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Reversible, reproducible reduction of brain water apparent diffusion coefficient by cortical electroshocks. | ation of electroshock trains. ADC reduction was reproducible in extent and time course after single 10-shock trains and was reversible. In the affected pixels the mean ADC reduction was 4% for a single shock train (0.1 s); and 7-8% for trains repeated once a minute; independent of electroshock train duration. The results indicate that neuronal activity associated with electrostimulation may be monitored with water diffusion measurements; and they may be useful for measuring the severity of seizure activity | 8978625 | [
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Reversible, reproducible reduction of brain water apparent diffusion coefficient by cortical electroshocks. | in patients with medically intractable epilepsy. | 8978625 | [
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Characterization and prediction of gradient acoustic noise in MR imagers. | Gradient acoustic noise has been measured and characterized for an epoxy-potted; shielded gradient assembly in a 1.5 T MRI system. Noise levels vary by 10 dB or more as a function of longitudinal position in the scanner and reflect the pattern of forces applied to the gradient assembly. The noise level increases slightly (1-3 dB) with a patient in the scanner. The spectrum of the noise is similar (but not identical) to the spectrum of the input signal. A gradient-pulse-to-acoustic-noise transfer function wa | 8978626 | [
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Characterization and prediction of gradient acoustic noise in MR imagers. | s obtained by using a white noise voltage input to the gradient system. The transfer function enabled us to accurately predict acoustic noise output for a pulse sequence consisting of a series of trapezoidal pulses on a single axis and for a clinical fast spin echo sequence with gradients present on all three axes. | 8978626 | [
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Multinuclear solid-state NMR spectroscopy of envelopes from virgin and explanted silicone breast prostheses: an exploratory study. | The structure and dynamics of silicone envelopes from virgin and explanted breast prostheses have been studied by 1H; 13C; and 29Si high-resolution solid-state NMR spectroscopy. The results indicate that this combination of techniques can be used to unambiguously identify the presence of methyltrifluoropropylsiloxane units and diphenylsiloxane units at concentrations of about 2.0% and 3.5%; respectively; in some of the envelopes. The presence of about 10% silica also was detected and a trace of lipids was f | 8978627 | [
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Multinuclear solid-state NMR spectroscopy of envelopes from virgin and explanted silicone breast prostheses: an exploratory study. | ound in the explanted silicone envelopes. We have also measured the proton T2 and T1 relaxation times of the envelopes to characterize the dynamics of the silicone in the envelopes. | 8978627 | [
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Temporal lobe epilepsy: correlation of proton magnetic resonance spectroscopy and 18F-fluorodeoxyglucose positron emission tomography. | Proton magnetic resonance spectroscopy (MRS) has demonstrated reduction of N-acetylaspartate (NAA) in the epileptogenic temporal lobe. However; the correlation of NAA reduction with cerebral metabolic abnormalities is unknown in temporal lobe epilepsy (TLE). Proton MRS and 18F-fluorodeoxyglucose positron emission tomography (FDG/PET) were used to study 12 unilateral TLE patients with medically intractable seizures and 26 age-matched healthy volunteers. The epileptogenic temporal lobe of each patient was det | 8978628 | [
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Temporal lobe epilepsy: correlation of proton magnetic resonance spectroscopy and 18F-fluorodeoxyglucose positron emission tomography. | ermined by both electroencephalography and FDG/PET. The NAA/choline-plus-creatine (NAA/(Cho+Cr)) ratio correlated significantly with the interictal glucose metabolism (r = 0.54; P < 0.01) in 12 TLE patients. The mean NAA/(Cho+Cr) ratio in the epileptogenic temporal lobe was significantly less than that in the contralateral side (P < 0.01); and less than that in normal control temporal lobes (P < 0.0001). These results suggest that quantitative MRS abnormalities reflect underlying metabolic pathology in TLE. | 8978628 | [
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Histological and 1H magnetic resonance spectroscopic imaging analysis of quinolinic acid-induced damage to the rat striatum. | NAA has been described as a neuron-specific compound. NAA levels as determined by magnetic resonance spectroscopic imaging (MRSI) have been used to determine degree of neuronal loss in several neurological diseases; but there has been limited work to document the accuracy and reliability of this technique. This study addresses this question quantitatively with histological analysis of cell viability and tissue shrinkage in quinolinic acid (QA)-induced damage of the rat striatum compared with 1H MRSI measure | 8978629 | [
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Histological and 1H magnetic resonance spectroscopic imaging analysis of quinolinic acid-induced damage to the rat striatum. | ment of N-acetyl aspartate (NAA) as a noninvasive measure of neuronal loss. Both 1H MRSI and histology detect damage to the lesioned striatum; however; there are differences in the degree of damage as assessed by the two methods. Although partial-volume effects and tissue shrinkage may decrease the sensitivity of MR to such damage; the sparing of axons by QA may be another important factor in the differences in assessment. These results indicate that further studies of NAA metabolism and its distribution wi | 8978629 | [
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Histological and 1H magnetic resonance spectroscopic imaging analysis of quinolinic acid-induced damage to the rat striatum. | thin neurons are warranted. | 8978629 | [
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In vivo measurement of T2 distributions and water contents in normal human brain. | Using a 32-echo imaging pulse sequence; T2 relaxation decay curves were acquired from five white- and six gray-matter brain structures outlined in 12 normal volunteers. The water contents of white and gray matter were 0.71 (0.01) and 0.83 (0.03) g/ml; respectively. All white-matter structures had significantly higher myelin water percentages (signal percentage with T2 between 10 and 50 ms) than all gray-matter structures. The range in geometric mean T2 of the main peak for both white and gray matter was fro | 8978630 | [
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In vivo measurement of T2 distributions and water contents in normal human brain. | m 70 to 86 ms. T2 distributions from the posterior internal capsules and splenium of the corpus callosum were significantly wider (width is related to water environment inhomogeneity) than those from any other white- or gray-matter structures. Thus; quantitative measurement and analysis of T2 relaxation reveals differences in brain tissue water environments not discernible on conventional MR images. These differences may make short T2 components reliable markers for normal myelin. | 8978630 | [
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] |
T1rho of protein solutions at very low fields: dependence on molecular weight, concentration, and structure. | The effect of molecular weight; concentration; and structure on 1/T1rho; the rotating frame relaxation rate; was investigated for several proteins using the on-resonance spin-lock technique; for locking fields B1 < 200 microT. The measured values of 1/T1rho were fitted to a simple theoretical model to obtain the dispersion curves 1/T1rho(omega1) and the relaxation rate at zero B1 field; 1/T1rho(0). 1/T1rho was highly sensitive to the molecular weight; concentration; and structure of the protein. The amount | 8978632 | [
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T1rho of protein solutions at very low fields: dependence on molecular weight, concentration, and structure. | of intra- and intermolecular hydrogen and disulfide bonds especially contributed to 1/T1rho. In all samples; 1/T1rho(0) was equal to 1/T2 measured at the main magnetic field Bo = 0.1 T; but at higher locking fields the dispersion curves monotonically decreased. The results of this work indicate that a model considering the effective correlation time of molecular motions as the main determinant for T1rho relaxation in protein solutions is not valid at very low B1 fields. The underlying mechanism for the rela | 8978632 | [
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T1rho of protein solutions at very low fields: dependence on molecular weight, concentration, and structure. | xation rate 1/T1rho at B1 fields below 200 microT is discussed. | 8978632 | [
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Diffusion of solutes in agarose and alginate gels: 1H and 23Na PFGSE and 23Na TQF NMR studies. | Cells immobilized in gels experience potential metabolic restrictions in the form of reduced diffusion rates of metabolites and ions and their possible selective adsorption on the gel matrix. Diffusion and relaxation characteristics of common solutes in agarose and barium alginate gels were investigated at 37 degrees C by using 1H PFGSE and 23Na TQF NMR spectroscopy. Glucose; glycine; alanine; lactate; sodium ions; and HDO were studied. There were no selective interactions between any of the metabolites and | 8978631 | [
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Diffusion of solutes in agarose and alginate gels: 1H and 23Na PFGSE and 23Na TQF NMR studies. | the gel materials but the diffusion coefficients were uniformly reduced. The effects of metabolite diffusion and utilization; in gel beads and threads containing cells; were simulated by using a reaction diffusion model incorporating the measured diffusion coefficients. Metabolism is expected to be very significantly limited by diffusion of solutes to and from the cells that are centrally located within gel threads or spheres of radius approximately 2.0 mm; which is a commonly used size. | 8978631 | [
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Estimation of water extraction fractions in rat brain using magnetic resonance measurement of perfusion with arterial spin labeling. | The model used for calculating perfusion by MRI techniques that use endogenous water as a tracer assumes that arterial water is a freely diffusible tracer. Evidence shows that this assumption is not valid in the brain at high blood flow rates; at which movement of water into and out of the microvasculature becomes limited by diffusion across the blood-brain barrier. In this work; the arterial spin-labeling technique is used to show that fraction of arterial water that is dependent on blood flow rate remains | 8978633 | [
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Estimation of water extraction fractions in rat brain using magnetic resonance measurement of perfusion with arterial spin labeling. | in the vasculature and does not exchange with brain tissue water. By using perfusion measurements without and with magnetization transfer (MT) effects; one can distinguish arterial label that exchanges into tissue because blood has much smaller MT than brain tissue. Using this technique; the extraction fraction for water is measured in the rat brain at various cerebral blood flow rates. At high flow rates (approximately 5 ml/g/min); the extraction fraction for water is found to be about 45% in rat brain. D | 8978633 | [
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Estimation of water extraction fractions in rat brain using magnetic resonance measurement of perfusion with arterial spin labeling. | isruption of the blood-brain barrier with D-mannitol caused an increase in the extraction fraction for water. It was possible to form an image related to the extraction fraction for water. The ability to estimate the amount of vascular water exchanging with tissue water by MRI may represent a noninvasive approach to detect the integrity of the blood-brain barrier. | 8978633 | [
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Measurement of strain exerted on blood vessel walls by double-quantum-filtered 2H NMR. | In this study 2H double quantum-filtered (DQF) NMR spectroscopy of water molecules inside the blood vessel wall was used to monitor structural changes after the application of strain. This method was applied to a variety of bovine large blood vessels; including aorta; coronary artery; carotid artery; and vena cava. Inducing strain in coronary arteries by inflating them by air or water revealed dramatic changes in the 2H DQF spectral lineshapes. Uniaxial extensions of longitudinal and circumferential strips | 8978634 | [
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Measurement of strain exerted on blood vessel walls by double-quantum-filtered 2H NMR. | led to distinct spectral responses for the different blood vessels. While the spectral lineshapes for the coronary and the carotid arteries were very sensitive to longitudinal strain; they were not affected by circumferential stretching. For the aorta there were no changes in the lineshape upon strain. For the vena cava a large effect of strain was found that was independent of the choice of elongation axis. The effect of elongation on the spectra was assigned to the outer layer; the adventitia; whereas spe | 8978634 | [
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Measurement of strain exerted on blood vessel walls by double-quantum-filtered 2H NMR. | ctral lineshapes that originated from inner layers; media and intima; were almost insensitive to strain. Angular dependence experiments proved the presence of macroscopic order in the strained state superimposed on a broad distribution of the local directors. Our results demonstrate the sensitivity of 2H DQF NMR as a probe for dynamic processes occurring on the microscopic scale in a molecular network. | 8978634 | [
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Unsupervised, automated segmentation of the normal brain using a multispectral relaxometric magnetic resonance approach. | The purpose of this study was the development and testing of a method for unsupervised; automated brain segmentation. Two spin-echo sequences were used to obtain relaxation rates and proton-density maps from 1.5 T MR studies; with two axial data sets including the entire brain. Fifty normal subjects (age range; 16 to 76 years) were studied. A Three-dimensional (3D) spectrum of the tissue voxels was used for automatic segmentation of gray matter (GM); white matter (WM); and cerebrospinal fluid (CSF) and for | 8978636 | [
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Unsupervised, automated segmentation of the normal brain using a multispectral relaxometric magnetic resonance approach. | calculation of their volumes. Accuracy and reproducibility were tested with a three-compartment phantom simulating GM; WM; and CSF. In the normal subjects; a significant decrease of GM fractional volume and increased CSF volume with age were observed (P < 0.0001); with no significant changes in WM. This multispectral segmentation method permits reproducible; operator-independent volumetric measurements. | 8978636 | [
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An FEM approach for the characterization of the RF field homogeneity at high field. | High field magnetic resonance offers new opportunities because of its high SNR and better spectral resolution for MRI and MRS. However; new problems also emerge at high field. As the field strength increases; the wavelength in the tissue becomes shorter and comparable with the body dimensions. This perturbs the field and also causes standing waves within the patient as a result of the impedance mismatching at the tissue interfaces. Due to the complexity of the boundary conditions and the solution of Maxwell | 8978635 | [
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An FEM approach for the characterization of the RF field homogeneity at high field. | 's equations; an exact analytical calculation for a loaded RF resonator has not been possible. In this paper; we present a birdcage coil simulation study based on a 3D finite element method (FEM) model for the characterization of the field within the tissue. First; the accuracy of the FEM solutions is validated by the 2D analytical solutions at 64 and 223 MHz. In these solutions; the frequency dependence of the conductivity and permittivity is also taken into account. Then; a more realistic 3D model is stud | 8978635 | [
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An FEM approach for the characterization of the RF field homogeneity at high field. | ied. The results are compared with the experimental measurements. It is shown that the 3D model makes it possible to explore the effects of the end rings in the presence of a tissue sample inside the coil. | 8978635 | [
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A dual approach for minimizing false lesion classifications on magnetic resonance images. | Segmentation methods based on dual-echo MR images are generally prone to significant false lesion classifications. We have minimized these false classifications by (1) improving the lesion-to-tissue contrast on MR images by developing a fast spin-echo sequence that incorporates both cerebrospinal fluid signal attenuation and magnetization transfer contrast and (2) including information from MR flow images. Studies on patients with multiple sclerosis indicate that this dual approach to tissue segmentation re | 8978637 | [
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A dual approach for minimizing false lesion classifications on magnetic resonance images. | duces the volume of false lesion classifications by an average of 87%. | 8978637 | [
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An analytical model of restricted diffusion in bovine optic nerve. | An analytical model of restricted diffusion in bovine optic nerve is presented. The nerve tissue model is composed of two different objects: prolate ellipsoids (axons) and spheres (glial cells) surrounded by partially permeable membranes. The free diffusion coefficients of intracellular and extracellular water may differ. Analytical formulas for signal loss due to diffusion in the pulsed gradient spin echo (PGSE) experiment for this tissue model are derived. The model is fitted to experimental data for bovi | 8978638 | [
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An analytical model of restricted diffusion in bovine optic nerve. | ne optic nerve. The obtained model parameters are shown to be reasonable. The model describes all of the characteristics of the PGSE data: anisotropy; upward curvature of decay curves; and diffusion time dependence. The validity and sensitivity of the model are also discussed. | 8978638 | [
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Intravascular magnetic resonance imaging using a loopless catheter antenna. | Recently; intravascular catheter probes have been developed to increase signal-to-noise ratio (SNR) for MR imaging of blood vessels. Miniaturization of these catheter probes without degrading their performances is very critical in imaging small vessels such as coronary arteries. Catheter coils have a loop incorporated in their structure and have limitations in physical dimensions and electromagnetic properties. The use of a loopless intravascular catheter antenna is proposed to overcome these problems. The | 8978639 | [
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Intravascular magnetic resonance imaging using a loopless catheter antenna. | catheter antenna is essentially a dipole; which makes a very thin diameter possible; and its electronic circuitry can be placed outside the blood vessels without performance degradation. The theoretical foundation for the design and operation of the catheter antenna is presented. Several catheter antennae; as small as 1.5 French; were constructed and tested on phantoms and rabbits with great success. The catheter antenna has a simple structure and is easy to design; implement; and operate. | 8978639 | [
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Cerebrospinal fluid-suppressed high-resolution diffusion imaging of human brain. | A cerebrospinal fluid (CSF)-suppressed flow-attenuated inversion recovery (FLAIR) double-shot diffusion echo-planar imaging (EPI) sequence was developed and used; along with a non-CSF-suppressed version of the sequence; to determine the extent of the contribution of CSF partial-volume averaging to the apparent diffusion coefficients (ADCs) of normal human brain in vivo. Regional analysis indicates that cortical gray matter and parenchymal tissues bordering the ventricles are most affected by CSF contaminati | 8978640 | [
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Cerebrospinal fluid-suppressed high-resolution diffusion imaging of human brain. | on; leading to elevated ADC values. Only slight differences in gray- and white-matter average ADCs were detected after CSF suppression. The human brain average ADCs calculated from high-resolution CSF-suppressed diffusion-weighted images in these studies are similar to those reported in animals. FLAIR diffusion sequences remove CSF as a source of error in ADC determination and ischemic lesion discrimination in diffusion-weighted images (DWI) and ADC maps. | 8978640 | [
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] |
Techniques for high-speed cardiac magnetic resonance imaging in rats and rabbits. | Progress in research on hypertension; heart failure; aging; post-infarct remodeling; and the molecular basis of cardiovascular diseases in general has been greatly facilitated in recent years by the development of specialized small-mammal models by selective breeding and/or genetic alteration. Routine noninvasive evaluation of cardiac function and perfusion in these animals models; however; is difficult using existing methods. In principle; MRI can be used for this purpose; but in practice this is difficult | 8978641 | [
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Techniques for high-speed cardiac magnetic resonance imaging in rats and rabbits. | because of problems related to RF coils; cardiac gating; and imaging pulse sequences. In this article; solutions to these problems are described that have allowed us to use MRI to routinely image the hearts of rats and rabbits. Specifically described are four RF coils; cardiac gating schemes; and an imaging pulse sequence specially designed for cardiac imaging in these animals on a 4.7 T Omega chemical-shift imaging (CSI) spectrometer. These techniques can be used to obtain; within 2 min; eight double-obli | 8978641 | [
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Techniques for high-speed cardiac magnetic resonance imaging in rats and rabbits. | que short-axis images of the rat at different cardiac phases with 200 x 400 microm in-plane resolution and a slice thickness of 2 mm. Moreover; myocardial tissue tagging can be performed with tag thicknesses and separations comparable to those used routinely in humans. The technical information is presented in sufficient detail to allow researchers at other sites to reproduce the results. This information should facilitate the use of MRI for the noninvasive examination of cardiac function and perfusion; whi | 8978641 | [
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Techniques for high-speed cardiac magnetic resonance imaging in rats and rabbits. | ch can be combined with other established techniques for the study of cardiovascular disease in specialized animal models. | 8978641 | [
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Decoupled automated rotational and translational registration for functional MRI time series data: the DART registration algorithm. | A rapid; in-plane image registration algorithm that accurately estimates and corrects for rotational and translational motion is described. This automated; one-pass method achieves its computational efficiency by decoupling the estimation of rotation and translation; allowing the application of rapid cross-correlation and cross-spectrum techniques for the determination of displacement parameters. k-space regridding and modulation techniques are used for image correction as alternatives to linear interpolati | 8978642 | [
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Decoupled automated rotational and translational registration for functional MRI time series data: the DART registration algorithm. | on. The performance of this method was analyzed with simulations and echo-planar image data from both phantoms and human subjects. The processing time for image registration on a Hewlett-Packard 735/125 is 7.5 s for a 128 x 128 pixel image and 1.7 s for a 64 x 64 pixel image. Imaging phantom data demonstrate the accuracy of the method (mean rotational error; -0.09 degrees; standard deviation = 0.17 degrees; range; -0.44 degrees to +0.31 degrees; mean translational error = -0.035 pixels; standard deviation = | 8978642 | [
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Decoupled automated rotational and translational registration for functional MRI time series data: the DART registration algorithm. | 0.054 pixels; range; -0.16 to +0.06 pixels). Registered human functional imaging data demonstrate a significant reduction in motion artifacts such as linear trends in pixel time series and activation artifacts due to stimulus-correlated motion. The advantages of this technique are its noniterative one-pass nature; the reduction in image degradation as compared to previous methods; and the speed of computation. | 8978642 | [
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Normal myocardial perfusion assessed with multishot echo-planar imaging. | A new magnetic resonance imaging strategy is presented for accessing myocardial perfusion. Most previous work has relied on using T1-weighted fast gradient-echo imaging to monitor dynamically the signal changes during the passage of a contrast media bolus. However; the gradient-echo approach is limited by an inability to image the entire heart with adequate temporal resolution. This paper focuses on a electrocardiogram-gated multishot echo-planar imaging sequence; using the simple strategy of using the intr | 8978643 | [
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Normal myocardial perfusion assessed with multishot echo-planar imaging. | insic T1 weighting produced by a repetition time equal to the heart period. To quantitate the sequence's performance with respect to normal myocardial perfusion; seven volunteers were imaged; each with three different doses of the contrast medium gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). The first-pass dynamics of the contrast were quantified in 13 regions per heart for each examination. In all volunteers; the complete heart could be covered; with five to seven slices; every two heartbeats. | 8978643 | [
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Normal myocardial perfusion assessed with multishot echo-planar imaging. | Enhancement was homogeneous throughout the left ventricular myocardium; with an enhancement of approximately 50% for the optimum contrast dose of 0.05 mmol/kg Gd-DTPA. | 8978643 | [
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Prospective adaptive navigator correction for breath-hold MR coronary angiography. | Current MR coronary angiography (MRCA) methods use breath-holding to minimize respiratory motion. A major limitation to this technique is misregistration between imaging slices due to breath-hold variability. Prospective adaptive correction of image location using real-time navigator measurement of diaphragm position is a potential method for improving slice registration in breath-hold MRCA. Ten subjects underwent MRCA using an ECG-gated; fat-suppressed; segmented k-space; gradient-echo sequence. Transverse | 8978644 | [
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Prospective adaptive navigator correction for breath-hold MR coronary angiography. | and coronal images were acquired using standard breath-holding with and without prospective navigator correction. Breath-hold MRCA with prospective navigator correction resulted in a 47% reduction in craniocaudal slice registration error compared to standard breath-holding (0.9 +/- 0.2 mm versus 1.7 +/- 0.4 mm; P = 0.04). Prospective adaptive navigator correction of image location significantly improves slice registration for breath-hold MRCA and is a promising motion correction technique for cardiac MR. | 8978644 | [
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Magnetization and diffusion effects in NMR imaging of hyperpolarized substances. | The special magnetization characteristics of hyperpolarized noble gases have led to an interest in using these agents for new MRI applications. In this note; the magnetization effects and NMR signal dependence of two noble gases; 3He and l29Xe; are modeled across a range of gradient-echo imaging parameters. Pulse-sequence analysis shows a wide variation in optimum flip angles between imaging of gas (e.g.; 3He or 129Xe) in air spaces (e.g.; trachea and lung) and in blood vessels. To optimize imaging of the a | 8978645 | [
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Magnetization and diffusion effects in NMR imaging of hyperpolarized substances. | ir spaces; it is also necessary to reduce the otherwise substantial signal losses from diffusion effects by increasing voxel size. The possibility of using hyperpolarized 129Xe for functional MRI (fMRI) is discussed in view of the results from the blood flow analysis. The short-lived nature of the hyperpolarization opens up new possibilities; as well as new technical challenges; in its potential application as a blood-flow tracer. | 8978645 | [
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Effects of aging on titanium implants inserted into the tibiae of female rats using light microscopy, SEM, and image processing. | We examined the influence of aging on the implant-bone interface of titanium implants inserted transcortically and extending into the medullary canal of rat tibiae; and quantitatively assessed the differences in bone reaction using an image processing system. Three groups of 15 female rats; aged 6 weeks (young group); 22 weeks (adult group); and 80 weeks (old group) were used in this experiment. The animals were sacrificed 28 days after implant placement. Toluidine blue stained undecalcified sections were p | 8978646 | [
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Effects of aging on titanium implants inserted into the tibiae of female rats using light microscopy, SEM, and image processing. | repared for histological observation and image analysis; and the implant socket was observed by SEM. There was no difference in the degree of maturation of newly formed bone between the young and adult groups. Titanium implants inserted in the young and adult groups were surrounded with a bone layer. In the old group; however; there was little mature bone tissue around the implants. Quantitative evaluation indicated that the young group showed the highest; the adult group showed a slightly lower; and the ol | 8978646 | [
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Effects of aging on titanium implants inserted into the tibiae of female rats using light microscopy, SEM, and image processing. | d group showed the lowest percent bone contact; thickness of bone contact; and area of bone surrounding the implant. | 8978646 | [
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Fretting corrosion resistance and fretting corrosion product cytocompatibility of ferritic stainless steel. | To avoid nickel ion release from SUS317L as an implant material; a new type of nickel; commercially free; of high purity; and high chromium ferritic stainless steel; was developed. The new stainless steel (FJ) was studied for aspects of fretting corrosion and cytocompatibility compared with SUS317L. A pin-on-plate fretting corrosion test in an artificial physiologic solution; and cell culture in media with the addition of the artificial physiologic solution used for fretting was conducted. Resistance to the | 8978647 | [
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Fretting corrosion resistance and fretting corrosion product cytocompatibility of ferritic stainless steel. | fretting induced crevice corrosion of FJ was higher than that of SUS317L because of the favorable electrochemical stability of the FJ alloy. The amount of iron ion or colloidal fine particles released from FJ was about a quarter of that from SUS317L; although the weight loss of a pin of FJ was almost 5/3 that of SUS317L. The artificial physiologic solution used for SUS317L fretting was more harmful to the growth of L929 and MC3T3-E1 cells than that used for FJ fretting. FJ was therefore superior to SUS317L | 8978647 | [
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Effect of atmosphere on phase transformation in plasma-sprayed hydroxyapatite coatings during heat treatment. | The purpose of this study was to evaluate the effect of atmosphere on the phase transformation in hydroxyapatite (HA) coatings during heat treatment by varying the atmosphere in the furnace pipe. Heat treatment always increased the crystallinity of HA coatings regardless of the kind of atmosphere. Water molecules in atmosphere further promoted HA recrystallization during heat treatment. In a dry atmosphere; tricalcium phosphate (TCP) and tetracalcium phosphate (TTCP) were more stable than HA; so heat treatm | 8978648 | [
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Effect of atmosphere on phase transformation in plasma-sprayed hydroxyapatite coatings during heat treatment. | ent could not convert them into HA. However; in a humid atmosphere; heat treatment would transform TCP and TTCP into HA by hydrolytic reactions. | 8978648 | [
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Use of chitosan as a biomaterial: studies on its safety and hemostatic potential. | Chitosan; a mucopolysaccharide of marine origin; was studied for its safety and hemostatic potential. Its surface was treated with glutaraldehyde; carbodiimide; and plasma glow discharge to elicit effects of enzyme degradation. Of the seven enzymes used; leucine amino peptidase caused maximum degradation. Autoclaving appeared to be an ideal sterilizing method as it caused least decrease in tensile strength and effected a negligible rate of hemolysis. Sterilizing with glutaraldehyde with a physiologic pH ret | 8978649 | [
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Use of chitosan as a biomaterial: studies on its safety and hemostatic potential. | ained the maximum tensile strength of chitosan. In vivo toxicity tests indicated that it is nontoxic; and the sterilized films were free of pyrogen. Coagulation and hemagglutination tests showed that the hemostatic mechanism of chitosan seems to be independent of the classical coagulation cascade and appears to be an interaction between the cell membrane of erythrocytes and chitosan. | 8978649 | [
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Effects of metal ions on osteoblast-like cell metabolism and differentiation. | The objective of this study was to evaluate the effects of metal ions; which may be released from orthopedic or dental implants; on osteoblast metabolism and differentiation. ROS 17/2.8 cells were cultured in F-12 medium for 7 days. Then Al+3; Co+2; Cr+3; Ni+2; Ti+4; and V+3 were added at concentrations less than their cytotoxic concentrations. After 3 days; DNA synthesis; succinate dehydrogenase activity; alkaline phosphatase (ALP) activity; and culture calcification were assessed. Northern blots were perf | 8978650 | [
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Effects of metal ions on osteoblast-like cell metabolism and differentiation. | ormed for ALP; osteocalcin (OCN); and osteopontin (OPN) mRNA transcription. The data indicated that Cr+3 and A1+3 had few inhibitory effects on ROS cell metabolism below their cytotoxic concentrations; Ni+2; Co+2; Ti+4; and V+3 affected all these parameters of ROS cell metabolism at concentrations below cytotoxic levels. For RNA analysis; A1+3 significantly suppressed the expression of ALP; OCN; and OPN at both cytotoxic and noncytoxic concentrations. Co+2 specifically suppressed ALP expression at cytotoxic | 8978650 | [
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Effects of metal ions on osteoblast-like cell metabolism and differentiation. | concentrations. Cr+3 and Ni+2 inhibited OCN; OPN; and ALP gene expression only at cytotoxic concentrations. For Ti+4 and V+3 ions; gene expression at cytotoxic levels was not significantly affected as compared with the effects at noncytotoxic level. These results show that metal ions may alter osteoblast behavior even at subtoxic concentrations; but do not always affect the expression of all genes similarly. | 8978650 | [
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Properties of epsilon-caprolactone/DL-lactide (epsilon-CL/DL-LA) copolymers with a minor epsilon-CL content. | In this study the properties of DL-lactide (DL-LA) copolymers with 5; 10; 15; 20; and 30 wt % (in feed) of epsilon-caprolactone (epsilon-CL) polymerized with stannous(II)octoate (SnOct) as catalyst and glycerol; laurylalcohol; or pentaerythritol as initiator were investigated. Thermal studies showed that the addition of 5 wt % (in feed) of epsilon-CL to the P(CL/DL-LA) copolymer decreased the Tg by about 5 degrees C. Hydrolysis tests were carried out for copolymers with 20 and 30 wt % (in feed) of epsilon-C | 8978651 | [
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Properties of epsilon-caprolactone/DL-lactide (epsilon-CL/DL-LA) copolymers with a minor epsilon-CL content. | L to study the degradation rate. Molecular weights decreased dramatically during the first week of hydrolysis; with mass losses occuring a few weeks later. The influence of glycerol and pentaerythritol as initiators; and the influence of epsilon-CL content on stress-strain behavior; tension set; and rheologic properties of the P(CL/DL-LA) copolymers were also investigated. The tensile testing of P(CL/DL-LA) copolymers containing 5; 10; 15; 20 wt % (in feed) of epsilon-CL showed that the properties of copoly | 8978651 | [
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Properties of epsilon-caprolactone/DL-lactide (epsilon-CL/DL-LA) copolymers with a minor epsilon-CL content. | mers varied from hard and brittle to rubbery. The permanence of elastic properties was investigated with tension set measurements. These studies showed that copolymers crept remarkably under stress. The viscosity and elasticity of P(CL/DL-LA) copolymers at 120 degrees C were investigated using rheology studies. | 8978651 | [
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Investigation into the release of bioactive recombinant human growth hormone from normal and low-viscosity poly(methylmethacrylate) bone cements. | Previous studies showed that recombinant human growth hormone (hGH) released from hormone-loaded poly(methylmethacrylate) (PMMA) cement stimulated osteoid formation in a rabbit model. Local delivery of hGH from cemented hip arthroplasties may thereby provide a means of reducing the problem of aseptic loosening. We have investigated two different formulations of PMMA as delivery systems for bioactive hGH. The bioactivity of the hormone release in vitro was monitored with an eluted stain assay (ESTA). The hGH | 8978652 | [
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Investigation into the release of bioactive recombinant human growth hormone from normal and low-viscosity poly(methylmethacrylate) bone cements. | was also measured by an immunoassay; which provides an alternative assessment of structural integrity of the hormone released. In addition; we adapted the ESTA bioassay to assess the in vitro cytotoxicity of the cements. Using unloaded cements; the undiluted eluates from both types of PMMA proved cytotoxic. This cytotoxicity could be diluted out; and the procedure allowed us to measure the bioactivity of hGH in the eluates from hormone-loaded cements independent of their cytotoxicity. The major fraction of | 8978652 | [
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Investigation into the release of bioactive recombinant human growth hormone from normal and low-viscosity poly(methylmethacrylate) bone cements. | the bioactivity was released from both of the PMMA cements during the first 24 h; but the hormone remained detectable in eluates collected after 36 days of elution. Comparison of the bio- and immunoactivity of the hGH released showed that the ratio of these two activities (i.e.; the B:I ratio) was constant over this time period. However in parallel studies in which hormone-loaded discs were stored under dry conditions prior to elution; we found that the B:I ratio then declined markedly. This suggests that | 8978652 | [
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Investigation into the release of bioactive recombinant human growth hormone from normal and low-viscosity poly(methylmethacrylate) bone cements. | fully hydrated conditions; such as when the discs are bathed in assay medium; are necessary to maintain the bioactivity of the hGH. Both cements released only approximately 1% of the hormone originally incorporated; but the hGH concentration which accumulated in the eluates were high in physiologic terms (approximately 1000 mU/L). | 8978652 | [
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Separation and characterization of polyethylene wear debris from synovial fluid and tissue samples of revised knee replacements. | A study was made of in vivo-generated polyethylene wear particles as separated from synovial fluid samples and from tissue samples surrounding total knee arthroplasty. A comparison of particle size and morphology between the two particle groups was made to assess any effects of selective tissue capture; and macrophage encapsulation and digestion. In addition; a Raman spectroscopy technique was evaluated that enables positive identification of individual wear particles. The particles of the same size range f | 8978653 | [
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Separation and characterization of polyethylene wear debris from synovial fluid and tissue samples of revised knee replacements. | ound in the synovial fluid and tissue samples exhibited a comparable morphology. Notably; submicron-sized debris was present in both the synovial fluid and tissue samples surrounding knees with osteolysis. The novel micro-Raman analysis of individual particles was successful in the categorizing of wear debris as polyethylene or nonpolyethylene. | 8978653 | [
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Maturation-dependent repair of untreated osteochondral defects in the rabbit knee joint. | Repair of untreated full-thickness cartilage defects in the medial femoral knee condyle was studied in 17 young; 21 adolescent; and 19 adult rabbits. At 6 weeks; the defect was completely filled by tissue regeneration in all young and adolescent animals; but in only few of the adults (p < 0.05). The morphology of all repairs improved from fibrous tissue to hyaline-like cartilage over time (p < 0.05); but at 3 and 6 weeks; the repair tissue in young and adolescent rabbits was more cartilage-like than in the | 8978654 | [
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Maturation-dependent repair of untreated osteochondral defects in the rabbit knee joint. | adults (p < 0.05). In addition; bonding of the repair tissue to the adjacent cartilage was better in the young and adolescent than in the adult animals (p < 0.05). At 12 weeks; subchondral bone had formed in some young and adolescent repairs; but in no case in the adults. None of the repairs showed normal cartilage appearance; but formation of hyaline-like cartilage was common at 12 weeks. Irrespective of age or observation time; the repair site showed decreased stiffness and larger strain values compared t | 8978654 | [
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Maturation-dependent repair of untreated osteochondral defects in the rabbit knee joint. | o adjacent or control cartilage (p < 0.001) with no tendency for improvement over time. Younger animals showed a faster filling of the defect and an earlier specialization of the repair tissue than adult animals; but the mechanical quality of the regenerated tissue remained inferior to normal. | 8978654 | [
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An in vivo study of the biodegradation of the hydrophilic Mitrathane. | The present study analyzes the kinetics of the in vivo degradation of hydrophilic Mitrathane in the peritoneal cavity of mice over a period ranging from 1 to 180 days. The mechanical milling of the polyurethane films produced regularly flattened fragments that in vivo spontaneously oriented into piles. The morphological observations and analysis with the aid of an image analysis system demonstrated that after seven days of swelling the polymer fragments undergo a continuous degradation that leads to an irre | 8978655 | [
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An in vivo study of the biodegradation of the hydrophilic Mitrathane. | gular thinning and phagocytosis of the smaller fragments by macrophages with very little chronic inflammation response from surrounding tissues. | 8978655 | [
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The bone-bonding polymer Polyactive 80/20 induces hydroxycarbonate apatite formation in vitro. | The bone-bonding polymer known as Polyactive is a block copolymer composed of a polyethylene oxide (PEO) soft segment and a polybutylene terephthalate (PBT) hard segment. This study focuses on the in vitro induction of hydroxyapatite by Polyactive. Our results show that Polyactive is capable of inducing hydroxycarbonate apatite (HCAp) formation from a metastable calcium phosphate solution analogous to a physiological solution. In a 4-day incubation; the HCAp formation extended approximately 100 microm deep | 8978656 | [
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The bone-bonding polymer Polyactive 80/20 induces hydroxycarbonate apatite formation in vitro. | from the surface. A great number of globules about 1 microm large were found in the calcified Polyactive. These globules were composed of HCAp crystals embedded in the polymer matrix. There were so many globules in the surface that they connected with each other and formed a calcified layer. Next to the calcified layer was a zone where the globules were scattered. The calcified surface may have acted to promote HCAp growth from the solution; bringing about the formation of a HCAp layer on top of the calcifi | 8978656 | [
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The bone-bonding polymer Polyactive 80/20 induces hydroxycarbonate apatite formation in vitro. | ed layer. The transition of solid Polyactive into a Polyactive hydrogel in calcium phosphate solution permitted HCAp formation within the polymer. It is proposed that the COOH groups produced during hydrolysis of Polyactive play an important role in nucleating hydroxyapatite. A remarkable affinity of the PEO segment of the polymer for calcium ions may facilitate moving calcium and phosphate from the solution into the polymer for the growth of HCAp. | 8978656 | [
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Retinal pigment epithelium cells cultured on synthetic biodegradable polymers. | Alterations in the normal structure or functions of retinal pigment epithelium (RPE) can result in a number of ocular diseases. Implantation of RPE cells cultured on thin; biodegradable polymer films may provide a means of transplanting an organized sheet of RPE cells with distinct apical/basal characteristics for the restoration of normal RPE function. We have investigated the interactions of human RPE cells with different biodegradable polymer films to assess their suitability as substrates for RPE cultur | 8978657 | [
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Retinal pigment epithelium cells cultured on synthetic biodegradable polymers. | e. Four biodegradable polymers were used: low molecular weight (MW) 50:50 poly(DL-lactic-co-glycolic acid) (PLGA); high MW 50:50 PLGA; 75:25 PLGA; and poly(L-lactic acid) (PLLA). Polymer film substrates were manufactured using a solvent casting technique. Human fetal RPE cells (10-16 weeks gestational) were plated on the polymer substrates and the cultures assessed with respect to cell attachment and proliferation. Histological and immunohistochemical studies were performed on the cells after 8 days in cult | 8978657 | [
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Retinal pigment epithelium cells cultured on synthetic biodegradable polymers. | ure. RPE cells attached to all the polymers studied after 8 h in culture. After 8 h; 80.2 +/- 9.5% and 82.3 +/- 7.9% of the plated cells were attached to substrates of high MW 50:50 PLGA and 75:25 PLGA; respectively. The cells proliferated on all substrates; and there was about a threefold increase in cell number over the 8-day culture period on all the polymers studied. Immunohistochemistry after 8 days in culture demonstrated RPE cells labeled with a distinct reaction product for cytokeratin in the cell c | 8978657 | [
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Retinal pigment epithelium cells cultured on synthetic biodegradable polymers. | ytoplasm. All the polymers studied were suitable for RPE culture; however; high MW 50:50 PLGA and 75:25 PLGA proved to be the best in terms of manufacturing properties; cell attachment; and proliferation. These polymers can provide a suitable substrate for RPE cell culture and hold promise for the subretinal implantation of organized sheets of RPE cells. | 8978657 | [
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Development and characterization of a biodegradable polyphosphate. | A biodegradable polyphosphate polymer (Mn = 18;000; Mw/Mn = 3.2) matrix system was developed as a potential delivery vehicle for growth factors. As a model system; release of recombinant human osteogenic protein-1 (OP-1) from this polymer was evaluated. The polyphosphate was synthesized using a triethylamine catalyst in an argon environment; and characterized using elemental analysis; gel permeation chromatography (GPC); and Fourier transform infrared spectroscopy (FTIR). Degradation kinetics of the polypho | 8978658 | [
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Development and characterization of a biodegradable polyphosphate. | sphate polymer in phosphate-buffered saline (PBS) were represented by a second-order polynomial while degradation in bovine serum was linear with time. The polymer degraded faster in PBS than in bovine serum. In vitro release of OP-1 was also faster in PBS than in serum. Release kinetics of OP-1 in PBS and serum were represented by second-order polynomials. The OP-1 release from this physically dispersed polymeric matrix may be described by several possible mechanisms: diffusion; bulk polymer degradation; i | 8978658 | [
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Development and characterization of a biodegradable polyphosphate. | on complexation; and interactions among the protein (OP-1); polymer; proteins; and enzymes in the media. This polyphosphate may be an effective carrier for morphogens; growth factors; or other classes of bioactive molecules. | 8978658 | [
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Plasma protein adsorption and platelet adhesion onto comb-like PEO gradient surfaces. | Comb-like polyethylene oxide (PEO) surfaces were prepared on low-density polyethylene (PE). The comb-like PEO chain density was changed gradually along the sample lengths by corona discharge treatment with gradually increasing power and the following graft copolymerization of poly(ethylene glycol) monomethacrylate macromers (PEO-MA). The macromers with different PEO repeat unit; 1; 5; and 10; were used. The prepared comb-like PEO gradient surfaces were characterized by water contact angle; Fourier transform | 8978659 | [
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Plasma protein adsorption and platelet adhesion onto comb-like PEO gradient surfaces. | infrared spectroscopy in the attenuated total reflectance mode; and electron spectroscopy for chemical analysis. All these measurements indicated that the PEO chains are grafted on the PE surface with gradually increasing density of PEO. Plasma protein adsorption and platelet adhesion on the PEO gradient surfaces decreased with increasing PEO chain length and surface density. As observed by scanning electron microscopy; PEO10-MA-grafted surface with high PEO density was very effective in preventing protein | 8978659 | [
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LPS increases biomaterial degradation by human monocytes in vitro. | Different cell lines are involved during an immunological reaction; principally lymphocytes and monocytes. Monocyte/macrophage cells; which are among the first to appear in wound-healing and infection sites; are largely implicated in phagocytosis and could be involved in calcium-phosphate degradation. Their role in these processes may relate to cytokine secretions and/or their sensitivity to certain cytokines. We tested the behavior of human monocytes placed on the surface of biphasic calcium-phosphate (BCP | 8978660 | [
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LPS increases biomaterial degradation by human monocytes in vitro. | ) tablets in the presence of two lipopolysaccharide (LPS) concentrations. After short-term culture (48 h); cytokine release (IL-1beta; IL-6) was measured by ELISA; and morphological cell events and biomaterial degradation were observed in scanning electron microscopy. BCP surface pits were noted near cells stimulated by 0.5 microg/mL LPS but were not apparent with 10 microg/mL LPS. The number of lacunae on BCP was increased after LPS treatment of human monocytes. An upmodulation of IL-1beta and IL-6 (in cul | 8978660 | [
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LPS increases biomaterial degradation by human monocytes in vitro. | ture medium) released by LPS-activated human monocytes was observed; indicating good cell stimulation. This study demonstrates that LPS-activated human monocytes can degrade the surface of calcium-phosphate ceramic and confirms the role of human monocytes in biomaterial degradation. | 8978660 | [
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The effect of Ca-P plasma-sprayed coatings on the initial bone healing of oral implants: an experimental study in the goat. | The response of bone of low density to uncoated; fluorapatite (FA); hydroxyapatite (HA); and hydroxyapatite heat treated (HAHT) plasma-sprayed coated implants was investigated 3 and 6 months after installation. Forty-eight threaded implants of commercially pure titanium were inserted into the maxilla of twelve goats according to a split-plot design. One goat died shortly after installation of the implants; five goats were sacrificed 3 months after installation; and the other six goats 6 months after install | 8978661 | [
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The effect of Ca-P plasma-sprayed coatings on the initial bone healing of oral implants: an experimental study in the goat. | ation. Histological evaluation revealed no difference in bone reaction between the 3- and 6-month implantation periods. In addition; probably due to the wide inter- and intra-animal variability; no significant difference between the 3- and the 6-month periods could be observed in the histomorphometrical measurements performed. Further; no significant differences were found in bone reaction among the various implant materials. Finally; qualitatively it appeared that all coatings showed reduction in coating t | 8978661 | [
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The effect of Ca-P plasma-sprayed coatings on the initial bone healing of oral implants: an experimental study in the goat. | hickness and that such reductions were most pronounced for the HA coatings. | 8978661 | [
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Application of tertiary amines with reduced toxicity to the curing process of acrylic bone cements. | 4-Dimethylaminobenzyl alcohol (DMOH) and 4-dimethylaminobenzyl methacrylate (DMMO) were used as the activators in the benzoyl peroxide initiated redox polymerization for the preparation of acrylic bone cement based on poly(methylmethacrylate) beads of different particle size. The residual monomer content of the cured cements was about 2 wt %; independent of the redox system used in the polymerization; indicating that the activating effect of the tertiary aromatic amines DMOH or DMMO was sufficient to reach | 8978662 | [
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Application of tertiary amines with reduced toxicity to the curing process of acrylic bone cements. | a polymerization conversion similar to that obtained with the benzoyl peroxide (BPO) N;N-dimethyl-4-toluidine (DMT) system. The BPO/DMOH and BPO/DMMO redox systems provided exotherms of decreasing peak temperature and increasing setting time; and the cured materials presented higher average molecular weight and similar glass transition temperatures in comparison with those obtained when DMT was used as the activator. In addition; these activators are three times less toxic than the classical DMT. | 8978662 | [
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Chemical chaperones interfere with the formation of scrapie prion protein. | The fundamental event in prion diseases involves a conformational change in one or more of the alpha-helices of the cellular prion protein (PrP(C)) as they are converted into beta-sheets during the formation of the pathogenic isoform (PrP(Sc)). Here; we show that exposure of scrapie-infected mouse neuroblastoma (ScN2a) cells to reagents known to stabilize proteins in their native conformation reduced the rate and extent of PrP(Sc) formation. Such reagents include the cellular osmolytes glycerol and trimethy | 8978663 | [
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