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Upload techniques_data - techniques_data.csv
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techniques_data - techniques_data.csv
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point,technique,relationship/description/definition/use,factor/label/component/method/mechanism
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Migration rate dependency,Electrophoresis,Directly proportional to rate of movement,Charge of the molecule
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Migration rate dependency,Electrophoresis,Inversely proportional to rate of movement,Size of the molecule
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Migration rate dependency,Electrophoresis,Increased current increases migration rate,Electrical field
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Migration rate dependency,Electrophoresis,Increased ionic strength decreases migration rate,Ionic strength of buffer
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Migration rate dependency,Electrophoresis,Decreased pH slows migration,pH of buffer
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Migration rate dependency,Electrophoresis,Inversely proportional to migration rate,Viscosity of supporting medium
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Migration rate dependency,Electrophoresis,High temperature can denature protein and slow migration,System temperature
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Analytic electrophoretic procedures,Electrophoresis,Proteins are amphoteric...,Protein electrophoresis
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Analytic electrophoretic procedures,Electrophoresis,Similar to protein electrophoresis...,Isoenzyme electrophoresis
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Components,Immunoassay,A substance that can elicit an immune response...,Antigen
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Components,Immunoassay,An immunoglobulin formed in response to a foreign substance...,Antibody
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Immunochemical labels,Immunoassay,"Attached to the antibody, allowing colorimetric quantitation...",Enzyme labels
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Immunochemical labels,Immunoassay,"Emit photons when excited, used in fluorescence-based detection...",Fluorescent labels
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Immunochemical labels,Immunoassay,Release energy in the form of visible light upon returning to ground state...,Chemiluminescent labels
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Immunochemical labels,Immunoassay,"Emit radiation (gamma rays) upon decay, detected for quantitation...",Radioisotope labels
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Methodologies,Immunoassay,Enzyme-based,ELISA
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Methodologies,Immunoassay,Enzyme-based,EIA/EMIT
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Methodologies,Immunoassay,Fluorescence-based,FPIA
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Methodologies,Immunoassay,Fluorescence-based,FIA
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Methodologies,Immunoassay,Radiation-based,RIA
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Methodologies,Immunoassay,Radiation-based,IRMA
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Mechanisms of separation,Chromatography,Based on the competition between the sample and the mobile phase for binding sites...,Adsorption chromatography
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Mechanisms of separation,Chromatography,Depends on the solubility of the solute in nonpolar or polar solvents...,Partition chromatography
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Mechanisms of separation,Chromatography,Involves separation of solutes by their size and charge of the ionic species present...,Ion-exchange chromatography
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Chromatographic procedures,Chromatography,Semi-quantitative screening test...,Thin-layer chromatography (TLC)
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Chromatographic procedures,Chromatography,Provides quantitative results...,High-performance liquid chromatography (HPLC)
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