Patent SG-11201900573U-A (SG)
Title: Methods of purifying fc-containing proteins
Inventor: KAWOOYA JOHN K
IPC: C07K16/00
Filed: 2017-07-21
Abstract: 2 5 4.0 Peak 1 40 20 mnnuiMplllANla'IPJUNIIO ifif lowommotestmormem FIG. 12 er) O co O 00 O O (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 25 January 2018 (25.01.2018) W I PO I PCT Iiiimmoimiolollm °nolo ommolmollmoinifloiliollIE (10) International Publication Number WO 2018/018011 A3 (51) International Patent Classification: CO7K 1/22 (2006.01) C07K 16/00 (2006.01) CO7K 1/34 (2006.01) (21) International Application Number: PCT/US2017/043384 (22) International Filing Date: 21 July 2017 (21.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/365,943 22 July 2016 (22.07.2016) US (71) Applicant: AMGEN INC. [US/US]; One Amgen Center Drive, Thousand Oaks, California 91320-1799 (US). (72) Inventor: KAWOOYA, John K.; 12565 Spring Creek Road, Moorpark, California 93021 (US). (74) Agent: PURCELL, Angela L.; One Amgen Center Drive, Thousand Oaks, California 91320-1799 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (54) Title: METHODS OF PURIFYING FC-CONTAINING PROTEINS —Coo,,C1000.1:244.00143 2202400 Puke O. --Cure 8_03030.1.94403.14.3 2 , 30 oiteban Oup_Chrt401 dac 200 Datlini. &Pim 0... _Chnoo.lootpordex 200 22303aL elution 0.02n11 1:34P30102 20020ernl elution 0.0204 P-- Peak 3 (57) : The present invention relates to methods of purifying proteins containing Fc regions, such as antibodies and Fc fusion proteins. In particular, the present invention relates to a purification method resulting in reduced levels of aggregate protein comprising adsorbing an Fc region-containing protein to a temperature-responsive protein A resin and eluting the protein from the resin at a temperature below 35 C with an elution buffer comprising a chaotropic agent, a sugar alcohol, and at least one amino acid. Methods of separating fully assembled antibodies from half antibody forms thereof using the elution buffer are also described. [Continued on next page] WO 2018/018011 A3 MIDEDIMOMOIDEIRDERIO 01111110111111011111 011111111111101110 II TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (88) Date of publication of the international search report: 01 March 2018 (01.03.2018)