questions: query related to plant protection in ash gourd 
 answers: continue spraying of saaf for two more dose at 5 days interval @ 2 gram per litre water
 perform ooze test at home to check whether it is bacterial infection or not. 
ooze test: dip the affected part in a glass of water and keep it for 30 minutes. if ooze comes out and the water become yellowish white, then spray streptomycin/ streptocyclin (1g/5 lit of water), near the root zone 3 times at 5 days interval .