Patent AU-2013204253-B2 (AU)
Title: Flow cells for high density array chips
Inventor: PECK BILL J
IPC: C12Q1/68
Filed: 2013-03-22
Granted: 2016-09-29
Abstract: (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2013/148525 Al 3 October 2013 (03.10.2013) W I PO I P CT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every BOIL 3/00 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, PCT/US2013/033583 DO, DZ, EC, EE, EG, ES, Fl, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, 22 March 2013 (22.03.2013) KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, (25) Filing Language: English NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, (26) Publication Language: English RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, (30) Priority Data: ZM, ZW. 61/617,628 29 March 2012 (29.03.2012) US 13/840,482 15 March 2013 (15.03.2013) US (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: COMPLETE GENOMICS, INC. [US/US]; GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, 2071 Stierlin Court, Mountain View, California 94043 UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (US). TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (72) Inventors: PECK, Bill J.; 2071 Stierlin Court, Mountain EE, ES, Fl, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, View, California 94043 (US). FULLER, Mark; 2071 Sti- MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, berlin Court, Mountain View, California 94043 (US). TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, WEST, Daniel; 2071 Stierlin Court, Mountain View, Cali- ML, MR, NE, SN, TD, TG). fornia 94043 (US). DELACRUZ, Anthony; 2071 Stierlin Published: Court, Mountain View, California 94043 (US). - with international search report (Art. 21(3)) (74) Agents: ALLEN, Kenneth R. et al.; Kilpatrick Townsend & Stockton LLP, Two Embarcadero Center, 8th Floor, San Francisco, California 94111-3834 (US). (54) Title: FLOW CELLS FOR HIGH DENSITY ARRAY CHIPS (57) Abstract: Biochemical flow cells having sealed inlets and outlets are provided for per 110 forming high-volume assays on macromolecules. A flow cell with detachable inlet and outlet con nectors comprises an inlet manifold, a coverslip, and a substrate disposed below the coverslip to form a reaction chamber, where the substrate is disposed to partially cover the inlet manifold 1.o such that a slit is formed along an entire edge of 137 the substrate where fluids can flow from the inlet 52 manifold through the slit, around substantially s the entire edge of the substrate, and into the reac 1- tion chamber at equalized pressure and without 10s 147 bubbles. In another embodiment, the flow cell 14s comprises an outlet manifold, two or more flow regions each connected to its own loading port via its own flow distribution funnel, each loading 129 port connected to the outlet manifold, and plugs /\15 in a wall of the outlet manifold opposite each 100 127 loading port. It25 Fig. 1