Patent EA-002520-B1 (EA)
Title: Isolated antibody and a fragment thereof, methods and a kit for the assessment of lipid peroxidation level in a biological sample, method for diagnosing diseases caused by a lipid peroxidation
Inventor: PARTHASARATHY SAMPATH
IPC: C07K16/44
Filed: 1997-09-19
Abstract: 1. An isolated antibody which binds to an antigen formed by reacting a lipid hydroperoxide with a primary amine but that does not substantially bind to an aldehyde modified protein. 2. The antibody of claim 1, which is a conjugate. 3. The antibody of claim 2, wherein said antibody is labeled with a detectable agent. 4. The antibody of claim 3, wherein the detectable agent selected from the group consisting of a fluorochrome, a radioactive label, biotin, horseradish peroxidase, alkaline phosphatase, 2-galactosidase, or other enzyme. 5. The antibody of claim 1, immobilized on a solid support. 6. The antibody of claim 5, wherein the solid support is a membrane or a coating attached to sticks, beads, cups, or flat packs. 7. The antibody of claim 5, wherein the solid support is selected from a cell culture plate, ELISA plate, tube, and polymeric membrane. 8. The antibody of claim 1, wherein the antigen is the reaction product of linoleic hydroperoxide and a primary amine. 9. The antibody of claim 8, wherein the primary amine is in an amino acid group, such as lysine, in albumin or polylysine, or a small molecular weight compound such as phosphatidylethanolamine. 10. The antibody of claim 1, which is humanized. 11. An antibody fragment according to any of claims 1 to 10. 12. A method for the assessment of the level of lipid peroxidation in a biological sample by identifying an antigen formed by the reaction of of a lipid hydroperoxide with a primary amine, wherein providing binding the sample to an antibody or a fragment thereof according to any of claims 1 to 11 and determining the presence of antigen-antibody complex, the level of lipid peroxidation is evaluated according to an amount of the antigen-antibody complex. 13. A method for the assessment of the level of lipid peroxidation in a biological sample by identifying an antigen formed by the reaction of of a lipid hydroperoxide with a primary amine, wherein providing contacting the sample with an antibody or a fragment thereof or a conjugate thereof that is crossreactive with an antibody or a fragment thereof according to any of claims 1 to 11 and determining the presence of antigen-antibody complex, the level of lipid peroxidation is evaluated according to an amount of the antigen-antibody complex. 14. The method of claim 13, wherein the level of lipid peroxidation is compared to a population norm. 15. A kit for the assessment of lipid peroxidation in a biological sample, comprising an antibody or a fragment thereof according to any of claims 1 to 11. 16. The kit according to claim 14, further comprising a second antibody reacting with an antibody of a fragment thereof according to any of claims 1 to 11. 17. A method for diagnosing diseases caused by a lipid peroxidation, comprising assessing the level of a lipid peroxidation in a biolological sample according to claims 12-13. 18. The method according to claim 16, wherein a disease is selected from the group consisting of cardiovascular disease, atherosclerosis, inflammatory disease, endometriosis, glymerol nephritis, preeclampsia, central nervous system disorders mediated by lipid peroxidation, Alzheimer's disease, psoriasis, asthma, atopic dermatitis, solid tumors, Kaposi's sarcoma, neurodegenerative disease, inflammatory bowel disease (Crohn's disease), rheumatoid arthritis, and ischemia reperfusion.