Patent SG-11201809575T-A (SG)
Title: Biocatalysts and methods for hydroxylation of chemical compounds
Inventor: NAZOR JOVANA
IPC: C12P17/12
Filed: 2017-04-19
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111011110111011111111010111110011111111111 VIII11111 H 1111111110 11111111 Organization - ' (10) International Publication Number International Bureau (43) International Publication Date .....0\"\"\" WO 2017/213758 Al 14 December 2017 (14.12.2017) WIP0 I PCT (51) International Patent Classification: (US). ENTWISTLE, David; 200 Penobscot Drive, Red- C12P 17/12 (2006.01) C12P 13/24 (2006.01) wood City, California 94063 (US). VOLADRI, Rama; (21) International Application Number: 200 Penobscot Drive, Redwood City, California 94063 (US). GARCIA, Ravi, David; 200 Penobscot Drive, Red- PCT/US2017/028370 wood City, California 94063 (US). MOORE, Jeffrey, C.; (22) International Filing Date: 367 Orenda Circle, Westfield, New Jersey 07090 (US). 19 April 2017 (19.04.2017) GROSSER, Shane; 249 Fisher Place, Princeton, New Jer- (25) Filing Language: English sey 08540 (US). KOSJEK, Birgit; 680 Summit Avenue, Westfield, New Jersey 07090 (US). TRUPPO, Matthew; (26) Publication Language: English 49 Hillside Terrace, Ocean Township, New Jersey 07712 (30) Priority Data: (US). 62/347,724 09 June 2016 (09.06.2016) US (74) Agent: MACKNIGHT, Kamrin, T.; CODEXIS, INC., 200 Penobscot Drive, Redwood City, California 94063 (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- (US). ye, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every (72) Inventors: NAZOR, Jovana; 200 Penobscot Drive, Red- kind of national protection available): AE, AG, AL, AM, wood City, California 94063 (US). OSBORNE, Robert; AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, 12505 Stonemill Way, Raleigh, North Carolina 27614 (US). CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, LIANG, Jack; 200 Penobscot Drive, Redwood City, Cal-DZ, EC, EE, EG, ES, FE GB, GD, GE, GH, GM, GT, HN, — ifornia 94063 (US). VROOM, Jonathan; 200 Penobscot HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, Drive, Redwood City, California 94063 (US). ZHANG, — KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, = Xiyun; 34279 Maybird Circle, Fremont, California 94555 MK, MN, MW, MX, MY MZ, NA, NG, NI, NO, NZ, OM, = (54) Title: BIOCATALYSTS AND METHODS = = 100 = -T 1 1 1 7 FOR HYDROXYLATION P i F II TIT OF IT 1 CHEMICAL -- T 1 . COMPOUNDS — 1 1 11 1 1 -* -60% Air @ 720 rpm 90 ! = ! 1 il l ! ,2-1-;-*--1-?• Lysine 75%Air 650 -4c- @ rpm = = 7CE = i , ‘ = • 1 414 III - 1 i - 1 80 i 1 ! I - ' ' - ' -, I Lysine -• -60% Air @720 rpm 60+-r- = - I- - 1 ! ! 1 i 1 ! 1 ____ i Pip. Acid = 1 t :1 i i i1 ! -•-75%Air @ 650 rpm —_ 50 fit %of Pox Mix [AUC 250 nm of - l 'Itt = Dansyi Deny.] - - .- i l ' I 11 ::--[--t - , '111 N 1 1 I: ' : ! I Pip. Acid - • - 60% Air @ 720 rpm 40 = = ; ; 11 L 1 1 1 : 1- ' . 1 ; 1, i i - 1 1 ! F i • Product 75%Air 650 30 ; ! # 1 1 k s ! 1 1 I 1 h 1 I I --n-- @ rpm Product = 20 ; -- _ - 4-- t - 1 i I 1 _LA_ ! I! • 1 = 1 t ! x 1 1 ‘, 4 I i i I • 1 1 -. i 1 10 1 _ . f 1 1 xiti 1 1 1 1 1 I --liti-fl '' 0 4 8 12 16 20 24 28 32 36 40 44 48 1-1 [h] GC IN FIG. I IN cr, ,—, ei ----. (57) : The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated com- IN pounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydrox- ylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceu- el tical agents. C [Continued on next page] WO 2017/213758 Al MIDEDIM011111111111111111111111111111111111111 011111111 11111110 111110111111111111 PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a))