Patent SG-11201806190T-A (SG)
Title: Isotachophoresis for purification of nucleic acids
Inventor: MARSHALL LEWIS
IPC: G01N27/447
Filed: 2017-01-28
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/132630 Al 3 August 2017 (03.08.2017) WIPO I PCT 111111111111110111011111111111010111110111111110111011110110111110111111111110111111 (51) International Patent Classification: C12N 15/10 (2006.01) B81B 1/00 (2006.01) G01N 27/447 (2006.01) (21) International Application Number: PCT/US2017/015519 (22) International Filing Date: 28 January 2017 (28.01.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/288,930 29 January 2016 (29.01.2016) US (71) Applicant: PURIGEN BIOSYSTEMS, INC. [US/US]; 5700 Stoneridge Drive, Suite 100, Pleasanton, CA 94588 (US). (72) Inventors: MARSHALL, Lewis, A.; 5700 Stoneridge Drive, Suite 100, Pleasanton, CA 94588 (US). HID- DESSEN, Amy, L.; 5700 Stoneridge Drive, Suite 100, Pleasanton, CA 94588 (US). HOVERTER, Nathan, P.; 5700 Stoneridge Drive, Suite 100, Pleasanton, CA 94588 (US). ROSE, Klint, A.; 5700 Stoneridge Drive, Suite 100, Pleasanton, CA 94588 (US). SANTIAGO, Juan, G.; 440 Escondido Mall, Stanford, CA 94305-3030 (US). = (74) Agents: PETERSON, Teresa, E. et al.; Wilson Sonsini Goodrich & Rosati, 650 Page Mill Road, Palo Alto, CA 94304-1050 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) = (54) Title: ISOTACHOPHORESIS FOR PURIFICATION OF NUCLEIC ACIDS 603 609 605 606 610 607 608 601 604 1\"1 _L 1_ Zor Leaoit ;0.2 Lone 1: Sample , 612 611 Zone 3: Elution 602 FIG. 6C (57) : The present disclosure relates to fluidic systems and devices for processing, extracting, or purifying one or more ana- lytes. These systems and devices can be used for processing samples and extracting nucleic acids, for example by isotachophoresis. In particular, the systems and related methods can allow for extraction of nucleic acids, including non-crosslinked nucleic acids, from samples such as tissue or cells. The systems and devices can also be used for multiplex parallel sample processing. 604 W O 20 17 / 13 2630 Al