-myo-inositol-2-phosphate, D-myo-inositol-1-phosphate, D-myo-inositol-1,4-diphosphate, and adenosine triphosphate as the substrate; c) optimum pH: pH 2.5 to 6.0; d) pH stability: stable in the range of pH 2.0 to 8.0; e) optimum temperature: 45 to 50 degree C; f) temperature stability: stable up to 35 to 60 degree C; g) molecular weight: about 80 to 140 kDa (gel filtration method); and h) isoelectric point: pI 4.8 to 5.7 (chromatofocusing method). 2. Phytase derived from a Monascus anka microorganism having the following physiochemical properties: a) Km: 27 muM when phytic acid is used as a substrate; b) substrate specificity: acting on phytic acid, p-nitrophenylphosphate, D-glucose-6-phosphate, fructose-6-phosphate, D-myo-inositol-2-phosphate, D-myo-inositol-1-phosphate, D-myo-inositol-1,4-diphosphate, and adenosine triphosphate as the substrate; c) optimum pH: pH 5.5; d) pH stability: stable in the range of pH 5.5 to 6.5; e) optimum temperature: 50 degree C; f) temperature stability: stable up to 35 degree C; g) molecular weight: about 80 to 100 kDa (gel filtration method); and h) isoelectric point: pI 5.7 (chromatofocusing method). 3. Phytase derived from a Monascus anka microorganism having the following physiochemical properties: a) Km: 107 muM when phytic acid is used as a substrate; b) substrate specificity: acting on phytic acid, p-nitrophenylphosphate, D-glucose-6-phosphate, fructose-6-phosphate, D-myo-inositol-2-phosphate, D-myo-inositol-1-phosphate, D-myo-inositol-1,4-diphosphate, and adenosine triphosphate as the substrate; c) optimum pH: pH 25; d) pH stability: stable in the range of pH 2.0 to 8.0; e) optimum temperature: 45 degree C; f) temperature stability: stable up to 60 degree C; g) molecular weight: about 140 kDa (gel filtration method); and h) isoelectric point: pI 4.8 (chromatofocusing method). 4. Phytases derived from a Monascus anka microorganism having the following physiochemical properties: a) Km: 27 muM when phytic acid is used as a substrate; b) substrate specificity: acting on phytic acid, p-nitrophenylphosphate, D-glucose-6-phosphate, fructose-6-phosphate, D-myo-inositol-2-phosphate, D-myo-inositol-1-phosphate, D-myo-inositol-1,4-diphosphate, and adenosine triphosphate as the substrate; c) optimum pH: pH 5.5; d) pH stability: stable in the range of pH 5.5 to 6.5; e) optimum temperature: 50 degree C; f) temperature stability: stable up to 35 degree C; g) molecular weight: about 80 to 100 kDa (gel filtration method); and h) isoelectric point: pI 5.2 (chromatofocusing method); i) N-terminal amino acid sequence: shown in SEQ ID NO:1. 5. Phytases according to claim 1 wherein Monascus anka microorganism is IFO30873. 6. A process for producing phytases which comprises culturing a microorganism having the ability to form and accumulate until the phytases are formed and accumulated, and recovering the phytases from the culture. 7. The process according to claim 6, wherein Monascus anka microorganism is IFO30873. 8. Animal feed comprising phytases characterized by any one of claims 1 to 5.