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Differences in domain structure between pericellular matrix and plasma fibronectins as revealed by domain-specific antibodies combined with limited proteolysis and S-cyanylation: a preliminary note. | Differences in domain structure between human fibronectins obtained from pericellular matrix and plasma have been revealed by limited proteolysis and S-cyanylation; followed by identification of each domain with domain-specific antibodies. Although the overall domain structure is similar between pericellular and plasma fibronectins; the fragments derived from the COOH-terminal region of these fibronectins; which were defined by specific antibodies; exhibited clear differences in their molecular weights and | 6360169 | [
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Insulin-stimulated phosphorylation of the insulin receptor precursor. | The alpha and beta subunits of the insulin receptor; Mr = 135K and 95K; appear to be synthesized via a single polypeptide precursor of Mr = 190K. We have investigated whether insulin stimulates the phosphorylation of this proreceptor; as is the case with mature receptor. Rat liver endoplasmic reticulum membranes were solubilized in Triton X-100 and chromatographed sequentially on wheat-germ agglutinin-agarose and lentil lectin-agarose columns. Phosphorylation of the lentil eluate with [gamma 32P]ATP reveale | 6360168 | [
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Insulin-stimulated phosphorylation of the insulin receptor precursor. | d an insulin-stimulated phosphoprotein of Mr = 192K; which was recognized by antireceptor antibody; compatible with the receptor precursor. This suggests that further processing of the Mr = 190K insulin receptor precursor is not necessary for insulin binding; kinase activation; and receptor phosphorylation. | 6360168 | [
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Sequence homology between prokaryotic and eukaryotic forms of serine hydroxymethyltransferase. | The sequence of tryptic and chymotryptic peptides from cytosolic and mitochondrial rabbit liver serine hydroxymethyltransferase are compared to the proposed sequence of a protein coded for by the glyA gene of Escherichia coli. The E. coli glyA gene is believed to code for serine hydroxymethyltransferase. Extensive sequence homology between these peptides were found for the proposed E. coli enzyme in the aminoterminal two-thirds of the molecule. All three proteins have identical sequences from residue 222-23 | 6360170 | [
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Sequence homology between prokaryotic and eukaryotic forms of serine hydroxymethyltransferase. | 1. This sequence is known to contain the lysyl residue which forms a Schiff's base with pyridoxal-P in the two rabbit liver enzymes. These results support the interpretation that the proposed sequence of E. coli serine hydroxymethyltransferase is correct. The data also show that cytosolic and mitochondrial serine hydroxymethyltransferase are homologous proteins. | 6360170 | [
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The possible involvement of prostaglandin F2 alpha in the stimulation of muscle protein synthesis by insulin. | The addition of insulin (8 ng/ml) in vitro to muscles from fasted rabbits increased protein synthesis (+80%) to a value similar to that found in muscles from fed donors. The addition of either indomethacin or meclofenamate completely blocked this effect of insulin. Muscles from fasted rabbits released less prostaglandin (PG)F2 alpha into the medium and the presence of insulin increased and indomethacin and meclofenamate reduced PGF2 alpha release. Other conditions (work load and leucocyte pyrogen) which inc | 6360171 | [
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The possible involvement of prostaglandin F2 alpha in the stimulation of muscle protein synthesis by insulin. | rease protein synthesis in muscle also stimulate PGF2 alpha release. As both arachidonic acid and PGF2 alpha in themselves increase protein synthesis we suggest that accelerated phospholipolysis and PG synthesis have a general role in the control of muscle protein turnover. | 6360171 | [
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Identification of bacteriophage T4D gene 29 product, a baseplate hub component, as a folylpolyglutamate synthetase. | An assay for folylpolyglutamate synthetase activity in extracts of uninfected and bacteriophage T4D-infected Escherichia coli B has been developed. T4D infection induced the formation of a new synthetase raising the total synthetase activity three-fold. Extracts obtained after infection with T4 gene 51; 27 or 28 amber mutants showed increased synthetase activities while extracts obtained from cells infected with a T4D gene 29 amber mutant did not show any increase in synthetase activity. The phage-induced s | 6360172 | [
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Identification of bacteriophage T4D gene 29 product, a baseplate hub component, as a folylpolyglutamate synthetase. | ynthetase was found to copurify with the gene 29 product and a 100-fold purified synthetase of molecular size of 74;000 daltons has been obtained. The purified synthetase has a folate substrate specificity different from the host synthetase since it added glutamate residues to dihydrofolate as well as to the usual tetrahydrofolate substrate. | 6360172 | [
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Yeast pheromone alpha-factor is synthesized as a high molecular weight precursor. | The sex pheromone alpha-factor of Saccharomyces cerevisiae; a tridecapeptide of approx. 1;700 molecular weight; was found to be synthesized in vivo as a high molecular weight precursor of Mr = 28;000. Inhibition of N-linked glycosylation by tunicamycin leads to three precursor species of lower molecular weight indicating three carbohydrate residues linked to the alpha-factor precursor molecule. A molecular weight of 18;000 was determined for the unglycosylated molecule. | 6360173 | [
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Essentiality of insulin and prolactin for accumulation of rat casein mRNAs. | Fetal calf serum (20%) does not substitute for insulin in terms of enhanced accumulation of rat casein mRNAs; but it can sustain the mammary tissue in culture. This property of fetal calf serum was utilized to show that in the presence of cortisol and 1) insulin or 2) prolactin; the delayed addition of prolactin or insulin; respectively; produced similar enhancement of 25 K and 42 K casein mRNAs. The results indicate that both insulin and prolactin are required for accumulation of rat casein mRNAs; the accu | 6360174 | [
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Sites of action of Ca2+ channel inhibitors. | Ca2+ channel inhibitors are viewed as a subgroup of Ca2+ antagonists. Most of the currently used Ca2+ channel inhibitors are thought to act by reducing Ca2+ entry into the cell through Ca2+ channels. There is substantial electrophysiological evidence that the major site of action of verapamil; nifedipine and diltiazem in cardiac cells is a sarcolemmal Ca2+ channel. Cytosolic sites of action may contribute to their effects but probably only at higher than therapeutic concentrations. The recent ligand binding | 6360175 | [
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Sites of action of Ca2+ channel inhibitors. | studies also tend to support the view that the sarcolemma is the site of action of Ca2+ channel inhibitors in smooth muscle. High affinity binding sites for 1;4-dihydropyridines without any established function are found in fast skeletal muscle and some neuronal membranes. The binding of [3H]nitrendipine to membranes from cardiac; skeletal and smooth muscle; and from brain is saturable; reversible and of high affinity; it is sensitive to cations and other drugs that interact with Ca2+ channels. Inhibition | 6360175 | [
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Sites of action of Ca2+ channel inhibitors. | of [3H]nitrendipine binding and blockade of K+ responses in guinea pig ileum by 1;4-dihydropyridines are well correlated; supporting the view that the observed binding is to Ca2+ channel. In contrast; blockade of Ca2+ channels in cardiac and skeletal muscle and in brain synaptosomes occurs only at higher concentrations than needed to saturate the high affinity binding sites. The therapeutic success of Ca2+ channel inhibitors in the treatment of angina pectoris; hypertension; peripheral vascular diseases; an | 6360175 | [
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Sites of action of Ca2+ channel inhibitors. | d many other disease entities is based on selective inhibition of Ca2+ entry into smooth muscle cells. The specificity of some of these drugs for Ca2+ channels in different cell types; organs; or vascular beds is probably determined by receptor modulation and the effect of reflex mechanisms; which in turn determine the indications for their therapeutic use. | 6360175 | [
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In vivo hydroxylation of 3H-acetanilide--evaluation of a new radiospirometric method in the rat. | The proposed in vivo methodology for the investigation of hydroxylation rates consists of of the i.v. administration of tritiated substrates and the collection of tritiated water (HTO) from exhaled air as a measure of HTO accumulation in body water. Specifically; HTO was assessed in exhaled water after i.v. administration of 3H-acetanilide. Over a wide range the half lives of accumulation of HTO in exhaled water (T50) were almost identical with the half lives of elimination of 3H-acetanilide in blood; evalu | 6360176 | [
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In vivo hydroxylation of 3H-acetanilide--evaluation of a new radiospirometric method in the rat. | ated by an inverse isotope dilution method (r = 0.96; N = 18). Average T50 amounted to 29 min in controls; was reduced to 20 min after enzyme induction by phenobarbital or 3-methylcholanthrene; and prolonged to 45; 46 and 66 min after bile duct ligation; portacaval shunt and a single dose of ethanol; respectively. It is concluded that the chosen pharmacokinetic approach corrects for the NIH-shift and the results adequately reflect changes in acetanilide hydroxylation related to enzyme induction or inhibitio | 6360176 | [
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In vivo hydroxylation of 3H-acetanilide--evaluation of a new radiospirometric method in the rat. | n and to liver pathology. | 6360176 | [
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Drug protein conjugates--VI. Role of glutathione in the metabolism of captopril and captopril plasma protein conjugates. | Previous metabolic studies of captopril suggest that the rapid dissociation of captopril-plasma protein conjugates in vivo is dependent upon endogenous thiols such as glutathione and cysteine. Consistent with this hypothesis; we have found that cysteine (0.06-3 mM) and glutathione (0.02-1 mM) cleave 14C-captopril-plasma protein conjugates in vitro. Dissociation of the drug-protein conjugate was accompanied by formation of the corresponding mixed disulphide which indicates that the reaction proceeds via a sp | 6360177 | [
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Drug protein conjugates--VI. Role of glutathione in the metabolism of captopril and captopril plasma protein conjugates. | ontaneous thiol-disulphide interchange. Administration of high doses (50-300 mg/kg) of CP produced a time-dependent and dose-dependent decrease in hepatic glutathione concentrations in the mouse and the rat. The depletion of glutathione observed was similar to that produced by equimolar doses of D-penicillamine and paracetamol. Acute and chronic (7 days) administration of captopril (100 mg/kg) produces the same (11-12%) depletion of hepatic glutathione. However; changes in liver function as determined by el | 6360177 | [
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Drug protein conjugates--VI. Role of glutathione in the metabolism of captopril and captopril plasma protein conjugates. | evation of serum glutamic-pyruvic transaminase was only observed at doses of 200 and 300 mg/kg. Thus; although thiol-disulphide interactions between captopril and plasma proteins may contribute to the perturbation of hepatic glutathione concentrations; it is unlikely that this process will be of toxicological significance during therapeutic administration of captopril. | 6360177 | [
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Effect of lymphoplasmapheresis on clinical indices and T cell subsets in rheumatoid arthritis. A double-blind controlled study. | The effects of lymphoplasmapheresis on immunologic indices; including T cell subsets; and on clinical parameters of rheumatoid arthritis were evaluated in a controlled double-blind trial. Twenty patients were randomized to receive either 6 lymphoplasmapheresis sessions or a seemingly identical control procedure over a 3-week period. Lymphoplasmapheresis produced significant reduction in serum levels of total lymphocytes; erythrocyte sedimentation rate; C-reactive protein; and IgG. These serologic measures r | 6360178 | [
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Effect of lymphoplasmapheresis on clinical indices and T cell subsets in rheumatoid arthritis. A double-blind controlled study. | eturned to baseline 5 weeks after lymphoplasmapheresis. No change in the imbalance of T cell subsets (increased helper/suppressor ratio) was observed. No changes in the serologic measures; except IgA; were observed in the control group. An improvement in some of the clinical parameters was observed in both the lymphoplasmapheresis and control groups. A rebound above baseline values for several parameters was observed in both the lymphoplasmapheresis and the sham apheresis groups. | 6360178 | [
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Interaction of polymorphonuclear leukocytes with immune complexes trapped in rheumatoid articular cartilage. | When rheumatoid articular cartilage samples were incubated with normal polymorphonuclear leukocytes (PMN) in vitro; large numbers of PMN were seen attached to the articular surface. As observed by electron microscopy; significant numbers of these cells invaded the cartilage tissue and phagocytosed amorphous material which presumably contained immune complexes. In control cartilage from osteoarthritic; pyogenic; and normal subjects; only a few PMN were attached to the articular surface after incubation with | 6360179 | [
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Interaction of polymorphonuclear leukocytes with immune complexes trapped in rheumatoid articular cartilage. | PMN. These results demonstrate that immune complexes trapped in the superficial region of the rheumatoid articular cartilage may play an important role in the destruction of cartilage by the release of lysosomal enzymes from PMN. | 6360179 | [
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Sulfonyliminoimidazolidines, a new class of oral hypoglycemic agents. 3. Hypoglycemic activity and mode of action of 1-[p-[2-(crotonylamino)-ethyl]-phenylsulfonyl]-3-cyclohexyl-2-imino- imidazolidine (CGP 11 112). | 1-[p-[2-(Crotonylamino)-ethyl]-phenylsulfonyl]-3-cyclohexyl-2-imino- imidazolidine (CGP 11 112) is a representative of a new class of oral hypoglycemic agents. It lowers blood glucose in normal animals and in streptozocin (streptozotocin)-diabetic rats. In normal mice; rats and dogs the hypoglycemic effect is more potent (range 3-30 times) and has a shorter duration than that of tolbutamide. CGP 11 112 increases plasma insulin after oral administration in normal animals and stimulates release of insulin in | 6360181 | [
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Sulfonyliminoimidazolidines, a new class of oral hypoglycemic agents. 3. Hypoglycemic activity and mode of action of 1-[p-[2-(crotonylamino)-ethyl]-phenylsulfonyl]-3-cyclohexyl-2-imino- imidazolidine (CGP 11 112). | perifused rat islets in vitro (sulfonylurea-like effect). In streptozocin-diabetic rats CGP 11 112 decreases blood glucose with a potency comparable to that of phenformin. In contrast to phenformin; CGP 11 112 does not increase blood lactate in diabetic rats or inhibit intestinal absorption of glucose. An effect of CGP 11 112 on gluconeogenesis and glycogenolysis could not be demonstrated. It is suggested that the hypoglycemic activity in streptozocin-diabetic rats may be due to stimulation of glucose efflu | 6360181 | [
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Sulfonyliminoimidazolidines, a new class of oral hypoglycemic agents. 3. Hypoglycemic activity and mode of action of 1-[p-[2-(crotonylamino)-ethyl]-phenylsulfonyl]-3-cyclohexyl-2-imino- imidazolidine (CGP 11 112). | x from the circulation. In vitro; CGP 11 112 inhibits glucose oxidation and lipolysis in isolated rat fat cells. | 6360181 | [
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The pathogenesis of group A streptococcal cell wall-induced polyarthritis in the rat. Comparative studies in arthritis resistant and susceptible inbred rat strains. | This report addresses the host mechanisms which regulate susceptibility and resistance to group A streptococcal cell wall-induced polyarthritis in the rat. We have compared cell wall tissue distribution and persistence following a single intraperitoneal injection of an aqueous suspension of the cell walls into arthritis susceptible LEW/N and resistant F344/N female rats. Selected aspects of the induced inflammatory reaction were also compared. Our results suggest that development of chronic erosive polyarth | 6360180 | [
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The pathogenesis of group A streptococcal cell wall-induced polyarthritis in the rat. Comparative studies in arthritis resistant and susceptible inbred rat strains. | ritis in this model is dependent; in variable degrees; upon 1) deposition and persistence of the cell walls in synovial tissues; and 2) a relative inability of arthritis susceptible rats to neutralize the proinflammatory properties of the cell walls. | 6360180 | [
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Influence of external and covert food stimuli on insulin secretion in obese and normal persons. | Exposure to food produces an increase in insulin secretion that is more pronounced in obese than in normal persons. We sought to replicate this finding as well as to determine whether insulin secretion could be influenced by thoughts about food and eating. Normal and obese subjects were presented in counterbalanced order with an external food stimulus or instructions to think about food and eating. Levels of insulin; salivary output; and hunger ratings were obtained prior to and immediately following stimul | 6360189 | [
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Influence of external and covert food stimuli on insulin secretion in obese and normal persons. | us presentation. All subjects displayed higher insulin levels following stimulus presentation; with the values for the obese higher than for normals. Although the insulin secretion of the obese was greater in response to the covert as opposed to the external stimulus; the difference was not significant. Salivary output following stimulus presentation was higher for the obese than normals; with hunger ratings increasing in both groups. These data support an expanded role of insulin as an appetite-inducing me | 6360189 | [
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The teeth and infective endocarditis. | During 1981 and 1982 544 cases of infective endocarditis were investigated retrospectively by means of a questionnaire. Only 13.7% had undergone any dental procedure within three months of the onset of the illness; and in 42.5% there was no known cardiac abnormality before the onset of the disease. Furthermore; the number of cases occurring annually was about the same as or more than it was before the introduction of penicillin. The mouth and nasopharynx were the most likely sources of the commonest organis | 6360190 | [
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The teeth and infective endocarditis. | m; Streptococcus viridans; and it is suggested that it is not dental extractions themselves which are of importance but good dental hygiene. In most patients with infective endocarditis the portal of entry of the organism whatever its nature cannot be identified. If this is so antibiotics are being given to only a small proportion of those at risk; and this would explain why the number of cases is much the same as it was before the introduction of penicillin. Furthermore; the large proportion of patients wi | 6360190 | [
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The teeth and infective endocarditis. | th no known previous cardiac abnormality adds to the difficulty of providing effective prophylaxis. The evidence suggests that antibiotic prophylaxis should still be given before dental procedures; and a schedule is appended. Much more importance should be given; however; to encouraging people to seek better routine dental care. We also believe that doctors and dentists should appreciate that the pattern of the disease has changed considerably in the past 50 years and that the information given here warrant | 6360190 | [
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Electrophysiological abnormalities in the transplanted human heart. | Fourteen relatively long term survivors of cardiac transplantation underwent systematic electrophysiological evaluation and ambulatory electrocardiographic monitoring. Six patients had prolonged conduction intervals during sinus rhythm. Sinus node function could be assessed in all donor atria and in 10 recipient atria. Sinus node recovery times were prolonged in four of the donor atria and in six recipient atria. In the donor atria abnormalities of sinus node automaticity were invariably associated with abn | 6360191 | [
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Electrophysiological abnormalities in the transplanted human heart. | ormalities of sinoatrial conduction. Four patients showed functional duality of atrioventricular nodal conduction during programmed extrastimulation; but no patient developed re-entrant arrhythmia. During ambulatory electrocardiographic monitoring no pronounced tachyarrhythmias were recorded. Three patients showed abnormalities of sinus node impulse formation. All three patients had abnormal sinus node recovery times during their electrophysiological study. Long term survivors of cardiac transplantation hav | 6360191 | [
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Electrophysiological abnormalities in the transplanted human heart. | e a high incidence of electrophysiological abnormalities. Abnormalities of donor sinus node function are probably of clinical significance. The clinical significance of abnormalities detected within the atrioventricular conduction system of the denervated heart remains to be elucidated. | 6360191 | [
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Routine immunofluorescent and histochemical analysis of bone marrow involvement of lymphoma/leukaemia: the use of cryostat sections. | Enzyme histochemical and immunohistological (immuno-fluorescence and -peroxidase) techniques have been routinely used for investigating over 70 normal and pathological bone marrow samples. This recently standardized diagnostic procedure is very quick and can be performed in a few hours. In 6 cases the clinical diagnosis of leukaemia/lymphoma has become apparent only after the immunohistological analysis of the bone marrow. In 6 other cases the information about the staging of B cell malignancies was superio | 6360192 | [
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Routine immunofluorescent and histochemical analysis of bone marrow involvement of lymphoma/leukaemia: the use of cryostat sections. | r in the frozen biopsies to the paraffin embedded preparations. Amongst many other features the monoclonality of B CLL/lymphomas; the special features of B CLL infiltrates (RFA-1+; Leu-1+; HLA-DR+; SmIg+); follicular lymphoma deposits (containing follicular dendritic cells) and non-T; non-B acute lymphoblastic leukaemic blasts (terminal transferase+; HLA-DR+) as well as the sometimes conspicuous presence of infiltrating normal T cells could be clearly and reproducibly demonstrated. | 6360192 | [
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Sister chromatid exchange in lymphocytes from renal transplant recipients with and without cancer. | The frequency of sister chromatid exchange (SCE) was measured in peripheral blood lymphocytes from control; healthy subjects and immunosuppressed recipients of cadaveric donor kidneys with and without skin cancer. The mean SCE frequency in 43 control subjects was 9.2 per cell (range 5.4-12.3). In 30 transplant recipients with no history or evidence of cancer the mean SCE rate was 10.3 per cell (range 5.8-24.5); four (13%) of these patients had a mean SCE frequency outside the control range. In 7 transplant | 6360193 | [
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Sister chromatid exchange in lymphocytes from renal transplant recipients with and without cancer. | recipients with skin cancer; the mean SCE frequency was 14.3 per cell (range 9.1-19.9). This was significantly (P less than 0.01) higher than the mean value of control subjects. The mean SCE frequencies in 3 of these 7 patients fell within the control range and in 4 of these patients was above the control range. These results suggest that some immunosuppressed kidney transplant recipients are liable to chromosomal damage. | 6360193 | [
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Distribution of major histocompatibility antigens in normal skin. | The distribution of major histocompatibility antigens HLA-A;B;C (HLA); beta 2-microglobulin (beta 2m); and Ia-like antigens (HLA-DR; Ia) in normal skin was studied in frozen tissue sections by a four-step immunoperoxidase method and an avidin-biotin method employing monoclonal antibodies. HLA and beta 2m were present on the basal and spinous keratinocytes of the epidermis; on the outer root sheath epithelium in the infundibulum of the hair follicle; and on the excretory sebaceous duct epithelium. Ia-positiv | 6360195 | [
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Distribution of major histocompatibility antigens in normal skin. | e dendritic cells were found in the epidermis and hair follicles; but they were more frequent in the infundibulum and isthmus of the hair follicle than in its inferior portion or in the epidermis. In the straight eccrine duct; HLA and beta 2m-positivity was most striking in its lower portion. In the superficial duct; there was a less intense staining using the four-step procedure; but when an avidin-biotin method was used; the difference was less apparent. In contrast; the acrosyringial epithelium was marke | 6360195 | [
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Distribution of major histocompatibility antigens in normal skin. | dly Ia-positive with decreasing intensity of staining as the duct penetrated the dermis. No HLA or Ia antigens were identified in eccrine glands and apocrine glands. Eccrine glands were slightly beta 2m-positive. HLA and beta 2m were uniformly present in non-dilated and dilated intradermal apocrine ducts. | 6360195 | [
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The risks of antenatal subcutaneous heparin prophylaxis: a controlled trial. | The risks of long-term antenatal subcutaneous heparin therapy were assessed in a small controlled trial of prophylaxis of thromboembolism. Forty patients with a documented history of previous thromboembolism were randomly allocated either to receive heparin (10000 i.u. subcutaneously twice daily) throughout pregnancy and labour or to receive no treatment (control group). All patients were treated with heparin (8000 i.u. twice daily) for 6 weeks after delivery from the first postnatal day. There appeared to | 6360198 | [
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The risks of antenatal subcutaneous heparin prophylaxis: a controlled trial. | be no increased risk of antenatal or postnatal bleeding associated with subcutaneous heparin; but one patient in the control group developed a deep vein thrombosis and one in the treatment group developed severe debilitating osteopenia. The withholding of epidural analgesia may have contributed to both maternal and fetal morbidity in the treatment group. There was one abortion in each group but no other fetal or neonatal losses although more babies from the treated group entered the special care baby unit. | 6360198 | [
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The risks of antenatal subcutaneous heparin prophylaxis: a controlled trial. | Although the numbers are too small for statistical analysis; the findings indicate that the use of long-term low-dose subcutaneous heparin is not without complications and there is need for a larger; multicentre trial to allow precise quantification of fetal and maternal risks against the risk of recurrent thromboembolism. | 6360198 | [
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Reliability of cytological follow-up after conization of the cervix; a comparison of three surgical techniques. | A retrospective analysis was made of 154 consecutive conizations between 1974 and 1982. Three surgical methods were compared: (A) Sturmdorf sutures; (B) interrupted vertical sutures; and (C) an 'open' method using only cauterization and no additional stitches. The mean blood loss during conization was not reduced by ligation of descending branches of the uterine arteries. Post-conization haemorrhage occurred in only 3% and did not differ between the three groups. Cervical smears with only ectocervical squam | 6360199 | [
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Reliability of cytological follow-up after conization of the cervix; a comparison of three surgical techniques. | ous epithelium or too little material to allow diagnosis were considered inadequate. Unreliable follow-up was defined as inadequacy of more than 25% of a patient's smears. Thirty per cent of all follow-up smears were inadequate (group A 33%; B 41%; C 16%). In 46% of the patients; cytological follow-up was unreliable (group A 48%; B 61%; C 18%) and additional measures such as dilatation of the cervix; endocervical curettage; or hysterectomy were required. The differences between group C and the other two gro | 6360199 | [
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Reliability of cytological follow-up after conization of the cervix; a comparison of three surgical techniques. | ups were statistically significant (P less than 0.01; chi 2 test). | 6360199 | [
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