Patent SG-11201809990T-A (SG)
Title: Microfluidic platform for investigating cell-based interactions
Inventor: PAVESI ANDREA
IPC: B01L3/00
Filed: 2015-11-09
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111 OH 110101011101111110011111H MHO HOED International Bureau ... ..... ..)d .... ..... (10) International Publication Number (43) International Publication Date WO 2016/076795 Al 19 May 2016 (19.05.2016) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every B81B 1/00 (2006.01) B81C 1/00 (2006.01) kind of national protection available): AE, AG, AL, AM, BOIL 3/00 (2006.01) Cl 2M 3/00 (2006.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/SG2015/050441 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, (22) International Filing Date: KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, 9 November 2015 (09.11.2015) MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (25) Filing Language: English SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, (26) Publication Language: English TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: (84) Designated States (unless otherwise indicated, for every 62/077,942 11 November 2014 (11.11.2014) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicant: AIM BIOTECH PTE. LTD. [SG/SG]; 51 TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Ayer Rajah Crescent, #02-12/14, Singapore 139948 (SG). TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (72) Inventors: PAVESI, Andrea; 206 Depot Road, #08-52, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Singapore 109697 (SG). LIM, Sei Hien; 701 Yishun Av- SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, enue 5, #11-300, Singapore 760701 (SG). LIM, Swee Khuan; 629 Woodlands Ring Road, #13-238, Singapore GW, KM, ML, MR, NE, SN, TD, TG). — 730629 (SG). KUAN, Chee Mun; 136B Hillview Avenue, Published: #03-03, Singapore 669607 (SG). — with international search report (Art. 21(3)) (74) Agent: JOYCE A. TAN & PARTNERS LLC; 8 Tem- _ = asek Boulevard, #15-04, Suntec Tower Three, Singapore 038988 (SG). = = = = (54) Title: MICROFLUIDIC PLATFORM FOR INVESTIGATING CELL-BASED INTERACTIONS = = 10 16 14 14 = 1 = _ e ON 0 CO II 0 . _ 12 ... ... .., .., ... Ili A \c) e e-e 01 14 Figure 1 b IN 11 in 16 ,tD IN 0 ---- (57) : A microfluidic platform (10) for investigating cell-based interactions in accordance with the invention, includes com - 2 prises a chip base (12) made of a suitable plastics material with the appropriate optical properties. The chip base (12) has a plurality ::::) of ports (14) in fluid communication with a microfluidic channel (16) for containing a culture medium in which cells are held. N Preferably a gas permeable laminate (20) is bonded to a bottom surface of the chip base (12). Each port 14 has an internal inlet (22), 0 connecting the port (14) with the microfluidic channel (16), and a trough (24) for containing a small reservoir of culture medium flu- id adjacent to the inlet (22) wherein, in use, culture medium can be aspirated from the microfluidic channel via the trough rather than directly via the internal inlet.