Patent SG-11201804699W-A (SG)
Title: Open-top microfluidic device with structural anchors
Inventor: LEVNER DANIEL
IPC: C12M3/00
Filed: 2016-12-02
Abstract: INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau IIIIII Hit II EH DID IIIII DEEM II 11111111111111111111111111111111R1111111 (10) International Publication Number WO 2017/096297 Al WIPO I PCT (43) International Publication Date 8 June 2017 (08.06.2017) (74) Agents: CARROLL, Peter G. et al.; Medlen & Carroll, LLP, 1440 Broadway, Suite 510, Oakland, CA 94612 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art 21(3)) (51) International Patent Classification: C12M 3/00 (2006.01) C12M 3/06 (2006.01) (21) International Application GO1N 33/50 (2006.01) Number: PCT/US2016/064814 (22) International Filing Date: 2 December 2016 (02.12.2016) English English (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/263,492 4 December 2015 (04.12.2015) US (71) Applicant: EMULATE, INC. [US/US]; 27 Drydock, Bo- ston, MA 02110 (US). (72) Inventors: LEVNER, Daniel; 55 Sewall Ave, 5C, Brook- line, MA 02446 (US) HINOJOSA, Chris; 69 Holworthy Street, Cambirdge, MA 02138 (US). WEN, Norman; 4975 Washington Street, Unit 118, West Roxbury, MA 02132 (US). VARONE, Antonio; 865 Boylston Street, Newton, MA 02461 (US). NGUYEN, Justin; 45 Cross St., Apt 24, Medford, MA 02155 (US). WILLIAMSON, Lina; 273 Brown Bear, Chapel Hill, NC 27517 (US). KERNS, Jordan; 7 Heritage Dr, Reading, MA 01867 (US). KARALIS, Katia; 9 Colbourn Crescent #2, Brookline, MA 02445 (US). HAMILTON, Geraldine; 222 Hurley Street, Cambridge, MA 02141 (US). LUCCHESI, Carol; 16 Carroll Ct, Westwood, MA 02090 (US). 131 (54) Title: OPEN-TOP MICROFLUIDIC DEVICE WITH STRUCTURAL ANCHORS 100 117 102 137 134 101 103 127 140 128 W O 20 17 /096 297 Al 102 136 126 118 133 FIG.1 112 (57) : A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall sur - faces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.